HFM1 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following HFM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HFM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu23632 NM_001017975.5
Latest version!
Homo sapiens helicase for meiosis 1 (HFM1), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$659.50-$923.30
$1319.00
OHu77233 XM_011540851.1
Latest version!
Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$519.50-$727.30
$1039.00
OHu77233 XM_011540850.2
Latest version!
Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$519.50-$727.30
$1039.00
OHu77233 XM_011540849.1
Latest version!
Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$519.50-$727.30
$1039.00
OHu77233 XM_011540852.2
Latest version!
Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$519.50-$727.30
$1039.00
OHu77241 XM_017000496.1
Latest version!
Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu77241 XM_017000495.1
Latest version!
Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu103608 XM_024453708.1
Latest version!
Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu83247 XM_017000493.1
Latest version!
Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu83248 XM_017000494.1
Latest version!
Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu77239 XM_017000492.1
Latest version!
Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $559.30
$799.00
OHu77240 XM_011540859.2
Latest version!
Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $559.30
$799.00
OHu77238 XM_011540857.1
Latest version!
Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu77232 XM_017000490.1
Latest version!
Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu77234 XM_017000491.1
Latest version!
Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu77236 XM_011540855.2
Latest version!
Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
View Old Accession Versions >>
OHu77236 XM_011540855.1 Homo sapiens HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu23632 NM_001017975.4 Homo sapiens HFM1, ATP dependent DNA helicase homolog (HFM1), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$659.50-$923.30
$1319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu23632
    Clone ID Related Accession (Same CDS sequence) NM_001017975.4 , NM_001017975.5
    Accession Version NM_001017975.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-02
    Organism Homo sapiens(human)
    Product probable ATP-dependent DNA helicase HFM1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB204867.1, AC098691.2 and BX323048.4. This sequence is a reference standard in the RefSeqGene project. On Apr 22, 2014 this sequence version replaced NM_001017975.3. ##Evidence-Data-START## Transcript exon combination :: AB204867.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA ACCATCCAGA CAATGAAAAG TCATTGGATT GGTTTCTCCC TCCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGAG TTAGAGGAAG AATTAGAAAG TCATAAACTG
    TTAGGTCAGG AAAAGAGGCC AAAAATGTTA ACATCAAATT TAAAGATAAC TAATGAAGAT
    ACAAATTATA TTTCACTAAC ACAAAAATTC CAGTTTGCCT TTCCTTCTGA TAAATATGAA
    CAGGATGATC TAAATTTAGA AGGGGTAGGT AATAATGACT TATCACATGT TGCTGGCAAG
    CTGACATATG CTTCTCAGAA ATATAAAAAT CACATTGGCA CTGAGATAGC ACCTGAGAAG
    AGTGTTCCTG ATGATACAAA ATTAGTTAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GTATTCCGGA AAAGATTATT TAAAATATCT GACAATATAC ATGGGAGTGC TTATTCTAAT
    GACAATGAAT TGGACTCTCA CATTGGCTCA GTGAAAATTG TACAAACAGA AATGAACAAA
    GGGAAATCAA GGAACTATAG CAATAGTAAG CAGAAATTTC AGTATTCTGC AAATGTGTTT
    ACAGCAAATA ATGCTTTTTC TGCTTCTGAA ATCGGAGAAG GCATGTTCAA AGCACCATCT
    TTTTCAGTTG CTTTCCAACC TCATGATATT CAAGAGGTAA CAGAAAATGG TTTAGGTTCC
    TTGAAGGCTG TCACAGAAAT TCCGGCAAAA TTTAGAAGTA TTTTCAAAGA ATTTCCATAT
    TTCAACTATA TACAGTCCAA GGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGAAAAACT GTAGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTTGAAT ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCTTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TTACTGGAGA TACAGTAATG GATGATCTAT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTTCAGC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATATTGTAAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TGTTTCTCAG
    ACTTTAAAAA ATACCAGCAC TGCTATTCCA ATGCGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGACCAGC TGTGTGTCTG
    AAAATGGATG AGAGCCATAG ACCAGTGAAA CTTCAGAAAG TGGTCCTTGG ATTTCCCTGC
    AGTAGTAACC AAACTGAGTT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    ATACAAATGT ACTCTGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAGGGTGTG
    CAACAGGCTG CTTCTGTTCT TGTGAAAGAT GCTAAATTTA TTATGACTGT GGAACAGAAA
