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COL1A1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol COL1A1
Entrez Gene ID 1277
Full Name collagen type I alpha 1 chain
Synonyms EDSC,OI1,OI2,OI3,OI4
General protein information
Preferred Names

collagen type I alpha 1 chain

Names

collagen alpha-1(I) chain
alpha-1 type I collagen
alpha1(I) procollagen
collagen alpha 1 chain type I
collagen alpha-1(I) chain preproprotein
collagen of skin, tendon and bone, alpha-1 chain
collagen, type I, alpha 1
pro-alpha-1 collagen type 1
type I proalpha 1
type I procollagen alpha 1 chain

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

17

17q21.33

Summary This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008].
Disorder MIM:

120150

Disorder Html: Osteogenesis imperfecta, type I, 166200 (3); OI type II, 166210 (3);

mRNA and Protein(s)

mRNA Protein Name
XM_005257058.3 XP_005257115.2 collagen alpha-1(I) chain isoform X2
XM_005257059.4 XP_005257116.2 collagen alpha-1(I) chain isoform X3
NM_000088.3 NP_000079.2 collagen alpha-1(I) chain preproprotein
XM_011524341.1 XP_011522643.1 collagen alpha-1(I) chain isoform X1



Bos taurus (cattle) COL1A1 NP_001029211.1
Mus musculus (house mouse) Col1a1 NP_031768.2
Danio rerio (zebrafish) col1a1a NP_954684.1
Pan troglodytes (chimpanzee) COL1A1 XP_001169409.1
Canis lupus familiaris (dog) COL1A1 NP_001003090.1
Rattus norvegicus (Norway rat) Col1a1 NP_445756.1
Macaca mulatta (rhesus monkey) COL1A1 XP_001096194.2
Homo sapiens (human) COL1A1 NP_000079.2
Xenopus tropicalis (tropical clawed frog) LOC496414 NP_001011005.1


Related articles in PubMed

Effects of GABA on the expression of type I collagen gene in normal human dermal fibroblasts.
Uehara E, Hokazono H, Sasaki T, Yoshioka H, Matsuo N
Bioscience, biotechnology, and biochemistry81(2)376-379(2017 Feb)

Collagen analysis of the ascending aortic dilatation associated with bicuspid aortic valve disease compared with tricuspid aortic valve.
Navarrete Santos A, Yan J, Lochmann P, Pfeil H, Petersen M, Simm A, Treede H, Sievers HH, Mohamed SA
Archives of physiology and biochemistry122(5)289-294(2016 Dec)

Identification of COL1A1 and COL1A2 as candidate prognostic factors in gastric cancer.
Li J, Ding Y, Li A
World journal of surgical oncology14(1)297(2016 Nov)

Clinical characteristics and the identification of novel mutations of COL1A1 and COL1A2 in 61 Chinese patients with osteogenesis imperfecta.
Zhang H, Yue H, Wang C, Hu W, Gu J, He J, Fu W, Hu Y, Li M, Zhang Z
Molecular medicine reports14(5)4918-4926(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Femoral and tibial cartilage show a different behaviour concerning expression values for the genes Col1A1, Col2A1 and Agg.
Title: Regional differences of tibial and femoral cartilage in the chondrocyte gene expression, immunhistochemistry and composite in different stages of osteoarthritis.

overexpressed miR185 could inhibit hypertrophic scarring fibroblast growth, and the underlying mechanism was mediated, at least partly, through the suppression of TGFbeta1 and Col1 expression.
Title: MicroRNA‑185 regulates transforming growth factor‑β1 and collagen‑1 in hypertrophic scar fibroblasts.

GABA improves the skin elasticity by regulating type I collagen expression
Title: Effects of GABA on the expression of type I collagen gene in normal human dermal fibroblasts.

High collgen expression is associated with ascending aortic dilatation.
Title: Collagen analysis of the ascending aortic dilatation associated with bicuspid aortic valve disease compared with tricuspid aortic valve.

COL11A1 as a highly specific biomarker of activated cancer-associated fibroblasts in epithelial cancers.
Title: A COL11A1-correlated pan-cancer gene signature of activated fibroblasts for the prioritization of therapeutic targets.

The following COL1A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL1A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu50115 XM_005257059.4
Latest version!
Homo sapiens collagen type I alpha 1 (COL1A1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu25462 NM_000088.3
Latest version!
Homo sapiens collagen type I alpha 1 chain (COL1A1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $69.30
$99.00
OHu50114 XM_005257058.3
Latest version!
Homo sapiens collagen type I alpha 1 (COL1A1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu56962 XM_011524341.1
Latest version!
Homo sapiens collagen type I alpha 1 (COL1A1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $727.30
$1039.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu50115
Clone ID Related Accession (Same CDS sequence) XM_005257059.4
Accession Version XM_005257059.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3477bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product collagen alpha-1(I) chain isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005257059.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGTTCAGCT TTGTGGACCT CCGGCTCCTG CTCCTCTTAG CGGCCACCGC CCTCCTGACG 
CACGGCCAAG AGGAAGGCCA AGTCGAGGGC CAAGACGAAG ACATCCCACC AATCACCTGC
GTACAGAACG GCCTCAGGTA CCATGACCGA GACGTGTGGA AACCCGAGCC CTGCCGGATC
TGCGTCTGCG ACAACGGCAA GGTGTTGTGC GATGACGTGA TCTGTGACGA GACCAAGAAC
TGCCCCGGCG CCGAAGTCCC CGAGGGCGAG TGCTGTCCCG TCTGCCCCGA CGGCTCAGAG
TCACCCACCG ACCAAGAAAC CACCGGCGTC GAGGGACCCA AGGGAGACAC TGGCCCCCGA
GGCCCAAGGG GACCCGCAGG CCCCCCTGGC CGAGATGGCA TCCCTGGACA GCCTGGACTT
CCCGGACCCC CCGGACCCCC CGGACCTCCC GGACCCCCTG GCCTCGGAGG AAACTTTGCT
CCCCAGCTGT CTTATGGCTA TGATGAGAAA TCAACCGGAG GAATTTCCGT GCCTGGCCCC
ATGGGTCCCT CTGGTCCTCG TGGTCTCCCT GGCCCCCCTG GTGCACCTGG TCCCCAAGGC
TTCCAAGGTC CCCCTGGTGA GCCTGGCGAG CCTGGAGCTT CAGGTCCCAT GGGTCCCCGA
GGTCCCCCAG GTCCCCCTGG AAAGAATGGA GATGATGGGG AAGCTGGAAA ACCTGGTCGT
CCTGGTGAGC GTGGGCCTCC TGGGCCTCAG GGTGCTCGAG GATTGCCCGG AACAGCTGGC
CTCCCTGGAA TGAAGGGACA CAGAGGTTTC AGTGGTTTGG ATGGTGCCAA GGGAGATGCT
GGTCCTGCTG GTCCTAAGGG TGAGCCTGGC AGCCCTGGTG AAAATGGAGC TCCTGGTCAG
ATGGGCCCCC GTGGCCTGCC TGGTGAGAGA GGTCGCCCTG GAGCCCCTGG CCCTGCTGGT
CCCGCTGGCG AGAGAGGTGA ACAAGGCCCT GCTGGCTCCC CCGGATTCCA GGGTCTCCCT
GGTCCTGCTG GTCCTCCAGG TGAAGCAGGC AAACCTGGTG AACAGGGTGT TCCTGGAGAC
CTTGGCGCCC CTGGCCCCTC TGGAGCAAGA GGCGAGAGAG GTTTCCCTGG CGAGCGTGGT
GTGCAAGGTC CCCCTGGTCC TGCTGGTCCC CGAGGGGCCA ACGGTGCTCC CGGCAACGAT
GGTGCTAAGG GTGATGCTGG TGCCCCTGGA GCTCCCGGTA GCCAGGGCGC CCCTGGCCTT
CAGGGAATGC CTGGTGAACG TGGTGCAGCT GGTCTTCCAG GGCCTAAGGG TGACAGAGGT
GATGCTGGTC CCAAAGGTGC TGATGGCTCT CCTGGCAAAG ATGGCGTCCG TGGTCTGACT
GGCCCCATTG GTCCTCCTGG CCCTGCTGGT GCCCCTGGTG ACAAGGGTGA AAGTGGTCCC
AGCGGCCCTG CTGGTCCCAC TGGAGCTCGT GGTGCCCCCG GAGACCGTGG TGAGCCTGGT
CCCCCCGGCC CTGCTGGCTT TGCTGGCCCC CCTGGTGCTG ACGGCCAACC TGGTGCTAAA
GGCGAACCTG GTGATGCTGG TGCTAAAGGC GATGCTGGTC CCCCTGGCCC TGCCGGACCC
GCTGGACCCC CTGGCCCCAT TGGTAATGTT GGTGCTCCTG GAGCCAAAGG TGCTCGCGGC
AGCGCTGGTC CCCCTGGTGC TACTGGTTTC CCTGGTGCTG CTGGCCGAGT CGGTCCTCCT
GGCCCCTCTG GAAATGCTGG ACCCCCTGGC CCTCCTGGTC CTGCTGGCAA AGAAGGCGGC
AAAGGTCCCC GTGGTGAGAC TGGCCCTGCT GGACGTCCTG GTGAAGTTGG TCCCCCTGGT
CCCCCTGGCC CTGCTGGCGA GAAAGGATCC CCTGGTGCTG ATGGTCCTGC TGGTGCTCCT
GGTACTCCCG GGCCTCAAGG TATTGCTGGA CAGCGTGGTG TGGTCGGCCT GCCTGGTCAG
AGAGGAGAGA GAGGCTTCCC TGGTCTTCCT GGCCCCTCTG GTGAACCTGG CAAACAAGGT
CCCTCTGGAG CAAGTGGTGA ACGTGGTCCC CCTGGTCCCA TGGGCCCCCC TGGATTGGCT
GGACCCCCTG GTGAATCTGG ACGTGAGGGG GCTCCTGGTG CCGAAGGTTC CCCTGGACGA
GACGGTTCTC CTGGCGCCAA GGGTGACCGT GGTGAGACCG GCCCCGCTGG ACCCCCTGGT
GCTCCTGGTG CTCCTGGTGC CCCTGGCCCC GTTGGCCCTG CTGGCAAGAG TGGTGATCGT
GGTGAGACTG GTCCTGCTGG TCCCGCCGGT CCTGTCGGCC CTGTTGGCGC CCGTGGCCCC
GCCGGACCCC AAGGCCCCCG TGGTGACAAG GGTGAGACAG GCGAACAGGG CGACAGAGGC
ATAAAGGGTC ACCGTGGCTT CTCTGGCCTC CAGGGTCCCC CTGGCCCTCC TGGCTCTCCT
GGTGAACAAG GTCCCTCTGG AGCCTCTGGT CCTGCTGGTC CCCGAGGTCC CCCTGGCTCT
GCTGGTGCTC CTGGCAAAGA TGGACTCAAC GGTCTCCCTG GCCCCATTGG GCCCCCTGGT
CCTCGCGGTC GCACTGGTGA TGCTGGTCCT GTTGGTCCCC CCGGCCCTCC TGGACCTCCT
GGTCCCCCTG GTCCTCCCAG CGCTGGTTTC GACTTCAGCT TCCTGCCCCA GCCACCTCAA
GAGAAGGCTC ACGATGGTGG CCGCTACTAC CGGGCTGATG ATGCCAATGT GGTTCGTGAC
CGTGACCTCG AGGTGGACAC CACCCTCAAG AGCCTGAGCC AGCAGATCGA GAACATCCGG
AGCCCAGAGG GCAGCCGCAA GAACCCCGCC CGCACCTGCC GTGACCTCAA GATGTGCCAC
TCTGACTGGA AGAGTGGAGA GTACTGGATT GACCCCAACC AAGGCTGCAA CCTGGATGCC
ATCAAAGTCT TCTGCAACAT GGAGACTGGT GAGACCTGCG TGTACCCCAC TCAGCCCAGT
GTGGCCCAGA AGAACTGGTA CATCAGCAAG AACCCCAAGG ACAAGAGGCA TGTCTGGTTC
GGCGAGAGCA TGACCGATGG ATTCCAGTTC GAGTATGGCG GCCAGGGCTC CGACCCTGCC
GATGTGGCCA TCCAGCTGAC CTTCCTGCGC CTGATGTCCA CCGAGGCCTC CCAGAACATC
ACCTACCACT GCAAGAACAG CGTGGCCTAC ATGGACCAGC AGACTGGCAA CCTCAAGAAG
GCCCTGCTCC TCCAGGGCTC CAACGAGATC GAGATCCGCG CCGAGGGCAA CAGCCGCTTC
ACCTACAGCG TCACTGTCGA TGGCTGCACG AGTCACACCG GAGCCTGGGG CAAGACAGTG
ATTGAATACA AAACCACCAA GACCTCCCGC CTGCCCATCA TCGATGTGGC CCCCTTGGAC
GTTGGTGCCC CAGACCAGGA ATTCGGCTTC GACGTTGGCC CTGTCTGCTT CCTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005257116.2
CDS136..3612
Misc Feature(1)253..420
Misc Feature(2)841..1020
Misc Feature(3)1219..1392
Misc Feature(4)1318..1494
Misc Feature(5)2905..3606
Translation

