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TMEM147 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol TMEM147
Entrez Gene ID 10430
Full Name transmembrane protein 147
Synonyms NIFIE14
General protein information
Preferred Names

transmembrane protein 147

Names

transmembrane protein 147
protein NIFIE 14
seven transmembrane domain protein
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

19

19q13.12
Disorder MIM:

613585


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001242598.1 NP_001229527.1 transmembrane protein 147 isoform 3
NM_032635.4 NP_116024.1 transmembrane protein 147 isoform 1
NM_001242597.1 NP_001229526.1 transmembrane protein 147 isoform 2
NM_032635.3 NP_116024.1 transmembrane protein 147 isoform 1
NM_001242598.2 NP_001229527.1 transmembrane protein 147 isoform 3
NM_001242597.2 NP_001229526.1 transmembrane protein 147 isoform 2

Pathways from BioSystems


Homologies


Mus musculus (house mouse) Tmem147 NP_081491.2
Danio rerio (zebrafish) tmem147 NP_001002484.1
Caenorhabditis elegans (roundworm) ZK418.5 NP_498544.1
Homo sapiens (human) TMEM147 NP_116024.1
TMEM147 XP_001095445.1
Rattus norvegicus (Norway rat) Tmem147 NP_001033583.1
Oryza sativa (rice) Os03g0766000 NP_001051379.2
Canis lupus familiaris (dog) TMEM147 XP_533692.1
Bos taurus (cattle) TMEM147 NP_001029796.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP008757 XP_314877.2
Drosophila melanogaster (fruit fly) CG5861 NP_609789.1
Arabidopsis thaliana (thale cress) AT1G47640 NP_564509.1

Gene Ontology


Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Transmembrane protein 147 (TMEM147) is a novel component of the Nicalin-NOMO protein complex.
Dettmer U, Kuhn PH, Abou-Ajram C, Lichtenthaler SF, Kr?ger M, Kremmer E, Haass C, Haffner C
The Journal of biological chemistry285(34)26174-81(2010 Aug)

Transcriptome analysis of human gastric cancer.
Oh JH, Yang JO, Hahn Y, Kim MR, Byun SS, Jeon YJ, Kim JM, Song KS, Noh SM, Kim S, Yoo HS, Kim YS, Kim NS
Mammalian genome : official journal of the International Mammalian Genome Society16(12)942-54(2005 Dec)

Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region.
Lehner B, Semple JI, Brown SE, Counsell D, Campbell RD, Sanderson CM
Genomics83(1)153-67(2004 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TMEM147 is a novel core component of the Nicalin-NOMO complex, further emphasizing its similarity with gamma-secretase.
Title: Transmembrane protein 147 (TMEM147) is a novel component of the Nicalin-NOMO protein complex.

The following TMEM147 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEM147 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu55834 NM_001242597.2
Latest version!		 Homo sapiens transmembrane protein 147 (TMEM147), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu55915 NM_032635.4
Latest version!		 Homo sapiens transmembrane protein 147 (TMEM147), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu06928 NM_001242598.2
Latest version!		 Homo sapiens transmembrane protein 147 (TMEM147), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
View Old Accession Versions >>
OHu55915 NM_032635.3 Homo sapiens transmembrane protein 147 (TMEM147), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu06928 NM_001242598.1 Homo sapiens transmembrane protein 147 (TMEM147), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu55834 NM_001242597.1 Homo sapiens transmembrane protein 147 (TMEM147), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu55834
Clone ID Related Accession (Same CDS sequence) NM_001242597.1 , NM_001242597.2
Accession Version NM_001242597.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 528bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-06-24
Organism Homo sapiens(human)
Product transmembrane protein 147 isoform 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM423809.1, BG697070.1, AW473288.1 and AW130399.1. Transcript Variant: This variant (2) lacks a splice site in the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.47134.1, SRR1163658.279395.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
ATGCTGTTCT TGGCCACTTT CTTTCCCACC TGGGAAGGCG GCATCTATGA CTTCATTGGG 
GAGTTCATGA AGGCCAGCGT GGATGTGGCA GACCTGATAG GTCTAAACCT TGTCATGTCC 
CGGAATGCCG GCAAGGGAGA GTACAAGATC ATGGTTGCTG CCCTGGGCTG GGCCACTGCT 
GAGCTTATTA TGTCCCGCTG CATTCCCCTA TGGGTCGGAG CCCGGGGCAT TGAGTTTGAC 
TGGAAGTACA TCCAGATGAG CATAGACTCC AACATCAGTC TGGTCCATTA CATCGTCGCG 
TCTGCTCAGG TCTGGATGAT AACACGCTAT GATCTGTACC ACACCTTCCG GCCAGCTGTC 
CTCCTGCTGA TGTTCCTCAG TGTCTACAAG GCCTTTGTTA TGGAGACCTT CGTCCACCTC 
TGCTCGCTGG GCAGTTGGGC AGCTCTACTG GCCCGAGCAG TGGTAACGGG GCTGCTGGCC 
CTCAGCACTT TGGCCCTGTA TGTCGCCGTT GTCAATGTGC ACTCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001229526.1
CDS359..886
Translation

