CHRNA4 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following CHRNA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu21241 NM_001256573.2
Latest version!
Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu21796 NM_000744.6
Latest version!
Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu21241 XM_017027625.2
Latest version!
Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu21241 XM_024451822.1
Latest version!
Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu56880 XM_011528524.1
Latest version!
Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu92900 XM_017027624.1
Latest version!
Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $307.30
$439.00
View Old Accession Versions >>
OHu21241 NM_001256573.1 Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu21241 XM_017027625.1 Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu21241
    Clone ID Related Accession (Same CDS sequence) NM_001256573.2 , NM_001256573.1 , XM_017027625.2 , XM_017027625.1 , XM_024451822.1
    Accession Version NM_001256573.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit alpha-4 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62433.1, AL121827.34, BC096292.1, L35901.1, BC096291.1, AK296430.1 and AB209359.1. On Jun 2, 2019 this sequence version replaced NM_001256573.1. Transcript Variant: This variant (2) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC096292.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2145743, SAMEA2146236 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAATTCG GCTCCTGGAC CTACGACAAG GCCAAGATCG ACCTGGTGAA CATGCACAGC 
    CGCGTGGACC AGCTGGACTT CTGGGAGAGT GGCGAGTGGG TCATCGTGGA TGCCGTGGGC
    ACCTACAACA CCAGGAAGTA CGAGTGCTGT GCCGAGATCT ACCCGGACAT CACCTATGCC
    TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
    ATCTCCTGCC TCACCGTGCT GGTCTTCTAC CTGCCCTCCG AGTGTGGCGA GAAGATCACG
    CTGTGCATCT CCGTGCTGCT GTCGCTCACC GTCTTCCTGC TGCTCATCAC CGAGATCATC
    CCGTCCACCT CACTGGTCAT CCCACTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC
    GTCACCCTGT CCATCGTCAT CACGGTCTTC GTGCTCAACG TGCACCACCG CTCGCCACGC
    ACGCACACCA TGCCCACCTG GGTACGCAGG GTCTTCCTGG ACATCGTGCC ACGCCTGCTC
    CTCATGAAGC GGCCGTCCGT GGTCAAGGAC AATTGCCGGC GGCTCATCGA GTCCATGCAT
    AAGATGGCCA GTGCCCCGCG CTTCTGGCCC GAGCCAGAAG GGGAGCCCCC TGCCACGAGC
    GGCACCCAGA GCCTGCACCC GCCCTCACCG TCCTTCTGTG TCCCCCTGGA TGTGCCGGCT
    GAGCCTGGGC CTTCCTGCAA GTCACCCTCC GACCAGCTCC CTCCTCAGCA GCCCCTGGAA
    GCTGAGAAAG CCAGCCCCCA CCCCTCGCCT GGACCCTGCC GCCCGCCCCA CGGCACCCAG
    GCACCAGGGC TGGCCAAAGC CAGGTCCCTC AGCGTCCAGC ACATGTCCAG CCCTGGCGAA
    GCGGTGGAAG GCGGCGTCCG GTGCCGGTCT CGGAGCATCC AGTACTGTGT TCCCCGAGAC
    GATGCCGCCC CCGAGGCAGA TGGCCAGGCT GCCGGCGCCC TGGCCTCTCG CAACACCCAC
    TCGGCTGAGC TCCCACCCCC AGACCAGCCC TCTCCGTGCA AATGCACATG CAAGAAGGAG
    CCCTCTTCGG TGTCCCCGAG CGCCACGGTC AAGACCCGCA GCACCAAAGC GCCGCCCCCG
    CACCTGCCCC TGTCGCCGGC CCTGACCCGG GCGGTGGAGG GCGTCCAGTA CATTGCAGAC
    CACCTGAAGG CCGAAGACAC AGACTTCTCG GTGAAGGAGG ACTGGAAGTA CGTGGCCATG
    GTCATCGACC GCATCTTCCT CTGGATGTTC ATCATCGTCT GCCTGCTGGG GACGGTGGGC
    CTCTTCCTGC CGCCCTGGCT GGCTGGCATG ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001243502.1
    CDS644..1999
    Translation

    Target ORF information:

    RefSeq Version NM_001256573.2
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001256573.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAATTCG GCTCCTGGAC CTACGACAAG GCCAAGATCG ACCTGGTGAA CATGCACAGC 
    CGCGTGGACC AGCTGGACTT CTGGGAGAGT GGCGAGTGGG TCATCGTGGA TGCCGTGGGC
    ACCTACAACA CCAGGAAGTA CGAGTGCTGT GCCGAGATCT ACCCGGACAT CACCTATGCC
    TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
    ATCTCCTGCC TCACCGTGCT GGTCTTCTAC CTGCCCTCCG AGTGTGGCGA GAAGATCACG
    CTGTGCATCT CCGTGCTGCT GTCGCTCACC GTCTTCCTGC TGCTCATCAC CGAGATCATC
    CCGTCCACCT CACTGGTCAT CCCACTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC
    GTCACCCTGT CCATCGTCAT CACGGTCTTC GTGCTCAACG TGCACCACCG CTCGCCACGC
    ACGCACACCA TGCCCACCTG GGTACGCAGG GTCTTCCTGG ACATCGTGCC ACGCCTGCTC
    CTCATGAAGC GGCCGTCCGT GGTCAAGGAC AATTGCCGGC GGCTCATCGA GTCCATGCAT
    AAGATGGCCA GTGCCCCGCG CTTCTGGCCC GAGCCAGAAG GGGAGCCCCC TGCCACGAGC
    GGCACCCAGA GCCTGCACCC GCCCTCACCG TCCTTCTGTG TCCCCCTGGA TGTGCCGGCT
    GAGCCTGGGC CTTCCTGCAA GTCACCCTCC GACCAGCTCC CTCCTCAGCA GCCCCTGGAA
    GCTGAGAAAG CCAGCCCCCA CCCCTCGCCT GGACCCTGCC GCCCGCCCCA CGGCACCCAG
    GCACCAGGGC TGGCCAAAGC CAGGTCCCTC AGCGTCCAGC ACATGTCCAG CCCTGGCGAA
    GCGGTGGAAG GCGGCGTCCG GTGCCGGTCT CGGAGCATCC AGTACTGTGT TCCCCGAGAC
    GATGCCGCCC CCGAGGCAGA TGGCCAGGCT GCCGGCGCCC TGGCCTCTCG CAACACCCAC
    TCGGCTGAGC TCCCACCCCC AGACCAGCCC TCTCCGTGCA AATGCACATG CAAGAAGGAG
    CCCTCTTCGG TGTCCCCGAG CGCCACGGTC AAGACCCGCA GCACCAAAGC GCCGCCCCCG
    CACCTGCCCC TGTCGCCGGC CCTGACCCGG GCGGTGGAGG GCGTCCAGTA CATTGCAGAC
    CACCTGAAGG CCGAAGACAC AGACTTCTCG GTGAAGGAGG ACTGGAAGTA CGTGGCCATG
    GTCATCGACC GCATCTTCCT CTGGATGTTC ATCATCGTCT GCCTGCTGGG GACGGTGGGC
    CTCTTCCTGC CGCCCTGGCT GGCTGGCATG ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21241
    Clone ID Related Accession (Same CDS sequence) NM_001256573.2 , NM_001256573.1 , XM_017027625.2 , XM_017027625.1 , XM_024451822.1
    Accession Version NM_001256573.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit alpha-4 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62433.1, AL121827.34, BC096292.1, L35901.1, BC096291.1, AK296430.1 and AB209359.1. Transcript Variant: This variant (2) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC096292.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2145743, SAMEA2146236 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAATTCG GCTCCTGGAC CTACGACAAG GCCAAGATCG ACCTGGTGAA CATGCACAGC 
    CGCGTGGACC AGCTGGACTT CTGGGAGAGT GGCGAGTGGG TCATCGTGGA TGCCGTGGGC
    ACCTACAACA CCAGGAAGTA CGAGTGCTGT GCCGAGATCT ACCCGGACAT CACCTATGCC
    TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
    ATCTCCTGCC TCACCGTGCT GGTCTTCTAC CTGCCCTCCG AGTGTGGCGA GAAGATCACG
    CTGTGCATCT CCGTGCTGCT GTCGCTCACC GTCTTCCTGC TGCTCATCAC CGAGATCATC
    CCGTCCACCT CACTGGTCAT CCCACTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC
    GTCACCCTGT CCATCGTCAT CACGGTCTTC GTGCTCAACG TGCACCACCG CTCGCCACGC
    ACGCACACCA TGCCCACCTG GGTACGCAGG GTCTTCCTGG ACATCGTGCC ACGCCTGCTC
    CTCATGAAGC GGCCGTCCGT GGTCAAGGAC AATTGCCGGC GGCTCATCGA GTCCATGCAT
    AAGATGGCCA GTGCCCCGCG CTTCTGGCCC GAGCCAGAAG GGGAGCCCCC TGCCACGAGC
    GGCACCCAGA GCCTGCACCC GCCCTCACCG TCCTTCTGTG TCCCCCTGGA TGTGCCGGCT
    GAGCCTGGGC CTTCCTGCAA GTCACCCTCC GACCAGCTCC CTCCTCAGCA GCCCCTGGAA
    GCTGAGAAAG CCAGCCCCCA CCCCTCGCCT GGACCCTGCC GCCCGCCCCA CGGCACCCAG
    GCACCAGGGC TGGCCAAAGC CAGGTCCCTC AGCGTCCAGC ACATGTCCAG CCCTGGCGAA
    GCGGTGGAAG GCGGCGTCCG GTGCCGGTCT CGGAGCATCC AGTACTGTGT TCCCCGAGAC
    GATGCCGCCC CCGAGGCAGA TGGCCAGGCT GCCGGCGCCC TGGCCTCTCG CAACACCCAC
    TCGGCTGAGC TCCCACCCCC AGACCAGCCC TCTCCGTGCA AATGCACATG CAAGAAGGAG
    CCCTCTTCGG TGTCCCCGAG CGCCACGGTC AAGACCCGCA GCACCAAAGC GCCGCCCCCG
    CACCTGCCCC TGTCGCCGGC CCTGACCCGG GCGGTGGAGG GCGTCCAGTA CATTGCAGAC
    CACCTGAAGG CCGAAGACAC AGACTTCTCG GTGAAGGAGG ACTGGAAGTA CGTGGCCATG
    GTCATCGACC GCATCTTCCT CTGGATGTTC ATCATCGTCT GCCTGCTGGG GACGGTGGGC
    CTCTTCCTGC CGCCCTGGCT GGCTGGCATG ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001243502.1
    CDS691..2046
    Translation

    Target ORF information:

    RefSeq Version NM_001256573.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001256573.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAATTCG GCTCCTGGAC CTACGACAAG GCCAAGATCG ACCTGGTGAA CATGCACAGC 
    CGCGTGGACC AGCTGGACTT CTGGGAGAGT GGCGAGTGGG TCATCGTGGA TGCCGTGGGC
    ACCTACAACA CCAGGAAGTA CGAGTGCTGT GCCGAGATCT ACCCGGACAT CACCTATGCC
    TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
    ATCTCCTGCC TCACCGTGCT GGTCTTCTAC CTGCCCTCCG AGTGTGGCGA GAAGATCACG
    CTGTGCATCT CCGTGCTGCT GTCGCTCACC GTCTTCCTGC TGCTCATCAC CGAGATCATC
    CCGTCCACCT CACTGGTCAT CCCACTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC
    GTCACCCTGT CCATCGTCAT CACGGTCTTC GTGCTCAACG TGCACCACCG CTCGCCACGC
    ACGCACACCA TGCCCACCTG GGTACGCAGG GTCTTCCTGG ACATCGTGCC ACGCCTGCTC
    CTCATGAAGC GGCCGTCCGT GGTCAAGGAC AATTGCCGGC GGCTCATCGA GTCCATGCAT
    AAGATGGCCA GTGCCCCGCG CTTCTGGCCC GAGCCAGAAG GGGAGCCCCC TGCCACGAGC
    GGCACCCAGA GCCTGCACCC GCCCTCACCG TCCTTCTGTG TCCCCCTGGA TGTGCCGGCT
    GAGCCTGGGC CTTCCTGCAA GTCACCCTCC GACCAGCTCC CTCCTCAGCA GCCCCTGGAA
    GCTGAGAAAG CCAGCCCCCA CCCCTCGCCT GGACCCTGCC GCCCGCCCCA CGGCACCCAG
    GCACCAGGGC TGGCCAAAGC CAGGTCCCTC AGCGTCCAGC ACATGTCCAG CCCTGGCGAA
    GCGGTGGAAG GCGGCGTCCG GTGCCGGTCT CGGAGCATCC AGTACTGTGT TCCCCGAGAC
    GATGCCGCCC CCGAGGCAGA TGGCCAGGCT GCCGGCGCCC TGGCCTCTCG CAACACCCAC
    TCGGCTGAGC TCCCACCCCC AGACCAGCCC TCTCCGTGCA AATGCACATG CAAGAAGGAG
    CCCTCTTCGG TGTCCCCGAG CGCCACGGTC AAGACCCGCA GCACCAAAGC GCCGCCCCCG
    CACCTGCCCC TGTCGCCGGC CCTGACCCGG GCGGTGGAGG GCGTCCAGTA CATTGCAGAC
    CACCTGAAGG CCGAAGACAC AGACTTCTCG GTGAAGGAGG ACTGGAAGTA CGTGGCCATG
    GTCATCGACC GCATCTTCCT CTGGATGTTC ATCATCGTCT GCCTGCTGGG GACGGTGGGC
    CTCTTCCTGC CGCCCTGGCT GGCTGGCATG ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21796
    Clone ID Related Accession (Same CDS sequence) NM_000744.6
    Accession Version NM_000744.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1884bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit alpha-4 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62433.1, AL121827.34, L35901.1, BC096291.1, AK296430.1 and AB209359.1. This sequence is a reference standard in the RefSeqGene project. On Feb 10, 2012 this sequence version replaced NM_000744.5. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U62433.1, BC096290.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein regulatory uORF :: PMID: 23843950 ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAGCTAG GGGGCCCCGG AGCGCCGCGG CTGCTGCCGC CGCTGCTGCT GCTTCTGGGG 
    ACCGGCCTCC TGCGCGCCAG CAGCCATGTG GAGACCCGGG CCCACGCCGA GGAGCGGCTC
    CTGAAGAAAC TCTTCTCCGG TTACAACAAG TGGTCCCGAC CCGTGGCCAA CATCTCGGAC
    GTGGTCCTCG TCCGCTTCGG CCTGTCCATC GCTCAGCTCA TTGACGTGGA TGAGAAGAAC
    CAGATGATGA CCACGAACGT ATGGGTGAAG CAGGAGTGGC ACGACTACAA GCTGCGCTGG
    GACCCAGCTG ACTATGAGAA TGTCACCTCC ATCCGCATCC CCTCCGAGCT CATCTGGCGG
    CCGGACATCG TCCTCTACAA CAATGCTGAC GGGGACTTCG CGGTCACCCA CCTGACCAAG
    GCCCACCTGT TCCATGACGG GCGGGTGCAG TGGACTCCCC CGGCCATTTA CAAGAGCTCC
    TGCAGCATCG ACGTCACCTT CTTCCCCTTC GACCAGCAGA ACTGCACCAT GAAATTCGGC
    TCCTGGACCT ACGACAAGGC CAAGATCGAC CTGGTGAACA TGCACAGCCG CGTGGACCAG
    CTGGACTTCT GGGAGAGTGG CGAGTGGGTC ATCGTGGATG CCGTGGGCAC CTACAACACC
    AGGAAGTACG AGTGCTGTGC CGAGATCTAC CCGGACATCA CCTATGCCTT CGTCATCCGG
    CGGCTGCCGC TCTTCTACAC CATCAACCTC ATCATCCCCT GCCTGCTCAT CTCCTGCCTC
    ACCGTGCTGG TCTTCTACCT GCCCTCCGAG TGTGGCGAGA AGATCACGCT GTGCATCTCC
    GTGCTGCTGT CGCTCACCGT CTTCCTGCTG CTCATCACCG AGATCATCCC GTCCACCTCA
    CTGGTCATCC CACTCATCGG CGAGTACCTG CTGTTCACCA TGATCTTCGT CACCCTGTCC
    ATCGTCATCA CGGTCTTCGT GCTCAACGTG CACCACCGCT CGCCACGCAC GCACACCATG
    CCCACCTGGG TACGCAGGGT CTTCCTGGAC ATCGTGCCAC GCCTGCTCCT CATGAAGCGG
    CCGTCCGTGG TCAAGGACAA TTGCCGGCGG CTCATCGAGT CCATGCATAA GATGGCCAGT
    GCCCCGCGCT TCTGGCCCGA GCCAGAAGGG GAGCCCCCTG CCACGAGCGG CACCCAGAGC
    CTGCACCCGC CCTCACCGTC CTTCTGTGTC CCCCTGGATG TGCCGGCTGA GCCTGGGCCT
    TCCTGCAAGT CACCCTCCGA CCAGCTCCCT CCTCAGCAGC CCCTGGAAGC TGAGAAAGCC
    AGCCCCCACC CCTCGCCTGG ACCCTGCCGC CCGCCCCACG GCACCCAGGC ACCAGGGCTG
    GCCAAAGCCA GGTCCCTCAG CGTCCAGCAC ATGTCCAGCC CTGGCGAAGC GGTGGAAGGC
    GGCGTCCGGT GCCGGTCTCG GAGCATCCAG TACTGTGTTC CCCGAGACGA TGCCGCCCCC
    GAGGCAGATG GCCAGGCTGC CGGCGCCCTG GCCTCTCGCA ACACCCACTC GGCTGAGCTC
    CCACCCCCAG ACCAGCCCTC TCCGTGCAAA TGCACATGCA AGAAGGAGCC CTCTTCGGTG
    TCCCCGAGCG CCACGGTCAA GACCCGCAGC ACCAAAGCGC CGCCCCCGCA CCTGCCCCTG
    TCGCCGGCCC TGACCCGGGC GGTGGAGGGC GTCCAGTACA TTGCAGACCA CCTGAAGGCC
    GAAGACACAG ACTTCTCGGT GAAGGAGGAC TGGAAGTACG TGGCCATGGT CATCGACCGC
    ATCTTCCTCT GGATGTTCAT CATCGTCTGC CTGCTGGGGA CGGTGGGCCT CTTCCTGCCG
    CCCTGGCTGG CTGGCATGAT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000735.1
    CDS232..2115
    Translation

    Target ORF information:

    RefSeq Version NM_000744.6
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000744.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAGCTAG GGGGCCCCGG AGCGCCGCGG CTGCTGCCGC CGCTGCTGCT GCTTCTGGGG 
    ACCGGCCTCC TGCGCGCCAG CAGCCATGTG GAGACCCGGG CCCACGCCGA GGAGCGGCTC
    CTGAAGAAAC TCTTCTCCGG TTACAACAAG TGGTCCCGAC CCGTGGCCAA CATCTCGGAC
    GTGGTCCTCG TCCGCTTCGG CCTGTCCATC GCTCAGCTCA TTGACGTGGA TGAGAAGAAC
    CAGATGATGA CCACGAACGT ATGGGTGAAG CAGGAGTGGC ACGACTACAA GCTGCGCTGG
    GACCCAGCTG ACTATGAGAA TGTCACCTCC ATCCGCATCC CCTCCGAGCT CATCTGGCGG
    CCGGACATCG TCCTCTACAA CAATGCTGAC GGGGACTTCG CGGTCACCCA CCTGACCAAG
    GCCCACCTGT TCCATGACGG GCGGGTGCAG TGGACTCCCC CGGCCATTTA CAAGAGCTCC
    TGCAGCATCG ACGTCACCTT CTTCCCCTTC GACCAGCAGA ACTGCACCAT GAAATTCGGC
    TCCTGGACCT ACGACAAGGC CAAGATCGAC CTGGTGAACA TGCACAGCCG CGTGGACCAG
    CTGGACTTCT GGGAGAGTGG CGAGTGGGTC ATCGTGGATG CCGTGGGCAC CTACAACACC
    AGGAAGTACG AGTGCTGTGC CGAGATCTAC CCGGACATCA CCTATGCCTT CGTCATCCGG
    CGGCTGCCGC TCTTCTACAC CATCAACCTC ATCATCCCCT GCCTGCTCAT CTCCTGCCTC
    ACCGTGCTGG TCTTCTACCT GCCCTCCGAG TGTGGCGAGA AGATCACGCT GTGCATCTCC
    GTGCTGCTGT CGCTCACCGT CTTCCTGCTG CTCATCACCG AGATCATCCC GTCCACCTCA
    CTGGTCATCC CACTCATCGG CGAGTACCTG CTGTTCACCA TGATCTTCGT CACCCTGTCC
    ATCGTCATCA CGGTCTTCGT GCTCAACGTG CACCACCGCT CGCCACGCAC GCACACCATG
    CCCACCTGGG TACGCAGGGT CTTCCTGGAC ATCGTGCCAC GCCTGCTCCT CATGAAGCGG
    CCGTCCGTGG TCAAGGACAA TTGCCGGCGG CTCATCGAGT CCATGCATAA GATGGCCAGT
    GCCCCGCGCT TCTGGCCCGA GCCAGAAGGG GAGCCCCCTG CCACGAGCGG CACCCAGAGC
    CTGCACCCGC CCTCACCGTC CTTCTGTGTC CCCCTGGATG TGCCGGCTGA GCCTGGGCCT
    TCCTGCAAGT CACCCTCCGA CCAGCTCCCT CCTCAGCAGC CCCTGGAAGC TGAGAAAGCC
    AGCCCCCACC CCTCGCCTGG ACCCTGCCGC CCGCCCCACG GCACCCAGGC ACCAGGGCTG
    GCCAAAGCCA GGTCCCTCAG CGTCCAGCAC ATGTCCAGCC CTGGCGAAGC GGTGGAAGGC
    GGCGTCCGGT GCCGGTCTCG GAGCATCCAG TACTGTGTTC CCCGAGACGA TGCCGCCCCC
    GAGGCAGATG GCCAGGCTGC CGGCGCCCTG GCCTCTCGCA ACACCCACTC GGCTGAGCTC
    CCACCCCCAG ACCAGCCCTC TCCGTGCAAA TGCACATGCA AGAAGGAGCC CTCTTCGGTG
    TCCCCGAGCG CCACGGTCAA GACCCGCAGC ACCAAAGCGC CGCCCCCGCA CCTGCCCCTG
    TCGCCGGCCC TGACCCGGGC GGTGGAGGGC GTCCAGTACA TTGCAGACCA CCTGAAGGCC
    GAAGACACAG ACTTCTCGGT GAAGGAGGAC TGGAAGTACG TGGCCATGGT CATCGACCGC
    ATCTTCCTCT GGATGTTCAT CATCGTCTGC CTGCTGGGGA CGGTGGGCCT CTTCCTGCCG
    CCCTGGCTGG CTGGCATGAT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21241
    Clone ID Related Accession (Same CDS sequence) NM_001256573.2 , NM_001256573.1 , XM_017027625.2 , XM_017027625.1 , XM_024451822.1
    Accession Version XM_017027625.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit alpha-4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017027625.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAATTCG GCTCCTGGAC CTACGACAAG GCCAAGATCG ACCTGGTGAA CATGCACAGC 
    CGCGTGGACC AGCTGGACTT CTGGGAGAGT GGCGAGTGGG TCATCGTGGA TGCCGTGGGC
    ACCTACAACA CCAGGAAGTA CGAGTGCTGT GCCGAGATCT ACCCGGACAT CACCTATGCC
    TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
    ATCTCCTGCC TCACCGTGCT GGTCTTCTAC CTGCCCTCCG AGTGTGGCGA GAAGATCACG
    CTGTGCATCT CCGTGCTGCT GTCGCTCACC GTCTTCCTGC TGCTCATCAC CGAGATCATC
    CCGTCCACCT CACTGGTCAT CCCACTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC
    GTCACCCTGT CCATCGTCAT CACGGTCTTC GTGCTCAACG TGCACCACCG CTCGCCACGC
    ACGCACACCA TGCCCACCTG GGTACGCAGG GTCTTCCTGG ACATCGTGCC ACGCCTGCTC
    CTCATGAAGC GGCCGTCCGT GGTCAAGGAC AATTGCCGGC GGCTCATCGA GTCCATGCAT
    AAGATGGCCA GTGCCCCGCG CTTCTGGCCC GAGCCAGAAG GGGAGCCCCC TGCCACGAGC
    GGCACCCAGA GCCTGCACCC GCCCTCACCG TCCTTCTGTG TCCCCCTGGA TGTGCCGGCT
    GAGCCTGGGC CTTCCTGCAA GTCACCCTCC GACCAGCTCC CTCCTCAGCA GCCCCTGGAA
    GCTGAGAAAG CCAGCCCCCA CCCCTCGCCT GGACCCTGCC GCCCGCCCCA CGGCACCCAG
    GCACCAGGGC TGGCCAAAGC CAGGTCCCTC AGCGTCCAGC ACATGTCCAG CCCTGGCGAA
    GCGGTGGAAG GCGGCGTCCG GTGCCGGTCT CGGAGCATCC AGTACTGTGT TCCCCGAGAC
    GATGCCGCCC CCGAGGCAGA TGGCCAGGCT GCCGGCGCCC TGGCCTCTCG CAACACCCAC
    TCGGCTGAGC TCCCACCCCC AGACCAGCCC TCTCCGTGCA AATGCACATG CAAGAAGGAG
    CCCTCTTCGG TGTCCCCGAG CGCCACGGTC AAGACCCGCA GCACCAAAGC GCCGCCCCCG
    CACCTGCCCC TGTCGCCGGC CCTGACCCGG GCGGTGGAGG GCGTCCAGTA CATTGCAGAC
    CACCTGAAGG CCGAAGACAC AGACTTCTCG GTGAAGGAGG ACTGGAAGTA CGTGGCCATG
    GTCATCGACC GCATCTTCCT CTGGATGTTC ATCATCGTCT GCCTGCTGGG GACGGTGGGC
    CTCTTCCTGC CGCCCTGGCT GGCTGGCATG ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016883114.1
    CDS889..2244
    Translation

    Target ORF information:

    RefSeq Version XM_017027625.2
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017027625.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAATTCG GCTCCTGGAC CTACGACAAG GCCAAGATCG ACCTGGTGAA CATGCACAGC 
    CGCGTGGACC AGCTGGACTT CTGGGAGAGT GGCGAGTGGG TCATCGTGGA TGCCGTGGGC
    ACCTACAACA CCAGGAAGTA CGAGTGCTGT GCCGAGATCT ACCCGGACAT CACCTATGCC
    TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
    ATCTCCTGCC TCACCGTGCT GGTCTTCTAC CTGCCCTCCG AGTGTGGCGA GAAGATCACG
    CTGTGCATCT CCGTGCTGCT GTCGCTCACC GTCTTCCTGC TGCTCATCAC CGAGATCATC
    CCGTCCACCT CACTGGTCAT CCCACTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC
    GTCACCCTGT CCATCGTCAT CACGGTCTTC GTGCTCAACG TGCACCACCG CTCGCCACGC
    ACGCACACCA TGCCCACCTG GGTACGCAGG GTCTTCCTGG ACATCGTGCC ACGCCTGCTC
    CTCATGAAGC GGCCGTCCGT GGTCAAGGAC AATTGCCGGC GGCTCATCGA GTCCATGCAT
    AAGATGGCCA GTGCCCCGCG CTTCTGGCCC GAGCCAGAAG GGGAGCCCCC TGCCACGAGC
    GGCACCCAGA GCCTGCACCC GCCCTCACCG TCCTTCTGTG TCCCCCTGGA TGTGCCGGCT
    GAGCCTGGGC CTTCCTGCAA GTCACCCTCC GACCAGCTCC CTCCTCAGCA GCCCCTGGAA
    GCTGAGAAAG CCAGCCCCCA CCCCTCGCCT GGACCCTGCC GCCCGCCCCA CGGCACCCAG
    GCACCAGGGC TGGCCAAAGC CAGGTCCCTC AGCGTCCAGC ACATGTCCAG CCCTGGCGAA
    GCGGTGGAAG GCGGCGTCCG GTGCCGGTCT CGGAGCATCC AGTACTGTGT TCCCCGAGAC
    GATGCCGCCC CCGAGGCAGA TGGCCAGGCT GCCGGCGCCC TGGCCTCTCG CAACACCCAC
    TCGGCTGAGC TCCCACCCCC AGACCAGCCC TCTCCGTGCA AATGCACATG CAAGAAGGAG
    CCCTCTTCGG TGTCCCCGAG CGCCACGGTC AAGACCCGCA GCACCAAAGC GCCGCCCCCG
    CACCTGCCCC TGTCGCCGGC CCTGACCCGG GCGGTGGAGG GCGTCCAGTA CATTGCAGAC
    CACCTGAAGG CCGAAGACAC AGACTTCTCG GTGAAGGAGG ACTGGAAGTA CGTGGCCATG
    GTCATCGACC GCATCTTCCT CTGGATGTTC ATCATCGTCT GCCTGCTGGG GACGGTGGGC
    CTCTTCCTGC CGCCCTGGCT GGCTGGCATG ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21241
    Clone ID Related Accession (Same CDS sequence) NM_001256573.2 , NM_001256573.1 , XM_017027625.2 , XM_017027625.1 , XM_024451822.1
    Accession Version XM_017027625.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit alpha-4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAATTCG GCTCCTGGAC CTACGACAAG GCCAAGATCG ACCTGGTGAA CATGCACAGC 
    CGCGTGGACC AGCTGGACTT CTGGGAGAGT GGCGAGTGGG TCATCGTGGA TGCCGTGGGC
    ACCTACAACA CCAGGAAGTA CGAGTGCTGT GCCGAGATCT ACCCGGACAT CACCTATGCC
    TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
    ATCTCCTGCC TCACCGTGCT GGTCTTCTAC CTGCCCTCCG AGTGTGGCGA GAAGATCACG
    CTGTGCATCT CCGTGCTGCT GTCGCTCACC GTCTTCCTGC TGCTCATCAC CGAGATCATC
    CCGTCCACCT CACTGGTCAT CCCACTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC
    GTCACCCTGT CCATCGTCAT CACGGTCTTC GTGCTCAACG TGCACCACCG CTCGCCACGC
    ACGCACACCA TGCCCACCTG GGTACGCAGG GTCTTCCTGG ACATCGTGCC ACGCCTGCTC
    CTCATGAAGC GGCCGTCCGT GGTCAAGGAC AATTGCCGGC GGCTCATCGA GTCCATGCAT
    AAGATGGCCA GTGCCCCGCG CTTCTGGCCC GAGCCAGAAG GGGAGCCCCC TGCCACGAGC
    GGCACCCAGA GCCTGCACCC GCCCTCACCG TCCTTCTGTG TCCCCCTGGA TGTGCCGGCT
    GAGCCTGGGC CTTCCTGCAA GTCACCCTCC GACCAGCTCC CTCCTCAGCA GCCCCTGGAA
    GCTGAGAAAG CCAGCCCCCA CCCCTCGCCT GGACCCTGCC GCCCGCCCCA CGGCACCCAG
    GCACCAGGGC TGGCCAAAGC CAGGTCCCTC AGCGTCCAGC ACATGTCCAG CCCTGGCGAA
    GCGGTGGAAG GCGGCGTCCG GTGCCGGTCT CGGAGCATCC AGTACTGTGT TCCCCGAGAC
    GATGCCGCCC CCGAGGCAGA TGGCCAGGCT GCCGGCGCCC TGGCCTCTCG CAACACCCAC
    TCGGCTGAGC TCCCACCCCC AGACCAGCCC TCTCCGTGCA AATGCACATG CAAGAAGGAG
    CCCTCTTCGG TGTCCCCGAG CGCCACGGTC AAGACCCGCA GCACCAAAGC GCCGCCCCCG
    CACCTGCCCC TGTCGCCGGC CCTGACCCGG GCGGTGGAGG GCGTCCAGTA CATTGCAGAC
    CACCTGAAGG CCGAAGACAC AGACTTCTCG GTGAAGGAGG ACTGGAAGTA CGTGGCCATG
    GTCATCGACC GCATCTTCCT CTGGATGTTC ATCATCGTCT GCCTGCTGGG GACGGTGGGC
    CTCTTCCTGC CGCCCTGGCT GGCTGGCATG ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016883114.1
    CDS810..2165
    Translation

    Target ORF information:

    RefSeq Version XM_017027625.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017027625.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAATTCG GCTCCTGGAC CTACGACAAG GCCAAGATCG ACCTGGTGAA CATGCACAGC 
    CGCGTGGACC AGCTGGACTT CTGGGAGAGT GGCGAGTGGG TCATCGTGGA TGCCGTGGGC
    ACCTACAACA CCAGGAAGTA CGAGTGCTGT GCCGAGATCT ACCCGGACAT CACCTATGCC
    TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
    ATCTCCTGCC TCACCGTGCT GGTCTTCTAC CTGCCCTCCG AGTGTGGCGA GAAGATCACG
    CTGTGCATCT CCGTGCTGCT GTCGCTCACC GTCTTCCTGC TGCTCATCAC CGAGATCATC
    CCGTCCACCT CACTGGTCAT CCCACTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC
    GTCACCCTGT CCATCGTCAT CACGGTCTTC GTGCTCAACG TGCACCACCG CTCGCCACGC
    ACGCACACCA TGCCCACCTG GGTACGCAGG GTCTTCCTGG ACATCGTGCC ACGCCTGCTC
    CTCATGAAGC GGCCGTCCGT GGTCAAGGAC AATTGCCGGC GGCTCATCGA GTCCATGCAT
    AAGATGGCCA GTGCCCCGCG CTTCTGGCCC GAGCCAGAAG GGGAGCCCCC TGCCACGAGC
    GGCACCCAGA GCCTGCACCC GCCCTCACCG TCCTTCTGTG TCCCCCTGGA TGTGCCGGCT
    GAGCCTGGGC CTTCCTGCAA GTCACCCTCC GACCAGCTCC CTCCTCAGCA GCCCCTGGAA
    GCTGAGAAAG CCAGCCCCCA CCCCTCGCCT GGACCCTGCC GCCCGCCCCA CGGCACCCAG
    GCACCAGGGC TGGCCAAAGC CAGGTCCCTC AGCGTCCAGC ACATGTCCAG CCCTGGCGAA
    GCGGTGGAAG GCGGCGTCCG GTGCCGGTCT CGGAGCATCC AGTACTGTGT TCCCCGAGAC
    GATGCCGCCC CCGAGGCAGA TGGCCAGGCT GCCGGCGCCC TGGCCTCTCG CAACACCCAC
    TCGGCTGAGC TCCCACCCCC AGACCAGCCC TCTCCGTGCA AATGCACATG CAAGAAGGAG
    CCCTCTTCGG TGTCCCCGAG CGCCACGGTC AAGACCCGCA GCACCAAAGC GCCGCCCCCG
    CACCTGCCCC TGTCGCCGGC CCTGACCCGG GCGGTGGAGG GCGTCCAGTA CATTGCAGAC
    CACCTGAAGG CCGAAGACAC AGACTTCTCG GTGAAGGAGG ACTGGAAGTA CGTGGCCATG
    GTCATCGACC GCATCTTCCT CTGGATGTTC ATCATCGTCT GCCTGCTGGG GACGGTGGGC
    CTCTTCCTGC CGCCCTGGCT GGCTGGCATG ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21241
    Clone ID Related Accession (Same CDS sequence) NM_001256573.2 , NM_001256573.1 , XM_017027625.2 , XM_017027625.1 , XM_024451822.1
    Accession Version XM_024451822.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit alpha-4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAATTCG GCTCCTGGAC CTACGACAAG GCCAAGATCG ACCTGGTGAA CATGCACAGC 
    CGCGTGGACC AGCTGGACTT CTGGGAGAGT GGCGAGTGGG TCATCGTGGA TGCCGTGGGC
    ACCTACAACA CCAGGAAGTA CGAGTGCTGT GCCGAGATCT ACCCGGACAT CACCTATGCC
    TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
    ATCTCCTGCC TCACCGTGCT GGTCTTCTAC CTGCCCTCCG AGTGTGGCGA GAAGATCACG
    CTGTGCATCT CCGTGCTGCT GTCGCTCACC GTCTTCCTGC TGCTCATCAC CGAGATCATC
    CCGTCCACCT CACTGGTCAT CCCACTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC
    GTCACCCTGT CCATCGTCAT CACGGTCTTC GTGCTCAACG TGCACCACCG CTCGCCACGC
    ACGCACACCA TGCCCACCTG GGTACGCAGG GTCTTCCTGG ACATCGTGCC ACGCCTGCTC
    CTCATGAAGC GGCCGTCCGT GGTCAAGGAC AATTGCCGGC GGCTCATCGA GTCCATGCAT
    AAGATGGCCA GTGCCCCGCG CTTCTGGCCC GAGCCAGAAG GGGAGCCCCC TGCCACGAGC
    GGCACCCAGA GCCTGCACCC GCCCTCACCG TCCTTCTGTG TCCCCCTGGA TGTGCCGGCT
    GAGCCTGGGC CTTCCTGCAA GTCACCCTCC GACCAGCTCC CTCCTCAGCA GCCCCTGGAA
    GCTGAGAAAG CCAGCCCCCA CCCCTCGCCT GGACCCTGCC GCCCGCCCCA CGGCACCCAG
    GCACCAGGGC TGGCCAAAGC CAGGTCCCTC AGCGTCCAGC ACATGTCCAG CCCTGGCGAA
    GCGGTGGAAG GCGGCGTCCG GTGCCGGTCT CGGAGCATCC AGTACTGTGT TCCCCGAGAC
    GATGCCGCCC CCGAGGCAGA TGGCCAGGCT GCCGGCGCCC TGGCCTCTCG CAACACCCAC
    TCGGCTGAGC TCCCACCCCC AGACCAGCCC TCTCCGTGCA AATGCACATG CAAGAAGGAG
    CCCTCTTCGG TGTCCCCGAG CGCCACGGTC AAGACCCGCA GCACCAAAGC GCCGCCCCCG
    CACCTGCCCC TGTCGCCGGC CCTGACCCGG GCGGTGGAGG GCGTCCAGTA CATTGCAGAC
    CACCTGAAGG CCGAAGACAC AGACTTCTCG GTGAAGGAGG ACTGGAAGTA CGTGGCCATG
    GTCATCGACC GCATCTTCCT CTGGATGTTC ATCATCGTCT GCCTGCTGGG GACGGTGGGC
    CTCTTCCTGC CGCCCTGGCT GGCTGGCATG ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024307590.1
    CDS234..1589
    Translation

