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CYBB cDNA ORF clone, Homo sapiens(Human)

Gene Symbol CYBB
Entrez Gene ID 1536
Full Name cytochrome b-245 beta chain
Synonyms AMCBX2,CGD,GP91-1,GP91-PHOX,GP91PHOX,IMD34,NOX2,p91-PHOX
General protein information
Preferred Names

cytochrome b-245 beta chain

Names

cytochrome b-245 heavy chain
CGD91-phox
NADPH oxidase 2
cytochrome b(558) subunit beta
cytochrome b-245 beta polypeptide
cytochrome b558 subunit beta
heme-binding membrane glycoprotein gp91phox
neutrophil cytochrome b 91 kDa polypeptide
p22 phagocyte B-cytochrome
superoxide-generating NADPH oxidase heavy chain subunit

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

X

Xp21.1-p11.4

Summary Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008].
Disorder MIM:

300481

Disorder Html: Chronic granulomatous disease, X-linked, 306400 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000397.3 NP_000388.2 cytochrome b-245 heavy chain



Homo sapiens (human) CYBB NP_000388.2
Bos taurus (cattle) CYBB NP_776460.1
Danio rerio (zebrafish) cybb NP_956708.1
Canis lupus familiaris (dog) CYBB NP_001093761.1
Gallus gallus (chicken) CYBB NP_001093756.1
Arabidopsis thaliana (thale cress) AT1G19230 NP_173357.1
Oryza sativa (rice) Os08g0453700 NP_001061956.1
Pan troglodytes (chimpanzee) LOC465566 XP_521002.2
Rattus norvegicus (Norway rat) Cybb NP_076455.1
Magnaporthe oryzae (rice blast fungus) MGG_06559 XP_003716981.1
Macaca mulatta (rhesus monkey) CYBB XP_001083654.1
Mus musculus (house mouse) Cybb NP_031833.3
Neurospora crassa NCU10775 XP_001728356.1
Oryza sativa (rice) Os09g0438000 NP_001063267.1
Xenopus tropicalis (tropical clawed frog) cybb NP_001025689.1


Related articles in PubMed

Atorvastatin prolongs the lifespan of radiation‑induced reactive oxygen species in PC-3 prostate cancer cells to enhance the cell killing effect.
Yu H, Sun SQ, Gu XB, Wang W, Gao XS
Oncology reports37(4)2049-2056(2017 Apr)

NOX2-ROS-HIF-1α signaling is critical for the inhibitory effect of oleanolic acid on rectal cancer cell proliferation.
Guo Y, Han B, Luo K, Ren Z, Cai L, Sun L
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie85733-739(2017 Jan)

A novel missense mutation in the NADPH binding domain of CYBB abolishes the NADPH oxidase activity in a male patient with increased susceptibility to infections.
Khan TA, Kalsoom K, Iqbal A, Asif H, Rahman H, Farooq SO, Naveed H, Nasir U, Amin MU, Hussain M, Tipu HN, Florea A
Microbial pathogenesis100163-169(2016 Nov)

Involvement of NADPH oxidases in alkali burn-induced corneal injury.
Gu XJ, Liu X, Chen YY, Zhao Y, Xu M, Han XJ, Liu QP, Yi JL, Li JM
International journal of molecular medicine38(1)75-82(2016 Jul)

Lipid peroxidation causes endosomal antigen release for cross-presentation.
Dingjan I, Verboogen DR, Paardekooper LM, Revelo NH, Sittig SP, Visser LJ, Mollard GF, Henriet SS, Figdor CG, Ter Beest M, van den Bogaart G
Scientific reports622064(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Thioredoxin attenuates oxidized low-density lipoprotein induced oxidative stress in human umbilical vein endothelial cells by reducing NOX2-NOX4 activity.
Title: Thioredoxin attenuates oxidized low-density lipoprotein induced oxidative stress in human umbilical vein endothelial cells by reducing NADPH oxidase activity.

Nox4-derived H2O2 in part activates Nox2 to increase mitochondrial ROS via pSer36-p66Shc, thereby enhancing VEGFR2 signaling and angiogenesis in endothelial cells.
Title: ROS-induced ROS release orchestrated by Nox4, Nox2, and mitochondria in VEGF signaling and angiogenesis.