Target ORF information:

RefSeq Version XM_005257059.4
Organism Homo sapiens(Human)
Definition Homo sapiens collagen type I alpha 1 (COL1A1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257059.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGTTCAGCT TTGTGGACCT CCGGCTCCTG CTCCTCTTAG CGGCCACCGC CCTCCTGACG 
CACGGCCAAG AGGAAGGCCA AGTCGAGGGC CAAGACGAAG ACATCCCACC AATCACCTGC
GTACAGAACG GCCTCAGGTA CCATGACCGA GACGTGTGGA AACCCGAGCC CTGCCGGATC
TGCGTCTGCG ACAACGGCAA GGTGTTGTGC GATGACGTGA TCTGTGACGA GACCAAGAAC
TGCCCCGGCG CCGAAGTCCC CGAGGGCGAG TGCTGTCCCG TCTGCCCCGA CGGCTCAGAG
TCACCCACCG ACCAAGAAAC CACCGGCGTC GAGGGACCCA AGGGAGACAC TGGCCCCCGA
GGCCCAAGGG GACCCGCAGG CCCCCCTGGC CGAGATGGCA TCCCTGGACA GCCTGGACTT
CCCGGACCCC CCGGACCCCC CGGACCTCCC GGACCCCCTG GCCTCGGAGG AAACTTTGCT
CCCCAGCTGT CTTATGGCTA TGATGAGAAA TCAACCGGAG GAATTTCCGT GCCTGGCCCC
ATGGGTCCCT CTGGTCCTCG TGGTCTCCCT GGCCCCCCTG GTGCACCTGG TCCCCAAGGC
TTCCAAGGTC CCCCTGGTGA GCCTGGCGAG CCTGGAGCTT CAGGTCCCAT GGGTCCCCGA
GGTCCCCCAG GTCCCCCTGG AAAGAATGGA GATGATGGGG AAGCTGGAAA ACCTGGTCGT
CCTGGTGAGC GTGGGCCTCC TGGGCCTCAG GGTGCTCGAG GATTGCCCGG AACAGCTGGC
CTCCCTGGAA TGAAGGGACA CAGAGGTTTC AGTGGTTTGG ATGGTGCCAA GGGAGATGCT
GGTCCTGCTG GTCCTAAGGG TGAGCCTGGC AGCCCTGGTG AAAATGGAGC TCCTGGTCAG
ATGGGCCCCC GTGGCCTGCC TGGTGAGAGA GGTCGCCCTG GAGCCCCTGG CCCTGCTGGT
CCCGCTGGCG AGAGAGGTGA ACAAGGCCCT GCTGGCTCCC CCGGATTCCA GGGTCTCCCT
GGTCCTGCTG GTCCTCCAGG TGAAGCAGGC AAACCTGGTG AACAGGGTGT TCCTGGAGAC
CTTGGCGCCC CTGGCCCCTC TGGAGCAAGA GGCGAGAGAG GTTTCCCTGG CGAGCGTGGT
GTGCAAGGTC CCCCTGGTCC TGCTGGTCCC CGAGGGGCCA ACGGTGCTCC CGGCAACGAT
GGTGCTAAGG GTGATGCTGG TGCCCCTGGA GCTCCCGGTA GCCAGGGCGC CCCTGGCCTT
CAGGGAATGC CTGGTGAACG TGGTGCAGCT GGTCTTCCAG GGCCTAAGGG TGACAGAGGT
GATGCTGGTC CCAAAGGTGC TGATGGCTCT CCTGGCAAAG ATGGCGTCCG TGGTCTGACT
GGCCCCATTG GTCCTCCTGG CCCTGCTGGT GCCCCTGGTG ACAAGGGTGA AAGTGGTCCC
AGCGGCCCTG CTGGTCCCAC TGGAGCTCGT GGTGCCCCCG GAGACCGTGG TGAGCCTGGT
CCCCCCGGCC CTGCTGGCTT TGCTGGCCCC CCTGGTGCTG ACGGCCAACC TGGTGCTAAA
GGCGAACCTG GTGATGCTGG TGCTAAAGGC GATGCTGGTC CCCCTGGCCC TGCCGGACCC
GCTGGACCCC CTGGCCCCAT TGGTAATGTT GGTGCTCCTG GAGCCAAAGG TGCTCGCGGC
AGCGCTGGTC CCCCTGGTGC TACTGGTTTC CCTGGTGCTG CTGGCCGAGT CGGTCCTCCT
GGCCCCTCTG GAAATGCTGG ACCCCCTGGC CCTCCTGGTC CTGCTGGCAA AGAAGGCGGC
AAAGGTCCCC GTGGTGAGAC TGGCCCTGCT GGACGTCCTG GTGAAGTTGG TCCCCCTGGT
CCCCCTGGCC CTGCTGGCGA GAAAGGATCC CCTGGTGCTG ATGGTCCTGC TGGTGCTCCT
GGTACTCCCG GGCCTCAAGG TATTGCTGGA CAGCGTGGTG TGGTCGGCCT GCCTGGTCAG
AGAGGAGAGA GAGGCTTCCC TGGTCTTCCT GGCCCCTCTG GTGAACCTGG CAAACAAGGT
CCCTCTGGAG CAAGTGGTGA ACGTGGTCCC CCTGGTCCCA TGGGCCCCCC TGGATTGGCT
GGACCCCCTG GTGAATCTGG ACGTGAGGGG GCTCCTGGTG CCGAAGGTTC CCCTGGACGA
GACGGTTCTC CTGGCGCCAA GGGTGACCGT GGTGAGACCG GCCCCGCTGG ACCCCCTGGT
GCTCCTGGTG CTCCTGGTGC CCCTGGCCCC GTTGGCCCTG CTGGCAAGAG TGGTGATCGT
GGTGAGACTG GTCCTGCTGG TCCCGCCGGT CCTGTCGGCC CTGTTGGCGC CCGTGGCCCC
GCCGGACCCC AAGGCCCCCG TGGTGACAAG GGTGAGACAG GCGAACAGGG CGACAGAGGC
ATAAAGGGTC ACCGTGGCTT CTCTGGCCTC CAGGGTCCCC CTGGCCCTCC TGGCTCTCCT
GGTGAACAAG GTCCCTCTGG AGCCTCTGGT CCTGCTGGTC CCCGAGGTCC CCCTGGCTCT
GCTGGTGCTC CTGGCAAAGA TGGACTCAAC GGTCTCCCTG GCCCCATTGG GCCCCCTGGT
CCTCGCGGTC GCACTGGTGA TGCTGGTCCT GTTGGTCCCC CCGGCCCTCC TGGACCTCCT
GGTCCCCCTG GTCCTCCCAG CGCTGGTTTC GACTTCAGCT TCCTGCCCCA GCCACCTCAA
GAGAAGGCTC ACGATGGTGG CCGCTACTAC CGGGCTGATG ATGCCAATGT GGTTCGTGAC
CGTGACCTCG AGGTGGACAC CACCCTCAAG AGCCTGAGCC AGCAGATCGA GAACATCCGG
AGCCCAGAGG GCAGCCGCAA GAACCCCGCC CGCACCTGCC GTGACCTCAA GATGTGCCAC
TCTGACTGGA AGAGTGGAGA GTACTGGATT GACCCCAACC AAGGCTGCAA CCTGGATGCC
ATCAAAGTCT TCTGCAACAT GGAGACTGGT GAGACCTGCG TGTACCCCAC TCAGCCCAGT
GTGGCCCAGA AGAACTGGTA CATCAGCAAG AACCCCAAGG ACAAGAGGCA TGTCTGGTTC
GGCGAGAGCA TGACCGATGG ATTCCAGTTC GAGTATGGCG GCCAGGGCTC CGACCCTGCC
GATGTGGCCA TCCAGCTGAC CTTCCTGCGC CTGATGTCCA CCGAGGCCTC CCAGAACATC
ACCTACCACT GCAAGAACAG CGTGGCCTAC ATGGACCAGC AGACTGGCAA CCTCAAGAAG
GCCCTGCTCC TCCAGGGCTC CAACGAGATC GAGATCCGCG CCGAGGGCAA CAGCCGCTTC
ACCTACAGCG TCACTGTCGA TGGCTGCACG AGTCACACCG GAGCCTGGGG CAAGACAGTG
ATTGAATACA AAACCACCAA GACCTCCCGC CTGCCCATCA TCGATGTGGC CCCCTTGGAC
GTTGGTGCCC CAGACCAGGA ATTCGGCTTC GACGTTGGCC CTGTCTGCTT CCTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25462
Clone ID Related Accession (Same CDS sequence) NM_000088.3
Accession Version NM_000088.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4395bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product collagen alpha-1(I) chain preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC015909.14 and Z74615.1. This sequence is a reference standard in the RefSeqGene project. On Jul 14, 2006 this sequence version replaced NM_000088.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z74615.1, BC036531.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGTTCAGCT TTGTGGACCT CCGGCTCCTG CTCCTCTTAG CGGCCACCGC CCTCCTGACG 
CACGGCCAAG AGGAAGGCCA AGTCGAGGGC CAAGACGAAG ACATCCCACC AATCACCTGC
GTACAGAACG GCCTCAGGTA CCATGACCGA GACGTGTGGA AACCCGAGCC CTGCCGGATC
TGCGTCTGCG ACAACGGCAA GGTGTTGTGC GATGACGTGA TCTGTGACGA GACCAAGAAC
TGCCCCGGCG CCGAAGTCCC CGAGGGCGAG TGCTGTCCCG TCTGCCCCGA CGGCTCAGAG
TCACCCACCG ACCAAGAAAC CACCGGCGTC GAGGGACCCA AGGGAGACAC TGGCCCCCGA
GGCCCAAGGG GACCCGCAGG CCCCCCTGGC CGAGATGGCA TCCCTGGACA GCCTGGACTT
CCCGGACCCC CCGGACCCCC CGGACCTCCC GGACCCCCTG GCCTCGGAGG AAACTTTGCT
CCCCAGCTGT CTTATGGCTA TGATGAGAAA TCAACCGGAG GAATTTCCGT GCCTGGCCCC
ATGGGTCCCT CTGGTCCTCG TGGTCTCCCT GGCCCCCCTG GTGCACCTGG TCCCCAAGGC
TTCCAAGGTC CCCCTGGTGA GCCTGGCGAG CCTGGAGCTT CAGGTCCCAT GGGTCCCCGA
GGTCCCCCAG GTCCCCCTGG AAAGAATGGA GATGATGGGG AAGCTGGAAA ACCTGGTCGT
CCTGGTGAGC GTGGGCCTCC TGGGCCTCAG GGTGCTCGAG GATTGCCCGG AACAGCTGGC
CTCCCTGGAA TGAAGGGACA CAGAGGTTTC AGTGGTTTGG ATGGTGCCAA GGGAGATGCT
GGTCCTGCTG GTCCTAAGGG TGAGCCTGGC AGCCCTGGTG AAAATGGAGC TCCTGGTCAG
