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SPRED1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol SPRED1
Entrez Gene ID 161742
Full Name sprouty related EVH1 domain containing 1
Synonyms NFLS,PPP1R147,hSpred1,spred-1
General protein information
Preferred Names

sprouty related EVH1 domain containing 1

Names

sprouty-related, EVH1 domain-containing protein 1
protein phosphatase 1, regulatory subunit 147
suppressor of Ras/MAPK activation

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

15

15q14

Disorder MIM:

609291

Disorder Html: Legius syndrome, 611431 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_011521289.2 XP_011519591.1 sprouty-related, EVH1 domain-containing protein 1 isoform X2
XM_005254202.3 XP_005254259.1 sprouty-related, EVH1 domain-containing protein 1 isoform X1
NM_152594.2 NP_689807.1 sprouty-related, EVH1 domain-containing protein 1



Canis lupus familiaris (dog) SPRED1 XP_544609.3
Rattus norvegicus (Norway rat) Spred1 NP_001040554.1
Xenopus tropicalis (tropical clawed frog) spred1 NP_001008037.1
Gallus gallus (chicken) SPRED1 NP_001186638.1
Pan troglodytes (chimpanzee) SPRED1 XP_001138171.1
Bos taurus (cattle) SPRED1 NP_001179445.1
Mus musculus (house mouse) Spred1 NP_277059.1
Danio rerio (zebrafish) spred1 NP_998397.1
Homo sapiens (human) SPRED1 NP_689807.1


Related articles in PubMed

Interaction between a Domain of the Negative Regulator of the Ras-ERK Pathway, SPRED1 Protein, and the GTPase-activating Protein-related Domain of Neurofibromin Is Implicated in Legius Syndrome and Neurofibromatosis Type 1.
Hirata Y, Brems H, Suzuki M, Kanamori M, Okada M, Morita R, Llano-Rivas I, Ose T, Messiaen L, Legius E, Yoshimura A
The Journal of biological chemistry291(7)3124-34(2016 Feb)

Family with Legius syndrome (neurofibromatosis type 1-like syndrome).
Sakai N, Maeda T, Kawakami H, Uchiyama M, Harada K, Tsuboi R, Mitsuhashi Y
The Journal of dermatology42(7)703-5(2015 Jul)

Cosuppression of Sprouty and Sprouty-related negative regulators of FGF signalling in prostate cancer: a working hypothesis.
Assinder SJ, Beniamen D, Lovicu FJ
BioMed research international2015827462(2015)

MicroRNAs 206 and 21 cooperate to promote RAS-extracellular signal-regulated kinase signaling by suppressing the translation of RASA1 and SPRED1.
Sharma SB, Lin CC, Farrugia MK, McLaughlin SL, Ellis EJ, Brundage KM, Salkeni MA, Ruppert JM
Molecular and cellular biology34(22)4143-64(2014 Nov)

The palmitoyl acyltransferase HIP14 shares a high proportion of interactors with huntingtin: implications for a role in the pathogenesis of Huntington's disease.
Butland SL, Sanders SS, Schmidt ME, Riechers SP, Lin DT, Martin DD, Vaid K, Graham RK, Singaraja RR, Wanker EE, Conibear E, Hayden MR
Human molecular genetics23(15)4142-60(2014 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In one case we identified a nonsense mutation c.46C>T (p.Arg16*) in exon 2 of SPRED1 gene, confirming diagnosis of Legius syndrome. This mutation was reported previously.
Title: The first Slovak Legius syndrome patient carrying the SPRED1 gene mutation.

Results provide genetic evidence that miR-126, through its target gene Spred-1, plays a critical role in the development of retinal vascular layers.
Title: Strand and Cell Type-specific Function of microRNA-126 in Angiogenesis.

The EVH1 domain of Spred1 binds to the noncatalytic portion of the GAP-related domain of neurofibromin.
Title: The neurofibromin recruitment factor Spred1 binds to the GAP related domain without affecting Ras inactivation.

