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MIEF2 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol MIEF2
Entrez Gene ID 125170
Full Name mitochondrial elongation factor 2
Synonyms MID49,SMCR7
General protein information
Preferred Names

mitochondrial elongation factor 2

Names

mitochondrial dynamics protein MID49
Smith-Magenis syndrome chromosomal region candidate gene 7 protein
Smith-Magenis syndrome chromosome region, candidate 7
mitochondrial dynamic protein MID49
mitochondrial dynamic protein of 49 kDa
mitochondrial dynamics protein of 49 kDa
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

17

17p11.2
Summary This gene encodes an outer mitochondrial membrane protein that functions in the regulation of mitochondrial morphology. It can directly recruit the fission mediator dynamin-related protein 1 (Drp1) to the mitochondrial surface. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011].
Disorder MIM:

615498


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_139162.4 NP_631901.2 mitochondrial dynamics protein MID49 isoform 1
XM_017024190.1 XP_016879679.1 mitochondrial dynamics protein MID49 isoform X1
NM_001144900.2 NP_001138372.1 mitochondrial dynamics protein MID49 isoform 3
NM_148886.1 NP_683684.2 mitochondrial dynamics protein MID49 isoform 2
NM_139162.3 NP_631901.2 mitochondrial dynamics protein MID49 isoform 1
NM_001144900.1 NP_001138372.1 mitochondrial dynamics protein MID49 isoform 3

Pathways from BioSystems


Homologies


Homo sapiens (human) MIEF2 NP_683684.2
Mus musculus (house mouse) Mief2 NP_001009927.1
Rattus norvegicus (Norway rat) Mief2 XP_006246605.1
SMCR7 XP_001095096.1
Canis lupus familiaris (dog) SMCR7 XP_005620202.1
Xenopus tropicalis (tropical clawed frog) mief2 XP_002935891.1
Pan troglodytes (chimpanzee) SMCR7 XP_003953167.1
Gallus gallus (chicken) SMCR7 XP_414820.4

Gene Ontology


Bibliography

Related articles in PubMed

Foxo3a inhibits mitochondrial fission and protects against doxorubicin-induced cardiotoxicity by suppressing MIEF2.
Zhou L, Li R, Liu C, Sun T, Htet Aung LH, Chen C, Gao J, Zhao Y, Wang K
Free radical biology & medicine104360-370(2017 Mar)

The role of Drp1 adaptor proteins MiD49 and MiD51?in mitochondrial fission: implications for human disease.
Atkins K, Dasgupta A, Chen KH, Mewburn J, Archer SL
Clinical science (London, England : 1979)130(21)1861-74(2016 Nov)

Drp1-dependent mitochondrial fission via MiD49/51 is essential for apoptotic cristae remodeling.
Otera H, Miyata N, Kuge O, Mihara K
The Journal of cell biology212(5)531-44(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knockdown of MIEF2 reduces DOX-induced mitochondrial fission and apoptosis in cardiomyocytes and in vivo. Also, knockdown of MIEF2 protects heart from DOX-induced cardiotoxicity. Our study identifies a novel pathway composed of Foxo3a and MIEF2 that mediates DOX cardiotoxicity.
Title: Foxo3a inhibits mitochondrial fission and protects against doxorubicin-induced cardiotoxicity by suppressing MIEF2.

MiD49 and MiD51 recruit inactive forms of Drp1 in mitochondrial fission. [review]
Title: The role of Drp1 adaptor proteins MiD49 and MiD51?in mitochondrial fission: implications for human disease.

The results indicate that Drp1-dependent mitochondrial fission through MiD49/MiD51 regulates cristae remodeling during intrinsic apoptosis.
Title: Drp1-dependent mitochondrial fission via MiD49/51 is essential for apoptotic cristae remodeling.

These findings and data showing MARCH5-dependent degradation of MiD49 upon stress support the possibility that MARCH5 regulation of MiD49 is a novel mechanism controlling mitochondrial fission and, consequently, the cellular response to stress.
Title: Mitochondrial E3 ubiquitin ligase MARCH5 controls mitochondrial fission and cell sensitivity to stress-induced apoptosis through regulation of MiD49 protein.

MiD49 and MiD51 can act independently of Mff and Fis1 in Drp1 recruitment and suggest that they provide specificity to the division of mitochondria.
Title: Adaptor proteins MiD49 and MiD51 can act independently of Mff and Fis1 in Drp1 recruitment and are specific for mitochondrial fission.

