DBF4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Gene Symbol	DBF4
Entrez Gene ID	10926
Full Name	DBF4 zinc finger
Synonyms	ASK,CHIF,DBF4A,ZDBF1
General protein information	Preferred Names DBF4 zinc finger Names protein DBF4 homolog A DBF4 homolog DBF4 zinc finger A DBF4-type zinc finger-containing protein 1 activator of S phase kinase chiffon homolog A zinc finger, DBF-type containing 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q21.12
Disorder MIM:	604281

mRNA and Protein(s)

mRNA	Protein	Name
NM_001318061.1	NP_001304990.1	protein DBF4 homolog A isoform 3
NM_001318060.1	NP_001304989.1	protein DBF4 homolog A isoform 2
NM_001318062.1	NP_001304991.1	protein DBF4 homolog A isoform 4
NM_001318061.2	NP_001304990.1	protein DBF4 homolog A isoform 3
NM_006716.4	NP_006707.1	protein DBF4 homolog A isoform 1
NM_001318060.2	NP_001304989.1	protein DBF4 homolog A isoform 2
NM_001318062.2	NP_001304991.1	protein DBF4 homolog A isoform 4
NM_006716.3	NP_006707.1	protein DBF4 homolog A isoform 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DBF4	NP_001068944.2
Rattus norvegicus (Norway rat)	Dbf4	NP_001178677.1
Rattus norvegicus (Norway rat)	LOC100912278	XP_003749729.1
Pan troglodytes (chimpanzee)	DBF4	XP_528041.3
	DBF4	XP_001104753.2
Gallus gallus (chicken)	DBF4	XP_004939326.1
Mus musculus (house mouse)	Dbf4	NP_001177646.1
Homo sapiens (human)	DBF4	NP_006707.1
Canis lupus familiaris (dog)	DBF4	XP_532451.2

Gene Ontology
Bibliography

Related articles in PubMed

Cdc7-Dbf4-mediated phosphorylation of HSP90-S164 stabilizes HSP90-HCLK2-MRN complex to enhance ATR/ATM signaling that overcomes replication stress in cancer.
Cheng AN, Fan CC, Lo YK, Kuo CL, Wang HC, Lien IH, Lin SY, Chen CH, Jiang SS, Chang IS, Juan HF, Lyu PC, Lee AY
Scientific reports7(1)17024(2017 Dec)

DDK Promotes Tumor Chemoresistance and Survival via Multiple Pathways.
Sasi NK, Bhutkar A, Lanning NJ, MacKeigan JP, Weinreich M
Neoplasia (New York, N.Y.)19(5)439-450(2017 May)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

DDK dependent regulation of TOP2A at centromeres revealed by a chemical genetics approach.
Wu KZ, Wang GN, Fitzgerald J, Quachthithu H, Rainey MD, Cattaneo A, Bachi A, Santocanale C
Nucleic acids research44(18)8786-8798(2016 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we propose that phosphorylation of TOP2A by CDC7/DBF4 in early S-phase prevents its localization and/or activity at centromeres, and inhibition of TOP2A function could be relevant to prevent premature separation of centromeric DNA.
Title: DDK dependent regulation of TOP2A at centromeres revealed by a chemical genetics approach.

Histone H3 lysine 9 (H3K9) acetylation was most prevalent when the Dbf4 transcription level was highest whereas the H3K9me3 level was greatest during and just after replication.
Title: Dynamic regulation of histone H3K9 is linked to the switch between replication and transcription at the Dbf4 origin-promoter locus.

The anti-invasive and cell cycle arrest-inducing effects of nitrogen-containing bisphosphonates are not DBF4 mediated in human breast cancer cells.
Title: The cell cycle arrest and the anti-invasive effects of nitrogen-containing bisphosphonates are not mediated by DBF4 in breast cancer cells.

Dbf4 is direct downstream target of ataxia telangiectasia mutated (ATM) and ataxia telangiectasia and Rad3-related (ATR) protein to regulate intra-S-phase checkpoint.
Title: Dbf4 is direct downstream target of ataxia telangiectasia mutated (ATM) and ataxia telangiectasia and Rad3-related (ATR) protein to regulate intra-S-phase checkpoint.

bipartite interaction between Cdc7 and Dbf4/ASK subunits facilitates ATP binding and substrate recognition by the Cdc7 kinase.
Title: Molecular mechanism of activation of human Cdc7 kinase: bipartite interaction with Dbf4/activator of S phase kinase (ASK) activation subunit stimulates ATP binding and substrate recognition.

The following DBF4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DBF4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01796	NM_006716.4 Latest version!	Homo sapiens DBF4 zinc finger (DBF4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu41348	NM_001318061.2 Latest version!	Homo sapiens DBF4 zinc finger (DBF4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu64528	NM_001318062.2 Latest version!	Homo sapiens DBF4 zinc finger (DBF4), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu101514	NM_001318060.2 Latest version!	Homo sapiens DBF4 zinc finger (DBF4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu41348	NM_001318061.1	Homo sapiens DBF4 zinc finger (DBF4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu101514	NM_001318060.1	Homo sapiens DBF4 zinc finger (DBF4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu64528	NM_001318062.1	Homo sapiens DBF4 zinc finger (DBF4), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu01796	NM_006716.3	Homo sapiens DBF4 zinc finger (DBF4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DBF4 ( NM_006716.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006707.1 Homo sapiens DBF4 zinc finger (DBF4), transcript variant 1, mRNA.

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CloneID	OHu01796
Clone ID Related Accession (Same CDS sequence)	NM_006716.4 , NM_006716.3
Accession Version	NM_006716.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	protein DBF4 homolog A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB028069.1, BC036045.1 and AC005164.2. On Nov 23, 2018 this sequence version replaced NM_006716.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC036045.1, SRR1803614.91073.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000265728.6/ ENSP00000265728.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAACTCCG GAGCCATGAG GATCCACAGT AAAGGACATT TCCAGGGTGG AATCCAAGTC 
AAAAATGAAA AAAACAGACC ATCTCTGAAA TCTCTGAAAA CTGATAACAG GCCAGAAAAA 
TCCAAATGTA AGCCACTTTG GGGAAAAGTA TTTTACCTTG ACTTACCTTC TGTCACCATA 
TCTGAAAAAC TTCAAAAGGA CATTAAGGAT CTGGGAGGGC GAGTTGAAGA ATTTCTCAGC 
AAAGATATCA GTTATCTTAT TTCAAATAAG AAGGAAGCTA AATTTGCACA AACCTTGGGT 
CGAATTTCTC CTGTACCAAG TCCAGAATCT GCATATACTG CAGAAACCAC TTCACCTCAT 
CCCAGCCATG ATGGAAGTTC ATTTAAGTCA CCAGACACAG TGTGTTTAAG CAGAGGAAAA 
TTATTAGTTG AAAAAGCTAT CAAGGACCAT GATTTTATTC CTTCAAATAG TATATTATCA 
AATGCCTTGT CATGGGGAGT AAAAATTCTT CATATTGATG ACATTAGATA CTACATTGAA 
CAAAAGAAAA AAGAGTTGTA TTTACTCAAG AAATCAAGTA CTTCAGTAAG AGATGGGGGC 
AAAAGAGTTG GTAGTGGTGC ACAAAAAACA AGAACAGGAA GACTCAAAAA GCCTTTTGTA 
AAGGTGGAAG ATATGAGCCA ACTTTATAGG CCATTTTATC TTCAGCTGAC CAATATGCCT 
TTTATAAATT ATTCTATTCA GAAGCCCTGC AGTCCATTTG ATGTAGACAA GCCATCTAGT 
ATGCAAAAGC AAACTCAGGT TAAACTAAGA ATCCAAACAG ATGGCGATAA GTATGGTGGA 
ACCTCAATTC AACTCCAGTT GAAAGAGAAG AAGAAAAAAG GATATTGTGA ATGTTGCTTG 
CAGAAATATG AAGATCTAGA AACTCACCTT CTAAGTGAGC AACACAGAAA CTTTGCACAG 
AGTAACCAGT ATCAAGTTGT TGATGATATT GTATCTAAGT TAGTTTTTGA CTTTGTGGAA 
TATGAAAAGG ACACACCTAA AAAGAAAAGA ATAAAATACA GTGTTGGATC CCTTTCTCCT 
GTTTCTGCAA GTGTCCTGAA AAAGACTGAA CAAAAGGAAA AAGTGGAATT GCAACATATT 
TCTCAGAAAG ATTGCCAGGA AGATGATACA ACAGTGAAGG AGCAGAATTT CCTGTATAAA 
GAGACCCAGG AAACTGAAAA AAAGCTCCTG TTTATTTCAG AGCCCATCCC CCACCCTTCA 
AATGAATTGA GAGGGCTTAA TGAGAAAATG AGTAATAAAT GTTCCATGTT AAGTACAGCT 
GAAGATGACA TAAGACAGAA TTTTACACAG CTACCTCTAC ATAAAAACAA ACAGGAATGC 
ATTCTTGACA TTTCCGAACA CACATTAAGT GAAAATGACT TAGAAGAACT AAGGGTAGAT 
CACTATAAAT GTAACATACA GGCATCTGTA CATGTTTCTG ATTTCAGTAC AGATAATAGT 
GGATCTCAAC CAAAACAGAA GTCAGATACT GTGCTTTTTC CAGCAAAGGA TCTCAAGGAA 
AAGGACCTTC ATTCAATATT TACTCATGAT TCTGGTCTGA TAACAATAAA CAGTTCACAA 
GAGCACCTAA CTGTTCAGGC AAAGGCTCCA TTCCATACTC CTCCTGAGGA ACCCAATGAA 
TGTGACTTCA AGAATATGGA TAGTTTACCT TCTGGTAAAA TACATCGAAA AGTGAAAATA 
ATATTAGGAC GAAATAGAAA AGAAAATCTG GAACCAAATG CTGAATTTGA TAAAAGAACT 
GAATTTATTA CACAAGAAGA AAACAGAATT TGTAGTTCAC CGGTACAGTC TTTACTAGAC 
TTGTTTCAGA CTAGTGAAGA GAAATCAGAA TTTTTGGGTT TCACAAGCTA CACAGAAAAG 
AGTGGTATAT GCAATGTTTT AGATATTTGG GAAGAGGAAA ATTCAGATAA TCTGTTAACA 
GCGTTTTTCT CGTCCCCTTC AACTTCTACA TTTACTGGCT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006707.1
CDS	241..2265
Translation	MNSGAMRIHSKGHFQGGIQVKNEKNRPSLKSLKTDNRPEKSKCKPLWGKVFYLDLPSVTISEKLQKDIKDLGGRVEEFLSKDISYLISNKKEAKFAQTLGRISPVPSPESAYTAETTSPHPSHDGSSFKSPDTVCLSRGKLLVEKAIKDHDFIPSNSILSNALSWGVKILHIDDIRYYIEQKKKELYLLKKSSTSVRDGGKRVGSGAQKTRTGRLKKPFVKVEDMSQLYRPFYLQLTNMPFINYSIQKPCSPFDVDKPSSMQKQTQVKLRIQTDGDKYGGTSIQLQLKEKKKKGYCECCLQKYEDLETHLLSEQHRNFAQSNQYQVVDDIVSKLVFDFVEYEKDTPKKKRIKYSVGSLSPVSASVLKKTEQKEKVELQHISQKDCQEDDTTVKEQNFLYKETQETEKKLLFISEPIPHPSNELRGLNEKMSNKCSMLSTAEDDIRQNFTQLPLHKNKQECILDISEHTLSENDLEELRVDHYKCNIQASVHVSDFSTDNSGSQPKQKSDTVLFPAKDLKEKDLHSIFTHDSGLITINSSQEHLTVQAKAPFHTPPEEPNECDFKNMDSLPSGKIHRKVKIILGRNRKENLEPNAEFDKRTEFITQEENRICSSPVQSLLDLFQTSEEKSEFLGFTSYTEKSGICNVLDIWEEENSDNLLTAFFSSPSTSTFTGF