    CAGAGGTTAC AGAAGTATGC ATATTCCGTA AGAGATTCAA AACTGAGAGA TATCTTAAAA
    GATGGTGCTG CTTATCATCA TGCTGGTATG GAGCTGTCAG ATAGAAAAGT AGTTGAGGGA
    GCTTTTACTG TTGGAGATTT ACCAGTTCTT TTTACTACCA GTACTTTAGC TATGGGAGTA
    AATTTGCCTG CTCACCTAGT AGTTATAAAA TCTACAATGC ATTATGCTGG AGGACTGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CTACTGCAGT TATCATGACT CGATTAAGCA CAAGGGACAA GTACATTCAG
    ATGTTAGCTT GTAGAGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAACATTTA
    AATGCAGAGA TAGTACTGCA TACCATCACG GATGTGAATA TTGCTGTGGA ATGGATACGA
    TCAACTCTGC TTTATATCAG AGCCTTGAAA AATCCATCTC ATTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA AGCAAAATTA CAAGAATTAT GTTTGAAGAA TCTGAATGAT
    TTATCATCCC TGGACTTAAT AAAGATGGAT GAAGGTGTTA ATTTCAAACC AACTGAAGCA
    GGAAGATTGA TGGCTTGGTA TTATATTACA TTTGAGACAG TGAAGAAATT TTATACAATC
    AGTGGAAAAG AAACCTTATC AGATCTGGTT ACATTGATAG CTGGCTGCAA GGAATTTCTA
    GATATACAGT TAAGGATAAA TGAAAAGAAA ACACTGAATA CTTTGAACAA AGATCCAAAT
    CGGATAACTA TCAGATTTCC AATGGAAGGA AGAATTAAAA CAAGAGAAAT GAAAGTAAAT
    TGTCTTATTC AGGCTCAACT AGGATGCATT CCCATACAAG ATTTTGCTTT GACACAAGAT
    ACCGCAAAGA TTTTCAGACA TGGCTCCCGA ATTACAAGAT GGTTGTCAGA TTTTGTAGCT
    GCTCAAGAAA AGAAGTTTGC TGTACTATTG AATAGTTTGA TTTTAGCTAA ATGTTTTAGG
    TGTAAACTTT GGGAAAACTC TCTGCATGTA TCCAAACAAC TGGAAAAAAT TGGTATAACA
    TTGTCAAATG CAATCGTAAA TGCTGGTTTG ACTTCCTTTA AAAAAATAGA AGAGACAGAT
    GCAAGGGAAC TTGAATTGAT TTTAAACAGA CATCCCCCCT TTGGAACCCA GATAAAAGAA
    ACTGTGATGT ATCTACCAAA ATATGAACTT AAAGTGGAAC AGATTACAAG ATATAGTGAT
    ACGACGGCAG AAATATTAGT GACTGTTATA TTAAGAAATT TTGAACAGCT ACAAACTAAA
    AGAACAGCAT CGGATTCTCA CTATGTTACC TTAATCATAG GTGACGCAGA TAATCAAGTA
    GTTTATCTGC ACAAGATTAC GGATTCTGTT TTGCTAAAAG CTGGAAGTTG GGCTAAAAAG
    ATTGCTGTGA AAAGAGCTCT TAAATCTGAA GATCTTAGCA TAAATCTAAT AAGTTCTGAA
    TTTGTTGGGC TTGATATTCA GCAGAAACTT ACAGTCTTTT ACTTAGAACC CAAGAGGTTT
    GGAAATCAAA TCACTATGCA AAGAAAATCT GAAACACAGA TTTCCCATTC TAAACATTCA
    GACATATCTA CAATAGCAGG ACCTAATAAA GGAACGACTG CCAGCAAAAA ACCTGGGAAC
    CGAGAATGCA ATCATCTTTG TAAAAGTAAA CATACATGTG GACATGACTG CTGTAAAATT
    GGAGTTGCAC AGAAGTCAGA AATTAAAGAG TCAACAATTT CTTCATATTT ATCTGATTTA
    AGAAACAGGA ATGCTGTTTC ATCTGTTCCT CCAGTTAAAC GTCTGAAGAT ACAGATGAAT
    AAATCTCAAA GTGTGGACCT TAAAGAGTTT GGTTTTACTC CAAAACCTTC CCTTCCTAGT
    ATATCAAGGT CAGAATATTT AAACATATCT GAATTGCCTA TAATGGAGCA GTGGGATCAG
    CCTGAAATCT ATGGAAAAGT TAGACAAGAA CCATCTGAAT ATCAAGACAA AGAAGTTTTG
    AATGTGAACT TTGAATTGGG AAATGAAGTT TGGGATGATT TTGATGATGA AAACTTAGAA
    GTTACTAGCT TTTCAACTGA TACTGAGAAG ACAAAAATAT CAGGATTTGG AAACACTTTG
    AGTTCAAGTA CCAGGGGAAG TAAGCTACCC CTTCAAGAGT CAAAGAGCAA ATTCCAAAGA
    GAAATGTCAA ACAGTTTTGT TTCATCACAT GAGATGTCGG ATATTTCTTT ATCAAATTCT
    GCTATGCCCA AGTTCAGTGC ATCCTCCATG ACAAAATTAC CTCAACAAGC TGGAAATGCA
    GTTATTGTCC ATTTTCAAGA AAGAAAACCA CAAAATCTGT CACCAGAGAT TGAGAAGCAA
    TGCTTTACTT TCTCTGAAAA AAACCCAAAT TCTTCAAATT ATAAAAAAGT GGATTTTTTT
    ATTAGAAACA GTGAATGTAA AAAGGAAGTT GATTTCAGTA TGTATCATCC TGATGATGAA
    GCTGATGAAA TGAAGTCTTT ATTGGGAATA TTTGATGGTA TTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001017975.4
    CDS100..4407
    Translation

    Target ORF information:

    RefSeq Version NM_001017975.4
    Organism Homo sapiens(human)
    Definition Homo sapiens HFM1, ATP dependent DNA helicase homolog (HFM1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001017975.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA ACCATCCAGA CAATGAAAAG TCATTGGATT GGTTTCTCCC TCCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGAG TTAGAGGAAG AATTAGAAAG TCATAAACTG
    TTAGGTCAGG AAAAGAGGCC AAAAATGTTA ACATCAAATT TAAAGATAAC TAATGAAGAT
    ACAAATTATA TTTCACTAAC ACAAAAATTC CAGTTTGCCT TTCCTTCTGA TAAATATGAA
    CAGGATGATC TAAATTTAGA AGGGGTAGGT AATAATGACT TATCACATGT TGCTGGCAAG
    CTGACATATG CTTCTCAGAA ATATAAAAAT CACATTGGCA CTGAGATAGC ACCTGAGAAG
    AGTGTTCCTG ATGATACAAA ATTAGTTAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GTATTCCGGA AAAGATTATT TAAAATATCT GACAATATAC ATGGGAGTGC TTATTCTAAT
    GACAATGAAT TGGACTCTCA CATTGGCTCA GTGAAAATTG TACAAACAGA AATGAACAAA
    GGGAAATCAA GGAACTATAG CAATAGTAAG CAGAAATTTC AGTATTCTGC AAATGTGTTT
    ACAGCAAATA ATGCTTTTTC TGCTTCTGAA ATCGGAGAAG GCATGTTCAA AGCACCATCT
    TTTTCAGTTG CTTTCCAACC TCATGATATT CAAGAGGTAA CAGAAAATGG TTTAGGTTCC
    TTGAAGGCTG TCACAGAAAT TCCGGCAAAA TTTAGAAGTA TTTTCAAAGA ATTTCCATAT