PURA may be a potential target of miR-144 and observed downregulation of PURA may be caused by increased expression of miR-144. The other predicted target of miR-144 SPRED1, was found to be downregulated in 69 per cent EC tissues as compared to matched distant non-malignant tissues.
Title: Potential diagnostic implications of miR-144 overexpression in human oesophageal cancer.

Data suggest SPRED1 EVH1 domain interacts with NF1 GRD domain [N-term. 16AA/C-term. 20AA of GTPase-activating protein-related domain]; SPRED1 EVH1 and NF1 GRD mutations observed in Legius syndrome reduce binding affinity between EVH1/GRD domains.
Title: Interaction between a Domain of the Negative Regulator of the Ras-ERK Pathway, SPRED1 Protein, and the GTPase-activating Protein-related Domain of Neurofibromin Is Implicated in Legius Syndrome and Neurofibromatosis Type 1.

The following SPRED1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPRED1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu18529 NM_152594.2
Latest version!
Homo sapiens sprouty related EVH1 domain containing 1 (SPRED1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu48143 XM_005254202.3
Latest version!
Homo sapiens sprouty related EVH1 domain containing 1 (SPRED1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu77141 XM_011521289.2
Latest version!
Homo sapiens sprouty related EVH1 domain containing 1 (SPRED1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18529
Clone ID Related Accession (Same CDS sequence) NM_152594.2
Accession Version NM_152594.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1335bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-09
Organism Homo sapiens(Human)
Product sprouty-related, EVH1 domain-containing protein 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA405650.1, AY299089.1 and AC069381.8. This sequence is a reference standard in the RefSeqGene project. On May 1, 2008 this sequence version replaced NM_152594.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.119512.1, SRR1660809.82757.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGCGAGG AGACGGCGAC TTCTGACAAC GATAATAGTT ATGCACGAGT GCGAGCTGTG 
GTGATGACCC GAGATGACTC AAGTGGTGGA TGGTTACCAC TTGGAGGGAG TGGACTAAGC
AGCGTCACTG TCTTCAAAGT CCCTCATCAG GAAGAGAATG GCTGTGCTGA CTTTTTTATC
CGTGGAGAGC GACTCAGGGA CAAAATGGTG GTTTTGGAAT GTATGCTTAA AAAAGACCTC
ATTTATAATA AGGTCACTCC AACATTTCAC CACTGGAAGA TTGATGACAA GAAGTTTGGT
CTTACGTTTC AAAGTCCTGC TGATGCTAGG GCTTTTGATA GAGGTATCCG AAGAGCTATA
GAGGATATTT CTCAAGGATG CCCCGAATCA AAAAATGAAG CTGAAGGGGC AGATGACTTA
CAAGCAAATG AAGAGGATTC TTCCAGTTCT CTAGTGAAGG ATCACCTTTT TCAGCAAGAG
ACAGTTGTTA CCAGTGAGCC TTATAGAAGC TCAAATATAA GACCTTCTCC CTTTGAAGAT
CTGAATGCCA GAAGAGTCTA CATGCAAAGC CAAGCCAATC AGATAACATT TGGTCAGCCA
GGCTTGGACA TTCAGAGCAG AAGTATGGAA TACGTACAGC GGCAAATATC CAAGGAATGT
GGAAGCCTAA AGTCCCAAAA TAGGGTCCCT TTGAAATCAA TCAGACATGT CAGCTTTCAA
GATGAGGATG AGATTGTCAG AATAAACCCT CGAGATATCT TAATACGTCG CTATGCAGAC
TACAGACATC CTGACATGTG GAAAAATGAC TTGGAAAGAG ATGATGCTGA TTCCAGTATT
CAGTTTTCTA AACCAGACAG TAAAAAATCA GACTATCTGT ACTCTTGTGG GGATGAGACT
AAGTTAAGTT CACCCAAAGA CTCTGTGGTA TTTAAGACGC AGCCTTCCTC ATTAAAAATT
AAGAAGTCAA AACGAAGAAA AGAGGATGGT GAACGTTCTC GCTGCGTATA CTGCCAGGAA
AGGTTTAATC ATGAAGAAAA TGTTAGGGGA AAATGTCAGG ATGCTCCAGA CCCTATTAAA
AGATGCATAT ATCAAGTTAG TTGCATGCTC TGTGCAGAGA GCATGTTGTA TCATTGTATG
TCAGACTCAG AGGGAGATTT TTCTGATCCC TGTTCGTGTG ACACTAGCGA CGACAAGTTC
TGCTTGCGAT GGTTAGCCCT GGTAGCTTTG TCTTTCATTG TACCATGTAT GTGCTGCTAC
GTCCCTTTGA GAATGTGCCA TCGCTGTGGT GAGGCATGTG GTTGCTGTGG TGGGAAACAT
AAAGCTGCTG GATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_689807.1
CDS336..1670
Misc Feature(1)210..212
Misc Feature(2)339..341
Misc Feature(3)372..710
Misc Feature(4)order(399..401,426..428,600..602,606..608)
Misc Feature(5)1005..1007
Misc Feature(6)1047..1049
Misc Feature(7)1257..1259
Misc Feature(8)1332..1619
Exon (1)1..367
Exon (2)368..542
Exon (3)543..711
Exon (4)712..758
Exon (5)759..917
Exon (6)918..1019
Exon (7)1020..7255
Translation

