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CST3 cDNA ORF clone, Homo sapiens (human)

Gene Symbol CST3
Entrez Gene ID 1471
Full Name cystatin C
Synonyms ARMD11
General protein information
Preferred Names
cystatin-C
Names
cystatin-C
cystatin 3
cystatin-3
gamma-trace
post-gamma-globulin
bA218C14.4 (cystatin C)
neuroendocrine basic polypeptide
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

20

20p11.21

Summary The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associated with amyloid angiopathy. Expression of this protein in vascular wall smooth muscle cells is severely reduced in both atherosclerotic and aneurysmal aortic lesions, establishing its role in vascular disease. In addition, this protein has been shown to have an antimicrobial function, inhibiting the replication of herpes simplex virus. Alternative splicing results in multiple transcript variants encoding a single protein. [provided by RefSeq, Nov 2014]. lac of sum
Disorder MIM:

604312

Disorder Html: Cerebral amyloid angiopathy, 105150 (3); Macular degeneration,

mRNA and Protein(s)

mRNA Protein Name
NM_000099 NP_000090 cystatin-C precursor
NM_001288614 NP_001275543 cystatin-C precursor



Homo sapiens (human) CST3 NP_000090.1
Pan troglodytes (chimpanzee) CST3 XP_001146702.2
Macaca mulatta (Rhesus monkey) CST3 NP_001028096.1
Canis lupus familiaris (dog) LOC607874 XP_003639869.1
Bos taurus (cattle) CST3 NP_776454.1
Mus musculus (house mouse) Cst3 NP_034106.2
Rattus norvegicus (Norway rat) Cst3 NP_036969.1
Gallus gallus (chicken) CST3 NP_990831.2
Xenopus (Silurana) tropicalis (western clawed frog) LOC100485774 XP_002934915.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CST3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CST3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu22520
NM_000099 Homo sapiens cystatin C (CST3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu22520
NM_001288614 Homo sapiens cystatin C (CST3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22520
Clone ID Related Accession (Same CDS sequence) NM_000099 , NM_001288614
Accession Version NM_000099.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 441bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cystatin-C precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY042866.1 and BC013083.1. This sequence is a reference standard in the RefSeqGene project. On Dec 24, 2013 this sequence version replaced gi:19882253. Transcript Variant: This variant (1) differs in the 3' UTR compared to variant 2. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## Protein has antimicrobial activity :: PMID: 2153254 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BC110305.1, BI760957.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
ATGGCCGGGC CCCTGCGCGC CCCGCTGCTC CTGCTGGCCA TCCTGGCCGT GGCCCTGGCC 
GTGAGCCCCG CGGCCGGCTC CAGTCCCGGC AAGCCGCCGC GCCTGGTGGG AGGCCCCATG
GACGCCAGCG TGGAGGAGGA GGGTGTGCGG CGTGCACTGG ACTTTGCCGT CGGCGAGTAC
AACAAAGCCA GCAACGACAT GTACCACAGC CGCGCGCTGC AGGTGGTGCG CGCCCGCAAG
CAGATCGTAG CTGGGGTGAA CTACTTCTTG GACGTGGAGC TGGGCCGAAC CACGTGTACC
AAGACCCAGC CCAACTTGGA CAACTGCCCC TTCCATGACC AGCCACATCT GAAAAGGAAA
GCATTCTGCT CTTTCCAGAT CTACGCTGTG CCTTGGCAGG GCACAATGAC CTTGTCGAAA
TCCACCTGTC AGGACGCCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000090.1
CDS187..627
Misc Feature(1)178..180(+)
Misc Feature(2)295..615(+)
Misc Feature(3)295..441(+)
Misc Feature(4)295..297(+)
Misc Feature(5)349..351(+)
Misc Feature(6)454..456(+)
Misc Feature(7)568..570(+)
Misc Feature(8)598..600(+)
Exon (1)1..429
Gene:CST3
Gene Synonym:
Exon (2)430..543
Gene:CST3
Gene Synonym:
Exon (3)544..886
Gene:CST3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000099
Organism Homo sapiens (human)
Definition Homo sapiens cystatin C (CST3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000099

