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CYP4F22 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol CYP4F22
Entrez Gene ID 126410
Full Name cytochrome P450 family 4 subfamily F member 22
Synonyms ARCI5,INLNE,LI3
General protein information
Preferred Names

cytochrome P450 family 4 subfamily F member 22

Names

cytochrome P450 4F22
cytochrome P450, family 2, subfamily E, polypeptide 2 homolog
cytochrome P450, family 4, subfamily F, polypeptide 22
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

19

19p13.12
Summary This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This gene is part of a cluster of cytochrome P450 genes on chromosome 19 and encodes an enzyme thought to play a role in the 12(R)-lipoxygenase pathway. Mutations in this gene are the cause of ichthyosis lamellar type 3. [provided by RefSeq, Jul 2008].
Disorder MIM:

611495

Disorder Html: Ichthyosis, congenital, autosomal recessive 5, 604777 (3)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_011527693.1 XP_011525995.1 cytochrome P450 4F22 isoform X1
XM_011527692.2 XP_011525994.1 cytochrome P450 4F22 isoform X1
NM_173483.3 NP_775754.2 cytochrome P450 4F22
XM_011527692.1 XP_011525994.1 cytochrome P450 4F22 isoform X1
NM_173483.4 NP_775754.2 cytochrome P450 4F22
XM_011527693.2 XP_011525995.1 cytochrome P450 4F22 isoform X1

Pathways from BioSystems


Homologies


Rattus norvegicus (Norway rat) Cyp4f39 XP_006241154.1
Xenopus tropicalis (tropical clawed frog) cyp4f22 NP_001015810.1
Pan troglodytes (chimpanzee) LOC455798 XP_512456.2
Bos taurus (cattle) LOC100301224 XP_005208627.1
CYP4F22 XP_001111971.1
Mus musculus (house mouse) Cyp4f39 NP_796281.1
Arabidopsis thaliana (thale cress) CYP735A2 NP_176882.1
Xenopus tropicalis (tropical clawed frog) LOC548774 NP_001016020.1
Homo sapiens (human) CYP4F22 NP_775754.2
Canis lupus familiaris (dog) CYP4F22 XP_541984.2
Arabidopsis thaliana (thale cress) CYP735A1 NP_198661.1
Oryza sativa (rice) Os09g0403300 NP_001175823.1

Gene Ontology


Bibliography

Related articles in PubMed

Two missense mutations in CYP4F22 in autosomal recessive congenital ichthyosis.
Feng C, Wang H, Lee M, Zhao J, Lin Z, Yang Y
Clinical and experimental dermatology42(1)98-100(2017 Jan)

Two Cases of Autosomal Recessive Congenital Ichthyosis due to CYP4F22 Mutations: Expanding the Genotype of Self-Healing Collodion Baby.
Noguera-Morel L, Feito-Rodr?guez M, Maldonado-Cid P, Garc?a-Mi??ur S, Kamsteeg EJ, Gonz?lez-Sarmiento R, De Lucas-Laguna R, Hern?ndez-Mart?n A, Torrelo A
Pediatric dermatology33(2)e48-51(2016 Mar-Apr)

Lamellar ichthyosis in a collodion baby caused by CYP4F22 mutations in a non-consanguineous family outside the Mediterranean.
Sugiura K, Takeichi T, Tanahashi K, Ito Y, Kosho T, Saida K, Uhara H, Okuyama R, Akiyama M
Journal of dermatological science72(2)193-5(2013 Nov)

CYP4F22 is highly expressed at the site and timing of onset of keratinization during skin development.
Sasaki K, Akiyama M, Yanagi T, Sakai K, Miyamura Y, Sato M, Shimizu H
Journal of dermatological science65(2)156-8(2012 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We report two cases of Congenital Ichthyosiform Erythroderma showing homozygous mutations in the gene CYP4F22.
Title: Two Cases of Autosomal Recessive Congenital Ichthyosis due to CYP4F22 Mutations: Expanding the Genotype of Self-Healing Collodion Baby.

description of CYP4F22 mutations from a Japanese collodion baby with lamellar ichthyosis [case report]
Title: Lamellar ichthyosis in a collodion baby caused by CYP4F22 mutations in a non-consanguineous family outside the Mediterranean.

Letter: CYP4F22 is highly expressed at the site and timing of onset of keratinization during skin development.
Title: CYP4F22 is highly expressed at the site and timing of onset of keratinization during skin development.

REVIEW: genetic analyses have identified a wide spectrum of mutations in the CYP4V2gene from patients suffering from Bietti's crystalline corneoretinal dystrophy, and mutations in theCYP4F22 gene have been linked to lamellar ichthyosis
Title: Finding homes for orphan cytochrome P450s: CYP4V2 and CYP4F22 in disease states.

