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SPRED1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol SPRED1
Entrez Gene ID 161742
Full Name sprouty-related, EVH1 domain containing 1
Synonyms NFLS, PPP1R147, hSpred1, spred-1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

15

15q14

Summary The protein encoded by this gene is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. The encoded protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

609291

Disorder Html: Legius syndrome, 611431 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_005254202 XP_005254259 sprouty-related, EVH1 domain-containing protein 1 isoform X1
XM_011521288 XP_011519590 sprouty-related, EVH1 domain-containing protein 1 isoform X2
XM_011521289 XP_011519591 sprouty-related, EVH1 domain-containing protein 1 isoform X2
XM_005254203 XP_005254260 sprouty-related, EVH1 domain-containing protein 1 isoform X3
XM_011521290 XP_011519592 sprouty-related, EVH1 domain-containing protein 1 isoform X2
NM_152594 NP_689807 sprouty-related, EVH1 domain-containing protein 1



Homo sapiens (human) SPRED1 NP_689807.1
Pan troglodytes (chimpanzee) SPRED1 XP_001138171.1
Canis lupus familiaris (dog) SPRED1 XP_544609.3
Bos taurus (cattle) SPRED1 NP_001179445.1
Mus musculus (house mouse) Spred1 NP_277059.1
Rattus norvegicus (Norway rat) Spred1 NP_001040554.1
Gallus gallus (chicken) SPRED1 NP_001186638.1
Danio rerio (zebrafish) spred1 NP_998397.1
Xenopus (Silurana) tropicalis (western clawed frog) spred1 NP_001008037.1


The following SPRED1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPRED1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu48143 XM_005254202 PREDICTED: Homo sapiens sprouty-related, EVH1 domain containing 1 (SPRED1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu77141 XM_011521288 PREDICTED: Homo sapiens sprouty-related, EVH1 domain containing 1 (SPRED1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu77141 XM_011521289 PREDICTED: Homo sapiens sprouty-related, EVH1 domain containing 1 (SPRED1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu48144 XM_005254203 PREDICTED: Homo sapiens sprouty-related, EVH1 domain containing 1 (SPRED1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu77141 XM_011521290 PREDICTED: Homo sapiens sprouty-related, EVH1 domain containing 1 (SPRED1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu18529 NM_152594 Homo sapiens sprouty-related, EVH1 domain containing 1 (SPRED1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu48143
Clone ID Related Accession (Same CDS sequence) XM_005254202
Accession Version XM_005254202.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1371bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product sprouty-related, EVH1 domain-containing protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530405472. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGCGAGG AGACGGCGAC TTCTGACAAC GAGTGTCAGG GGCATGATGA TGGAATCTGC 
TTTTTTCATA ATAGTTATGC ACGAGTGCGA GCTGTGGTGA TGACCCGAGA TGACTCAAGT
GGTGGATGGT TACCACTTGG AGGGAGTGGA CTAAGCAGCG TCACTGTCTT CAAAGTCCCT
CATCAGGAAG AGAATGGCTG TGCTGACTTT TTTATCCGTG GAGAGCGACT CAGGGACAAA
ATGGTGGTTT TGGAATGTAT GCTTAAAAAA GACCTCATTT ATAATAAGGT CACTCCAACA
TTTCACCACT GGAAGATTGA TGACAAGAAG TTTGGTCTTA CGTTTCAAAG TCCTGCTGAT
GCTAGGGCTT TTGATAGAGG TATCCGAAGA GCTATAGAGG ATATTTCTCA AGGATGCCCC
GAATCAAAAA ATGAAGCTGA AGGGGCAGAT GACTTACAAG CAAATGAAGA GGATTCTTCC
AGTTCTCTAG TGAAGGATCA CCTTTTTCAG CAAGAGACAG TTGTTACCAG TGAGCCTTAT
AGAAGCTCAA ATATAAGACC TTCTCCCTTT GAAGATCTGA ATGCCAGAAG AGTCTACATG
CAAAGCCAAG CCAATCAGAT AACATTTGGT CAGCCAGGCT TGGACATTCA GAGCAGAAGT
ATGGAATACG TACAGCGGCA AATATCCAAG GAATGTGGAA GCCTAAAGTC CCAAAATAGG
GTCCCTTTGA AATCAATCAG ACATGTCAGC TTTCAAGATG AGGATGAGAT TGTCAGAATA
AACCCTCGAG ATATCTTAAT ACGTCGCTAT GCAGACTACA GACATCCTGA CATGTGGAAA
AATGACTTGG AAAGAGATGA TGCTGATTCC AGTATTCAGT TTTCTAAACC AGACAGTAAA
AAATCAGACT ATCTGTACTC TTGTGGGGAT GAGACTAAGT TAAGTTCACC CAAAGACTCT
GTGGTATTTA AGACGCAGCC TTCCTCATTA AAAATTAAGA AGTCAAAACG AAGAAAAGAG
GATGGTGAAC GTTCTCGCTG CGTATACTGC CAGGAAAGGT TTAATCATGA AGAAAATGTT
AGGGGAAAAT GTCAGGATGC TCCAGACCCT ATTAAAAGAT GCATATATCA AGTTAGTTGC
ATGCTCTGTG CAGAGAGCAT GTTGTATCAT TGTATGTCAG ACTCAGAGGG AGATTTTTCT
GATCCCTGTT CGTGTGACAC TAGCGACGAC AAGTTCTGCT TGCGATGGTT AGCCCTGGTA
GCTTTGTCTT TCATTGTACC ATGTATGTGC TGCTACGTCC CTTTGAGAAT GTGCCATCGC
TGTGGTGAGG CATGTGGTTG CTGTGGTGGG AAACATAAAG CTGCTGGATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254259.1
CDS1100..2470
Misc Feature(1)1172..1510(+)
Misc Feature(2)1199..1408(+)
Misc Feature(3)2129..2419(+)
Translation

