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ACE cDNA ORF clone, Homo sapiens (human)

Gene Symbol ACE
Entrez Gene ID 1636
Full Name angiotensin I converting enzyme
Synonyms ACE1, CD143, DCP, DCP1, ICH, MVCD3
General protein information
Preferred Names
angiotensin-converting enzyme
Names
angiotensin-converting enzyme
kininase II
peptidase P
CD143 antigen
testicular ECA
carboxycathepsin
dipeptidyl carboxypeptidase 1
dipeptidyl carboxypeptidase I
angiotensin converting enzyme, somatic isoform
angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
angiotensin I converting enzyme peptidyl-dipeptidase A 1 transcript
NP_000780.1
NP_001171528.1
NP_690043.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17q23.3

Summary This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, and two most abundant spliced variants encode the somatic form and the testicular form, respectively, that are equally active. [provided by RefSeq, May 2010]. lac of sum
Disorder MIM:

106180

Disorder Html: {Myocardial infarction, susceptibility to} (3); {Alzheimer disease,

mRNA and Protein(s)

mRNA Protein Name
XM_005257110 XP_005257167 angiotensin-converting enzyme isoform X1
XM_006721737 XP_006721800 angiotensin-converting enzyme isoform X2
NM_000789 NP_000780 angiotensin-converting enzyme isoform 1 precursor
NM_152830 NP_690043 angiotensin-converting enzyme isoform 2 precursor
NM_001178057 NP_001171528 angiotensin-converting enzyme isoform 3 precursor



Homo sapiens (human) ACE NP_000780.1
Pan troglodytes (chimpanzee) ACE NP_001008995.1
Macaca mulatta (Rhesus monkey) LOC100428661 XP_002800616.1
Canis lupus familiaris (dog) LOC610668 XP_003639297.1
Bos taurus (cattle) ACE NP_001193597.1
Mus musculus (house mouse) Ace NP_997507.1
Rattus norvegicus (Norway rat) Ace NP_036676.1
Gallus gallus (chicken) ACE NP_001161204.1
Danio rerio (zebrafish) ace XP_694336.5
Xenopus (Silurana) tropicalis (western clawed frog) ace NP_001116882.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ACE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu50168 XM_005257110 PREDICTED: Homo sapiens angiotensin I converting enzyme (ACE), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $559.30
$799.00
OHu57207 XM_006721737 PREDICTED: Homo sapiens angiotensin I converting enzyme (ACE), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu20567 NM_000789 Homo sapiens angiotensin I converting enzyme (ACE), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $923.30
$1319.00
OHu14691 NM_152830 Homo sapiens angiotensin I converting enzyme (ACE), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $517.30
$739.00
OHu14342 NM_001178057 Homo sapiens angiotensin I converting enzyme (ACE), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $489.30
$699.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu50168
Clone ID Related Accession (Same CDS sequence) XM_005257110
Accession Version XM_005257110.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3372bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product angiotensin-converting enzyme isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGCTCCTGT TTGCCTGGGA GGGCTGGCAC AACGCTGCGG GCATCCCGCT GAAACCGCTG 
TACGAGGATT TCACTGCCCT CAGCAATGAA GCCTACAAGC AGGACGGCTT CACAGACACG
GGGGCCTACT GGCGCTCCTG GTACAACTCC CCCACCTTCG AGGACGATCT GGAACACCTC
TACCAACAGC TAGAGCCCCT CTACCTGAAC CTCCATGCCT TCGTCCGCCG CGCACTGCAT
CGCCGATACG GAGACAGATA CATCAACCTC AGGGGACCCA TCCCTGCTCA TCTGCTGGGA
GACATGTGGG CCCAGAGCTG GGAAAACATC TACGACATGG TGGTGCCTTT CCCAGACAAG
CCCAACCTCG ATGTCACCAG TACTATGCTG CAGCAGGGCT GGAACGCCAC GCACATGTTC
CGGGTGGCAG AGGAGTTCTT CACCTCCCTG GAGCTCTCCC CCATGCCTCC CGAGTTCTGG
GAAGGGTCGA TGCTGGAGAA GCCGGCCGAC GGGCGGGAAG TGGTGTGCCA CGCCTCGGCT
TGGGACTTCT ACAACAGGAA AGACTTCAGG ATCAAGCAGT GCACACGGGT CACGATGGAC
CAGCTCTCCA CAGTGCACCA TGAGATGGGC CATATACAGT ACTACCTGCA GTACAAGGAT
CTGCCCGTCT CCCTGCGTCG GGGGGCCAAC CCCGGCTTCC ATGAGGCCAT TGGGGACGTG
CTGGCGCTCT CGGTCTCCAC TCCTGAACAT CTGCACAAAA TCGGCCTGCT GGACCGTGTC
ACCAATGACA CGGAAAGTGA CATCAATTAC TTGCTAAAAA TGGCACTGGA AAAAATTGCC
TTCCTGCCCT TTGGCTACTT GGTGGACCAG TGGCGCTGGG GGGTCTTTAG TGGGCGTACC
CCCCCTTCCC GCTACAACTT CGACTGGTGG TATCTTCGAA CCAAGTATCA GGGGATCTGT
CCTCCTGTTA CCCGAAACGA AACCCACTTT GATGCTGGAG CTAAGTTTCA TGTTCCAAAT
GTGACACCAT ACATCAGGTA CTTTGTGAGT TTTGTCCTGC AGTTCCAGTT CCATGAAGCC
CTGTGCAAGG AGGCAGGCTA TGAGGGCCCA CTGCACCAGT GTGACATCTA CCGGTCCACC
AAGGCAGGGG CCAAGCTCCG GAAGGTGCTG CAGGCTGGCT CCTCCAGGCC CTGGCAGGAG
GTGCTGAAGG ACATGGTCGG CTTAGATGCC CTGGATGCCC AGCCGCTGCT CAAGTACTTC
CAGCCAGTCA CCCAGTGGCT GCAGGAGCAG AACCAGCAGA ACGGCGAGGT CCTGGGCTGG
CCCGAGTACC AGTGGCACCC GCCGTTGCCT GACAACTACC CGGAGGGCAT AGACCTGGTG
ACTGATGAGG CTGAGGCCAG CAAGTTTGTG GAGGAATATG ACCGGACATC CCAGGTGGTG
TGGAACGAGT ATGCCGAGGC CAACTGGAAC TACAACACCA ACATCACCAC AGAGACCAGC
AAGATTCTGC TGCAGAAGAA CATGCAAATA GCCAACCACA CCCTGAAGTA CGGCACCCAG
GCCAGGAAGT TTGATGTGAA CCAGTTGCAG AACACCACTA TCAAGCGGAT CATAAAGAAG
GTTCAGGACC TAGAACGGGC AGCACTGCCT GCCCAGGAGC TGGAGGAGTA CAACAAGATC
CTGTTGGATA TGGAAACCAC CTACAGCGTG GCCACTGTGT GCCACCCGAA TGGCAGCTGC
CTGCAGCTCG AGCCAGATCT GACGAATGTG ATGGCCACGT CCCGGAAATA TGAAGACCTG
TTATGGGCAT GGGAGGGCTG GCGAGACAAG GCGGGGAGAG CCATCCTCCA GTTTTACCCG
AAATACGTGG AACTCATCAA CCAGGCTGCC CGGCTCAATG GCTATGTAGA TGCAGGGGAC
TCGTGGAGGT CTATGTACGA GACACCATCC CTGGAGCAAG ACCTGGAGCG GCTCTTCCAG
GAGCTGCAGC CACTCTACCT CAACCTGCAT GCCTACGTGC GCCGGGCCCT GCACCGTCAC
TACGGGGCCC AGCACATCAA CCTGGAGGGG CCCATTCCTG CTCACCTGCT GGGGAACATG
TGGGCGCAGA CCTGGTCCAA CATCTATGAC TTGGTGGTGC CCTTCCCTTC AGCCCCCTCG
ATGGACACCA CAGAGGCTAT GCTAAAGCAG GGCTGGACGC CCAGGAGGAT GTTTAAGGAG
GCTGATGATT TCTTCACCTC CCTGGGGCTG CTGCCCGTGC CTCCTGAGTT CTGGAACAAG
TCGATGCTGG AGAAGCCAAC CGACGGGCGG GAGGTGGTCT GCCACGCCTC GGCCTGGGAC
TTCTACAACG GCAAGGACTT CCGGATCAAG CAGTGCACCA CCGTGAACTT GGAGGACCTG
GTGGTGGCCC ACCACGAAAT GGGCCACATC CAGTATTTCA TGCAGTACAA AGACTTACCT
GTGGCCTTGA GGGAGGGTGC CAACCCCGGC TTCCATGAGG CCATTGGGGA CGTGCTAGCC
CTCTCAGTGT CTACGCCCAA GCACCTGCAC AGTCTCAACC TGCTGAGCAG TGAGGGTGGC
AGCGACGAGC ATGACATCAA CTTTCTGATG AAGATGGCCC TTGACAAGAT CGCCTTTATC
CCCTTCAGCT ACCTCGTCGA TCAGTGGCGC TGGAGGGTAT TTGATGGAAG CATCACCAAG
GAGAACTATA ACCAGGAGTG GTGGAGCCTC AGGCTGAAGT ACCAGGGCCT CTGCCCCCCA
GTGCCCAGGA CTCAAGGTGA CTTTGACCCA GGGGCCAAGT TCCACATTCC TTCTAGCGTG
CCTTACATCA GGTACTTTGT CAGCTTCATC ATCCAGTTCC AGTTCCACGA GGCACTGTGC
CAGGCAGCTG GCCACACGGG CCCCCTGCAC AAGTGTGACA TCTACCAGTC CAAGGAGGCC
GGGCAGCGCC TGGCGACCGC CATGAAGCTG GGCTTCAGTA GGCCGTGGCC GGAAGCCATG
CAGCTGATCA CGGGCCAGCC CAACATGAGC GCCTCGGCCA TGTTGAGCTA CTTCAAGCCG
CTGCTGGACT GGCTCCGCAC GGAGAACGAG CTGCATGGGG AGAAGCTGGG CTGGCCGCAG
TACAACTGGA CGCCGAACTC CGCTCGCTCA GAAGGGCCCC TCCCAGACAG CGGCCGCGTC
AGCTTCCTGG GCCTGGACCT GGATGCGCAG CAGGCCCGCG TGGGCCAGTG GCTGCTGCTC
TTCCTGGGCA TCGCCCTGCT GGTAGCCACC CTGGGCCTCA GCCAGCGGCT CTTCAGCATC
CGCCACCGCA GCCTCCACCG GCACTCCCAC GGGCCCCAGT TCGGCTCCGA GGTGGAGCTG
AGACACTCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005257167.1
CDS490..3861
Misc Feature(1)493..1782(+)
Misc Feature(2)1018..1530(+)
Misc Feature(3)1108..1194(+)
Misc Feature(4)1894..3576(+)
Misc Feature(5)2812..3324(+)
Misc Feature(6)2902..2988(+)
Translation

