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SUGT1 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol SUGT1
Entrez Gene ID 10910
Full Name SGT1 homolog, MIS12 kinetochore complex assembly cochaperone
Synonyms SGT1
General protein information
Preferred Names

SGT1 homolog, MIS12 kinetochore complex assembly cochaperone

Names

protein SGT1 homolog
SGT1, suppressor of G2 allele of SKP1
putative 40-6-3 protein
suppressor of G2 allele of SKP1, S. cerevisiae, homolog of
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

13

13q14.3
Summary This gene encodes a highly conserved nuclear protein involved in kinetochore function and required for the G1/S and G2/M transitions. This protein interacts with heat shock protein 90. Alternative splicing results in multiple transcript variants. Pseudogenes for this gene have been defined on several different chromosomes. [provided by RefSeq, Mar 2016].
Disorder MIM:

604098


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001130912.1 NP_001124384.1 protein SGT1 homolog isoform SGT1B
XM_017020366.1 XP_016875855.1 protein SGT1 homolog isoform X2
NM_001130912.3 NP_001124384.1 protein SGT1 homolog isoform B
NM_006704.5 NP_006695.1 protein SGT1 homolog isoform A
XM_017020367.1 XP_016875856.1 protein SGT1 homolog isoform X2
NM_001130912.2 NP_001124384.1 protein SGT1 homolog isoform B
XM_011534893.2 XP_011533195.1 protein SGT1 homolog isoform X5
NM_006704.3 NP_006695.1 protein SGT1 homolog isoform SGT1A
NM_001320831.1 NP_001307760.1 protein SGT1 homolog isoform C
NM_001320831.2 NP_001307760.1 protein SGT1 homolog isoform C
XM_017020368.1 XP_016875857.1 protein SGT1 homolog isoform X4
NM_006704.4 NP_006695.1 protein SGT1 homolog isoform A
XM_017020366.2 XP_016875855.1 protein SGT1 homolog isoform X2
XM_017020365.1 XP_016875854.1 protein SGT1 homolog isoform X1
XM_011534891.2 XP_011533193.1 protein SGT1 homolog isoform X3
XM_011534893.3 XP_011533195.1 protein SGT1 homolog isoform X5

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) SUGT1 XP_003952448.1
Arabidopsis thaliana (thale cress) SGT1A NP_849429.1
Oryza sativa (rice) Os01g0624500 NP_001043620.1
Canis lupus familiaris (dog) SUGT1 XP_005633996.1
Mus musculus (house mouse) Sugt1 NP_080750.1
Rattus norvegicus (Norway rat) Sugt1 NP_001013069.1
Arabidopsis thaliana (thale cress) SGT1B NP_192865.1
Bos taurus (cattle) SUGT1 NP_001039668.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP006161 XP_316219.4
Xenopus tropicalis (tropical clawed frog) sugt1 NP_001016156.1
Homo sapiens (human) SUGT1 NP_001124384.1
SUGT1 XP_001084394.1
Gallus gallus (chicken) SUGT1 NP_001025994.2
Danio rerio (zebrafish) sgut1 NP_001007362.1
Drosophila melanogaster (fruit fly) Sgt1 NP_649783.2
Caenorhabditis elegans (roundworm) CELE_D1054.3 NP_505751.1

Gene Ontology


Bibliography

Related articles in PubMed

SGT1-HSP90 complex is required for CENP-A deposition at centromeres.
Niikura Y, Kitagawa R, Ogi H, Kitagawa K
Cell cycle (Georgetown, Tex.)16(18)1683-1694(2017 Sep)

Interplay between the phosphatase PHLPP1 and E3 ligase RNF41 stimulates proper kinetochore assembly via the outer-kinetochore protein SGT1.
Gangula NR, Maddika S
The Journal of biological chemistry292(34)13947-13958(2017 08)

Application of SGT1-Hsp90 chaperone complex for soluble expression of NOD1 LRR domain in E.?coli.
Hong TJ, Hahn JS
Biochemical and biophysical research communications478(4)1647-52(2016 09)

Translation elongation factor eEF1A1 is a novel partner of a multifunctional protein Sgt1.
Novosylna O, Jurewicz E, Pydiura N, Goral A, Filipek A, Negrutskii B, El'skaya A
Biochimie119137-45(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that PHLPP1 plays an important role in assembly of kinetochores by counteracting RNF41-mediated SGT1 degradation. (PHLPP1 = PH domain and leucine rich repeat protein phosphatase 1; RNF41 = ring finger protein 41; SGT1 = suppressor of G2 allele of SKP1)
Title: Interplay between the phosphatase PHLPP1 and E3 ligase RNF41 stimulates proper kinetochore assembly via the outer-kinetochore protein SGT1.

Fusion of human SGT1 (hSGT1) to NOD1 LRR significantly enhanced the solubility, and the fusion protein was stabilized by coexpression of mouse Hsp90alpha.
Title: Application of SGT1-Hsp90 chaperone complex for soluble expression of NOD1 LRR domain in E.?coli.

Data (including data from studies using purified proteins/hepatocyte lysates) suggest eEF1A1/Sgt1a interact as multimer; D2/D3 domains of eEF1A1 and TPR domain of Sgt1 are involved in multimer formation; Sgt1 competes with viral RNA to bind to eEF1A.
Title: Translation elongation factor eEF1A1 is a novel partner of a multifunctional protein Sgt1.

our data revealed a previously uncovered role of SGT1 in gastric cancer development, and suggested that SGT1 could be a promising anti-cancer target to against gastric cancer
Title: SGT1 regulates Akt signaling by promoting beta-TrCP-dependent PHLPP1 degradation in gastric cancer cells.

Data suggest that SspH2 (ubiquitin ligase effector from Salmonella typhimurium) ubiquitination activity and protein stability is enhanced by SGT1.
Title: The Salmonella type III effector SspH2 specifically exploits the NLR co-chaperone activity of SGT1 to subvert immunity.

The following SUGT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SUGT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu09232 NM_006704.5
Latest version!		 Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $159.50-$223.30
$319.00
OHu19340 NM_001130912.3
Latest version!		 Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $159.50-$223.30
$319.00
OHu64514 XM_011534893.3
Latest version!		 Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $139.30
$199.00
OHu64513 NM_001320831.2
Latest version!		 Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu64512 XM_011534891.2
Latest version!		 Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu84409 XM_017020365.1
Latest version!		 Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
OHu84410 XM_017020367.1
Latest version!		 Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00
OHu84410 XM_017020366.2
Latest version!		 Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00
OHu84411 XM_017020368.1
Latest version!		 Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00
View Old Accession Versions >>
OHu19340 NM_001130912.1 Homo sapiens SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae) (SUGT1), transcript variant 1B, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $159.50-$223.30
$319.00
OHu19340 NM_001130912.2 Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $159.50-$223.30
$319.00
OHu09232 NM_006704.3 Homo sapiens SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae) (SUGT1), transcript variant 1A, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $159.50-$223.30
$319.00
OHu64513 NM_001320831.1 Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu09232 NM_006704.4 Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $159.50-$223.30
$319.00
OHu84410 XM_017020366.1 Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00
OHu64514 XM_011534893.2 Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $139.30
$199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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2-4 Clone 40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu09232
Clone ID Related Accession (Same CDS sequence) NM_006704.5 , NM_006704.3 , NM_006704.4
Accession Version NM_006704.5 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1002bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-26
Organism Homo sapiens(human)
Product protein SGT1 homolog isoform A
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL139089.13. On Jun 2, 2019 this sequence version replaced NM_006704.4. Transcript Variant: This variant (2, also known as A) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (A) is shorter than isoform B. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.91720.1, SRR1803614.129272.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
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541
601
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721
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901
961
ATGGCGGCGG CTGCAGCAGG AACTGCAACA TCCCAGAGGT TTTTCCAGAG CTTCTCGGAT 
GCCCTAATCG ACGAGGACCC CCAGGCGGCG TTAGAGGAGC TGACTAAGGC TTTGGAACAG 
AAACCAGATG ATGCACAGTA TTATTGTCAA AGAGCTTATT GTCACATTCT TCTTGGGAAT 
TACTGTGTTG CTGTTGCTGA TGCAAAGAAG TCTCTAGAAC TCAATCCAAA TAATTCCACT 
GCTATGCTGA GAAAAGGAAT ATGTGAATAC CATGAAAAAA ACTATGCTGC TGCCCTAGAA 
ACTTTTACAG AAGGACAAAA ATTAGATAGT GCAGATGCTA ATTTCAGTGT CTGGATTAAA 
AGGTGTCAAG AAGCTCAGAA TGGCTCAGAA TCTGAGGTGT GGACTCATCA GTCAAAAATC 
AAGTATGACT GGTATCAAAC AGAATCTCAA GTAGTCATTA CACTTATGAT CAAGAATGTT 
CAGAAGAATG ATGTAAATGT GGAATTTTCA GAAAAAGAGT TGTCTGCTTT GGTTAAACTT 
CCTTCTGGAG AGGATTACAA TTTGAAACTG GAACTTCTTC ATCCTATAAT ACCAGAACAG 
AGCACGTTTA AAGTACTTTC AACAAAGATT GAAATTAAAC TGAAAAAGCC AGAGGCTGTG 
AGATGGGAAA AGCTAGAGGG GCAAGGAGAT GTGCCTACGC CAAAACAATT CGTAGCAGAT 
GTAAAGAACC TATATCCATC ATCATCTCCT TATACAAGAA ATTGGGATAA ATTGGTTGGT 
GAGATCAAAG AAGAAGAAAA GAATGAAAAG TTGGAGGGAG ATGCAGCTTT AAACAGATTA 
TTTCAGCAGA TCTATTCAGA TGGTTCTGAT GAAGTGAAAC GTGCCATGAA CAAATCCTTT 
ATGGAGTCGG GTGGTACAGT TTTGAGTACC AACTGGTCTG ATGTAGGTAA AAGGAAAGTT 
GAAATCAATC CTCCTGATGA TATGGAATGG AAAAAGTACT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_006695.1
CDS86..1087
Translation

Target ORF information:

RefSeq Version NM_006704.5
Organism Homo sapiens(human)
Definition Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_006704.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCGGCGG CTGCAGCAGG AACTGCAACA TCCCAGAGGT TTTTCCAGAG CTTCTCGGAT 
GCCCTAATCG ACGAGGACCC CCAGGCGGCG TTAGAGGAGC TGACTAAGGC TTTGGAACAG 
AAACCAGATG ATGCACAGTA TTATTGTCAA AGAGCTTATT GTCACATTCT TCTTGGGAAT 
TACTGTGTTG CTGTTGCTGA TGCAAAGAAG TCTCTAGAAC TCAATCCAAA TAATTCCACT 
GCTATGCTGA GAAAAGGAAT ATGTGAATAC CATGAAAAAA ACTATGCTGC TGCCCTAGAA 
ACTTTTACAG AAGGACAAAA ATTAGATAGT GCAGATGCTA ATTTCAGTGT CTGGATTAAA 
AGGTGTCAAG AAGCTCAGAA TGGCTCAGAA TCTGAGGTGT GGACTCATCA GTCAAAAATC 
AAGTATGACT GGTATCAAAC AGAATCTCAA GTAGTCATTA CACTTATGAT CAAGAATGTT 
CAGAAGAATG ATGTAAATGT GGAATTTTCA GAAAAAGAGT TGTCTGCTTT GGTTAAACTT 
CCTTCTGGAG AGGATTACAA TTTGAAACTG GAACTTCTTC ATCCTATAAT ACCAGAACAG 
AGCACGTTTA AAGTACTTTC AACAAAGATT GAAATTAAAC TGAAAAAGCC AGAGGCTGTG 
AGATGGGAAA AGCTAGAGGG GCAAGGAGAT GTGCCTACGC CAAAACAATT CGTAGCAGAT 
GTAAAGAACC TATATCCATC ATCATCTCCT TATACAAGAA ATTGGGATAA ATTGGTTGGT 
GAGATCAAAG AAGAAGAAAA GAATGAAAAG TTGGAGGGAG ATGCAGCTTT AAACAGATTA 
TTTCAGCAGA TCTATTCAGA TGGTTCTGAT GAAGTGAAAC GTGCCATGAA CAAATCCTTT 
ATGGAGTCGG GTGGTACAGT TTTGAGTACC AACTGGTCTG ATGTAGGTAA AAGGAAAGTT 
GAAATCAATC CTCCTGATGA TATGGAATGG AAAAAGTACT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu09232
Clone ID Related Accession (Same CDS sequence) NM_006704.5 , NM_006704.3 , NM_006704.4
Accession Version NM_006704.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1002bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-08-08
Organism Homo sapiens(Human)
Product protein SGT1 homolog isoform SGT1A
Comment 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCGGCGG CTGCAGCAGG AACTGCAACA TCCCAGAGGT TTTTCCAGAG CTTCTCGGAT 
GCCCTAATCG ACGAGGACCC CCAGGCGGCG TTAGAGGAGC TGACTAAGGC TTTGGAACAG 
AAACCAGATG ATGCACAGTA TTATTGTCAA AGAGCTTATT GTCACATTCT TCTTGGGAAT 
TACTGTGTTG CTGTTGCTGA TGCAAAGAAG TCTCTAGAAC TCAATCCAAA TAATTCCACT 
GCTATGCTGA GAAAAGGAAT ATGTGAATAC CATGAAAAAA ACTATGCTGC TGCCCTAGAA 
ACTTTTACAG AAGGACAAAA ATTAGATAGT GCAGATGCTA ATTTCAGTGT CTGGATTAAA 
AGGTGTCAAG AAGCTCAGAA TGGCTCAGAA TCTGAGGTGT GGACTCATCA GTCAAAAATC 
AAGTATGACT GGTATCAAAC AGAATCTCAA GTAGTCATTA CACTTATGAT CAAGAATGTT 
CAGAAGAATG ATGTAAATGT GGAATTTTCA GAAAAAGAGT TGTCTGCTTT GGTTAAACTT 
CCTTCTGGAG AGGATTACAA TTTGAAACTG GAACTTCTTC ATCCTATAAT ACCAGAACAG 
AGCACGTTTA AAGTACTTTC AACAAAGATT GAAATTAAAC TGAAAAAGCC AGAGGCTGTG 
AGATGGGAAA AGCTAGAGGG GCAAGGAGAT GTGCCTACGC CAAAACAATT CGTAGCAGAT 
GTAAAGAACC TATATCCATC ATCATCTCCT TATACAAGAA ATTGGGATAA ATTGGTTGGT 
GAGATCAAAG AAGAAGAAAA GAATGAAAAG TTGGAGGGAG ATGCAGCTTT AAACAGATTA 
TTTCAGCAGA TCTATTCAGA TGGTTCTGAT GAAGTGAAAC GTGCCATGAA CAAATCCTTT 
ATGGAGTCGG GTGGTACAGT TTTGAGTACC AACTGGTCTG ATGTAGGTAA AAGGAAAGTT 
GAAATCAATC CTCCTGATGA TATGGAATGG AAAAAGTACT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_006695.1
Translation

Target ORF information:

RefSeq Version NM_006704.3
Organism Homo sapiens(Human)
Definition Homo sapiens SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae) (SUGT1), transcript variant 1A, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_006704.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCGGCGG CTGCAGCAGG AACTGCAACA TCCCAGAGGT TTTTCCAGAG CTTCTCGGAT 
GCCCTAATCG ACGAGGACCC CCAGGCGGCG TTAGAGGAGC TGACTAAGGC TTTGGAACAG 
AAACCAGATG ATGCACAGTA TTATTGTCAA AGAGCTTATT GTCACATTCT TCTTGGGAAT 
TACTGTGTTG CTGTTGCTGA TGCAAAGAAG TCTCTAGAAC TCAATCCAAA TAATTCCACT 
GCTATGCTGA GAAAAGGAAT ATGTGAATAC CATGAAAAAA ACTATGCTGC TGCCCTAGAA 
ACTTTTACAG AAGGACAAAA ATTAGATAGT GCAGATGCTA ATTTCAGTGT CTGGATTAAA 
AGGTGTCAAG AAGCTCAGAA TGGCTCAGAA TCTGAGGTGT GGACTCATCA GTCAAAAATC 
AAGTATGACT GGTATCAAAC AGAATCTCAA GTAGTCATTA CACTTATGAT CAAGAATGTT 
CAGAAGAATG ATGTAAATGT GGAATTTTCA GAAAAAGAGT TGTCTGCTTT GGTTAAACTT 
CCTTCTGGAG AGGATTACAA TTTGAAACTG GAACTTCTTC ATCCTATAAT ACCAGAACAG 
AGCACGTTTA AAGTACTTTC AACAAAGATT GAAATTAAAC TGAAAAAGCC AGAGGCTGTG 
AGATGGGAAA AGCTAGAGGG GCAAGGAGAT GTGCCTACGC CAAAACAATT CGTAGCAGAT 
GTAAAGAACC TATATCCATC ATCATCTCCT TATACAAGAA ATTGGGATAA ATTGGTTGGT 
GAGATCAAAG AAGAAGAAAA GAATGAAAAG TTGGAGGGAG ATGCAGCTTT AAACAGATTA 
TTTCAGCAGA TCTATTCAGA TGGTTCTGAT GAAGTGAAAC GTGCCATGAA CAAATCCTTT 
ATGGAGTCGG GTGGTACAGT TTTGAGTACC AACTGGTCTG ATGTAGGTAA AAGGAAAGTT 
GAAATCAATC CTCCTGATGA TATGGAATGG AAAAAGTACT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu09232
Clone ID Related Accession (Same CDS sequence) NM_006704.5 , NM_006704.3 , NM_006704.4
Accession Version NM_006704.4 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1002bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-06-02
Organism Homo sapiens(human)
Product protein SGT1 homolog isoform A
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN345940.1, BC000911.1 and AK292128.1. On Mar 9, 2016 this sequence version replaced NM_006704.3. Transcript Variant: This variant (2, also known as A) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (A) is shorter than isoform B. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292128.1, SRR1660803.100103.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCGGCGG CTGCAGCAGG AACTGCAACA TCCCAGAGGT TTTTCCAGAG CTTCTCGGAT 
GCCCTAATCG ACGAGGACCC CCAGGCGGCG TTAGAGGAGC TGACTAAGGC TTTGGAACAG 
AAACCAGATG ATGCACAGTA TTATTGTCAA AGAGCTTATT GTCACATTCT TCTTGGGAAT 
TACTGTGTTG CTGTTGCTGA TGCAAAGAAG TCTCTAGAAC TCAATCCAAA TAATTCCACT 
GCTATGCTGA GAAAAGGAAT ATGTGAATAC CATGAAAAAA ACTATGCTGC TGCCCTAGAA 
ACTTTTACAG AAGGACAAAA ATTAGATAGT GCAGATGCTA ATTTCAGTGT CTGGATTAAA 
AGGTGTCAAG AAGCTCAGAA TGGCTCAGAA TCTGAGGTGT GGACTCATCA GTCAAAAATC 
AAGTATGACT GGTATCAAAC AGAATCTCAA GTAGTCATTA CACTTATGAT CAAGAATGTT 
CAGAAGAATG ATGTAAATGT GGAATTTTCA GAAAAAGAGT TGTCTGCTTT GGTTAAACTT 
CCTTCTGGAG AGGATTACAA TTTGAAACTG GAACTTCTTC ATCCTATAAT ACCAGAACAG 
AGCACGTTTA AAGTACTTTC AACAAAGATT GAAATTAAAC TGAAAAAGCC AGAGGCTGTG 
AGATGGGAAA AGCTAGAGGG GCAAGGAGAT GTGCCTACGC CAAAACAATT CGTAGCAGAT 
GTAAAGAACC TATATCCATC ATCATCTCCT TATACAAGAA ATTGGGATAA ATTGGTTGGT 
GAGATCAAAG AAGAAGAAAA GAATGAAAAG TTGGAGGGAG ATGCAGCTTT AAACAGATTA 
TTTCAGCAGA TCTATTCAGA TGGTTCTGAT GAAGTGAAAC GTGCCATGAA CAAATCCTTT 
ATGGAGTCGG GTGGTACAGT TTTGAGTACC AACTGGTCTG ATGTAGGTAA AAGGAAAGTT 
GAAATCAATC CTCCTGATGA TATGGAATGG AAAAAGTACT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_006695.1
CDS231..1232
Translation

Target ORF information:

RefSeq Version NM_006704.4
Organism Homo sapiens(human)
Definition Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_006704.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCGGCGG CTGCAGCAGG AACTGCAACA TCCCAGAGGT TTTTCCAGAG CTTCTCGGAT 
GCCCTAATCG ACGAGGACCC CCAGGCGGCG TTAGAGGAGC TGACTAAGGC TTTGGAACAG 
AAACCAGATG ATGCACAGTA TTATTGTCAA AGAGCTTATT GTCACATTCT TCTTGGGAAT 
TACTGTGTTG CTGTTGCTGA TGCAAAGAAG TCTCTAGAAC TCAATCCAAA TAATTCCACT 
GCTATGCTGA GAAAAGGAAT ATGTGAATAC CATGAAAAAA ACTATGCTGC TGCCCTAGAA 
ACTTTTACAG AAGGACAAAA ATTAGATAGT GCAGATGCTA ATTTCAGTGT CTGGATTAAA 
AGGTGTCAAG AAGCTCAGAA TGGCTCAGAA TCTGAGGTGT GGACTCATCA GTCAAAAATC 
AAGTATGACT GGTATCAAAC AGAATCTCAA GTAGTCATTA CACTTATGAT CAAGAATGTT 
CAGAAGAATG ATGTAAATGT GGAATTTTCA GAAAAAGAGT TGTCTGCTTT GGTTAAACTT 
CCTTCTGGAG AGGATTACAA TTTGAAACTG GAACTTCTTC ATCCTATAAT ACCAGAACAG 
AGCACGTTTA AAGTACTTTC AACAAAGATT GAAATTAAAC TGAAAAAGCC AGAGGCTGTG 
AGATGGGAAA AGCTAGAGGG GCAAGGAGAT GTGCCTACGC CAAAACAATT CGTAGCAGAT 
GTAAAGAACC TATATCCATC ATCATCTCCT TATACAAGAA ATTGGGATAA ATTGGTTGGT 
GAGATCAAAG AAGAAGAAAA GAATGAAAAG TTGGAGGGAG ATGCAGCTTT AAACAGATTA 
TTTCAGCAGA TCTATTCAGA TGGTTCTGAT GAAGTGAAAC GTGCCATGAA CAAATCCTTT 
ATGGAGTCGG GTGGTACAGT TTTGAGTACC AACTGGTCTG ATGTAGGTAA AAGGAAAGTT 
GAAATCAATC CTCCTGATGA TATGGAATGG AAAAAGTACT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19340
Clone ID Related Accession (Same CDS sequence) NM_001130912.1 , NM_001130912.3 , NM_001130912.2
Accession Version NM_001130912.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1098bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-08-08
Organism Homo sapiens(Human)
Product protein SGT1 homolog isoform SGT1B
Comment 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCGGCGG CTGCAGCAGG AACTGCAACA TCCCAGAGGT TTTTCCAGAG CTTCTCGGAT 
GCCCTAATCG ACGAGGACCC CCAGGCGGCG TTAGAGGAGC TGACTAAGGC TTTGGAACAG 
AAACCAGATG ATGCACAGTA TTATTGTCAA AGAGCTTATT GTCACATTCT TCTTGGGAAT 
TACTGTGTTG CTGTTGCTGA TGCAAAGAAG TCTCTAGAAC TCAATCCAAA TAATTCCACT 
GCTATGCTGA GAAAAGGAAT ATGTGAATAC CATGAAAAAA ACTATGCTGC TGCCCTAGAA 
ACTTTTACAG AAGGACAAAA ATTAGATATA GAGACGGGGT TTCATCGTGT TGGCCAGGCT 
GGTCTCCAAC TCTTGACCTC AAGTGATCCA CCTGCCTTGG ACTCCCAAAG TGCTGGGATT 
ACAGGTGCAG ATGCTAATTT CAGTGTCTGG ATTAAAAGGT GTCAAGAAGC TCAGAATGGC 
TCAGAATCTG AGGTGTGGAC TCATCAGTCA AAAATCAAGT ATGACTGGTA TCAAACAGAA 
TCTCAAGTAG TCATTACACT TATGATCAAG AATGTTCAGA AGAATGATGT AAATGTGGAA 
TTTTCAGAAA AAGAGTTGTC TGCTTTGGTT AAACTTCCTT CTGGAGAGGA TTACAATTTG 
AAACTGGAAC TTCTTCATCC TATAATACCA GAACAGAGCA CGTTTAAAGT ACTTTCAACA 
AAGATTGAAA TTAAACTGAA AAAGCCAGAG GCTGTGAGAT GGGAAAAGCT AGAGGGGCAA 
GGAGATGTGC CTACGCCAAA ACAATTCGTA GCAGATGTAA AGAACCTATA TCCATCATCA 
TCTCCTTATA CAAGAAATTG GGATAAATTG GTTGGTGAGA TCAAAGAAGA AGAAAAGAAT 
GAAAAGTTGG AGGGAGATGC AGCTTTAAAC AGATTATTTC AGCAGATCTA TTCAGATGGT 
TCTGATGAAG TGAAACGTGC CATGAACAAA TCCTTTATGG AGTCGGGTGG TACAGTTTTG 
AGTACCAACT GGTCTGATGT AGGTAAAAGG AAAGTTGAAA TCAATCCTCC TGATGATATG 
GAATGGAAAA AGTACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001124384.1
Translation

Target ORF information:

RefSeq Version NM_001130912.1
Organism Homo sapiens(Human)
Definition Homo sapiens SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae) (SUGT1), transcript variant 1B, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001130912.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCGGCGG CTGCAGCAGG AACTGCAACA TCCCAGAGGT TTTTCCAGAG CTTCTCGGAT 
GCCCTAATCG ACGAGGACCC CCAGGCGGCG TTAGAGGAGC TGACTAAGGC TTTGGAACAG 
AAACCAGATG ATGCACAGTA TTATTGTCAA AGAGCTTATT GTCACATTCT TCTTGGGAAT 
TACTGTGTTG CTGTTGCTGA TGCAAAGAAG TCTCTAGAAC TCAATCCAAA TAATTCCACT 
GCTATGCTGA GAAAAGGAAT ATGTGAATAC CATGAAAAAA ACTATGCTGC TGCCCTAGAA 
ACTTTTACAG AAGGACAAAA ATTAGATATA GAGACGGGGT TTCATCGTGT TGGCCAGGCT 
GGTCTCCAAC TCTTGACCTC AAGTGATCCA CCTGCCTTGG ACTCCCAAAG TGCTGGGATT 
ACAGGTGCAG ATGCTAATTT CAGTGTCTGG ATTAAAAGGT GTCAAGAAGC TCAGAATGGC 
TCAGAATCTG AGGTGTGGAC TCATCAGTCA AAAATCAAGT ATGACTGGTA TCAAACAGAA 
TCTCAAGTAG TCATTACACT TATGATCAAG AATGTTCAGA AGAATGATGT AAATGTGGAA 
TTTTCAGAAA AAGAGTTGTC TGCTTTGGTT AAACTTCCTT CTGGAGAGGA TTACAATTTG 
AAACTGGAAC TTCTTCATCC TATAATACCA GAACAGAGCA CGTTTAAAGT ACTTTCAACA 
AAGATTGAAA TTAAACTGAA AAAGCCAGAG GCTGTGAGAT GGGAAAAGCT AGAGGGGCAA 
GGAGATGTGC CTACGCCAAA ACAATTCGTA GCAGATGTAA AGAACCTATA TCCATCATCA 
TCTCCTTATA CAAGAAATTG GGATAAATTG GTTGGTGAGA TCAAAGAAGA AGAAAAGAAT 
GAAAAGTTGG AGGGAGATGC AGCTTTAAAC AGATTATTTC AGCAGATCTA TTCAGATGGT 
TCTGATGAAG TGAAACGTGC CATGAACAAA TCCTTTATGG AGTCGGGTGG TACAGTTTTG 
AGTACCAACT GGTCTGATGT AGGTAAAAGG AAAGTTGAAA TCAATCCTCC TGATGATATG 
GAATGGAAAA AGTACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19340
Clone ID Related Accession (Same CDS sequence) NM_001130912.1 , NM_001130912.3 , NM_001130912.2
Accession Version NM_001130912.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1098bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-26
Organism Homo sapiens(human)
Product protein SGT1 homolog isoform B
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL139089.13. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001130912.2. Transcript Variant: This variant (1, also known as B or SGT1.2) encodes the longest isoform (B). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.31332.1, SRR1803614.94755.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCGGCGG CTGCAGCAGG AACTGCAACA TCCCAGAGGT TTTTCCAGAG CTTCTCGGAT 
GCCCTAATCG ACGAGGACCC CCAGGCGGCG TTAGAGGAGC TGACTAAGGC TTTGGAACAG 
AAACCAGATG ATGCACAGTA TTATTGTCAA AGAGCTTATT GTCACATTCT TCTTGGGAAT 
TACTGTGTTG CTGTTGCTGA TGCAAAGAAG TCTCTAGAAC TCAATCCAAA TAATTCCACT 
GCTATGCTGA GAAAAGGAAT ATGTGAATAC CATGAAAAAA ACTATGCTGC TGCCCTAGAA 
ACTTTTACAG AAGGACAAAA ATTAGATATA GAGACGGGGT TTCATCGTGT TGGCCAGGCT 
GGTCTCCAAC TCTTGACCTC AAGTGATCCA CCTGCCTTGG ACTCCCAAAG TGCTGGGATT 
ACAGGTGCAG ATGCTAATTT CAGTGTCTGG ATTAAAAGGT GTCAAGAAGC TCAGAATGGC 
TCAGAATCTG AGGTGTGGAC TCATCAGTCA AAAATCAAGT ATGACTGGTA TCAAACAGAA 
TCTCAAGTAG TCATTACACT TATGATCAAG AATGTTCAGA AGAATGATGT AAATGTGGAA 
TTTTCAGAAA AAGAGTTGTC TGCTTTGGTT AAACTTCCTT CTGGAGAGGA TTACAATTTG 
AAACTGGAAC TTCTTCATCC TATAATACCA GAACAGAGCA CGTTTAAAGT ACTTTCAACA 
AAGATTGAAA TTAAACTGAA AAAGCCAGAG GCTGTGAGAT GGGAAAAGCT AGAGGGGCAA 
GGAGATGTGC CTACGCCAAA ACAATTCGTA GCAGATGTAA AGAACCTATA TCCATCATCA 
TCTCCTTATA CAAGAAATTG GGATAAATTG GTTGGTGAGA TCAAAGAAGA AGAAAAGAAT 
GAAAAGTTGG AGGGAGATGC AGCTTTAAAC AGATTATTTC AGCAGATCTA TTCAGATGGT 
TCTGATGAAG TGAAACGTGC CATGAACAAA TCCTTTATGG AGTCGGGTGG TACAGTTTTG 
AGTACCAACT GGTCTGATGT AGGTAAAAGG AAAGTTGAAA TCAATCCTCC TGATGATATG 
GAATGGAAAA AGTACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001124384.1
CDS86..1183
Translation

Target ORF information:

RefSeq Version NM_001130912.3
Organism Homo sapiens(human)
Definition Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001130912.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCGGCGG CTGCAGCAGG AACTGCAACA TCCCAGAGGT TTTTCCAGAG CTTCTCGGAT 
GCCCTAATCG ACGAGGACCC CCAGGCGGCG TTAGAGGAGC TGACTAAGGC TTTGGAACAG 
AAACCAGATG ATGCACAGTA TTATTGTCAA AGAGCTTATT GTCACATTCT TCTTGGGAAT 
TACTGTGTTG CTGTTGCTGA TGCAAAGAAG TCTCTAGAAC TCAATCCAAA TAATTCCACT 
GCTATGCTGA GAAAAGGAAT ATGTGAATAC CATGAAAAAA ACTATGCTGC TGCCCTAGAA 
ACTTTTACAG AAGGACAAAA ATTAGATATA GAGACGGGGT TTCATCGTGT TGGCCAGGCT 
GGTCTCCAAC TCTTGACCTC AAGTGATCCA CCTGCCTTGG ACTCCCAAAG TGCTGGGATT 
ACAGGTGCAG ATGCTAATTT CAGTGTCTGG ATTAAAAGGT GTCAAGAAGC TCAGAATGGC 
TCAGAATCTG AGGTGTGGAC TCATCAGTCA AAAATCAAGT ATGACTGGTA TCAAACAGAA 
TCTCAAGTAG TCATTACACT TATGATCAAG AATGTTCAGA AGAATGATGT AAATGTGGAA 
TTTTCAGAAA AAGAGTTGTC TGCTTTGGTT AAACTTCCTT CTGGAGAGGA TTACAATTTG 
AAACTGGAAC TTCTTCATCC TATAATACCA GAACAGAGCA CGTTTAAAGT ACTTTCAACA 
AAGATTGAAA TTAAACTGAA AAAGCCAGAG GCTGTGAGAT GGGAAAAGCT AGAGGGGCAA 
GGAGATGTGC CTACGCCAAA ACAATTCGTA GCAGATGTAA AGAACCTATA TCCATCATCA 
TCTCCTTATA CAAGAAATTG GGATAAATTG GTTGGTGAGA TCAAAGAAGA AGAAAAGAAT 
GAAAAGTTGG AGGGAGATGC AGCTTTAAAC AGATTATTTC AGCAGATCTA TTCAGATGGT 
TCTGATGAAG TGAAACGTGC CATGAACAAA TCCTTTATGG AGTCGGGTGG TACAGTTTTG 
AGTACCAACT GGTCTGATGT AGGTAAAAGG AAAGTTGAAA TCAATCCTCC TGATGATATG 
GAATGGAAAA AGTACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19340
Clone ID Related Accession (Same CDS sequence) NM_001130912.1 , NM_001130912.3 , NM_001130912.2
Accession Version NM_001130912.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1098bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-24
Organism Homo sapiens(human)
Product protein SGT1 homolog isoform B
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN345940.1, AY321358.1, BC000911.1 and AK292128.1. This sequence is a reference standard in the RefSeqGene project. On Mar 9, 2016 this sequence version replaced NM_001130912.1. Transcript Variant: This variant (1, also known as B or SGT1.2) encodes the longest isoform (B). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.31332.1, SRR1803615.3090.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCGGCGG CTGCAGCAGG AACTGCAACA TCCCAGAGGT TTTTCCAGAG CTTCTCGGAT 
GCCCTAATCG ACGAGGACCC CCAGGCGGCG TTAGAGGAGC TGACTAAGGC TTTGGAACAG 
AAACCAGATG ATGCACAGTA TTATTGTCAA AGAGCTTATT GTCACATTCT TCTTGGGAAT 
TACTGTGTTG CTGTTGCTGA TGCAAAGAAG TCTCTAGAAC TCAATCCAAA TAATTCCACT 
GCTATGCTGA GAAAAGGAAT ATGTGAATAC CATGAAAAAA ACTATGCTGC TGCCCTAGAA 
ACTTTTACAG AAGGACAAAA ATTAGATATA GAGACGGGGT TTCATCGTGT TGGCCAGGCT 
GGTCTCCAAC TCTTGACCTC AAGTGATCCA CCTGCCTTGG ACTCCCAAAG TGCTGGGATT 
ACAGGTGCAG ATGCTAATTT CAGTGTCTGG ATTAAAAGGT GTCAAGAAGC TCAGAATGGC 
TCAGAATCTG AGGTGTGGAC TCATCAGTCA AAAATCAAGT ATGACTGGTA TCAAACAGAA 
TCTCAAGTAG TCATTACACT TATGATCAAG AATGTTCAGA AGAATGATGT AAATGTGGAA 
TTTTCAGAAA AAGAGTTGTC TGCTTTGGTT AAACTTCCTT CTGGAGAGGA TTACAATTTG 
AAACTGGAAC TTCTTCATCC TATAATACCA GAACAGAGCA CGTTTAAAGT ACTTTCAACA 
AAGATTGAAA TTAAACTGAA AAAGCCAGAG GCTGTGAGAT GGGAAAAGCT AGAGGGGCAA 
GGAGATGTGC CTACGCCAAA ACAATTCGTA GCAGATGTAA AGAACCTATA TCCATCATCA 
TCTCCTTATA CAAGAAATTG GGATAAATTG GTTGGTGAGA TCAAAGAAGA AGAAAAGAAT 
GAAAAGTTGG AGGGAGATGC AGCTTTAAAC AGATTATTTC AGCAGATCTA TTCAGATGGT 
TCTGATGAAG TGAAACGTGC CATGAACAAA TCCTTTATGG AGTCGGGTGG TACAGTTTTG 
AGTACCAACT GGTCTGATGT AGGTAAAAGG AAAGTTGAAA TCAATCCTCC TGATGATATG 
GAATGGAAAA AGTACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001124384.1
CDS231..1328
Translation

Target ORF information:

RefSeq Version NM_001130912.2
Organism Homo sapiens(human)
Definition Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001130912.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCGGCGG CTGCAGCAGG AACTGCAACA TCCCAGAGGT TTTTCCAGAG CTTCTCGGAT 
GCCCTAATCG ACGAGGACCC CCAGGCGGCG TTAGAGGAGC TGACTAAGGC TTTGGAACAG 
AAACCAGATG ATGCACAGTA TTATTGTCAA AGAGCTTATT GTCACATTCT TCTTGGGAAT 
TACTGTGTTG CTGTTGCTGA TGCAAAGAAG TCTCTAGAAC TCAATCCAAA TAATTCCACT 
GCTATGCTGA GAAAAGGAAT ATGTGAATAC CATGAAAAAA ACTATGCTGC TGCCCTAGAA 
ACTTTTACAG AAGGACAAAA ATTAGATATA GAGACGGGGT TTCATCGTGT TGGCCAGGCT 
GGTCTCCAAC TCTTGACCTC AAGTGATCCA CCTGCCTTGG ACTCCCAAAG TGCTGGGATT 
ACAGGTGCAG ATGCTAATTT CAGTGTCTGG ATTAAAAGGT GTCAAGAAGC TCAGAATGGC 
TCAGAATCTG AGGTGTGGAC TCATCAGTCA AAAATCAAGT ATGACTGGTA TCAAACAGAA 
TCTCAAGTAG TCATTACACT TATGATCAAG AATGTTCAGA AGAATGATGT AAATGTGGAA 
TTTTCAGAAA AAGAGTTGTC TGCTTTGGTT AAACTTCCTT CTGGAGAGGA TTACAATTTG 
AAACTGGAAC TTCTTCATCC TATAATACCA GAACAGAGCA CGTTTAAAGT ACTTTCAACA 
AAGATTGAAA TTAAACTGAA AAAGCCAGAG GCTGTGAGAT GGGAAAAGCT AGAGGGGCAA 
GGAGATGTGC CTACGCCAAA ACAATTCGTA GCAGATGTAA AGAACCTATA TCCATCATCA 
TCTCCTTATA CAAGAAATTG GGATAAATTG GTTGGTGAGA TCAAAGAAGA AGAAAAGAAT 
GAAAAGTTGG AGGGAGATGC AGCTTTAAAC AGATTATTTC AGCAGATCTA TTCAGATGGT 
TCTGATGAAG TGAAACGTGC CATGAACAAA TCCTTTATGG AGTCGGGTGG TACAGTTTTG 
AGTACCAACT GGTCTGATGT AGGTAAAAGG AAAGTTGAAA TCAATCCTCC TGATGATATG 
GAATGGAAAA AGTACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu64514
Clone ID Related Accession (Same CDS sequence) XM_011534893.2 , XM_011534893.3
Accession Version XM_011534893.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 759bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product protein SGT1 homolog isoform X5
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534893.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGCTGAGAA AAGGAATATG TGAATACCAT GAAAAAAACT ATGCTGCTGC CCTAGAAACT 
TTTACAGAAG GACAAAAATT AGATAGTGCA GATGCTAATT TCAGTGTCTG GATTAAAAGG 
TGTCAAGAAG CTCAGAATGG CTCAGAATCT GAGGTGTGGA CTCATCAGTC AAAAATCAAG 
TATGACTGGT ATCAAACAGA ATCTCAAGTA GTCATTACAC TTATGATCAA GAATGTTCAG 
AAGAATGATG TAAATGTGGA ATTTTCAGAA AAAGAGTTGT CTGCTTTGGT TAAACTTCCT 
TCTGGAGAGG ATTACAATTT GAAACTGGAA CTTCTTCATC CTATAATACC AGAACAGAGC 
ACGTTTAAAG TACTTTCAAC AAAGATTGAA ATTAAACTGA AAAAGCCAGA GGCTGTGAGA 
TGGGAAAAGC TAGAGGGGCA AGGAGATGTG CCTACGCCAA AACAATTCGT AGCAGATGTA 
AAGAACCTAT ATCCATCATC ATCTCCTTAT ACAAGAAATT GGGATAAATT GGTTGGTGAG 
ATCAAAGAAG AAGAAAAGAA TGAAAAGTTG GAGGGAGATG CAGCTTTAAA CAGATTATTT 
CAGCAGATCT ATTCAGATGG TTCTGATGAA GTGAAACGTG CCATGAACAA ATCCTTTATG 
GAGTCGGGTG GTACAGTTTT GAGTACCAAC TGGTCTGATG TAGGTAAAAG GAAAGTTGAA 
ATCAATCCTC CTGATGATAT GGAATGGAAA AAGTACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011533195.1
CDS383..1141
Translation

Target ORF information:

RefSeq Version XM_011534893.2
Organism Homo sapiens(human)
Definition Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011534893.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGCTGAGAA AAGGAATATG TGAATACCAT GAAAAAAACT ATGCTGCTGC CCTAGAAACT 
TTTACAGAAG GACAAAAATT AGATAGTGCA GATGCTAATT TCAGTGTCTG GATTAAAAGG 
TGTCAAGAAG CTCAGAATGG CTCAGAATCT GAGGTGTGGA CTCATCAGTC AAAAATCAAG 
TATGACTGGT ATCAAACAGA ATCTCAAGTA GTCATTACAC TTATGATCAA GAATGTTCAG 
AAGAATGATG TAAATGTGGA ATTTTCAGAA AAAGAGTTGT CTGCTTTGGT TAAACTTCCT 
TCTGGAGAGG ATTACAATTT GAAACTGGAA CTTCTTCATC CTATAATACC AGAACAGAGC 
ACGTTTAAAG TACTTTCAAC AAAGATTGAA ATTAAACTGA AAAAGCCAGA GGCTGTGAGA 
TGGGAAAAGC TAGAGGGGCA AGGAGATGTG CCTACGCCAA AACAATTCGT AGCAGATGTA 
AAGAACCTAT ATCCATCATC ATCTCCTTAT ACAAGAAATT GGGATAAATT GGTTGGTGAG 
ATCAAAGAAG AAGAAAAGAA TGAAAAGTTG GAGGGAGATG CAGCTTTAAA CAGATTATTT 
CAGCAGATCT ATTCAGATGG TTCTGATGAA GTGAAACGTG CCATGAACAA ATCCTTTATG 
GAGTCGGGTG GTACAGTTTT GAGTACCAAC TGGTCTGATG TAGGTAAAAG GAAAGTTGAA 
ATCAATCCTC CTGATGATAT GGAATGGAAA AAGTACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu64514
Clone ID Related Accession (Same CDS sequence) XM_011534893.2 , XM_011534893.3
Accession Version XM_011534893.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 759bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product protein SGT1 homolog isoform X5
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011534893.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGCTGAGAA AAGGAATATG TGAATACCAT GAAAAAAACT ATGCTGCTGC CCTAGAAACT 
TTTACAGAAG GACAAAAATT AGATAGTGCA GATGCTAATT TCAGTGTCTG GATTAAAAGG 
TGTCAAGAAG CTCAGAATGG CTCAGAATCT GAGGTGTGGA CTCATCAGTC AAAAATCAAG 
TATGACTGGT ATCAAACAGA ATCTCAAGTA GTCATTACAC TTATGATCAA GAATGTTCAG 
AAGAATGATG TAAATGTGGA ATTTTCAGAA AAAGAGTTGT CTGCTTTGGT TAAACTTCCT 
TCTGGAGAGG ATTACAATTT GAAACTGGAA CTTCTTCATC CTATAATACC AGAACAGAGC 
ACGTTTAAAG TACTTTCAAC AAAGATTGAA ATTAAACTGA AAAAGCCAGA GGCTGTGAGA 
TGGGAAAAGC TAGAGGGGCA AGGAGATGTG CCTACGCCAA AACAATTCGT AGCAGATGTA 
AAGAACCTAT ATCCATCATC ATCTCCTTAT ACAAGAAATT GGGATAAATT GGTTGGTGAG 
ATCAAAGAAG AAGAAAAGAA TGAAAAGTTG GAGGGAGATG CAGCTTTAAA CAGATTATTT 
CAGCAGATCT ATTCAGATGG TTCTGATGAA GTGAAACGTG CCATGAACAA ATCCTTTATG 
GAGTCGGGTG GTACAGTTTT GAGTACCAAC TGGTCTGATG TAGGTAAAAG GAAAGTTGAA 
ATCAATCCTC CTGATGATAT GGAATGGAAA AAGTACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011533195.1
CDS565..1323
Translation

Target ORF information:

RefSeq Version XM_011534893.3
Organism Homo sapiens(human)
Definition Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011534893.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGCTGAGAA AAGGAATATG TGAATACCAT GAAAAAAACT ATGCTGCTGC CCTAGAAACT 
TTTACAGAAG GACAAAAATT AGATAGTGCA GATGCTAATT TCAGTGTCTG GATTAAAAGG 
TGTCAAGAAG CTCAGAATGG CTCAGAATCT GAGGTGTGGA CTCATCAGTC AAAAATCAAG 
TATGACTGGT ATCAAACAGA ATCTCAAGTA GTCATTACAC TTATGATCAA GAATGTTCAG 
AAGAATGATG TAAATGTGGA ATTTTCAGAA AAAGAGTTGT CTGCTTTGGT TAAACTTCCT 
TCTGGAGAGG ATTACAATTT GAAACTGGAA CTTCTTCATC CTATAATACC AGAACAGAGC 
ACGTTTAAAG TACTTTCAAC AAAGATTGAA ATTAAACTGA AAAAGCCAGA GGCTGTGAGA 
TGGGAAAAGC TAGAGGGGCA AGGAGATGTG CCTACGCCAA AACAATTCGT AGCAGATGTA 
AAGAACCTAT ATCCATCATC ATCTCCTTAT ACAAGAAATT GGGATAAATT GGTTGGTGAG 
ATCAAAGAAG AAGAAAAGAA TGAAAAGTTG GAGGGAGATG CAGCTTTAAA CAGATTATTT 
CAGCAGATCT ATTCAGATGG TTCTGATGAA GTGAAACGTG CCATGAACAA ATCCTTTATG 
GAGTCGGGTG GTACAGTTTT GAGTACCAAC TGGTCTGATG TAGGTAAAAG GAAAGTTGAA 
ATCAATCCTC CTGATGATAT GGAATGGAAA AAGTACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu64513
Clone ID Related Accession (Same CDS sequence) NM_001320831.1 , NM_001320831.2
Accession Version NM_001320831.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 834bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-06-02
Organism Homo sapiens(human)
Product protein SGT1 homolog isoform C
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN345940.1, AK302363.1, BC000911.1 and AK292128.1. On Mar 9, 2016 this sequence version replaced XM_011534892.1. Transcript Variant: This variant (3) lacks an alternate exon and contains an alternate exon in the 5' region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (C) has a distinct N-terminus and is shorter than isoform B. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302363.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTTAAGCC AAAAGGAAGT TGCTGTTGCT GATGCAAAGA AGTCTCTAGA ACTCAATCCA 
AATAATTCCA CTGCTATGCT GAGAAAAGGA ATATGTGAAT ACCATGAAAA AAACTATGCT 
GCTGCCCTAG AAACTTTTAC AGAAGGACAA AAATTAGATA GTGCAGATGC TAATTTCAGT 
GTCTGGATTA AAAGGTGTCA AGAAGCTCAG AATGGCTCAG AATCTGAGGT GTGGACTCAT 
CAGTCAAAAA TCAAGTATGA CTGGTATCAA ACAGAATCTC AAGTAGTCAT TACACTTATG 
ATCAAGAATG TTCAGAAGAA TGATGTAAAT GTGGAATTTT CAGAAAAAGA GTTGTCTGCT 
TTGGTTAAAC TTCCTTCTGG AGAGGATTAC AATTTGAAAC TGGAACTTCT TCATCCTATA 
ATACCAGAAC AGAGCACGTT TAAAGTACTT TCAACAAAGA TTGAAATTAA ACTGAAAAAG 
CCAGAGGCTG TGAGATGGGA AAAGCTAGAG GGGCAAGGAG ATGTGCCTAC GCCAAAACAA 
TTCGTAGCAG ATGTAAAGAA CCTATATCCA TCATCATCTC CTTATACAAG AAATTGGGAT 
AAATTGGTTG GTGAGATCAA AGAAGAAGAA AAGAATGAAA AGTTGGAGGG AGATGCAGCT 
TTAAACAGAT TATTTCAGCA GATCTATTCA GATGGTTCTG ATGAAGTGAA ACGTGCCATG 
AACAAATCCT TTATGGAGTC GGGTGGTACA GTTTTGAGTA CCAACTGGTC TGATGTAGGT 
AAAAGGAAAG TTGAAATCAA TCCTCCTGAT GATATGGAAT GGAAAAAGTA CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001307760.1
CDS527..1360
Translation

Target ORF information:

RefSeq Version NM_001320831.1
Organism Homo sapiens(human)
Definition Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001320831.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTTAAGCC AAAAGGAAGT TGCTGTTGCT GATGCAAAGA AGTCTCTAGA ACTCAATCCA 
AATAATTCCA CTGCTATGCT GAGAAAAGGA ATATGTGAAT ACCATGAAAA AAACTATGCT 
GCTGCCCTAG AAACTTTTAC AGAAGGACAA AAATTAGATA GTGCAGATGC TAATTTCAGT 
GTCTGGATTA AAAGGTGTCA AGAAGCTCAG AATGGCTCAG AATCTGAGGT GTGGACTCAT 
CAGTCAAAAA TCAAGTATGA CTGGTATCAA ACAGAATCTC AAGTAGTCAT TACACTTATG 
ATCAAGAATG TTCAGAAGAA TGATGTAAAT GTGGAATTTT CAGAAAAAGA GTTGTCTGCT 
TTGGTTAAAC TTCCTTCTGG AGAGGATTAC AATTTGAAAC TGGAACTTCT TCATCCTATA 
ATACCAGAAC AGAGCACGTT TAAAGTACTT TCAACAAAGA TTGAAATTAA ACTGAAAAAG 
CCAGAGGCTG TGAGATGGGA AAAGCTAGAG GGGCAAGGAG ATGTGCCTAC GCCAAAACAA 
TTCGTAGCAG ATGTAAAGAA CCTATATCCA TCATCATCTC CTTATACAAG AAATTGGGAT 
AAATTGGTTG GTGAGATCAA AGAAGAAGAA AAGAATGAAA AGTTGGAGGG AGATGCAGCT 
TTAAACAGAT TATTTCAGCA GATCTATTCA GATGGTTCTG ATGAAGTGAA ACGTGCCATG 
AACAAATCCT TTATGGAGTC GGGTGGTACA GTTTTGAGTA CCAACTGGTC TGATGTAGGT 
AAAAGGAAAG TTGAAATCAA TCCTCCTGAT GATATGGAAT GGAAAAAGTA CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu64513
Clone ID Related Accession (Same CDS sequence) NM_001320831.1 , NM_001320831.2
Accession Version NM_001320831.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 834bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-26
Organism Homo sapiens(human)
Product protein SGT1 homolog isoform C
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL139089.13. On Jun 2, 2019 this sequence version replaced NM_001320831.1. Transcript Variant: This variant (3) lacks an alternate exon and contains an alternate exon in the 5' region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (C) has a distinct N-terminus and is shorter than isoform B. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.481669.1, AK302363.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTTAAGCC AAAAGGAAGT TGCTGTTGCT GATGCAAAGA AGTCTCTAGA ACTCAATCCA 
AATAATTCCA CTGCTATGCT GAGAAAAGGA ATATGTGAAT ACCATGAAAA AAACTATGCT 
GCTGCCCTAG AAACTTTTAC AGAAGGACAA AAATTAGATA GTGCAGATGC TAATTTCAGT 
GTCTGGATTA AAAGGTGTCA AGAAGCTCAG AATGGCTCAG AATCTGAGGT GTGGACTCAT 
CAGTCAAAAA TCAAGTATGA CTGGTATCAA ACAGAATCTC AAGTAGTCAT TACACTTATG 
ATCAAGAATG TTCAGAAGAA TGATGTAAAT GTGGAATTTT CAGAAAAAGA GTTGTCTGCT 
TTGGTTAAAC TTCCTTCTGG AGAGGATTAC AATTTGAAAC TGGAACTTCT TCATCCTATA 
ATACCAGAAC AGAGCACGTT TAAAGTACTT TCAACAAAGA TTGAAATTAA ACTGAAAAAG 
CCAGAGGCTG TGAGATGGGA AAAGCTAGAG GGGCAAGGAG ATGTGCCTAC GCCAAAACAA 
TTCGTAGCAG ATGTAAAGAA CCTATATCCA TCATCATCTC CTTATACAAG AAATTGGGAT 
AAATTGGTTG GTGAGATCAA AGAAGAAGAA AAGAATGAAA AGTTGGAGGG AGATGCAGCT 
TTAAACAGAT TATTTCAGCA GATCTATTCA GATGGTTCTG ATGAAGTGAA ACGTGCCATG 
AACAAATCCT TTATGGAGTC GGGTGGTACA GTTTTGAGTA CCAACTGGTC TGATGTAGGT 
AAAAGGAAAG TTGAAATCAA TCCTCCTGAT GATATGGAAT GGAAAAAGTA CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001307760.1
CDS382..1215
Translation