Target ORF information:

RefSeq Version XM_005254202
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens sprouty-related, EVH1 domain containing 1 (SPRED1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254202

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGCGAGG AGACGGCGAC TTCTGACAAC GAGTGTCAGG GGCATGATGA TGGAATCTGC 
TTTTTTCATA ATAGTTATGC ACGAGTGCGA GCTGTGGTGA TGACCCGAGA TGACTCAAGT
GGTGGATGGT TACCACTTGG AGGGAGTGGA CTAAGCAGCG TCACTGTCTT CAAAGTCCCT
CATCAGGAAG AGAATGGCTG TGCTGACTTT TTTATCCGTG GAGAGCGACT CAGGGACAAA
ATGGTGGTTT TGGAATGTAT GCTTAAAAAA GACCTCATTT ATAATAAGGT CACTCCAACA
TTTCACCACT GGAAGATTGA TGACAAGAAG TTTGGTCTTA CGTTTCAAAG TCCTGCTGAT
GCTAGGGCTT TTGATAGAGG TATCCGAAGA GCTATAGAGG ATATTTCTCA AGGATGCCCC
GAATCAAAAA ATGAAGCTGA AGGGGCAGAT GACTTACAAG CAAATGAAGA GGATTCTTCC
AGTTCTCTAG TGAAGGATCA CCTTTTTCAG CAAGAGACAG TTGTTACCAG TGAGCCTTAT
AGAAGCTCAA ATATAAGACC TTCTCCCTTT GAAGATCTGA ATGCCAGAAG AGTCTACATG
CAAAGCCAAG CCAATCAGAT AACATTTGGT CAGCCAGGCT TGGACATTCA GAGCAGAAGT
ATGGAATACG TACAGCGGCA AATATCCAAG GAATGTGGAA GCCTAAAGTC CCAAAATAGG
GTCCCTTTGA AATCAATCAG ACATGTCAGC TTTCAAGATG AGGATGAGAT TGTCAGAATA
AACCCTCGAG ATATCTTAAT ACGTCGCTAT GCAGACTACA GACATCCTGA CATGTGGAAA
AATGACTTGG AAAGAGATGA TGCTGATTCC AGTATTCAGT TTTCTAAACC AGACAGTAAA
AAATCAGACT ATCTGTACTC TTGTGGGGAT GAGACTAAGT TAAGTTCACC CAAAGACTCT
GTGGTATTTA AGACGCAGCC TTCCTCATTA AAAATTAAGA AGTCAAAACG AAGAAAAGAG
GATGGTGAAC GTTCTCGCTG CGTATACTGC CAGGAAAGGT TTAATCATGA AGAAAATGTT
AGGGGAAAAT GTCAGGATGC TCCAGACCCT ATTAAAAGAT GCATATATCA AGTTAGTTGC
ATGCTCTGTG CAGAGAGCAT GTTGTATCAT TGTATGTCAG ACTCAGAGGG AGATTTTTCT
GATCCCTGTT CGTGTGACAC TAGCGACGAC AAGTTCTGCT TGCGATGGTT AGCCCTGGTA
GCTTTGTCTT TCATTGTACC ATGTATGTGC TGCTACGTCC CTTTGAGAAT GTGCCATCGC
TGTGGTGAGG CATGTGGTTG CTGTGGTGGG AAACATAAAG CTGCTGGATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu77141
Clone ID Related Accession (Same CDS sequence) XM_011521288 , XM_011521289 , XM_011521290
Accession Version XM_011521288.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1272bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product sprouty-related, EVH1 domain-containing protein 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACCCGAG ATGACTCAAG TGGTGGATGG TTACCACTTG GAGGGAGTGG ACTAAGCAGC 
GTCACTGTCT TCAAAGTCCC TCATCAGGAA GAGAATGGCT GTGCTGACTT TTTTATCCGT
GGAGAGCGAC TCAGGGACAA AATGGTGGTT TTGGAATGTA TGCTTAAAAA AGACCTCATT
TATAATAAGG TCACTCCAAC ATTTCACCAC TGGAAGATTG ATGACAAGAA GTTTGGTCTT
ACGTTTCAAA GTCCTGCTGA TGCTAGGGCT TTTGATAGAG GTATCCGAAG AGCTATAGAG
GATATTTCTC AAGGATGCCC CGAATCAAAA AATGAAGCTG AAGGGGCAGA TGACTTACAA
GCAAATGAAG AGGATTCTTC CAGTTCTCTA GTGAAGGATC ACCTTTTTCA GCAAGAGACA
GTTGTTACCA GTGAGCCTTA TAGAAGCTCA AATATAAGAC CTTCTCCCTT TGAAGATCTG
AATGCCAGAA GAGTCTACAT GCAAAGCCAA GCCAATCAGA TAACATTTGG TCAGCCAGGC
TTGGACATTC AGAGCAGAAG TATGGAATAC GTACAGCGGC AAATATCCAA GGAATGTGGA
AGCCTAAAGT CCCAAAATAG GGTCCCTTTG AAATCAATCA GACATGTCAG CTTTCAAGAT
GAGGATGAGA TTGTCAGAAT AAACCCTCGA GATATCTTAA TACGTCGCTA TGCAGACTAC
AGACATCCTG ACATGTGGAA AAATGACTTG GAAAGAGATG ATGCTGATTC CAGTATTCAG
TTTTCTAAAC CAGACAGTAA AAAATCAGAC TATCTGTACT CTTGTGGGGA TGAGACTAAG
TTAAGTTCAC CCAAAGACTC TGTGGTATTT AAGACGCAGC CTTCCTCATT AAAAATTAAG
AAGTCAAAAC GAAGAAAAGA GGATGGTGAA CGTTCTCGCT GCGTATACTG CCAGGAAAGG
TTTAATCATG AAGAAAATGT TAGGGGAAAA TGTCAGGATG CTCCAGACCC TATTAAAAGA
TGCATATATC AAGTTAGTTG CATGCTCTGT GCAGAGAGCA TGTTGTATCA TTGTATGTCA
GACTCAGAGG GAGATTTTTC TGATCCCTGT TCGTGTGACA CTAGCGACGA CAAGTTCTGC
TTGCGATGGT TAGCCCTGGT AGCTTTGTCT TTCATTGTAC CATGTATGTG CTGCTACGTC
CCTTTGAGAA TGTGCCATCG CTGTGGTGAG GCATGTGGTT GCTGTGGTGG GAAACATAAA
GCTGCTGGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011519590.1
CDS81..1352
Misc Feature(1)81..392(+)
Misc Feature(2)81..290(+)
Misc Feature(3)1011..1301(+)
Translation