Target ORF information:

RefSeq Version XM_005257110
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens angiotensin I converting enzyme (ACE), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257110

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGCTCCTGT TTGCCTGGGA GGGCTGGCAC AACGCTGCGG GCATCCCGCT GAAACCGCTG 
TACGAGGATT TCACTGCCCT CAGCAATGAA GCCTACAAGC AGGACGGCTT CACAGACACG
GGGGCCTACT GGCGCTCCTG GTACAACTCC CCCACCTTCG AGGACGATCT GGAACACCTC
TACCAACAGC TAGAGCCCCT CTACCTGAAC CTCCATGCCT TCGTCCGCCG CGCACTGCAT
CGCCGATACG GAGACAGATA CATCAACCTC AGGGGACCCA TCCCTGCTCA TCTGCTGGGA
GACATGTGGG CCCAGAGCTG GGAAAACATC TACGACATGG TGGTGCCTTT CCCAGACAAG
CCCAACCTCG ATGTCACCAG TACTATGCTG CAGCAGGGCT GGAACGCCAC GCACATGTTC
CGGGTGGCAG AGGAGTTCTT CACCTCCCTG GAGCTCTCCC CCATGCCTCC CGAGTTCTGG
GAAGGGTCGA TGCTGGAGAA GCCGGCCGAC GGGCGGGAAG TGGTGTGCCA CGCCTCGGCT
TGGGACTTCT ACAACAGGAA AGACTTCAGG ATCAAGCAGT GCACACGGGT CACGATGGAC
CAGCTCTCCA CAGTGCACCA TGAGATGGGC CATATACAGT ACTACCTGCA GTACAAGGAT
CTGCCCGTCT CCCTGCGTCG GGGGGCCAAC CCCGGCTTCC ATGAGGCCAT TGGGGACGTG
CTGGCGCTCT CGGTCTCCAC TCCTGAACAT CTGCACAAAA TCGGCCTGCT GGACCGTGTC
ACCAATGACA CGGAAAGTGA CATCAATTAC TTGCTAAAAA TGGCACTGGA AAAAATTGCC
TTCCTGCCCT TTGGCTACTT GGTGGACCAG TGGCGCTGGG GGGTCTTTAG TGGGCGTACC
CCCCCTTCCC GCTACAACTT CGACTGGTGG TATCTTCGAA CCAAGTATCA GGGGATCTGT
CCTCCTGTTA CCCGAAACGA AACCCACTTT GATGCTGGAG CTAAGTTTCA TGTTCCAAAT
GTGACACCAT ACATCAGGTA CTTTGTGAGT TTTGTCCTGC AGTTCCAGTT CCATGAAGCC
CTGTGCAAGG AGGCAGGCTA TGAGGGCCCA CTGCACCAGT GTGACATCTA CCGGTCCACC
AAGGCAGGGG CCAAGCTCCG GAAGGTGCTG CAGGCTGGCT CCTCCAGGCC CTGGCAGGAG
GTGCTGAAGG ACATGGTCGG CTTAGATGCC CTGGATGCCC AGCCGCTGCT CAAGTACTTC
CAGCCAGTCA CCCAGTGGCT GCAGGAGCAG AACCAGCAGA ACGGCGAGGT CCTGGGCTGG
CCCGAGTACC AGTGGCACCC GCCGTTGCCT GACAACTACC CGGAGGGCAT AGACCTGGTG
ACTGATGAGG CTGAGGCCAG CAAGTTTGTG GAGGAATATG ACCGGACATC CCAGGTGGTG
TGGAACGAGT ATGCCGAGGC CAACTGGAAC TACAACACCA ACATCACCAC AGAGACCAGC
AAGATTCTGC TGCAGAAGAA CATGCAAATA GCCAACCACA CCCTGAAGTA CGGCACCCAG
GCCAGGAAGT TTGATGTGAA CCAGTTGCAG AACACCACTA TCAAGCGGAT CATAAAGAAG
GTTCAGGACC TAGAACGGGC AGCACTGCCT GCCCAGGAGC TGGAGGAGTA CAACAAGATC
CTGTTGGATA TGGAAACCAC CTACAGCGTG GCCACTGTGT GCCACCCGAA TGGCAGCTGC
CTGCAGCTCG AGCCAGATCT GACGAATGTG ATGGCCACGT CCCGGAAATA TGAAGACCTG
TTATGGGCAT GGGAGGGCTG GCGAGACAAG GCGGGGAGAG CCATCCTCCA GTTTTACCCG
AAATACGTGG AACTCATCAA CCAGGCTGCC CGGCTCAATG GCTATGTAGA TGCAGGGGAC
TCGTGGAGGT CTATGTACGA GACACCATCC CTGGAGCAAG ACCTGGAGCG GCTCTTCCAG
GAGCTGCAGC CACTCTACCT CAACCTGCAT GCCTACGTGC GCCGGGCCCT GCACCGTCAC
TACGGGGCCC AGCACATCAA CCTGGAGGGG CCCATTCCTG CTCACCTGCT GGGGAACATG
TGGGCGCAGA CCTGGTCCAA CATCTATGAC TTGGTGGTGC CCTTCCCTTC AGCCCCCTCG
ATGGACACCA CAGAGGCTAT GCTAAAGCAG GGCTGGACGC CCAGGAGGAT GTTTAAGGAG
GCTGATGATT TCTTCACCTC CCTGGGGCTG CTGCCCGTGC CTCCTGAGTT CTGGAACAAG
TCGATGCTGG AGAAGCCAAC CGACGGGCGG GAGGTGGTCT GCCACGCCTC GGCCTGGGAC
TTCTACAACG GCAAGGACTT CCGGATCAAG CAGTGCACCA CCGTGAACTT GGAGGACCTG
GTGGTGGCCC ACCACGAAAT GGGCCACATC CAGTATTTCA TGCAGTACAA AGACTTACCT
GTGGCCTTGA GGGAGGGTGC CAACCCCGGC TTCCATGAGG CCATTGGGGA CGTGCTAGCC
CTCTCAGTGT CTACGCCCAA GCACCTGCAC AGTCTCAACC TGCTGAGCAG TGAGGGTGGC
AGCGACGAGC ATGACATCAA CTTTCTGATG AAGATGGCCC TTGACAAGAT CGCCTTTATC
CCCTTCAGCT ACCTCGTCGA TCAGTGGCGC TGGAGGGTAT TTGATGGAAG CATCACCAAG
GAGAACTATA ACCAGGAGTG GTGGAGCCTC AGGCTGAAGT ACCAGGGCCT CTGCCCCCCA
GTGCCCAGGA CTCAAGGTGA CTTTGACCCA GGGGCCAAGT TCCACATTCC TTCTAGCGTG
CCTTACATCA GGTACTTTGT CAGCTTCATC ATCCAGTTCC AGTTCCACGA GGCACTGTGC
CAGGCAGCTG GCCACACGGG CCCCCTGCAC AAGTGTGACA TCTACCAGTC CAAGGAGGCC
GGGCAGCGCC TGGCGACCGC CATGAAGCTG GGCTTCAGTA GGCCGTGGCC GGAAGCCATG
CAGCTGATCA CGGGCCAGCC CAACATGAGC GCCTCGGCCA TGTTGAGCTA CTTCAAGCCG
CTGCTGGACT GGCTCCGCAC GGAGAACGAG CTGCATGGGG AGAAGCTGGG CTGGCCGCAG
TACAACTGGA CGCCGAACTC CGCTCGCTCA GAAGGGCCCC TCCCAGACAG CGGCCGCGTC
AGCTTCCTGG GCCTGGACCT GGATGCGCAG CAGGCCCGCG TGGGCCAGTG GCTGCTGCTC
TTCCTGGGCA TCGCCCTGCT GGTAGCCACC CTGGGCCTCA GCCAGCGGCT CTTCAGCATC
CGCCACCGCA GCCTCCACCG GCACTCCCAC GGGCCCCAGT TCGGCTCCGA GGTGGAGCTG
AGACACTCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57207
Clone ID Related Accession (Same CDS sequence) XM_006721737
Accession Version XM_006721737.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2259bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product angiotensin-converting enzyme isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578830519. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGTCTTTCC CCCAGCATCC TAGAGAGGGT GTGCTCAGAC CTGAGGGCCC CTCCCCTTCC 
AGAGGAAGCC AGACACAAGG CTCTGTGAGG TCACACTGCG GGCTCCGCTC TTATTGGCCA
GGGGACGGTA GCTGCAGGAC TCTGCTCTCC TGCGGCCATG GGCCAGGGTT GGGCTACTGC
AGGACTTCCC AGCCTCCTCT TCCTGCTGCT CTGCTACGGG CACCCTCTGC TGGTCCCCAG
CCAGGAGGCA TCCCAACAGA CCTGGTGACT GATGAGGCTG AGGCCAGCAA GTTTGTGGAG
GAATATGACC GGACATCCCA GGTGGTGTGG AACGAGTATG CCGAGGCCAA CTGGAACTAC
AACACCAACA TCACCACAGA GACCAGCAAG ATTCTGCTGC AGAAGAACAT GCAAATAGCC
AACCACACCC TGAAGTACGG CACCCAGGCC AGGAAGTTTG ATGTGAACCA GTTGCAGAAC
ACCACTATCA AGCGGATCAT AAAGAAGGTT CAGGACCTAG AACGGGCAGC ACTGCCTGCC
CAGGAGCTGG AGGAGTACAA CAAGATCCTG TTGGATATGG AAACCACCTA CAGCGTGGCC
ACTGTGTGCC ACCCGAATGG CAGCTGCCTG CAGCTCGAGC CAGATCTGAC GAATGTGATG
GCCACGTCCC GGAAATATGA AGACCTGTTA TGGGCATGGG AGGGCTGGCG AGACAAGGCG
GGGAGAGCCA TCCTCCAGTT TTACCCGAAA TACGTGGAAC TCATCAACCA GGCTGCCCGG
CTCAATGGCT ATGTAGATGC AGGGGACTCG TGGAGGTCTA TGTACGAGAC ACCATCCCTG
GAGCAAGACC TGGAGCGGCT CTTCCAGGAG CTGCAGCCAC TCTACCTCAA CCTGCATGCC
TACGTGCGCC GGGCCCTGCA CCGTCACTAC GGGGCCCAGC ACATCAACCT GGAGGGGCCC
ATTCCTGCTC ACCTGCTGGG GAACATGTGG GCGCAGACCT GGTCCAACAT CTATGACTTG
GTGGTGCCCT TCCCTTCAGC CCCCTCGATG GACACCACAG AGGCTATGCT AAAGCAGGGC
TGGACGCCCA GGAGGATGTT TAAGGAGGCT GATGATTTCT TCACCTCCCT GGGGCTGCTG
CCCGTGCCTC CTGAGTTCTG GAACAAGTCG ATGCTGGAGA AGCCAACCGA CGGGCGGGAG
GTGGTCTGCC ACGCCTCGGC CTGGGACTTC TACAACGGCA AGGACTTCCG GATCAAGCAG
TGCACCACCG TGAACTTGGA GGACCTGGTG GTGGCCCACC ACGAAATGGG CCACATCCAG
TATTTCATGC AGTACAAAGA CTTACCTGTG GCCTTGAGGG AGGGTGCCAA CCCCGGCTTC
CATGAGGCCA TTGGGGACGT GCTAGCCCTC TCAGTGTCTA CGCCCAAGCA CCTGCACAGT
CTCAACCTGC TGAGCAGTGA GGGTGGCAGC GACGAGCATG ACATCAACTT TCTGATGAAG
ATGGCCCTTG ACAAGATCGC CTTTATCCCC TTCAGCTACC TCGTCGATCA GTGGCGCTGG
AGGGTATTTG ATGGAAGCAT CACCAAGGAG AACTATAACC AGGAGTGGTG GAGCCTCAGG
CTGAAGTACC AGGGCCTCTG CCCCCCAGTG CCCAGGACTC AAGGTGACTT TGACCCAGGG
GCCAAGTTCC ACATTCCTTC TAGCGTGCCT TACATCAGGT ACTTTGTCAG CTTCATCATC
CAGTTCCAGT TCCACGAGGC ACTGTGCCAG GCAGCTGGCC ACACGGGCCC CCTGCACAAG
TGTGACATCT ACCAGTCCAA GGAGGCCGGG CAGCGCCTGG CGACCGCCAT GAAGCTGGGC
TTCAGTAGGC CGTGGCCGGA AGCCATGCAG CTGATCACGG GCCAGCCCAA CATGAGCGCC
TCGGCCATGT TGAGCTACTT CAAGCCGCTG CTGGACTGGC TCCGCACGGA GAACGAGCTG
CATGGGGAGA AGCTGGGCTG GCCGCAGTAC AACTGGACGC CGAACTCCGC TCGCTCAGAA
GGGCCCCTCC CAGACAGCGG CCGCGTCAGC TTCCTGGGCC TGGACCTGGA TGCGCAGCAG
GCCCGCGTGG GCCAGTGGCT GCTGCTCTTC CTGGGCATCG CCCTGCTGGT AGCCACCCTG
GGCCTCAGCC AGCGGCTCTT CAGCATCCGC CACCGCAGCC TCCACCGGCA CTCCCACGGG
CCCCAGTTCG GCTCCGAGGT GGAGCTGAGA CACTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006721800.2
CDS94..2352
Misc Feature(1)385..2067(+)
Misc Feature(2)1303..1815(+)
Misc Feature(3)1393..1479(+)
Translation