The following MIEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MIEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu12025 NM_139162.4
Latest version!		 Homo sapiens mitochondrial elongation factor 2 (MIEF2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $159.50-$223.30
$319.00
OHu00199 NM_001144900.2
Latest version!		 Homo sapiens mitochondrial elongation factor 2 (MIEF2), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu11703 NM_148886.1
Latest version!		 Homo sapiens mitochondrial elongation factor 2 (MIEF2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu86598 XM_017024190.1
Latest version!		 Homo sapiens mitochondrial elongation factor 2 (MIEF2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
View Old Accession Versions >>
OHu12025 NM_139162.3 Homo sapiens mitochondrial elongation factor 2 (MIEF2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $159.50-$223.30
$319.00
OHu00199 NM_001144900.1 Homo sapiens mitochondrial elongation factor 2 (MIEF2), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu12025
Clone ID Related Accession (Same CDS sequence) NM_139162.4 , NM_139162.3
Accession Version NM_139162.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1365bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-05-01
Organism Homo sapiens(human)
Product mitochondrial dynamics protein MID49 isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK056165.1, AC127537.8 and BC014973.1. On Nov 24, 2018 this sequence version replaced NM_139162.3. Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.216912.1, SRR1803616.190074.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology MANE Ensembl match :: ENST00000323019.9/ ENSP00000323591.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCAGAGT TCTCCCAGAA ACGGGGGAAG CGGCGTAGCG ACGAAGGGCT GGGCAGCATG 
GTGGACTTCC TCCTGGCCAA TGCCCGCCTG GTGCTGGGCG TGGGCGGGGC TGCTGTGCTG 
GGCATTGCCA CCCTGGCCGT GAAGCGGTTC ATTGACAGGG CCACTAGCCC GCGGGATGAG 
GATGACACCA AGGCAGACAG CTGGAAGGAA CTGAGCCTGC TCAAGGCCAC ACCACACCTG 
CAGCCCCGGC CTCCACCTGC TGCCCTTAGC CAGCCAGTGT TGCCCTTGGC CCCCTCGTCG 
TCTGCCCCAG AAGGGCCTGC AGAAACTGAT CCTGAGGTGA CACCACAGCT CAGCTCCCCA 
GCACCGCTGT GTCTGACACT GCAGGAGAGG CTGCTGGCCT TCGAGCGGGA CCGTGTGACC 
ATCCCAGCAG CCCAGGTGGC TTTGGCCAAA CAGCTGGCTG GCGACATCGC CCTGGAGCTG 
CAGGCCTACT TTCGGAGCAA GTTCCCGGAA CTGCCCTTTG GGGCATTCGT GCCTGGGGGG 
CCGCTCTACG ACGGGCTGCA GGCGGGGGCT GCGGACCATG TGCGTCTCCT GGTGCCACTG 
GTGCTGGAGC CGGGCCTGTG GAGCCTGGTG CCGGGCGTGG ACACTGTGGC GAGGGACCCT 
CGCTGCTGGG CCGTGCGCAG GACGCAGCTT GAGTTCTGCC CCCGTGGGAG CAGCCCCTGG 
GACCGCTTCC TGGTCGGGGG CTACCTCTCC TCCCGCGTCC TGCTGGAGCT ACTCCGCAAG 
GCGCTGGCTG CTTCTGTCAA CTGGCCGGCC ATTGGCAGCC TTCTCGGGTG CCTGATCCGG 
CCCAGCATGG CCTCGGAGGA GCTGCTGCTC GAGGTGCAGC ACGAACGCCT GGAGCTCACT 
GTGGCTGTGC TTGTGGCAGT CCCTGGGGTC GATGCTGACG ACCGCCTCCT CTTGGCCTGG 
CCCCTGGAGG GGCTGGCGGG GAACCTCTGG CTGCAGGACC TGTATCCAGT GGAGGCTGCT 
AGGCTGCGAG CCCTGGACGA CCATGACGCT GGGACTCGCC GGCGGCTGCT GCTGCTGCTG 
TGTGCTGTCT GCCGTGGTTG CTCGGCTCTG GGGCAGCTAG GCCGGGGTCA CCTGACCCAG 
GTGGTCCTGC GTCTGGGGGA GGACAACGTG GATTGGACGG AGGAGGCCTT GGGTGAGCGC 
TTCCTGCAAG CCCTGGAGCT GCTCATCGGC AGCCTGGAGC AGGCCAGCCT GCCCTGCCAC 
TTCAACCCCA GCGTGAACCT CTTCAGCAGC TTGCGTGAGG AGGAGATTGA CGACATTGGC 
TATGCGCTAT ACAGTGGCCT ACAGGAGCCC GAGGGGCTGC TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_631901.2
CDS84..1448
Translation

Target ORF information:

RefSeq Version NM_139162.4
Organism Homo sapiens(human)
Definition Homo sapiens mitochondrial elongation factor 2 (MIEF2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_139162.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCAGAGT TCTCCCAGAA ACGGGGGAAG CGGCGTAGCG ACGAAGGGCT GGGCAGCATG 
GTGGACTTCC TCCTGGCCAA TGCCCGCCTG GTGCTGGGCG TGGGCGGGGC TGCTGTGCTG 
GGCATTGCCA CCCTGGCCGT GAAGCGGTTC ATTGACAGGG CCACTAGCCC GCGGGATGAG 
GATGACACCA AGGCAGACAG CTGGAAGGAA CTGAGCCTGC TCAAGGCCAC ACCACACCTG 
CAGCCCCGGC CTCCACCTGC TGCCCTTAGC CAGCCAGTGT TGCCCTTGGC CCCCTCGTCG 
TCTGCCCCAG AAGGGCCTGC AGAAACTGAT CCTGAGGTGA CACCACAGCT CAGCTCCCCA 
GCACCGCTGT GTCTGACACT GCAGGAGAGG CTGCTGGCCT TCGAGCGGGA CCGTGTGACC 
ATCCCAGCAG CCCAGGTGGC TTTGGCCAAA CAGCTGGCTG GCGACATCGC CCTGGAGCTG 
CAGGCCTACT TTCGGAGCAA GTTCCCGGAA CTGCCCTTTG GGGCATTCGT GCCTGGGGGG 
CCGCTCTACG ACGGGCTGCA GGCGGGGGCT GCGGACCATG TGCGTCTCCT GGTGCCACTG 
GTGCTGGAGC CGGGCCTGTG GAGCCTGGTG CCGGGCGTGG ACACTGTGGC GAGGGACCCT 
CGCTGCTGGG CCGTGCGCAG GACGCAGCTT GAGTTCTGCC CCCGTGGGAG CAGCCCCTGG 
GACCGCTTCC TGGTCGGGGG CTACCTCTCC TCCCGCGTCC TGCTGGAGCT ACTCCGCAAG 
GCGCTGGCTG CTTCTGTCAA CTGGCCGGCC ATTGGCAGCC TTCTCGGGTG CCTGATCCGG 
CCCAGCATGG CCTCGGAGGA GCTGCTGCTC GAGGTGCAGC ACGAACGCCT GGAGCTCACT 
GTGGCTGTGC TTGTGGCAGT CCCTGGGGTC GATGCTGACG ACCGCCTCCT CTTGGCCTGG 
CCCCTGGAGG GGCTGGCGGG GAACCTCTGG CTGCAGGACC TGTATCCAGT GGAGGCTGCT 
AGGCTGCGAG CCCTGGACGA CCATGACGCT GGGACTCGCC GGCGGCTGCT GCTGCTGCTG 
TGTGCTGTCT GCCGTGGTTG CTCGGCTCTG GGGCAGCTAG GCCGGGGTCA CCTGACCCAG 
GTGGTCCTGC GTCTGGGGGA GGACAACGTG GATTGGACGG AGGAGGCCTT GGGTGAGCGC 
TTCCTGCAAG CCCTGGAGCT GCTCATCGGC AGCCTGGAGC AGGCCAGCCT GCCCTGCCAC 
TTCAACCCCA GCGTGAACCT CTTCAGCAGC TTGCGTGAGG AGGAGATTGA CGACATTGGC 
TATGCGCTAT ACAGTGGCCT ACAGGAGCCC GAGGGGCTGC TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu12025
Clone ID Related Accession (Same CDS sequence) NM_139162.4 , NM_139162.3
Accession Version NM_139162.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1365bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-12-17
Organism Homo sapiens(human)
Product mitochondrial dynamics protein MID49 isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB183618.1, AK056165.1, AC127537.8 and BC014973.1. On Jan 30, 2009 this sequence version replaced NM_139162.2. Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DB183618.1, SRR1660803.51880.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCAGAGT TCTCCCAGAA ACGGGGGAAG CGGCGTAGCG ACGAAGGGCT GGGCAGCATG 
GTGGACTTCC TCCTGGCCAA TGCCCGCCTG GTGCTGGGCG TGGGCGGGGC TGCTGTGCTG 
GGCATTGCCA CCCTGGCCGT GAAGCGGTTC ATTGACAGGG CCACTAGCCC GCGGGATGAG 
GATGACACCA AGGCAGACAG CTGGAAGGAA CTGAGCCTGC TCAAGGCCAC ACCACACCTG 
CAGCCCCGGC CTCCACCTGC TGCCCTTAGC CAGCCAGTGT TGCCCTTGGC CCCCTCGTCG 
TCTGCCCCAG AAGGGCCTGC AGAAACTGAT CCTGAGGTGA CACCACAGCT CAGCTCCCCA 
GCACCGCTGT GTCTGACACT GCAGGAGAGG CTGCTGGCCT TCGAGCGGGA CCGTGTGACC 
ATCCCAGCAG CCCAGGTGGC TTTGGCCAAA CAGCTGGCTG GCGACATCGC CCTGGAGCTG 
CAGGCCTACT TTCGGAGCAA GTTCCCGGAA CTGCCCTTTG GGGCATTCGT GCCTGGGGGG 
CCGCTCTACG ACGGGCTGCA GGCGGGGGCT GCGGACCATG TGCGTCTCCT GGTGCCACTG 
GTGCTGGAGC CGGGCCTGTG GAGCCTGGTG CCGGGCGTGG ACACTGTGGC GAGGGACCCT 
CGCTGCTGGG CCGTGCGCAG GACGCAGCTT GAGTTCTGCC CCCGTGGGAG CAGCCCCTGG 
GACCGCTTCC TGGTCGGGGG CTACCTCTCC TCCCGCGTCC TGCTGGAGCT ACTCCGCAAG 
GCGCTGGCTG CTTCTGTCAA CTGGCCGGCC ATTGGCAGCC TTCTCGGGTG CCTGATCCGG 
CCCAGCATGG CCTCGGAGGA GCTGCTGCTC GAGGTGCAGC ACGAACGCCT GGAGCTCACT 
GTGGCTGTGC TTGTGGCAGT CCCTGGGGTC GATGCTGACG ACCGCCTCCT CTTGGCCTGG 
CCCCTGGAGG GGCTGGCGGG GAACCTCTGG CTGCAGGACC TGTATCCAGT GGAGGCTGCT 
AGGCTGCGAG CCCTGGACGA CCATGACGCT GGGACTCGCC GGCGGCTGCT GCTGCTGCTG 
TGTGCTGTCT GCCGTGGTTG CTCGGCTCTG GGGCAGCTAG GCCGGGGTCA CCTGACCCAG 
GTGGTCCTGC GTCTGGGGGA GGACAACGTG GATTGGACGG AGGAGGCCTT GGGTGAGCGC 
TTCCTGCAAG CCCTGGAGCT GCTCATCGGC AGCCTGGAGC AGGCCAGCCT GCCCTGCCAC 
TTCAACCCCA GCGTGAACCT CTTCAGCAGC TTGCGTGAGG AGGAGATTGA CGACATTGGC 
TATGCGCTAT ACAGTGGCCT ACAGGAGCCC GAGGGGCTGC TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_631901.2
CDS212..1576
Translation

