×

CYP4F22 cDNA ORF clone, Homo sapiens (human)

Summary

Gene Symbol CYP4F22
Entrez Gene ID 126410
Full Name cytochrome P450, family 4, subfamily F, polypeptide 22
Synonyms ARCI5, INLNE, LI3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

19

19p13.12
Summary This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This gene is part of a cluster of cytochrome P450 genes on chromosome 19 and encodes an enzyme thought to play a role in the 12(R)-lipoxygenase pathway. Mutations in this gene are the cause of ichthyosis lamellar type 3. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

611495

Disorder Html: Ichthyosis, lamellar, 3, 604777 (3)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_011527692 XP_011525994 cytochrome P450 4F22 isoform X1
XM_011527693 XP_011525995 cytochrome P450 4F22 isoform X1
NM_173483 NP_775754 cytochrome P450 4F22

Pathways from BioSystems


Homologies


Homo sapiens (human) CYP4F22 NP_775754.2
Pan troglodytes (chimpanzee) LOC455798 XP_512456.2
Macaca mulatta (Rhesus monkey) CYP4F22 XP_001111971.1
Canis lupus familiaris (dog) CYP4F22 XP_541984.2
Bos taurus (cattle) LOC100301224 XP_005208627.1
Mus musculus (house mouse) Cyp4f39 NP_796281.1
Rattus norvegicus (Norway rat) Cyp4f39 XP_006241154.1
Arabidopsis thaliana (thale cress) CYP735A2 NP_176882.1
Arabidopsis thaliana (thale cress) CYP735A1 NP_198661.1
Xenopus (Silurana) tropicalis (western clawed frog) cyp4f22 NP_001015810.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC548774 NP_001016020.1

Gene Ontology


Bibliography

Related articles in PubMed

  1. CYP4F22 is highly expressed at the site and timing of onset of keratinization during skin development. Sasaki K, et al. J Dermatol Sci, 2012 Feb. PMID 22209317.
  2. Systematic and quantitative assessment of the ubiquitin-modified proteome. Kim W, et al. Mol Cell, 2011 Oct 21. PMID 21906983.
  3. Finding homes for orphan cytochrome P450s: CYP4V2 and CYP4F22 in disease states. Kelly EJ, et al. Mol Interv, 2011 Apr. PMID 21540472.
  4. Pathogenesis of permeability barrier abnormalities in the ichthyoses: inherited disorders of lipid metabolism. Elias PM, et al. J Lipid Res, 2008 Apr. PMID 18245815.
  5. Mutations in a new cytochrome P450 gene in lamellar ichthyosis type 3. Lefèvre C, et al. Hum Mol Genet, 2006 Mar 1. PMID 16436457.

See all (10) citations in PubMed

See citations in PubMed for homologs of this gene provided by HomoloGene

GeneRIFs: Gene References Into Functions What's a GeneRIF?

  1. Letter: CYP4F22 is highly expressed at the site and timing of onset of keratinization during skin development.
    Title: CYP4F22 is highly expressed at the site and timing of onset of keratinization during skin development.
  2. REVIEW: genetic analyses have identified a wide spectrum of mutations in the CYP4V2gene from patients suffering from Bietti's crystalline corneoretinal dystrophy, and mutations in theCYP4F22 gene have been linked to lamellar ichthyosis
    Title: Finding homes for orphan cytochrome P450s: CYP4V2 and CYP4F22 in disease states.
Submit: New GeneRIF Correction

