DGKA cDNA ORF clone, Homo sapiens(human)

The following DGKA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu02866 NM_201445.1
Latest version!
Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu02866 NM_201444.2
Latest version!
Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu02866 NM_001345.5
Latest version!
Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu02866 NM_001351034.1
Latest version!
Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu02866 NM_001351033.1
Latest version!
Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu02866 NM_201554.1
Latest version!
Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu02866 XM_011537993.2
Latest version!
Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu02866 XM_011537992.1
Latest version!
Homo sapiens diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu02866 XM_011537991.1
Latest version!
Homo sapiens diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu102466 NM_001351040.1
Latest version!
Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu102466 NM_001351039.1
Latest version!
Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu102466 NM_001351038.1
Latest version!
Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu46356 XM_005268689.2
Latest version!
Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu46356 XM_005268690.2
Latest version!
Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu46356 XM_011537995.2
Latest version!
Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu102467 NM_001351037.1
Latest version!
Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $307.30
$439.00
OHu102467 NM_001351036.1
Latest version!
Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $307.30
$439.00
OHu46355 NM_001351035.1
Latest version!
Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu83567 XM_017018900.1
Latest version!
Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu83568 XM_017018906.2
Latest version!
Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu83568 XM_017018905.1
Latest version!
Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu83568 XM_017018907.1
Latest version!
Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu83568 XM_017018901.2
Latest version!
Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu83568 XM_017018902.1
Latest version!
Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu83568 XM_017018903.1
Latest version!
Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu83568 XM_017018904.1
Latest version!
Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu83569 XM_017018908.1
Latest version!
Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OHu83568 XM_017018906.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu83568 XM_017018901.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu02866 NM_001345.4 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu02866 XM_011537993.1 Homo sapiens diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu02866
    Clone ID Related Accession (Same CDS sequence) NM_001351033.1 , NM_201445.1 , NM_201554.1 , NM_001345.4 , XM_011537992.1 , XM_011537991.1 , XM_011537993.1 , NM_001345.5 , NM_201444.2 , NM_001351034.1 , XM_011537993.2
    Accession Version NM_001351033.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-30
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025162.48 and BC031870.2. Transcript Variant: This variant (5), as well as variants 1-4 and 6, encodes isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC031870.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153031, SAMEA2156670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA
    CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT
    CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG
    GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG
    AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT
    CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC
    ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG
    TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG
    CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT
    GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC
    TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC
    TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG
    AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT
    GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA
    TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA
    TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG
    ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC
    CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG
    AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG
    TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC
    ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG
    CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA
    TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT
    GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA
    AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG
    GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT
    ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG
    GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC
    AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA
    CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC
    ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337962.1
    CDS235..2442
    Translation

    Target ORF information:

    RefSeq Version NM_001351033.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001351033.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA
    CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT
    CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG
    GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG
    AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT
    CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC
    ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG
    TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG
    CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT
    GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC
    TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC
    TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG
    AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT
    GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA
    TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA
    TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG
    ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC
    CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG
    AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG
    TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC
    ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG
    CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA
    TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT
    GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA
    AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG
    GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT
    ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG
    GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC
    AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA
    CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC
    ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02866
    Clone ID Related Accession (Same CDS sequence) NM_001351033.1 , NM_201445.1 , NM_201554.1 , NM_001345.4 , XM_011537992.1 , XM_011537991.1 , XM_011537993.1 , NM_001345.5 , NM_201444.2 , NM_001351034.1 , XM_011537993.2
    Accession Version NM_201445.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-30
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF448612.1, BC023523.2, BC031870.2 and AF064768.1. Transcript Variant: This variant (2), as well as variants 1 and 3-6, encodes isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC023523.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA
    CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT
    CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG
    GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG
    AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT
    CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC
    ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG
    TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG
    CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT
    GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC
    TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC
    TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG
    AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT
    GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA
    TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA
    TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG
    ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC
    CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG
    AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG
    TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC
    ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG
    CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA
    TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT
    GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA
    AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG
    GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT
    ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG
    GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC
    AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA
    CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC
    ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_958853.1
    CDS278..2485
    Translation

    Target ORF information:

    RefSeq Version NM_201445.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_201445.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA
    CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT
    CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG
    GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG
    AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT
    CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC
    ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG
    TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG
    CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT
    GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC
    TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC
    TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG
    AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT
    GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA
    TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA
    TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG
    ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC
    CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG
    AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG
    TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC
    ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG
    CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA
    TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT
    GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA
    AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG
    GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT
    ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG
    GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC
    AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA
    CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC
    ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02866
    Clone ID Related Accession (Same CDS sequence) NM_001351033.1 , NM_201445.1 , NM_201554.1 , NM_001345.4 , XM_011537992.1 , XM_011537991.1 , XM_011537993.1 , NM_001345.5 , NM_201444.2 , NM_001351034.1 , XM_011537993.2
    Accession Version NM_201554.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-30
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF448612.1, BC023523.2, BC031870.2 and AF064768.1. Transcript Variant: This variant (4), as well as variants 1-3, 5, and 6, encodes isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.57202.1, SRR1803611.230752.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA
    CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT
    CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG
    GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG
    AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT
    CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC
    ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG
    TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG
    CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT
    GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC
    TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC
    TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG
    AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT
    GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA
    TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA
    TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG
    ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC
    CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG
    AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG
    TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC
    ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG
    CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA
    TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT
    GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA
    AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG
    GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT
    ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG
    GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC
    AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA
    CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC
    ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_963848.1
    CDS178..2385
    Translation

    Target ORF information:

    RefSeq Version NM_201554.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_201554.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA
    CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT
    CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG
    GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG
    AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT
    CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC
    ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG
    TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG
    CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT
    GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC
    TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC
    TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG
    AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT
    GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA
    TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA
    TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG
    ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC
    CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG
    AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG
    TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC
    ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG
    CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA
    TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT
    GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA
    AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG
    GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT
    ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG
    GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC
    AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA
    CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC
    ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02866
    Clone ID Related Accession (Same CDS sequence) NM_001351033.1 , NM_201445.1 , NM_201554.1 , NM_001345.4 , XM_011537992.1 , XM_011537991.1 , XM_011537993.1 , NM_001345.5 , NM_201444.2 , NM_001351034.1 , XM_011537993.2
    Accession Version NM_001345.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-11-19
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM836118.1, BQ943057.1, BC031870.2, BC023523.2 and AF064768.1. On Jan 30, 2004 this sequence version replaced NM_001345.3. Transcript Variant: This variant (3), as well as variants 1, 2, and 4-6, encodes isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.209972.1, SRR1803612.235168.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA
    CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT
    CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG
    GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG
    AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT
    CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC
    ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG
    TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG
    CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT
    GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC
    TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC
    TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG
    AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT
    GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA
    TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA
    TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG
    ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC
    CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG
    AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG
    TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC
    ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG
    CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA
    TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT
    GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA
    AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG
    GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT
    ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG
    GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC
    AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA
    CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC
    ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336.2
    CDS265..2472
    Translation

    Target ORF information:

    RefSeq Version NM_001345.4
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001345.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA
    CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT
    CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG
    GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG
    AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT
    CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC
    ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG
    TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG
    CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT
    GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC
    TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC
    TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG
    AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT
    GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA
    TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA
    TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG
    ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC
    CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG
    AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG
    TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC
    ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG
    CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA
    TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT
    GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA
    AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG
    GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT
    ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG
    GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC
    AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA
    CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC
    ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02866
    Clone ID Related Accession (Same CDS sequence) NM_001351033.1 , NM_201445.1 , NM_201554.1 , NM_001345.4 , XM_011537992.1 , XM_011537991.1 , XM_011537993.1 , NM_001345.5 , NM_201444.2 , NM_001351034.1 , XM_011537993.2
    Accession Version NM_001345.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-30
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM836118.1, BQ943057.1, BC031870.2 and BC023523.2. On Nov 22, 2018 this sequence version replaced NM_001345.4. Transcript Variant: This variant (3), as well as variants 1, 2, and 4-6, encodes isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.209972.1, SRR1803612.235168.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000331886.10/ ENSP00000328405.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA
    CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT
    CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG
    GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG
    AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT
    CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC
    ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG
    TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG
    CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT
    GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC
    TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC
    TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG
    AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT
    GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA
    TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA
    TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG
    ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC
    CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG
    AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG
    TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC
    ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG
    CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA
    TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT
    GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA
    AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG
    GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT
    ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG
    GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC
    AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA
    CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC
    ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336.2
    CDS265..2472
    Translation

    Target ORF information:

    RefSeq Version NM_001345.5
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001345.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA
    CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT
    CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG
    GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG
    AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT
    CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC
    ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG
    TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG
    CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT
    GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC
    TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC
    TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG
    AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT
    GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA
    TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA
    TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG
    ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC
    CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG
    AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG
    TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC
    ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG
    CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA
    TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT
    GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA
    AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG
    GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT
    ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG
    GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC
    AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA
    CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC
    ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02866
    Clone ID Related Accession (Same CDS sequence) NM_001351033.1 , NM_201445.1 , NM_201554.1 , NM_001345.4 , XM_011537992.1 , XM_011537991.1 , XM_011537993.1 , NM_001345.5 , NM_201444.2 , NM_001351034.1 , XM_011537993.2
    Accession Version NM_201444.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-30
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI815678.1, BM910598.1, BC031870.2, BC023523.2 and AF064768.1. On Feb 3, 2004 this sequence version replaced NM_201444.1. Transcript Variant: This variant (1), as well as variants 2-6, encodes isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.189221.1, SRR1803612.26114.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA
    CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT
    CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG
    GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG
    AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT
    CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC
    ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG
    TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG
    CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT
    GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC
    TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC
    TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG
    AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT
    GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA
    TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA
    TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG
    ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC
    CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG
    AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG
    TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC
    ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG
    CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA
    TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT
    GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA
    AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG
    GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT
    ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG
    GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC
    AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA
    CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC
    ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_958852.1
    CDS310..2517
    Translation

    Target ORF information:

    RefSeq Version NM_201444.2
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_201444.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA
    CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT
    CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG
    GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG
    AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT
    CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC
    ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG
    TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG
    CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT
    GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC
    TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC
    TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG
    AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT
    GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA
    TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA
    TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG
    ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC
    CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG
    AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG
    TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC
    ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG
    CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA
    TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT
    GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA
    AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG
    GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT
    ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG
    GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC
    AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA
    CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC
    ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02866
    Clone ID Related Accession (Same CDS sequence) NM_001351033.1 , NM_201445.1 , NM_201554.1 , NM_001345.4 , XM_011537992.1 , XM_011537991.1 , XM_011537993.1 , NM_001345.5 , NM_201444.2 , NM_001351034.1 , XM_011537993.2
    Accession Version NM_001351034.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-30
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025162.48. On Apr 29, 2017 this sequence version replaced XM_011537991.2. Transcript Variant: This variant (6), as well as variants 1-5, encodes isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.274080.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA
    CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT
    CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG
    GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG
    AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT
    CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC
    ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG
    TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG
    CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT
    GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC
    TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC
    TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG
    AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT
    GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA
    TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA
    TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG
    ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC
    CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG
    AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG
    TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC
    ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG
    CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA
    TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT
    GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA
    AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG
    GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT
    ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG
    GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC
    AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA
    CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC
    ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337963.1
    CDS410..2617
    Translation

    Target ORF information:

    RefSeq Version NM_001351034.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001351034.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA
    CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT
    CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG
    GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG
    AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT
    CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC
    ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG
    TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG
    CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT
    GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC
    TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC
    TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG
    AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT
    GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA
    TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA
    TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG
    ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC
    CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG
    AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG
    TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC
    ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG
    CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA
    TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT
    GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA
    AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG
    GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT
    ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG
    GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC
    AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA
    CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC
    ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02866
    Clone ID Related Accession (Same CDS sequence) NM_001351033.1 , NM_201445.1 , NM_201554.1 , NM_001345.4 , XM_011537992.1 , XM_011537991.1 , XM_011537993.1 , NM_001345.5 , NM_201444.2 , NM_001351034.1 , XM_011537993.2
    Accession Version XM_011537992.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product diacylglycerol kinase alpha isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA
    CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT
    CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG
    GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG
    AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT
    CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC
    ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG
    TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG
    CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT
    GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC
    TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC
    TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG
    AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT
    GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA
    TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA
    TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG
    ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC
    CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG
    AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG
    TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC
    ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG
    CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA
    TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT
    GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA
    AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG
    GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT
    ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG
    GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC
    AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA
    CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC
    ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011536294.1
    Translation

    Target ORF information:

    RefSeq Version XM_011537992.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011537992.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA
    CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT
    CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG
    GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG
    AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT
    CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC
    ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG
    TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG
    CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT
    GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC
    TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC
    TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG
    AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT
    GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA
    TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA
    TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG
    ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC
    CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG
    AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG
    TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC
    ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG
    CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA
    TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT
    GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA
    AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG
    GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT
    ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG
    GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC
    AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA
    CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC
    ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02866
    Clone ID Related Accession (Same CDS sequence) NM_001351033.1 , NM_201445.1 , NM_201554.1 , NM_001345.4 , XM_011537992.1 , XM_011537991.1 , XM_011537993.1 , NM_001345.5 , NM_201444.2 , NM_001351034.1 , XM_011537993.2
    Accession Version XM_011537991.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product diacylglycerol kinase alpha isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA
    CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT
    CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG
    GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG
    AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT
    CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC
    ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG
    TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG
    CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT
    GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC
    TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC
    TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG
    AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT
    GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA
    TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA
    TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG
    ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC
    CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG
    AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG
    TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC
    ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG
    CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA
    TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT
    GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA
    AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG
    GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT
    ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG
    GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC
    AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA
    CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC
    ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011536293.1
    Translation

    Target ORF information:

    RefSeq Version XM_011537991.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011537991.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA
    CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT
    CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG
    GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG
    AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT
    CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC
    ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG
    TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG
    CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT
    GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC
    TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC
    TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG
    AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT
    GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA
    TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA
    TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG
    ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC
    CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG
    AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG
    TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC
    ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG
    CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA
    TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT
    GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA
    AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG
    GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT
    ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG
    GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC
    AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA
    CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC
    ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02866
    Clone ID Related Accession (Same CDS sequence) NM_001351033.1 , NM_201445.1 , NM_201554.1 , NM_001345.4 , XM_011537992.1 , XM_011537991.1 , XM_011537993.1 , NM_001345.5 , NM_201444.2 , NM_001351034.1 , XM_011537993.2
    Accession Version XM_011537993.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product diacylglycerol kinase alpha isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA
    CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT
    CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG
    GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG
    AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT
    CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC
    ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG
    TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG
    CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT
    GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC
    TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC
    TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG
    AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT
    GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA
    TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA
    TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG
    ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC
    CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG
    AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG
    TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC
    ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG
    CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA
    TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT
    GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA
    AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG
    GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT
    ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG
    GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC
    AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA
    CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC
    ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011536295.1
    Translation

    Target ORF information:

    RefSeq Version XM_011537993.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011537993.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA
    CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT
    CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG
    GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG
    AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT
    CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC
    ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG
    TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG
    CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT
    GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC
    TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC
    TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG
    AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT
    GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA
    TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA
    TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG
    ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC
    CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG
    AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG
    TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC
    ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG
    CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA
    TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT
    GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA
    AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG
    GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT
    ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG
    GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC
    AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA
    CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC
    ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02866
    Clone ID Related Accession (Same CDS sequence) NM_001351033.1 , NM_201445.1 , NM_201554.1 , NM_001345.4 , XM_011537992.1 , XM_011537991.1 , XM_011537993.1 , NM_001345.5 , NM_201444.2 , NM_001351034.1 , XM_011537993.2
    Accession Version XM_011537993.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011537993.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA
    CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT
    CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG
    GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG
    AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT
    CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC
    ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG
    TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG
    CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT
    GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC
    TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC
    TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG
    AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT
    GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA
    TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA
    TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG
    ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC
    CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG
    AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG
    TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC
    ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG
    CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA
    TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT
    GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA
    AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG
    GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT
    ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG
    GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC
    AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA
    CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC
    ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011536295.1
    CDS126..2333
    Translation

    Target ORF information:

    RefSeq Version XM_011537993.2
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011537993.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA
    CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT
    CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG
    GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG
    AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT
    CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC
    ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG
    TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG
    CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT
    GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC
    TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC
    TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG
    AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT
    GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA
    TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA
    TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG
    ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC
    CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG
    AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG
    TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC
    ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG
    CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA
    TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT
    GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA
    AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG
    GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT
    ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG
    GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC
    AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA
    CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC
    ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102466
    Clone ID Related Accession (Same CDS sequence) NM_001351038.1 , NM_001351039.1 , NM_001351040.1
    Accession Version NM_001351038.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-30
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025162.48. Transcript Variant: This variant (10), as well as variants 11 and 12, encodes isoform d. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY930112.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153031, SAMEA2156670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGATGATT TAAATTTGAG CACCTCTGAG GCTCTGCGGA TTGACCCTGT TCCTAACACC 
    CACCCACTTC TCGTCTTTGT CAATCCTAAG AGTGGCGGGA AGCAGGGGCA AAGGGTGCTC
    TGGAAGTTCC AGTATATATT AAACCCTCGA CAGGTGTTCA ACCTCCTAAA GGATGGTCCT
    GAGATAGGGC TCCGATTATT CAAGGATGTT CCTGATAGCC GGATTTTGGT GTGTGGTGGA
    GACGGCACAG TAGGCTGGAT TCTAGAGACC ATTGACAAAG CTAACTTGCC AGTTTTGCCT
    CCTGTTGCTG TGTTGCCCCT GGGTACTGGA AATGATCTGG CTCGATGCCT AAGATGGGGA
    GGAGGTTATG AAGGACAGAA TCTGGCAAAG ATCCTCAAGG ATTTAGAGAT GAGTAAAGTG
    GTACATATGG ATCGATGGTC TGTGGAGGTG ATACCTCAAC AAACTGAAGA AAAAAGTGAC
    CCAGTCCCCT TTCAAATCAT CAATAACTAC TTCTCTATTG GCGTGGATGC CTCTATTGCT
    CATCGATTCC ACATCATGCG AGAGAAATAT CCGGAGAAGT TCAACAGCAG AATGAAGAAC
    AAGCTATGGT ACTTCGAATT TGCCACATCT GAATCCATCT TCTCAACATG CAAAAAGCTG
    GAGGAGTCTT TGACAGTTGA GATCTGTGGG AAACCGCTGG ATCTGAGCAA CCTGTCCCTA
    GAAGGCATCG CAGTGCTAAA CATCCCTAGC ATGCATGGTG GCTCCAACCT CTGGGGTGAT
    ACCAGGAGAC CCCATGGGGA TATCTATGGG ATCAACCAGG CCTTAGGTGC TACAGCTAAA
    GTCATCACCG ACCCTGATAT CCTGAAAACC TGTGTACCAG ACCTAAGTGA CAAGAGACTG
    GAAGTGGTTG GGCTGGAGGG TGCAATTGAG ATGGGCCAAA TCTATACCAA GCTCAAGAAT
    GCTGGACGTC GGCTGGCCAA GTGCTCTGAG ATCACCTTCC ACACCACAAA AACCCTTCCC
    ATGCAAATTG ACGGAGAACC CTGGATGCAG ACGCCCTGTA CAATCAAGAT CACCCACAAG
    AACCAGATGC CCATGCTCAT GGGCCCACCC CCCCGCTCCA CCAATTTCTT TGGCTTCTTG
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337967.1
    CDS1208..2353
    Translation

    Target ORF information:

    RefSeq Version NM_001351038.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001351038.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGATGATT TAAATTTGAG CACCTCTGAG GCTCTGCGGA TTGACCCTGT TCCTAACACC 
    CACCCACTTC TCGTCTTTGT CAATCCTAAG AGTGGCGGGA AGCAGGGGCA AAGGGTGCTC
    TGGAAGTTCC AGTATATATT AAACCCTCGA CAGGTGTTCA ACCTCCTAAA GGATGGTCCT
    GAGATAGGGC TCCGATTATT CAAGGATGTT CCTGATAGCC GGATTTTGGT GTGTGGTGGA
    GACGGCACAG TAGGCTGGAT TCTAGAGACC ATTGACAAAG CTAACTTGCC AGTTTTGCCT
    CCTGTTGCTG TGTTGCCCCT GGGTACTGGA AATGATCTGG CTCGATGCCT AAGATGGGGA
    GGAGGTTATG AAGGACAGAA TCTGGCAAAG ATCCTCAAGG ATTTAGAGAT GAGTAAAGTG
    GTACATATGG ATCGATGGTC TGTGGAGGTG ATACCTCAAC AAACTGAAGA AAAAAGTGAC
    CCAGTCCCCT TTCAAATCAT CAATAACTAC TTCTCTATTG GCGTGGATGC CTCTATTGCT
    CATCGATTCC ACATCATGCG AGAGAAATAT CCGGAGAAGT TCAACAGCAG AATGAAGAAC
    AAGCTATGGT ACTTCGAATT TGCCACATCT GAATCCATCT TCTCAACATG CAAAAAGCTG
    GAGGAGTCTT TGACAGTTGA GATCTGTGGG AAACCGCTGG ATCTGAGCAA CCTGTCCCTA
    GAAGGCATCG CAGTGCTAAA CATCCCTAGC ATGCATGGTG GCTCCAACCT CTGGGGTGAT
    ACCAGGAGAC CCCATGGGGA TATCTATGGG ATCAACCAGG CCTTAGGTGC TACAGCTAAA
    GTCATCACCG ACCCTGATAT CCTGAAAACC TGTGTACCAG ACCTAAGTGA CAAGAGACTG
    GAAGTGGTTG GGCTGGAGGG TGCAATTGAG ATGGGCCAAA TCTATACCAA GCTCAAGAAT
    GCTGGACGTC GGCTGGCCAA GTGCTCTGAG ATCACCTTCC ACACCACAAA AACCCTTCCC
    ATGCAAATTG ACGGAGAACC CTGGATGCAG ACGCCCTGTA CAATCAAGAT CACCCACAAG
    AACCAGATGC CCATGCTCAT GGGCCCACCC CCCCGCTCCA CCAATTTCTT TGGCTTCTTG
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102466
    Clone ID Related Accession (Same CDS sequence) NM_001351038.1 , NM_001351039.1 , NM_001351040.1
    Accession Version NM_001351039.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-30
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025162.48. Transcript Variant: This variant (11), as well as variants 10 and 12, encodes isoform d. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.86215.1, SRR1660803.84262.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGATGATT TAAATTTGAG CACCTCTGAG GCTCTGCGGA TTGACCCTGT TCCTAACACC 
    CACCCACTTC TCGTCTTTGT CAATCCTAAG AGTGGCGGGA AGCAGGGGCA AAGGGTGCTC
    TGGAAGTTCC AGTATATATT AAACCCTCGA CAGGTGTTCA ACCTCCTAAA GGATGGTCCT
    GAGATAGGGC TCCGATTATT CAAGGATGTT CCTGATAGCC GGATTTTGGT GTGTGGTGGA
    GACGGCACAG TAGGCTGGAT TCTAGAGACC ATTGACAAAG CTAACTTGCC AGTTTTGCCT
    CCTGTTGCTG TGTTGCCCCT GGGTACTGGA AATGATCTGG CTCGATGCCT AAGATGGGGA
    GGAGGTTATG AAGGACAGAA TCTGGCAAAG ATCCTCAAGG ATTTAGAGAT GAGTAAAGTG
    GTACATATGG ATCGATGGTC TGTGGAGGTG ATACCTCAAC AAACTGAAGA AAAAAGTGAC
    CCAGTCCCCT TTCAAATCAT CAATAACTAC TTCTCTATTG GCGTGGATGC CTCTATTGCT
    CATCGATTCC ACATCATGCG AGAGAAATAT CCGGAGAAGT TCAACAGCAG AATGAAGAAC
    AAGCTATGGT ACTTCGAATT TGCCACATCT GAATCCATCT TCTCAACATG CAAAAAGCTG
    GAGGAGTCTT TGACAGTTGA GATCTGTGGG AAACCGCTGG ATCTGAGCAA CCTGTCCCTA
    GAAGGCATCG CAGTGCTAAA CATCCCTAGC ATGCATGGTG GCTCCAACCT CTGGGGTGAT
    ACCAGGAGAC CCCATGGGGA TATCTATGGG ATCAACCAGG CCTTAGGTGC TACAGCTAAA
    GTCATCACCG ACCCTGATAT CCTGAAAACC TGTGTACCAG ACCTAAGTGA CAAGAGACTG
    GAAGTGGTTG GGCTGGAGGG TGCAATTGAG ATGGGCCAAA TCTATACCAA GCTCAAGAAT
    GCTGGACGTC GGCTGGCCAA GTGCTCTGAG ATCACCTTCC ACACCACAAA AACCCTTCCC
    ATGCAAATTG ACGGAGAACC CTGGATGCAG ACGCCCTGTA CAATCAAGAT CACCCACAAG
    AACCAGATGC CCATGCTCAT GGGCCCACCC CCCCGCTCCA CCAATTTCTT TGGCTTCTTG
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337968.1
    CDS1151..2296
    Translation

    Target ORF information:

    RefSeq Version NM_001351039.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001351039.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGATGATT TAAATTTGAG CACCTCTGAG GCTCTGCGGA TTGACCCTGT TCCTAACACC 
    CACCCACTTC TCGTCTTTGT CAATCCTAAG AGTGGCGGGA AGCAGGGGCA AAGGGTGCTC
    TGGAAGTTCC AGTATATATT AAACCCTCGA CAGGTGTTCA ACCTCCTAAA GGATGGTCCT
    GAGATAGGGC TCCGATTATT CAAGGATGTT CCTGATAGCC GGATTTTGGT GTGTGGTGGA
    GACGGCACAG TAGGCTGGAT TCTAGAGACC ATTGACAAAG CTAACTTGCC AGTTTTGCCT
    CCTGTTGCTG TGTTGCCCCT GGGTACTGGA AATGATCTGG CTCGATGCCT AAGATGGGGA
    GGAGGTTATG AAGGACAGAA TCTGGCAAAG ATCCTCAAGG ATTTAGAGAT GAGTAAAGTG
    GTACATATGG ATCGATGGTC TGTGGAGGTG ATACCTCAAC AAACTGAAGA AAAAAGTGAC
    CCAGTCCCCT TTCAAATCAT CAATAACTAC TTCTCTATTG GCGTGGATGC CTCTATTGCT
    CATCGATTCC ACATCATGCG AGAGAAATAT CCGGAGAAGT TCAACAGCAG AATGAAGAAC
    AAGCTATGGT ACTTCGAATT TGCCACATCT GAATCCATCT TCTCAACATG CAAAAAGCTG
    GAGGAGTCTT TGACAGTTGA GATCTGTGGG AAACCGCTGG ATCTGAGCAA CCTGTCCCTA
    GAAGGCATCG CAGTGCTAAA CATCCCTAGC ATGCATGGTG GCTCCAACCT CTGGGGTGAT
    ACCAGGAGAC CCCATGGGGA TATCTATGGG ATCAACCAGG CCTTAGGTGC TACAGCTAAA
    GTCATCACCG ACCCTGATAT CCTGAAAACC TGTGTACCAG ACCTAAGTGA CAAGAGACTG
    GAAGTGGTTG GGCTGGAGGG TGCAATTGAG ATGGGCCAAA TCTATACCAA GCTCAAGAAT
    GCTGGACGTC GGCTGGCCAA GTGCTCTGAG ATCACCTTCC ACACCACAAA AACCCTTCCC
    ATGCAAATTG ACGGAGAACC CTGGATGCAG ACGCCCTGTA CAATCAAGAT CACCCACAAG
    AACCAGATGC CCATGCTCAT GGGCCCACCC CCCCGCTCCA CCAATTTCTT TGGCTTCTTG
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102466
    Clone ID Related Accession (Same CDS sequence) NM_001351038.1 , NM_001351039.1 , NM_001351040.1
    Accession Version NM_001351040.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-30
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025162.48. Transcript Variant: This variant (12), as well as variants 10 and 11, encodes isoform d. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.273309.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGATGATT TAAATTTGAG CACCTCTGAG GCTCTGCGGA TTGACCCTGT TCCTAACACC 
    CACCCACTTC TCGTCTTTGT CAATCCTAAG AGTGGCGGGA AGCAGGGGCA AAGGGTGCTC
    TGGAAGTTCC AGTATATATT AAACCCTCGA CAGGTGTTCA ACCTCCTAAA GGATGGTCCT
    GAGATAGGGC TCCGATTATT CAAGGATGTT CCTGATAGCC GGATTTTGGT GTGTGGTGGA
    GACGGCACAG TAGGCTGGAT TCTAGAGACC ATTGACAAAG CTAACTTGCC AGTTTTGCCT
    CCTGTTGCTG TGTTGCCCCT GGGTACTGGA AATGATCTGG CTCGATGCCT AAGATGGGGA
    GGAGGTTATG AAGGACAGAA TCTGGCAAAG ATCCTCAAGG ATTTAGAGAT GAGTAAAGTG
    GTACATATGG ATCGATGGTC TGTGGAGGTG ATACCTCAAC AAACTGAAGA AAAAAGTGAC
    CCAGTCCCCT TTCAAATCAT CAATAACTAC TTCTCTATTG GCGTGGATGC CTCTATTGCT
    CATCGATTCC ACATCATGCG AGAGAAATAT CCGGAGAAGT TCAACAGCAG AATGAAGAAC
    AAGCTATGGT ACTTCGAATT TGCCACATCT GAATCCATCT TCTCAACATG CAAAAAGCTG
    GAGGAGTCTT TGACAGTTGA GATCTGTGGG AAACCGCTGG ATCTGAGCAA CCTGTCCCTA
    GAAGGCATCG CAGTGCTAAA CATCCCTAGC ATGCATGGTG GCTCCAACCT CTGGGGTGAT
    ACCAGGAGAC CCCATGGGGA TATCTATGGG ATCAACCAGG CCTTAGGTGC TACAGCTAAA
    GTCATCACCG ACCCTGATAT CCTGAAAACC TGTGTACCAG ACCTAAGTGA CAAGAGACTG
    GAAGTGGTTG GGCTGGAGGG TGCAATTGAG ATGGGCCAAA TCTATACCAA GCTCAAGAAT
    GCTGGACGTC GGCTGGCCAA GTGCTCTGAG ATCACCTTCC ACACCACAAA AACCCTTCCC
    ATGCAAATTG ACGGAGAACC CTGGATGCAG ACGCCCTGTA CAATCAAGAT CACCCACAAG
    AACCAGATGC CCATGCTCAT GGGCCCACCC CCCCGCTCCA CCAATTTCTT TGGCTTCTTG
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337969.1
    CDS1283..2428
    Translation

    Target ORF information:

    RefSeq Version NM_001351040.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001351040.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGATGATT TAAATTTGAG CACCTCTGAG GCTCTGCGGA TTGACCCTGT TCCTAACACC 
    CACCCACTTC TCGTCTTTGT CAATCCTAAG AGTGGCGGGA AGCAGGGGCA AAGGGTGCTC
    TGGAAGTTCC AGTATATATT AAACCCTCGA CAGGTGTTCA ACCTCCTAAA GGATGGTCCT
    GAGATAGGGC TCCGATTATT CAAGGATGTT CCTGATAGCC GGATTTTGGT GTGTGGTGGA
    GACGGCACAG TAGGCTGGAT TCTAGAGACC ATTGACAAAG CTAACTTGCC AGTTTTGCCT
    CCTGTTGCTG TGTTGCCCCT GGGTACTGGA AATGATCTGG CTCGATGCCT AAGATGGGGA
    GGAGGTTATG AAGGACAGAA TCTGGCAAAG ATCCTCAAGG ATTTAGAGAT GAGTAAAGTG
    GTACATATGG ATCGATGGTC TGTGGAGGTG ATACCTCAAC AAACTGAAGA AAAAAGTGAC
    CCAGTCCCCT TTCAAATCAT CAATAACTAC TTCTCTATTG GCGTGGATGC CTCTATTGCT
    CATCGATTCC ACATCATGCG AGAGAAATAT CCGGAGAAGT TCAACAGCAG AATGAAGAAC
    AAGCTATGGT ACTTCGAATT TGCCACATCT GAATCCATCT TCTCAACATG CAAAAAGCTG
    GAGGAGTCTT TGACAGTTGA GATCTGTGGG AAACCGCTGG ATCTGAGCAA CCTGTCCCTA
    GAAGGCATCG CAGTGCTAAA CATCCCTAGC ATGCATGGTG GCTCCAACCT CTGGGGTGAT
    ACCAGGAGAC CCCATGGGGA TATCTATGGG ATCAACCAGG CCTTAGGTGC TACAGCTAAA
    GTCATCACCG ACCCTGATAT CCTGAAAACC TGTGTACCAG ACCTAAGTGA CAAGAGACTG
    GAAGTGGTTG GGCTGGAGGG TGCAATTGAG ATGGGCCAAA TCTATACCAA GCTCAAGAAT
    GCTGGACGTC GGCTGGCCAA GTGCTCTGAG ATCACCTTCC ACACCACAAA AACCCTTCCC
    ATGCAAATTG ACGGAGAACC CTGGATGCAG ACGCCCTGTA CAATCAAGAT CACCCACAAG
    AACCAGATGC CCATGCTCAT GGGCCCACCC CCCCGCTCCA CCAATTTCTT TGGCTTCTTG
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu46356
    Clone ID Related Accession (Same CDS sequence) XM_005268689.2 , XM_011537995.2 , XM_005268690.2
    Accession Version XM_005268689.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005268689.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGTCTTAA GAAACAGAAG AGGATGGGGG GAGAGGTTGA GTCCTCTAGG ACTAAATTTG 
    AAAGAAGTGG ACAAAATTAT CCTACAGATG ATGCGAGTGG CTGAATACCT GGATTGGGAT
    GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG ATGATGAAAG AGATTGACTA TGATGGCAGT
    GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG GCTGGGGCCA CCACCGTGCC ACTGCTAGTG
    CTGCTGGGTC TGGAGATGAC TCTGAAGGAC GACGGACAGC ACATGTGGAG GCCCAAGAGG
    TTCCCCAGAC CAGTCTACTG CAATCTGTGC GAGTCAAGCA TTGGTCTTGG CAAACAGGGA
    CTGAGCTGTA ACCTCTGTAA GTACACTGTT CACGACCAGT GTGCCATGAA AGCCCTGCCT
    TGTGAAGTCA GCACCTATGC CAAGTCTCGG AAGGACATTG GTGTCCAATC ACATGTGTGG
    GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC GACCGCTGTC AGAAAAAGAT CCGGATCTAC
    CACAGTCTGA CCGGGCTGCA TTGTGTATGG TGCCACCTAG AGATCCACGA TGACTGCCTG
    CAAGCGGTGG GCCATGAGTG TGACTGTGGG CTGCTCCGGG ATCACATCCT GCCTCCATCT
    TCCATCTATC CCAGTGTCCT GGCCTCTGGA CCGGATCGTA AAAATAGCAA AACAAGCCAG
    AAGACCATGG ATGATTTAAA TTTGAGCACC TCTGAGGCTC TGCGGATTGA CCCTGTTCCT
    AACACCCACC CACTTCTCGT CTTTGTCAAT CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG
    GTGCTCTGGA AGTTCCAGTA TATATTAAAC CCTCGACAGG TGTTCAACCT CCTAAAGGAT
    GGTCCTGAGA TAGGGCTCCG ATTATTCAAG GATGTTCCTG ATAGCCGGAT TTTGGTGTGT
    GGTGGAGACG GCACAGTAGG CTGGATTCTA GAGACCATTG ACAAAGCTAA CTTGCCAGTT
    TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT ACTGGAAATG ATCTGGCTCG ATGCCTAAGA
    TGGGGAGGAG GTTATGAAGG ACAGAATCTG GCAAAGATCC TCAAGGATTT AGAGATGAGT
    AAAGTGGTAC ATATGGATCG ATGGTCTGTG GAGGTGATAC CTCAACAAAC TGAAGAAAAA
    AGTGACCCAG TCCCCTTTCA AATCATCAAT AACTACTTCT CTATTGGCGT GGATGCCTCT
    ATTGCTCATC GATTCCACAT CATGCGAGAG AAATATCCGG AGAAGTTCAA CAGCAGAATG
    AAGAACAAGC TATGGTACTT CGAATTTGCC ACATCTGAAT CCATCTTCTC AACATGCAAA
    AAGCTGGAGG AGTCTTTGAC AGTTGAGATC TGTGGGAAAC CGCTGGATCT GAGCAACCTG
    TCCCTAGAAG GCATCGCAGT GCTAAACATC CCTAGCATGC ATGGTGGCTC CAACCTCTGG
    GGTGATACCA GGAGACCCCA TGGGGATATC TATGGGATCA ACCAGGCCTT AGGTGCTACA
    GCTAAAGTCA TCACCGACCC TGATATCCTG AAAACCTGTG TACCAGACCT AAGTGACAAG
    AGACTGGAAG TGGTTGGGCT GGAGGGTGCA ATTGAGATGG GCCAAATCTA TACCAAGCTC
    AAGAATGCTG GACGTCGGCT GGCCAAGTGC TCTGAGATCA CCTTCCACAC CACAAAAACC
    CTTCCCATGC AAATTGACGG AGAACCCTGG ATGCAGACGC CCTGTACAAT CAAGATCACC
    CACAAGAACC AGATGCCCAT GCTCATGGGC CCACCCCCCC GCTCCACCAA TTTCTTTGGC
    TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005268746.1
    CDS478..2349
    Translation

    Target ORF information:

    RefSeq Version XM_005268689.2
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005268689.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGTCTTAA GAAACAGAAG AGGATGGGGG GAGAGGTTGA GTCCTCTAGG ACTAAATTTG 
    AAAGAAGTGG ACAAAATTAT CCTACAGATG ATGCGAGTGG CTGAATACCT GGATTGGGAT
    GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG ATGATGAAAG AGATTGACTA TGATGGCAGT
    GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG GCTGGGGCCA CCACCGTGCC ACTGCTAGTG
    CTGCTGGGTC TGGAGATGAC TCTGAAGGAC GACGGACAGC ACATGTGGAG GCCCAAGAGG
    TTCCCCAGAC CAGTCTACTG CAATCTGTGC GAGTCAAGCA TTGGTCTTGG CAAACAGGGA
    CTGAGCTGTA ACCTCTGTAA GTACACTGTT CACGACCAGT GTGCCATGAA AGCCCTGCCT
    TGTGAAGTCA GCACCTATGC CAAGTCTCGG AAGGACATTG GTGTCCAATC ACATGTGTGG
    GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC GACCGCTGTC AGAAAAAGAT CCGGATCTAC
    CACAGTCTGA CCGGGCTGCA TTGTGTATGG TGCCACCTAG AGATCCACGA TGACTGCCTG
    CAAGCGGTGG GCCATGAGTG TGACTGTGGG CTGCTCCGGG ATCACATCCT GCCTCCATCT
    TCCATCTATC CCAGTGTCCT GGCCTCTGGA CCGGATCGTA AAAATAGCAA AACAAGCCAG
    AAGACCATGG ATGATTTAAA TTTGAGCACC TCTGAGGCTC TGCGGATTGA CCCTGTTCCT
    AACACCCACC CACTTCTCGT CTTTGTCAAT CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG
    GTGCTCTGGA AGTTCCAGTA TATATTAAAC CCTCGACAGG TGTTCAACCT CCTAAAGGAT
    GGTCCTGAGA TAGGGCTCCG ATTATTCAAG GATGTTCCTG ATAGCCGGAT TTTGGTGTGT
    GGTGGAGACG GCACAGTAGG CTGGATTCTA GAGACCATTG ACAAAGCTAA CTTGCCAGTT
    TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT ACTGGAAATG ATCTGGCTCG ATGCCTAAGA
    TGGGGAGGAG GTTATGAAGG ACAGAATCTG GCAAAGATCC TCAAGGATTT AGAGATGAGT
    AAAGTGGTAC ATATGGATCG ATGGTCTGTG GAGGTGATAC CTCAACAAAC TGAAGAAAAA
    AGTGACCCAG TCCCCTTTCA AATCATCAAT AACTACTTCT CTATTGGCGT GGATGCCTCT
    ATTGCTCATC GATTCCACAT CATGCGAGAG AAATATCCGG AGAAGTTCAA CAGCAGAATG
    AAGAACAAGC TATGGTACTT CGAATTTGCC ACATCTGAAT CCATCTTCTC AACATGCAAA
    AAGCTGGAGG AGTCTTTGAC AGTTGAGATC TGTGGGAAAC CGCTGGATCT GAGCAACCTG
    TCCCTAGAAG GCATCGCAGT GCTAAACATC CCTAGCATGC ATGGTGGCTC CAACCTCTGG
    GGTGATACCA GGAGACCCCA TGGGGATATC TATGGGATCA ACCAGGCCTT AGGTGCTACA
    GCTAAAGTCA TCACCGACCC TGATATCCTG AAAACCTGTG TACCAGACCT AAGTGACAAG
    AGACTGGAAG TGGTTGGGCT GGAGGGTGCA ATTGAGATGG GCCAAATCTA TACCAAGCTC
    AAGAATGCTG GACGTCGGCT GGCCAAGTGC TCTGAGATCA CCTTCCACAC CACAAAAACC
    CTTCCCATGC AAATTGACGG AGAACCCTGG ATGCAGACGC CCTGTACAAT CAAGATCACC
    CACAAGAACC AGATGCCCAT GCTCATGGGC CCACCCCCCC GCTCCACCAA TTTCTTTGGC
    TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu46356
    Clone ID Related Accession (Same CDS sequence) XM_005268689.2 , XM_011537995.2 , XM_005268690.2
    Accession Version XM_011537995.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011537995.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGTCTTAA GAAACAGAAG AGGATGGGGG GAGAGGTTGA GTCCTCTAGG ACTAAATTTG 
    AAAGAAGTGG ACAAAATTAT CCTACAGATG ATGCGAGTGG CTGAATACCT GGATTGGGAT
    GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG ATGATGAAAG AGATTGACTA TGATGGCAGT
    GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG GCTGGGGCCA CCACCGTGCC ACTGCTAGTG
    CTGCTGGGTC TGGAGATGAC TCTGAAGGAC GACGGACAGC ACATGTGGAG GCCCAAGAGG
    TTCCCCAGAC CAGTCTACTG CAATCTGTGC GAGTCAAGCA TTGGTCTTGG CAAACAGGGA
    CTGAGCTGTA ACCTCTGTAA GTACACTGTT CACGACCAGT GTGCCATGAA AGCCCTGCCT
    TGTGAAGTCA GCACCTATGC CAAGTCTCGG AAGGACATTG GTGTCCAATC ACATGTGTGG
    GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC GACCGCTGTC AGAAAAAGAT CCGGATCTAC
    CACAGTCTGA CCGGGCTGCA TTGTGTATGG TGCCACCTAG AGATCCACGA TGACTGCCTG
    CAAGCGGTGG GCCATGAGTG TGACTGTGGG CTGCTCCGGG ATCACATCCT GCCTCCATCT
    TCCATCTATC CCAGTGTCCT GGCCTCTGGA CCGGATCGTA AAAATAGCAA AACAAGCCAG
    AAGACCATGG ATGATTTAAA TTTGAGCACC TCTGAGGCTC TGCGGATTGA CCCTGTTCCT
    AACACCCACC CACTTCTCGT CTTTGTCAAT CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG
    GTGCTCTGGA AGTTCCAGTA TATATTAAAC CCTCGACAGG TGTTCAACCT CCTAAAGGAT
    GGTCCTGAGA TAGGGCTCCG ATTATTCAAG GATGTTCCTG ATAGCCGGAT TTTGGTGTGT
    GGTGGAGACG GCACAGTAGG CTGGATTCTA GAGACCATTG ACAAAGCTAA CTTGCCAGTT
    TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT ACTGGAAATG ATCTGGCTCG ATGCCTAAGA
    TGGGGAGGAG GTTATGAAGG ACAGAATCTG GCAAAGATCC TCAAGGATTT AGAGATGAGT
    AAAGTGGTAC ATATGGATCG ATGGTCTGTG GAGGTGATAC CTCAACAAAC TGAAGAAAAA
    AGTGACCCAG TCCCCTTTCA AATCATCAAT AACTACTTCT CTATTGGCGT GGATGCCTCT
    ATTGCTCATC GATTCCACAT CATGCGAGAG AAATATCCGG AGAAGTTCAA CAGCAGAATG
    AAGAACAAGC TATGGTACTT CGAATTTGCC ACATCTGAAT CCATCTTCTC AACATGCAAA
    AAGCTGGAGG AGTCTTTGAC AGTTGAGATC TGTGGGAAAC CGCTGGATCT GAGCAACCTG
    TCCCTAGAAG GCATCGCAGT GCTAAACATC CCTAGCATGC ATGGTGGCTC CAACCTCTGG
    GGTGATACCA GGAGACCCCA TGGGGATATC TATGGGATCA ACCAGGCCTT AGGTGCTACA
    GCTAAAGTCA TCACCGACCC TGATATCCTG AAAACCTGTG TACCAGACCT AAGTGACAAG
    AGACTGGAAG TGGTTGGGCT GGAGGGTGCA ATTGAGATGG GCCAAATCTA TACCAAGCTC
    AAGAATGCTG GACGTCGGCT GGCCAAGTGC TCTGAGATCA CCTTCCACAC CACAAAAACC
    CTTCCCATGC AAATTGACGG AGAACCCTGG ATGCAGACGC CCTGTACAAT CAAGATCACC
    CACAAGAACC AGATGCCCAT GCTCATGGGC CCACCCCCCC GCTCCACCAA TTTCTTTGGC
    TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011536297.1
    CDS473..2344
    Translation

    Target ORF information:

    RefSeq Version XM_011537995.2
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011537995.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGTCTTAA GAAACAGAAG AGGATGGGGG GAGAGGTTGA GTCCTCTAGG ACTAAATTTG 
    AAAGAAGTGG ACAAAATTAT CCTACAGATG ATGCGAGTGG CTGAATACCT GGATTGGGAT
    GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG ATGATGAAAG AGATTGACTA TGATGGCAGT
    GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG GCTGGGGCCA CCACCGTGCC ACTGCTAGTG
    CTGCTGGGTC TGGAGATGAC TCTGAAGGAC GACGGACAGC ACATGTGGAG GCCCAAGAGG
    TTCCCCAGAC CAGTCTACTG CAATCTGTGC GAGTCAAGCA TTGGTCTTGG CAAACAGGGA
    CTGAGCTGTA ACCTCTGTAA GTACACTGTT CACGACCAGT GTGCCATGAA AGCCCTGCCT
    TGTGAAGTCA GCACCTATGC CAAGTCTCGG AAGGACATTG GTGTCCAATC ACATGTGTGG
    GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC GACCGCTGTC AGAAAAAGAT CCGGATCTAC
    CACAGTCTGA CCGGGCTGCA TTGTGTATGG TGCCACCTAG AGATCCACGA TGACTGCCTG
    CAAGCGGTGG GCCATGAGTG TGACTGTGGG CTGCTCCGGG ATCACATCCT GCCTCCATCT
    TCCATCTATC CCAGTGTCCT GGCCTCTGGA CCGGATCGTA AAAATAGCAA AACAAGCCAG
    AAGACCATGG ATGATTTAAA TTTGAGCACC TCTGAGGCTC TGCGGATTGA CCCTGTTCCT
    AACACCCACC CACTTCTCGT CTTTGTCAAT CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG
    GTGCTCTGGA AGTTCCAGTA TATATTAAAC CCTCGACAGG TGTTCAACCT CCTAAAGGAT
    GGTCCTGAGA TAGGGCTCCG ATTATTCAAG GATGTTCCTG ATAGCCGGAT TTTGGTGTGT
    GGTGGAGACG GCACAGTAGG CTGGATTCTA GAGACCATTG ACAAAGCTAA CTTGCCAGTT
    TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT ACTGGAAATG ATCTGGCTCG ATGCCTAAGA
    TGGGGAGGAG GTTATGAAGG ACAGAATCTG GCAAAGATCC TCAAGGATTT AGAGATGAGT
    AAAGTGGTAC ATATGGATCG ATGGTCTGTG GAGGTGATAC CTCAACAAAC TGAAGAAAAA
    AGTGACCCAG TCCCCTTTCA AATCATCAAT AACTACTTCT CTATTGGCGT GGATGCCTCT
    ATTGCTCATC GATTCCACAT CATGCGAGAG AAATATCCGG AGAAGTTCAA CAGCAGAATG
    AAGAACAAGC TATGGTACTT CGAATTTGCC ACATCTGAAT CCATCTTCTC AACATGCAAA
    AAGCTGGAGG AGTCTTTGAC AGTTGAGATC TGTGGGAAAC CGCTGGATCT GAGCAACCTG
    TCCCTAGAAG GCATCGCAGT GCTAAACATC CCTAGCATGC ATGGTGGCTC CAACCTCTGG
    GGTGATACCA GGAGACCCCA TGGGGATATC TATGGGATCA ACCAGGCCTT AGGTGCTACA
    GCTAAAGTCA TCACCGACCC TGATATCCTG AAAACCTGTG TACCAGACCT AAGTGACAAG
    AGACTGGAAG TGGTTGGGCT GGAGGGTGCA ATTGAGATGG GCCAAATCTA TACCAAGCTC
    AAGAATGCTG GACGTCGGCT GGCCAAGTGC TCTGAGATCA CCTTCCACAC CACAAAAACC
    CTTCCCATGC AAATTGACGG AGAACCCTGG ATGCAGACGC CCTGTACAAT CAAGATCACC
    CACAAGAACC AGATGCCCAT GCTCATGGGC CCACCCCCCC GCTCCACCAA TTTCTTTGGC
    TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu46356
    Clone ID Related Accession (Same CDS sequence) XM_005268689.2 , XM_011537995.2 , XM_005268690.2
    Accession Version XM_005268690.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005268690.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGTCTTAA GAAACAGAAG AGGATGGGGG GAGAGGTTGA GTCCTCTAGG ACTAAATTTG 
    AAAGAAGTGG ACAAAATTAT CCTACAGATG ATGCGAGTGG CTGAATACCT GGATTGGGAT
    GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG ATGATGAAAG AGATTGACTA TGATGGCAGT
    GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG GCTGGGGCCA CCACCGTGCC ACTGCTAGTG
    CTGCTGGGTC TGGAGATGAC TCTGAAGGAC GACGGACAGC ACATGTGGAG GCCCAAGAGG
    TTCCCCAGAC CAGTCTACTG CAATCTGTGC GAGTCAAGCA TTGGTCTTGG CAAACAGGGA
    CTGAGCTGTA ACCTCTGTAA GTACACTGTT CACGACCAGT GTGCCATGAA AGCCCTGCCT
    TGTGAAGTCA GCACCTATGC CAAGTCTCGG AAGGACATTG GTGTCCAATC ACATGTGTGG
    GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC GACCGCTGTC AGAAAAAGAT CCGGATCTAC
    CACAGTCTGA CCGGGCTGCA TTGTGTATGG TGCCACCTAG AGATCCACGA TGACTGCCTG
    CAAGCGGTGG GCCATGAGTG TGACTGTGGG CTGCTCCGGG ATCACATCCT GCCTCCATCT
    TCCATCTATC CCAGTGTCCT GGCCTCTGGA CCGGATCGTA AAAATAGCAA AACAAGCCAG
    AAGACCATGG ATGATTTAAA TTTGAGCACC TCTGAGGCTC TGCGGATTGA CCCTGTTCCT
    AACACCCACC CACTTCTCGT CTTTGTCAAT CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG
    GTGCTCTGGA AGTTCCAGTA TATATTAAAC CCTCGACAGG TGTTCAACCT CCTAAAGGAT
    GGTCCTGAGA TAGGGCTCCG ATTATTCAAG GATGTTCCTG ATAGCCGGAT TTTGGTGTGT
    GGTGGAGACG GCACAGTAGG CTGGATTCTA GAGACCATTG ACAAAGCTAA CTTGCCAGTT
    TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT ACTGGAAATG ATCTGGCTCG ATGCCTAAGA
    TGGGGAGGAG GTTATGAAGG ACAGAATCTG GCAAAGATCC TCAAGGATTT AGAGATGAGT
    AAAGTGGTAC ATATGGATCG ATGGTCTGTG GAGGTGATAC CTCAACAAAC TGAAGAAAAA
    AGTGACCCAG TCCCCTTTCA AATCATCAAT AACTACTTCT CTATTGGCGT GGATGCCTCT
    ATTGCTCATC GATTCCACAT CATGCGAGAG AAATATCCGG AGAAGTTCAA CAGCAGAATG
    AAGAACAAGC TATGGTACTT CGAATTTGCC ACATCTGAAT CCATCTTCTC AACATGCAAA
    AAGCTGGAGG AGTCTTTGAC AGTTGAGATC TGTGGGAAAC CGCTGGATCT GAGCAACCTG
    TCCCTAGAAG GCATCGCAGT GCTAAACATC CCTAGCATGC ATGGTGGCTC CAACCTCTGG
    GGTGATACCA GGAGACCCCA TGGGGATATC TATGGGATCA ACCAGGCCTT AGGTGCTACA
    GCTAAAGTCA TCACCGACCC TGATATCCTG AAAACCTGTG TACCAGACCT AAGTGACAAG
    AGACTGGAAG TGGTTGGGCT GGAGGGTGCA ATTGAGATGG GCCAAATCTA TACCAAGCTC
    AAGAATGCTG GACGTCGGCT GGCCAAGTGC TCTGAGATCA CCTTCCACAC CACAAAAACC
    CTTCCCATGC AAATTGACGG AGAACCCTGG ATGCAGACGC CCTGTACAAT CAAGATCACC
    CACAAGAACC AGATGCCCAT GCTCATGGGC CCACCCCCCC GCTCCACCAA TTTCTTTGGC
    TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005268747.1
    CDS298..2169
    Translation

    Target ORF information:

    RefSeq Version XM_005268690.2
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005268690.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGTCTTAA GAAACAGAAG AGGATGGGGG GAGAGGTTGA GTCCTCTAGG ACTAAATTTG 
    AAAGAAGTGG ACAAAATTAT CCTACAGATG ATGCGAGTGG CTGAATACCT GGATTGGGAT
    GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG ATGATGAAAG AGATTGACTA TGATGGCAGT
    GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG GCTGGGGCCA CCACCGTGCC ACTGCTAGTG
    CTGCTGGGTC TGGAGATGAC TCTGAAGGAC GACGGACAGC ACATGTGGAG GCCCAAGAGG
    TTCCCCAGAC CAGTCTACTG CAATCTGTGC GAGTCAAGCA TTGGTCTTGG CAAACAGGGA
    CTGAGCTGTA ACCTCTGTAA GTACACTGTT CACGACCAGT GTGCCATGAA AGCCCTGCCT
    TGTGAAGTCA GCACCTATGC CAAGTCTCGG AAGGACATTG GTGTCCAATC ACATGTGTGG
    GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC GACCGCTGTC AGAAAAAGAT CCGGATCTAC
    CACAGTCTGA CCGGGCTGCA TTGTGTATGG TGCCACCTAG AGATCCACGA TGACTGCCTG
    CAAGCGGTGG GCCATGAGTG TGACTGTGGG CTGCTCCGGG ATCACATCCT GCCTCCATCT
    TCCATCTATC CCAGTGTCCT GGCCTCTGGA CCGGATCGTA AAAATAGCAA AACAAGCCAG
    AAGACCATGG ATGATTTAAA TTTGAGCACC TCTGAGGCTC TGCGGATTGA CCCTGTTCCT
    AACACCCACC CACTTCTCGT CTTTGTCAAT CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG
    GTGCTCTGGA AGTTCCAGTA TATATTAAAC CCTCGACAGG TGTTCAACCT CCTAAAGGAT
    GGTCCTGAGA TAGGGCTCCG ATTATTCAAG GATGTTCCTG ATAGCCGGAT TTTGGTGTGT
    GGTGGAGACG GCACAGTAGG CTGGATTCTA GAGACCATTG ACAAAGCTAA CTTGCCAGTT
    TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT ACTGGAAATG ATCTGGCTCG ATGCCTAAGA
    TGGGGAGGAG GTTATGAAGG ACAGAATCTG GCAAAGATCC TCAAGGATTT AGAGATGAGT
    AAAGTGGTAC ATATGGATCG ATGGTCTGTG GAGGTGATAC CTCAACAAAC TGAAGAAAAA
    AGTGACCCAG TCCCCTTTCA AATCATCAAT AACTACTTCT CTATTGGCGT GGATGCCTCT
    ATTGCTCATC GATTCCACAT CATGCGAGAG AAATATCCGG AGAAGTTCAA CAGCAGAATG
    AAGAACAAGC TATGGTACTT CGAATTTGCC ACATCTGAAT CCATCTTCTC AACATGCAAA
    AAGCTGGAGG AGTCTTTGAC AGTTGAGATC TGTGGGAAAC CGCTGGATCT GAGCAACCTG
    TCCCTAGAAG GCATCGCAGT GCTAAACATC CCTAGCATGC ATGGTGGCTC CAACCTCTGG
    GGTGATACCA GGAGACCCCA TGGGGATATC TATGGGATCA ACCAGGCCTT AGGTGCTACA
    GCTAAAGTCA TCACCGACCC TGATATCCTG AAAACCTGTG TACCAGACCT AAGTGACAAG
    AGACTGGAAG TGGTTGGGCT GGAGGGTGCA ATTGAGATGG GCCAAATCTA TACCAAGCTC
    AAGAATGCTG GACGTCGGCT GGCCAAGTGC TCTGAGATCA CCTTCCACAC CACAAAAACC
    CTTCCCATGC AAATTGACGG AGAACCCTGG ATGCAGACGC CCTGTACAAT CAAGATCACC
    CACAAGAACC AGATGCCCAT GCTCATGGGC CCACCCCCCC GCTCCACCAA TTTCTTTGGC
    TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102467
    Clone ID Related Accession (Same CDS sequence) NM_001351036.1 , NM_001351037.1
    Accession Version NM_001351036.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-30
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025162.48. Transcript Variant: This variant (8), as well as variant 9, encodes isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.249828.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGCGAG TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAG 
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TCTCTCAAGC TGAGTGGGTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG
    GACGACGGAC AGCACATGTG GAGGCCCAAG AGGTTCCCCA GACCAGTCTA CTGCAATCTG
    TGCGAGTCAA GCATTGGTCT TGGCAAACAG GGACTGAGCT GTAACCTCTG TAAGTACACT
    GTTCACGACC AGTGTGCCAT GAAAGCCCTG CCTTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA GTCCGGGCGC
    TGCGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGACCGGGCT GCATTGTGTA
    TGGTGCCACC TAGAGATCCA CGATGACTGC CTGCAAGCGG TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GGGATCACAT CCTGCCTCCA TCTTCCATCT ATCCCAGTGT CCTGGCCTCT
    GGACCGGATC GTAAAAATAG CAAAACAAGC CAGAAGACCA TGGATGATTT AAATTTGAGC
    ACCTCTGAGG CTCTGCGGAT TGACCCTGTT CCTAACACCC ACCCACTTCT CGTCTTTGTC
    AATCCTAAGA GTGGCGGGAA GCAGGGGCAA AGGGTGCTCT GGAAGTTCCA GTATATATTA
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGATAGGGCT CCGATTATTC
    AAGGATGTTC CTGATAGCCG GATTTTGGTG TGTGGTGGAG ACGGCACAGT AGGCTGGATT
    CTAGAGACCA TTGACAAAGC TAACTTGCCA GTTTTGCCTC CTGTTGCTGT GTTGCCCCTG
    GGTACTGGAA ATGATCTGGC TCGATGCCTA AGATGGGGAG GAGGTTATGA AGGACAGAAT
    CTGGCAAAGA TCCTCAAGGA TTTAGAGATG AGTAAAGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCTCAACA AACTGAAGAA AAAAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCTATTGG CGTGGATGCC TCTATTGCTC ATCGATTCCA CATCATGCGA
    GAGAAATATC CGGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACATCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAGTCTTT GACAGTTGAG
    ATCTGTGGGA AACCGCTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATCGC AGTGCTAAAC
    ATCCCTAGCA TGCATGGTGG CTCCAACCTC TGGGGTGATA CCAGGAGACC CCATGGGGAT
    ATCTATGGGA TCAACCAGGC CTTAGGTGCT ACAGCTAAAG TCATCACCGA CCCTGATATC
    CTGAAAACCT GTGTACCAGA CCTAAGTGAC AAGAGACTGG AAGTGGTTGG GCTGGAGGGT
    GCAATTGAGA TGGGCCAAAT CTATACCAAG CTCAAGAATG CTGGACGTCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTTCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CGCCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTCATG
    GGCCCACCCC CCCGCTCCAC CAATTTCTTT GGCTTCTTGA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337965.1
    CDS615..2399
    Translation

    Target ORF information:

    RefSeq Version NM_001351036.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001351036.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGCGAG TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAG 
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TCTCTCAAGC TGAGTGGGTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG
    GACGACGGAC AGCACATGTG GAGGCCCAAG AGGTTCCCCA GACCAGTCTA CTGCAATCTG
    TGCGAGTCAA GCATTGGTCT TGGCAAACAG GGACTGAGCT GTAACCTCTG TAAGTACACT
    GTTCACGACC AGTGTGCCAT GAAAGCCCTG CCTTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA GTCCGGGCGC
    TGCGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGACCGGGCT GCATTGTGTA
    TGGTGCCACC TAGAGATCCA CGATGACTGC CTGCAAGCGG TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GGGATCACAT CCTGCCTCCA TCTTCCATCT ATCCCAGTGT CCTGGCCTCT
    GGACCGGATC GTAAAAATAG CAAAACAAGC CAGAAGACCA TGGATGATTT AAATTTGAGC
    ACCTCTGAGG CTCTGCGGAT TGACCCTGTT CCTAACACCC ACCCACTTCT CGTCTTTGTC
    AATCCTAAGA GTGGCGGGAA GCAGGGGCAA AGGGTGCTCT GGAAGTTCCA GTATATATTA
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGATAGGGCT CCGATTATTC
    AAGGATGTTC CTGATAGCCG GATTTTGGTG TGTGGTGGAG ACGGCACAGT AGGCTGGATT
    CTAGAGACCA TTGACAAAGC TAACTTGCCA GTTTTGCCTC CTGTTGCTGT GTTGCCCCTG
    GGTACTGGAA ATGATCTGGC TCGATGCCTA AGATGGGGAG GAGGTTATGA AGGACAGAAT
    CTGGCAAAGA TCCTCAAGGA TTTAGAGATG AGTAAAGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCTCAACA AACTGAAGAA AAAAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCTATTGG CGTGGATGCC TCTATTGCTC ATCGATTCCA CATCATGCGA
    GAGAAATATC CGGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACATCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAGTCTTT GACAGTTGAG
    ATCTGTGGGA AACCGCTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATCGC AGTGCTAAAC
    ATCCCTAGCA TGCATGGTGG CTCCAACCTC TGGGGTGATA CCAGGAGACC CCATGGGGAT
    ATCTATGGGA TCAACCAGGC CTTAGGTGCT ACAGCTAAAG TCATCACCGA CCCTGATATC
    CTGAAAACCT GTGTACCAGA CCTAAGTGAC AAGAGACTGG AAGTGGTTGG GCTGGAGGGT
    GCAATTGAGA TGGGCCAAAT CTATACCAAG CTCAAGAATG CTGGACGTCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTTCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CGCCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTCATG
    GGCCCACCCC CCCGCTCCAC CAATTTCTTT GGCTTCTTGA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102467
    Clone ID Related Accession (Same CDS sequence) NM_001351036.1 , NM_001351037.1
    Accession Version NM_001351037.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-30
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025162.48. On Apr 29, 2017 this sequence version replaced XM_017018909.1. Transcript Variant: This variant (9), as well as variant 8, encodes isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.168435.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGCGAG TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAG 
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TCTCTCAAGC TGAGTGGGTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG
    GACGACGGAC AGCACATGTG GAGGCCCAAG AGGTTCCCCA GACCAGTCTA CTGCAATCTG
    TGCGAGTCAA GCATTGGTCT TGGCAAACAG GGACTGAGCT GTAACCTCTG TAAGTACACT
    GTTCACGACC AGTGTGCCAT GAAAGCCCTG CCTTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA GTCCGGGCGC
    TGCGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGACCGGGCT GCATTGTGTA
    TGGTGCCACC TAGAGATCCA CGATGACTGC CTGCAAGCGG TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GGGATCACAT CCTGCCTCCA TCTTCCATCT ATCCCAGTGT CCTGGCCTCT
    GGACCGGATC GTAAAAATAG CAAAACAAGC CAGAAGACCA TGGATGATTT AAATTTGAGC
    ACCTCTGAGG CTCTGCGGAT TGACCCTGTT CCTAACACCC ACCCACTTCT CGTCTTTGTC
    AATCCTAAGA GTGGCGGGAA GCAGGGGCAA AGGGTGCTCT GGAAGTTCCA GTATATATTA
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGATAGGGCT CCGATTATTC
    AAGGATGTTC CTGATAGCCG GATTTTGGTG TGTGGTGGAG ACGGCACAGT AGGCTGGATT
    CTAGAGACCA TTGACAAAGC TAACTTGCCA GTTTTGCCTC CTGTTGCTGT GTTGCCCCTG
    GGTACTGGAA ATGATCTGGC TCGATGCCTA AGATGGGGAG GAGGTTATGA AGGACAGAAT
    CTGGCAAAGA TCCTCAAGGA TTTAGAGATG AGTAAAGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCTCAACA AACTGAAGAA AAAAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCTATTGG CGTGGATGCC TCTATTGCTC ATCGATTCCA CATCATGCGA
    GAGAAATATC CGGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACATCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAGTCTTT GACAGTTGAG
    ATCTGTGGGA AACCGCTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATCGC AGTGCTAAAC
    ATCCCTAGCA TGCATGGTGG CTCCAACCTC TGGGGTGATA CCAGGAGACC CCATGGGGAT
    ATCTATGGGA TCAACCAGGC CTTAGGTGCT ACAGCTAAAG TCATCACCGA CCCTGATATC
    CTGAAAACCT GTGTACCAGA CCTAAGTGAC AAGAGACTGG AAGTGGTTGG GCTGGAGGGT
    GCAATTGAGA TGGGCCAAAT CTATACCAAG CTCAAGAATG CTGGACGTCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTTCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CGCCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTCATG
    GGCCCACCCC CCCGCTCCAC CAATTTCTTT GGCTTCTTGA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337966.1
    CDS647..2431
    Translation

    Target ORF information:

    RefSeq Version NM_001351037.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001351037.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGCGAG TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAG 
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TCTCTCAAGC TGAGTGGGTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG
    GACGACGGAC AGCACATGTG GAGGCCCAAG AGGTTCCCCA GACCAGTCTA CTGCAATCTG
    TGCGAGTCAA GCATTGGTCT TGGCAAACAG GGACTGAGCT GTAACCTCTG TAAGTACACT
    GTTCACGACC AGTGTGCCAT GAAAGCCCTG CCTTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA GTCCGGGCGC
    TGCGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGACCGGGCT GCATTGTGTA
    TGGTGCCACC TAGAGATCCA CGATGACTGC CTGCAAGCGG TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GGGATCACAT CCTGCCTCCA TCTTCCATCT ATCCCAGTGT CCTGGCCTCT
    GGACCGGATC GTAAAAATAG CAAAACAAGC CAGAAGACCA TGGATGATTT AAATTTGAGC
    ACCTCTGAGG CTCTGCGGAT TGACCCTGTT CCTAACACCC ACCCACTTCT CGTCTTTGTC
    AATCCTAAGA GTGGCGGGAA GCAGGGGCAA AGGGTGCTCT GGAAGTTCCA GTATATATTA
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGATAGGGCT CCGATTATTC
    AAGGATGTTC CTGATAGCCG GATTTTGGTG TGTGGTGGAG ACGGCACAGT AGGCTGGATT
    CTAGAGACCA TTGACAAAGC TAACTTGCCA GTTTTGCCTC CTGTTGCTGT GTTGCCCCTG
    GGTACTGGAA ATGATCTGGC TCGATGCCTA AGATGGGGAG GAGGTTATGA AGGACAGAAT
    CTGGCAAAGA TCCTCAAGGA TTTAGAGATG AGTAAAGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCTCAACA AACTGAAGAA AAAAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCTATTGG CGTGGATGCC TCTATTGCTC ATCGATTCCA CATCATGCGA
    GAGAAATATC CGGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACATCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAGTCTTT GACAGTTGAG
    ATCTGTGGGA AACCGCTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATCGC AGTGCTAAAC
    ATCCCTAGCA TGCATGGTGG CTCCAACCTC TGGGGTGATA CCAGGAGACC CCATGGGGAT
    ATCTATGGGA TCAACCAGGC CTTAGGTGCT ACAGCTAAAG TCATCACCGA CCCTGATATC
    CTGAAAACCT GTGTACCAGA CCTAAGTGAC AAGAGACTGG AAGTGGTTGG GCTGGAGGGT
    GCAATTGAGA TGGGCCAAAT CTATACCAAG CTCAAGAATG CTGGACGTCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTTCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CGCCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTCATG
    GGCCCACCCC CCCGCTCCAC CAATTTCTTT GGCTTCTTGA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu46355
    Clone ID Related Accession (Same CDS sequence) NM_001351035.1
    Accession Version NM_001351035.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2322bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-30
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025162.48. On Apr 29, 2017 this sequence version replaced XM_005268688.2. Transcript Variant: This variant (7) encodes the longest isoform (b). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.49634.1, SRR1803611.243910.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGGTCAT GGCCAGGAAG ACGCGCTAAC GCAGCCTACC CTCTGAAGAG GTCCAAGCAA 
    CGGAAGTACT ACTACGAAGC TGCCTTTCTG GCCATCCTTG AGAAAAATAG ACAGATGGCC
    AAGGAGAGGG GCCTAATAAG CCCCAGTGAT TTTGCCCAGC TGCAAAAATA CATGGAATAC
    TCCACCAAAA AGGTCAGTGA TGTCCTAAAG CTCTTCGAGG ATGGCGAGAT GGCTAAATAT
    GTCCAAGGAG ATGCCATTGG GTACGAGGGA TTCCAGCAAT TCCTGAAAAT CTATCTCGAA
    GTGGATAATG TTCCCAGACA CCTAAGCCTG GCACTGTTTC AATCCTTTGA GACTGGTCAC
    TGCTTAAATG AGACAAATGT GACAAAAGAT GTGGTGTGTC TCAATGATGT TTCCTGCTAC
    TTTTCCCTTC TGGAGGGTGG TCGGCCAGAA GACAAGTTAG AATTCACCTT CAAGCTGTAC
    GACACGGACA GAAATGGGAT CCTGGACAGC TCAGAAGTGG ACAAAATTAT CCTACAGATG
    ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG
    ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG
    GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC
    GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC
    GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT
    CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG
    AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC
    GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG
    TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG
    CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA
    CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC
    TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT
    CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC
    CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG
    GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA
    GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT
    ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG
    GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG
    GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT
    AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG
    AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC
    ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC
    TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC
    CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC
    TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG
    AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA
    ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC
    TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG
    ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC
    CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337964.1
    CDS93..2414
    Translation

    Target ORF information:

    RefSeq Version NM_001351035.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001351035.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGGTCAT GGCCAGGAAG ACGCGCTAAC GCAGCCTACC CTCTGAAGAG GTCCAAGCAA 
    CGGAAGTACT ACTACGAAGC TGCCTTTCTG GCCATCCTTG AGAAAAATAG ACAGATGGCC
    AAGGAGAGGG GCCTAATAAG CCCCAGTGAT TTTGCCCAGC TGCAAAAATA CATGGAATAC
    TCCACCAAAA AGGTCAGTGA TGTCCTAAAG CTCTTCGAGG ATGGCGAGAT GGCTAAATAT
    GTCCAAGGAG ATGCCATTGG GTACGAGGGA TTCCAGCAAT TCCTGAAAAT CTATCTCGAA
    GTGGATAATG TTCCCAGACA CCTAAGCCTG GCACTGTTTC AATCCTTTGA GACTGGTCAC
    TGCTTAAATG AGACAAATGT GACAAAAGAT GTGGTGTGTC TCAATGATGT TTCCTGCTAC
    TTTTCCCTTC TGGAGGGTGG TCGGCCAGAA GACAAGTTAG AATTCACCTT CAAGCTGTAC
    GACACGGACA GAAATGGGAT CCTGGACAGC TCAGAAGTGG ACAAAATTAT CCTACAGATG
    ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG
    ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG
    GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC
    GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC
    GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT
    CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG
    AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC
    GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG
    TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG
    CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA
    CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC
    TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT
    CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC
    CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG
    GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA
    GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT
    ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG
    GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG
    GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT
    AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG
    AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC
    ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC
    TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC
    CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC
    TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG
    AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA
    ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC
    TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG
    ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC
    CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu83567
    Clone ID Related Accession (Same CDS sequence) XM_017018900.1
    Accession Version XM_017018900.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2676bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGGGTCAT GGCCAGGAAG ACGCGCTAAC GCAGCCTACC CTCTGAAGAG GTCCAAGCAA 
    CGGAAGTACT ACTACGAAGC TGCCTTTCTG GCCATCCTTG AGAAAAATAG ACAGATGGCC
    AAGGAGAGGG GCCTAATAAG CCCCAGTGAT TTTGCCCAGC TGCAAAAATA CATGGAATAC
    TCCACCAAAA AGGTCAGTGA TGTCCTAAAG CTCTTCGAGG ATGGCGAGAT GGCTAAATAT
    GTCCAAGGAG ATGCCATTGG GTACGAGGGA TTCCAGCAAT TCCTGAAAAT CTATCTCGAA
    GTGGATAATG TTCCCAGACA CCTAAGCCTG GCACTGTTTC AATCCTTTGA GACTGGTCAC
    TGCTTAAATG AGACAAATGT GACAAAAGAT GTGGTGTGTC TCAATGATGT TTCCTGCTAC
    TTTTCCCTTC TGGAGGGTGG TCGGCCAGAA GACAAGTTAG AATTCACCTT CAAGCTGTAC
    GACACGGACA GAAATGGGAT CCTGGACAGC TCAGTGAGTT GGGGACCCTG TATGCTGGGC
    AAGGGAATAT GTGTTAATTT GTCTGCACCC TCCTGCCCCA ACTCTTCCAG GGTCTTAAGA
    AACAGAAGAG GATGGGGGGA GAGGTTGAGT CCTCTAGGAC TAAATTTGAA AGTAAGTCCC
    AAGCTCTTGA CCCCTCAAAG AGAATGCTGG ATACATGAAT TGGGTCTGCC TTACAAACAT
    ACAAACACAT TTATTTACTT GGCAGACATA CCTGAGAATG AGAGGTGTGA ATCTGGGGTG
    GAACCCAGGA ACACTGATTT CTGGAAAAGA GTTTGGATGG TGGTAGTAAA GGGGATATGG
    CTGTGGCTCT TGCCCTTTTT GCTCCAGGAA GTGGACAAAA TTATCCTACA GATGATGCGA
    GTGGCTGAAT ACCTGGATTG GGATGTGTCT GAGCTGAGGC CGATTCTTCA GGAGATGATG
    AAAGAGATTG ACTATGATGG CAGTGGCTCT GTCTCTCAAG CTGAGTGGGT CCGGGCTGGG
    GCCACCACCG TGCCACTGCT AGTGCTGCTG GGTCTGGAGA TGACTCTGAA GGACGACGGA
    CAGCACATGT GGAGGCCCAA GAGGTTCCCC AGACCAGTCT ACTGCAATCT GTGCGAGTCA
    AGCATTGGTC TTGGCAAACA GGGACTGAGC TGTAACCTCT GTAAGTACAC TGTTCACGAC
    CAGTGTGCCA TGAAAGCCCT GCCTTGTGAA GTCAGCACCT ATGCCAAGTC TCGGAAGGAC
    ATTGGTGTCC AATCACATGT GTGGGTGCGA GGAGGCTGTG AGTCCGGGCG CTGCGACCGC
    TGTCAGAAAA AGATCCGGAT CTACCACAGT CTGACCGGGC TGCATTGTGT ATGGTGCCAC
    CTAGAGATCC ACGATGACTG CCTGCAAGCG GTGGGCCATG AGTGTGACTG TGGGCTGCTC
    CGGGATCACA TCCTGCCTCC ATCTTCCATC TATCCCAGTG TCCTGGCCTC TGGACCGGAT
    CGTAAAAATA GCAAAACAAG CCAGAAGACC ATGGATGATT TAAATTTGAG CACCTCTGAG
    GCTCTGCGGA TTGACCCTGT TCCTAACACC CACCCACTTC TCGTCTTTGT CAATCCTAAG
    AGTGGCGGGA AGCAGGGGCA AAGGGTGCTC TGGAAGTTCC AGTATATATT AAACCCTCGA
    CAGGTGTTCA ACCTCCTAAA GGATGGTCCT GAGATAGGGC TCCGATTATT CAAGGATGTT
    CCTGATAGCC GGATTTTGGT GTGTGGTGGA GACGGCACAG TAGGCTGGAT TCTAGAGACC
    ATTGACAAAG CTAACTTGCC AGTTTTGCCT CCTGTTGCTG TGTTGCCCCT GGGTACTGGA
    AATGATCTGG CTCGATGCCT AAGATGGGGA GGAGGTTATG AAGGACAGAA TCTGGCAAAG
    ATCCTCAAGG ATTTAGAGAT GAGTAAAGTG GTACATATGG ATCGATGGTC TGTGGAGGTG
    ATACCTCAAC AAACTGAAGA AAAAAGTGAC CCAGTCCCCT TTCAAATCAT CAATAACTAC
    TTCTCTATTG GCGTGGATGC CTCTATTGCT CATCGATTCC ACATCATGCG AGAGAAATAT
    CCGGAGAAGT TCAACAGCAG AATGAAGAAC AAGCTATGGT ACTTCGAATT TGCCACATCT
    GAATCCATCT TCTCAACATG CAAAAAGCTG GAGGAGTCTT TGACAGTTGA GATCTGTGGG
    AAACCGCTGG ATCTGAGCAA CCTGTCCCTA GAAGGCATCG CAGTGCTAAA CATCCCTAGC
    ATGCATGGTG GCTCCAACCT CTGGGGTGAT ACCAGGAGAC CCCATGGGGA TATCTATGGG
    ATCAACCAGG CCTTAGGTGC TACAGCTAAA GTCATCACCG ACCCTGATAT CCTGAAAACC
    TGTGTACCAG ACCTAAGTGA CAAGAGACTG GAAGTGGTTG GGCTGGAGGG TGCAATTGAG
    ATGGGCCAAA TCTATACCAA GCTCAAGAAT GCTGGACGTC GGCTGGCCAA GTGCTCTGAG
    ATCACCTTCC ACACCACAAA AACCCTTCCC ATGCAAATTG ACGGAGAACC CTGGATGCAG
    ACGCCCTGTA CAATCAAGAT CACCCACAAG AACCAGATGC CCATGCTCAT GGGCCCACCC
    CCCCGCTCCA CCAATTTCTT TGGCTTCTTG AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016874389.1
    CDS93..2768
    Translation

    Target ORF information:

    RefSeq Version XM_017018900.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018900.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGGGTCAT GGCCAGGAAG ACGCGCTAAC GCAGCCTACC CTCTGAAGAG GTCCAAGCAA 
    CGGAAGTACT ACTACGAAGC TGCCTTTCTG GCCATCCTTG AGAAAAATAG ACAGATGGCC
    AAGGAGAGGG GCCTAATAAG CCCCAGTGAT TTTGCCCAGC TGCAAAAATA CATGGAATAC
    TCCACCAAAA AGGTCAGTGA TGTCCTAAAG CTCTTCGAGG ATGGCGAGAT GGCTAAATAT
    GTCCAAGGAG ATGCCATTGG GTACGAGGGA TTCCAGCAAT TCCTGAAAAT CTATCTCGAA
    GTGGATAATG TTCCCAGACA CCTAAGCCTG GCACTGTTTC AATCCTTTGA GACTGGTCAC
    TGCTTAAATG AGACAAATGT GACAAAAGAT GTGGTGTGTC TCAATGATGT TTCCTGCTAC
    TTTTCCCTTC TGGAGGGTGG TCGGCCAGAA GACAAGTTAG AATTCACCTT CAAGCTGTAC
    GACACGGACA GAAATGGGAT CCTGGACAGC TCAGTGAGTT GGGGACCCTG TATGCTGGGC
    AAGGGAATAT GTGTTAATTT GTCTGCACCC TCCTGCCCCA ACTCTTCCAG GGTCTTAAGA
    AACAGAAGAG GATGGGGGGA GAGGTTGAGT CCTCTAGGAC TAAATTTGAA AGTAAGTCCC
    AAGCTCTTGA CCCCTCAAAG AGAATGCTGG ATACATGAAT TGGGTCTGCC TTACAAACAT
    ACAAACACAT TTATTTACTT GGCAGACATA CCTGAGAATG AGAGGTGTGA ATCTGGGGTG
    GAACCCAGGA ACACTGATTT CTGGAAAAGA GTTTGGATGG TGGTAGTAAA GGGGATATGG
    CTGTGGCTCT TGCCCTTTTT GCTCCAGGAA GTGGACAAAA TTATCCTACA GATGATGCGA
    GTGGCTGAAT ACCTGGATTG GGATGTGTCT GAGCTGAGGC CGATTCTTCA GGAGATGATG
    AAAGAGATTG ACTATGATGG CAGTGGCTCT GTCTCTCAAG CTGAGTGGGT CCGGGCTGGG
    GCCACCACCG TGCCACTGCT AGTGCTGCTG GGTCTGGAGA TGACTCTGAA GGACGACGGA
    CAGCACATGT GGAGGCCCAA GAGGTTCCCC AGACCAGTCT ACTGCAATCT GTGCGAGTCA
    AGCATTGGTC TTGGCAAACA GGGACTGAGC TGTAACCTCT GTAAGTACAC TGTTCACGAC
    CAGTGTGCCA TGAAAGCCCT GCCTTGTGAA GTCAGCACCT ATGCCAAGTC TCGGAAGGAC
    ATTGGTGTCC AATCACATGT GTGGGTGCGA GGAGGCTGTG AGTCCGGGCG CTGCGACCGC
    TGTCAGAAAA AGATCCGGAT CTACCACAGT CTGACCGGGC TGCATTGTGT ATGGTGCCAC
    CTAGAGATCC ACGATGACTG CCTGCAAGCG GTGGGCCATG AGTGTGACTG TGGGCTGCTC
    CGGGATCACA TCCTGCCTCC ATCTTCCATC TATCCCAGTG TCCTGGCCTC TGGACCGGAT
    CGTAAAAATA GCAAAACAAG CCAGAAGACC ATGGATGATT TAAATTTGAG CACCTCTGAG
    GCTCTGCGGA TTGACCCTGT TCCTAACACC CACCCACTTC TCGTCTTTGT CAATCCTAAG
    AGTGGCGGGA AGCAGGGGCA AAGGGTGCTC TGGAAGTTCC AGTATATATT AAACCCTCGA
    CAGGTGTTCA ACCTCCTAAA GGATGGTCCT GAGATAGGGC TCCGATTATT CAAGGATGTT
    CCTGATAGCC GGATTTTGGT GTGTGGTGGA GACGGCACAG TAGGCTGGAT TCTAGAGACC
    ATTGACAAAG CTAACTTGCC AGTTTTGCCT CCTGTTGCTG TGTTGCCCCT GGGTACTGGA
    AATGATCTGG CTCGATGCCT AAGATGGGGA GGAGGTTATG AAGGACAGAA TCTGGCAAAG
    ATCCTCAAGG ATTTAGAGAT GAGTAAAGTG GTACATATGG ATCGATGGTC TGTGGAGGTG
    ATACCTCAAC AAACTGAAGA AAAAAGTGAC CCAGTCCCCT TTCAAATCAT CAATAACTAC
    TTCTCTATTG GCGTGGATGC CTCTATTGCT CATCGATTCC ACATCATGCG AGAGAAATAT
    CCGGAGAAGT TCAACAGCAG AATGAAGAAC AAGCTATGGT ACTTCGAATT TGCCACATCT
    GAATCCATCT TCTCAACATG CAAAAAGCTG GAGGAGTCTT TGACAGTTGA GATCTGTGGG
    AAACCGCTGG ATCTGAGCAA CCTGTCCCTA GAAGGCATCG CAGTGCTAAA CATCCCTAGC
    ATGCATGGTG GCTCCAACCT CTGGGGTGAT ACCAGGAGAC CCCATGGGGA TATCTATGGG
    ATCAACCAGG CCTTAGGTGC TACAGCTAAA GTCATCACCG ACCCTGATAT CCTGAAAACC
    TGTGTACCAG ACCTAAGTGA CAAGAGACTG GAAGTGGTTG GGCTGGAGGG TGCAATTGAG
    ATGGGCCAAA TCTATACCAA GCTCAAGAAT GCTGGACGTC GGCTGGCCAA GTGCTCTGAG
    ATCACCTTCC ACACCACAAA AACCCTTCCC ATGCAAATTG ACGGAGAACC CTGGATGCAG
    ACGCCCTGTA CAATCAAGAT CACCCACAAG AACCAGATGC CCATGCTCAT GGGCCCACCC
    CCCCGCTCCA CCAATTTCTT TGGCTTCTTG AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu83568
    Clone ID Related Accession (Same CDS sequence) XM_017018903.1 , XM_017018904.1 , XM_017018901.2 , XM_017018907.1 , XM_017018906.1 , XM_017018905.1 , XM_017018906.2 , XM_017018901.1 , XM_017018902.1
    Accession Version XM_017018903.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2562bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG
    CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC
    TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA
    AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC
    AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT
    GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG
    ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG
    ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG
    ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG
    GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC
    GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC
    GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT
    CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG
    AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC
    GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG
    TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG
    CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA
    CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC
    TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT
    CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC
    CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG
    GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA
    GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT
    ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG
    GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG
    GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT
    AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG
    AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC
    ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC
    TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC
    CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC
    TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG
    AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA
    ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC
    TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG
    ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC
    CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016874392.1
    CDS375..2936
    Translation

    Target ORF information:

    RefSeq Version XM_017018903.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018903.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG
    CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC
    TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA
    AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC
    AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT
    GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG
    ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG
    ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG
    ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG
    GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC
    GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC
    GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT
    CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG
    AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC
    GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG
    TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG
    CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA
    CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC
    TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT
    CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC
    CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG
    GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA
    GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT
    ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG
    GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG
    GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT
    AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG
    AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC
    ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC
    TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC
    CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC
    TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG
    AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA
    ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC
    TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG
    ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC
    CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu83568
    Clone ID Related Accession (Same CDS sequence) XM_017018903.1 , XM_017018904.1 , XM_017018901.2 , XM_017018907.1 , XM_017018906.1 , XM_017018905.1 , XM_017018906.2 , XM_017018901.1 , XM_017018902.1
    Accession Version XM_017018904.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2562bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG
    CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC
    TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA
    AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC
    AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT
    GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG
    ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG
    ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG
    ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG
    GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC
    GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC
    GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT
    CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG
    AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC
    GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG
    TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG
    CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA
    CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC
    TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT
    CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC
    CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG
    GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA
    GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT
    ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG
    GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG
    GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT
    AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG
    AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC
    ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC
    TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC
    CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC
    TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG
    AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA
    ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC
    TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG
    ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC
    CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016874393.1
    CDS243..2804
    Translation

    Target ORF information:

    RefSeq Version XM_017018904.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018904.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG
    CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC
    TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA
    AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC
    AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT
    GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG
    ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG
    ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG
    ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG
    GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC
    GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC
    GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT
    CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG
    AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC
    GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG
    TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG
    CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA
    CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC
    TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT
    CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC
    CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG
    GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA
    GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT
    ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG
    GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG
    GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT
    AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG
    AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC
    ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC
    TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC
    CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC
    TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG
    AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA
    ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC
    TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG
    ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC
    CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu83568
    Clone ID Related Accession (Same CDS sequence) XM_017018903.1 , XM_017018904.1 , XM_017018901.2 , XM_017018907.1 , XM_017018906.1 , XM_017018905.1 , XM_017018906.2 , XM_017018901.1 , XM_017018902.1
    Accession Version XM_017018901.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2562bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017018901.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG
    CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC
    TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA
    AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC
    AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT
    GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG
    ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG
    ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG
    ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG
    GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC
    GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC
    GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT
    CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG
    AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC
    GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG
    TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG
    CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA
    CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC
    TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT
    CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC
    CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG
    GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA
    GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT
    ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG
    GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG
    GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT
    AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG
    AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC
    ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC
    TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC
    CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC
    TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG
    AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA
    ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC
    TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG
    ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC
    CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016874390.1
    CDS336..2897
    Translation

    Target ORF information:

    RefSeq Version XM_017018901.2
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018901.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG
    CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC
    TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA
    AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC
    AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT
    GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG
    ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG
    ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG
    ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG
    GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC
    GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC
    GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT
    CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG
    AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC
    GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG
    TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG
    CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA
    CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC
    TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT
    CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC
    CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG
    GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA
    GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT
    ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG
    GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG
    GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT
    AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG
    AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC
    ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC
    TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC
    CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC
    TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG
    AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA
    ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC
    TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG
    ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC
    CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu83568
    Clone ID Related Accession (Same CDS sequence) XM_017018903.1 , XM_017018904.1 , XM_017018901.2 , XM_017018907.1 , XM_017018906.1 , XM_017018905.1 , XM_017018906.2 , XM_017018901.1 , XM_017018902.1
    Accession Version XM_017018907.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2562bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG
    CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC
    TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA
    AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC
    AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT
    GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG
    ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG
    ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG
    ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG
    GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC
    GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC
    GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT
    CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG
    AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC
    GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG
    TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG
    CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA
    CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC
    TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT
    CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC
    CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG
    GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA
    GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT
    ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG
    GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG
    GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT
    AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG
    AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC
    ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC
    TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC
    CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC
    TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG
    AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA
    ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC
    TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG
    ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC
    CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016874396.1
    CDS107..2668
    Translation

    Target ORF information:

    RefSeq Version XM_017018907.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018907.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG
    CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC
    TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA
    AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC
    AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT
    GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG
    ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG
    ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG
    ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG
    GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC
    GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC
    GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT
    CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG
    AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC
    GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG
    TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG
    CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA
    CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC
    TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT
    CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC
    CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG
    GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA
    GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT
    ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG
    GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG
    GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT
    AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG
    AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC
    ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC
    TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC
    CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC
    TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG
    AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA
    ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC
    TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG
    ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC
    CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu83568
    Clone ID Related Accession (Same CDS sequence) XM_017018903.1 , XM_017018904.1 , XM_017018901.2 , XM_017018907.1 , XM_017018906.1 , XM_017018905.1 , XM_017018906.2 , XM_017018901.1 , XM_017018902.1
    Accession Version XM_017018906.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2562bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG
    CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC
    TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA
    AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC
    AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT
    GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG
    ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG
    ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG
    ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG
    GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC
    GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC
    GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT
    CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG
    AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC
    GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG
    TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG
    CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA
    CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC
    TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT
    CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC
    CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG
    GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA
    GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT
    ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG
    GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG
    GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT
    AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG
    AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC
    ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC
    TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC
    CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC
    TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG
    AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA
    ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC
    TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG
    ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC
    CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016874395.1
    CDS284..2845
    Translation

    Target ORF information:

    RefSeq Version XM_017018906.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018906.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG
    CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC
    TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA
    AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC
    AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT
    GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG
    ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG
    ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG
    ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG
    GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC
    GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC
    GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT
    CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG
    AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC
    GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG
    TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG
    CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA
    CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC
    TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT
    CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC
    CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG
    GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA
    GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT
    ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG
    GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG
    GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT
    AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG
    AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC
    ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC
    TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC
    CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC
    TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG
    AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA
    ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC
    TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG
    ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC
    CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu83568
    Clone ID Related Accession (Same CDS sequence) XM_017018903.1 , XM_017018904.1 , XM_017018901.2 , XM_017018907.1 , XM_017018906.1 , XM_017018905.1 , XM_017018906.2 , XM_017018901.1 , XM_017018902.1
    Accession Version XM_017018905.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2562bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG
    CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC
    TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA
    AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC
    AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT
    GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG
    ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG
    ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG
    ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG
    GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC
    GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC
    GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT
    CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG
    AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC
    GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG
    TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG
    CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA
    CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC
    TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT
    CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC
    CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG
    GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA
    GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT
    ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG
    GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG
    GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT
    AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG
    AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC
    ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC
    TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC
    CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC
    TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG
    AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA
    ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC
    TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG
    ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC
    CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016874394.1
    CDS652..3213
    Translation

    Target ORF information:

    RefSeq Version XM_017018905.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018905.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG
    CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC
    TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA
    AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC
    AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT
    GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG
    ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG
    ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG
    ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG
    GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC
    GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC
    GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT
    CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG
    AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC
    GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG
    TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG
    CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA
    CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC
    TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT
    CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC
    CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG
    GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA
    GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT
    ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG
    GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG
    GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT
    AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG
    AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC
    ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC
    TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC
    CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC
    TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG
    AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA
    ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC
    TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG
    ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC
    CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu83568
    Clone ID Related Accession (Same CDS sequence) XM_017018903.1 , XM_017018904.1 , XM_017018901.2 , XM_017018907.1 , XM_017018906.1 , XM_017018905.1 , XM_017018906.2 , XM_017018901.1 , XM_017018902.1
    Accession Version XM_017018906.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2562bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017018906.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG
    CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC
    TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA
    AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC
    AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT
    GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG
    ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG
    ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG
    ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG
    GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC
    GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC
    GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT
    CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG
    AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC
    GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG
    TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG
    CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA
    CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC
    TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT
    CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC
    CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG
    GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA
    GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT
    ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG
    GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG
    GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT
    AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG
    AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC
    ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC
    TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC
    CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC
    TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG
    AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA
    ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC
    TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG
    ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC
    CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016874395.1
    CDS275..2836
    Translation

    Target ORF information:

    RefSeq Version XM_017018906.2
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018906.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG
    CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC
    TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA
    AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC
    AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT
    GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG
    ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG
    ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG
    ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG
    GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC
    GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC
    GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT
    CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG
    AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC
    GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG
    TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG
    CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA
    CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC
    TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT
    CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC
    CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG
    GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA
    GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT
    ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG
    GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG
    GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT
    AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG
    AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC
    ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC
    TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC
    CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC
    TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG
    AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA
    ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC
    TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG
    ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC
    CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu83568
    Clone ID Related Accession (Same CDS sequence) XM_017018903.1 , XM_017018904.1 , XM_017018901.2 , XM_017018907.1 , XM_017018906.1 , XM_017018905.1 , XM_017018906.2 , XM_017018901.1 , XM_017018902.1
    Accession Version XM_017018901.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2562bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG
    CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC
    TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA
    AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC
    AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT
    GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG
    ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG
    ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG
    ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG
    GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC
    GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC
    GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT
    CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG
    AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC
    GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG
    TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG
    CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA
    CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC
    TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT
    CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC
    CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG
    GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA
    GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT
    ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG
    GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG
    GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT
    AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG
    AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC
    ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC
    TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC
    CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC
    TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG
    AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA
    ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC
    TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG
    ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC
    CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016874390.1
    CDS351..2912
    Translation