The following CYP4F22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CYP4F22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu18711 NM_173483.4
Latest version!		 Homo sapiens cytochrome P450 family 4 subfamily F member 22 (CYP4F22), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $219.50-$307.30
$439.00
OHu18711 XM_011527693.2
Latest version!		 Homo sapiens cytochrome P450 family 4 subfamily F member 22 (CYP4F22), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $219.50-$307.30
$439.00
OHu18711 XM_011527692.2
Latest version!		 Homo sapiens cytochrome P450 family 4 subfamily F member 22 (CYP4F22), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $219.50-$307.30
$439.00
View Old Accession Versions >>
OHu18711 XM_011527692.1 Homo sapiens cytochrome P450, family 4, subfamily F, polypeptide 22 (CYP4F22), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $219.50-$307.30
$439.00
OHu18711 NM_173483.3 Homo sapiens cytochrome P450 family 4 subfamily F member 22 (CYP4F22), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $219.50-$307.30
$439.00
OHu18711 XM_011527693.1 Homo sapiens cytochrome P450, family 4, subfamily F, polypeptide 22 (CYP4F22), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $219.50-$307.30
$439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu18711
Clone ID Related Accession (Same CDS sequence) XM_011527693.1 , XM_011527692.2 , NM_173483.3 , XM_011527692.1 , NM_173483.4 , XM_011527693.2
Accession Version NM_173483.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-10-01
Organism Homo sapiens(human)
Product cytochrome P450 4F22
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA874258.1, BC069351.1, AC011492.8 and AI341068.1. This sequence is a reference standard in the RefSeqGene project. On Oct 11, 2007 this sequence version replaced NM_173483.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK096820.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
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601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCTGCCCA TCACAGACCG CCTGCTGCAC CTCCTGGGGC TGGAGAAGAC GGCGTTCCGC 
ATATACGCGG TGTCCACCCT TCTCCTCTTC CTGCTCTTCT TCCTGTTCCG CCTGCTGCTG 
CGGTTCCTGA GGCTCTGCAG GAGCTTCTAC ATCACCTGCC GCCGGCTGCG CTGCTTCCCC 
CAGCCTCCCC GGCGCAACTG GCTGCTGGGC CACCTGGGCA TGTACCTTCC AAATGAGGCG 
GGCCTTCAAG ATGAGAAGAA GGTACTGGAC AACATGCACC ATGTACTCTT GGTATGGATG 
GGACCTGTCC TGCCGCTGTT GGTTCTGGTG CACCCTGATT ACATCAAACC CCTTTTGGGA 
GCCTCAGCTG CCATCGCCCC CAAGGATGAC CTCTTCTATG GCTTCCTAAA ACCTTGGCTA 
GGGGATGGGC TGCTGCTCAG CAAAGGTGAC AAGTGGAGCC GGCACCGTCG CCTGCTGACA 
CCCGCCTTCC ACTTTGACAT CCTGAAGCCT TACATGAAGA TCTTCAACCA GAGCGCTGAC 
ATTATGCATG CTAAATGGCG GCATCTGGCA GAGGGCTCAG CGGTCTCCCT TGATATGTTT 
GAGCATATCA GCCTCATGAC CCTGGACAGT CTTCAGAAAT GTGTCTTCAG CTACAACAGC 
AACTGCCAAG AGAAGATGAG TGATTATATC TCCGCTATCA TTGAACTGAG CGCTCTGTCT 
GTCCGGCGCC AGTATCGCTT GCACCACTAC CTCGACTTCA TTTACTACCG CTCGGCGGAT 
GGGCGGAGGT TCCGGCAGGC CTGTGACATG GTGCACCACT TCACCACTGA AGTCATCCAG 
GAACGGCGGC GGGCACTGCG TCAGCAGGGG GCCGAGGCCT GGCTTAAGGC CAAGCAGGGG 
AAGACCTTGG ACTTTATTGA TGTGCTGCTC CTGGCCAGGG ATGAAGATGG AAAGGAACTG 
TCAGACGAGG ATATCCGAGC CGAAGCAGAC ACCTTCATGT TTGAGGGTCA CGACACAACA 
TCCAGTGGGA TCTCTTGGAT GCTGTTCAAT TTGGCAAAGT ATCCGGAATA CCAGGAGAAA 
TGCCGAGAAG AGATTCAGGA AGTCATGAAA GGCCGGGAGC TGGAGGAGCT GGAGTGGGAC 
GATCTGACTC AGCTGCCCTT TACAACTATG TGCATTAAGG AGAGCCTGCG CCAGTACCCA 
CCTGTCACTC TTGTCTCTCG CCAATGCACG GAGGACATCA AGCTCCCAGA TGGGCGCATC 
ATCCCCAAAG GAATCATCTG CTTGGTCAGC ATCTATGGAA CCCACCACAA CCCCACAGTG 
TGGCCTGACT CCAAGGTGTA CAACCCCTAC CGCTTTGACC CGGACAACCC ACAGCAGCGC 
TCTCCACTGG CCTATGTGCC CTTCTCTGCA GGACCCAGGA ATTGCATCGG ACAGAGCTTC 
GCCATGGCCG AGTTGCGCGT GGTTGTGGCA CTAACACTGC TACGTTTCCG CCTGAGCGTG 
GACCGAACGC GCAAGGTGCG GCGGAAGCCG GAGCTCATAC TGCGCACGGA GAACGGGCTC 
TGGCTCAAGG TGGAGCCGCT GCCTCCGCGG GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_775754.2
CDS168..1763
Translation

Target ORF information:

RefSeq Version NM_173483.3
Organism Homo sapiens(human)
Definition Homo sapiens cytochrome P450 family 4 subfamily F member 22 (CYP4F22), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_173483.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCTGCCCA TCACAGACCG CCTGCTGCAC CTCCTGGGGC TGGAGAAGAC GGCGTTCCGC 
ATATACGCGG TGTCCACCCT TCTCCTCTTC CTGCTCTTCT TCCTGTTCCG CCTGCTGCTG 
CGGTTCCTGA GGCTCTGCAG GAGCTTCTAC ATCACCTGCC GCCGGCTGCG CTGCTTCCCC 
CAGCCTCCCC GGCGCAACTG GCTGCTGGGC CACCTGGGCA TGTACCTTCC AAATGAGGCG 
GGCCTTCAAG ATGAGAAGAA GGTACTGGAC AACATGCACC ATGTACTCTT GGTATGGATG 
GGACCTGTCC TGCCGCTGTT GGTTCTGGTG CACCCTGATT ACATCAAACC CCTTTTGGGA 
GCCTCAGCTG CCATCGCCCC CAAGGATGAC CTCTTCTATG GCTTCCTAAA ACCTTGGCTA 
GGGGATGGGC TGCTGCTCAG CAAAGGTGAC AAGTGGAGCC GGCACCGTCG CCTGCTGACA 
CCCGCCTTCC ACTTTGACAT CCTGAAGCCT TACATGAAGA TCTTCAACCA GAGCGCTGAC 
ATTATGCATG CTAAATGGCG GCATCTGGCA GAGGGCTCAG CGGTCTCCCT TGATATGTTT 
GAGCATATCA GCCTCATGAC CCTGGACAGT CTTCAGAAAT GTGTCTTCAG CTACAACAGC 
AACTGCCAAG AGAAGATGAG TGATTATATC TCCGCTATCA TTGAACTGAG CGCTCTGTCT 
GTCCGGCGCC AGTATCGCTT GCACCACTAC CTCGACTTCA TTTACTACCG CTCGGCGGAT 
GGGCGGAGGT TCCGGCAGGC CTGTGACATG GTGCACCACT TCACCACTGA AGTCATCCAG 
GAACGGCGGC GGGCACTGCG TCAGCAGGGG GCCGAGGCCT GGCTTAAGGC CAAGCAGGGG 
AAGACCTTGG ACTTTATTGA TGTGCTGCTC CTGGCCAGGG ATGAAGATGG AAAGGAACTG 
TCAGACGAGG ATATCCGAGC CGAAGCAGAC ACCTTCATGT TTGAGGGTCA CGACACAACA 
TCCAGTGGGA TCTCTTGGAT GCTGTTCAAT TTGGCAAAGT ATCCGGAATA CCAGGAGAAA 
TGCCGAGAAG AGATTCAGGA AGTCATGAAA GGCCGGGAGC TGGAGGAGCT GGAGTGGGAC 
GATCTGACTC AGCTGCCCTT TACAACTATG TGCATTAAGG AGAGCCTGCG CCAGTACCCA 
CCTGTCACTC TTGTCTCTCG CCAATGCACG GAGGACATCA AGCTCCCAGA TGGGCGCATC 
ATCCCCAAAG GAATCATCTG CTTGGTCAGC ATCTATGGAA CCCACCACAA CCCCACAGTG 
TGGCCTGACT CCAAGGTGTA CAACCCCTAC CGCTTTGACC CGGACAACCC ACAGCAGCGC 
TCTCCACTGG CCTATGTGCC CTTCTCTGCA GGACCCAGGA ATTGCATCGG ACAGAGCTTC 
GCCATGGCCG AGTTGCGCGT GGTTGTGGCA CTAACACTGC TACGTTTCCG CCTGAGCGTG 
GACCGAACGC GCAAGGTGCG GCGGAAGCCG GAGCTCATAC TGCGCACGGA GAACGGGCTC 
TGGCTCAAGG TGGAGCCGCT GCCTCCGCGG GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18711
Clone ID Related Accession (Same CDS sequence) XM_011527693.1 , XM_011527692.2 , NM_173483.3 , XM_011527692.1 , NM_173483.4 , XM_011527693.2
Accession Version NM_173483.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2020-01-01
Organism Homo sapiens(human)
Product cytochrome P450 4F22
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA874258.1, BC069351.1, AC011492.8 and AI341068.1. This sequence is a reference standard in the RefSeqGene project. On Nov 24, 2018 this sequence version replaced NM_173483.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK096820.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000269703.8/ ENSP00000269703.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCTGCCCA TCACAGACCG CCTGCTGCAC CTCCTGGGGC TGGAGAAGAC GGCGTTCCGC 
ATATACGCGG TGTCCACCCT TCTCCTCTTC CTGCTCTTCT TCCTGTTCCG CCTGCTGCTG 
CGGTTCCTGA GGCTCTGCAG GAGCTTCTAC ATCACCTGCC GCCGGCTGCG CTGCTTCCCC 
CAGCCTCCCC GGCGCAACTG GCTGCTGGGC CACCTGGGCA TGTACCTTCC AAATGAGGCG 
GGCCTTCAAG ATGAGAAGAA GGTACTGGAC AACATGCACC ATGTACTCTT GGTATGGATG 
GGACCTGTCC TGCCGCTGTT GGTTCTGGTG CACCCTGATT ACATCAAACC CCTTTTGGGA 
GCCTCAGCTG CCATCGCCCC CAAGGATGAC CTCTTCTATG GCTTCCTAAA ACCTTGGCTA 
GGGGATGGGC TGCTGCTCAG CAAAGGTGAC AAGTGGAGCC GGCACCGTCG CCTGCTGACA 
CCCGCCTTCC ACTTTGACAT CCTGAAGCCT TACATGAAGA TCTTCAACCA GAGCGCTGAC 
ATTATGCATG CTAAATGGCG GCATCTGGCA GAGGGCTCAG CGGTCTCCCT TGATATGTTT 
GAGCATATCA GCCTCATGAC CCTGGACAGT CTTCAGAAAT GTGTCTTCAG CTACAACAGC 
AACTGCCAAG AGAAGATGAG TGATTATATC TCCGCTATCA TTGAACTGAG CGCTCTGTCT 
GTCCGGCGCC AGTATCGCTT GCACCACTAC CTCGACTTCA TTTACTACCG CTCGGCGGAT 
GGGCGGAGGT TCCGGCAGGC CTGTGACATG GTGCACCACT TCACCACTGA AGTCATCCAG 
GAACGGCGGC GGGCACTGCG TCAGCAGGGG GCCGAGGCCT GGCTTAAGGC CAAGCAGGGG 
AAGACCTTGG ACTTTATTGA TGTGCTGCTC CTGGCCAGGG ATGAAGATGG AAAGGAACTG 
TCAGACGAGG ATATCCGAGC CGAAGCAGAC ACCTTCATGT TTGAGGGTCA CGACACAACA 
TCCAGTGGGA TCTCTTGGAT GCTGTTCAAT TTGGCAAAGT ATCCGGAATA CCAGGAGAAA 
TGCCGAGAAG AGATTCAGGA AGTCATGAAA GGCCGGGAGC TGGAGGAGCT GGAGTGGGAC 
GATCTGACTC AGCTGCCCTT TACAACTATG TGCATTAAGG AGAGCCTGCG CCAGTACCCA 
CCTGTCACTC TTGTCTCTCG CCAATGCACG GAGGACATCA AGCTCCCAGA TGGGCGCATC 
ATCCCCAAAG GAATCATCTG CTTGGTCAGC ATCTATGGAA CCCACCACAA CCCCACAGTG 
TGGCCTGACT CCAAGGTGTA CAACCCCTAC CGCTTTGACC CGGACAACCC ACAGCAGCGC 
TCTCCACTGG CCTATGTGCC CTTCTCTGCA GGACCCAGGA ATTGCATCGG ACAGAGCTTC 
GCCATGGCCG AGTTGCGCGT GGTTGTGGCA CTAACACTGC TACGTTTCCG CCTGAGCGTG 
GACCGAACGC GCAAGGTGCG GCGGAAGCCG GAGCTCATAC TGCGCACGGA GAACGGGCTC 
TGGCTCAAGG TGGAGCCGCT GCCTCCGCGG GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_775754.2
CDS168..1763
Translation

Target ORF information:

RefSeq Version NM_173483.4
Organism Homo sapiens(human)
Definition Homo sapiens cytochrome P450 family 4 subfamily F member 22 (CYP4F22), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_173483.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCTGCCCA TCACAGACCG CCTGCTGCAC CTCCTGGGGC TGGAGAAGAC GGCGTTCCGC 
ATATACGCGG TGTCCACCCT TCTCCTCTTC CTGCTCTTCT TCCTGTTCCG CCTGCTGCTG 
CGGTTCCTGA GGCTCTGCAG GAGCTTCTAC ATCACCTGCC GCCGGCTGCG CTGCTTCCCC 
CAGCCTCCCC GGCGCAACTG GCTGCTGGGC CACCTGGGCA TGTACCTTCC AAATGAGGCG 
GGCCTTCAAG ATGAGAAGAA GGTACTGGAC AACATGCACC ATGTACTCTT GGTATGGATG 
GGACCTGTCC TGCCGCTGTT GGTTCTGGTG CACCCTGATT ACATCAAACC CCTTTTGGGA 
GCCTCAGCTG CCATCGCCCC CAAGGATGAC CTCTTCTATG GCTTCCTAAA ACCTTGGCTA 
GGGGATGGGC TGCTGCTCAG CAAAGGTGAC AAGTGGAGCC GGCACCGTCG CCTGCTGACA 
CCCGCCTTCC ACTTTGACAT CCTGAAGCCT TACATGAAGA TCTTCAACCA GAGCGCTGAC 
ATTATGCATG CTAAATGGCG GCATCTGGCA GAGGGCTCAG CGGTCTCCCT TGATATGTTT 
GAGCATATCA GCCTCATGAC CCTGGACAGT CTTCAGAAAT GTGTCTTCAG CTACAACAGC 
AACTGCCAAG AGAAGATGAG TGATTATATC TCCGCTATCA TTGAACTGAG CGCTCTGTCT 
GTCCGGCGCC AGTATCGCTT GCACCACTAC CTCGACTTCA TTTACTACCG CTCGGCGGAT 
GGGCGGAGGT TCCGGCAGGC CTGTGACATG GTGCACCACT TCACCACTGA AGTCATCCAG 
GAACGGCGGC GGGCACTGCG TCAGCAGGGG GCCGAGGCCT GGCTTAAGGC CAAGCAGGGG 
AAGACCTTGG ACTTTATTGA TGTGCTGCTC CTGGCCAGGG ATGAAGATGG AAAGGAACTG 
TCAGACGAGG ATATCCGAGC CGAAGCAGAC ACCTTCATGT TTGAGGGTCA CGACACAACA 
TCCAGTGGGA TCTCTTGGAT GCTGTTCAAT TTGGCAAAGT ATCCGGAATA CCAGGAGAAA 
TGCCGAGAAG AGATTCAGGA AGTCATGAAA GGCCGGGAGC TGGAGGAGCT GGAGTGGGAC 
GATCTGACTC AGCTGCCCTT TACAACTATG TGCATTAAGG AGAGCCTGCG CCAGTACCCA 
CCTGTCACTC TTGTCTCTCG CCAATGCACG GAGGACATCA AGCTCCCAGA TGGGCGCATC 
ATCCCCAAAG GAATCATCTG CTTGGTCAGC ATCTATGGAA CCCACCACAA CCCCACAGTG 
TGGCCTGACT CCAAGGTGTA CAACCCCTAC CGCTTTGACC CGGACAACCC ACAGCAGCGC 
TCTCCACTGG CCTATGTGCC CTTCTCTGCA GGACCCAGGA ATTGCATCGG ACAGAGCTTC 
GCCATGGCCG AGTTGCGCGT GGTTGTGGCA CTAACACTGC TACGTTTCCG CCTGAGCGTG 
GACCGAACGC GCAAGGTGCG GCGGAAGCCG GAGCTCATAC TGCGCACGGA GAACGGGCTC 
TGGCTCAAGG TGGAGCCGCT GCCTCCGCGG GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18711
Clone ID Related Accession (Same CDS sequence) XM_011527693.1 , XM_011527692.2 , NM_173483.3 , XM_011527692.1 , NM_173483.4 , XM_011527693.2
Accession Version XM_011527693.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-03-11
Organism Homo sapiens(Human)
Product cytochrome P450 4F22 isoform X1
Comment 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCTGCCCA TCACAGACCG CCTGCTGCAC CTCCTGGGGC TGGAGAAGAC GGCGTTCCGC 
ATATACGCGG TGTCCACCCT TCTCCTCTTC CTGCTCTTCT TCCTGTTCCG CCTGCTGCTG 
CGGTTCCTGA GGCTCTGCAG GAGCTTCTAC ATCACCTGCC GCCGGCTGCG CTGCTTCCCC 
CAGCCTCCCC GGCGCAACTG GCTGCTGGGC CACCTGGGCA TGTACCTTCC AAATGAGGCG 
GGCCTTCAAG ATGAGAAGAA GGTACTGGAC AACATGCACC ATGTACTCTT GGTATGGATG 
GGACCTGTCC TGCCGCTGTT GGTTCTGGTG CACCCTGATT ACATCAAACC CCTTTTGGGA 
GCCTCAGCTG CCATCGCCCC CAAGGATGAC CTCTTCTATG GCTTCCTAAA ACCTTGGCTA 
GGGGATGGGC TGCTGCTCAG CAAAGGTGAC AAGTGGAGCC GGCACCGTCG CCTGCTGACA 
CCCGCCTTCC ACTTTGACAT CCTGAAGCCT TACATGAAGA TCTTCAACCA GAGCGCTGAC 
ATTATGCATG CTAAATGGCG GCATCTGGCA GAGGGCTCAG CGGTCTCCCT TGATATGTTT 
GAGCATATCA GCCTCATGAC CCTGGACAGT CTTCAGAAAT GTGTCTTCAG CTACAACAGC 
AACTGCCAAG AGAAGATGAG TGATTATATC TCCGCTATCA TTGAACTGAG CGCTCTGTCT 
GTCCGGCGCC AGTATCGCTT GCACCACTAC CTCGACTTCA TTTACTACCG CTCGGCGGAT 
GGGCGGAGGT TCCGGCAGGC CTGTGACATG GTGCACCACT TCACCACTGA AGTCATCCAG 
GAACGGCGGC GGGCACTGCG TCAGCAGGGG GCCGAGGCCT GGCTTAAGGC CAAGCAGGGG 
AAGACCTTGG ACTTTATTGA TGTGCTGCTC CTGGCCAGGG ATGAAGATGG AAAGGAACTG 
TCAGACGAGG ATATCCGAGC CGAAGCAGAC ACCTTCATGT TTGAGGGTCA CGACACAACA 
TCCAGTGGGA TCTCTTGGAT GCTGTTCAAT TTGGCAAAGT ATCCGGAATA CCAGGAGAAA 
TGCCGAGAAG AGATTCAGGA AGTCATGAAA GGCCGGGAGC TGGAGGAGCT GGAGTGGGAC 
GATCTGACTC AGCTGCCCTT TACAACTATG TGCATTAAGG AGAGCCTGCG CCAGTACCCA 
CCTGTCACTC TTGTCTCTCG CCAATGCACG GAGGACATCA AGCTCCCAGA TGGGCGCATC 
ATCCCCAAAG GAATCATCTG CTTGGTCAGC ATCTATGGAA CCCACCACAA CCCCACAGTG 
TGGCCTGACT CCAAGGTGTA CAACCCCTAC CGCTTTGACC CGGACAACCC ACAGCAGCGC 
TCTCCACTGG CCTATGTGCC CTTCTCTGCA GGACCCAGGA ATTGCATCGG ACAGAGCTTC 
GCCATGGCCG AGTTGCGCGT GGTTGTGGCA CTAACACTGC TACGTTTCCG CCTGAGCGTG 
GACCGAACGC GCAAGGTGCG GCGGAAGCCG GAGCTCATAC TGCGCACGGA GAACGGGCTC 
TGGCTCAAGG TGGAGCCGCT GCCTCCGCGG GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011525995.1
Translation

Target ORF information:

RefSeq Version XM_011527693.1
Organism Homo sapiens(Human)
Definition Homo sapiens cytochrome P450, family 4, subfamily F, polypeptide 22 (CYP4F22), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011527693.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCTGCCCA TCACAGACCG CCTGCTGCAC CTCCTGGGGC TGGAGAAGAC GGCGTTCCGC 
ATATACGCGG TGTCCACCCT TCTCCTCTTC CTGCTCTTCT TCCTGTTCCG CCTGCTGCTG 
CGGTTCCTGA GGCTCTGCAG GAGCTTCTAC ATCACCTGCC GCCGGCTGCG CTGCTTCCCC 
CAGCCTCCCC GGCGCAACTG GCTGCTGGGC CACCTGGGCA TGTACCTTCC AAATGAGGCG 
GGCCTTCAAG ATGAGAAGAA GGTACTGGAC AACATGCACC ATGTACTCTT GGTATGGATG 
GGACCTGTCC TGCCGCTGTT GGTTCTGGTG CACCCTGATT ACATCAAACC CCTTTTGGGA 
GCCTCAGCTG CCATCGCCCC CAAGGATGAC CTCTTCTATG GCTTCCTAAA ACCTTGGCTA 
GGGGATGGGC TGCTGCTCAG CAAAGGTGAC AAGTGGAGCC GGCACCGTCG CCTGCTGACA 
CCCGCCTTCC ACTTTGACAT CCTGAAGCCT TACATGAAGA TCTTCAACCA GAGCGCTGAC 
ATTATGCATG CTAAATGGCG GCATCTGGCA GAGGGCTCAG CGGTCTCCCT TGATATGTTT 
GAGCATATCA GCCTCATGAC CCTGGACAGT CTTCAGAAAT GTGTCTTCAG CTACAACAGC 
AACTGCCAAG AGAAGATGAG TGATTATATC TCCGCTATCA TTGAACTGAG CGCTCTGTCT 
GTCCGGCGCC AGTATCGCTT GCACCACTAC CTCGACTTCA TTTACTACCG CTCGGCGGAT 
GGGCGGAGGT TCCGGCAGGC CTGTGACATG GTGCACCACT TCACCACTGA AGTCATCCAG 
GAACGGCGGC GGGCACTGCG TCAGCAGGGG GCCGAGGCCT GGCTTAAGGC CAAGCAGGGG 
AAGACCTTGG ACTTTATTGA TGTGCTGCTC CTGGCCAGGG ATGAAGATGG AAAGGAACTG 
TCAGACGAGG ATATCCGAGC CGAAGCAGAC ACCTTCATGT TTGAGGGTCA CGACACAACA 
TCCAGTGGGA TCTCTTGGAT GCTGTTCAAT TTGGCAAAGT ATCCGGAATA CCAGGAGAAA 
TGCCGAGAAG AGATTCAGGA AGTCATGAAA GGCCGGGAGC TGGAGGAGCT GGAGTGGGAC 
GATCTGACTC AGCTGCCCTT TACAACTATG TGCATTAAGG AGAGCCTGCG CCAGTACCCA 
CCTGTCACTC TTGTCTCTCG CCAATGCACG GAGGACATCA AGCTCCCAGA TGGGCGCATC 
ATCCCCAAAG GAATCATCTG CTTGGTCAGC ATCTATGGAA CCCACCACAA CCCCACAGTG 
TGGCCTGACT CCAAGGTGTA CAACCCCTAC CGCTTTGACC CGGACAACCC ACAGCAGCGC 
TCTCCACTGG CCTATGTGCC CTTCTCTGCA GGACCCAGGA ATTGCATCGG ACAGAGCTTC 
GCCATGGCCG AGTTGCGCGT GGTTGTGGCA CTAACACTGC TACGTTTCCG CCTGAGCGTG 
GACCGAACGC GCAAGGTGCG GCGGAAGCCG GAGCTCATAC TGCGCACGGA GAACGGGCTC 
TGGCTCAAGG TGGAGCCGCT GCCTCCGCGG GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18711
Clone ID Related Accession (Same CDS sequence) XM_011527693.1 , XM_011527692.2 , NM_173483.3 , XM_011527692.1 , NM_173483.4 , XM_011527693.2
Accession Version XM_011527692.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product cytochrome P450 4F22 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527692.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCTGCCCA TCACAGACCG CCTGCTGCAC CTCCTGGGGC TGGAGAAGAC GGCGTTCCGC 
ATATACGCGG TGTCCACCCT TCTCCTCTTC CTGCTCTTCT TCCTGTTCCG CCTGCTGCTG 
CGGTTCCTGA GGCTCTGCAG GAGCTTCTAC ATCACCTGCC GCCGGCTGCG CTGCTTCCCC 
CAGCCTCCCC GGCGCAACTG GCTGCTGGGC CACCTGGGCA TGTACCTTCC AAATGAGGCG 
GGCCTTCAAG ATGAGAAGAA GGTACTGGAC AACATGCACC ATGTACTCTT GGTATGGATG 
GGACCTGTCC TGCCGCTGTT GGTTCTGGTG CACCCTGATT ACATCAAACC CCTTTTGGGA 
GCCTCAGCTG CCATCGCCCC CAAGGATGAC CTCTTCTATG GCTTCCTAAA ACCTTGGCTA 
GGGGATGGGC TGCTGCTCAG CAAAGGTGAC AAGTGGAGCC GGCACCGTCG CCTGCTGACA 
CCCGCCTTCC ACTTTGACAT CCTGAAGCCT TACATGAAGA TCTTCAACCA GAGCGCTGAC 
ATTATGCATG CTAAATGGCG GCATCTGGCA GAGGGCTCAG CGGTCTCCCT TGATATGTTT 
GAGCATATCA GCCTCATGAC CCTGGACAGT CTTCAGAAAT GTGTCTTCAG CTACAACAGC 
AACTGCCAAG AGAAGATGAG TGATTATATC TCCGCTATCA TTGAACTGAG CGCTCTGTCT 
GTCCGGCGCC AGTATCGCTT GCACCACTAC CTCGACTTCA TTTACTACCG CTCGGCGGAT 
GGGCGGAGGT TCCGGCAGGC CTGTGACATG GTGCACCACT TCACCACTGA AGTCATCCAG 
GAACGGCGGC GGGCACTGCG TCAGCAGGGG GCCGAGGCCT GGCTTAAGGC CAAGCAGGGG 
AAGACCTTGG ACTTTATTGA TGTGCTGCTC CTGGCCAGGG ATGAAGATGG AAAGGAACTG 
TCAGACGAGG ATATCCGAGC CGAAGCAGAC ACCTTCATGT TTGAGGGTCA CGACACAACA 
TCCAGTGGGA TCTCTTGGAT GCTGTTCAAT TTGGCAAAGT ATCCGGAATA CCAGGAGAAA 
TGCCGAGAAG AGATTCAGGA AGTCATGAAA GGCCGGGAGC TGGAGGAGCT GGAGTGGGAC 
GATCTGACTC AGCTGCCCTT TACAACTATG TGCATTAAGG AGAGCCTGCG CCAGTACCCA 
CCTGTCACTC TTGTCTCTCG CCAATGCACG GAGGACATCA AGCTCCCAGA TGGGCGCATC 
ATCCCCAAAG GAATCATCTG CTTGGTCAGC ATCTATGGAA CCCACCACAA CCCCACAGTG 
TGGCCTGACT CCAAGGTGTA CAACCCCTAC CGCTTTGACC CGGACAACCC ACAGCAGCGC 
TCTCCACTGG CCTATGTGCC CTTCTCTGCA GGACCCAGGA ATTGCATCGG ACAGAGCTTC 
GCCATGGCCG AGTTGCGCGT GGTTGTGGCA CTAACACTGC TACGTTTCCG CCTGAGCGTG 
GACCGAACGC GCAAGGTGCG GCGGAAGCCG GAGCTCATAC TGCGCACGGA GAACGGGCTC 
TGGCTCAAGG TGGAGCCGCT GCCTCCGCGG GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011525994.1
CDS410..2005
Translation

Target ORF information:

RefSeq Version XM_011527692.2
Organism Homo sapiens(human)
Definition Homo sapiens cytochrome P450 family 4 subfamily F member 22 (CYP4F22), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011527692.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCTGCCCA TCACAGACCG CCTGCTGCAC CTCCTGGGGC TGGAGAAGAC GGCGTTCCGC 
ATATACGCGG TGTCCACCCT TCTCCTCTTC CTGCTCTTCT TCCTGTTCCG CCTGCTGCTG 
CGGTTCCTGA GGCTCTGCAG GAGCTTCTAC ATCACCTGCC GCCGGCTGCG CTGCTTCCCC 
CAGCCTCCCC GGCGCAACTG GCTGCTGGGC CACCTGGGCA TGTACCTTCC AAATGAGGCG 
GGCCTTCAAG ATGAGAAGAA GGTACTGGAC AACATGCACC ATGTACTCTT GGTATGGATG 
GGACCTGTCC TGCCGCTGTT GGTTCTGGTG CACCCTGATT ACATCAAACC CCTTTTGGGA 
GCCTCAGCTG CCATCGCCCC CAAGGATGAC CTCTTCTATG GCTTCCTAAA ACCTTGGCTA 
GGGGATGGGC TGCTGCTCAG CAAAGGTGAC AAGTGGAGCC GGCACCGTCG CCTGCTGACA 
CCCGCCTTCC ACTTTGACAT CCTGAAGCCT TACATGAAGA TCTTCAACCA GAGCGCTGAC 
ATTATGCATG CTAAATGGCG GCATCTGGCA GAGGGCTCAG CGGTCTCCCT TGATATGTTT 
GAGCATATCA GCCTCATGAC CCTGGACAGT CTTCAGAAAT GTGTCTTCAG CTACAACAGC 
AACTGCCAAG AGAAGATGAG TGATTATATC TCCGCTATCA TTGAACTGAG CGCTCTGTCT 
GTCCGGCGCC AGTATCGCTT GCACCACTAC CTCGACTTCA TTTACTACCG CTCGGCGGAT 
GGGCGGAGGT TCCGGCAGGC CTGTGACATG GTGCACCACT TCACCACTGA AGTCATCCAG 
GAACGGCGGC GGGCACTGCG TCAGCAGGGG GCCGAGGCCT GGCTTAAGGC CAAGCAGGGG 
AAGACCTTGG ACTTTATTGA TGTGCTGCTC CTGGCCAGGG ATGAAGATGG AAAGGAACTG 
TCAGACGAGG ATATCCGAGC CGAAGCAGAC ACCTTCATGT TTGAGGGTCA CGACACAACA 
TCCAGTGGGA TCTCTTGGAT GCTGTTCAAT TTGGCAAAGT ATCCGGAATA CCAGGAGAAA 
TGCCGAGAAG AGATTCAGGA AGTCATGAAA GGCCGGGAGC TGGAGGAGCT GGAGTGGGAC 
GATCTGACTC AGCTGCCCTT TACAACTATG TGCATTAAGG AGAGCCTGCG CCAGTACCCA 
CCTGTCACTC TTGTCTCTCG CCAATGCACG GAGGACATCA AGCTCCCAGA TGGGCGCATC 
ATCCCCAAAG GAATCATCTG CTTGGTCAGC ATCTATGGAA CCCACCACAA CCCCACAGTG 
TGGCCTGACT CCAAGGTGTA CAACCCCTAC CGCTTTGACC CGGACAACCC ACAGCAGCGC 
TCTCCACTGG CCTATGTGCC CTTCTCTGCA GGACCCAGGA ATTGCATCGG ACAGAGCTTC 
GCCATGGCCG AGTTGCGCGT GGTTGTGGCA CTAACACTGC TACGTTTCCG CCTGAGCGTG 
GACCGAACGC GCAAGGTGCG GCGGAAGCCG GAGCTCATAC TGCGCACGGA GAACGGGCTC 
TGGCTCAAGG TGGAGCCGCT GCCTCCGCGG GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18711
Clone ID Related Accession (Same CDS sequence) XM_011527693.1 , XM_011527692.2 , NM_173483.3 , XM_011527692.1 , NM_173483.4 , XM_011527693.2
Accession Version XM_011527692.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-03-11
Organism Homo sapiens(Human)
Product cytochrome P450 4F22 isoform X1
Comment 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCTGCCCA TCACAGACCG CCTGCTGCAC CTCCTGGGGC TGGAGAAGAC GGCGTTCCGC 
ATATACGCGG TGTCCACCCT TCTCCTCTTC CTGCTCTTCT TCCTGTTCCG CCTGCTGCTG 
CGGTTCCTGA GGCTCTGCAG GAGCTTCTAC ATCACCTGCC GCCGGCTGCG CTGCTTCCCC 
CAGCCTCCCC GGCGCAACTG GCTGCTGGGC CACCTGGGCA TGTACCTTCC AAATGAGGCG 
GGCCTTCAAG ATGAGAAGAA GGTACTGGAC AACATGCACC ATGTACTCTT GGTATGGATG 
GGACCTGTCC TGCCGCTGTT GGTTCTGGTG CACCCTGATT ACATCAAACC CCTTTTGGGA 
GCCTCAGCTG CCATCGCCCC CAAGGATGAC CTCTTCTATG GCTTCCTAAA ACCTTGGCTA 
GGGGATGGGC TGCTGCTCAG CAAAGGTGAC AAGTGGAGCC GGCACCGTCG CCTGCTGACA 
CCCGCCTTCC ACTTTGACAT CCTGAAGCCT TACATGAAGA TCTTCAACCA GAGCGCTGAC 
ATTATGCATG CTAAATGGCG GCATCTGGCA GAGGGCTCAG CGGTCTCCCT TGATATGTTT 
GAGCATATCA GCCTCATGAC CCTGGACAGT CTTCAGAAAT GTGTCTTCAG CTACAACAGC 
AACTGCCAAG AGAAGATGAG TGATTATATC TCCGCTATCA TTGAACTGAG CGCTCTGTCT 
GTCCGGCGCC AGTATCGCTT GCACCACTAC CTCGACTTCA TTTACTACCG CTCGGCGGAT 
GGGCGGAGGT TCCGGCAGGC CTGTGACATG GTGCACCACT TCACCACTGA AGTCATCCAG 
GAACGGCGGC GGGCACTGCG TCAGCAGGGG GCCGAGGCCT GGCTTAAGGC CAAGCAGGGG 
AAGACCTTGG ACTTTATTGA TGTGCTGCTC CTGGCCAGGG ATGAAGATGG AAAGGAACTG 
TCAGACGAGG ATATCCGAGC CGAAGCAGAC ACCTTCATGT TTGAGGGTCA CGACACAACA 
TCCAGTGGGA TCTCTTGGAT GCTGTTCAAT TTGGCAAAGT ATCCGGAATA CCAGGAGAAA 
TGCCGAGAAG AGATTCAGGA AGTCATGAAA GGCCGGGAGC TGGAGGAGCT GGAGTGGGAC 
GATCTGACTC AGCTGCCCTT TACAACTATG TGCATTAAGG AGAGCCTGCG CCAGTACCCA 
CCTGTCACTC TTGTCTCTCG CCAATGCACG GAGGACATCA AGCTCCCAGA TGGGCGCATC 
ATCCCCAAAG GAATCATCTG CTTGGTCAGC ATCTATGGAA CCCACCACAA CCCCACAGTG 
TGGCCTGACT CCAAGGTGTA CAACCCCTAC CGCTTTGACC CGGACAACCC ACAGCAGCGC 
TCTCCACTGG CCTATGTGCC CTTCTCTGCA GGACCCAGGA ATTGCATCGG ACAGAGCTTC 
GCCATGGCCG AGTTGCGCGT GGTTGTGGCA CTAACACTGC TACGTTTCCG CCTGAGCGTG 
GACCGAACGC GCAAGGTGCG GCGGAAGCCG GAGCTCATAC TGCGCACGGA GAACGGGCTC 
TGGCTCAAGG TGGAGCCGCT GCCTCCGCGG GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011525994.1
Translation