Target ORF information:

RefSeq Version	NM_006716.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DBF4 zinc finger (DBF4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006716.4

ORF Insert Sequence:

ATGAACTCCG GAGCCATGAG GATCCACAGT AAAGGACATT TCCAGGGTGG AATCCAAGTC 
AAAAATGAAA AAAACAGACC ATCTCTGAAA TCTCTGAAAA CTGATAACAG GCCAGAAAAA 
TCCAAATGTA AGCCACTTTG GGGAAAAGTA TTTTACCTTG ACTTACCTTC TGTCACCATA 
TCTGAAAAAC TTCAAAAGGA CATTAAGGAT CTGGGAGGGC GAGTTGAAGA ATTTCTCAGC 
AAAGATATCA GTTATCTTAT TTCAAATAAG AAGGAAGCTA AATTTGCACA AACCTTGGGT 
CGAATTTCTC CTGTACCAAG TCCAGAATCT GCATATACTG CAGAAACCAC TTCACCTCAT 
CCCAGCCATG ATGGAAGTTC ATTTAAGTCA CCAGACACAG TGTGTTTAAG CAGAGGAAAA 
TTATTAGTTG AAAAAGCTAT CAAGGACCAT GATTTTATTC CTTCAAATAG TATATTATCA 
AATGCCTTGT CATGGGGAGT AAAAATTCTT CATATTGATG ACATTAGATA CTACATTGAA 
CAAAAGAAAA AAGAGTTGTA TTTACTCAAG AAATCAAGTA CTTCAGTAAG AGATGGGGGC 
AAAAGAGTTG GTAGTGGTGC ACAAAAAACA AGAACAGGAA GACTCAAAAA GCCTTTTGTA 
AAGGTGGAAG ATATGAGCCA ACTTTATAGG CCATTTTATC TTCAGCTGAC CAATATGCCT 
TTTATAAATT ATTCTATTCA GAAGCCCTGC AGTCCATTTG ATGTAGACAA GCCATCTAGT 
ATGCAAAAGC AAACTCAGGT TAAACTAAGA ATCCAAACAG ATGGCGATAA GTATGGTGGA 
ACCTCAATTC AACTCCAGTT GAAAGAGAAG AAGAAAAAAG GATATTGTGA ATGTTGCTTG 
CAGAAATATG AAGATCTAGA AACTCACCTT CTAAGTGAGC AACACAGAAA CTTTGCACAG 
AGTAACCAGT ATCAAGTTGT TGATGATATT GTATCTAAGT TAGTTTTTGA CTTTGTGGAA 
TATGAAAAGG ACACACCTAA AAAGAAAAGA ATAAAATACA GTGTTGGATC CCTTTCTCCT 
GTTTCTGCAA GTGTCCTGAA AAAGACTGAA CAAAAGGAAA AAGTGGAATT GCAACATATT 
TCTCAGAAAG ATTGCCAGGA AGATGATACA ACAGTGAAGG AGCAGAATTT CCTGTATAAA 
GAGACCCAGG AAACTGAAAA AAAGCTCCTG TTTATTTCAG AGCCCATCCC CCACCCTTCA 
AATGAATTGA GAGGGCTTAA TGAGAAAATG AGTAATAAAT GTTCCATGTT AAGTACAGCT 
GAAGATGACA TAAGACAGAA TTTTACACAG CTACCTCTAC ATAAAAACAA ACAGGAATGC 
ATTCTTGACA TTTCCGAACA CACATTAAGT GAAAATGACT TAGAAGAACT AAGGGTAGAT 
CACTATAAAT GTAACATACA GGCATCTGTA CATGTTTCTG ATTTCAGTAC AGATAATAGT 
GGATCTCAAC CAAAACAGAA GTCAGATACT GTGCTTTTTC CAGCAAAGGA TCTCAAGGAA 
AAGGACCTTC ATTCAATATT TACTCATGAT TCTGGTCTGA TAACAATAAA CAGTTCACAA 
GAGCACCTAA CTGTTCAGGC AAAGGCTCCA TTCCATACTC CTCCTGAGGA ACCCAATGAA 
TGTGACTTCA AGAATATGGA TAGTTTACCT TCTGGTAAAA TACATCGAAA AGTGAAAATA 
ATATTAGGAC GAAATAGAAA AGAAAATCTG GAACCAAATG CTGAATTTGA TAAAAGAACT 
GAATTTATTA CACAAGAAGA AAACAGAATT TGTAGTTCAC CGGTACAGTC TTTACTAGAC 
TTGTTTCAGA CTAGTGAAGA GAAATCAGAA TTTTTGGGTT TCACAAGCTA CACAGAAAAG 
AGTGGTATAT GCAATGTTTT AGATATTTGG GAAGAGGAAA ATTCAGATAA TCTGTTAACA 
GCGTTTTTCT CGTCCCCTTC AACTTCTACA TTTACTGGCT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DBF4 ( NM_006716.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006707.1 Homo sapiens DBF4 zinc finger (DBF4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01796
Clone ID Related Accession (Same CDS sequence)	NM_006716.4 , NM_006716.3
Accession Version	NM_006716.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	protein DBF4 homolog A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB028069.1 and BC036045.1. On Jan 30, 2004 this sequence version replaced NM_006716.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC036045.1, SRR1803614.91073.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTCCG GAGCCATGAG GATCCACAGT AAAGGACATT TCCAGGGTGG AATCCAAGTC 
AAAAATGAAA AAAACAGACC ATCTCTGAAA TCTCTGAAAA CTGATAACAG GCCAGAAAAA 
TCCAAATGTA AGCCACTTTG GGGAAAAGTA TTTTACCTTG ACTTACCTTC TGTCACCATA 
TCTGAAAAAC TTCAAAAGGA CATTAAGGAT CTGGGAGGGC GAGTTGAAGA ATTTCTCAGC 
AAAGATATCA GTTATCTTAT TTCAAATAAG AAGGAAGCTA AATTTGCACA AACCTTGGGT 
CGAATTTCTC CTGTACCAAG TCCAGAATCT GCATATACTG CAGAAACCAC TTCACCTCAT 
CCCAGCCATG ATGGAAGTTC ATTTAAGTCA CCAGACACAG TGTGTTTAAG CAGAGGAAAA 
TTATTAGTTG AAAAAGCTAT CAAGGACCAT GATTTTATTC CTTCAAATAG TATATTATCA 
AATGCCTTGT CATGGGGAGT AAAAATTCTT CATATTGATG ACATTAGATA CTACATTGAA 
CAAAAGAAAA AAGAGTTGTA TTTACTCAAG AAATCAAGTA CTTCAGTAAG AGATGGGGGC 
AAAAGAGTTG GTAGTGGTGC ACAAAAAACA AGAACAGGAA GACTCAAAAA GCCTTTTGTA 
AAGGTGGAAG ATATGAGCCA ACTTTATAGG CCATTTTATC TTCAGCTGAC CAATATGCCT 
TTTATAAATT ATTCTATTCA GAAGCCCTGC AGTCCATTTG ATGTAGACAA GCCATCTAGT 
ATGCAAAAGC AAACTCAGGT TAAACTAAGA ATCCAAACAG ATGGCGATAA GTATGGTGGA 
ACCTCAATTC AACTCCAGTT GAAAGAGAAG AAGAAAAAAG GATATTGTGA ATGTTGCTTG 
CAGAAATATG AAGATCTAGA AACTCACCTT CTAAGTGAGC AACACAGAAA CTTTGCACAG 
AGTAACCAGT ATCAAGTTGT TGATGATATT GTATCTAAGT TAGTTTTTGA CTTTGTGGAA 
TATGAAAAGG ACACACCTAA AAAGAAAAGA ATAAAATACA GTGTTGGATC CCTTTCTCCT 
GTTTCTGCAA GTGTCCTGAA AAAGACTGAA CAAAAGGAAA AAGTGGAATT GCAACATATT 
TCTCAGAAAG ATTGCCAGGA AGATGATACA ACAGTGAAGG AGCAGAATTT CCTGTATAAA 
GAGACCCAGG AAACTGAAAA AAAGCTCCTG TTTATTTCAG AGCCCATCCC CCACCCTTCA 
AATGAATTGA GAGGGCTTAA TGAGAAAATG AGTAATAAAT GTTCCATGTT AAGTACAGCT 
GAAGATGACA TAAGACAGAA TTTTACACAG CTACCTCTAC ATAAAAACAA ACAGGAATGC 
ATTCTTGACA TTTCCGAACA CACATTAAGT GAAAATGACT TAGAAGAACT AAGGGTAGAT 
CACTATAAAT GTAACATACA GGCATCTGTA CATGTTTCTG ATTTCAGTAC AGATAATAGT 
GGATCTCAAC CAAAACAGAA GTCAGATACT GTGCTTTTTC CAGCAAAGGA TCTCAAGGAA 
AAGGACCTTC ATTCAATATT TACTCATGAT TCTGGTCTGA TAACAATAAA CAGTTCACAA 
GAGCACCTAA CTGTTCAGGC AAAGGCTCCA TTCCATACTC CTCCTGAGGA ACCCAATGAA 
TGTGACTTCA AGAATATGGA TAGTTTACCT TCTGGTAAAA TACATCGAAA AGTGAAAATA 
ATATTAGGAC GAAATAGAAA AGAAAATCTG GAACCAAATG CTGAATTTGA TAAAAGAACT 
GAATTTATTA CACAAGAAGA AAACAGAATT TGTAGTTCAC CGGTACAGTC TTTACTAGAC 
TTGTTTCAGA CTAGTGAAGA GAAATCAGAA TTTTTGGGTT TCACAAGCTA CACAGAAAAG 
AGTGGTATAT GCAATGTTTT AGATATTTGG GAAGAGGAAA ATTCAGATAA TCTGTTAACA 
GCGTTTTTCT CGTCCCCTTC AACTTCTACA TTTACTGGCT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006707.1
CDS	505..2529
Translation	MNSGAMRIHSKGHFQGGIQVKNEKNRPSLKSLKTDNRPEKSKCKPLWGKVFYLDLPSVTISEKLQKDIKDLGGRVEEFLSKDISYLISNKKEAKFAQTLGRISPVPSPESAYTAETTSPHPSHDGSSFKSPDTVCLSRGKLLVEKAIKDHDFIPSNSILSNALSWGVKILHIDDIRYYIEQKKKELYLLKKSSTSVRDGGKRVGSGAQKTRTGRLKKPFVKVEDMSQLYRPFYLQLTNMPFINYSIQKPCSPFDVDKPSSMQKQTQVKLRIQTDGDKYGGTSIQLQLKEKKKKGYCECCLQKYEDLETHLLSEQHRNFAQSNQYQVVDDIVSKLVFDFVEYEKDTPKKKRIKYSVGSLSPVSASVLKKTEQKEKVELQHISQKDCQEDDTTVKEQNFLYKETQETEKKLLFISEPIPHPSNELRGLNEKMSNKCSMLSTAEDDIRQNFTQLPLHKNKQECILDISEHTLSENDLEELRVDHYKCNIQASVHVSDFSTDNSGSQPKQKSDTVLFPAKDLKEKDLHSIFTHDSGLITINSSQEHLTVQAKAPFHTPPEEPNECDFKNMDSLPSGKIHRKVKIILGRNRKENLEPNAEFDKRTEFITQEENRICSSPVQSLLDLFQTSEEKSEFLGFTSYTEKSGICNVLDIWEEENSDNLLTAFFSSPSTSTFTGF