The following CYP4F22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CYP4F22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu18711
 XM_011527692 PREDICTED: Homo sapiens cytochrome P450, family 4, subfamily F, polypeptide 22 (CYP4F22), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $219.50-$307.30
$439.00
OHu18711
 XM_011527693 PREDICTED: Homo sapiens cytochrome P450, family 4, subfamily F, polypeptide 22 (CYP4F22), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $219.50-$307.30
$439.00
OHu18711
 NM_173483 Homo sapiens cytochrome P450, family 4, subfamily F, polypeptide 22 (CYP4F22), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $219.50-$307.30
$439.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu18711
Clone ID Related Accession (Same CDS sequence) NM_173483 , XM_011527692 , XM_011527693
Accession Version XM_011527692.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product cytochrome P450 4F22 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011295.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCTGCCCA TCACAGACCG CCTGCTGCAC CTCCTGGGGC TGGAGAAGAC GGCGTTCCGC 
ATATACGCGG TGTCCACCCT TCTCCTCTTC CTGCTCTTCT TCCTGTTCCG CCTGCTGCTG 
CGGTTCCTGA GGCTCTGCAG GAGCTTCTAC ATCACCTGCC GCCGGCTGCG CTGCTTCCCC 
CAGCCTCCCC GGCGCAACTG GCTGCTGGGC CACCTGGGCA TGTACCTTCC AAATGAGGCG 
GGCCTTCAAG ATGAGAAGAA GGTACTGGAC AACATGCACC ATGTACTCTT GGTATGGATG 
GGACCTGTCC TGCCGCTGTT GGTTCTGGTG CACCCTGATT ACATCAAACC CCTTTTGGGA 
GCCTCAGCTG CCATCGCCCC CAAGGATGAC CTCTTCTATG GCTTCCTAAA ACCTTGGCTA 
GGGGATGGGC TGCTGCTCAG CAAAGGTGAC AAGTGGAGCC GGCACCGTCG CCTGCTGACA 
CCCGCCTTCC ACTTTGACAT CCTGAAGCCT TACATGAAGA TCTTCAACCA GAGCGCTGAC 
ATTATGCATG CTAAATGGCG GCATCTGGCA GAGGGCTCAG CGGTCTCCCT TGATATGTTT 
GAGCATATCA GCCTCATGAC CCTGGACAGT CTTCAGAAAT GTGTCTTCAG CTACAACAGC 
AACTGCCAAG AGAAGATGAG TGATTATATC TCCGCTATCA TTGAACTGAG CGCTCTGTCT 
GTCCGGCGCC AGTATCGCTT GCACCACTAC CTCGACTTCA TTTACTACCG CTCGGCGGAT 
GGGCGGAGGT TCCGGCAGGC CTGTGACATG GTGCACCACT TCACCACTGA AGTCATCCAG 
GAACGGCGGC GGGCACTGCG TCAGCAGGGG GCCGAGGCCT GGCTTAAGGC CAAGCAGGGG 
AAGACCTTGG ACTTTATTGA TGTGCTGCTC CTGGCCAGGG ATGAAGATGG AAAGGAACTG 
TCAGACGAGG ATATCCGAGC CGAAGCAGAC ACCTTCATGT TTGAGGGTCA CGACACAACA 
TCCAGTGGGA TCTCTTGGAT GCTGTTCAAT TTGGCAAAGT ATCCGGAATA CCAGGAGAAA 
TGCCGAGAAG AGATTCAGGA AGTCATGAAA GGCCGGGAGC TGGAGGAGCT GGAGTGGGAC 
GATCTGACTC AGCTGCCCTT TACAACTATG TGCATTAAGG AGAGCCTGCG CCAGTACCCA 
CCTGTCACTC TTGTCTCTCG CCAATGCACG GAGGACATCA AGCTCCCAGA TGGGCGCATC 
ATCCCCAAAG GAATCATCTG CTTGGTCAGC ATCTATGGAA CCCACCACAA CCCCACAGTG 
TGGCCTGACT CCAAGGTGTA CAACCCCTAC CGCTTTGACC CGGACAACCC ACAGCAGCGC 
TCTCCACTGG CCTATGTGCC CTTCTCTGCA GGACCCAGGA ATTGCATCGG ACAGAGCTTC 
GCCATGGCCG AGTTGCGCGT GGTTGTGGCA CTAACACTGC TACGTTTCCG CCTGAGCGTG 
GACCGAACGC GCAAGGTGCG GCGGAAGCCG GAGCTCATAC TGCGCACGGA GAACGGGCTC 
TGGCTCAAGG TGGAGCCGCT GCCTCCGCGG GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011525994.1
CDS413..2008
Misc Feature(1)590..1984(+)
Translation

Target ORF information:

RefSeq Version XM_011527692
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cytochrome P450, family 4, subfamily F, polypeptide 22 (CYP4F22), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011527692