    TTCAACTATA TACAGTCCAA GGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGAAAAACT GTAGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTTGAAT ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCTTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TTACTGGAGA TACAGTAATG GATGATCTAT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTTCAGC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATATTGTAAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TGTTTCTCAG
    ACTTTAAAAA ATACCAGCAC TGCTATTCCA ATGCGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGACCAGC TGTGTGTCTG
    AAAATGGATG AGAGCCATAG ACCAGTGAAA CTTCAGAAAG TGGTCCTTGG ATTTCCCTGC
    AGTAGTAACC AAACTGAGTT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    ATACAAATGT ACTCTGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAGGGTGTG
    CAACAGGCTG CTTCTGTTCT TGTGAAAGAT GCTAAATTTA TTATGACTGT GGAACAGAAA
    CAGAGGTTAC AGAAGTATGC ATATTCCGTA AGAGATTCAA AACTGAGAGA TATCTTAAAA
    GATGGTGCTG CTTATCATCA TGCTGGTATG GAGCTGTCAG ATAGAAAAGT AGTTGAGGGA
    GCTTTTACTG TTGGAGATTT ACCAGTTCTT TTTACTACCA GTACTTTAGC TATGGGAGTA
    AATTTGCCTG CTCACCTAGT AGTTATAAAA TCTACAATGC ATTATGCTGG AGGACTGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CTACTGCAGT TATCATGACT CGATTAAGCA CAAGGGACAA GTACATTCAG
    ATGTTAGCTT GTAGAGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAACATTTA
    AATGCAGAGA TAGTACTGCA TACCATCACG GATGTGAATA TTGCTGTGGA ATGGATACGA
    TCAACTCTGC TTTATATCAG AGCCTTGAAA AATCCATCTC ATTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA AGCAAAATTA CAAGAATTAT GTTTGAAGAA TCTGAATGAT
    TTATCATCCC TGGACTTAAT AAAGATGGAT GAAGGTGTTA ATTTCAAACC AACTGAAGCA
    GGAAGATTGA TGGCTTGGTA TTATATTACA TTTGAGACAG TGAAGAAATT TTATACAATC
    AGTGGAAAAG AAACCTTATC AGATCTGGTT ACATTGATAG CTGGCTGCAA GGAATTTCTA
    GATATACAGT TAAGGATAAA TGAAAAGAAA ACACTGAATA CTTTGAACAA AGATCCAAAT
    CGGATAACTA TCAGATTTCC AATGGAAGGA AGAATTAAAA CAAGAGAAAT GAAAGTAAAT
    TGTCTTATTC AGGCTCAACT AGGATGCATT CCCATACAAG ATTTTGCTTT GACACAAGAT
    ACCGCAAAGA TTTTCAGACA TGGCTCCCGA ATTACAAGAT GGTTGTCAGA TTTTGTAGCT
    GCTCAAGAAA AGAAGTTTGC TGTACTATTG AATAGTTTGA TTTTAGCTAA ATGTTTTAGG
    TGTAAACTTT GGGAAAACTC TCTGCATGTA TCCAAACAAC TGGAAAAAAT TGGTATAACA
    TTGTCAAATG CAATCGTAAA TGCTGGTTTG ACTTCCTTTA AAAAAATAGA AGAGACAGAT
    GCAAGGGAAC TTGAATTGAT TTTAAACAGA CATCCCCCCT TTGGAACCCA GATAAAAGAA
    ACTGTGATGT ATCTACCAAA ATATGAACTT AAAGTGGAAC AGATTACAAG ATATAGTGAT
    ACGACGGCAG AAATATTAGT GACTGTTATA TTAAGAAATT TTGAACAGCT ACAAACTAAA
    AGAACAGCAT CGGATTCTCA CTATGTTACC TTAATCATAG GTGACGCAGA TAATCAAGTA
    GTTTATCTGC ACAAGATTAC GGATTCTGTT TTGCTAAAAG CTGGAAGTTG GGCTAAAAAG
    ATTGCTGTGA AAAGAGCTCT TAAATCTGAA GATCTTAGCA TAAATCTAAT AAGTTCTGAA
    TTTGTTGGGC TTGATATTCA GCAGAAACTT ACAGTCTTTT ACTTAGAACC CAAGAGGTTT
    GGAAATCAAA TCACTATGCA AAGAAAATCT GAAACACAGA TTTCCCATTC TAAACATTCA
    GACATATCTA CAATAGCAGG ACCTAATAAA GGAACGACTG CCAGCAAAAA ACCTGGGAAC
    CGAGAATGCA ATCATCTTTG TAAAAGTAAA CATACATGTG GACATGACTG CTGTAAAATT
    GGAGTTGCAC AGAAGTCAGA AATTAAAGAG TCAACAATTT CTTCATATTT ATCTGATTTA
    AGAAACAGGA ATGCTGTTTC ATCTGTTCCT CCAGTTAAAC GTCTGAAGAT ACAGATGAAT
    AAATCTCAAA GTGTGGACCT TAAAGAGTTT GGTTTTACTC CAAAACCTTC CCTTCCTAGT
    ATATCAAGGT CAGAATATTT AAACATATCT GAATTGCCTA TAATGGAGCA GTGGGATCAG
    CCTGAAATCT ATGGAAAAGT TAGACAAGAA CCATCTGAAT ATCAAGACAA AGAAGTTTTG
    AATGTGAACT TTGAATTGGG AAATGAAGTT TGGGATGATT TTGATGATGA AAACTTAGAA
    GTTACTAGCT TTTCAACTGA TACTGAGAAG ACAAAAATAT CAGGATTTGG AAACACTTTG
    AGTTCAAGTA CCAGGGGAAG TAAGCTACCC CTTCAAGAGT CAAAGAGCAA ATTCCAAAGA
    GAAATGTCAA ACAGTTTTGT TTCATCACAT GAGATGTCGG ATATTTCTTT ATCAAATTCT
    GCTATGCCCA AGTTCAGTGC ATCCTCCATG ACAAAATTAC CTCAACAAGC TGGAAATGCA
    GTTATTGTCC ATTTTCAAGA AAGAAAACCA CAAAATCTGT CACCAGAGAT TGAGAAGCAA
    TGCTTTACTT TCTCTGAAAA AAACCCAAAT TCTTCAAATT ATAAAAAAGT GGATTTTTTT
    ATTAGAAACA GTGAATGTAA AAAGGAAGTT GATTTCAGTA TGTATCATCC TGATGATGAA
    GCTGATGAAA TGAAGTCTTT ATTGGGAATA TTTGATGGTA TTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23632
    Clone ID Related Accession (Same CDS sequence) NM_001017975.4 , NM_001017975.5
    Accession Version NM_001017975.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-29
    Organism Homo sapiens(human)
    Product probable ATP-dependent DNA helicase HFM1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB204867.1, AC098691.2 and BX323048.4. On Nov 22, 2018 this sequence version replaced NM_001017975.4. ##Evidence-Data-START## Transcript exon combination :: AB204867.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA ACCATCCAGA CAATGAAAAG TCATTGGATT GGTTTCTCCC TCCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGAG TTAGAGGAAG AATTAGAAAG TCATAAACTG
    TTAGGTCAGG AAAAGAGGCC AAAAATGTTA ACATCAAATT TAAAGATAAC TAATGAAGAT
    ACAAATTATA TTTCACTAAC ACAAAAATTC CAGTTTGCCT TTCCTTCTGA TAAATATGAA