Target ORF information:

RefSeq Version	NM_006716.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DBF4 zinc finger (DBF4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006716.3

ORF Insert Sequence:

ATGAACTCCG GAGCCATGAG GATCCACAGT AAAGGACATT TCCAGGGTGG AATCCAAGTC 
AAAAATGAAA AAAACAGACC ATCTCTGAAA TCTCTGAAAA CTGATAACAG GCCAGAAAAA 
TCCAAATGTA AGCCACTTTG GGGAAAAGTA TTTTACCTTG ACTTACCTTC TGTCACCATA 
TCTGAAAAAC TTCAAAAGGA CATTAAGGAT CTGGGAGGGC GAGTTGAAGA ATTTCTCAGC 
AAAGATATCA GTTATCTTAT TTCAAATAAG AAGGAAGCTA AATTTGCACA AACCTTGGGT 
CGAATTTCTC CTGTACCAAG TCCAGAATCT GCATATACTG CAGAAACCAC TTCACCTCAT 
CCCAGCCATG ATGGAAGTTC ATTTAAGTCA CCAGACACAG TGTGTTTAAG CAGAGGAAAA 
TTATTAGTTG AAAAAGCTAT CAAGGACCAT GATTTTATTC CTTCAAATAG TATATTATCA 
AATGCCTTGT CATGGGGAGT AAAAATTCTT CATATTGATG ACATTAGATA CTACATTGAA 
CAAAAGAAAA AAGAGTTGTA TTTACTCAAG AAATCAAGTA CTTCAGTAAG AGATGGGGGC 
AAAAGAGTTG GTAGTGGTGC ACAAAAAACA AGAACAGGAA GACTCAAAAA GCCTTTTGTA 
AAGGTGGAAG ATATGAGCCA ACTTTATAGG CCATTTTATC TTCAGCTGAC CAATATGCCT 
TTTATAAATT ATTCTATTCA GAAGCCCTGC AGTCCATTTG ATGTAGACAA GCCATCTAGT 
ATGCAAAAGC AAACTCAGGT TAAACTAAGA ATCCAAACAG ATGGCGATAA GTATGGTGGA 
ACCTCAATTC AACTCCAGTT GAAAGAGAAG AAGAAAAAAG GATATTGTGA ATGTTGCTTG 
CAGAAATATG AAGATCTAGA AACTCACCTT CTAAGTGAGC AACACAGAAA CTTTGCACAG 
AGTAACCAGT ATCAAGTTGT TGATGATATT GTATCTAAGT TAGTTTTTGA CTTTGTGGAA 
TATGAAAAGG ACACACCTAA AAAGAAAAGA ATAAAATACA GTGTTGGATC CCTTTCTCCT 
GTTTCTGCAA GTGTCCTGAA AAAGACTGAA CAAAAGGAAA AAGTGGAATT GCAACATATT 
TCTCAGAAAG ATTGCCAGGA AGATGATACA ACAGTGAAGG AGCAGAATTT CCTGTATAAA 
GAGACCCAGG AAACTGAAAA AAAGCTCCTG TTTATTTCAG AGCCCATCCC CCACCCTTCA 
AATGAATTGA GAGGGCTTAA TGAGAAAATG AGTAATAAAT GTTCCATGTT AAGTACAGCT 
GAAGATGACA TAAGACAGAA TTTTACACAG CTACCTCTAC ATAAAAACAA ACAGGAATGC 
ATTCTTGACA TTTCCGAACA CACATTAAGT GAAAATGACT TAGAAGAACT AAGGGTAGAT 
CACTATAAAT GTAACATACA GGCATCTGTA CATGTTTCTG ATTTCAGTAC AGATAATAGT 
GGATCTCAAC CAAAACAGAA GTCAGATACT GTGCTTTTTC CAGCAAAGGA TCTCAAGGAA 
AAGGACCTTC ATTCAATATT TACTCATGAT TCTGGTCTGA TAACAATAAA CAGTTCACAA 
GAGCACCTAA CTGTTCAGGC AAAGGCTCCA TTCCATACTC CTCCTGAGGA ACCCAATGAA 
TGTGACTTCA AGAATATGGA TAGTTTACCT TCTGGTAAAA TACATCGAAA AGTGAAAATA 
ATATTAGGAC GAAATAGAAA AGAAAATCTG GAACCAAATG CTGAATTTGA TAAAAGAACT 
GAATTTATTA CACAAGAAGA AAACAGAATT TGTAGTTCAC CGGTACAGTC TTTACTAGAC 
TTGTTTCAGA CTAGTGAAGA GAAATCAGAA TTTTTGGGTT TCACAAGCTA CACAGAAAAG 
AGTGGTATAT GCAATGTTTT AGATATTTGG GAAGAGGAAA ATTCAGATAA TCTGTTAACA 
GCGTTTTTCT CGTCCCCTTC AACTTCTACA TTTACTGGCT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DBF4 ( NM_001318061.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001304990.1 Homo sapiens DBF4 zinc finger (DBF4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu41348
Clone ID Related Accession (Same CDS sequence)	NM_001318061.1 , NM_001318061.2
Accession Version	NM_001318061.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-02
Organism	Homo sapiens(human)
Product	protein DBF4 homolog A isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB028069.1, BC035632.1 and BC036045.1. On Dec 22, 2015 this sequence version replaced XM_005250121.2. Transcript Variant: This variant (3) has multiple differences, one of which results in a translational frameshift, compared to variant 1. The encoded isoform (3) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC035632.1, AK303175.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCAAC TTTATAGGCC ATTTTATCTT CAGCTGACCA ATATGCCTTT TATAAATTAT 
TCTATTCAGA AGCCCTGCAG TCCATTTGAT GTAGACAAGC CATCTAGTAT GCAAAAGCAA 
ACTCAGGTTA AACTAAGAAT CCAAACAGAT GGCGATAAGT ATGGTGGAAC CTCAATTCAA 
CTCCAGTTGA AAGAGAAGAA GAAAAAAGGA TATTGTGAAT GTTGCTTGCA GAAATATGAA 
GATCTAGAAA CTCACCTTCT AAGTGAGCAA CACAGAAACT TTGCACAGAG TAACCAGTAT 
CAAGTTGTTG ATGATATTGT ATCTAAGTTA GTTTTTGACT TTGTGGAATA TGAAAAGGAC 
ACACCTAAAA AGAAAAGAAT AAAATACAGT GTTGGATCCC TTTCTCCTGT TTCTGCAAGT 
GTCCTGAAAA AGACTGAACA AAAGGAAAAA GTGGAATTGC AACATATTTC TCAGAAAGAT 
TGCCAGGAAG ATGATACAAC AGTGAAGGAG CAGAATTTCC TGTATAAAGA GACCCAGGAA 
ACTGAAAAAA AGCTCCTGTT TATTTCAGAG CCCATCCCCC ACCCTTCAAA TGAATTGAGA 
GGGCTTAATG AGAAAATGAG TAATAAATGT TCCATGTTAA GTACAGCTGA AGATGACATA 
AGACAGAATT TTACACAGCT ACCTCTACAT AAAAACAAAC AGGAATGCAT TCTTGACATT 
TCCGAACACA CATTAAGTGA AAATGACTTA GAAGAACTAA GGGTAGATCA CTATAAATGT 
AACATACAGG CATCTGTACA TGTTTCTGAT TTCAGTACAG ATAATAGTGG ATCTCAACCA 
AAACAGAAGT CAGATACTGT GCTTTTTCCA GCAAAGGATC TCAAGGAAAA GGACCTTCAT 
TCAATATTTA CTCATGATTC TGGTCTGATA ACAATAAACA GTTCACAAGA GCACCTAACT 
GTTCAGGCAA AGGCTCCATT CCATACTCCT CCTGAGGAAC CCAATGAATG TGACTTCAAG 
AATATGGATA GTTTACCTTC TGGTAAAATA CATCGAAAAG TGAAAATAAT ATTAGGACGA 
AATAGAAAAG AAAATCTGGA ACCAAATGCT GAATTTGATA AAAGAACTGA ATTTATTACA 
CAAGAAGAAA ACAGAATTTG TAGTTCACCG GTACAGTCTT TACTAGACTT GTTTCAGACT 
AGTGAAGAGA AATCAGAATT TTTGGGTTTC ACAAGCTACA CAGAAAAGAG TGGTATATGC 
AATGTTTTAG ATATTTGGGA AGAGGAAAAT TCAGATAATC TGTTAACAGC GTTTTTCTCG 
TCCCCTTCAA CTTCTACATT TACTGGCTTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304990.1
CDS	1085..2437
Translation	MSQLYRPFYLQLTNMPFINYSIQKPCSPFDVDKPSSMQKQTQVKLRIQTDGDKYGGTSIQLQLKEKKKKGYCECCLQKYEDLETHLLSEQHRNFAQSNQYQVVDDIVSKLVFDFVEYEKDTPKKKRIKYSVGSLSPVSASVLKKTEQKEKVELQHISQKDCQEDDTTVKEQNFLYKETQETEKKLLFISEPIPHPSNELRGLNEKMSNKCSMLSTAEDDIRQNFTQLPLHKNKQECILDISEHTLSENDLEELRVDHYKCNIQASVHVSDFSTDNSGSQPKQKSDTVLFPAKDLKEKDLHSIFTHDSGLITINSSQEHLTVQAKAPFHTPPEEPNECDFKNMDSLPSGKIHRKVKIILGRNRKENLEPNAEFDKRTEFITQEENRICSSPVQSLLDLFQTSEEKSEFLGFTSYTEKSGICNVLDIWEEENSDNLLTAFFSSPSTSTFTGF