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCTGCCCA TCACAGACCG CCTGCTGCAC CTCCTGGGGC TGGAGAAGAC GGCGTTCCGC 
ATATACGCGG TGTCCACCCT TCTCCTCTTC CTGCTCTTCT TCCTGTTCCG CCTGCTGCTG 
CGGTTCCTGA GGCTCTGCAG GAGCTTCTAC ATCACCTGCC GCCGGCTGCG CTGCTTCCCC 
CAGCCTCCCC GGCGCAACTG GCTGCTGGGC CACCTGGGCA TGTACCTTCC AAATGAGGCG 
GGCCTTCAAG ATGAGAAGAA GGTACTGGAC AACATGCACC ATGTACTCTT GGTATGGATG 
GGACCTGTCC TGCCGCTGTT GGTTCTGGTG CACCCTGATT ACATCAAACC CCTTTTGGGA 
GCCTCAGCTG CCATCGCCCC CAAGGATGAC CTCTTCTATG GCTTCCTAAA ACCTTGGCTA 
GGGGATGGGC TGCTGCTCAG CAAAGGTGAC AAGTGGAGCC GGCACCGTCG CCTGCTGACA 
CCCGCCTTCC ACTTTGACAT CCTGAAGCCT TACATGAAGA TCTTCAACCA GAGCGCTGAC 
ATTATGCATG CTAAATGGCG GCATCTGGCA GAGGGCTCAG CGGTCTCCCT TGATATGTTT 
GAGCATATCA GCCTCATGAC CCTGGACAGT CTTCAGAAAT GTGTCTTCAG CTACAACAGC 
AACTGCCAAG AGAAGATGAG TGATTATATC TCCGCTATCA TTGAACTGAG CGCTCTGTCT 
GTCCGGCGCC AGTATCGCTT GCACCACTAC CTCGACTTCA TTTACTACCG CTCGGCGGAT 
GGGCGGAGGT TCCGGCAGGC CTGTGACATG GTGCACCACT TCACCACTGA AGTCATCCAG 
GAACGGCGGC GGGCACTGCG TCAGCAGGGG GCCGAGGCCT GGCTTAAGGC CAAGCAGGGG 
AAGACCTTGG ACTTTATTGA TGTGCTGCTC CTGGCCAGGG ATGAAGATGG AAAGGAACTG 
TCAGACGAGG ATATCCGAGC CGAAGCAGAC ACCTTCATGT TTGAGGGTCA CGACACAACA 
TCCAGTGGGA TCTCTTGGAT GCTGTTCAAT TTGGCAAAGT ATCCGGAATA CCAGGAGAAA 
TGCCGAGAAG AGATTCAGGA AGTCATGAAA GGCCGGGAGC TGGAGGAGCT GGAGTGGGAC 
GATCTGACTC AGCTGCCCTT TACAACTATG TGCATTAAGG AGAGCCTGCG CCAGTACCCA 
CCTGTCACTC TTGTCTCTCG CCAATGCACG GAGGACATCA AGCTCCCAGA TGGGCGCATC 
ATCCCCAAAG GAATCATCTG CTTGGTCAGC ATCTATGGAA CCCACCACAA CCCCACAGTG 
TGGCCTGACT CCAAGGTGTA CAACCCCTAC CGCTTTGACC CGGACAACCC ACAGCAGCGC 
TCTCCACTGG CCTATGTGCC CTTCTCTGCA GGACCCAGGA ATTGCATCGG ACAGAGCTTC 
GCCATGGCCG AGTTGCGCGT GGTTGTGGCA CTAACACTGC TACGTTTCCG CCTGAGCGTG 
GACCGAACGC GCAAGGTGCG GCGGAAGCCG GAGCTCATAC TGCGCACGGA GAACGGGCTC 
TGGCTCAAGG TGGAGCCGCT GCCTCCGCGG GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18711
Clone ID Related Accession (Same CDS sequence) NM_173483 , XM_011527692 , XM_011527693