ATGGGCCCCC GTGGCCTGCC TGGTGAGAGA GGTCGCCCTG GAGCCCCTGG CCCTGCTGGT
GCTCGTGGAA ATGATGGTGC TACTGGTGCT GCCGGGCCCC CTGGTCCCAC CGGCCCCGCT
GGTCCTCCTG GCTTCCCTGG TGCTGTTGGT GCTAAGGGTG AAGCTGGTCC CCAAGGGCCC
CGAGGCTCTG AAGGTCCCCA GGGTGTGCGT GGTGAGCCTG GCCCCCCTGG CCCTGCTGGT
GCTGCTGGCC CTGCTGGAAA CCCTGGTGCT GATGGACAGC CTGGTGCTAA AGGTGCCAAT
GGTGCTCCTG GTATTGCTGG TGCTCCTGGC TTCCCTGGTG CCCGAGGCCC CTCTGGACCC
CAGGGCCCCG GCGGCCCTCC TGGTCCCAAG GGTAACAGCG GTGAACCTGG TGCTCCTGGC
AGCAAAGGAG ACACTGGTGC TAAGGGAGAG CCTGGCCCTG TTGGTGTTCA AGGACCCCCT
GGCCCTGCTG GAGAGGAAGG AAAGCGAGGA GCTCGAGGTG AACCCGGACC CACTGGCCTG
CCCGGACCCC CTGGCGAGCG TGGTGGACCT GGTAGCCGTG GTTTCCCTGG CGCAGATGGT
GTTGCTGGTC CCAAGGGTCC CGCTGGTGAA CGTGGTTCTC CTGGCCCTGC TGGCCCCAAA
GGATCTCCTG GTGAAGCTGG TCGTCCCGGT GAAGCTGGTC TGCCTGGTGC CAAGGGTCTG
ACTGGAAGCC CTGGCAGCCC TGGTCCTGAT GGCAAAACTG GCCCCCCTGG TCCCGCCGGT
CAAGATGGTC GCCCCGGACC CCCAGGCCCA CCTGGTGCCC GTGGTCAGGC TGGTGTGATG
GGATTCCCTG GACCTAAAGG TGCTGCTGGA GAGCCCGGCA AGGCTGGAGA GCGAGGTGTT
CCCGGACCCC CTGGCGCTGT CGGTCCTGCT GGCAAAGATG GAGAGGCTGG AGCTCAGGGA
CCCCCTGGCC CTGCTGGTCC CGCTGGCGAG AGAGGTGAAC AAGGCCCTGC TGGCTCCCCC
GGATTCCAGG GTCTCCCTGG TCCTGCTGGT CCTCCAGGTG AAGCAGGCAA ACCTGGTGAA
CAGGGTGTTC CTGGAGACCT TGGCGCCCCT GGCCCCTCTG GAGCAAGAGG CGAGAGAGGT
TTCCCTGGCG AGCGTGGTGT GCAAGGTCCC CCTGGTCCTG CTGGTCCCCG AGGGGCCAAC
GGTGCTCCCG GCAACGATGG TGCTAAGGGT GATGCTGGTG CCCCTGGAGC TCCCGGTAGC
CAGGGCGCCC CTGGCCTTCA GGGAATGCCT GGTGAACGTG GTGCAGCTGG TCTTCCAGGG
CCTAAGGGTG ACAGAGGTGA TGCTGGTCCC AAAGGTGCTG ATGGCTCTCC TGGCAAAGAT
GGCGTCCGTG GTCTGACTGG CCCCATTGGT CCTCCTGGCC CTGCTGGTGC CCCTGGTGAC
AAGGGTGAAA GTGGTCCCAG CGGCCCTGCT GGTCCCACTG GAGCTCGTGG TGCCCCCGGA
GACCGTGGTG AGCCTGGTCC CCCCGGCCCT GCTGGCTTTG CTGGCCCCCC TGGTGCTGAC
GGCCAACCTG GTGCTAAAGG CGAACCTGGT GATGCTGGTG CTAAAGGCGA TGCTGGTCCC
CCTGGCCCTG CCGGACCCGC TGGACCCCCT GGCCCCATTG GTAATGTTGG TGCTCCTGGA
GCCAAAGGTG CTCGCGGCAG CGCTGGTCCC CCTGGTGCTA CTGGTTTCCC TGGTGCTGCT
GGCCGAGTCG GTCCTCCTGG CCCCTCTGGA AATGCTGGAC CCCCTGGCCC TCCTGGTCCT
GCTGGCAAAG AAGGCGGCAA AGGTCCCCGT GGTGAGACTG GCCCTGCTGG ACGTCCTGGT
GAAGTTGGTC CCCCTGGTCC CCCTGGCCCT GCTGGCGAGA AAGGATCCCC TGGTGCTGAT
GGTCCTGCTG GTGCTCCTGG TACTCCCGGG CCTCAAGGTA TTGCTGGACA GCGTGGTGTG
GTCGGCCTGC CTGGTCAGAG AGGAGAGAGA GGCTTCCCTG GTCTTCCTGG CCCCTCTGGT
GAACCTGGCA AACAAGGTCC CTCTGGAGCA AGTGGTGAAC GTGGTCCCCC TGGTCCCATG
GGCCCCCCTG GATTGGCTGG ACCCCCTGGT GAATCTGGAC GTGAGGGGGC TCCTGGTGCC
GAAGGTTCCC CTGGACGAGA CGGTTCTCCT GGCGCCAAGG GTGACCGTGG TGAGACCGGC
CCCGCTGGAC CCCCTGGTGC TCCTGGTGCT CCTGGTGCCC CTGGCCCCGT TGGCCCTGCT
GGCAAGAGTG GTGATCGTGG TGAGACTGGT CCTGCTGGTC CCGCCGGTCC TGTCGGCCCT
GTTGGCGCCC GTGGCCCCGC CGGACCCCAA GGCCCCCGTG GTGACAAGGG TGAGACAGGC
GAACAGGGCG ACAGAGGCAT AAAGGGTCAC CGTGGCTTCT CTGGCCTCCA GGGTCCCCCT
GGCCCTCCTG GCTCTCCTGG TGAACAAGGT CCCTCTGGAG CCTCTGGTCC TGCTGGTCCC
CGAGGTCCCC CTGGCTCTGC TGGTGCTCCT GGCAAAGATG GACTCAACGG TCTCCCTGGC
CCCATTGGGC CCCCTGGTCC TCGCGGTCGC ACTGGTGATG CTGGTCCTGT TGGTCCCCCC
GGCCCTCCTG GACCTCCTGG TCCCCCTGGT CCTCCCAGCG CTGGTTTCGA CTTCAGCTTC
CTGCCCCAGC CACCTCAAGA GAAGGCTCAC GATGGTGGCC GCTACTACCG GGCTGATGAT
GCCAATGTGG TTCGTGACCG TGACCTCGAG GTGGACACCA CCCTCAAGAG CCTGAGCCAG
CAGATCGAGA ACATCCGGAG CCCAGAGGGC AGCCGCAAGA ACCCCGCCCG CACCTGCCGT
GACCTCAAGA TGTGCCACTC TGACTGGAAG AGTGGAGAGT ACTGGATTGA CCCCAACCAA
GGCTGCAACC TGGATGCCAT CAAAGTCTTC TGCAACATGG AGACTGGTGA GACCTGCGTG
TACCCCACTC AGCCCAGTGT GGCCCAGAAG AACTGGTACA TCAGCAAGAA CCCCAAGGAC
AAGAGGCATG TCTGGTTCGG CGAGAGCATG ACCGATGGAT TCCAGTTCGA GTATGGCGGC
CAGGGCTCCG ACCCTGCCGA TGTGGCCATC CAGCTGACCT TCCTGCGCCT GATGTCCACC
GAGGCCTCCC AGAACATCAC CTACCACTGC AAGAACAGCG TGGCCTACAT GGACCAGCAG
ACTGGCAACC TCAAGAAGGC CCTGCTCCTC CAGGGCTCCA ACGAGATCGA GATCCGCGCC
GAGGGCAACA GCCGCTTCAC CTACAGCGTC ACTGTCGATG GCTGCACGAG TCACACCGGA
GCCTGGGGCA AGACAGTGAT TGAATACAAA ACCACCAAGA CCTCCCGCCT GCCCATCATC
GATGTGGCCC CCTTGGACGT TGGTGCCCCA GACCAGGAAT TCGGCTTCGA CGTTGGCCCT
GTCTGCTTCC TGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000079.2
CDS127..4521
Misc Feature(1)115..117
Misc Feature(2)244..411
Misc Feature(3)397..399
Misc Feature(4)478..480
Misc Feature(5)607..612
Misc Feature(6)610..660
Misc Feature(7)610..612
Misc Feature(8)634..636
Misc Feature(9)637..639
Misc Feature(10)661..3702
Misc Feature(11)832..1011
Misc Feature(12)919..921
Misc Feature(13)937..939
Misc Feature(14)940..1098
Misc Feature(15)1480..1731
Misc Feature(16)1651..1812
Misc Feature(17)1732..1911
Misc Feature(18)2128..2301
Misc Feature(19)2227..2403
Misc Feature(20)2359..2367
Misc Feature(21)2485..2487
Misc Feature(22)2983..2988
Misc Feature(23)3403..3411
Misc Feature(24)3448..3450
Misc Feature(25)3616..3618
Misc Feature(26)3703..3780
Misc Feature(27)3748..3750
Misc Feature(28)3778..3783
Misc Feature(29)3814..4515
Exon (1)1..229
Exon (2)230..424
Exon (3)425..459
Exon (4)460..495
Exon (5)496..597
Exon (6)598..669
Exon (7)670..714
Exon (8)715..768
Exon (9)769..822
Exon (10)823..876
Exon (11)877..930
Exon (12)931..984
Exon (13)985..1029
Exon (14)1030..1083
Exon (15)1084..1128
Exon (16)1129..1182
Exon (17)1183..1281
Exon (18)1282..1326
Exon (19)1327..1425
Exon (20)1426..1479
Exon (21)1480..1587
Exon (22)1588..1641
Exon (23)1642..1740
Exon (24)1741..1794
Exon (25)1795..1893
Exon (26)1894..1947
Exon (27)1948..2001
Exon (28)2002..2055
Exon (29)2056..2109
Exon (30)2110..2154
Exon (31)2155..2253
Exon (32)2254..2361
Exon (33)2362..2469
Exon (34)2470..2523
Exon (35)2524..2577
Exon (36)2578..2685
Exon (37)2686..2739
Exon (38)2740..2793
Exon (39)2794..2955
Exon (40)2956..3063
Exon (41)3064..3171
Exon (42)3172..3225
Exon (43)3226..3333
Exon (44)3334..3387
Exon (45)3388..3495
Exon (46)3496..3549
Exon (47)3550..3657
Exon (48)3658..3940
Exon (49)3941..4131
Exon (50)4132..4374
Exon (51)4375..5927
Translation