Target ORF information:

RefSeq Version XM_011521288
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens sprouty-related, EVH1 domain containing 1 (SPRED1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011521288

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACCCGAG ATGACTCAAG TGGTGGATGG TTACCACTTG GAGGGAGTGG ACTAAGCAGC 
GTCACTGTCT TCAAAGTCCC TCATCAGGAA GAGAATGGCT GTGCTGACTT TTTTATCCGT
GGAGAGCGAC TCAGGGACAA AATGGTGGTT TTGGAATGTA TGCTTAAAAA AGACCTCATT
TATAATAAGG TCACTCCAAC ATTTCACCAC TGGAAGATTG ATGACAAGAA GTTTGGTCTT
ACGTTTCAAA GTCCTGCTGA TGCTAGGGCT TTTGATAGAG GTATCCGAAG AGCTATAGAG
GATATTTCTC AAGGATGCCC CGAATCAAAA AATGAAGCTG AAGGGGCAGA TGACTTACAA
GCAAATGAAG AGGATTCTTC CAGTTCTCTA GTGAAGGATC ACCTTTTTCA GCAAGAGACA
GTTGTTACCA GTGAGCCTTA TAGAAGCTCA AATATAAGAC CTTCTCCCTT TGAAGATCTG
AATGCCAGAA GAGTCTACAT GCAAAGCCAA GCCAATCAGA TAACATTTGG TCAGCCAGGC
TTGGACATTC AGAGCAGAAG TATGGAATAC GTACAGCGGC AAATATCCAA GGAATGTGGA
AGCCTAAAGT CCCAAAATAG GGTCCCTTTG AAATCAATCA GACATGTCAG CTTTCAAGAT
GAGGATGAGA TTGTCAGAAT AAACCCTCGA GATATCTTAA TACGTCGCTA TGCAGACTAC
AGACATCCTG ACATGTGGAA AAATGACTTG GAAAGAGATG ATGCTGATTC CAGTATTCAG
TTTTCTAAAC CAGACAGTAA AAAATCAGAC TATCTGTACT CTTGTGGGGA TGAGACTAAG
TTAAGTTCAC CCAAAGACTC TGTGGTATTT AAGACGCAGC CTTCCTCATT AAAAATTAAG
AAGTCAAAAC GAAGAAAAGA GGATGGTGAA CGTTCTCGCT GCGTATACTG CCAGGAAAGG
TTTAATCATG AAGAAAATGT TAGGGGAAAA TGTCAGGATG CTCCAGACCC TATTAAAAGA
TGCATATATC AAGTTAGTTG CATGCTCTGT GCAGAGAGCA TGTTGTATCA TTGTATGTCA
GACTCAGAGG GAGATTTTTC TGATCCCTGT TCGTGTGACA CTAGCGACGA CAAGTTCTGC
TTGCGATGGT TAGCCCTGGT AGCTTTGTCT TTCATTGTAC CATGTATGTG CTGCTACGTC
CCTTTGAGAA TGTGCCATCG CTGTGGTGAG GCATGTGGTT GCTGTGGTGG GAAACATAAA
GCTGCTGGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu77141
Clone ID Related Accession (Same CDS sequence) XM_011521288 , XM_011521289 , XM_011521290
Accession Version XM_011521289.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1272bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product sprouty-related, EVH1 domain-containing protein 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACCCGAG ATGACTCAAG TGGTGGATGG TTACCACTTG GAGGGAGTGG ACTAAGCAGC 
GTCACTGTCT TCAAAGTCCC TCATCAGGAA GAGAATGGCT GTGCTGACTT TTTTATCCGT
GGAGAGCGAC TCAGGGACAA AATGGTGGTT TTGGAATGTA TGCTTAAAAA AGACCTCATT
TATAATAAGG TCACTCCAAC ATTTCACCAC TGGAAGATTG ATGACAAGAA GTTTGGTCTT
ACGTTTCAAA GTCCTGCTGA TGCTAGGGCT TTTGATAGAG GTATCCGAAG AGCTATAGAG
GATATTTCTC AAGGATGCCC CGAATCAAAA AATGAAGCTG AAGGGGCAGA TGACTTACAA
GCAAATGAAG AGGATTCTTC CAGTTCTCTA GTGAAGGATC ACCTTTTTCA GCAAGAGACA
GTTGTTACCA GTGAGCCTTA TAGAAGCTCA AATATAAGAC CTTCTCCCTT TGAAGATCTG
AATGCCAGAA GAGTCTACAT GCAAAGCCAA GCCAATCAGA TAACATTTGG TCAGCCAGGC
TTGGACATTC AGAGCAGAAG TATGGAATAC GTACAGCGGC AAATATCCAA GGAATGTGGA
AGCCTAAAGT CCCAAAATAG GGTCCCTTTG AAATCAATCA GACATGTCAG CTTTCAAGAT
GAGGATGAGA TTGTCAGAAT AAACCCTCGA GATATCTTAA TACGTCGCTA TGCAGACTAC
AGACATCCTG ACATGTGGAA AAATGACTTG GAAAGAGATG ATGCTGATTC CAGTATTCAG
TTTTCTAAAC CAGACAGTAA AAAATCAGAC TATCTGTACT CTTGTGGGGA TGAGACTAAG
TTAAGTTCAC CCAAAGACTC TGTGGTATTT AAGACGCAGC CTTCCTCATT AAAAATTAAG
AAGTCAAAAC GAAGAAAAGA GGATGGTGAA CGTTCTCGCT GCGTATACTG CCAGGAAAGG
TTTAATCATG AAGAAAATGT TAGGGGAAAA TGTCAGGATG CTCCAGACCC TATTAAAAGA
TGCATATATC AAGTTAGTTG CATGCTCTGT GCAGAGAGCA TGTTGTATCA TTGTATGTCA
GACTCAGAGG GAGATTTTTC TGATCCCTGT TCGTGTGACA CTAGCGACGA CAAGTTCTGC
TTGCGATGGT TAGCCCTGGT AGCTTTGTCT TTCATTGTAC CATGTATGTG CTGCTACGTC
CCTTTGAGAA TGTGCCATCG CTGTGGTGAG GCATGTGGTT GCTGTGGTGG GAAACATAAA
GCTGCTGGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011519591.1
CDS96..1367
Misc Feature(1)96..407(+)
Misc Feature(2)96..305(+)
Misc Feature(3)1026..1316(+)
Translation

Target ORF information:

RefSeq Version XM_011521289
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens sprouty-related, EVH1 domain containing 1 (SPRED1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011521289