Target ORF information:

RefSeq Version NM_139162.3
Organism Homo sapiens(human)
Definition Homo sapiens mitochondrial elongation factor 2 (MIEF2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_139162.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCAGAGT TCTCCCAGAA ACGGGGGAAG CGGCGTAGCG ACGAAGGGCT GGGCAGCATG 
GTGGACTTCC TCCTGGCCAA TGCCCGCCTG GTGCTGGGCG TGGGCGGGGC TGCTGTGCTG 
GGCATTGCCA CCCTGGCCGT GAAGCGGTTC ATTGACAGGG CCACTAGCCC GCGGGATGAG 
GATGACACCA AGGCAGACAG CTGGAAGGAA CTGAGCCTGC TCAAGGCCAC ACCACACCTG 
CAGCCCCGGC CTCCACCTGC TGCCCTTAGC CAGCCAGTGT TGCCCTTGGC CCCCTCGTCG 
TCTGCCCCAG AAGGGCCTGC AGAAACTGAT CCTGAGGTGA CACCACAGCT CAGCTCCCCA 
GCACCGCTGT GTCTGACACT GCAGGAGAGG CTGCTGGCCT TCGAGCGGGA CCGTGTGACC 
ATCCCAGCAG CCCAGGTGGC TTTGGCCAAA CAGCTGGCTG GCGACATCGC CCTGGAGCTG 
CAGGCCTACT TTCGGAGCAA GTTCCCGGAA CTGCCCTTTG GGGCATTCGT GCCTGGGGGG 
CCGCTCTACG ACGGGCTGCA GGCGGGGGCT GCGGACCATG TGCGTCTCCT GGTGCCACTG 
GTGCTGGAGC CGGGCCTGTG GAGCCTGGTG CCGGGCGTGG ACACTGTGGC GAGGGACCCT 
CGCTGCTGGG CCGTGCGCAG GACGCAGCTT GAGTTCTGCC CCCGTGGGAG CAGCCCCTGG 
GACCGCTTCC TGGTCGGGGG CTACCTCTCC TCCCGCGTCC TGCTGGAGCT ACTCCGCAAG 
GCGCTGGCTG CTTCTGTCAA CTGGCCGGCC ATTGGCAGCC TTCTCGGGTG CCTGATCCGG 
CCCAGCATGG CCTCGGAGGA GCTGCTGCTC GAGGTGCAGC ACGAACGCCT GGAGCTCACT 
GTGGCTGTGC TTGTGGCAGT CCCTGGGGTC GATGCTGACG ACCGCCTCCT CTTGGCCTGG 
CCCCTGGAGG GGCTGGCGGG GAACCTCTGG CTGCAGGACC TGTATCCAGT GGAGGCTGCT 
AGGCTGCGAG CCCTGGACGA CCATGACGCT GGGACTCGCC GGCGGCTGCT GCTGCTGCTG 
TGTGCTGTCT GCCGTGGTTG CTCGGCTCTG GGGCAGCTAG GCCGGGGTCA CCTGACCCAG 
GTGGTCCTGC GTCTGGGGGA GGACAACGTG GATTGGACGG AGGAGGCCTT GGGTGAGCGC 
TTCCTGCAAG CCCTGGAGCT GCTCATCGGC AGCCTGGAGC AGGCCAGCCT GCCCTGCCAC 
TTCAACCCCA GCGTGAACCT CTTCAGCAGC TTGCGTGAGG AGGAGATTGA CGACATTGGC 
TATGCGCTAT ACAGTGGCCT ACAGGAGCCC GAGGGGCTGC TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu00199
Clone ID Related Accession (Same CDS sequence) NM_001144900.2 , NM_001144900.1
Accession Version NM_001144900.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 618bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-05-31
Organism Homo sapiens(human)
Product mitochondrial dynamics protein MID49 isoform 3
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB183618.1, AK128310.1, AC127537.8 and BC014973.1. On Jun 1, 2019 this sequence version replaced NM_001144900.1. Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region that causes a translational frameshift, compared to variant 1. The resulting isoform (3) contains a distinct C-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK128310.1, DA153706.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149004, SAMEA2152719 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
ATGGCAGAGT TCTCCCAGAA ACGGGGGAAG CGGCGTAGCG ACGAAGGGCT GGGCAGCATG 
GTGGACTTCC TCCTGGCCAA TGCCCGCCTG GTGCTGGGCG TGGGCGGGGC TGCTGTGCTG 
GGCATTGCCA CCCTGGCCGT GAAGCGGTTC ATTGACAGGG CCACTAGCCC GCGGGATGAG 
GATGACACCA AGGCAGACAG CTGGAAGGAA CTGAGCCTGC TCAAGGCCAC ACCACACCTG 
CAGCCCCGGC CTCCACCTGC TGCCCTTAGC CAGCCAGTGT TGCCCTTGGC CCCCTCGTCG 
TCTGCCCCAG GAGAGGCTGC TGGCCTTCGA GCGGGACCGT GTGACCATCC CAGCAGCCCA 
GGTGGCTTTG GCCAAACAGC TGGCTGGCGA CATCGCCCTG GAGCTGCAGG CCTACTTTCG 
GAGCAAGTTC CCGGAACTGC CCTTTGGGGC ATTCGTGCCT GGGGGGCCGC TCTACGACGG 
GCTGCAGGCG GGGGCTGCGG ACCATGTGCG TCTCCTGGTG CCACTGGTGC TGGAGCCGGG 
CCTGTGGAGC CTGGTGCCGG GCGTGGACAC TGTGGCGAGG GACCCTCGCT GCTGGGCCGT 
GCGCAGGACG CAGCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001138372.1
CDS84..701
Translation

