CSF1 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following CSF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CSF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu10466 NM_172210.2
Latest version!
Homo sapiens colony stimulating factor 1 (CSF1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$184.50-$258.30
$369.00
OHu11756 NM_000757.5
Latest version!
Homo sapiens colony stimulating factor 1 (CSF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu11756 NM_172212.2
Latest version!
Homo sapiens colony stimulating factor 1 (CSF1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu12311 NM_172211.3
Latest version!
Homo sapiens colony stimulating factor 1 (CSF1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu108521 NM_172211.4
Latest version!
Homo sapiens colony stimulating factor 1 (CSF1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OHu108673 NM_172210.3
Latest version!
Homo sapiens colony stimulating factor 1 (CSF1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu107858 NM_172212.3
Latest version!
Homo sapiens colony stimulating factor 1 (CSF1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu107858 NM_000757.6
Latest version!
Homo sapiens colony stimulating factor 1 (CSF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu83176 XM_017000369.1
Latest version!
Homo sapiens colony stimulating factor 1 (CSF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu10466
    Clone ID Related Accession (Same CDS sequence) NM_172210.2
    Accession Version NM_172210.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1317bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-20
    Organism Homo sapiens(human)
    Product macrophage colony-stimulating factor 1 isoform b precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA414355.1, BC021117.1, U22386.1 and M27087.1. On Jan 30, 2008 this sequence version replaced NM_172210.1. Transcript Variant: This variant (2) lacks an alternate in-frame segment compared to variant 1, resulting in a shorter isoform (b) compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U22386.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal (PMID:3500041) ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACCGCGC CGGGCGCCGC CGGGCGCTGC CCTCCCACGA CATGGCTGGG CTCCCTGCTG 
    TTGTTGGTCT GTCTCCTGGC GAGCAGGAGT ATCACCGAGG AGGTGTCGGA GTACTGTAGC
    CACATGATTG GGAGTGGACA CCTGCAGTCT CTGCAGCGGC TGATTGACAG TCAGATGGAG
    ACCTCGTGCC AAATTACATT TGAGTTTGTA GACCAGGAAC AGTTGAAAGA TCCAGTGTGC
    TACCTTAAGA AGGCATTTCT CCTGGTACAA GACATAATGG AGGACACCAT GCGCTTCAGA
    GATAACACCC CCAATGCCAT CGCCATTGTG CAGCTGCAGG AACTCTCTTT GAGGCTGAAG
    AGCTGCTTCA CCAAGGATTA TGAAGAGCAT GACAAGGCCT GCGTCCGAAC TTTCTATGAG
    ACACCTCTCC AGTTGCTGGA GAAGGTCAAG AATGTCTTTA ATGAAACAAA GAATCTCCTT
    GACAAGGACT GGAATATTTT CAGCAAGAAC TGCAACAACA GCTTTGCTGA ATGCTCCAGC
    CAAGATGTGG TGACCAAGCC TGATTGCAAC TGCCTGTACC CCAAAGCCAT CCCTAGCAGT
    GACCCGGCCT CTGTCTCCCC TCATCAGCCC CTCGCCCCCT CCATGGCCCC TGTGGCTGGC
    TTGACCTGGG AGGACTCTGA GGGAACTGAG GGCAGCTCCC TCTTGCCTGG TGAGCAGCCC
    CTGCACACAG TGGATCCAGG CAGTGCCAAG CAGCGGCCAC CCAGGAGCAC CTGCCAGAGC
    TTTGAGCCGC CAGAGACCCC AGTTGTCAAG GACAGCACCA TCGGTGGCTC ACCACAGCCT
    CGCCCCTCTG TCGGGGCCTT CAACCCCGGG ATGGAGGATA TTCTTGACTC TGCAATGGGC
    ACTAATTGGG TCCCAGAAGA AGCCTCTGGA GAGGCCAGTG AGATTCCCGT ACCCCAAGGG
    ACAGAGCTTT CCCCCTCCAG GCCAGGAGGG GGCAGCATGC AGACAGAGCC CGCCAGACCC
    AGCAACTTCC TCTCAGCATC TTCTCCACTC CCTGCATCAG CAAAGGGCCA ACAGCCGGCA
    GATGTAACTG GCCATGAGAG GCAGTCCGAG GGATCCTCCA GCCCGCAGCT CCAGGAGTCT
    GTCTTCCACC TGCTGGTGCC CAGTGTCATC CTGGTCTTGC TGGCCGTCGG AGGCCTCTTG
    TTCTACAGGT GGAGGCGGCG GAGCCATCAA GAGCCTCAGA GAGCGGATTC TCCCTTGGAG
    CAACCAGAGG GCAGCCCCCT GACTCAGGAT GACAGACAGG TGGAACTGCC AGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_757349.1
    CDS414..1730
    Translation

    Target ORF information:

    RefSeq Version NM_172210.2
    Organism Homo sapiens(human)
    Definition Homo sapiens colony stimulating factor 1 (CSF1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_172210.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACCGCGC CGGGCGCCGC CGGGCGCTGC CCTCCCACGA CATGGCTGGG CTCCCTGCTG 
    TTGTTGGTCT GTCTCCTGGC GAGCAGGAGT ATCACCGAGG AGGTGTCGGA GTACTGTAGC
    CACATGATTG GGAGTGGACA CCTGCAGTCT CTGCAGCGGC TGATTGACAG TCAGATGGAG
    ACCTCGTGCC AAATTACATT TGAGTTTGTA GACCAGGAAC AGTTGAAAGA TCCAGTGTGC
    TACCTTAAGA AGGCATTTCT CCTGGTACAA GACATAATGG AGGACACCAT GCGCTTCAGA
    GATAACACCC CCAATGCCAT CGCCATTGTG CAGCTGCAGG AACTCTCTTT GAGGCTGAAG
    AGCTGCTTCA CCAAGGATTA TGAAGAGCAT GACAAGGCCT GCGTCCGAAC TTTCTATGAG
    ACACCTCTCC AGTTGCTGGA GAAGGTCAAG AATGTCTTTA ATGAAACAAA GAATCTCCTT
    GACAAGGACT GGAATATTTT CAGCAAGAAC TGCAACAACA GCTTTGCTGA ATGCTCCAGC
    CAAGATGTGG TGACCAAGCC TGATTGCAAC TGCCTGTACC CCAAAGCCAT CCCTAGCAGT
    GACCCGGCCT CTGTCTCCCC TCATCAGCCC CTCGCCCCCT CCATGGCCCC TGTGGCTGGC
    TTGACCTGGG AGGACTCTGA GGGAACTGAG GGCAGCTCCC TCTTGCCTGG TGAGCAGCCC
    CTGCACACAG TGGATCCAGG CAGTGCCAAG CAGCGGCCAC CCAGGAGCAC CTGCCAGAGC
    TTTGAGCCGC CAGAGACCCC AGTTGTCAAG GACAGCACCA TCGGTGGCTC ACCACAGCCT
    CGCCCCTCTG TCGGGGCCTT CAACCCCGGG ATGGAGGATA TTCTTGACTC TGCAATGGGC
    ACTAATTGGG TCCCAGAAGA AGCCTCTGGA GAGGCCAGTG AGATTCCCGT ACCCCAAGGG
    ACAGAGCTTT CCCCCTCCAG GCCAGGAGGG GGCAGCATGC AGACAGAGCC CGCCAGACCC
    AGCAACTTCC TCTCAGCATC TTCTCCACTC CCTGCATCAG CAAAGGGCCA ACAGCCGGCA
    GATGTAACTG GCCATGAGAG GCAGTCCGAG GGATCCTCCA GCCCGCAGCT CCAGGAGTCT
    GTCTTCCACC TGCTGGTGCC CAGTGTCATC CTGGTCTTGC TGGCCGTCGG AGGCCTCTTG
    TTCTACAGGT GGAGGCGGCG GAGCCATCAA GAGCCTCAGA GAGCGGATTC TCCCTTGGAG
    CAACCAGAGG GCAGCCCCCT GACTCAGGAT GACAGACAGG TGGAACTGCC AGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu11756
    Clone ID Related Accession (Same CDS sequence) NM_172212.2 , NM_000757.5
    Accession Version NM_172212.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1665bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-17
    Organism Homo sapiens(human)
    Product macrophage colony-stimulating factor 1 isoform a precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA414355.1, DB287787.1, M27087.1, BC021117.1 and AL450468.6. On Jan 30, 2008 this sequence version replaced NM_172212.1. Transcript Variant: This variant (4) differs in the 3' UTR compared to variant 1. Both variants 1 and 4 encode isoform a. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. CCDS Note: This CCDS representation uses a downstream in-frame AUG start codon, which is supported by homology and publication data. The use of the 5'-most in-frame AUG in the transcript would result in a 74 aa N-terminal extension, but this N-terminal extension is not conserved in other species, and it would eliminate an N-terminal signal peptide, which is known to be present based on experimental evidence (PMIDs 8262907, 3498652). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC021117.1, M64592.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal (PMID:3500041) ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGACCGCGC CGGGCGCCGC CGGGCGCTGC CCTCCCACGA CATGGCTGGG CTCCCTGCTG 
    TTGTTGGTCT GTCTCCTGGC GAGCAGGAGT ATCACCGAGG AGGTGTCGGA GTACTGTAGC
    CACATGATTG GGAGTGGACA CCTGCAGTCT CTGCAGCGGC TGATTGACAG TCAGATGGAG
    ACCTCGTGCC AAATTACATT TGAGTTTGTA GACCAGGAAC AGTTGAAAGA TCCAGTGTGC
    TACCTTAAGA AGGCATTTCT CCTGGTACAA GACATAATGG AGGACACCAT GCGCTTCAGA
    GATAACACCC CCAATGCCAT CGCCATTGTG CAGCTGCAGG AACTCTCTTT GAGGCTGAAG
    AGCTGCTTCA CCAAGGATTA TGAAGAGCAT GACAAGGCCT GCGTCCGAAC TTTCTATGAG
    ACACCTCTCC AGTTGCTGGA GAAGGTCAAG AATGTCTTTA ATGAAACAAA GAATCTCCTT
    GACAAGGACT GGAATATTTT CAGCAAGAAC TGCAACAACA GCTTTGCTGA ATGCTCCAGC
    CAAGATGTGG TGACCAAGCC TGATTGCAAC TGCCTGTACC CCAAAGCCAT CCCTAGCAGT
    GACCCGGCCT CTGTCTCCCC TCATCAGCCC CTCGCCCCCT CCATGGCCCC TGTGGCTGGC
    TTGACCTGGG AGGACTCTGA GGGAACTGAG GGCAGCTCCC TCTTGCCTGG TGAGCAGCCC
    CTGCACACAG TGGATCCAGG CAGTGCCAAG CAGCGGCCAC CCAGGAGCAC CTGCCAGAGC
    TTTGAGCCGC CAGAGACCCC AGTTGTCAAG GACAGCACCA TCGGTGGCTC ACCACAGCCT
    CGCCCCTCTG TCGGGGCCTT CAACCCCGGG ATGGAGGATA TTCTTGACTC TGCAATGGGC
    ACTAATTGGG TCCCAGAAGA AGCCTCTGGA GAGGCCAGTG AGATTCCCGT ACCCCAAGGG
    ACAGAGCTTT CCCCCTCCAG GCCAGGAGGG GGCAGCATGC AGACAGAGCC CGCCAGACCC
    AGCAACTTCC TCTCAGCATC TTCTCCACTC CCTGCATCAG CAAAGGGCCA ACAGCCGGCA
    GATGTAACTG GTACCGCCTT GCCCAGGGTG GGCCCCGTGA GGCCCACTGG CCAGGACTGG
    AATCACACCC CCCAGAAGAC AGACCATCCA TCTGCCCTGC TCAGAGACCC CCCGGAGCCA
    GGCTCTCCCA GGATCTCATC ACTGCGCCCC CAGGGCCTCA GCAACCCCTC CACCCTCTCT
    GCTCAGCCAC AGCTTTCCAG AAGCCACTCC TCGGGCAGCG TGCTGCCCCT TGGGGAGCTG
    GAGGGCAGGA GGAGCACCAG GGATCGGAGG AGCCCCGCAG AGCCAGAAGG AGGACCAGCA
    AGTGAAGGGG CAGCCAGGCC CCTGCCCCGT TTTAACTCCG TTCCTTTGAC TGACACAGGC
    CATGAGAGGC AGTCCGAGGG ATCCTCCAGC CCGCAGCTCC AGGAGTCTGT CTTCCACCTG
    CTGGTGCCCA GTGTCATCCT GGTCTTGCTG GCCGTCGGAG GCCTCTTGTT CTACAGGTGG
    AGGCGGCGGA GCCATCAAGA GCCTCAGAGA GCGGATTCTC CCTTGGAGCA ACCAGAGGGC
    AGCCCCCTGA CTCAGGATGA CAGACAGGTG GAACTGCCAG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_757351.1
    CDS414..2078
    Translation

    Target ORF information:

    RefSeq Version NM_172212.2
    Organism Homo sapiens(human)
    Definition Homo sapiens colony stimulating factor 1 (CSF1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_172212.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGACCGCGC CGGGCGCCGC CGGGCGCTGC CCTCCCACGA CATGGCTGGG CTCCCTGCTG 
    TTGTTGGTCT GTCTCCTGGC GAGCAGGAGT ATCACCGAGG AGGTGTCGGA GTACTGTAGC
    CACATGATTG GGAGTGGACA CCTGCAGTCT CTGCAGCGGC TGATTGACAG TCAGATGGAG
    ACCTCGTGCC AAATTACATT TGAGTTTGTA GACCAGGAAC AGTTGAAAGA TCCAGTGTGC
    TACCTTAAGA AGGCATTTCT CCTGGTACAA GACATAATGG AGGACACCAT GCGCTTCAGA
    GATAACACCC CCAATGCCAT CGCCATTGTG CAGCTGCAGG AACTCTCTTT GAGGCTGAAG
    AGCTGCTTCA CCAAGGATTA TGAAGAGCAT GACAAGGCCT GCGTCCGAAC TTTCTATGAG
    ACACCTCTCC AGTTGCTGGA GAAGGTCAAG AATGTCTTTA ATGAAACAAA GAATCTCCTT
    GACAAGGACT GGAATATTTT CAGCAAGAAC TGCAACAACA GCTTTGCTGA ATGCTCCAGC
    CAAGATGTGG TGACCAAGCC TGATTGCAAC TGCCTGTACC CCAAAGCCAT CCCTAGCAGT
    GACCCGGCCT CTGTCTCCCC TCATCAGCCC CTCGCCCCCT CCATGGCCCC TGTGGCTGGC
    TTGACCTGGG AGGACTCTGA GGGAACTGAG GGCAGCTCCC TCTTGCCTGG TGAGCAGCCC
    CTGCACACAG TGGATCCAGG CAGTGCCAAG CAGCGGCCAC CCAGGAGCAC CTGCCAGAGC
    TTTGAGCCGC CAGAGACCCC AGTTGTCAAG GACAGCACCA TCGGTGGCTC ACCACAGCCT
    CGCCCCTCTG TCGGGGCCTT CAACCCCGGG ATGGAGGATA TTCTTGACTC TGCAATGGGC
    ACTAATTGGG TCCCAGAAGA AGCCTCTGGA GAGGCCAGTG AGATTCCCGT ACCCCAAGGG
    ACAGAGCTTT CCCCCTCCAG GCCAGGAGGG GGCAGCATGC AGACAGAGCC CGCCAGACCC
    AGCAACTTCC TCTCAGCATC TTCTCCACTC CCTGCATCAG CAAAGGGCCA ACAGCCGGCA
    GATGTAACTG GTACCGCCTT GCCCAGGGTG GGCCCCGTGA GGCCCACTGG CCAGGACTGG
    AATCACACCC CCCAGAAGAC AGACCATCCA TCTGCCCTGC TCAGAGACCC CCCGGAGCCA
    GGCTCTCCCA GGATCTCATC ACTGCGCCCC CAGGGCCTCA GCAACCCCTC CACCCTCTCT
    GCTCAGCCAC AGCTTTCCAG AAGCCACTCC TCGGGCAGCG TGCTGCCCCT TGGGGAGCTG
    GAGGGCAGGA GGAGCACCAG GGATCGGAGG AGCCCCGCAG AGCCAGAAGG AGGACCAGCA
    AGTGAAGGGG CAGCCAGGCC CCTGCCCCGT TTTAACTCCG TTCCTTTGAC TGACACAGGC
    CATGAGAGGC AGTCCGAGGG ATCCTCCAGC CCGCAGCTCC AGGAGTCTGT CTTCCACCTG
    CTGGTGCCCA GTGTCATCCT GGTCTTGCTG GCCGTCGGAG GCCTCTTGTT CTACAGGTGG
    AGGCGGCGGA GCCATCAAGA GCCTCAGAGA GCGGATTCTC CCTTGGAGCA ACCAGAGGGC
    AGCCCCCTGA CTCAGGATGA CAGACAGGTG GAACTGCCAG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu11756
    Clone ID Related Accession (Same CDS sequence) NM_172212.2 , NM_000757.5
    Accession Version NM_000757.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1665bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-27
    Organism Homo sapiens(human)
    Product macrophage colony-stimulating factor 1 isoform a precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA414355.1, BC021117.1, AL450468.6 and AI758702.1. This sequence is a reference standard in the RefSeqGene project. On Sep 20, 2011 this sequence version replaced NM_000757.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: This CCDS representation uses a downstream in-frame AUG start codon, which is supported by homology and publication data. The use of the 5'-most in-frame AUG in the transcript would result in a 74 aa N-terminal extension, but this N-terminal extension is not conserved in other species, and it would eliminate an N-terminal signal peptide, which is known to be present based on experimental evidence (PMIDs 8262907, 3498652). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.39232.1, BC021117.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal (PMID:3500041) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGACCGCGC CGGGCGCCGC CGGGCGCTGC CCTCCCACGA CATGGCTGGG CTCCCTGCTG 
    TTGTTGGTCT GTCTCCTGGC GAGCAGGAGT ATCACCGAGG AGGTGTCGGA GTACTGTAGC
    CACATGATTG GGAGTGGACA CCTGCAGTCT CTGCAGCGGC TGATTGACAG TCAGATGGAG
    ACCTCGTGCC AAATTACATT TGAGTTTGTA GACCAGGAAC AGTTGAAAGA TCCAGTGTGC
    TACCTTAAGA AGGCATTTCT CCTGGTACAA GACATAATGG AGGACACCAT GCGCTTCAGA
    GATAACACCC CCAATGCCAT CGCCATTGTG CAGCTGCAGG AACTCTCTTT GAGGCTGAAG
    AGCTGCTTCA CCAAGGATTA TGAAGAGCAT GACAAGGCCT GCGTCCGAAC TTTCTATGAG
    ACACCTCTCC AGTTGCTGGA GAAGGTCAAG AATGTCTTTA ATGAAACAAA GAATCTCCTT
    GACAAGGACT GGAATATTTT CAGCAAGAAC TGCAACAACA GCTTTGCTGA ATGCTCCAGC
    CAAGATGTGG TGACCAAGCC TGATTGCAAC TGCCTGTACC CCAAAGCCAT CCCTAGCAGT
    GACCCGGCCT CTGTCTCCCC TCATCAGCCC CTCGCCCCCT CCATGGCCCC TGTGGCTGGC
    TTGACCTGGG AGGACTCTGA GGGAACTGAG GGCAGCTCCC TCTTGCCTGG TGAGCAGCCC
    CTGCACACAG TGGATCCAGG CAGTGCCAAG CAGCGGCCAC CCAGGAGCAC CTGCCAGAGC
    TTTGAGCCGC CAGAGACCCC AGTTGTCAAG GACAGCACCA TCGGTGGCTC ACCACAGCCT
    CGCCCCTCTG TCGGGGCCTT CAACCCCGGG ATGGAGGATA TTCTTGACTC TGCAATGGGC
    ACTAATTGGG TCCCAGAAGA AGCCTCTGGA GAGGCCAGTG AGATTCCCGT ACCCCAAGGG
    ACAGAGCTTT CCCCCTCCAG GCCAGGAGGG GGCAGCATGC AGACAGAGCC CGCCAGACCC
    AGCAACTTCC TCTCAGCATC TTCTCCACTC CCTGCATCAG CAAAGGGCCA ACAGCCGGCA
    GATGTAACTG GTACCGCCTT GCCCAGGGTG GGCCCCGTGA GGCCCACTGG CCAGGACTGG
    AATCACACCC CCCAGAAGAC AGACCATCCA TCTGCCCTGC TCAGAGACCC CCCGGAGCCA
    GGCTCTCCCA GGATCTCATC ACTGCGCCCC CAGGGCCTCA GCAACCCCTC CACCCTCTCT
    GCTCAGCCAC AGCTTTCCAG AAGCCACTCC TCGGGCAGCG TGCTGCCCCT TGGGGAGCTG
    GAGGGCAGGA GGAGCACCAG GGATCGGAGG AGCCCCGCAG AGCCAGAAGG AGGACCAGCA
    AGTGAAGGGG CAGCCAGGCC CCTGCCCCGT TTTAACTCCG TTCCTTTGAC TGACACAGGC
    CATGAGAGGC AGTCCGAGGG ATCCTCCAGC CCGCAGCTCC AGGAGTCTGT CTTCCACCTG
    CTGGTGCCCA GTGTCATCCT GGTCTTGCTG GCCGTCGGAG GCCTCTTGTT CTACAGGTGG
    AGGCGGCGGA GCCATCAAGA GCCTCAGAGA GCGGATTCTC CCTTGGAGCA ACCAGAGGGC
    AGCCCCCTGA CTCAGGATGA CAGACAGGTG GAACTGCCAG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000748.3
    CDS414..2078
    Translation

    Target ORF information:

    RefSeq Version NM_000757.5
    Organism Homo sapiens(human)
    Definition Homo sapiens colony stimulating factor 1 (CSF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000757.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGACCGCGC CGGGCGCCGC CGGGCGCTGC CCTCCCACGA CATGGCTGGG CTCCCTGCTG 
    TTGTTGGTCT GTCTCCTGGC GAGCAGGAGT ATCACCGAGG AGGTGTCGGA GTACTGTAGC
    CACATGATTG GGAGTGGACA CCTGCAGTCT CTGCAGCGGC TGATTGACAG TCAGATGGAG
    ACCTCGTGCC AAATTACATT TGAGTTTGTA GACCAGGAAC AGTTGAAAGA TCCAGTGTGC
    TACCTTAAGA AGGCATTTCT CCTGGTACAA GACATAATGG AGGACACCAT GCGCTTCAGA
    GATAACACCC CCAATGCCAT CGCCATTGTG CAGCTGCAGG AACTCTCTTT GAGGCTGAAG
    AGCTGCTTCA CCAAGGATTA TGAAGAGCAT GACAAGGCCT GCGTCCGAAC TTTCTATGAG
    ACACCTCTCC AGTTGCTGGA GAAGGTCAAG AATGTCTTTA ATGAAACAAA GAATCTCCTT
    GACAAGGACT GGAATATTTT CAGCAAGAAC TGCAACAACA GCTTTGCTGA ATGCTCCAGC
    CAAGATGTGG TGACCAAGCC TGATTGCAAC TGCCTGTACC CCAAAGCCAT CCCTAGCAGT
    GACCCGGCCT CTGTCTCCCC TCATCAGCCC CTCGCCCCCT CCATGGCCCC TGTGGCTGGC
    TTGACCTGGG AGGACTCTGA GGGAACTGAG GGCAGCTCCC TCTTGCCTGG TGAGCAGCCC
    CTGCACACAG TGGATCCAGG CAGTGCCAAG CAGCGGCCAC CCAGGAGCAC CTGCCAGAGC
    TTTGAGCCGC CAGAGACCCC AGTTGTCAAG GACAGCACCA TCGGTGGCTC ACCACAGCCT
    CGCCCCTCTG TCGGGGCCTT CAACCCCGGG ATGGAGGATA TTCTTGACTC TGCAATGGGC
    ACTAATTGGG TCCCAGAAGA AGCCTCTGGA GAGGCCAGTG AGATTCCCGT ACCCCAAGGG
    ACAGAGCTTT CCCCCTCCAG GCCAGGAGGG GGCAGCATGC AGACAGAGCC CGCCAGACCC
    AGCAACTTCC TCTCAGCATC TTCTCCACTC CCTGCATCAG CAAAGGGCCA ACAGCCGGCA
    GATGTAACTG GTACCGCCTT GCCCAGGGTG GGCCCCGTGA GGCCCACTGG CCAGGACTGG
    AATCACACCC CCCAGAAGAC AGACCATCCA TCTGCCCTGC TCAGAGACCC CCCGGAGCCA
    GGCTCTCCCA GGATCTCATC ACTGCGCCCC CAGGGCCTCA GCAACCCCTC CACCCTCTCT
    GCTCAGCCAC AGCTTTCCAG AAGCCACTCC TCGGGCAGCG TGCTGCCCCT TGGGGAGCTG
    GAGGGCAGGA GGAGCACCAG GGATCGGAGG AGCCCCGCAG AGCCAGAAGG AGGACCAGCA
    AGTGAAGGGG CAGCCAGGCC CCTGCCCCGT TTTAACTCCG TTCCTTTGAC TGACACAGGC
    CATGAGAGGC AGTCCGAGGG ATCCTCCAGC CCGCAGCTCC AGGAGTCTGT CTTCCACCTG
    CTGGTGCCCA GTGTCATCCT GGTCTTGCTG GCCGTCGGAG GCCTCTTGTT CTACAGGTGG
    AGGCGGCGGA GCCATCAAGA GCCTCAGAGA GCGGATTCTC CCTTGGAGCA ACCAGAGGGC
    AGCCCCCTGA CTCAGGATGA CAGACAGGTG GAACTGCCAG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12311
    Clone ID Related Accession (Same CDS sequence) NM_172211.3
    Accession Version NM_172211.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 771bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product macrophage colony-stimulating factor 1 isoform c precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA414355.1, BC021117.1, M76453.1, AL450468.6 and AI758702.1. On Apr 18, 2012 this sequence version replaced NM_172211.2. Transcript Variant: This variant (3) lacks an alternate in-frame segment compared to variant 1, resulting in a shorter isoform (c) compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal (PMID:3500041) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGACCGCGC CGGGCGCCGC CGGGCGCTGC CCTCCCACGA CATGGCTGGG CTCCCTGCTG 
    TTGTTGGTCT GTCTCCTGGC GAGCAGGAGT ATCACCGAGG AGGTGTCGGA GTACTGTAGC
    CACATGATTG GGAGTGGACA CCTGCAGTCT CTGCAGCGGC TGATTGACAG TCAGATGGAG
    ACCTCGTGCC AAATTACATT TGAGTTTGTA GACCAGGAAC AGTTGAAAGA TCCAGTGTGC
    TACCTTAAGA AGGCATTTCT CCTGGTACAA GACATAATGG AGGACACCAT GCGCTTCAGA
    GATAACACCC CCAATGCCAT CGCCATTGTG CAGCTGCAGG AACTCTCTTT GAGGCTGAAG
    AGCTGCTTCA CCAAGGATTA TGAAGAGCAT GACAAGGCCT GCGTCCGAAC TTTCTATGAG
    ACACCTCTCC AGTTGCTGGA GAAGGTCAAG AATGTCTTTA ATGAAACAAA GAATCTCCTT
    GACAAGGACT GGAATATTTT CAGCAAGAAC TGCAACAACA GCTTTGCTGA ATGCTCCAGC
    CAAGGCCATG AGAGGCAGTC CGAGGGATCC TCCAGCCCGC AGCTCCAGGA GTCTGTCTTC
    CACCTGCTGG TGCCCAGTGT CATCCTGGTC TTGCTGGCCG TCGGAGGCCT CTTGTTCTAC
    AGGTGGAGGC GGCGGAGCCA TCAAGAGCCT CAGAGAGCGG ATTCTCCCTT GGAGCAACCA
    GAGGGCAGCC CCCTGACTCA GGATGACAGA CAGGTGGAAC TGCCAGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_757350.1
    CDS414..1184
    Translation