Target ORF information:

RefSeq Version NM_152594.2
Organism Homo sapiens(Human)
Definition Homo sapiens sprouty related EVH1 domain containing 1 (SPRED1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_152594.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGCGAGG AGACGGCGAC TTCTGACAAC GATAATAGTT ATGCACGAGT GCGAGCTGTG 
GTGATGACCC GAGATGACTC AAGTGGTGGA TGGTTACCAC TTGGAGGGAG TGGACTAAGC
AGCGTCACTG TCTTCAAAGT CCCTCATCAG GAAGAGAATG GCTGTGCTGA CTTTTTTATC
CGTGGAGAGC GACTCAGGGA CAAAATGGTG GTTTTGGAAT GTATGCTTAA AAAAGACCTC
ATTTATAATA AGGTCACTCC AACATTTCAC CACTGGAAGA TTGATGACAA GAAGTTTGGT
CTTACGTTTC AAAGTCCTGC TGATGCTAGG GCTTTTGATA GAGGTATCCG AAGAGCTATA
GAGGATATTT CTCAAGGATG CCCCGAATCA AAAAATGAAG CTGAAGGGGC AGATGACTTA
CAAGCAAATG AAGAGGATTC TTCCAGTTCT CTAGTGAAGG ATCACCTTTT TCAGCAAGAG
ACAGTTGTTA CCAGTGAGCC TTATAGAAGC TCAAATATAA GACCTTCTCC CTTTGAAGAT
CTGAATGCCA GAAGAGTCTA CATGCAAAGC CAAGCCAATC AGATAACATT TGGTCAGCCA
GGCTTGGACA TTCAGAGCAG AAGTATGGAA TACGTACAGC GGCAAATATC CAAGGAATGT
GGAAGCCTAA AGTCCCAAAA TAGGGTCCCT TTGAAATCAA TCAGACATGT CAGCTTTCAA
GATGAGGATG AGATTGTCAG AATAAACCCT CGAGATATCT TAATACGTCG CTATGCAGAC
TACAGACATC CTGACATGTG GAAAAATGAC TTGGAAAGAG ATGATGCTGA TTCCAGTATT
CAGTTTTCTA AACCAGACAG TAAAAAATCA GACTATCTGT ACTCTTGTGG GGATGAGACT
AAGTTAAGTT CACCCAAAGA CTCTGTGGTA TTTAAGACGC AGCCTTCCTC ATTAAAAATT
AAGAAGTCAA AACGAAGAAA AGAGGATGGT GAACGTTCTC GCTGCGTATA CTGCCAGGAA
AGGTTTAATC ATGAAGAAAA TGTTAGGGGA AAATGTCAGG ATGCTCCAGA CCCTATTAAA
AGATGCATAT ATCAAGTTAG TTGCATGCTC TGTGCAGAGA GCATGTTGTA TCATTGTATG
TCAGACTCAG AGGGAGATTT TTCTGATCCC TGTTCGTGTG ACACTAGCGA CGACAAGTTC
TGCTTGCGAT GGTTAGCCCT GGTAGCTTTG TCTTTCATTG TACCATGTAT GTGCTGCTAC
GTCCCTTTGA GAATGTGCCA TCGCTGTGGT GAGGCATGTG GTTGCTGTGG TGGGAAACAT
AAAGCTGCTG GATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu48143
Clone ID Related Accession (Same CDS sequence) XM_005254202.3
