RASGEF1B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Gene Symbol	RASGEF1B
Entrez Gene ID	153020
Full Name	RasGEF domain family member 1B
Synonyms	GPIG4
General protein information	Preferred Names RasGEF domain family member 1B Names ras-GEF domain-containing family member 1B GPI gamma-4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q21.21
Disorder MIM:	614532

mRNA and Protein(s)

mRNA	Protein	Name
NM_152545.2	NP_689758.1	ras-GEF domain-containing family member 1B isoform 1
XM_017007813.1	XP_016863302.1	ras-GEF domain-containing family member 1B isoform X2
NM_001300736.1	NP_001287665.1	ras-GEF domain-containing family member 1B isoform 3
NM_001300736.2	NP_001287665.1	ras-GEF domain-containing family member 1B isoform 3
XM_017007812.1	XP_016863301.1	ras-GEF domain-containing family member 1B isoform X1
NM_152545.3	NP_689758.1	ras-GEF domain-containing family member 1B isoform 1
NM_001300735.1	NP_001287664.1	ras-GEF domain-containing family member 1B isoform 2
NM_001300735.2	NP_001287664.1	ras-GEF domain-containing family member 1B isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	RASGEF1B	NP_689758.1
Pan troglodytes (chimpanzee)	RASGEF1B	XP_526570.3
Caenorhabditis elegans (roundworm)	R05G6.10	NP_501217.2
Canis lupus familiaris (dog)	RASGEF1B	XP_005639172.1
Bos taurus (cattle)	RASGEF1B	NP_001077118.1
Mus musculus (house mouse)	Rasgef1b	NP_665838.1
Gallus gallus (chicken)	RASGEF1B	XP_003641217.2
Danio rerio (zebrafish)	rasgef1ba	NP_956123.1
Rattus norvegicus (Norway rat)	Rasgef1b	XP_006250753.1
Drosophila melanogaster (fruit fly)	CG7369	NP_649415.2
Xenopus tropicalis (tropical clawed frog)	rasgef1b	NP_001016913.1

Gene Ontology
Bibliography

Related articles in PubMed

Inducible RasGEF1B circular RNA is a positive regulator of ICAM-1 in the TLR4/LPS pathway.
Ng WL, Marinov GK, Liau ES, Lam YL, Lim YY, Ea CK
RNA biology13(9)861-71(2016 09)

Meta-analysis of genome-wide association studies of adult height in East Asians identifies 17 novel loci.
He M, Xu M, Zhang B, Liang J, Chen P, Lee JY, Johnson TA, Li H, Yang X, Dai J, Liang L, Gui L, Qi Q, Huang J, Li Y, Adair LS, Aung T, Cai Q, Cheng CY, Cho MC, Cho YS, Chu M, Cui B, Gao YT, Go MJ, Gu D, Gu W, Guo H, Hao Y, Hong J, Hu Z, Hu Y, Huang J, Hwang JY, Ikram MK, Jin G, Kang DH, Khor CC, Kim BJ, Kim HT, Kubo M, Lee J, Lee J, Lee NR, Li R, Li J, Liu J, Longe J, Lu W, Lu X, Miao X, Okada Y, Ong RT, Qiu G, Seielstad M, Sim X, Song H, Takeuchi F, Tanaka T, Taylor PR, Wang L, Wang W, Wang Y, Wu C, Wu Y, Xiang YB, Yamamoto K, Yang H, Liao M, Yokota M, Young T, Zhang X, Kato N, Wang QK, Zheng W, Hu FB, Lin D, Shen H, Teo YY, Mo Z, Wong TY, Lin X, Mohlke KL, Ning G, Tsunoda T, Han BG, Shu XO, Tai ES, Wu T, Qi L
Human molecular genetics24(6)1791-800(2015 Mar)

Common genetic variation and antidepressant efficacy in major depressive disorder: a meta-analysis of three genome-wide pharmacogenetic studies.
, ,
The American journal of psychiatry170(2)207-17(2013 Feb)

8.6Mb interstitial deletion of chromosome 4q13.3q21.23 in a boy with cognitive impairment, short stature, hearing loss, skeletal abnormalities and facial dysmorphism.
Lipska BS, Brzeskwiniewicz M, Wierzba J, Morzuchi L, Piotrowski A, Limon J
Genetic counseling (Geneva, Switzerland)22(4)353-63(2011)

RasGEF1A and RasGEF1B are guanine nucleotide exchange factors that discriminate between Rap GTP-binding proteins and mediate Rap2-specific nucleotide exchange.
Yaman E, Gasper R, Koerner C, Wittinghofer A, Tazebay UH
The FEBS journal276(16)4607-16(2009 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We show that knockdown of the expression of mcircRasGEF1B reduces LPS-induced ICAM-1 expression. Additionally, we demonstrate that mcircRasGEF1B regulates the stability of mature ICAM-1 mRNAs.
Title: Inducible RasGEF1B circular RNA is a positive regulator of ICAM-1 in the TLR4/LPS pathway.

As an addition to PRKG2 and RASGEFIB genes, we propose to include BMP3 gene as the principal determinant of the observed common phenotype.
Title: 8.6Mb interstitial deletion of chromosome 4q13.3q21.23 in a boy with cognitive impairment, short stature, hearing loss, skeletal abnormalities and facial dysmorphism.

Mutation of the Ser39 in Rap1 changed the specificity and allowed the nucleotide exchange of Rap1(S39F) to be stimulated by RasGEF1B
Title: RasGEF1A and RasGEF1B are guanine nucleotide exchange factors that discriminate between Rap GTP-binding proteins and mediate Rap2-specific nucleotide exchange.

The following RASGEF1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASGEF1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06834	NM_152545.3 Latest version!	Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu38732	NM_001300735.2 Latest version!	Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu38734	NM_001300736.2 Latest version!	Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu06834	XM_017007813.1 Latest version!	Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu38733	XM_017007812.1 Latest version!	Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu38734	NM_001300736.1	Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu06834	NM_152545.2	Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu38732	NM_001300735.1	Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RASGEF1B ( NM_152545.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_689758.1 Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 1, mRNA.

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CloneID	OHu06834
Clone ID Related Accession (Same CDS sequence)	NM_152545.2 , XM_017007813.1 , NM_152545.3
Accession Version	NM_152545.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Homo sapiens(human)
Product	ras-GEF domain-containing family member 1B isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK056257.1 and AC006287.1. On Jun 2, 2019 this sequence version replaced NM_152545.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK056257.1, SRR1803614.95265.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT 
GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAGA 
ACATACATAT TTACCTTCCT ACTCAGTTCT CGGTTATTTA TGCATCCGTA TGAGCTAATG 
GCCAAAGTTT GCCACTTATG TGTTGAGCAC CAGAGACTAA GTGATCCTGA TAGTGATAAG 
AACCAGATGA GAAAAATTGC ACCCAAAATC CTTCAACTCC TCACGGAATG GACGGAAACA 
TTTCCCTATG ATTTTCGGGA TGAAAGAATG ATGAGAAACT TAAAAGATCT GGCTCACCGA 
ATAGCCAGTG GCGAAGAGCA GACATACAGA AAGAATGTCC AGCAAATGAT GCAGTGTCTG 
ATCCGCAAGC TTGCTGCGCT CAGCCAGTAC GAAGAAGTCC TGGCAAAAAT CAGCTCCACA 
TCCACAGATC GGCTCACAGT TCTCAAGACC AAGCCACAGT CTATACAAAG GGATATCATT 
ACTGTCTGCA ACGACCCTTA CACGTTGGCC CAGCAGCTGA CTCATATAGA GCTGGAGAGG 
CTCAATTATA TTGGGCCAGA AGAATTTGTT CAGGCGTTCG TGCAGAAGGA CCCTTTGGAT 
AATGACAAGA GTTGCTACAG TGAACGGAAG AAAACACGAA ACTTAGAAGC TTACGTGGAA 
TGGTTTAATC GCCTCAGCTA CTTGGTTGCT ACAGAAATCT GTATGCCTGT TAAGAAAAAA 
CACCGAGCAA GAATGATTGA GTATTTCATT GACGTAGCTC GGGAGTGTTT TAACATTGGC 
AACTTCAACT CCTTGATGGC GATAATCTCT GGTATGAATA TGAGCCCAGT CTCTCGACTA 
AAAAAAACTT GGGCCAAAGT GAAGACTGCA AAATTTGACA TTCTTGAGCA TCAGATGGAC 
CCTTCAAGCA ATTTCTATAA TTATCGAACA GCTCTTCGTG GGGCAGCACA AAGGTCTTTA 
ACTGCTCATA GTAGTAGAGA AAAGATTGTG ATACCATTCT TCAGTCTCTT AATCAAAGAT 
ATTTATTTCC TCAATGAGGG TTGTGCCAAC CGCCTTCCCA ATGGCCATGT CAATTTTGAG 
AAATTTTGGG AACTGGCCAA ACAAGTGAGT GAATTTATGA CATGGAAACA AGTGGAGTGT 
CCATTTGAGA GGGACCGGAA GATCTTGCAG TATCTGCTCA CAGTACCAGT CTTCAGTGAA 
GATGCTCTCT ACTTGGCTTC TTATGAGAGT GAAGGACCTG AAAATCATAT AGAGAAAGAC 
AGATGGAAGT CTTTAAGGTC GAGCCTCTTA GGCAGAGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689758.1
CDS	145..1566
Translation	MPQTPPFSAMFDSSGYNRNLYQSAEDSCGGLYYHDNNLLSGSLEALIQHLVPNVDYYPDRTYIFTFLLSSRLFMHPYELMAKVCHLCVEHQRLSDPDSDKNQMRKIAPKILQLLTEWTETFPYDFRDERMMRNLKDLAHRIASGEEQTYRKNVQQMMQCLIRKLAALSQYEEVLAKISSTSTDRLTVLKTKPQSIQRDIITVCNDPYTLAQQLTHIELERLNYIGPEEFVQAFVQKDPLDNDKSCYSERKKTRNLEAYVEWFNRLSYLVATEICMPVKKKHRARMIEYFIDVARECFNIGNFNSLMAIISGMNMSPVSRLKKTWAKVKTAKFDILEHQMDPSSNFYNYRTALRGAAQRSLTAHSSREKIVIPFFSLLIKDIYFLNEGCANRLPNGHVNFEKFWELAKQVSEFMTWKQVECPFERDRKILQYLLTVPVFSEDALYLASYESEGPENHIEKDRWKSLRSSLLGRV

