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CYP2E1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol CYP2E1
Entrez Gene ID 1571
Full Name cytochrome P450, family 2, subfamily E, polypeptide 1
Synonyms CPE1, CYP2E, P450-J, P450C2E
General protein information
Preferred Names
cytochrome P450 2E1
Names
cytochrome P450 2E1
CYPIIE1
cytochrome P450-J
microsomal monooxygenase
xenobiotic monooxygenase
4-nitrophenol 2-hydroxylase
flavoprotein-linked monooxygenase
cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1
NP_000764.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

10

10q26.3

Summary This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

124040


mRNA and Protein(s)

mRNA Protein Name
NM_000773 NP_000764 cytochrome P450 2E1 precursor



Homo sapiens (human) CYP2E1 NP_000764.1
Pan troglodytes (chimpanzee) LOC450857 XP_508139.3
Macaca mulatta (Rhesus monkey) CYP2E1 NP_001035303.1
Canis lupus familiaris (dog) CYP2E1 NP_001003339.1
Bos taurus (cattle) CYP2E1 NP_776955.2
Mus musculus (house mouse) Cyp2e1 NP_067257.1
Rattus norvegicus (Norway rat) Cyp2e1 NP_113731.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CYP2E1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CYP2E1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu25425
NM_000773 Homo sapiens cytochrome P450, family 2, subfamily E, polypeptide 1 (CYP2E1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$204.50-$286.30
$409.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu25425
Clone ID Related Accession (Same CDS sequence) NM_000773
Accession Version NM_000773.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cytochrome P450 2E1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX951478.1, J02625.1 and AL161645.14. This sequence is a reference standard in the RefSeqGene project. On Sep 15, 2005 this sequence version replaced gi:13699837. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J02625.1, AF182276.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTCTGCCC TCGGAGTCAC CGTGGCCCTG CTGGTGTGGG CGGCCTTCCT CCTGCTGGTG 
TCCATGTGGA GGCAGGTGCA CAGCAGCTGG AATCTGCCCC CAGGCCCTTT CCCGCTTCCC
ATCATCGGGA ACCTCTTCCA GTTGGAATTG AAGAATATTC CCAAGTCCTT CACCCGGTTG
GCCCAGCGCT TCGGGCCGGT GTTCACGCTG TACGTGGGCT CGCAGCGCAT GGTGGTGATG
CACGGCTACA AGGCGGTGAA GGAAGCGCTG CTGGACTACA AGGACGAGTT CTCGGGCAGA
GGCGACCTCC CCGCGTTCCA TGCGCACAGG GACAGGGGAA TCATTTTTAA TAATGGACCT
ACCTGGAAGG ACATCCGGCG GTTTTCCCTG ACCACCCTCC GGAACTATGG GATGGGGAAA
CAGGGCAATG AGAGCCGGAT CCAGAGGGAG GCCCACTTCC TGCTGGAAGC ACTCAGGAAG
ACCCAAGGCC AGCCTTTCGA CCCCACCTTC CTCATCGGCT GCGCGCCCTG CAACGTCATA
GCCGACATCC TCTTCCGCAA GCATTTTGAC TACAATGATG AGAAGTTTCT AAGGCTGATG
TATTTGTTTA ATGAGAACTT CCACCTACTC AGCACTCCCT GGCTCCAGCT TTACAATAAT
TTTCCCAGCT TTCTACACTA CTTGCCTGGA AGCCACAGAA AAGTCATAAA AAATGTGGCT
GAAGTAAAAG AGTATGTGTC TGAAAGGGTG AAGGAGCACC ATCAATCTCT GGACCCCAAC
TGTCCCCGGG ACCTCACCGA CTGCCTGCTC GTGGAAATGG AGAAGGAAAA GCACAGTGCA
GAGCGCTTGT ACACAATGGA CGGTATCACC GTGACTGTGG CCGACCTGTT CTTTGCGGGG
ACAGAGACCA CCAGCACAAC TCTGAGATAT GGGCTCCTGA TTCTCATGAA ATACCCTGAG
ATCGAAGAGA AGCTCCATGA AGAAATTGAC AGGGTGATTG GGCCAAGCCG AATCCCTGCC
ATCAAGGATA GGCAAGAGAT GCCCTACATG GATGCTGTGG TGCATGAGAT TCAGCGGTTC
ATCACCCTCG TGCCCTCCAA CCTGCCCCAT GAAGCAACCC GAGACACCAT TTTCAGAGGA
TACCTCATCC CCAAGGGCAC AGTCGTAGTG CCAACTCTGG ACTCTGTTTT GTATGACAAC
CAAGAATTTC CTGATCCAGA AAAGTTTAAG CCAGAACACT TCCTGAATGA AAATGGAAAG
TTCAAGTACA GTGACTATTT CAAGCCATTT TCCACAGGAA AACGAGTGTG TGCTGGAGAA
GGCCTGGCTC GCATGGAGTT GTTTCTTTTG TTGTGTGCCA TTTTGCAGCA TTTTAATTTG
AAGCCTCTCG TTGACCCAAA GGATATCGAC CTCAGCCCTA TACATATTGG GTTTGGCTGT
ATCCCACCAC GTTACAAACT CTGTGTCATT CCCCGCTCAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000764.1
CDS34..1515
Misc Feature(1)130..1500(+)
Misc Feature(2)925..942(+)
Misc Feature(3)1342..1344(+)
Exon (1)1..210
Gene:CYP2E1
Gene Synonym:
Exon (2)211..370
Gene:CYP2E1
Gene Synonym:
Exon (3)371..520
Gene:CYP2E1
Gene Synonym:
Exon (4)521..681
Gene:CYP2E1
Gene Synonym:
Exon (5)682..858
Gene:CYP2E1
Gene Synonym:
Exon (6)859..1000
Gene:CYP2E1
Gene Synonym:
Exon (7)1001..1188
Gene:CYP2E1
Gene Synonym:
Exon (8)1189..1330
Gene:CYP2E1
Gene Synonym:
Exon (9)1331..1667
Gene:CYP2E1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000773
Organism Homo sapiens (human)
Definition Homo sapiens cytochrome P450, family 2, subfamily E, polypeptide 1 (CYP2E1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000773

