PITRM1 cDNA ORF clone, Homo sapiens(human)

The following PITRM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PITRM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu01692 NM_001242309.1
Latest version!
Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu101458 NM_001347726.2
Latest version!
Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $391.30
$559.00
OHu43656 NM_001347725.2
Latest version!
Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu101478 NM_001347730.1
Latest version!
Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu101521 NM_001347727.2
Latest version!
Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu101524 NM_001347728.2
Latest version!
Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu101531 NM_001347729.1
Latest version!
Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu01770 NM_001242307.1
Latest version!
Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu01814 NM_014889.4
Latest version!
Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $384.30
$549.00
View Old Accession Versions >>
OHu101521 NM_001347727.1 Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu01814 NM_014889.3 Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $384.30
$549.00
OHu101458 NM_001347726.1 Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $391.30
$559.00
OHu101524 NM_001347728.1 Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu43656 NM_001347725.1 Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu01692
    Clone ID Related Accession (Same CDS sequence) NM_001242309.1
    Accession Version NM_001242309.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2820bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-21
    Organism Homo sapiens(human)
    Product presequence protease, mitochondrial isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA331909.1, AK002061.1, AK303406.1, AL451164.13 and BC095422.1. Transcript Variant: This variant (3) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an alternate AUG and results an isoform (3) with a distinct N-terminus, compared to isoform 1. The encoded protein likely does not localize to the mitochondrion. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK303406.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCGCAATG TGGCGCTGCG GCGGGCGGCA GGGCCTGTGT GTGCTGAGGC GGCTGAGCGG 
    CGGGTGACAT CTGTTCCCGA GCTGTTCCTG ACTGCAGTGA AGCTCACCCA TGATGACACA
    GGAGCCAGGT ATTTACACCT GGCCAGAGAA GACACGAATA ATCTGTTCAG CGTGCAGTTC
    CGTACTACTC CCATGGACAG TACTGGTGTT CCTCACATTC TTGAGCATAC CGTCCTTTGT
    GGGTCTCAGA AATATCCGTG CAGAGACCCT TTCTTCAAAA TGTTGAACCG GTCCCTCTCC
    ACGTTCATGA ACGCCTTCAC AGCTAGTGAT TATACTCTGT ATCCATTTTC CACACAAAAT
    CCCAAGGACT TTCAGAATCT CCTCTCGGTG TATTTGGATG CCACCTTTTT CCCATGTTTA
    CGCGAGCTGG ATTTCTGGCA GGAAGGATGG CGGCTGGAAC ATGAGAATCC GAGCGACCCC
    CAGACGCCCT TGGTCTTTAA AGGAGTCGTC TTTAATGAGA TGAAGGGAGC GTTTACAGAC
    AATGAGAGGA TATTCTCCCA GCACCTTCAG AACAGACTTC TTCCTGACCA CACGTACTCA
    GTGGTCTCCG GGGGTGACCC ACTGTGCATC CCGGAGCTTA CATGGGAGCA GCTTAAGCAG
    TTTCATGCCA CTCACTATCA CCCAAGCAAT GCTAGGTTCT TCACGTACGG TAATTTTCCA
    TTAGAACAGC ATCTGAAACA AATTCACGAG GAAGCACTGA GCAAATTCCA GAAAATTGAA
    CCAAGCACCG TGGTGCCAGC TCAGACACCC TGGGACAAGC CTAGGGAATT CCAGATAACA
    TGTGGCCCGG ATTCATTTGC TACAGATCCC TCTAAACAAA CAACCATCAG CGTTAGCTTC
    CTCTTACCGG ACATCACCGA CACATTTGAA GCCTTCACAT TAAGTCTTCT GTCTTCACTC
    TTGACTTCTG GGCCCAATTC TCCCTTTTAC AAAGCCTTGA TTGAATCTGG CCTTGGCACA
    GACTTTTCTC CTGATGTTGG ATATAATGGC TACACGAGGG AGGCCTACTT TAGTGTCGGC
    CTCCAAGGGA TTGCGGAGAA AGACATTGAG ACCGTCAGAA GCCTCATAGA CAGAACGATT
    GATGAAGTAG TTGAGAAAGG ATTTGAAGAT GATCGAATTG AGGCTTTACT TCATAAAATT
    GAAATACAGA TGAAACATCA GTCTACCAGC TTTGGGCTGA TGCTGACATC ATACATAGCT
    TCTTGCTGGA ACCATGATGG GGACCCTGTG GAGCTCTTGA AGTTGGGAAA TCAGTTAGCT
    AAATTCAGAC AGTGCCTGCA GGAAAATCCA AAATTTTTGC AAGAAAAAGT AAAACAGTAT
    TTTAAGAATA ACCAGCATAA GCTGACTTTA TCGATGAGGC CAGATGACAA GTATCACGAG
    AAGCAGGCAC AGGTGGAAGC CACGAAGCTC AAGCAGAAGG TCGAGGCTCT GTCCCCCGGA
    GACAGGCAGC AGATCTACGA GAAAGGTCTA GAATTACGGA GTCAACAAAG CAAACCTCAA
    GATGCCTCTT GTCTGCCAGC GTTGAAAGTT TCCGATATTG AACCCACCAT ACCTGTCACA
    GAGTTGGACG TGGTCCTGAC AGCTGGAGAT ATCCCTGTTC AGTACTGCGC CCAGCCCACC
    AATGGCATGG TGTATTTCCG GGCCTTCTCC AGCCTGAACA CACTCCCCGA GGAGCTGAGG
    CCCTATGTGC CCCTCTTCTG CAGCGTCCTC ACCAACCCGT GCTTTGAAGA AGAGGAGCAC
    TTCAAGGTGC TGGTGAAGAT GACCGCCCAG GAGCTCGCCA ATGGAATTCC TGACTCTGGG
    CACCTGTACG CATCCATCAG GGCAGGCCGG ACCCTCACGC CCGCAGGGGA CCTGCAGGAG
    ACCTTCAGCG GGATGGATCA GGTGCGGCTG ATGAAGAGGA TTGCAGAAAT GACAGATATC
    AAACCCATCC TGAGGAAGCT CCCGCGTATC AAGAAACACT TGTTAAATGG TGATAATATG
    AGGTGTTCAG TGAATGCGAC TCCTCAGCAG ATGCCTCAGA CAGAAAAAGC GGTCGAAGAC
    TTCCTTAGAA GCATCGGTCG GAGTAAAAAG GAACGGAGGC CTGTGCGCCC ACACACGGTC
    GAGAAACCTG TGCCCAGCAG CTCTGGTGGA GATGCCCACG TTCCCCATGG CTCCCAGGTC
    ATTAGGAAGC TGGTCATGGA ACCCACCTTC AAGCCCTGGC AGATGAAGAC TCACTTCCTG
    ATGCCCTTCC CGGTGAATTA CGTGGGTGAA TGCATCCGAA CTGTCCCCTA CACGGACCCA
    GATCATGCCA GTCTTAAAAT CCTTGCACGT TTGATGACTG CCAAATTCTT GCATACAGAA
    ATTCGAGAAA AAGGCGGTGC TTATGGTGGA GGCGCAAAAC TCAGCCACAA TGGGATTTTC
    ACCCTTTACT CTTACAGGGA CCCAAATACA ATAGAGACGC TCCAGTCTTT TGGGAAGGCT
    GTCGACTGGG CTAAGTCTGG AAAATTCACA CAGCAAGACA TCGACGAAGC CAAACTTTCT
    GTCTTCTCAA CCGTAGATGC TCCTGTCGCT CCTTCAGACA AAGGAATGGA CCACTTCTTG
    TACGGCCTCT CGGATGAGAT GAAGCAGGCC CACAGAGAGC AGCTCTTTGC TGTCAGCCAC
    GACAAGCTCC TGGCCGTGAG CGATAGATAC CTCGGCACTG GGAAGAGCAC ACACGGCCTG
    GCCATCCTCG GACCCGAGAA CCCGAAAATT GCCAAGGACC CATCCTGGAT CATCCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001229238.1
    CDS63..2882
    Translation

    Target ORF information:

    RefSeq Version NM_001242309.1
    Organism Homo sapiens(human)
    Definition Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001242309.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCGCAATG TGGCGCTGCG GCGGGCGGCA GGGCCTGTGT GTGCTGAGGC GGCTGAGCGG 
    CGGGTGACAT CTGTTCCCGA GCTGTTCCTG ACTGCAGTGA AGCTCACCCA TGATGACACA
    GGAGCCAGGT ATTTACACCT GGCCAGAGAA GACACGAATA ATCTGTTCAG CGTGCAGTTC
    CGTACTACTC CCATGGACAG TACTGGTGTT CCTCACATTC TTGAGCATAC CGTCCTTTGT
    GGGTCTCAGA AATATCCGTG CAGAGACCCT TTCTTCAAAA TGTTGAACCG GTCCCTCTCC
    ACGTTCATGA ACGCCTTCAC AGCTAGTGAT TATACTCTGT ATCCATTTTC CACACAAAAT
    CCCAAGGACT TTCAGAATCT CCTCTCGGTG TATTTGGATG CCACCTTTTT CCCATGTTTA
    CGCGAGCTGG ATTTCTGGCA GGAAGGATGG CGGCTGGAAC ATGAGAATCC GAGCGACCCC
    CAGACGCCCT TGGTCTTTAA AGGAGTCGTC TTTAATGAGA TGAAGGGAGC GTTTACAGAC
    AATGAGAGGA TATTCTCCCA GCACCTTCAG AACAGACTTC TTCCTGACCA CACGTACTCA
    GTGGTCTCCG GGGGTGACCC ACTGTGCATC CCGGAGCTTA CATGGGAGCA GCTTAAGCAG
    TTTCATGCCA CTCACTATCA CCCAAGCAAT GCTAGGTTCT TCACGTACGG TAATTTTCCA
    TTAGAACAGC ATCTGAAACA AATTCACGAG GAAGCACTGA GCAAATTCCA GAAAATTGAA
    CCAAGCACCG TGGTGCCAGC TCAGACACCC TGGGACAAGC CTAGGGAATT CCAGATAACA
    TGTGGCCCGG ATTCATTTGC TACAGATCCC TCTAAACAAA CAACCATCAG CGTTAGCTTC
    CTCTTACCGG ACATCACCGA CACATTTGAA GCCTTCACAT TAAGTCTTCT GTCTTCACTC
    TTGACTTCTG GGCCCAATTC TCCCTTTTAC AAAGCCTTGA TTGAATCTGG CCTTGGCACA
    GACTTTTCTC CTGATGTTGG ATATAATGGC TACACGAGGG AGGCCTACTT TAGTGTCGGC
    CTCCAAGGGA TTGCGGAGAA AGACATTGAG ACCGTCAGAA GCCTCATAGA CAGAACGATT
    GATGAAGTAG TTGAGAAAGG ATTTGAAGAT GATCGAATTG AGGCTTTACT TCATAAAATT
    GAAATACAGA TGAAACATCA GTCTACCAGC TTTGGGCTGA TGCTGACATC ATACATAGCT
    TCTTGCTGGA ACCATGATGG GGACCCTGTG GAGCTCTTGA AGTTGGGAAA TCAGTTAGCT
    AAATTCAGAC AGTGCCTGCA GGAAAATCCA AAATTTTTGC AAGAAAAAGT AAAACAGTAT
    TTTAAGAATA ACCAGCATAA GCTGACTTTA TCGATGAGGC CAGATGACAA GTATCACGAG
    AAGCAGGCAC AGGTGGAAGC CACGAAGCTC AAGCAGAAGG TCGAGGCTCT GTCCCCCGGA
    GACAGGCAGC AGATCTACGA GAAAGGTCTA GAATTACGGA GTCAACAAAG CAAACCTCAA
    GATGCCTCTT GTCTGCCAGC GTTGAAAGTT TCCGATATTG AACCCACCAT ACCTGTCACA
    GAGTTGGACG TGGTCCTGAC AGCTGGAGAT ATCCCTGTTC AGTACTGCGC CCAGCCCACC
    AATGGCATGG TGTATTTCCG GGCCTTCTCC AGCCTGAACA CACTCCCCGA GGAGCTGAGG
    CCCTATGTGC CCCTCTTCTG CAGCGTCCTC ACCAACCCGT GCTTTGAAGA AGAGGAGCAC
    TTCAAGGTGC TGGTGAAGAT GACCGCCCAG GAGCTCGCCA ATGGAATTCC TGACTCTGGG
    CACCTGTACG CATCCATCAG GGCAGGCCGG ACCCTCACGC CCGCAGGGGA CCTGCAGGAG
    ACCTTCAGCG GGATGGATCA GGTGCGGCTG ATGAAGAGGA TTGCAGAAAT GACAGATATC
    AAACCCATCC TGAGGAAGCT CCCGCGTATC AAGAAACACT TGTTAAATGG TGATAATATG
    AGGTGTTCAG TGAATGCGAC TCCTCAGCAG ATGCCTCAGA CAGAAAAAGC GGTCGAAGAC
    TTCCTTAGAA GCATCGGTCG GAGTAAAAAG GAACGGAGGC CTGTGCGCCC ACACACGGTC
    GAGAAACCTG TGCCCAGCAG CTCTGGTGGA GATGCCCACG TTCCCCATGG CTCCCAGGTC
    ATTAGGAAGC TGGTCATGGA ACCCACCTTC AAGCCCTGGC AGATGAAGAC TCACTTCCTG
    ATGCCCTTCC CGGTGAATTA CGTGGGTGAA TGCATCCGAA CTGTCCCCTA CACGGACCCA
    GATCATGCCA GTCTTAAAAT CCTTGCACGT TTGATGACTG CCAAATTCTT GCATACAGAA
    ATTCGAGAAA AAGGCGGTGC TTATGGTGGA GGCGCAAAAC TCAGCCACAA TGGGATTTTC
    ACCCTTTACT CTTACAGGGA CCCAAATACA ATAGAGACGC TCCAGTCTTT TGGGAAGGCT
    GTCGACTGGG CTAAGTCTGG AAAATTCACA CAGCAAGACA TCGACGAAGC CAAACTTTCT
    GTCTTCTCAA CCGTAGATGC TCCTGTCGCT CCTTCAGACA AAGGAATGGA CCACTTCTTG
    TACGGCCTCT CGGATGAGAT GAAGCAGGCC CACAGAGAGC AGCTCTTTGC TGTCAGCCAC
    GACAAGCTCC TGGCCGTGAG CGATAGATAC CTCGGCACTG GGAAGAGCAC ACACGGCCTG
    GCCATCCTCG GACCCGAGAA CCCGAAAATT GCCAAGGACC CATCCTGGAT CATCCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101458
    Clone ID Related Accession (Same CDS sequence) NM_001347726.1 , NM_001347726.2
    Accession Version NM_001347726.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product presequence protease, mitochondrial isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL451164.13. On Dec 13, 2016 this sequence version replaced XM_017015473.1. Transcript Variant: This variant (5) encodes isoform 5, which likely does not localize to the mitochondrion. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.282095.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAAGGGAG CGTTTACAGA CAATGAGAGG ATATTCTCCC AGCACCTTCA GAACAGACTT 
    CTTCCTGACC ACACGTACTC AGTGGTCTCC GGGGGTGACC CACTGTGCAT CCCGGAGCTT
    ACATGGGAGC AGCTTAAGCA GTTTCATGCC ACTCACTATC ACCCAAGCAA TGCTAGGTTC
    TTCACGTACG GTAATTTTCC ATTAGAACAG CATCTGAAAC AAATTCACGA GGAAGCACTG
    AGCAAATTCC AGAAAATTGA ACCAAGCACC GTGGTGCCAG CTCAGACACC CTGGGACAAG
    CCTAGGGAAT TCCAGATAAC ATGTGGCCCG GATTCATTTG CTACAGATCC CTCTAAACAA
    ACAACCATCA GCGTTAGCTT CCTCTTACCG GACATCACCG ACACATTTGA AGCCTTCACA
    TTAAGTCTTC TGTCTTCACT CTTGACTTCT GGGCCCAATT CTCCCTTTTA CAAAGCCTTG
    ATTGAATCTG GCCTTGGCAC AGACTTTTCT CCTGATGTTG GATATAATGG CTACACGAGG
    GAGGCCTACT TTAGTGTCGG CCTCCAAGGG ATTGCGGAGA AAGACATTGA GACCGTCAGA
    AGCCTCATAG ACAGAACGAT TGATGAAGTA GTTGAGAAAG GATTTGAAGA TGATCGAATT
    GAGGCTTTAC TTCATAAAAT TGAAATACAG ATGAAACATC AGTCTACCAG CTTTGGGCTG
    ATGCTGACAT CATACATAGC TTCTTGCTGG AACCATGATG GGGACCCTGT GGAGCTCTTG
    AAGTTGGGAA ATCAGTTAGC TAAATTCAGA CAGTGCCTGC AGGAAAATCC AAAATTTTTG
    CAAGAAAAAG TAAAACAGTA TTTTAAGAAT AACCAGCATA AGCTGACTTT ATCGATGAGG
    CCAGATGACA AGTATCACGA GAAGCAGGCA CAGGTGGAAG CCACGAAGCT CAAGCAGAAG
    GTCGAGGCTC TGTCCCCCGG AGACAGGCAG CAGATCTACG AGAAAGGTCT AGAATTACGG
    AGTCAACAAA GCAAACCTCA AGATGCCTCT TGTCTGCCAG CGTTGAAAGT TTCCGATATT
    GAACCCACCA TACCTGTCAC AGAGTTGGAC GTGGTCCTGA CAGCTGGAGA TATCCCTGTT
    CAGTACTGCG CCCAGCCCAC CAATGGCATG GTGTATTTCC GGGCCTTCTC CAGCCTGAAC
    ACACTCCCCG AGGAGCTGAG GCCCTATGTG CCCCTCTTCT GCAGCGTCCT CACCAACCCG
    TGCTTTGAAG AAGAGGAGCA CTTCAAGGTG CTGGTGAAGA TGACCGCCCA GGAGCTCGCC
    AATGGAATTC CTGACTCTGG GCACCTGTAC GCATCCATCA GGGCAGGCCG GACCCTCACG
    CCCGCAGGGG ACCTGCAGGA GACCTTCAGC GGGATGGATC AGGTGCGGCT GATGAAGAGG
    ATTGCAGAAA TGACAGATAT CAAACCCATC CTGAGGAAGC TCCCGCGTAT CAAGAAACAC
    TTGTTAAATG GTGATAATAT GAGGTGTTCA GTGAATGCGA CTCCTCAGCA GATGCCTCAG
    ACAGAAAAAG CGGTCGAAGA CTTCCTTAGA AGCATCGGTC GGAGTAAAAA GGAACGGAGG
    CCTGTGCGCC CACACACGGT CGAGAAACCT GTGCCCAGCA GCTCTGGTGG AGATGCCCAC
    GTTCCCCATG GCTCCCAGGT CATTAGGAAG CTGGTCATGG AACCCACCTT CAAGCCCTGG
    CAGATGAAGA CTCACTTCCT GATGCCCTTC CCGGTGAATT ACGTGGGTGA ATGCATCCGA
    ACTGTCCCCT ACACGGACCC AGATCATGCC AGTCTTAAAA TCCTTGCACG TTTGATGACT
    GCCAAATTCT TGCATACAGA AATTCGAGAA AAAGGCGGTG CTTATGGTGG AGGCGCAAAA
    CTCAGCCACA ATGGGATTTT CACCCTTTAC TCTTACAGGG ACCCAAATAC AATAGAGACG
    CTCCAGTCTT TTGGGAAGGC TGTCGACTGG GCTAAGTCTG GAAAATTCAC ACAGCAAGAC
    ATCGACGAAG CCAAACTTTC TGTCTTCTCA ACCGTAGATG CTCCTGTCGC TCCTTCAGAC
    AAAGGAATGG ACCACTTCTT GTACGGCCTC TCGGATGAGA TGAAGCAGGC CCACAGAGAG
    CAGCTCTTTG CTGTCAGCCA CGACAAGCTC CTGGCCGTGA GCGATAGATA CCTCGGCACT
    GGGAAGAGCA CACACGGCCT GGCCATCCTC GGACCCGAGA ACCCGAAAAT TGCCAAGGAC
    CCATCCTGGA TCATCCAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334655.1
    CDS641..2941
    Translation

    Target ORF information:

    RefSeq Version NM_001347726.1
    Organism Homo sapiens(human)
    Definition Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347726.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAAGGGAG CGTTTACAGA CAATGAGAGG ATATTCTCCC AGCACCTTCA GAACAGACTT 
    CTTCCTGACC ACACGTACTC AGTGGTCTCC GGGGGTGACC CACTGTGCAT CCCGGAGCTT
    ACATGGGAGC AGCTTAAGCA GTTTCATGCC ACTCACTATC ACCCAAGCAA TGCTAGGTTC
    TTCACGTACG GTAATTTTCC ATTAGAACAG CATCTGAAAC AAATTCACGA GGAAGCACTG
    AGCAAATTCC AGAAAATTGA ACCAAGCACC GTGGTGCCAG CTCAGACACC CTGGGACAAG
    CCTAGGGAAT TCCAGATAAC ATGTGGCCCG GATTCATTTG CTACAGATCC CTCTAAACAA
    ACAACCATCA GCGTTAGCTT CCTCTTACCG GACATCACCG ACACATTTGA AGCCTTCACA
    TTAAGTCTTC TGTCTTCACT CTTGACTTCT GGGCCCAATT CTCCCTTTTA CAAAGCCTTG
    ATTGAATCTG GCCTTGGCAC AGACTTTTCT CCTGATGTTG GATATAATGG CTACACGAGG
    GAGGCCTACT TTAGTGTCGG CCTCCAAGGG ATTGCGGAGA AAGACATTGA GACCGTCAGA
    AGCCTCATAG ACAGAACGAT TGATGAAGTA GTTGAGAAAG GATTTGAAGA TGATCGAATT
    GAGGCTTTAC TTCATAAAAT TGAAATACAG ATGAAACATC AGTCTACCAG CTTTGGGCTG
    ATGCTGACAT CATACATAGC TTCTTGCTGG AACCATGATG GGGACCCTGT GGAGCTCTTG
    AAGTTGGGAA ATCAGTTAGC TAAATTCAGA CAGTGCCTGC AGGAAAATCC AAAATTTTTG
    CAAGAAAAAG TAAAACAGTA TTTTAAGAAT AACCAGCATA AGCTGACTTT ATCGATGAGG
    CCAGATGACA AGTATCACGA GAAGCAGGCA CAGGTGGAAG CCACGAAGCT CAAGCAGAAG
    GTCGAGGCTC TGTCCCCCGG AGACAGGCAG CAGATCTACG AGAAAGGTCT AGAATTACGG
    AGTCAACAAA GCAAACCTCA AGATGCCTCT TGTCTGCCAG CGTTGAAAGT TTCCGATATT
    GAACCCACCA TACCTGTCAC AGAGTTGGAC GTGGTCCTGA CAGCTGGAGA TATCCCTGTT
    CAGTACTGCG CCCAGCCCAC CAATGGCATG GTGTATTTCC GGGCCTTCTC CAGCCTGAAC
    ACACTCCCCG AGGAGCTGAG GCCCTATGTG CCCCTCTTCT GCAGCGTCCT CACCAACCCG
    TGCTTTGAAG AAGAGGAGCA CTTCAAGGTG CTGGTGAAGA TGACCGCCCA GGAGCTCGCC
    AATGGAATTC CTGACTCTGG GCACCTGTAC GCATCCATCA GGGCAGGCCG GACCCTCACG
    CCCGCAGGGG ACCTGCAGGA GACCTTCAGC GGGATGGATC AGGTGCGGCT GATGAAGAGG
    ATTGCAGAAA TGACAGATAT CAAACCCATC CTGAGGAAGC TCCCGCGTAT CAAGAAACAC
    TTGTTAAATG GTGATAATAT GAGGTGTTCA GTGAATGCGA CTCCTCAGCA GATGCCTCAG
    ACAGAAAAAG CGGTCGAAGA CTTCCTTAGA AGCATCGGTC GGAGTAAAAA GGAACGGAGG
    CCTGTGCGCC CACACACGGT CGAGAAACCT GTGCCCAGCA GCTCTGGTGG AGATGCCCAC
    GTTCCCCATG GCTCCCAGGT CATTAGGAAG CTGGTCATGG AACCCACCTT CAAGCCCTGG
    CAGATGAAGA CTCACTTCCT GATGCCCTTC CCGGTGAATT ACGTGGGTGA ATGCATCCGA
    ACTGTCCCCT ACACGGACCC AGATCATGCC AGTCTTAAAA TCCTTGCACG TTTGATGACT
    GCCAAATTCT TGCATACAGA AATTCGAGAA AAAGGCGGTG CTTATGGTGG AGGCGCAAAA
    CTCAGCCACA ATGGGATTTT CACCCTTTAC TCTTACAGGG ACCCAAATAC AATAGAGACG
    CTCCAGTCTT TTGGGAAGGC TGTCGACTGG GCTAAGTCTG GAAAATTCAC ACAGCAAGAC
    ATCGACGAAG CCAAACTTTC TGTCTTCTCA ACCGTAGATG CTCCTGTCGC TCCTTCAGAC
    AAAGGAATGG ACCACTTCTT GTACGGCCTC TCGGATGAGA TGAAGCAGGC CCACAGAGAG
    CAGCTCTTTG CTGTCAGCCA CGACAAGCTC CTGGCCGTGA GCGATAGATA CCTCGGCACT
    GGGAAGAGCA CACACGGCCT GGCCATCCTC GGACCCGAGA ACCCGAAAAT TGCCAAGGAC
    CCATCCTGGA TCATCCAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101458
    Clone ID Related Accession (Same CDS sequence) NM_001347726.1 , NM_001347726.2
    Accession Version NM_001347726.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-25
    Organism Homo sapiens(human)
    Product presequence protease, mitochondrial isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL451164.13. On May 31, 2019 this sequence version replaced NM_001347726.1. Transcript Variant: This variant (5) encodes isoform 5, which likely does not localize to the mitochondrion. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.282095.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAAGGGAG CGTTTACAGA CAATGAGAGG ATATTCTCCC AGCACCTTCA GAACAGACTT 
    CTTCCTGACC ACACGTACTC AGTGGTCTCC GGGGGTGACC CACTGTGCAT CCCGGAGCTT
    ACATGGGAGC AGCTTAAGCA GTTTCATGCC ACTCACTATC ACCCAAGCAA TGCTAGGTTC
    TTCACGTACG GTAATTTTCC ATTAGAACAG CATCTGAAAC AAATTCACGA GGAAGCACTG
    AGCAAATTCC AGAAAATTGA ACCAAGCACC GTGGTGCCAG CTCAGACACC CTGGGACAAG
    CCTAGGGAAT TCCAGATAAC ATGTGGCCCG GATTCATTTG CTACAGATCC CTCTAAACAA
    ACAACCATCA GCGTTAGCTT CCTCTTACCG GACATCACCG ACACATTTGA AGCCTTCACA
    TTAAGTCTTC TGTCTTCACT CTTGACTTCT GGGCCCAATT CTCCCTTTTA CAAAGCCTTG
    ATTGAATCTG GCCTTGGCAC AGACTTTTCT CCTGATGTTG GATATAATGG CTACACGAGG
    GAGGCCTACT TTAGTGTCGG CCTCCAAGGG ATTGCGGAGA AAGACATTGA GACCGTCAGA
    AGCCTCATAG ACAGAACGAT TGATGAAGTA GTTGAGAAAG GATTTGAAGA TGATCGAATT
    GAGGCTTTAC TTCATAAAAT TGAAATACAG ATGAAACATC AGTCTACCAG CTTTGGGCTG
    ATGCTGACAT CATACATAGC TTCTTGCTGG AACCATGATG GGGACCCTGT GGAGCTCTTG
    AAGTTGGGAA ATCAGTTAGC TAAATTCAGA CAGTGCCTGC AGGAAAATCC AAAATTTTTG
    CAAGAAAAAG TAAAACAGTA TTTTAAGAAT AACCAGCATA AGCTGACTTT ATCGATGAGG
    CCAGATGACA AGTATCACGA GAAGCAGGCA CAGGTGGAAG CCACGAAGCT CAAGCAGAAG
    GTCGAGGCTC TGTCCCCCGG AGACAGGCAG CAGATCTACG AGAAAGGTCT AGAATTACGG
    AGTCAACAAA GCAAACCTCA AGATGCCTCT TGTCTGCCAG CGTTGAAAGT TTCCGATATT
    GAACCCACCA TACCTGTCAC AGAGTTGGAC GTGGTCCTGA CAGCTGGAGA TATCCCTGTT
    CAGTACTGCG CCCAGCCCAC CAATGGCATG GTGTATTTCC GGGCCTTCTC CAGCCTGAAC
    ACACTCCCCG AGGAGCTGAG GCCCTATGTG CCCCTCTTCT GCAGCGTCCT CACCAACCCG
    TGCTTTGAAG AAGAGGAGCA CTTCAAGGTG CTGGTGAAGA TGACCGCCCA GGAGCTCGCC
    AATGGAATTC CTGACTCTGG GCACCTGTAC GCATCCATCA GGGCAGGCCG GACCCTCACG
    CCCGCAGGGG ACCTGCAGGA GACCTTCAGC GGGATGGATC AGGTGCGGCT GATGAAGAGG
    ATTGCAGAAA TGACAGATAT CAAACCCATC CTGAGGAAGC TCCCGCGTAT CAAGAAACAC
    TTGTTAAATG GTGATAATAT GAGGTGTTCA GTGAATGCGA CTCCTCAGCA GATGCCTCAG
    ACAGAAAAAG CGGTCGAAGA CTTCCTTAGA AGCATCGGTC GGAGTAAAAA GGAACGGAGG
    CCTGTGCGCC CACACACGGT CGAGAAACCT GTGCCCAGCA GCTCTGGTGG AGATGCCCAC
    GTTCCCCATG GCTCCCAGGT CATTAGGAAG CTGGTCATGG AACCCACCTT CAAGCCCTGG
    CAGATGAAGA CTCACTTCCT GATGCCCTTC CCGGTGAATT ACGTGGGTGA ATGCATCCGA
    ACTGTCCCCT ACACGGACCC AGATCATGCC AGTCTTAAAA TCCTTGCACG TTTGATGACT
    GCCAAATTCT TGCATACAGA AATTCGAGAA AAAGGCGGTG CTTATGGTGG AGGCGCAAAA
    CTCAGCCACA ATGGGATTTT CACCCTTTAC TCTTACAGGG ACCCAAATAC AATAGAGACG
    CTCCAGTCTT TTGGGAAGGC TGTCGACTGG GCTAAGTCTG GAAAATTCAC ACAGCAAGAC
    ATCGACGAAG CCAAACTTTC TGTCTTCTCA ACCGTAGATG CTCCTGTCGC TCCTTCAGAC
    AAAGGAATGG ACCACTTCTT GTACGGCCTC TCGGATGAGA TGAAGCAGGC CCACAGAGAG
    CAGCTCTTTG CTGTCAGCCA CGACAAGCTC CTGGCCGTGA GCGATAGATA CCTCGGCACT
    GGGAAGAGCA CACACGGCCT GGCCATCCTC GGACCCGAGA ACCCGAAAAT TGCCAAGGAC
    CCATCCTGGA TCATCCAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334655.1
    CDS582..2882
    Translation

    Target ORF information:

    RefSeq Version NM_001347726.2
    Organism Homo sapiens(human)
    Definition Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347726.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAAGGGAG CGTTTACAGA CAATGAGAGG ATATTCTCCC AGCACCTTCA GAACAGACTT 
    CTTCCTGACC ACACGTACTC AGTGGTCTCC GGGGGTGACC CACTGTGCAT CCCGGAGCTT
    ACATGGGAGC AGCTTAAGCA GTTTCATGCC ACTCACTATC ACCCAAGCAA TGCTAGGTTC
    TTCACGTACG GTAATTTTCC ATTAGAACAG CATCTGAAAC AAATTCACGA GGAAGCACTG
    AGCAAATTCC AGAAAATTGA ACCAAGCACC GTGGTGCCAG CTCAGACACC CTGGGACAAG
    CCTAGGGAAT TCCAGATAAC ATGTGGCCCG GATTCATTTG CTACAGATCC CTCTAAACAA
    ACAACCATCA GCGTTAGCTT CCTCTTACCG GACATCACCG ACACATTTGA AGCCTTCACA
    TTAAGTCTTC TGTCTTCACT CTTGACTTCT GGGCCCAATT CTCCCTTTTA CAAAGCCTTG
    ATTGAATCTG GCCTTGGCAC AGACTTTTCT CCTGATGTTG GATATAATGG CTACACGAGG
    GAGGCCTACT TTAGTGTCGG CCTCCAAGGG ATTGCGGAGA AAGACATTGA GACCGTCAGA
    AGCCTCATAG ACAGAACGAT TGATGAAGTA GTTGAGAAAG GATTTGAAGA TGATCGAATT
    GAGGCTTTAC TTCATAAAAT TGAAATACAG ATGAAACATC AGTCTACCAG CTTTGGGCTG
    ATGCTGACAT CATACATAGC TTCTTGCTGG AACCATGATG GGGACCCTGT GGAGCTCTTG
    AAGTTGGGAA ATCAGTTAGC TAAATTCAGA CAGTGCCTGC AGGAAAATCC AAAATTTTTG
    CAAGAAAAAG TAAAACAGTA TTTTAAGAAT AACCAGCATA AGCTGACTTT ATCGATGAGG
    CCAGATGACA AGTATCACGA GAAGCAGGCA CAGGTGGAAG CCACGAAGCT CAAGCAGAAG
    GTCGAGGCTC TGTCCCCCGG AGACAGGCAG CAGATCTACG AGAAAGGTCT AGAATTACGG
    AGTCAACAAA GCAAACCTCA AGATGCCTCT TGTCTGCCAG CGTTGAAAGT TTCCGATATT
    GAACCCACCA TACCTGTCAC AGAGTTGGAC GTGGTCCTGA CAGCTGGAGA TATCCCTGTT
    CAGTACTGCG CCCAGCCCAC CAATGGCATG GTGTATTTCC GGGCCTTCTC CAGCCTGAAC
    ACACTCCCCG AGGAGCTGAG GCCCTATGTG CCCCTCTTCT GCAGCGTCCT CACCAACCCG
    TGCTTTGAAG AAGAGGAGCA CTTCAAGGTG CTGGTGAAGA TGACCGCCCA GGAGCTCGCC
    AATGGAATTC CTGACTCTGG GCACCTGTAC GCATCCATCA GGGCAGGCCG GACCCTCACG
    CCCGCAGGGG ACCTGCAGGA GACCTTCAGC GGGATGGATC AGGTGCGGCT GATGAAGAGG
    ATTGCAGAAA TGACAGATAT CAAACCCATC CTGAGGAAGC TCCCGCGTAT CAAGAAACAC
    TTGTTAAATG GTGATAATAT GAGGTGTTCA GTGAATGCGA CTCCTCAGCA GATGCCTCAG
    ACAGAAAAAG CGGTCGAAGA CTTCCTTAGA AGCATCGGTC GGAGTAAAAA GGAACGGAGG
    CCTGTGCGCC CACACACGGT CGAGAAACCT GTGCCCAGCA GCTCTGGTGG AGATGCCCAC
    GTTCCCCATG GCTCCCAGGT CATTAGGAAG CTGGTCATGG AACCCACCTT CAAGCCCTGG
    CAGATGAAGA CTCACTTCCT GATGCCCTTC CCGGTGAATT ACGTGGGTGA ATGCATCCGA
    ACTGTCCCCT ACACGGACCC AGATCATGCC AGTCTTAAAA TCCTTGCACG TTTGATGACT
    GCCAAATTCT TGCATACAGA AATTCGAGAA AAAGGCGGTG CTTATGGTGG AGGCGCAAAA
    CTCAGCCACA ATGGGATTTT CACCCTTTAC TCTTACAGGG ACCCAAATAC AATAGAGACG
    CTCCAGTCTT TTGGGAAGGC TGTCGACTGG GCTAAGTCTG GAAAATTCAC ACAGCAAGAC
    ATCGACGAAG CCAAACTTTC TGTCTTCTCA ACCGTAGATG CTCCTGTCGC TCCTTCAGAC
    AAAGGAATGG ACCACTTCTT GTACGGCCTC TCGGATGAGA TGAAGCAGGC CCACAGAGAG
    CAGCTCTTTG CTGTCAGCCA CGACAAGCTC CTGGCCGTGA GCGATAGATA CCTCGGCACT
    GGGAAGAGCA CACACGGCCT GGCCATCCTC GGACCCGAGA ACCCGAAAAT TGCCAAGGAC
    CCATCCTGGA TCATCCAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu43656
    Clone ID Related Accession (Same CDS sequence) NM_001347725.2 , NM_001347725.1
    Accession Version NM_001347725.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2916bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-25
    Organism Homo sapiens(human)
    Product presequence protease, mitochondrial isoform 4 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL451164.13. On May 31, 2019 this sequence version replaced NM_001347725.1. Transcript Variant: This variant (4) encodes isoform 4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.274837.1, SRR1803614.204696.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTGGCGCT GCGGCGGGCG GCAGGGCCTG TGTGTGCTGA GGCGGCTGAG CGGCGGACAT 
    GCACACCACA GAGCGTGGCG ATGGAACAGT AACCGGGCTT GTGAGAGGGC TCTGCAGTAT
    AAACTAGGAG ACAAGATCCA TGGATTCACC GTAAACCAGG TGACATCTGT TCCCGAGCTG
    TTCCTGACTG CAGTGAAGCT CACCCATGAT GACACAGGAG CCAGGTATTT ACACCTGGCC
    AGAGAAGACA CGAATAATCT GTTCAGCGTG CAGTTCCGTA CTACTCCCAT GGACAGTACT
    GGTGTTCCTC ACATTCTTGA GCATACCGTC CTTTGTGGGT CTCAGAAATA TCCGTGCAGA
    GACCCTTTCT TCAAAATGTT GAACCGGTCC CTCTCCACGT TCATGAACGC CTTCACAGCT
    AGTGATTATA CTCTGTATCC ATTTTCCACA CAAAATCCCA AGGACTTTCA GAATCTCCTC
    TCGGTGTATT TGGATGCCAC CTTTTTCCCA TGTTTACGCG AGCTGGATTT CTGGCAGGAA
    GGATGGCGGC TGGAACATGA GAATCCGAGC GACCCCCAGA CGCCCTTGGT CTTTAAAGGA
    GTCGTCTTTA ATGAGATGAA GGGAGCGTTT ACAGACAATG AGAGGATATT CTCCCAGCAC
    CTTCAGAACA GACTTCTTCC TGACCACACG TACTCAGTGG TCTCCGGGGG TGACCCACTG
    TGCATCCCGG AGCTTACATG GGAGCAGCTT AAGCAGTTTC ATGCCACTCA CTATCACCCA
    AGCAATGCTA GGTTCTTCAC GTACGGTAAT TTTCCATTAG AACAGCATCT GAAACAAATT
    CACGAGGAAG CACTGAGCAA ATTCCAGAAA ATTGAACCAA GCACCGTGGT GCCAGCTCAG
    ACACCCTGGG ACAAGCCTAG GGAATTCCAG ATAACATGTG GCCCGGATTC ATTTGCTACA
    GATCCCTCTA AACAAACAAC CATCAGCGTT AGCTTCCTCT TACCGGACAT CACCGACACA
    TTTGAAGCCT TCACATTAAG TCTTCTGTCT TCACTCTTGA CTTCTGGGCC CAATTCTCCC
    TTTTACAAAG CCTTGATTGA ATCTGGCCTT GGCACAGACT TTTCTCCTGA TGTTGGATAT
    AATGGCTACA CGAGGGAGGC CTACTTTAGT GTCGGCCTCC AAGGGATTGC GGAGAAAGAC
    ATTGAGACCG TCAGAAGCCT CATAGACAGA ACGATTGATG AAGTAGTTGA GAAAGGATTT
    GAAGATGATC GAATTGAGGC TTTACTTCAT AAAATTGAAA TACAGATGAA ACATCAGTCT
    ACCAGCTTTG GGCTGATGCT GACATCATAC ATAGCTTCTT GCTGGAACCA TGATGGGGAC
    CCTGTGGAGC TCTTGAAGTT GGGAAATCAG TTAGCTAAAT TCAGACAGTG CCTGCAGGAA
    AATCCAAAAT TTTTGCAAGA AAAAGTAAAA CAGTATTTTA AGAATAACCA GCATAAGCTG
    ACTTTATCGA TGAGGCCAGA TGACAAGTAT CACGAGAAGC AGGCACAGGT GGAAGCCACG
    AAGCTCAAGC AGAAGGTCGA GGCTCTGTCC CCCGGAGACA GGCAGCAGAT CTACGAGAAA
    GGTCTAGAAT TACGGAGTCA ACAAAGCAAA CCTCAAGATG CCTCTTGTCT GCCAGCGTTG
    AAAGTTTCCG ATATTGAACC CACCATACCT GTCACAGAGT TGGACGTGGT CCTGACAGCT
    GGAGATATCC CTGTTCAGTA CTGCGCCCAG CCCACCAATG GCATGGTGTA TTTCCGGGCC
    TTCTCCAGCC TGAACACACT CCCCGAGGAG CTGAGGCCCT ATGTGCCCCT CTTCTGCAGC
    GTCCTCACCA ACCCGTGCTT TGAAGAAGAG GAGCACTTCA AGGTGCTGGT GAAGATGACC
    GCCCAGGAGC TCGCCAATGG AATTCCTGAC TCTGGGCACC TGTACGCATC CATCAGGGCA
    GGCCGGACCC TCACGCCCGC AGGGGACCTG CAGGAGACCT TCAGCGGGAT GGATCAGGTG
    CGGCTGATGA AGAGGATTGC AGAAATGACA GATATCAAAC CCATCCTGAG GAAGCTCCCG
    CGTATCAAGA AACACTTGTT AAATGGTGAT AATATGAGGT GTTCAGTGAA TGCGACTCCT
    CAGCAGATGC CTCAGACAGA AAAAGCGGTC GAAGACTTCC TTAGAAGCAT CGGTCGGAGT
    AAAAAGGAAC GGAGGCCTGT GCGCCCACAC ACGGTCGAGA AACCTGTGCC CAGCAGCTCT
    GGTGGAGATG CCCACGTTCC CCATGGCTCC CAGGTCATTA GGAAGCTGGT CATGGAACCC
    ACCTTCAAGC CCTGGCAGAT GAAGACTCAC TTCCTGATGC CCTTCCCGGT GAATTACGTG
    GGTGAATGCA TCCGAACTGT CCCCTACACG GACCCAGATC ATGCCAGTCT TAAAATCCTT
    GCACGTTTGA TGACTGCCAA ATTCTTGCAT ACAGAAATTC GAGAAAAAGG CGGTGCTTAT
    GGTGGAGGCG CAAAACTCAG CCACAATGGG ATTTTCACCC TTTACTCTTA CAGGGACCCA
    AATACAATAG AGACGCTCCA GTCTTTTGGG AAGGCTGTCG ACTGGGCTAA GTCTGGAAAA
    TTCACACAGC AAGACATCGA CGAAGCCAAA CTTTCTGTCT TCTCAACCGT AGATGCTCCT
    GTCGCTCCTT CAGACAAAGG AATGGACCAC TTCTTGTACG GCCTCTCGGA TGAGATGAAG
    CAGGCCCACA GAGAGCAGCT CTTTGCTGTC AGCCACGACA AGCTCCTGGC CGTGAGCGAT
    AGATACCTCG GCACTGGGAA GAGCACACAC GGCCTGGCCA TCCTCGGACC CGAGAACCCG
    AAAATTGCCA AGGACCCATC CTGGATCATC CAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334654.1
    CDS11..2926
    Translation

    Target ORF information:

    RefSeq Version NM_001347725.2
    Organism Homo sapiens(human)
    Definition Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347725.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTGGCGCT GCGGCGGGCG GCAGGGCCTG TGTGTGCTGA GGCGGCTGAG CGGCGGACAT 
    GCACACCACA GAGCGTGGCG ATGGAACAGT AACCGGGCTT GTGAGAGGGC TCTGCAGTAT
    AAACTAGGAG ACAAGATCCA TGGATTCACC GTAAACCAGG TGACATCTGT TCCCGAGCTG
    TTCCTGACTG CAGTGAAGCT CACCCATGAT GACACAGGAG CCAGGTATTT ACACCTGGCC
    AGAGAAGACA CGAATAATCT GTTCAGCGTG CAGTTCCGTA CTACTCCCAT GGACAGTACT
    GGTGTTCCTC ACATTCTTGA GCATACCGTC CTTTGTGGGT CTCAGAAATA TCCGTGCAGA
    GACCCTTTCT TCAAAATGTT GAACCGGTCC CTCTCCACGT TCATGAACGC CTTCACAGCT
    AGTGATTATA CTCTGTATCC ATTTTCCACA CAAAATCCCA AGGACTTTCA GAATCTCCTC
    TCGGTGTATT TGGATGCCAC CTTTTTCCCA TGTTTACGCG AGCTGGATTT CTGGCAGGAA
    GGATGGCGGC TGGAACATGA GAATCCGAGC GACCCCCAGA CGCCCTTGGT CTTTAAAGGA
    GTCGTCTTTA ATGAGATGAA GGGAGCGTTT ACAGACAATG AGAGGATATT CTCCCAGCAC
    CTTCAGAACA GACTTCTTCC TGACCACACG TACTCAGTGG TCTCCGGGGG TGACCCACTG
    TGCATCCCGG AGCTTACATG GGAGCAGCTT AAGCAGTTTC ATGCCACTCA CTATCACCCA
    AGCAATGCTA GGTTCTTCAC GTACGGTAAT TTTCCATTAG AACAGCATCT GAAACAAATT
    CACGAGGAAG CACTGAGCAA ATTCCAGAAA ATTGAACCAA GCACCGTGGT GCCAGCTCAG
    ACACCCTGGG ACAAGCCTAG GGAATTCCAG ATAACATGTG GCCCGGATTC ATTTGCTACA
    GATCCCTCTA AACAAACAAC CATCAGCGTT AGCTTCCTCT TACCGGACAT CACCGACACA
    TTTGAAGCCT TCACATTAAG TCTTCTGTCT TCACTCTTGA CTTCTGGGCC CAATTCTCCC
    TTTTACAAAG CCTTGATTGA ATCTGGCCTT GGCACAGACT TTTCTCCTGA TGTTGGATAT
    AATGGCTACA CGAGGGAGGC CTACTTTAGT GTCGGCCTCC AAGGGATTGC GGAGAAAGAC
    ATTGAGACCG TCAGAAGCCT CATAGACAGA ACGATTGATG AAGTAGTTGA GAAAGGATTT
    GAAGATGATC GAATTGAGGC TTTACTTCAT AAAATTGAAA TACAGATGAA ACATCAGTCT
    ACCAGCTTTG GGCTGATGCT GACATCATAC ATAGCTTCTT GCTGGAACCA TGATGGGGAC
    CCTGTGGAGC TCTTGAAGTT GGGAAATCAG TTAGCTAAAT TCAGACAGTG CCTGCAGGAA
    AATCCAAAAT TTTTGCAAGA AAAAGTAAAA CAGTATTTTA AGAATAACCA GCATAAGCTG
    ACTTTATCGA TGAGGCCAGA TGACAAGTAT CACGAGAAGC AGGCACAGGT GGAAGCCACG
    AAGCTCAAGC AGAAGGTCGA GGCTCTGTCC CCCGGAGACA GGCAGCAGAT CTACGAGAAA
    GGTCTAGAAT TACGGAGTCA ACAAAGCAAA CCTCAAGATG CCTCTTGTCT GCCAGCGTTG
    AAAGTTTCCG ATATTGAACC CACCATACCT GTCACAGAGT TGGACGTGGT CCTGACAGCT
    GGAGATATCC CTGTTCAGTA CTGCGCCCAG CCCACCAATG GCATGGTGTA TTTCCGGGCC
    TTCTCCAGCC TGAACACACT CCCCGAGGAG CTGAGGCCCT ATGTGCCCCT CTTCTGCAGC
    GTCCTCACCA ACCCGTGCTT TGAAGAAGAG GAGCACTTCA AGGTGCTGGT GAAGATGACC
    GCCCAGGAGC TCGCCAATGG AATTCCTGAC TCTGGGCACC TGTACGCATC CATCAGGGCA
    GGCCGGACCC TCACGCCCGC AGGGGACCTG CAGGAGACCT TCAGCGGGAT GGATCAGGTG
    CGGCTGATGA AGAGGATTGC AGAAATGACA GATATCAAAC CCATCCTGAG GAAGCTCCCG
    CGTATCAAGA AACACTTGTT AAATGGTGAT AATATGAGGT GTTCAGTGAA TGCGACTCCT
    CAGCAGATGC CTCAGACAGA AAAAGCGGTC GAAGACTTCC TTAGAAGCAT CGGTCGGAGT
    AAAAAGGAAC GGAGGCCTGT GCGCCCACAC ACGGTCGAGA AACCTGTGCC CAGCAGCTCT
    GGTGGAGATG CCCACGTTCC CCATGGCTCC CAGGTCATTA GGAAGCTGGT CATGGAACCC
    ACCTTCAAGC CCTGGCAGAT GAAGACTCAC TTCCTGATGC CCTTCCCGGT GAATTACGTG
    GGTGAATGCA TCCGAACTGT CCCCTACACG GACCCAGATC ATGCCAGTCT TAAAATCCTT
    GCACGTTTGA TGACTGCCAA ATTCTTGCAT ACAGAAATTC GAGAAAAAGG CGGTGCTTAT
    GGTGGAGGCG CAAAACTCAG CCACAATGGG ATTTTCACCC TTTACTCTTA CAGGGACCCA
    AATACAATAG AGACGCTCCA GTCTTTTGGG AAGGCTGTCG ACTGGGCTAA GTCTGGAAAA
    TTCACACAGC AAGACATCGA CGAAGCCAAA CTTTCTGTCT TCTCAACCGT AGATGCTCCT
    GTCGCTCCTT CAGACAAAGG AATGGACCAC TTCTTGTACG GCCTCTCGGA TGAGATGAAG
    CAGGCCCACA GAGAGCAGCT CTTTGCTGTC AGCCACGACA AGCTCCTGGC CGTGAGCGAT
    AGATACCTCG GCACTGGGAA GAGCACACAC GGCCTGGCCA TCCTCGGACC CGAGAACCCG
    AAAATTGCCA AGGACCCATC CTGGATCATC CAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu43656
    Clone ID Related Accession (Same CDS sequence) NM_001347725.2 , NM_001347725.1
    Accession Version NM_001347725.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2916bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product presequence protease, mitochondrial isoform 4 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL451164.13. On Dec 13, 2016 this sequence version replaced XM_005252345.3. Transcript Variant: This variant (4) encodes isoform 4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.123512.1, SRR1803611.84793.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTGGCGCT GCGGCGGGCG GCAGGGCCTG TGTGTGCTGA GGCGGCTGAG CGGCGGACAT 
    GCACACCACA GAGCGTGGCG ATGGAACAGT AACCGGGCTT GTGAGAGGGC TCTGCAGTAT
    AAACTAGGAG ACAAGATCCA TGGATTCACC GTAAACCAGG TGACATCTGT TCCCGAGCTG
    TTCCTGACTG CAGTGAAGCT CACCCATGAT GACACAGGAG CCAGGTATTT ACACCTGGCC
    AGAGAAGACA CGAATAATCT GTTCAGCGTG CAGTTCCGTA CTACTCCCAT GGACAGTACT
    GGTGTTCCTC ACATTCTTGA GCATACCGTC CTTTGTGGGT CTCAGAAATA TCCGTGCAGA
    GACCCTTTCT TCAAAATGTT GAACCGGTCC CTCTCCACGT TCATGAACGC CTTCACAGCT
    AGTGATTATA CTCTGTATCC ATTTTCCACA CAAAATCCCA AGGACTTTCA GAATCTCCTC
    TCGGTGTATT TGGATGCCAC CTTTTTCCCA TGTTTACGCG AGCTGGATTT CTGGCAGGAA
    GGATGGCGGC TGGAACATGA GAATCCGAGC GACCCCCAGA CGCCCTTGGT CTTTAAAGGA
    GTCGTCTTTA ATGAGATGAA GGGAGCGTTT ACAGACAATG AGAGGATATT CTCCCAGCAC
    CTTCAGAACA GACTTCTTCC TGACCACACG TACTCAGTGG TCTCCGGGGG TGACCCACTG
    TGCATCCCGG AGCTTACATG GGAGCAGCTT AAGCAGTTTC ATGCCACTCA CTATCACCCA
    AGCAATGCTA GGTTCTTCAC GTACGGTAAT TTTCCATTAG AACAGCATCT GAAACAAATT
    CACGAGGAAG CACTGAGCAA ATTCCAGAAA ATTGAACCAA GCACCGTGGT GCCAGCTCAG
    ACACCCTGGG ACAAGCCTAG GGAATTCCAG ATAACATGTG GCCCGGATTC ATTTGCTACA
    GATCCCTCTA AACAAACAAC CATCAGCGTT AGCTTCCTCT TACCGGACAT CACCGACACA
    TTTGAAGCCT TCACATTAAG TCTTCTGTCT TCACTCTTGA CTTCTGGGCC CAATTCTCCC
    TTTTACAAAG CCTTGATTGA ATCTGGCCTT GGCACAGACT TTTCTCCTGA TGTTGGATAT
    AATGGCTACA CGAGGGAGGC CTACTTTAGT GTCGGCCTCC AAGGGATTGC GGAGAAAGAC
    ATTGAGACCG TCAGAAGCCT CATAGACAGA ACGATTGATG AAGTAGTTGA GAAAGGATTT
    GAAGATGATC GAATTGAGGC TTTACTTCAT AAAATTGAAA TACAGATGAA ACATCAGTCT
    ACCAGCTTTG GGCTGATGCT GACATCATAC ATAGCTTCTT GCTGGAACCA TGATGGGGAC
    CCTGTGGAGC TCTTGAAGTT GGGAAATCAG TTAGCTAAAT TCAGACAGTG CCTGCAGGAA
    AATCCAAAAT TTTTGCAAGA AAAAGTAAAA CAGTATTTTA AGAATAACCA GCATAAGCTG
    ACTTTATCGA TGAGGCCAGA TGACAAGTAT CACGAGAAGC AGGCACAGGT GGAAGCCACG
    AAGCTCAAGC AGAAGGTCGA GGCTCTGTCC CCCGGAGACA GGCAGCAGAT CTACGAGAAA
    GGTCTAGAAT TACGGAGTCA ACAAAGCAAA CCTCAAGATG CCTCTTGTCT GCCAGCGTTG
    AAAGTTTCCG ATATTGAACC CACCATACCT GTCACAGAGT TGGACGTGGT CCTGACAGCT
    GGAGATATCC CTGTTCAGTA CTGCGCCCAG CCCACCAATG GCATGGTGTA TTTCCGGGCC
    TTCTCCAGCC TGAACACACT CCCCGAGGAG CTGAGGCCCT ATGTGCCCCT CTTCTGCAGC
    GTCCTCACCA ACCCGTGCTT TGAAGAAGAG GAGCACTTCA AGGTGCTGGT GAAGATGACC
    GCCCAGGAGC TCGCCAATGG AATTCCTGAC TCTGGGCACC TGTACGCATC CATCAGGGCA
    GGCCGGACCC TCACGCCCGC AGGGGACCTG CAGGAGACCT TCAGCGGGAT GGATCAGGTG
    CGGCTGATGA AGAGGATTGC AGAAATGACA GATATCAAAC CCATCCTGAG GAAGCTCCCG
    CGTATCAAGA AACACTTGTT AAATGGTGAT AATATGAGGT GTTCAGTGAA TGCGACTCCT
    CAGCAGATGC CTCAGACAGA AAAAGCGGTC GAAGACTTCC TTAGAAGCAT CGGTCGGAGT
    AAAAAGGAAC GGAGGCCTGT GCGCCCACAC ACGGTCGAGA AACCTGTGCC CAGCAGCTCT
    GGTGGAGATG CCCACGTTCC CCATGGCTCC CAGGTCATTA GGAAGCTGGT CATGGAACCC
    ACCTTCAAGC CCTGGCAGAT GAAGACTCAC TTCCTGATGC CCTTCCCGGT GAATTACGTG
    GGTGAATGCA TCCGAACTGT CCCCTACACG GACCCAGATC ATGCCAGTCT TAAAATCCTT
    GCACGTTTGA TGACTGCCAA ATTCTTGCAT ACAGAAATTC GAGAAAAAGG CGGTGCTTAT
    GGTGGAGGCG CAAAACTCAG CCACAATGGG ATTTTCACCC TTTACTCTTA CAGGGACCCA
    AATACAATAG AGACGCTCCA GTCTTTTGGG AAGGCTGTCG ACTGGGCTAA GTCTGGAAAA
    TTCACACAGC AAGACATCGA CGAAGCCAAA CTTTCTGTCT TCTCAACCGT AGATGCTCCT
    GTCGCTCCTT CAGACAAAGG AATGGACCAC TTCTTGTACG GCCTCTCGGA TGAGATGAAG
    CAGGCCCACA GAGAGCAGCT CTTTGCTGTC AGCCACGACA AGCTCCTGGC CGTGAGCGAT
    AGATACCTCG GCACTGGGAA GAGCACACAC GGCCTGGCCA TCCTCGGACC CGAGAACCCG
    AAAATTGCCA AGGACCCATC CTGGATCATC CAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334654.1
    CDS70..2985
    Translation

    Target ORF information:

    RefSeq Version NM_001347725.1
    Organism Homo sapiens(human)
    Definition Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347725.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTGGCGCT GCGGCGGGCG GCAGGGCCTG TGTGTGCTGA GGCGGCTGAG CGGCGGACAT 
    GCACACCACA GAGCGTGGCG ATGGAACAGT AACCGGGCTT GTGAGAGGGC TCTGCAGTAT
    AAACTAGGAG ACAAGATCCA TGGATTCACC GTAAACCAGG TGACATCTGT TCCCGAGCTG
    TTCCTGACTG CAGTGAAGCT CACCCATGAT GACACAGGAG CCAGGTATTT ACACCTGGCC
    AGAGAAGACA CGAATAATCT GTTCAGCGTG CAGTTCCGTA CTACTCCCAT GGACAGTACT
    GGTGTTCCTC ACATTCTTGA GCATACCGTC CTTTGTGGGT CTCAGAAATA TCCGTGCAGA
    GACCCTTTCT TCAAAATGTT GAACCGGTCC CTCTCCACGT TCATGAACGC CTTCACAGCT
    AGTGATTATA CTCTGTATCC ATTTTCCACA CAAAATCCCA AGGACTTTCA GAATCTCCTC
    TCGGTGTATT TGGATGCCAC CTTTTTCCCA TGTTTACGCG AGCTGGATTT CTGGCAGGAA
    GGATGGCGGC TGGAACATGA GAATCCGAGC GACCCCCAGA CGCCCTTGGT CTTTAAAGGA
    GTCGTCTTTA ATGAGATGAA GGGAGCGTTT ACAGACAATG AGAGGATATT CTCCCAGCAC
    CTTCAGAACA GACTTCTTCC TGACCACACG TACTCAGTGG TCTCCGGGGG TGACCCACTG
    TGCATCCCGG AGCTTACATG GGAGCAGCTT AAGCAGTTTC ATGCCACTCA CTATCACCCA
    AGCAATGCTA GGTTCTTCAC GTACGGTAAT TTTCCATTAG AACAGCATCT GAAACAAATT
    CACGAGGAAG CACTGAGCAA ATTCCAGAAA ATTGAACCAA GCACCGTGGT GCCAGCTCAG
    ACACCCTGGG ACAAGCCTAG GGAATTCCAG ATAACATGTG GCCCGGATTC ATTTGCTACA
    GATCCCTCTA AACAAACAAC CATCAGCGTT AGCTTCCTCT TACCGGACAT CACCGACACA
    TTTGAAGCCT TCACATTAAG TCTTCTGTCT TCACTCTTGA CTTCTGGGCC CAATTCTCCC
    TTTTACAAAG CCTTGATTGA ATCTGGCCTT GGCACAGACT TTTCTCCTGA TGTTGGATAT
    AATGGCTACA CGAGGGAGGC CTACTTTAGT GTCGGCCTCC AAGGGATTGC GGAGAAAGAC
    ATTGAGACCG TCAGAAGCCT CATAGACAGA ACGATTGATG AAGTAGTTGA GAAAGGATTT
    GAAGATGATC GAATTGAGGC TTTACTTCAT AAAATTGAAA TACAGATGAA ACATCAGTCT
    ACCAGCTTTG GGCTGATGCT GACATCATAC ATAGCTTCTT GCTGGAACCA TGATGGGGAC
    CCTGTGGAGC TCTTGAAGTT GGGAAATCAG TTAGCTAAAT TCAGACAGTG CCTGCAGGAA
    AATCCAAAAT TTTTGCAAGA AAAAGTAAAA CAGTATTTTA AGAATAACCA GCATAAGCTG
    ACTTTATCGA TGAGGCCAGA TGACAAGTAT CACGAGAAGC AGGCACAGGT GGAAGCCACG
    AAGCTCAAGC AGAAGGTCGA GGCTCTGTCC CCCGGAGACA GGCAGCAGAT CTACGAGAAA
    GGTCTAGAAT TACGGAGTCA ACAAAGCAAA CCTCAAGATG CCTCTTGTCT GCCAGCGTTG
    AAAGTTTCCG ATATTGAACC CACCATACCT GTCACAGAGT TGGACGTGGT CCTGACAGCT
    GGAGATATCC CTGTTCAGTA CTGCGCCCAG CCCACCAATG GCATGGTGTA TTTCCGGGCC
    TTCTCCAGCC TGAACACACT CCCCGAGGAG CTGAGGCCCT ATGTGCCCCT CTTCTGCAGC
    GTCCTCACCA ACCCGTGCTT TGAAGAAGAG GAGCACTTCA AGGTGCTGGT GAAGATGACC
    GCCCAGGAGC TCGCCAATGG AATTCCTGAC TCTGGGCACC TGTACGCATC CATCAGGGCA
    GGCCGGACCC TCACGCCCGC AGGGGACCTG CAGGAGACCT TCAGCGGGAT GGATCAGGTG
    CGGCTGATGA AGAGGATTGC AGAAATGACA GATATCAAAC CCATCCTGAG GAAGCTCCCG
    CGTATCAAGA AACACTTGTT AAATGGTGAT AATATGAGGT GTTCAGTGAA TGCGACTCCT
    CAGCAGATGC CTCAGACAGA AAAAGCGGTC GAAGACTTCC TTAGAAGCAT CGGTCGGAGT
    AAAAAGGAAC GGAGGCCTGT GCGCCCACAC ACGGTCGAGA AACCTGTGCC CAGCAGCTCT
    GGTGGAGATG CCCACGTTCC CCATGGCTCC CAGGTCATTA GGAAGCTGGT CATGGAACCC
    ACCTTCAAGC CCTGGCAGAT GAAGACTCAC TTCCTGATGC CCTTCCCGGT GAATTACGTG
    GGTGAATGCA TCCGAACTGT CCCCTACACG GACCCAGATC ATGCCAGTCT TAAAATCCTT
    GCACGTTTGA TGACTGCCAA ATTCTTGCAT ACAGAAATTC GAGAAAAAGG CGGTGCTTAT
    GGTGGAGGCG CAAAACTCAG CCACAATGGG ATTTTCACCC TTTACTCTTA CAGGGACCCA
    AATACAATAG AGACGCTCCA GTCTTTTGGG AAGGCTGTCG ACTGGGCTAA GTCTGGAAAA
    TTCACACAGC AAGACATCGA CGAAGCCAAA CTTTCTGTCT TCTCAACCGT AGATGCTCCT
    GTCGCTCCTT CAGACAAAGG AATGGACCAC TTCTTGTACG GCCTCTCGGA TGAGATGAAG
    CAGGCCCACA GAGAGCAGCT CTTTGCTGTC AGCCACGACA AGCTCCTGGC CGTGAGCGAT
    AGATACCTCG GCACTGGGAA GAGCACACAC GGCCTGGCCA TCCTCGGACC CGAGAACCCG
    AAAATTGCCA AGGACCCATC CTGGATCATC CAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101478
    Clone ID Related Accession (Same CDS sequence) NM_001347730.1
    Accession Version NM_001347730.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2892bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-22
    Organism Homo sapiens(human)
    Product presequence protease, mitochondrial isoform 9
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL451164.13. On Dec 13, 2016 this sequence version replaced XM_017015471.1. Transcript Variant: This variant (9) encodes isoform 9, which likely does not localize to the mitochondrion. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC045577.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGTAATCC ACGAGCCCGT GTTTAATATA AGACATGCAC ACCACAGAGC GTGGCGATGG 
    AACAGTAACC GGGCTTGTGA GAGGGCTCTG CAGTATAAAC TAGGAGACAA GATCCATGGA
    TTCACCGTAA ACCAGGTGAC ATCTGTTCCC GAGCTGTTCC TGACTGCAGT GAAGCTCACC
    CATGATGACA CAGGAGCCAG GTATTTACAC CTGGCCAGAG AAGACACGAA TAATCTGTTC
    AGCGTGCAGT TCCGTACTAC TCCCATGGAC AGTACTGGTG TTCCTCACAT TCTTGAGCAT
    ACCGTCCTTT GTGGGTCTCA GAAATATCCG TGCAGAGACC CTTTCTTCAA AATGTTGAAC
    CGGTCCCTCT CCACGTTCAT GAACGCCTTC ACAGCTAGTG ATTATACTCT GTATCCATTT
    TCCACACAAA ATCCCAAGGA CTTTCAGAAT CTCCTCTCGG TGTATTTGGA TGCCACCTTT
    TTCCCATGTT TACGCGAGCT GGATTTCTGG CAGGAAGGAT GGCGGCTGGA ACATGAGAAT
    CCGAGCGACC CCCAGACGCC CTTGGTCTTT AAAGGAGTCG TCTTTAATGA GATGAAGGGA
    GCGTTTACAG ACAATGAGAG GATATTCTCC CAGCACCTTC AGAACAGACT TCTTCCTGAC
    CACACGTACT CAGTGGTCTC CGGGGGTGAC CCACTGTGCA TCCCGGAGCT TACATGGGAG
    CAGCTTAAGC AGTTTCATGC CACTCACTAT CACCCAAGCA ATGCTAGGTT CTTCACGTAC
    GGTAATTTTC CATTAGAACA GCATCTGAAA CAAATTCACG AGGAAGCACT GAGCAAATTC
    CAGAAAATTG AACCAAGCAC CGTGGTGCCA GCTCAGACAC CCTGGGACAA GCCTAGGGAA
    TTCCAGATAA CATGTGGCCC GGATTCATTT GCTACAGATC CCTCTAAACA AACAACCATC
    AGCGTTAGCT TCCTCTTACC GGACATCACC GACACATTTG AAGCCTTCAC ATTAAGTCTT
    CTGTCTTCAC TCTTGACTTC TGGGCCCAAT TCTCCCTTTT ACAAAGCCTT GATTGAATCT
    GGCCTTGGCA CAGACTTTTC TCCTGATGTT GGATATAATG GCTACACGAG GGAGGCCTAC
    TTTAGTGTCG GCCTCCAAGG GATTGCGGAG AAAGACATTG AGACCGTCAG AAGCCTCATA
    GACAGAACGA TTGATGAAGT AGTTGAGAAA GGATTTGAAG ATGATCGAAT TGAGGCTTTA
    CTTCATAAAA TTGAAATACA GATGAAACAT CAGTCTACCA GCTTTGGGCT GATGCTGACA
    TCATACATAG CTTCTTGCTG GAACCATGAT GGGGACCCTG TGGAGCTCTT GAAGTTGGGA
    AATCAGTTAG CTAAATTCAG ACAGTGCCTG CAGGAAAATC CAAAATTTTT GCAAGAAAAA
    GTAAAACAGT ATTTTAAGAA TAACCAGCAT AAGCTGACTT TATCGATGAG GCCAGATGAC
    AAGTATCACG AGAAGCAGGC ACAGGTGGAA GCCACGAAGC TCAAGCAGAA GGTCGAGGCT
    CTGTCCCCCG GAGACAGGCA GCAGATCTAC GAGAAAGGTC TAGAATTACG GAGTCAACAA
    AGCAAACCTC AAGATGCCTC TTGTCTGCCA GCGTTGAAAG TTTCCGATAT TGAACCCACC
    ATACCTGTCA CAGAGTTGGA CGTGGTCCTG ACAGCTGGAG ATATCCCTGT TCAGTACTGC
    GCCCAGCCCA CCAATGGCAT GGTGTATTTC CGGGCCTTCT CCAGCCTGAA CACACTCCCC
    GAGGAGCTGA GGCCCTATGT GCCCCTCTTC TGCAGCGTCC TCACCAACCC GTGCTTTGAA
    GAAGAGGAGC ACTTCAAGGT GCTGGTGAAG ATGACCGCCC AGGAGCTCGC CAATGGAATT
    CCTGACTCTG GGCACCTGTA CGCATCCATC AGGGCAGGCC GGACCCTCAC GCCCGCAGGG
    GACCTGCAGG AGACCTTCAG CGGGATGGAT CAGGTGCGGC TGATGAAGAG GATTGCAGAA
    ATGACAGATA TCAAACCCAT CCTGAGGAAG CTCCCGCGTA TCAAGAAACA CTTGTTAAAT
    GGTGATAATA TGAGGTGTTC AGTGAATGCG ACTCCTCAGC AGATGCCTCA GACAGAAAAA
    GCGGTCGAAG ACTTCCTTAG AAGCATCGGT CGGAGTAAAA AGGAACGGAG GCCTGTGCGC
    CCACACACGG TCGAGAAACC TGTGCCCAGC AGCTCTGGTG GAGATGCCCA CGTTCCCCAT
    GGCTCCCAGG TCATTAGGAA GCTGGTCATG GAACCCACCT TCAAGCCCTG GCAGATGAAG
    ACTCACTTCC TGATGCCCTT CCCGGTGAAT TACGTGGGTG AATGCATCCG AACTGTCCCC
    TACACGGACC CAGATCATGC CAGTCTTAAA ATCCTTGCAC GTTTGATGAC TGCCAAATTC
    TTGCATACAG AAATTCGAGA AAAAGGCGGT GCTTATGGTG GAGGCGCAAA ACTCAGCCAC
    AATGGGATTT TCACCCTTTA CTCTTACAGG GACCCAAATA CAATAGAGAC GCTCCAGTCT
    TTTGGGAAGG CTGTCGACTG GGCTAAGTCT GGAAAATTCA CACAGCAAGA CATCGACGAA
    GCCAAACTTT CTGTCTTCTC AACCGTAGAT GCTCCTGTCG CTCCTTCAGA CAAAGGAATG
    GACCACTTCT TGTACGGCCT CTCGGATGAG ATGAAGCAGG CCCACAGAGA GCAGCTCTTT
    GCTGTCAGCC ACGACAAGCT CCTGGCCGTG AGCGATAGAT ACCTCGGCAC TGGGAAGAGC
    ACACACGGCC TGGCCATCCT CGGACCCGAG AACCCGAAAA TTGCCAAGGA CCCATCCTGG
    ATCATCCAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334659.1
    CDS504..3395
    Translation