Target ORF information:

RefSeq Version NM_001144900.2
Organism Homo sapiens(human)
Definition Homo sapiens mitochondrial elongation factor 2 (MIEF2), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001144900.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCAGAGT TCTCCCAGAA ACGGGGGAAG CGGCGTAGCG ACGAAGGGCT GGGCAGCATG 
GTGGACTTCC TCCTGGCCAA TGCCCGCCTG GTGCTGGGCG TGGGCGGGGC TGCTGTGCTG 
GGCATTGCCA CCCTGGCCGT GAAGCGGTTC ATTGACAGGG CCACTAGCCC GCGGGATGAG 
GATGACACCA AGGCAGACAG CTGGAAGGAA CTGAGCCTGC TCAAGGCCAC ACCACACCTG 
CAGCCCCGGC CTCCACCTGC TGCCCTTAGC CAGCCAGTGT TGCCCTTGGC CCCCTCGTCG 
TCTGCCCCAG GAGAGGCTGC TGGCCTTCGA GCGGGACCGT GTGACCATCC CAGCAGCCCA 
GGTGGCTTTG GCCAAACAGC TGGCTGGCGA CATCGCCCTG GAGCTGCAGG CCTACTTTCG 
GAGCAAGTTC CCGGAACTGC CCTTTGGGGC ATTCGTGCCT GGGGGGCCGC TCTACGACGG 
GCTGCAGGCG GGGGCTGCGG ACCATGTGCG TCTCCTGGTG CCACTGGTGC TGGAGCCGGG 
CCTGTGGAGC CTGGTGCCGG GCGTGGACAC TGTGGCGAGG GACCCTCGCT GCTGGGCCGT 
GCGCAGGACG CAGCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu00199
Clone ID Related Accession (Same CDS sequence) NM_001144900.2 , NM_001144900.1
Accession Version NM_001144900.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 618bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-20
Organism Homo sapiens(human)
Product mitochondrial dynamics protein MID49 isoform 3
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB183618.1, AK128310.1, AC127537.8 and BC014973.1. Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region that causes a translational frameshift, compared to variant 1. The resulting isoform (3) contains a distinct C-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK128310.1, DA153706.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149004, SAMEA2152719 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
ATGGCAGAGT TCTCCCAGAA ACGGGGGAAG CGGCGTAGCG ACGAAGGGCT GGGCAGCATG 
GTGGACTTCC TCCTGGCCAA TGCCCGCCTG GTGCTGGGCG TGGGCGGGGC TGCTGTGCTG 
GGCATTGCCA CCCTGGCCGT GAAGCGGTTC ATTGACAGGG CCACTAGCCC GCGGGATGAG 
GATGACACCA AGGCAGACAG CTGGAAGGAA CTGAGCCTGC TCAAGGCCAC ACCACACCTG 
CAGCCCCGGC CTCCACCTGC TGCCCTTAGC CAGCCAGTGT TGCCCTTGGC CCCCTCGTCG 
TCTGCCCCAG GAGAGGCTGC TGGCCTTCGA GCGGGACCGT GTGACCATCC CAGCAGCCCA 
GGTGGCTTTG GCCAAACAGC TGGCTGGCGA CATCGCCCTG GAGCTGCAGG CCTACTTTCG 
GAGCAAGTTC CCGGAACTGC CCTTTGGGGC ATTCGTGCCT GGGGGGCCGC TCTACGACGG 
GCTGCAGGCG GGGGCTGCGG ACCATGTGCG TCTCCTGGTG CCACTGGTGC TGGAGCCGGG 
CCTGTGGAGC CTGGTGCCGG GCGTGGACAC TGTGGCGAGG GACCCTCGCT GCTGGGCCGT 
GCGCAGGACG CAGCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001138372.1
CDS212..829
Translation

Target ORF information:

RefSeq Version NM_001144900.1
Organism Homo sapiens(human)
Definition Homo sapiens mitochondrial elongation factor 2 (MIEF2), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001144900.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCAGAGT TCTCCCAGAA ACGGGGGAAG CGGCGTAGCG ACGAAGGGCT GGGCAGCATG 
GTGGACTTCC TCCTGGCCAA TGCCCGCCTG GTGCTGGGCG TGGGCGGGGC TGCTGTGCTG 
GGCATTGCCA CCCTGGCCGT GAAGCGGTTC ATTGACAGGG CCACTAGCCC GCGGGATGAG 
GATGACACCA AGGCAGACAG CTGGAAGGAA CTGAGCCTGC TCAAGGCCAC ACCACACCTG 
CAGCCCCGGC CTCCACCTGC TGCCCTTAGC CAGCCAGTGT TGCCCTTGGC CCCCTCGTCG 
TCTGCCCCAG GAGAGGCTGC TGGCCTTCGA GCGGGACCGT GTGACCATCC CAGCAGCCCA 
GGTGGCTTTG GCCAAACAGC TGGCTGGCGA CATCGCCCTG GAGCTGCAGG CCTACTTTCG 
GAGCAAGTTC CCGGAACTGC CCTTTGGGGC ATTCGTGCCT GGGGGGCCGC TCTACGACGG 
GCTGCAGGCG GGGGCTGCGG ACCATGTGCG TCTCCTGGTG CCACTGGTGC TGGAGCCGGG 
CCTGTGGAGC CTGGTGCCGG GCGTGGACAC TGTGGCGAGG GACCCTCGCT GCTGGGCCGT 
GCGCAGGACG CAGCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu11703
Clone ID Related Accession (Same CDS sequence) NM_148886.1
Accession Version NM_148886.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1398bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-19
Organism Homo sapiens(human)
Product mitochondrial dynamics protein MID49 isoform 2
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC014973.1 and AC127537.8. Transcript Variant: This variant (2) differs in the 5' UTR and uses a distinct translational start codon, compared to variant 1. The resulting isoform (2) is longer at the N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014973.1, BG762276.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145245, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGGGCTGA GTCCCAATTT AGACAGGCAG ACCATGGCAG AGTTCTCCCA GAAACGGGGG 
AAGCGGCGTA GCGACGAAGG GCTGGGCAGC ATGGTGGACT TCCTCCTGGC CAATGCCCGC 
CTGGTGCTGG GCGTGGGCGG GGCTGCTGTG CTGGGCATTG CCACCCTGGC CGTGAAGCGG 
TTCATTGACA GGGCCACTAG CCCGCGGGAT GAGGATGACA CCAAGGCAGA CAGCTGGAAG 
GAACTGAGCC TGCTCAAGGC CACACCACAC CTGCAGCCCC GGCCTCCACC TGCTGCCCTT 
AGCCAGCCAG TGTTGCCCTT GGCCCCCTCG TCGTCTGCCC CAGAAGGGCC TGCAGAAACT 
GATCCTGAGG TGACACCACA GCTCAGCTCC CCAGCACCGC TGTGTCTGAC ACTGCAGGAG 
AGGCTGCTGG CCTTCGAGCG GGACCGTGTG ACCATCCCAG CAGCCCAGGT GGCTTTGGCC 
AAACAGCTGG CTGGCGACAT CGCCCTGGAG CTGCAGGCCT ACTTTCGGAG CAAGTTCCCG 
GAACTGCCCT TTGGGGCATT CGTGCCTGGG GGGCCGCTCT ACGACGGGCT GCAGGCGGGG 
GCTGCGGACC ATGTGCGTCT CCTGGTGCCA CTGGTGCTGG AGCCGGGCCT GTGGAGCCTG 
GTGCCGGGCG TGGACACTGT GGCGAGGGAC CCTCGCTGCT GGGCCGTGCG CAGGACGCAG 
CTTGAGTTCT GCCCCCGTGG GAGCAGCCCC TGGGACCGCT TCCTGGTCGG GGGCTACCTC 
TCCTCCCGCG TCCTGCTGGA GCTACTCCGC AAGGCGCTGG CTGCTTCTGT CAACTGGCCG 
GCCATTGGCA GCCTTCTCGG GTGCCTGATC CGGCCCAGCA TGGCCTCGGA GGAGCTGCTG 
CTCGAGGTGC AGCACGAACG CCTGGAGCTC ACTGTGGCTG TGCTTGTGGC AGTCCCTGGG 
GTCGATGCTG ACGACCGCCT CCTCTTGGCC TGGCCCCTGG AGGGGCTGGC GGGGAACCTC 
TGGCTGCAGG ACCTGTATCC AGTGGAGGCT GCTAGGCTGC GAGCCCTGGA CGACCATGAC 
GCTGGGACTC GCCGGCGGCT GCTGCTGCTG CTGTGTGCTG TCTGCCGTGG TTGCTCGGCT 
CTGGGGCAGC TAGGCCGGGG TCACCTGACC CAGGTGGTCC TGCGTCTGGG GGAGGACAAC 
GTGGATTGGA CGGAGGAGGC CTTGGGTGAG CGCTTCCTGC AAGCCCTGGA GCTGCTCATC 
GGCAGCCTGG AGCAGGCCAG CCTGCCCTGC CACTTCAACC CCAGCGTGAA CCTCTTCAGC 
AGCTTGCGTG AGGAGGAGAT TGACGACATT GGCTATGCGC TATACAGTGG CCTACAGGAG 
CCCGAGGGGC TGCTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_683684.2
CDS27..1424
Translation

Target ORF information:

RefSeq Version NM_148886.1
Organism Homo sapiens(human)
Definition Homo sapiens mitochondrial elongation factor 2 (MIEF2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_148886.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGGGCTGA GTCCCAATTT AGACAGGCAG ACCATGGCAG AGTTCTCCCA GAAACGGGGG 
AAGCGGCGTA GCGACGAAGG GCTGGGCAGC ATGGTGGACT TCCTCCTGGC CAATGCCCGC 
CTGGTGCTGG GCGTGGGCGG GGCTGCTGTG CTGGGCATTG CCACCCTGGC CGTGAAGCGG 
TTCATTGACA GGGCCACTAG CCCGCGGGAT GAGGATGACA CCAAGGCAGA CAGCTGGAAG 
GAACTGAGCC TGCTCAAGGC CACACCACAC CTGCAGCCCC GGCCTCCACC TGCTGCCCTT 
AGCCAGCCAG TGTTGCCCTT GGCCCCCTCG TCGTCTGCCC CAGAAGGGCC TGCAGAAACT 
GATCCTGAGG TGACACCACA GCTCAGCTCC CCAGCACCGC TGTGTCTGAC ACTGCAGGAG 
AGGCTGCTGG CCTTCGAGCG GGACCGTGTG ACCATCCCAG CAGCCCAGGT GGCTTTGGCC 
AAACAGCTGG CTGGCGACAT CGCCCTGGAG CTGCAGGCCT ACTTTCGGAG CAAGTTCCCG 
GAACTGCCCT TTGGGGCATT CGTGCCTGGG GGGCCGCTCT ACGACGGGCT GCAGGCGGGG 
GCTGCGGACC ATGTGCGTCT CCTGGTGCCA CTGGTGCTGG AGCCGGGCCT GTGGAGCCTG 
GTGCCGGGCG TGGACACTGT GGCGAGGGAC CCTCGCTGCT GGGCCGTGCG CAGGACGCAG 
CTTGAGTTCT GCCCCCGTGG GAGCAGCCCC TGGGACCGCT TCCTGGTCGG GGGCTACCTC 
TCCTCCCGCG TCCTGCTGGA GCTACTCCGC AAGGCGCTGG CTGCTTCTGT CAACTGGCCG 
GCCATTGGCA GCCTTCTCGG GTGCCTGATC CGGCCCAGCA TGGCCTCGGA GGAGCTGCTG 
CTCGAGGTGC AGCACGAACG CCTGGAGCTC ACTGTGGCTG TGCTTGTGGC AGTCCCTGGG 
GTCGATGCTG ACGACCGCCT CCTCTTGGCC TGGCCCCTGG AGGGGCTGGC GGGGAACCTC 
TGGCTGCAGG ACCTGTATCC AGTGGAGGCT GCTAGGCTGC GAGCCCTGGA CGACCATGAC 
GCTGGGACTC GCCGGCGGCT GCTGCTGCTG CTGTGTGCTG TCTGCCGTGG TTGCTCGGCT 
CTGGGGCAGC TAGGCCGGGG TCACCTGACC CAGGTGGTCC TGCGTCTGGG GGAGGACAAC 
GTGGATTGGA CGGAGGAGGC CTTGGGTGAG CGCTTCCTGC AAGCCCTGGA GCTGCTCATC 
GGCAGCCTGG AGCAGGCCAG CCTGCCCTGC CACTTCAACC CCAGCGTGAA CCTCTTCAGC 
AGCTTGCGTG AGGAGGAGAT TGACGACATT GGCTATGCGC TATACAGTGG CCTACAGGAG 
CCCGAGGGGC TGCTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu86598
Clone ID Related Accession (Same CDS sequence) XM_017024190.1
Accession Version XM_017024190.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1386bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-06-12
Organism Homo sapiens(human)
Product mitochondrial dynamics protein MID49 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCCATCTT ACAGGCAGAC CATGGCAGAG TTCTCCCAGA AACGGGGGAA GCGGCGTAGC 
GACGAAGGGC TGGGCAGCAT GGTGGACTTC CTCCTGGCCA ATGCCCGCCT GGTGCTGGGC 
GTGGGCGGGG CTGCTGTGCT GGGCATTGCC ACCCTGGCCG TGAAGCGGTT CATTGACAGG 
GCCACTAGCC CGCGGGATGA GGATGACACC AAGGCAGACA GCTGGAAGGA ACTGAGCCTG 
CTCAAGGCCA CACCACACCT GCAGCCCCGG CCTCCACCTG CTGCCCTTAG CCAGCCAGTG 
TTGCCCTTGG CCCCCTCGTC GTCTGCCCCA GAAGGGCCTG CAGAAACTGA TCCTGAGGTG 
ACACCACAGC TCAGCTCCCC AGCACCGCTG TGTCTGACAC TGCAGGAGAG GCTGCTGGCC 
TTCGAGCGGG ACCGTGTGAC CATCCCAGCA GCCCAGGTGG CTTTGGCCAA ACAGCTGGCT 
GGCGACATCG CCCTGGAGCT GCAGGCCTAC TTTCGGAGCA AGTTCCCGGA ACTGCCCTTT 
GGGGCATTCG TGCCTGGGGG GCCGCTCTAC GACGGGCTGC AGGCGGGGGC TGCGGACCAT 
GTGCGTCTCC TGGTGCCACT GGTGCTGGAG CCGGGCCTGT GGAGCCTGGT GCCGGGCGTG 
GACACTGTGG CGAGGGACCC TCGCTGCTGG GCCGTGCGCA GGACGCAGCT TGAGTTCTGC 
CCCCGTGGGA GCAGCCCCTG GGACCGCTTC CTGGTCGGGG GCTACCTCTC CTCCCGCGTC 
CTGCTGGAGC TACTCCGCAA GGCGCTGGCT GCTTCTGTCA ACTGGCCGGC CATTGGCAGC 
CTTCTCGGGT GCCTGATCCG GCCCAGCATG GCCTCGGAGG AGCTGCTGCT CGAGGTGCAG 
CACGAACGCC TGGAGCTCAC TGTGGCTGTG CTTGTGGCAG TCCCTGGGGT CGATGCTGAC 
GACCGCCTCC TCTTGGCCTG GCCCCTGGAG GGGCTGGCGG GGAACCTCTG GCTGCAGGAC 
CTGTATCCAG TGGAGGCTGC TAGGCTGCGA GCCCTGGACG ACCATGACGC TGGGACTCGC 
CGGCGGCTGC TGCTGCTGCT GTGTGCTGTC TGCCGTGGTT GCTCGGCTCT GGGGCAGCTA 
GGCCGGGGTC ACCTGACCCA GGTGGTCCTG CGTCTGGGGG AGGACAACGT GGATTGGACG 
GAGGAGGCCT TGGGTGAGCG CTTCCTGCAA GCCCTGGAGC TGCTCATCGG CAGCCTGGAG 
CAGGCCAGCC TGCCCTGCCA CTTCAACCCC AGCGTGAACC TCTTCAGCAG CTTGCGTGAG 
GAGGAGATTG ACGACATTGG CTATGCGCTA TACAGTGGCC TACAGGAGCC CGAGGGGCTG 
CTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016879679.1
CDS536..1921
Translation