    CAGGATGATC TAAATTTAGA AGGGGTAGGT AATAATGACT TATCACATGT TGCTGGCAAG
    CTGACATATG CTTCTCAGAA ATATAAAAAT CACATTGGCA CTGAGATAGC ACCTGAGAAG
    AGTGTTCCTG ATGATACAAA ATTAGTTAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GTATTCCGGA AAAGATTATT TAAAATATCT GACAATATAC ATGGGAGTGC TTATTCTAAT
    GACAATGAAT TGGACTCTCA CATTGGCTCA GTGAAAATTG TACAAACAGA AATGAACAAA
    GGGAAATCAA GGAACTATAG CAATAGTAAG CAGAAATTTC AGTATTCTGC AAATGTGTTT
    ACAGCAAATA ATGCTTTTTC TGCTTCTGAA ATCGGAGAAG GCATGTTCAA AGCACCATCT
    TTTTCAGTTG CTTTCCAACC TCATGATATT CAAGAGGTAA CAGAAAATGG TTTAGGTTCC
    TTGAAGGCTG TCACAGAAAT TCCGGCAAAA TTTAGAAGTA TTTTCAAAGA ATTTCCATAT
    TTCAACTATA TACAGTCCAA GGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGAAAAACT GTAGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTTGAAT ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCTTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TTACTGGAGA TACAGTAATG GATGATCTAT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTTCAGC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATATTGTAAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TGTTTCTCAG
    ACTTTAAAAA ATACCAGCAC TGCTATTCCA ATGCGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGACCAGC TGTGTGTCTG
    AAAATGGATG AGAGCCATAG ACCAGTGAAA CTTCAGAAAG TGGTCCTTGG ATTTCCCTGC
    AGTAGTAACC AAACTGAGTT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    ATACAAATGT ACTCTGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAGGGTGTG
    CAACAGGCTG CTTCTGTTCT TGTGAAAGAT GCTAAATTTA TTATGACTGT GGAACAGAAA
    CAGAGGTTAC AGAAGTATGC ATATTCCGTA AGAGATTCAA AACTGAGAGA TATCTTAAAA
    GATGGTGCTG CTTATCATCA TGCTGGTATG GAGCTGTCAG ATAGAAAAGT AGTTGAGGGA
    GCTTTTACTG TTGGAGATTT ACCAGTTCTT TTTACTACCA GTACTTTAGC TATGGGAGTA
    AATTTGCCTG CTCACCTAGT AGTTATAAAA TCTACAATGC ATTATGCTGG AGGACTGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CTACTGCAGT TATCATGACT CGATTAAGCA CAAGGGACAA GTACATTCAG
    ATGTTAGCTT GTAGAGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAACATTTA
    AATGCAGAGA TAGTACTGCA TACCATCACG GATGTGAATA TTGCTGTGGA ATGGATACGA
    TCAACTCTGC TTTATATCAG AGCCTTGAAA AATCCATCTC ATTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA AGCAAAATTA CAAGAATTAT GTTTGAAGAA TCTGAATGAT
    TTATCATCCC TGGACTTAAT AAAGATGGAT GAAGGTGTTA ATTTCAAACC AACTGAAGCA
    GGAAGATTGA TGGCTTGGTA TTATATTACA TTTGAGACAG TGAAGAAATT TTATACAATC
    AGTGGAAAAG AAACCTTATC AGATCTGGTT ACATTGATAG CTGGCTGCAA GGAATTTCTA
    GATATACAGT TAAGGATAAA TGAAAAGAAA ACACTGAATA CTTTGAACAA AGATCCAAAT
    CGGATAACTA TCAGATTTCC AATGGAAGGA AGAATTAAAA CAAGAGAAAT GAAAGTAAAT
    TGTCTTATTC AGGCTCAACT AGGATGCATT CCCATACAAG ATTTTGCTTT GACACAAGAT
    ACCGCAAAGA TTTTCAGACA TGGCTCCCGA ATTACAAGAT GGTTGTCAGA TTTTGTAGCT
    GCTCAAGAAA AGAAGTTTGC TGTACTATTG AATAGTTTGA TTTTAGCTAA ATGTTTTAGG
    TGTAAACTTT GGGAAAACTC TCTGCATGTA TCCAAACAAC TGGAAAAAAT TGGTATAACA
    TTGTCAAATG CAATCGTAAA TGCTGGTTTG ACTTCCTTTA AAAAAATAGA AGAGACAGAT
    GCAAGGGAAC TTGAATTGAT TTTAAACAGA CATCCCCCCT TTGGAACCCA GATAAAAGAA
    ACTGTGATGT ATCTACCAAA ATATGAACTT AAAGTGGAAC AGATTACAAG ATATAGTGAT
    ACGACGGCAG AAATATTAGT GACTGTTATA TTAAGAAATT TTGAACAGCT ACAAACTAAA
    AGAACAGCAT CGGATTCTCA CTATGTTACC TTAATCATAG GTGACGCAGA TAATCAAGTA
    GTTTATCTGC ACAAGATTAC GGATTCTGTT TTGCTAAAAG CTGGAAGTTG GGCTAAAAAG
    ATTGCTGTGA AAAGAGCTCT TAAATCTGAA GATCTTAGCA TAAATCTAAT AAGTTCTGAA
    TTTGTTGGGC TTGATATTCA GCAGAAACTT ACAGTCTTTT ACTTAGAACC CAAGAGGTTT
    GGAAATCAAA TCACTATGCA AAGAAAATCT GAAACACAGA TTTCCCATTC TAAACATTCA
    GACATATCTA CAATAGCAGG ACCTAATAAA GGAACGACTG CCAGCAAAAA ACCTGGGAAC
    CGAGAATGCA ATCATCTTTG TAAAAGTAAA CATACATGTG GACATGACTG CTGTAAAATT
    GGAGTTGCAC AGAAGTCAGA AATTAAAGAG TCAACAATTT CTTCATATTT ATCTGATTTA
    AGAAACAGGA ATGCTGTTTC ATCTGTTCCT CCAGTTAAAC GTCTGAAGAT ACAGATGAAT
    AAATCTCAAA GTGTGGACCT TAAAGAGTTT GGTTTTACTC CAAAACCTTC CCTTCCTAGT
    ATATCAAGGT CAGAATATTT AAACATATCT GAATTGCCTA TAATGGAGCA GTGGGATCAG
    CCTGAAATCT ATGGAAAAGT TAGACAAGAA CCATCTGAAT ATCAAGACAA AGAAGTTTTG
    AATGTGAACT TTGAATTGGG AAATGAAGTT TGGGATGATT TTGATGATGA AAACTTAGAA
    GTTACTAGCT TTTCAACTGA TACTGAGAAG ACAAAAATAT CAGGATTTGG AAACACTTTG
    AGTTCAAGTA CCAGGGGAAG TAAGCTACCC CTTCAAGAGT CAAAGAGCAA ATTCCAAAGA
    GAAATGTCAA ACAGTTTTGT TTCATCACAT GAGATGTCGG ATATTTCTTT ATCAAATTCT
    GCTATGCCCA AGTTCAGTGC ATCCTCCATG ACAAAATTAC CTCAACAAGC TGGAAATGCA
    GTTATTGTCC ATTTTCAAGA AAGAAAACCA CAAAATCTGT CACCAGAGAT TGAGAAGCAA
    TGCTTTACTT TCTCTGAAAA AAACCCAAAT TCTTCAAATT ATAAAAAAGT GGATTTTTTT
    ATTAGAAACA GTGAATGTAA AAAGGAAGTT GATTTCAGTA TGTATCATCC TGATGATGAA
    GCTGATGAAA TGAAGTCTTT ATTGGGAATA TTTGATGGTA TTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001017975.4