    Target ORF information:

    RefSeq Version XM_017018901.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018901.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG
    CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC
    TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA
    AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC
    AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT
    GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG
    ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG
    ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG
    ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG
    GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC
    GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC
    GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT
    CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG
    AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC
    GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG
    TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG
    CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA
    CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC
    TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT
    CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC
    CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG
    GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA
    GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT
    ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG
    GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG
    GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT
    AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG
    AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC
    ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC
    TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC
    CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC
    TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG
    AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA
    ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC
    TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG
    ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC
    CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu83568
    Clone ID Related Accession (Same CDS sequence) XM_017018903.1 , XM_017018904.1 , XM_017018901.2 , XM_017018907.1 , XM_017018906.1 , XM_017018905.1 , XM_017018906.2 , XM_017018901.1 , XM_017018902.1
    Accession Version XM_017018902.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2562bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG
    CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC
    TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA
    AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC
    AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT
    GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG
    ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG
    ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG
    ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG
    GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC
    GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC
    GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT
    CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG
    AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC
    GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG
    TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG
    CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA
    CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC
    TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT
    CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC
    CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG
    GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA
    GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT
    ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG
    GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG
    GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT
    AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG
    AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC
    ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC
    TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC
    CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC
    TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG
    AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA
    ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC
    TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG
    ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC
    CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016874391.1
    CDS173..2734
    Translation

    Target ORF information:

    RefSeq Version XM_017018902.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018902.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT
    AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT
    CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT
    GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC
    TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG
    CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG
    CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC
    TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA
    AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC
    AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT
    GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG
    ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG
    ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG
    ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG
    GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC
    GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC
    GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT
    CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG
    AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC
    GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG
    TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG
    CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA
    CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC
    TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT
    CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC
    CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG
    GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA
    GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT
    ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG
    GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG
    GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT
    AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG
    AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC
    ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC
    TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC
    CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC
    TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG
    AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA
    ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC
    TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG
    ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC
    CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu83569
    Clone ID Related Accession (Same CDS sequence) XM_017018908.1
    Accession Version XM_017018908.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2460bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product diacylglycerol kinase alpha isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGGGTCAT GGCCAGGAAG ACGCGCTAAC GCAGCCTACC CTCTGAAGAG GTCCAAGCAA 
    CGGAAGTACT ACTACGAAGC TGCCTTTCTG GCCATCCTTG AGAAAAATAG ACAGATGGCC
    AAGGAGAGGG GCCTAATAAG CCCCAGTGAT TTTGCCCAGC TGCAAAAATA CATGGAATAC
    TCCACCAAAA AGGTCAGTGA TGTCCTAAAG CTCTTCGAGG ATGGCGAGAT GGCTAAATAT
    GTCCAAGGAG ATGCCATTGG GTACGAGGGA TTCCAGCAAT TCCTGAAAAT CTATCTCGAA
    GTGGATAATG TTCCCAGACA CCTAAGCCTG GCACTGTTTC AATCCTTTGA GACTGGTCAC
    TGCTTAAATG AGACAAATGT GACAAAAGAT GTGGTGTGTC TCAATGATGT TTCCTGCTAC
    TTTTCCCTTC TGGAGGGTGG TCGGCCAGAA GACAAGTTAG AATTCACCTT CAAGCTGTAC
    GACACGGACA GAAATGGGAT CCTGGACAGC TCAGTGAGTT GGGGACCCTG TATGCTGGGC
    AAGGGAATAT GTGTTAATTT GTCTGCACCC TCCTGCCCCA ACTCTTCCAG GGTCTTAAGA
    AACAGAAGAG GATGGGGGGA GAGGTTGAGT CCTCTAGGAC TAAATTTGAA AGAAGTGGAC
    AAAATTATCC TACAGATGAT GCGAGTGGCT GAATACCTGG ATTGGGATGT GTCTGAGCTG
    AGGCCGATTC TTCAGGAGAT GATGAAAGAG ATTGACTATG ATGGCAGTGG CTCTGTCTCT
    CAAGCTGAGT GGGTCCGGGC TGGGGCCACC ACCGTGCCAC TGCTAGTGCT GCTGGGTCTG
    GAGATGACTC TGAAGGACGA CGGACAGCAC ATGTGGAGGC CCAAGAGGTT CCCCAGACCA
    GTCTACTGCA ATCTGTGCGA GTCAAGCATT GGTCTTGGCA AACAGGGACT GAGCTGTAAC
    CTCTGTAAGT ACACTGTTCA CGACCAGTGT GCCATGAAAG CCCTGCCTTG TGAAGTCAGC
    ACCTATGCCA AGTCTCGGAA GGACATTGGT GTCCAATCAC ATGTGTGGGT GCGAGGAGGC
    TGTGAGTCCG GGCGCTGCGA CCGCTGTCAG AAAAAGATCC GGATCTACCA CAGTCTGACC
    GGGCTGCATT GTGTATGGTG CCACCTAGAG ATCCACGATG ACTGCCTGCA AGCGGTGGGC
    CATGAGTGTG ACTGTGGGCT GCTCCGGGAT CACATCCTGC CTCCATCTTC CATCTATCCC
    AGTGTCCTGG CCTCTGGACC GGATCGTAAA AATAGCAAAA CAAGCCAGAA GACCATGGAT
    GATTTAAATT TGAGCACCTC TGAGGCTCTG CGGATTGACC CTGTTCCTAA CACCCACCCA
    CTTCTCGTCT TTGTCAATCC TAAGAGTGGC GGGAAGCAGG GGCAAAGGGT GCTCTGGAAG
    TTCCAGTATA TATTAAACCC TCGACAGGTG TTCAACCTCC TAAAGGATGG TCCTGAGATA
    GGGCTCCGAT TATTCAAGGA TGTTCCTGAT AGCCGGATTT TGGTGTGTGG TGGAGACGGC
    ACAGTAGGCT GGATTCTAGA GACCATTGAC AAAGCTAACT TGCCAGTTTT GCCTCCTGTT
    GCTGTGTTGC CCCTGGGTAC TGGAAATGAT CTGGCTCGAT GCCTAAGATG GGGAGGAGGT
    TATGAAGGAC AGAATCTGGC AAAGATCCTC AAGGATTTAG AGATGAGTAA AGTGGTACAT
    ATGGATCGAT GGTCTGTGGA GGTGATACCT CAACAAACTG AAGAAAAAAG TGACCCAGTC
    CCCTTTCAAA TCATCAATAA CTACTTCTCT ATTGGCGTGG ATGCCTCTAT TGCTCATCGA
    TTCCACATCA TGCGAGAGAA ATATCCGGAG AAGTTCAACA GCAGAATGAA GAACAAGCTA
    TGGTACTTCG AATTTGCCAC ATCTGAATCC ATCTTCTCAA CATGCAAAAA GCTGGAGGAG
    TCTTTGACAG TTGAGATCTG TGGGAAACCG CTGGATCTGA GCAACCTGTC CCTAGAAGGC
    ATCGCAGTGC TAAACATCCC TAGCATGCAT GGTGGCTCCA ACCTCTGGGG TGATACCAGG
    AGACCCCATG GGGATATCTA TGGGATCAAC CAGGCCTTAG GTGCTACAGC TAAAGTCATC
    ACCGACCCTG ATATCCTGAA AACCTGTGTA CCAGACCTAA GTGACAAGAG ACTGGAAGTG
    GTTGGGCTGG AGGGTGCAAT TGAGATGGGC CAAATCTATA CCAAGCTCAA GAATGCTGGA
    CGTCGGCTGG CCAAGTGCTC TGAGATCACC TTCCACACCA CAAAAACCCT TCCCATGCAA
    ATTGACGGAG AACCCTGGAT GCAGACGCCC TGTACAATCA AGATCACCCA CAAGAACCAG
    ATGCCCATGC TCATGGGCCC ACCCCCCCGC TCCACCAATT TCTTTGGCTT CTTGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016874397.1
    CDS93..2552
    Translation

    Target ORF information:

    RefSeq Version XM_017018908.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018908.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGGGTCAT GGCCAGGAAG ACGCGCTAAC GCAGCCTACC CTCTGAAGAG GTCCAAGCAA 
    CGGAAGTACT ACTACGAAGC TGCCTTTCTG GCCATCCTTG AGAAAAATAG ACAGATGGCC
    AAGGAGAGGG GCCTAATAAG CCCCAGTGAT TTTGCCCAGC TGCAAAAATA CATGGAATAC
    TCCACCAAAA AGGTCAGTGA TGTCCTAAAG CTCTTCGAGG ATGGCGAGAT GGCTAAATAT
    GTCCAAGGAG ATGCCATTGG GTACGAGGGA TTCCAGCAAT TCCTGAAAAT CTATCTCGAA
    GTGGATAATG TTCCCAGACA CCTAAGCCTG GCACTGTTTC AATCCTTTGA GACTGGTCAC
    TGCTTAAATG AGACAAATGT GACAAAAGAT GTGGTGTGTC TCAATGATGT TTCCTGCTAC
    TTTTCCCTTC TGGAGGGTGG TCGGCCAGAA GACAAGTTAG AATTCACCTT CAAGCTGTAC
    GACACGGACA GAAATGGGAT CCTGGACAGC TCAGTGAGTT GGGGACCCTG TATGCTGGGC
    AAGGGAATAT GTGTTAATTT GTCTGCACCC TCCTGCCCCA ACTCTTCCAG GGTCTTAAGA
    AACAGAAGAG GATGGGGGGA GAGGTTGAGT CCTCTAGGAC TAAATTTGAA AGAAGTGGAC
    AAAATTATCC TACAGATGAT GCGAGTGGCT GAATACCTGG ATTGGGATGT GTCTGAGCTG
    AGGCCGATTC TTCAGGAGAT GATGAAAGAG ATTGACTATG ATGGCAGTGG CTCTGTCTCT
    CAAGCTGAGT GGGTCCGGGC TGGGGCCACC ACCGTGCCAC TGCTAGTGCT GCTGGGTCTG
    GAGATGACTC TGAAGGACGA CGGACAGCAC ATGTGGAGGC CCAAGAGGTT CCCCAGACCA
    GTCTACTGCA ATCTGTGCGA GTCAAGCATT GGTCTTGGCA AACAGGGACT GAGCTGTAAC
    CTCTGTAAGT ACACTGTTCA CGACCAGTGT GCCATGAAAG CCCTGCCTTG TGAAGTCAGC
    ACCTATGCCA AGTCTCGGAA GGACATTGGT GTCCAATCAC ATGTGTGGGT GCGAGGAGGC
    TGTGAGTCCG GGCGCTGCGA CCGCTGTCAG AAAAAGATCC GGATCTACCA CAGTCTGACC
    GGGCTGCATT GTGTATGGTG CCACCTAGAG ATCCACGATG ACTGCCTGCA AGCGGTGGGC
    CATGAGTGTG ACTGTGGGCT GCTCCGGGAT CACATCCTGC CTCCATCTTC CATCTATCCC
    AGTGTCCTGG CCTCTGGACC GGATCGTAAA AATAGCAAAA CAAGCCAGAA GACCATGGAT
    GATTTAAATT TGAGCACCTC TGAGGCTCTG CGGATTGACC CTGTTCCTAA CACCCACCCA
    CTTCTCGTCT TTGTCAATCC TAAGAGTGGC GGGAAGCAGG GGCAAAGGGT GCTCTGGAAG
    TTCCAGTATA TATTAAACCC TCGACAGGTG TTCAACCTCC TAAAGGATGG TCCTGAGATA
    GGGCTCCGAT TATTCAAGGA TGTTCCTGAT AGCCGGATTT TGGTGTGTGG TGGAGACGGC
    ACAGTAGGCT GGATTCTAGA GACCATTGAC AAAGCTAACT TGCCAGTTTT GCCTCCTGTT
    GCTGTGTTGC CCCTGGGTAC TGGAAATGAT CTGGCTCGAT GCCTAAGATG GGGAGGAGGT
    TATGAAGGAC AGAATCTGGC AAAGATCCTC AAGGATTTAG AGATGAGTAA AGTGGTACAT
    ATGGATCGAT GGTCTGTGGA GGTGATACCT CAACAAACTG AAGAAAAAAG TGACCCAGTC
    CCCTTTCAAA TCATCAATAA CTACTTCTCT ATTGGCGTGG ATGCCTCTAT TGCTCATCGA
    TTCCACATCA TGCGAGAGAA ATATCCGGAG AAGTTCAACA GCAGAATGAA GAACAAGCTA
    TGGTACTTCG AATTTGCCAC ATCTGAATCC ATCTTCTCAA CATGCAAAAA GCTGGAGGAG
    TCTTTGACAG TTGAGATCTG TGGGAAACCG CTGGATCTGA GCAACCTGTC CCTAGAAGGC
    ATCGCAGTGC TAAACATCCC TAGCATGCAT GGTGGCTCCA ACCTCTGGGG TGATACCAGG
    AGACCCCATG GGGATATCTA TGGGATCAAC CAGGCCTTAG GTGCTACAGC TAAAGTCATC
    ACCGACCCTG ATATCCTGAA AACCTGTGTA CCAGACCTAA GTGACAAGAG ACTGGAAGTG
    GTTGGGCTGG AGGGTGCAAT TGAGATGGGC CAAATCTATA CCAAGCTCAA GAATGCTGGA
    CGTCGGCTGG CCAAGTGCTC TGAGATCACC TTCCACACCA CAAAAACCCT TCCCATGCAA
    ATTGACGGAG AACCCTGGAT GCAGACGCCC TGTACAATCA AGATCACCCA CAAGAACCAG
    ATGCCCATGC TCATGGGCCC ACCCCCCCGC TCCACCAATT TCTTTGGCTT CTTGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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