Accession Version XM_005254202.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1371bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product sprouty-related, EVH1 domain-containing protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005254202.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGCGAGG AGACGGCGAC TTCTGACAAC GAGTGTCAGG GGCATGATGA TGGAATCTGC 
TTTTTTCATA ATAGTTATGC ACGAGTGCGA GCTGTGGTGA TGACCCGAGA TGACTCAAGT
GGTGGATGGT TACCACTTGG AGGGAGTGGA CTAAGCAGCG TCACTGTCTT CAAAGTCCCT
CATCAGGAAG AGAATGGCTG TGCTGACTTT TTTATCCGTG GAGAGCGACT CAGGGACAAA
ATGGTGGTTT TGGAATGTAT GCTTAAAAAA GACCTCATTT ATAATAAGGT CACTCCAACA
TTTCACCACT GGAAGATTGA TGACAAGAAG TTTGGTCTTA CGTTTCAAAG TCCTGCTGAT
GCTAGGGCTT TTGATAGAGG TATCCGAAGA GCTATAGAGG ATATTTCTCA AGGATGCCCC
GAATCAAAAA ATGAAGCTGA AGGGGCAGAT GACTTACAAG CAAATGAAGA GGATTCTTCC
AGTTCTCTAG TGAAGGATCA CCTTTTTCAG CAAGAGACAG TTGTTACCAG TGAGCCTTAT
AGAAGCTCAA ATATAAGACC TTCTCCCTTT GAAGATCTGA ATGCCAGAAG AGTCTACATG
CAAAGCCAAG CCAATCAGAT AACATTTGGT CAGCCAGGCT TGGACATTCA GAGCAGAAGT
ATGGAATACG TACAGCGGCA AATATCCAAG GAATGTGGAA GCCTAAAGTC CCAAAATAGG
GTCCCTTTGA AATCAATCAG ACATGTCAGC TTTCAAGATG AGGATGAGAT TGTCAGAATA
AACCCTCGAG ATATCTTAAT ACGTCGCTAT GCAGACTACA GACATCCTGA CATGTGGAAA
AATGACTTGG AAAGAGATGA TGCTGATTCC AGTATTCAGT TTTCTAAACC AGACAGTAAA
AAATCAGACT ATCTGTACTC TTGTGGGGAT GAGACTAAGT TAAGTTCACC CAAAGACTCT
GTGGTATTTA AGACGCAGCC TTCCTCATTA AAAATTAAGA AGTCAAAACG AAGAAAAGAG
GATGGTGAAC GTTCTCGCTG CGTATACTGC CAGGAAAGGT TTAATCATGA AGAAAATGTT
AGGGGAAAAT GTCAGGATGC TCCAGACCCT ATTAAAAGAT GCATATATCA AGTTAGTTGC
ATGCTCTGTG CAGAGAGCAT GTTGTATCAT TGTATGTCAG ACTCAGAGGG AGATTTTTCT
GATCCCTGTT CGTGTGACAC TAGCGACGAC AAGTTCTGCT TGCGATGGTT AGCCCTGGTA
GCTTTGTCTT TCATTGTACC ATGTATGTGC TGCTACGTCC CTTTGAGAAT GTGCCATCGC
TGTGGTGAGG CATGTGGTTG CTGTGGTGGG AAACATAAAG CTGCTGGATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254259.1
CDS1100..2470
Misc Feature(1)1172..1510
Misc Feature(2)order(1199..1201,1226..1228,1400..1402,1406..1408)
Misc Feature(3)2132..2419
Translation