    CDS68..4375
    Translation

    Target ORF information:

    RefSeq Version NM_001017975.5
    Organism Homo sapiens(human)
    Definition Homo sapiens helicase for meiosis 1 (HFM1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001017975.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA ACCATCCAGA CAATGAAAAG TCATTGGATT GGTTTCTCCC TCCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGAG TTAGAGGAAG AATTAGAAAG TCATAAACTG
    TTAGGTCAGG AAAAGAGGCC AAAAATGTTA ACATCAAATT TAAAGATAAC TAATGAAGAT
    ACAAATTATA TTTCACTAAC ACAAAAATTC CAGTTTGCCT TTCCTTCTGA TAAATATGAA
    CAGGATGATC TAAATTTAGA AGGGGTAGGT AATAATGACT TATCACATGT TGCTGGCAAG
    CTGACATATG CTTCTCAGAA ATATAAAAAT CACATTGGCA CTGAGATAGC ACCTGAGAAG
    AGTGTTCCTG ATGATACAAA ATTAGTTAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GTATTCCGGA AAAGATTATT TAAAATATCT GACAATATAC ATGGGAGTGC TTATTCTAAT
    GACAATGAAT TGGACTCTCA CATTGGCTCA GTGAAAATTG TACAAACAGA AATGAACAAA
    GGGAAATCAA GGAACTATAG CAATAGTAAG CAGAAATTTC AGTATTCTGC AAATGTGTTT
    ACAGCAAATA ATGCTTTTTC TGCTTCTGAA ATCGGAGAAG GCATGTTCAA AGCACCATCT
    TTTTCAGTTG CTTTCCAACC TCATGATATT CAAGAGGTAA CAGAAAATGG TTTAGGTTCC
    TTGAAGGCTG TCACAGAAAT TCCGGCAAAA TTTAGAAGTA TTTTCAAAGA ATTTCCATAT
    TTCAACTATA TACAGTCCAA GGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGAAAAACT GTAGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTTGAAT ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCTTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TTACTGGAGA TACAGTAATG GATGATCTAT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTTCAGC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATATTGTAAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TGTTTCTCAG
    ACTTTAAAAA ATACCAGCAC TGCTATTCCA ATGCGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGACCAGC TGTGTGTCTG
    AAAATGGATG AGAGCCATAG ACCAGTGAAA CTTCAGAAAG TGGTCCTTGG ATTTCCCTGC
    AGTAGTAACC AAACTGAGTT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    ATACAAATGT ACTCTGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAGGGTGTG
    CAACAGGCTG CTTCTGTTCT TGTGAAAGAT GCTAAATTTA TTATGACTGT GGAACAGAAA
    CAGAGGTTAC AGAAGTATGC ATATTCCGTA AGAGATTCAA AACTGAGAGA TATCTTAAAA
    GATGGTGCTG CTTATCATCA TGCTGGTATG GAGCTGTCAG ATAGAAAAGT AGTTGAGGGA
    GCTTTTACTG TTGGAGATTT ACCAGTTCTT TTTACTACCA GTACTTTAGC TATGGGAGTA
    AATTTGCCTG CTCACCTAGT AGTTATAAAA TCTACAATGC ATTATGCTGG AGGACTGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CTACTGCAGT TATCATGACT CGATTAAGCA CAAGGGACAA GTACATTCAG
    ATGTTAGCTT GTAGAGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAACATTTA
    AATGCAGAGA TAGTACTGCA TACCATCACG GATGTGAATA TTGCTGTGGA ATGGATACGA
    TCAACTCTGC TTTATATCAG AGCCTTGAAA AATCCATCTC ATTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA AGCAAAATTA CAAGAATTAT GTTTGAAGAA TCTGAATGAT
    TTATCATCCC TGGACTTAAT AAAGATGGAT GAAGGTGTTA ATTTCAAACC AACTGAAGCA
    GGAAGATTGA TGGCTTGGTA TTATATTACA TTTGAGACAG TGAAGAAATT TTATACAATC
    AGTGGAAAAG AAACCTTATC AGATCTGGTT ACATTGATAG CTGGCTGCAA GGAATTTCTA
    GATATACAGT TAAGGATAAA TGAAAAGAAA ACACTGAATA CTTTGAACAA AGATCCAAAT
    CGGATAACTA TCAGATTTCC AATGGAAGGA AGAATTAAAA CAAGAGAAAT GAAAGTAAAT
    TGTCTTATTC AGGCTCAACT AGGATGCATT CCCATACAAG ATTTTGCTTT GACACAAGAT
    ACCGCAAAGA TTTTCAGACA TGGCTCCCGA ATTACAAGAT GGTTGTCAGA TTTTGTAGCT
    GCTCAAGAAA AGAAGTTTGC TGTACTATTG AATAGTTTGA TTTTAGCTAA ATGTTTTAGG
    TGTAAACTTT GGGAAAACTC TCTGCATGTA TCCAAACAAC TGGAAAAAAT TGGTATAACA
    TTGTCAAATG CAATCGTAAA TGCTGGTTTG ACTTCCTTTA AAAAAATAGA AGAGACAGAT
    GCAAGGGAAC TTGAATTGAT TTTAAACAGA CATCCCCCCT TTGGAACCCA GATAAAAGAA
    ACTGTGATGT ATCTACCAAA ATATGAACTT AAAGTGGAAC AGATTACAAG ATATAGTGAT
    ACGACGGCAG AAATATTAGT GACTGTTATA TTAAGAAATT TTGAACAGCT ACAAACTAAA
    AGAACAGCAT CGGATTCTCA CTATGTTACC TTAATCATAG GTGACGCAGA TAATCAAGTA
    GTTTATCTGC ACAAGATTAC GGATTCTGTT TTGCTAAAAG CTGGAAGTTG GGCTAAAAAG
    ATTGCTGTGA AAAGAGCTCT TAAATCTGAA GATCTTAGCA TAAATCTAAT AAGTTCTGAA
    TTTGTTGGGC TTGATATTCA GCAGAAACTT ACAGTCTTTT ACTTAGAACC CAAGAGGTTT
    GGAAATCAAA TCACTATGCA AAGAAAATCT GAAACACAGA TTTCCCATTC TAAACATTCA
    GACATATCTA CAATAGCAGG ACCTAATAAA GGAACGACTG CCAGCAAAAA ACCTGGGAAC
    CGAGAATGCA ATCATCTTTG TAAAAGTAAA CATACATGTG GACATGACTG CTGTAAAATT
    GGAGTTGCAC AGAAGTCAGA AATTAAAGAG TCAACAATTT CTTCATATTT ATCTGATTTA
    AGAAACAGGA ATGCTGTTTC ATCTGTTCCT CCAGTTAAAC GTCTGAAGAT ACAGATGAAT
    AAATCTCAAA GTGTGGACCT TAAAGAGTTT GGTTTTACTC CAAAACCTTC CCTTCCTAGT
    ATATCAAGGT CAGAATATTT AAACATATCT GAATTGCCTA TAATGGAGCA GTGGGATCAG
    CCTGAAATCT ATGGAAAAGT TAGACAAGAA CCATCTGAAT ATCAAGACAA AGAAGTTTTG