Target ORF information:

RefSeq Version NM_000088.3
Organism Homo sapiens(Human)
Definition Homo sapiens collagen type I alpha 1 chain (COL1A1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000088.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGTTCAGCT TTGTGGACCT CCGGCTCCTG CTCCTCTTAG CGGCCACCGC CCTCCTGACG 
CACGGCCAAG AGGAAGGCCA AGTCGAGGGC CAAGACGAAG ACATCCCACC AATCACCTGC
GTACAGAACG GCCTCAGGTA CCATGACCGA GACGTGTGGA AACCCGAGCC CTGCCGGATC
TGCGTCTGCG ACAACGGCAA GGTGTTGTGC GATGACGTGA TCTGTGACGA GACCAAGAAC
TGCCCCGGCG CCGAAGTCCC CGAGGGCGAG TGCTGTCCCG TCTGCCCCGA CGGCTCAGAG
TCACCCACCG ACCAAGAAAC CACCGGCGTC GAGGGACCCA AGGGAGACAC TGGCCCCCGA
GGCCCAAGGG GACCCGCAGG CCCCCCTGGC CGAGATGGCA TCCCTGGACA GCCTGGACTT
CCCGGACCCC CCGGACCCCC CGGACCTCCC GGACCCCCTG GCCTCGGAGG AAACTTTGCT
CCCCAGCTGT CTTATGGCTA TGATGAGAAA TCAACCGGAG GAATTTCCGT GCCTGGCCCC
ATGGGTCCCT CTGGTCCTCG TGGTCTCCCT GGCCCCCCTG GTGCACCTGG TCCCCAAGGC
TTCCAAGGTC CCCCTGGTGA GCCTGGCGAG CCTGGAGCTT CAGGTCCCAT GGGTCCCCGA
GGTCCCCCAG GTCCCCCTGG AAAGAATGGA GATGATGGGG AAGCTGGAAA ACCTGGTCGT
CCTGGTGAGC GTGGGCCTCC TGGGCCTCAG GGTGCTCGAG GATTGCCCGG AACAGCTGGC
CTCCCTGGAA TGAAGGGACA CAGAGGTTTC AGTGGTTTGG ATGGTGCCAA GGGAGATGCT
GGTCCTGCTG GTCCTAAGGG TGAGCCTGGC AGCCCTGGTG AAAATGGAGC TCCTGGTCAG
ATGGGCCCCC GTGGCCTGCC TGGTGAGAGA GGTCGCCCTG GAGCCCCTGG CCCTGCTGGT
GCTCGTGGAA ATGATGGTGC TACTGGTGCT GCCGGGCCCC CTGGTCCCAC CGGCCCCGCT
GGTCCTCCTG GCTTCCCTGG TGCTGTTGGT GCTAAGGGTG AAGCTGGTCC CCAAGGGCCC
CGAGGCTCTG AAGGTCCCCA GGGTGTGCGT GGTGAGCCTG GCCCCCCTGG CCCTGCTGGT
GCTGCTGGCC CTGCTGGAAA CCCTGGTGCT GATGGACAGC CTGGTGCTAA AGGTGCCAAT
GGTGCTCCTG GTATTGCTGG TGCTCCTGGC TTCCCTGGTG CCCGAGGCCC CTCTGGACCC
CAGGGCCCCG GCGGCCCTCC TGGTCCCAAG GGTAACAGCG GTGAACCTGG TGCTCCTGGC
AGCAAAGGAG ACACTGGTGC TAAGGGAGAG CCTGGCCCTG TTGGTGTTCA AGGACCCCCT
GGCCCTGCTG GAGAGGAAGG AAAGCGAGGA GCTCGAGGTG AACCCGGACC CACTGGCCTG
CCCGGACCCC CTGGCGAGCG TGGTGGACCT GGTAGCCGTG GTTTCCCTGG CGCAGATGGT
GTTGCTGGTC CCAAGGGTCC CGCTGGTGAA CGTGGTTCTC CTGGCCCTGC TGGCCCCAAA
GGATCTCCTG GTGAAGCTGG TCGTCCCGGT GAAGCTGGTC TGCCTGGTGC CAAGGGTCTG
ACTGGAAGCC CTGGCAGCCC TGGTCCTGAT GGCAAAACTG GCCCCCCTGG TCCCGCCGGT
CAAGATGGTC GCCCCGGACC CCCAGGCCCA CCTGGTGCCC GTGGTCAGGC TGGTGTGATG
GGATTCCCTG GACCTAAAGG TGCTGCTGGA GAGCCCGGCA AGGCTGGAGA GCGAGGTGTT
CCCGGACCCC CTGGCGCTGT CGGTCCTGCT GGCAAAGATG GAGAGGCTGG AGCTCAGGGA
CCCCCTGGCC CTGCTGGTCC CGCTGGCGAG AGAGGTGAAC AAGGCCCTGC TGGCTCCCCC
GGATTCCAGG GTCTCCCTGG TCCTGCTGGT CCTCCAGGTG AAGCAGGCAA ACCTGGTGAA
CAGGGTGTTC CTGGAGACCT TGGCGCCCCT GGCCCCTCTG GAGCAAGAGG CGAGAGAGGT
TTCCCTGGCG AGCGTGGTGT GCAAGGTCCC CCTGGTCCTG CTGGTCCCCG AGGGGCCAAC
GGTGCTCCCG GCAACGATGG TGCTAAGGGT GATGCTGGTG CCCCTGGAGC TCCCGGTAGC
CAGGGCGCCC CTGGCCTTCA GGGAATGCCT GGTGAACGTG GTGCAGCTGG TCTTCCAGGG
CCTAAGGGTG ACAGAGGTGA TGCTGGTCCC AAAGGTGCTG ATGGCTCTCC TGGCAAAGAT
GGCGTCCGTG GTCTGACTGG CCCCATTGGT CCTCCTGGCC CTGCTGGTGC CCCTGGTGAC
AAGGGTGAAA GTGGTCCCAG CGGCCCTGCT GGTCCCACTG GAGCTCGTGG TGCCCCCGGA
GACCGTGGTG AGCCTGGTCC CCCCGGCCCT GCTGGCTTTG CTGGCCCCCC TGGTGCTGAC
GGCCAACCTG GTGCTAAAGG CGAACCTGGT GATGCTGGTG CTAAAGGCGA TGCTGGTCCC
CCTGGCCCTG CCGGACCCGC TGGACCCCCT GGCCCCATTG GTAATGTTGG TGCTCCTGGA
GCCAAAGGTG CTCGCGGCAG CGCTGGTCCC CCTGGTGCTA CTGGTTTCCC TGGTGCTGCT
GGCCGAGTCG GTCCTCCTGG CCCCTCTGGA AATGCTGGAC CCCCTGGCCC TCCTGGTCCT
GCTGGCAAAG AAGGCGGCAA AGGTCCCCGT GGTGAGACTG GCCCTGCTGG ACGTCCTGGT
GAAGTTGGTC CCCCTGGTCC CCCTGGCCCT GCTGGCGAGA AAGGATCCCC TGGTGCTGAT
GGTCCTGCTG GTGCTCCTGG TACTCCCGGG CCTCAAGGTA TTGCTGGACA GCGTGGTGTG
GTCGGCCTGC CTGGTCAGAG AGGAGAGAGA GGCTTCCCTG GTCTTCCTGG CCCCTCTGGT
GAACCTGGCA AACAAGGTCC CTCTGGAGCA AGTGGTGAAC GTGGTCCCCC TGGTCCCATG
GGCCCCCCTG GATTGGCTGG ACCCCCTGGT GAATCTGGAC GTGAGGGGGC TCCTGGTGCC
GAAGGTTCCC CTGGACGAGA CGGTTCTCCT GGCGCCAAGG GTGACCGTGG TGAGACCGGC
CCCGCTGGAC CCCCTGGTGC TCCTGGTGCT CCTGGTGCCC CTGGCCCCGT TGGCCCTGCT
GGCAAGAGTG GTGATCGTGG TGAGACTGGT CCTGCTGGTC CCGCCGGTCC TGTCGGCCCT
GTTGGCGCCC GTGGCCCCGC CGGACCCCAA GGCCCCCGTG GTGACAAGGG TGAGACAGGC
GAACAGGGCG ACAGAGGCAT AAAGGGTCAC CGTGGCTTCT CTGGCCTCCA GGGTCCCCCT
GGCCCTCCTG GCTCTCCTGG TGAACAAGGT CCCTCTGGAG CCTCTGGTCC TGCTGGTCCC
CGAGGTCCCC CTGGCTCTGC TGGTGCTCCT GGCAAAGATG GACTCAACGG TCTCCCTGGC
CCCATTGGGC CCCCTGGTCC TCGCGGTCGC ACTGGTGATG CTGGTCCTGT TGGTCCCCCC
GGCCCTCCTG GACCTCCTGG TCCCCCTGGT CCTCCCAGCG CTGGTTTCGA CTTCAGCTTC
CTGCCCCAGC CACCTCAAGA GAAGGCTCAC GATGGTGGCC GCTACTACCG GGCTGATGAT
GCCAATGTGG TTCGTGACCG TGACCTCGAG GTGGACACCA CCCTCAAGAG CCTGAGCCAG
CAGATCGAGA ACATCCGGAG CCCAGAGGGC AGCCGCAAGA ACCCCGCCCG CACCTGCCGT
GACCTCAAGA TGTGCCACTC TGACTGGAAG AGTGGAGAGT ACTGGATTGA CCCCAACCAA
GGCTGCAACC TGGATGCCAT CAAAGTCTTC TGCAACATGG AGACTGGTGA GACCTGCGTG
TACCCCACTC AGCCCAGTGT GGCCCAGAAG AACTGGTACA TCAGCAAGAA CCCCAAGGAC
AAGAGGCATG TCTGGTTCGG CGAGAGCATG ACCGATGGAT TCCAGTTCGA GTATGGCGGC
CAGGGCTCCG ACCCTGCCGA TGTGGCCATC CAGCTGACCT TCCTGCGCCT GATGTCCACC
GAGGCCTCCC AGAACATCAC CTACCACTGC AAGAACAGCG TGGCCTACAT GGACCAGCAG
ACTGGCAACC TCAAGAAGGC CCTGCTCCTC CAGGGCTCCA ACGAGATCGA GATCCGCGCC
GAGGGCAACA GCCGCTTCAC CTACAGCGTC ACTGTCGATG GCTGCACGAG TCACACCGGA
GCCTGGGGCA AGACAGTGAT TGAATACAAA ACCACCAAGA CCTCCCGCCT GCCCATCATC
GATGTGGCCC CCTTGGACGT TGGTGCCCCA GACCAGGAAT TCGGCTTCGA CGTTGGCCCT
GTCTGCTTCC TGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50114