Target ORF information:

RefSeq Version XM_017024190.1
Organism Homo sapiens(human)
Definition Homo sapiens mitochondrial elongation factor 2 (MIEF2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017024190.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCCATCTT ACAGGCAGAC CATGGCAGAG TTCTCCCAGA AACGGGGGAA GCGGCGTAGC 
GACGAAGGGC TGGGCAGCAT GGTGGACTTC CTCCTGGCCA ATGCCCGCCT GGTGCTGGGC 
GTGGGCGGGG CTGCTGTGCT GGGCATTGCC ACCCTGGCCG TGAAGCGGTT CATTGACAGG 
GCCACTAGCC CGCGGGATGA GGATGACACC AAGGCAGACA GCTGGAAGGA ACTGAGCCTG 
CTCAAGGCCA CACCACACCT GCAGCCCCGG CCTCCACCTG CTGCCCTTAG CCAGCCAGTG 
TTGCCCTTGG CCCCCTCGTC GTCTGCCCCA GAAGGGCCTG CAGAAACTGA TCCTGAGGTG 
ACACCACAGC TCAGCTCCCC AGCACCGCTG TGTCTGACAC TGCAGGAGAG GCTGCTGGCC 
TTCGAGCGGG ACCGTGTGAC CATCCCAGCA GCCCAGGTGG CTTTGGCCAA ACAGCTGGCT 
GGCGACATCG CCCTGGAGCT GCAGGCCTAC TTTCGGAGCA AGTTCCCGGA ACTGCCCTTT 
GGGGCATTCG TGCCTGGGGG GCCGCTCTAC GACGGGCTGC AGGCGGGGGC TGCGGACCAT 
GTGCGTCTCC TGGTGCCACT GGTGCTGGAG CCGGGCCTGT GGAGCCTGGT GCCGGGCGTG 
GACACTGTGG CGAGGGACCC TCGCTGCTGG GCCGTGCGCA GGACGCAGCT TGAGTTCTGC 
CCCCGTGGGA GCAGCCCCTG GGACCGCTTC CTGGTCGGGG GCTACCTCTC CTCCCGCGTC 
CTGCTGGAGC TACTCCGCAA GGCGCTGGCT GCTTCTGTCA ACTGGCCGGC CATTGGCAGC 
CTTCTCGGGT GCCTGATCCG GCCCAGCATG GCCTCGGAGG AGCTGCTGCT CGAGGTGCAG 
CACGAACGCC TGGAGCTCAC TGTGGCTGTG CTTGTGGCAG TCCCTGGGGT CGATGCTGAC 
GACCGCCTCC TCTTGGCCTG GCCCCTGGAG GGGCTGGCGG GGAACCTCTG GCTGCAGGAC 
CTGTATCCAG TGGAGGCTGC TAGGCTGCGA GCCCTGGACG ACCATGACGC TGGGACTCGC 
CGGCGGCTGC TGCTGCTGCT GTGTGCTGTC TGCCGTGGTT GCTCGGCTCT GGGGCAGCTA 
GGCCGGGGTC ACCTGACCCA GGTGGTCCTG CGTCTGGGGG AGGACAACGT GGATTGGACG 
GAGGAGGCCT TGGGTGAGCG CTTCCTGCAA GCCCTGGAGC TGCTCATCGG CAGCCTGGAG 
CAGGCCAGCC TGCCCTGCCA CTTCAACCCC AGCGTGAACC TCTTCAGCAG CTTGCGTGAG 
GAGGAGATTG ACGACATTGG CTATGCGCTA TACAGTGGCC TACAGGAGCC CGAGGGGCTG 
CTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Foxo3a inhibits mitochondrial fission and protects against doxorubicin-induced cardiotoxicity by suppressing MIEF2.
Free radical biology & medicine104360-370(2017 Mar)
Zhou L,Li R,Liu C,Sun T,Htet Aung LH,Chen C,Gao J,Zhao Y,Wang K


book

The role of Drp1 adaptor proteins MiD49 and MiD51?in mitochondrial fission: implications for human disease.
Clinical science (London, England : 1979)130(21)1861-74(2016 Nov)
Atkins K,Dasgupta A,Chen KH,Mewburn J,Archer SL


book

Drp1-dependent mitochondrial fission via MiD49/51 is essential for apoptotic cristae remodeling.
The Journal of cell biology212(5)531-44(2016 Feb)
Otera H,Miyata N,Kuge O,Mihara K