    AATGTGAACT TTGAATTGGG AAATGAAGTT TGGGATGATT TTGATGATGA AAACTTAGAA
    GTTACTAGCT TTTCAACTGA TACTGAGAAG ACAAAAATAT CAGGATTTGG AAACACTTTG
    AGTTCAAGTA CCAGGGGAAG TAAGCTACCC CTTCAAGAGT CAAAGAGCAA ATTCCAAAGA
    GAAATGTCAA ACAGTTTTGT TTCATCACAT GAGATGTCGG ATATTTCTTT ATCAAATTCT
    GCTATGCCCA AGTTCAGTGC ATCCTCCATG ACAAAATTAC CTCAACAAGC TGGAAATGCA
    GTTATTGTCC ATTTTCAAGA AAGAAAACCA CAAAATCTGT CACCAGAGAT TGAGAAGCAA
    TGCTTTACTT TCTCTGAAAA AAACCCAAAT TCTTCAAATT ATAAAAAAGT GGATTTTTTT
    ATTAGAAACA GTGAATGTAA AAAGGAAGTT GATTTCAGTA TGTATCATCC TGATGATGAA
    GCTGATGAAA TGAAGTCTTT ATTGGGAATA TTTGATGGTA TTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu77233
    Clone ID Related Accession (Same CDS sequence) XM_011540849.1 , XM_011540850.2 , XM_011540851.1 , XM_011540852.2
    Accession Version XM_011540849.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA ACCATCCAGA CAATGAAAAG TCATTGGATT GGTTTCTCCC TCCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGAG TTAGAGGAAG AATTAGAAAG TCATAAACTG
    TTAGGTCAGG AAAAGAGGCC AAAAATGTTA ACATCAAATT TAAAGATAAC TAATGAAGAT
    ACAAATTATA TTTCACTAAC ACAAAAATTC CAGTTTGCCT TTCCTTCTGA TAAATATGAA
    CAGGATGATC TAAATTTAGA AGGGGTAGGT AATAATGACT TATCACATAT TGCTGGCAAG
    CTGACATATG CTTCTCAGAA ATATAAAAAT CACATTGGCA CTGAGATAGC ACCTGAGAAG
    AGTGTTCCTG ATGATACAAA ATTAGTTAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GTATTCCGGA AAAGATTATT TAAAATATCT GACAATATAC ATGGGAGTGC TTATTCTAAT
    GACAATGAAT TGGACTCTCA CATTGGCTCA GTGAAAATTG TACAAACAGA AATGAACAAA
    GGGAAATCAA GGAACTATAG CAATAGTAAG CAAAAATTTC AGTATTCTGC AAATGTGTTT
    ACAGCAAATA ATGCTTTTTC TGCTTCTGAA ATCGGAGAAG GCATGTTCAA AGCACCATCT
    TTTTCAGTTG CTTTCCAACC TCATGATATT CAAGAGGTAA CAGAAAATGG TTTAGGTTCC
    TTGAAGGCTG TCACAGAAAT TCCGGCAAAA TTTAGAAGTA TTTTCAAAGA ATTTCCATAT
    TTCAACTATA TACAGTCCAA GGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGAAAAACT GTAGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTTGAAT ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCTTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TTACTGGAGA TACAGTAATG GATGATCTAT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTTCAGC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATATTGTAAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TGTTTCTCAG
    ACTTTAAAAA ATACCAGCAC TGCTATTCCA ATGCGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGACCAGC TGTGTGTCTG
    AAAATGGATG AGAGCCATAG ACCAGTGAAA CTTCAGAAAG TGGTCCTTGG ATTTCCCTGC
    AGTAGTAACC AAACTGAGTT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    ATACAAATGT ACTCTGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAGGGTGTG
    CAACAGGCTG CTTCTGTTCT TGTGAAAGAT GCTAAATTTA TTATGACTGT GGAACAGAAA
    CAGAGGTTAC AGAAGTATGC ATATTCCGTA AGAGATTCAA AACTGAGAGA TATCTTAAAA
    GATGGTGCTG CTTATCATCA TGCTGGTATG GAGCTGTCAG ATAGAAAAGT AGTTGAGGGA
    GCTTTTACTG TTGGAGATTT ACCAGTTCTT TTTACTACCA GTACTTTAGC TATGGGAGTA
    AATTTGCCTG CTCACCTAGT AGTTATAAAA TCTACAATGC ATTATGCTGG AGGACTGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CTACTGCAGT TATCATGACT CGATTAAGCA CAAGGGACAA GTACATTCAG
    ATGTTAGCTT GTAGAGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAACATTTA
    AATGCAGAGA TAGTACTGCA TACCATCACG GATGTGAATA TTGCTGTGGA ATGGATACGA
    TCAACTCTGC TTTATATCAG AGCCTTGAAA AATCCATCTC ATTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA AGCAAAATTA CAAGAATTAT GTTTGAAGAA TCTGAATGAT
    TTATCATCCC TGGACTTAAT AAAGATGGAT GAAGGTGTTA ATTTCAAACC AACTGAAGCA
    GGAAGATTGA TGGCTTGGTA TTATATTACA TTTGAGACAG TGAAGAAATT TTATACAATC
    AGTGGAAAAG AAACCTTATC AGATCTGGTT ACATTGATAG CTGGCTGCAA GGAATTTCTA
    GATATACAGT TAAGGATAAA TGAAAAGAAA ACACTGAATA CTTTGAACAA AGATCCAAAT
    CGGATAACTA TCAGATTTCC AATGGAAGGA AGAATTAAAA CAAGAGAAAT GAAAGTAAAT
    TGTCTTATTC AGGCTCAACT AGGATGCATT CCCATACAAG ATTTTGCTTT GACACAAGAT
    ACCGCAAAGA TTTTCAGACA TGGCTCCCGA ATTACAAGAT GGTTGTCAGA TTTTGTAGCT
    GCTCAAGAAA AGAAGTTTGC TGTACTATTG AATAGTTTGA TTTTAGCTAA ATGTTTTAGG
    TGTAAACTTT GGGAAAACTC TCTGCATGTA TCCAAACAAC TGGAAAAAAT TGGTATAACA
    TTGTCAAATG CAATCGTAAA TGCTGGTTTG ACTTCCTTTA AAAAAATAGA AGAGACAGAT
    GCAAGGGAAC TTGAATTGAT TTTAAACAGA CATCCCCCCT TTGGAACCCA GATAAAAGAA
    ACTGTGATGT ATCTACCAAA ATATGAACTT AAAGTGGAAC AGATTACAAG ATATAGTGAT
    ACGACGGCAG AAATATTAGT GACTGTTATA TTAAGAAATT TTGAACAGCT ACAAACTAAA
    AGAACAGCAT CGGATTCTCA CTATGTTACC TTAATCATAG GTGACGCAGA TAATCAAGTA
    GTTTATCTGC ACAAGATTAC GGATTCTGTT TTGCTAAAAG CTGGAAGTTG GGCTAAAAAG
    ATTGCTGTGA AAAGAGCTCT TAAATCTGAA GATCTTAGCA TAAATCTAAT AAGTTCTGAA
    TTTGTTGGGC TTGATATTCA GCAGAAACTT ACAGTCTTTT ACTTAGAACC CAAGAGGTTT