Target ORF information:

RefSeq Version XM_006721737
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens angiotensin I converting enzyme (ACE), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006721737

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGTCTTTCC CCCAGCATCC TAGAGAGGGT GTGCTCAGAC CTGAGGGCCC CTCCCCTTCC 
AGAGGAAGCC AGACACAAGG CTCTGTGAGG TCACACTGCG GGCTCCGCTC TTATTGGCCA
GGGGACGGTA GCTGCAGGAC TCTGCTCTCC TGCGGCCATG GGCCAGGGTT GGGCTACTGC
AGGACTTCCC AGCCTCCTCT TCCTGCTGCT CTGCTACGGG CACCCTCTGC TGGTCCCCAG
CCAGGAGGCA TCCCAACAGA CCTGGTGACT GATGAGGCTG AGGCCAGCAA GTTTGTGGAG
GAATATGACC GGACATCCCA GGTGGTGTGG AACGAGTATG CCGAGGCCAA CTGGAACTAC
AACACCAACA TCACCACAGA GACCAGCAAG ATTCTGCTGC AGAAGAACAT GCAAATAGCC
AACCACACCC TGAAGTACGG CACCCAGGCC AGGAAGTTTG ATGTGAACCA GTTGCAGAAC
ACCACTATCA AGCGGATCAT AAAGAAGGTT CAGGACCTAG AACGGGCAGC ACTGCCTGCC
CAGGAGCTGG AGGAGTACAA CAAGATCCTG TTGGATATGG AAACCACCTA CAGCGTGGCC
ACTGTGTGCC ACCCGAATGG CAGCTGCCTG CAGCTCGAGC CAGATCTGAC GAATGTGATG
GCCACGTCCC GGAAATATGA AGACCTGTTA TGGGCATGGG AGGGCTGGCG AGACAAGGCG
GGGAGAGCCA TCCTCCAGTT TTACCCGAAA TACGTGGAAC TCATCAACCA GGCTGCCCGG
CTCAATGGCT ATGTAGATGC AGGGGACTCG TGGAGGTCTA TGTACGAGAC ACCATCCCTG
GAGCAAGACC TGGAGCGGCT CTTCCAGGAG CTGCAGCCAC TCTACCTCAA CCTGCATGCC
TACGTGCGCC GGGCCCTGCA CCGTCACTAC GGGGCCCAGC ACATCAACCT GGAGGGGCCC
ATTCCTGCTC ACCTGCTGGG GAACATGTGG GCGCAGACCT GGTCCAACAT CTATGACTTG
GTGGTGCCCT TCCCTTCAGC CCCCTCGATG GACACCACAG AGGCTATGCT AAAGCAGGGC
TGGACGCCCA GGAGGATGTT TAAGGAGGCT GATGATTTCT TCACCTCCCT GGGGCTGCTG
CCCGTGCCTC CTGAGTTCTG GAACAAGTCG ATGCTGGAGA AGCCAACCGA CGGGCGGGAG
GTGGTCTGCC ACGCCTCGGC CTGGGACTTC TACAACGGCA AGGACTTCCG GATCAAGCAG
TGCACCACCG TGAACTTGGA GGACCTGGTG GTGGCCCACC ACGAAATGGG CCACATCCAG
TATTTCATGC AGTACAAAGA CTTACCTGTG GCCTTGAGGG AGGGTGCCAA CCCCGGCTTC
CATGAGGCCA TTGGGGACGT GCTAGCCCTC TCAGTGTCTA CGCCCAAGCA CCTGCACAGT
CTCAACCTGC TGAGCAGTGA GGGTGGCAGC GACGAGCATG ACATCAACTT TCTGATGAAG
ATGGCCCTTG ACAAGATCGC CTTTATCCCC TTCAGCTACC TCGTCGATCA GTGGCGCTGG
AGGGTATTTG ATGGAAGCAT CACCAAGGAG AACTATAACC AGGAGTGGTG GAGCCTCAGG
CTGAAGTACC AGGGCCTCTG CCCCCCAGTG CCCAGGACTC AAGGTGACTT TGACCCAGGG
GCCAAGTTCC ACATTCCTTC TAGCGTGCCT TACATCAGGT ACTTTGTCAG CTTCATCATC
CAGTTCCAGT TCCACGAGGC ACTGTGCCAG GCAGCTGGCC ACACGGGCCC CCTGCACAAG
TGTGACATCT ACCAGTCCAA GGAGGCCGGG CAGCGCCTGG CGACCGCCAT GAAGCTGGGC
TTCAGTAGGC CGTGGCCGGA AGCCATGCAG CTGATCACGG GCCAGCCCAA CATGAGCGCC
TCGGCCATGT TGAGCTACTT CAAGCCGCTG CTGGACTGGC TCCGCACGGA GAACGAGCTG
CATGGGGAGA AGCTGGGCTG GCCGCAGTAC AACTGGACGC CGAACTCCGC TCGCTCAGAA
GGGCCCCTCC CAGACAGCGG CCGCGTCAGC TTCCTGGGCC TGGACCTGGA TGCGCAGCAG
GCCCGCGTGG GCCAGTGGCT GCTGCTCTTC CTGGGCATCG CCCTGCTGGT AGCCACCCTG
GGCCTCAGCC AGCGGCTCTT CAGCATCCGC CACCGCAGCC TCCACCGGCA CTCCCACGGG
CCCCAGTTCG GCTCCGAGGT GGAGCTGAGA CACTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20567
Clone ID Related Accession (Same CDS sequence) NM_000789
Accession Version NM_000789.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3921bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product angiotensin-converting enzyme isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK296566.1, AB208971.1 and AC113554.9. This sequence is a reference standard in the RefSeqGene project. On Sep 25, 2010 this sequence version replaced gi:23238217. Transcript Variant: This variant (1), also known as the endothelial or somatic form, represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J04144.1, AB208971.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 5' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
ATGGGGGCCG CCTCGGGCCG CCGGGGGCCG GGGCTGCTGC TGCCGCTGCC GCTGCTGTTG 
CTGCTGCCGC CGCAGCCCGC CCTGGCGTTG GACCCCGGGC TGCAGCCCGG CAACTTTTCT
GCTGACGAGG CCGGGGCGCA GCTCTTCGCG CAGAGCTACA ACTCCAGCGC CGAACAGGTG
CTGTTCCAGA GCGTGGCCGC CAGCTGGGCG CACGACACCA ACATCACCGC GGAGAATGCA
AGGCGCCAGG AGGAAGCAGC CCTGCTCAGC CAGGAGTTTG CGGAGGCCTG GGGCCAGAAG
GCCAAGGAGC TGTATGAACC GATCTGGCAG AACTTCACGG ACCCGCAGCT GCGCAGGATC
ATCGGAGCTG TGCGCACCCT GGGCTCTGCC AACCTGCCCC TGGCTAAGCG GCAGCAGTAC
AACGCCCTGC TAAGCAACAT GAGCAGGATC TACTCCACCG CCAAGGTCTG CCTCCCCAAC
AAGACTGCCA CCTGCTGGTC CCTGGACCCA GATCTCACCA ACATCCTGGC TTCCTCGCGA
AGCTACGCCA TGCTCCTGTT TGCCTGGGAG GGCTGGCACA ACGCTGCGGG CATCCCGCTG
AAACCGCTGT ACGAGGATTT CACTGCCCTC AGCAATGAAG CCTACAAGCA GGACGGCTTC
ACAGACACGG GGGCCTACTG GCGCTCCTGG TACAACTCCC CCACCTTCGA GGACGATCTG
GAACACCTCT ACCAACAGCT AGAGCCCCTC TACCTGAACC TCCATGCCTT CGTCCGCCGC
GCACTGCATC GCCGATACGG AGACAGATAC ATCAACCTCA GGGGACCCAT CCCTGCTCAT
CTGCTGGGAG ACATGTGGGC CCAGAGCTGG GAAAACATCT ACGACATGGT GGTGCCTTTC
CCAGACAAGC CCAACCTCGA TGTCACCAGT ACTATGCTGC AGCAGGGCTG GAACGCCACG
CACATGTTCC GGGTGGCAGA GGAGTTCTTC ACCTCCCTGG AGCTCTCCCC CATGCCTCCC
GAGTTCTGGG AAGGGTCGAT GCTGGAGAAG CCGGCCGACG GGCGGGAAGT GGTGTGCCAC
GCCTCGGCTT GGGACTTCTA CAACAGGAAA GACTTCAGGA TCAAGCAGTG CACACGGGTC