Accession Version XM_011527693.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product cytochrome P450 4F22 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011295.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCTGCCCA TCACAGACCG CCTGCTGCAC CTCCTGGGGC TGGAGAAGAC GGCGTTCCGC 
ATATACGCGG TGTCCACCCT TCTCCTCTTC CTGCTCTTCT TCCTGTTCCG CCTGCTGCTG 
CGGTTCCTGA GGCTCTGCAG GAGCTTCTAC ATCACCTGCC GCCGGCTGCG CTGCTTCCCC 
CAGCCTCCCC GGCGCAACTG GCTGCTGGGC CACCTGGGCA TGTACCTTCC AAATGAGGCG 
GGCCTTCAAG ATGAGAAGAA GGTACTGGAC AACATGCACC ATGTACTCTT GGTATGGATG 
GGACCTGTCC TGCCGCTGTT GGTTCTGGTG CACCCTGATT ACATCAAACC CCTTTTGGGA 
GCCTCAGCTG CCATCGCCCC CAAGGATGAC CTCTTCTATG GCTTCCTAAA ACCTTGGCTA 
GGGGATGGGC TGCTGCTCAG CAAAGGTGAC AAGTGGAGCC GGCACCGTCG CCTGCTGACA 
CCCGCCTTCC ACTTTGACAT CCTGAAGCCT TACATGAAGA TCTTCAACCA GAGCGCTGAC 
ATTATGCATG CTAAATGGCG GCATCTGGCA GAGGGCTCAG CGGTCTCCCT TGATATGTTT 
GAGCATATCA GCCTCATGAC CCTGGACAGT CTTCAGAAAT GTGTCTTCAG CTACAACAGC 
AACTGCCAAG AGAAGATGAG TGATTATATC TCCGCTATCA TTGAACTGAG CGCTCTGTCT 
GTCCGGCGCC AGTATCGCTT GCACCACTAC CTCGACTTCA TTTACTACCG CTCGGCGGAT 
GGGCGGAGGT TCCGGCAGGC CTGTGACATG GTGCACCACT TCACCACTGA AGTCATCCAG 
GAACGGCGGC GGGCACTGCG TCAGCAGGGG GCCGAGGCCT GGCTTAAGGC CAAGCAGGGG 
AAGACCTTGG ACTTTATTGA TGTGCTGCTC CTGGCCAGGG ATGAAGATGG AAAGGAACTG 
TCAGACGAGG ATATCCGAGC CGAAGCAGAC ACCTTCATGT TTGAGGGTCA CGACACAACA 
TCCAGTGGGA TCTCTTGGAT GCTGTTCAAT TTGGCAAAGT ATCCGGAATA CCAGGAGAAA 
TGCCGAGAAG AGATTCAGGA AGTCATGAAA GGCCGGGAGC TGGAGGAGCT GGAGTGGGAC 
GATCTGACTC AGCTGCCCTT TACAACTATG TGCATTAAGG AGAGCCTGCG CCAGTACCCA 
CCTGTCACTC TTGTCTCTCG CCAATGCACG GAGGACATCA AGCTCCCAGA TGGGCGCATC 
ATCCCCAAAG GAATCATCTG CTTGGTCAGC ATCTATGGAA CCCACCACAA CCCCACAGTG 
TGGCCTGACT CCAAGGTGTA CAACCCCTAC CGCTTTGACC CGGACAACCC ACAGCAGCGC 
TCTCCACTGG CCTATGTGCC CTTCTCTGCA GGACCCAGGA ATTGCATCGG ACAGAGCTTC 
GCCATGGCCG AGTTGCGCGT GGTTGTGGCA CTAACACTGC TACGTTTCCG CCTGAGCGTG 
GACCGAACGC GCAAGGTGCG GCGGAAGCCG GAGCTCATAC TGCGCACGGA GAACGGGCTC 
TGGCTCAAGG TGGAGCCGCT GCCTCCGCGG GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011525995.1
CDS203..1798
Misc Feature(1)380..1774(+)
Translation

