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CHRNE cDNA ORF clone, Homo sapiens(Human)

Gene Symbol CHRNE
Entrez Gene ID 1145
Full Name cholinergic receptor nicotinic epsilon subunit
Synonyms ACHRE,CMS1D,CMS1E,CMS2A,CMS4A,CMS4B,CMS4C,FCCMS,SCCMS
General protein information
Preferred Names

cholinergic receptor nicotinic epsilon subunit

Names

acetylcholine receptor subunit epsilon
AchR epsilon subunit
acetylcholine receptor, nicotinic, epsilon (muscle)
cholinergic receptor, nicotinic epsilon
cholinergic receptor, nicotinic, epsilon (muscle)
cholinergic receptor, nicotinic, epsilon polypeptide

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

17

17p13.2

Summary Acetylcholine receptors at mature mammalian neuromuscular junctions are pentameric protein complexes composed of four subunits in the ratio of two alpha subunits to one beta, one epsilon, and one delta subunit. The acetylcholine receptor changes subunit composition shortly after birth when the epsilon subunit replaces the gamma subunit seen in embryonic receptors. Mutations in the epsilon subunit are associated with congenital myasthenic syndrome. [provided by RefSeq, Sep 2009].
Disorder MIM:

100725

Disorder Html: Myasthenic syndrome, slow-channel congenital, 601462 (3);

mRNA and Protein(s)

mRNA Protein Name
XM_017024115.1 XP_016879604.1 acetylcholine receptor subunit epsilon isoform X1
NM_000080.3 NP_000071.1 acetylcholine receptor subunit epsilon precursor



Homo sapiens (human) CHRNE NP_000071.1
Rattus norvegicus (Norway rat) Chrne NP_058890.1
Xenopus tropicalis (tropical clawed frog) chrne XP_002941867.2
Pan troglodytes (chimpanzee) CHRNE XP_523556.4
Canis lupus familiaris (dog) CHRNE XP_536608.3
Bos taurus (cattle) CHRNE NP_776697.1
Mus musculus (house mouse) Chrne NP_033733.1
Macaca mulatta (rhesus monkey) CHRNE XP_001097094.1


Related articles in PubMed

A new mouse model for the slow-channel congenital myasthenic syndrome induced by the AChR εL221F mutation.
Chevessier F, Peter C, Mersdorf U, Girard E, Krejci E, McArdle JJ, Witzemann V
Neurobiology of disease45(3)851-61(2012 Mar)

Congenital myasthenic syndrome associated with epidermolysis bullosa caused by homozygous mutations in PLEC1 and CHRNE.
Maselli RA, Arredondo J, Cagney O, Mozaffar T, Skinner S, Yousif S, Davis RR, Gregg JP, Sivak M, Konia TH, Thomas K, Wollmann RL
Clinical genetics80(5)444-51(2011 Nov)

Neurotoxicity induced by okadaic acid in the human neuroblastoma SH-SY5Y line can be differentially prevented by α7 and β2* nicotinic stimulation.
Del Barrio L, Martín-de-Saavedra MD, Romero A, Parada E, Egea J, Avila J, McIntosh JM, Wonnacott S, López MG
Toxicological sciences : an official journal of the Society of Toxicology123(1)193-205(2011 Sep)

Modulation of the Ca(2+) permeability of human endplate acetylcholine receptor-channel.
Piccari V, Deflorio C, Bigi R, Grassi F, Fucile S
Cell calcium49(4)272-8(2011 Apr)

Congenital myasthenic syndrome due to homozygous CHRNE mutations: report of patients in Arabia.
Salih MA, Oystreck DT, Al-Faky YH, Kabiraj M, Omer MI, Subahi EM, Beeson D, Abu-Amero KK, Bosley TM
Journal of neuro-ophthalmology : the official journal of the North American Neuro-Ophthalmology Society31(1)42-7(2011 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study provied that new mouse model for the slow-channel congenital myasthenic syndrome induced by the AChR epsilonL221F mutation.
Title: A new mouse model for the slow-channel congenital myasthenic syndrome induced by the AChR εL221F mutation.

mutational analysis of CHRNE revealed a homozygous 1293insG, which is a well-known low-expressor receptor mutation in patients with epidermolysis bullosa simplex and congenital myasthenic syndrome.
Title: Congenital myasthenic syndrome associated with epidermolysis bullosa caused by homozygous mutations in PLEC1 and CHRNE.

Targeting nAChR could offer a strategy for reducing neurodegeneration secondary to hyperphosphorylation of protein tau.
Title: Neurotoxicity induced by okadaic acid in the human neuroblastoma SH-SY5Y line can be differentially prevented by α7 and β2* nicotinic stimulation.