Target ORF information:

RefSeq Version	NM_001318061.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DBF4 zinc finger (DBF4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318061.1

ORF Insert Sequence:

ATGAGCCAAC TTTATAGGCC ATTTTATCTT CAGCTGACCA ATATGCCTTT TATAAATTAT 
TCTATTCAGA AGCCCTGCAG TCCATTTGAT GTAGACAAGC CATCTAGTAT GCAAAAGCAA 
ACTCAGGTTA AACTAAGAAT CCAAACAGAT GGCGATAAGT ATGGTGGAAC CTCAATTCAA 
CTCCAGTTGA AAGAGAAGAA GAAAAAAGGA TATTGTGAAT GTTGCTTGCA GAAATATGAA 
GATCTAGAAA CTCACCTTCT AAGTGAGCAA CACAGAAACT TTGCACAGAG TAACCAGTAT 
CAAGTTGTTG ATGATATTGT ATCTAAGTTA GTTTTTGACT TTGTGGAATA TGAAAAGGAC 
ACACCTAAAA AGAAAAGAAT AAAATACAGT GTTGGATCCC TTTCTCCTGT TTCTGCAAGT 
GTCCTGAAAA AGACTGAACA AAAGGAAAAA GTGGAATTGC AACATATTTC TCAGAAAGAT 
TGCCAGGAAG ATGATACAAC AGTGAAGGAG CAGAATTTCC TGTATAAAGA GACCCAGGAA 
ACTGAAAAAA AGCTCCTGTT TATTTCAGAG CCCATCCCCC ACCCTTCAAA TGAATTGAGA 
GGGCTTAATG AGAAAATGAG TAATAAATGT TCCATGTTAA GTACAGCTGA AGATGACATA 
AGACAGAATT TTACACAGCT ACCTCTACAT AAAAACAAAC AGGAATGCAT TCTTGACATT 
TCCGAACACA CATTAAGTGA AAATGACTTA GAAGAACTAA GGGTAGATCA CTATAAATGT 
AACATACAGG CATCTGTACA TGTTTCTGAT TTCAGTACAG ATAATAGTGG ATCTCAACCA 
AAACAGAAGT CAGATACTGT GCTTTTTCCA GCAAAGGATC TCAAGGAAAA GGACCTTCAT 
TCAATATTTA CTCATGATTC TGGTCTGATA ACAATAAACA GTTCACAAGA GCACCTAACT 
GTTCAGGCAA AGGCTCCATT CCATACTCCT CCTGAGGAAC CCAATGAATG TGACTTCAAG 
AATATGGATA GTTTACCTTC TGGTAAAATA CATCGAAAAG TGAAAATAAT ATTAGGACGA 
AATAGAAAAG AAAATCTGGA ACCAAATGCT GAATTTGATA AAAGAACTGA ATTTATTACA 
CAAGAAGAAA ACAGAATTTG TAGTTCACCG GTACAGTCTT TACTAGACTT GTTTCAGACT 
AGTGAAGAGA AATCAGAATT TTTGGGTTTC ACAAGCTACA CAGAAAAGAG TGGTATATGC 
AATGTTTTAG ATATTTGGGA AGAGGAAAAT TCAGATAATC TGTTAACAGC GTTTTTCTCG 
TCCCCTTCAA CTTCTACATT TACTGGCTTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DBF4 ( NM_001318061.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001304990.1 Homo sapiens DBF4 zinc finger (DBF4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu41348
Clone ID Related Accession (Same CDS sequence)	NM_001318061.1 , NM_001318061.2
Accession Version	NM_001318061.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	protein DBF4 homolog A isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB028069.1, BC035632.1, BC036045.1 and AC005164.2. On May 31, 2019 this sequence version replaced NM_001318061.1. Transcript Variant: This variant (3) has multiple differences, one of which results in a translational frameshift, compared to variant 1. The encoded isoform (3) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC035632.1, AK303175.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCAAC TTTATAGGCC ATTTTATCTT CAGCTGACCA ATATGCCTTT TATAAATTAT 
TCTATTCAGA AGCCCTGCAG TCCATTTGAT GTAGACAAGC CATCTAGTAT GCAAAAGCAA 
ACTCAGGTTA AACTAAGAAT CCAAACAGAT GGCGATAAGT ATGGTGGAAC CTCAATTCAA 
CTCCAGTTGA AAGAGAAGAA GAAAAAAGGA TATTGTGAAT GTTGCTTGCA GAAATATGAA 
GATCTAGAAA CTCACCTTCT AAGTGAGCAA CACAGAAACT TTGCACAGAG TAACCAGTAT 
CAAGTTGTTG ATGATATTGT ATCTAAGTTA GTTTTTGACT TTGTGGAATA TGAAAAGGAC 
ACACCTAAAA AGAAAAGAAT AAAATACAGT GTTGGATCCC TTTCTCCTGT TTCTGCAAGT 
GTCCTGAAAA AGACTGAACA AAAGGAAAAA GTGGAATTGC AACATATTTC TCAGAAAGAT 
TGCCAGGAAG ATGATACAAC AGTGAAGGAG CAGAATTTCC TGTATAAAGA GACCCAGGAA 
ACTGAAAAAA AGCTCCTGTT TATTTCAGAG CCCATCCCCC ACCCTTCAAA TGAATTGAGA 
GGGCTTAATG AGAAAATGAG TAATAAATGT TCCATGTTAA GTACAGCTGA AGATGACATA 
AGACAGAATT TTACACAGCT ACCTCTACAT AAAAACAAAC AGGAATGCAT TCTTGACATT 
TCCGAACACA CATTAAGTGA AAATGACTTA GAAGAACTAA GGGTAGATCA CTATAAATGT 
AACATACAGG CATCTGTACA TGTTTCTGAT TTCAGTACAG ATAATAGTGG ATCTCAACCA 
AAACAGAAGT CAGATACTGT GCTTTTTCCA GCAAAGGATC TCAAGGAAAA GGACCTTCAT 
TCAATATTTA CTCATGATTC TGGTCTGATA ACAATAAACA GTTCACAAGA GCACCTAACT 
GTTCAGGCAA AGGCTCCATT CCATACTCCT CCTGAGGAAC CCAATGAATG TGACTTCAAG 
AATATGGATA GTTTACCTTC TGGTAAAATA CATCGAAAAG TGAAAATAAT ATTAGGACGA 
AATAGAAAAG AAAATCTGGA ACCAAATGCT GAATTTGATA AAAGAACTGA ATTTATTACA 
CAAGAAGAAA ACAGAATTTG TAGTTCACCG GTACAGTCTT TACTAGACTT GTTTCAGACT 
AGTGAAGAGA AATCAGAATT TTTGGGTTTC ACAAGCTACA CAGAAAAGAG TGGTATATGC 
AATGTTTTAG ATATTTGGGA AGAGGAAAAT TCAGATAATC TGTTAACAGC GTTTTTCTCG 
TCCCCTTCAA CTTCTACATT TACTGGCTTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304990.1
CDS	821..2173
Translation	MSQLYRPFYLQLTNMPFINYSIQKPCSPFDVDKPSSMQKQTQVKLRIQTDGDKYGGTSIQLQLKEKKKKGYCECCLQKYEDLETHLLSEQHRNFAQSNQYQVVDDIVSKLVFDFVEYEKDTPKKKRIKYSVGSLSPVSASVLKKTEQKEKVELQHISQKDCQEDDTTVKEQNFLYKETQETEKKLLFISEPIPHPSNELRGLNEKMSNKCSMLSTAEDDIRQNFTQLPLHKNKQECILDISEHTLSENDLEELRVDHYKCNIQASVHVSDFSTDNSGSQPKQKSDTVLFPAKDLKEKDLHSIFTHDSGLITINSSQEHLTVQAKAPFHTPPEEPNECDFKNMDSLPSGKIHRKVKIILGRNRKENLEPNAEFDKRTEFITQEENRICSSPVQSLLDLFQTSEEKSEFLGFTSYTEKSGICNVLDIWEEENSDNLLTAFFSSPSTSTFTGF