Target ORF information:

RefSeq Version XM_005254202.3
Organism Homo sapiens(Human)
Definition Homo sapiens sprouty related EVH1 domain containing 1 (SPRED1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254202.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGCGAGG AGACGGCGAC TTCTGACAAC GAGTGTCAGG GGCATGATGA TGGAATCTGC 
TTTTTTCATA ATAGTTATGC ACGAGTGCGA GCTGTGGTGA TGACCCGAGA TGACTCAAGT
GGTGGATGGT TACCACTTGG AGGGAGTGGA CTAAGCAGCG TCACTGTCTT CAAAGTCCCT
CATCAGGAAG AGAATGGCTG TGCTGACTTT TTTATCCGTG GAGAGCGACT CAGGGACAAA
ATGGTGGTTT TGGAATGTAT GCTTAAAAAA GACCTCATTT ATAATAAGGT CACTCCAACA
TTTCACCACT GGAAGATTGA TGACAAGAAG TTTGGTCTTA CGTTTCAAAG TCCTGCTGAT
GCTAGGGCTT TTGATAGAGG TATCCGAAGA GCTATAGAGG ATATTTCTCA AGGATGCCCC
GAATCAAAAA ATGAAGCTGA AGGGGCAGAT GACTTACAAG CAAATGAAGA GGATTCTTCC
AGTTCTCTAG TGAAGGATCA CCTTTTTCAG CAAGAGACAG TTGTTACCAG TGAGCCTTAT
AGAAGCTCAA ATATAAGACC TTCTCCCTTT GAAGATCTGA ATGCCAGAAG AGTCTACATG
CAAAGCCAAG CCAATCAGAT AACATTTGGT CAGCCAGGCT TGGACATTCA GAGCAGAAGT
ATGGAATACG TACAGCGGCA AATATCCAAG GAATGTGGAA GCCTAAAGTC CCAAAATAGG
GTCCCTTTGA AATCAATCAG ACATGTCAGC TTTCAAGATG AGGATGAGAT TGTCAGAATA
AACCCTCGAG ATATCTTAAT ACGTCGCTAT GCAGACTACA GACATCCTGA CATGTGGAAA
AATGACTTGG AAAGAGATGA TGCTGATTCC AGTATTCAGT TTTCTAAACC AGACAGTAAA
AAATCAGACT ATCTGTACTC TTGTGGGGAT GAGACTAAGT TAAGTTCACC CAAAGACTCT
GTGGTATTTA AGACGCAGCC TTCCTCATTA AAAATTAAGA AGTCAAAACG AAGAAAAGAG
GATGGTGAAC GTTCTCGCTG CGTATACTGC CAGGAAAGGT TTAATCATGA AGAAAATGTT
AGGGGAAAAT GTCAGGATGC TCCAGACCCT ATTAAAAGAT GCATATATCA AGTTAGTTGC
ATGCTCTGTG CAGAGAGCAT GTTGTATCAT TGTATGTCAG ACTCAGAGGG AGATTTTTCT
GATCCCTGTT CGTGTGACAC TAGCGACGAC AAGTTCTGCT TGCGATGGTT AGCCCTGGTA
GCTTTGTCTT TCATTGTACC ATGTATGTGC TGCTACGTCC CTTTGAGAAT GTGCCATCGC
TGTGGTGAGG CATGTGGTTG CTGTGGTGGG AAACATAAAG CTGCTGGATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu77141
Clone ID Related Accession (Same CDS sequence) XM_011521289.2