    Target ORF information:

    RefSeq Version NM_001347730.1
    Organism Homo sapiens(human)
    Definition Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347730.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGTAATCC ACGAGCCCGT GTTTAATATA AGACATGCAC ACCACAGAGC GTGGCGATGG 
    AACAGTAACC GGGCTTGTGA GAGGGCTCTG CAGTATAAAC TAGGAGACAA GATCCATGGA
    TTCACCGTAA ACCAGGTGAC ATCTGTTCCC GAGCTGTTCC TGACTGCAGT GAAGCTCACC
    CATGATGACA CAGGAGCCAG GTATTTACAC CTGGCCAGAG AAGACACGAA TAATCTGTTC
    AGCGTGCAGT TCCGTACTAC TCCCATGGAC AGTACTGGTG TTCCTCACAT TCTTGAGCAT
    ACCGTCCTTT GTGGGTCTCA GAAATATCCG TGCAGAGACC CTTTCTTCAA AATGTTGAAC
    CGGTCCCTCT CCACGTTCAT GAACGCCTTC ACAGCTAGTG ATTATACTCT GTATCCATTT
    TCCACACAAA ATCCCAAGGA CTTTCAGAAT CTCCTCTCGG TGTATTTGGA TGCCACCTTT
    TTCCCATGTT TACGCGAGCT GGATTTCTGG CAGGAAGGAT GGCGGCTGGA ACATGAGAAT
    CCGAGCGACC CCCAGACGCC CTTGGTCTTT AAAGGAGTCG TCTTTAATGA GATGAAGGGA
    GCGTTTACAG ACAATGAGAG GATATTCTCC CAGCACCTTC AGAACAGACT TCTTCCTGAC
    CACACGTACT CAGTGGTCTC CGGGGGTGAC CCACTGTGCA TCCCGGAGCT TACATGGGAG
    CAGCTTAAGC AGTTTCATGC CACTCACTAT CACCCAAGCA ATGCTAGGTT CTTCACGTAC
    GGTAATTTTC CATTAGAACA GCATCTGAAA CAAATTCACG AGGAAGCACT GAGCAAATTC
    CAGAAAATTG AACCAAGCAC CGTGGTGCCA GCTCAGACAC CCTGGGACAA GCCTAGGGAA
    TTCCAGATAA CATGTGGCCC GGATTCATTT GCTACAGATC CCTCTAAACA AACAACCATC
    AGCGTTAGCT TCCTCTTACC GGACATCACC GACACATTTG AAGCCTTCAC ATTAAGTCTT
    CTGTCTTCAC TCTTGACTTC TGGGCCCAAT TCTCCCTTTT ACAAAGCCTT GATTGAATCT
    GGCCTTGGCA CAGACTTTTC TCCTGATGTT GGATATAATG GCTACACGAG GGAGGCCTAC
    TTTAGTGTCG GCCTCCAAGG GATTGCGGAG AAAGACATTG AGACCGTCAG AAGCCTCATA
    GACAGAACGA TTGATGAAGT AGTTGAGAAA GGATTTGAAG ATGATCGAAT TGAGGCTTTA
    CTTCATAAAA TTGAAATACA GATGAAACAT CAGTCTACCA GCTTTGGGCT GATGCTGACA
    TCATACATAG CTTCTTGCTG GAACCATGAT GGGGACCCTG TGGAGCTCTT GAAGTTGGGA
    AATCAGTTAG CTAAATTCAG ACAGTGCCTG CAGGAAAATC CAAAATTTTT GCAAGAAAAA
    GTAAAACAGT ATTTTAAGAA TAACCAGCAT AAGCTGACTT TATCGATGAG GCCAGATGAC
    AAGTATCACG AGAAGCAGGC ACAGGTGGAA GCCACGAAGC TCAAGCAGAA GGTCGAGGCT
    CTGTCCCCCG GAGACAGGCA GCAGATCTAC GAGAAAGGTC TAGAATTACG GAGTCAACAA
    AGCAAACCTC AAGATGCCTC TTGTCTGCCA GCGTTGAAAG TTTCCGATAT TGAACCCACC
    ATACCTGTCA CAGAGTTGGA CGTGGTCCTG ACAGCTGGAG ATATCCCTGT TCAGTACTGC
    GCCCAGCCCA CCAATGGCAT GGTGTATTTC CGGGCCTTCT CCAGCCTGAA CACACTCCCC
    GAGGAGCTGA GGCCCTATGT GCCCCTCTTC TGCAGCGTCC TCACCAACCC GTGCTTTGAA
    GAAGAGGAGC ACTTCAAGGT GCTGGTGAAG ATGACCGCCC AGGAGCTCGC CAATGGAATT
    CCTGACTCTG GGCACCTGTA CGCATCCATC AGGGCAGGCC GGACCCTCAC GCCCGCAGGG
    GACCTGCAGG AGACCTTCAG CGGGATGGAT CAGGTGCGGC TGATGAAGAG GATTGCAGAA
    ATGACAGATA TCAAACCCAT CCTGAGGAAG CTCCCGCGTA TCAAGAAACA CTTGTTAAAT
    GGTGATAATA TGAGGTGTTC AGTGAATGCG ACTCCTCAGC AGATGCCTCA GACAGAAAAA
    GCGGTCGAAG ACTTCCTTAG AAGCATCGGT CGGAGTAAAA AGGAACGGAG GCCTGTGCGC
    CCACACACGG TCGAGAAACC TGTGCCCAGC AGCTCTGGTG GAGATGCCCA CGTTCCCCAT
    GGCTCCCAGG TCATTAGGAA GCTGGTCATG GAACCCACCT TCAAGCCCTG GCAGATGAAG
    ACTCACTTCC TGATGCCCTT CCCGGTGAAT TACGTGGGTG AATGCATCCG AACTGTCCCC
    TACACGGACC CAGATCATGC CAGTCTTAAA ATCCTTGCAC GTTTGATGAC TGCCAAATTC
    TTGCATACAG AAATTCGAGA AAAAGGCGGT GCTTATGGTG GAGGCGCAAA ACTCAGCCAC
    AATGGGATTT TCACCCTTTA CTCTTACAGG GACCCAAATA CAATAGAGAC GCTCCAGTCT
    TTTGGGAAGG CTGTCGACTG GGCTAAGTCT GGAAAATTCA CACAGCAAGA CATCGACGAA
    GCCAAACTTT CTGTCTTCTC AACCGTAGAT GCTCCTGTCG CTCCTTCAGA CAAAGGAATG
    GACCACTTCT TGTACGGCCT CTCGGATGAG ATGAAGCAGG CCCACAGAGA GCAGCTCTTT
    GCTGTCAGCC ACGACAAGCT CCTGGCCGTG AGCGATAGAT ACCTCGGCAC TGGGAAGAGC
    ACACACGGCC TGGCCATCCT CGGACCCGAG AACCCGAAAA TTGCCAAGGA CCCATCCTGG
    ATCATCCAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101521
    Clone ID Related Accession (Same CDS sequence) NM_001347727.2 , NM_001347727.1
    Accession Version NM_001347727.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2499bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-25
    Organism Homo sapiens(human)
    Product presequence protease, mitochondrial isoform 6
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL451164.13. On May 31, 2019 this sequence version replaced NM_001347727.1. Transcript Variant: This variant (6) encodes isoform 6, which likely does not localize to the mitochondrion. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.56546.1, SRR1803613.41592.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAGGGAG CGTTTACAGA CAATGAGAGG ATATTCTCCC AGCACCTTCA GAACAGACTT 
    CTTCCTGACC ACACGTACTC AGTGGTCTCC GGGGGTGACC CACTGTGCAT CCCGGAGCTT
    ACATGGGAGC AGCTTAAGCA GTTTCATGCC ACTCACTATC ACCCAAGCAA TGCTAGGTTC
    TTCACGTACG GTAATTTTCC ATTAGAACAG CATCTGAAAC AAATTCACGA GGAAGCACTG
    AGCAAATTCC AGAAAATTGA ACCAAGCACC GTGGTGCCAG CTCAGACACC CTGGGACAAG
    CCTAGGGAAT TCCAGATAAC ATGTGGCCCG GATTCATTTG CTACAGATCC CTCTAAACAA
    ACAACCATCA GCGTTAGCTT CCTCTTACCG GACATCACCG ACACATTTGA AGCCTTCACA
    TTAAGTCTTC TGTCTTCACT CTTGACTTCT GGGCCCAATT CTCCCTTTTA CAAAGCCTTG
    ATTGAATCTG GCCTTGGCAC AGACTTTTCT CCTGATGTTG GATATAATGG CTACACGAGG
    GAGGCCTACT TTAGTGTCGG CCTCCAAGGG ATTGCGGAGA AAGACATTGA GACCGTCAGA
    AGCCTCATAG ACAGAACGAT TGATGAAGTA GTTGAGAAAG GATTTGAAGA TGATCGAATT
    GAGGCTTTAC TTCATAAAAT TGAAATACAG ATGAAACATC AGTCTACCAG CTTTGGGCTG
    ATGCTGACAT CATACATAGC TTCTTGCTGG AACCATGATG GGGACCCTGT GGAGCTCTTG
    AAGTTGGGAA ATCAGTTAGC TAAATTCAGA CAGTGCCTGC AGGAAAATCC AAAATTTTTG
    CAAGAAAAAG TAAAACAGTA TTTTAAGAAT AACCAGCATA AGCTGACTTT ATCGATGAGG
    CCAGATGACA AGTATCACGA GAAGCAGGCA CAGGTGGAAG CCACGAAGCT CAAGCAGAAG
    GTCGAGGCTC TGTCCCCCGG AGACAGGCAG CAGATCTACG AGAAAGGTCT AGAATTACGG
    AGTCAACAAA GCAAACCTCA AGATGCCTCT TGTCTGCCAG CGTTGAAAGT TTCCGATATT
    GAACCCACCA TACCTGTCAC AGAGTTGGAC GTGGTCCTGA CAGCTGGAGA TATCCCTGTT
    CAGTACTGCG CCCAGCCCAC CAATGGCATG GTGTATTTCC GGGCCTTCTC CAGCCTGAAC
    ACACTCCCCG AGGAGCTGAG GCCCTATGTG CCCCTCTTCT GCAGCGTCCT CACCAAGCTG
    GGCTGCGGCC TTCTTGACTA CCGGGAGCAG GCTCAGCAGA TAGAATTGAA GACCGGAGGG
    ATGAGTGCTT CTCCCCACGT GCTCCCCGAC GACTCACACA TGGACACCTA CGAGCAGGGT
    GTGCTTTTCT CCTCTCTCTG CCTGGATCGA AACCTGCCAG ACATGATGCA GCTATGGAGT
    GAAATATTTA ACAACCCGTG CTTTGAAGAA GAGGAGCACT TCAAGGTGCT GGTGAAGATG
    ACCGCCCAGG AGCTCGCCAA TGGAATTCCT GACTCTGGGC ACCTGTACGC ATCCATCAGG
    GCAGGCCGGA CCCTCACGCC CGCAGGGGAC CTGCAGGAGA CCTTCAGCGG GATGGATCAG
    GTGCGGCTGA TGAAGAGGAT TGCAGAAATG ACAGATATCA AACCCATCCT GAGGAAGCTC
    CCGCGTATCA AGAAACACTT GTTAAATGGT GATAATATGA GGTGTTCAGT GAATGCGACT
    CCTCAGCAGA TGCCTCAGAC AGAAAAAGCG GTCGAAGACT TCCTTAGAAG CATCGGTCGG
    AGTAAAAAGG AACGGAGGCC TGTGCGCCCA CACACGGTCG AGAAACCTGT GCCCAGCAGC
    TCTGGTGGAG ATGCCCACGT TCCCCATGGC TCCCAGGTCA TTAGGAAGCT GGTCATGGAA
    CCCACCTTCA AGCCCTGGCA GATGAAGACT CACTTCCTGA TGCCCTTCCC GGTGAATTAC
    GTGGGTGAAT GCATCCGAAC TGTCCCCTAC ACGGACCCAG ATCATGCCAG TCTTAAAATC
    CTTGCACGTT TGATGACTGC CAAATTCTTG CATACAGAAA TTCGAGAAAA AGGCGGTGCT
    TATGGTGGAG GCGCAAAACT CAGCCACAAT GGGATTTTCA CCCTTTACTC TTACAGGGAC
    CCAAATACAA TAGAGACGCT CCAGTCTTTT GGGAAGGCTG TCGACTGGGC TAAGTCTGGA
    AAATTCACAC AGCAAGACAT CGACGAAGCC AAACTTTCTG TCTTCTCAAC CGTAGATGCT
    CCTGTCGCTC CTTCAGACAA AGGAATGGAC CACTTCTTGT ACGGCCTCTC GGATGAGATG
    AAGCAGGCCC ACAGAGAGCA GCTCTTTGCT GTCAGCCACG ACAAGCTCCT GGCCGTGAGC
    GATAGATACC TCGGCACTGG GAAGAGCACA CACGGCCTGG CCATCCTCGG ACCCGAGAAC
    CCGAAAATTG CCAAGGACCC ATCCTGGATC ATCCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334656.1
    CDS582..3080
    Translation

    Target ORF information:

    RefSeq Version NM_001347727.2
    Organism Homo sapiens(human)
    Definition Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347727.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAGGGAG CGTTTACAGA CAATGAGAGG ATATTCTCCC AGCACCTTCA GAACAGACTT 
    CTTCCTGACC ACACGTACTC AGTGGTCTCC GGGGGTGACC CACTGTGCAT CCCGGAGCTT
    ACATGGGAGC AGCTTAAGCA GTTTCATGCC ACTCACTATC ACCCAAGCAA TGCTAGGTTC
    TTCACGTACG GTAATTTTCC ATTAGAACAG CATCTGAAAC AAATTCACGA GGAAGCACTG
    AGCAAATTCC AGAAAATTGA ACCAAGCACC GTGGTGCCAG CTCAGACACC CTGGGACAAG
    CCTAGGGAAT TCCAGATAAC ATGTGGCCCG GATTCATTTG CTACAGATCC CTCTAAACAA
    ACAACCATCA GCGTTAGCTT CCTCTTACCG GACATCACCG ACACATTTGA AGCCTTCACA
    TTAAGTCTTC TGTCTTCACT CTTGACTTCT GGGCCCAATT CTCCCTTTTA CAAAGCCTTG
    ATTGAATCTG GCCTTGGCAC AGACTTTTCT CCTGATGTTG GATATAATGG CTACACGAGG
    GAGGCCTACT TTAGTGTCGG CCTCCAAGGG ATTGCGGAGA AAGACATTGA GACCGTCAGA
    AGCCTCATAG ACAGAACGAT TGATGAAGTA GTTGAGAAAG GATTTGAAGA TGATCGAATT
    GAGGCTTTAC TTCATAAAAT TGAAATACAG ATGAAACATC AGTCTACCAG CTTTGGGCTG
    ATGCTGACAT CATACATAGC TTCTTGCTGG AACCATGATG GGGACCCTGT GGAGCTCTTG
    AAGTTGGGAA ATCAGTTAGC TAAATTCAGA CAGTGCCTGC AGGAAAATCC AAAATTTTTG
    CAAGAAAAAG TAAAACAGTA TTTTAAGAAT AACCAGCATA AGCTGACTTT ATCGATGAGG
    CCAGATGACA AGTATCACGA GAAGCAGGCA CAGGTGGAAG CCACGAAGCT CAAGCAGAAG
    GTCGAGGCTC TGTCCCCCGG AGACAGGCAG CAGATCTACG AGAAAGGTCT AGAATTACGG
    AGTCAACAAA GCAAACCTCA AGATGCCTCT TGTCTGCCAG CGTTGAAAGT TTCCGATATT
    GAACCCACCA TACCTGTCAC AGAGTTGGAC GTGGTCCTGA CAGCTGGAGA TATCCCTGTT
    CAGTACTGCG CCCAGCCCAC CAATGGCATG GTGTATTTCC GGGCCTTCTC CAGCCTGAAC
    ACACTCCCCG AGGAGCTGAG GCCCTATGTG CCCCTCTTCT GCAGCGTCCT CACCAAGCTG
    GGCTGCGGCC TTCTTGACTA CCGGGAGCAG GCTCAGCAGA TAGAATTGAA GACCGGAGGG
    ATGAGTGCTT CTCCCCACGT GCTCCCCGAC GACTCACACA TGGACACCTA CGAGCAGGGT
    GTGCTTTTCT CCTCTCTCTG CCTGGATCGA AACCTGCCAG ACATGATGCA GCTATGGAGT
    GAAATATTTA ACAACCCGTG CTTTGAAGAA GAGGAGCACT TCAAGGTGCT GGTGAAGATG
    ACCGCCCAGG AGCTCGCCAA TGGAATTCCT GACTCTGGGC ACCTGTACGC ATCCATCAGG
    GCAGGCCGGA CCCTCACGCC CGCAGGGGAC CTGCAGGAGA CCTTCAGCGG GATGGATCAG
    GTGCGGCTGA TGAAGAGGAT TGCAGAAATG ACAGATATCA AACCCATCCT GAGGAAGCTC
    CCGCGTATCA AGAAACACTT GTTAAATGGT GATAATATGA GGTGTTCAGT GAATGCGACT
    CCTCAGCAGA TGCCTCAGAC AGAAAAAGCG GTCGAAGACT TCCTTAGAAG CATCGGTCGG
    AGTAAAAAGG AACGGAGGCC TGTGCGCCCA CACACGGTCG AGAAACCTGT GCCCAGCAGC
    TCTGGTGGAG ATGCCCACGT TCCCCATGGC TCCCAGGTCA TTAGGAAGCT GGTCATGGAA
    CCCACCTTCA AGCCCTGGCA GATGAAGACT CACTTCCTGA TGCCCTTCCC GGTGAATTAC
    GTGGGTGAAT GCATCCGAAC TGTCCCCTAC ACGGACCCAG ATCATGCCAG TCTTAAAATC
    CTTGCACGTT TGATGACTGC CAAATTCTTG CATACAGAAA TTCGAGAAAA AGGCGGTGCT
    TATGGTGGAG GCGCAAAACT CAGCCACAAT GGGATTTTCA CCCTTTACTC TTACAGGGAC
    CCAAATACAA TAGAGACGCT CCAGTCTTTT GGGAAGGCTG TCGACTGGGC TAAGTCTGGA
    AAATTCACAC AGCAAGACAT CGACGAAGCC AAACTTTCTG TCTTCTCAAC CGTAGATGCT
    CCTGTCGCTC CTTCAGACAA AGGAATGGAC CACTTCTTGT ACGGCCTCTC GGATGAGATG
    AAGCAGGCCC ACAGAGAGCA GCTCTTTGCT GTCAGCCACG ACAAGCTCCT GGCCGTGAGC
    GATAGATACC TCGGCACTGG GAAGAGCACA CACGGCCTGG CCATCCTCGG ACCCGAGAAC
    CCGAAAATTG CCAAGGACCC ATCCTGGATC ATCCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101521
    Clone ID Related Accession (Same CDS sequence) NM_001347727.2 , NM_001347727.1
    Accession Version NM_001347727.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2499bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product presequence protease, mitochondrial isoform 6
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL451164.13. On Dec 13, 2016 this sequence version replaced XM_017015472.1. Transcript Variant: This variant (6) encodes isoform 6, which likely does not localize to the mitochondrion. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.56546.1, SRR1803613.168203.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAGGGAG CGTTTACAGA CAATGAGAGG ATATTCTCCC AGCACCTTCA GAACAGACTT 
    CTTCCTGACC ACACGTACTC AGTGGTCTCC GGGGGTGACC CACTGTGCAT CCCGGAGCTT
    ACATGGGAGC AGCTTAAGCA GTTTCATGCC ACTCACTATC ACCCAAGCAA TGCTAGGTTC
    TTCACGTACG GTAATTTTCC ATTAGAACAG CATCTGAAAC AAATTCACGA GGAAGCACTG
    AGCAAATTCC AGAAAATTGA ACCAAGCACC GTGGTGCCAG CTCAGACACC CTGGGACAAG
    CCTAGGGAAT TCCAGATAAC ATGTGGCCCG GATTCATTTG CTACAGATCC CTCTAAACAA
    ACAACCATCA GCGTTAGCTT CCTCTTACCG GACATCACCG ACACATTTGA AGCCTTCACA
    TTAAGTCTTC TGTCTTCACT CTTGACTTCT GGGCCCAATT CTCCCTTTTA CAAAGCCTTG
    ATTGAATCTG GCCTTGGCAC AGACTTTTCT CCTGATGTTG GATATAATGG CTACACGAGG
    GAGGCCTACT TTAGTGTCGG CCTCCAAGGG ATTGCGGAGA AAGACATTGA GACCGTCAGA
    AGCCTCATAG ACAGAACGAT TGATGAAGTA GTTGAGAAAG GATTTGAAGA TGATCGAATT
    GAGGCTTTAC TTCATAAAAT TGAAATACAG ATGAAACATC AGTCTACCAG CTTTGGGCTG
    ATGCTGACAT CATACATAGC TTCTTGCTGG AACCATGATG GGGACCCTGT GGAGCTCTTG
    AAGTTGGGAA ATCAGTTAGC TAAATTCAGA CAGTGCCTGC AGGAAAATCC AAAATTTTTG
    CAAGAAAAAG TAAAACAGTA TTTTAAGAAT AACCAGCATA AGCTGACTTT ATCGATGAGG
    CCAGATGACA AGTATCACGA GAAGCAGGCA CAGGTGGAAG CCACGAAGCT CAAGCAGAAG
    GTCGAGGCTC TGTCCCCCGG AGACAGGCAG CAGATCTACG AGAAAGGTCT AGAATTACGG
    AGTCAACAAA GCAAACCTCA AGATGCCTCT TGTCTGCCAG CGTTGAAAGT TTCCGATATT
    GAACCCACCA TACCTGTCAC AGAGTTGGAC GTGGTCCTGA CAGCTGGAGA TATCCCTGTT
    CAGTACTGCG CCCAGCCCAC CAATGGCATG GTGTATTTCC GGGCCTTCTC CAGCCTGAAC
    ACACTCCCCG AGGAGCTGAG GCCCTATGTG CCCCTCTTCT GCAGCGTCCT CACCAAGCTG
    GGCTGCGGCC TTCTTGACTA CCGGGAGCAG GCTCAGCAGA TAGAATTGAA GACCGGAGGG
    ATGAGTGCTT CTCCCCACGT GCTCCCCGAC GACTCACACA TGGACACCTA CGAGCAGGGT
    GTGCTTTTCT CCTCTCTCTG CCTGGATCGA AACCTGCCAG ACATGATGCA GCTATGGAGT
    GAAATATTTA ACAACCCGTG CTTTGAAGAA GAGGAGCACT TCAAGGTGCT GGTGAAGATG
    ACCGCCCAGG AGCTCGCCAA TGGAATTCCT GACTCTGGGC ACCTGTACGC ATCCATCAGG
    GCAGGCCGGA CCCTCACGCC CGCAGGGGAC CTGCAGGAGA CCTTCAGCGG GATGGATCAG
    GTGCGGCTGA TGAAGAGGAT TGCAGAAATG ACAGATATCA AACCCATCCT GAGGAAGCTC
    CCGCGTATCA AGAAACACTT GTTAAATGGT GATAATATGA GGTGTTCAGT GAATGCGACT
    CCTCAGCAGA TGCCTCAGAC AGAAAAAGCG GTCGAAGACT TCCTTAGAAG CATCGGTCGG
    AGTAAAAAGG AACGGAGGCC TGTGCGCCCA CACACGGTCG AGAAACCTGT GCCCAGCAGC
    TCTGGTGGAG ATGCCCACGT TCCCCATGGC TCCCAGGTCA TTAGGAAGCT GGTCATGGAA
    CCCACCTTCA AGCCCTGGCA GATGAAGACT CACTTCCTGA TGCCCTTCCC GGTGAATTAC
    GTGGGTGAAT GCATCCGAAC TGTCCCCTAC ACGGACCCAG ATCATGCCAG TCTTAAAATC
    CTTGCACGTT TGATGACTGC CAAATTCTTG CATACAGAAA TTCGAGAAAA AGGCGGTGCT
    TATGGTGGAG GCGCAAAACT CAGCCACAAT GGGATTTTCA CCCTTTACTC TTACAGGGAC
    CCAAATACAA TAGAGACGCT CCAGTCTTTT GGGAAGGCTG TCGACTGGGC TAAGTCTGGA
    AAATTCACAC AGCAAGACAT CGACGAAGCC AAACTTTCTG TCTTCTCAAC CGTAGATGCT
    CCTGTCGCTC CTTCAGACAA AGGAATGGAC CACTTCTTGT ACGGCCTCTC GGATGAGATG
    AAGCAGGCCC ACAGAGAGCA GCTCTTTGCT GTCAGCCACG ACAAGCTCCT GGCCGTGAGC
    GATAGATACC TCGGCACTGG GAAGAGCACA CACGGCCTGG CCATCCTCGG ACCCGAGAAC
    CCGAAAATTG CCAAGGACCC ATCCTGGATC ATCCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334656.1
    CDS641..3139
    Translation

    Target ORF information:

    RefSeq Version NM_001347727.1
    Organism Homo sapiens(human)
    Definition Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347727.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAGGGAG CGTTTACAGA CAATGAGAGG ATATTCTCCC AGCACCTTCA GAACAGACTT 
    CTTCCTGACC ACACGTACTC AGTGGTCTCC GGGGGTGACC CACTGTGCAT CCCGGAGCTT
    ACATGGGAGC AGCTTAAGCA GTTTCATGCC ACTCACTATC ACCCAAGCAA TGCTAGGTTC
    TTCACGTACG GTAATTTTCC ATTAGAACAG CATCTGAAAC AAATTCACGA GGAAGCACTG
    AGCAAATTCC AGAAAATTGA ACCAAGCACC GTGGTGCCAG CTCAGACACC CTGGGACAAG
    CCTAGGGAAT TCCAGATAAC ATGTGGCCCG GATTCATTTG CTACAGATCC CTCTAAACAA
    ACAACCATCA GCGTTAGCTT CCTCTTACCG GACATCACCG ACACATTTGA AGCCTTCACA
    TTAAGTCTTC TGTCTTCACT CTTGACTTCT GGGCCCAATT CTCCCTTTTA CAAAGCCTTG
    ATTGAATCTG GCCTTGGCAC AGACTTTTCT CCTGATGTTG GATATAATGG CTACACGAGG
    GAGGCCTACT TTAGTGTCGG CCTCCAAGGG ATTGCGGAGA AAGACATTGA GACCGTCAGA
    AGCCTCATAG ACAGAACGAT TGATGAAGTA GTTGAGAAAG GATTTGAAGA TGATCGAATT
    GAGGCTTTAC TTCATAAAAT TGAAATACAG ATGAAACATC AGTCTACCAG CTTTGGGCTG
    ATGCTGACAT CATACATAGC TTCTTGCTGG AACCATGATG GGGACCCTGT GGAGCTCTTG
    AAGTTGGGAA ATCAGTTAGC TAAATTCAGA CAGTGCCTGC AGGAAAATCC AAAATTTTTG
    CAAGAAAAAG TAAAACAGTA TTTTAAGAAT AACCAGCATA AGCTGACTTT ATCGATGAGG
    CCAGATGACA AGTATCACGA GAAGCAGGCA CAGGTGGAAG CCACGAAGCT CAAGCAGAAG
    GTCGAGGCTC TGTCCCCCGG AGACAGGCAG CAGATCTACG AGAAAGGTCT AGAATTACGG
    AGTCAACAAA GCAAACCTCA AGATGCCTCT TGTCTGCCAG CGTTGAAAGT TTCCGATATT
    GAACCCACCA TACCTGTCAC AGAGTTGGAC GTGGTCCTGA CAGCTGGAGA TATCCCTGTT
    CAGTACTGCG CCCAGCCCAC CAATGGCATG GTGTATTTCC GGGCCTTCTC CAGCCTGAAC
    ACACTCCCCG AGGAGCTGAG GCCCTATGTG CCCCTCTTCT GCAGCGTCCT CACCAAGCTG
    GGCTGCGGCC TTCTTGACTA CCGGGAGCAG GCTCAGCAGA TAGAATTGAA GACCGGAGGG
    ATGAGTGCTT CTCCCCACGT GCTCCCCGAC GACTCACACA TGGACACCTA CGAGCAGGGT
    GTGCTTTTCT CCTCTCTCTG CCTGGATCGA AACCTGCCAG ACATGATGCA GCTATGGAGT
    GAAATATTTA ACAACCCGTG CTTTGAAGAA GAGGAGCACT TCAAGGTGCT GGTGAAGATG
    ACCGCCCAGG AGCTCGCCAA TGGAATTCCT GACTCTGGGC ACCTGTACGC ATCCATCAGG
    GCAGGCCGGA CCCTCACGCC CGCAGGGGAC CTGCAGGAGA CCTTCAGCGG GATGGATCAG
    GTGCGGCTGA TGAAGAGGAT TGCAGAAATG ACAGATATCA AACCCATCCT GAGGAAGCTC
    CCGCGTATCA AGAAACACTT GTTAAATGGT GATAATATGA GGTGTTCAGT GAATGCGACT
    CCTCAGCAGA TGCCTCAGAC AGAAAAAGCG GTCGAAGACT TCCTTAGAAG CATCGGTCGG
    AGTAAAAAGG AACGGAGGCC TGTGCGCCCA CACACGGTCG AGAAACCTGT GCCCAGCAGC
    TCTGGTGGAG ATGCCCACGT TCCCCATGGC TCCCAGGTCA TTAGGAAGCT GGTCATGGAA
    CCCACCTTCA AGCCCTGGCA GATGAAGACT CACTTCCTGA TGCCCTTCCC GGTGAATTAC
    GTGGGTGAAT GCATCCGAAC TGTCCCCTAC ACGGACCCAG ATCATGCCAG TCTTAAAATC
    CTTGCACGTT TGATGACTGC CAAATTCTTG CATACAGAAA TTCGAGAAAA AGGCGGTGCT
    TATGGTGGAG GCGCAAAACT CAGCCACAAT GGGATTTTCA CCCTTTACTC TTACAGGGAC
    CCAAATACAA TAGAGACGCT CCAGTCTTTT GGGAAGGCTG TCGACTGGGC TAAGTCTGGA
    AAATTCACAC AGCAAGACAT CGACGAAGCC AAACTTTCTG TCTTCTCAAC CGTAGATGCT
    CCTGTCGCTC CTTCAGACAA AGGAATGGAC CACTTCTTGT ACGGCCTCTC GGATGAGATG
    AAGCAGGCCC ACAGAGAGCA GCTCTTTGCT GTCAGCCACG ACAAGCTCCT GGCCGTGAGC
    GATAGATACC TCGGCACTGG GAAGAGCACA CACGGCCTGG CCATCCTCGG ACCCGAGAAC
    CCGAAAATTG CCAAGGACCC ATCCTGGATC ATCCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101524
    Clone ID Related Accession (Same CDS sequence) NM_001347728.1 , NM_001347728.2
    Accession Version NM_001347728.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1809bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product presequence protease, mitochondrial isoform 7
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL451164.13. Transcript Variant: This variant (7) encodes isoform 7, which likely does not localize to the mitochondrion. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.12954.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAAACATC AGTCTACCAG CTTTGGGCTG ATGCTGACAT CATACATAGC TTCTTGCTGG 
    AACCATGATG GGGACCCTGT GGAGCTCTTG AAGTTGGGAA ATCAGTTAGC TAAATTCAGA
    CAGTGCCTGC AGGAAAATCC AAAATTTTTG CAAGAAAAAG TAAAACAGTA TTTTAAGAAT
    AACCAGCATA AGCTGACTTT ATCGATGAGG CCAGATGACA AGTATCACGA GAAGCAGGCA
    CAGGTGGAAG CCACGAAGCT CAAGCAGAAG GTCGAGGCTC TGTCCCCCGG AGACAGGCAG
    CAGATCTACG AGAAAGGTCT AGAATTACGG AGTCAACAAA GCAAACCTCA AGATGCCTCT
    TGTCTGCCAG CGTTGAAAGT TTCCGATATT GAACCCACCA TACCTGTCAC AGAGTTGGAC
    GTGGTCCTGA CAGCTGGAGA TATCCCTGTT CAGTACTGCG CCCAGCCCAC CAATGGCATG
    GTGTATTTCC GGGCCTTCTC CAGCCTGAAC ACACTCCCCG AGGAGCTGAG GCCCTATGTG
    CCCCTCTTCT GCAGCGTCCT CACCAAGCTG GGCTGCGGCC TTCTTGACTA CCGGGAGCAG
    GCTCAGCAGA TAGAATTGAA GACCGGAGGG ATGAGTGCTT CTCCCCACGT GCTCCCCGAC
    GACTCACACA TGGACACCTA CGAGCAGGGT GTGCTTTTCT CCTCTCTCTG CCTGGATCGA
    AACCTGCCAG ACATGATGCA GCTATGGAGT GAAATATTTA ACAACCCGTG CTTTGAAGAA
    GAGGAGCACT TCAAGGTGCT GGTGAAGATG ACCGCCCAGG AGCTCGCCAA TGGAATTCCT
    GACTCTGGGC ACCTGTACGC ATCCATCAGG GCAGGCCGGA CCCTCACGCC CGCAGGGGAC
    CTGCAGGAGA CCTTCAGCGG GATGGATCAG GTGCGGCTGA TGAAGAGGAT TGCAGAAATG
    ACAGATATCA AACCCATCCT GAGGAAGCTC CCGCGTATCA AGAAACACTT GTTAAATGGT
    GATAATATGA GGTGTTCAGT GAATGCGACT CCTCAGCAGA TGCCTCAGAC AGAAAAAGCG
    GTCGAAGACT TCCTTAGAAG CATCGGTCGG AGTAAAAAGG AACGGAGGCC TGTGCGCCCA
    CACACGGTCG AGAAACCTGT GCCCAGCAGC TCTGGTGGAG ATGCCCACGT TCCCCATGGC
    TCCCAGGTCA TTAGGAAGCT GGTCATGGAA CCCACCTTCA AGCCCTGGCA GATGAAGACT
    CACTTCCTGA TGCCCTTCCC GGTGAATTAC GTGGGTGAAT GCATCCGAAC TGTCCCCTAC
    ACGGACCCAG ATCATGCCAG TCTTAAAATC CTTGCACGTT TGATGACTGC CAAATTCTTG
    CATACAGAAA TTCGAGAAAA AGGCGGTGCT TATGGTGGAG GCGCAAAACT CAGCCACAAT
    GGGATTTTCA CCCTTTACTC TTACAGGGAC CCAAATACAA TAGAGACGCT CCAGTCTTTT
    GGGAAGGCTG TCGACTGGGC TAAGTCTGGA AAATTCACAC AGCAAGACAT CGACGAAGCC
    AAACTTTCTG TCTTCTCAAC CGTAGATGCT CCTGTCGCTC CTTCAGACAA AGGAATGGAC
    CACTTCTTGT ACGGCCTCTC GGATGAGATG AAGCAGGCCC ACAGAGAGCA GCTCTTTGCT
    GTCAGCCACG ACAAGCTCCT GGCCGTGAGC GATAGATACC TCGGCACTGG GAAGAGCACA
    CACGGCCTGG CCATCCTCGG ACCCGAGAAC CCGAAAATTG CCAAGGACCC ATCCTGGATC
    ATCCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334657.1
    CDS1370..3178
    Translation

    Target ORF information:

    RefSeq Version NM_001347728.1
    Organism Homo sapiens(human)
    Definition Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347728.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAAACATC AGTCTACCAG CTTTGGGCTG ATGCTGACAT CATACATAGC TTCTTGCTGG 
    AACCATGATG GGGACCCTGT GGAGCTCTTG AAGTTGGGAA ATCAGTTAGC TAAATTCAGA
    CAGTGCCTGC AGGAAAATCC AAAATTTTTG CAAGAAAAAG TAAAACAGTA TTTTAAGAAT
    AACCAGCATA AGCTGACTTT ATCGATGAGG CCAGATGACA AGTATCACGA GAAGCAGGCA
    CAGGTGGAAG CCACGAAGCT CAAGCAGAAG GTCGAGGCTC TGTCCCCCGG AGACAGGCAG
    CAGATCTACG AGAAAGGTCT AGAATTACGG AGTCAACAAA GCAAACCTCA AGATGCCTCT
    TGTCTGCCAG CGTTGAAAGT TTCCGATATT GAACCCACCA TACCTGTCAC AGAGTTGGAC
    GTGGTCCTGA CAGCTGGAGA TATCCCTGTT CAGTACTGCG CCCAGCCCAC CAATGGCATG
    GTGTATTTCC GGGCCTTCTC CAGCCTGAAC ACACTCCCCG AGGAGCTGAG GCCCTATGTG
    CCCCTCTTCT GCAGCGTCCT CACCAAGCTG GGCTGCGGCC TTCTTGACTA CCGGGAGCAG
    GCTCAGCAGA TAGAATTGAA GACCGGAGGG ATGAGTGCTT CTCCCCACGT GCTCCCCGAC
    GACTCACACA TGGACACCTA CGAGCAGGGT GTGCTTTTCT CCTCTCTCTG CCTGGATCGA
    AACCTGCCAG ACATGATGCA GCTATGGAGT GAAATATTTA ACAACCCGTG CTTTGAAGAA
    GAGGAGCACT TCAAGGTGCT GGTGAAGATG ACCGCCCAGG AGCTCGCCAA TGGAATTCCT
    GACTCTGGGC ACCTGTACGC ATCCATCAGG GCAGGCCGGA CCCTCACGCC CGCAGGGGAC
    CTGCAGGAGA CCTTCAGCGG GATGGATCAG GTGCGGCTGA TGAAGAGGAT TGCAGAAATG
    ACAGATATCA AACCCATCCT GAGGAAGCTC CCGCGTATCA AGAAACACTT GTTAAATGGT
    GATAATATGA GGTGTTCAGT GAATGCGACT CCTCAGCAGA TGCCTCAGAC AGAAAAAGCG
    GTCGAAGACT TCCTTAGAAG CATCGGTCGG AGTAAAAAGG AACGGAGGCC TGTGCGCCCA
    CACACGGTCG AGAAACCTGT GCCCAGCAGC TCTGGTGGAG ATGCCCACGT TCCCCATGGC
    TCCCAGGTCA TTAGGAAGCT GGTCATGGAA CCCACCTTCA AGCCCTGGCA GATGAAGACT
    CACTTCCTGA TGCCCTTCCC GGTGAATTAC GTGGGTGAAT GCATCCGAAC TGTCCCCTAC
    ACGGACCCAG ATCATGCCAG TCTTAAAATC CTTGCACGTT TGATGACTGC CAAATTCTTG
    CATACAGAAA TTCGAGAAAA AGGCGGTGCT TATGGTGGAG GCGCAAAACT CAGCCACAAT
    GGGATTTTCA CCCTTTACTC TTACAGGGAC CCAAATACAA TAGAGACGCT CCAGTCTTTT
    GGGAAGGCTG TCGACTGGGC TAAGTCTGGA AAATTCACAC AGCAAGACAT CGACGAAGCC
    AAACTTTCTG TCTTCTCAAC CGTAGATGCT CCTGTCGCTC CTTCAGACAA AGGAATGGAC
    CACTTCTTGT ACGGCCTCTC GGATGAGATG AAGCAGGCCC ACAGAGAGCA GCTCTTTGCT
    GTCAGCCACG ACAAGCTCCT GGCCGTGAGC GATAGATACC TCGGCACTGG GAAGAGCACA
    CACGGCCTGG CCATCCTCGG ACCCGAGAAC CCGAAAATTG CCAAGGACCC ATCCTGGATC
    ATCCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101524
    Clone ID Related Accession (Same CDS sequence) NM_001347728.1 , NM_001347728.2
    Accession Version NM_001347728.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1809bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-25
    Organism Homo sapiens(human)
    Product presequence protease, mitochondrial isoform 7
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL451164.13. On May 31, 2019 this sequence version replaced NM_001347728.1. Transcript Variant: This variant (7) encodes isoform 7, which likely does not localize to the mitochondrion. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.12954.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAAACATC AGTCTACCAG CTTTGGGCTG ATGCTGACAT CATACATAGC TTCTTGCTGG 
    AACCATGATG GGGACCCTGT GGAGCTCTTG AAGTTGGGAA ATCAGTTAGC TAAATTCAGA
    CAGTGCCTGC AGGAAAATCC AAAATTTTTG CAAGAAAAAG TAAAACAGTA TTTTAAGAAT
    AACCAGCATA AGCTGACTTT ATCGATGAGG CCAGATGACA AGTATCACGA GAAGCAGGCA
    CAGGTGGAAG CCACGAAGCT CAAGCAGAAG GTCGAGGCTC TGTCCCCCGG AGACAGGCAG
    CAGATCTACG AGAAAGGTCT AGAATTACGG AGTCAACAAA GCAAACCTCA AGATGCCTCT
    TGTCTGCCAG CGTTGAAAGT TTCCGATATT GAACCCACCA TACCTGTCAC AGAGTTGGAC
    GTGGTCCTGA CAGCTGGAGA TATCCCTGTT CAGTACTGCG CCCAGCCCAC CAATGGCATG
    GTGTATTTCC GGGCCTTCTC CAGCCTGAAC ACACTCCCCG AGGAGCTGAG GCCCTATGTG
    CCCCTCTTCT GCAGCGTCCT CACCAAGCTG GGCTGCGGCC TTCTTGACTA CCGGGAGCAG
    GCTCAGCAGA TAGAATTGAA GACCGGAGGG ATGAGTGCTT CTCCCCACGT GCTCCCCGAC
    GACTCACACA TGGACACCTA CGAGCAGGGT GTGCTTTTCT CCTCTCTCTG CCTGGATCGA
    AACCTGCCAG ACATGATGCA GCTATGGAGT GAAATATTTA ACAACCCGTG CTTTGAAGAA
    GAGGAGCACT TCAAGGTGCT GGTGAAGATG ACCGCCCAGG AGCTCGCCAA TGGAATTCCT
    GACTCTGGGC ACCTGTACGC ATCCATCAGG GCAGGCCGGA CCCTCACGCC CGCAGGGGAC
    CTGCAGGAGA CCTTCAGCGG GATGGATCAG GTGCGGCTGA TGAAGAGGAT TGCAGAAATG
    ACAGATATCA AACCCATCCT GAGGAAGCTC CCGCGTATCA AGAAACACTT GTTAAATGGT
    GATAATATGA GGTGTTCAGT GAATGCGACT CCTCAGCAGA TGCCTCAGAC AGAAAAAGCG
    GTCGAAGACT TCCTTAGAAG CATCGGTCGG AGTAAAAAGG AACGGAGGCC TGTGCGCCCA
    CACACGGTCG AGAAACCTGT GCCCAGCAGC TCTGGTGGAG ATGCCCACGT TCCCCATGGC
    TCCCAGGTCA TTAGGAAGCT GGTCATGGAA CCCACCTTCA AGCCCTGGCA GATGAAGACT
    CACTTCCTGA TGCCCTTCCC GGTGAATTAC GTGGGTGAAT GCATCCGAAC TGTCCCCTAC
    ACGGACCCAG ATCATGCCAG TCTTAAAATC CTTGCACGTT TGATGACTGC CAAATTCTTG
    CATACAGAAA TTCGAGAAAA AGGCGGTGCT TATGGTGGAG GCGCAAAACT CAGCCACAAT
    GGGATTTTCA CCCTTTACTC TTACAGGGAC CCAAATACAA TAGAGACGCT CCAGTCTTTT
    GGGAAGGCTG TCGACTGGGC TAAGTCTGGA AAATTCACAC AGCAAGACAT CGACGAAGCC
    AAACTTTCTG TCTTCTCAAC CGTAGATGCT CCTGTCGCTC CTTCAGACAA AGGAATGGAC
    CACTTCTTGT ACGGCCTCTC GGATGAGATG AAGCAGGCCC ACAGAGAGCA GCTCTTTGCT
    GTCAGCCACG ACAAGCTCCT GGCCGTGAGC GATAGATACC TCGGCACTGG GAAGAGCACA
    CACGGCCTGG CCATCCTCGG ACCCGAGAAC CCGAAAATTG CCAAGGACCC ATCCTGGATC
    ATCCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334657.1
    CDS1311..3119
    Translation