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACCCGAG ATGACTCAAG TGGTGGATGG TTACCACTTG GAGGGAGTGG ACTAAGCAGC 
GTCACTGTCT TCAAAGTCCC TCATCAGGAA GAGAATGGCT GTGCTGACTT TTTTATCCGT
GGAGAGCGAC TCAGGGACAA AATGGTGGTT TTGGAATGTA TGCTTAAAAA AGACCTCATT
TATAATAAGG TCACTCCAAC ATTTCACCAC TGGAAGATTG ATGACAAGAA GTTTGGTCTT
ACGTTTCAAA GTCCTGCTGA TGCTAGGGCT TTTGATAGAG GTATCCGAAG AGCTATAGAG
GATATTTCTC AAGGATGCCC CGAATCAAAA AATGAAGCTG AAGGGGCAGA TGACTTACAA
GCAAATGAAG AGGATTCTTC CAGTTCTCTA GTGAAGGATC ACCTTTTTCA GCAAGAGACA
GTTGTTACCA GTGAGCCTTA TAGAAGCTCA AATATAAGAC CTTCTCCCTT TGAAGATCTG
AATGCCAGAA GAGTCTACAT GCAAAGCCAA GCCAATCAGA TAACATTTGG TCAGCCAGGC
TTGGACATTC AGAGCAGAAG TATGGAATAC GTACAGCGGC AAATATCCAA GGAATGTGGA
AGCCTAAAGT CCCAAAATAG GGTCCCTTTG AAATCAATCA GACATGTCAG CTTTCAAGAT
GAGGATGAGA TTGTCAGAAT AAACCCTCGA GATATCTTAA TACGTCGCTA TGCAGACTAC
AGACATCCTG ACATGTGGAA AAATGACTTG GAAAGAGATG ATGCTGATTC CAGTATTCAG
TTTTCTAAAC CAGACAGTAA AAAATCAGAC TATCTGTACT CTTGTGGGGA TGAGACTAAG
TTAAGTTCAC CCAAAGACTC TGTGGTATTT AAGACGCAGC CTTCCTCATT AAAAATTAAG
AAGTCAAAAC GAAGAAAAGA GGATGGTGAA CGTTCTCGCT GCGTATACTG CCAGGAAAGG
TTTAATCATG AAGAAAATGT TAGGGGAAAA TGTCAGGATG CTCCAGACCC TATTAAAAGA
TGCATATATC AAGTTAGTTG CATGCTCTGT GCAGAGAGCA TGTTGTATCA TTGTATGTCA
GACTCAGAGG GAGATTTTTC TGATCCCTGT TCGTGTGACA CTAGCGACGA CAAGTTCTGC
TTGCGATGGT TAGCCCTGGT AGCTTTGTCT TTCATTGTAC CATGTATGTG CTGCTACGTC
CCTTTGAGAA TGTGCCATCG CTGTGGTGAG GCATGTGGTT GCTGTGGTGG GAAACATAAA
GCTGCTGGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu48144
Clone ID Related Accession (Same CDS sequence) XM_005254203
Accession Version XM_005254203.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1113bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product sprouty-related, EVH1 domain-containing protein 1 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578826670. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCTTAAAA AAGACCTCAT TTATAATAAG GTCACTCCAA CATTTCACCA CTGGAAGATT 
GATGACAAGA AGTTTGGTCT TACGTTTCAA AGTCCTGCTG ATGCTAGGGC TTTTGATAGA
GGTATCCGAA GAGCTATAGA GGATATTTCT CAAGGATGCC CCGAATCAAA AAATGAAGCT
GAAGGGGCAG ATGACTTACA AGCAAATGAA GAGGATTCTT CCAGTTCTCT AGTGAAGGAT
CACCTTTTTC AGCAAGAGAC AGTTGTTACC AGTGAGCCTT ATAGAAGCTC AAATATAAGA
CCTTCTCCCT TTGAAGATCT GAATGCCAGA AGAGTCTACA TGCAAAGCCA AGCCAATCAG
ATAACATTTG GTCAGCCAGG CTTGGACATT CAGAGCAGAA GTATGGAATA CGTACAGCGG
CAAATATCCA AGGAATGTGG AAGCCTAAAG TCCCAAAATA GGGTCCCTTT GAAATCAATC
AGACATGTCA GCTTTCAAGA TGAGGATGAG ATTGTCAGAA TAAACCCTCG AGATATCTTA
ATACGTCGCT ATGCAGACTA CAGACATCCT GACATGTGGA AAAATGACTT GGAAAGAGAT
GATGCTGATT CCAGTATTCA GTTTTCTAAA CCAGACAGTA AAAAATCAGA CTATCTGTAC
TCTTGTGGGG ATGAGACTAA GTTAAGTTCA CCCAAAGACT CTGTGGTATT TAAGACGCAG
CCTTCCTCAT TAAAAATTAA GAAGTCAAAA CGAAGAAAAG AGGATGGTGA ACGTTCTCGC
TGCGTATACT GCCAGGAAAG GTTTAATCAT GAAGAAAATG TTAGGGGAAA ATGTCAGGAT
GCTCCAGACC CTATTAAAAG ATGCATATAT CAAGTTAGTT GCATGCTCTG TGCAGAGAGC
ATGTTGTATC ATTGTATGTC AGACTCAGAG GGAGATTTTT CTGATCCCTG TTCGTGTGAC
ACTAGCGACG ACAAGTTCTG CTTGCGATGG TTAGCCCTGG TAGCTTTGTC TTTCATTGTA
CCATGTATGT GCTGCTACGT CCCTTTGAGA ATGTGCCATC GCTGTGGTGA GGCATGTGGT
TGCTGTGGTG GGAAACATAA AGCTGCTGGA TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254260.1
CDS1147..2259
Misc Feature(1)1150..1299(+)
Misc Feature(2)1918..2208(+)
Translation

Target ORF information:

RefSeq Version XM_005254203
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens sprouty-related, EVH1 domain containing 1 (SPRED1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254203

