COL4A4 cDNA ORF clone, Homo sapiens(human)

The following COL4A4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL4A4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu19492 NM_000092.5
Latest version!
Homo sapiens collagen type IV alpha 4 chain (COL4A4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu19492 XM_005246281.3
Latest version!
Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu35833 XM_006712246.3
Latest version!
Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
OHu35834 XM_011510559.2
Latest version!
Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
OHu35834 XM_011510560.2
Latest version!
Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
OHu35836 XM_017003298.1
Latest version!
Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
OHu35836 XM_011510561.2
Latest version!
Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
OHu35836 XM_011510562.2
Latest version!
Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
OHu35837 XM_005246282.3
Latest version!
Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
OHu35839 XM_011510567.2
Latest version!
Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OHu35839 XM_011510566.2
Latest version!
Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OHu56971 XM_011510557.2
Latest version!
Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
OHu56972 XM_011510558.2
Latest version!
Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
OHu56975 XM_011510565.2
Latest version!
Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OHu56976 XM_011510568.2
Latest version!
Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OHu56977 XM_017003300.1
Latest version!
Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OHu56977 XM_011510570.2
Latest version!
Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OHu56977 XM_011510569.2
Latest version!
Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OHu56978 XM_011510572.3
Latest version!
Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OHu88997 XM_017003296.1
Latest version!
Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
OHu88998 XM_017003297.1
Latest version!
Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
OHu88999 XM_017003299.1
Latest version!
Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
View Old Accession Versions >>
OHu19492 NM_000092.4 Homo sapiens collagen type IV alpha 4 chain (COL4A4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu56978 XM_011510572.2 Homo sapiens collagen type IV alpha 4 (COL4A4), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OHu19492 XM_005246281.2 Homo sapiens collagen, type IV, alpha 4 (COL4A4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu19492
    Clone ID Related Accession (Same CDS sequence) NM_000092.5 , NM_000092.4 , XM_005246281.2 , XM_005246281.3
    Accession Version NM_000092.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5073bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product collagen alpha-4(IV) chain precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC079235.7, X81053.1, BX094848.1 and AC073149.5. On Aug 31, 2019 this sequence version replaced NM_000092.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X81053.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT
    GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC
    CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT
    GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC
    CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG
    AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG
    CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC
    TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC
    CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC
    CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA
    TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC
    AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT
    TGCCACTTTT TCGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG
    TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC
    AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000083.3
    CDS301..5373
    Translation

    Target ORF information:

    RefSeq Version NM_000092.5
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 4 chain (COL4A4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000092.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT
    GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC
    CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT
    GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC
    CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG
    AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG
    CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC
    TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC
    CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC
    CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA
    TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC
    AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT
    TGCCACTTTT TCGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG
    TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC
    AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19492
    Clone ID Related Accession (Same CDS sequence) NM_000092.5 , NM_000092.4 , XM_005246281.2 , XM_005246281.3
    Accession Version NM_000092.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5073bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-06
    Organism Homo sapiens(human)
    Product collagen alpha-4(IV) chain precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC079235.7, X81053.1, BX094848.1 and AC073149.5. This sequence is a reference standard in the RefSeqGene project. On Oct 17, 2006 this sequence version replaced NM_000092.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X81053.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT
    GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC
    CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT
    GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC
    CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG
    AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG
    CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC
    TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC
    CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC
    CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA
    TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC
    AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT
    TGCCACTTTT TCGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG
    TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC
    AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000083.3
    CDS655..5727
    Translation

    Target ORF information:

    RefSeq Version NM_000092.4
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 4 chain (COL4A4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000092.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT
    GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC
    CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT
    GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC
    CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG
    AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG
    CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC
    TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC
    CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC
    CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA
    TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC
    AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT
    TGCCACTTTT TCGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG
    TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC
    AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19492
    Clone ID Related Accession (Same CDS sequence) NM_000092.5 , NM_000092.4 , XM_005246281.2 , XM_005246281.3
    Accession Version XM_005246281.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5073bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product collagen alpha-4(IV) chain isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT
    GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC
    CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT
    GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC
    CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG
    AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG
    CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC
    TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC
    CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC
    CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA
    TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC
    AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT
    TGCCACTTTT TCGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG
    TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC
    AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005246338.1
    Translation

    Target ORF information:

    RefSeq Version XM_005246281.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens collagen, type IV, alpha 4 (COL4A4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005246281.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT
    GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC
    CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT
    GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC
    CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG
    AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG
    CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC
    TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC
    CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC
    CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA
    TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC
    AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT
    TGCCACTTTT TCGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG
    TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC
    AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19492
    Clone ID Related Accession (Same CDS sequence) NM_000092.5 , NM_000092.4 , XM_005246281.2 , XM_005246281.3
    Accession Version XM_005246281.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5073bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-4(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005246281.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT
    GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC
    CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT
    GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC
    CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG
    AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG
    CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC
    TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC
    CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC
    CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA
    TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC
    AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT
    TGCCACTTTT TCGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG
    TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC
    AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005246338.1
    CDS189..5261
    Translation

    Target ORF information:

    RefSeq Version XM_005246281.3
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005246281.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT
    GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC
    CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT
    GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC
    CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG
    AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG
    CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC
    TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC
    CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC
    CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA
    TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC
    AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT
    TGCCACTTTT TCGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG
    TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC
    AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu35833
    Clone ID Related Accession (Same CDS sequence) XM_006712246.3
    Accession Version XM_006712246.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4884bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-4(IV) chain isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712246.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT
    GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC
    CCTGGAGGAC CAGGTCTGGC AGGGTCTTGC CTTCCCGTAT TTAGCACGCT GCCCTTTGCC
    TACTGCAACA TCCACCAGGT GTGCCACTAT GCCCAGAGAA ACGACAGATC CTACTGGCTG
    GCCAGCGCTG CGCCCCTCCC CATGATGCCA CTCTCTGAAG AGGCGATCCG CCCCTATGTC
    AGCCGCTGTG CGGTATGCGA GGCCCCGGCC CAGGCGGTGG CGGTGCACAG CCAGGACCAG
    TCCATCCCCC CATGTCCGCA GACCTGGAGG AGCCTCTGGA TCGGGTATTC ATTCCTGATG
    CACACAGGAG CTGGGGACCA AGGAGGAGGG CAGGCCCTTA TGTCACCTGG CAGCTGCCTG
    GAAGATTTCA GAGCAGCACC ATTCCTTGAA TGCCAGGGCC GGCAGGGAAC TTGCCACTTT
    TTCGCAAATA AGTATAGCTT CTGGCTCACA ACGGTGAAAG CAGACTTGCA GTTTTCCTCT
    GCTCCAGCAC CAGACACCTT AAAAGAAAGC CAGGCCCAAC GCCAGAAAAT CAGCCGGTGC
    CAGGTCTGCG TGAAGTATAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006712309.1
    CDS327..5210
    Translation

    Target ORF information:

    RefSeq Version XM_006712246.3
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006712246.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT
    GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC
    CCTGGAGGAC CAGGTCTGGC AGGGTCTTGC CTTCCCGTAT TTAGCACGCT GCCCTTTGCC
    TACTGCAACA TCCACCAGGT GTGCCACTAT GCCCAGAGAA ACGACAGATC CTACTGGCTG
    GCCAGCGCTG CGCCCCTCCC CATGATGCCA CTCTCTGAAG AGGCGATCCG CCCCTATGTC
    AGCCGCTGTG CGGTATGCGA GGCCCCGGCC CAGGCGGTGG CGGTGCACAG CCAGGACCAG
    TCCATCCCCC CATGTCCGCA GACCTGGAGG AGCCTCTGGA TCGGGTATTC ATTCCTGATG
    CACACAGGAG CTGGGGACCA AGGAGGAGGG CAGGCCCTTA TGTCACCTGG CAGCTGCCTG
    GAAGATTTCA GAGCAGCACC ATTCCTTGAA TGCCAGGGCC GGCAGGGAAC TTGCCACTTT
    TTCGCAAATA AGTATAGCTT CTGGCTCACA ACGGTGAAAG CAGACTTGCA GTTTTCCTCT
    GCTCCAGCAC CAGACACCTT AAAAGAAAGC CAGGCCCAAC GCCAGAAAAT CAGCCGGTGC
    CAGGTCTGCG TGAAGTATAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu35834
    Clone ID Related Accession (Same CDS sequence) XM_011510560.2 , XM_011510559.2
    Accession Version XM_011510560.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-4(IV) chain isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510560.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT
    GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC
    CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT
    GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC
    CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG
    AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG
    CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC
    TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC
    CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC
    CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA
    TTCCTGATGG TGGAACTAGA GGAAGACAAA CTGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508862.1
    CDS327..5165
    Translation

    Target ORF information:

    RefSeq Version XM_011510560.2
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510560.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT
    GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC
    CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT
    GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC
    CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG
    AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG
    CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC
    TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC
    CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC
    CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA
    TTCCTGATGG TGGAACTAGA GGAAGACAAA CTGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu35834
    Clone ID Related Accession (Same CDS sequence) XM_011510560.2 , XM_011510559.2
    Accession Version XM_011510559.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-4(IV) chain isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510559.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT
    GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC
    CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT
    GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC
    CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG
    AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG
    CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC
    TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC
    CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC
    CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA
    TTCCTGATGG TGGAACTAGA GGAAGACAAA CTGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508861.1
    CDS327..5165
    Translation

    Target ORF information:

    RefSeq Version XM_011510559.2
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510559.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT
    GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC
    CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT
    GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC
    CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG
    AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG
    CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC
    TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC
    CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC
    CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA
    TTCCTGATGG TGGAACTAGA GGAAGACAAA CTGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu35836
    Clone ID Related Accession (Same CDS sequence) XM_017003298.1 , XM_011510562.2 , XM_011510561.2
    Accession Version XM_017003298.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-4(IV) chain isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT
    GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC
    CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT
    GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC
    CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG
    AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG
    CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC
    TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC
    CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC
    CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA
    TTCCTGATGC TCATGGGTGT GTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016858787.1
    CDS327..5153
    Translation

    Target ORF information:

    RefSeq Version XM_017003298.1
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017003298.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT
    GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC
    CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT
    GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC
    CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG
    AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG
    CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC
    TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC
    CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC
    CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA
    TTCCTGATGC TCATGGGTGT GTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu35836
    Clone ID Related Accession (Same CDS sequence) XM_017003298.1 , XM_011510562.2 , XM_011510561.2
    Accession Version XM_011510562.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-4(IV) chain isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510562.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT
    GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC
    CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT
    GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC
    CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG
    AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG
    CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC
    TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC
    CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC
    CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA
    TTCCTGATGC TCATGGGTGT GTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508864.1
    CDS327..5153
    Translation

    Target ORF information:

    RefSeq Version XM_011510562.2
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510562.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT
    GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC
    CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT
    GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC
    CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG
    AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG
    CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC
    TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC
    CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC
    CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA
    TTCCTGATGC TCATGGGTGT GTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu35836
    Clone ID Related Accession (Same CDS sequence) XM_017003298.1 , XM_011510562.2 , XM_011510561.2
    Accession Version XM_011510561.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-4(IV) chain isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510561.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT
    GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC
    CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT
    GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC
    CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG
    AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG
    CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC
    TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC
    CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC
    CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA
    TTCCTGATGC TCATGGGTGT GTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508863.1
    CDS327..5153
    Translation

    Target ORF information:

    RefSeq Version XM_011510561.2
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510561.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT
    GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC
    CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT
    GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC
    CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG
    AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG
    CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC
    TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC
    CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC
    CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA
    TTCCTGATGC TCATGGGTGT GTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu35837
    Clone ID Related Accession (Same CDS sequence) XM_005246282.3
    Accession Version XM_005246282.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4518bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-4(IV) chain isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005246282.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGAAATG AAGGACTCTG TGCCTGTGAG CCTGGACCCA TGGGCCCCCC TGGCCCTCCA
    GGACTTCCTG GGAGGCAGGG GAGTAAGGGA GACTTGGGGC TCCCTGGCTG GCTTGGAACA
    AAAGGTGACC CAGGACCTCC TGGTGCTGAA GGACCTCCAG GGCTACCAGG AAAGCATGGT
    GCCTCTGGAC CACCTGGCAA CAAAGGGGCG AAGGGTGACA TGGTTGTATC AAGAGTTAAA
    GGGCACAAAG GAGAAAGAGG TCCTGATGGG CCCCCAGGAT TTCCAGGGCA GCCAGGATCA
    CATGGTCGGG ATGGACATGC TGGAGAAAAA GGGGATCCAG GACCTCCAGG GGATCATGAA
    GATGCGACCC CAGGTGGTAA AGGATTTCCT GGACCTCTGG GCCCCCCAGG CAAAGCAGGA
    CCTGTGGGGC CCCCAGGACT GGGATTTCCT GGTCCACCAG GAGAGCGAGG CCACCCAGGA
    GTTCCAGGCC ACCCAGGTGT GAGGGGCCCT GATGGCTTGA AGGGTCAGAA AGGTGACACA
    ATTTCTTGCA ACGTAACCTA CCCTGGGAGG CATGGCCCTC CAGGTTTTGA TGGACCTCCA
    GGTCCGAAGG GATTTCCAGG TCCCCAAGGT GCCCCTGGGC TGAGTGGTTC AGATGGGCAT
    AAAGGCAGAC CTGGCACACC AGGAACAGCG GAAATACCAG GTCCACCTGG TTTTCGTGGT
    GACATGGGAG ATCCGGGTTT TGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGC
    CCTCCCGGCT CACCAGGAGT GAATGGTCAG AAAGGAATCC CGGGAGACCC TGCATTTGGT
    CACCTGGGAC CCCCGGGAAA GAGGGGTCTT TCAGGAGTGC CAGGGATAAA AGGACCCAGA
    GGTGATCCGG GATGTCCAGG GGCTGAAGGG CCAGCTGGCA TTCCTGGATT CCTAGGTCTC
    AAAGGTCCCA AAGGCAGAGA GGGACATGCT GGGTTTCCAG GTGTCCCAGG TCCACCTGGC
    CATTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGACTCCC TGGGTATCCA
    GGTAGCCCAG GTGCTCCAGG TGGGAAAGGA CAGCCGGGAG ATGTGGGGCC TCCCGGGCCA
    GCTGGAATGA AAGGCCTCCC CGGACTCCCA GGACGGCCTG GGGCACATGG TCCCCCAGGC
    CTCCCAGGAA TCCCAGGTCC CTTTGGAGAT GATGGGCTAC CTGGTCCTCC AGGTCCAAAG
    GGACCCCGGG GGCTGCCTGG TTTCCCAGGT TTTCCCGGAG AAAGAGGAAA GCCTGGTGCA
    GAGGGATGTC CTGGCGCAAA GGGAGAACCT GGAGAGAAGG GCATGTCTGG CCTTCCTGGA
    GACCGGGGAC TGAGAGGGGC CAAAGGAGCC ATAGGACCTC CCGGAGATGA AGGAGAAATG
    GCTATCATTT CACAAAAGGG AACACCTGGG GAACCTGGAC CTCCTGGAGA TGATGGATTC
    CCAGGAGAAA GAGGTGATAA AGGAACTCCC GGGATGCAAG GGAGAAGAGG AGAGCCGGGA
    AGATACGGAC CACCTGGATT TCACAGAGGG GAACCTGGTG AGAAAGGTCA GCCAGGGCCT
    CCTGGACCCC CAGGCCCTCC AGGCTCAACT GGTCTAAGAG GGTTCATTGG TTTTCCAGGA
    CTTCCAGGTG ACCAGGGTGA GCCAGGTTCT CCAGGTCCCC CTGGATTTTC AGGAATTGAT
    GGAGCAAGAG GACCTAAAGG AAACAAAGGT GACCCTGCCA GTCACTTTGG TCCACCTGGT
    CCAAAGGGTG AGCCAGGTAG CCCTGGATGT CCAGGGCATT TTGGAGCATC CGGAGAGCAG
    GGCTTGCCTG GTATTCAAGG GCCCAGAGGA TCACCTGGAA GGCCAGGGCC ACCTGGCTCC
    TCTGGACCAC CAGGGTGCCC AGGTGATCAC GGGATGCCTG GGCTGAGGGG ACAGCCAGGA
    GAAATGGGAG ACCCTGGGCC AAGAGGCCTC CAGGGGGATC CAGGGATACC AGGTCCTCCG
    GGAATAAAAG GTCCCTCCGG ATCACCTGGC CTGAACGGCT TGCATGGATT GAAAGGTCAG
    AAAGGAACTA AAGGTGCTTC AGGTTTGCAT GATGTGGGGC CACCTGGTCC AGTGGGAATA
    CCTGGGCTAA AAGGGGAGAG AGGAGACCCT GGGAGCCCAG GAATCTCTCC TCCAGGTCCT
    CGTGGAAAGA AAGGTCCCCC AGGACCCCCA GGGAGTTCAG GACCACCTGG TCCTGCAGGT
    GCCACAGGAA GAGCTCCTAA GGACATTCCT GACCCGGGTC CACCTGGAGA TCAGGGACCT
    CCTGGTCCTG ATGGCCCAAG AGGAGCACCT GGGCCTCCAG GCCTCCCTGG GAGTGTTGAC
    CTTCTGAGAG GGGAGCCAGG TGACTGTGGT CTACCAGGGC CACCAGGTCC CCCTGGCCCA
    CCAGGCCCTC CAGGATACAA AGGCTTTCCA GGATGTGATG GAAAAGATGG CCAGAAAGGA
    CCAGTGGGAT TCCCGGGACC GCAGGGACCA CATGGATTTC CTGGGCCACC TGGAGAGAAG
    GGTTTACCTG GACCTCCAGG GAGAAAAGGG CCCACTGGTC TTCCGGGTCC CAGAGGTGAA
    CCGGGGCCAC CTGCAGATGT GGATGACTGT CCCCGAATCC CAGGCCTTCC TGGGGCGCCA
    GGCATGAGAG GACCAGAAGG AGCCATGGGG CTCCCTGGAA TGAGAGGCCC CTCAGGACCA
    GGGTGCAAAG GAGAGCCTGG GCTGGATGGC AGGAGGGGTG TGGATGGCGT CCCTGGGTCT
    CCTGGGCCTC CCGGACGTAA AGGTGACACA GGAGAAGACG GCTACCCTGG AGGACCAGGG
    CCTCCTGGTC CCATTGGGGA TCCTGGGCCC AAAGGGTTTG GCCCTGGATA CCTCGGTGGC
    TTCCTCCTGG TTCTCCACAG TCAGACGGAC CAGGAGCCCA CCTGCCCCCT GGGCATGCCC
    AGGCTCTGGA CTGGGTATAG TCTGTTATAC CTGGAAGGGC AAGAGAAAGC TCACAATCAA
    GACCTTGGTC TGGCAGGGTC TTGCCTTCCC GTATTTAGCA CGCTGCCCTT TGCCTACTGC
    AACATCCACC AGGTGTGCCA CTATGCCCAG AGAAACGACA GATCCTACTG GCTGGCCAGC
    GCTGCGCCCC TCCCCATGAT GCCACTCTCT GAAGAGGCGA TCCGCCCCTA TGTCAGCCGC
    TGTGCGGTAT GCGAGGCCCC GGCCCAGGCG GTGGCGGTGC ACAGCCAGGA CCAGTCCATC
    CCCCCATGTC CGCAGACCTG GAGGAGCCTC TGGATCGGGT ATTCATTCCT GATGCACACA
    GGAGCTGGGG ACCAAGGAGG AGGGCAGGCC CTTATGTCAC CTGGCAGCTG CCTGGAAGAT
    TTCAGAGCAG CACCATTCCT TGAATGCCAG GGCCGGCAGG GAACTTGCCA CTTTTTCGCA
    AATAAGTATA GCTTCTGGCT CACAACGGTG AAAGCAGACT TGCAGTTTTC CTCTGCTCCA
    GCACCAGACA CCTTAAAAGA AAGCCAGGCC CAACGCCAGA AAATCAGCCG GTGCCAGGTC
    TGCGTGAAGT ATAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005246339.1
    CDS327..4844
    Translation

    Target ORF information:

    RefSeq Version XM_005246282.3
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005246282.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGAAATG AAGGACTCTG TGCCTGTGAG CCTGGACCCA TGGGCCCCCC TGGCCCTCCA
    GGACTTCCTG GGAGGCAGGG GAGTAAGGGA GACTTGGGGC TCCCTGGCTG GCTTGGAACA
    AAAGGTGACC CAGGACCTCC TGGTGCTGAA GGACCTCCAG GGCTACCAGG AAAGCATGGT
    GCCTCTGGAC CACCTGGCAA CAAAGGGGCG AAGGGTGACA TGGTTGTATC AAGAGTTAAA
    GGGCACAAAG GAGAAAGAGG TCCTGATGGG CCCCCAGGAT TTCCAGGGCA GCCAGGATCA
    CATGGTCGGG ATGGACATGC TGGAGAAAAA GGGGATCCAG GACCTCCAGG GGATCATGAA
    GATGCGACCC CAGGTGGTAA AGGATTTCCT GGACCTCTGG GCCCCCCAGG CAAAGCAGGA
    CCTGTGGGGC CCCCAGGACT GGGATTTCCT GGTCCACCAG GAGAGCGAGG CCACCCAGGA
    GTTCCAGGCC ACCCAGGTGT GAGGGGCCCT GATGGCTTGA AGGGTCAGAA AGGTGACACA
    ATTTCTTGCA ACGTAACCTA CCCTGGGAGG CATGGCCCTC CAGGTTTTGA TGGACCTCCA
    GGTCCGAAGG GATTTCCAGG TCCCCAAGGT GCCCCTGGGC TGAGTGGTTC AGATGGGCAT
    AAAGGCAGAC CTGGCACACC AGGAACAGCG GAAATACCAG GTCCACCTGG TTTTCGTGGT
    GACATGGGAG ATCCGGGTTT TGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGC
    CCTCCCGGCT CACCAGGAGT GAATGGTCAG AAAGGAATCC CGGGAGACCC TGCATTTGGT
    CACCTGGGAC CCCCGGGAAA GAGGGGTCTT TCAGGAGTGC CAGGGATAAA AGGACCCAGA
    GGTGATCCGG GATGTCCAGG GGCTGAAGGG CCAGCTGGCA TTCCTGGATT CCTAGGTCTC
    AAAGGTCCCA AAGGCAGAGA GGGACATGCT GGGTTTCCAG GTGTCCCAGG TCCACCTGGC
    CATTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGACTCCC TGGGTATCCA
    GGTAGCCCAG GTGCTCCAGG TGGGAAAGGA CAGCCGGGAG ATGTGGGGCC TCCCGGGCCA
    GCTGGAATGA AAGGCCTCCC CGGACTCCCA GGACGGCCTG GGGCACATGG TCCCCCAGGC
    CTCCCAGGAA TCCCAGGTCC CTTTGGAGAT GATGGGCTAC CTGGTCCTCC AGGTCCAAAG
    GGACCCCGGG GGCTGCCTGG TTTCCCAGGT TTTCCCGGAG AAAGAGGAAA GCCTGGTGCA
    GAGGGATGTC CTGGCGCAAA GGGAGAACCT GGAGAGAAGG GCATGTCTGG CCTTCCTGGA
    GACCGGGGAC TGAGAGGGGC CAAAGGAGCC ATAGGACCTC CCGGAGATGA AGGAGAAATG
    GCTATCATTT CACAAAAGGG AACACCTGGG GAACCTGGAC CTCCTGGAGA TGATGGATTC
    CCAGGAGAAA GAGGTGATAA AGGAACTCCC GGGATGCAAG GGAGAAGAGG AGAGCCGGGA
    AGATACGGAC CACCTGGATT TCACAGAGGG GAACCTGGTG AGAAAGGTCA GCCAGGGCCT
    CCTGGACCCC CAGGCCCTCC AGGCTCAACT GGTCTAAGAG GGTTCATTGG TTTTCCAGGA
    CTTCCAGGTG ACCAGGGTGA GCCAGGTTCT CCAGGTCCCC CTGGATTTTC AGGAATTGAT
    GGAGCAAGAG GACCTAAAGG AAACAAAGGT GACCCTGCCA GTCACTTTGG TCCACCTGGT
    CCAAAGGGTG AGCCAGGTAG CCCTGGATGT CCAGGGCATT TTGGAGCATC CGGAGAGCAG
    GGCTTGCCTG GTATTCAAGG GCCCAGAGGA TCACCTGGAA GGCCAGGGCC ACCTGGCTCC
    TCTGGACCAC CAGGGTGCCC AGGTGATCAC GGGATGCCTG GGCTGAGGGG ACAGCCAGGA
    GAAATGGGAG ACCCTGGGCC AAGAGGCCTC CAGGGGGATC CAGGGATACC AGGTCCTCCG
    GGAATAAAAG GTCCCTCCGG ATCACCTGGC CTGAACGGCT TGCATGGATT GAAAGGTCAG
    AAAGGAACTA AAGGTGCTTC AGGTTTGCAT GATGTGGGGC CACCTGGTCC AGTGGGAATA
    CCTGGGCTAA AAGGGGAGAG AGGAGACCCT GGGAGCCCAG GAATCTCTCC TCCAGGTCCT
    CGTGGAAAGA AAGGTCCCCC AGGACCCCCA GGGAGTTCAG GACCACCTGG TCCTGCAGGT
    GCCACAGGAA GAGCTCCTAA GGACATTCCT GACCCGGGTC CACCTGGAGA TCAGGGACCT
    CCTGGTCCTG ATGGCCCAAG AGGAGCACCT GGGCCTCCAG GCCTCCCTGG GAGTGTTGAC
    CTTCTGAGAG GGGAGCCAGG TGACTGTGGT CTACCAGGGC CACCAGGTCC CCCTGGCCCA
    CCAGGCCCTC CAGGATACAA AGGCTTTCCA GGATGTGATG GAAAAGATGG CCAGAAAGGA
    CCAGTGGGAT TCCCGGGACC GCAGGGACCA CATGGATTTC CTGGGCCACC TGGAGAGAAG
    GGTTTACCTG GACCTCCAGG GAGAAAAGGG CCCACTGGTC TTCCGGGTCC CAGAGGTGAA
    CCGGGGCCAC CTGCAGATGT GGATGACTGT CCCCGAATCC CAGGCCTTCC TGGGGCGCCA
    GGCATGAGAG GACCAGAAGG AGCCATGGGG CTCCCTGGAA TGAGAGGCCC CTCAGGACCA
    GGGTGCAAAG GAGAGCCTGG GCTGGATGGC AGGAGGGGTG TGGATGGCGT CCCTGGGTCT
    CCTGGGCCTC CCGGACGTAA AGGTGACACA GGAGAAGACG GCTACCCTGG AGGACCAGGG
    CCTCCTGGTC CCATTGGGGA TCCTGGGCCC AAAGGGTTTG GCCCTGGATA CCTCGGTGGC
    TTCCTCCTGG TTCTCCACAG TCAGACGGAC CAGGAGCCCA CCTGCCCCCT GGGCATGCCC
    AGGCTCTGGA CTGGGTATAG TCTGTTATAC CTGGAAGGGC AAGAGAAAGC TCACAATCAA
    GACCTTGGTC TGGCAGGGTC TTGCCTTCCC GTATTTAGCA CGCTGCCCTT TGCCTACTGC
    AACATCCACC AGGTGTGCCA CTATGCCCAG AGAAACGACA GATCCTACTG GCTGGCCAGC
    GCTGCGCCCC TCCCCATGAT GCCACTCTCT GAAGAGGCGA TCCGCCCCTA TGTCAGCCGC
    TGTGCGGTAT GCGAGGCCCC GGCCCAGGCG GTGGCGGTGC ACAGCCAGGA CCAGTCCATC
    CCCCCATGTC CGCAGACCTG GAGGAGCCTC TGGATCGGGT ATTCATTCCT GATGCACACA
    GGAGCTGGGG ACCAAGGAGG AGGGCAGGCC CTTATGTCAC CTGGCAGCTG CCTGGAAGAT
    TTCAGAGCAG CACCATTCCT TGAATGCCAG GGCCGGCAGG GAACTTGCCA CTTTTTCGCA
    AATAAGTATA GCTTCTGGCT CACAACGGTG AAAGCAGACT TGCAGTTTTC CTCTGCTCCA
    GCACCAGACA CCTTAAAAGA AAGCCAGGCC CAACGCCAGA AAATCAGCCG GTGCCAGGTC
    TGCGTGAAGT ATAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu35839
    Clone ID Related Accession (Same CDS sequence) XM_011510566.2 , XM_011510567.2
    Accession Version XM_011510566.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4263bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-4(IV) chain isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510566.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGTGG AACTAGAGGA AGACAAACTG CTCTGAGAAA AACCTGCAAT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508868.1
    CDS327..4589
    Translation

