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SIVA1 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol SIVA1
Entrez Gene ID 10572
Full Name SIVA1 apoptosis inducing factor
Synonyms CD27BP,SIVA,Siva-1,Siva-2
General protein information
Preferred Names

SIVA1 apoptosis inducing factor

Names

apoptosis regulatory protein Siva
CD27-binding (Siva) protein
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

14

14q32.33
Summary This gene encodes a protein with an important role in the apoptotic (programmed cell death) pathway induced by the CD27 antigen, a member of the tumor necrosis factor receptor (TFNR) superfamily. The CD27 antigen cytoplasmic tail binds to the N-terminus of this protein. Two alternatively spliced transcript variants encoding distinct proteins have been described. [provided by RefSeq, Jul 2008].
Disorder MIM:

605567


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_006427.3 NP_006418.2 apoptosis regulatory protein Siva isoform 1
NM_021709.3 NP_068355.1 apoptosis regulatory protein Siva isoform 2
XM_011536360.2 XP_011534662.1 apoptosis regulatory protein Siva isoform X1
NM_021709.2 NP_068355.1 apoptosis regulatory protein Siva isoform 2
NM_006427.4 NP_006418.2 apoptosis regulatory protein Siva isoform 1

Pathways from BioSystems


Homologies


Mus musculus (house mouse) Siva1 NP_038957.2
Gallus gallus (chicken) SIVA1 NP_001264464.1
Bos taurus (cattle) SIVA1 NP_001180107.1
Rattus norvegicus (Norway rat) Siva1 NP_001094452.1
Danio rerio (zebrafish) siva1 XP_700568.6
Canis lupus familiaris (dog) SIVA1 XP_855513.1
Homo sapiens (human) SIVA1 NP_006418.2
Pan troglodytes (chimpanzee) SIVA1 XP_510197.3
SIVA1 XP_001095069.2
Xenopus tropicalis (tropical clawed frog) siva1 NP_001039138.1

Gene Ontology


Bibliography

Related articles in PubMed

E3 ubiquitin ligase Cbl-b activates the p53 pathway by targeting Siva1, a negative regulator of ARF, in FLT3 inhibitor-resistant acute myeloid leukemia.
Park IK, Blum W, Baker SD, Caligiuri MA
Leukemia31(2)502-505(2017 02)

Overexpression and Characterization of The C-Terminal Domain of Human Siva1, A Proapoptotic Factor and Cytoskeleton Binding Protein.
Dantas LE, Saad ST, Ramos CH, B?nichou S
Protein and peptide letters23(1)43-50(2016)

The p53 Target Gene SIVA Enables Non-Small Cell Lung Cancer Development.
Van Nostrand JL, Brisac A, Mello SS, Jacobs SB, Luong R, Attardi LD
Cancer discovery5(6)622-35(2015 Jun)

Anticancer activity of recombinant Siva1 protein in human nasopharyngeal carcinoma cell line CNE-2.
Chen GH, Xue QQ, Li J, Gao TL, Sun QS, Bai GP
Cancer biomarkers : section A of Disease markers15(6)833-41(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings collectively reveal a novel role for the p53 target gene SIVA both in regulating metabolism and in enabling tumorigenesis, independently of p53.
Title: The p53 Target Gene SIVA Enables Non-Small Cell Lung Cancer Development.

electrical stimulation -inducible SIVA1 modulates p53 activities in proliferating keratinocytes, and exogenous electrical stimulation affects p53/HDM2/SIVA1 axis leading to increased proliferation during re-epithelialization
Title: Electrical stimulation enhances epidermal proliferation in human cutaneous wounds by modulating p53-SIVA1 interaction.

Loss of SIVA1 expression is associated with nasopharyngeal carcinoma.
Title: Anticancer activity of recombinant Siva1 protein in human nasopharyngeal carcinoma cell line CNE-2.

SIVA1 interacts with RAD18 and serves as a molecular bridge between RAD18 and PCNA, thus targeting the E3 ligase activity of RAD18 onto PCNA.
Title: SIVA1 directs the E3 ubiquitin ligase RAD18 for PCNA monoubiquitination.