Clone ID Related Accession (Same CDS sequence) XM_005257058.3
Accession Version XM_005257058.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product collagen alpha-1(I) chain isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005257058.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
ATGTTCAGCT TTGTGGACCT CCGGCTCCTG CTCCTCTTAG CGGCCACCGC CCTCCTGACG 
CACGGCCAAG AGGAAGGCCA AGTCGAGGGC CAAGACGAAG ACATCCCACC AATCACCTGC
GTACAGAACG GCCTCAGGTA CCATGACCGA GACGTGTGGA AACCCGAGCC CTGCCGGATC
TGCGTCTGCG ACAACGGCAA GGTGTTGTGC GATGACGTGA TCTGTGACGA GACCAAGAAC
TGCCCCGGCG CCGAAGTCCC CGAGGGCGAG TGCTGTCCCG TCTGCCCCGA CGGCTCAGAG
TCACCCACCG ACCAAGAAAC CACCGGCGTC GAGGGACCCA AGGGAGACAC TGGCCCCCGA
GGCCCAAGGG GACCCGCAGG CCCCCCTGGC CGAGATGGCA TCCCTGGACA GCCTGGACTT
CCCGGACCCC CCGGACCCCC CGGACCTCCC GGACCCCCTG GCCTCGGAGG AAACTTTGCT
CCCCAGCTGT CTTATGGCTA TGATGAGAAA TCAACCGGAG GAATTTCCGT GCCTGGCCCC
ATGGGTCCCT CTGGTCCTCG TGGTCTCCCT GGCCCCCCTG GTGCACCTGG TCCCCAAGGC
TTCCAAGGTC CCCCTGGTGA GCCTGGCGAG CCTGGAGCTT CAGGTCCCAT GGGTCCCCGA
GGTCCCCCAG GTCCCCCTGG AAAGAATGGA GATGATGGGG AAGCTGGAAA ACCTGGTCGT
CCTGGTGAGC GTGGGCCTCC TGGGCCTCAG GGTGCTCGAG GATTGCCCGG AACAGCTGGC
CTCCCTGGAA TGAAGGGACA CAGAGGTTTC AGTGGTTTGG ATGGTGCCAA GGGAGATGCT
GGTCCTGCTG GTCCTAAGGG TGAGCCTGGC AGCCCTGGTG AAAATGGAGC TCCTGGTCAG
ATGGGCCCCC GTGGCCTGCC TGGTGAGAGA GGTCGCCCTG GAGCCCCTGG CCCTGCTGGT
GCTCGTGGAA ATGATGGTGC TACTGGTGCT GCCGGGCCCC CTGGTCCCAC CGGCCCCGCT
GGTCCTCCTG GCTTCCCTGG TGCTGTTGGT GCTAAGGGTG AAGCTGGTCC CCAAGGGCCC
CGAGGCTCTG AAGGTCCCCA GGGTGTGCGT GGTGAGCCTG GCCCCCCTGG CCCTGCTGGT
GCTGCTGGCC CTGCTGGAAA CCCTGGTGCT GATGGACAGC CTGGTGCTAA AGGTGCCAAT
GGTGCTCCTG GTATTGCTGG TGCTCCTGGC TTCCCTGGTG CCCGAGGCCC CTCTGGACCC
CAGGGCCCCG GCGGCCCTCC TGGTCCCAAG GGTAACAGCG GTGAACCTGG TGCTCCTGGC
AGCAAAGGAG ACACTGGTGC TAAGGGAGAG CCTGGCCCTG TTGGTGTTCA AGGACCCCCT
GGCCCTGCTG GAGAGGAAGG AAAGCGAGGA GCTCGAGGTG AACCCGGACC CACTGGCCTG
CCCGGACCCC CTGGCGAGCG TGGTGGACCT GGTAGCCGTG GTTTCCCTGG CGCAGATGGT
GTTGCTGGTC CCAAGGGTCC CGCTGGTGAA CGTGGTTCTC CTGGCCCTGC TGGCCCCAAA
GGATCTCCTG GTGAAGCTGG TCGTCCCGGT GAAGCTGGTC TGCCTGGTGC CAAGGGTCTG
ACTGGAAGCC CTGGCAGCCC TGGTCCTGAT GGCAAAACTG GCCCCCCTGG TCCCGCCGGT
CAAGATGGTC GCCCCGGACC CCCAGGCCCA CCTGGTGCCC GTGGTCAGGC TGGTGTGATG
GGATTCCCTG GACCTAAAGG TGCTGCTGGA GAGCCCGGCA AGGCTGGAGA GCGAGGTGTT
CCCGGACCCC CTGGCGCTGT CGGTCCTGCT GGCAAAGATG GAGAGGCTGG AGCTCAGGGA
CCCCCTGGCC CTGCTGGTCC CGCTGGCGAG AGAGGTGAAC AAGGCCCTGC TGGCTCCCCC
GGATTCCAGG GTCTCCCTGG TCCTGCTGGT CCTCCAGGTG AAGCAGGCAA ACCTGGTGAA
CAGGGTGTTC CTGGAGACCT TGGCGCCCCT GGCCCCTCTG GAGCAAGAGG CGAGAGAGGT
TTCCCTGGCG AGCGTGGTGT GCAAGGTCCC CCTGGTCCTG CTGGTCCCCG AGGGGCCAAC
GGTGCTCCCG GCAACGATGG TGCTAAGGGT GATGCTGGTG CCCCTGGAGC TCCCGGTAGC
CAGGGCGCCC CTGGCCTTCA GGGAATGCCT GGTGAACGTG GTGCAGCTGG TCTTCCAGGG
CCTAAGGGTG ACAGAGGTGA TGCTGGTCCC AAAGGTGCTG ATGGCTCTCC TGGCAAAGAT
GGCGTCCGTG GTCTGACTGG CCCCATTGGT CCTCCTGGCC CTGCTGGTGC CCCTGGTGAC
AAGGGTGAAA GTGGTCCCAG CGGCCCTGCT GGTCCCACTG GAGCTCGTGG TGCCCCCGGA
GACCGTGGTG AGCCTGGTCC CCCCGGCCCT GCTGGCTTTG CTGGCCCCCC TGGTGCTGAC
GGCCAACCTG GTGCTAAAGG CGAACCTGGT GATGCTGGTG CTAAAGGCGA TGCTGGTCCC
CCTGGCCCTG CCGGACCCGC TGGACCCCCT GGCCCCATTG GTAATGTTGG TGCTCCTGGA
GCCAAAGGTG CTCGCGGCAG CGCTGGTCCC CCTGGTGCTA CTGGTTTCCC TGGTGCTGCT
GGCCGAGTCG GTCCTCCTGG CCCCTCTGGT GAACCTGGCA AACAAGGTCC CTCTGGAGCA
AGTGGTGAAC GTGGTCCCCC TGGTCCCATG GGCCCCCCTG GATTGGCTGG ACCCCCTGGT
GAATCTGGAC GTGAGGGGGC TCCTGGTGCC GAAGGTTCCC CTGGACGAGA CGGTTCTCCT
GGCGCCAAGG GTGACCGTGG TGAGACCGGC CCCGCTGGAC CCCCTGGTGC TCCTGGTGCT
CCTGGTGCCC CTGGCCCCGT TGGCCCTGCT GGCAAGAGTG GTGATCGTGG TGAGACTGGT
CCTGCTGGTC CCGCCGGTCC TGTCGGCCCT GTTGGCGCCC GTGGCCCCGC CGGACCCCAA
GGCCCCCGTG GTGACAAGGG TGAGACAGGC GAACAGGGCG ACAGAGGCAT AAAGGGTCAC
CGTGGCTTCT CTGGCCTCCA GGGTCCCCCT GGCCCTCCTG GCTCTCCTGG TGAACAAGGT
CCCTCTGGAG CCTCTGGTCC TGCTGGTCCC CGAGGTCCCC CTGGCTCTGC TGGTGCTCCT
GGCAAAGATG GACTCAACGG TCTCCCTGGC CCCATTGGGC CCCCTGGTCC TCGCGGTCGC
ACTGGTGATG CTGGTCCTGT TGGTCCCCCC GGCCCTCCTG GACCTCCTGG TCCCCCTGGT
CCTCCCAGCG CTGGTTTCGA CTTCAGCTTC CTGCCCCAGC CACCTCAAGA GAAGGCTCAC
GATGGTGGCC GCTACTACCG GGCTGATGAT GCCAATGTGG TTCGTGACCG TGACCTCGAG
GTGGACACCA CCCTCAAGAG CCTGAGCCAG CAGATCGAGA ACATCCGGAG CCCAGAGGGC
AGCCGCAAGA ACCCCGCCCG CACCTGCCGT GACCTCAAGA TGTGCCACTC TGACTGGAAG
AGTGGAGAGT ACTGGATTGA CCCCAACCAA GGCTGCAACC TGGATGCCAT CAAAGTCTTC
TGCAACATGG AGACTGGTGA GACCTGCGTG TACCCCACTC AGCCCAGTGT GGCCCAGAAG
AACTGGTACA TCAGCAAGAA CCCCAAGGAC AAGAGGCATG TCTGGTTCGG CGAGAGCATG
ACCGATGGAT TCCAGTTCGA GTATGGCGGC CAGGGCTCCG ACCCTGCCGA TGTGGCCATC
CAGCTGACCT TCCTGCGCCT GATGTCCACC GAGGCCTCCC AGAACATCAC CTACCACTGC
AAGAACAGCG TGGCCTACAT GGACCAGCAG ACTGGCAACC TCAAGAAGGC CCTGCTCCTC
CAGGGCTCCA ACGAGATCGA GATCCGCGCC GAGGGCAACA GCCGCTTCAC CTACAGCGTC
ACTGTCGATG GCTGCACGAG TCACACCGGA GCCTGGGGCA AGACAGTGAT TGAATACAAA
ACCACCAAGA CCTCCCGCCT GCCCATCATC GATGTGGCCC CCTTGGACGT TGGTGCCCCA
GACCAGGAAT TCGGCTTCGA CGTTGGCCCT GTCTGCTTCC TGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005257115.2
CDS136..4260
Misc Feature(1)253..420
Misc Feature(2)841..1020
Misc Feature(3)949..1107
Misc Feature(4)1489..1740
Misc Feature(5)1660..1821
Misc Feature(6)1741..1956
Misc Feature(7)2137..2310
Misc Feature(8)2236..2412
Misc Feature(9)3553..4254
Translation