    Target ORF information:

    RefSeq Version XM_011510566.2
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510566.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGTGG AACTAGAGGA AGACAAACTG CTCTGAGAAA AACCTGCAAT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu35839
    Clone ID Related Accession (Same CDS sequence) XM_011510566.2 , XM_011510567.2
    Accession Version XM_011510567.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4263bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-4(IV) chain isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510567.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGTGG AACTAGAGGA AGACAAACTG CTCTGAGAAA AACCTGCAAT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508869.1
    CDS327..4589
    Translation

    Target ORF information:

    RefSeq Version XM_011510567.2
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510567.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGTGG AACTAGAGGA AGACAAACTG CTCTGAGAAA AACCTGCAAT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56971
    Clone ID Related Accession (Same CDS sequence) XM_011510557.2
    Accession Version XM_011510557.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4992bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-4(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510557.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGACTTCCA
    GGTGACCAGG GTGAGCCAGG TTCTCCAGGT CCCCCTGGAT TTTCAGGAAT TGATGGAGCA
    AGAGGACCTA AAGGAAACAA AGGTGACCCT GCCAGTCACT TTGGTCCACC TGGTCCAAAG
    GGTGAGCCAG GTAGCCCTGG ATGTCCAGGG CATTTTGGAG CATCCGGAGA GCAGGGCTTG
    CCTGGTATTC AAGGGCCCAG AGGATCACCT GGAAGGCCAG GGCCACCTGG CTCCTCTGGA
    CCACCAGGGT GCCCAGGTGA TCACGGGATG CCTGGGCTGA GGGGACAGCC AGGAGAAATG
    GGAGACCCTG GGCCAAGAGG CCTCCAGGGG GATCCAGGGA TACCAGGTCC TCCGGGAATA
    AAAGGTCCCT CCGGATCACC TGGCCTGAAC GGCTTGCATG GATTGAAAGG TCAGAAAGGA
    ACTAAAGGTG CTTCAGGTTT GCATGATGTG GGGCCACCTG GTCCAGTGGG AATACCTGGG
    CTAAAAGGGG AGAGAGGAGA CCCTGGGAGC CCAGGAATCT CTCCTCCAGG TCCTCGTGGA
    AAGAAAGGTC CCCCAGGACC CCCAGGGAGT TCAGGACCAC CTGGTCCTGC AGGTGCCACA
    GGAAGAGCTC CTAAGGACAT TCCTGACCCG GGTCCACCTG GAGATCAGGG ACCTCCTGGT
    CCTGATGGCC CAAGAGGAGC ACCTGGGCCT CCAGGCCTCC CTGGGAGTGT TGACCTTCTG
    AGAGGGGAGC CAGGTGACTG TGGTCTACCA GGGCCACCAG GTCCCCCTGG CCCACCAGGC
    CCTCCAGGAT ACAAAGGCTT TCCAGGATGT GATGGAAAAG ATGGCCAGAA AGGACCAGTG
    GGATTCCCGG GACCGCAGGG ACCACATGGA TTTCCTGGGC CACCTGGAGA GAAGGGTTTA
    CCTGGACCTC CAGGGAGAAA AGGGCCCACT GGTCTTCCGG GTCCCAGAGG TGAACCGGGG
    CCACCTGCAG ATGTGGATGA CTGTCCCCGA ATCCCAGGCC TTCCTGGGGC GCCAGGCATG
    AGAGGACCAG AAGGAGCCAT GGGGCTCCCT GGAATGAGAG GCCCCTCAGG ACCAGGGTGC
    AAAGGAGAGC CTGGGCTGGA TGGCAGGAGG GGTGTGGATG GCGTCCCTGG GTCTCCTGGG
    CCTCCCGGAC GTAAAGGTGA CACAGGAGAA GACGGCTACC CTGGAGGACC AGGGCCTCCT
    GGTCCCATTG GGGATCCTGG GCCCAAAGGG TTTGGCCCTG GATACCTCGG TGGCTTCCTC
    CTGGTTCTCC ACAGTCAGAC GGACCAGGAG CCCACCTGCC CCCTGGGCAT GCCCAGGCTC
    TGGACTGGGT ATAGTCTGTT ATACCTGGAA GGGCAAGAGA AAGCTCACAA TCAAGACCTT
    GGTCTGGCAG GGTCTTGCCT TCCCGTATTT AGCACGCTGC CCTTTGCCTA CTGCAACATC
    CACCAGGTGT GCCACTATGC CCAGAGAAAC GACAGATCCT ACTGGCTGGC CAGCGCTGCG
    CCCCTCCCCA TGATGCCACT CTCTGAAGAG GCGATCCGCC CCTATGTCAG CCGCTGTGCG
    GTATGCGAGG CCCCGGCCCA GGCGGTGGCG GTGCACAGCC AGGACCAGTC CATCCCCCCA
    TGTCCGCAGA CCTGGAGGAG CCTCTGGATC GGGTATTCAT TCCTGATGCA CACAGGAGCT
    GGGGACCAAG GAGGAGGGCA GGCCCTTATG TCACCTGGCA GCTGCCTGGA AGATTTCAGA
    GCAGCACCAT TCCTTGAATG CCAGGGCCGG CAGGGAACTT GCCACTTTTT CGCAAATAAG
    TATAGCTTCT GGCTCACAAC GGTGAAAGCA GACTTGCAGT TTTCCTCTGC TCCAGCACCA
    GACACCTTAA AAGAAAGCCA GGCCCAACGC CAGAAAATCA GCCGGTGCCA GGTCTGCGTG
    AAGTATAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508859.1
    CDS327..5318
    Translation

    Target ORF information:

    RefSeq Version XM_011510557.2
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510557.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGACTTCCA
    GGTGACCAGG GTGAGCCAGG TTCTCCAGGT CCCCCTGGAT TTTCAGGAAT TGATGGAGCA
    AGAGGACCTA AAGGAAACAA AGGTGACCCT GCCAGTCACT TTGGTCCACC TGGTCCAAAG
    GGTGAGCCAG GTAGCCCTGG ATGTCCAGGG CATTTTGGAG CATCCGGAGA GCAGGGCTTG
    CCTGGTATTC AAGGGCCCAG AGGATCACCT GGAAGGCCAG GGCCACCTGG CTCCTCTGGA
    CCACCAGGGT GCCCAGGTGA TCACGGGATG CCTGGGCTGA GGGGACAGCC AGGAGAAATG
    GGAGACCCTG GGCCAAGAGG CCTCCAGGGG GATCCAGGGA TACCAGGTCC TCCGGGAATA
    AAAGGTCCCT CCGGATCACC TGGCCTGAAC GGCTTGCATG GATTGAAAGG TCAGAAAGGA
    ACTAAAGGTG CTTCAGGTTT GCATGATGTG GGGCCACCTG GTCCAGTGGG AATACCTGGG
    CTAAAAGGGG AGAGAGGAGA CCCTGGGAGC CCAGGAATCT CTCCTCCAGG TCCTCGTGGA
    AAGAAAGGTC CCCCAGGACC CCCAGGGAGT TCAGGACCAC CTGGTCCTGC AGGTGCCACA
    GGAAGAGCTC CTAAGGACAT TCCTGACCCG GGTCCACCTG GAGATCAGGG ACCTCCTGGT
    CCTGATGGCC CAAGAGGAGC ACCTGGGCCT CCAGGCCTCC CTGGGAGTGT TGACCTTCTG
    AGAGGGGAGC CAGGTGACTG TGGTCTACCA GGGCCACCAG GTCCCCCTGG CCCACCAGGC
    CCTCCAGGAT ACAAAGGCTT TCCAGGATGT GATGGAAAAG ATGGCCAGAA AGGACCAGTG
    GGATTCCCGG GACCGCAGGG ACCACATGGA TTTCCTGGGC CACCTGGAGA GAAGGGTTTA
    CCTGGACCTC CAGGGAGAAA AGGGCCCACT GGTCTTCCGG GTCCCAGAGG TGAACCGGGG
    CCACCTGCAG ATGTGGATGA CTGTCCCCGA ATCCCAGGCC TTCCTGGGGC GCCAGGCATG
    AGAGGACCAG AAGGAGCCAT GGGGCTCCCT GGAATGAGAG GCCCCTCAGG ACCAGGGTGC
    AAAGGAGAGC CTGGGCTGGA TGGCAGGAGG GGTGTGGATG GCGTCCCTGG GTCTCCTGGG
    CCTCCCGGAC GTAAAGGTGA CACAGGAGAA GACGGCTACC CTGGAGGACC AGGGCCTCCT
    GGTCCCATTG GGGATCCTGG GCCCAAAGGG TTTGGCCCTG GATACCTCGG TGGCTTCCTC
    CTGGTTCTCC ACAGTCAGAC GGACCAGGAG CCCACCTGCC CCCTGGGCAT GCCCAGGCTC
    TGGACTGGGT ATAGTCTGTT ATACCTGGAA GGGCAAGAGA AAGCTCACAA TCAAGACCTT
    GGTCTGGCAG GGTCTTGCCT TCCCGTATTT AGCACGCTGC CCTTTGCCTA CTGCAACATC
    CACCAGGTGT GCCACTATGC CCAGAGAAAC GACAGATCCT ACTGGCTGGC CAGCGCTGCG
    CCCCTCCCCA TGATGCCACT CTCTGAAGAG GCGATCCGCC CCTATGTCAG CCGCTGTGCG
    GTATGCGAGG CCCCGGCCCA GGCGGTGGCG GTGCACAGCC AGGACCAGTC CATCCCCCCA
    TGTCCGCAGA CCTGGAGGAG CCTCTGGATC GGGTATTCAT TCCTGATGCA CACAGGAGCT
    GGGGACCAAG GAGGAGGGCA GGCCCTTATG TCACCTGGCA GCTGCCTGGA AGATTTCAGA
    GCAGCACCAT TCCTTGAATG CCAGGGCCGG CAGGGAACTT GCCACTTTTT CGCAAATAAG
    TATAGCTTCT GGCTCACAAC GGTGAAAGCA GACTTGCAGT TTTCCTCTGC TCCAGCACCA
    GACACCTTAA AAGAAAGCCA GGCCCAACGC CAGAAAATCA GCCGGTGCCA GGTCTGCGTG
    AAGTATAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56972
    Clone ID Related Accession (Same CDS sequence) XM_011510558.2
    Accession Version XM_011510558.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4965bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-4(IV) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510558.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTTT TCGTGGTGAC ATGGGAGATC CGGGTTTTGG AGGTGAAAAG
    GGGTCCTCCC CTGTTGGGCC CCCAGGCCCT CCCGGCTCAC CAGGAGTGAA TGGTCAGAAA
    GGAATCCCGG GAGACCCTGC ATTTGGTCAC CTGGGACCCC CGGGAAAGAG GGGTCTTTCA
    GGAGTGCCAG GGATAAAAGG ACCCAGAGGT GATCCGGGAT GTCCAGGGGC TGAAGGGCCA
    GCTGGCATTC CTGGATTCCT AGGTCTCAAA GGTCCCAAAG GCAGAGAGGG ACATGCTGGG
    TTTCCAGGTG TCCCAGGTCC ACCTGGCCAT TCCTGTGAAA GAGGTGCTCC AGGGATACCA
    GGGCAACCGG GACTCCCTGG GTATCCAGGT AGCCCAGGTG CTCCAGGTGG GAAAGGACAG
    CCGGGAGATG TGGGGCCTCC CGGGCCAGCT GGAATGAAAG GCCTCCCCGG ACTCCCAGGA
    CGGCCTGGGG CACATGGTCC CCCAGGCCTC CCAGGAATCC CAGGTCCCTT TGGAGATGAT
    GGGCTACCTG GTCCTCCAGG TCCAAAGGGA CCCCGGGGGC TGCCTGGTTT CCCAGGTTTT
    CCCGGAGAAA GAGGAAAGCC TGGTGCAGAG GGATGTCCTG GCGCAAAGGG AGAACCTGGA
    GAGAAGGGCA TGTCTGGCCT TCCTGGAGAC CGGGGACTGA GAGGGGCCAA AGGAGCCATA
    GGACCTCCCG GAGATGAAGG AGAAATGGCT ATCATTTCAC AAAAGGGAAC ACCTGGGGAA
    CCTGGACCTC CTGGAGATGA TGGATTCCCA GGAGAAAGAG GTGATAAAGG AACTCCCGGG
    ATGCAAGGGA GAAGAGGAGA GCCGGGAAGA TACGGACCAC CTGGATTTCA CAGAGGGGAA
    CCTGGTGAGA AAGGTCAGCC AGGGCCTCCT GGACCCCCAG GCCCTCCAGG CTCAACTGGT
    CTAAGAGGGT TCATTGGTTT TCCAGGACTT CCAGGTGACC AGGGTGAGCC AGGTTCTCCA
    GGTCCCCCTG GATTTTCAGG AATTGATGGA GCAAGAGGAC CTAAAGGAAA CAAAGGTGAC
    CCTGCCAGTC ACTTTGGTCC ACCTGGTCCA AAGGGTGAGC CAGGTAGCCC TGGATGTCCA
    GGGCATTTTG GAGCATCCGG AGAGCAGGGC TTGCCTGGTA TTCAAGGGCC CAGAGGATCA
    CCTGGAAGGC CAGGGCCACC TGGCTCCTCT GGACCACCAG GGTGCCCAGG TGATCACGGG
    ATGCCTGGGC TGAGGGGACA GCCAGGAGAA ATGGGAGACC CTGGGCCAAG AGGCCTCCAG
    GGGGATCCAG GGATACCAGG TCCTCCGGGA ATAAAAGGTC CCTCCGGATC ACCTGGCCTG
    AACGGCTTGC ATGGATTGAA AGGTCAGAAA GGAACTAAAG GTGCTTCAGG TTTGCATGAT
    GTGGGGCCAC CTGGTCCAGT GGGAATACCT GGGCTAAAAG GGGAGAGAGG AGACCCTGGG
    AGCCCAGGAA TCTCTCCTCC AGGTCCTCGT GGAAAGAAAG GTCCCCCAGG ACCCCCAGGG
    AGTTCAGGAC CACCTGGTCC TGCAGGTGCC ACAGGAAGAG CTCCTAAGGA CATTCCTGAC
    CCGGGTCCAC CTGGAGATCA GGGACCTCCT GGTCCTGATG GCCCAAGAGG AGCACCTGGG
    CCTCCAGGCC TCCCTGGGAG TGTTGACCTT CTGAGAGGGG AGCCAGGTGA CTGTGGTCTA
    CCAGGGCCAC CAGGTCCCCC TGGCCCACCA GGCCCTCCAG GATACAAAGG CTTTCCAGGA
    TGTGATGGAA AAGATGGCCA GAAAGGACCA GTGGGATTCC CGGGACCGCA GGGACCACAT
    GGATTTCCTG GGCCACCTGG AGAGAAGGGT TTACCTGGAC CTCCAGGGAG AAAAGGGCCC
    ACTGGTCTTC CGGGTCCCAG AGGTGAACCG GGGCCACCTG CAGATGTGGA TGACTGTCCC
    CGAATCCCAG GCCTTCCTGG GGCGCCAGGC ATGAGAGGAC CAGAAGGAGC CATGGGGCTC
    CCTGGAATGA GAGGCCCCTC AGGACCAGGG TGCAAAGGAG AGCCTGGGCT GGATGGCAGG
    AGGGGTGTGG ATGGCGTCCC TGGGTCTCCT GGGCCTCCCG GACGTAAAGG TGACACAGGA
    GAAGACGGCT ACCCTGGAGG ACCAGGGCCT CCTGGTCCCA TTGGGGATCC TGGGCCCAAA
    GGGTTTGGCC CTGGATACCT CGGTGGCTTC CTCCTGGTTC TCCACAGTCA GACGGACCAG
    GAGCCCACCT GCCCCCTGGG CATGCCCAGG CTCTGGACTG GGTATAGTCT GTTATACCTG
    GAAGGGCAAG AGAAAGCTCA CAATCAAGAC CTTGGTCTGG CAGGGTCTTG CCTTCCCGTA
    TTTAGCACGC TGCCCTTTGC CTACTGCAAC ATCCACCAGG TGTGCCACTA TGCCCAGAGA
    AACGACAGAT CCTACTGGCT GGCCAGCGCT GCGCCCCTCC CCATGATGCC ACTCTCTGAA
    GAGGCGATCC GCCCCTATGT CAGCCGCTGT GCGGTATGCG AGGCCCCGGC CCAGGCGGTG
    GCGGTGCACA GCCAGGACCA GTCCATCCCC CCATGTCCGC AGACCTGGAG GAGCCTCTGG
    ATCGGGTATT CATTCCTGAT GCACACAGGA GCTGGGGACC AAGGAGGAGG GCAGGCCCTT
    ATGTCACCTG GCAGCTGCCT GGAAGATTTC AGAGCAGCAC CATTCCTTGA ATGCCAGGGC
    CGGCAGGGAA CTTGCCACTT TTTCGCAAAT AAGTATAGCT TCTGGCTCAC AACGGTGAAA
    GCAGACTTGC AGTTTTCCTC TGCTCCAGCA CCAGACACCT TAAAAGAAAG CCAGGCCCAA
    CGCCAGAAAA TCAGCCGGTG CCAGGTCTGC GTGAAGTATA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508860.1
    CDS327..5291
    Translation

    Target ORF information:

    RefSeq Version XM_011510558.2
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510558.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTTT TCGTGGTGAC ATGGGAGATC CGGGTTTTGG AGGTGAAAAG
    GGGTCCTCCC CTGTTGGGCC CCCAGGCCCT CCCGGCTCAC CAGGAGTGAA TGGTCAGAAA
    GGAATCCCGG GAGACCCTGC ATTTGGTCAC CTGGGACCCC CGGGAAAGAG GGGTCTTTCA
    GGAGTGCCAG GGATAAAAGG ACCCAGAGGT GATCCGGGAT GTCCAGGGGC TGAAGGGCCA
    GCTGGCATTC CTGGATTCCT AGGTCTCAAA GGTCCCAAAG GCAGAGAGGG ACATGCTGGG
    TTTCCAGGTG TCCCAGGTCC ACCTGGCCAT TCCTGTGAAA GAGGTGCTCC AGGGATACCA
    GGGCAACCGG GACTCCCTGG GTATCCAGGT AGCCCAGGTG CTCCAGGTGG GAAAGGACAG
    CCGGGAGATG TGGGGCCTCC CGGGCCAGCT GGAATGAAAG GCCTCCCCGG ACTCCCAGGA
    CGGCCTGGGG CACATGGTCC CCCAGGCCTC CCAGGAATCC CAGGTCCCTT TGGAGATGAT
    GGGCTACCTG GTCCTCCAGG TCCAAAGGGA CCCCGGGGGC TGCCTGGTTT CCCAGGTTTT
    CCCGGAGAAA GAGGAAAGCC TGGTGCAGAG GGATGTCCTG GCGCAAAGGG AGAACCTGGA
    GAGAAGGGCA TGTCTGGCCT TCCTGGAGAC CGGGGACTGA GAGGGGCCAA AGGAGCCATA
    GGACCTCCCG GAGATGAAGG AGAAATGGCT ATCATTTCAC AAAAGGGAAC ACCTGGGGAA
    CCTGGACCTC CTGGAGATGA TGGATTCCCA GGAGAAAGAG GTGATAAAGG AACTCCCGGG
    ATGCAAGGGA GAAGAGGAGA GCCGGGAAGA TACGGACCAC CTGGATTTCA CAGAGGGGAA
    CCTGGTGAGA AAGGTCAGCC AGGGCCTCCT GGACCCCCAG GCCCTCCAGG CTCAACTGGT
    CTAAGAGGGT TCATTGGTTT TCCAGGACTT CCAGGTGACC AGGGTGAGCC AGGTTCTCCA
    GGTCCCCCTG GATTTTCAGG AATTGATGGA GCAAGAGGAC CTAAAGGAAA CAAAGGTGAC
    CCTGCCAGTC ACTTTGGTCC ACCTGGTCCA AAGGGTGAGC CAGGTAGCCC TGGATGTCCA
    GGGCATTTTG GAGCATCCGG AGAGCAGGGC TTGCCTGGTA TTCAAGGGCC CAGAGGATCA
    CCTGGAAGGC CAGGGCCACC TGGCTCCTCT GGACCACCAG GGTGCCCAGG TGATCACGGG
    ATGCCTGGGC TGAGGGGACA GCCAGGAGAA ATGGGAGACC CTGGGCCAAG AGGCCTCCAG
    GGGGATCCAG GGATACCAGG TCCTCCGGGA ATAAAAGGTC CCTCCGGATC ACCTGGCCTG
    AACGGCTTGC ATGGATTGAA AGGTCAGAAA GGAACTAAAG GTGCTTCAGG TTTGCATGAT
    GTGGGGCCAC CTGGTCCAGT GGGAATACCT GGGCTAAAAG GGGAGAGAGG AGACCCTGGG
    AGCCCAGGAA TCTCTCCTCC AGGTCCTCGT GGAAAGAAAG GTCCCCCAGG ACCCCCAGGG
    AGTTCAGGAC CACCTGGTCC TGCAGGTGCC ACAGGAAGAG CTCCTAAGGA CATTCCTGAC
    CCGGGTCCAC CTGGAGATCA GGGACCTCCT GGTCCTGATG GCCCAAGAGG AGCACCTGGG
    CCTCCAGGCC TCCCTGGGAG TGTTGACCTT CTGAGAGGGG AGCCAGGTGA CTGTGGTCTA
    CCAGGGCCAC CAGGTCCCCC TGGCCCACCA GGCCCTCCAG GATACAAAGG CTTTCCAGGA
    TGTGATGGAA AAGATGGCCA GAAAGGACCA GTGGGATTCC CGGGACCGCA GGGACCACAT
    GGATTTCCTG GGCCACCTGG AGAGAAGGGT TTACCTGGAC CTCCAGGGAG AAAAGGGCCC
    ACTGGTCTTC CGGGTCCCAG AGGTGAACCG GGGCCACCTG CAGATGTGGA TGACTGTCCC
    CGAATCCCAG GCCTTCCTGG GGCGCCAGGC ATGAGAGGAC CAGAAGGAGC CATGGGGCTC
    CCTGGAATGA GAGGCCCCTC AGGACCAGGG TGCAAAGGAG AGCCTGGGCT GGATGGCAGG
    AGGGGTGTGG ATGGCGTCCC TGGGTCTCCT GGGCCTCCCG GACGTAAAGG TGACACAGGA
    GAAGACGGCT ACCCTGGAGG ACCAGGGCCT CCTGGTCCCA TTGGGGATCC TGGGCCCAAA
    GGGTTTGGCC CTGGATACCT CGGTGGCTTC CTCCTGGTTC TCCACAGTCA GACGGACCAG
    GAGCCCACCT GCCCCCTGGG CATGCCCAGG CTCTGGACTG GGTATAGTCT GTTATACCTG
    GAAGGGCAAG AGAAAGCTCA CAATCAAGAC CTTGGTCTGG CAGGGTCTTG CCTTCCCGTA
    TTTAGCACGC TGCCCTTTGC CTACTGCAAC ATCCACCAGG TGTGCCACTA TGCCCAGAGA
    AACGACAGAT CCTACTGGCT GGCCAGCGCT GCGCCCCTCC CCATGATGCC ACTCTCTGAA
    GAGGCGATCC GCCCCTATGT CAGCCGCTGT GCGGTATGCG AGGCCCCGGC CCAGGCGGTG
    GCGGTGCACA GCCAGGACCA GTCCATCCCC CCATGTCCGC AGACCTGGAG GAGCCTCTGG
    ATCGGGTATT CATTCCTGAT GCACACAGGA GCTGGGGACC AAGGAGGAGG GCAGGCCCTT
    ATGTCACCTG GCAGCTGCCT GGAAGATTTC AGAGCAGCAC CATTCCTTGA ATGCCAGGGC
    CGGCAGGGAA CTTGCCACTT TTTCGCAAAT AAGTATAGCT TCTGGCTCAC AACGGTGAAA
    GCAGACTTGC AGTTTTCCTC TGCTCCAGCA CCAGACACCT TAAAAGAAAG CCAGGCCCAA
    CGCCAGAAAA TCAGCCGGTG CCAGGTCTGC GTGAAGTATA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56975
    Clone ID Related Accession (Same CDS sequence) XM_011510565.2
    Accession Version XM_011510565.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-4(IV) chain isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510565.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGTGG TGAGTGCCTC TCGCTATGTT TGATGCTACA CACCTGCTGC
    CTGAGCAGCC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508867.1
    CDS327..4601
    Translation

    Target ORF information:

    RefSeq Version XM_011510565.2
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510565.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGTGG TGAGTGCCTC TCGCTATGTT TGATGCTACA CACCTGCTGC
    CTGAGCAGCC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56976
    Clone ID Related Accession (Same CDS sequence) XM_011510568.2
    Accession Version XM_011510568.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-4(IV) chain isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510568.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGTGC TGCCGCTGCT GCTGCTGGTC CAGTAACTAC TCTGATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508870.1
    CDS327..4586
    Translation

    Target ORF information:

    RefSeq Version XM_011510568.2
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510568.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGTGC TGCCGCTGCT GCTGCTGGTC CAGTAACTAC TCTGATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56977
    Clone ID Related Accession (Same CDS sequence) XM_011510570.2 , XM_011510569.2 , XM_017003300.1
    Accession Version XM_011510570.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4245bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-4(IV) chain isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510570.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508872.1
    CDS327..4571
    Translation

    Target ORF information:

    RefSeq Version XM_011510570.2
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510570.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56977
    Clone ID Related Accession (Same CDS sequence) XM_011510570.2 , XM_011510569.2 , XM_017003300.1
    Accession Version XM_011510569.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4245bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-4(IV) chain isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510569.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508871.1
    CDS327..4571
    Translation

    Target ORF information:

    RefSeq Version XM_011510569.2
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510569.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56977
    Clone ID Related Accession (Same CDS sequence) XM_011510570.2 , XM_011510569.2 , XM_017003300.1
    Accession Version XM_017003300.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4245bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-4(IV) chain isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016858789.1
    CDS327..4571
    Translation

    Target ORF information:

    RefSeq Version XM_017003300.1
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017003300.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56978
    Clone ID Related Accession (Same CDS sequence) XM_011510572.3 , XM_011510572.2
    Accession Version XM_011510572.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3399bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-4(IV) chain isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011510572.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTGTAT CAAGAGTTAA AGGGCACAAA GGAGAAAGAG GTCCTGATGG GCCCCCAGGA 
    TTTCCAGGGC AGCCAGGATC ACATGGTCGG GATGGACATG CTGGAGAAAA AGGGGATCCA
    GGACCTCCAG GGGATCATGA AGATGCGACC CCAGGTGGTA AAGGATTTCC TGGACCTCTG
    GGCCCCCCAG GCAAAGCAGG ACCTGTGGGG CCCCCAGGAC TGGGATTTCC TGGTCCACCA
    GGAGAGCGAG GCCACCCAGG AGTTCCAGGC CACCCAGGTG TGAGGGGCCC TGATGGCTTG
    AAGGGTCAGA AAGGTGACAC AATTTCTTGC AACGTAACCT ACCCTGGGAG GCATGGCCCT
    CCAGGTTTTG ATGGACCTCC AGGTCCGAAG GGATTTCCAG GTCCCCAAGG TGCCCCTGGG
    CTGAGTGGTT CAGATGGGCA TAAAGGCAGA CCTGGCACAC CAGGAACAGC GGAAATACCA
    GGTCCACCTG GTTTTCGTGG TGACATGGGA GATCCGGGTT TTGGAGGTGA AAAGGGGTCC
    TCCCCTGTTG GGCCCCCAGG CCCTCCCGGC TCACCAGGAG TGAATGGTCA GAAAGGAATC
    CCGGGAGACC CTGCATTTGG TCACCTGGGA CCCCCGGGAA AGAGGGGTCT TTCAGGAGTG
    CCAGGGATAA AAGGACCCAG AGGTGATCCG GGATGTCCAG GGGCTGAAGG GCCAGCTGGC
    ATTCCTGGAT TCCTAGGTCT CAAAGGTCCC AAAGGCAGAG AGGGACATGC TGGGTTTCCA
    GGTGTCCCAG GTCCACCTGG CCATTCCTGT GAAAGAGGTG CTCCAGGGAT ACCAGGGCAA
    CCGGGACTCC CTGGGTATCC AGGTAGCCCA GGTGCTCCAG GTGGGAAAGG ACAGCCGGGA
    GATGTGGGGC CTCCCGGGCC AGCTGGAATG AAAGGCCTCC CCGGACTCCC AGGACGGCCT
    GGGGCACATG GTCCCCCAGG CCTCCCAGGA ATCCCAGGTC CCTTTGGAGA TGATGGGCTA
    CCTGGTCCTC CAGGTCCAAA GGGACCCCGG GGGCTGCCTG GTTTCCCAGG TTTTCCCGGA
    GAAAGAGGAA AGCCTGGTGC AGAGGGATGT CCTGGCGCAA AGGGAGAACC TGGAGAGAAG
    GGCATGTCTG GCCTTCCTGG AGACCGGGGA CTGAGAGGGG CCAAAGGAGC CATAGGACCT
    CCCGGAGATG AAGGAGAAAT GGCTATCATT TCACAAAAGG GAACACCTGG GGAACCTGGA
    CCTCCTGGAG ATGATGGATT CCCAGGAGAA AGAGGTGATA AAGGAACTCC CGGGATGCAA
    GGGAGAAGAG GAGAGCCGGG AAGATACGGA CCACCTGGAT TTCACAGAGG GGAACCTGGT
    GAGAAAGGTC AGCCAGGGCC TCCTGGACCC CCAGGCCCTC CAGGCTCAAC TGGTCTAAGA
    GGGTTCATTG GTTTTCCAGG ACTTCCAGGT GACCAGGGTG AGCCAGGTTC TCCAGGTCCC
    CCTGGATTTT CAGGAATTGA TGGAGCAAGA GGACCTAAAG GAAACAAAGG TGACCCTGCC
    AGTCACTTTG GTCCACCTGG TCCAAAGGGT GAGCCAGGTA GCCCTGGATG TCCAGGGCAT
    TTTGGAGCAT CCGGAGAGCA GGGCTTGCCT GGTATTCAAG GGCCCAGAGG ATCACCTGGA
    AGGCCAGGGC CACCTGGCTC CTCTGGACCA CCAGGGTGCC CAGGTGATCA CGGGATGCCT
    GGGCTGAGGG GACAGCCAGG AGAAATGGGA GACCCTGGGC CAAGAGGCCT CCAGGGGGAT
    CCAGGGATAC CAGGTCCTCC GGGAATAAAA GGTCCCTCCG GATCACCTGG CCTGAACGGC
    TTGCATGGAT TGAAAGGTCA GAAAGGAACT AAAGGTGCTT CAGGTTTGCA TGATGTGGGG
    CCACCTGGTC CAGTGGGAAT ACCTGGGCTA AAAGGGGAGA GAGGAGACCC TGGGAGCCCA
    GGAATCTCTC CTCCAGGTCC TCGTGGAAAG AAAGGTCCCC CAGGACCCCC AGGGAGTTCA
    GGACCACCTG GTCCTGCAGG TGCCACAGGA AGAGCTCCTA AGGACATTCC TGACCCGGGT
    CCACCTGGAG ATCAGGGACC TCCTGGTCCT GATGGCCCAA GAGGAGCACC TGGGCCTCCA
    GGCCTCCCTG GGAGTGTTGA CCTTCTGAGA GGGGAGCCAG GTGACTGTGG TCTACCAGGG
    CCACCAGGTC CCCCTGGCCC ACCAGGCCCT CCAGGATACA AAGGCTTTCC AGGATGTGAT
    GGAAAAGATG GCCAGAAAGG ACCAGTGGGA TTCCCGGGAC CGCAGGGACC ACATGGATTT
    CCTGGGCCAC CTGGAGAGAA GGGTTTACCT GGACCTCCAG GGAGAAAAGG GCCCACTGGT
    CTTCCGGGTC CCAGAGGTGA ACCGGGGCCA CCTGCAGATG TGGATGACTG TCCCCGAATC
    CCAGGCCTTC CTGGGGCGCC AGGCATGAGA GGACCAGAAG GAGCCATGGG GCTCCCTGGA
    ATGAGAGGCC CCTCAGGACC AGGGTGCAAA GGAGAGCCTG GGCTGGATGG CAGGAGGGGT
    GTGGATGGCG TCCCTGGGTC TCCTGGGCCT CCCGGACGTA AAGGTGACAC AGGAGAAGAC
    GGCTACCCTG GAGGACCAGG GCCTCCTGGT CCCATTGGGG ATCCTGGGCC CAAAGGGTTT
    GGCCCTGGAT ACCTCGGTGG CTTCCTCCTG GTTCTCCACA GTCAGACGGA CCAGGAGCCC
    ACCTGCCCCC TGGGCATGCC CAGGCTCTGG ACTGGGTATA GTCTGTTATA CCTGGAAGGG
    CAAGAGAAAG CTCACAATCA AGACCTTGGT CTGGCAGGGT CTTGCCTTCC CGTATTTAGC
    ACGCTGCCCT TTGCCTACTG CAACATCCAC CAGGTGTGCC ACTATGCCCA GAGAAACGAC
    AGATCCTACT GGCTGGCCAG CGCTGCGCCC CTCCCCATGA TGCCACTCTC TGAAGAGGCG
    ATCCGCCCCT ATGTCAGCCG CTGTGCGGTA TGCGAGGCCC CGGCCCAGGC GGTGGCGGTG
    CACAGCCAGG ACCAGTCCAT CCCCCCATGT CCGCAGACCT GGAGGAGCCT CTGGATCGGG
    TATTCATTCC TGATGCACAC AGGAGCTGGG GACCAAGGAG GAGGGCAGGC CCTTATGTCA
    CCTGGCAGCT GCCTGGAAGA TTTCAGAGCA GCACCATTCC TTGAATGCCA GGGCCGGCAG
    GGAACTTGCC ACTTTTTCGC AAATAAGTAT AGCTTCTGGC TCACAACGGT GAAAGCAGAC
    TTGCAGTTTT CCTCTGCTCC AGCACCAGAC ACCTTAAAAG AAAGCCAGGC CCAACGCCAG
    AAAATCAGCC GGTGCCAGGT CTGCGTGAAG TATAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508874.1
    CDS229..3627
    Translation

