GTF2A1L cDNA ORF clone, Homo sapiens(human)
Gene Symbol | GTF2A1L |
Entrez Gene ID | 11036 |
Full Name | general transcription factor IIA subunit 1 like |
Synonyms | ALF |
General protein information |
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Gene Type | protein-coding |
Organism | Homo sapiens(human) |
Genome | |
Summary | The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involve the effects of transcription factor IIA (TFIIA) on the interaction between TATA-binding protein (TBP) and DNA. This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology. Alternative splicing for this locus has been observed and two variants, encoding distinct isoforms, have been identified. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Mar 2014]. |
Disorder MIM: |
mRNA and Protein(s)
mRNA | Protein | Name |
---|---|---|
NM_006872.4 | NP_006863.2 | TFIIA-alpha and beta-like factor isoform 1 |
NM_001193487.2 | NP_001180416.1 | TFIIA-alpha and beta-like factor isoform 2 |
NM_006872.5 | NP_006863.2 | TFIIA-alpha and beta-like factor isoform 1 |

Danio rerio (zebrafish) | gtf2a1l | NP_001070039.1 |
Xenopus tropicalis (tropical clawed frog) | gtf2a1l | NP_001039213.1 |
Rattus norvegicus (Norway rat) | Gtf2a1l | NP_001012136.1 |
Canis lupus familiaris (dog) | GTF2A1L | NP_001191772.1 |
Mus musculus (house mouse) | Gtf2a1l | NP_076119.2 |
Gallus gallus (chicken) | GTF2A1L | NP_001191795.1 |
Homo sapiens (human) | GTF2A1L | NP_006863.2 |
Pan troglodytes (chimpanzee) | GTF2A1L | NP_001191755.1 |

Related articles in PubMed
Association of single nucleotide polymorphisms in the USF1, GTF2A1L and OR2W3 genes with non-obstructive azoospermia in the Chinese population.
Zhang Y, He XJ, Song B, Ye L, Xie XS, Ruan J, Zhou FS, Zuo XB, Cao YX, Du WD
Journal of assisted reproduction and genetics32(1)95-101(2015 Jan)
Analysis of copy number variation in Alzheimer's disease in a cohort of clinically characterized and neuropathologically verified individuals.
Swaminathan S, Huentelman MJ, Corneveaux JJ, Myers AJ, Faber KM, Foroud T, Mayeux R, Shen L, Kim S, Turk M, Hardy J, Reiman EM, Saykin AJ,
PloS one7(12)e50640(2012)
Genome-wide association study identifies susceptibility loci for polycystic ovary syndrome on chromosome 2p16.3, 2p21 and 9q33.3.
Chen ZJ, Zhao H, He L, Shi Y, Qin Y, Shi Y, Li Z, You L, Zhao J, Liu J, Liang X, Zhao X, Zhao J, Sun Y, Zhang B, Jiang H, Zhao D, Bian Y, Gao X, Geng L, Li Y, Zhu D, Sun X, Xu JE, Hao C, Ren CE, Zhang Y, Chen S, Zhang W, Yang A, Yan J, Li Y, Ma J, Zhao Y
Nature genetics43(1)55-9(2011 Jan)
Regulatory factor interactions and somatic silencing of the germ cell-specific ALF gene.
Kim M, Li D, Cui Y, Mueller K, Chears WC, DeJong J
The Journal of biological chemistry281(45)34288-98(2006 Nov)
Transcription Factor IIA tau is associated with undifferentiated cells and its gene expression is repressed in primary neurons at the chromatin level in vivo.
Howe ML, Mehmud ZF, Saha S, Buratovich M, Stutius EA, Schmidt HD, Lenon AL, Reddicks C, Ivanov GS, Przyborski SA, Ozer JS
Stem cells and development15(2)175-90(2006 Apr)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Our results provided a new insight into susceptibility of USF1 variant with male infertility. rs11204546 of OR2W3 and rs11677854 of GTF2A1L might be additional valuable molecular predictive markers
Title: Association of single nucleotide polymorphisms in the USF1, GTF2A1L and OR2W3 genes with non-obstructive azoospermia in the Chinese population.
Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.
TFIIA tau is associated with undifferentiated cells during development, yet is down-regulated at the chromatin level upon cellular differentiation.
Title: Transcription Factor IIA tau is associated with undifferentiated cells and its gene expression is repressed in primary neurons at the chromatin level in vivo.
Results identified ALF as a human spermatogenesis related gene, the abnormal expression of ALF might be the partial cause for human infertility.
Title: Involvement of ALF in human spermatogenesis and male infertility.
A unique CpG-specific methylation pattern at the ALF promoter precedes expression in pachytene spermatocytes.
Title: Regulation of ALF gene expression in somatic and male germ line tissues involves partial and site-specific patterns of methylation.
The following GTF2A1L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GTF2A1L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OHu12969![]() |
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Clone ID Related Accession (Same CDS sequence) | NM_006872.4 , NM_006872.5 | |
Accession Version | NM_006872.4 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2017-10-02 | |
Organism | Homo sapiens(human) | |
Product | TFIIA-alpha and beta-like factor isoform 1 | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY049561.1, AC073082.6, BC025991.1 and BG207854.1. On Mar 12, 2014 this sequence version replaced NM_006872.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005643.1, BC025991.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGGCCTGCC TCAACCCGGT GCCTAAACTC TACAGATCTG TAATTGAAGA TGTAATTGAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_006863.2 |
CDS | 96..1532 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGGCCTGCC TCAACCCGGT GCCTAAACTC TACAGATCTG TAATTGAAGA TGTAATTGAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu12969![]() |
|
Clone ID Related Accession (Same CDS sequence) | NM_006872.4 , NM_006872.5 | |
Accession Version | NM_006872.5 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-05-01 | |
Organism | Homo sapiens(human) | |
Product | TFIIA-alpha and beta-like factor isoform 1 | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC025991.1 and BG207854.1. On Nov 22, 2018 this sequence version replaced NM_006872.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK223350.1, SRR5189667.205351.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000403751.8/ ENSP00000384597.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. |
1 | ATGGCCTGCC TCAACCCGGT GCCTAAACTC TACAGATCTG TAATTGAAGA TGTAATTGAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_006863.2 |
CDS | 20..1456 |
Translation |