    GGAAATCAAA TCACTATGCA AAGAAAATCT GAAACACAGA TTTCCCATTC TAAACATTCA
    GACATATCTA CAATAGCAGG ACCTAATAAA GGAACGACTG CCAGCAAAAA ACCTGGGAAC
    CGAGAATGCA ATCATCTTTG TAAAAGTAAA CATACATGTG GACATGACTG CTGTAAAATT
    GGAGTTGCAC AGAAGTCAGA AATTAAAGAG TCAACAATTT CTTCATATTT ATCTGATTTA
    AGAAACAGGA ATGCTGTTTC ATCTGTTCCT CCAGTTAAAC GTCTGAAGAT ACAGATGAAT
    AAATCTCAAA GTGTGGACCT TAAAGAGTTT GGTTTTACTC CAAAACCTTC CCTTCCTAGT
    ATATCAAGGT CAGAATATTT AAACATATCT GAATTGCCTA TAATGGAGCA GTGGGATCAG
    CCTGAAATCT ATGGAAAAGT TAGACAAGAA CCATCTGAAT ATCAAGACAA AGAAGTTTTG
    AATGTGAACT TTGAATTGGG AAATGAAGTT TGGGATGATT TTGATGATGA AAACTTAGAA
    GTTACTAGCT TTTCAACTGA TACTGAGAAG ACAAAAATAT CAGGATTTGG AAACACTTTG
    AGTTCAAGTA CCAGGGGAAG TAAGCTACCC CTTCAAGAGT CAAAGAGCAA ATTCCAAAGA
    GAAATGTCAA ACAGTTTTGT TTCATCACAT GAGATGTCGG ATATTTCTTT ATCAAATTCT
    GCTATGCCCA AGTTCAGTGC ATCCTCCATG ACAAAATTAC CTCAACAAGC CGGAAATGCA
    GTTATTGTCC ATTTTCAAGA AAGAAAACCA CAAAATCTGT CACCAGAGAT TGAGAAGCAA
    TGCTTTACTT TCTCTGAAAA AAACCCAAAT TCTTCAAATT ATAAAAAAGT GGATTTTTTT
    ATTAGAAACA GTGAATGTAA AAAGGAAGTT GATTTCAGTA TGTATCATCC TGATGATGAA
    GCTGATGAAA TGAAGTCTTT ATTGGGAATA TTTGATGGTA TTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011539151.1
    CDS183..4490
    Translation

    Target ORF information:

    RefSeq Version XM_011540849.1
    Organism Homo sapiens(human)
    Definition Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011540849.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA ACCATCCAGA CAATGAAAAG TCATTGGATT GGTTTCTCCC TCCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGAG TTAGAGGAAG AATTAGAAAG TCATAAACTG
    TTAGGTCAGG AAAAGAGGCC AAAAATGTTA ACATCAAATT TAAAGATAAC TAATGAAGAT
    ACAAATTATA TTTCACTAAC ACAAAAATTC CAGTTTGCCT TTCCTTCTGA TAAATATGAA
    CAGGATGATC TAAATTTAGA AGGGGTAGGT AATAATGACT TATCACATAT TGCTGGCAAG
    CTGACATATG CTTCTCAGAA ATATAAAAAT CACATTGGCA CTGAGATAGC ACCTGAGAAG
    AGTGTTCCTG ATGATACAAA ATTAGTTAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GTATTCCGGA AAAGATTATT TAAAATATCT GACAATATAC ATGGGAGTGC TTATTCTAAT
    GACAATGAAT TGGACTCTCA CATTGGCTCA GTGAAAATTG TACAAACAGA AATGAACAAA
    GGGAAATCAA GGAACTATAG CAATAGTAAG CAAAAATTTC AGTATTCTGC AAATGTGTTT
    ACAGCAAATA ATGCTTTTTC TGCTTCTGAA ATCGGAGAAG GCATGTTCAA AGCACCATCT
    TTTTCAGTTG CTTTCCAACC TCATGATATT CAAGAGGTAA CAGAAAATGG TTTAGGTTCC
    TTGAAGGCTG TCACAGAAAT TCCGGCAAAA TTTAGAAGTA TTTTCAAAGA ATTTCCATAT
    TTCAACTATA TACAGTCCAA GGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGAAAAACT GTAGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTTGAAT ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCTTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TTACTGGAGA TACAGTAATG GATGATCTAT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTTCAGC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATATTGTAAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TGTTTCTCAG
    ACTTTAAAAA ATACCAGCAC TGCTATTCCA ATGCGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGACCAGC TGTGTGTCTG
    AAAATGGATG AGAGCCATAG ACCAGTGAAA CTTCAGAAAG TGGTCCTTGG ATTTCCCTGC
    AGTAGTAACC AAACTGAGTT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    ATACAAATGT ACTCTGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAGGGTGTG
    CAACAGGCTG CTTCTGTTCT TGTGAAAGAT GCTAAATTTA TTATGACTGT GGAACAGAAA
    CAGAGGTTAC AGAAGTATGC ATATTCCGTA AGAGATTCAA AACTGAGAGA TATCTTAAAA
    GATGGTGCTG CTTATCATCA TGCTGGTATG GAGCTGTCAG ATAGAAAAGT AGTTGAGGGA
    GCTTTTACTG TTGGAGATTT ACCAGTTCTT TTTACTACCA GTACTTTAGC TATGGGAGTA
    AATTTGCCTG CTCACCTAGT AGTTATAAAA TCTACAATGC ATTATGCTGG AGGACTGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CTACTGCAGT TATCATGACT CGATTAAGCA CAAGGGACAA GTACATTCAG
    ATGTTAGCTT GTAGAGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAACATTTA
    AATGCAGAGA TAGTACTGCA TACCATCACG GATGTGAATA TTGCTGTGGA ATGGATACGA
    TCAACTCTGC TTTATATCAG AGCCTTGAAA AATCCATCTC ATTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA AGCAAAATTA CAAGAATTAT GTTTGAAGAA TCTGAATGAT
    TTATCATCCC TGGACTTAAT AAAGATGGAT GAAGGTGTTA ATTTCAAACC AACTGAAGCA
    GGAAGATTGA TGGCTTGGTA TTATATTACA TTTGAGACAG TGAAGAAATT TTATACAATC
    AGTGGAAAAG AAACCTTATC AGATCTGGTT ACATTGATAG CTGGCTGCAA GGAATTTCTA
    GATATACAGT TAAGGATAAA TGAAAAGAAA ACACTGAATA CTTTGAACAA AGATCCAAAT
    CGGATAACTA TCAGATTTCC AATGGAAGGA AGAATTAAAA CAAGAGAAAT GAAAGTAAAT
    TGTCTTATTC AGGCTCAACT AGGATGCATT CCCATACAAG ATTTTGCTTT GACACAAGAT
    ACCGCAAAGA TTTTCAGACA TGGCTCCCGA ATTACAAGAT GGTTGTCAGA TTTTGTAGCT
    GCTCAAGAAA AGAAGTTTGC TGTACTATTG AATAGTTTGA TTTTAGCTAA ATGTTTTAGG
    TGTAAACTTT GGGAAAACTC TCTGCATGTA TCCAAACAAC TGGAAAAAAT TGGTATAACA