    Target ORF information:

    RefSeq Version XM_011510572.3
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510572.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTGTAT CAAGAGTTAA AGGGCACAAA GGAGAAAGAG GTCCTGATGG GCCCCCAGGA 
    TTTCCAGGGC AGCCAGGATC ACATGGTCGG GATGGACATG CTGGAGAAAA AGGGGATCCA
    GGACCTCCAG GGGATCATGA AGATGCGACC CCAGGTGGTA AAGGATTTCC TGGACCTCTG
    GGCCCCCCAG GCAAAGCAGG ACCTGTGGGG CCCCCAGGAC TGGGATTTCC TGGTCCACCA
    GGAGAGCGAG GCCACCCAGG AGTTCCAGGC CACCCAGGTG TGAGGGGCCC TGATGGCTTG
    AAGGGTCAGA AAGGTGACAC AATTTCTTGC AACGTAACCT ACCCTGGGAG GCATGGCCCT
    CCAGGTTTTG ATGGACCTCC AGGTCCGAAG GGATTTCCAG GTCCCCAAGG TGCCCCTGGG
    CTGAGTGGTT CAGATGGGCA TAAAGGCAGA CCTGGCACAC CAGGAACAGC GGAAATACCA
    GGTCCACCTG GTTTTCGTGG TGACATGGGA GATCCGGGTT TTGGAGGTGA AAAGGGGTCC
    TCCCCTGTTG GGCCCCCAGG CCCTCCCGGC TCACCAGGAG TGAATGGTCA GAAAGGAATC
    CCGGGAGACC CTGCATTTGG TCACCTGGGA CCCCCGGGAA AGAGGGGTCT TTCAGGAGTG
    CCAGGGATAA AAGGACCCAG AGGTGATCCG GGATGTCCAG GGGCTGAAGG GCCAGCTGGC
    ATTCCTGGAT TCCTAGGTCT CAAAGGTCCC AAAGGCAGAG AGGGACATGC TGGGTTTCCA
    GGTGTCCCAG GTCCACCTGG CCATTCCTGT GAAAGAGGTG CTCCAGGGAT ACCAGGGCAA
    CCGGGACTCC CTGGGTATCC AGGTAGCCCA GGTGCTCCAG GTGGGAAAGG ACAGCCGGGA
    GATGTGGGGC CTCCCGGGCC AGCTGGAATG AAAGGCCTCC CCGGACTCCC AGGACGGCCT
    GGGGCACATG GTCCCCCAGG CCTCCCAGGA ATCCCAGGTC CCTTTGGAGA TGATGGGCTA
    CCTGGTCCTC CAGGTCCAAA GGGACCCCGG GGGCTGCCTG GTTTCCCAGG TTTTCCCGGA
    GAAAGAGGAA AGCCTGGTGC AGAGGGATGT CCTGGCGCAA AGGGAGAACC TGGAGAGAAG
    GGCATGTCTG GCCTTCCTGG AGACCGGGGA CTGAGAGGGG CCAAAGGAGC CATAGGACCT
    CCCGGAGATG AAGGAGAAAT GGCTATCATT TCACAAAAGG GAACACCTGG GGAACCTGGA
    CCTCCTGGAG ATGATGGATT CCCAGGAGAA AGAGGTGATA AAGGAACTCC CGGGATGCAA
    GGGAGAAGAG GAGAGCCGGG AAGATACGGA CCACCTGGAT TTCACAGAGG GGAACCTGGT
    GAGAAAGGTC AGCCAGGGCC TCCTGGACCC CCAGGCCCTC CAGGCTCAAC TGGTCTAAGA
    GGGTTCATTG GTTTTCCAGG ACTTCCAGGT GACCAGGGTG AGCCAGGTTC TCCAGGTCCC
    CCTGGATTTT CAGGAATTGA TGGAGCAAGA GGACCTAAAG GAAACAAAGG TGACCCTGCC
    AGTCACTTTG GTCCACCTGG TCCAAAGGGT GAGCCAGGTA GCCCTGGATG TCCAGGGCAT
    TTTGGAGCAT CCGGAGAGCA GGGCTTGCCT GGTATTCAAG GGCCCAGAGG ATCACCTGGA
    AGGCCAGGGC CACCTGGCTC CTCTGGACCA CCAGGGTGCC CAGGTGATCA CGGGATGCCT
    GGGCTGAGGG GACAGCCAGG AGAAATGGGA GACCCTGGGC CAAGAGGCCT CCAGGGGGAT
    CCAGGGATAC CAGGTCCTCC GGGAATAAAA GGTCCCTCCG GATCACCTGG CCTGAACGGC
    TTGCATGGAT TGAAAGGTCA GAAAGGAACT AAAGGTGCTT CAGGTTTGCA TGATGTGGGG
    CCACCTGGTC CAGTGGGAAT ACCTGGGCTA AAAGGGGAGA GAGGAGACCC TGGGAGCCCA
    GGAATCTCTC CTCCAGGTCC TCGTGGAAAG AAAGGTCCCC CAGGACCCCC AGGGAGTTCA
    GGACCACCTG GTCCTGCAGG TGCCACAGGA AGAGCTCCTA AGGACATTCC TGACCCGGGT
    CCACCTGGAG ATCAGGGACC TCCTGGTCCT GATGGCCCAA GAGGAGCACC TGGGCCTCCA
    GGCCTCCCTG GGAGTGTTGA CCTTCTGAGA GGGGAGCCAG GTGACTGTGG TCTACCAGGG
    CCACCAGGTC CCCCTGGCCC ACCAGGCCCT CCAGGATACA AAGGCTTTCC AGGATGTGAT
    GGAAAAGATG GCCAGAAAGG ACCAGTGGGA TTCCCGGGAC CGCAGGGACC ACATGGATTT
    CCTGGGCCAC CTGGAGAGAA GGGTTTACCT GGACCTCCAG GGAGAAAAGG GCCCACTGGT
    CTTCCGGGTC CCAGAGGTGA ACCGGGGCCA CCTGCAGATG TGGATGACTG TCCCCGAATC
    CCAGGCCTTC CTGGGGCGCC AGGCATGAGA GGACCAGAAG GAGCCATGGG GCTCCCTGGA
    ATGAGAGGCC CCTCAGGACC AGGGTGCAAA GGAGAGCCTG GGCTGGATGG CAGGAGGGGT
    GTGGATGGCG TCCCTGGGTC TCCTGGGCCT CCCGGACGTA AAGGTGACAC AGGAGAAGAC
    GGCTACCCTG GAGGACCAGG GCCTCCTGGT CCCATTGGGG ATCCTGGGCC CAAAGGGTTT
    GGCCCTGGAT ACCTCGGTGG CTTCCTCCTG GTTCTCCACA GTCAGACGGA CCAGGAGCCC
    ACCTGCCCCC TGGGCATGCC CAGGCTCTGG ACTGGGTATA GTCTGTTATA CCTGGAAGGG
    CAAGAGAAAG CTCACAATCA AGACCTTGGT CTGGCAGGGT CTTGCCTTCC CGTATTTAGC
    ACGCTGCCCT TTGCCTACTG CAACATCCAC CAGGTGTGCC ACTATGCCCA GAGAAACGAC
    AGATCCTACT GGCTGGCCAG CGCTGCGCCC CTCCCCATGA TGCCACTCTC TGAAGAGGCG
    ATCCGCCCCT ATGTCAGCCG CTGTGCGGTA TGCGAGGCCC CGGCCCAGGC GGTGGCGGTG
    CACAGCCAGG ACCAGTCCAT CCCCCCATGT CCGCAGACCT GGAGGAGCCT CTGGATCGGG
    TATTCATTCC TGATGCACAC AGGAGCTGGG GACCAAGGAG GAGGGCAGGC CCTTATGTCA
    CCTGGCAGCT GCCTGGAAGA TTTCAGAGCA GCACCATTCC TTGAATGCCA GGGCCGGCAG
    GGAACTTGCC ACTTTTTCGC AAATAAGTAT AGCTTCTGGC TCACAACGGT GAAAGCAGAC
    TTGCAGTTTT CCTCTGCTCC AGCACCAGAC ACCTTAAAAG AAAGCCAGGC CCAACGCCAG
    AAAATCAGCC GGTGCCAGGT CTGCGTGAAG TATAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56978
    Clone ID Related Accession (Same CDS sequence) XM_011510572.3 , XM_011510572.2
    Accession Version XM_011510572.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3399bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product collagen alpha-4(IV) chain isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510572.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTGTAT CAAGAGTTAA AGGGCACAAA GGAGAAAGAG GTCCTGATGG GCCCCCAGGA 
    TTTCCAGGGC AGCCAGGATC ACATGGTCGG GATGGACATG CTGGAGAAAA AGGGGATCCA
    GGACCTCCAG GGGATCATGA AGATGCGACC CCAGGTGGTA AAGGATTTCC TGGACCTCTG
    GGCCCCCCAG GCAAAGCAGG ACCTGTGGGG CCCCCAGGAC TGGGATTTCC TGGTCCACCA
    GGAGAGCGAG GCCACCCAGG AGTTCCAGGC CACCCAGGTG TGAGGGGCCC TGATGGCTTG
    AAGGGTCAGA AAGGTGACAC AATTTCTTGC AACGTAACCT ACCCTGGGAG GCATGGCCCT
    CCAGGTTTTG ATGGACCTCC AGGTCCGAAG GGATTTCCAG GTCCCCAAGG TGCCCCTGGG
    CTGAGTGGTT CAGATGGGCA TAAAGGCAGA CCTGGCACAC CAGGAACAGC GGAAATACCA
    GGTCCACCTG GTTTTCGTGG TGACATGGGA GATCCGGGTT TTGGAGGTGA AAAGGGGTCC
    TCCCCTGTTG GGCCCCCAGG CCCTCCCGGC TCACCAGGAG TGAATGGTCA GAAAGGAATC
    CCGGGAGACC CTGCATTTGG TCACCTGGGA CCCCCGGGAA AGAGGGGTCT TTCAGGAGTG
    CCAGGGATAA AAGGACCCAG AGGTGATCCG GGATGTCCAG GGGCTGAAGG GCCAGCTGGC
    ATTCCTGGAT TCCTAGGTCT CAAAGGTCCC AAAGGCAGAG AGGGACATGC TGGGTTTCCA
    GGTGTCCCAG GTCCACCTGG CCATTCCTGT GAAAGAGGTG CTCCAGGGAT ACCAGGGCAA
    CCGGGACTCC CTGGGTATCC AGGTAGCCCA GGTGCTCCAG GTGGGAAAGG ACAGCCGGGA
    GATGTGGGGC CTCCCGGGCC AGCTGGAATG AAAGGCCTCC CCGGACTCCC AGGACGGCCT
    GGGGCACATG GTCCCCCAGG CCTCCCAGGA ATCCCAGGTC CCTTTGGAGA TGATGGGCTA
    CCTGGTCCTC CAGGTCCAAA GGGACCCCGG GGGCTGCCTG GTTTCCCAGG TTTTCCCGGA
    GAAAGAGGAA AGCCTGGTGC AGAGGGATGT CCTGGCGCAA AGGGAGAACC TGGAGAGAAG
    GGCATGTCTG GCCTTCCTGG AGACCGGGGA CTGAGAGGGG CCAAAGGAGC CATAGGACCT
    CCCGGAGATG AAGGAGAAAT GGCTATCATT TCACAAAAGG GAACACCTGG GGAACCTGGA
    CCTCCTGGAG ATGATGGATT CCCAGGAGAA AGAGGTGATA AAGGAACTCC CGGGATGCAA
    GGGAGAAGAG GAGAGCCGGG AAGATACGGA CCACCTGGAT TTCACAGAGG GGAACCTGGT
    GAGAAAGGTC AGCCAGGGCC TCCTGGACCC CCAGGCCCTC CAGGCTCAAC TGGTCTAAGA
    GGGTTCATTG GTTTTCCAGG ACTTCCAGGT GACCAGGGTG AGCCAGGTTC TCCAGGTCCC
    CCTGGATTTT CAGGAATTGA TGGAGCAAGA GGACCTAAAG GAAACAAAGG TGACCCTGCC
    AGTCACTTTG GTCCACCTGG TCCAAAGGGT GAGCCAGGTA GCCCTGGATG TCCAGGGCAT
    TTTGGAGCAT CCGGAGAGCA GGGCTTGCCT GGTATTCAAG GGCCCAGAGG ATCACCTGGA
    AGGCCAGGGC CACCTGGCTC CTCTGGACCA CCAGGGTGCC CAGGTGATCA CGGGATGCCT
    GGGCTGAGGG GACAGCCAGG AGAAATGGGA GACCCTGGGC CAAGAGGCCT CCAGGGGGAT
    CCAGGGATAC CAGGTCCTCC GGGAATAAAA GGTCCCTCCG GATCACCTGG CCTGAACGGC
    TTGCATGGAT TGAAAGGTCA GAAAGGAACT AAAGGTGCTT CAGGTTTGCA TGATGTGGGG
    CCACCTGGTC CAGTGGGAAT ACCTGGGCTA AAAGGGGAGA GAGGAGACCC TGGGAGCCCA
    GGAATCTCTC CTCCAGGTCC TCGTGGAAAG AAAGGTCCCC CAGGACCCCC AGGGAGTTCA
    GGACCACCTG GTCCTGCAGG TGCCACAGGA AGAGCTCCTA AGGACATTCC TGACCCGGGT
    CCACCTGGAG ATCAGGGACC TCCTGGTCCT GATGGCCCAA GAGGAGCACC TGGGCCTCCA
    GGCCTCCCTG GGAGTGTTGA CCTTCTGAGA GGGGAGCCAG GTGACTGTGG TCTACCAGGG
    CCACCAGGTC CCCCTGGCCC ACCAGGCCCT CCAGGATACA AAGGCTTTCC AGGATGTGAT
    GGAAAAGATG GCCAGAAAGG ACCAGTGGGA TTCCCGGGAC CGCAGGGACC ACATGGATTT
    CCTGGGCCAC CTGGAGAGAA GGGTTTACCT GGACCTCCAG GGAGAAAAGG GCCCACTGGT
    CTTCCGGGTC CCAGAGGTGA ACCGGGGCCA CCTGCAGATG TGGATGACTG TCCCCGAATC
    CCAGGCCTTC CTGGGGCGCC AGGCATGAGA GGACCAGAAG GAGCCATGGG GCTCCCTGGA
    ATGAGAGGCC CCTCAGGACC AGGGTGCAAA GGAGAGCCTG GGCTGGATGG CAGGAGGGGT
    GTGGATGGCG TCCCTGGGTC TCCTGGGCCT CCCGGACGTA AAGGTGACAC AGGAGAAGAC
    GGCTACCCTG GAGGACCAGG GCCTCCTGGT CCCATTGGGG ATCCTGGGCC CAAAGGGTTT
    GGCCCTGGAT ACCTCGGTGG CTTCCTCCTG GTTCTCCACA GTCAGACGGA CCAGGAGCCC
    ACCTGCCCCC TGGGCATGCC CAGGCTCTGG ACTGGGTATA GTCTGTTATA CCTGGAAGGG
    CAAGAGAAAG CTCACAATCA AGACCTTGGT CTGGCAGGGT CTTGCCTTCC CGTATTTAGC
    ACGCTGCCCT TTGCCTACTG CAACATCCAC CAGGTGTGCC ACTATGCCCA GAGAAACGAC
    AGATCCTACT GGCTGGCCAG CGCTGCGCCC CTCCCCATGA TGCCACTCTC TGAAGAGGCG
    ATCCGCCCCT ATGTCAGCCG CTGTGCGGTA TGCGAGGCCC CGGCCCAGGC GGTGGCGGTG
    CACAGCCAGG ACCAGTCCAT CCCCCCATGT CCGCAGACCT GGAGGAGCCT CTGGATCGGG
    TATTCATTCC TGATGCACAC AGGAGCTGGG GACCAAGGAG GAGGGCAGGC CCTTATGTCA
    CCTGGCAGCT GCCTGGAAGA TTTCAGAGCA GCACCATTCC TTGAATGCCA GGGCCGGCAG
    GGAACTTGCC ACTTTTTCGC AAATAAGTAT AGCTTCTGGC TCACAACGGT GAAAGCAGAC
    TTGCAGTTTT CCTCTGCTCC AGCACCAGAC ACCTTAAAAG AAAGCCAGGC CCAACGCCAG
    AAAATCAGCC GGTGCCAGGT CTGCGTGAAG TATAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508874.1
    CDS226..3624
    Translation

