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CHRNB2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol CHRNB2
Entrez Gene ID 1141
Full Name cholinergic receptor, nicotinic, beta 2 (neuronal)
Synonyms EFNL3, nAChRB2
General protein information
Preferred Names
neuronal acetylcholine receptor subunit beta-2
Names
neuronal acetylcholine receptor subunit beta-2
neuronal nicotinic acetylcholine receptor beta 2
acetylcholine receptor, nicotinic, beta 2 (neuronal)
cholinergic receptor, nicotinic, beta polypeptide 2 (neuronal)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1q21.3

Summary Neuronal acetylcholine receptors are homo- or heteropentameric complexes composed of homologous alpha and beta subunits. They belong to a superfamily of ligand-gated ion channels which allow the flow of sodium and potassium across the plasma membrane in response to ligands such as acetylcholine and nicotine. This gene encodes one of several beta subunits. Mutations in this gene are associated with autosomal dominant nocturnal frontal lobe epilepsy. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

118507

Disorder Html: Epilepsy, nocturnal frontal lobe, 3, 605375 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000748 NP_000739 neuronal acetylcholine receptor subunit beta-2 precursor



Homo sapiens (human) CHRNB2 NP_000739.1
Pan troglodytes (chimpanzee) CHRNB2 XP_001152392.1
Macaca mulatta (Rhesus monkey) CHRNB2 XP_001114439.1
Canis lupus familiaris (dog) CHRNB2 XP_547565.2
Bos taurus (cattle) CHRNB2 NP_001179403.1
Mus musculus (house mouse) Chrnb2 NP_033732.2
Rattus norvegicus (Norway rat) Chrnb2 NP_062170.1
Gallus gallus (chicken) CHRNB2 NP_990144.1
Danio rerio (zebrafish) chrnb2b XP_005169811.1
Danio rerio (zebrafish) LOC101882975 XP_005174531.1
Drosophila melanogaster (fruit fly) nAcRalpha-96Aa NP_524481.2
Xenopus (Silurana) tropicalis (western clawed frog) chrnb2 NP_001093684.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CHRNB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu27237
NM_000748 Homo sapiens cholinergic receptor, nicotinic, beta 2 (neuronal) (CHRNB2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu27237
Clone ID Related Accession (Same CDS sequence) NM_000748
Accession Version NM_000748.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1509bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product neuronal acetylcholine receptor subunit beta-2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62437.1, AL592078.10 and R40506.1. This sequence is a reference standard in the RefSeqGene project. On Jul 21, 2006 this sequence version replaced gi:4502832. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U62437.1, X53179.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCCGGC GCTGCGGCCC CGTGGCGCTG CTCCTTGGCT TCGGCCTCCT CCGGCTGTGC 
TCAGGGGTGT GGGGTACGGA TACAGAGGAG CGGCTGGTGG AGCATCTCCT GGATCCTTCC
CGCTACAACA AGCTTATCCG CCCAGCCACC AATGGCTCTG AGCTGGTGAC AGTACAGCTT
ATGGTGTCAC TGGCCCAGCT CATCAGTGTG CATGAGCGGG AGCAGATCAT GACCACCAAT
GTCTGGCTGA CCCAGGAGTG GGAAGATTAT CGCCTCACCT GGAAGCCTGA AGAGTTTGAC
AACATGAAGA AAGTTCGGCT CCCTTCCAAA CACATCTGGC TCCCAGATGT GGTCCTGTAC
AACAATGCTG ACGGCATGTA CGAGGTGTCC TTCTATTCCA ATGCCGTGGT CTCCTATGAT
GGCAGCATCT TCTGGCTGCC GCCTGCCATC TACAAGAGCG CATGCAAGAT TGAAGTAAAG
CACTTCCCAT TTGACCAGCA GAACTGCACC ATGAAGTTCC GTTCGTGGAC CTACGACCGC
ACAGAGATCG ACTTGGTGCT GAAGAGTGAG GTGGCCAGCC TGGACGACTT CACACCTAGT
GGTGAGTGGG ACATCGTGGC GCTGCCGGGC CGGCGCAACG AGAACCCCGA CGACTCTACG
TACGTGGACA TCACGTATGA CTTCATCATT CGCCGCAAGC CGCTCTTCTA CACCATCAAC
CTCATCATCC CCTGTGTGCT CATCACCTCG CTAGCCATCC TTGTCTTCTA CCTGCCATCC
GACTGTGGCG AGAAGATGAC GTTGTGCATC TCAGTGCTGC TGGCGCTCAC GGTCTTCCTG
CTGCTCATCT CCAAGATCGT GCCTCCCACC TCCCTCGACG TGCCGCTCGT CGGCAAGTAC
CTCATGTTCA CCATGGTGCT TGTCACCTTC TCCATCGTCA CCAGCGTGTG CGTGCTCAAC
GTGCACCACC GCTCGCCCAC CACGCACACC ATGGCGCCCT GGGTGAAGGT CGTCTTCCTG
GAGAAGCTGC CCGCGCTGCT CTTCATGCAG CAGCCACGCC ATCATTGCGC CCGTCAGCGC
CTGCGCCTGC GGCGACGCCA GCGTGAGCGC GAGGGCGCTG GAGCCCTCTT CTTCCGCGAA
GCCCCAGGGG CCGACTCCTG CACGTGCTTC GTCAACCGCG CGTCGGTGCA GGGGTTGGCC
GGGGCCTTCG GGGCTGAGCC TGCACCAGTG GCGGGCCCCG GGCGCTCAGG GGAGCCGTGT
GGCTGTGGCC TCCGGGAGGC GGTGGACGGC GTGCGCTTCA TCGCAGACCA CATGCGGAGC
GAGGACGATG ACCAGAGCGT GAGTGAGGAC TGGAAGTACG TCGCCATGGT GATCGACCGC
CTCTTCCTCT GGATCTTTGT CTTTGTCTGT GTCTTTGGCA CCATCGGCAT GTTCCTGCAG
CCTCTCTTCC AGAACTACAC CACCACCACC TTCCTCCACT CAGACCACTC AGCCCCCAGC
TCCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000739.1
CDS265..1773
Misc Feature(1)112..114(+)
Misc Feature(2)349..1701(+)
Misc Feature(3)349..966(+)
Misc Feature(4)964..1038(+)
Misc Feature(5)985..1698(+)
Misc Feature(6)1060..1116(+)
Misc Feature(7)1162..1227(+)
Misc Feature(8)1639..1698(+)
Exon (1)1..328
Gene:CHRNB2
Gene Synonym:
Exon (2)329..474
Gene:CHRNB2
Gene Synonym:
Exon (3)475..519
Gene:CHRNB2
Gene Synonym:
Exon (4)520..629
Gene:CHRNB2
Gene Synonym:
Exon (5)630..1602
Gene:CHRNB2
Gene Synonym:
Exon (6)1603..5719
Gene:CHRNB2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000748
Organism Homo sapiens (human)
Definition Homo sapiens cholinergic receptor, nicotinic, beta 2 (neuronal) (CHRNB2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000748