Accession Version XM_011521289.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1272bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product sprouty-related, EVH1 domain-containing protein 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521289.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACCCGAG ATGACTCAAG TGGTGGATGG TTACCACTTG GAGGGAGTGG ACTAAGCAGC 
GTCACTGTCT TCAAAGTCCC TCATCAGGAA GAGAATGGCT GTGCTGACTT TTTTATCCGT
GGAGAGCGAC TCAGGGACAA AATGGTGGTT TTGGAATGTA TGCTTAAAAA AGACCTCATT
TATAATAAGG TCACTCCAAC ATTTCACCAC TGGAAGATTG ATGACAAGAA GTTTGGTCTT
ACGTTTCAAA GTCCTGCTGA TGCTAGGGCT TTTGATAGAG GTATCCGAAG AGCTATAGAG
GATATTTCTC AAGGATGCCC CGAATCAAAA AATGAAGCTG AAGGGGCAGA TGACTTACAA
GCAAATGAAG AGGATTCTTC CAGTTCTCTA GTGAAGGATC ACCTTTTTCA GCAAGAGACA
GTTGTTACCA GTGAGCCTTA TAGAAGCTCA AATATAAGAC CTTCTCCCTT TGAAGATCTG
AATGCCAGAA GAGTCTACAT GCAAAGCCAA GCCAATCAGA TAACATTTGG TCAGCCAGGC
TTGGACATTC AGAGCAGAAG TATGGAATAC GTACAGCGGC AAATATCCAA GGAATGTGGA
AGCCTAAAGT CCCAAAATAG GGTCCCTTTG AAATCAATCA GACATGTCAG CTTTCAAGAT
GAGGATGAGA TTGTCAGAAT AAACCCTCGA GATATCTTAA TACGTCGCTA TGCAGACTAC
AGACATCCTG ACATGTGGAA AAATGACTTG GAAAGAGATG ATGCTGATTC CAGTATTCAG
TTTTCTAAAC CAGACAGTAA AAAATCAGAC TATCTGTACT CTTGTGGGGA TGAGACTAAG
TTAAGTTCAC CCAAAGACTC TGTGGTATTT AAGACGCAGC CTTCCTCATT AAAAATTAAG
AAGTCAAAAC GAAGAAAAGA GGATGGTGAA CGTTCTCGCT GCGTATACTG CCAGGAAAGG
TTTAATCATG AAGAAAATGT TAGGGGAAAA TGTCAGGATG CTCCAGACCC TATTAAAAGA
TGCATATATC AAGTTAGTTG CATGCTCTGT GCAGAGAGCA TGTTGTATCA TTGTATGTCA
GACTCAGAGG GAGATTTTTC TGATCCCTGT TCGTGTGACA CTAGCGACGA CAAGTTCTGC
TTGCGATGGT TAGCCCTGGT AGCTTTGTCT TTCATTGTAC CATGTATGTG CTGCTACGTC
CCTTTGAGAA TGTGCCATCG CTGTGGTGAG GCATGTGGTT GCTGTGGTGG GAAACATAAA
GCTGCTGGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011519591.1
CDS96..1367
Misc Feature(1)96..407
Misc Feature(2)order(96..98,123..125,297..299,303..305)
Misc Feature(3)1029..1316
Translation