    Target ORF information:

    RefSeq Version XM_011510572.2
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 4 (COL4A4), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510572.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTGTAT CAAGAGTTAA AGGGCACAAA GGAGAAAGAG GTCCTGATGG GCCCCCAGGA 
    TTTCCAGGGC AGCCAGGATC ACATGGTCGG GATGGACATG CTGGAGAAAA AGGGGATCCA
    GGACCTCCAG GGGATCATGA AGATGCGACC CCAGGTGGTA AAGGATTTCC TGGACCTCTG
    GGCCCCCCAG GCAAAGCAGG ACCTGTGGGG CCCCCAGGAC TGGGATTTCC TGGTCCACCA
    GGAGAGCGAG GCCACCCAGG AGTTCCAGGC CACCCAGGTG TGAGGGGCCC TGATGGCTTG
    AAGGGTCAGA AAGGTGACAC AATTTCTTGC AACGTAACCT ACCCTGGGAG GCATGGCCCT
    CCAGGTTTTG ATGGACCTCC AGGTCCGAAG GGATTTCCAG GTCCCCAAGG TGCCCCTGGG
    CTGAGTGGTT CAGATGGGCA TAAAGGCAGA CCTGGCACAC CAGGAACAGC GGAAATACCA
    GGTCCACCTG GTTTTCGTGG TGACATGGGA GATCCGGGTT TTGGAGGTGA AAAGGGGTCC
    TCCCCTGTTG GGCCCCCAGG CCCTCCCGGC TCACCAGGAG TGAATGGTCA GAAAGGAATC
    CCGGGAGACC CTGCATTTGG TCACCTGGGA CCCCCGGGAA AGAGGGGTCT TTCAGGAGTG
    CCAGGGATAA AAGGACCCAG AGGTGATCCG GGATGTCCAG GGGCTGAAGG GCCAGCTGGC
    ATTCCTGGAT TCCTAGGTCT CAAAGGTCCC AAAGGCAGAG AGGGACATGC TGGGTTTCCA
    GGTGTCCCAG GTCCACCTGG CCATTCCTGT GAAAGAGGTG CTCCAGGGAT ACCAGGGCAA
    CCGGGACTCC CTGGGTATCC AGGTAGCCCA GGTGCTCCAG GTGGGAAAGG ACAGCCGGGA
    GATGTGGGGC CTCCCGGGCC AGCTGGAATG AAAGGCCTCC CCGGACTCCC AGGACGGCCT
    GGGGCACATG GTCCCCCAGG CCTCCCAGGA ATCCCAGGTC CCTTTGGAGA TGATGGGCTA
    CCTGGTCCTC CAGGTCCAAA GGGACCCCGG GGGCTGCCTG GTTTCCCAGG TTTTCCCGGA
    GAAAGAGGAA AGCCTGGTGC AGAGGGATGT CCTGGCGCAA AGGGAGAACC TGGAGAGAAG
    GGCATGTCTG GCCTTCCTGG AGACCGGGGA CTGAGAGGGG CCAAAGGAGC CATAGGACCT
    CCCGGAGATG AAGGAGAAAT GGCTATCATT TCACAAAAGG GAACACCTGG GGAACCTGGA
    CCTCCTGGAG ATGATGGATT CCCAGGAGAA AGAGGTGATA AAGGAACTCC CGGGATGCAA
    GGGAGAAGAG GAGAGCCGGG AAGATACGGA CCACCTGGAT TTCACAGAGG GGAACCTGGT
    GAGAAAGGTC AGCCAGGGCC TCCTGGACCC CCAGGCCCTC CAGGCTCAAC TGGTCTAAGA
    GGGTTCATTG GTTTTCCAGG ACTTCCAGGT GACCAGGGTG AGCCAGGTTC TCCAGGTCCC
    CCTGGATTTT CAGGAATTGA TGGAGCAAGA GGACCTAAAG GAAACAAAGG TGACCCTGCC
    AGTCACTTTG GTCCACCTGG TCCAAAGGGT GAGCCAGGTA GCCCTGGATG TCCAGGGCAT
    TTTGGAGCAT CCGGAGAGCA GGGCTTGCCT GGTATTCAAG GGCCCAGAGG ATCACCTGGA
    AGGCCAGGGC CACCTGGCTC CTCTGGACCA CCAGGGTGCC CAGGTGATCA CGGGATGCCT
    GGGCTGAGGG GACAGCCAGG AGAAATGGGA GACCCTGGGC CAAGAGGCCT CCAGGGGGAT
    CCAGGGATAC CAGGTCCTCC GGGAATAAAA GGTCCCTCCG GATCACCTGG CCTGAACGGC
    TTGCATGGAT TGAAAGGTCA GAAAGGAACT AAAGGTGCTT CAGGTTTGCA TGATGTGGGG
    CCACCTGGTC CAGTGGGAAT ACCTGGGCTA AAAGGGGAGA GAGGAGACCC TGGGAGCCCA
    GGAATCTCTC CTCCAGGTCC TCGTGGAAAG AAAGGTCCCC CAGGACCCCC AGGGAGTTCA
    GGACCACCTG GTCCTGCAGG TGCCACAGGA AGAGCTCCTA AGGACATTCC TGACCCGGGT
    CCACCTGGAG ATCAGGGACC TCCTGGTCCT GATGGCCCAA GAGGAGCACC TGGGCCTCCA
    GGCCTCCCTG GGAGTGTTGA CCTTCTGAGA GGGGAGCCAG GTGACTGTGG TCTACCAGGG
    CCACCAGGTC CCCCTGGCCC ACCAGGCCCT CCAGGATACA AAGGCTTTCC AGGATGTGAT
    GGAAAAGATG GCCAGAAAGG ACCAGTGGGA TTCCCGGGAC CGCAGGGACC ACATGGATTT
    CCTGGGCCAC CTGGAGAGAA GGGTTTACCT GGACCTCCAG GGAGAAAAGG GCCCACTGGT
    CTTCCGGGTC CCAGAGGTGA ACCGGGGCCA CCTGCAGATG TGGATGACTG TCCCCGAATC
    CCAGGCCTTC CTGGGGCGCC AGGCATGAGA GGACCAGAAG GAGCCATGGG GCTCCCTGGA
    ATGAGAGGCC CCTCAGGACC AGGGTGCAAA GGAGAGCCTG GGCTGGATGG CAGGAGGGGT
    GTGGATGGCG TCCCTGGGTC TCCTGGGCCT CCCGGACGTA AAGGTGACAC AGGAGAAGAC
    GGCTACCCTG GAGGACCAGG GCCTCCTGGT CCCATTGGGG ATCCTGGGCC CAAAGGGTTT
    GGCCCTGGAT ACCTCGGTGG CTTCCTCCTG GTTCTCCACA GTCAGACGGA CCAGGAGCCC
    ACCTGCCCCC TGGGCATGCC CAGGCTCTGG ACTGGGTATA GTCTGTTATA CCTGGAAGGG
    CAAGAGAAAG CTCACAATCA AGACCTTGGT CTGGCAGGGT CTTGCCTTCC CGTATTTAGC
    ACGCTGCCCT TTGCCTACTG CAACATCCAC CAGGTGTGCC ACTATGCCCA GAGAAACGAC
    AGATCCTACT GGCTGGCCAG CGCTGCGCCC CTCCCCATGA TGCCACTCTC TGAAGAGGCG
    ATCCGCCCCT ATGTCAGCCG CTGTGCGGTA TGCGAGGCCC CGGCCCAGGC GGTGGCGGTG
    CACAGCCAGG ACCAGTCCAT CCCCCCATGT CCGCAGACCT GGAGGAGCCT CTGGATCGGG
    TATTCATTCC TGATGCACAC AGGAGCTGGG GACCAAGGAG GAGGGCAGGC CCTTATGTCA
    CCTGGCAGCT GCCTGGAAGA TTTCAGAGCA GCACCATTCC TTGAATGCCA GGGCCGGCAG
    GGAACTTGCC ACTTTTTCGC AAATAAGTAT AGCTTCTGGC TCACAACGGT GAAAGCAGAC
    TTGCAGTTTT CCTCTGCTCC AGCACCAGAC ACCTTAAAAG AAAGCCAGGC CCAACGCCAG
    AAAATCAGCC GGTGCCAGGT CTGCGTGAAG TATAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu88997
    Clone ID Related Accession (Same CDS sequence) XM_017003296.1
    Accession Version XM_017003296.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4980bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-4(IV) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT
    GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC
    CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT
    GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC
    CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG
    AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG
    CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC
    TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC
    CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC
    CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA
    TTCCTGATGG TGAGTCCCGT GCCCTTGAAA ACCCTGGCTC ATTCACCATT TCCTAACACC
    TTCTCCCGCC ATTTCTCTCT GGATTGGACA GTAATTCTGA GCACTCTGCG CTTAGGACAT
    AAGGTTACGG GGACTGTTGC AAACCATAGC CTGTTGGGAT GCAGGGCCAT TATCTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016858785.1
    CDS327..5306
    Translation