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCCGGC GCTGCGGCCC CGTGGCGCTG CTCCTTGGCT TCGGCCTCCT CCGGCTGTGC 
TCAGGGGTGT GGGGTACGGA TACAGAGGAG CGGCTGGTGG AGCATCTCCT GGATCCTTCC
CGCTACAACA AGCTTATCCG CCCAGCCACC AATGGCTCTG AGCTGGTGAC AGTACAGCTT
ATGGTGTCAC TGGCCCAGCT CATCAGTGTG CATGAGCGGG AGCAGATCAT GACCACCAAT
GTCTGGCTGA CCCAGGAGTG GGAAGATTAT CGCCTCACCT GGAAGCCTGA AGAGTTTGAC
AACATGAAGA AAGTTCGGCT CCCTTCCAAA CACATCTGGC TCCCAGATGT GGTCCTGTAC
AACAATGCTG ACGGCATGTA CGAGGTGTCC TTCTATTCCA ATGCCGTGGT CTCCTATGAT
GGCAGCATCT TCTGGCTGCC GCCTGCCATC TACAAGAGCG CATGCAAGAT TGAAGTAAAG
CACTTCCCAT TTGACCAGCA GAACTGCACC ATGAAGTTCC GTTCGTGGAC CTACGACCGC
ACAGAGATCG ACTTGGTGCT GAAGAGTGAG GTGGCCAGCC TGGACGACTT CACACCTAGT
GGTGAGTGGG ACATCGTGGC GCTGCCGGGC CGGCGCAACG AGAACCCCGA CGACTCTACG
TACGTGGACA TCACGTATGA CTTCATCATT CGCCGCAAGC CGCTCTTCTA CACCATCAAC
CTCATCATCC CCTGTGTGCT CATCACCTCG CTAGCCATCC TTGTCTTCTA CCTGCCATCC
GACTGTGGCG AGAAGATGAC GTTGTGCATC TCAGTGCTGC TGGCGCTCAC GGTCTTCCTG
CTGCTCATCT CCAAGATCGT GCCTCCCACC TCCCTCGACG TGCCGCTCGT CGGCAAGTAC
CTCATGTTCA CCATGGTGCT TGTCACCTTC TCCATCGTCA CCAGCGTGTG CGTGCTCAAC
GTGCACCACC GCTCGCCCAC CACGCACACC ATGGCGCCCT GGGTGAAGGT CGTCTTCCTG
GAGAAGCTGC CCGCGCTGCT CTTCATGCAG CAGCCACGCC ATCATTGCGC CCGTCAGCGC
CTGCGCCTGC GGCGACGCCA GCGTGAGCGC GAGGGCGCTG GAGCCCTCTT CTTCCGCGAA
GCCCCAGGGG CCGACTCCTG CACGTGCTTC GTCAACCGCG CGTCGGTGCA GGGGTTGGCC
GGGGCCTTCG GGGCTGAGCC TGCACCAGTG GCGGGCCCCG GGCGCTCAGG GGAGCCGTGT
GGCTGTGGCC TCCGGGAGGC GGTGGACGGC GTGCGCTTCA TCGCAGACCA CATGCGGAGC
GAGGACGATG ACCAGAGCGT GAGTGAGGAC TGGAAGTACG TCGCCATGGT GATCGACCGC
CTCTTCCTCT GGATCTTTGT CTTTGTCTGT GTCTTTGGCA CCATCGGCAT GTTCCTGCAG
CCTCTCTTCC AGAACTACAC CACCACCACC TTCCTCCACT CAGACCACTC AGCCCCCAGC
TCCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Chemistry, pharmacology, and behavioral studies identify chiral cyclopropanes as selective alpha4beta2-nicotinic acetylcholine receptor partial agonists exhibiting an antidepressant profile. Part II
J. Med. Chem. 56 (13), 5495-5504 (2013)
Zhang HK, Yu LF, Eaton JB, Whiteaker P, Onajole OK, Hanania T, Brunner D, Lukas RJ and Kozikowski AP.