NOX2, NOX4, and mitochondrial-derived reactive oxygen species contribute to angiopoietin-1 signaling and angiogenic responses in endothelial cells.
Title: NOX2, NOX4, and mitochondrial-derived reactive oxygen species contribute to angiopoietin-1 signaling and angiogenic responses in endothelial cells.

Nox4 should contribute to the pathological processes insubarachnoid hemorrhage(SAH)-induced early brain injury (EBI), and there was not an overlay effect of Nox2 inhibition and Nox4 inhibition on preventing SAH-induced EBI.
Title: Involvement of Nox2 and Nox4 NADPH oxidases in early brain injury after subarachnoid hemorrhage.

This study demonstrates that one of the critical mechanisms underlying curcumin inhibiting heat-induced apoptosis is through suppressing NADPH Oxidase 2 and activating the Akt/mTOR signaling pathway in bronchial epithelial cells.
Title: Curcumin Inhibits Heat-Induced Apoptosis by Suppressing NADPH Oxidase 2 and Activating the Akt/mTOR Signaling Pathway in Bronchial Epithelial Cells.

The following CYBB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CYBB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu27109 NM_000397.3
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Homo sapiens cytochrome b-245 beta chain (CYBB), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu27109
Clone ID Related Accession (Same CDS sequence) NM_000397.3
Accession Version NM_000397.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1713bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product cytochrome b-245 heavy chain
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA995835.1 and BC032720.1. This sequence is a reference standard in the RefSeqGene project. On Dec 21, 2007 this sequence version replaced NM_000397.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC032720.1, SRR1660809.240438.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGGGAACT GGGCTGTGAA TGAGGGGCTC TCCATTTTTG TCATTCTGGT TTGGCTGGGG 
TTGAACGTCT TCCTCTTTGT CTGGTATTAC CGGGTTTATG ATATTCCACC TAAGTTCTTT
TACACAAGAA AACTTCTTGG GTCAGCACTG GCACTGGCCA GGGCCCCTGC AGCCTGCCTG
AATTTCAACT GCATGCTGAT TCTCTTGCCA GTCTGTCGAA ATCTGCTGTC CTTCCTCAGG
GGTTCCAGTG CGTGCTGCTC AACAAGAGTT CGAAGACAAC TGGACAGGAA TCTCACCTTT
CATAAAATGG TGGCATGGAT GATTGCACTT CACTCTGCGA TTCACACCAT TGCACATCTA