ACGATGGACC AGCTCTCCAC AGTGCACCAT GAGATGGGCC ATATACAGTA CTACCTGCAG
TACAAGGATC TGCCCGTCTC CCTGCGTCGG GGGGCCAACC CCGGCTTCCA TGAGGCCATT
GGGGACGTGC TGGCGCTCTC GGTCTCCACT CCTGAACATC TGCACAAAAT CGGCCTGCTG
GACCGTGTCA CCAATGACAC GGAAAGTGAC ATCAATTACT TGCTAAAAAT GGCACTGGAA
AAAATTGCCT TCCTGCCCTT TGGCTACTTG GTGGACCAGT GGCGCTGGGG GGTCTTTAGT
GGGCGTACCC CCCCTTCCCG CTACAACTTC GACTGGTGGT ATCTTCGAAC CAAGTATCAG
GGGATCTGTC CTCCTGTTAC CCGAAACGAA ACCCACTTTG ATGCTGGAGC TAAGTTTCAT
GTTCCAAATG TGACACCATA CATCAGGTAC TTTGTGAGTT TTGTCCTGCA GTTCCAGTTC
CATGAAGCCC TGTGCAAGGA GGCAGGCTAT GAGGGCCCAC TGCACCAGTG TGACATCTAC
CGGTCCACCA AGGCAGGGGC CAAGCTCCGG AAGGTGCTGC AGGCTGGCTC CTCCAGGCCC
TGGCAGGAGG TGCTGAAGGA CATGGTCGGC TTAGATGCCC TGGATGCCCA GCCGCTGCTC
AAGTACTTCC AGCCAGTCAC CCAGTGGCTG CAGGAGCAGA ACCAGCAGAA CGGCGAGGTC
CTGGGCTGGC CCGAGTACCA GTGGCACCCG CCGTTGCCTG ACAACTACCC GGAGGGCATA
GACCTGGTGA CTGATGAGGC TGAGGCCAGC AAGTTTGTGG AGGAATATGA CCGGACATCC
CAGGTGGTGT GGAACGAGTA TGCCGAGGCC AACTGGAACT ACAACACCAA CATCACCACA
GAGACCAGCA AGATTCTGCT GCAGAAGAAC ATGCAAATAG CCAACCACAC CCTGAAGTAC
GGCACCCAGG CCAGGAAGTT TGATGTGAAC CAGTTGCAGA ACACCACTAT CAAGCGGATC
ATAAAGAAGG TTCAGGACCT AGAACGGGCA GCACTGCCTG CCCAGGAGCT GGAGGAGTAC
AACAAGATCC TGTTGGATAT GGAAACCACC TACAGCGTGG CCACTGTGTG CCACCCGAAT
GGCAGCTGCC TGCAGCTCGA GCCAGATCTG ACGAATGTGA TGGCCACGTC CCGGAAATAT
GAAGACCTGT TATGGGCATG GGAGGGCTGG CGAGACAAGG CGGGGAGAGC CATCCTCCAG
TTTTACCCGA AATACGTGGA ACTCATCAAC CAGGCTGCCC GGCTCAATGG CTATGTAGAT
GCAGGGGACT CGTGGAGGTC TATGTACGAG ACACCATCCC TGGAGCAAGA CCTGGAGCGG
CTCTTCCAGG AGCTGCAGCC ACTCTACCTC AACCTGCATG CCTACGTGCG CCGGGCCCTG
CACCGTCACT ACGGGGCCCA GCACATCAAC CTGGAGGGGC CCATTCCTGC TCACCTGCTG
GGGAACATGT GGGCGCAGAC CTGGTCCAAC ATCTATGACT TGGTGGTGCC CTTCCCTTCA
GCCCCCTCGA TGGACACCAC AGAGGCTATG CTAAAGCAGG GCTGGACGCC CAGGAGGATG
TTTAAGGAGG CTGATGATTT CTTCACCTCC CTGGGGCTGC TGCCCGTGCC TCCTGAGTTC
TGGAACAAGT CGATGCTGGA GAAGCCAACC GACGGGCGGG AGGTGGTCTG CCACGCCTCG
GCCTGGGACT TCTACAACGG CAAGGACTTC CGGATCAAGC AGTGCACCAC CGTGAACTTG
GAGGACCTGG TGGTGGCCCA CCACGAAATG GGCCACATCC AGTATTTCAT GCAGTACAAA
GACTTACCTG TGGCCTTGAG GGAGGGTGCC AACCCCGGCT TCCATGAGGC CATTGGGGAC
GTGCTAGCCC TCTCAGTGTC TACGCCCAAG CACCTGCACA GTCTCAACCT GCTGAGCAGT
GAGGGTGGCA GCGACGAGCA TGACATCAAC TTTCTGATGA AGATGGCCCT TGACAAGATC
GCCTTTATCC CCTTCAGCTA CCTCGTCGAT CAGTGGCGCT GGAGGGTATT TGATGGAAGC
ATCACCAAGG AGAACTATAA CCAGGAGTGG TGGAGCCTCA GGCTGAAGTA CCAGGGCCTC
TGCCCCCCAG TGCCCAGGAC TCAAGGTGAC TTTGACCCAG GGGCCAAGTT CCACATTCCT
TCTAGCGTGC CTTACATCAG GTACTTTGTC AGCTTCATCA TCCAGTTCCA GTTCCACGAG
GCACTGTGCC AGGCAGCTGG CCACACGGGC CCCCTGCACA AGTGTGACAT CTACCAGTCC
AAGGAGGCCG GGCAGCGCCT GGCGACCGCC ATGAAGCTGG GCTTCAGTAG GCCGTGGCCG
GAAGCCATGC AGCTGATCAC GGGCCAGCCC AACATGAGCG CCTCGGCCAT GTTGAGCTAC
TTCAAGCCGC TGCTGGACTG GCTCCGCACG GAGAACGAGC TGCATGGGGA GAAGCTGGGC
TGGCCGCAGT ACAACTGGAC GCCGAACTCC GCTCGCTCAG AAGGGCCCCT CCCAGACAGC
GGCCGCGTCA GCTTCCTGGG CCTGGACCTG GATGCGCAGC AGGCCCGCGT GGGCCAGTGG
CTGCTGCTCT TCCTGGGCAT CGCCCTGCTG GTAGCCACCC TGGGCCTCAG CCAGCGGCTC
TTCAGCATCC GCCACCGCAG CCTCCACCGG CACTCCCACG GGCCCCAGTT CGGCTCCGAG
GTGGAGCTGA GACACTCCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000780.1
CDS35..3955
Misc Feature(1)122..1924(+)
Misc Feature(2)179..1876(+)
Misc Feature(3)1112..1624(+)
Misc Feature(4)1202..1288(+)
Misc Feature(5)1925..3730(+)
Misc Feature(6)1988..3670(+)
Misc Feature(7)2906..3418(+)
Misc Feature(8)2996..3082(+)
Misc Feature(9)3530..3532(+)
Misc Feature(10)3707..3709(+)
Misc Feature(11)3728..3730(+)
Misc Feature(12)3803..3865(+)
Misc Feature(13)3929..3931(+)
Exon (1)1..283
Gene:ACE
Gene Synonym:
Exon (2)284..451
Gene:ACE
Gene Synonym:
Exon (3)452..545
Gene:ACE
Gene Synonym:
Exon (4)546..689
Gene:ACE
Gene Synonym:
Exon (5)690..881
Gene:ACE
Gene Synonym:
Exon (6)882..979
Gene:ACE
Gene Synonym:
Exon (7)980..1152
Gene:ACE
Gene Synonym:
Exon (8)1153..1376
Gene:ACE
Gene Synonym:
Exon (9)1377..1521
Gene:ACE
Gene Synonym:
Exon (10)1522..1620
Gene:ACE
Gene Synonym:
Exon (11)1621..1743
Gene:ACE
Gene Synonym:
Exon (12)1744..1955
Gene:ACE
Gene Synonym:
Exon (13)1956..2092
Gene:ACE
Gene Synonym:
Exon (14)2093..2251
Gene:ACE
Gene Synonym:
Exon (15)2252..2339
Gene:ACE
Gene Synonym:
Exon (16)2340..2483
Gene:ACE
Gene Synonym:
Exon (17)2484..2675
Gene:ACE
Gene Synonym:
Exon (18)2676..2773
Gene:ACE
Gene Synonym:
Exon (19)2774..2946
Gene:ACE
Gene Synonym:
Exon (20)2947..3170
Gene:ACE
Gene Synonym:
Exon (21)3171..3315
Gene:ACE
Gene Synonym:
Exon (22)3316..3414
Gene:ACE
Gene Synonym:
Exon (23)3415..3537
Gene:ACE
Gene Synonym:
Exon (24)3538..3725
Gene:ACE
Gene Synonym:
Exon (25)3726..4969
Gene:ACE
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000789
Organism Homo sapiens (human)
Definition Homo sapiens angiotensin I converting enzyme (ACE), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000789