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTCTGCCC TCGGAGTCAC CGTGGCCCTG CTGGTGTGGG CGGCCTTCCT CCTGCTGGTG 
TCCATGTGGA GGCAGGTGCA CAGCAGCTGG AATCTGCCCC CAGGCCCTTT CCCGCTTCCC
ATCATCGGGA ACCTCTTCCA GTTGGAATTG AAGAATATTC CCAAGTCCTT CACCCGGTTG
GCCCAGCGCT TCGGGCCGGT GTTCACGCTG TACGTGGGCT CGCAGCGCAT GGTGGTGATG
CACGGCTACA AGGCGGTGAA GGAAGCGCTG CTGGACTACA AGGACGAGTT CTCGGGCAGA
GGCGACCTCC CCGCGTTCCA TGCGCACAGG GACAGGGGAA TCATTTTTAA TAATGGACCT
ACCTGGAAGG ACATCCGGCG GTTTTCCCTG ACCACCCTCC GGAACTATGG GATGGGGAAA
CAGGGCAATG AGAGCCGGAT CCAGAGGGAG GCCCACTTCC TGCTGGAAGC ACTCAGGAAG
ACCCAAGGCC AGCCTTTCGA CCCCACCTTC CTCATCGGCT GCGCGCCCTG CAACGTCATA
GCCGACATCC TCTTCCGCAA GCATTTTGAC TACAATGATG AGAAGTTTCT AAGGCTGATG
TATTTGTTTA ATGAGAACTT CCACCTACTC AGCACTCCCT GGCTCCAGCT TTACAATAAT
TTTCCCAGCT TTCTACACTA CTTGCCTGGA AGCCACAGAA AAGTCATAAA AAATGTGGCT
GAAGTAAAAG AGTATGTGTC TGAAAGGGTG AAGGAGCACC ATCAATCTCT GGACCCCAAC
TGTCCCCGGG ACCTCACCGA CTGCCTGCTC GTGGAAATGG AGAAGGAAAA GCACAGTGCA
GAGCGCTTGT ACACAATGGA CGGTATCACC GTGACTGTGG CCGACCTGTT CTTTGCGGGG
ACAGAGACCA CCAGCACAAC TCTGAGATAT GGGCTCCTGA TTCTCATGAA ATACCCTGAG
ATCGAAGAGA AGCTCCATGA AGAAATTGAC AGGGTGATTG GGCCAAGCCG AATCCCTGCC
ATCAAGGATA GGCAAGAGAT GCCCTACATG GATGCTGTGG TGCATGAGAT TCAGCGGTTC
ATCACCCTCG TGCCCTCCAA CCTGCCCCAT GAAGCAACCC GAGACACCAT TTTCAGAGGA
TACCTCATCC CCAAGGGCAC AGTCGTAGTG CCAACTCTGG ACTCTGTTTT GTATGACAAC
CAAGAATTTC CTGATCCAGA AAAGTTTAAG CCAGAACACT TCCTGAATGA AAATGGAAAG
TTCAAGTACA GTGACTATTT CAAGCCATTT TCCACAGGAA AACGAGTGTG TGCTGGAGAA
GGCCTGGCTC GCATGGAGTT GTTTCTTTTG TTGTGTGCCA TTTTGCAGCA TTTTAATTTG
AAGCCTCTCG TTGACCCAAA GGATATCGAC CTCAGCCCTA TACATATTGG GTTTGGCTGT
ATCCCACCAC GTTACAAACT CTGTGTCATT CCCCGCTCAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Inhibitory potency of 4-carbon alkanes and alkenes toward CYP2E1 activity
Toxicology 318, 51-58 (2014)
Hartman JH, Miller GP and Boysen G.