Target ORF information:

RefSeq Version XM_011527693
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cytochrome P450, family 4, subfamily F, polypeptide 22 (CYP4F22), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011527693

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCTGCCCA TCACAGACCG CCTGCTGCAC CTCCTGGGGC TGGAGAAGAC GGCGTTCCGC 
ATATACGCGG TGTCCACCCT TCTCCTCTTC CTGCTCTTCT TCCTGTTCCG CCTGCTGCTG 
CGGTTCCTGA GGCTCTGCAG GAGCTTCTAC ATCACCTGCC GCCGGCTGCG CTGCTTCCCC 
CAGCCTCCCC GGCGCAACTG GCTGCTGGGC CACCTGGGCA TGTACCTTCC AAATGAGGCG 
GGCCTTCAAG ATGAGAAGAA GGTACTGGAC AACATGCACC ATGTACTCTT GGTATGGATG 
GGACCTGTCC TGCCGCTGTT GGTTCTGGTG CACCCTGATT ACATCAAACC CCTTTTGGGA 
GCCTCAGCTG CCATCGCCCC CAAGGATGAC CTCTTCTATG GCTTCCTAAA ACCTTGGCTA 
GGGGATGGGC TGCTGCTCAG CAAAGGTGAC AAGTGGAGCC GGCACCGTCG CCTGCTGACA 
CCCGCCTTCC ACTTTGACAT CCTGAAGCCT TACATGAAGA TCTTCAACCA GAGCGCTGAC 
ATTATGCATG CTAAATGGCG GCATCTGGCA GAGGGCTCAG CGGTCTCCCT TGATATGTTT 
GAGCATATCA GCCTCATGAC CCTGGACAGT CTTCAGAAAT GTGTCTTCAG CTACAACAGC 
AACTGCCAAG AGAAGATGAG TGATTATATC TCCGCTATCA TTGAACTGAG CGCTCTGTCT 
GTCCGGCGCC AGTATCGCTT GCACCACTAC CTCGACTTCA TTTACTACCG CTCGGCGGAT 
GGGCGGAGGT TCCGGCAGGC CTGTGACATG GTGCACCACT TCACCACTGA AGTCATCCAG 
GAACGGCGGC GGGCACTGCG TCAGCAGGGG GCCGAGGCCT GGCTTAAGGC CAAGCAGGGG 
AAGACCTTGG ACTTTATTGA TGTGCTGCTC CTGGCCAGGG ATGAAGATGG AAAGGAACTG 
TCAGACGAGG ATATCCGAGC CGAAGCAGAC ACCTTCATGT TTGAGGGTCA CGACACAACA 
TCCAGTGGGA TCTCTTGGAT GCTGTTCAAT TTGGCAAAGT ATCCGGAATA CCAGGAGAAA 
TGCCGAGAAG AGATTCAGGA AGTCATGAAA GGCCGGGAGC TGGAGGAGCT GGAGTGGGAC 
GATCTGACTC AGCTGCCCTT TACAACTATG TGCATTAAGG AGAGCCTGCG CCAGTACCCA 
CCTGTCACTC TTGTCTCTCG CCAATGCACG GAGGACATCA AGCTCCCAGA TGGGCGCATC 
ATCCCCAAAG GAATCATCTG CTTGGTCAGC ATCTATGGAA CCCACCACAA CCCCACAGTG 
TGGCCTGACT CCAAGGTGTA CAACCCCTAC CGCTTTGACC CGGACAACCC ACAGCAGCGC 
TCTCCACTGG CCTATGTGCC CTTCTCTGCA GGACCCAGGA ATTGCATCGG ACAGAGCTTC 
GCCATGGCCG AGTTGCGCGT GGTTGTGGCA CTAACACTGC TACGTTTCCG CCTGAGCGTG 
GACCGAACGC GCAAGGTGCG GCGGAAGCCG GAGCTCATAC TGCGCACGGA GAACGGGCTC 
TGGCTCAAGG TGGAGCCGCT GCCTCCGCGG GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18711
Clone ID Related Accession (Same CDS sequence) NM_173483 , XM_011527692 , XM_011527693
Accession Version NM_173483.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cytochrome P450 4F22
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA874258.1, BC069351.1, AC011492.8 and AI341068.1. This sequence is a reference standard in the RefSeqGene project. On Oct 11, 2007 this sequence version replaced gi:142377118. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK096820.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCTGCCCA TCACAGACCG CCTGCTGCAC CTCCTGGGGC TGGAGAAGAC GGCGTTCCGC 