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCTTAAAA AAGACCTCAT TTATAATAAG GTCACTCCAA CATTTCACCA CTGGAAGATT 
GATGACAAGA AGTTTGGTCT TACGTTTCAA AGTCCTGCTG ATGCTAGGGC TTTTGATAGA
GGTATCCGAA GAGCTATAGA GGATATTTCT CAAGGATGCC CCGAATCAAA AAATGAAGCT
GAAGGGGCAG ATGACTTACA AGCAAATGAA GAGGATTCTT CCAGTTCTCT AGTGAAGGAT
CACCTTTTTC AGCAAGAGAC AGTTGTTACC AGTGAGCCTT ATAGAAGCTC AAATATAAGA
CCTTCTCCCT TTGAAGATCT GAATGCCAGA AGAGTCTACA TGCAAAGCCA AGCCAATCAG
ATAACATTTG GTCAGCCAGG CTTGGACATT CAGAGCAGAA GTATGGAATA CGTACAGCGG
CAAATATCCA AGGAATGTGG AAGCCTAAAG TCCCAAAATA GGGTCCCTTT GAAATCAATC
AGACATGTCA GCTTTCAAGA TGAGGATGAG ATTGTCAGAA TAAACCCTCG AGATATCTTA
ATACGTCGCT ATGCAGACTA CAGACATCCT GACATGTGGA AAAATGACTT GGAAAGAGAT
GATGCTGATT CCAGTATTCA GTTTTCTAAA CCAGACAGTA AAAAATCAGA CTATCTGTAC
TCTTGTGGGG ATGAGACTAA GTTAAGTTCA CCCAAAGACT CTGTGGTATT TAAGACGCAG
CCTTCCTCAT TAAAAATTAA GAAGTCAAAA CGAAGAAAAG AGGATGGTGA ACGTTCTCGC
TGCGTATACT GCCAGGAAAG GTTTAATCAT GAAGAAAATG TTAGGGGAAA ATGTCAGGAT
GCTCCAGACC CTATTAAAAG ATGCATATAT CAAGTTAGTT GCATGCTCTG TGCAGAGAGC
ATGTTGTATC ATTGTATGTC AGACTCAGAG GGAGATTTTT CTGATCCCTG TTCGTGTGAC
ACTAGCGACG ACAAGTTCTG CTTGCGATGG TTAGCCCTGG TAGCTTTGTC TTTCATTGTA
CCATGTATGT GCTGCTACGT CCCTTTGAGA ATGTGCCATC GCTGTGGTGA GGCATGTGGT
TGCTGTGGTG GGAAACATAA AGCTGCTGGA TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu77141
Clone ID Related Accession (Same CDS sequence) XM_011521288 , XM_011521289 , XM_011521290
Accession Version XM_011521290.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1272bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product sprouty-related, EVH1 domain-containing protein 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACCCGAG ATGACTCAAG TGGTGGATGG TTACCACTTG GAGGGAGTGG ACTAAGCAGC 
GTCACTGTCT TCAAAGTCCC TCATCAGGAA GAGAATGGCT GTGCTGACTT TTTTATCCGT
GGAGAGCGAC TCAGGGACAA AATGGTGGTT TTGGAATGTA TGCTTAAAAA AGACCTCATT
TATAATAAGG TCACTCCAAC ATTTCACCAC TGGAAGATTG ATGACAAGAA GTTTGGTCTT
ACGTTTCAAA GTCCTGCTGA TGCTAGGGCT TTTGATAGAG GTATCCGAAG AGCTATAGAG
GATATTTCTC AAGGATGCCC CGAATCAAAA AATGAAGCTG AAGGGGCAGA TGACTTACAA
GCAAATGAAG AGGATTCTTC CAGTTCTCTA GTGAAGGATC ACCTTTTTCA GCAAGAGACA
GTTGTTACCA GTGAGCCTTA TAGAAGCTCA AATATAAGAC CTTCTCCCTT TGAAGATCTG
AATGCCAGAA GAGTCTACAT GCAAAGCCAA GCCAATCAGA TAACATTTGG TCAGCCAGGC
TTGGACATTC AGAGCAGAAG TATGGAATAC GTACAGCGGC AAATATCCAA GGAATGTGGA
AGCCTAAAGT CCCAAAATAG GGTCCCTTTG AAATCAATCA GACATGTCAG CTTTCAAGAT
GAGGATGAGA TTGTCAGAAT AAACCCTCGA GATATCTTAA TACGTCGCTA TGCAGACTAC
AGACATCCTG ACATGTGGAA AAATGACTTG GAAAGAGATG ATGCTGATTC CAGTATTCAG
TTTTCTAAAC CAGACAGTAA AAAATCAGAC TATCTGTACT CTTGTGGGGA TGAGACTAAG
TTAAGTTCAC CCAAAGACTC TGTGGTATTT AAGACGCAGC CTTCCTCATT AAAAATTAAG
AAGTCAAAAC GAAGAAAAGA GGATGGTGAA CGTTCTCGCT GCGTATACTG CCAGGAAAGG
TTTAATCATG AAGAAAATGT TAGGGGAAAA TGTCAGGATG CTCCAGACCC TATTAAAAGA
TGCATATATC AAGTTAGTTG CATGCTCTGT GCAGAGAGCA TGTTGTATCA TTGTATGTCA
GACTCAGAGG GAGATTTTTC TGATCCCTGT TCGTGTGACA CTAGCGACGA CAAGTTCTGC
TTGCGATGGT TAGCCCTGGT AGCTTTGTCT TTCATTGTAC CATGTATGTG CTGCTACGTC
CCTTTGAGAA TGTGCCATCG CTGTGGTGAG GCATGTGGTT GCTGTGGTGG GAAACATAAA
GCTGCTGGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011519592.1
CDS119..1390
Misc Feature(1)119..430(+)
Misc Feature(2)119..328(+)
Misc Feature(3)1049..1339(+)
Translation

Target ORF information:

RefSeq Version XM_011521290
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens sprouty-related, EVH1 domain containing 1 (SPRED1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011521290