book

Role and importance of polymorphisms with respect to DNA methylation for the expression of CYP2E1 enzyme
Gene 536 (1), 29-39 (2014)
Naselli F, Catanzaro I, Bellavia D, Perez A, Sposito L and Caradonna F.


book

A regulatory variant in CYP2E1 affects the risk of lung squamous cell carcinoma
Tumour Biol. 35 (1), 455-462 (2014)
Cao L, Lin J, He B, Wang H, Rao J, Liu Y and Zhang X.


book

[Polymorphism of ethanol metabolism genes in alcoholic chronic pancreatitis]
Ter. Arkh. 86 (2), 49-55 (2014)
Gubergrits,N.B., Kishenya,M.S. and Golubova,O.A.


book

HIV gp120- and methamphetamine-mediated oxidative stress induces astrocyte apoptosis via cytochrome P450 2E1
Cell Death Dis 4, E850 (2013)
Shah A, Kumar S, Simon SD, Singh DP and Kumar A.


book

Comparison of cytochrome P450 (CYP) genes from the mouse and human genomes, including nomenclature recommendations for genes, pseudogenes and alternative-splice variants
Pharmacogenetics 14 (1), 1-18 (2004)
Nelson DR, Zeldin DC, Hoffman SM, Maltais LJ, Wain HM and Nebert DW.


book

MspI polymorphism of the human CYP2E gene
Nucleic Acids Res. 19 (20), 5797 (1991)
Uematsu F, Kikuchi H, Abe T, Motomiya M, Ohmachi T, Sagami I and Watanabe M.


book

Genetic polymorphisms in the 5'-flanking region change transcriptional regulation of the human cytochrome P450IIE1 gene
J. Biochem. 110 (4), 559-565 (1991)
Hayashi S, Watanabe J and Kawajiri K.


book

Human liver cytochrome P-450 related to a rat acetone-inducible, nitrosamine-metabolizing cytochrome P-450: identification and isolation
Pharmacology 39 (3), 137-144 (1989)
Robinson RC, Shorr RG, Varrichio A, Park SS, Gelboin HV, Miller H and Friedman FK.


book

Human ethanol-inducible P450IIE1: complete gene sequence, promoter characterization, chromosome mapping, and cDNA-directed expression
Biochemistry 27 (25), 9006-9013 (1988)
Umeno M, McBride OW, Yang CS, Gelboin HV and Gonzalez FJ.


 
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