Functional test demonstrated that Siva1 significantly inhibited the invasion and migration of HCT116 cells.
Title: Preliminary research on the expression, purification and function of the apoptotic fusion protein, Sival.

The following SIVA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SIVA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu03722 NM_021709.3
Latest version!		 Homo sapiens SIVA1 apoptosis inducing factor (SIVA1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu18390 NM_006427.4
Latest version!		 Homo sapiens SIVA1 apoptosis inducing factor (SIVA1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu64122 XM_011536360.2
Latest version!		 Homo sapiens SIVA1 apoptosis inducing factor (SIVA1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
View Old Accession Versions >>
OHu03722 NM_021709.2 Homo sapiens SIVA1 apoptosis inducing factor (SIVA1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu18390 NM_006427.3 Homo sapiens SIVA1 apoptosis inducing factor (SIVA1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu03722
Clone ID Related Accession (Same CDS sequence) NM_021709.3 , NM_021709.2
Accession Version NM_021709.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 333bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-09-27
Organism Homo sapiens(human)
Product apoptosis regulatory protein Siva isoform 2
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU195069.1 and BC034562.1. On May 31, 2019 this sequence version replaced NM_021709.2. Transcript Variant: This variant (2) lacks an alternate in-frame segment, compared to variant 1. It appears to lack the apoptotic properties of Siva. The resulting protein (isoform 2) is shorter when it is compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BE047027.1, DRR138523.27114.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
ATGCCCAAGC GGAGCTGCCC CTTCGCGGAC GTGGCCCCGC TACAGCTCAA GGTCCGCGTG 
AGCCAGAGGG AGTTGAGCCG CGGCGTGTGC GCCGAGCGCT ACTCGCAGGA GGTCTTCGAC 
CCATCTGGGG TAGCGTCCAT TGCCTGTTCC TCATGCGTGC GAGCCGTGGA TGGGAAGGCG 
GTCTGCGGTC AGTGTGAGCG AGCCCTGTGC GGGCAGTGTG TGCGCACCTG CTGGGGCTGC 
GGCTCCGTGG CCTGTACCCT GTGTGGCCTC GTGGACTGCA GTGACATGTA CGAGAAAGTG 
CTGTGCACCA GCTGTGCCAT GTTCGAGACC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_068355.1
CDS56..388
Translation

Target ORF information:

RefSeq Version NM_021709.3
Organism Homo sapiens(human)
Definition Homo sapiens SIVA1 apoptosis inducing factor (SIVA1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_021709.3

ORF Insert Sequence:

1
61
121
181
241
301
ATGCCCAAGC GGAGCTGCCC CTTCGCGGAC GTGGCCCCGC TACAGCTCAA GGTCCGCGTG 
AGCCAGAGGG AGTTGAGCCG CGGCGTGTGC GCCGAGCGCT ACTCGCAGGA GGTCTTCGAC 
CCATCTGGGG TAGCGTCCAT TGCCTGTTCC TCATGCGTGC GAGCCGTGGA TGGGAAGGCG 
GTCTGCGGTC AGTGTGAGCG AGCCCTGTGC GGGCAGTGTG TGCGCACCTG CTGGGGCTGC 
GGCTCCGTGG CCTGTACCCT GTGTGGCCTC GTGGACTGCA GTGACATGTA CGAGAAAGTG 
CTGTGCACCA GCTGTGCCAT GTTCGAGACC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu03722
Clone ID Related Accession (Same CDS sequence) NM_021709.3 , NM_021709.2
Accession Version NM_021709.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 333bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-16
Organism Homo sapiens(human)
Product apoptosis regulatory protein Siva isoform 2
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU195069.1 and BC034562.1. On Dec 14, 2006 this sequence version replaced NM_021709.1. Transcript Variant: This variant (2) lacks an alternate in-frame segment, compared to variant 1. It appears to lack the apoptotic properties of Siva. The resulting protein (isoform 2) is shorter when it is compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC034562.1, HX784790.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
ATGCCCAAGC GGAGCTGCCC CTTCGCGGAC GTGGCCCCGC TACAGCTCAA GGTCCGCGTG 
AGCCAGAGGG AGTTGAGCCG CGGCGTGTGC GCCGAGCGCT ACTCGCAGGA GGTCTTCGAC 
CCATCTGGGG TAGCGTCCAT TGCCTGTTCC TCATGCGTGC GAGCCGTGGA TGGGAAGGCG 
GTCTGCGGTC AGTGTGAGCG AGCCCTGTGC GGGCAGTGTG TGCGCACCTG CTGGGGCTGC 
GGCTCCGTGG CCTGTACCCT GTGTGGCCTC GTGGACTGCA GTGACATGTA CGAGAAAGTG 
CTGTGCACCA GCTGTGCCAT GTTCGAGACC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_068355.1
CDS70..402
Translation