    Target ORF information:

    RefSeq Version NM_001347728.2
    Organism Homo sapiens(human)
    Definition Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347728.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAAACATC AGTCTACCAG CTTTGGGCTG ATGCTGACAT CATACATAGC TTCTTGCTGG 
    AACCATGATG GGGACCCTGT GGAGCTCTTG AAGTTGGGAA ATCAGTTAGC TAAATTCAGA
    CAGTGCCTGC AGGAAAATCC AAAATTTTTG CAAGAAAAAG TAAAACAGTA TTTTAAGAAT
    AACCAGCATA AGCTGACTTT ATCGATGAGG CCAGATGACA AGTATCACGA GAAGCAGGCA
    CAGGTGGAAG CCACGAAGCT CAAGCAGAAG GTCGAGGCTC TGTCCCCCGG AGACAGGCAG
    CAGATCTACG AGAAAGGTCT AGAATTACGG AGTCAACAAA GCAAACCTCA AGATGCCTCT
    TGTCTGCCAG CGTTGAAAGT TTCCGATATT GAACCCACCA TACCTGTCAC AGAGTTGGAC
    GTGGTCCTGA CAGCTGGAGA TATCCCTGTT CAGTACTGCG CCCAGCCCAC CAATGGCATG
    GTGTATTTCC GGGCCTTCTC CAGCCTGAAC ACACTCCCCG AGGAGCTGAG GCCCTATGTG
    CCCCTCTTCT GCAGCGTCCT CACCAAGCTG GGCTGCGGCC TTCTTGACTA CCGGGAGCAG
    GCTCAGCAGA TAGAATTGAA GACCGGAGGG ATGAGTGCTT CTCCCCACGT GCTCCCCGAC
    GACTCACACA TGGACACCTA CGAGCAGGGT GTGCTTTTCT CCTCTCTCTG CCTGGATCGA
    AACCTGCCAG ACATGATGCA GCTATGGAGT GAAATATTTA ACAACCCGTG CTTTGAAGAA
    GAGGAGCACT TCAAGGTGCT GGTGAAGATG ACCGCCCAGG AGCTCGCCAA TGGAATTCCT
    GACTCTGGGC ACCTGTACGC ATCCATCAGG GCAGGCCGGA CCCTCACGCC CGCAGGGGAC
    CTGCAGGAGA CCTTCAGCGG GATGGATCAG GTGCGGCTGA TGAAGAGGAT TGCAGAAATG
    ACAGATATCA AACCCATCCT GAGGAAGCTC CCGCGTATCA AGAAACACTT GTTAAATGGT
    GATAATATGA GGTGTTCAGT GAATGCGACT CCTCAGCAGA TGCCTCAGAC AGAAAAAGCG
    GTCGAAGACT TCCTTAGAAG CATCGGTCGG AGTAAAAAGG AACGGAGGCC TGTGCGCCCA
    CACACGGTCG AGAAACCTGT GCCCAGCAGC TCTGGTGGAG ATGCCCACGT TCCCCATGGC
    TCCCAGGTCA TTAGGAAGCT GGTCATGGAA CCCACCTTCA AGCCCTGGCA GATGAAGACT
    CACTTCCTGA TGCCCTTCCC GGTGAATTAC GTGGGTGAAT GCATCCGAAC TGTCCCCTAC
    ACGGACCCAG ATCATGCCAG TCTTAAAATC CTTGCACGTT TGATGACTGC CAAATTCTTG
    CATACAGAAA TTCGAGAAAA AGGCGGTGCT TATGGTGGAG GCGCAAAACT CAGCCACAAT
    GGGATTTTCA CCCTTTACTC TTACAGGGAC CCAAATACAA TAGAGACGCT CCAGTCTTTT
    GGGAAGGCTG TCGACTGGGC TAAGTCTGGA AAATTCACAC AGCAAGACAT CGACGAAGCC
    AAACTTTCTG TCTTCTCAAC CGTAGATGCT CCTGTCGCTC CTTCAGACAA AGGAATGGAC
    CACTTCTTGT ACGGCCTCTC GGATGAGATG AAGCAGGCCC ACAGAGAGCA GCTCTTTGCT
    GTCAGCCACG ACAAGCTCCT GGCCGTGAGC GATAGATACC TCGGCACTGG GAAGAGCACA
    CACGGCCTGG CCATCCTCGG ACCCGAGAAC CCGAAAATTG CCAAGGACCC ATCCTGGATC
    ATCCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101531
    Clone ID Related Accession (Same CDS sequence) NM_001347729.1
    Accession Version NM_001347729.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3090bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-21
    Organism Homo sapiens(human)
    Product presequence protease, mitochondrial isoform 8
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL451164.13. On Dec 13, 2016 this sequence version replaced XM_017015470.1. Transcript Variant: This variant (8) encodes isoform 8, which likely does not localize to the mitochondrion. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.58390.1, SRR1803611.94169.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGTAATCC ACGAGCCCGT GTTTAATATA AGACATGCAC ACCACAGAGC GTGGCGATGG 
    AACAGTAACC GGGCTTGTGA GAGGGCTCTG CAGTATAAAC TAGGAGACAA GATCCATGGA
    TTCACCGTAA ACCAGGTGAC ATCTGTTCCC GAGCTGTTCC TGACTGCAGT GAAGCTCACC
    CATGATGACA CAGGAGCCAG GTATTTACAC CTGGCCAGAG AAGACACGAA TAATCTGTTC
    AGCGTGCAGT TCCGTACTAC TCCCATGGAC AGTACTGGTG TTCCTCACAT TCTTGAGCAT
    ACCGTCCTTT GTGGGTCTCA GAAATATCCG TGCAGAGACC CTTTCTTCAA AATGTTGAAC
    CGGTCCCTCT CCACGTTCAT GAACGCCTTC ACAGCTAGTG ATTATACTCT GTATCCATTT
    TCCACACAAA ATCCCAAGGA CTTTCAGAAT CTCCTCTCGG TGTATTTGGA TGCCACCTTT
    TTCCCATGTT TACGCGAGCT GGATTTCTGG CAGGAAGGAT GGCGGCTGGA ACATGAGAAT
    CCGAGCGACC CCCAGACGCC CTTGGTCTTT AAAGGAGTCG TCTTTAATGA GATGAAGGGA
    GCGTTTACAG ACAATGAGAG GATATTCTCC CAGCACCTTC AGAACAGACT TCTTCCTGAC
    CACACGTACT CAGTGGTCTC CGGGGGTGAC CCACTGTGCA TCCCGGAGCT TACATGGGAG
    CAGCTTAAGC AGTTTCATGC CACTCACTAT CACCCAAGCA ATGCTAGGTT CTTCACGTAC
    GGTAATTTTC CATTAGAACA GCATCTGAAA CAAATTCACG AGGAAGCACT GAGCAAATTC
    CAGAAAATTG AACCAAGCAC CGTGGTGCCA GCTCAGACAC CCTGGGACAA GCCTAGGGAA
    TTCCAGATAA CATGTGGCCC GGATTCATTT GCTACAGATC CCTCTAAACA AACAACCATC
    AGCGTTAGCT TCCTCTTACC GGACATCACC GACACATTTG AAGCCTTCAC ATTAAGTCTT
    CTGTCTTCAC TCTTGACTTC TGGGCCCAAT TCTCCCTTTT ACAAAGCCTT GATTGAATCT
    GGCCTTGGCA CAGACTTTTC TCCTGATGTT GGATATAATG GCTACACGAG GGAGGCCTAC
    TTTAGTGTCG GCCTCCAAGG GATTGCGGAG AAAGACATTG AGACCGTCAG AAGCCTCATA
    GACAGAACGA TTGATGAAGT AGTTGAGAAA GGATTTGAAG ATGATCGAAT TGAGGCTTTA
    CTTCATAAAA TTGAAATACA GATGAAACAT CAGTCTACCA GCTTTGGGCT GATGCTGACA
    TCATACATAG CTTCTTGCTG GAACCATGAT GGGGACCCTG TGGAGCTCTT GAAGTTGGGA
    AATCAGTTAG CTAAATTCAG ACAGTGCCTG CAGGAAAATC CAAAATTTTT GCAAGAAAAA
    GTAAAACAGT ATTTTAAGAA TAACCAGCAT AAGCTGACTT TATCGATGAG GCCAGATGAC
    AAGTATCACG AGAAGCAGGC ACAGGTGGAA GCCACGAAGC TCAAGCAGAA GGTCGAGGCT
    CTGTCCCCCG GAGACAGGCA GCAGATCTAC GAGAAAGGTC TAGAATTACG GAGTCAACAA
    AGCAAACCTC AAGATGCCTC TTGTCTGCCA GCGTTGAAAG TTTCCGATAT TGAACCCACC
    ATACCTGTCA CAGAGTTGGA CGTGGTCCTG ACAGCTGGAG ATATCCCTGT TCAGTACTGC
    GCCCAGCCCA CCAATGGCAT GGTGTATTTC CGGGCCTTCT CCAGCCTGAA CACACTCCCC
    GAGGAGCTGA GGCCCTATGT GCCCCTCTTC TGCAGCGTCC TCACCAAGCT GGGCTGCGGC
    CTTCTTGACT ACCGGGAGCA GGCTCAGCAG ATAGAATTGA AGACCGGAGG GATGAGTGCT
    TCTCCCCACG TGCTCCCCGA CGACTCACAC ATGGACACCT ACGAGCAGGG TGTGCTTTTC
    TCCTCTCTCT GCCTGGATCG AAACCTGCCA GACATGATGC AGCTATGGAG TGAAATATTT
    AACAACCCGT GCTTTGAAGA AGAGGAGCAC TTCAAGGTGC TGGTGAAGAT GACCGCCCAG
    GAGCTCGCCA ATGGAATTCC TGACTCTGGG CACCTGTACG CATCCATCAG GGCAGGCCGG
    ACCCTCACGC CCGCAGGGGA CCTGCAGGAG ACCTTCAGCG GGATGGATCA GGTGCGGCTG
    ATGAAGAGGA TTGCAGAAAT GACAGATATC AAACCCATCC TGAGGAAGCT CCCGCGTATC
    AAGAAACACT TGTTAAATGG TGATAATATG AGGTGTTCAG TGAATGCGAC TCCTCAGCAG
    ATGCCTCAGA CAGAAAAAGC GGTCGAAGAC TTCCTTAGAA GCATCGGTCG GAGTAAAAAG
    GAACGGAGGC CTGTGCGCCC ACACACGGTC GAGAAACCTG TGCCCAGCAG CTCTGGTGGA
    GATGCCCACG TTCCCCATGG CTCCCAGGTC ATTAGGAAGC TGGTCATGGA ACCCACCTTC
    AAGCCCTGGC AGATGAAGAC TCACTTCCTG ATGCCCTTCC CGGTGAATTA CGTGGGTGAA
    TGCATCCGAA CTGTCCCCTA CACGGACCCA GATCATGCCA GTCTTAAAAT CCTTGCACGT
    TTGATGACTG CCAAATTCTT GCATACAGAA ATTCGAGAAA AAGGCGGTGC TTATGGTGGA
    GGCGCAAAAC TCAGCCACAA TGGGATTTTC ACCCTTTACT CTTACAGGGA CCCAAATACA
    ATAGAGACGC TCCAGTCTTT TGGGAAGGCT GTCGACTGGG CTAAGTCTGG AAAATTCACA
    CAGCAAGACA TCGACGAAGC CAAACTTTCT GTCTTCTCAA CCGTAGATGC TCCTGTCGCT
    CCTTCAGACA AAGGAATGGA CCACTTCTTG TACGGCCTCT CGGATGAGAT GAAGCAGGCC
    CACAGAGAGC AGCTCTTTGC TGTCAGCCAC GACAAGCTCC TGGCCGTGAG CGATAGATAC
    CTCGGCACTG GGAAGAGCAC ACACGGCCTG GCCATCCTCG GACCCGAGAA CCCGAAAATT
    GCCAAGGACC CATCCTGGAT CATCCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334658.1
    CDS504..3593
    Translation

    Target ORF information:

    RefSeq Version NM_001347729.1
    Organism Homo sapiens(human)
    Definition Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347729.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGTAATCC ACGAGCCCGT GTTTAATATA AGACATGCAC ACCACAGAGC GTGGCGATGG 
    AACAGTAACC GGGCTTGTGA GAGGGCTCTG CAGTATAAAC TAGGAGACAA GATCCATGGA
    TTCACCGTAA ACCAGGTGAC ATCTGTTCCC GAGCTGTTCC TGACTGCAGT GAAGCTCACC
    CATGATGACA CAGGAGCCAG GTATTTACAC CTGGCCAGAG AAGACACGAA TAATCTGTTC
    AGCGTGCAGT TCCGTACTAC TCCCATGGAC AGTACTGGTG TTCCTCACAT TCTTGAGCAT
    ACCGTCCTTT GTGGGTCTCA GAAATATCCG TGCAGAGACC CTTTCTTCAA AATGTTGAAC
    CGGTCCCTCT CCACGTTCAT GAACGCCTTC ACAGCTAGTG ATTATACTCT GTATCCATTT
    TCCACACAAA ATCCCAAGGA CTTTCAGAAT CTCCTCTCGG TGTATTTGGA TGCCACCTTT
    TTCCCATGTT TACGCGAGCT GGATTTCTGG CAGGAAGGAT GGCGGCTGGA ACATGAGAAT
    CCGAGCGACC CCCAGACGCC CTTGGTCTTT AAAGGAGTCG TCTTTAATGA GATGAAGGGA
    GCGTTTACAG ACAATGAGAG GATATTCTCC CAGCACCTTC AGAACAGACT TCTTCCTGAC
    CACACGTACT CAGTGGTCTC CGGGGGTGAC CCACTGTGCA TCCCGGAGCT TACATGGGAG
    CAGCTTAAGC AGTTTCATGC CACTCACTAT CACCCAAGCA ATGCTAGGTT CTTCACGTAC
    GGTAATTTTC CATTAGAACA GCATCTGAAA CAAATTCACG AGGAAGCACT GAGCAAATTC
    CAGAAAATTG AACCAAGCAC CGTGGTGCCA GCTCAGACAC CCTGGGACAA GCCTAGGGAA
    TTCCAGATAA CATGTGGCCC GGATTCATTT GCTACAGATC CCTCTAAACA AACAACCATC
    AGCGTTAGCT TCCTCTTACC GGACATCACC GACACATTTG AAGCCTTCAC ATTAAGTCTT
    CTGTCTTCAC TCTTGACTTC TGGGCCCAAT TCTCCCTTTT ACAAAGCCTT GATTGAATCT
    GGCCTTGGCA CAGACTTTTC TCCTGATGTT GGATATAATG GCTACACGAG GGAGGCCTAC
    TTTAGTGTCG GCCTCCAAGG GATTGCGGAG AAAGACATTG AGACCGTCAG AAGCCTCATA
    GACAGAACGA TTGATGAAGT AGTTGAGAAA GGATTTGAAG ATGATCGAAT TGAGGCTTTA
    CTTCATAAAA TTGAAATACA GATGAAACAT CAGTCTACCA GCTTTGGGCT GATGCTGACA
    TCATACATAG CTTCTTGCTG GAACCATGAT GGGGACCCTG TGGAGCTCTT GAAGTTGGGA
    AATCAGTTAG CTAAATTCAG ACAGTGCCTG CAGGAAAATC CAAAATTTTT GCAAGAAAAA
    GTAAAACAGT ATTTTAAGAA TAACCAGCAT AAGCTGACTT TATCGATGAG GCCAGATGAC
    AAGTATCACG AGAAGCAGGC ACAGGTGGAA GCCACGAAGC TCAAGCAGAA GGTCGAGGCT
    CTGTCCCCCG GAGACAGGCA GCAGATCTAC GAGAAAGGTC TAGAATTACG GAGTCAACAA
    AGCAAACCTC AAGATGCCTC TTGTCTGCCA GCGTTGAAAG TTTCCGATAT TGAACCCACC
    ATACCTGTCA CAGAGTTGGA CGTGGTCCTG ACAGCTGGAG ATATCCCTGT TCAGTACTGC
    GCCCAGCCCA CCAATGGCAT GGTGTATTTC CGGGCCTTCT CCAGCCTGAA CACACTCCCC
    GAGGAGCTGA GGCCCTATGT GCCCCTCTTC TGCAGCGTCC TCACCAAGCT GGGCTGCGGC
    CTTCTTGACT ACCGGGAGCA GGCTCAGCAG ATAGAATTGA AGACCGGAGG GATGAGTGCT
    TCTCCCCACG TGCTCCCCGA CGACTCACAC ATGGACACCT ACGAGCAGGG TGTGCTTTTC
    TCCTCTCTCT GCCTGGATCG AAACCTGCCA GACATGATGC AGCTATGGAG TGAAATATTT
    AACAACCCGT GCTTTGAAGA AGAGGAGCAC TTCAAGGTGC TGGTGAAGAT GACCGCCCAG
    GAGCTCGCCA ATGGAATTCC TGACTCTGGG CACCTGTACG CATCCATCAG GGCAGGCCGG
    ACCCTCACGC CCGCAGGGGA CCTGCAGGAG ACCTTCAGCG GGATGGATCA GGTGCGGCTG
    ATGAAGAGGA TTGCAGAAAT GACAGATATC AAACCCATCC TGAGGAAGCT CCCGCGTATC
    AAGAAACACT TGTTAAATGG TGATAATATG AGGTGTTCAG TGAATGCGAC TCCTCAGCAG
    ATGCCTCAGA CAGAAAAAGC GGTCGAAGAC TTCCTTAGAA GCATCGGTCG GAGTAAAAAG
    GAACGGAGGC CTGTGCGCCC ACACACGGTC GAGAAACCTG TGCCCAGCAG CTCTGGTGGA
    GATGCCCACG TTCCCCATGG CTCCCAGGTC ATTAGGAAGC TGGTCATGGA ACCCACCTTC
    AAGCCCTGGC AGATGAAGAC TCACTTCCTG ATGCCCTTCC CGGTGAATTA CGTGGGTGAA
    TGCATCCGAA CTGTCCCCTA CACGGACCCA GATCATGCCA GTCTTAAAAT CCTTGCACGT
    TTGATGACTG CCAAATTCTT GCATACAGAA ATTCGAGAAA AAGGCGGTGC TTATGGTGGA
    GGCGCAAAAC TCAGCCACAA TGGGATTTTC ACCCTTTACT CTTACAGGGA CCCAAATACA
    ATAGAGACGC TCCAGTCTTT TGGGAAGGCT GTCGACTGGG CTAAGTCTGG AAAATTCACA
    CAGCAAGACA TCGACGAAGC CAAACTTTCT GTCTTCTCAA CCGTAGATGC TCCTGTCGCT
    CCTTCAGACA AAGGAATGGA CCACTTCTTG TACGGCCTCT CGGATGAGAT GAAGCAGGCC
    CACAGAGAGC AGCTCTTTGC TGTCAGCCAC GACAAGCTCC TGGCCGTGAG CGATAGATAC
    CTCGGCACTG GGAAGAGCAC ACACGGCCTG GCCATCCTCG GACCCGAGAA CCCGAAAATT
    GCCAAGGACC CATCCTGGAT CATCCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu01770
    Clone ID Related Accession (Same CDS sequence) NM_001242307.1
    Accession Version NM_001242307.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3117bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-20
    Organism Homo sapiens(human)
    Product presequence protease, mitochondrial isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL451164.13, AF061243.1 and BC005025.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF061243.1, SRR1660809.137456.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTGGCGCT GCGGCGGGCG GCAGGGCCTG TGTGTGCTGA GGCGGCTGAG CGGCGGACAT 
    GCACACCACA GAGCGTGGCG ATGGAACAGT AACCGGGCTT GTGAGAGGGC TCTGCAGTAT
    AAACTAGGAG ACAAGATCCA TGGATTCACC GTAAACCAGG TGACATCTGT TCCCGAGCTG
    TTCCTGACTG CAGTGAAGCT CACCCATGAT GACACAGGAG CCAGGTATTT ACACCTGGCC
    AGAGAAGACA CGAATAATCT GTTCAGCGTG CAGTTCCGTA CTACTCCCAT GGACAGTACT
    GGTGTTCCTC ACATTCTTGA GCATACCGTC CTTTGTGGGT CTCAGAAATA TCCGTGCAGA
    GACCCTTTCT TCAAAATGTT GAACCGGTCC CTCTCCACGT TCATGAACGC CTTCACAGCT
    AGTGATTATA CTCTGTATCC ATTTTCCACA CAAAATCCCA AGGACTTTCA GAATCTCCTC
    TCGGTGTATT TGGATGCCAC CTTTTTCCCA TGTTTACGCG AGCTGGATTT CTGGCAGGAA
    GGATGGCGGC TGGAACATGA GAATCCGAGC GACCCCCAGA CGCCCTTGGT CTTTAAAGGA
    GTCGTCTTTA ATGAGATGAA GGGAGCGTTT ACAGACAATG AGAGGATATT CTCCCAGCAC
    CTTCAGAACA GACTTCTTCC TGACCACACG TACTCAGTGG TCTCCGGGGG TGACCCACTG
    TGCATCCCGG AGCTTACATG GGAGCAGCTT AAGCAGTTTC ATGCCACTCA CTATCACCCA
    AGCAATGCTA GGTTCTTCAC GTACGGTAAT TTTCCATTAG AACAGCATCT GAAACAAATT
    CACGAGGAAG CACTGAGCAA ATTCCAGAAA ATTGAACCAA GCACCGTGGT GCCAGCTCAG
    ACACCCTGGG ACAAGCCTAG GGAATTCCAG ATAACATGTG GCCCGGATTC ATTTGCTACA
    GATCCCTCTA AACAAACAAC CATCAGCGTT AGCTTCCTCT TACCGGACAT CACCGACACA
    TTTGAAGCCT TCACATTAAG TCTTCTGTCT TCACTCTTGA CTTCTGGGCC CAATTCTCCC
    TTTTACAAAG CCTTGATTGA ATCTGGCCTT GGCACAGACT TTTCTCCTGA TGTTGGATAT
    AATGGCTACA CGAGGGAGGC CTACTTTAGT GTCGGCCTCC AAGGGATTGC GGAGAAAGAC
    ATTGAGACCG TCAGAAGCCT CATAGACAGA ACGATTGATG AAGTAGTTGA GAAAGGATTT
    GAAGATGATC GAATTGAGGC TTTACTTCAT AAAATTGAAA TACAGATGAA ACATCAGTCT
    ACCAGCTTTG GGCTGATGCT GACATCATAC ATAGCTTCTT GCTGGAACCA TGATGGGGAC
    CCTGTGGAGC TCTTGAAGTT GGGAAATCAG TTAGCTAAAT TCAGACAGTG CCTGCAGGAA
    AATCCAAAAT TTTTGCAAGA AAAAGTAAAA CAGTATTTTA AGAATAACCA GCATAAGCTG
    ACTTTATCGA TGAGGCCAGA TGACAAGTAT CACGAGAAGC AGGCACAGGT GGAAGCCACG
    AAGCTCAAGC AGAAGGTCGA GGCTCTGTCC CCCGGAGACA GGCAGCAGAT CTACGAGAAA
    GGTCTAGAAT TACGGAGTCA ACAAAGCAAA CCTCAAGATG CCTCTTGTCT GCCAGCGTTG
    AAAGTTTCCG ATATTGAACC CACCATACCT GTCACAGAGT TGGACGTGGT CCTGACAGCT
    GGAGATATCC CTGTTCAGTA CTGCGCCCAG CCCACCAATG GCATGGTGTA TTTCCGGGCC
    TTCTCCAGCC TGAACACACT CCCCGAGGAG CTGAGGCCCT ATGTGCCCCT CTTCTGCAGC
    GTCCTCACCA AGCTGGGCTG CGGCCTTCTT GACTACCGGG AGCAGGCTCA GCAGATAGAA
    TTGAAGACCG GAGGGATGAG TGCTTCTCCC CACGTGCTCC CCGACGACTC ACACATGGAC
    ACCTACGAGC AGGTAGGTGT GCTTTTCTCC TCTCTCTGCC TGGATCGAAA CCTGCCAGAC
    ATGATGCAGC TATGGAGTGA AATATTTAAC AACCCGTGCT TTGAAGAAGA GGAGCACTTC
    AAGGTGCTGG TGAAGATGAC CGCCCAGGAG CTCGCCAATG GAATTCCTGA CTCTGGGCAC
    CTGTACGCAT CCATCAGGGC AGGCCGGACC CTCACGCCCG CAGGGGACCT GCAGGAGACC
    TTCAGCGGGA TGGATCAGGT GCGGCTGATG AAGAGGATTG CAGAAATGAC AGATATCAAA
    CCCATCCTGA GGAAGCTCCC GCGTATCAAG AAACACTTGT TAAATGGTGA TAATATGAGG
    TGTTCAGTGA ATGCGACTCC TCAGCAGATG CCTCAGACAG AAAAAGCGGT CGAAGACTTC
    CTTAGAAGCA TCGGTCGGAG TAAAAAGGAA CGGAGGCCTG TGCGCCCACA CACGGTCGAG
    AAACCTGTGC CCAGCAGCTC TGGTGGAGAT GCCCACGTTC CCCATGGCTC CCAGGTCATT
    AGGAAGCTGG TCATGGAACC CACCTTCAAG CCCTGGCAGA TGAAGACTCA CTTCCTGATG
    CCCTTCCCGG TGAATTACGT GGGTGAATGC ATCCGAACTG TCCCCTACAC GGACCCAGAT
    CATGCCAGTC TTAAAATCCT TGCACGTTTG ATGACTGCCA AATTCTTGCA TACAGAAATT
    CGAGAAAAAG GCGGTGCTTA TGGTGGAGGC GCAAAACTCA GCCACAATGG GATTTTCACC
    CTTTACTCTT ACAGGGACCC AAATACAATA GAGACGCTCC AGTCTTTTGG GAAGGCTGTC
    GACTGGGCTA AGTCTGGAAA ATTCACACAG CAAGACATCG ACGAAGCCAA ACTTTCTGTC
    TTCTCAACCG TAGATGCTCC TGTCGCTCCT TCAGACAAAG GAATGGACCA CTTCTTGTAC
    GGCCTCTCGG ATGAGATGAA GCAGGCCCAC AGAGAGCAGC TCTTTGCTGT CAGCCACGAC
    AAGCTCCTGG CCGTGAGCGA TAGATACCTC GGCACTGGGA AGAGCACACA CGGCCTGGCC
    ATCCTCGGAC CCGAGAACCC GAAAATTGCC AAGGACCCAT CCTGGATCAT CCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001229236.1
    CDS70..3186
    Translation

    Target ORF information:

    RefSeq Version NM_001242307.1
    Organism Homo sapiens(human)
    Definition Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001242307.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTGGCGCT GCGGCGGGCG GCAGGGCCTG TGTGTGCTGA GGCGGCTGAG CGGCGGACAT 
    GCACACCACA GAGCGTGGCG ATGGAACAGT AACCGGGCTT GTGAGAGGGC TCTGCAGTAT
    AAACTAGGAG ACAAGATCCA TGGATTCACC GTAAACCAGG TGACATCTGT TCCCGAGCTG
    TTCCTGACTG CAGTGAAGCT CACCCATGAT GACACAGGAG CCAGGTATTT ACACCTGGCC
    AGAGAAGACA CGAATAATCT GTTCAGCGTG CAGTTCCGTA CTACTCCCAT GGACAGTACT
    GGTGTTCCTC ACATTCTTGA GCATACCGTC CTTTGTGGGT CTCAGAAATA TCCGTGCAGA
    GACCCTTTCT TCAAAATGTT GAACCGGTCC CTCTCCACGT TCATGAACGC CTTCACAGCT
    AGTGATTATA CTCTGTATCC ATTTTCCACA CAAAATCCCA AGGACTTTCA GAATCTCCTC
    TCGGTGTATT TGGATGCCAC CTTTTTCCCA TGTTTACGCG AGCTGGATTT CTGGCAGGAA
    GGATGGCGGC TGGAACATGA GAATCCGAGC GACCCCCAGA CGCCCTTGGT CTTTAAAGGA
    GTCGTCTTTA ATGAGATGAA GGGAGCGTTT ACAGACAATG AGAGGATATT CTCCCAGCAC
    CTTCAGAACA GACTTCTTCC TGACCACACG TACTCAGTGG TCTCCGGGGG TGACCCACTG
    TGCATCCCGG AGCTTACATG GGAGCAGCTT AAGCAGTTTC ATGCCACTCA CTATCACCCA
    AGCAATGCTA GGTTCTTCAC GTACGGTAAT TTTCCATTAG AACAGCATCT GAAACAAATT
    CACGAGGAAG CACTGAGCAA ATTCCAGAAA ATTGAACCAA GCACCGTGGT GCCAGCTCAG
    ACACCCTGGG ACAAGCCTAG GGAATTCCAG ATAACATGTG GCCCGGATTC ATTTGCTACA
    GATCCCTCTA AACAAACAAC CATCAGCGTT AGCTTCCTCT TACCGGACAT CACCGACACA
    TTTGAAGCCT TCACATTAAG TCTTCTGTCT TCACTCTTGA CTTCTGGGCC CAATTCTCCC
    TTTTACAAAG CCTTGATTGA ATCTGGCCTT GGCACAGACT TTTCTCCTGA TGTTGGATAT
    AATGGCTACA CGAGGGAGGC CTACTTTAGT GTCGGCCTCC AAGGGATTGC GGAGAAAGAC
    ATTGAGACCG TCAGAAGCCT CATAGACAGA ACGATTGATG AAGTAGTTGA GAAAGGATTT
    GAAGATGATC GAATTGAGGC TTTACTTCAT AAAATTGAAA TACAGATGAA ACATCAGTCT
    ACCAGCTTTG GGCTGATGCT GACATCATAC ATAGCTTCTT GCTGGAACCA TGATGGGGAC
    CCTGTGGAGC TCTTGAAGTT GGGAAATCAG TTAGCTAAAT TCAGACAGTG CCTGCAGGAA
    AATCCAAAAT TTTTGCAAGA AAAAGTAAAA CAGTATTTTA AGAATAACCA GCATAAGCTG
    ACTTTATCGA TGAGGCCAGA TGACAAGTAT CACGAGAAGC AGGCACAGGT GGAAGCCACG
    AAGCTCAAGC AGAAGGTCGA GGCTCTGTCC CCCGGAGACA GGCAGCAGAT CTACGAGAAA
    GGTCTAGAAT TACGGAGTCA ACAAAGCAAA CCTCAAGATG CCTCTTGTCT GCCAGCGTTG
    AAAGTTTCCG ATATTGAACC CACCATACCT GTCACAGAGT TGGACGTGGT CCTGACAGCT
    GGAGATATCC CTGTTCAGTA CTGCGCCCAG CCCACCAATG GCATGGTGTA TTTCCGGGCC
    TTCTCCAGCC TGAACACACT CCCCGAGGAG CTGAGGCCCT ATGTGCCCCT CTTCTGCAGC
    GTCCTCACCA AGCTGGGCTG CGGCCTTCTT GACTACCGGG AGCAGGCTCA GCAGATAGAA
    TTGAAGACCG GAGGGATGAG TGCTTCTCCC CACGTGCTCC CCGACGACTC ACACATGGAC
    ACCTACGAGC AGGTAGGTGT GCTTTTCTCC TCTCTCTGCC TGGATCGAAA CCTGCCAGAC
    ATGATGCAGC TATGGAGTGA AATATTTAAC AACCCGTGCT TTGAAGAAGA GGAGCACTTC
    AAGGTGCTGG TGAAGATGAC CGCCCAGGAG CTCGCCAATG GAATTCCTGA CTCTGGGCAC
    CTGTACGCAT CCATCAGGGC AGGCCGGACC CTCACGCCCG CAGGGGACCT GCAGGAGACC
    TTCAGCGGGA TGGATCAGGT GCGGCTGATG AAGAGGATTG CAGAAATGAC AGATATCAAA
    CCCATCCTGA GGAAGCTCCC GCGTATCAAG AAACACTTGT TAAATGGTGA TAATATGAGG
    TGTTCAGTGA ATGCGACTCC TCAGCAGATG CCTCAGACAG AAAAAGCGGT CGAAGACTTC
    CTTAGAAGCA TCGGTCGGAG TAAAAAGGAA CGGAGGCCTG TGCGCCCACA CACGGTCGAG
    AAACCTGTGC CCAGCAGCTC TGGTGGAGAT GCCCACGTTC CCCATGGCTC CCAGGTCATT
    AGGAAGCTGG TCATGGAACC CACCTTCAAG CCCTGGCAGA TGAAGACTCA CTTCCTGATG
    CCCTTCCCGG TGAATTACGT GGGTGAATGC ATCCGAACTG TCCCCTACAC GGACCCAGAT
    CATGCCAGTC TTAAAATCCT TGCACGTTTG ATGACTGCCA AATTCTTGCA TACAGAAATT
    CGAGAAAAAG GCGGTGCTTA TGGTGGAGGC GCAAAACTCA GCCACAATGG GATTTTCACC
    CTTTACTCTT ACAGGGACCC AAATACAATA GAGACGCTCC AGTCTTTTGG GAAGGCTGTC
    GACTGGGCTA AGTCTGGAAA ATTCACACAG CAAGACATCG ACGAAGCCAA ACTTTCTGTC
    TTCTCAACCG TAGATGCTCC TGTCGCTCCT TCAGACAAAG GAATGGACCA CTTCTTGTAC
    GGCCTCTCGG ATGAGATGAA GCAGGCCCAC AGAGAGCAGC TCTTTGCTGT CAGCCACGAC
    AAGCTCCTGG CCGTGAGCGA TAGATACCTC GGCACTGGGA AGAGCACACA CGGCCTGGCC
    ATCCTCGGAC CCGAGAACCC GAAAATTGCC AAGGACCCAT CCTGGATCAT CCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu01814
    Clone ID Related Accession (Same CDS sequence) NM_014889.3 , NM_014889.4
    Accession Version NM_014889.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3114bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Homo sapiens(human)
    Product presequence protease, mitochondrial isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL451164.13, BC113369.1 and BC005025.1. On May 26, 2011 this sequence version replaced NM_014889.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC005025.1, SRR1803615.3112.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTGGCGCT GCGGCGGGCG GCAGGGCCTG TGTGTGCTGA GGCGGCTGAG CGGCGGACAT 
    GCACACCACA GAGCGTGGCG ATGGAACAGT AACCGGGCTT GTGAGAGGGC TCTGCAGTAT
    AAACTAGGAG ACAAGATCCA TGGATTCACC GTAAACCAGG TGACATCTGT TCCCGAGCTG
    TTCCTGACTG CAGTGAAGCT CACCCATGAT GACACAGGAG CCAGGTATTT ACACCTGGCC
    AGAGAAGACA CGAATAATCT GTTCAGCGTG CAGTTCCGTA CTACTCCCAT GGACAGTACT
    GGTGTTCCTC ACATTCTTGA GCATACCGTC CTTTGTGGGT CTCAGAAATA TCCGTGCAGA
    GACCCTTTCT TCAAAATGTT GAACCGGTCC CTCTCCACGT TCATGAACGC CTTCACAGCT
    AGTGATTATA CTCTGTATCC ATTTTCCACA CAAAATCCCA AGGACTTTCA GAATCTCCTC
    TCGGTGTATT TGGATGCCAC CTTTTTCCCA TGTTTACGCG AGCTGGATTT CTGGCAGGAA
    GGATGGCGGC TGGAACATGA GAATCCGAGC GACCCCCAGA CGCCCTTGGT CTTTAAAGGA
    GTCGTCTTTA ATGAGATGAA GGGAGCGTTT ACAGACAATG AGAGGATATT CTCCCAGCAC
    CTTCAGAACA GACTTCTTCC TGACCACACG TACTCAGTGG TCTCCGGGGG TGACCCACTG
    TGCATCCCGG AGCTTACATG GGAGCAGCTT AAGCAGTTTC ATGCCACTCA CTATCACCCA
    AGCAATGCTA GGTTCTTCAC GTACGGTAAT TTTCCATTAG AACAGCATCT GAAACAAATT
    CACGAGGAAG CACTGAGCAA ATTCCAGAAA ATTGAACCAA GCACCGTGGT GCCAGCTCAG
    ACACCCTGGG ACAAGCCTAG GGAATTCCAG ATAACATGTG GCCCGGATTC ATTTGCTACA
    GATCCCTCTA AACAAACAAC CATCAGCGTT AGCTTCCTCT TACCGGACAT CACCGACACA
    TTTGAAGCCT TCACATTAAG TCTTCTGTCT TCACTCTTGA CTTCTGGGCC CAATTCTCCC
    TTTTACAAAG CCTTGATTGA ATCTGGCCTT GGCACAGACT TTTCTCCTGA TGTTGGATAT
    AATGGCTACA CGAGGGAGGC CTACTTTAGT GTCGGCCTCC AAGGGATTGC GGAGAAAGAC
    ATTGAGACCG TCAGAAGCCT CATAGACAGA ACGATTGATG AAGTAGTTGA GAAAGGATTT
    GAAGATGATC GAATTGAGGC TTTACTTCAT AAAATTGAAA TACAGATGAA ACATCAGTCT
    ACCAGCTTTG GGCTGATGCT GACATCATAC ATAGCTTCTT GCTGGAACCA TGATGGGGAC
    CCTGTGGAGC TCTTGAAGTT GGGAAATCAG TTAGCTAAAT TCAGACAGTG CCTGCAGGAA
    AATCCAAAAT TTTTGCAAGA AAAAGTAAAA CAGTATTTTA AGAATAACCA GCATAAGCTG
    ACTTTATCGA TGAGGCCAGA TGACAAGTAT CACGAGAAGC AGGCACAGGT GGAAGCCACG
    AAGCTCAAGC AGAAGGTCGA GGCTCTGTCC CCCGGAGACA GGCAGCAGAT CTACGAGAAA
    GGTCTAGAAT TACGGAGTCA ACAAAGCAAA CCTCAAGATG CCTCTTGTCT GCCAGCGTTG
    AAAGTTTCCG ATATTGAACC CACCATACCT GTCACAGAGT TGGACGTGGT CCTGACAGCT
    GGAGATATCC CTGTTCAGTA CTGCGCCCAG CCCACCAATG GCATGGTGTA TTTCCGGGCC
    TTCTCCAGCC TGAACACACT CCCCGAGGAG CTGAGGCCCT ATGTGCCCCT CTTCTGCAGC
    GTCCTCACCA AGCTGGGCTG CGGCCTTCTT GACTACCGGG AGCAGGCTCA GCAGATAGAA
    TTGAAGACCG GAGGGATGAG TGCTTCTCCC CACGTGCTCC CCGACGACTC ACACATGGAC
    ACCTACGAGC AGGGTGTGCT TTTCTCCTCT CTCTGCCTGG ATCGAAACCT GCCAGACATG
    ATGCAGCTAT GGAGTGAAAT ATTTAACAAC CCGTGCTTTG AAGAAGAGGA GCACTTCAAG
    GTGCTGGTGA AGATGACCGC CCAGGAGCTC GCCAATGGAA TTCCTGACTC TGGGCACCTG
    TACGCATCCA TCAGGGCAGG CCGGACCCTC ACGCCCGCAG GGGACCTGCA GGAGACCTTC
    AGCGGGATGG ATCAGGTGCG GCTGATGAAG AGGATTGCAG AAATGACAGA TATCAAACCC
    ATCCTGAGGA AGCTCCCGCG TATCAAGAAA CACTTGTTAA ATGGTGATAA TATGAGGTGT
    TCAGTGAATG CGACTCCTCA GCAGATGCCT CAGACAGAAA AAGCGGTCGA AGACTTCCTT
    AGAAGCATCG GTCGGAGTAA AAAGGAACGG AGGCCTGTGC GCCCACACAC GGTCGAGAAA
    CCTGTGCCCA GCAGCTCTGG TGGAGATGCC CACGTTCCCC ATGGCTCCCA GGTCATTAGG
    AAGCTGGTCA TGGAACCCAC CTTCAAGCCC TGGCAGATGA AGACTCACTT CCTGATGCCC
    TTCCCGGTGA ATTACGTGGG TGAATGCATC CGAACTGTCC CCTACACGGA CCCAGATCAT
    GCCAGTCTTA AAATCCTTGC ACGTTTGATG ACTGCCAAAT TCTTGCATAC AGAAATTCGA
    GAAAAAGGCG GTGCTTATGG TGGAGGCGCA AAACTCAGCC ACAATGGGAT TTTCACCCTT
    TACTCTTACA GGGACCCAAA TACAATAGAG ACGCTCCAGT CTTTTGGGAA GGCTGTCGAC
    TGGGCTAAGT CTGGAAAATT CACACAGCAA GACATCGACG AAGCCAAACT TTCTGTCTTC
    TCAACCGTAG ATGCTCCTGT CGCTCCTTCA GACAAAGGAA TGGACCACTT CTTGTACGGC
    CTCTCGGATG AGATGAAGCA GGCCCACAGA GAGCAGCTCT TTGCTGTCAG CCACGACAAG
    CTCCTGGCCG TGAGCGATAG ATACCTCGGC ACTGGGAAGA GCACACACGG CCTGGCCATC
    CTCGGACCCG AGAACCCGAA AATTGCCAAG GACCCATCCT GGATCATCCA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_055704.2
    CDS70..3183
    Translation