The mutations in the varepsilon subunit altered Ca(2+) permeability of AChR-channels, with varepsilon(L269F) increasing P(f) and varepsilon(I257F) decreasing it.
Title: Modulation of the Ca(2+) permeability of human endplate acetylcholine receptor-channel.

Three siblings have a clinical history and examination findings typical of homozygous CHRNE mutations; clinical presentation of congenital myasthenia subtypes is variable, and accurate genotyping is essential in choosing appropriate treatment.
Title: Congenital myasthenic syndrome due to homozygous CHRNE mutations: report of patients in Arabia.

The following CHRNE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu20760 NM_000080.3
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Homo sapiens cholinergic receptor nicotinic epsilon subunit (CHRNE), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu86547 XM_017024115.1
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Homo sapiens cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu20760
Clone ID Related Accession (Same CDS sequence) NM_000080.3
Accession Version NM_000080.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-22
Organism Homo sapiens(Human)
Product acetylcholine receptor subunit epsilon precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X66403.1 and AC233723.2. This sequence is a reference standard in the RefSeqGene project. On Apr 20, 2010 this sequence version replaced NM_000080.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X66403.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159607 [ECO:0000348] ##Evidence-Data-END##

1
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721
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961
1021
1081
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ATGGCAAGGG CTCCGCTTGG GGTCCTGCTC CTCTTGGGGC TTCTCGGCAG GGGTGTGGGG 
AAGAACGAGG AACTGCGTCT TTATCACCAT CTCTTCAACA ACTATGACCC AGGAAGCCGG
CCAGTGCGGG AGCCTGAGGA TACTGTCACC ATCAGCCTCA AGGTCACCCT GACGAATCTC
ATCTCACTGA ATGAAAAAGA GGAGACTCTC ACCACTAGCG TCTGGATTGG AATCGATTGG
CAGGATTACC GACTCAACTA CAGCAAGGAC GACTTTGGGG GTATAGAAAC CCTGCGAGTC
CCTTCAGAAC TCGTGTGGCT GCCAGAGATT GTGCTGGAAA ACAATATTGA TGGCCAGTTC
GGAGTGGCCT ACGACGCCAA CGTGCTCGTC TACGAGGGCG GCTCCGTGAC GTGGCTGCCT
CCGGCCATCT ACCGCAGCGT CTGCGCAGTG GAGGTCACCT ACTTCCCCTT CGATTGGCAG
AACTGTTCGC TTATTTTCCG CTCTCAGACG TACAATGCCG AAGAGGTGGA GTTCACTTTT
GCCGTAGACA ACGACGGCAA GACCATCAAC AAGATCGACA TCGACACAGA GGCCTATACT
GAGAACGGCG AGTGGGCCAT CGACTTCTGC CCGGGGGTGA TCCGCCGCCA CCACGGTGGC
GCCACCGACG GCCCAGGGGA GACTGACGTC ATCTACTCGC TCATCATCCG CCGGAAGCCG
CTCTTCTACG TCATTAACAT CATCGTGCCC TGTGTGCTCA TCTCGGGCCT GGTGCTGCTC
GCCTACTTCC TGCCGGCGCA GGCCGGCGGC CAGAAATGCA CGGTCTCCAT CAACGTCCTG
CTCGCCCAGA CCGTCTTCTT GTTCCTCATT GCCCAGAAAA TCCCAGAGAC TTCTCTGAGC
GTGCCGCTCC TGGGCAGGTT CCTTATTTTC GTCATGGTGG TCGCCACGCT CATTGTCATG
AATTGCGTCA TCGTGCTCAA CGTGTCCCAG CGGACGCCCA CCACCCACGC CATGTCCCCG
CGGCTGCGCC ACGTTCTCCT GGAGCTGCTG CCGCGCCTCC TGGGCTCCCC GCCGCCGCCC
GAGGCCCCCC GGGCCGCCTC GCCCCCAAGG CGGGCGTCGT CGGTGGGCTT ATTGCTCCGC
GCGGAGGAGC TGATACTGAA AAAGCCACGG AGCGAGCTCG TGTTTGAGGG GCAGAGGCAC
CGGCAGGGGA CCTGGACGGC TGCCTTCTGC CAGAGCCTGG GCGCCGCCGC CCCCGAGGTC
CGCTGCTGTG TGGATGCCGT GAACTTCGTG GCCGAGAGCA CGAGAGATCA GGAGGCCACC
GGCGAGGAAG TGTCCGACTG GGTGCGCATG GGGAATGCCC TTGACAACAT CTGCTTCTGG
GCCGCTCTGG TGCTCTTCAG CGTGGGCTCC AGCCTCATCT TCCTCGGGGC CTACTTCAAC
CGAGTGCCTG ATCTCCCCTA CGCGCCGTGT ATCCAGCCTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000071.1
CDS12..1493
Misc Feature(1)72..1436
Misc Feature(2)81..731
Misc Feature(3)729..803
Misc Feature(4)750..1433
Misc Feature(5)828..884
Misc Feature(6)930..995
Misc Feature(7)1380..1451
Exon (1)1..57
Exon (2)58..200
Exon (3)201..245
Exon (4)246..355
Exon (5)356..511
Exon (6)512..612
Exon (7)613..813
Exon (8)814..928
Exon (9)929..1043
Exon (10)1044..1230
Exon (11)1231..1337
Exon (12)1338..2460
Translation

