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NEU3 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol NEU3
Entrez Gene ID 10825
Full Name neuraminidase 3
Synonyms SIAL3
General protein information
Preferred Names

neuraminidase 3

Names

sialidase-3
N-acetyl-alpha-neuraminidase 3
ganglioside sialidase
membrane sialidase
sialidase 3 (membrane sialidase)

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

11

11q13.4

Summary This gene product belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. It is localized in the plasma membrane, and its activity is specific for gangliosides. It may play a role in modulating the ganglioside content of the lipid bilayer. [provided by RefSeq, Jul 2008].
Disorder MIM:

604617


mRNA and Protein(s)

mRNA Protein Name
NM_006656.5 NP_006647.3 sialidase-3



Homo sapiens (human) NEU3 NP_006647.3
Canis lupus familiaris (dog) NEU3 XP_005633558.1
Gallus gallus (chicken) NEU3 XP_428099.4
Danio rerio (zebrafish) neu3.4 NP_001103193.1
Bos taurus (cattle) NEU3 NP_776547.2
Danio rerio (zebrafish) neu3.1 NP_001073133.1
Rattus norvegicus (Norway rat) Neu3 NP_446462.1
Danio rerio (zebrafish) neu3.2 NP_001003644.2
Danio rerio (zebrafish) neu3.5 NP_001103203.1
Macaca mulatta (rhesus monkey) LOC694310 XP_002808128.1
Pan troglodytes (chimpanzee) NEU3 XP_003952031.1
Mus musculus (house mouse) Neu3 NP_057929.1
Danio rerio (zebrafish) neu3.3 NP_001071006.1
Xenopus tropicalis (tropical clawed frog) LOC100496715 XP_002942460.1


Related articles in PubMed

Upregulation of sialidase NEU3 in head and neck squamous cell carcinoma associated with lymph node metastasis.
Shiga K, Takahashi K, Sato I, Kato K, Saijo S, Moriya S, Hosono M, Miyagi T
Cancer science106(11)1544-53(2015 Nov)

Sialidase NEU3 contributes neoplastic potential on colon cancer cells as a key modulator of gangliosides by regulating Wnt signaling.
Takahashi K, Hosono M, Sato I, Hata K, Wada T, Yamaguchi K, Nitta K, Shima H, Miyagi T
International journal of cancer137(7)1560-73(2015 Oct)

Role of plasma-membrane-bound sialidase NEU3 in clathrin-mediated endocytosis.
Rodriguez-Walker M, Vilcaes AA, Garbarino-Pico E, Daniotti JL
The Biochemical journal470(1)131-44(2015 Aug)

NEU3 activity enhances EGFR activation without affecting EGFR expression and acts on its sialylation levels.
Mozzi A, Forcella M, Riva A, Difrancesco C, Molinari F, Martin V, Papini N, Bernasconi B, Nonnis S, Tedeschi G, Mazzucchelli L, Monti E, Fusi P, Frattini M
Glycobiology25(8)855-68(2015 Aug)

Potentiation of epidermal growth factor-mediated oncogenic transformation by sialidase NEU3 leading to Src activation.
Yamamoto K, Takahashi K, Shiozaki K, Yamaguchi K, Moriya S, Hosono M, Shima H, Miyagi T
PloS one10(3)e0120578(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The most potent compound tested targeted the human Neu4 isoenzyme, and was able to substantially reduce the rate of cell migration. We found that the lateral mobility of integrins was reduced by treatment of cells with Neu3, suggesting that Neu3 enzyme activity resulted in changes to integrin-co-receptor or integrin-cytoskeleton interactions
Title: Integrin-mediated cell migration is blocked by inhibitors of human neuraminidase.

NEU3 possesses the ability to interact with specific proteins, many of which are different in each cell compartment, supporting NEU3 involvement in the cell stress response, protein folding, and intracellular trafficking.
Title: NEU3 Sialidase Protein Interactors in the Plasma Membrane and in the Endosomes.

findings identify NEU3 as a participant in head and neck squamous cell carcinoma progression
Title: Upregulation of sialidase NEU3 in head and neck squamous cell carcinoma associated with lymph node metastasis.

In the plasma membrane, NEU3 has a role in endocytosis and protein binding at the cell surface.
Title: Role of plasma-membrane-bound sialidase NEU3 in clathrin-mediated endocytosis.

Besides the already reported indirect EGFR activation through GM3, sialidase NEU3 could also play a role on EGFR activation through its desialylation.
Title: NEU3 activity enhances EGFR activation without affecting EGFR expression and acts on its sialylation levels.