Target ORF information:

RefSeq Version	NM_001318061.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DBF4 zinc finger (DBF4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318061.2

ORF Insert Sequence:

ATGAGCCAAC TTTATAGGCC ATTTTATCTT CAGCTGACCA ATATGCCTTT TATAAATTAT 
TCTATTCAGA AGCCCTGCAG TCCATTTGAT GTAGACAAGC CATCTAGTAT GCAAAAGCAA 
ACTCAGGTTA AACTAAGAAT CCAAACAGAT GGCGATAAGT ATGGTGGAAC CTCAATTCAA 
CTCCAGTTGA AAGAGAAGAA GAAAAAAGGA TATTGTGAAT GTTGCTTGCA GAAATATGAA 
GATCTAGAAA CTCACCTTCT AAGTGAGCAA CACAGAAACT TTGCACAGAG TAACCAGTAT 
CAAGTTGTTG ATGATATTGT ATCTAAGTTA GTTTTTGACT TTGTGGAATA TGAAAAGGAC 
ACACCTAAAA AGAAAAGAAT AAAATACAGT GTTGGATCCC TTTCTCCTGT TTCTGCAAGT 
GTCCTGAAAA AGACTGAACA AAAGGAAAAA GTGGAATTGC AACATATTTC TCAGAAAGAT 
TGCCAGGAAG ATGATACAAC AGTGAAGGAG CAGAATTTCC TGTATAAAGA GACCCAGGAA 
ACTGAAAAAA AGCTCCTGTT TATTTCAGAG CCCATCCCCC ACCCTTCAAA TGAATTGAGA 
GGGCTTAATG AGAAAATGAG TAATAAATGT TCCATGTTAA GTACAGCTGA AGATGACATA 
AGACAGAATT TTACACAGCT ACCTCTACAT AAAAACAAAC AGGAATGCAT TCTTGACATT 
TCCGAACACA CATTAAGTGA AAATGACTTA GAAGAACTAA GGGTAGATCA CTATAAATGT 
AACATACAGG CATCTGTACA TGTTTCTGAT TTCAGTACAG ATAATAGTGG ATCTCAACCA 
AAACAGAAGT CAGATACTGT GCTTTTTCCA GCAAAGGATC TCAAGGAAAA GGACCTTCAT 
TCAATATTTA CTCATGATTC TGGTCTGATA ACAATAAACA GTTCACAAGA GCACCTAACT 
GTTCAGGCAA AGGCTCCATT CCATACTCCT CCTGAGGAAC CCAATGAATG TGACTTCAAG 
AATATGGATA GTTTACCTTC TGGTAAAATA CATCGAAAAG TGAAAATAAT ATTAGGACGA 
AATAGAAAAG AAAATCTGGA ACCAAATGCT GAATTTGATA AAAGAACTGA ATTTATTACA 
CAAGAAGAAA ACAGAATTTG TAGTTCACCG GTACAGTCTT TACTAGACTT GTTTCAGACT 
AGTGAAGAGA AATCAGAATT TTTGGGTTTC ACAAGCTACA CAGAAAAGAG TGGTATATGC 
AATGTTTTAG ATATTTGGGA AGAGGAAAAT TCAGATAATC TGTTAACAGC GTTTTTCTCG 
TCCCCTTCAA CTTCTACATT TACTGGCTTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DBF4 ( NM_001318062.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001304991.1 Homo sapiens DBF4 zinc finger (DBF4), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu64528
Clone ID Related Accession (Same CDS sequence)	NM_001318062.1 , NM_001318062.2
Accession Version	NM_001318062.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-02
Organism	Homo sapiens(human)
Product	protein DBF4 homolog A isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB028070.1 and BC036045.1. On or before Dec 22, 2015 this sequence version replaced XR_927343.1, XM_011515765.1. Transcript Variant: This variant (4) has multiple differences, one of which results in a translational frameshift, compared to variant 1. The encoded isoform (4) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB028070.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAAAAGC AAACTCAGGT TAAACTAAGA ATCCAAACAG ATGGCGATAA GTATGGTGGA 
ACCTCAATTC AACTCCAGTT GAAAGAGAAG AAGAAAAAAG GATATTGTGA ATGTTGCTTG 
CAGAAATATG AAGATCTAGA AACTCACCTT CTAAGTGAGC AACACAGAAA CTTTGCACAG 
AGTAACCAGT ATCAAGTTGT TGATGATATT GTATCTAAGT TAGTTTTTGA CTTTGTGGAA 
TATGAAAAGG ACACACCTAA AAAGAAAAGA ATAAAATACA GTGTTGGATC CCTTTCTCCT 
GTTTCTGCAA GTGTCCTGAA AAAGACTGAA CAAAAGGAAA AAGTGGAATT GCAACATATT 
TCTCAGAAAG ATTGCCAGGA AGATGATACA ACAGTGAAGG AGCAGAATTT CCTGTATAAA 
GAGACCCAGG AAACTGAAAA AAAGCTCCTG TTTATTTCAG AGCCCATCCC CCACCCTTCA 
AATGAATTGA GAGGGCTTAA TGAGAAAATG AGTAATAAAT GTTCCATGTT AAGTACAGCT 
GAAGATGACA TAAGACAGAA TTTTACACAG CTACCTCTAC ATAAAAACAA ACAGGAATGC 
ATTCTTGACA TTTCCGAACA CACATTAAGT GAAAATGACT TAGAAGAACT AAGGGTAGAT 
CACTATAAAT GTAACATACA GGCATCTGTA CATGTTTCTG ATTTCAGTAC AGATAATAGT 
GGATCTCAAC CAAAACAGAA GTCAGATACT GTGCTTTTTC CAGCAAAGGA TCTCAAGGAA 
AAGGACCTTC ATTCAATATT TACTCATGAT TCTGGTCTGA TAACAATAAA CAGTTCACAA 
GAGCACCTAA CTGTTCAGGC AAAGGCTCCA TTCCATACTC CTCCTGAGGA ACCCAATGAA 
TGTGACTTCA AGAATATGGA TAGTTTACCT TCTGGTAAAA TACATCGAAA AGTGAAAATA 
ATATTAGGAC GAAATAGAAA AGAAAATCTG GAACCAAATG CTGAATTTGA TAAAAGAACT 
GAATTTATTA CACAAGAAGA AAACAGAATT TGTAGTTCAC CGGTACAGTC TTTACTAGAC 
TTGTTTCAGA CTAGTGAAGA GAAATCAGAA TTTTTGGGTT TCACAAGCTA CACAGAAAAG 
AGTGGTATAT GCAATGTTTT AGATATTTGG GAAGAGGAAA ATTCAGATAA TCTGTTAACA 
GCGTTTTTCT CGTCCCCTTC AACTTCTACA TTTACTGGCT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304991.1
CDS	1224..2468
Translation	MQKQTQVKLRIQTDGDKYGGTSIQLQLKEKKKKGYCECCLQKYEDLETHLLSEQHRNFAQSNQYQVVDDIVSKLVFDFVEYEKDTPKKKRIKYSVGSLSPVSASVLKKTEQKEKVELQHISQKDCQEDDTTVKEQNFLYKETQETEKKLLFISEPIPHPSNELRGLNEKMSNKCSMLSTAEDDIRQNFTQLPLHKNKQECILDISEHTLSENDLEELRVDHYKCNIQASVHVSDFSTDNSGSQPKQKSDTVLFPAKDLKEKDLHSIFTHDSGLITINSSQEHLTVQAKAPFHTPPEEPNECDFKNMDSLPSGKIHRKVKIILGRNRKENLEPNAEFDKRTEFITQEENRICSSPVQSLLDLFQTSEEKSEFLGFTSYTEKSGICNVLDIWEEENSDNLLTAFFSSPSTSTFTGF