    Target ORF information:

    RefSeq Version XM_024451822.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024451822.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAATTCG GCTCCTGGAC CTACGACAAG GCCAAGATCG ACCTGGTGAA CATGCACAGC 
    CGCGTGGACC AGCTGGACTT CTGGGAGAGT GGCGAGTGGG TCATCGTGGA TGCCGTGGGC
    ACCTACAACA CCAGGAAGTA CGAGTGCTGT GCCGAGATCT ACCCGGACAT CACCTATGCC
    TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
    ATCTCCTGCC TCACCGTGCT GGTCTTCTAC CTGCCCTCCG AGTGTGGCGA GAAGATCACG
    CTGTGCATCT CCGTGCTGCT GTCGCTCACC GTCTTCCTGC TGCTCATCAC CGAGATCATC
    CCGTCCACCT CACTGGTCAT CCCACTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC
    GTCACCCTGT CCATCGTCAT CACGGTCTTC GTGCTCAACG TGCACCACCG CTCGCCACGC
    ACGCACACCA TGCCCACCTG GGTACGCAGG GTCTTCCTGG ACATCGTGCC ACGCCTGCTC
    CTCATGAAGC GGCCGTCCGT GGTCAAGGAC AATTGCCGGC GGCTCATCGA GTCCATGCAT
    AAGATGGCCA GTGCCCCGCG CTTCTGGCCC GAGCCAGAAG GGGAGCCCCC TGCCACGAGC
    GGCACCCAGA GCCTGCACCC GCCCTCACCG TCCTTCTGTG TCCCCCTGGA TGTGCCGGCT
    GAGCCTGGGC CTTCCTGCAA GTCACCCTCC GACCAGCTCC CTCCTCAGCA GCCCCTGGAA
    GCTGAGAAAG CCAGCCCCCA CCCCTCGCCT GGACCCTGCC GCCCGCCCCA CGGCACCCAG
    GCACCAGGGC TGGCCAAAGC CAGGTCCCTC AGCGTCCAGC ACATGTCCAG CCCTGGCGAA
    GCGGTGGAAG GCGGCGTCCG GTGCCGGTCT CGGAGCATCC AGTACTGTGT TCCCCGAGAC
    GATGCCGCCC CCGAGGCAGA TGGCCAGGCT GCCGGCGCCC TGGCCTCTCG CAACACCCAC
    TCGGCTGAGC TCCCACCCCC AGACCAGCCC TCTCCGTGCA AATGCACATG CAAGAAGGAG
    CCCTCTTCGG TGTCCCCGAG CGCCACGGTC AAGACCCGCA GCACCAAAGC GCCGCCCCCG
    CACCTGCCCC TGTCGCCGGC CCTGACCCGG GCGGTGGAGG GCGTCCAGTA CATTGCAGAC
    CACCTGAAGG CCGAAGACAC AGACTTCTCG GTGAAGGAGG ACTGGAAGTA CGTGGCCATG
    GTCATCGACC GCATCTTCCT CTGGATGTTC ATCATCGTCT GCCTGCTGGG GACGGTGGGC
    CTCTTCCTGC CGCCCTGGCT GGCTGGCATG ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56880
    Clone ID Related Accession (Same CDS sequence) XM_011528524.1
    Accession Version XM_011528524.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1671bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit alpha-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAGAATGT CACCTCCATC CGCATCCCCT CCGAGCTCAT CTGGCGGCCG GACATCGTCC 
    TCTACAACAA CTGCAGGAGA GCCCTTTTGG GCAGGAGTGC TTTTCGCCAT CAGACCCCAT
    CCTGGCCTTT CTGGGAGAAT AGTGTGGACT GCAGGGTGCC CTGGAGAGGG TGCTGACGGG
    GACTTCGCGG TCACCCACCT GACCAAGGCC CACCTGTTCC ATGACGGGCG GGTGCAGTGG
    ACTCCCCCGG CCATTTACAA GAGCTCCTGC AGCATCGACG TCACCTTCTT CCCCTTCGAC
    CAGCAGAACT GCACCATGAA ATTCGGCTCC TGGACCTACG ACAAGGCCAA GATCGACCTG
    GTGAACATGC ACAGCCGCGT GGACCAGCTG GACTTCTGGG AGAGTGGCGA GTGGGTCATC
    GTGGATGCCG TGGGCACCTA CAACACCAGG AAGTACGAGT GCTGTGCCGA GATCTACCCG
    GACATCACCT ATGCCTTCGT CATCCGGCGG CTGCCGCTCT TCTACACCAT CAACCTCATC
    ATCCCCTGCC TGCTCATCTC CTGCCTCACC GTGCTGGTCT TCTACCTGCC CTCCGAGTGT
    GGCGAGAAGA TCACGCTGTG CATCTCCGTG CTGCTGTCGC TCACCGTCTT CCTGCTGCTC
    ATCACCGAGA TCATCCCGTC CACCTCACTG GTCATCCCAC TCATCGGCGA GTACCTGCTG
    TTCACCATGA TCTTCGTCAC CCTGTCCATC GTCATCACGG TCTTCGTGCT CAACGTGCAC
    CACCGCTCGC CACGCACGCA CACCATGCCC ACCTGGGTAC GCAGGGTCTT CCTGGACATC
    GTGCCACGCC TGCTCCTCAT GAAGCGGCCG TCCGTGGTCA AGGACAATTG CCGGCGGCTC
    ATCGAGTCCA TGCATAAGAT GGCCAGTGCC CCGCGCTTCT GGCCCGAGCC AGAAGGGGAG
    CCCCCTGCCA CGAGCGGCAC CCAGAGCCTG CACCCGCCCT CACCGTCCTT CTGTGTCCCC
    CTGGATGTGC CGGCTGAGCC TGGGCCTTCC TGCAAGTCAC CCTCCGACCA GCTCCCTCCT
    CAGCAGCCCC TGGAAGCTGA GAAAGCCAGC CCCCACCCCT CGCCTGGACC CTGCCGCCCG
    CCCCACGGCA CCCAGGCACC AGGGCTGGCC AAAGCCAGGT CCCTCAGCGT CCAGCACATG
    TCCAGCCCTG GCGAAGCGGT GGAAGGCGGC GTCCGGTGCC GGTCTCGGAG CATCCAGTAC
    TGTGTTCCCC GAGACGATGC CGCCCCCGAG GCAGATGGCC AGGCTGCCGG CGCCCTGGCC
    TCTCGCAACA CCCACTCGGC TGAGCTCCCA CCCCCAGACC AGCCCTCTCC GTGCAAATGC
    ACATGCAAGA AGGAGCCCTC TTCGGTGTCC CCGAGCGCCA CGGTCAAGAC CCGCAGCACC
    AAAGCGCCGC CCCCGCACCT GCCCCTGTCG CCGGCCCTGA CCCGGGCGGT GGAGGGCGTC
    CAGTACATTG CAGACCACCT GAAGGCCGAA GACACAGACT TCTCGGTGAA GGAGGACTGG
    AAGTACGTGG CCATGGTCAT CGACCGCATC TTCCTCTGGA TGTTCATCAT CGTCTGCCTG
    CTGGGGACGG TGGGCCTCTT CCTGCCGCCC TGGCTGGCTG GCATGATCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011526826.1
    CDS89..1759
    Translation