Target ORF information:

RefSeq Version	NM_152545.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152545.3

ORF Insert Sequence:

ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT 
GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAGA 
ACATACATAT TTACCTTCCT ACTCAGTTCT CGGTTATTTA TGCATCCGTA TGAGCTAATG 
GCCAAAGTTT GCCACTTATG TGTTGAGCAC CAGAGACTAA GTGATCCTGA TAGTGATAAG 
AACCAGATGA GAAAAATTGC ACCCAAAATC CTTCAACTCC TCACGGAATG GACGGAAACA 
TTTCCCTATG ATTTTCGGGA TGAAAGAATG ATGAGAAACT TAAAAGATCT GGCTCACCGA 
ATAGCCAGTG GCGAAGAGCA GACATACAGA AAGAATGTCC AGCAAATGAT GCAGTGTCTG 
ATCCGCAAGC TTGCTGCGCT CAGCCAGTAC GAAGAAGTCC TGGCAAAAAT CAGCTCCACA 
TCCACAGATC GGCTCACAGT TCTCAAGACC AAGCCACAGT CTATACAAAG GGATATCATT 
ACTGTCTGCA ACGACCCTTA CACGTTGGCC CAGCAGCTGA CTCATATAGA GCTGGAGAGG 
CTCAATTATA TTGGGCCAGA AGAATTTGTT CAGGCGTTCG TGCAGAAGGA CCCTTTGGAT 
AATGACAAGA GTTGCTACAG TGAACGGAAG AAAACACGAA ACTTAGAAGC TTACGTGGAA 
TGGTTTAATC GCCTCAGCTA CTTGGTTGCT ACAGAAATCT GTATGCCTGT TAAGAAAAAA 
CACCGAGCAA GAATGATTGA GTATTTCATT GACGTAGCTC GGGAGTGTTT TAACATTGGC 
AACTTCAACT CCTTGATGGC GATAATCTCT GGTATGAATA TGAGCCCAGT CTCTCGACTA 
AAAAAAACTT GGGCCAAAGT GAAGACTGCA AAATTTGACA TTCTTGAGCA TCAGATGGAC 
CCTTCAAGCA ATTTCTATAA TTATCGAACA GCTCTTCGTG GGGCAGCACA AAGGTCTTTA 
ACTGCTCATA GTAGTAGAGA AAAGATTGTG ATACCATTCT TCAGTCTCTT AATCAAAGAT 
ATTTATTTCC TCAATGAGGG TTGTGCCAAC CGCCTTCCCA ATGGCCATGT CAATTTTGAG 
AAATTTTGGG AACTGGCCAA ACAAGTGAGT GAATTTATGA CATGGAAACA AGTGGAGTGT 
CCATTTGAGA GGGACCGGAA GATCTTGCAG TATCTGCTCA CAGTACCAGT CTTCAGTGAA 
GATGCTCTCT ACTTGGCTTC TTATGAGAGT GAAGGACCTG AAAATCATAT AGAGAAAGAC 
AGATGGAAGT CTTTAAGGTC GAGCCTCTTA GGCAGAGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGEF1B ( NM_001300735.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287664.1 Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu38732
Clone ID Related Accession (Same CDS sequence)	NM_001300735.1 , NM_001300735.2
Accession Version	NM_001300735.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	ras-GEF domain-containing family member 1B isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DR002387.1, BC121003.1 and AC006287.1. On Jul 11, 2014 this sequence version replaced XM_005262776.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.206051.1, SRR1803613.42258.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT 
GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAGA 
ACATACATAT TTACCTTCCT ACTCAGTTCT CGGTTATTTA TGCATCCGTA TGAGCTAATG 
GCCAAAGTTT GCCACTTATG TGTTGAGCAC CAGAGACTAA GTGATCCTGA TAGTGATAAG 
AACCAGATGA GAAAAATTGC ACCCAAAATC CTTCAACTCC TCACGGAATG GACGGAAACA 
TTTCCCTATG ATTTTCGGGA TGAAAGAATG ATGAGAAACT TAAAAGATCT GGCTCACCGA 
ATAGCCAGTG GCGAAGAGAC ATACAGAAAG AATGTCCAGC AAATGATGCA GTGTCTGATC 
CGCAAGCTTG CTGCGCTCAG CCAGTACGAA GAAGTCCTGG CAAAAATCAG CTCCACATCC 
ACAGATCGGC TCACAGTTCT CAAGACCAAG CCACAGTCTA TACAAAGGGA TATCATTACT 
GTCTGCAACG ACCCTTACAC GTTGGCCCAG CAGCTGACTC ATATAGAGCT GGAGAGGCTC 
AATTATATTG GGCCAGAAGA ATTTGTTCAG GCGTTCGTGC AGAAGGACCC TTTGGATAAT 
GACAAGAGTT GCTACAGTGA ACGGAAGAAA ACACGAAACT TAGAAGCTTA CGTGGAATGG 
TTTAATCGCC TCAGCTACTT GGTTGCTACA GAAATCTGTA TGCCTGTTAA GAAAAAACAC 
CGAGCAAGAA TGATTGAGTA TTTCATTGAC GTAGCTCGGG AGTGTTTTAA CATTGGCAAC 
TTCAACTCCT TGATGGCGAT AATCTCTGGT ATGAATATGA GCCCAGTCTC TCGACTAAAA 
AAAACTTGGG CCAAAGTGAA GACTGCAAAA TTTGACATTC TTGAGCATCA GATGGACCCT 
TCAAGCAATT TCTATAATTA TCGAACAGCT CTTCGTGGGG CAGCACAAAG GTCTTTAACT 
GCTCATAGTA GTAGAGAAAA GATTGTGATA CCATTCTTCA GTCTCTTAAT CAAAGATATT 
TATTTCCTCA ATGAGGGTTG TGCCAACCGC CTTCCCAATG GCCATGTCAA TTTTGAGAAA 
TTTTGGGAAC TGGCCAAACA AGTGAGTGAA TTTATGACAT GGAAACAAGT GGAGTGTCCA 
TTTGAGAGGG ACCGGAAGAT CTTGCAGTAT CTGCTCACAG TACCAGTCTT CAGTGAAGAT 
GCTCTCTACT TGGCTTCTTA TGAGAGTGAA GGACCTGAAA ATCATATAGA GAAAGACAGA 
TGGAAGTCTT TAAGGTCGAG CCTCTTAGGC AGAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287664.1
CDS	166..1584
Translation	MPQTPPFSAMFDSSGYNRNLYQSAEDSCGGLYYHDNNLLSGSLEALIQHLVPNVDYYPDRTYIFTFLLSSRLFMHPYELMAKVCHLCVEHQRLSDPDSDKNQMRKIAPKILQLLTEWTETFPYDFRDERMMRNLKDLAHRIASGEETYRKNVQQMMQCLIRKLAALSQYEEVLAKISSTSTDRLTVLKTKPQSIQRDIITVCNDPYTLAQQLTHIELERLNYIGPEEFVQAFVQKDPLDNDKSCYSERKKTRNLEAYVEWFNRLSYLVATEICMPVKKKHRARMIEYFIDVARECFNIGNFNSLMAIISGMNMSPVSRLKKTWAKVKTAKFDILEHQMDPSSNFYNYRTALRGAAQRSLTAHSSREKIVIPFFSLLIKDIYFLNEGCANRLPNGHVNFEKFWELAKQVSEFMTWKQVECPFERDRKILQYLLTVPVFSEDALYLASYESEGPENHIEKDRWKSLRSSLLGRV