Target ORF information:

RefSeq Version	NM_001318062.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DBF4 zinc finger (DBF4), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318062.1

ORF Insert Sequence:

ATGCAAAAGC AAACTCAGGT TAAACTAAGA ATCCAAACAG ATGGCGATAA GTATGGTGGA 
ACCTCAATTC AACTCCAGTT GAAAGAGAAG AAGAAAAAAG GATATTGTGA ATGTTGCTTG 
CAGAAATATG AAGATCTAGA AACTCACCTT CTAAGTGAGC AACACAGAAA CTTTGCACAG 
AGTAACCAGT ATCAAGTTGT TGATGATATT GTATCTAAGT TAGTTTTTGA CTTTGTGGAA 
TATGAAAAGG ACACACCTAA AAAGAAAAGA ATAAAATACA GTGTTGGATC CCTTTCTCCT 
GTTTCTGCAA GTGTCCTGAA AAAGACTGAA CAAAAGGAAA AAGTGGAATT GCAACATATT 
TCTCAGAAAG ATTGCCAGGA AGATGATACA ACAGTGAAGG AGCAGAATTT CCTGTATAAA 
GAGACCCAGG AAACTGAAAA AAAGCTCCTG TTTATTTCAG AGCCCATCCC CCACCCTTCA 
AATGAATTGA GAGGGCTTAA TGAGAAAATG AGTAATAAAT GTTCCATGTT AAGTACAGCT 
GAAGATGACA TAAGACAGAA TTTTACACAG CTACCTCTAC ATAAAAACAA ACAGGAATGC 
ATTCTTGACA TTTCCGAACA CACATTAAGT GAAAATGACT TAGAAGAACT AAGGGTAGAT 
CACTATAAAT GTAACATACA GGCATCTGTA CATGTTTCTG ATTTCAGTAC AGATAATAGT 
GGATCTCAAC CAAAACAGAA GTCAGATACT GTGCTTTTTC CAGCAAAGGA TCTCAAGGAA 
AAGGACCTTC ATTCAATATT TACTCATGAT TCTGGTCTGA TAACAATAAA CAGTTCACAA 
GAGCACCTAA CTGTTCAGGC AAAGGCTCCA TTCCATACTC CTCCTGAGGA ACCCAATGAA 
TGTGACTTCA AGAATATGGA TAGTTTACCT TCTGGTAAAA TACATCGAAA AGTGAAAATA 
ATATTAGGAC GAAATAGAAA AGAAAATCTG GAACCAAATG CTGAATTTGA TAAAAGAACT 
GAATTTATTA CACAAGAAGA AAACAGAATT TGTAGTTCAC CGGTACAGTC TTTACTAGAC 
TTGTTTCAGA CTAGTGAAGA GAAATCAGAA TTTTTGGGTT TCACAAGCTA CACAGAAAAG 
AGTGGTATAT GCAATGTTTT AGATATTTGG GAAGAGGAAA ATTCAGATAA TCTGTTAACA 
GCGTTTTTCT CGTCCCCTTC AACTTCTACA TTTACTGGCT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DBF4 ( NM_001318062.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001304991.1 Homo sapiens DBF4 zinc finger (DBF4), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu64528
Clone ID Related Accession (Same CDS sequence)	NM_001318062.1 , NM_001318062.2
Accession Version	NM_001318062.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	protein DBF4 homolog A isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB028070.1, BC036045.1 and AC005164.2. On May 31, 2019 this sequence version replaced NM_001318062.1. Transcript Variant: This variant (4) has multiple differences, one of which results in a translational frameshift, compared to variant 1. The encoded isoform (4) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB028070.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAAAAGC AAACTCAGGT TAAACTAAGA ATCCAAACAG ATGGCGATAA GTATGGTGGA 
ACCTCAATTC AACTCCAGTT GAAAGAGAAG AAGAAAAAAG GATATTGTGA ATGTTGCTTG 
CAGAAATATG AAGATCTAGA AACTCACCTT CTAAGTGAGC AACACAGAAA CTTTGCACAG 
AGTAACCAGT ATCAAGTTGT TGATGATATT GTATCTAAGT TAGTTTTTGA CTTTGTGGAA 
TATGAAAAGG ACACACCTAA AAAGAAAAGA ATAAAATACA GTGTTGGATC CCTTTCTCCT 
GTTTCTGCAA GTGTCCTGAA AAAGACTGAA CAAAAGGAAA AAGTGGAATT GCAACATATT 
TCTCAGAAAG ATTGCCAGGA AGATGATACA ACAGTGAAGG AGCAGAATTT CCTGTATAAA 
GAGACCCAGG AAACTGAAAA AAAGCTCCTG TTTATTTCAG AGCCCATCCC CCACCCTTCA 
AATGAATTGA GAGGGCTTAA TGAGAAAATG AGTAATAAAT GTTCCATGTT AAGTACAGCT 
GAAGATGACA TAAGACAGAA TTTTACACAG CTACCTCTAC ATAAAAACAA ACAGGAATGC 
ATTCTTGACA TTTCCGAACA CACATTAAGT GAAAATGACT TAGAAGAACT AAGGGTAGAT 
CACTATAAAT GTAACATACA GGCATCTGTA CATGTTTCTG ATTTCAGTAC AGATAATAGT 
GGATCTCAAC CAAAACAGAA GTCAGATACT GTGCTTTTTC CAGCAAAGGA TCTCAAGGAA 
AAGGACCTTC ATTCAATATT TACTCATGAT TCTGGTCTGA TAACAATAAA CAGTTCACAA 
GAGCACCTAA CTGTTCAGGC AAAGGCTCCA TTCCATACTC CTCCTGAGGA ACCCAATGAA 
TGTGACTTCA AGAATATGGA TAGTTTACCT TCTGGTAAAA TACATCGAAA AGTGAAAATA 
ATATTAGGAC GAAATAGAAA AGAAAATCTG GAACCAAATG CTGAATTTGA TAAAAGAACT 
GAATTTATTA CACAAGAAGA AAACAGAATT TGTAGTTCAC CGGTACAGTC TTTACTAGAC 
TTGTTTCAGA CTAGTGAAGA GAAATCAGAA TTTTTGGGTT TCACAAGCTA CACAGAAAAG 
AGTGGTATAT GCAATGTTTT AGATATTTGG GAAGAGGAAA ATTCAGATAA TCTGTTAACA 
GCGTTTTTCT CGTCCCCTTC AACTTCTACA TTTACTGGCT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304991.1
CDS	960..2204
Translation	MQKQTQVKLRIQTDGDKYGGTSIQLQLKEKKKKGYCECCLQKYEDLETHLLSEQHRNFAQSNQYQVVDDIVSKLVFDFVEYEKDTPKKKRIKYSVGSLSPVSASVLKKTEQKEKVELQHISQKDCQEDDTTVKEQNFLYKETQETEKKLLFISEPIPHPSNELRGLNEKMSNKCSMLSTAEDDIRQNFTQLPLHKNKQECILDISEHTLSENDLEELRVDHYKCNIQASVHVSDFSTDNSGSQPKQKSDTVLFPAKDLKEKDLHSIFTHDSGLITINSSQEHLTVQAKAPFHTPPEEPNECDFKNMDSLPSGKIHRKVKIILGRNRKENLEPNAEFDKRTEFITQEENRICSSPVQSLLDLFQTSEEKSEFLGFTSYTEKSGICNVLDIWEEENSDNLLTAFFSSPSTSTFTGF

Target ORF information:

RefSeq Version	NM_001318062.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DBF4 zinc finger (DBF4), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318062.2

ORF Insert Sequence:

ATGCAAAAGC AAACTCAGGT TAAACTAAGA ATCCAAACAG ATGGCGATAA GTATGGTGGA 
ACCTCAATTC AACTCCAGTT GAAAGAGAAG AAGAAAAAAG GATATTGTGA ATGTTGCTTG 
CAGAAATATG AAGATCTAGA AACTCACCTT CTAAGTGAGC AACACAGAAA CTTTGCACAG 
AGTAACCAGT ATCAAGTTGT TGATGATATT GTATCTAAGT TAGTTTTTGA CTTTGTGGAA 
TATGAAAAGG ACACACCTAA AAAGAAAAGA ATAAAATACA GTGTTGGATC CCTTTCTCCT 
GTTTCTGCAA GTGTCCTGAA AAAGACTGAA CAAAAGGAAA AAGTGGAATT GCAACATATT 
TCTCAGAAAG ATTGCCAGGA AGATGATACA ACAGTGAAGG AGCAGAATTT CCTGTATAAA 
GAGACCCAGG AAACTGAAAA AAAGCTCCTG TTTATTTCAG AGCCCATCCC CCACCCTTCA 
AATGAATTGA GAGGGCTTAA TGAGAAAATG AGTAATAAAT GTTCCATGTT AAGTACAGCT 
GAAGATGACA TAAGACAGAA TTTTACACAG CTACCTCTAC ATAAAAACAA ACAGGAATGC 
ATTCTTGACA TTTCCGAACA CACATTAAGT GAAAATGACT TAGAAGAACT AAGGGTAGAT 
CACTATAAAT GTAACATACA GGCATCTGTA CATGTTTCTG ATTTCAGTAC AGATAATAGT 
GGATCTCAAC CAAAACAGAA GTCAGATACT GTGCTTTTTC CAGCAAAGGA TCTCAAGGAA 
AAGGACCTTC ATTCAATATT TACTCATGAT TCTGGTCTGA TAACAATAAA CAGTTCACAA 
GAGCACCTAA CTGTTCAGGC AAAGGCTCCA TTCCATACTC CTCCTGAGGA ACCCAATGAA 
TGTGACTTCA AGAATATGGA TAGTTTACCT TCTGGTAAAA TACATCGAAA AGTGAAAATA 
ATATTAGGAC GAAATAGAAA AGAAAATCTG GAACCAAATG CTGAATTTGA TAAAAGAACT 
GAATTTATTA CACAAGAAGA AAACAGAATT TGTAGTTCAC CGGTACAGTC TTTACTAGAC 
TTGTTTCAGA CTAGTGAAGA GAAATCAGAA TTTTTGGGTT TCACAAGCTA CACAGAAAAG 
AGTGGTATAT GCAATGTTTT AGATATTTGG GAAGAGGAAA ATTCAGATAA TCTGTTAACA 
GCGTTTTTCT CGTCCCCTTC AACTTCTACA TTTACTGGCT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DBF4 ( NM_001318060.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001304989.1 Homo sapiens DBF4 zinc finger (DBF4), transcript variant 2, mRNA.

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CloneID	OHu101514
Clone ID Related Accession (Same CDS sequence)	NM_001318060.1 , NM_001318060.2
Accession Version	NM_001318060.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-02
Organism	Homo sapiens(human)
Product	protein DBF4 homolog A isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB028069.1, AK302010.1, BC014909.1 and BC036045.1. Transcript Variant: This variant (2) has multiple differences in the coding region, one of which results in a translational frameshift, compared to variant 1. The encoded isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302010.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGCAA AAGAGTTGGT AGTGGTGCAC AAAAAACAAG AAAAGCCCTG CAGTCCATTT 
GATGTAGACA AGCCATCTAG TATGCAAAAG CAAACTCAGG TTAAACTAAG AATCCAAACA 
GATGGCGATA AGTATGGTGG AACCTCAATT CAACTCCAGT TGAAAGAGAA GAAGAAAAAA 
GGATATTGTG AATGTTGCTT GCAGAAATAT GAAGATCTAG AAACTCACCT TCTAAGTGAG 
CAACACAGAA ACTTTGCACA GAGTAACCAG TATCAAGTTG TTGATGATAT TGTATCTAAG 
TTAGTTTTTG ACTTTGTGGA ATATGAAAAG GACACACCTA AAAAGAAAAG AATAAAATAC 
AGTGTTGGAT CCCTTTCTCC TGTTTCTGCA AGTGTCCTGA AAAAGACTGA ACAAAAGGAA 
AAAGTGGAAT TGCAACATAT TTCTCAGAAA GATTGCCAGG AAGATGATAC AACAGTGAAG 
GAGCAGAATT TCCTGTATAA AGAGACCCAG GAAACTGAAA AAAAGCTCCT GTTTATTTCA 
GAGCCCATCC CCCACCCTTC AAATGAATTG AGAGGGCTTA ATGAGAAAAT GAGTAATAAA 
TGTTCCATGT TAAGTACAGC TGAAGATGAC ATAAGACAGA ATTTTACACA GCTACCTCTA 
CATAAAAACA AACAGGAATG CATTCTTGAC ATTTCCGAAC ACACATTAAG TGAAAATGAC 
TTAGAAGAAC TAAGGGTAGA TCACTATAAA TGTAACATAC AGGCATCTGT ACATGTTTCT 
GATTTCAGTA CAGATAATAG TGGATCTCAA CCAAAACAGA AGTCAGATAC TGTGCTTTTT 
CCAGCAAAGG ATCTCAAGGA AAAGGACCTT CATTCAATAT TTACTCATGA TTCTGGTCTG 
ATAACAATAA ACAGTTCACA AGAGCACCTA ACTGTTCAGG CAAAGGCTCC ATTCCATACT 
CCTCCTGAGG AACCCAATGA ATGTGACTTC AAGAATATGG ATAGTTTACC TTCTGGTAAA 
ATACATCGAA AAGTGAAAAT AATATTAGGA CGAAATAGAA AAGAAAATCT GGAACCAAAT 
GCTGAATTTG ATAAAAGAAC TGAATTTATT ACACAAGAAG AAAACAGAAT TTGTAGTTCA 
CCGGTACAGT CTTTACTAGA CTTGTTTCAG ACTAGTGAAG AGAAATCAGA ATTTTTGGGT 
TTCACAAGCT ACACAGAAAA GAGTGGTATA TGCAATGTTT TAGATATTTG GGAAGAGGAA 
AATTCAGATA ATCTGTTAAC AGCGTTTTTC TCGTCCCCTT CAACTTCTAC ATTTACTGGC 
TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304989.1
CDS	1097..2422
Translation	MGAKELVVVHKKQEKPCSPFDVDKPSSMQKQTQVKLRIQTDGDKYGGTSIQLQLKEKKKKGYCECCLQKYEDLETHLLSEQHRNFAQSNQYQVVDDIVSKLVFDFVEYEKDTPKKKRIKYSVGSLSPVSASVLKKTEQKEKVELQHISQKDCQEDDTTVKEQNFLYKETQETEKKLLFISEPIPHPSNELRGLNEKMSNKCSMLSTAEDDIRQNFTQLPLHKNKQECILDISEHTLSENDLEELRVDHYKCNIQASVHVSDFSTDNSGSQPKQKSDTVLFPAKDLKEKDLHSIFTHDSGLITINSSQEHLTVQAKAPFHTPPEEPNECDFKNMDSLPSGKIHRKVKIILGRNRKENLEPNAEFDKRTEFITQEENRICSSPVQSLLDLFQTSEEKSEFLGFTSYTEKSGICNVLDIWEEENSDNLLTAFFSSPSTSTFTGF

Target ORF information:

RefSeq Version	NM_001318060.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DBF4 zinc finger (DBF4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318060.1

ORF Insert Sequence:

ATGGGGGCAA AAGAGTTGGT AGTGGTGCAC AAAAAACAAG AAAAGCCCTG CAGTCCATTT 
GATGTAGACA AGCCATCTAG TATGCAAAAG CAAACTCAGG TTAAACTAAG AATCCAAACA 
GATGGCGATA AGTATGGTGG AACCTCAATT CAACTCCAGT TGAAAGAGAA GAAGAAAAAA 
GGATATTGTG AATGTTGCTT GCAGAAATAT GAAGATCTAG AAACTCACCT TCTAAGTGAG 
CAACACAGAA ACTTTGCACA GAGTAACCAG TATCAAGTTG TTGATGATAT TGTATCTAAG 
TTAGTTTTTG ACTTTGTGGA ATATGAAAAG GACACACCTA AAAAGAAAAG AATAAAATAC 
AGTGTTGGAT CCCTTTCTCC TGTTTCTGCA AGTGTCCTGA AAAAGACTGA ACAAAAGGAA 
AAAGTGGAAT TGCAACATAT TTCTCAGAAA GATTGCCAGG AAGATGATAC AACAGTGAAG 
GAGCAGAATT TCCTGTATAA AGAGACCCAG GAAACTGAAA AAAAGCTCCT GTTTATTTCA 
GAGCCCATCC CCCACCCTTC AAATGAATTG AGAGGGCTTA ATGAGAAAAT GAGTAATAAA 
TGTTCCATGT TAAGTACAGC TGAAGATGAC ATAAGACAGA ATTTTACACA GCTACCTCTA 
CATAAAAACA AACAGGAATG CATTCTTGAC ATTTCCGAAC ACACATTAAG TGAAAATGAC 
TTAGAAGAAC TAAGGGTAGA TCACTATAAA TGTAACATAC AGGCATCTGT ACATGTTTCT 
GATTTCAGTA CAGATAATAG TGGATCTCAA CCAAAACAGA AGTCAGATAC TGTGCTTTTT 
CCAGCAAAGG ATCTCAAGGA AAAGGACCTT CATTCAATAT TTACTCATGA TTCTGGTCTG 
ATAACAATAA ACAGTTCACA AGAGCACCTA ACTGTTCAGG CAAAGGCTCC ATTCCATACT 
CCTCCTGAGG AACCCAATGA ATGTGACTTC AAGAATATGG ATAGTTTACC TTCTGGTAAA 
ATACATCGAA AAGTGAAAAT AATATTAGGA CGAAATAGAA AAGAAAATCT GGAACCAAAT 
GCTGAATTTG ATAAAAGAAC TGAATTTATT ACACAAGAAG AAAACAGAAT TTGTAGTTCA 
CCGGTACAGT CTTTACTAGA CTTGTTTCAG ACTAGTGAAG AGAAATCAGA ATTTTTGGGT 
TTCACAAGCT ACACAGAAAA GAGTGGTATA TGCAATGTTT TAGATATTTG GGAAGAGGAA 
AATTCAGATA ATCTGTTAAC AGCGTTTTTC TCGTCCCCTT CAACTTCTAC ATTTACTGGC 
TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DBF4 ( NM_001318060.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001304989.1 Homo sapiens DBF4 zinc finger (DBF4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu101514
Clone ID Related Accession (Same CDS sequence)	NM_001318060.1 , NM_001318060.2
Accession Version	NM_001318060.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	protein DBF4 homolog A isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB028069.1, AK302010.1, BC014909.1, BC036045.1 and AC005164.2. On May 31, 2019 this sequence version replaced NM_001318060.1. Transcript Variant: This variant (2) has multiple differences in the coding region, one of which results in a translational frameshift, compared to variant 1. The encoded isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302010.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGCAA AAGAGTTGGT AGTGGTGCAC AAAAAACAAG AAAAGCCCTG CAGTCCATTT 
GATGTAGACA AGCCATCTAG TATGCAAAAG CAAACTCAGG TTAAACTAAG AATCCAAACA 
GATGGCGATA AGTATGGTGG AACCTCAATT CAACTCCAGT TGAAAGAGAA GAAGAAAAAA 
GGATATTGTG AATGTTGCTT GCAGAAATAT GAAGATCTAG AAACTCACCT TCTAAGTGAG 
CAACACAGAA ACTTTGCACA GAGTAACCAG TATCAAGTTG TTGATGATAT TGTATCTAAG 
TTAGTTTTTG ACTTTGTGGA ATATGAAAAG GACACACCTA AAAAGAAAAG AATAAAATAC 
AGTGTTGGAT CCCTTTCTCC TGTTTCTGCA AGTGTCCTGA AAAAGACTGA ACAAAAGGAA 
AAAGTGGAAT TGCAACATAT TTCTCAGAAA GATTGCCAGG AAGATGATAC AACAGTGAAG 
GAGCAGAATT TCCTGTATAA AGAGACCCAG GAAACTGAAA AAAAGCTCCT GTTTATTTCA 
GAGCCCATCC CCCACCCTTC AAATGAATTG AGAGGGCTTA ATGAGAAAAT GAGTAATAAA 
TGTTCCATGT TAAGTACAGC TGAAGATGAC ATAAGACAGA ATTTTACACA GCTACCTCTA 
CATAAAAACA AACAGGAATG CATTCTTGAC ATTTCCGAAC ACACATTAAG TGAAAATGAC 
TTAGAAGAAC TAAGGGTAGA TCACTATAAA TGTAACATAC AGGCATCTGT ACATGTTTCT 
GATTTCAGTA CAGATAATAG TGGATCTCAA CCAAAACAGA AGTCAGATAC TGTGCTTTTT 
CCAGCAAAGG ATCTCAAGGA AAAGGACCTT CATTCAATAT TTACTCATGA TTCTGGTCTG 
ATAACAATAA ACAGTTCACA AGAGCACCTA ACTGTTCAGG CAAAGGCTCC ATTCCATACT 
CCTCCTGAGG AACCCAATGA ATGTGACTTC AAGAATATGG ATAGTTTACC TTCTGGTAAA 
ATACATCGAA AAGTGAAAAT AATATTAGGA CGAAATAGAA AAGAAAATCT GGAACCAAAT 
GCTGAATTTG ATAAAAGAAC TGAATTTATT ACACAAGAAG AAAACAGAAT TTGTAGTTCA 
CCGGTACAGT CTTTACTAGA CTTGTTTCAG ACTAGTGAAG AGAAATCAGA ATTTTTGGGT 
TTCACAAGCT ACACAGAAAA GAGTGGTATA TGCAATGTTT TAGATATTTG GGAAGAGGAA 
AATTCAGATA ATCTGTTAAC AGCGTTTTTC TCGTCCCCTT CAACTTCTAC ATTTACTGGC 
TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304989.1
CDS	833..2158
Translation	MGAKELVVVHKKQEKPCSPFDVDKPSSMQKQTQVKLRIQTDGDKYGGTSIQLQLKEKKKKGYCECCLQKYEDLETHLLSEQHRNFAQSNQYQVVDDIVSKLVFDFVEYEKDTPKKKRIKYSVGSLSPVSASVLKKTEQKEKVELQHISQKDCQEDDTTVKEQNFLYKETQETEKKLLFISEPIPHPSNELRGLNEKMSNKCSMLSTAEDDIRQNFTQLPLHKNKQECILDISEHTLSENDLEELRVDHYKCNIQASVHVSDFSTDNSGSQPKQKSDTVLFPAKDLKEKDLHSIFTHDSGLITINSSQEHLTVQAKAPFHTPPEEPNECDFKNMDSLPSGKIHRKVKIILGRNRKENLEPNAEFDKRTEFITQEENRICSSPVQSLLDLFQTSEEKSEFLGFTSYTEKSGICNVLDIWEEENSDNLLTAFFSSPSTSTFTGF

Target ORF information:

RefSeq Version	NM_001318060.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DBF4 zinc finger (DBF4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318060.2

ORF Insert Sequence:

ATGGGGGCAA AAGAGTTGGT AGTGGTGCAC AAAAAACAAG AAAAGCCCTG CAGTCCATTT 
GATGTAGACA AGCCATCTAG TATGCAAAAG CAAACTCAGG TTAAACTAAG AATCCAAACA 
GATGGCGATA AGTATGGTGG AACCTCAATT CAACTCCAGT TGAAAGAGAA GAAGAAAAAA 
GGATATTGTG AATGTTGCTT GCAGAAATAT GAAGATCTAG AAACTCACCT TCTAAGTGAG 
CAACACAGAA ACTTTGCACA GAGTAACCAG TATCAAGTTG TTGATGATAT TGTATCTAAG 
TTAGTTTTTG ACTTTGTGGA ATATGAAAAG GACACACCTA AAAAGAAAAG AATAAAATAC 
AGTGTTGGAT CCCTTTCTCC TGTTTCTGCA AGTGTCCTGA AAAAGACTGA ACAAAAGGAA 
AAAGTGGAAT TGCAACATAT TTCTCAGAAA GATTGCCAGG AAGATGATAC AACAGTGAAG 
GAGCAGAATT TCCTGTATAA AGAGACCCAG GAAACTGAAA AAAAGCTCCT GTTTATTTCA 
GAGCCCATCC CCCACCCTTC AAATGAATTG AGAGGGCTTA ATGAGAAAAT GAGTAATAAA 
TGTTCCATGT TAAGTACAGC TGAAGATGAC ATAAGACAGA ATTTTACACA GCTACCTCTA 
CATAAAAACA AACAGGAATG CATTCTTGAC ATTTCCGAAC ACACATTAAG TGAAAATGAC 
TTAGAAGAAC TAAGGGTAGA TCACTATAAA TGTAACATAC AGGCATCTGT ACATGTTTCT 
GATTTCAGTA CAGATAATAG TGGATCTCAA CCAAAACAGA AGTCAGATAC TGTGCTTTTT 
CCAGCAAAGG ATCTCAAGGA AAAGGACCTT CATTCAATAT TTACTCATGA TTCTGGTCTG 
ATAACAATAA ACAGTTCACA AGAGCACCTA ACTGTTCAGG CAAAGGCTCC ATTCCATACT 
CCTCCTGAGG AACCCAATGA ATGTGACTTC AAGAATATGG ATAGTTTACC TTCTGGTAAA 
ATACATCGAA AAGTGAAAAT AATATTAGGA CGAAATAGAA AAGAAAATCT GGAACCAAAT 
GCTGAATTTG ATAAAAGAAC TGAATTTATT ACACAAGAAG AAAACAGAAT TTGTAGTTCA 
CCGGTACAGT CTTTACTAGA CTTGTTTCAG ACTAGTGAAG AGAAATCAGA ATTTTTGGGT 
TTCACAAGCT ACACAGAAAA GAGTGGTATA TGCAATGTTT TAGATATTTG GGAAGAGGAA 
AATTCAGATA ATCTGTTAAC AGCGTTTTTC TCGTCCCCTT CAACTTCTAC ATTTACTGGC 
TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cdc7-Dbf4-mediated phosphorylation of HSP90-S164 stabilizes HSP90-HCLK2-MRN complex to enhance ATR/ATM signaling that overcomes replication stress in cancer.
Scientific reports7(1)17024(2017 Dec)
Cheng AN,Fan CC,Lo YK,Kuo CL,Wang HC,Lien IH,Lin SY,Chen CH,Jiang SS,Chang IS,Juan HF,Lyu PC,Lee AY

DDK Promotes Tumor Chemoresistance and Survival via Multiple Pathways.
Neoplasia (New York, N.Y.)19(5)439-450(2017 May)
Sasi NK,Bhutkar A,Lanning NJ,MacKeigan JP,Weinreich M

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

DDK dependent regulation of TOP2A at centromeres revealed by a chemical genetics approach.
Nucleic acids research44(18)8786-8798(2016 Oct)
Wu KZ,Wang GN,Fitzgerald J,Quachthithu H,Rainey MD,Cattaneo A,Bachi A,Santocanale C

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DBF4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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