Target ORF information:

RefSeq Version XM_011521289.2
Organism Homo sapiens(Human)
Definition Homo sapiens sprouty related EVH1 domain containing 1 (SPRED1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011521289.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACCCGAG ATGACTCAAG TGGTGGATGG TTACCACTTG GAGGGAGTGG ACTAAGCAGC 
GTCACTGTCT TCAAAGTCCC TCATCAGGAA GAGAATGGCT GTGCTGACTT TTTTATCCGT
GGAGAGCGAC TCAGGGACAA AATGGTGGTT TTGGAATGTA TGCTTAAAAA AGACCTCATT
TATAATAAGG TCACTCCAAC ATTTCACCAC TGGAAGATTG ATGACAAGAA GTTTGGTCTT
ACGTTTCAAA GTCCTGCTGA TGCTAGGGCT TTTGATAGAG GTATCCGAAG AGCTATAGAG
GATATTTCTC AAGGATGCCC CGAATCAAAA AATGAAGCTG AAGGGGCAGA TGACTTACAA
GCAAATGAAG AGGATTCTTC CAGTTCTCTA GTGAAGGATC ACCTTTTTCA GCAAGAGACA
GTTGTTACCA GTGAGCCTTA TAGAAGCTCA AATATAAGAC CTTCTCCCTT TGAAGATCTG
AATGCCAGAA GAGTCTACAT GCAAAGCCAA GCCAATCAGA TAACATTTGG TCAGCCAGGC
TTGGACATTC AGAGCAGAAG TATGGAATAC GTACAGCGGC AAATATCCAA GGAATGTGGA
AGCCTAAAGT CCCAAAATAG GGTCCCTTTG AAATCAATCA GACATGTCAG CTTTCAAGAT
GAGGATGAGA TTGTCAGAAT AAACCCTCGA GATATCTTAA TACGTCGCTA TGCAGACTAC
AGACATCCTG ACATGTGGAA AAATGACTTG GAAAGAGATG ATGCTGATTC CAGTATTCAG
TTTTCTAAAC CAGACAGTAA AAAATCAGAC TATCTGTACT CTTGTGGGGA TGAGACTAAG
TTAAGTTCAC CCAAAGACTC TGTGGTATTT AAGACGCAGC CTTCCTCATT AAAAATTAAG
AAGTCAAAAC GAAGAAAAGA GGATGGTGAA CGTTCTCGCT GCGTATACTG CCAGGAAAGG
TTTAATCATG AAGAAAATGT TAGGGGAAAA TGTCAGGATG CTCCAGACCC TATTAAAAGA
TGCATATATC AAGTTAGTTG CATGCTCTGT GCAGAGAGCA TGTTGTATCA TTGTATGTCA
GACTCAGAGG GAGATTTTTC TGATCCCTGT TCGTGTGACA CTAGCGACGA CAAGTTCTGC
TTGCGATGGT TAGCCCTGGT AGCTTTGTCT TTCATTGTAC CATGTATGTG CTGCTACGTC
CCTTTGAGAA TGTGCCATCG CTGTGGTGAG GCATGTGGTT GCTGTGGTGG GAAACATAAA
GCTGCTGGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Interaction between a Domain of the Negative Regulator of the Ras-ERK Pathway, SPRED1 Protein, and the GTPase-activating Protein-related Domain of Neurofibromin Is Implicated in Legius Syndrome and Neurofibromatosis Type 1.
The Journal of biological chemistry291(7)3124-34(2016 Feb)
Hirata Y,Brems H,Suzuki M,Kanamori M,Okada M,Morita R,Llano-Rivas I,Ose T,Messiaen L,Legius E,Yoshimura A


book

Family with Legius syndrome (neurofibromatosis type 1-like syndrome).
The Journal of dermatology42(7)703-5(2015 Jul)
Sakai N,Maeda T,Kawakami H,Uchiyama M,Harada K,Tsuboi R,Mitsuhashi Y


book

Cosuppression of Sprouty and Sprouty-related negative regulators of FGF signalling in prostate cancer: a working hypothesis.
BioMed research international2015827462(2015)
Assinder SJ,Beniamen D,Lovicu FJ


book

MicroRNAs 206 and 21 cooperate to promote RAS-extracellular signal-regulated kinase signaling by suppressing the translation of RASA1 and SPRED1.
Molecular and cellular biology34(22)4143-64(2014 Nov)
Sharma SB,Lin CC,Farrugia MK,McLaughlin SL,Ellis EJ,Brundage KM,Salkeni MA,Ruppert JM


book

The palmitoyl acyltransferase HIP14 shares a high proportion of interactors with huntingtin: implications for a role in the pathogenesis of Huntington's disease.
Human molecular genetics23(15)4142-60(2014 Aug)
Butland SL,Sanders SS,Schmidt ME,Riechers SP,Lin DT,Martin DD,Vaid K,Graham RK,Singaraja RR,Wanker EE,Conibear E,Hayden MR


 
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