Target ORF information:

RefSeq Version	NM_001300735.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300735.1

ORF Insert Sequence:

ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT 
GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAGA 
ACATACATAT TTACCTTCCT ACTCAGTTCT CGGTTATTTA TGCATCCGTA TGAGCTAATG 
GCCAAAGTTT GCCACTTATG TGTTGAGCAC CAGAGACTAA GTGATCCTGA TAGTGATAAG 
AACCAGATGA GAAAAATTGC ACCCAAAATC CTTCAACTCC TCACGGAATG GACGGAAACA 
TTTCCCTATG ATTTTCGGGA TGAAAGAATG ATGAGAAACT TAAAAGATCT GGCTCACCGA 
ATAGCCAGTG GCGAAGAGAC ATACAGAAAG AATGTCCAGC AAATGATGCA GTGTCTGATC 
CGCAAGCTTG CTGCGCTCAG CCAGTACGAA GAAGTCCTGG CAAAAATCAG CTCCACATCC 
ACAGATCGGC TCACAGTTCT CAAGACCAAG CCACAGTCTA TACAAAGGGA TATCATTACT 
GTCTGCAACG ACCCTTACAC GTTGGCCCAG CAGCTGACTC ATATAGAGCT GGAGAGGCTC 
AATTATATTG GGCCAGAAGA ATTTGTTCAG GCGTTCGTGC AGAAGGACCC TTTGGATAAT 
GACAAGAGTT GCTACAGTGA ACGGAAGAAA ACACGAAACT TAGAAGCTTA CGTGGAATGG 
TTTAATCGCC TCAGCTACTT GGTTGCTACA GAAATCTGTA TGCCTGTTAA GAAAAAACAC 
CGAGCAAGAA TGATTGAGTA TTTCATTGAC GTAGCTCGGG AGTGTTTTAA CATTGGCAAC 
TTCAACTCCT TGATGGCGAT AATCTCTGGT ATGAATATGA GCCCAGTCTC TCGACTAAAA 
AAAACTTGGG CCAAAGTGAA GACTGCAAAA TTTGACATTC TTGAGCATCA GATGGACCCT 
TCAAGCAATT TCTATAATTA TCGAACAGCT CTTCGTGGGG CAGCACAAAG GTCTTTAACT 
GCTCATAGTA GTAGAGAAAA GATTGTGATA CCATTCTTCA GTCTCTTAAT CAAAGATATT 
TATTTCCTCA ATGAGGGTTG TGCCAACCGC CTTCCCAATG GCCATGTCAA TTTTGAGAAA 
TTTTGGGAAC TGGCCAAACA AGTGAGTGAA TTTATGACAT GGAAACAAGT GGAGTGTCCA 
TTTGAGAGGG ACCGGAAGAT CTTGCAGTAT CTGCTCACAG TACCAGTCTT CAGTGAAGAT 
GCTCTCTACT TGGCTTCTTA TGAGAGTGAA GGACCTGAAA ATCATATAGA GAAAGACAGA 
TGGAAGTCTT TAAGGTCGAG CCTCTTAGGC AGAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGEF1B ( NM_001300735.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287664.1 Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu38732
Clone ID Related Accession (Same CDS sequence)	NM_001300735.1 , NM_001300735.2
Accession Version	NM_001300735.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-09
Organism	Homo sapiens(human)
Product	ras-GEF domain-containing family member 1B isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DR002387.1, BC121003.1 and AC006287.1. On Jun 2, 2019 this sequence version replaced NM_001300735.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.42258.1, SRR1660809.206051.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT 
GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAGA 
ACATACATAT TTACCTTCCT ACTCAGTTCT CGGTTATTTA TGCATCCGTA TGAGCTAATG 
GCCAAAGTTT GCCACTTATG TGTTGAGCAC CAGAGACTAA GTGATCCTGA TAGTGATAAG 
AACCAGATGA GAAAAATTGC ACCCAAAATC CTTCAACTCC TCACGGAATG GACGGAAACA 
TTTCCCTATG ATTTTCGGGA TGAAAGAATG ATGAGAAACT TAAAAGATCT GGCTCACCGA 
ATAGCCAGTG GCGAAGAGAC ATACAGAAAG AATGTCCAGC AAATGATGCA GTGTCTGATC 
CGCAAGCTTG CTGCGCTCAG CCAGTACGAA GAAGTCCTGG CAAAAATCAG CTCCACATCC 
ACAGATCGGC TCACAGTTCT CAAGACCAAG CCACAGTCTA TACAAAGGGA TATCATTACT 
GTCTGCAACG ACCCTTACAC GTTGGCCCAG CAGCTGACTC ATATAGAGCT GGAGAGGCTC 
AATTATATTG GGCCAGAAGA ATTTGTTCAG GCGTTCGTGC AGAAGGACCC TTTGGATAAT 
GACAAGAGTT GCTACAGTGA ACGGAAGAAA ACACGAAACT TAGAAGCTTA CGTGGAATGG 
TTTAATCGCC TCAGCTACTT GGTTGCTACA GAAATCTGTA TGCCTGTTAA GAAAAAACAC 
CGAGCAAGAA TGATTGAGTA TTTCATTGAC GTAGCTCGGG AGTGTTTTAA CATTGGCAAC 
TTCAACTCCT TGATGGCGAT AATCTCTGGT ATGAATATGA GCCCAGTCTC TCGACTAAAA 
AAAACTTGGG CCAAAGTGAA GACTGCAAAA TTTGACATTC TTGAGCATCA GATGGACCCT 
TCAAGCAATT TCTATAATTA TCGAACAGCT CTTCGTGGGG CAGCACAAAG GTCTTTAACT 
GCTCATAGTA GTAGAGAAAA GATTGTGATA CCATTCTTCA GTCTCTTAAT CAAAGATATT 
TATTTCCTCA ATGAGGGTTG TGCCAACCGC CTTCCCAATG GCCATGTCAA TTTTGAGAAA 
TTTTGGGAAC TGGCCAAACA AGTGAGTGAA TTTATGACAT GGAAACAAGT GGAGTGTCCA 
TTTGAGAGGG ACCGGAAGAT CTTGCAGTAT CTGCTCACAG TACCAGTCTT CAGTGAAGAT 
GCTCTCTACT TGGCTTCTTA TGAGAGTGAA GGACCTGAAA ATCATATAGA GAAAGACAGA 
TGGAAGTCTT TAAGGTCGAG CCTCTTAGGC AGAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287664.1
CDS	145..1563
Translation	MPQTPPFSAMFDSSGYNRNLYQSAEDSCGGLYYHDNNLLSGSLEALIQHLVPNVDYYPDRTYIFTFLLSSRLFMHPYELMAKVCHLCVEHQRLSDPDSDKNQMRKIAPKILQLLTEWTETFPYDFRDERMMRNLKDLAHRIASGEETYRKNVQQMMQCLIRKLAALSQYEEVLAKISSTSTDRLTVLKTKPQSIQRDIITVCNDPYTLAQQLTHIELERLNYIGPEEFVQAFVQKDPLDNDKSCYSERKKTRNLEAYVEWFNRLSYLVATEICMPVKKKHRARMIEYFIDVARECFNIGNFNSLMAIISGMNMSPVSRLKKTWAKVKTAKFDILEHQMDPSSNFYNYRTALRGAAQRSLTAHSSREKIVIPFFSLLIKDIYFLNEGCANRLPNGHVNFEKFWELAKQVSEFMTWKQVECPFERDRKILQYLLTVPVFSEDALYLASYESEGPENHIEKDRWKSLRSSLLGRV