Target ORF information:

RefSeq Version NM_000080.3
Organism Homo sapiens(Human)
Definition Homo sapiens cholinergic receptor nicotinic epsilon subunit (CHRNE), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000080.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAAGGG CTCCGCTTGG GGTCCTGCTC CTCTTGGGGC TTCTCGGCAG GGGTGTGGGG 
AAGAACGAGG AACTGCGTCT TTATCACCAT CTCTTCAACA ACTATGACCC AGGAAGCCGG
CCAGTGCGGG AGCCTGAGGA TACTGTCACC ATCAGCCTCA AGGTCACCCT GACGAATCTC
ATCTCACTGA ATGAAAAAGA GGAGACTCTC ACCACTAGCG TCTGGATTGG AATCGATTGG
CAGGATTACC GACTCAACTA CAGCAAGGAC GACTTTGGGG GTATAGAAAC CCTGCGAGTC
CCTTCAGAAC TCGTGTGGCT GCCAGAGATT GTGCTGGAAA ACAATATTGA TGGCCAGTTC
GGAGTGGCCT ACGACGCCAA CGTGCTCGTC TACGAGGGCG GCTCCGTGAC GTGGCTGCCT
CCGGCCATCT ACCGCAGCGT CTGCGCAGTG GAGGTCACCT ACTTCCCCTT CGATTGGCAG
AACTGTTCGC TTATTTTCCG CTCTCAGACG TACAATGCCG AAGAGGTGGA GTTCACTTTT
GCCGTAGACA ACGACGGCAA GACCATCAAC AAGATCGACA TCGACACAGA GGCCTATACT
GAGAACGGCG AGTGGGCCAT CGACTTCTGC CCGGGGGTGA TCCGCCGCCA CCACGGTGGC
GCCACCGACG GCCCAGGGGA GACTGACGTC ATCTACTCGC TCATCATCCG CCGGAAGCCG
CTCTTCTACG TCATTAACAT CATCGTGCCC TGTGTGCTCA TCTCGGGCCT GGTGCTGCTC
GCCTACTTCC TGCCGGCGCA GGCCGGCGGC CAGAAATGCA CGGTCTCCAT CAACGTCCTG
CTCGCCCAGA CCGTCTTCTT GTTCCTCATT GCCCAGAAAA TCCCAGAGAC TTCTCTGAGC
GTGCCGCTCC TGGGCAGGTT CCTTATTTTC GTCATGGTGG TCGCCACGCT CATTGTCATG
AATTGCGTCA TCGTGCTCAA CGTGTCCCAG CGGACGCCCA CCACCCACGC CATGTCCCCG
CGGCTGCGCC ACGTTCTCCT GGAGCTGCTG CCGCGCCTCC TGGGCTCCCC GCCGCCGCCC
GAGGCCCCCC GGGCCGCCTC GCCCCCAAGG CGGGCGTCGT CGGTGGGCTT ATTGCTCCGC
GCGGAGGAGC TGATACTGAA AAAGCCACGG AGCGAGCTCG TGTTTGAGGG GCAGAGGCAC
CGGCAGGGGA CCTGGACGGC TGCCTTCTGC CAGAGCCTGG GCGCCGCCGC CCCCGAGGTC
CGCTGCTGTG TGGATGCCGT GAACTTCGTG GCCGAGAGCA CGAGAGATCA GGAGGCCACC
GGCGAGGAAG TGTCCGACTG GGTGCGCATG GGGAATGCCC TTGACAACAT CTGCTTCTGG
GCCGCTCTGG TGCTCTTCAG CGTGGGCTCC AGCCTCATCT TCCTCGGGGC CTACTTCAAC
CGAGTGCCTG ATCTCCCCTA CGCGCCGTGT ATCCAGCCTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu86547