Target ORF information:

RefSeq Version XM_011527692.1
Organism Homo sapiens(Human)
Definition Homo sapiens cytochrome P450, family 4, subfamily F, polypeptide 22 (CYP4F22), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011527692.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCTGCCCA TCACAGACCG CCTGCTGCAC CTCCTGGGGC TGGAGAAGAC GGCGTTCCGC 
ATATACGCGG TGTCCACCCT TCTCCTCTTC CTGCTCTTCT TCCTGTTCCG CCTGCTGCTG 
CGGTTCCTGA GGCTCTGCAG GAGCTTCTAC ATCACCTGCC GCCGGCTGCG CTGCTTCCCC 
CAGCCTCCCC GGCGCAACTG GCTGCTGGGC CACCTGGGCA TGTACCTTCC AAATGAGGCG 
GGCCTTCAAG ATGAGAAGAA GGTACTGGAC AACATGCACC ATGTACTCTT GGTATGGATG 
GGACCTGTCC TGCCGCTGTT GGTTCTGGTG CACCCTGATT ACATCAAACC CCTTTTGGGA 
GCCTCAGCTG CCATCGCCCC CAAGGATGAC CTCTTCTATG GCTTCCTAAA ACCTTGGCTA 
GGGGATGGGC TGCTGCTCAG CAAAGGTGAC AAGTGGAGCC GGCACCGTCG CCTGCTGACA 
CCCGCCTTCC ACTTTGACAT CCTGAAGCCT TACATGAAGA TCTTCAACCA GAGCGCTGAC 
ATTATGCATG CTAAATGGCG GCATCTGGCA GAGGGCTCAG CGGTCTCCCT TGATATGTTT 
GAGCATATCA GCCTCATGAC CCTGGACAGT CTTCAGAAAT GTGTCTTCAG CTACAACAGC 
AACTGCCAAG AGAAGATGAG TGATTATATC TCCGCTATCA TTGAACTGAG CGCTCTGTCT 
GTCCGGCGCC AGTATCGCTT GCACCACTAC CTCGACTTCA TTTACTACCG CTCGGCGGAT 
GGGCGGAGGT TCCGGCAGGC CTGTGACATG GTGCACCACT TCACCACTGA AGTCATCCAG 
GAACGGCGGC GGGCACTGCG TCAGCAGGGG GCCGAGGCCT GGCTTAAGGC CAAGCAGGGG 
AAGACCTTGG ACTTTATTGA TGTGCTGCTC CTGGCCAGGG ATGAAGATGG AAAGGAACTG 
TCAGACGAGG ATATCCGAGC CGAAGCAGAC ACCTTCATGT TTGAGGGTCA CGACACAACA 
TCCAGTGGGA TCTCTTGGAT GCTGTTCAAT TTGGCAAAGT ATCCGGAATA CCAGGAGAAA 
TGCCGAGAAG AGATTCAGGA AGTCATGAAA GGCCGGGAGC TGGAGGAGCT GGAGTGGGAC 
GATCTGACTC AGCTGCCCTT TACAACTATG TGCATTAAGG AGAGCCTGCG CCAGTACCCA 
CCTGTCACTC TTGTCTCTCG CCAATGCACG GAGGACATCA AGCTCCCAGA TGGGCGCATC 
ATCCCCAAAG GAATCATCTG CTTGGTCAGC ATCTATGGAA CCCACCACAA CCCCACAGTG 
TGGCCTGACT CCAAGGTGTA CAACCCCTAC CGCTTTGACC CGGACAACCC ACAGCAGCGC 
TCTCCACTGG CCTATGTGCC CTTCTCTGCA GGACCCAGGA ATTGCATCGG ACAGAGCTTC 
GCCATGGCCG AGTTGCGCGT GGTTGTGGCA CTAACACTGC TACGTTTCCG CCTGAGCGTG 
GACCGAACGC GCAAGGTGCG GCGGAAGCCG GAGCTCATAC TGCGCACGGA GAACGGGCTC 
TGGCTCAAGG TGGAGCCGCT GCCTCCGCGG GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18711
Clone ID Related Accession (Same CDS sequence) XM_011527693.1 , XM_011527692.2 , NM_173483.3 , XM_011527692.1 , NM_173483.4 , XM_011527693.2
Accession Version XM_011527693.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product cytochrome P450 4F22 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527693.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCTGCCCA TCACAGACCG CCTGCTGCAC CTCCTGGGGC TGGAGAAGAC GGCGTTCCGC 
ATATACGCGG TGTCCACCCT TCTCCTCTTC CTGCTCTTCT TCCTGTTCCG CCTGCTGCTG 
CGGTTCCTGA GGCTCTGCAG GAGCTTCTAC ATCACCTGCC GCCGGCTGCG CTGCTTCCCC 
CAGCCTCCCC GGCGCAACTG GCTGCTGGGC CACCTGGGCA TGTACCTTCC AAATGAGGCG 
GGCCTTCAAG ATGAGAAGAA GGTACTGGAC AACATGCACC ATGTACTCTT GGTATGGATG 
GGACCTGTCC TGCCGCTGTT GGTTCTGGTG CACCCTGATT ACATCAAACC CCTTTTGGGA 
GCCTCAGCTG CCATCGCCCC CAAGGATGAC CTCTTCTATG GCTTCCTAAA ACCTTGGCTA 
GGGGATGGGC TGCTGCTCAG CAAAGGTGAC AAGTGGAGCC GGCACCGTCG CCTGCTGACA 
CCCGCCTTCC ACTTTGACAT CCTGAAGCCT TACATGAAGA TCTTCAACCA GAGCGCTGAC 
ATTATGCATG CTAAATGGCG GCATCTGGCA GAGGGCTCAG CGGTCTCCCT TGATATGTTT 
GAGCATATCA GCCTCATGAC CCTGGACAGT CTTCAGAAAT GTGTCTTCAG CTACAACAGC 
AACTGCCAAG AGAAGATGAG TGATTATATC TCCGCTATCA TTGAACTGAG CGCTCTGTCT 
GTCCGGCGCC AGTATCGCTT GCACCACTAC CTCGACTTCA TTTACTACCG CTCGGCGGAT 
GGGCGGAGGT TCCGGCAGGC CTGTGACATG GTGCACCACT TCACCACTGA AGTCATCCAG 
GAACGGCGGC GGGCACTGCG TCAGCAGGGG GCCGAGGCCT GGCTTAAGGC CAAGCAGGGG 
AAGACCTTGG ACTTTATTGA TGTGCTGCTC CTGGCCAGGG ATGAAGATGG AAAGGAACTG 
TCAGACGAGG ATATCCGAGC CGAAGCAGAC ACCTTCATGT TTGAGGGTCA CGACACAACA 
TCCAGTGGGA TCTCTTGGAT GCTGTTCAAT TTGGCAAAGT ATCCGGAATA CCAGGAGAAA 
TGCCGAGAAG AGATTCAGGA AGTCATGAAA GGCCGGGAGC TGGAGGAGCT GGAGTGGGAC 
GATCTGACTC AGCTGCCCTT TACAACTATG TGCATTAAGG AGAGCCTGCG CCAGTACCCA 
CCTGTCACTC TTGTCTCTCG CCAATGCACG GAGGACATCA AGCTCCCAGA TGGGCGCATC 
ATCCCCAAAG GAATCATCTG CTTGGTCAGC ATCTATGGAA CCCACCACAA CCCCACAGTG 
TGGCCTGACT CCAAGGTGTA CAACCCCTAC CGCTTTGACC CGGACAACCC ACAGCAGCGC 
TCTCCACTGG CCTATGTGCC CTTCTCTGCA GGACCCAGGA ATTGCATCGG ACAGAGCTTC 
GCCATGGCCG AGTTGCGCGT GGTTGTGGCA CTAACACTGC TACGTTTCCG CCTGAGCGTG 
GACCGAACGC GCAAGGTGCG GCGGAAGCCG GAGCTCATAC TGCGCACGGA GAACGGGCTC 
TGGCTCAAGG TGGAGCCGCT GCCTCCGCGG GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011525995.1
CDS180..1775
Translation