Target ORF information:

RefSeq Version XM_005257058.3
Organism Homo sapiens(Human)
Definition Homo sapiens collagen type I alpha 1 (COL1A1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257058.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
ATGTTCAGCT TTGTGGACCT CCGGCTCCTG CTCCTCTTAG CGGCCACCGC CCTCCTGACG 
CACGGCCAAG AGGAAGGCCA AGTCGAGGGC CAAGACGAAG ACATCCCACC AATCACCTGC
GTACAGAACG GCCTCAGGTA CCATGACCGA GACGTGTGGA AACCCGAGCC CTGCCGGATC
TGCGTCTGCG ACAACGGCAA GGTGTTGTGC GATGACGTGA TCTGTGACGA GACCAAGAAC
TGCCCCGGCG CCGAAGTCCC CGAGGGCGAG TGCTGTCCCG TCTGCCCCGA CGGCTCAGAG
TCACCCACCG ACCAAGAAAC CACCGGCGTC GAGGGACCCA AGGGAGACAC TGGCCCCCGA
GGCCCAAGGG GACCCGCAGG CCCCCCTGGC CGAGATGGCA TCCCTGGACA GCCTGGACTT
CCCGGACCCC CCGGACCCCC CGGACCTCCC GGACCCCCTG GCCTCGGAGG AAACTTTGCT
CCCCAGCTGT CTTATGGCTA TGATGAGAAA TCAACCGGAG GAATTTCCGT GCCTGGCCCC
ATGGGTCCCT CTGGTCCTCG TGGTCTCCCT GGCCCCCCTG GTGCACCTGG TCCCCAAGGC
TTCCAAGGTC CCCCTGGTGA GCCTGGCGAG CCTGGAGCTT CAGGTCCCAT GGGTCCCCGA
GGTCCCCCAG GTCCCCCTGG AAAGAATGGA GATGATGGGG AAGCTGGAAA ACCTGGTCGT
CCTGGTGAGC GTGGGCCTCC TGGGCCTCAG GGTGCTCGAG GATTGCCCGG AACAGCTGGC
CTCCCTGGAA TGAAGGGACA CAGAGGTTTC AGTGGTTTGG ATGGTGCCAA GGGAGATGCT
GGTCCTGCTG GTCCTAAGGG TGAGCCTGGC AGCCCTGGTG AAAATGGAGC TCCTGGTCAG
ATGGGCCCCC GTGGCCTGCC TGGTGAGAGA GGTCGCCCTG GAGCCCCTGG CCCTGCTGGT
GCTCGTGGAA ATGATGGTGC TACTGGTGCT GCCGGGCCCC CTGGTCCCAC CGGCCCCGCT
GGTCCTCCTG GCTTCCCTGG TGCTGTTGGT GCTAAGGGTG AAGCTGGTCC CCAAGGGCCC
CGAGGCTCTG AAGGTCCCCA GGGTGTGCGT GGTGAGCCTG GCCCCCCTGG CCCTGCTGGT
GCTGCTGGCC CTGCTGGAAA CCCTGGTGCT GATGGACAGC CTGGTGCTAA AGGTGCCAAT
GGTGCTCCTG GTATTGCTGG TGCTCCTGGC TTCCCTGGTG CCCGAGGCCC CTCTGGACCC
CAGGGCCCCG GCGGCCCTCC TGGTCCCAAG GGTAACAGCG GTGAACCTGG TGCTCCTGGC
AGCAAAGGAG ACACTGGTGC TAAGGGAGAG CCTGGCCCTG TTGGTGTTCA AGGACCCCCT
GGCCCTGCTG GAGAGGAAGG AAAGCGAGGA GCTCGAGGTG AACCCGGACC CACTGGCCTG
CCCGGACCCC CTGGCGAGCG TGGTGGACCT GGTAGCCGTG GTTTCCCTGG CGCAGATGGT
GTTGCTGGTC CCAAGGGTCC CGCTGGTGAA CGTGGTTCTC CTGGCCCTGC TGGCCCCAAA
GGATCTCCTG GTGAAGCTGG TCGTCCCGGT GAAGCTGGTC TGCCTGGTGC CAAGGGTCTG
ACTGGAAGCC CTGGCAGCCC TGGTCCTGAT GGCAAAACTG GCCCCCCTGG TCCCGCCGGT
CAAGATGGTC GCCCCGGACC CCCAGGCCCA CCTGGTGCCC GTGGTCAGGC TGGTGTGATG
GGATTCCCTG GACCTAAAGG TGCTGCTGGA GAGCCCGGCA AGGCTGGAGA GCGAGGTGTT
CCCGGACCCC CTGGCGCTGT CGGTCCTGCT GGCAAAGATG GAGAGGCTGG AGCTCAGGGA
CCCCCTGGCC CTGCTGGTCC CGCTGGCGAG AGAGGTGAAC AAGGCCCTGC TGGCTCCCCC
GGATTCCAGG GTCTCCCTGG TCCTGCTGGT CCTCCAGGTG AAGCAGGCAA ACCTGGTGAA
CAGGGTGTTC CTGGAGACCT TGGCGCCCCT GGCCCCTCTG GAGCAAGAGG CGAGAGAGGT
TTCCCTGGCG AGCGTGGTGT GCAAGGTCCC CCTGGTCCTG CTGGTCCCCG AGGGGCCAAC
GGTGCTCCCG GCAACGATGG TGCTAAGGGT GATGCTGGTG CCCCTGGAGC TCCCGGTAGC
CAGGGCGCCC CTGGCCTTCA GGGAATGCCT GGTGAACGTG GTGCAGCTGG TCTTCCAGGG
CCTAAGGGTG ACAGAGGTGA TGCTGGTCCC AAAGGTGCTG ATGGCTCTCC TGGCAAAGAT
GGCGTCCGTG GTCTGACTGG CCCCATTGGT CCTCCTGGCC CTGCTGGTGC CCCTGGTGAC
AAGGGTGAAA GTGGTCCCAG CGGCCCTGCT GGTCCCACTG GAGCTCGTGG TGCCCCCGGA
GACCGTGGTG AGCCTGGTCC CCCCGGCCCT GCTGGCTTTG CTGGCCCCCC TGGTGCTGAC
GGCCAACCTG GTGCTAAAGG CGAACCTGGT GATGCTGGTG CTAAAGGCGA TGCTGGTCCC
CCTGGCCCTG CCGGACCCGC TGGACCCCCT GGCCCCATTG GTAATGTTGG TGCTCCTGGA
GCCAAAGGTG CTCGCGGCAG CGCTGGTCCC CCTGGTGCTA CTGGTTTCCC TGGTGCTGCT
GGCCGAGTCG GTCCTCCTGG CCCCTCTGGT GAACCTGGCA AACAAGGTCC CTCTGGAGCA
AGTGGTGAAC GTGGTCCCCC TGGTCCCATG GGCCCCCCTG GATTGGCTGG ACCCCCTGGT
GAATCTGGAC GTGAGGGGGC TCCTGGTGCC GAAGGTTCCC CTGGACGAGA CGGTTCTCCT
GGCGCCAAGG GTGACCGTGG TGAGACCGGC CCCGCTGGAC CCCCTGGTGC TCCTGGTGCT
CCTGGTGCCC CTGGCCCCGT TGGCCCTGCT GGCAAGAGTG GTGATCGTGG TGAGACTGGT
CCTGCTGGTC CCGCCGGTCC TGTCGGCCCT GTTGGCGCCC GTGGCCCCGC CGGACCCCAA
GGCCCCCGTG GTGACAAGGG TGAGACAGGC GAACAGGGCG ACAGAGGCAT AAAGGGTCAC
CGTGGCTTCT CTGGCCTCCA GGGTCCCCCT GGCCCTCCTG GCTCTCCTGG TGAACAAGGT
CCCTCTGGAG CCTCTGGTCC TGCTGGTCCC CGAGGTCCCC CTGGCTCTGC TGGTGCTCCT
GGCAAAGATG GACTCAACGG TCTCCCTGGC CCCATTGGGC CCCCTGGTCC TCGCGGTCGC
ACTGGTGATG CTGGTCCTGT TGGTCCCCCC GGCCCTCCTG GACCTCCTGG TCCCCCTGGT
CCTCCCAGCG CTGGTTTCGA CTTCAGCTTC CTGCCCCAGC CACCTCAAGA GAAGGCTCAC
GATGGTGGCC GCTACTACCG GGCTGATGAT GCCAATGTGG TTCGTGACCG TGACCTCGAG
GTGGACACCA CCCTCAAGAG CCTGAGCCAG CAGATCGAGA ACATCCGGAG CCCAGAGGGC
AGCCGCAAGA ACCCCGCCCG CACCTGCCGT GACCTCAAGA TGTGCCACTC TGACTGGAAG
AGTGGAGAGT ACTGGATTGA CCCCAACCAA GGCTGCAACC TGGATGCCAT CAAAGTCTTC
TGCAACATGG AGACTGGTGA GACCTGCGTG TACCCCACTC AGCCCAGTGT GGCCCAGAAG
AACTGGTACA TCAGCAAGAA CCCCAAGGAC AAGAGGCATG TCTGGTTCGG CGAGAGCATG
ACCGATGGAT TCCAGTTCGA GTATGGCGGC CAGGGCTCCG ACCCTGCCGA TGTGGCCATC
CAGCTGACCT TCCTGCGCCT GATGTCCACC GAGGCCTCCC AGAACATCAC CTACCACTGC
AAGAACAGCG TGGCCTACAT GGACCAGCAG ACTGGCAACC TCAAGAAGGC CCTGCTCCTC
CAGGGCTCCA ACGAGATCGA GATCCGCGCC GAGGGCAACA GCCGCTTCAC CTACAGCGTC
ACTGTCGATG GCTGCACGAG TCACACCGGA GCCTGGGGCA AGACAGTGAT TGAATACAAA
ACCACCAAGA CCTCCCGCCT GCCCATCATC GATGTGGCCC CCTTGGACGT TGGTGCCCCA
GACCAGGAAT TCGGCTTCGA CGTTGGCCCT GTCTGCTTCC TGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56962
Clone ID Related Accession (Same CDS sequence) XM_011524341.1
Accession Version XM_011524341.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4197bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product collagen alpha-1(I) chain isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