Target ORF information:
Target ORF information:
|
![]() |

ORF Insert Sequence:
1 | ATGGCCTGCC TCAACCCGGT GCCTAAACTC TACAGATCTG TAATTGAAGA TGTAATTGAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu12972 | |
Clone ID Related Accession (Same CDS sequence) | NM_001193487.2 | |
Accession Version | NM_001193487.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1335bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2018-06-29 | |
Organism | Homo sapiens(human) | |
Product | TFIIA-alpha and beta-like factor isoform 2 | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY049561.1, AK302220.1 and BG207854.1. On Mar 12, 2014 this sequence version replaced NM_001193487.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302220.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGGCCTGCC TCAACCCGGT GCTCTGGGAA ACCAAGGTTT TGCAGTCTAA AGCAACAGAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001180416.1 |
CDS | 96..1430 |
Translation |

Target ORF information:
Target ORF information:
|
![]() |

ORF Insert Sequence:
1 | ATGGCCTGCC TCAACCCGGT GCTCTGGGAA ACCAAGGTTT TGCAGTCTAA AGCAACAGAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
![]() |
Association of single nucleotide polymorphisms in the USF1, GTF2A1L and OR2W3 genes with non-obstructive azoospermia in the Chinese population. |
![]() |
Analysis of copy number variation in Alzheimer's disease in a cohort of clinically characterized and neuropathologically verified individuals. |
![]() |
Genome-wide association study identifies susceptibility loci for polycystic ovary syndrome on chromosome 2p16.3, 2p21 and 9q33.3. |
![]() |
Regulatory factor interactions and somatic silencing of the germ cell-specific ALF gene. |
![]() |
Transcription Factor IIA tau is associated with undifferentiated cells and its gene expression is repressed in primary neurons at the chromatin level in vivo. |