Target ORF information:

RefSeq Version XM_011527693.2
Organism Homo sapiens(human)
Definition Homo sapiens cytochrome P450 family 4 subfamily F member 22 (CYP4F22), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011527693.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCTGCCCA TCACAGACCG CCTGCTGCAC CTCCTGGGGC TGGAGAAGAC GGCGTTCCGC 
ATATACGCGG TGTCCACCCT TCTCCTCTTC CTGCTCTTCT TCCTGTTCCG CCTGCTGCTG 
CGGTTCCTGA GGCTCTGCAG GAGCTTCTAC ATCACCTGCC GCCGGCTGCG CTGCTTCCCC 
CAGCCTCCCC GGCGCAACTG GCTGCTGGGC CACCTGGGCA TGTACCTTCC AAATGAGGCG 
GGCCTTCAAG ATGAGAAGAA GGTACTGGAC AACATGCACC ATGTACTCTT GGTATGGATG 
GGACCTGTCC TGCCGCTGTT GGTTCTGGTG CACCCTGATT ACATCAAACC CCTTTTGGGA 
GCCTCAGCTG CCATCGCCCC CAAGGATGAC CTCTTCTATG GCTTCCTAAA ACCTTGGCTA 
GGGGATGGGC TGCTGCTCAG CAAAGGTGAC AAGTGGAGCC GGCACCGTCG CCTGCTGACA 
CCCGCCTTCC ACTTTGACAT CCTGAAGCCT TACATGAAGA TCTTCAACCA GAGCGCTGAC 
ATTATGCATG CTAAATGGCG GCATCTGGCA GAGGGCTCAG CGGTCTCCCT TGATATGTTT 
GAGCATATCA GCCTCATGAC CCTGGACAGT CTTCAGAAAT GTGTCTTCAG CTACAACAGC 
AACTGCCAAG AGAAGATGAG TGATTATATC TCCGCTATCA TTGAACTGAG CGCTCTGTCT 
GTCCGGCGCC AGTATCGCTT GCACCACTAC CTCGACTTCA TTTACTACCG CTCGGCGGAT 
GGGCGGAGGT TCCGGCAGGC CTGTGACATG GTGCACCACT TCACCACTGA AGTCATCCAG 
GAACGGCGGC GGGCACTGCG TCAGCAGGGG GCCGAGGCCT GGCTTAAGGC CAAGCAGGGG 
AAGACCTTGG ACTTTATTGA TGTGCTGCTC CTGGCCAGGG ATGAAGATGG AAAGGAACTG 
TCAGACGAGG ATATCCGAGC CGAAGCAGAC ACCTTCATGT TTGAGGGTCA CGACACAACA 
TCCAGTGGGA TCTCTTGGAT GCTGTTCAAT TTGGCAAAGT ATCCGGAATA CCAGGAGAAA 
TGCCGAGAAG AGATTCAGGA AGTCATGAAA GGCCGGGAGC TGGAGGAGCT GGAGTGGGAC 
GATCTGACTC AGCTGCCCTT TACAACTATG TGCATTAAGG AGAGCCTGCG CCAGTACCCA 
CCTGTCACTC TTGTCTCTCG CCAATGCACG GAGGACATCA AGCTCCCAGA TGGGCGCATC 
ATCCCCAAAG GAATCATCTG CTTGGTCAGC ATCTATGGAA CCCACCACAA CCCCACAGTG 
TGGCCTGACT CCAAGGTGTA CAACCCCTAC CGCTTTGACC CGGACAACCC ACAGCAGCGC 
TCTCCACTGG CCTATGTGCC CTTCTCTGCA GGACCCAGGA ATTGCATCGG ACAGAGCTTC 
GCCATGGCCG AGTTGCGCGT GGTTGTGGCA CTAACACTGC TACGTTTCCG CCTGAGCGTG 
GACCGAACGC GCAAGGTGCG GCGGAAGCCG GAGCTCATAC TGCGCACGGA GAACGGGCTC 
TGGCTCAAGG TGGAGCCGCT GCCTCCGCGG GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Two missense mutations in CYP4F22 in autosomal recessive congenital ichthyosis.
Clinical and experimental dermatology42(1)98-100(2017 Jan)
Feng C,Wang H,Lee M,Zhao J,Lin Z,Yang Y


book

Two Cases of Autosomal Recessive Congenital Ichthyosis due to CYP4F22 Mutations: Expanding the Genotype of Self-Healing Collodion Baby.
Pediatric dermatology33(2)e48-51(2016 Mar-Apr)
Noguera-Morel L,Feito-Rodr?guez M,Maldonado-Cid P,Garc?a-Mi??ur S,Kamsteeg EJ,Gonz?lez-Sarmiento R,De Lucas-Laguna R,Hern?ndez-Mart?n A,Torrelo A


book

Lamellar ichthyosis in a collodion baby caused by CYP4F22 mutations in a non-consanguineous family outside the Mediterranean.
Journal of dermatological science72(2)193-5(2013 Nov)
Sugiura K,Takeichi T,Tanahashi K,Ito Y,Kosho T,Saida K,Uhara H,Okuyama R,Akiyama M


book

CYP4F22 is highly expressed at the site and timing of onset of keratinization during skin development.
Journal of dermatological science65(2)156-8(2012 Feb)
Sasaki K,Akiyama M,Yanagi T,Sakai K,Miyamura Y,Sato M,Shimizu H