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
ATGGGGGCCG CCTCGGGCCG CCGGGGGCCG GGGCTGCTGC TGCCGCTGCC GCTGCTGTTG 
CTGCTGCCGC CGCAGCCCGC CCTGGCGTTG GACCCCGGGC TGCAGCCCGG CAACTTTTCT
GCTGACGAGG CCGGGGCGCA GCTCTTCGCG CAGAGCTACA ACTCCAGCGC CGAACAGGTG
CTGTTCCAGA GCGTGGCCGC CAGCTGGGCG CACGACACCA ACATCACCGC GGAGAATGCA
AGGCGCCAGG AGGAAGCAGC CCTGCTCAGC CAGGAGTTTG CGGAGGCCTG GGGCCAGAAG
GCCAAGGAGC TGTATGAACC GATCTGGCAG AACTTCACGG ACCCGCAGCT GCGCAGGATC
ATCGGAGCTG TGCGCACCCT GGGCTCTGCC AACCTGCCCC TGGCTAAGCG GCAGCAGTAC
AACGCCCTGC TAAGCAACAT GAGCAGGATC TACTCCACCG CCAAGGTCTG CCTCCCCAAC
AAGACTGCCA CCTGCTGGTC CCTGGACCCA GATCTCACCA ACATCCTGGC TTCCTCGCGA
AGCTACGCCA TGCTCCTGTT TGCCTGGGAG GGCTGGCACA ACGCTGCGGG CATCCCGCTG
AAACCGCTGT ACGAGGATTT CACTGCCCTC AGCAATGAAG CCTACAAGCA GGACGGCTTC
ACAGACACGG GGGCCTACTG GCGCTCCTGG TACAACTCCC CCACCTTCGA GGACGATCTG
GAACACCTCT ACCAACAGCT AGAGCCCCTC TACCTGAACC TCCATGCCTT CGTCCGCCGC
GCACTGCATC GCCGATACGG AGACAGATAC ATCAACCTCA GGGGACCCAT CCCTGCTCAT
CTGCTGGGAG ACATGTGGGC CCAGAGCTGG GAAAACATCT ACGACATGGT GGTGCCTTTC
CCAGACAAGC CCAACCTCGA TGTCACCAGT ACTATGCTGC AGCAGGGCTG GAACGCCACG
CACATGTTCC GGGTGGCAGA GGAGTTCTTC ACCTCCCTGG AGCTCTCCCC CATGCCTCCC
GAGTTCTGGG AAGGGTCGAT GCTGGAGAAG CCGGCCGACG GGCGGGAAGT GGTGTGCCAC
GCCTCGGCTT GGGACTTCTA CAACAGGAAA GACTTCAGGA TCAAGCAGTG CACACGGGTC
ACGATGGACC AGCTCTCCAC AGTGCACCAT GAGATGGGCC ATATACAGTA CTACCTGCAG
TACAAGGATC TGCCCGTCTC CCTGCGTCGG GGGGCCAACC CCGGCTTCCA TGAGGCCATT
GGGGACGTGC TGGCGCTCTC GGTCTCCACT CCTGAACATC TGCACAAAAT CGGCCTGCTG
GACCGTGTCA CCAATGACAC GGAAAGTGAC ATCAATTACT TGCTAAAAAT GGCACTGGAA
AAAATTGCCT TCCTGCCCTT TGGCTACTTG GTGGACCAGT GGCGCTGGGG GGTCTTTAGT
GGGCGTACCC CCCCTTCCCG CTACAACTTC GACTGGTGGT ATCTTCGAAC CAAGTATCAG
GGGATCTGTC CTCCTGTTAC CCGAAACGAA ACCCACTTTG ATGCTGGAGC TAAGTTTCAT
GTTCCAAATG TGACACCATA CATCAGGTAC TTTGTGAGTT TTGTCCTGCA GTTCCAGTTC
CATGAAGCCC TGTGCAAGGA GGCAGGCTAT GAGGGCCCAC TGCACCAGTG TGACATCTAC
CGGTCCACCA AGGCAGGGGC CAAGCTCCGG AAGGTGCTGC AGGCTGGCTC CTCCAGGCCC
TGGCAGGAGG TGCTGAAGGA CATGGTCGGC TTAGATGCCC TGGATGCCCA GCCGCTGCTC
AAGTACTTCC AGCCAGTCAC CCAGTGGCTG CAGGAGCAGA ACCAGCAGAA CGGCGAGGTC
CTGGGCTGGC CCGAGTACCA GTGGCACCCG CCGTTGCCTG ACAACTACCC GGAGGGCATA
GACCTGGTGA CTGATGAGGC TGAGGCCAGC AAGTTTGTGG AGGAATATGA CCGGACATCC
CAGGTGGTGT GGAACGAGTA TGCCGAGGCC AACTGGAACT ACAACACCAA CATCACCACA
GAGACCAGCA AGATTCTGCT GCAGAAGAAC ATGCAAATAG CCAACCACAC CCTGAAGTAC
GGCACCCAGG CCAGGAAGTT TGATGTGAAC CAGTTGCAGA ACACCACTAT CAAGCGGATC
ATAAAGAAGG TTCAGGACCT AGAACGGGCA GCACTGCCTG CCCAGGAGCT GGAGGAGTAC
AACAAGATCC TGTTGGATAT GGAAACCACC TACAGCGTGG CCACTGTGTG CCACCCGAAT
GGCAGCTGCC TGCAGCTCGA GCCAGATCTG ACGAATGTGA TGGCCACGTC CCGGAAATAT
GAAGACCTGT TATGGGCATG GGAGGGCTGG CGAGACAAGG CGGGGAGAGC CATCCTCCAG
TTTTACCCGA AATACGTGGA ACTCATCAAC CAGGCTGCCC GGCTCAATGG CTATGTAGAT
GCAGGGGACT CGTGGAGGTC TATGTACGAG ACACCATCCC TGGAGCAAGA CCTGGAGCGG
CTCTTCCAGG AGCTGCAGCC ACTCTACCTC AACCTGCATG CCTACGTGCG CCGGGCCCTG
CACCGTCACT ACGGGGCCCA GCACATCAAC CTGGAGGGGC CCATTCCTGC TCACCTGCTG
GGGAACATGT GGGCGCAGAC CTGGTCCAAC ATCTATGACT TGGTGGTGCC CTTCCCTTCA
GCCCCCTCGA TGGACACCAC AGAGGCTATG CTAAAGCAGG GCTGGACGCC CAGGAGGATG
TTTAAGGAGG CTGATGATTT CTTCACCTCC CTGGGGCTGC TGCCCGTGCC TCCTGAGTTC
TGGAACAAGT CGATGCTGGA GAAGCCAACC GACGGGCGGG AGGTGGTCTG CCACGCCTCG
GCCTGGGACT TCTACAACGG CAAGGACTTC CGGATCAAGC AGTGCACCAC CGTGAACTTG
GAGGACCTGG TGGTGGCCCA CCACGAAATG GGCCACATCC AGTATTTCAT GCAGTACAAA
GACTTACCTG TGGCCTTGAG GGAGGGTGCC AACCCCGGCT TCCATGAGGC CATTGGGGAC
GTGCTAGCCC TCTCAGTGTC TACGCCCAAG CACCTGCACA GTCTCAACCT GCTGAGCAGT
GAGGGTGGCA GCGACGAGCA TGACATCAAC TTTCTGATGA AGATGGCCCT TGACAAGATC
GCCTTTATCC CCTTCAGCTA CCTCGTCGAT CAGTGGCGCT GGAGGGTATT TGATGGAAGC
ATCACCAAGG AGAACTATAA CCAGGAGTGG TGGAGCCTCA GGCTGAAGTA CCAGGGCCTC
TGCCCCCCAG TGCCCAGGAC TCAAGGTGAC TTTGACCCAG GGGCCAAGTT CCACATTCCT
TCTAGCGTGC CTTACATCAG GTACTTTGTC AGCTTCATCA TCCAGTTCCA GTTCCACGAG
GCACTGTGCC AGGCAGCTGG CCACACGGGC CCCCTGCACA AGTGTGACAT CTACCAGTCC
AAGGAGGCCG GGCAGCGCCT GGCGACCGCC ATGAAGCTGG GCTTCAGTAG GCCGTGGCCG
GAAGCCATGC AGCTGATCAC GGGCCAGCCC AACATGAGCG CCTCGGCCAT GTTGAGCTAC
TTCAAGCCGC TGCTGGACTG GCTCCGCACG GAGAACGAGC TGCATGGGGA GAAGCTGGGC
TGGCCGCAGT ACAACTGGAC GCCGAACTCC GCTCGCTCAG AAGGGCCCCT CCCAGACAGC
GGCCGCGTCA GCTTCCTGGG CCTGGACCTG GATGCGCAGC AGGCCCGCGT GGGCCAGTGG
CTGCTGCTCT TCCTGGGCAT CGCCCTGCTG GTAGCCACCC TGGGCCTCAG CCAGCGGCTC