ATGTTCAGCT TTGTGGACCT CCGGCTCCTG CTCCTCTTAG CGGCCACCGC CCTCCTGACG 
CACGGCCAAG AGGAAGGCCA AGTCGAGGGC CAAGACGAAG ACATCCCACC AATCACCTGC
GTACAGAACG GCCTCAGGTA CCATGACCGA GACGTGTGGA AACCCGAGCC CTGCCGGATC
TGCGTCTGCG ACAACGGCAA GGTGTTGTGC GATGACGTGA TCTGTGACGA GACCAAGAAC
TGCCCCGGCG CCGAAGTCCC CGAGGGCGAG TGCTGTCCCG TCTGCCCCGA CGGCTCAGAG
TCACCCACCG ACCAAGAAAC CACCGGCGTC GAGGGACCCA AGGGAGACAC TGGCCCCCGA
GGCCCAAGGG GACCCGCAGG CCCCCCTGGC CGAGATGGCA TCCCTGGACA GCCTGGACTT
CCCGGACCCC CCGGACCCCC CGGACCTCCC GGACCCCCTG GCCTCGGAGG AAACTTTGCT
CCCCAGCTGT CTTATGGCTA TGATGAGAAA TCAACCGGAG GAATTTCCGT GCCTGGCCCC
ATGGGTCCCT CTGGTCCTCG TGGTCTCCCT GGCCCCCCTG GTGCACCTGG TCCCCAAGGC
TTCCAAGGTC CCCCTGGTGA GCCTGGCGAG CCTGGAGCTT CAGGTCCCAT GGGTCCCCGA
GGTCCCCCAG GTCCCCCTGG AAAGAATGGA GATGATGGGG AAGCTGGAAA ACCTGGTCGT
CCTGGTGAGC GTGGGCCTCC TGGGCCTCAG GGTGCTCGAG GATTGCCCGG AACAGCTGGC
CTCCCTGGAA TGAAGGGACA CAGAGGTTTC AGTGGTTTGG ATGGTGCCAA GGGAGATGCT
GGTCCTGCTG GTCCTAAGGG TGAGCCTGGC AGCCCTGGTG AAAATGGAGC TCCTGGTCAG
ATGGGCCCCC GTGGCCTGCC TGGTGAGAGA GGTCGCCCTG GAGCCCCTGG CCCTGCTGGA
AACCCTGGTG CTGATGGACA GCCTGGTGCT AAAGGTGCCA ATGGTGCTCC TGGTATTGCT
GGTGCTCCTG GCTTCCCTGG TGCCCGAGGC CCCTCTGGAC CCCAGGGCCC CGGCGGCCCT
CCTGGTCCCA AGGGTAACAG CGGTGAACCT GGTGCTCCTG GCAGCAAAGG AGACACTGGT
GCTAAGGGAG AGCCTGGCCC TGTTGGTGTT CAAGGACCCC CTGGCCCTGC TGGAGAGGAA
GGAAAGCGAG GAGCTCGAGG TGAACCCGGA CCCACTGGCC TGCCCGGACC CCCTGGCGAG
CGTGGTGGAC CTGGTAGCCG TGGTTTCCCT GGCGCAGATG GTGTTGCTGG TCCCAAGGGT
CCCGCTGGTG AACGTGGTTC TCCTGGCCCT GCTGGCCCCA AAGGATCTCC TGGTGAAGCT
GGTCGTCCCG GTGAAGCTGG TCTGCCTGGT GCCAAGGGTC TGACTGGAAG CCCTGGCAGC
CCTGGTCCTG ATGGCAAAAC TGGCCCCCCT GGTCCCGCCG GTCAAGATGG TCGCCCCGGA
CCCCCAGGCC CACCTGGTGC CCGTGGTCAG GCTGGTGTGA TGGGATTCCC TGGACCTAAA
GGTGCTGCTG GAGAGCCCGG CAAGGCTGGA GAGCGAGGTG TTCCCGGACC CCCTGGCGCT
GTCGGTCCTG CTGGCAAAGA TGGAGAGGCT GGAGCTCAGG GACCCCCTGG CCCTGCTGGT
CCCGCTGGCG AGAGAGGTGA ACAAGGCCCT GCTGGCTCCC CCGGATTCCA GGGTCTCCCT
GGTCCTGCTG GTCCTCCAGG TGAAGCAGGC AAACCTGGTG AACAGGGTGT TCCTGGAGAC
CTTGGCGCCC CTGGCCCCTC TGGAGCAAGA GGCGAGAGAG GTTTCCCTGG CGAGCGTGGT
GTGCAAGGTC CCCCTGGTCC TGCTGGTCCC CGAGGGGCCA ACGGTGCTCC CGGCAACGAT
GGTGCTAAGG GTGATGCTGG TGCCCCTGGA GCTCCCGGTA GCCAGGGCGC CCCTGGCCTT
CAGGGAATGC CTGGTGAACG TGGTGCAGCT GGTCTTCCAG GGCCTAAGGG TGACAGAGGT
GATGCTGGTC CCAAAGGTGC TGATGGCTCT CCTGGCAAAG ATGGCGTCCG TGGTCTGACT
GGCCCCATTG GTCCTCCTGG CCCTGCTGGT GCCCCTGGTG ACAAGGGTGA AAGTGGTCCC
AGCGGCCCTG CTGGTCCCAC TGGAGCTCGT GGTGCCCCCG GAGACCGTGG TGAGCCTGGT
CCCCCCGGCC CTGCTGGCTT TGCTGGCCCC CCTGGTGCTG ACGGCCAACC TGGTGCTAAA
GGCGAACCTG GTGATGCTGG TGCTAAAGGC GATGCTGGTC CCCCTGGCCC TGCCGGACCC
GCTGGACCCC CTGGCCCCAT TGGTAATGTT GGTGCTCCTG GAGCCAAAGG TGCTCGCGGC
AGCGCTGGTC CCCCTGGTGC TACTGGTTTC CCTGGTGCTG CTGGCCGAGT CGGTCCTCCT
GGCCCCTCTG GAAATGCTGG ACCCCCTGGC CCTCCTGGTC CTGCTGGCAA AGAAGGCGGC
AAAGGTCCCC GTGGTGAGAC TGGCCCTGCT GGACGTCCTG GTGAAGTTGG TCCCCCTGGT
CCCCCTGGCC CTGCTGGCGA GAAAGGATCC CCTGGTGCTG ATGGTCCTGC TGGTGCTCCT
GGTACTCCCG GGCCTCAAGG TATTGCTGGA CAGCGTGGTG TGGTCGGCCT GCCTGGTCAG
AGAGGAGAGA GAGGCTTCCC TGGTCTTCCT GGCCCCTCTG GTGAACCTGG CAAACAAGGT
CCCTCTGGAG CAAGTGGTGA ACGTGGTCCC CCTGGTCCCA TGGGCCCCCC TGGATTGGCT
GGACCCCCTG GTGAATCTGG ACGTGAGGGG GCTCCTGGTG CCGAAGGTTC CCCTGGACGA
GACGGTTCTC CTGGCGCCAA GGGTGACCGT GGTGAGACCG GCCCCGCTGG ACCCCCTGGT
GCTCCTGGTG CTCCTGGTGC CCCTGGCCCC GTTGGCCCTG CTGGCAAGAG TGGTGATCGT
GGTGAGACTG GTCCTGCTGG TCCCGCCGGT CCTGTCGGCC CTGTTGGCGC CCGTGGCCCC
GCCGGACCCC AAGGCCCCCG TGGTGACAAG GGTGAGACAG GCGAACAGGG CGACAGAGGC
ATAAAGGGTC ACCGTGGCTT CTCTGGCCTC CAGGGTCCCC CTGGCCCTCC TGGCTCTCCT
GGTGAACAAG GTCCCTCTGG AGCCTCTGGT CCTGCTGGTC CCCGAGGTCC CCCTGGCTCT
GCTGGTGCTC CTGGCAAAGA TGGACTCAAC GGTCTCCCTG GCCCCATTGG GCCCCCTGGT
CCTCGCGGTC GCACTGGTGA TGCTGGTCCT GTTGGTCCCC CCGGCCCTCC TGGACCTCCT
GGTCCCCCTG GTCCTCCCAG CGCTGGTTTC GACTTCAGCT TCCTGCCCCA GCCACCTCAA
GAGAAGGCTC ACGATGGTGG CCGCTACTAC CGGGCTGATG ATGCCAATGT GGTTCGTGAC
CGTGACCTCG AGGTGGACAC CACCCTCAAG AGCCTGAGCC AGCAGATCGA GAACATCCGG
AGCCCAGAGG GCAGCCGCAA GAACCCCGCC CGCACCTGCC GTGACCTCAA GATGTGCCAC
TCTGACTGGA AGAGTGGAGA GTACTGGATT GACCCCAACC AAGGCTGCAA CCTGGATGCC
ATCAAAGTCT TCTGCAACAT GGAGACTGGT GAGACCTGCG TGTACCCCAC TCAGCCCAGT
GTGGCCCAGA AGAACTGGTA CATCAGCAAG AACCCCAAGG ACAAGAGGCA TGTCTGGTTC
GGCGAGAGCA TGACCGATGG ATTCCAGTTC GAGTATGGCG GCCAGGGCTC CGACCCTGCC
GATGTGGCCA TCCAGCTGAC CTTCCTGCGC CTGATGTCCA CCGAGGCCTC CCAGAACATC
ACCTACCACT GCAAGAACAG CGTGGCCTAC ATGGACCAGC AGACTGGCAA CCTCAAGAAG
GCCCTGCTCC TCCAGGGCTC CAACGAGATC GAGATCCGCG CCGAGGGCAA CAGCCGCTTC
ACCTACAGCG TCACTGTCGA TGGCTGCACG AGTCACACCG GAGCCTGGGG CAAGACAGTG
ATTGAATACA AAACCACCAA GACCTCCCGC CTGCCCATCA TCGATGTGGC CCCCTTGGAC
GTTGGTGCCC CAGACCAGGA ATTCGGCTTC GACGTTGGCC CTGTCTGCTT CCTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011522643.1
CDS136..4332
Misc Feature(1)253..420
Misc Feature(2)841..1020
Misc Feature(3)967..1146
Misc Feature(4)1057..1299
Misc Feature(5)1291..1542
Misc Feature(6)1462..1623
Misc Feature(7)1543..1758
Misc Feature(8)1939..2112
Misc Feature(9)2038..2214
Misc Feature(10)3625..4326
Translation

Target ORF information:

RefSeq Version XM_011524341.1
Organism Homo sapiens(Human)
Definition Homo sapiens collagen type I alpha 1 (COL1A1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524341.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
ATGTTCAGCT TTGTGGACCT CCGGCTCCTG CTCCTCTTAG CGGCCACCGC CCTCCTGACG 
CACGGCCAAG AGGAAGGCCA AGTCGAGGGC CAAGACGAAG ACATCCCACC AATCACCTGC
GTACAGAACG GCCTCAGGTA CCATGACCGA GACGTGTGGA AACCCGAGCC CTGCCGGATC
TGCGTCTGCG ACAACGGCAA GGTGTTGTGC GATGACGTGA TCTGTGACGA GACCAAGAAC
TGCCCCGGCG CCGAAGTCCC CGAGGGCGAG TGCTGTCCCG TCTGCCCCGA CGGCTCAGAG
TCACCCACCG ACCAAGAAAC CACCGGCGTC GAGGGACCCA AGGGAGACAC TGGCCCCCGA
GGCCCAAGGG GACCCGCAGG CCCCCCTGGC CGAGATGGCA TCCCTGGACA GCCTGGACTT
CCCGGACCCC CCGGACCCCC CGGACCTCCC GGACCCCCTG GCCTCGGAGG AAACTTTGCT
CCCCAGCTGT CTTATGGCTA TGATGAGAAA TCAACCGGAG GAATTTCCGT GCCTGGCCCC
ATGGGTCCCT CTGGTCCTCG TGGTCTCCCT GGCCCCCCTG GTGCACCTGG TCCCCAAGGC
TTCCAAGGTC CCCCTGGTGA GCCTGGCGAG CCTGGAGCTT CAGGTCCCAT GGGTCCCCGA
GGTCCCCCAG GTCCCCCTGG AAAGAATGGA GATGATGGGG AAGCTGGAAA ACCTGGTCGT
CCTGGTGAGC GTGGGCCTCC TGGGCCTCAG GGTGCTCGAG GATTGCCCGG AACAGCTGGC
CTCCCTGGAA TGAAGGGACA CAGAGGTTTC AGTGGTTTGG ATGGTGCCAA GGGAGATGCT
GGTCCTGCTG GTCCTAAGGG TGAGCCTGGC AGCCCTGGTG AAAATGGAGC TCCTGGTCAG
ATGGGCCCCC GTGGCCTGCC TGGTGAGAGA GGTCGCCCTG GAGCCCCTGG CCCTGCTGGA
AACCCTGGTG CTGATGGACA GCCTGGTGCT AAAGGTGCCA ATGGTGCTCC TGGTATTGCT
GGTGCTCCTG GCTTCCCTGG TGCCCGAGGC CCCTCTGGAC CCCAGGGCCC CGGCGGCCCT
CCTGGTCCCA AGGGTAACAG CGGTGAACCT GGTGCTCCTG GCAGCAAAGG AGACACTGGT
GCTAAGGGAG AGCCTGGCCC TGTTGGTGTT CAAGGACCCC CTGGCCCTGC TGGAGAGGAA
GGAAAGCGAG GAGCTCGAGG TGAACCCGGA CCCACTGGCC TGCCCGGACC CCCTGGCGAG
CGTGGTGGAC CTGGTAGCCG TGGTTTCCCT GGCGCAGATG GTGTTGCTGG TCCCAAGGGT
CCCGCTGGTG AACGTGGTTC TCCTGGCCCT GCTGGCCCCA AAGGATCTCC TGGTGAAGCT
GGTCGTCCCG GTGAAGCTGG TCTGCCTGGT GCCAAGGGTC TGACTGGAAG CCCTGGCAGC
CCTGGTCCTG ATGGCAAAAC TGGCCCCCCT GGTCCCGCCG GTCAAGATGG TCGCCCCGGA
CCCCCAGGCC CACCTGGTGC CCGTGGTCAG GCTGGTGTGA TGGGATTCCC TGGACCTAAA
GGTGCTGCTG GAGAGCCCGG CAAGGCTGGA GAGCGAGGTG TTCCCGGACC CCCTGGCGCT
GTCGGTCCTG CTGGCAAAGA TGGAGAGGCT GGAGCTCAGG GACCCCCTGG CCCTGCTGGT
CCCGCTGGCG AGAGAGGTGA ACAAGGCCCT GCTGGCTCCC CCGGATTCCA GGGTCTCCCT
GGTCCTGCTG GTCCTCCAGG TGAAGCAGGC AAACCTGGTG AACAGGGTGT TCCTGGAGAC
CTTGGCGCCC CTGGCCCCTC TGGAGCAAGA GGCGAGAGAG GTTTCCCTGG CGAGCGTGGT
GTGCAAGGTC CCCCTGGTCC TGCTGGTCCC CGAGGGGCCA ACGGTGCTCC CGGCAACGAT
GGTGCTAAGG GTGATGCTGG TGCCCCTGGA GCTCCCGGTA GCCAGGGCGC CCCTGGCCTT
CAGGGAATGC CTGGTGAACG TGGTGCAGCT GGTCTTCCAG GGCCTAAGGG TGACAGAGGT
GATGCTGGTC CCAAAGGTGC TGATGGCTCT CCTGGCAAAG ATGGCGTCCG TGGTCTGACT
GGCCCCATTG GTCCTCCTGG CCCTGCTGGT GCCCCTGGTG ACAAGGGTGA AAGTGGTCCC
AGCGGCCCTG CTGGTCCCAC TGGAGCTCGT GGTGCCCCCG GAGACCGTGG TGAGCCTGGT
CCCCCCGGCC CTGCTGGCTT TGCTGGCCCC CCTGGTGCTG ACGGCCAACC TGGTGCTAAA
GGCGAACCTG GTGATGCTGG TGCTAAAGGC GATGCTGGTC CCCCTGGCCC TGCCGGACCC
GCTGGACCCC CTGGCCCCAT TGGTAATGTT GGTGCTCCTG GAGCCAAAGG TGCTCGCGGC
AGCGCTGGTC CCCCTGGTGC TACTGGTTTC CCTGGTGCTG CTGGCCGAGT CGGTCCTCCT
GGCCCCTCTG GAAATGCTGG ACCCCCTGGC CCTCCTGGTC CTGCTGGCAA AGAAGGCGGC
AAAGGTCCCC GTGGTGAGAC TGGCCCTGCT GGACGTCCTG GTGAAGTTGG TCCCCCTGGT
CCCCCTGGCC CTGCTGGCGA GAAAGGATCC CCTGGTGCTG ATGGTCCTGC TGGTGCTCCT
GGTACTCCCG GGCCTCAAGG TATTGCTGGA CAGCGTGGTG TGGTCGGCCT GCCTGGTCAG
AGAGGAGAGA GAGGCTTCCC TGGTCTTCCT GGCCCCTCTG GTGAACCTGG CAAACAAGGT
CCCTCTGGAG CAAGTGGTGA ACGTGGTCCC CCTGGTCCCA TGGGCCCCCC TGGATTGGCT
GGACCCCCTG GTGAATCTGG ACGTGAGGGG GCTCCTGGTG CCGAAGGTTC CCCTGGACGA
GACGGTTCTC CTGGCGCCAA GGGTGACCGT GGTGAGACCG GCCCCGCTGG ACCCCCTGGT
GCTCCTGGTG CTCCTGGTGC CCCTGGCCCC GTTGGCCCTG CTGGCAAGAG TGGTGATCGT
GGTGAGACTG GTCCTGCTGG TCCCGCCGGT CCTGTCGGCC CTGTTGGCGC CCGTGGCCCC
GCCGGACCCC AAGGCCCCCG TGGTGACAAG GGTGAGACAG GCGAACAGGG CGACAGAGGC
ATAAAGGGTC ACCGTGGCTT CTCTGGCCTC CAGGGTCCCC CTGGCCCTCC TGGCTCTCCT
GGTGAACAAG GTCCCTCTGG AGCCTCTGGT CCTGCTGGTC CCCGAGGTCC CCCTGGCTCT
GCTGGTGCTC CTGGCAAAGA TGGACTCAAC GGTCTCCCTG GCCCCATTGG GCCCCCTGGT
CCTCGCGGTC GCACTGGTGA TGCTGGTCCT GTTGGTCCCC CCGGCCCTCC TGGACCTCCT
GGTCCCCCTG GTCCTCCCAG CGCTGGTTTC GACTTCAGCT TCCTGCCCCA GCCACCTCAA
GAGAAGGCTC ACGATGGTGG CCGCTACTAC CGGGCTGATG ATGCCAATGT GGTTCGTGAC
CGTGACCTCG AGGTGGACAC CACCCTCAAG AGCCTGAGCC AGCAGATCGA GAACATCCGG
AGCCCAGAGG GCAGCCGCAA GAACCCCGCC CGCACCTGCC GTGACCTCAA GATGTGCCAC
TCTGACTGGA AGAGTGGAGA GTACTGGATT GACCCCAACC AAGGCTGCAA CCTGGATGCC
ATCAAAGTCT TCTGCAACAT GGAGACTGGT GAGACCTGCG TGTACCCCAC TCAGCCCAGT
GTGGCCCAGA AGAACTGGTA CATCAGCAAG AACCCCAAGG ACAAGAGGCA TGTCTGGTTC
GGCGAGAGCA TGACCGATGG ATTCCAGTTC GAGTATGGCG GCCAGGGCTC CGACCCTGCC
GATGTGGCCA TCCAGCTGAC CTTCCTGCGC CTGATGTCCA CCGAGGCCTC CCAGAACATC
ACCTACCACT GCAAGAACAG CGTGGCCTAC ATGGACCAGC AGACTGGCAA CCTCAAGAAG
GCCCTGCTCC TCCAGGGCTC CAACGAGATC GAGATCCGCG CCGAGGGCAA CAGCCGCTTC
ACCTACAGCG TCACTGTCGA TGGCTGCACG AGTCACACCG GAGCCTGGGG CAAGACAGTG
ATTGAATACA AAACCACCAA GACCTCCCGC CTGCCCATCA TCGATGTGGC CCCCTTGGAC
GTTGGTGCCC CAGACCAGGA ATTCGGCTTC GACGTTGGCC CTGTCTGCTT CCTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Effects of GABA on the expression of type I collagen gene in normal human dermal fibroblasts.
Bioscience, biotechnology, and biochemistry81(2)376-379(2017 Feb)
Uehara E,Hokazono H,Sasaki T,Yoshioka H,Matsuo N


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Collagen analysis of the ascending aortic dilatation associated with bicuspid aortic valve disease compared with tricuspid aortic valve.
Archives of physiology and biochemistry122(5)289-294(2016 Dec)
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Identification of COL1A1 and COL1A2 as candidate prognostic factors in gastric cancer.
World journal of surgical oncology14(1)297(2016 Nov)
Li J,Ding Y,Li A


book

Clinical characteristics and the identification of novel mutations of COL1A1 and COL1A2 in 61 Chinese patients with osteogenesis imperfecta.
Molecular medicine reports14(5)4918-4926(2016 Nov)
Zhang H,Yue H,Wang C,Hu W,Gu J,He J,Fu W,Hu Y,Li M,Zhang Z


 
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