Clone ID Related Accession (Same CDS sequence) XM_017024115.1
Accession Version XM_017024115.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1446bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product acetylcholine receptor subunit epsilon isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCAAAGAG GCAGGGGTGT GGGGAAGAAC GAGGAACTGC GTCTTTATCA CCATCTCTTC 
AACAACTATG ACCCAGGAAG CCGGCCAGTG CGGGAGCCTG AGGATACTGT CACCATCAGC
CTCAAGGTCA CCCTGACGAA TCTCATCTCA CTGAATGAAA AAGAGGAGAC TCTCACCACT
AGCGTCTGGA TTGGAATCGA TTGGCAGGAT TACCGACTCA ACTACAGCAA GGACGACTTT
GGGGGTATAG AAACCCTGCG AGTCCCTTCA GAACTCGTGT GGCTGCCAGA GATTGTGCTG
GAAAACAATA TTGATGGCCA GTTCGGAGTG GCCTACGACG CCAACGTGCT CGTCTACGAG
GGCGGCTCCG TGACGTGGCT GCCTCCGGCC ATCTACCGCA GCGTCTGCGC AGTGGAGGTC
ACCTACTTCC CCTTCGATTG GCAGAACTGT TCGCTTATTT TCCGCTCTCA GACGTACAAT
GCCGAAGAGG TGGAGTTCAC TTTTGCCGTA GACAACGACG GCAAGACCAT CAACAAGATC
GACATCGACA CAGAGGCCTA TACTGAGAAC GGCGAGTGGG CCATCGACTT CTGCCCGGGG
GTGATCCGCC GCCACCACGG TGGCGCCACC GACGGCCCAG GGGAGACTGA CGTCATCTAC
TCGCTCATCA TCCGCCGGAA GCCGCTCTTC TACGTCATTA ACATCATCGT GCCCTGTGTG
CTCATCTCGG GCCTGGTGCT GCTCGCCTAC TTCCTGCCGG CGCAGGCCGG CGGCCAGAAA
TGCACGGTCT CCATCAACGT CCTGCTCGCC CAGACCGTCT TCTTGTTCCT CATTGCCCAG
AAAATCCCAG AGACTTCTCT GAGCGTGCCG CTCCTGGGCA GGTTCCTTAT TTTCGTCATG
GTGGTCGCCA CGCTCATTGT CATGAATTGC GTCATCGTGC TCAACGTGTC CCAGCGGACG
CCCACCACCC ACGCCATGTC CCCGCGGCTG CGCCACGTTC TCCTGGAGCT GCTGCCGCGC
CTCCTGGGCT CCCCGCCGCC GCCCGAGGCC CCCCGGGCCG CCTCGCCCCC AAGGCGGGCG
TCGTCGGTGG GCTTATTGCT CCGCGCGGAG GAGCTGATAC TGAAAAAGCC ACGGAGCGAG
CTCGTGTTTG AGGGGCAGAG GCACCGGCAG GGGACCTGGA CGGCTGCCTT CTGCCAGAGC
CTGGGCGCCG CCGCCCCCGA GGTCCGCTGC TGTGTGGATG CCGTGAACTT CGTGGCCGAG
AGCACGAGAG ATCAGGAGGC CACCGGCGAG GAAGTGTCCG ACTGGGTGCG CATGGGGAAT
GCCCTTGACA ACATCTGCTT CTGGGCCGCT CTGGTGCTCT TCAGCGTGGG CTCCAGCCTC
ATCTTCCTCG GGGCCTACTT CAACCGAGTG CCTGATCTCC CCTACGCGCC GTGTATCCAG
CCTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016879604.1
CDS67..1512
Translation

Target ORF information:

RefSeq Version XM_017024115.1
Organism Homo sapiens(Human)
Definition Homo sapiens cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017024115.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCAAAGAG GCAGGGGTGT GGGGAAGAAC GAGGAACTGC GTCTTTATCA CCATCTCTTC 
AACAACTATG ACCCAGGAAG CCGGCCAGTG CGGGAGCCTG AGGATACTGT CACCATCAGC
CTCAAGGTCA CCCTGACGAA TCTCATCTCA CTGAATGAAA AAGAGGAGAC TCTCACCACT
AGCGTCTGGA TTGGAATCGA TTGGCAGGAT TACCGACTCA ACTACAGCAA GGACGACTTT
GGGGGTATAG AAACCCTGCG AGTCCCTTCA GAACTCGTGT GGCTGCCAGA GATTGTGCTG
GAAAACAATA TTGATGGCCA GTTCGGAGTG GCCTACGACG CCAACGTGCT CGTCTACGAG
GGCGGCTCCG TGACGTGGCT GCCTCCGGCC ATCTACCGCA GCGTCTGCGC AGTGGAGGTC
ACCTACTTCC CCTTCGATTG GCAGAACTGT TCGCTTATTT TCCGCTCTCA GACGTACAAT
GCCGAAGAGG TGGAGTTCAC TTTTGCCGTA GACAACGACG GCAAGACCAT CAACAAGATC
GACATCGACA CAGAGGCCTA TACTGAGAAC GGCGAGTGGG CCATCGACTT CTGCCCGGGG
GTGATCCGCC GCCACCACGG TGGCGCCACC GACGGCCCAG GGGAGACTGA CGTCATCTAC
TCGCTCATCA TCCGCCGGAA GCCGCTCTTC TACGTCATTA ACATCATCGT GCCCTGTGTG
CTCATCTCGG GCCTGGTGCT GCTCGCCTAC TTCCTGCCGG CGCAGGCCGG CGGCCAGAAA
TGCACGGTCT CCATCAACGT CCTGCTCGCC CAGACCGTCT TCTTGTTCCT CATTGCCCAG
AAAATCCCAG AGACTTCTCT GAGCGTGCCG CTCCTGGGCA GGTTCCTTAT TTTCGTCATG
GTGGTCGCCA CGCTCATTGT CATGAATTGC GTCATCGTGC TCAACGTGTC CCAGCGGACG
CCCACCACCC ACGCCATGTC CCCGCGGCTG CGCCACGTTC TCCTGGAGCT GCTGCCGCGC
CTCCTGGGCT CCCCGCCGCC GCCCGAGGCC CCCCGGGCCG CCTCGCCCCC AAGGCGGGCG
TCGTCGGTGG GCTTATTGCT CCGCGCGGAG GAGCTGATAC TGAAAAAGCC ACGGAGCGAG
CTCGTGTTTG AGGGGCAGAG GCACCGGCAG GGGACCTGGA CGGCTGCCTT CTGCCAGAGC
CTGGGCGCCG CCGCCCCCGA GGTCCGCTGC TGTGTGGATG CCGTGAACTT CGTGGCCGAG
AGCACGAGAG ATCAGGAGGC CACCGGCGAG GAAGTGTCCG ACTGGGTGCG CATGGGGAAT
GCCCTTGACA ACATCTGCTT CTGGGCCGCT CTGGTGCTCT TCAGCGTGGG CTCCAGCCTC
ATCTTCCTCG GGGCCTACTT CAACCGAGTG CCTGATCTCC CCTACGCGCC GTGTATCCAG
CCTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

A new mouse model for the slow-channel congenital myasthenic syndrome induced by the AChR εL221F mutation.
Neurobiology of disease45(3)851-61(2012 Mar)
Chevessier F,Peter C,Mersdorf U,Girard E,Krejci E,McArdle JJ,Witzemann V


book

Congenital myasthenic syndrome associated with epidermolysis bullosa caused by homozygous mutations in PLEC1 and CHRNE.
Clinical genetics80(5)444-51(2011 Nov)
Maselli RA,Arredondo J,Cagney O,Mozaffar T,Skinner S,Yousif S,Davis RR,Gregg JP,Sivak M,Konia TH,Thomas K,Wollmann RL


book

Neurotoxicity induced by okadaic acid in the human neuroblastoma SH-SY5Y line can be differentially prevented by α7 and β2* nicotinic stimulation.
Toxicological sciences : an official journal of the Society of Toxicology123(1)193-205(2011 Sep)
Del Barrio L,Martín-de-Saavedra MD,Romero A,Parada E,Egea J,Avila J,McIntosh JM,Wonnacott S,López MG


book

Modulation of the Ca(2+) permeability of human endplate acetylcholine receptor-channel.
Cell calcium49(4)272-8(2011 Apr)
Piccari V,Deflorio C,Bigi R,Grassi F,Fucile S


book

Congenital myasthenic syndrome due to homozygous CHRNE mutations: report of patients in Arabia.
Journal of neuro-ophthalmology : the official journal of the North American Neuro-Ophthalmology Society31(1)42-7(2011 Mar)
Salih MA,Oystreck DT,Al-Faky YH,Kabiraj M,Omer MI,Subahi EM,Beeson D,Abu-Amero KK,Bosley TM


 
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