Target ORF information:

RefSeq Version	NM_001300735.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300735.2

ORF Insert Sequence:

ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT 
GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAGA 
ACATACATAT TTACCTTCCT ACTCAGTTCT CGGTTATTTA TGCATCCGTA TGAGCTAATG 
GCCAAAGTTT GCCACTTATG TGTTGAGCAC CAGAGACTAA GTGATCCTGA TAGTGATAAG 
AACCAGATGA GAAAAATTGC ACCCAAAATC CTTCAACTCC TCACGGAATG GACGGAAACA 
TTTCCCTATG ATTTTCGGGA TGAAAGAATG ATGAGAAACT TAAAAGATCT GGCTCACCGA 
ATAGCCAGTG GCGAAGAGAC ATACAGAAAG AATGTCCAGC AAATGATGCA GTGTCTGATC 
CGCAAGCTTG CTGCGCTCAG CCAGTACGAA GAAGTCCTGG CAAAAATCAG CTCCACATCC 
ACAGATCGGC TCACAGTTCT CAAGACCAAG CCACAGTCTA TACAAAGGGA TATCATTACT 
GTCTGCAACG ACCCTTACAC GTTGGCCCAG CAGCTGACTC ATATAGAGCT GGAGAGGCTC 
AATTATATTG GGCCAGAAGA ATTTGTTCAG GCGTTCGTGC AGAAGGACCC TTTGGATAAT 
GACAAGAGTT GCTACAGTGA ACGGAAGAAA ACACGAAACT TAGAAGCTTA CGTGGAATGG 
TTTAATCGCC TCAGCTACTT GGTTGCTACA GAAATCTGTA TGCCTGTTAA GAAAAAACAC 
CGAGCAAGAA TGATTGAGTA TTTCATTGAC GTAGCTCGGG AGTGTTTTAA CATTGGCAAC 
TTCAACTCCT TGATGGCGAT AATCTCTGGT ATGAATATGA GCCCAGTCTC TCGACTAAAA 
AAAACTTGGG CCAAAGTGAA GACTGCAAAA TTTGACATTC TTGAGCATCA GATGGACCCT 
TCAAGCAATT TCTATAATTA TCGAACAGCT CTTCGTGGGG CAGCACAAAG GTCTTTAACT 
GCTCATAGTA GTAGAGAAAA GATTGTGATA CCATTCTTCA GTCTCTTAAT CAAAGATATT 
TATTTCCTCA ATGAGGGTTG TGCCAACCGC CTTCCCAATG GCCATGTCAA TTTTGAGAAA 
TTTTGGGAAC TGGCCAAACA AGTGAGTGAA TTTATGACAT GGAAACAAGT GGAGTGTCCA 
TTTGAGAGGG ACCGGAAGAT CTTGCAGTAT CTGCTCACAG TACCAGTCTT CAGTGAAGAT 
GCTCTCTACT TGGCTTCTTA TGAGAGTGAA GGACCTGAAA ATCATATAGA GAAAGACAGA 
TGGAAGTCTT TAAGGTCGAG CCTCTTAGGC AGAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGEF1B ( NM_152545.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_689758.1 Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 1, mRNA.

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CloneID	OHu06834
Clone ID Related Accession (Same CDS sequence)	NM_152545.2 , XM_017007813.1 , NM_152545.3
Accession Version	NM_152545.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	ras-GEF domain-containing family member 1B isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DR002387.1, AK056257.1 and AC006287.1. On Jul 11, 2014 this sequence version replaced NM_152545.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.158476.1, SRR1803614.95265.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT 
GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAGA 
ACATACATAT TTACCTTCCT ACTCAGTTCT CGGTTATTTA TGCATCCGTA TGAGCTAATG 
GCCAAAGTTT GCCACTTATG TGTTGAGCAC CAGAGACTAA GTGATCCTGA TAGTGATAAG 
AACCAGATGA GAAAAATTGC ACCCAAAATC CTTCAACTCC TCACGGAATG GACGGAAACA 
TTTCCCTATG ATTTTCGGGA TGAAAGAATG ATGAGAAACT TAAAAGATCT GGCTCACCGA 
ATAGCCAGTG GCGAAGAGCA GACATACAGA AAGAATGTCC AGCAAATGAT GCAGTGTCTG 
ATCCGCAAGC TTGCTGCGCT CAGCCAGTAC GAAGAAGTCC TGGCAAAAAT CAGCTCCACA 
TCCACAGATC GGCTCACAGT TCTCAAGACC AAGCCACAGT CTATACAAAG GGATATCATT 
ACTGTCTGCA ACGACCCTTA CACGTTGGCC CAGCAGCTGA CTCATATAGA GCTGGAGAGG 
CTCAATTATA TTGGGCCAGA AGAATTTGTT CAGGCGTTCG TGCAGAAGGA CCCTTTGGAT 
AATGACAAGA GTTGCTACAG TGAACGGAAG AAAACACGAA ACTTAGAAGC TTACGTGGAA 
TGGTTTAATC GCCTCAGCTA CTTGGTTGCT ACAGAAATCT GTATGCCTGT TAAGAAAAAA 
CACCGAGCAA GAATGATTGA GTATTTCATT GACGTAGCTC GGGAGTGTTT TAACATTGGC 
AACTTCAACT CCTTGATGGC GATAATCTCT GGTATGAATA TGAGCCCAGT CTCTCGACTA 
AAAAAAACTT GGGCCAAAGT GAAGACTGCA AAATTTGACA TTCTTGAGCA TCAGATGGAC 
CCTTCAAGCA ATTTCTATAA TTATCGAACA GCTCTTCGTG GGGCAGCACA AAGGTCTTTA 
ACTGCTCATA GTAGTAGAGA AAAGATTGTG ATACCATTCT TCAGTCTCTT AATCAAAGAT 
ATTTATTTCC TCAATGAGGG TTGTGCCAAC CGCCTTCCCA ATGGCCATGT CAATTTTGAG 
AAATTTTGGG AACTGGCCAA ACAAGTGAGT GAATTTATGA CATGGAAACA AGTGGAGTGT 
CCATTTGAGA GGGACCGGAA GATCTTGCAG TATCTGCTCA CAGTACCAGT CTTCAGTGAA 
GATGCTCTCT ACTTGGCTTC TTATGAGAGT GAAGGACCTG AAAATCATAT AGAGAAAGAC 
AGATGGAAGT CTTTAAGGTC GAGCCTCTTA GGCAGAGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689758.1
CDS	166..1587
Translation	MPQTPPFSAMFDSSGYNRNLYQSAEDSCGGLYYHDNNLLSGSLEALIQHLVPNVDYYPDRTYIFTFLLSSRLFMHPYELMAKVCHLCVEHQRLSDPDSDKNQMRKIAPKILQLLTEWTETFPYDFRDERMMRNLKDLAHRIASGEEQTYRKNVQQMMQCLIRKLAALSQYEEVLAKISSTSTDRLTVLKTKPQSIQRDIITVCNDPYTLAQQLTHIELERLNYIGPEEFVQAFVQKDPLDNDKSCYSERKKTRNLEAYVEWFNRLSYLVATEICMPVKKKHRARMIEYFIDVARECFNIGNFNSLMAIISGMNMSPVSRLKKTWAKVKTAKFDILEHQMDPSSNFYNYRTALRGAAQRSLTAHSSREKIVIPFFSLLIKDIYFLNEGCANRLPNGHVNFEKFWELAKQVSEFMTWKQVECPFERDRKILQYLLTVPVFSEDALYLASYESEGPENHIEKDRWKSLRSSLLGRV

Target ORF information:

RefSeq Version	NM_152545.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152545.2

ORF Insert Sequence:

ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT 
GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAGA 
ACATACATAT TTACCTTCCT ACTCAGTTCT CGGTTATTTA TGCATCCGTA TGAGCTAATG 
GCCAAAGTTT GCCACTTATG TGTTGAGCAC CAGAGACTAA GTGATCCTGA TAGTGATAAG 
AACCAGATGA GAAAAATTGC ACCCAAAATC CTTCAACTCC TCACGGAATG GACGGAAACA 
TTTCCCTATG ATTTTCGGGA TGAAAGAATG ATGAGAAACT TAAAAGATCT GGCTCACCGA 
ATAGCCAGTG GCGAAGAGCA GACATACAGA AAGAATGTCC AGCAAATGAT GCAGTGTCTG 
ATCCGCAAGC TTGCTGCGCT CAGCCAGTAC GAAGAAGTCC TGGCAAAAAT CAGCTCCACA 
TCCACAGATC GGCTCACAGT TCTCAAGACC AAGCCACAGT CTATACAAAG GGATATCATT 
ACTGTCTGCA ACGACCCTTA CACGTTGGCC CAGCAGCTGA CTCATATAGA GCTGGAGAGG 
CTCAATTATA TTGGGCCAGA AGAATTTGTT CAGGCGTTCG TGCAGAAGGA CCCTTTGGAT 
AATGACAAGA GTTGCTACAG TGAACGGAAG AAAACACGAA ACTTAGAAGC TTACGTGGAA 
TGGTTTAATC GCCTCAGCTA CTTGGTTGCT ACAGAAATCT GTATGCCTGT TAAGAAAAAA 
CACCGAGCAA GAATGATTGA GTATTTCATT GACGTAGCTC GGGAGTGTTT TAACATTGGC 
AACTTCAACT CCTTGATGGC GATAATCTCT GGTATGAATA TGAGCCCAGT CTCTCGACTA 
AAAAAAACTT GGGCCAAAGT GAAGACTGCA AAATTTGACA TTCTTGAGCA TCAGATGGAC 
CCTTCAAGCA ATTTCTATAA TTATCGAACA GCTCTTCGTG GGGCAGCACA AAGGTCTTTA 
ACTGCTCATA GTAGTAGAGA AAAGATTGTG ATACCATTCT TCAGTCTCTT AATCAAAGAT 
ATTTATTTCC TCAATGAGGG TTGTGCCAAC CGCCTTCCCA ATGGCCATGT CAATTTTGAG 
AAATTTTGGG AACTGGCCAA ACAAGTGAGT GAATTTATGA CATGGAAACA AGTGGAGTGT 
CCATTTGAGA GGGACCGGAA GATCTTGCAG TATCTGCTCA CAGTACCAGT CTTCAGTGAA 
GATGCTCTCT ACTTGGCTTC TTATGAGAGT GAAGGACCTG AAAATCATAT AGAGAAAGAC 
AGATGGAAGT CTTTAAGGTC GAGCCTCTTA GGCAGAGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGEF1B ( NM_001300736.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287665.1 Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 3, mRNA.

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CloneID	OHu38734
Clone ID Related Accession (Same CDS sequence)	NM_001300736.1 , NM_001300736.2
Accession Version	NM_001300736.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	ras-GEF domain-containing family member 1B isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DR002387.1, BC121004.1 and AC006287.1. On Jul 11, 2014 this sequence version replaced XM_005262777.1. Transcript Variant: This variant (3) lacks an in-frame exon and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK303625.1, BC121004.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT 
GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAAC 
CAGATGAGAA AAATTGCACC CAAAATCCTT CAACTCCTCA CGGAATGGAC GGAAACATTT 
CCCTATGATT TTCGGGATGA AAGAATGATG AGAAACTTAA AAGATCTGGC TCACCGAATA 
GCCAGTGGCG AAGAGACATA CAGAAAGAAT GTCCAGCAAA TGATGCAGTG TCTGATCCGC 
AAGCTTGCTG CGCTCAGCCA GTACGAAGAA GTCCTGGCAA AAATCAGCTC CACATCCACA 
GATCGGCTCA CAGTTCTCAA GACCAAGCCA CAGTCTATAC AAAGGGATAT CATTACTGTC 
TGCAACGACC CTTACACGTT GGCCCAGCAG CTGACTCATA TAGAGCTGGA GAGGCTCAAT 
TATATTGGGC CAGAAGAATT TGTTCAGGCG TTCGTGCAGA AGGACCCTTT GGATAATGAC 
AAGAGTTGCT ACAGTGAACG GAAGAAAACA CGAAACTTAG AAGCTTACGT GGAATGGTTT 
AATCGCCTCA GCTACTTGGT TGCTACAGAA ATCTGTATGC CTGTTAAGAA AAAACACCGA 
GCAAGAATGA TTGAGTATTT CATTGACGTA GCTCGGGAGT GTTTTAACAT TGGCAACTTC 
AACTCCTTGA TGGCGATAAT CTCTGGTATG AATATGAGCC CAGTCTCTCG ACTAAAAAAA 
ACTTGGGCCA AAGTGAAGAC TGCAAAATTT GACATTCTTG AGCATCAGAT GGACCCTTCA 
AGCAATTTCT ATAATTATCG AACAGCTCTT CGTGGGGCAG CACAAAGGTC TTTAACTGCT 
CATAGTAGTA GAGAAAAGAT TGTGATACCA TTCTTCAGTC TCTTAATCAA AGATATTTAT 
TTCCTCAATG AGGGTTGTGC CAACCGCCTT CCCAATGGCC ATGTCAATTT TGAGAAATTT 
TGGGAACTGG CCAAACAAGT GAGTGAATTT ATGACATGGA AACAAGTGGA GTGTCCATTT 
GAGAGGGACC GGAAGATCTT GCAGTATCTG CTCACAGTAC CAGTCTTCAG TGAAGATGCT 
CTCTACTTGG CTTCTTATGA GAGTGAAGGA CCTGAAAATC ATATAGAGAA AGACAGATGG 
AAGTCTTTAA GGTCGAGCCT CTTAGGCAGA GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287665.1
CDS	166..1461
Translation	MPQTPPFSAMFDSSGYNRNLYQSAEDSCGGLYYHDNNLLSGSLEALIQHLVPNVDYYPDNQMRKIAPKILQLLTEWTETFPYDFRDERMMRNLKDLAHRIASGEETYRKNVQQMMQCLIRKLAALSQYEEVLAKISSTSTDRLTVLKTKPQSIQRDIITVCNDPYTLAQQLTHIELERLNYIGPEEFVQAFVQKDPLDNDKSCYSERKKTRNLEAYVEWFNRLSYLVATEICMPVKKKHRARMIEYFIDVARECFNIGNFNSLMAIISGMNMSPVSRLKKTWAKVKTAKFDILEHQMDPSSNFYNYRTALRGAAQRSLTAHSSREKIVIPFFSLLIKDIYFLNEGCANRLPNGHVNFEKFWELAKQVSEFMTWKQVECPFERDRKILQYLLTVPVFSEDALYLASYESEGPENHIEKDRWKSLRSSLLGRV

Target ORF information:

RefSeq Version	NM_001300736.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300736.1

ORF Insert Sequence:

ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT 
GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAAC 
CAGATGAGAA AAATTGCACC CAAAATCCTT CAACTCCTCA CGGAATGGAC GGAAACATTT 
CCCTATGATT TTCGGGATGA AAGAATGATG AGAAACTTAA AAGATCTGGC TCACCGAATA 
GCCAGTGGCG AAGAGACATA CAGAAAGAAT GTCCAGCAAA TGATGCAGTG TCTGATCCGC 
AAGCTTGCTG CGCTCAGCCA GTACGAAGAA GTCCTGGCAA AAATCAGCTC CACATCCACA 
GATCGGCTCA CAGTTCTCAA GACCAAGCCA CAGTCTATAC AAAGGGATAT CATTACTGTC 
TGCAACGACC CTTACACGTT GGCCCAGCAG CTGACTCATA TAGAGCTGGA GAGGCTCAAT 
TATATTGGGC CAGAAGAATT TGTTCAGGCG TTCGTGCAGA AGGACCCTTT GGATAATGAC 
AAGAGTTGCT ACAGTGAACG GAAGAAAACA CGAAACTTAG AAGCTTACGT GGAATGGTTT 
AATCGCCTCA GCTACTTGGT TGCTACAGAA ATCTGTATGC CTGTTAAGAA AAAACACCGA 
GCAAGAATGA TTGAGTATTT CATTGACGTA GCTCGGGAGT GTTTTAACAT TGGCAACTTC 
AACTCCTTGA TGGCGATAAT CTCTGGTATG AATATGAGCC CAGTCTCTCG ACTAAAAAAA 
ACTTGGGCCA AAGTGAAGAC TGCAAAATTT GACATTCTTG AGCATCAGAT GGACCCTTCA 
AGCAATTTCT ATAATTATCG AACAGCTCTT CGTGGGGCAG CACAAAGGTC TTTAACTGCT 
CATAGTAGTA GAGAAAAGAT TGTGATACCA TTCTTCAGTC TCTTAATCAA AGATATTTAT 
TTCCTCAATG AGGGTTGTGC CAACCGCCTT CCCAATGGCC ATGTCAATTT TGAGAAATTT 
TGGGAACTGG CCAAACAAGT GAGTGAATTT ATGACATGGA AACAAGTGGA GTGTCCATTT 
GAGAGGGACC GGAAGATCTT GCAGTATCTG CTCACAGTAC CAGTCTTCAG TGAAGATGCT 
CTCTACTTGG CTTCTTATGA GAGTGAAGGA CCTGAAAATC ATATAGAGAA AGACAGATGG 
AAGTCTTTAA GGTCGAGCCT CTTAGGCAGA GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGEF1B ( NM_001300736.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287665.1 Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu38734
Clone ID Related Accession (Same CDS sequence)	NM_001300736.1 , NM_001300736.2
Accession Version	NM_001300736.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Homo sapiens(human)
Product	ras-GEF domain-containing family member 1B isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DR002387.1, BC121004.1 and AC006287.1. On Jun 2, 2019 this sequence version replaced NM_001300736.1. Transcript Variant: This variant (3) lacks an in-frame exon and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC121004.1, AK303625.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT 
GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAAC 
CAGATGAGAA AAATTGCACC CAAAATCCTT CAACTCCTCA CGGAATGGAC GGAAACATTT 
CCCTATGATT TTCGGGATGA AAGAATGATG AGAAACTTAA AAGATCTGGC TCACCGAATA 
GCCAGTGGCG AAGAGACATA CAGAAAGAAT GTCCAGCAAA TGATGCAGTG TCTGATCCGC 
AAGCTTGCTG CGCTCAGCCA GTACGAAGAA GTCCTGGCAA AAATCAGCTC CACATCCACA 
GATCGGCTCA CAGTTCTCAA GACCAAGCCA CAGTCTATAC AAAGGGATAT CATTACTGTC 
TGCAACGACC CTTACACGTT GGCCCAGCAG CTGACTCATA TAGAGCTGGA GAGGCTCAAT 
TATATTGGGC CAGAAGAATT TGTTCAGGCG TTCGTGCAGA AGGACCCTTT GGATAATGAC 
AAGAGTTGCT ACAGTGAACG GAAGAAAACA CGAAACTTAG AAGCTTACGT GGAATGGTTT 
AATCGCCTCA GCTACTTGGT TGCTACAGAA ATCTGTATGC CTGTTAAGAA AAAACACCGA 
GCAAGAATGA TTGAGTATTT CATTGACGTA GCTCGGGAGT GTTTTAACAT TGGCAACTTC 
AACTCCTTGA TGGCGATAAT CTCTGGTATG AATATGAGCC CAGTCTCTCG ACTAAAAAAA 
ACTTGGGCCA AAGTGAAGAC TGCAAAATTT GACATTCTTG AGCATCAGAT GGACCCTTCA 
AGCAATTTCT ATAATTATCG AACAGCTCTT CGTGGGGCAG CACAAAGGTC TTTAACTGCT 
CATAGTAGTA GAGAAAAGAT TGTGATACCA TTCTTCAGTC TCTTAATCAA AGATATTTAT 
TTCCTCAATG AGGGTTGTGC CAACCGCCTT CCCAATGGCC ATGTCAATTT TGAGAAATTT 
TGGGAACTGG CCAAACAAGT GAGTGAATTT ATGACATGGA AACAAGTGGA GTGTCCATTT 
GAGAGGGACC GGAAGATCTT GCAGTATCTG CTCACAGTAC CAGTCTTCAG TGAAGATGCT 
CTCTACTTGG CTTCTTATGA GAGTGAAGGA CCTGAAAATC ATATAGAGAA AGACAGATGG 
AAGTCTTTAA GGTCGAGCCT CTTAGGCAGA GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287665.1
CDS	145..1440
Translation	MPQTPPFSAMFDSSGYNRNLYQSAEDSCGGLYYHDNNLLSGSLEALIQHLVPNVDYYPDNQMRKIAPKILQLLTEWTETFPYDFRDERMMRNLKDLAHRIASGEETYRKNVQQMMQCLIRKLAALSQYEEVLAKISSTSTDRLTVLKTKPQSIQRDIITVCNDPYTLAQQLTHIELERLNYIGPEEFVQAFVQKDPLDNDKSCYSERKKTRNLEAYVEWFNRLSYLVATEICMPVKKKHRARMIEYFIDVARECFNIGNFNSLMAIISGMNMSPVSRLKKTWAKVKTAKFDILEHQMDPSSNFYNYRTALRGAAQRSLTAHSSREKIVIPFFSLLIKDIYFLNEGCANRLPNGHVNFEKFWELAKQVSEFMTWKQVECPFERDRKILQYLLTVPVFSEDALYLASYESEGPENHIEKDRWKSLRSSLLGRV

Target ORF information:

RefSeq Version	NM_001300736.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300736.2

ORF Insert Sequence:

ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT 
GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAAC 
CAGATGAGAA AAATTGCACC CAAAATCCTT CAACTCCTCA CGGAATGGAC GGAAACATTT 
CCCTATGATT TTCGGGATGA AAGAATGATG AGAAACTTAA AAGATCTGGC TCACCGAATA 
GCCAGTGGCG AAGAGACATA CAGAAAGAAT GTCCAGCAAA TGATGCAGTG TCTGATCCGC 
AAGCTTGCTG CGCTCAGCCA GTACGAAGAA GTCCTGGCAA AAATCAGCTC CACATCCACA 
GATCGGCTCA CAGTTCTCAA GACCAAGCCA CAGTCTATAC AAAGGGATAT CATTACTGTC 
TGCAACGACC CTTACACGTT GGCCCAGCAG CTGACTCATA TAGAGCTGGA GAGGCTCAAT 
TATATTGGGC CAGAAGAATT TGTTCAGGCG TTCGTGCAGA AGGACCCTTT GGATAATGAC 
AAGAGTTGCT ACAGTGAACG GAAGAAAACA CGAAACTTAG AAGCTTACGT GGAATGGTTT 
AATCGCCTCA GCTACTTGGT TGCTACAGAA ATCTGTATGC CTGTTAAGAA AAAACACCGA 
GCAAGAATGA TTGAGTATTT CATTGACGTA GCTCGGGAGT GTTTTAACAT TGGCAACTTC 
AACTCCTTGA TGGCGATAAT CTCTGGTATG AATATGAGCC CAGTCTCTCG ACTAAAAAAA 
ACTTGGGCCA AAGTGAAGAC TGCAAAATTT GACATTCTTG AGCATCAGAT GGACCCTTCA 
AGCAATTTCT ATAATTATCG AACAGCTCTT CGTGGGGCAG CACAAAGGTC TTTAACTGCT 
CATAGTAGTA GAGAAAAGAT TGTGATACCA TTCTTCAGTC TCTTAATCAA AGATATTTAT 
TTCCTCAATG AGGGTTGTGC CAACCGCCTT CCCAATGGCC ATGTCAATTT TGAGAAATTT 
TGGGAACTGG CCAAACAAGT GAGTGAATTT ATGACATGGA AACAAGTGGA GTGTCCATTT 
GAGAGGGACC GGAAGATCTT GCAGTATCTG CTCACAGTAC CAGTCTTCAG TGAAGATGCT 
CTCTACTTGG CTTCTTATGA GAGTGAAGGA CCTGAAAATC ATATAGAGAA AGACAGATGG 
AAGTCTTTAA GGTCGAGCCT CTTAGGCAGA GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGEF1B ( XM_017007813.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863302.1 Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant X2, mRNA.