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGCCGGGC CCCTGCGCGC CCCGCTGCTC CTGCTGGCCA TCCTGGCCGT GGCCCTGGCC 
GTGAGCCCCG CGGCCGGCTC CAGTCCCGGC AAGCCGCCGC GCCTGGTGGG AGGCCCCATG
GACGCCAGCG TGGAGGAGGA GGGTGTGCGG CGTGCACTGG ACTTTGCCGT CGGCGAGTAC
AACAAAGCCA GCAACGACAT GTACCACAGC CGCGCGCTGC AGGTGGTGCG CGCCCGCAAG
CAGATCGTAG CTGGGGTGAA CTACTTCTTG GACGTGGAGC TGGGCCGAAC CACGTGTACC
AAGACCCAGC CCAACTTGGA CAACTGCCCC TTCCATGACC AGCCACATCT GAAAAGGAAA
GCATTCTGCT CTTTCCAGAT CTACGCTGTG CCTTGGCAGG GCACAATGAC CTTGTCGAAA
TCCACCTGTC AGGACGCCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22520
Clone ID Related Accession (Same CDS sequence) NM_000099 , NM_001288614
Accession Version NM_001288614.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 441bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cystatin-C precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY042866.1, BX647523.1 and BF509512.1. On Dec 24, 2013 this sequence version replaced gi:530425631. Transcript Variant: This variant (2) represents the longer variant. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## Protein has antimicrobial activity :: PMID: 2153254 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BX647523.1, BG468788.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
ATGGCCGGGC CCCTGCGCGC CCCGCTGCTC CTGCTGGCCA TCCTGGCCGT GGCCCTGGCC 
GTGAGCCCCG CGGCCGGCTC CAGTCCCGGC AAGCCGCCGC GCCTGGTGGG AGGCCCCATG
GACGCCAGCG TGGAGGAGGA GGGTGTGCGG CGTGCACTGG ACTTTGCCGT CGGCGAGTAC
AACAAAGCCA GCAACGACAT GTACCACAGC CGCGCGCTGC AGGTGGTGCG CGCCCGCAAG
CAGATCGTAG CTGGGGTGAA CTACTTCTTG GACGTGGAGC TGGGCCGAAC CACGTGTACC
AAGACCCAGC CCAACTTGGA CAACTGCCCC TTCCATGACC AGCCACATCT GAAAAGGAAA
GCATTCTGCT CTTTCCAGAT CTACGCTGTG CCTTGGCAGG GCACAATGAC CTTGTCGAAA
TCCACCTGTC AGGACGCCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001275543.1
CDS187..627
Misc Feature(1)178..180(+)
Misc Feature(2)295..615(+)
Misc Feature(3)295..441(+)
Exon (1)1..429
Gene:CST3
Gene Synonym:
Exon (2)430..543
Gene:CST3
Gene Synonym:
Exon (3)544..629
Gene:CST3
Gene Synonym:
Exon (4)630..2198
Gene:CST3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001288614
Organism Homo sapiens (human)
Definition Homo sapiens cystatin C (CST3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001288614

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGCCGGGC CCCTGCGCGC CCCGCTGCTC CTGCTGGCCA TCCTGGCCGT GGCCCTGGCC 
GTGAGCCCCG CGGCCGGCTC CAGTCCCGGC AAGCCGCCGC GCCTGGTGGG AGGCCCCATG
GACGCCAGCG TGGAGGAGGA GGGTGTGCGG CGTGCACTGG ACTTTGCCGT CGGCGAGTAC
AACAAAGCCA GCAACGACAT GTACCACAGC CGCGCGCTGC AGGTGGTGCG CGCCCGCAAG
CAGATCGTAG CTGGGGTGAA CTACTTCTTG GACGTGGAGC TGGGCCGAAC CACGTGTACC
AAGACCCAGC CCAACTTGGA CAACTGCCCC TTCCATGACC AGCCACATCT GAAAAGGAAA
GCATTCTGCT CTTTCCAGAT CTACGCTGTG CCTTGGCAGG GCACAATGAC CTTGTCGAAA
TCCACCTGTC AGGACGCCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Polymorphism of the cystatin C gene in patients with acute coronary syndromes: Results from the PLATelet inhibition and patient Outcomes study
Am. Heart J. 168 (1), 96-102 (2014)
Akerblom A, Eriksson N, Wallentin L, Siegbahn A, Barratt BJ, Becker RC, Budaj A, Himmelmann A, Husted S, Storey RF, Johansson A and James SK.


book

Regulation of TGF-beta1-driven differentiation of human lung fibroblasts: emerging roles of cathepsin B and cystatin C
J. Biol. Chem. 289 (23), 16239-16251 (2014)
Kasabova M, Joulin-Giet A, Lecaille F, Gilmore BF, Marchand-Adam S, Saidi A and Lalmanach G.


book

Plasma neutrophil gelatinase-associated lipocalin (NGAL) and plasma cystatin C (CysC) as biomarker of acute kidney injury after cardiac surgery
Saudi J Kidney Dis Transpl 25 (3), 582-588 (2014)
Ghonemy TA and Amro GM.


book

Fertility defects in mice expressing the L68Q variant of human cystatin C: a role for amyloid in male infertility
J. Biol. Chem. 289 (11), 7718-7729 (2014)
Whelly S, Serobian G, Borchardt C, Powell J, Johnson S, Hakansson K, Lindstrom V, Abrahamson M, Grubb A and Cornwall GA.


book

Heart failure mortality according to acute variations in N-terminal pro B-type natriuretic peptide and cystatin C levels
J Cardiovasc Med (Hagerstown) 15 (2), 115-121 (2014)
Carrasco-Sanchez FJ, Perez-Calvo JI, Morales-Rull JL, Galisteo-Almeda L, Paez-Rubio I, Baron-Franco B, Aguayo-Canela M and Pujol-De la llave E.


book

Hereditary cystatin C amyloid angiopathy: identification of the disease-causing mutation and specific diagnosis by polymerase chain reaction based analysis
Hum. Genet. 89 (4), 377-380 (1992)
Abrahamson M, Jonsdottir S, Olafsson I, Jensson O and Grubb A.


book

Interaction of recombinant human cystatin C with the cysteine proteinases papain and actinidin
Biochem. J. 281 (PT 1), 49-55 (1992)
Lindahl P, Abrahamson M and Bjork I.


book

Inactivation of human cystatin C and kininogen by human cathepsin D
FEBS Lett. 280 (2), 211-215 (1991)
Lenarcic B, Krasovec M, Ritonja A, Olafsson I and Turk V.


book

Human cystatin C. role of the N-terminal segment in the inhibition of human cysteine proteinases and in its inactivation by leucocyte elastase
Biochem. J. 273 (PT 3), 621-626 (1991)
Abrahamson M, Mason RW, Hansson H, Buttle DJ, Grubb A and Ohlsson K.


book

Cystatin C, a human proteinase inhibitor, blocks replication of herpes simplex virus
J. Virol. 64 (2), 941-943 (1990)
Bjorck L, Grubb A and Kjellen L.


 
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