book

Treatment for tobacco dependence: effect on brain nicotinic acetylcholine receptor density
Neuropsychopharmacology 38 (8), 1548-1556 (2013)
Brody AL, Mukhin AG, Stephanie Shulenberger, Mamoun MS, Kozman M, Phuong J, Neary M, Luu T and Mandelkern MA.


book

Possible association of nicotinic acetylcholine receptor gene (CHRNA4 and CHRNB2) polymorphisms with nicotine dependence in Japanese males: an exploratory study
Pharmacopsychiatry 46 (2), 77-82 (2013)
Chen HI, Shinkai T, Utsunomiya K, Yamada K, Sakata S, Fukunaka Y, Hwang R, De Luca V, Ohmori O, Kennedy JL, Chuang HY and Nakamura J.


book

NMR resolved multiple anesthetic binding sites in the TM domains of the alpha4beta2 nAChR
Biochim. Biophys. Acta 1828 (2), 398-404 (2013)
Bondarenko V, Mowrey D, Liu LT, Xu Y and Tang P.


book

Human alpha4beta2 nicotinic acetylcholine receptor as a novel target of oligomeric alpha-synuclein
PLoS ONE 8 (2), E55886 (2013)
Liu Q, Emadi S, Shen JX, Sierks MR and Wu J.


book

Human alpha4beta2 neuronal nicotinic acetylcholine receptor in HEK 293 cells: A patch-clamp study
J. Neurosci. 16 (24), 7880-7891 (1996)
Buisson B, Gopalakrishnan M, Arneric SP, Sullivan JP and Bertrand D.


book

Comparative structure of human neuronal alpha 2-alpha 7 and beta 2-beta 4 nicotinic acetylcholine receptor subunits and functional expression of the alpha 2, alpha 3, alpha 4, alpha 7, beta 2, and beta 4 subunits
J. Mol. Neurosci. 7 (3), 217-228 (1996)
Elliott KJ, Ellis SB, Berckhan KJ, Urrutia A, Chavez-Noriega LE, Johnson EC, Velicelebi G and Harpold MM.


book

Autosomal Dominant Nocturnal Frontal Lobe Epilepsy
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Kurahashi,H. and Hirose,S.


book

Chromosomal localization of seven neuronal nicotinic acetylcholine receptor subunit genes in humans
Genomics 13 (4), 962-967 (1992)
Anand R and Lindstrom J.


book

Nucleotide sequence of the human nicotinic acetylcholine receptor beta 2 subunit gene
Nucleic Acids Res. 18 (14), 4272 (1990)
Anand R and Lindstrom J.


 
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