Target ORF information:

RefSeq Version NM_001242597.1
Organism Homo sapiens(human)
Definition Homo sapiens transmembrane protein 147 (TMEM147), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001242597.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGCTGTTCT TGGCCACTTT CTTTCCCACC TGGGAAGGCG GCATCTATGA CTTCATTGGG 
GAGTTCATGA AGGCCAGCGT GGATGTGGCA GACCTGATAG GTCTAAACCT TGTCATGTCC 
CGGAATGCCG GCAAGGGAGA GTACAAGATC ATGGTTGCTG CCCTGGGCTG GGCCACTGCT 
GAGCTTATTA TGTCCCGCTG CATTCCCCTA TGGGTCGGAG CCCGGGGCAT TGAGTTTGAC 
TGGAAGTACA TCCAGATGAG CATAGACTCC AACATCAGTC TGGTCCATTA CATCGTCGCG 
TCTGCTCAGG TCTGGATGAT AACACGCTAT GATCTGTACC ACACCTTCCG GCCAGCTGTC 
CTCCTGCTGA TGTTCCTCAG TGTCTACAAG GCCTTTGTTA TGGAGACCTT CGTCCACCTC 
TGCTCGCTGG GCAGTTGGGC AGCTCTACTG GCCCGAGCAG TGGTAACGGG GCTGCTGGCC 
CTCAGCACTT TGGCCCTGTA TGTCGCCGTT GTCAATGTGC ACTCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu55834
Clone ID Related Accession (Same CDS sequence) NM_001242597.1 , NM_001242597.2
Accession Version NM_001242597.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 528bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-10
Organism Homo sapiens(human)
Product transmembrane protein 147 isoform 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM423809.1, BG697070.1, AW473288.1 and AW130399.1. On May 31, 2019 this sequence version replaced NM_001242597.1. Transcript Variant: This variant (2) lacks a splice site in the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.226436.1, SRR1163657.26227.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
ATGCTGTTCT TGGCCACTTT CTTTCCCACC TGGGAAGGCG GCATCTATGA CTTCATTGGG 
GAGTTCATGA AGGCCAGCGT GGATGTGGCA GACCTGATAG GTCTAAACCT TGTCATGTCC 
CGGAATGCCG GCAAGGGAGA GTACAAGATC ATGGTTGCTG CCCTGGGCTG GGCCACTGCT 
GAGCTTATTA TGTCCCGCTG CATTCCCCTA TGGGTCGGAG CCCGGGGCAT TGAGTTTGAC 
TGGAAGTACA TCCAGATGAG CATAGACTCC AACATCAGTC TGGTCCATTA CATCGTCGCG 
TCTGCTCAGG TCTGGATGAT AACACGCTAT GATCTGTACC ACACCTTCCG GCCAGCTGTC 
CTCCTGCTGA TGTTCCTCAG TGTCTACAAG GCCTTTGTTA TGGAGACCTT CGTCCACCTC 
TGCTCGCTGG GCAGTTGGGC AGCTCTACTG GCCCGAGCAG TGGTAACGGG GCTGCTGGCC 
CTCAGCACTT TGGCCCTGTA TGTCGCCGTT GTCAATGTGC ACTCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001229526.1
CDS333..860
Translation