Target ORF information:

RefSeq Version NM_001320831.2
Organism Homo sapiens(human)
Definition Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001320831.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTTAAGCC AAAAGGAAGT TGCTGTTGCT GATGCAAAGA AGTCTCTAGA ACTCAATCCA 
AATAATTCCA CTGCTATGCT GAGAAAAGGA ATATGTGAAT ACCATGAAAA AAACTATGCT 
GCTGCCCTAG AAACTTTTAC AGAAGGACAA AAATTAGATA GTGCAGATGC TAATTTCAGT 
GTCTGGATTA AAAGGTGTCA AGAAGCTCAG AATGGCTCAG AATCTGAGGT GTGGACTCAT 
CAGTCAAAAA TCAAGTATGA CTGGTATCAA ACAGAATCTC AAGTAGTCAT TACACTTATG 
ATCAAGAATG TTCAGAAGAA TGATGTAAAT GTGGAATTTT CAGAAAAAGA GTTGTCTGCT 
TTGGTTAAAC TTCCTTCTGG AGAGGATTAC AATTTGAAAC TGGAACTTCT TCATCCTATA 
ATACCAGAAC AGAGCACGTT TAAAGTACTT TCAACAAAGA TTGAAATTAA ACTGAAAAAG 
CCAGAGGCTG TGAGATGGGA AAAGCTAGAG GGGCAAGGAG ATGTGCCTAC GCCAAAACAA 
TTCGTAGCAG ATGTAAAGAA CCTATATCCA TCATCATCTC CTTATACAAG AAATTGGGAT 
AAATTGGTTG GTGAGATCAA AGAAGAAGAA AAGAATGAAA AGTTGGAGGG AGATGCAGCT 
TTAAACAGAT TATTTCAGCA GATCTATTCA GATGGTTCTG ATGAAGTGAA ACGTGCCATG 
AACAAATCCT TTATGGAGTC GGGTGGTACA GTTTTGAGTA CCAACTGGTC TGATGTAGGT 
AAAAGGAAAG TTGAAATCAA TCCTCCTGAT GATATGGAAT GGAAAAAGTA CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu64512
Clone ID Related Accession (Same CDS sequence) XM_011534891.2
Accession Version XM_011534891.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 855bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product protein SGT1 homolog isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534891.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGCTGAGAA AAGGAATATG TGAATACCAT GAAAAAAACT ATGCTGCTGC CCTAGAAACT 
TTTACAGAAG GACAAAAATT AGATATAGAG ACGGGGTTTC ATCGTGTTGG CCAGGCTGGT 
CTCCAACTCT TGACCTCAAG TGATCCACCT GCCTTGGACT CCCAAAGTGC TGGGATTACA 
GGTGCAGATG CTAATTTCAG TGTCTGGATT AAAAGGTGTC AAGAAGCTCA GAATGGCTCA 
GAATCTGAGG TGTGGACTCA TCAGTCAAAA ATCAAGTATG ACTGGTATCA AACAGAATCT 
CAAGTAGTCA TTACACTTAT GATCAAGAAT GTTCAGAAGA ATGATGTAAA TGTGGAATTT 
TCAGAAAAAG AGTTGTCTGC TTTGGTTAAA CTTCCTTCTG GAGAGGATTA CAATTTGAAA 
CTGGAACTTC TTCATCCTAT AATACCAGAA CAGAGCACGT TTAAAGTACT TTCAACAAAG 
ATTGAAATTA AACTGAAAAA GCCAGAGGCT GTGAGATGGG AAAAGCTAGA GGGGCAAGGA 
GATGTGCCTA CGCCAAAACA ATTCGTAGCA GATGTAAAGA ACCTATATCC ATCATCATCT 
CCTTATACAA GAAATTGGGA TAAATTGGTT GGTGAGATCA AAGAAGAAGA AAAGAATGAA 
AAGTTGGAGG GAGATGCAGC TTTAAACAGA TTATTTCAGC AGATCTATTC AGATGGTTCT 
GATGAAGTGA AACGTGCCAT GAACAAATCC TTTATGGAGT CGGGTGGTAC AGTTTTGAGT 
ACCAACTGGT CTGATGTAGG TAAAAGGAAA GTTGAAATCA ATCCTCCTGA TGATATGGAA 
TGGAAAAAGT ACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011533193.1
CDS569..1423
Translation

Target ORF information:

RefSeq Version XM_011534891.2
Organism Homo sapiens(human)
Definition Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011534891.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGCTGAGAA AAGGAATATG TGAATACCAT GAAAAAAACT ATGCTGCTGC CCTAGAAACT 
TTTACAGAAG GACAAAAATT AGATATAGAG ACGGGGTTTC ATCGTGTTGG CCAGGCTGGT 
CTCCAACTCT TGACCTCAAG TGATCCACCT GCCTTGGACT CCCAAAGTGC TGGGATTACA 
GGTGCAGATG CTAATTTCAG TGTCTGGATT AAAAGGTGTC AAGAAGCTCA GAATGGCTCA 
GAATCTGAGG TGTGGACTCA TCAGTCAAAA ATCAAGTATG ACTGGTATCA AACAGAATCT 
CAAGTAGTCA TTACACTTAT GATCAAGAAT GTTCAGAAGA ATGATGTAAA TGTGGAATTT 
TCAGAAAAAG AGTTGTCTGC TTTGGTTAAA CTTCCTTCTG GAGAGGATTA CAATTTGAAA 
CTGGAACTTC TTCATCCTAT AATACCAGAA CAGAGCACGT TTAAAGTACT TTCAACAAAG 
ATTGAAATTA AACTGAAAAA GCCAGAGGCT GTGAGATGGG AAAAGCTAGA GGGGCAAGGA 
GATGTGCCTA CGCCAAAACA ATTCGTAGCA GATGTAAAGA ACCTATATCC ATCATCATCT 
CCTTATACAA GAAATTGGGA TAAATTGGTT GGTGAGATCA AAGAAGAAGA AAAGAATGAA 
AAGTTGGAGG GAGATGCAGC TTTAAACAGA TTATTTCAGC AGATCTATTC AGATGGTTCT 
GATGAAGTGA AACGTGCCAT GAACAAATCC TTTATGGAGT CGGGTGGTAC AGTTTTGAGT 
ACCAACTGGT CTGATGTAGG TAAAAGGAAA GTTGAAATCA ATCCTCCTGA TGATATGGAA 
TGGAAAAAGT ACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu84409
Clone ID Related Accession (Same CDS sequence) XM_017020365.1
Accession Version XM_017020365.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1095bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product protein SGT1 homolog isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCGGCGG CTGCAGCAGG AACTGCAACA TCCCAGAGGT TTTTCCAGAG CTTCTCGGAT 
GCCCTAATCG ACGAGGACCC CCAGGCGGCG TTAGAGGAGC TGACTAAGGC TTTGGAACAG 
AAACCAGATG ATGCACAGTA TTATTGTCAA AGAGCTTATT GTCACATTCT TCTTGGGAAT 
TACTGTGTTG CTGTTGCTGA TGCAAAGAAG TCTCTAGAAC TCAATCCAAA TAATTCCACT 
GCTATGCTGA GAAAAGGAAT ATGTGAATAC CATGAAAAAA ACTATGCTGC TGCCCTAGAA 
ACTTTTACAG AAGGACAAAA ATTAGATAAG ACGGGGTTTC ATCGTGTTGG CCAGGCTGGT 
CTCCAACTCT TGACCTCAAG TGATCCACCT GCCTTGGACT CCCAAAGTGC TGGGATTACA 
GGTGCAGATG CTAATTTCAG TGTCTGGATT AAAAGGTGTC AAGAAGCTCA GAATGGCTCA 
GAATCTGAGG TGTGGACTCA TCAGTCAAAA ATCAAGTATG ACTGGTATCA AACAGAATCT 
CAAGTAGTCA TTACACTTAT GATCAAGAAT GTTCAGAAGA ATGATGTAAA TGTGGAATTT 
TCAGAAAAAG AGTTGTCTGC TTTGGTTAAA CTTCCTTCTG GAGAGGATTA CAATTTGAAA 
CTGGAACTTC TTCATCCTAT AATACCAGAA CAGAGCACGT TTAAAGTACT TTCAACAAAG 
ATTGAAATTA AACTGAAAAA GCCAGAGGCT GTGAGATGGG AAAAGCTAGA GGGGCAAGGA 
GATGTGCCTA CGCCAAAACA ATTCGTAGCA GATGTAAAGA ACCTATATCC ATCATCATCT 
CCTTATACAA GAAATTGGGA TAAATTGGTT GGTGAGATCA AAGAAGAAGA AAAGAATGAA 
AAGTTGGAGG GAGATGCAGC TTTAAACAGA TTATTTCAGC AGATCTATTC AGATGGTTCT 
GATGAAGTGA AACGTGCCAT GAACAAATCC TTTATGGAGT CGGGTGGTAC AGTTTTGAGT 
ACCAACTGGT CTGATGTAGG TAAAAGGAAA GTTGAAATCA ATCCTCCTGA TGATATGGAA 
TGGAAAAAGT ACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016875854.1
CDS219..1313
Translation

Target ORF information:

RefSeq Version XM_017020365.1
Organism Homo sapiens(human)
Definition Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017020365.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCGGCGG CTGCAGCAGG AACTGCAACA TCCCAGAGGT TTTTCCAGAG CTTCTCGGAT 
GCCCTAATCG ACGAGGACCC CCAGGCGGCG TTAGAGGAGC TGACTAAGGC TTTGGAACAG 
AAACCAGATG ATGCACAGTA TTATTGTCAA AGAGCTTATT GTCACATTCT TCTTGGGAAT 
TACTGTGTTG CTGTTGCTGA TGCAAAGAAG TCTCTAGAAC TCAATCCAAA TAATTCCACT 
GCTATGCTGA GAAAAGGAAT ATGTGAATAC CATGAAAAAA ACTATGCTGC TGCCCTAGAA 
ACTTTTACAG AAGGACAAAA ATTAGATAAG ACGGGGTTTC ATCGTGTTGG CCAGGCTGGT 
CTCCAACTCT TGACCTCAAG TGATCCACCT GCCTTGGACT CCCAAAGTGC TGGGATTACA 
GGTGCAGATG CTAATTTCAG TGTCTGGATT AAAAGGTGTC AAGAAGCTCA GAATGGCTCA 
GAATCTGAGG TGTGGACTCA TCAGTCAAAA ATCAAGTATG ACTGGTATCA AACAGAATCT 
CAAGTAGTCA TTACACTTAT GATCAAGAAT GTTCAGAAGA ATGATGTAAA TGTGGAATTT 
TCAGAAAAAG AGTTGTCTGC TTTGGTTAAA CTTCCTTCTG GAGAGGATTA CAATTTGAAA 
CTGGAACTTC TTCATCCTAT AATACCAGAA CAGAGCACGT TTAAAGTACT TTCAACAAAG 
ATTGAAATTA AACTGAAAAA GCCAGAGGCT GTGAGATGGG AAAAGCTAGA GGGGCAAGGA 
GATGTGCCTA CGCCAAAACA ATTCGTAGCA GATGTAAAGA ACCTATATCC ATCATCATCT 
CCTTATACAA GAAATTGGGA TAAATTGGTT GGTGAGATCA AAGAAGAAGA AAAGAATGAA 
AAGTTGGAGG GAGATGCAGC TTTAAACAGA TTATTTCAGC AGATCTATTC AGATGGTTCT 
GATGAAGTGA AACGTGCCAT GAACAAATCC TTTATGGAGT CGGGTGGTAC AGTTTTGAGT 
ACCAACTGGT CTGATGTAGG TAAAAGGAAA GTTGAAATCA ATCCTCCTGA TGATATGGAA 
TGGAAAAAGT ACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu84410
Clone ID Related Accession (Same CDS sequence) XM_017020366.1 , XM_017020367.1 , XM_017020366.2
Accession Version XM_017020366.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 930bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product protein SGT1 homolog isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTTAAGCC AAAAGGAAGT TGCTGTTGCT GATGCAAAGA AGTCTCTAGA ACTCAATCCA 
AATAATTCCA CTGCTATGCT GAGAAAAGGA ATATGTGAAT ACCATGAAAA AAACTATGCT 
GCTGCCCTAG AAACTTTTAC AGAAGGACAA AAATTAGATA TAGAGACGGG GTTTCATCGT 
GTTGGCCAGG CTGGTCTCCA ACTCTTGACC TCAAGTGATC CACCTGCCTT GGACTCCCAA 
AGTGCTGGGA TTACAGGTGC AGATGCTAAT TTCAGTGTCT GGATTAAAAG GTGTCAAGAA 
GCTCAGAATG GCTCAGAATC TGAGGTGTGG ACTCATCAGT CAAAAATCAA GTATGACTGG 
TATCAAACAG AATCTCAAGT AGTCATTACA CTTATGATCA AGAATGTTCA GAAGAATGAT 
GTAAATGTGG AATTTTCAGA AAAAGAGTTG TCTGCTTTGG TTAAACTTCC TTCTGGAGAG 
GATTACAATT TGAAACTGGA ACTTCTTCAT CCTATAATAC CAGAACAGAG CACGTTTAAA 
GTACTTTCAA CAAAGATTGA AATTAAACTG AAAAAGCCAG AGGCTGTGAG ATGGGAAAAG 
CTAGAGGGGC AAGGAGATGT GCCTACGCCA AAACAATTCG TAGCAGATGT AAAGAACCTA 
TATCCATCAT CATCTCCTTA TACAAGAAAT TGGGATAAAT TGGTTGGTGA GATCAAAGAA 
GAAGAAAAGA ATGAAAAGTT GGAGGGAGAT GCAGCTTTAA ACAGATTATT TCAGCAGATC 
TATTCAGATG GTTCTGATGA AGTGAAACGT GCCATGAACA AATCCTTTAT GGAGTCGGGT 
GGTACAGTTT TGAGTACCAA CTGGTCTGAT GTAGGTAAAA GGAAAGTTGA AATCAATCCT 
CCTGATGATA TGGAATGGAA AAAGTACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016875855.1
CDS259..1188
Translation

Target ORF information:

RefSeq Version XM_017020366.1
Organism Homo sapiens(human)
Definition Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017020366.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTTAAGCC AAAAGGAAGT TGCTGTTGCT GATGCAAAGA AGTCTCTAGA ACTCAATCCA 
AATAATTCCA CTGCTATGCT GAGAAAAGGA ATATGTGAAT ACCATGAAAA AAACTATGCT 
GCTGCCCTAG AAACTTTTAC AGAAGGACAA AAATTAGATA TAGAGACGGG GTTTCATCGT 
GTTGGCCAGG CTGGTCTCCA ACTCTTGACC TCAAGTGATC CACCTGCCTT GGACTCCCAA 
AGTGCTGGGA TTACAGGTGC AGATGCTAAT TTCAGTGTCT GGATTAAAAG GTGTCAAGAA 
GCTCAGAATG GCTCAGAATC TGAGGTGTGG ACTCATCAGT CAAAAATCAA GTATGACTGG 
TATCAAACAG AATCTCAAGT AGTCATTACA CTTATGATCA AGAATGTTCA GAAGAATGAT 
GTAAATGTGG AATTTTCAGA AAAAGAGTTG TCTGCTTTGG TTAAACTTCC TTCTGGAGAG 
GATTACAATT TGAAACTGGA ACTTCTTCAT CCTATAATAC CAGAACAGAG CACGTTTAAA 
GTACTTTCAA CAAAGATTGA AATTAAACTG AAAAAGCCAG AGGCTGTGAG ATGGGAAAAG 
CTAGAGGGGC AAGGAGATGT GCCTACGCCA AAACAATTCG TAGCAGATGT AAAGAACCTA 
TATCCATCAT CATCTCCTTA TACAAGAAAT TGGGATAAAT TGGTTGGTGA GATCAAAGAA 
GAAGAAAAGA ATGAAAAGTT GGAGGGAGAT GCAGCTTTAA ACAGATTATT TCAGCAGATC 
TATTCAGATG GTTCTGATGA AGTGAAACGT GCCATGAACA AATCCTTTAT GGAGTCGGGT 
GGTACAGTTT TGAGTACCAA CTGGTCTGAT GTAGGTAAAA GGAAAGTTGA AATCAATCCT 
CCTGATGATA TGGAATGGAA AAAGTACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu84410
Clone ID Related Accession (Same CDS sequence) XM_017020366.1 , XM_017020367.1 , XM_017020366.2
Accession Version XM_017020367.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 930bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product protein SGT1 homolog isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTTAAGCC AAAAGGAAGT TGCTGTTGCT GATGCAAAGA AGTCTCTAGA ACTCAATCCA 
AATAATTCCA CTGCTATGCT GAGAAAAGGA ATATGTGAAT ACCATGAAAA AAACTATGCT 
GCTGCCCTAG AAACTTTTAC AGAAGGACAA AAATTAGATA TAGAGACGGG GTTTCATCGT 
GTTGGCCAGG CTGGTCTCCA ACTCTTGACC TCAAGTGATC CACCTGCCTT GGACTCCCAA 
AGTGCTGGGA TTACAGGTGC AGATGCTAAT TTCAGTGTCT GGATTAAAAG GTGTCAAGAA 
GCTCAGAATG GCTCAGAATC TGAGGTGTGG ACTCATCAGT CAAAAATCAA GTATGACTGG 
TATCAAACAG AATCTCAAGT AGTCATTACA CTTATGATCA AGAATGTTCA GAAGAATGAT 
GTAAATGTGG AATTTTCAGA AAAAGAGTTG TCTGCTTTGG TTAAACTTCC TTCTGGAGAG 
GATTACAATT TGAAACTGGA ACTTCTTCAT CCTATAATAC CAGAACAGAG CACGTTTAAA 
GTACTTTCAA CAAAGATTGA AATTAAACTG AAAAAGCCAG AGGCTGTGAG ATGGGAAAAG 
CTAGAGGGGC AAGGAGATGT GCCTACGCCA AAACAATTCG TAGCAGATGT AAAGAACCTA 
TATCCATCAT CATCTCCTTA TACAAGAAAT TGGGATAAAT TGGTTGGTGA GATCAAAGAA 
GAAGAAAAGA ATGAAAAGTT GGAGGGAGAT GCAGCTTTAA ACAGATTATT TCAGCAGATC 
TATTCAGATG GTTCTGATGA AGTGAAACGT GCCATGAACA AATCCTTTAT GGAGTCGGGT 
GGTACAGTTT TGAGTACCAA CTGGTCTGAT GTAGGTAAAA GGAAAGTTGA AATCAATCCT 
CCTGATGATA TGGAATGGAA AAAGTACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016875856.1
CDS293..1222
Translation

Target ORF information:

RefSeq Version XM_017020367.1
Organism Homo sapiens(human)
Definition Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017020367.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTTAAGCC AAAAGGAAGT TGCTGTTGCT GATGCAAAGA AGTCTCTAGA ACTCAATCCA 
AATAATTCCA CTGCTATGCT GAGAAAAGGA ATATGTGAAT ACCATGAAAA AAACTATGCT 
GCTGCCCTAG AAACTTTTAC AGAAGGACAA AAATTAGATA TAGAGACGGG GTTTCATCGT 
GTTGGCCAGG CTGGTCTCCA ACTCTTGACC TCAAGTGATC CACCTGCCTT GGACTCCCAA 
AGTGCTGGGA TTACAGGTGC AGATGCTAAT TTCAGTGTCT GGATTAAAAG GTGTCAAGAA 
GCTCAGAATG GCTCAGAATC TGAGGTGTGG ACTCATCAGT CAAAAATCAA GTATGACTGG 
TATCAAACAG AATCTCAAGT AGTCATTACA CTTATGATCA AGAATGTTCA GAAGAATGAT 
GTAAATGTGG AATTTTCAGA AAAAGAGTTG TCTGCTTTGG TTAAACTTCC TTCTGGAGAG 
GATTACAATT TGAAACTGGA ACTTCTTCAT CCTATAATAC CAGAACAGAG CACGTTTAAA 
GTACTTTCAA CAAAGATTGA AATTAAACTG AAAAAGCCAG AGGCTGTGAG ATGGGAAAAG 
CTAGAGGGGC AAGGAGATGT GCCTACGCCA AAACAATTCG TAGCAGATGT AAAGAACCTA 
TATCCATCAT CATCTCCTTA TACAAGAAAT TGGGATAAAT TGGTTGGTGA GATCAAAGAA 
GAAGAAAAGA ATGAAAAGTT GGAGGGAGAT GCAGCTTTAA ACAGATTATT TCAGCAGATC 
TATTCAGATG GTTCTGATGA AGTGAAACGT GCCATGAACA AATCCTTTAT GGAGTCGGGT 
GGTACAGTTT TGAGTACCAA CTGGTCTGAT GTAGGTAAAA GGAAAGTTGA AATCAATCCT 
CCTGATGATA TGGAATGGAA AAAGTACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu84410
Clone ID Related Accession (Same CDS sequence) XM_017020366.1 , XM_017020367.1 , XM_017020366.2
Accession Version XM_017020366.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 930bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product protein SGT1 homolog isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017020366.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTTAAGCC AAAAGGAAGT TGCTGTTGCT GATGCAAAGA AGTCTCTAGA ACTCAATCCA 
AATAATTCCA CTGCTATGCT GAGAAAAGGA ATATGTGAAT ACCATGAAAA AAACTATGCT 
GCTGCCCTAG AAACTTTTAC AGAAGGACAA AAATTAGATA TAGAGACGGG GTTTCATCGT 
GTTGGCCAGG CTGGTCTCCA ACTCTTGACC TCAAGTGATC CACCTGCCTT GGACTCCCAA 
AGTGCTGGGA TTACAGGTGC AGATGCTAAT TTCAGTGTCT GGATTAAAAG GTGTCAAGAA 
GCTCAGAATG GCTCAGAATC TGAGGTGTGG ACTCATCAGT CAAAAATCAA GTATGACTGG 
TATCAAACAG AATCTCAAGT AGTCATTACA CTTATGATCA AGAATGTTCA GAAGAATGAT 
GTAAATGTGG AATTTTCAGA AAAAGAGTTG TCTGCTTTGG TTAAACTTCC TTCTGGAGAG 
GATTACAATT TGAAACTGGA ACTTCTTCAT CCTATAATAC CAGAACAGAG CACGTTTAAA 
GTACTTTCAA CAAAGATTGA AATTAAACTG AAAAAGCCAG AGGCTGTGAG ATGGGAAAAG 
CTAGAGGGGC AAGGAGATGT GCCTACGCCA AAACAATTCG TAGCAGATGT AAAGAACCTA 
TATCCATCAT CATCTCCTTA TACAAGAAAT TGGGATAAAT TGGTTGGTGA GATCAAAGAA 
GAAGAAAAGA ATGAAAAGTT GGAGGGAGAT GCAGCTTTAA ACAGATTATT TCAGCAGATC 
TATTCAGATG GTTCTGATGA AGTGAAACGT GCCATGAACA AATCCTTTAT GGAGTCGGGT 
GGTACAGTTT TGAGTACCAA CTGGTCTGAT GTAGGTAAAA GGAAAGTTGA AATCAATCCT 
CCTGATGATA TGGAATGGAA AAAGTACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016875855.1
CDS374..1303
Translation

Target ORF information:

RefSeq Version XM_017020366.2
Organism Homo sapiens(human)
Definition Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017020366.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTTAAGCC AAAAGGAAGT TGCTGTTGCT GATGCAAAGA AGTCTCTAGA ACTCAATCCA 
AATAATTCCA CTGCTATGCT GAGAAAAGGA ATATGTGAAT ACCATGAAAA AAACTATGCT 
GCTGCCCTAG AAACTTTTAC AGAAGGACAA AAATTAGATA TAGAGACGGG GTTTCATCGT 
GTTGGCCAGG CTGGTCTCCA ACTCTTGACC TCAAGTGATC CACCTGCCTT GGACTCCCAA 
AGTGCTGGGA TTACAGGTGC AGATGCTAAT TTCAGTGTCT GGATTAAAAG GTGTCAAGAA 
GCTCAGAATG GCTCAGAATC TGAGGTGTGG ACTCATCAGT CAAAAATCAA GTATGACTGG 
TATCAAACAG AATCTCAAGT AGTCATTACA CTTATGATCA AGAATGTTCA GAAGAATGAT 
GTAAATGTGG AATTTTCAGA AAAAGAGTTG TCTGCTTTGG TTAAACTTCC TTCTGGAGAG 
GATTACAATT TGAAACTGGA ACTTCTTCAT CCTATAATAC CAGAACAGAG CACGTTTAAA 
GTACTTTCAA CAAAGATTGA AATTAAACTG AAAAAGCCAG AGGCTGTGAG ATGGGAAAAG 
CTAGAGGGGC AAGGAGATGT GCCTACGCCA AAACAATTCG TAGCAGATGT AAAGAACCTA 
TATCCATCAT CATCTCCTTA TACAAGAAAT TGGGATAAAT TGGTTGGTGA GATCAAAGAA 
GAAGAAAAGA ATGAAAAGTT GGAGGGAGAT GCAGCTTTAA ACAGATTATT TCAGCAGATC 
TATTCAGATG GTTCTGATGA AGTGAAACGT GCCATGAACA AATCCTTTAT GGAGTCGGGT 
GGTACAGTTT TGAGTACCAA CTGGTCTGAT GTAGGTAAAA GGAAAGTTGA AATCAATCCT 
CCTGATGATA TGGAATGGAA AAAGTACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu84411
Clone ID Related Accession (Same CDS sequence) XM_017020368.1
Accession Version XM_017020368.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 852bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product protein SGT1 homolog isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGCTGAGAA AAGGAATATG TGAATACCAT GAAAAAAACT ATGCTGCTGC CCTAGAAACT 
TTTACAGAAG GACAAAAATT AGATAAGACG GGGTTTCATC GTGTTGGCCA GGCTGGTCTC 
CAACTCTTGA CCTCAAGTGA TCCACCTGCC TTGGACTCCC AAAGTGCTGG GATTACAGGT 
GCAGATGCTA ATTTCAGTGT CTGGATTAAA AGGTGTCAAG AAGCTCAGAA TGGCTCAGAA 
TCTGAGGTGT GGACTCATCA GTCAAAAATC AAGTATGACT GGTATCAAAC AGAATCTCAA 
GTAGTCATTA CACTTATGAT CAAGAATGTT CAGAAGAATG ATGTAAATGT GGAATTTTCA 
GAAAAAGAGT TGTCTGCTTT GGTTAAACTT CCTTCTGGAG AGGATTACAA TTTGAAACTG 
GAACTTCTTC ATCCTATAAT ACCAGAACAG AGCACGTTTA AAGTACTTTC AACAAAGATT 
GAAATTAAAC TGAAAAAGCC AGAGGCTGTG AGATGGGAAA AGCTAGAGGG GCAAGGAGAT 
GTGCCTACGC CAAAACAATT CGTAGCAGAT GTAAAGAACC TATATCCATC ATCATCTCCT 
TATACAAGAA ATTGGGATAA ATTGGTTGGT GAGATCAAAG AAGAAGAAAA GAATGAAAAG 
TTGGAGGGAG ATGCAGCTTT AAACAGATTA TTTCAGCAGA TCTATTCAGA TGGTTCTGAT 
GAAGTGAAAC GTGCCATGAA CAAATCCTTT ATGGAGTCGG GTGGTACAGT TTTGAGTACC 
AACTGGTCTG ATGTAGGTAA AAGGAAAGTT GAAATCAATC CTCCTGATGA TATGGAATGG 
AAAAAGTACT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016875857.1
CDS383..1234
Translation

Target ORF information:

RefSeq Version XM_017020368.1
Organism Homo sapiens(human)
Definition Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017020368.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGCTGAGAA AAGGAATATG TGAATACCAT GAAAAAAACT ATGCTGCTGC CCTAGAAACT 
TTTACAGAAG GACAAAAATT AGATAAGACG GGGTTTCATC GTGTTGGCCA GGCTGGTCTC 
CAACTCTTGA CCTCAAGTGA TCCACCTGCC TTGGACTCCC AAAGTGCTGG GATTACAGGT 
GCAGATGCTA ATTTCAGTGT CTGGATTAAA AGGTGTCAAG AAGCTCAGAA TGGCTCAGAA 
TCTGAGGTGT GGACTCATCA GTCAAAAATC AAGTATGACT GGTATCAAAC AGAATCTCAA 
GTAGTCATTA CACTTATGAT CAAGAATGTT CAGAAGAATG ATGTAAATGT GGAATTTTCA 
GAAAAAGAGT TGTCTGCTTT GGTTAAACTT CCTTCTGGAG AGGATTACAA TTTGAAACTG 
GAACTTCTTC ATCCTATAAT ACCAGAACAG AGCACGTTTA AAGTACTTTC AACAAAGATT 
GAAATTAAAC TGAAAAAGCC AGAGGCTGTG AGATGGGAAA AGCTAGAGGG GCAAGGAGAT 
GTGCCTACGC CAAAACAATT CGTAGCAGAT GTAAAGAACC TATATCCATC ATCATCTCCT 
TATACAAGAA ATTGGGATAA ATTGGTTGGT GAGATCAAAG AAGAAGAAAA GAATGAAAAG 
TTGGAGGGAG ATGCAGCTTT AAACAGATTA TTTCAGCAGA TCTATTCAGA TGGTTCTGAT 
GAAGTGAAAC GTGCCATGAA CAAATCCTTT ATGGAGTCGG GTGGTACAGT TTTGAGTACC 
AACTGGTCTG ATGTAGGTAA AAGGAAAGTT GAAATCAATC CTCCTGATGA TATGGAATGG 
AAAAAGTACT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

SGT1-HSP90 complex is required for CENP-A deposition at centromeres.
Cell cycle (Georgetown, Tex.)16(18)1683-1694(2017 Sep)
Niikura Y,Kitagawa R,Ogi H,Kitagawa K


book

Interplay between the phosphatase PHLPP1 and E3 ligase RNF41 stimulates proper kinetochore assembly via the outer-kinetochore protein SGT1.
The Journal of biological chemistry292(34)13947-13958(2017 08)
Gangula NR,Maddika S


book

Application of SGT1-Hsp90 chaperone complex for soluble expression of NOD1 LRR domain in E.?coli.
Biochemical and biophysical research communications478(4)1647-52(2016 09)
Hong TJ,Hahn JS


book

Translation elongation factor eEF1A1 is a novel partner of a multifunctional protein Sgt1.
Biochimie119137-45(2015 Dec)
Novosylna O,Jurewicz E,Pydiura N,Goral A,Filipek A,Negrutskii B,El'skaya A