TTTAATGTGG AATGGTGTGT GAATGCCCGA GTCAATAATT CTGATCCTTA TTCAGTAGCA
CTCTCTGAAC TTGGAGACAG GCAAAATGAA AGTTATCTCA ATTTTGCTCG AAAGAGAATA
AAGAACCCTG AAGGAGGCCT GTACCTGGCT GTGACCCTGT TGGCAGGCAT CACTGGAGTT
GTCATCACGC TGTGCCTCAT ATTAATTATC ACTTCCTCCA CCAAAACCAT CCGGAGGTCT
TACTTTGAAG TCTTTTGGTA CACACATCAT CTCTTTGTGA TCTTCTTCAT TGGCCTTGCC
ATCCATGGAG CTGAACGAAT TGTACGTGGG CAGACCGCAG AGAGTTTGGC TGTGCATAAT
ATAACAGTTT GTGAACAAAA AATCTCAGAA TGGGGAAAAA TAAAGGAATG CCCAATCCCT
CAGTTTGCTG GAAACCCTCC TATGACTTGG AAATGGATAG TGGGTCCCAT GTTTCTGTAT
CTCTGTGAGA GGTTGGTGCG GTTTTGGCGA TCTCAACAGA AGGTGGTCAT CACCAAGGTG
GTCACTCACC CTTTCAAAAC CATCGAGCTA CAGATGAAGA AGAAGGGGTT CAAAATGGAA
GTGGGACAAT ACATTTTTGT CAAGTGCCCA AAGGTGTCCA AGCTGGAGTG GCACCCTTTT
ACACTGACAT CCGCCCCTGA GGAAGACTTC TTTAGTATCC ATATCCGCAT CGTTGGGGAC
TGGACAGAGG GGCTGTTCAA TGCTTGTGGC TGTGATAAGC AGGAGTTTCA AGATGCGTGG
AAACTACCTA AGATAGCGGT TGATGGGCCC TTTGGCACTG CCAGTGAAGA TGTGTTCAGC
TATGAGGTGG TGATGTTAGT GGGAGCAGGG ATTGGGGTCA CACCCTTCGC ATCCATTCTC
AAGTCAGTCT GGTACAAATA TTGCAATAAC GCCACCAATC TGAAGCTCAA AAAGATCTAC
TTCTACTGGC TGTGCCGGGA CACACATGCC TTTGAGTGGT TTGCAGATCT GCTGCAACTG
CTGGAGAGCC AGATGCAGGA AAGGAACAAT GCCGGCTTCC TCAGCTACAA CATCTACCTC
ACTGGCTGGG ATGAGTCTCA GGCCAATCAC TTTGCTGTGC ACCATGATGA GGAGAAAGAT
GTGATCACAG GCCTGAAACA AAAGACTTTG TATGGACGGC CCAACTGGGA TAATGAATTC
AAGACAATTG CAAGTCAACA CCCTAATACC AGAATAGGAG TTTTCCTCTG TGGACCTGAA
GCCTTGGCTG AAACCCTGAG TAAACAAAGC ATCTCCAACT CTGAGTCTGG CCCTCGGGGA
GTGCATTTCA TTTTCAACAA GGAAAACTTC TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000388.2
CDS62..1774
Misc Feature(1)17..19
Misc Feature(2)86..148
Misc Feature(3)254..718
Misc Feature(4)263..265
Misc Feature(5)314..316
Misc Feature(6)368..430
Misc Feature(7)569..631
Misc Feature(8)662..724
Misc Feature(9)845..907
Misc Feature(10)950..1771
Misc Feature(11)order(1031..1033,1073..1084,1121..1129,1136..1147,1292..1294)
Misc Feature(12)order(1073..1075,1079..1084)
Misc Feature(13)1277..1309
Misc Feature(14)order(1277..1279,1289..1300,1304..1306)
Misc Feature(15)order(1292..1297,1391..1399,1673..1678)
Misc Feature(16)order(1385..1390,1397..1399)
Exon (1)1..106
Exon (2)107..202
Exon (3)203..313
Exon (4)314..398
Exon (5)399..544
Exon (6)545..735
Exon (7)736..865
Exon (8)866..958
Exon (9)959..1212
Exon (10)1213..1375
Exon (11)1376..1522
Exon (12)1523..1647
Exon (13)1648..4318
Translation