The following NEU3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEU3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu30315 NM_006656.5
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Homo sapiens neuraminidase 3 (NEU3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu30315
Clone ID Related Accession (Same CDS sequence) NM_006656.5
Accession Version NM_006656.5 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1386bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-28
Organism Homo sapiens(Human)
Product sialidase-3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB240271.1, CN429425.1 and AP001992.4. On Nov 1, 2006 this sequence version replaced NM_006656.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DB240271.1, Y18563.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGAGACCTG CGGACCTGCC CCCGCGCCCC ATGGAAGAAT CCCCGGCGTC CAGCTCTGCC 
CCGACAGAGA CGGAGGAGCC GGGGTCCAGT GCAGAGGTCA TGGAAGAAGT GACAACATGC
TCCTTCAACA GCCCTCTGTT CCGGCAGGAA GATGACAGAG GGATTACCTA CCGGATCCCA
GCCCTGCTCT ACATACCCCC CACCCACACC TTCCTGGCCT TTGCAGAGAA GCGTTCTACG
AGGAGAGATG AGGATGCTCT CCACCTGGTG CTGAGGCGAG GGTTGAGGAT TGGGCAGTTG
GTACAGTGGG GGCCCCTGAA GCCACTGATG GAAGCCACAC TACCGGGGCA TCGGACCATG
AACCCCTGTC CTGTATGGGA GCAGAAGAGT GGTTGTGTGT TCCTGTTCTT CATCTGTGTG
CGGGGCCATG TCACAGAGCG TCAACAGATT GTGTCAGGCA GGAATGCTGC CCGCCTTTGC
TTCATCTACA GTCAGGATGC TGGATGTTCA TGGAGTGAGG TGAGGGACTT GACTGAGGAG
GTCATTGGCT CAGAGCTGAA GCACTGGGCC ACATTTGCTG TGGGCCCAGG TCATGGCATC
CAGCTGCAGT CAGGGAGACT GGTCATCCCT GCGTATACCT ACTACATCCC TTCCTGGTTC
TTTTGCTTCC AGCTACCATG TAAAACCAGG CCTCATTCTC TGATGATCTA CAGTGATGAC
CTAGGGGTCA CATGGCACCA TGGTAGACTC ATTAGGCCCA TGGTTACAGT AGAATGTGAA
GTGGCAGAGG TGACTGGGAG GGCTGGCCAC CCTGTGCTAT ATTGCAGTGC CCGGACACCA
AACAGGTGCC GGGCAGAGGC GCTCAGCACT GACCATGGTG AAGGCTTTCA GAGACTGGCC
CTGAGTCGAC AGCTCTGTGA GCCCCCACAT GGTTGCCAAG GGAGTGTGGT AAGTTTCCGG
CCCCTGGAGA TCCCACATAG GTGCCAGGAC TCTAGCAGCA AAGATGCACC CACCATTCAG
CAGAGCTCTC CAGGCAGTTC ACTGAGGCTG GAGGAGGAAG CTGGAACACC GTCAGAATCA
TGGCTCTTGT ACTCACACCC AACCAGTAGG AAACAGAGGG TTGACCTAGG TATCTATCTC
AACCAGACCC CCTTGGAGGC TGCCTGCTGG TCCCGCCCCT GGATCTTGCA CTGTGGGCCC
TGTGGCTACT CTGATCTGGC TGCTCTGGAG GAGGAGGGCT TGTTTGGGTG TTTGTTTGAA
TGTGGGACCA AGCAAGAGTG TGAGCAGATT GCCTTCCGCC TGTTTACACA CCGGGAGATC
CTGAGTCACC TGCAGGGGGA CTGCACCAGC CCTGGTAGGA ACCCAAGCCA ATTCAAAAGC
AATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_006647.3
CDS157..1542
Misc Feature(1)286..1464
Misc Feature(2)325..486
Misc Feature(3)order(328..330,406..408,928..930,988..990,1273..1275,1363..1365,1414..1416)
Misc Feature(4)364..1407
Misc Feature(5)511..687
Misc Feature(6)727..906
Misc Feature(7)925..1053
Misc Feature(8)1087..1344
Misc Feature(9)1360..1464
Exon (1)1..250
Exon (2)251..462
Exon (3)463..2748
Translation

Target ORF information:

RefSeq Version NM_006656.5
Organism Homo sapiens(Human)
Definition Homo sapiens neuraminidase 3 (NEU3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006656.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGAGACCTG CGGACCTGCC CCCGCGCCCC ATGGAAGAAT CCCCGGCGTC CAGCTCTGCC 
CCGACAGAGA CGGAGGAGCC GGGGTCCAGT GCAGAGGTCA TGGAAGAAGT GACAACATGC
TCCTTCAACA GCCCTCTGTT CCGGCAGGAA GATGACAGAG GGATTACCTA CCGGATCCCA
GCCCTGCTCT ACATACCCCC CACCCACACC TTCCTGGCCT TTGCAGAGAA GCGTTCTACG
AGGAGAGATG AGGATGCTCT CCACCTGGTG CTGAGGCGAG GGTTGAGGAT TGGGCAGTTG
GTACAGTGGG GGCCCCTGAA GCCACTGATG GAAGCCACAC TACCGGGGCA TCGGACCATG
AACCCCTGTC CTGTATGGGA GCAGAAGAGT GGTTGTGTGT TCCTGTTCTT CATCTGTGTG
CGGGGCCATG TCACAGAGCG TCAACAGATT GTGTCAGGCA GGAATGCTGC CCGCCTTTGC
TTCATCTACA GTCAGGATGC TGGATGTTCA TGGAGTGAGG TGAGGGACTT GACTGAGGAG
GTCATTGGCT CAGAGCTGAA GCACTGGGCC ACATTTGCTG TGGGCCCAGG TCATGGCATC
CAGCTGCAGT CAGGGAGACT GGTCATCCCT GCGTATACCT ACTACATCCC TTCCTGGTTC
TTTTGCTTCC AGCTACCATG TAAAACCAGG CCTCATTCTC TGATGATCTA CAGTGATGAC
CTAGGGGTCA CATGGCACCA TGGTAGACTC ATTAGGCCCA TGGTTACAGT AGAATGTGAA
GTGGCAGAGG TGACTGGGAG GGCTGGCCAC CCTGTGCTAT ATTGCAGTGC CCGGACACCA
AACAGGTGCC GGGCAGAGGC GCTCAGCACT GACCATGGTG AAGGCTTTCA GAGACTGGCC
CTGAGTCGAC AGCTCTGTGA GCCCCCACAT GGTTGCCAAG GGAGTGTGGT AAGTTTCCGG
CCCCTGGAGA TCCCACATAG GTGCCAGGAC TCTAGCAGCA AAGATGCACC CACCATTCAG
CAGAGCTCTC CAGGCAGTTC ACTGAGGCTG GAGGAGGAAG CTGGAACACC GTCAGAATCA
TGGCTCTTGT ACTCACACCC AACCAGTAGG AAACAGAGGG TTGACCTAGG TATCTATCTC
AACCAGACCC CCTTGGAGGC TGCCTGCTGG TCCCGCCCCT GGATCTTGCA CTGTGGGCCC
TGTGGCTACT CTGATCTGGC TGCTCTGGAG GAGGAGGGCT TGTTTGGGTG TTTGTTTGAA
TGTGGGACCA AGCAAGAGTG TGAGCAGATT GCCTTCCGCC TGTTTACACA CCGGGAGATC
CTGAGTCACC TGCAGGGGGA CTGCACCAGC CCTGGTAGGA ACCCAAGCCA ATTCAAAAGC
AATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Upregulation of sialidase NEU3 in head and neck squamous cell carcinoma associated with lymph node metastasis.
Cancer science106(11)1544-53(2015 Nov)
Shiga K,Takahashi K,Sato I,Kato K,Saijo S,Moriya S,Hosono M,Miyagi T


book

Sialidase NEU3 contributes neoplastic potential on colon cancer cells as a key modulator of gangliosides by regulating Wnt signaling.
International journal of cancer137(7)1560-73(2015 Oct)
Takahashi K,Hosono M,Sato I,Hata K,Wada T,Yamaguchi K,Nitta K,Shima H,Miyagi T


book

Role of plasma-membrane-bound sialidase NEU3 in clathrin-mediated endocytosis.
The Biochemical journal470(1)131-44(2015 Aug)
Rodriguez-Walker M,Vilcaes AA,Garbarino-Pico E,Daniotti JL


book

NEU3 activity enhances EGFR activation without affecting EGFR expression and acts on its sialylation levels.
Glycobiology25(8)855-68(2015 Aug)
Mozzi A,Forcella M,Riva A,Difrancesco C,Molinari F,Martin V,Papini N,Bernasconi B,Nonnis S,Tedeschi G,Mazzucchelli L,Monti E,Fusi P,Frattini M


book

Potentiation of epidermal growth factor-mediated oncogenic transformation by sialidase NEU3 leading to Src activation.
PloS one10(3)e0120578(2015)
Yamamoto K,Takahashi K,Shiozaki K,Yamaguchi K,Moriya S,Hosono M,Shima H,Miyagi T


 
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