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACCCGAG ATGACTCAAG TGGTGGATGG TTACCACTTG GAGGGAGTGG ACTAAGCAGC 
GTCACTGTCT TCAAAGTCCC TCATCAGGAA GAGAATGGCT GTGCTGACTT TTTTATCCGT
GGAGAGCGAC TCAGGGACAA AATGGTGGTT TTGGAATGTA TGCTTAAAAA AGACCTCATT
TATAATAAGG TCACTCCAAC ATTTCACCAC TGGAAGATTG ATGACAAGAA GTTTGGTCTT
ACGTTTCAAA GTCCTGCTGA TGCTAGGGCT TTTGATAGAG GTATCCGAAG AGCTATAGAG
GATATTTCTC AAGGATGCCC CGAATCAAAA AATGAAGCTG AAGGGGCAGA TGACTTACAA
GCAAATGAAG AGGATTCTTC CAGTTCTCTA GTGAAGGATC ACCTTTTTCA GCAAGAGACA
GTTGTTACCA GTGAGCCTTA TAGAAGCTCA AATATAAGAC CTTCTCCCTT TGAAGATCTG
AATGCCAGAA GAGTCTACAT GCAAAGCCAA GCCAATCAGA TAACATTTGG TCAGCCAGGC
TTGGACATTC AGAGCAGAAG TATGGAATAC GTACAGCGGC AAATATCCAA GGAATGTGGA
AGCCTAAAGT CCCAAAATAG GGTCCCTTTG AAATCAATCA GACATGTCAG CTTTCAAGAT
GAGGATGAGA TTGTCAGAAT AAACCCTCGA GATATCTTAA TACGTCGCTA TGCAGACTAC
AGACATCCTG ACATGTGGAA AAATGACTTG GAAAGAGATG ATGCTGATTC CAGTATTCAG
TTTTCTAAAC CAGACAGTAA AAAATCAGAC TATCTGTACT CTTGTGGGGA TGAGACTAAG
TTAAGTTCAC CCAAAGACTC TGTGGTATTT AAGACGCAGC CTTCCTCATT AAAAATTAAG
AAGTCAAAAC GAAGAAAAGA GGATGGTGAA CGTTCTCGCT GCGTATACTG CCAGGAAAGG
TTTAATCATG AAGAAAATGT TAGGGGAAAA TGTCAGGATG CTCCAGACCC TATTAAAAGA
TGCATATATC AAGTTAGTTG CATGCTCTGT GCAGAGAGCA TGTTGTATCA TTGTATGTCA
GACTCAGAGG GAGATTTTTC TGATCCCTGT TCGTGTGACA CTAGCGACGA CAAGTTCTGC
TTGCGATGGT TAGCCCTGGT AGCTTTGTCT TTCATTGTAC CATGTATGTG CTGCTACGTC
CCTTTGAGAA TGTGCCATCG CTGTGGTGAG GCATGTGGTT GCTGTGGTGG GAAACATAAA
GCTGCTGGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18529
Clone ID Related Accession (Same CDS sequence) NM_152594
Accession Version NM_152594.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1335bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product sprouty-related, EVH1 domain-containing protein 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA405650.1, AY299089.1 and AC069381.8. This sequence is a reference standard in the RefSeqGene project. On May 1, 2008 this sequence version replaced gi:22749220. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY299089.1, AY299089.1, BC137480.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGCGAGG AGACGGCGAC TTCTGACAAC GATAATAGTT ATGCACGAGT GCGAGCTGTG 