Target ORF information:

RefSeq Version NM_000397.3
Organism Homo sapiens(Human)
Definition Homo sapiens cytochrome b-245 beta chain (CYBB), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000397.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGGGAACT GGGCTGTGAA TGAGGGGCTC TCCATTTTTG TCATTCTGGT TTGGCTGGGG 
TTGAACGTCT TCCTCTTTGT CTGGTATTAC CGGGTTTATG ATATTCCACC TAAGTTCTTT
TACACAAGAA AACTTCTTGG GTCAGCACTG GCACTGGCCA GGGCCCCTGC AGCCTGCCTG
AATTTCAACT GCATGCTGAT TCTCTTGCCA GTCTGTCGAA ATCTGCTGTC CTTCCTCAGG
GGTTCCAGTG CGTGCTGCTC AACAAGAGTT CGAAGACAAC TGGACAGGAA TCTCACCTTT
CATAAAATGG TGGCATGGAT GATTGCACTT CACTCTGCGA TTCACACCAT TGCACATCTA
TTTAATGTGG AATGGTGTGT GAATGCCCGA GTCAATAATT CTGATCCTTA TTCAGTAGCA
CTCTCTGAAC TTGGAGACAG GCAAAATGAA AGTTATCTCA ATTTTGCTCG AAAGAGAATA
AAGAACCCTG AAGGAGGCCT GTACCTGGCT GTGACCCTGT TGGCAGGCAT CACTGGAGTT
GTCATCACGC TGTGCCTCAT ATTAATTATC ACTTCCTCCA CCAAAACCAT CCGGAGGTCT
TACTTTGAAG TCTTTTGGTA CACACATCAT CTCTTTGTGA TCTTCTTCAT TGGCCTTGCC
ATCCATGGAG CTGAACGAAT TGTACGTGGG CAGACCGCAG AGAGTTTGGC TGTGCATAAT
ATAACAGTTT GTGAACAAAA AATCTCAGAA TGGGGAAAAA TAAAGGAATG CCCAATCCCT
CAGTTTGCTG GAAACCCTCC TATGACTTGG AAATGGATAG TGGGTCCCAT GTTTCTGTAT
CTCTGTGAGA GGTTGGTGCG GTTTTGGCGA TCTCAACAGA AGGTGGTCAT CACCAAGGTG
GTCACTCACC CTTTCAAAAC CATCGAGCTA CAGATGAAGA AGAAGGGGTT CAAAATGGAA
GTGGGACAAT ACATTTTTGT CAAGTGCCCA AAGGTGTCCA AGCTGGAGTG GCACCCTTTT
ACACTGACAT CCGCCCCTGA GGAAGACTTC TTTAGTATCC ATATCCGCAT CGTTGGGGAC
TGGACAGAGG GGCTGTTCAA TGCTTGTGGC TGTGATAAGC AGGAGTTTCA AGATGCGTGG
AAACTACCTA AGATAGCGGT TGATGGGCCC TTTGGCACTG CCAGTGAAGA TGTGTTCAGC
TATGAGGTGG TGATGTTAGT GGGAGCAGGG ATTGGGGTCA CACCCTTCGC ATCCATTCTC
AAGTCAGTCT GGTACAAATA TTGCAATAAC GCCACCAATC TGAAGCTCAA AAAGATCTAC
TTCTACTGGC TGTGCCGGGA CACACATGCC TTTGAGTGGT TTGCAGATCT GCTGCAACTG
CTGGAGAGCC AGATGCAGGA AAGGAACAAT GCCGGCTTCC TCAGCTACAA CATCTACCTC
ACTGGCTGGG ATGAGTCTCA GGCCAATCAC TTTGCTGTGC ACCATGATGA GGAGAAAGAT
GTGATCACAG GCCTGAAACA AAAGACTTTG TATGGACGGC CCAACTGGGA TAATGAATTC
AAGACAATTG CAAGTCAACA CCCTAATACC AGAATAGGAG TTTTCCTCTG TGGACCTGAA
GCCTTGGCTG AAACCCTGAG TAAACAAAGC ATCTCCAACT CTGAGTCTGG CCCTCGGGGA
GTGCATTTCA TTTTCAACAA GGAAAACTTC TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Atorvastatin prolongs the lifespan of radiation‑induced reactive oxygen species in PC-3 prostate cancer cells to enhance the cell killing effect.
Oncology reports37(4)2049-2056(2017 Apr)
Yu H,Sun SQ,Gu XB,Wang W,Gao XS


book

NOX2-ROS-HIF-1α signaling is critical for the inhibitory effect of oleanolic acid on rectal cancer cell proliferation.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie85733-739(2017 Jan)
Guo Y,Han B,Luo K,Ren Z,Cai L,Sun L


book

A novel missense mutation in the NADPH binding domain of CYBB abolishes the NADPH oxidase activity in a male patient with increased susceptibility to infections.
Microbial pathogenesis100163-169(2016 Nov)
Khan TA,Kalsoom K,Iqbal A,Asif H,Rahman H,Farooq SO,Naveed H,Nasir U,Amin MU,Hussain M,Tipu HN,Florea A


book

Involvement of NADPH oxidases in alkali burn-induced corneal injury.
International journal of molecular medicine38(1)75-82(2016 Jul)
Gu XJ,Liu X,Chen YY,Zhao Y,Xu M,Han XJ,Liu QP,Yi JL,Li JM


book

Lipid peroxidation causes endosomal antigen release for cross-presentation.
Scientific reports622064(2016 Feb)
Dingjan I,Verboogen DR,Paardekooper LM,Revelo NH,Sittig SP,Visser LJ,Mollard GF,Henriet SS,Figdor CG,Ter Beest M,van den Bogaart G


 
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