TTCAGCATCC GCCACCGCAG CCTCCACCGG CACTCCCACG GGCCCCAGTT CGGCTCCGAG
GTGGAGCTGA GACACTCCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14691
Clone ID Related Accession (Same CDS sequence) NM_152830
Accession Version NM_152830.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2199bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product angiotensin-converting enzyme isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM908222.1, M26657.1, AB208971.1 and AC113554.9. On Sep 25, 2010 this sequence version replaced gi:23238213. Transcript Variant: This variant (2), also known as the testicular form, lacks multiple 5' exons, but has an alternate 5' exon, compared to variant 1. The resulting isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X16295.1, M26657.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGGCCAGG GTTGGGCTAC TGCAGGACTT CCCAGCCTCC TCTTCCTGCT GCTCTGCTAC 
GGGCACCCTC TGCTGGTCCC CAGCCAGGAG GCATCCCAAC AGGTGACAGT CACCCATGGG
ACAAGCAGCC AGGCAACAAC CAGCAGCCAG ACAACCACCC ACCAGGCGAC GGCCCACCAG
ACATCAGCCC AGAGCCCAAA CCTGGTGACT GATGAGGCTG AGGCCAGCAA GTTTGTGGAG
GAATATGACC GGACATCCCA GGTGGTGTGG AACGAGTATG CCGAGGCCAA CTGGAACTAC
AACACCAACA TCACCACAGA GACCAGCAAG ATTCTGCTGC AGAAGAACAT GCAAATAGCC
AACCACACCC TGAAGTACGG CACCCAGGCC AGGAAGTTTG ATGTGAACCA GTTGCAGAAC
ACCACTATCA AGCGGATCAT AAAGAAGGTT CAGGACCTAG AACGGGCAGC ACTGCCTGCC
CAGGAGCTGG AGGAGTACAA CAAGATCCTG TTGGATATGG AAACCACCTA CAGCGTGGCC
ACTGTGTGCC ACCCGAATGG CAGCTGCCTG CAGCTCGAGC CAGATCTGAC GAATGTGATG
GCCACGTCCC GGAAATATGA AGACCTGTTA TGGGCATGGG AGGGCTGGCG AGACAAGGCG
GGGAGAGCCA TCCTCCAGTT TTACCCGAAA TACGTGGAAC TCATCAACCA GGCTGCCCGG
CTCAATGGCT ATGTAGATGC AGGGGACTCG TGGAGGTCTA TGTACGAGAC ACCATCCCTG
GAGCAAGACC TGGAGCGGCT CTTCCAGGAG CTGCAGCCAC TCTACCTCAA CCTGCATGCC
TACGTGCGCC GGGCCCTGCA CCGTCACTAC GGGGCCCAGC ACATCAACCT GGAGGGGCCC
ATTCCTGCTC ACCTGCTGGG GAACATGTGG GCGCAGACCT GGTCCAACAT CTATGACTTG
GTGGTGCCCT TCCCTTCAGC CCCCTCGATG GACACCACAG AGGCTATGCT AAAGCAGGGC
TGGACGCCCA GGAGGATGTT TAAGGAGGCT GATGATTTCT TCACCTCCCT GGGGCTGCTG
CCCGTGCCTC CTGAGTTCTG GAACAAGTCG ATGCTGGAGA AGCCAACCGA CGGGCGGGAG
GTGGTCTGCC ACGCCTCGGC CTGGGACTTC TACAACGGCA AGGACTTCCG GATCAAGCAG
TGCACCACCG TGAACTTGGA GGACCTGGTG GTGGCCCACC ACGAAATGGG CCACATCCAG
TATTTCATGC AGTACAAAGA CTTACCTGTG GCCTTGAGGG AGGGTGCCAA CCCCGGCTTC
CATGAGGCCA TTGGGGACGT GCTAGCCCTC TCAGTGTCTA CGCCCAAGCA CCTGCACAGT
CTCAACCTGC TGAGCAGTGA GGGTGGCAGC GACGAGCATG ACATCAACTT TCTGATGAAG
ATGGCCCTTG ACAAGATCGC CTTTATCCCC TTCAGCTACC TCGTCGATCA GTGGCGCTGG
AGGGTATTTG ATGGAAGCAT CACCAAGGAG AACTATAACC AGGAGTGGTG GAGCCTCAGG
CTGAAGTACC AGGGCCTCTG CCCCCCAGTG CCCAGGACTC AAGGTGACTT TGACCCAGGG
GCCAAGTTCC ACATTCCTTC TAGCGTGCCT TACATCAGGT ACTTTGTCAG CTTCATCATC
CAGTTCCAGT TCCACGAGGC ACTGTGCCAG GCAGCTGGCC ACACGGGCCC CCTGCACAAG
TGTGACATCT ACCAGTCCAA GGAGGCCGGG CAGCGCCTGG CGACCGCCAT GAAGCTGGGC
TTCAGTAGGC CGTGGCCGGA AGCCATGCAG CTGATCACGG GCCAGCCCAA CATGAGCGCC
TCGGCCATGT TGAGCTACTT CAAGCCGCTG CTGGACTGGC TCCGCACGGA GAACGAGCTG
CATGGGGAGA AGCTGGGCTG GCCGCAGTAC AACTGGACGC CGAACTCCGC TCGCTCAGAA
GGGCCCCTCC CAGACAGCGG CCGCGTCAGC TTCCTGGGCC TGGACCTGGA TGCGCAGCAG
GCCCGCGTGG GCCAGTGGCT GCTGCTCTTC CTGGGCATCG CCCTGCTGGT AGCCACCCTG
GGCCTCAGCC AGCGGCTCTT CAGCATCCGC CACCGCAGCC TCCACCGGCA CTCCCACGGG
CCCCAGTTCG GCTCCGAGGT GGAGCTGAGA CACTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_690043.1
CDS52..2250
Misc Feature(1)283..1965(+)
Misc Feature(2)1201..1713(+)
Misc Feature(3)1291..1377(+)
Misc Feature(4)1825..1827(+)
Misc Feature(5)2023..2025(+)
Exon (1)1..250
Gene:ACE
Gene Synonym:
Exon (2)251..387
Gene:ACE
Gene Synonym:
Exon (3)388..546
Gene:ACE
Gene Synonym:
Exon (4)547..634
Gene:ACE
Gene Synonym:
Exon (5)635..778
Gene:ACE
Gene Synonym:
Exon (6)779..970
Gene:ACE
Gene Synonym:
Exon (7)971..1068
Gene:ACE
Gene Synonym:
Exon (8)1069..1241
Gene:ACE
Gene Synonym:
Exon (9)1242..1465
Gene:ACE
Gene Synonym:
Exon (10)1466..1610
Gene:ACE
Gene Synonym:
Exon (11)1611..1709
Gene:ACE
Gene Synonym:
Exon (12)1710..1832
Gene:ACE
Gene Synonym:
Exon (13)1833..2020
Gene:ACE
Gene Synonym:
Exon (14)2021..3264
Gene:ACE
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_152830
Organism Homo sapiens (human)
Definition Homo sapiens angiotensin I converting enzyme (ACE), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_152830