Target ORF information:

RefSeq Version NM_021709.2
Organism Homo sapiens(human)
Definition Homo sapiens SIVA1 apoptosis inducing factor (SIVA1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_021709.2

ORF Insert Sequence:

1
61
121
181
241
301
ATGCCCAAGC GGAGCTGCCC CTTCGCGGAC GTGGCCCCGC TACAGCTCAA GGTCCGCGTG 
AGCCAGAGGG AGTTGAGCCG CGGCGTGTGC GCCGAGCGCT ACTCGCAGGA GGTCTTCGAC 
CCATCTGGGG TAGCGTCCAT TGCCTGTTCC TCATGCGTGC GAGCCGTGGA TGGGAAGGCG 
GTCTGCGGTC AGTGTGAGCG AGCCCTGTGC GGGCAGTGTG TGCGCACCTG CTGGGGCTGC 
GGCTCCGTGG CCTGTACCCT GTGTGGCCTC GTGGACTGCA GTGACATGTA CGAGAAAGTG 
CTGTGCACCA GCTGTGCCAT GTTCGAGACC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18390
Clone ID Related Accession (Same CDS sequence) NM_006427.3 , NM_006427.4
Accession Version NM_006427.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 528bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-10-01
Organism Homo sapiens(human)
Product apoptosis regulatory protein Siva isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU195069.1, CK001877.1 and BC034562.1. This sequence is a reference standard in the RefSeqGene project. On Dec 14, 2006 this sequence version replaced NM_006427.2. Transcript Variant: This variant (1) is the predominant and longer transcript. It encodes isoform 1 and includes the apoptotic properties of Siva. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.136925.1, SRR1163655.139255.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
ATGCCCAAGC GGAGCTGCCC CTTCGCGGAC GTGGCCCCGC TACAGCTCAA GGTCCGCGTG 
AGCCAGAGGG AGTTGAGCCG CGGCGTGTGC GCCGAGCGCT ACTCGCAGGA GGTCTTCGAG 
AAGACCAAGC GACTCCTGTT CCTCGGGGCC CAGGCCTACC TGGACCACGT GTGGGATGAA 
GGCTGTGCCG TCGTTCACCT GCCAGAGTCC CCAAAGCCTG GCCCTACAGG GGCCCCGAGG 
GCTGCACGTG GGCAGATGCT GATTGGACCA GACGGCCGCC TGATCAGGAG CCTTGGGCAG 
GCCTCCGAAG CTGACCCATC TGGGGTAGCG TCCATTGCCT GTTCCTCATG CGTGCGAGCC 
GTGGATGGGA AGGCGGTCTG CGGTCAGTGT GAGCGAGCCC TGTGCGGGCA GTGTGTGCGC 
ACCTGCTGGG GCTGCGGCTC CGTGGCCTGT ACCCTGTGTG GCCTCGTGGA CTGCAGTGAC 
ATGTACGAGA AAGTGCTGTG CACCAGCTGT GCCATGTTCG AGACCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_006418.2
CDS70..597
Translation

Target ORF information:

RefSeq Version NM_006427.3
Organism Homo sapiens(human)
Definition Homo sapiens SIVA1 apoptosis inducing factor (SIVA1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_006427.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGCCCAAGC GGAGCTGCCC CTTCGCGGAC GTGGCCCCGC TACAGCTCAA GGTCCGCGTG 
AGCCAGAGGG AGTTGAGCCG CGGCGTGTGC GCCGAGCGCT ACTCGCAGGA GGTCTTCGAG 
AAGACCAAGC GACTCCTGTT CCTCGGGGCC CAGGCCTACC TGGACCACGT GTGGGATGAA 
GGCTGTGCCG TCGTTCACCT GCCAGAGTCC CCAAAGCCTG GCCCTACAGG GGCCCCGAGG 
GCTGCACGTG GGCAGATGCT GATTGGACCA GACGGCCGCC TGATCAGGAG CCTTGGGCAG 
GCCTCCGAAG CTGACCCATC TGGGGTAGCG TCCATTGCCT GTTCCTCATG CGTGCGAGCC 
GTGGATGGGA AGGCGGTCTG CGGTCAGTGT GAGCGAGCCC TGTGCGGGCA GTGTGTGCGC 
ACCTGCTGGG GCTGCGGCTC CGTGGCCTGT ACCCTGTGTG GCCTCGTGGA CTGCAGTGAC 
ATGTACGAGA AAGTGCTGTG CACCAGCTGT GCCATGTTCG AGACCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18390
Clone ID Related Accession (Same CDS sequence) NM_006427.3 , NM_006427.4
Accession Version NM_006427.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 528bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-09-26
Organism Homo sapiens(human)
Product apoptosis regulatory protein Siva isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU195069.1, CK001877.1 and BC034562.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_006427.3. Transcript Variant: This variant (1) is the predominant and longer transcript. It encodes isoform 1 and includes the apoptotic properties of Siva. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK223332.1, BQ943607.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000329967.11/ ENSP00000329213.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
ATGCCCAAGC GGAGCTGCCC CTTCGCGGAC GTGGCCCCGC TACAGCTCAA GGTCCGCGTG 
AGCCAGAGGG AGTTGAGCCG CGGCGTGTGC GCCGAGCGCT ACTCGCAGGA GGTCTTCGAG 
AAGACCAAGC GACTCCTGTT CCTCGGGGCC CAGGCCTACC TGGACCACGT GTGGGATGAA 
GGCTGTGCCG TCGTTCACCT GCCAGAGTCC CCAAAGCCTG GCCCTACAGG GGCCCCGAGG 
GCTGCACGTG GGCAGATGCT GATTGGACCA GACGGCCGCC TGATCAGGAG CCTTGGGCAG 
GCCTCCGAAG CTGACCCATC TGGGGTAGCG TCCATTGCCT GTTCCTCATG CGTGCGAGCC 
GTGGATGGGA AGGCGGTCTG CGGTCAGTGT GAGCGAGCCC TGTGCGGGCA GTGTGTGCGC 
ACCTGCTGGG GCTGCGGCTC CGTGGCCTGT ACCCTGTGTG GCCTCGTGGA CTGCAGTGAC 
ATGTACGAGA AAGTGCTGTG CACCAGCTGT GCCATGTTCG AGACCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_006418.2
CDS56..583
Translation

Target ORF information:

RefSeq Version NM_006427.4
Organism Homo sapiens(human)
Definition Homo sapiens SIVA1 apoptosis inducing factor (SIVA1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_006427.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGCCCAAGC GGAGCTGCCC CTTCGCGGAC GTGGCCCCGC TACAGCTCAA GGTCCGCGTG 
AGCCAGAGGG AGTTGAGCCG CGGCGTGTGC GCCGAGCGCT ACTCGCAGGA GGTCTTCGAG 
AAGACCAAGC GACTCCTGTT CCTCGGGGCC CAGGCCTACC TGGACCACGT GTGGGATGAA 
GGCTGTGCCG TCGTTCACCT GCCAGAGTCC CCAAAGCCTG GCCCTACAGG GGCCCCGAGG 
GCTGCACGTG GGCAGATGCT GATTGGACCA GACGGCCGCC TGATCAGGAG CCTTGGGCAG 
GCCTCCGAAG CTGACCCATC TGGGGTAGCG TCCATTGCCT GTTCCTCATG CGTGCGAGCC 
GTGGATGGGA AGGCGGTCTG CGGTCAGTGT GAGCGAGCCC TGTGCGGGCA GTGTGTGCGC 
ACCTGCTGGG GCTGCGGCTC CGTGGCCTGT ACCCTGTGTG GCCTCGTGGA CTGCAGTGAC 
ATGTACGAGA AAGTGCTGTG CACCAGCTGT GCCATGTTCG AGACCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu64122
Clone ID Related Accession (Same CDS sequence) XM_011536360.2
Accession Version XM_011536360.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 501bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product apoptosis regulatory protein Siva isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011536360.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
ATGCCCAAGC GGAGCTGCCC CTTCGCGGAC GTGGCCCCGC TACAGCTCAA GGTCCGCGTG 
AGCCAGAGGG AGTTGAGCCG CGGCGTGTGC GCCGAGCGCT ACTCGCAGGA GGTCTTCGAG 
AAGACCAAGC GACTCCTGTT CCTCGGGGCC CAGGCCTACC TGGACCACGT GTGGGATGAA 
GGCTGTGCCG TCGTTCACCT GCCAGAGTCC CCAAAGCCTG GCCCTACAGG GGCCCCGAGG 
GCTGCACGTG GGCAGATGCT GATTGGACCA GACGGCCGCC TGATCAGGAG CCTTGGGCAG 
GCCTCCGAAG CTGACCCATC TGGGGTAGCG TCCATTGCCT GTTCCTCATG CGTGCGAGCC 
GTGGATGGGA AGGCGGTCTG CGGTCAGTGT GAGCGAGCCC TGTGCGGGCA GTGTGTGCGC 
ACCTGCTGGG GCTGCGGCTC CGTGGCCTGT ACCCTGTGTG GCCTCGTGGA TCTTTTTAGG 
ACGCTGGTCG TGTTGCCTTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011534662.1
CDS783..1283
Translation

Target ORF information:

RefSeq Version XM_011536360.2
Organism Homo sapiens(human)
Definition Homo sapiens SIVA1 apoptosis inducing factor (SIVA1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011536360.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGCCCAAGC GGAGCTGCCC CTTCGCGGAC GTGGCCCCGC TACAGCTCAA GGTCCGCGTG 
AGCCAGAGGG AGTTGAGCCG CGGCGTGTGC GCCGAGCGCT ACTCGCAGGA GGTCTTCGAG 
AAGACCAAGC GACTCCTGTT CCTCGGGGCC CAGGCCTACC TGGACCACGT GTGGGATGAA 
GGCTGTGCCG TCGTTCACCT GCCAGAGTCC CCAAAGCCTG GCCCTACAGG GGCCCCGAGG 
GCTGCACGTG GGCAGATGCT GATTGGACCA GACGGCCGCC TGATCAGGAG CCTTGGGCAG 
GCCTCCGAAG CTGACCCATC TGGGGTAGCG TCCATTGCCT GTTCCTCATG CGTGCGAGCC 
GTGGATGGGA AGGCGGTCTG CGGTCAGTGT GAGCGAGCCC TGTGCGGGCA GTGTGTGCGC 
ACCTGCTGGG GCTGCGGCTC CGTGGCCTGT ACCCTGTGTG GCCTCGTGGA TCTTTTTAGG 
ACGCTGGTCG TGTTGCCTTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

E3 ubiquitin ligase Cbl-b activates the p53 pathway by targeting Siva1, a negative regulator of ARF, in FLT3 inhibitor-resistant acute myeloid leukemia.
Leukemia31(2)502-505(2017 02)
Park IK,Blum W,Baker SD,Caligiuri MA


book

Overexpression and Characterization of The C-Terminal Domain of Human Siva1, A Proapoptotic Factor and Cytoskeleton Binding Protein.
Protein and peptide letters23(1)43-50(2016)
Dantas LE,Saad ST,Ramos CH,B?nichou S


book

The p53 Target Gene SIVA Enables Non-Small Cell Lung Cancer Development.
Cancer discovery5(6)622-35(2015 Jun)
Van Nostrand JL,Brisac A,Mello SS,Jacobs SB,Luong R,Attardi LD


book

Anticancer activity of recombinant Siva1 protein in human nasopharyngeal carcinoma cell line CNE-2.
Cancer biomarkers : section A of Disease markers15(6)833-41(2015)
Chen GH,Xue QQ,Li J,Gao TL,Sun QS,Bai GP