    Target ORF information:

    RefSeq Version NM_014889.3
    Organism Homo sapiens(human)
    Definition Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_014889.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTGGCGCT GCGGCGGGCG GCAGGGCCTG TGTGTGCTGA GGCGGCTGAG CGGCGGACAT 
    GCACACCACA GAGCGTGGCG ATGGAACAGT AACCGGGCTT GTGAGAGGGC TCTGCAGTAT
    AAACTAGGAG ACAAGATCCA TGGATTCACC GTAAACCAGG TGACATCTGT TCCCGAGCTG
    TTCCTGACTG CAGTGAAGCT CACCCATGAT GACACAGGAG CCAGGTATTT ACACCTGGCC
    AGAGAAGACA CGAATAATCT GTTCAGCGTG CAGTTCCGTA CTACTCCCAT GGACAGTACT
    GGTGTTCCTC ACATTCTTGA GCATACCGTC CTTTGTGGGT CTCAGAAATA TCCGTGCAGA
    GACCCTTTCT TCAAAATGTT GAACCGGTCC CTCTCCACGT TCATGAACGC CTTCACAGCT
    AGTGATTATA CTCTGTATCC ATTTTCCACA CAAAATCCCA AGGACTTTCA GAATCTCCTC
    TCGGTGTATT TGGATGCCAC CTTTTTCCCA TGTTTACGCG AGCTGGATTT CTGGCAGGAA
    GGATGGCGGC TGGAACATGA GAATCCGAGC GACCCCCAGA CGCCCTTGGT CTTTAAAGGA
    GTCGTCTTTA ATGAGATGAA GGGAGCGTTT ACAGACAATG AGAGGATATT CTCCCAGCAC
    CTTCAGAACA GACTTCTTCC TGACCACACG TACTCAGTGG TCTCCGGGGG TGACCCACTG
    TGCATCCCGG AGCTTACATG GGAGCAGCTT AAGCAGTTTC ATGCCACTCA CTATCACCCA
    AGCAATGCTA GGTTCTTCAC GTACGGTAAT TTTCCATTAG AACAGCATCT GAAACAAATT
    CACGAGGAAG CACTGAGCAA ATTCCAGAAA ATTGAACCAA GCACCGTGGT GCCAGCTCAG
    ACACCCTGGG ACAAGCCTAG GGAATTCCAG ATAACATGTG GCCCGGATTC ATTTGCTACA
    GATCCCTCTA AACAAACAAC CATCAGCGTT AGCTTCCTCT TACCGGACAT CACCGACACA
    TTTGAAGCCT TCACATTAAG TCTTCTGTCT TCACTCTTGA CTTCTGGGCC CAATTCTCCC
    TTTTACAAAG CCTTGATTGA ATCTGGCCTT GGCACAGACT TTTCTCCTGA TGTTGGATAT
    AATGGCTACA CGAGGGAGGC CTACTTTAGT GTCGGCCTCC AAGGGATTGC GGAGAAAGAC
    ATTGAGACCG TCAGAAGCCT CATAGACAGA ACGATTGATG AAGTAGTTGA GAAAGGATTT
    GAAGATGATC GAATTGAGGC TTTACTTCAT AAAATTGAAA TACAGATGAA ACATCAGTCT
    ACCAGCTTTG GGCTGATGCT GACATCATAC ATAGCTTCTT GCTGGAACCA TGATGGGGAC
    CCTGTGGAGC TCTTGAAGTT GGGAAATCAG TTAGCTAAAT TCAGACAGTG CCTGCAGGAA
    AATCCAAAAT TTTTGCAAGA AAAAGTAAAA CAGTATTTTA AGAATAACCA GCATAAGCTG
    ACTTTATCGA TGAGGCCAGA TGACAAGTAT CACGAGAAGC AGGCACAGGT GGAAGCCACG
    AAGCTCAAGC AGAAGGTCGA GGCTCTGTCC CCCGGAGACA GGCAGCAGAT CTACGAGAAA
    GGTCTAGAAT TACGGAGTCA ACAAAGCAAA CCTCAAGATG CCTCTTGTCT GCCAGCGTTG
    AAAGTTTCCG ATATTGAACC CACCATACCT GTCACAGAGT TGGACGTGGT CCTGACAGCT
    GGAGATATCC CTGTTCAGTA CTGCGCCCAG CCCACCAATG GCATGGTGTA TTTCCGGGCC
    TTCTCCAGCC TGAACACACT CCCCGAGGAG CTGAGGCCCT ATGTGCCCCT CTTCTGCAGC
    GTCCTCACCA AGCTGGGCTG CGGCCTTCTT GACTACCGGG AGCAGGCTCA GCAGATAGAA
    TTGAAGACCG GAGGGATGAG TGCTTCTCCC CACGTGCTCC CCGACGACTC ACACATGGAC
    ACCTACGAGC AGGGTGTGCT TTTCTCCTCT CTCTGCCTGG ATCGAAACCT GCCAGACATG
    ATGCAGCTAT GGAGTGAAAT ATTTAACAAC CCGTGCTTTG AAGAAGAGGA GCACTTCAAG
    GTGCTGGTGA AGATGACCGC CCAGGAGCTC GCCAATGGAA TTCCTGACTC TGGGCACCTG
    TACGCATCCA TCAGGGCAGG CCGGACCCTC ACGCCCGCAG GGGACCTGCA GGAGACCTTC
    AGCGGGATGG ATCAGGTGCG GCTGATGAAG AGGATTGCAG AAATGACAGA TATCAAACCC
    ATCCTGAGGA AGCTCCCGCG TATCAAGAAA CACTTGTTAA ATGGTGATAA TATGAGGTGT
    TCAGTGAATG CGACTCCTCA GCAGATGCCT CAGACAGAAA AAGCGGTCGA AGACTTCCTT
    AGAAGCATCG GTCGGAGTAA AAAGGAACGG AGGCCTGTGC GCCCACACAC GGTCGAGAAA
    CCTGTGCCCA GCAGCTCTGG TGGAGATGCC CACGTTCCCC ATGGCTCCCA GGTCATTAGG
    AAGCTGGTCA TGGAACCCAC CTTCAAGCCC TGGCAGATGA AGACTCACTT CCTGATGCCC
    TTCCCGGTGA ATTACGTGGG TGAATGCATC CGAACTGTCC CCTACACGGA CCCAGATCAT
    GCCAGTCTTA AAATCCTTGC ACGTTTGATG ACTGCCAAAT TCTTGCATAC AGAAATTCGA
    GAAAAAGGCG GTGCTTATGG TGGAGGCGCA AAACTCAGCC ACAATGGGAT TTTCACCCTT
    TACTCTTACA GGGACCCAAA TACAATAGAG ACGCTCCAGT CTTTTGGGAA GGCTGTCGAC
    TGGGCTAAGT CTGGAAAATT CACACAGCAA GACATCGACG AAGCCAAACT TTCTGTCTTC
    TCAACCGTAG ATGCTCCTGT CGCTCCTTCA GACAAAGGAA TGGACCACTT CTTGTACGGC
    CTCTCGGATG AGATGAAGCA GGCCCACAGA GAGCAGCTCT TTGCTGTCAG CCACGACAAG
    CTCCTGGCCG TGAGCGATAG ATACCTCGGC ACTGGGAAGA GCACACACGG CCTGGCCATC
    CTCGGACCCG AGAACCCGAA AATTGCCAAG GACCCATCCT GGATCATCCA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu01814
    Clone ID Related Accession (Same CDS sequence) NM_014889.3 , NM_014889.4
    Accession Version NM_014889.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3114bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-23
    Organism Homo sapiens(human)
    Product presequence protease, mitochondrial isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC113369.1, AL451164.13 and BC005025.1. On Nov 23, 2018 this sequence version replaced NM_014889.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC095422.1, SRR1660803.139369.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000224949.9/ ENSP00000224949.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTGGCGCT GCGGCGGGCG GCAGGGCCTG TGTGTGCTGA GGCGGCTGAG CGGCGGACAT 
    GCACACCACA GAGCGTGGCG ATGGAACAGT AACCGGGCTT GTGAGAGGGC TCTGCAGTAT
    AAACTAGGAG ACAAGATCCA TGGATTCACC GTAAACCAGG TGACATCTGT TCCCGAGCTG
    TTCCTGACTG CAGTGAAGCT CACCCATGAT GACACAGGAG CCAGGTATTT ACACCTGGCC
    AGAGAAGACA CGAATAATCT GTTCAGCGTG CAGTTCCGTA CTACTCCCAT GGACAGTACT
    GGTGTTCCTC ACATTCTTGA GCATACCGTC CTTTGTGGGT CTCAGAAATA TCCGTGCAGA
    GACCCTTTCT TCAAAATGTT GAACCGGTCC CTCTCCACGT TCATGAACGC CTTCACAGCT
    AGTGATTATA CTCTGTATCC ATTTTCCACA CAAAATCCCA AGGACTTTCA GAATCTCCTC
    TCGGTGTATT TGGATGCCAC CTTTTTCCCA TGTTTACGCG AGCTGGATTT CTGGCAGGAA
    GGATGGCGGC TGGAACATGA GAATCCGAGC GACCCCCAGA CGCCCTTGGT CTTTAAAGGA
    GTCGTCTTTA ATGAGATGAA GGGAGCGTTT ACAGACAATG AGAGGATATT CTCCCAGCAC
    CTTCAGAACA GACTTCTTCC TGACCACACG TACTCAGTGG TCTCCGGGGG TGACCCACTG
    TGCATCCCGG AGCTTACATG GGAGCAGCTT AAGCAGTTTC ATGCCACTCA CTATCACCCA
    AGCAATGCTA GGTTCTTCAC GTACGGTAAT TTTCCATTAG AACAGCATCT GAAACAAATT
    CACGAGGAAG CACTGAGCAA ATTCCAGAAA ATTGAACCAA GCACCGTGGT GCCAGCTCAG
    ACACCCTGGG ACAAGCCTAG GGAATTCCAG ATAACATGTG GCCCGGATTC ATTTGCTACA
    GATCCCTCTA AACAAACAAC CATCAGCGTT AGCTTCCTCT TACCGGACAT CACCGACACA
    TTTGAAGCCT TCACATTAAG TCTTCTGTCT TCACTCTTGA CTTCTGGGCC CAATTCTCCC
    TTTTACAAAG CCTTGATTGA ATCTGGCCTT GGCACAGACT TTTCTCCTGA TGTTGGATAT
    AATGGCTACA CGAGGGAGGC CTACTTTAGT GTCGGCCTCC AAGGGATTGC GGAGAAAGAC
    ATTGAGACCG TCAGAAGCCT CATAGACAGA ACGATTGATG AAGTAGTTGA GAAAGGATTT
    GAAGATGATC GAATTGAGGC TTTACTTCAT AAAATTGAAA TACAGATGAA ACATCAGTCT
    ACCAGCTTTG GGCTGATGCT GACATCATAC ATAGCTTCTT GCTGGAACCA TGATGGGGAC
    CCTGTGGAGC TCTTGAAGTT GGGAAATCAG TTAGCTAAAT TCAGACAGTG CCTGCAGGAA
    AATCCAAAAT TTTTGCAAGA AAAAGTAAAA CAGTATTTTA AGAATAACCA GCATAAGCTG
    ACTTTATCGA TGAGGCCAGA TGACAAGTAT CACGAGAAGC AGGCACAGGT GGAAGCCACG
    AAGCTCAAGC AGAAGGTCGA GGCTCTGTCC CCCGGAGACA GGCAGCAGAT CTACGAGAAA
    GGTCTAGAAT TACGGAGTCA ACAAAGCAAA CCTCAAGATG CCTCTTGTCT GCCAGCGTTG
    AAAGTTTCCG ATATTGAACC CACCATACCT GTCACAGAGT TGGACGTGGT CCTGACAGCT
    GGAGATATCC CTGTTCAGTA CTGCGCCCAG CCCACCAATG GCATGGTGTA TTTCCGGGCC
    TTCTCCAGCC TGAACACACT CCCCGAGGAG CTGAGGCCCT ATGTGCCCCT CTTCTGCAGC
    GTCCTCACCA AGCTGGGCTG CGGCCTTCTT GACTACCGGG AGCAGGCTCA GCAGATAGAA
    TTGAAGACCG GAGGGATGAG TGCTTCTCCC CACGTGCTCC CCGACGACTC ACACATGGAC
    ACCTACGAGC AGGGTGTGCT TTTCTCCTCT CTCTGCCTGG ATCGAAACCT GCCAGACATG
    ATGCAGCTAT GGAGTGAAAT ATTTAACAAC CCGTGCTTTG AAGAAGAGGA GCACTTCAAG
    GTGCTGGTGA AGATGACCGC CCAGGAGCTC GCCAATGGAA TTCCTGACTC TGGGCACCTG
    TACGCATCCA TCAGGGCAGG CCGGACCCTC ACGCCCGCAG GGGACCTGCA GGAGACCTTC
    AGCGGGATGG ATCAGGTGCG GCTGATGAAG AGGATTGCAG AAATGACAGA TATCAAACCC
    ATCCTGAGGA AGCTCCCGCG TATCAAGAAA CACTTGTTAA ATGGTGATAA TATGAGGTGT
    TCAGTGAATG CGACTCCTCA GCAGATGCCT CAGACAGAAA AAGCGGTCGA AGACTTCCTT
    AGAAGCATCG GTCGGAGTAA AAAGGAACGG AGGCCTGTGC GCCCACACAC GGTCGAGAAA
    CCTGTGCCCA GCAGCTCTGG TGGAGATGCC CACGTTCCCC ATGGCTCCCA GGTCATTAGG
    AAGCTGGTCA TGGAACCCAC CTTCAAGCCC TGGCAGATGA AGACTCACTT CCTGATGCCC
    TTCCCGGTGA ATTACGTGGG TGAATGCATC CGAACTGTCC CCTACACGGA CCCAGATCAT
    GCCAGTCTTA AAATCCTTGC ACGTTTGATG ACTGCCAAAT TCTTGCATAC AGAAATTCGA
    GAAAAAGGCG GTGCTTATGG TGGAGGCGCA AAACTCAGCC ACAATGGGAT TTTCACCCTT
    TACTCTTACA GGGACCCAAA TACAATAGAG ACGCTCCAGT CTTTTGGGAA GGCTGTCGAC
    TGGGCTAAGT CTGGAAAATT CACACAGCAA GACATCGACG AAGCCAAACT TTCTGTCTTC
    TCAACCGTAG ATGCTCCTGT CGCTCCTTCA GACAAAGGAA TGGACCACTT CTTGTACGGC
    CTCTCGGATG AGATGAAGCA GGCCCACAGA GAGCAGCTCT TTGCTGTCAG CCACGACAAG
    CTCCTGGCCG TGAGCGATAG ATACCTCGGC ACTGGGAAGA GCACACACGG CCTGGCCATC
    CTCGGACCCG AGAACCCGAA AATTGCCAAG GACCCATCCT GGATCATCCA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_055704.2
    CDS11..3124
    Translation

    Target ORF information:

    RefSeq Version NM_014889.4
    Organism Homo sapiens(human)
    Definition Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_014889.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTGGCGCT GCGGCGGGCG GCAGGGCCTG TGTGTGCTGA GGCGGCTGAG CGGCGGACAT 
    GCACACCACA GAGCGTGGCG ATGGAACAGT AACCGGGCTT GTGAGAGGGC TCTGCAGTAT
    AAACTAGGAG ACAAGATCCA TGGATTCACC GTAAACCAGG TGACATCTGT TCCCGAGCTG
    TTCCTGACTG CAGTGAAGCT CACCCATGAT GACACAGGAG CCAGGTATTT ACACCTGGCC
    AGAGAAGACA CGAATAATCT GTTCAGCGTG CAGTTCCGTA CTACTCCCAT GGACAGTACT
    GGTGTTCCTC ACATTCTTGA GCATACCGTC CTTTGTGGGT CTCAGAAATA TCCGTGCAGA
    GACCCTTTCT TCAAAATGTT GAACCGGTCC CTCTCCACGT TCATGAACGC CTTCACAGCT
    AGTGATTATA CTCTGTATCC ATTTTCCACA CAAAATCCCA AGGACTTTCA GAATCTCCTC
    TCGGTGTATT TGGATGCCAC CTTTTTCCCA TGTTTACGCG AGCTGGATTT CTGGCAGGAA
    GGATGGCGGC TGGAACATGA GAATCCGAGC GACCCCCAGA CGCCCTTGGT CTTTAAAGGA
    GTCGTCTTTA ATGAGATGAA GGGAGCGTTT ACAGACAATG AGAGGATATT CTCCCAGCAC
    CTTCAGAACA GACTTCTTCC TGACCACACG TACTCAGTGG TCTCCGGGGG TGACCCACTG
    TGCATCCCGG AGCTTACATG GGAGCAGCTT AAGCAGTTTC ATGCCACTCA CTATCACCCA
    AGCAATGCTA GGTTCTTCAC GTACGGTAAT TTTCCATTAG AACAGCATCT GAAACAAATT
    CACGAGGAAG CACTGAGCAA ATTCCAGAAA ATTGAACCAA GCACCGTGGT GCCAGCTCAG
    ACACCCTGGG ACAAGCCTAG GGAATTCCAG ATAACATGTG GCCCGGATTC ATTTGCTACA
    GATCCCTCTA AACAAACAAC CATCAGCGTT AGCTTCCTCT TACCGGACAT CACCGACACA
    TTTGAAGCCT TCACATTAAG TCTTCTGTCT TCACTCTTGA CTTCTGGGCC CAATTCTCCC
    TTTTACAAAG CCTTGATTGA ATCTGGCCTT GGCACAGACT TTTCTCCTGA TGTTGGATAT
    AATGGCTACA CGAGGGAGGC CTACTTTAGT GTCGGCCTCC AAGGGATTGC GGAGAAAGAC
    ATTGAGACCG TCAGAAGCCT CATAGACAGA ACGATTGATG AAGTAGTTGA GAAAGGATTT
    GAAGATGATC GAATTGAGGC TTTACTTCAT AAAATTGAAA TACAGATGAA ACATCAGTCT
    ACCAGCTTTG GGCTGATGCT GACATCATAC ATAGCTTCTT GCTGGAACCA TGATGGGGAC
    CCTGTGGAGC TCTTGAAGTT GGGAAATCAG TTAGCTAAAT TCAGACAGTG CCTGCAGGAA
    AATCCAAAAT TTTTGCAAGA AAAAGTAAAA CAGTATTTTA AGAATAACCA GCATAAGCTG
    ACTTTATCGA TGAGGCCAGA TGACAAGTAT CACGAGAAGC AGGCACAGGT GGAAGCCACG
    AAGCTCAAGC AGAAGGTCGA GGCTCTGTCC CCCGGAGACA GGCAGCAGAT CTACGAGAAA
    GGTCTAGAAT TACGGAGTCA ACAAAGCAAA CCTCAAGATG CCTCTTGTCT GCCAGCGTTG
    AAAGTTTCCG ATATTGAACC CACCATACCT GTCACAGAGT TGGACGTGGT CCTGACAGCT
    GGAGATATCC CTGTTCAGTA CTGCGCCCAG CCCACCAATG GCATGGTGTA TTTCCGGGCC
    TTCTCCAGCC TGAACACACT CCCCGAGGAG CTGAGGCCCT ATGTGCCCCT CTTCTGCAGC
    GTCCTCACCA AGCTGGGCTG CGGCCTTCTT GACTACCGGG AGCAGGCTCA GCAGATAGAA
    TTGAAGACCG GAGGGATGAG TGCTTCTCCC CACGTGCTCC CCGACGACTC ACACATGGAC
    ACCTACGAGC AGGGTGTGCT TTTCTCCTCT CTCTGCCTGG ATCGAAACCT GCCAGACATG
    ATGCAGCTAT GGAGTGAAAT ATTTAACAAC CCGTGCTTTG AAGAAGAGGA GCACTTCAAG
    GTGCTGGTGA AGATGACCGC CCAGGAGCTC GCCAATGGAA TTCCTGACTC TGGGCACCTG
    TACGCATCCA TCAGGGCAGG CCGGACCCTC ACGCCCGCAG GGGACCTGCA GGAGACCTTC
    AGCGGGATGG ATCAGGTGCG GCTGATGAAG AGGATTGCAG AAATGACAGA TATCAAACCC
    ATCCTGAGGA AGCTCCCGCG TATCAAGAAA CACTTGTTAA ATGGTGATAA TATGAGGTGT
    TCAGTGAATG CGACTCCTCA GCAGATGCCT CAGACAGAAA AAGCGGTCGA AGACTTCCTT
    AGAAGCATCG GTCGGAGTAA AAAGGAACGG AGGCCTGTGC GCCCACACAC GGTCGAGAAA
    CCTGTGCCCA GCAGCTCTGG TGGAGATGCC CACGTTCCCC ATGGCTCCCA GGTCATTAGG
    AAGCTGGTCA TGGAACCCAC CTTCAAGCCC TGGCAGATGA AGACTCACTT CCTGATGCCC
    TTCCCGGTGA ATTACGTGGG TGAATGCATC CGAACTGTCC CCTACACGGA CCCAGATCAT
    GCCAGTCTTA AAATCCTTGC ACGTTTGATG ACTGCCAAAT TCTTGCATAC AGAAATTCGA
    GAAAAAGGCG GTGCTTATGG TGGAGGCGCA AAACTCAGCC ACAATGGGAT TTTCACCCTT
    TACTCTTACA GGGACCCAAA TACAATAGAG ACGCTCCAGT CTTTTGGGAA GGCTGTCGAC
    TGGGCTAAGT CTGGAAAATT CACACAGCAA GACATCGACG AAGCCAAACT TTCTGTCTTC
    TCAACCGTAG ATGCTCCTGT CGCTCCTTCA GACAAAGGAA TGGACCACTT CTTGTACGGC
    CTCTCGGATG AGATGAAGCA GGCCCACAGA GAGCAGCTCT TTGCTGTCAG CCACGACAAG
    CTCCTGGCCG TGAGCGATAG ATACCTCGGC ACTGGGAAGA GCACACACGG CCTGGCCATC
    CTCGGACCCG AGAACCCGAA AATTGCCAAG GACCCATCCT GGATCATCCA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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