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGGCCAGG GTTGGGCTAC TGCAGGACTT CCCAGCCTCC TCTTCCTGCT GCTCTGCTAC 
GGGCACCCTC TGCTGGTCCC CAGCCAGGAG GCATCCCAAC AGGTGACAGT CACCCATGGG
ACAAGCAGCC AGGCAACAAC CAGCAGCCAG ACAACCACCC ACCAGGCGAC GGCCCACCAG
ACATCAGCCC AGAGCCCAAA CCTGGTGACT GATGAGGCTG AGGCCAGCAA GTTTGTGGAG
GAATATGACC GGACATCCCA GGTGGTGTGG AACGAGTATG CCGAGGCCAA CTGGAACTAC
AACACCAACA TCACCACAGA GACCAGCAAG ATTCTGCTGC AGAAGAACAT GCAAATAGCC
AACCACACCC TGAAGTACGG CACCCAGGCC AGGAAGTTTG ATGTGAACCA GTTGCAGAAC
ACCACTATCA AGCGGATCAT AAAGAAGGTT CAGGACCTAG AACGGGCAGC ACTGCCTGCC
CAGGAGCTGG AGGAGTACAA CAAGATCCTG TTGGATATGG AAACCACCTA CAGCGTGGCC
ACTGTGTGCC ACCCGAATGG CAGCTGCCTG CAGCTCGAGC CAGATCTGAC GAATGTGATG
GCCACGTCCC GGAAATATGA AGACCTGTTA TGGGCATGGG AGGGCTGGCG AGACAAGGCG
GGGAGAGCCA TCCTCCAGTT TTACCCGAAA TACGTGGAAC TCATCAACCA GGCTGCCCGG
CTCAATGGCT ATGTAGATGC AGGGGACTCG TGGAGGTCTA TGTACGAGAC ACCATCCCTG
GAGCAAGACC TGGAGCGGCT CTTCCAGGAG CTGCAGCCAC TCTACCTCAA CCTGCATGCC
TACGTGCGCC GGGCCCTGCA CCGTCACTAC GGGGCCCAGC ACATCAACCT GGAGGGGCCC
ATTCCTGCTC ACCTGCTGGG GAACATGTGG GCGCAGACCT GGTCCAACAT CTATGACTTG
GTGGTGCCCT TCCCTTCAGC CCCCTCGATG GACACCACAG AGGCTATGCT AAAGCAGGGC
TGGACGCCCA GGAGGATGTT TAAGGAGGCT GATGATTTCT TCACCTCCCT GGGGCTGCTG
CCCGTGCCTC CTGAGTTCTG GAACAAGTCG ATGCTGGAGA AGCCAACCGA CGGGCGGGAG
GTGGTCTGCC ACGCCTCGGC CTGGGACTTC TACAACGGCA AGGACTTCCG GATCAAGCAG
TGCACCACCG TGAACTTGGA GGACCTGGTG GTGGCCCACC ACGAAATGGG CCACATCCAG
TATTTCATGC AGTACAAAGA CTTACCTGTG GCCTTGAGGG AGGGTGCCAA CCCCGGCTTC
CATGAGGCCA TTGGGGACGT GCTAGCCCTC TCAGTGTCTA CGCCCAAGCA CCTGCACAGT
CTCAACCTGC TGAGCAGTGA GGGTGGCAGC GACGAGCATG ACATCAACTT TCTGATGAAG
ATGGCCCTTG ACAAGATCGC CTTTATCCCC TTCAGCTACC TCGTCGATCA GTGGCGCTGG
AGGGTATTTG ATGGAAGCAT CACCAAGGAG AACTATAACC AGGAGTGGTG GAGCCTCAGG
CTGAAGTACC AGGGCCTCTG CCCCCCAGTG CCCAGGACTC AAGGTGACTT TGACCCAGGG
GCCAAGTTCC ACATTCCTTC TAGCGTGCCT TACATCAGGT ACTTTGTCAG CTTCATCATC
CAGTTCCAGT TCCACGAGGC ACTGTGCCAG GCAGCTGGCC ACACGGGCCC CCTGCACAAG
TGTGACATCT ACCAGTCCAA GGAGGCCGGG CAGCGCCTGG CGACCGCCAT GAAGCTGGGC
TTCAGTAGGC CGTGGCCGGA AGCCATGCAG CTGATCACGG GCCAGCCCAA CATGAGCGCC
TCGGCCATGT TGAGCTACTT CAAGCCGCTG CTGGACTGGC TCCGCACGGA GAACGAGCTG
CATGGGGAGA AGCTGGGCTG GCCGCAGTAC AACTGGACGC CGAACTCCGC TCGCTCAGAA
GGGCCCCTCC CAGACAGCGG CCGCGTCAGC TTCCTGGGCC TGGACCTGGA TGCGCAGCAG
GCCCGCGTGG GCCAGTGGCT GCTGCTCTTC CTGGGCATCG CCCTGCTGGT AGCCACCCTG
GGCCTCAGCC AGCGGCTCTT CAGCATCCGC CACCGCAGCC TCCACCGGCA CTCCCACGGG
CCCCAGTTCG GCTCCGAGGT GGAGCTGAGA CACTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14342
Clone ID Related Accession (Same CDS sequence) NM_001178057
Accession Version NM_001178057.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2076bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product angiotensin-converting enzyme isoform 3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM908222.1, M26657.1, AB208971.1, AK301988.1 and AC113554.9. Transcript Variant: This variant (3) lacks multiple 5' exons and an in-frame exon in the 3' region, but has an alternate 5' exon, compared to variant 1. The resulting isoform (3) is shorter, and has a distinct N-terminus and lacks an internal segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK301988.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2151119 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGGCCAGG GTTGGGCTAC TGCAGGACTT CCCAGCCTCC TCTTCCTGCT GCTCTGCTAC 
GGGCACCCTC TGCTGGTCCC CAGCCAGGAG GCATCCCAAC AGGTGACAGT CACCCATGGG
ACAAGCAGCC AGGCAACAAC CAGCAGCCAG ACAACCACCC ACCAGGCGAC GGCCCACCAG
ACATCAGCCC AGAGCCCAAA CCTGGTGACT GATGAGGCTG AGGCCAGCAA GTTTGTGGAG
GAATATGACC GGACATCCCA GGTGGTGTGG AACGAGTATG CCGAGGCCAA CTGGAACTAC
AACACCAACA TCACCACAGA GACCAGCAAG ATTCTGCTGC AGAAGAACAT GCAAATAGCC
AACCACACCC TGAAGTACGG CACCCAGGCC AGGAAGTTTG ATGTGAACCA GTTGCAGAAC
ACCACTATCA AGCGGATCAT AAAGAAGGTT CAGGACCTAG AACGGGCAGC ACTGCCTGCC
CAGGAGCTGG AGGAGTACAA CAAGATCCTG TTGGATATGG AAACCACCTA CAGCGTGGCC
ACTGTGTGCC ACCCGAATGG CAGCTGCCTG CAGCTCGAGC CAGATCTGAC GAATGTGATG
GCCACGTCCC GGAAATATGA AGACCTGTTA TGGGCATGGG AGGGCTGGCG AGACAAGGCG
GGGAGAGCCA TCCTCCAGTT TTACCCGAAA TACGTGGAAC TCATCAACCA GGCTGCCCGG
CTCAATGGCT ATGTAGATGC AGGGGACTCG TGGAGGTCTA TGTACGAGAC ACCATCCCTG
GAGCAAGACC TGGAGCGGCT CTTCCAGGAG CTGCAGCCAC TCTACCTCAA CCTGCATGCC
TACGTGCGCC GGGCCCTGCA CCGTCACTAC GGGGCCCAGC ACATCAACCT GGAGGGGCCC
ATTCCTGCTC ACCTGCTGGG GAACATGTGG GCGCAGACCT GGTCCAACAT CTATGACTTG
GTGGTGCCCT TCCCTTCAGC CCCCTCGATG GACACCACAG AGGCTATGCT AAAGCAGGGC
TGGACGCCCA GGAGGATGTT TAAGGAGGCT GATGATTTCT TCACCTCCCT GGGGCTGCTG
CCCGTGCCTC CTGAGTTCTG GAACAAGTCG ATGCTGGAGA AGCCAACCGA CGGGCGGGAG
GTGGTCTGCC ACGCCTCGGC CTGGGACTTC TACAACGGCA AGGACTTCCG GATCAAGCAG
TGCACCACCG TGAACTTGGA GGACCTGGTG GTGGCCCACC ACGAAATGGG CCACATCCAG
TATTTCATGC AGTACAAAGA CTTACCTGTG GCCTTGAGGG AGGGTGCCAA CCCCGGCTTC
CATGAGGCCA TTGGGGACGT GCTAGCCCTC TCAGTGTCTA CGCCCAAGCA CCTGCACAGT
CTCAACCTGC TGAGCAGTGA GGGTGGCAGC GACGAGCATG ACATCAACTT TCTGATGAAG
ATGGCCCTTG ACAAGATCGC CTTTATCCCC TTCAGCTACC TCGTCGATCA GTGGCGCTGG
AGGGTATTTG ATGGAAGCAT CACCAAGGAG AACTATAACC AGGAGTGGTG GAGCCTCAGG
CTGAAGTACC AGGGCCTCTG CCCCCCAGTG CCCAGGACTC AAGGTGACTT TGACCCAGGG
GCCAAGTTCC ACATTCCTTC TAGCGTGCCT TACATCAGGA CCGCCATGAA GCTGGGCTTC
AGTAGGCCGT GGCCGGAAGC CATGCAGCTG ATCACGGGCC AGCCCAACAT GAGCGCCTCG
GCCATGTTGA GCTACTTCAA GCCGCTGCTG GACTGGCTCC GCACGGAGAA CGAGCTGCAT
GGGGAGAAGC TGGGCTGGCC GCAGTACAAC TGGACGCCGA ACTCCGCTCG CTCAGAAGGG
CCCCTCCCAG ACAGCGGCCG CGTCAGCTTC CTGGGCCTGG ACCTGGATGC GCAGCAGGCC
CGCGTGGGCC AGTGGCTGCT GCTCTTCCTG GGCATCGCCC TGCTGGTAGC CACCCTGGGC
CTCAGCCAGC GGCTCTTCAG CATCCGCCAC CGCAGCCTCC ACCGGCACTC CCACGGGCCC
CAGTTCGGCT CCGAGGTGGA GCTGAGACAC TCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001171528.1
CDS52..2127
Misc Feature(1)283..1842(+)
Misc Feature(2)1201..1704(+)
Misc Feature(3)1291..1377(+)
Exon (1)1..250
Gene:ACE
Gene Synonym:
Exon (2)251..387
Gene:ACE
Gene Synonym:
Exon (3)388..546
Gene:ACE
Gene Synonym:
Exon (4)547..634
Gene:ACE
Gene Synonym:
Exon (5)635..778
Gene:ACE
Gene Synonym:
Exon (6)779..970
Gene:ACE
Gene Synonym:
Exon (7)971..1068
Gene:ACE
Gene Synonym:
Exon (8)1069..1241
Gene:ACE
Gene Synonym:
Exon (9)1242..1465
Gene:ACE
Gene Synonym:
Exon (10)1466..1610
Gene:ACE
Gene Synonym:
Exon (11)1611..1709
Gene:ACE
Gene Synonym:
Exon (12)1710..1897
Gene:ACE
Gene Synonym:
Exon (13)1898..3141
Gene:ACE
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001178057
Organism Homo sapiens (human)
Definition Homo sapiens angiotensin I converting enzyme (ACE), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001178057