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CloneID	OHu06834
Clone ID Related Accession (Same CDS sequence)	NM_152545.2 , XM_017007813.1 , NM_152545.3
Accession Version	XM_017007813.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ras-GEF domain-containing family member 1B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT 
GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAGA 
ACATACATAT TTACCTTCCT ACTCAGTTCT CGGTTATTTA TGCATCCGTA TGAGCTAATG 
GCCAAAGTTT GCCACTTATG TGTTGAGCAC CAGAGACTAA GTGATCCTGA TAGTGATAAG 
AACCAGATGA GAAAAATTGC ACCCAAAATC CTTCAACTCC TCACGGAATG GACGGAAACA 
TTTCCCTATG ATTTTCGGGA TGAAAGAATG ATGAGAAACT TAAAAGATCT GGCTCACCGA 
ATAGCCAGTG GCGAAGAGCA GACATACAGA AAGAATGTCC AGCAAATGAT GCAGTGTCTG 
ATCCGCAAGC TTGCTGCGCT CAGCCAGTAC GAAGAAGTCC TGGCAAAAAT CAGCTCCACA 
TCCACAGATC GGCTCACAGT TCTCAAGACC AAGCCACAGT CTATACAAAG GGATATCATT 
ACTGTCTGCA ACGACCCTTA CACGTTGGCC CAGCAGCTGA CTCATATAGA GCTGGAGAGG 
CTCAATTATA TTGGGCCAGA AGAATTTGTT CAGGCGTTCG TGCAGAAGGA CCCTTTGGAT 
AATGACAAGA GTTGCTACAG TGAACGGAAG AAAACACGAA ACTTAGAAGC TTACGTGGAA 
TGGTTTAATC GCCTCAGCTA CTTGGTTGCT ACAGAAATCT GTATGCCTGT TAAGAAAAAA 
CACCGAGCAA GAATGATTGA GTATTTCATT GACGTAGCTC GGGAGTGTTT TAACATTGGC 
AACTTCAACT CCTTGATGGC GATAATCTCT GGTATGAATA TGAGCCCAGT CTCTCGACTA 
AAAAAAACTT GGGCCAAAGT GAAGACTGCA AAATTTGACA TTCTTGAGCA TCAGATGGAC 
CCTTCAAGCA ATTTCTATAA TTATCGAACA GCTCTTCGTG GGGCAGCACA AAGGTCTTTA 
ACTGCTCATA GTAGTAGAGA AAAGATTGTG ATACCATTCT TCAGTCTCTT AATCAAAGAT 
ATTTATTTCC TCAATGAGGG TTGTGCCAAC CGCCTTCCCA ATGGCCATGT CAATTTTGAG 
AAATTTTGGG AACTGGCCAA ACAAGTGAGT GAATTTATGA CATGGAAACA AGTGGAGTGT 
CCATTTGAGA GGGACCGGAA GATCTTGCAG TATCTGCTCA CAGTACCAGT CTTCAGTGAA 
GATGCTCTCT ACTTGGCTTC TTATGAGAGT GAAGGACCTG AAAATCATAT AGAGAAAGAC 
AGATGGAAGT CTTTAAGGTC GAGCCTCTTA GGCAGAGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863302.1
CDS	254..1675
Translation	MPQTPPFSAMFDSSGYNRNLYQSAEDSCGGLYYHDNNLLSGSLEALIQHLVPNVDYYPDRTYIFTFLLSSRLFMHPYELMAKVCHLCVEHQRLSDPDSDKNQMRKIAPKILQLLTEWTETFPYDFRDERMMRNLKDLAHRIASGEEQTYRKNVQQMMQCLIRKLAALSQYEEVLAKISSTSTDRLTVLKTKPQSIQRDIITVCNDPYTLAQQLTHIELERLNYIGPEEFVQAFVQKDPLDNDKSCYSERKKTRNLEAYVEWFNRLSYLVATEICMPVKKKHRARMIEYFIDVARECFNIGNFNSLMAIISGMNMSPVSRLKKTWAKVKTAKFDILEHQMDPSSNFYNYRTALRGAAQRSLTAHSSREKIVIPFFSLLIKDIYFLNEGCANRLPNGHVNFEKFWELAKQVSEFMTWKQVECPFERDRKILQYLLTVPVFSEDALYLASYESEGPENHIEKDRWKSLRSSLLGRV

Target ORF information:

RefSeq Version	XM_017007813.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007813.1

ORF Insert Sequence:

ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT 
GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAGA 
ACATACATAT TTACCTTCCT ACTCAGTTCT CGGTTATTTA TGCATCCGTA TGAGCTAATG 
GCCAAAGTTT GCCACTTATG TGTTGAGCAC CAGAGACTAA GTGATCCTGA TAGTGATAAG 
AACCAGATGA GAAAAATTGC ACCCAAAATC CTTCAACTCC TCACGGAATG GACGGAAACA 
TTTCCCTATG ATTTTCGGGA TGAAAGAATG ATGAGAAACT TAAAAGATCT GGCTCACCGA 
ATAGCCAGTG GCGAAGAGCA GACATACAGA AAGAATGTCC AGCAAATGAT GCAGTGTCTG 
ATCCGCAAGC TTGCTGCGCT CAGCCAGTAC GAAGAAGTCC TGGCAAAAAT CAGCTCCACA 
TCCACAGATC GGCTCACAGT TCTCAAGACC AAGCCACAGT CTATACAAAG GGATATCATT 
ACTGTCTGCA ACGACCCTTA CACGTTGGCC CAGCAGCTGA CTCATATAGA GCTGGAGAGG 
CTCAATTATA TTGGGCCAGA AGAATTTGTT CAGGCGTTCG TGCAGAAGGA CCCTTTGGAT 
AATGACAAGA GTTGCTACAG TGAACGGAAG AAAACACGAA ACTTAGAAGC TTACGTGGAA 
TGGTTTAATC GCCTCAGCTA CTTGGTTGCT ACAGAAATCT GTATGCCTGT TAAGAAAAAA 
CACCGAGCAA GAATGATTGA GTATTTCATT GACGTAGCTC GGGAGTGTTT TAACATTGGC 
AACTTCAACT CCTTGATGGC GATAATCTCT GGTATGAATA TGAGCCCAGT CTCTCGACTA 
AAAAAAACTT GGGCCAAAGT GAAGACTGCA AAATTTGACA TTCTTGAGCA TCAGATGGAC 
CCTTCAAGCA ATTTCTATAA TTATCGAACA GCTCTTCGTG GGGCAGCACA AAGGTCTTTA 
ACTGCTCATA GTAGTAGAGA AAAGATTGTG ATACCATTCT TCAGTCTCTT AATCAAAGAT 
ATTTATTTCC TCAATGAGGG TTGTGCCAAC CGCCTTCCCA ATGGCCATGT CAATTTTGAG 
AAATTTTGGG AACTGGCCAA ACAAGTGAGT GAATTTATGA CATGGAAACA AGTGGAGTGT 
CCATTTGAGA GGGACCGGAA GATCTTGCAG TATCTGCTCA CAGTACCAGT CTTCAGTGAA 
GATGCTCTCT ACTTGGCTTC TTATGAGAGT GAAGGACCTG AAAATCATAT AGAGAAAGAC 
AGATGGAAGT CTTTAAGGTC GAGCCTCTTA GGCAGAGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGEF1B ( XM_017007812.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863301.1 Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu38733
Clone ID Related Accession (Same CDS sequence)	XM_017007812.1
Accession Version	XM_017007812.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ras-GEF domain-containing family member 1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT 
GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAAC 
CAGATGAGAA AAATTGCACC CAAAATCCTT CAACTCCTCA CGGAATGGAC GGAAACATTT 
CCCTATGATT TTCGGGATGA AAGAATGATG AGAAACTTAA AAGATCTGGC TCACCGAATA 
GCCAGTGGCG AAGAGCAGAC ATACAGAAAG AATGTCCAGC AAATGATGCA GTGTCTGATC 
CGCAAGCTTG CTGCGCTCAG CCAGTACGAA GAAGTCCTGG CAAAAATCAG CTCCACATCC 
ACAGATCGGC TCACAGTTCT CAAGACCAAG CCACAGTCTA TACAAAGGGA TATCATTACT 
GTCTGCAACG ACCCTTACAC GTTGGCCCAG CAGCTGACTC ATATAGAGCT GGAGAGGCTC 
AATTATATTG GGCCAGAAGA ATTTGTTCAG GCGTTCGTGC AGAAGGACCC TTTGGATAAT 
GACAAGAGTT GCTACAGTGA ACGGAAGAAA ACACGAAACT TAGAAGCTTA CGTGGAATGG 
TTTAATCGCC TCAGCTACTT GGTTGCTACA GAAATCTGTA TGCCTGTTAA GAAAAAACAC 
CGAGCAAGAA TGATTGAGTA TTTCATTGAC GTAGCTCGGG AGTGTTTTAA CATTGGCAAC 
TTCAACTCCT TGATGGCGAT AATCTCTGGT ATGAATATGA GCCCAGTCTC TCGACTAAAA 
AAAACTTGGG CCAAAGTGAA GACTGCAAAA TTTGACATTC TTGAGCATCA GATGGACCCT 
TCAAGCAATT TCTATAATTA TCGAACAGCT CTTCGTGGGG CAGCACAAAG GTCTTTAACT 
GCTCATAGTA GTAGAGAAAA GATTGTGATA CCATTCTTCA GTCTCTTAAT CAAAGATATT 
TATTTCCTCA ATGAGGGTTG TGCCAACCGC CTTCCCAATG GCCATGTCAA TTTTGAGAAA 
TTTTGGGAAC TGGCCAAACA AGTGAGTGAA TTTATGACAT GGAAACAAGT GGAGTGTCCA 
TTTGAGAGGG ACCGGAAGAT CTTGCAGTAT CTGCTCACAG TACCAGTCTT CAGTGAAGAT 
GCTCTCTACT TGGCTTCTTA TGAGAGTGAA GGACCTGAAA ATCATATAGA GAAAGACAGA 
TGGAAGTCTT TAAGGTCGAG CCTCTTAGGC AGAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863301.1
CDS	7..1305
Translation	MPQTPPFSAMFDSSGYNRNLYQSAEDSCGGLYYHDNNLLSGSLEALIQHLVPNVDYYPDNQMRKIAPKILQLLTEWTETFPYDFRDERMMRNLKDLAHRIASGEEQTYRKNVQQMMQCLIRKLAALSQYEEVLAKISSTSTDRLTVLKTKPQSIQRDIITVCNDPYTLAQQLTHIELERLNYIGPEEFVQAFVQKDPLDNDKSCYSERKKTRNLEAYVEWFNRLSYLVATEICMPVKKKHRARMIEYFIDVARECFNIGNFNSLMAIISGMNMSPVSRLKKTWAKVKTAKFDILEHQMDPSSNFYNYRTALRGAAQRSLTAHSSREKIVIPFFSLLIKDIYFLNEGCANRLPNGHVNFEKFWELAKQVSEFMTWKQVECPFERDRKILQYLLTVPVFSEDALYLASYESEGPENHIEKDRWKSLRSSLLGRV