    Target ORF information:

    RefSeq Version NM_172211.3
    Organism Homo sapiens(human)
    Definition Homo sapiens colony stimulating factor 1 (CSF1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_172211.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGACCGCGC CGGGCGCCGC CGGGCGCTGC CCTCCCACGA CATGGCTGGG CTCCCTGCTG 
    TTGTTGGTCT GTCTCCTGGC GAGCAGGAGT ATCACCGAGG AGGTGTCGGA GTACTGTAGC
    CACATGATTG GGAGTGGACA CCTGCAGTCT CTGCAGCGGC TGATTGACAG TCAGATGGAG
    ACCTCGTGCC AAATTACATT TGAGTTTGTA GACCAGGAAC AGTTGAAAGA TCCAGTGTGC
    TACCTTAAGA AGGCATTTCT CCTGGTACAA GACATAATGG AGGACACCAT GCGCTTCAGA
    GATAACACCC CCAATGCCAT CGCCATTGTG CAGCTGCAGG AACTCTCTTT GAGGCTGAAG
    AGCTGCTTCA CCAAGGATTA TGAAGAGCAT GACAAGGCCT GCGTCCGAAC TTTCTATGAG
    ACACCTCTCC AGTTGCTGGA GAAGGTCAAG AATGTCTTTA ATGAAACAAA GAATCTCCTT
    GACAAGGACT GGAATATTTT CAGCAAGAAC TGCAACAACA GCTTTGCTGA ATGCTCCAGC
    CAAGGCCATG AGAGGCAGTC CGAGGGATCC TCCAGCCCGC AGCTCCAGGA GTCTGTCTTC
    CACCTGCTGG TGCCCAGTGT CATCCTGGTC TTGCTGGCCG TCGGAGGCCT CTTGTTCTAC
    AGGTGGAGGC GGCGGAGCCA TCAAGAGCCT CAGAGAGCGG ATTCTCCCTT GGAGCAACCA
    GAGGGCAGCC CCCTGACTCA GGATGACAGA CAGGTGGAAC TGCCAGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108521
    Clone ID Related Accession (Same CDS sequence) NM_172211.4
    Accession Version NM_172211.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 771bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-02
    Organism Homo sapiens(human)
    Product macrophage colony-stimulating factor 1 isoform c precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL450468.6. On Jun 3, 2019 this sequence version replaced NM_172211.3. Transcript Variant: This variant (3) lacks an alternate in-frame segment compared to variant 1, resulting in a shorter isoform (c) compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DRR138518.291639.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGACCGCGC CGGGCGCCGC CGGGCGCTGC CCTCCCACGA CATGGCTGGG CTCCCTGCTG 
    TTGTTGGTCT GTCTCCTGGC GAGCAGGAGT ATCACCGAGG AGGTGTCGGA GTACTGTAGC
    CACATGATTG GGAGTGGACA CCTGCAGTCT CTGCAGCGGC TGATTGACAG TCAGATGGAG
    ACCTCGTGCC AAATTACATT TGAGTTTGTA GACCAGGAAC AGTTGAAAGA TCCAGTGTGC
    TACCTTAAGA AGGCATTTCT CCTGGTACAA GACATAATGG AGGACACCAT GCGCTTCAGA
    GATAACACCC CCAATGCCAT CGCCATTGTG CAGCTGCAGG AACTCTCTTT GAGGCTGAAG
    AGCTGCTTCA CCAAGGATTA TGAAGAGCAT GACAAGGCCT GCGTCCGAAC TTTCTATGAG
    ACACCTCTCC AGTTGCTGGA GAAGGTCAAG AATGTCTTTA ATGAAACAAA GAATCTCCTT
    GACAAGGACT GGAATATTTT CAGCAAGAAC TGCAACAACA GCTTTGCTGA ATGCTCCAGC
    CAAGGCCATG AGAGGCAGTC CGAGGGATCC TTCAGCCCGC AGCTCCAGGA GTCTGTCTTC
    CACCTGCTGG TGCCCAGTGT CATCCTGGTC TTGCTGGCCG TCGGAGGCCT CTTGTTCTAC
    AGGTGGAGGC GGCGGAGCCA TCAAGAGCCT CAGAGAGCGG ATTCTCCCTT GGAGCAACCA
    GAGGGCAGCC CCCTGACTCA GGATGACAGA CAGGTGGAAC TGCCAGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_757350.2
    CDS176..946
    Translation

    Target ORF information:

    RefSeq Version NM_172211.4
    Organism Homo sapiens(human)
    Definition Homo sapiens colony stimulating factor 1 (CSF1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_172211.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGACCGCGC CGGGCGCCGC CGGGCGCTGC CCTCCCACGA CATGGCTGGG CTCCCTGCTG 
    TTGTTGGTCT GTCTCCTGGC GAGCAGGAGT ATCACCGAGG AGGTGTCGGA GTACTGTAGC
    CACATGATTG GGAGTGGACA CCTGCAGTCT CTGCAGCGGC TGATTGACAG TCAGATGGAG
    ACCTCGTGCC AAATTACATT TGAGTTTGTA GACCAGGAAC AGTTGAAAGA TCCAGTGTGC
    TACCTTAAGA AGGCATTTCT CCTGGTACAA GACATAATGG AGGACACCAT GCGCTTCAGA
    GATAACACCC CCAATGCCAT CGCCATTGTG CAGCTGCAGG AACTCTCTTT GAGGCTGAAG
    AGCTGCTTCA CCAAGGATTA TGAAGAGCAT GACAAGGCCT GCGTCCGAAC TTTCTATGAG
    ACACCTCTCC AGTTGCTGGA GAAGGTCAAG AATGTCTTTA ATGAAACAAA GAATCTCCTT
    GACAAGGACT GGAATATTTT CAGCAAGAAC TGCAACAACA GCTTTGCTGA ATGCTCCAGC
    CAAGGCCATG AGAGGCAGTC CGAGGGATCC TTCAGCCCGC AGCTCCAGGA GTCTGTCTTC
    CACCTGCTGG TGCCCAGTGT CATCCTGGTC TTGCTGGCCG TCGGAGGCCT CTTGTTCTAC
    AGGTGGAGGC GGCGGAGCCA TCAAGAGCCT CAGAGAGCGG ATTCTCCCTT GGAGCAACCA
    GAGGGCAGCC CCCTGACTCA GGATGACAGA CAGGTGGAAC TGCCAGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108673
    Clone ID Related Accession (Same CDS sequence) NM_172210.3
    Accession Version NM_172210.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1317bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-02
    Organism Homo sapiens(human)
    Product macrophage colony-stimulating factor 1 isoform b precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL450468.6. On Jun 3, 2019 this sequence version replaced NM_172210.2. Transcript Variant: This variant (2) lacks an alternate in-frame segment compared to variant 1, resulting in a shorter isoform (b) compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U22386.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACCGCGC CGGGCGCCGC CGGGCGCTGC CCTCCCACGA CATGGCTGGG CTCCCTGCTG 
    TTGTTGGTCT GTCTCCTGGC GAGCAGGAGT ATCACCGAGG AGGTGTCGGA GTACTGTAGC
    CACATGATTG GGAGTGGACA CCTGCAGTCT CTGCAGCGGC TGATTGACAG TCAGATGGAG
    ACCTCGTGCC AAATTACATT TGAGTTTGTA GACCAGGAAC AGTTGAAAGA TCCAGTGTGC
    TACCTTAAGA AGGCATTTCT CCTGGTACAA GACATAATGG AGGACACCAT GCGCTTCAGA
    GATAACACCC CCAATGCCAT CGCCATTGTG CAGCTGCAGG AACTCTCTTT GAGGCTGAAG
    AGCTGCTTCA CCAAGGATTA TGAAGAGCAT GACAAGGCCT GCGTCCGAAC TTTCTATGAG
    ACACCTCTCC AGTTGCTGGA GAAGGTCAAG AATGTCTTTA ATGAAACAAA GAATCTCCTT
    GACAAGGACT GGAATATTTT CAGCAAGAAC TGCAACAACA GCTTTGCTGA ATGCTCCAGC
    CAAGATGTGG TGACCAAGCC TGATTGCAAC TGCCTGTACC CCAAAGCCAT CCCTAGCAGT
    GACCCGGCCT CTGTCTCCCC TCATCAGCCC CTCGCCCCCT CCATGGCCCC TGTGGCTGGC
    TTGACCTGGG AGGACTCTGA GGGAACTGAG GGCAGCTCCC TCTTGCCTGG TGAGCAGCCC
    CTGCACACAG TGGATCCAGG CAGTGCCAAG CAGCGGCCAC CCAGGAGCAC CTGCCAGAGC
    TTTGAGCCGC CAGAGACCCC AGTTGTCAAG GACAGCACCA TCGGTGGCTC ACCACAGCCT
    CGCCCCTCTG TCGGGGCCTT CAACCCCGGG ATGGAGGATA TTCTTGACTC TGCAATGGGC
    ACTAATTGGG TCCCAGAAGA AGCCTCTGGA GAGGCCAGTG AGATTCCCGT ACCCCAAGGG
    ACAGAGCTTT CCCCCTCCAG GCCAGGAGGG GGCAGCATGC AGACAGAGCC CGCCAGACCC
    AGCAACTTCC TCTCAGCATC TTCTCCACTC CCTGCATCAG CAAAGGGCCA ACAGCCGGCA
    GATGTAACTG GCCATGAGAG GCAGTCCGAG GGATCCTTCA GCCCGCAGCT CCAGGAGTCT
    GTCTTCCACC TGCTGGTGCC CAGTGTCATC CTGGTCTTGC TGGCCGTCGG AGGCCTCTTG
    TTCTACAGGT GGAGGCGGCG GAGCCATCAA GAGCCTCAGA GAGCGGATTC TCCCTTGGAG
    CAACCAGAGG GCAGCCCCCT GACTCAGGAT GACAGACAGG TGGAACTGCC AGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_757349.2
    CDS176..1492
    Translation

    Target ORF information:

    RefSeq Version NM_172210.3
    Organism Homo sapiens(human)
    Definition Homo sapiens colony stimulating factor 1 (CSF1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_172210.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACCGCGC CGGGCGCCGC CGGGCGCTGC CCTCCCACGA CATGGCTGGG CTCCCTGCTG 
    TTGTTGGTCT GTCTCCTGGC GAGCAGGAGT ATCACCGAGG AGGTGTCGGA GTACTGTAGC
    CACATGATTG GGAGTGGACA CCTGCAGTCT CTGCAGCGGC TGATTGACAG TCAGATGGAG
    ACCTCGTGCC AAATTACATT TGAGTTTGTA GACCAGGAAC AGTTGAAAGA TCCAGTGTGC
    TACCTTAAGA AGGCATTTCT CCTGGTACAA GACATAATGG AGGACACCAT GCGCTTCAGA
    GATAACACCC CCAATGCCAT CGCCATTGTG CAGCTGCAGG AACTCTCTTT GAGGCTGAAG
    AGCTGCTTCA CCAAGGATTA TGAAGAGCAT GACAAGGCCT GCGTCCGAAC TTTCTATGAG
    ACACCTCTCC AGTTGCTGGA GAAGGTCAAG AATGTCTTTA ATGAAACAAA GAATCTCCTT
    GACAAGGACT GGAATATTTT CAGCAAGAAC TGCAACAACA GCTTTGCTGA ATGCTCCAGC
    CAAGATGTGG TGACCAAGCC TGATTGCAAC TGCCTGTACC CCAAAGCCAT CCCTAGCAGT
    GACCCGGCCT CTGTCTCCCC TCATCAGCCC CTCGCCCCCT CCATGGCCCC TGTGGCTGGC
    TTGACCTGGG AGGACTCTGA GGGAACTGAG GGCAGCTCCC TCTTGCCTGG TGAGCAGCCC
    CTGCACACAG TGGATCCAGG CAGTGCCAAG CAGCGGCCAC CCAGGAGCAC CTGCCAGAGC
    TTTGAGCCGC CAGAGACCCC AGTTGTCAAG GACAGCACCA TCGGTGGCTC ACCACAGCCT
    CGCCCCTCTG TCGGGGCCTT CAACCCCGGG ATGGAGGATA TTCTTGACTC TGCAATGGGC
    ACTAATTGGG TCCCAGAAGA AGCCTCTGGA GAGGCCAGTG AGATTCCCGT ACCCCAAGGG
    ACAGAGCTTT CCCCCTCCAG GCCAGGAGGG GGCAGCATGC AGACAGAGCC CGCCAGACCC
    AGCAACTTCC TCTCAGCATC TTCTCCACTC CCTGCATCAG CAAAGGGCCA ACAGCCGGCA
    GATGTAACTG GCCATGAGAG GCAGTCCGAG GGATCCTTCA GCCCGCAGCT CCAGGAGTCT
    GTCTTCCACC TGCTGGTGCC CAGTGTCATC CTGGTCTTGC TGGCCGTCGG AGGCCTCTTG
    TTCTACAGGT GGAGGCGGCG GAGCCATCAA GAGCCTCAGA GAGCGGATTC TCCCTTGGAG
    CAACCAGAGG GCAGCCCCCT GACTCAGGAT GACAGACAGG TGGAACTGCC AGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu107858
    Clone ID Related Accession (Same CDS sequence) NM_000757.6 , NM_172212.3
    Accession Version NM_000757.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1665bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-02
    Organism Homo sapiens(human)
    Product macrophage colony-stimulating factor 1 isoform a precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL450468.6. On Nov 24, 2018 this sequence version replaced NM_000757.5. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: This CCDS representation uses a downstream in-frame AUG start codon, which is supported by homology and publication data. The use of the 5'-most in-frame AUG in the transcript would result in a 74 aa N-terminal extension, but this N-terminal extension is not conserved in other species, and it would eliminate an N-terminal signal peptide, which is known to be present based on experimental evidence (PMIDs 8262907, 3498652). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.39232.1, BC021117.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000329608.11/ ENSP00000327513.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGACCGCGC CGGGCGCCGC CGGGCGCTGC CCTCCCACGA CATGGCTGGG CTCCCTGCTG 
    TTGTTGGTCT GTCTCCTGGC GAGCAGGAGT ATCACCGAGG AGGTGTCGGA GTACTGTAGC
    CACATGATTG GGAGTGGACA CCTGCAGTCT CTGCAGCGGC TGATTGACAG TCAGATGGAG
    ACCTCGTGCC AAATTACATT TGAGTTTGTA GACCAGGAAC AGTTGAAAGA TCCAGTGTGC
    TACCTTAAGA AGGCATTTCT CCTGGTACAA GACATAATGG AGGACACCAT GCGCTTCAGA
    GATAACACCC CCAATGCCAT CGCCATTGTG CAGCTGCAGG AACTCTCTTT GAGGCTGAAG
    AGCTGCTTCA CCAAGGATTA TGAAGAGCAT GACAAGGCCT GCGTCCGAAC TTTCTATGAG
    ACACCTCTCC AGTTGCTGGA GAAGGTCAAG AATGTCTTTA ATGAAACAAA GAATCTCCTT
    GACAAGGACT GGAATATTTT CAGCAAGAAC TGCAACAACA GCTTTGCTGA ATGCTCCAGC
    CAAGATGTGG TGACCAAGCC TGATTGCAAC TGCCTGTACC CCAAAGCCAT CCCTAGCAGT
    GACCCGGCCT CTGTCTCCCC TCATCAGCCC CTCGCCCCCT CCATGGCCCC TGTGGCTGGC
    TTGACCTGGG AGGACTCTGA GGGAACTGAG GGCAGCTCCC TCTTGCCTGG TGAGCAGCCC
    CTGCACACAG TGGATCCAGG CAGTGCCAAG CAGCGGCCAC CCAGGAGCAC CTGCCAGAGC
    TTTGAGCCGC CAGAGACCCC AGTTGTCAAG GACAGCACCA TCGGTGGCTC ACCACAGCCT
    CGCCCCTCTG TCGGGGCCTT CAACCCCGGG ATGGAGGATA TTCTTGACTC TGCAATGGGC
    ACTAATTGGG TCCCAGAAGA AGCCTCTGGA GAGGCCAGTG AGATTCCCGT ACCCCAAGGG
    ACAGAGCTTT CCCCCTCCAG GCCAGGAGGG GGCAGCATGC AGACAGAGCC CGCCAGACCC
    AGCAACTTCC TCTCAGCATC TTCTCCACTC CCTGCATCAG CAAAGGGCCA ACAGCCGGCA
    GATGTAACTG GTACCGCCTT GCCCAGGGTG GGCCCCGTGA GGCCCACTGG CCAGGACTGG
    AATCACACCC CCCAGAAGAC AGACCATCCA TCTGCCCTGC TCAGAGACCC CCCGGAGCCA
    GGCTCTCCCA GGATCTCATC ACTGCGCCCC CAGGGCCTCA GCAACCCCTC CACCCTCTCT
    GCTCAGCCAC AGCTTTCCAG AAGCCACTCC TCGGGCAGCG TGCTGCCCCT TGGGGAGCTG
    GAGGGCAGGA GGAGCACCAG GGATCGGAGG AGCCCCGCAG AGCCAGAAGG AGGACCAGCA
    AGTGAAGGGG CAGCCAGGCC CCTGCCCCGT TTTAACTCCG TTCCTTTGAC TGACACAGGC
    CATGAGAGGC AGTCCGAGGG ATCCTTCAGC CCGCAGCTCC AGGAGTCTGT CTTCCACCTG
    CTGGTGCCCA GTGTCATCCT GGTCTTGCTG GCCGTCGGAG GCCTCTTGTT CTACAGGTGG
    AGGCGGCGGA GCCATCAAGA GCCTCAGAGA GCGGATTCTC CCTTGGAGCA ACCAGAGGGC
    AGCCCCCTGA CTCAGGATGA CAGACAGGTG GAACTGCCAG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000748.4
    CDS176..1840
    Translation

    Target ORF information:

    RefSeq Version NM_000757.6
    Organism Homo sapiens(human)
    Definition Homo sapiens colony stimulating factor 1 (CSF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000757.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGACCGCGC CGGGCGCCGC CGGGCGCTGC CCTCCCACGA CATGGCTGGG CTCCCTGCTG 
    TTGTTGGTCT GTCTCCTGGC GAGCAGGAGT ATCACCGAGG AGGTGTCGGA GTACTGTAGC
    CACATGATTG GGAGTGGACA CCTGCAGTCT CTGCAGCGGC TGATTGACAG TCAGATGGAG
    ACCTCGTGCC AAATTACATT TGAGTTTGTA GACCAGGAAC AGTTGAAAGA TCCAGTGTGC
    TACCTTAAGA AGGCATTTCT CCTGGTACAA GACATAATGG AGGACACCAT GCGCTTCAGA
    GATAACACCC CCAATGCCAT CGCCATTGTG CAGCTGCAGG AACTCTCTTT GAGGCTGAAG
    AGCTGCTTCA CCAAGGATTA TGAAGAGCAT GACAAGGCCT GCGTCCGAAC TTTCTATGAG
    ACACCTCTCC AGTTGCTGGA GAAGGTCAAG AATGTCTTTA ATGAAACAAA GAATCTCCTT
    GACAAGGACT GGAATATTTT CAGCAAGAAC TGCAACAACA GCTTTGCTGA ATGCTCCAGC
    CAAGATGTGG TGACCAAGCC TGATTGCAAC TGCCTGTACC CCAAAGCCAT CCCTAGCAGT
    GACCCGGCCT CTGTCTCCCC TCATCAGCCC CTCGCCCCCT CCATGGCCCC TGTGGCTGGC
    TTGACCTGGG AGGACTCTGA GGGAACTGAG GGCAGCTCCC TCTTGCCTGG TGAGCAGCCC
    CTGCACACAG TGGATCCAGG CAGTGCCAAG CAGCGGCCAC CCAGGAGCAC CTGCCAGAGC
    TTTGAGCCGC CAGAGACCCC AGTTGTCAAG GACAGCACCA TCGGTGGCTC ACCACAGCCT
    CGCCCCTCTG TCGGGGCCTT CAACCCCGGG ATGGAGGATA TTCTTGACTC TGCAATGGGC
    ACTAATTGGG TCCCAGAAGA AGCCTCTGGA GAGGCCAGTG AGATTCCCGT ACCCCAAGGG
    ACAGAGCTTT CCCCCTCCAG GCCAGGAGGG GGCAGCATGC AGACAGAGCC CGCCAGACCC
    AGCAACTTCC TCTCAGCATC TTCTCCACTC CCTGCATCAG CAAAGGGCCA ACAGCCGGCA
    GATGTAACTG GTACCGCCTT GCCCAGGGTG GGCCCCGTGA GGCCCACTGG CCAGGACTGG
    AATCACACCC CCCAGAAGAC AGACCATCCA TCTGCCCTGC TCAGAGACCC CCCGGAGCCA
    GGCTCTCCCA GGATCTCATC ACTGCGCCCC CAGGGCCTCA GCAACCCCTC CACCCTCTCT
    GCTCAGCCAC AGCTTTCCAG AAGCCACTCC TCGGGCAGCG TGCTGCCCCT TGGGGAGCTG
    GAGGGCAGGA GGAGCACCAG GGATCGGAGG AGCCCCGCAG AGCCAGAAGG AGGACCAGCA
    AGTGAAGGGG CAGCCAGGCC CCTGCCCCGT TTTAACTCCG TTCCTTTGAC TGACACAGGC
    CATGAGAGGC AGTCCGAGGG ATCCTTCAGC CCGCAGCTCC AGGAGTCTGT CTTCCACCTG
    CTGGTGCCCA GTGTCATCCT GGTCTTGCTG GCCGTCGGAG GCCTCTTGTT CTACAGGTGG
    AGGCGGCGGA GCCATCAAGA GCCTCAGAGA GCGGATTCTC CCTTGGAGCA ACCAGAGGGC
    AGCCCCCTGA CTCAGGATGA CAGACAGGTG GAACTGCCAG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu107858
    Clone ID Related Accession (Same CDS sequence) NM_000757.6 , NM_172212.3
    Accession Version NM_172212.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1665bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-02
    Organism Homo sapiens(human)
    Product macrophage colony-stimulating factor 1 isoform a precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL450468.6. On Jun 3, 2019 this sequence version replaced NM_172212.2. Transcript Variant: This variant (4) differs in the 3' UTR compared to variant 1. Both variants 1 and 4 encode isoform a. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. CCDS Note: This CCDS representation uses a downstream in-frame AUG start codon, which is supported by homology and publication data. The use of the 5'-most in-frame AUG in the transcript would result in a 74 aa N-terminal extension, but this N-terminal extension is not conserved in other species, and it would eliminate an N-terminal signal peptide, which is known to be present based on experimental evidence (PMIDs 8262907, 3498652). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DRR138524.466396.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGACCGCGC CGGGCGCCGC CGGGCGCTGC CCTCCCACGA CATGGCTGGG CTCCCTGCTG 
    TTGTTGGTCT GTCTCCTGGC GAGCAGGAGT ATCACCGAGG AGGTGTCGGA GTACTGTAGC
    CACATGATTG GGAGTGGACA CCTGCAGTCT CTGCAGCGGC TGATTGACAG TCAGATGGAG
    ACCTCGTGCC AAATTACATT TGAGTTTGTA GACCAGGAAC AGTTGAAAGA TCCAGTGTGC
    TACCTTAAGA AGGCATTTCT CCTGGTACAA GACATAATGG AGGACACCAT GCGCTTCAGA
    GATAACACCC CCAATGCCAT CGCCATTGTG CAGCTGCAGG AACTCTCTTT GAGGCTGAAG
    AGCTGCTTCA CCAAGGATTA TGAAGAGCAT GACAAGGCCT GCGTCCGAAC TTTCTATGAG
    ACACCTCTCC AGTTGCTGGA GAAGGTCAAG AATGTCTTTA ATGAAACAAA GAATCTCCTT
    GACAAGGACT GGAATATTTT CAGCAAGAAC TGCAACAACA GCTTTGCTGA ATGCTCCAGC
    CAAGATGTGG TGACCAAGCC TGATTGCAAC TGCCTGTACC CCAAAGCCAT CCCTAGCAGT
    GACCCGGCCT CTGTCTCCCC TCATCAGCCC CTCGCCCCCT CCATGGCCCC TGTGGCTGGC
    TTGACCTGGG AGGACTCTGA GGGAACTGAG GGCAGCTCCC TCTTGCCTGG TGAGCAGCCC
    CTGCACACAG TGGATCCAGG CAGTGCCAAG CAGCGGCCAC CCAGGAGCAC CTGCCAGAGC
    TTTGAGCCGC CAGAGACCCC AGTTGTCAAG GACAGCACCA TCGGTGGCTC ACCACAGCCT
    CGCCCCTCTG TCGGGGCCTT CAACCCCGGG ATGGAGGATA TTCTTGACTC TGCAATGGGC
    ACTAATTGGG TCCCAGAAGA AGCCTCTGGA GAGGCCAGTG AGATTCCCGT ACCCCAAGGG
    ACAGAGCTTT CCCCCTCCAG GCCAGGAGGG GGCAGCATGC AGACAGAGCC CGCCAGACCC
    AGCAACTTCC TCTCAGCATC TTCTCCACTC CCTGCATCAG CAAAGGGCCA ACAGCCGGCA
    GATGTAACTG GTACCGCCTT GCCCAGGGTG GGCCCCGTGA GGCCCACTGG CCAGGACTGG
    AATCACACCC CCCAGAAGAC AGACCATCCA TCTGCCCTGC TCAGAGACCC CCCGGAGCCA
    GGCTCTCCCA GGATCTCATC ACTGCGCCCC CAGGGCCTCA GCAACCCCTC CACCCTCTCT
    GCTCAGCCAC AGCTTTCCAG AAGCCACTCC TCGGGCAGCG TGCTGCCCCT TGGGGAGCTG
    GAGGGCAGGA GGAGCACCAG GGATCGGAGG AGCCCCGCAG AGCCAGAAGG AGGACCAGCA
    AGTGAAGGGG CAGCCAGGCC CCTGCCCCGT TTTAACTCCG TTCCTTTGAC TGACACAGGC
    CATGAGAGGC AGTCCGAGGG ATCCTTCAGC CCGCAGCTCC AGGAGTCTGT CTTCCACCTG
    CTGGTGCCCA GTGTCATCCT GGTCTTGCTG GCCGTCGGAG GCCTCTTGTT CTACAGGTGG
    AGGCGGCGGA GCCATCAAGA GCCTCAGAGA GCGGATTCTC CCTTGGAGCA ACCAGAGGGC
    AGCCCCCTGA CTCAGGATGA CAGACAGGTG GAACTGCCAG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_757351.2
    CDS176..1840
    Translation

    Target ORF information:

    RefSeq Version NM_172212.3
    Organism Homo sapiens(human)
    Definition Homo sapiens colony stimulating factor 1 (CSF1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_172212.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGACCGCGC CGGGCGCCGC CGGGCGCTGC CCTCCCACGA CATGGCTGGG CTCCCTGCTG 
    TTGTTGGTCT GTCTCCTGGC GAGCAGGAGT ATCACCGAGG AGGTGTCGGA GTACTGTAGC
    CACATGATTG GGAGTGGACA CCTGCAGTCT CTGCAGCGGC TGATTGACAG TCAGATGGAG
    ACCTCGTGCC AAATTACATT TGAGTTTGTA GACCAGGAAC AGTTGAAAGA TCCAGTGTGC
    TACCTTAAGA AGGCATTTCT CCTGGTACAA GACATAATGG AGGACACCAT GCGCTTCAGA
    GATAACACCC CCAATGCCAT CGCCATTGTG CAGCTGCAGG AACTCTCTTT GAGGCTGAAG
    AGCTGCTTCA CCAAGGATTA TGAAGAGCAT GACAAGGCCT GCGTCCGAAC TTTCTATGAG
    ACACCTCTCC AGTTGCTGGA GAAGGTCAAG AATGTCTTTA ATGAAACAAA GAATCTCCTT
    GACAAGGACT GGAATATTTT CAGCAAGAAC TGCAACAACA GCTTTGCTGA ATGCTCCAGC
    CAAGATGTGG TGACCAAGCC TGATTGCAAC TGCCTGTACC CCAAAGCCAT CCCTAGCAGT
    GACCCGGCCT CTGTCTCCCC TCATCAGCCC CTCGCCCCCT CCATGGCCCC TGTGGCTGGC
    TTGACCTGGG AGGACTCTGA GGGAACTGAG GGCAGCTCCC TCTTGCCTGG TGAGCAGCCC
    CTGCACACAG TGGATCCAGG CAGTGCCAAG CAGCGGCCAC CCAGGAGCAC CTGCCAGAGC
    TTTGAGCCGC CAGAGACCCC AGTTGTCAAG GACAGCACCA TCGGTGGCTC ACCACAGCCT
    CGCCCCTCTG TCGGGGCCTT CAACCCCGGG ATGGAGGATA TTCTTGACTC TGCAATGGGC
    ACTAATTGGG TCCCAGAAGA AGCCTCTGGA GAGGCCAGTG AGATTCCCGT ACCCCAAGGG
    ACAGAGCTTT CCCCCTCCAG GCCAGGAGGG GGCAGCATGC AGACAGAGCC CGCCAGACCC
    AGCAACTTCC TCTCAGCATC TTCTCCACTC CCTGCATCAG CAAAGGGCCA ACAGCCGGCA
    GATGTAACTG GTACCGCCTT GCCCAGGGTG GGCCCCGTGA GGCCCACTGG CCAGGACTGG
    AATCACACCC CCCAGAAGAC AGACCATCCA TCTGCCCTGC TCAGAGACCC CCCGGAGCCA
    GGCTCTCCCA GGATCTCATC ACTGCGCCCC CAGGGCCTCA GCAACCCCTC CACCCTCTCT
    GCTCAGCCAC AGCTTTCCAG AAGCCACTCC TCGGGCAGCG TGCTGCCCCT TGGGGAGCTG
    GAGGGCAGGA GGAGCACCAG GGATCGGAGG AGCCCCGCAG AGCCAGAAGG AGGACCAGCA
    AGTGAAGGGG CAGCCAGGCC CCTGCCCCGT TTTAACTCCG TTCCTTTGAC TGACACAGGC
    CATGAGAGGC AGTCCGAGGG ATCCTTCAGC CCGCAGCTCC AGGAGTCTGT CTTCCACCTG
    CTGGTGCCCA GTGTCATCCT GGTCTTGCTG GCCGTCGGAG GCCTCTTGTT CTACAGGTGG
    AGGCGGCGGA GCCATCAAGA GCCTCAGAGA GCGGATTCTC CCTTGGAGCA ACCAGAGGGC
    AGCCCCCTGA CTCAGGATGA CAGACAGGTG GAACTGCCAG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu83176
    Clone ID Related Accession (Same CDS sequence) XM_017000369.1
    Accession Version XM_017000369.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product macrophage colony-stimulating factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGATTGGGA GTGGACACCT GCAGTCTCTG CAGCGGCTGA TTGACAGTCA GATGGAGACC 
    TCGTGCCAAA TTACATTTGA GTTTGTAGAC CAGGAACAGT TGAAAGATCC AGTGTGCTAC
    CTTAAGAAGG CATTTCTCCT GGTACAAGAC ATAATGGAGG ACACCATGCG CTTCAGAGAT
    AACACCCCCA ATGCCATCGC CATTGTGCAG CTGCAGGAAC TCTCTTTGAG GCTGAAGAGC
    TGCTTCACCA AGGATTATGA AGAGCATGAC AAGGCCTGCG TCCGAACTTT CTATGAGACA
    CCTCTCCAGT TGCTGGAGAA GGTCAAGAAT GTCTTTAATG AAACAAAGAA TCTCCTTGAC
    AAGGACTGGA ATATTTTCAG CAAGAACTGC AACAACAGCT TTGCTGAATG CTCCAGCCAA
    GATGTGGTGA CCAAGCCTGA TTGCAACTGC CTGTACCCCA AAGCCATCCC TAGCAGTGAC
    CCGGCCTCTG TCTCCCCTCA TCAGCCCCTC GCCCCCTCCA TGGCCCCTGT GGCTGGCTTG
    ACCTGGGAGG ACTCTGAGGG AACTGAGGGC AGCTCCCTCT TGCCTGGTGA GCAGCCCCTG
    CACACAGTGG ATCCAGGCAG TGCCAAGCAG CGGCCACCCA GGAGCACCTG CCAGAGCTTT
    GAGCCGCCAG AGACCCCAGT TGTCAAGGAC AGCACCATCG GTGGCTCACC ACAGCCTCGC
    CCCTCTGTCG GGGCCTTCAA CCCCGGGATG GAGGATATTC TTGACTCTGC AATGGGCACT
    AATTGGGTCC CAGAAGAAGC CTCTGGAGAG GCCAGTGAGA TTCCCGTACC CCAAGGGACA
    GAGCTTTCCC CCTCCAGGCC AGGAGGGGGC AGCATGCAGA CAGAGCCCGC CAGACCCAGC
    AACTTCCTCT CAGCATCTTC TCCACTCCCT GCATCAGCAA AGGGCCAACA GCCGGCAGAT
    GTAACTGGTA CCGCCTTGCC CAGGGTGGGC CCCGTGAGGC CCACTGGCCA GGACTGGAAT
    CACACCCCCC AGAAGACAGA CCATCCATCT GCCCTGCTCA GAGACCCCCC GGAGCCAGGC
    TCTCCCAGGA TCTCATCACT GCGCCCCCAG GGCCTCAGCA ACCCCTCCAC CCTCTCTGCT
    CAGCCACAGC TTTCCAGAAG CCACTCCTCG GGCAGCGTGC TGCCCCTTGG GGAGCTGGAG
    GGCAGGAGGA GCACCAGGGA TCGGAGGAGC CCCGCAGAGC CAGAAGGAGG ACCAGCAAGT
    GAAGGGGCAG CCAGGCCCCT GCCCCGTTTT AACTCCGTTC CTTTGACTGA CACAGGCCAT
    GAGAGGCAGT CCGAGGGATC CTTCAGCCCG CAGCTCCAGG AGTCTGTCTT CCACCTGCTG
    GTGCCCAGTG TCATCCTGGT CTTGCTGGCC GTCGGAGGCC TCTTGTTCTA CAGGTGGAGG
    CGGCGGAGCC ATCAAGAGCC TCAGAGAGCG GATTCTCCCT TGGAGCAACC AGAGGGCAGC
    CCCCTGACTC AGGATGACAG ACAGGTGGAA CTGCCAGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016855858.1
    CDS212..1753
    Translation

    Target ORF information:

    RefSeq Version XM_017000369.1
    Organism Homo sapiens(human)
    Definition Homo sapiens colony stimulating factor 1 (CSF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017000369.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGATTGGGA GTGGACACCT GCAGTCTCTG CAGCGGCTGA TTGACAGTCA GATGGAGACC 
    TCGTGCCAAA TTACATTTGA GTTTGTAGAC CAGGAACAGT TGAAAGATCC AGTGTGCTAC
    CTTAAGAAGG CATTTCTCCT GGTACAAGAC ATAATGGAGG ACACCATGCG CTTCAGAGAT
    AACACCCCCA ATGCCATCGC CATTGTGCAG CTGCAGGAAC TCTCTTTGAG GCTGAAGAGC
    TGCTTCACCA AGGATTATGA AGAGCATGAC AAGGCCTGCG TCCGAACTTT CTATGAGACA
    CCTCTCCAGT TGCTGGAGAA GGTCAAGAAT GTCTTTAATG AAACAAAGAA TCTCCTTGAC
    AAGGACTGGA ATATTTTCAG CAAGAACTGC AACAACAGCT TTGCTGAATG CTCCAGCCAA
    GATGTGGTGA CCAAGCCTGA TTGCAACTGC CTGTACCCCA AAGCCATCCC TAGCAGTGAC
    CCGGCCTCTG TCTCCCCTCA TCAGCCCCTC GCCCCCTCCA TGGCCCCTGT GGCTGGCTTG
    ACCTGGGAGG ACTCTGAGGG AACTGAGGGC AGCTCCCTCT TGCCTGGTGA GCAGCCCCTG
    CACACAGTGG ATCCAGGCAG TGCCAAGCAG CGGCCACCCA GGAGCACCTG CCAGAGCTTT
    GAGCCGCCAG AGACCCCAGT TGTCAAGGAC AGCACCATCG GTGGCTCACC ACAGCCTCGC
    CCCTCTGTCG GGGCCTTCAA CCCCGGGATG GAGGATATTC TTGACTCTGC AATGGGCACT
    AATTGGGTCC CAGAAGAAGC CTCTGGAGAG GCCAGTGAGA TTCCCGTACC CCAAGGGACA
    GAGCTTTCCC CCTCCAGGCC AGGAGGGGGC AGCATGCAGA CAGAGCCCGC CAGACCCAGC
    AACTTCCTCT CAGCATCTTC TCCACTCCCT GCATCAGCAA AGGGCCAACA GCCGGCAGAT
    GTAACTGGTA CCGCCTTGCC CAGGGTGGGC CCCGTGAGGC CCACTGGCCA GGACTGGAAT
    CACACCCCCC AGAAGACAGA CCATCCATCT GCCCTGCTCA GAGACCCCCC GGAGCCAGGC
    TCTCCCAGGA TCTCATCACT GCGCCCCCAG GGCCTCAGCA ACCCCTCCAC CCTCTCTGCT
    CAGCCACAGC TTTCCAGAAG CCACTCCTCG GGCAGCGTGC TGCCCCTTGG GGAGCTGGAG
    GGCAGGAGGA GCACCAGGGA TCGGAGGAGC CCCGCAGAGC CAGAAGGAGG ACCAGCAAGT
    GAAGGGGCAG CCAGGCCCCT GCCCCGTTTT AACTCCGTTC CTTTGACTGA CACAGGCCAT
    GAGAGGCAGT CCGAGGGATC CTTCAGCCCG CAGCTCCAGG AGTCTGTCTT CCACCTGCTG
    GTGCCCAGTG TCATCCTGGT CTTGCTGGCC GTCGGAGGCC TCTTGTTCTA CAGGTGGAGG
    CGGCGGAGCC ATCAAGAGCC TCAGAGAGCG GATTCTCCCT TGGAGCAACC AGAGGGCAGC
    CCCCTGACTC AGGATGACAG ACAGGTGGAA CTGCCAGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.