ATATACGCGG TGTCCACCCT TCTCCTCTTC CTGCTCTTCT TCCTGTTCCG CCTGCTGCTG 
CGGTTCCTGA GGCTCTGCAG GAGCTTCTAC ATCACCTGCC GCCGGCTGCG CTGCTTCCCC 
CAGCCTCCCC GGCGCAACTG GCTGCTGGGC CACCTGGGCA TGTACCTTCC AAATGAGGCG 
GGCCTTCAAG ATGAGAAGAA GGTACTGGAC AACATGCACC ATGTACTCTT GGTATGGATG 
GGACCTGTCC TGCCGCTGTT GGTTCTGGTG CACCCTGATT ACATCAAACC CCTTTTGGGA 
GCCTCAGCTG CCATCGCCCC CAAGGATGAC CTCTTCTATG GCTTCCTAAA ACCTTGGCTA 
GGGGATGGGC TGCTGCTCAG CAAAGGTGAC AAGTGGAGCC GGCACCGTCG CCTGCTGACA 
CCCGCCTTCC ACTTTGACAT CCTGAAGCCT TACATGAAGA TCTTCAACCA GAGCGCTGAC 
ATTATGCATG CTAAATGGCG GCATCTGGCA GAGGGCTCAG CGGTCTCCCT TGATATGTTT 
GAGCATATCA GCCTCATGAC CCTGGACAGT CTTCAGAAAT GTGTCTTCAG CTACAACAGC 
AACTGCCAAG AGAAGATGAG TGATTATATC TCCGCTATCA TTGAACTGAG CGCTCTGTCT 
GTCCGGCGCC AGTATCGCTT GCACCACTAC CTCGACTTCA TTTACTACCG CTCGGCGGAT 
GGGCGGAGGT TCCGGCAGGC CTGTGACATG GTGCACCACT TCACCACTGA AGTCATCCAG 
GAACGGCGGC GGGCACTGCG TCAGCAGGGG GCCGAGGCCT GGCTTAAGGC CAAGCAGGGG 
AAGACCTTGG ACTTTATTGA TGTGCTGCTC CTGGCCAGGG ATGAAGATGG AAAGGAACTG 
TCAGACGAGG ATATCCGAGC CGAAGCAGAC ACCTTCATGT TTGAGGGTCA CGACACAACA 
TCCAGTGGGA TCTCTTGGAT GCTGTTCAAT TTGGCAAAGT ATCCGGAATA CCAGGAGAAA 
TGCCGAGAAG AGATTCAGGA AGTCATGAAA GGCCGGGAGC TGGAGGAGCT GGAGTGGGAC 
GATCTGACTC AGCTGCCCTT TACAACTATG TGCATTAAGG AGAGCCTGCG CCAGTACCCA 
CCTGTCACTC TTGTCTCTCG CCAATGCACG GAGGACATCA AGCTCCCAGA TGGGCGCATC 
ATCCCCAAAG GAATCATCTG CTTGGTCAGC ATCTATGGAA CCCACCACAA CCCCACAGTG 
TGGCCTGACT CCAAGGTGTA CAACCCCTAC CGCTTTGACC CGGACAACCC ACAGCAGCGC 
TCTCCACTGG CCTATGTGCC CTTCTCTGCA GGACCCAGGA ATTGCATCGG ACAGAGCTTC 
GCCATGGCCG AGTTGCGCGT GGTTGTGGCA CTAACACTGC TACGTTTCCG CCTGAGCGTG 
GACCGAACGC GCAAGGTGCG GCGGAAGCCG GAGCTCATAC TGCGCACGGA GAACGGGCTC 
TGGCTCAAGG TGGAGCCGCT GCCTCCGCGG GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_775754.2
CDS168..1763
Misc Feature(1)93..95(+)
Misc Feature(2)345..1739(+)
Exon (1)1..59
Gene:CYP4F22
Gene Synonym:
Exon (2)60..166
Gene:CYP4F22
Gene Synonym:
Exon (3)167..389
Gene:CYP4F22
Gene Synonym:
Exon (4)390..534
Gene:CYP4F22
Gene Synonym:
Exon (5)535..588
Gene:CYP4F22
Gene Synonym:
Exon (6)589..716
Gene:CYP4F22
Gene Synonym:
Exon (7)717..838
Gene:CYP4F22
Gene Synonym:
Exon (8)839..1106
Gene:CYP4F22
Gene Synonym:
Exon (9)1107..1173
Gene:CYP4F22
Gene Synonym:
Exon (10)1174..1303
Gene:CYP4F22
Gene Synonym:
Exon (11)1304..1437
Gene:CYP4F22
Gene Synonym:
Exon (12)1438..1502
Gene:CYP4F22
Gene Synonym:
Exon (13)1503..1585
Gene:CYP4F22
Gene Synonym:
Exon (14)1586..2609
Gene:CYP4F22
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_173483
Organism Homo sapiens (human)
Definition Homo sapiens cytochrome P450, family 4, subfamily F, polypeptide 22 (CYP4F22), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_173483