    Target ORF information:

    RefSeq Version XM_011528524.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011528524.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAGAATGT CACCTCCATC CGCATCCCCT CCGAGCTCAT CTGGCGGCCG GACATCGTCC 
    TCTACAACAA CTGCAGGAGA GCCCTTTTGG GCAGGAGTGC TTTTCGCCAT CAGACCCCAT
    CCTGGCCTTT CTGGGAGAAT AGTGTGGACT GCAGGGTGCC CTGGAGAGGG TGCTGACGGG
    GACTTCGCGG TCACCCACCT GACCAAGGCC CACCTGTTCC ATGACGGGCG GGTGCAGTGG
    ACTCCCCCGG CCATTTACAA GAGCTCCTGC AGCATCGACG TCACCTTCTT CCCCTTCGAC
    CAGCAGAACT GCACCATGAA ATTCGGCTCC TGGACCTACG ACAAGGCCAA GATCGACCTG
    GTGAACATGC ACAGCCGCGT GGACCAGCTG GACTTCTGGG AGAGTGGCGA GTGGGTCATC
    GTGGATGCCG TGGGCACCTA CAACACCAGG AAGTACGAGT GCTGTGCCGA GATCTACCCG
    GACATCACCT ATGCCTTCGT CATCCGGCGG CTGCCGCTCT TCTACACCAT CAACCTCATC
    ATCCCCTGCC TGCTCATCTC CTGCCTCACC GTGCTGGTCT TCTACCTGCC CTCCGAGTGT
    GGCGAGAAGA TCACGCTGTG CATCTCCGTG CTGCTGTCGC TCACCGTCTT CCTGCTGCTC
    ATCACCGAGA TCATCCCGTC CACCTCACTG GTCATCCCAC TCATCGGCGA GTACCTGCTG
    TTCACCATGA TCTTCGTCAC CCTGTCCATC GTCATCACGG TCTTCGTGCT CAACGTGCAC
    CACCGCTCGC CACGCACGCA CACCATGCCC ACCTGGGTAC GCAGGGTCTT CCTGGACATC
    GTGCCACGCC TGCTCCTCAT GAAGCGGCCG TCCGTGGTCA AGGACAATTG CCGGCGGCTC
    ATCGAGTCCA TGCATAAGAT GGCCAGTGCC CCGCGCTTCT GGCCCGAGCC AGAAGGGGAG
    CCCCCTGCCA CGAGCGGCAC CCAGAGCCTG CACCCGCCCT CACCGTCCTT CTGTGTCCCC
    CTGGATGTGC CGGCTGAGCC TGGGCCTTCC TGCAAGTCAC CCTCCGACCA GCTCCCTCCT
    CAGCAGCCCC TGGAAGCTGA GAAAGCCAGC CCCCACCCCT CGCCTGGACC CTGCCGCCCG
    CCCCACGGCA CCCAGGCACC AGGGCTGGCC AAAGCCAGGT CCCTCAGCGT CCAGCACATG
    TCCAGCCCTG GCGAAGCGGT GGAAGGCGGC GTCCGGTGCC GGTCTCGGAG CATCCAGTAC
    TGTGTTCCCC GAGACGATGC CGCCCCCGAG GCAGATGGCC AGGCTGCCGG CGCCCTGGCC
    TCTCGCAACA CCCACTCGGC TGAGCTCCCA CCCCCAGACC AGCCCTCTCC GTGCAAATGC
    ACATGCAAGA AGGAGCCCTC TTCGGTGTCC CCGAGCGCCA CGGTCAAGAC CCGCAGCACC
    AAAGCGCCGC CCCCGCACCT GCCCCTGTCG CCGGCCCTGA CCCGGGCGGT GGAGGGCGTC
    CAGTACATTG CAGACCACCT GAAGGCCGAA GACACAGACT TCTCGGTGAA GGAGGACTGG
    AAGTACGTGG CCATGGTCAT CGACCGCATC TTCCTCTGGA TGTTCATCAT CGTCTGCCTG
    CTGGGGACGG TGGGCCTCTT CCTGCCGCCC TGGCTGGCTG GCATGATCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92900
    Clone ID Related Accession (Same CDS sequence) XM_017027624.1
    Accession Version XM_017027624.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1641bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit alpha-4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGATGACCA CGAACGTATG GGTGAAGCAG GAGTGGCACG ACTACAAGCT GCGCTGGGAC 
    CCAGCTGACT ATGAGAATGT CACCTCCATC CGCATCCCCT CCGAGCTCAT CTGGCGGCCG
    GACATCGTCC TCTACAACAA TGCTGACGGG GACTTCGCGG TCACCCACCT GACCAAGGCC
    CACCTGTTCC ATGACGGGCG GGTGCAGTGG ACTCCCCCGG CCATTTACAA GAGCTCCTGC
    AGCATCGACG TCACCTTCTT CCCCTTCGAC CAGCAGAACT GCACCATGAA ATTCGGCTCC
    TGGACCTACG ACAAGGCCAA GATCGACCTG GTGAACATGC ACAGCCGCGT GGACCAGCTG
    GACTTCTGGG AGAGTGGCGA GTGGGTCATC GTGGATGCCG TGGGCACCTA CAACACCAGG
    AAGTACGAGT GCTGTGCCGA GATCTACCCG GACATCACCT ATGCCTTCGT CATCCGGCGG
    CTGCCGCTCT TCTACACCAT CAACCTCATC ATCCCCTGCC TGCTCATCTC CTGCCTCACC
    GTGCTGGTCT TCTACCTGCC CTCCGAGTGT GGCGAGAAGA TCACGCTGTG CATCTCCGTG
    CTGCTGTCGC TCACCGTCTT CCTGCTGCTC ATCACCGAGA TCATCCCGTC CACCTCACTG
    GTCATCCCAC TCATCGGCGA GTACCTGCTG TTCACCATGA TCTTCGTCAC CCTGTCCATC
    GTCATCACGG TCTTCGTGCT CAACGTGCAC CACCGCTCGC CACGCACGCA CACCATGCCC
    ACCTGGGTAC GCAGGGTCTT CCTGGACATC GTGCCACGCC TGCTCCTCAT GAAGCGGCCG
    TCCGTGGTCA AGGACAATTG CCGGCGGCTC ATCGAGTCCA TGCATAAGAT GGCCAGTGCC
    CCGCGCTTCT GGCCCGAGCC AGAAGGGGAG CCCCCTGCCA CGAGCGGCAC CCAGAGCCTG
    CACCCGCCCT CACCGTCCTT CTGTGTCCCC CTGGATGTGC CGGCTGAGCC TGGGCCTTCC
    TGCAAGTCAC CCTCCGACCA GCTCCCTCCT CAGCAGCCCC TGGAAGCTGA GAAAGCCAGC
    CCCCACCCCT CGCCTGGACC CTGCCGCCCG CCCCACGGCA CCCAGGCACC AGGGCTGGCC
    AAAGCCAGGT CCCTCAGCGT CCAGCACATG TCCAGCCCTG GCGAAGCGGT GGAAGGCGGC
    GTCCGGTGCC GGTCTCGGAG CATCCAGTAC TGTGTTCCCC GAGACGATGC CGCCCCCGAG
    GCAGATGGCC AGGCTGCCGG CGCCCTGGCC TCTCGCAACA CCCACTCGGC TGAGCTCCCA
    CCCCCAGACC AGCCCTCTCC GTGCAAATGC ACATGCAAGA AGGAGCCCTC TTCGGTGTCC
    CCGAGCGCCA CGGTCAAGAC CCGCAGCACC AAAGCGCCGC CCCCGCACCT GCCCCTGTCG
    CCGGCCCTGA CCCGGGCGGT GGAGGGCGTC CAGTACATTG CAGACCACCT GAAGGCCGAA
    GACACAGACT TCTCGGTGAA GGAGGACTGG AAGTACGTGG CCATGGTCAT CGACCGCATC
    TTCCTCTGGA TGTTCATCAT CGTCTGCCTG CTGGGGACGG TGGGCCTCTT CCTGCCGCCC
    TGGCTGGCTG GCATGATCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016883113.1
    CDS507..2147
    Translation