Target ORF information:

RefSeq Version	XM_017007812.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007812.1

ORF Insert Sequence:

ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT 
GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAAC 
CAGATGAGAA AAATTGCACC CAAAATCCTT CAACTCCTCA CGGAATGGAC GGAAACATTT 
CCCTATGATT TTCGGGATGA AAGAATGATG AGAAACTTAA AAGATCTGGC TCACCGAATA 
GCCAGTGGCG AAGAGCAGAC ATACAGAAAG AATGTCCAGC AAATGATGCA GTGTCTGATC 
CGCAAGCTTG CTGCGCTCAG CCAGTACGAA GAAGTCCTGG CAAAAATCAG CTCCACATCC 
ACAGATCGGC TCACAGTTCT CAAGACCAAG CCACAGTCTA TACAAAGGGA TATCATTACT 
GTCTGCAACG ACCCTTACAC GTTGGCCCAG CAGCTGACTC ATATAGAGCT GGAGAGGCTC 
AATTATATTG GGCCAGAAGA ATTTGTTCAG GCGTTCGTGC AGAAGGACCC TTTGGATAAT 
GACAAGAGTT GCTACAGTGA ACGGAAGAAA ACACGAAACT TAGAAGCTTA CGTGGAATGG 
TTTAATCGCC TCAGCTACTT GGTTGCTACA GAAATCTGTA TGCCTGTTAA GAAAAAACAC 
CGAGCAAGAA TGATTGAGTA TTTCATTGAC GTAGCTCGGG AGTGTTTTAA CATTGGCAAC 
TTCAACTCCT TGATGGCGAT AATCTCTGGT ATGAATATGA GCCCAGTCTC TCGACTAAAA 
AAAACTTGGG CCAAAGTGAA GACTGCAAAA TTTGACATTC TTGAGCATCA GATGGACCCT 
TCAAGCAATT TCTATAATTA TCGAACAGCT CTTCGTGGGG CAGCACAAAG GTCTTTAACT 
GCTCATAGTA GTAGAGAAAA GATTGTGATA CCATTCTTCA GTCTCTTAAT CAAAGATATT 
TATTTCCTCA ATGAGGGTTG TGCCAACCGC CTTCCCAATG GCCATGTCAA TTTTGAGAAA 
TTTTGGGAAC TGGCCAAACA AGTGAGTGAA TTTATGACAT GGAAACAAGT GGAGTGTCCA 
TTTGAGAGGG ACCGGAAGAT CTTGCAGTAT CTGCTCACAG TACCAGTCTT CAGTGAAGAT 
GCTCTCTACT TGGCTTCTTA TGAGAGTGAA GGACCTGAAA ATCATATAGA GAAAGACAGA 
TGGAAGTCTT TAAGGTCGAG CCTCTTAGGC AGAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inducible RasGEF1B circular RNA is a positive regulator of ICAM-1 in the TLR4/LPS pathway.
RNA biology13(9)861-71(2016 09)
Ng WL,Marinov GK,Liau ES,Lam YL,Lim YY,Ea CK

Meta-analysis of genome-wide association studies of adult height in East Asians identifies 17 novel loci.
Human molecular genetics24(6)1791-800(2015 Mar)
He M,Xu M,Zhang B,Liang J,Chen P,Lee JY,Johnson TA,Li H,Yang X,Dai J,Liang L,Gui L,Qi Q,Huang J,Li Y,Adair LS,Aung T,Cai Q,Cheng CY,Cho MC,Cho YS,Chu M,Cui B,Gao YT,Go MJ,Gu D,Gu W,Guo H,Hao Y,Hong J,Hu Z,Hu Y,Huang J,Hwang JY,Ikram MK,Jin G,Kang DH,Khor CC,Kim BJ,Kim HT,Kubo M,Lee J,Lee J,Lee NR,Li R,Li J,Liu J,Longe J,Lu W,Lu X,Miao X,Okada Y,Ong RT,Qiu G,Seielstad M,Sim X,Song H,Takeuchi F,Tanaka T,Taylor PR,Wang L,Wang W,Wang Y,Wu C,Wu Y,Xiang YB,Yamamoto K,Yang H,Liao M,Yokota M,Young T,Zhang X,Kato N,Wang QK,Zheng W,Hu FB,Lin D,Shen H,Teo YY,Mo Z,Wong TY,Lin X,Mohlke KL,Ning G,Tsunoda T,Han BG,Shu XO,Tai ES,Wu T,Qi L

Common genetic variation and antidepressant efficacy in major depressive disorder: a meta-analysis of three genome-wide pharmacogenetic studies.
The American journal of psychiatry170(2)207-17(2013 Feb)
,,

8.6Mb interstitial deletion of chromosome 4q13.3q21.23 in a boy with cognitive impairment, short stature, hearing loss, skeletal abnormalities and facial dysmorphism.
Genetic counseling (Geneva, Switzerland)22(4)353-63(2011)
Lipska BS,Brzeskwiniewicz M,Wierzba J,Morzuchi L,Piotrowski A,Limon J

RasGEF1A and RasGEF1B are guanine nucleotide exchange factors that discriminate between Rap GTP-binding proteins and mediate Rap2-specific nucleotide exchange.
The FEBS journal276(16)4607-16(2009 Aug)
Yaman E,Gasper R,Koerner C,Wittinghofer A,Tazebay UH

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RASGEF1B cDNA ORF clone, Homo sapiens(human)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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