GTGATGACCC GAGATGACTC AAGTGGTGGA TGGTTACCAC TTGGAGGGAG TGGACTAAGC
AGCGTCACTG TCTTCAAAGT CCCTCATCAG GAAGAGAATG GCTGTGCTGA CTTTTTTATC
CGTGGAGAGC GACTCAGGGA CAAAATGGTG GTTTTGGAAT GTATGCTTAA AAAAGACCTC
ATTTATAATA AGGTCACTCC AACATTTCAC CACTGGAAGA TTGATGACAA GAAGTTTGGT
CTTACGTTTC AAAGTCCTGC TGATGCTAGG GCTTTTGATA GAGGTATCCG AAGAGCTATA
GAGGATATTT CTCAAGGATG CCCCGAATCA AAAAATGAAG CTGAAGGGGC AGATGACTTA
CAAGCAAATG AAGAGGATTC TTCCAGTTCT CTAGTGAAGG ATCACCTTTT TCAGCAAGAG
ACAGTTGTTA CCAGTGAGCC TTATAGAAGC TCAAATATAA GACCTTCTCC CTTTGAAGAT
CTGAATGCCA GAAGAGTCTA CATGCAAAGC CAAGCCAATC AGATAACATT TGGTCAGCCA
GGCTTGGACA TTCAGAGCAG AAGTATGGAA TACGTACAGC GGCAAATATC CAAGGAATGT
GGAAGCCTAA AGTCCCAAAA TAGGGTCCCT TTGAAATCAA TCAGACATGT CAGCTTTCAA
GATGAGGATG AGATTGTCAG AATAAACCCT CGAGATATCT TAATACGTCG CTATGCAGAC
TACAGACATC CTGACATGTG GAAAAATGAC TTGGAAAGAG ATGATGCTGA TTCCAGTATT
CAGTTTTCTA AACCAGACAG TAAAAAATCA GACTATCTGT ACTCTTGTGG GGATGAGACT
AAGTTAAGTT CACCCAAAGA CTCTGTGGTA TTTAAGACGC AGCCTTCCTC ATTAAAAATT
AAGAAGTCAA AACGAAGAAA AGAGGATGGT GAACGTTCTC GCTGCGTATA CTGCCAGGAA
AGGTTTAATC ATGAAGAAAA TGTTAGGGGA AAATGTCAGG ATGCTCCAGA CCCTATTAAA
AGATGCATAT ATCAAGTTAG TTGCATGCTC TGTGCAGAGA GCATGTTGTA TCATTGTATG
TCAGACTCAG AGGGAGATTT TTCTGATCCC TGTTCGTGTG ACACTAGCGA CGACAAGTTC
TGCTTGCGAT GGTTAGCCCT GGTAGCTTTG TCTTTCATTG TACCATGTAT GTGCTGCTAC
GTCCCTTTGA GAATGTGCCA TCGCTGTGGT GAGGCATGTG GTTGCTGTGG TGGGAAACAT
AAAGCTGCTG GATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_689807.1
CDS336..1670
Misc Feature(1)210..212(+)
Misc Feature(2)339..341(+)
Misc Feature(3)372..710(+)
Misc Feature(4)399..608(+)
Misc Feature(5)1329..1619(+)
Exon (1)1..367
Gene:SPRED1
Gene Synonym:
Exon (2)368..542
Gene:SPRED1
Gene Synonym:
Exon (3)543..711
Gene:SPRED1
Gene Synonym:
Exon (4)712..758
Gene:SPRED1
Gene Synonym:
Exon (5)759..917
Gene:SPRED1
Gene Synonym:
Exon (6)918..1019
Gene:SPRED1
Gene Synonym:
Exon (7)1020..7255
Gene:SPRED1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_152594
Organism Homo sapiens (human)
Definition Homo sapiens sprouty-related, EVH1 domain containing 1 (SPRED1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_152594