    Target ORF information:

    RefSeq Version XM_017027624.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017027624.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGATGACCA CGAACGTATG GGTGAAGCAG GAGTGGCACG ACTACAAGCT GCGCTGGGAC 
    CCAGCTGACT ATGAGAATGT CACCTCCATC CGCATCCCCT CCGAGCTCAT CTGGCGGCCG
    GACATCGTCC TCTACAACAA TGCTGACGGG GACTTCGCGG TCACCCACCT GACCAAGGCC
    CACCTGTTCC ATGACGGGCG GGTGCAGTGG ACTCCCCCGG CCATTTACAA GAGCTCCTGC
    AGCATCGACG TCACCTTCTT CCCCTTCGAC CAGCAGAACT GCACCATGAA ATTCGGCTCC
    TGGACCTACG ACAAGGCCAA GATCGACCTG GTGAACATGC ACAGCCGCGT GGACCAGCTG
    GACTTCTGGG AGAGTGGCGA GTGGGTCATC GTGGATGCCG TGGGCACCTA CAACACCAGG
    AAGTACGAGT GCTGTGCCGA GATCTACCCG GACATCACCT ATGCCTTCGT CATCCGGCGG
    CTGCCGCTCT TCTACACCAT CAACCTCATC ATCCCCTGCC TGCTCATCTC CTGCCTCACC
    GTGCTGGTCT TCTACCTGCC CTCCGAGTGT GGCGAGAAGA TCACGCTGTG CATCTCCGTG
    CTGCTGTCGC TCACCGTCTT CCTGCTGCTC ATCACCGAGA TCATCCCGTC CACCTCACTG
    GTCATCCCAC TCATCGGCGA GTACCTGCTG TTCACCATGA TCTTCGTCAC CCTGTCCATC
    GTCATCACGG TCTTCGTGCT CAACGTGCAC CACCGCTCGC CACGCACGCA CACCATGCCC
    ACCTGGGTAC GCAGGGTCTT CCTGGACATC GTGCCACGCC TGCTCCTCAT GAAGCGGCCG
    TCCGTGGTCA AGGACAATTG CCGGCGGCTC ATCGAGTCCA TGCATAAGAT GGCCAGTGCC
    CCGCGCTTCT GGCCCGAGCC AGAAGGGGAG CCCCCTGCCA CGAGCGGCAC CCAGAGCCTG
    CACCCGCCCT CACCGTCCTT CTGTGTCCCC CTGGATGTGC CGGCTGAGCC TGGGCCTTCC
    TGCAAGTCAC CCTCCGACCA GCTCCCTCCT CAGCAGCCCC TGGAAGCTGA GAAAGCCAGC
    CCCCACCCCT CGCCTGGACC CTGCCGCCCG CCCCACGGCA CCCAGGCACC AGGGCTGGCC
    AAAGCCAGGT CCCTCAGCGT CCAGCACATG TCCAGCCCTG GCGAAGCGGT GGAAGGCGGC
    GTCCGGTGCC GGTCTCGGAG CATCCAGTAC TGTGTTCCCC GAGACGATGC CGCCCCCGAG
    GCAGATGGCC AGGCTGCCGG CGCCCTGGCC TCTCGCAACA CCCACTCGGC TGAGCTCCCA
    CCCCCAGACC AGCCCTCTCC GTGCAAATGC ACATGCAAGA AGGAGCCCTC TTCGGTGTCC
    CCGAGCGCCA CGGTCAAGAC CCGCAGCACC AAAGCGCCGC CCCCGCACCT GCCCCTGTCG
    CCGGCCCTGA CCCGGGCGGT GGAGGGCGTC CAGTACATTG CAGACCACCT GAAGGCCGAA
    GACACAGACT TCTCGGTGAA GGAGGACTGG AAGTACGTGG CCATGGTCAT CGACCGCATC
    TTCCTCTGGA TGTTCATCAT CGTCTGCCTG CTGGGGACGG TGGGCCTCTT CCTGCCGCCC
    TGGCTGGCTG GCATGATCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.