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGGCCAGG GTTGGGCTAC TGCAGGACTT CCCAGCCTCC TCTTCCTGCT GCTCTGCTAC 
GGGCACCCTC TGCTGGTCCC CAGCCAGGAG GCATCCCAAC AGGTGACAGT CACCCATGGG
ACAAGCAGCC AGGCAACAAC CAGCAGCCAG ACAACCACCC ACCAGGCGAC GGCCCACCAG
ACATCAGCCC AGAGCCCAAA CCTGGTGACT GATGAGGCTG AGGCCAGCAA GTTTGTGGAG
GAATATGACC GGACATCCCA GGTGGTGTGG AACGAGTATG CCGAGGCCAA CTGGAACTAC
AACACCAACA TCACCACAGA GACCAGCAAG ATTCTGCTGC AGAAGAACAT GCAAATAGCC
AACCACACCC TGAAGTACGG CACCCAGGCC AGGAAGTTTG ATGTGAACCA GTTGCAGAAC
ACCACTATCA AGCGGATCAT AAAGAAGGTT CAGGACCTAG AACGGGCAGC ACTGCCTGCC
CAGGAGCTGG AGGAGTACAA CAAGATCCTG TTGGATATGG AAACCACCTA CAGCGTGGCC
ACTGTGTGCC ACCCGAATGG CAGCTGCCTG CAGCTCGAGC CAGATCTGAC GAATGTGATG
GCCACGTCCC GGAAATATGA AGACCTGTTA TGGGCATGGG AGGGCTGGCG AGACAAGGCG
GGGAGAGCCA TCCTCCAGTT TTACCCGAAA TACGTGGAAC TCATCAACCA GGCTGCCCGG
CTCAATGGCT ATGTAGATGC AGGGGACTCG TGGAGGTCTA TGTACGAGAC ACCATCCCTG
GAGCAAGACC TGGAGCGGCT CTTCCAGGAG CTGCAGCCAC TCTACCTCAA CCTGCATGCC
TACGTGCGCC GGGCCCTGCA CCGTCACTAC GGGGCCCAGC ACATCAACCT GGAGGGGCCC
ATTCCTGCTC ACCTGCTGGG GAACATGTGG GCGCAGACCT GGTCCAACAT CTATGACTTG
GTGGTGCCCT TCCCTTCAGC CCCCTCGATG GACACCACAG AGGCTATGCT AAAGCAGGGC
TGGACGCCCA GGAGGATGTT TAAGGAGGCT GATGATTTCT TCACCTCCCT GGGGCTGCTG
CCCGTGCCTC CTGAGTTCTG GAACAAGTCG ATGCTGGAGA AGCCAACCGA CGGGCGGGAG
GTGGTCTGCC ACGCCTCGGC CTGGGACTTC TACAACGGCA AGGACTTCCG GATCAAGCAG
TGCACCACCG TGAACTTGGA GGACCTGGTG GTGGCCCACC ACGAAATGGG CCACATCCAG
TATTTCATGC AGTACAAAGA CTTACCTGTG GCCTTGAGGG AGGGTGCCAA CCCCGGCTTC
CATGAGGCCA TTGGGGACGT GCTAGCCCTC TCAGTGTCTA CGCCCAAGCA CCTGCACAGT
CTCAACCTGC TGAGCAGTGA GGGTGGCAGC GACGAGCATG ACATCAACTT TCTGATGAAG
ATGGCCCTTG ACAAGATCGC CTTTATCCCC TTCAGCTACC TCGTCGATCA GTGGCGCTGG
AGGGTATTTG ATGGAAGCAT CACCAAGGAG AACTATAACC AGGAGTGGTG GAGCCTCAGG
CTGAAGTACC AGGGCCTCTG CCCCCCAGTG CCCAGGACTC AAGGTGACTT TGACCCAGGG
GCCAAGTTCC ACATTCCTTC TAGCGTGCCT TACATCAGGA CCGCCATGAA GCTGGGCTTC
AGTAGGCCGT GGCCGGAAGC CATGCAGCTG ATCACGGGCC AGCCCAACAT GAGCGCCTCG
GCCATGTTGA GCTACTTCAA GCCGCTGCTG GACTGGCTCC GCACGGAGAA CGAGCTGCAT
GGGGAGAAGC TGGGCTGGCC GCAGTACAAC TGGACGCCGA ACTCCGCTCG CTCAGAAGGG
CCCCTCCCAG ACAGCGGCCG CGTCAGCTTC CTGGGCCTGG ACCTGGATGC GCAGCAGGCC
CGCGTGGGCC AGTGGCTGCT GCTCTTCCTG GGCATCGCCC TGCTGGTAGC CACCCTGGGC
CTCAGCCAGC GGCTCTTCAG CATCCGCCAC CGCAGCCTCC ACCGGCACTC CCACGGGCCC
CAGTTCGGCT CCGAGGTGGA GCTGAGACAC TCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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