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGCGAGG AGACGGCGAC TTCTGACAAC GATAATAGTT ATGCACGAGT GCGAGCTGTG 
GTGATGACCC GAGATGACTC AAGTGGTGGA TGGTTACCAC TTGGAGGGAG TGGACTAAGC
AGCGTCACTG TCTTCAAAGT CCCTCATCAG GAAGAGAATG GCTGTGCTGA CTTTTTTATC
CGTGGAGAGC GACTCAGGGA CAAAATGGTG GTTTTGGAAT GTATGCTTAA AAAAGACCTC
ATTTATAATA AGGTCACTCC AACATTTCAC CACTGGAAGA TTGATGACAA GAAGTTTGGT
CTTACGTTTC AAAGTCCTGC TGATGCTAGG GCTTTTGATA GAGGTATCCG AAGAGCTATA
GAGGATATTT CTCAAGGATG CCCCGAATCA AAAAATGAAG CTGAAGGGGC AGATGACTTA
CAAGCAAATG AAGAGGATTC TTCCAGTTCT CTAGTGAAGG ATCACCTTTT TCAGCAAGAG
ACAGTTGTTA CCAGTGAGCC TTATAGAAGC TCAAATATAA GACCTTCTCC CTTTGAAGAT
CTGAATGCCA GAAGAGTCTA CATGCAAAGC CAAGCCAATC AGATAACATT TGGTCAGCCA
GGCTTGGACA TTCAGAGCAG AAGTATGGAA TACGTACAGC GGCAAATATC CAAGGAATGT
GGAAGCCTAA AGTCCCAAAA TAGGGTCCCT TTGAAATCAA TCAGACATGT CAGCTTTCAA
GATGAGGATG AGATTGTCAG AATAAACCCT CGAGATATCT TAATACGTCG CTATGCAGAC
TACAGACATC CTGACATGTG GAAAAATGAC TTGGAAAGAG ATGATGCTGA TTCCAGTATT
CAGTTTTCTA AACCAGACAG TAAAAAATCA GACTATCTGT ACTCTTGTGG GGATGAGACT
AAGTTAAGTT CACCCAAAGA CTCTGTGGTA TTTAAGACGC AGCCTTCCTC ATTAAAAATT
AAGAAGTCAA AACGAAGAAA AGAGGATGGT GAACGTTCTC GCTGCGTATA CTGCCAGGAA
AGGTTTAATC ATGAAGAAAA TGTTAGGGGA AAATGTCAGG ATGCTCCAGA CCCTATTAAA
AGATGCATAT ATCAAGTTAG TTGCATGCTC TGTGCAGAGA GCATGTTGTA TCATTGTATG
TCAGACTCAG AGGGAGATTT TTCTGATCCC TGTTCGTGTG ACACTAGCGA CGACAAGTTC
TGCTTGCGAT GGTTAGCCCT GGTAGCTTTG TCTTTCATTG TACCATGTAT GTGCTGCTAC
GTCCCTTTGA GAATGTGCCA TCGCTGTGGT GAGGCATGTG GTTGCTGTGG TGGGAAACAT
AAAGCTGCTG GATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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