    Target ORF information:

    RefSeq Version XM_017003296.1
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017003296.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT
    GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC
    CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT
    GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC
    CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG
    AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG
    CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC
    TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC
    CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC
    CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA
    TTCCTGATGG TGAGTCCCGT GCCCTTGAAA ACCCTGGCTC ATTCACCATT TCCTAACACC
    TTCTCCCGCC ATTTCTCTCT GGATTGGACA GTAATTCTGA GCACTCTGCG CTTAGGACAT
    AAGGTTACGG GGACTGTTGC AAACCATAGC CTGTTGGGAT GCAGGGCCAT TATCTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu88998
    Clone ID Related Accession (Same CDS sequence) XM_017003297.1
    Accession Version XM_017003297.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-4(IV) chain isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGGCCAC CTGGCTCCTC TGGACCACCA GGGTGCCCAG GTGATCACGG GATGCCTGGG
    CTGAGGGGAC AGCCAGGAGA AATGGGAGAC CCTGGGCCAA GAGGCCTCCA GGGGGATCCA
    GGGATACCAG GTCCTCCGGG AATAAAAGGT CCCTCCGGAT CACCTGGCCT GAACGGCTTG
    CATGGATTGA AAGGTCAGAA AGGAACTAAA GGTGCTTCAG GTTTGCATGA TGTGGGGCCA
    CCTGGTCCAG TGGGAATACC TGGGCTAAAA GGGGAGAGAG GAGACCCTGG GAGCCCAGGA
    ATCTCTCCTC CAGGTCCTCG TGGAAAGAAA GGTCCCCCAG GACCCCCAGG GAGTTCAGGA
    CCACCTGGTC CTGCAGGTGC CACAGGAAGA GCTCCTAAGG ACATTCCTGA CCCGGGTCCA
    CCTGGAGATC AGGGACCTCC TGGTCCTGAT GGCCCAAGAG GAGCACCTGG GCCTCCAGGC
    CTCCCTGGGA GTGTTGACCT TCTGAGAGGG GAGCCAGGTG ACTGTGGTCT ACCAGGGCCA
    CCAGGTCCCC CTGGCCCACC AGGCCCTCCA GGATACAAAG GCTTTCCAGG ATGTGATGGA
    AAAGATGGCC AGAAAGGACC AGTGGGATTC CCGGGACCGC AGGGACCACA TGGATTTCCT
    GGGCCACCTG GAGAGAAGGG TTTACCTGGA CCTCCAGGGA GAAAAGGGCC CACTGGTCTT
    CCGGGTCCCA GAGGTGAACC GGGGCCACCT GCAGATGTGG ATGACTGTCC CCGAATCCCA
    GGCCTTCCTG GGGCGCCAGG CATGAGAGGA CCAGAAGGAG CCATGGGGCT CCCTGGAATG
    AGAGGCCCCT CAGGACCAGG GTGCAAAGGA GAGCCTGGGC TGGATGGCAG GAGGGGTGTG
    GATGGCGTCC CTGGGTCTCC TGGGCCTCCC GGACGTAAAG GTGACACAGG AGAAGACGGC
    TACCCTGGAG GACCAGGGCC TCCTGGTCCC ATTGGGGATC CTGGGCCCAA AGGGTTTGGC
    CCTGGATACC TCGGTGGCTT CCTCCTGGTT CTCCACAGTC AGACGGACCA GGAGCCCACC
    TGCCCCCTGG GCATGCCCAG GCTCTGGACT GGGTATAGTC TGTTATACCT GGAAGGGCAA
    GAGAAAGCTC ACAATCAAGA CCTTGGTCTG GCAGGGTCTT GCCTTCCCGT ATTTAGCACG
    CTGCCCTTTG CCTACTGCAA CATCCACCAG GTGTGCCACT ATGCCCAGAG AAACGACAGA
    TCCTACTGGC TGGCCAGCGC TGCGCCCCTC CCCATGATGC CACTCTCTGA AGAGGCGATC
    CGCCCCTATG TCAGCCGCTG TGCGGTATGC GAGGCCCCGG CCCAGGCGGT GGCGGTGCAC
    AGCCAGGACC AGTCCATCCC CCCATGTCCG CAGACCTGGA GGAGCCTCTG GATCGGGTAT
    TCATTCCTGA TGCACACAGG AGCTGGGGAC CAAGGAGGAG GGCAGGCCCT TATGTCACCT
    GGCAGCTGCC TGGAAGATTT CAGAGCAGCA CCATTCCTTG AATGCCAGGG CCGGCAGGGA
    ACTTGCCACT TTTTCGCAAA TAAGTATAGC TTCTGGCTCA CAACGGTGAA AGCAGACTTG
    CAGTTTTCCT CTGCTCCAGC ACCAGACACC TTAAAAGAAA GCCAGGCCCA ACGCCAGAAA
    ATCAGCCGGT GCCAGGTCTG CGTGAAGTAT AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016858786.1
    CDS327..5282
    Translation

    Target ORF information:

    RefSeq Version XM_017003297.1
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017003297.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGGCCAC CTGGCTCCTC TGGACCACCA GGGTGCCCAG GTGATCACGG GATGCCTGGG
    CTGAGGGGAC AGCCAGGAGA AATGGGAGAC CCTGGGCCAA GAGGCCTCCA GGGGGATCCA
    GGGATACCAG GTCCTCCGGG AATAAAAGGT CCCTCCGGAT CACCTGGCCT GAACGGCTTG
    CATGGATTGA AAGGTCAGAA AGGAACTAAA GGTGCTTCAG GTTTGCATGA TGTGGGGCCA
    CCTGGTCCAG TGGGAATACC TGGGCTAAAA GGGGAGAGAG GAGACCCTGG GAGCCCAGGA
    ATCTCTCCTC CAGGTCCTCG TGGAAAGAAA GGTCCCCCAG GACCCCCAGG GAGTTCAGGA
    CCACCTGGTC CTGCAGGTGC CACAGGAAGA GCTCCTAAGG ACATTCCTGA CCCGGGTCCA
    CCTGGAGATC AGGGACCTCC TGGTCCTGAT GGCCCAAGAG GAGCACCTGG GCCTCCAGGC
    CTCCCTGGGA GTGTTGACCT TCTGAGAGGG GAGCCAGGTG ACTGTGGTCT ACCAGGGCCA
    CCAGGTCCCC CTGGCCCACC AGGCCCTCCA GGATACAAAG GCTTTCCAGG ATGTGATGGA
    AAAGATGGCC AGAAAGGACC AGTGGGATTC CCGGGACCGC AGGGACCACA TGGATTTCCT
    GGGCCACCTG GAGAGAAGGG TTTACCTGGA CCTCCAGGGA GAAAAGGGCC CACTGGTCTT
    CCGGGTCCCA GAGGTGAACC GGGGCCACCT GCAGATGTGG ATGACTGTCC CCGAATCCCA
    GGCCTTCCTG GGGCGCCAGG CATGAGAGGA CCAGAAGGAG CCATGGGGCT CCCTGGAATG
    AGAGGCCCCT CAGGACCAGG GTGCAAAGGA GAGCCTGGGC TGGATGGCAG GAGGGGTGTG
    GATGGCGTCC CTGGGTCTCC TGGGCCTCCC GGACGTAAAG GTGACACAGG AGAAGACGGC
    TACCCTGGAG GACCAGGGCC TCCTGGTCCC ATTGGGGATC CTGGGCCCAA AGGGTTTGGC
    CCTGGATACC TCGGTGGCTT CCTCCTGGTT CTCCACAGTC AGACGGACCA GGAGCCCACC
    TGCCCCCTGG GCATGCCCAG GCTCTGGACT GGGTATAGTC TGTTATACCT GGAAGGGCAA
    GAGAAAGCTC ACAATCAAGA CCTTGGTCTG GCAGGGTCTT GCCTTCCCGT ATTTAGCACG
    CTGCCCTTTG CCTACTGCAA CATCCACCAG GTGTGCCACT ATGCCCAGAG AAACGACAGA
    TCCTACTGGC TGGCCAGCGC TGCGCCCCTC CCCATGATGC CACTCTCTGA AGAGGCGATC
    CGCCCCTATG TCAGCCGCTG TGCGGTATGC GAGGCCCCGG CCCAGGCGGT GGCGGTGCAC
    AGCCAGGACC AGTCCATCCC CCCATGTCCG CAGACCTGGA GGAGCCTCTG GATCGGGTAT
    TCATTCCTGA TGCACACAGG AGCTGGGGAC CAAGGAGGAG GGCAGGCCCT TATGTCACCT
    GGCAGCTGCC TGGAAGATTT CAGAGCAGCA CCATTCCTTG AATGCCAGGG CCGGCAGGGA
    ACTTGCCACT TTTTCGCAAA TAAGTATAGC TTCTGGCTCA CAACGGTGAA AGCAGACTTG
    CAGTTTTCCT CTGCTCCAGC ACCAGACACC TTAAAAGAAA GCCAGGCCCA ACGCCAGAAA
    ATCAGCCGGT GCCAGGTCTG CGTGAAGTAT AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu88999
    Clone ID Related Accession (Same CDS sequence) XM_017003299.1
    Accession Version XM_017003299.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4557bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-4(IV) chain isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT
    GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC
    CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT
    GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC
    CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG
    AAAGCTCACA ATCAAGACCT TGGTATGGAT ACAGGATCGC CCATCTCCAT TGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016858788.1
    CDS327..4883
    Translation

    Target ORF information:

    RefSeq Version XM_017003299.1
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017003299.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
    ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA
    AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA
    AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA
    GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT
    GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA
    GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC
    CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA
    GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT
    GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT
    TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA
    CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG
    CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA
    GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG
    GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA
    AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT
    TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT
    GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT
    CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA
    ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT
    GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA
    GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA
    CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT
    AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA
    GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC
    CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT
    GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA
    CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT
    GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA
    GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT
    CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC
    CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG
    CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT
    CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT
    TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT
    GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA
    CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT
    GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA
    GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG
    ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT
    GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC
    CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA
    CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG
    GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA
    CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT
    CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA
    GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG
    TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA
    GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT
    GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA
    AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA
    GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC
    ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA
    TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC
    TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA
    GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA
    GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG
    AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG
    ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT
    GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT
    GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC
    TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA
    CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT
    GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC
    CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA
    GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA
    GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG
    CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG
    GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC
    CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA
    GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT
    GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC
    CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT
    GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC
    CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG
    AAAGCTCACA ATCAAGACCT TGGTATGGAT ACAGGATCGC CCATCTCCAT TGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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