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCTGCCCA TCACAGACCG CCTGCTGCAC CTCCTGGGGC TGGAGAAGAC GGCGTTCCGC 
ATATACGCGG TGTCCACCCT TCTCCTCTTC CTGCTCTTCT TCCTGTTCCG CCTGCTGCTG 
CGGTTCCTGA GGCTCTGCAG GAGCTTCTAC ATCACCTGCC GCCGGCTGCG CTGCTTCCCC 
CAGCCTCCCC GGCGCAACTG GCTGCTGGGC CACCTGGGCA TGTACCTTCC AAATGAGGCG 
GGCCTTCAAG ATGAGAAGAA GGTACTGGAC AACATGCACC ATGTACTCTT GGTATGGATG 
GGACCTGTCC TGCCGCTGTT GGTTCTGGTG CACCCTGATT ACATCAAACC CCTTTTGGGA 
GCCTCAGCTG CCATCGCCCC CAAGGATGAC CTCTTCTATG GCTTCCTAAA ACCTTGGCTA 
GGGGATGGGC TGCTGCTCAG CAAAGGTGAC AAGTGGAGCC GGCACCGTCG CCTGCTGACA 
CCCGCCTTCC ACTTTGACAT CCTGAAGCCT TACATGAAGA TCTTCAACCA GAGCGCTGAC 
ATTATGCATG CTAAATGGCG GCATCTGGCA GAGGGCTCAG CGGTCTCCCT TGATATGTTT 
GAGCATATCA GCCTCATGAC CCTGGACAGT CTTCAGAAAT GTGTCTTCAG CTACAACAGC 
AACTGCCAAG AGAAGATGAG TGATTATATC TCCGCTATCA TTGAACTGAG CGCTCTGTCT 
GTCCGGCGCC AGTATCGCTT GCACCACTAC CTCGACTTCA TTTACTACCG CTCGGCGGAT 
GGGCGGAGGT TCCGGCAGGC CTGTGACATG GTGCACCACT TCACCACTGA AGTCATCCAG 
GAACGGCGGC GGGCACTGCG TCAGCAGGGG GCCGAGGCCT GGCTTAAGGC CAAGCAGGGG 
AAGACCTTGG ACTTTATTGA TGTGCTGCTC CTGGCCAGGG ATGAAGATGG AAAGGAACTG 
TCAGACGAGG ATATCCGAGC CGAAGCAGAC ACCTTCATGT TTGAGGGTCA CGACACAACA 
TCCAGTGGGA TCTCTTGGAT GCTGTTCAAT TTGGCAAAGT ATCCGGAATA CCAGGAGAAA 
TGCCGAGAAG AGATTCAGGA AGTCATGAAA GGCCGGGAGC TGGAGGAGCT GGAGTGGGAC 
GATCTGACTC AGCTGCCCTT TACAACTATG TGCATTAAGG AGAGCCTGCG CCAGTACCCA 
CCTGTCACTC TTGTCTCTCG CCAATGCACG GAGGACATCA AGCTCCCAGA TGGGCGCATC 
ATCCCCAAAG GAATCATCTG CTTGGTCAGC ATCTATGGAA CCCACCACAA CCCCACAGTG 
TGGCCTGACT CCAAGGTGTA CAACCCCTAC CGCTTTGACC CGGACAACCC ACAGCAGCGC 
TCTCCACTGG CCTATGTGCC CTTCTCTGCA GGACCCAGGA ATTGCATCGG ACAGAGCTTC 
GCCATGGCCG AGTTGCGCGT GGTTGTGGCA CTAACACTGC TACGTTTCCG CCTGAGCGTG 
GACCGAACGC GCAAGGTGCG GCGGAAGCCG GAGCTCATAC TGCGCACGGA GAACGGGCTC 
TGGCTCAAGG TGGAGCCGCT GCCTCCGCGG GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

 
*
*
*
*