Target ORF information:

RefSeq Version NM_001242597.2
Organism Homo sapiens(human)
Definition Homo sapiens transmembrane protein 147 (TMEM147), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001242597.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGCTGTTCT TGGCCACTTT CTTTCCCACC TGGGAAGGCG GCATCTATGA CTTCATTGGG 
GAGTTCATGA AGGCCAGCGT GGATGTGGCA GACCTGATAG GTCTAAACCT TGTCATGTCC 
CGGAATGCCG GCAAGGGAGA GTACAAGATC ATGGTTGCTG CCCTGGGCTG GGCCACTGCT 
GAGCTTATTA TGTCCCGCTG CATTCCCCTA TGGGTCGGAG CCCGGGGCAT TGAGTTTGAC 
TGGAAGTACA TCCAGATGAG CATAGACTCC AACATCAGTC TGGTCCATTA CATCGTCGCG 
TCTGCTCAGG TCTGGATGAT AACACGCTAT GATCTGTACC ACACCTTCCG GCCAGCTGTC 
CTCCTGCTGA TGTTCCTCAG TGTCTACAAG GCCTTTGTTA TGGAGACCTT CGTCCACCTC 
TGCTCGCTGG GCAGTTGGGC AGCTCTACTG GCCCGAGCAG TGGTAACGGG GCTGCTGGCC 
CTCAGCACTT TGGCCCTGTA TGTCGCCGTT GTCAATGTGC ACTCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu55915
Clone ID Related Accession (Same CDS sequence) NM_032635.4 , NM_032635.3
Accession Version NM_032635.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 675bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-09
Organism Homo sapiens(human)
Product transmembrane protein 147 isoform 1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM423809.1, BC001118.2 and AW130399.1. On Nov 23, 2018 this sequence version replaced NM_032635.3. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR5189658.62246.1, SRR1163655.97135.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000222284.10/ ENSP00000222284.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
ATGACCCTGT TTCACTTCGG GAACTGCTTC GCTCTTGCCT ACTTCCCCTA CTTCATCACC 
TACAAGTGCA GCGGCCTGTC CGAGTACAAC GCCTTCTGGA AATGCGTCCA GGCTGGAGTC 
ACCTACCTCT TTGTCCAACT CTGCAAGATG CTGTTCTTGG CCACTTTCTT TCCCACCTGG 
GAAGGCGGCA TCTATGACTT CATTGGGGAG TTCATGAAGG CCAGCGTGGA TGTGGCAGAC 
CTGATAGGTC TAAACCTTGT CATGTCCCGG AATGCCGGCA AGGGAGAGTA CAAGATCATG 
GTTGCTGCCC TGGGCTGGGC CACTGCTGAG CTTATTATGT CCCGCTGCAT TCCCCTATGG 
GTCGGAGCCC GGGGCATTGA GTTTGACTGG AAGTACATCC AGATGAGCAT AGACTCCAAC 
ATCAGTCTGG TCCATTACAT CGTCGCGTCT GCTCAGGTCT GGATGATAAC ACGCTATGAT 
CTGTACCACA CCTTCCGGCC AGCTGTCCTC CTGCTGATGT TCCTCAGTGT CTACAAGGCC 
TTTGTTATGG AGACCTTCGT CCACCTCTGC TCGCTGGGCA GTTGGGCAGC TCTACTGGCC 
CGAGCAGTGG TAACGGGGCT GCTGGCCCTC AGCACTTTGG CCCTGTATGT CGCCGTTGTC 
AATGTGCACT CCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_116024.1
CDS115..789
Translation

Target ORF information:

RefSeq Version NM_032635.4
Organism Homo sapiens(human)
Definition Homo sapiens transmembrane protein 147 (TMEM147), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_032635.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGACCCTGT TTCACTTCGG GAACTGCTTC GCTCTTGCCT ACTTCCCCTA CTTCATCACC 
TACAAGTGCA GCGGCCTGTC CGAGTACAAC GCCTTCTGGA AATGCGTCCA GGCTGGAGTC 
ACCTACCTCT TTGTCCAACT CTGCAAGATG CTGTTCTTGG CCACTTTCTT TCCCACCTGG 
GAAGGCGGCA TCTATGACTT CATTGGGGAG TTCATGAAGG CCAGCGTGGA TGTGGCAGAC 
CTGATAGGTC TAAACCTTGT CATGTCCCGG AATGCCGGCA AGGGAGAGTA CAAGATCATG 
GTTGCTGCCC TGGGCTGGGC CACTGCTGAG CTTATTATGT CCCGCTGCAT TCCCCTATGG 
GTCGGAGCCC GGGGCATTGA GTTTGACTGG AAGTACATCC AGATGAGCAT AGACTCCAAC 
ATCAGTCTGG TCCATTACAT CGTCGCGTCT GCTCAGGTCT GGATGATAAC ACGCTATGAT 
CTGTACCACA CCTTCCGGCC AGCTGTCCTC CTGCTGATGT TCCTCAGTGT CTACAAGGCC 
TTTGTTATGG AGACCTTCGT CCACCTCTGC TCGCTGGGCA GTTGGGCAGC TCTACTGGCC 
CGAGCAGTGG TAACGGGGCT GCTGGCCCTC AGCACTTTGG CCCTGTATGT CGCCGTTGTC 
AATGTGCACT CCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu55915
Clone ID Related Accession (Same CDS sequence) NM_032635.4 , NM_032635.3
Accession Version NM_032635.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 675bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-10-01
Organism Homo sapiens(human)
Product transmembrane protein 147 isoform 1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM423809.1, BC001118.2 and AW130399.1. On Jun 16, 2011 this sequence version replaced NM_032635.2. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1163657.269744.1, SRR1163655.444231.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
ATGACCCTGT TTCACTTCGG GAACTGCTTC GCTCTTGCCT ACTTCCCCTA CTTCATCACC 
TACAAGTGCA GCGGCCTGTC CGAGTACAAC GCCTTCTGGA AATGCGTCCA GGCTGGAGTC 
ACCTACCTCT TTGTCCAACT CTGCAAGATG CTGTTCTTGG CCACTTTCTT TCCCACCTGG 
GAAGGCGGCA TCTATGACTT CATTGGGGAG TTCATGAAGG CCAGCGTGGA TGTGGCAGAC 
CTGATAGGTC TAAACCTTGT CATGTCCCGG AATGCCGGCA AGGGAGAGTA CAAGATCATG 
GTTGCTGCCC TGGGCTGGGC CACTGCTGAG CTTATTATGT CCCGCTGCAT TCCCCTATGG 
GTCGGAGCCC GGGGCATTGA GTTTGACTGG AAGTACATCC AGATGAGCAT AGACTCCAAC 
ATCAGTCTGG TCCATTACAT CGTCGCGTCT GCTCAGGTCT GGATGATAAC ACGCTATGAT 
CTGTACCACA CCTTCCGGCC AGCTGTCCTC CTGCTGATGT TCCTCAGTGT CTACAAGGCC 
TTTGTTATGG AGACCTTCGT CCACCTCTGC TCGCTGGGCA GTTGGGCAGC TCTACTGGCC 
CGAGCAGTGG TAACGGGGCT GCTGGCCCTC AGCACTTTGG CCCTGTATGT CGCCGTTGTC 
AATGTGCACT CCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_116024.1
CDS141..815
Translation

Target ORF information:

RefSeq Version NM_032635.3
Organism Homo sapiens(human)
Definition Homo sapiens transmembrane protein 147 (TMEM147), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_032635.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGACCCTGT TTCACTTCGG GAACTGCTTC GCTCTTGCCT ACTTCCCCTA CTTCATCACC 
TACAAGTGCA GCGGCCTGTC CGAGTACAAC GCCTTCTGGA AATGCGTCCA GGCTGGAGTC 
ACCTACCTCT TTGTCCAACT CTGCAAGATG CTGTTCTTGG CCACTTTCTT TCCCACCTGG 
GAAGGCGGCA TCTATGACTT CATTGGGGAG TTCATGAAGG CCAGCGTGGA TGTGGCAGAC 
CTGATAGGTC TAAACCTTGT CATGTCCCGG AATGCCGGCA AGGGAGAGTA CAAGATCATG 
GTTGCTGCCC TGGGCTGGGC CACTGCTGAG CTTATTATGT CCCGCTGCAT TCCCCTATGG 
GTCGGAGCCC GGGGCATTGA GTTTGACTGG AAGTACATCC AGATGAGCAT AGACTCCAAC 
ATCAGTCTGG TCCATTACAT CGTCGCGTCT GCTCAGGTCT GGATGATAAC ACGCTATGAT 
CTGTACCACA CCTTCCGGCC AGCTGTCCTC CTGCTGATGT TCCTCAGTGT CTACAAGGCC 
TTTGTTATGG AGACCTTCGT CCACCTCTGC TCGCTGGGCA GTTGGGCAGC TCTACTGGCC 
CGAGCAGTGG TAACGGGGCT GCTGGCCCTC AGCACTTTGG CCCTGTATGT CGCCGTTGTC 
AATGTGCACT CCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu06928
Clone ID Related Accession (Same CDS sequence) NM_001242598.1 , NM_001242598.2
Accession Version NM_001242598.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 453bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-06-24
Organism Homo sapiens(human)
Product transmembrane protein 147 isoform 3
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM423809.1, BM765457.1 and AW130399.1. Transcript Variant: This variant (3) lacks two consecutive exons in the coding region but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BM765457.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
ATGACCCTGT TTCACTTCGG GAACTGCTTC GCTCTTGCCT ACTTCCCCTA CTTCATCACC 
TACAAGTGCA GCGGCCTGTC CGAGTACAAC GCCTTCTGGA AATGCGTCCA GGCTGGAGTC 
ACCTACCTCT TTGTCCAACT CTGCAAGATG CTGTTCTTGG CCACTTTCTT TCCCACCTGG 
GAAGGCGGCA TCTATGACTT CATTGGGGTC CATTACATCG TCGCGTCTGC TCAGGTCTGG 
ATGATAACAC GCTATGATCT GTACCACACC TTCCGGCCAG CTGTCCTCCT GCTGATGTTC 
CTCAGTGTCT ACAAGGCCTT TGTTATGGAG ACCTTCGTCC ACCTCTGCTC GCTGGGCAGT 
TGGGCAGCTC TACTGGCCCG AGCAGTGGTA ACGGGGCTGC TGGCCCTCAG CACTTTGGCC 
CTGTATGTCG CCGTTGTCAA TGTGCACTCC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001229527.1
CDS141..593
Translation

Target ORF information:

RefSeq Version NM_001242598.1
Organism Homo sapiens(human)
Definition Homo sapiens transmembrane protein 147 (TMEM147), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001242598.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGACCCTGT TTCACTTCGG GAACTGCTTC GCTCTTGCCT ACTTCCCCTA CTTCATCACC 
TACAAGTGCA GCGGCCTGTC CGAGTACAAC GCCTTCTGGA AATGCGTCCA GGCTGGAGTC 
ACCTACCTCT TTGTCCAACT CTGCAAGATG CTGTTCTTGG CCACTTTCTT TCCCACCTGG 
GAAGGCGGCA TCTATGACTT CATTGGGGTC CATTACATCG TCGCGTCTGC TCAGGTCTGG 
ATGATAACAC GCTATGATCT GTACCACACC TTCCGGCCAG CTGTCCTCCT GCTGATGTTC 
CTCAGTGTCT ACAAGGCCTT TGTTATGGAG ACCTTCGTCC ACCTCTGCTC GCTGGGCAGT 
TGGGCAGCTC TACTGGCCCG AGCAGTGGTA ACGGGGCTGC TGGCCCTCAG CACTTTGGCC 
CTGTATGTCG CCGTTGTCAA TGTGCACTCC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu06928
Clone ID Related Accession (Same CDS sequence) NM_001242598.1 , NM_001242598.2
Accession Version NM_001242598.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 453bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-10
Organism Homo sapiens(human)
Product transmembrane protein 147 isoform 3
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM423809.1, BM765457.1 and AW130399.1. On May 31, 2019 this sequence version replaced NM_001242598.1. Transcript Variant: This variant (3) lacks two consecutive exons in the coding region but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BM765457.1, DRR138512.395010.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
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421
ATGACCCTGT TTCACTTCGG GAACTGCTTC GCTCTTGCCT ACTTCCCCTA CTTCATCACC 
TACAAGTGCA GCGGCCTGTC CGAGTACAAC GCCTTCTGGA AATGCGTCCA GGCTGGAGTC 
ACCTACCTCT TTGTCCAACT CTGCAAGATG CTGTTCTTGG CCACTTTCTT TCCCACCTGG 
GAAGGCGGCA TCTATGACTT CATTGGGGTC CATTACATCG TCGCGTCTGC TCAGGTCTGG 
ATGATAACAC GCTATGATCT GTACCACACC TTCCGGCCAG CTGTCCTCCT GCTGATGTTC 
CTCAGTGTCT ACAAGGCCTT TGTTATGGAG ACCTTCGTCC ACCTCTGCTC GCTGGGCAGT 
TGGGCAGCTC TACTGGCCCG AGCAGTGGTA ACGGGGCTGC TGGCCCTCAG CACTTTGGCC 
CTGTATGTCG CCGTTGTCAA TGTGCACTCC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001229527.1
CDS115..567
Translation

Target ORF information:

RefSeq Version NM_001242598.2
Organism Homo sapiens(human)
Definition Homo sapiens transmembrane protein 147 (TMEM147), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001242598.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGACCCTGT TTCACTTCGG GAACTGCTTC GCTCTTGCCT ACTTCCCCTA CTTCATCACC 
TACAAGTGCA GCGGCCTGTC CGAGTACAAC GCCTTCTGGA AATGCGTCCA GGCTGGAGTC 
ACCTACCTCT TTGTCCAACT CTGCAAGATG CTGTTCTTGG CCACTTTCTT TCCCACCTGG 
GAAGGCGGCA TCTATGACTT CATTGGGGTC CATTACATCG TCGCGTCTGC TCAGGTCTGG 
ATGATAACAC GCTATGATCT GTACCACACC TTCCGGCCAG CTGTCCTCCT GCTGATGTTC 
CTCAGTGTCT ACAAGGCCTT TGTTATGGAG ACCTTCGTCC ACCTCTGCTC GCTGGGCAGT 
TGGGCAGCTC TACTGGCCCG AGCAGTGGTA ACGGGGCTGC TGGCCCTCAG CACTTTGGCC 
CTGTATGTCG CCGTTGTCAA TGTGCACTCC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW


book

Transmembrane protein 147 (TMEM147) is a novel component of the Nicalin-NOMO protein complex.
The Journal of biological chemistry285(34)26174-81(2010 Aug)
Dettmer U,Kuhn PH,Abou-Ajram C,Lichtenthaler SF,Kr?ger M,Kremmer E,Haass C,Haffner C


book

Transcriptome analysis of human gastric cancer.
Mammalian genome : official journal of the International Mammalian Genome Society16(12)942-54(2005 Dec)
Oh JH,Yang JO,Hahn Y,Kim MR,Byun SS,Jeon YJ,Kim JM,Song KS,Noh SM,Kim S,Yoo HS,Kim YS,Kim NS


book

Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region.
Genomics83(1)153-67(2004 Jan)
Lehner B,Semple JI,Brown SE,Counsell D,Campbell RD,Sanderson CM