    TTGTCAAATG CAATCGTAAA TGCTGGTTTG ACTTCCTTTA AAAAAATAGA AGAGACAGAT
    GCAAGGGAAC TTGAATTGAT TTTAAACAGA CATCCCCCCT TTGGAACCCA GATAAAAGAA
    ACTGTGATGT ATCTACCAAA ATATGAACTT AAAGTGGAAC AGATTACAAG ATATAGTGAT
    ACGACGGCAG AAATATTAGT GACTGTTATA TTAAGAAATT TTGAACAGCT ACAAACTAAA
    AGAACAGCAT CGGATTCTCA CTATGTTACC TTAATCATAG GTGACGCAGA TAATCAAGTA
    GTTTATCTGC ACAAGATTAC GGATTCTGTT TTGCTAAAAG CTGGAAGTTG GGCTAAAAAG
    ATTGCTGTGA AAAGAGCTCT TAAATCTGAA GATCTTAGCA TAAATCTAAT AAGTTCTGAA
    TTTGTTGGGC TTGATATTCA GCAGAAACTT ACAGTCTTTT ACTTAGAACC CAAGAGGTTT
    GGAAATCAAA TCACTATGCA AAGAAAATCT GAAACACAGA TTTCCCATTC TAAACATTCA
    GACATATCTA CAATAGCAGG ACCTAATAAA GGAACGACTG CCAGCAAAAA ACCTGGGAAC
    CGAGAATGCA ATCATCTTTG TAAAAGTAAA CATACATGTG GACATGACTG CTGTAAAATT
    GGAGTTGCAC AGAAGTCAGA AATTAAAGAG TCAACAATTT CTTCATATTT ATCTGATTTA
    AGAAACAGGA ATGCTGTTTC ATCTGTTCCT CCAGTTAAAC GTCTGAAGAT ACAGATGAAT
    AAATCTCAAA GTGTGGACCT TAAAGAGTTT GGTTTTACTC CAAAACCTTC CCTTCCTAGT
    ATATCAAGGT CAGAATATTT AAACATATCT GAATTGCCTA TAATGGAGCA GTGGGATCAG
    CCTGAAATCT ATGGAAAAGT TAGACAAGAA CCATCTGAAT ATCAAGACAA AGAAGTTTTG
    AATGTGAACT TTGAATTGGG AAATGAAGTT TGGGATGATT TTGATGATGA AAACTTAGAA
    GTTACTAGCT TTTCAACTGA TACTGAGAAG ACAAAAATAT CAGGATTTGG AAACACTTTG
    AGTTCAAGTA CCAGGGGAAG TAAGCTACCC CTTCAAGAGT CAAAGAGCAA ATTCCAAAGA
    GAAATGTCAA ACAGTTTTGT TTCATCACAT GAGATGTCGG ATATTTCTTT ATCAAATTCT
    GCTATGCCCA AGTTCAGTGC ATCCTCCATG ACAAAATTAC CTCAACAAGC CGGAAATGCA
    GTTATTGTCC ATTTTCAAGA AAGAAAACCA CAAAATCTGT CACCAGAGAT TGAGAAGCAA
    TGCTTTACTT TCTCTGAAAA AAACCCAAAT TCTTCAAATT ATAAAAAAGT GGATTTTTTT
    ATTAGAAACA GTGAATGTAA AAAGGAAGTT GATTTCAGTA TGTATCATCC TGATGATGAA
    GCTGATGAAA TGAAGTCTTT ATTGGGAATA TTTGATGGTA TTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu77233
    Clone ID Related Accession (Same CDS sequence) XM_011540849.1 , XM_011540850.2 , XM_011540851.1 , XM_011540852.2
    Accession Version XM_011540850.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540850.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA ACCATCCAGA CAATGAAAAG TCATTGGATT GGTTTCTCCC TCCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGAG TTAGAGGAAG AATTAGAAAG TCATAAACTG
    TTAGGTCAGG AAAAGAGGCC AAAAATGTTA ACATCAAATT TAAAGATAAC TAATGAAGAT
    ACAAATTATA TTTCACTAAC ACAAAAATTC CAGTTTGCCT TTCCTTCTGA TAAATATGAA
    CAGGATGATC TAAATTTAGA AGGGGTAGGT AATAATGACT TATCACATAT TGCTGGCAAG
    CTGACATATG CTTCTCAGAA ATATAAAAAT CACATTGGCA CTGAGATAGC ACCTGAGAAG
    AGTGTTCCTG ATGATACAAA ATTAGTTAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GTATTCCGGA AAAGATTATT TAAAATATCT GACAATATAC ATGGGAGTGC TTATTCTAAT
    GACAATGAAT TGGACTCTCA CATTGGCTCA GTGAAAATTG TACAAACAGA AATGAACAAA
    GGGAAATCAA GGAACTATAG CAATAGTAAG CAAAAATTTC AGTATTCTGC AAATGTGTTT
    ACAGCAAATA ATGCTTTTTC TGCTTCTGAA ATCGGAGAAG GCATGTTCAA AGCACCATCT
    TTTTCAGTTG CTTTCCAACC TCATGATATT CAAGAGGTAA CAGAAAATGG TTTAGGTTCC
    TTGAAGGCTG TCACAGAAAT TCCGGCAAAA TTTAGAAGTA TTTTCAAAGA ATTTCCATAT
    TTCAACTATA TACAGTCCAA GGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGAAAAACT GTAGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTTGAAT ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCTTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TTACTGGAGA TACAGTAATG GATGATCTAT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTTCAGC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATATTGTAAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TGTTTCTCAG
    ACTTTAAAAA ATACCAGCAC TGCTATTCCA ATGCGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGACCAGC TGTGTGTCTG
    AAAATGGATG AGAGCCATAG ACCAGTGAAA CTTCAGAAAG TGGTCCTTGG ATTTCCCTGC
    AGTAGTAACC AAACTGAGTT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    ATACAAATGT ACTCTGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAGGGTGTG
    CAACAGGCTG CTTCTGTTCT TGTGAAAGAT GCTAAATTTA TTATGACTGT GGAACAGAAA
    CAGAGGTTAC AGAAGTATGC ATATTCCGTA AGAGATTCAA AACTGAGAGA TATCTTAAAA
    GATGGTGCTG CTTATCATCA TGCTGGTATG GAGCTGTCAG ATAGAAAAGT AGTTGAGGGA
    GCTTTTACTG TTGGAGATTT ACCAGTTCTT TTTACTACCA GTACTTTAGC TATGGGAGTA
    AATTTGCCTG CTCACCTAGT AGTTATAAAA TCTACAATGC ATTATGCTGG AGGACTGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CTACTGCAGT TATCATGACT CGATTAAGCA CAAGGGACAA GTACATTCAG
    ATGTTAGCTT GTAGAGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAACATTTA
    AATGCAGAGA TAGTACTGCA TACCATCACG GATGTGAATA TTGCTGTGGA ATGGATACGA
    TCAACTCTGC TTTATATCAG AGCCTTGAAA AATCCATCTC ATTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA AGCAAAATTA CAAGAATTAT GTTTGAAGAA TCTGAATGAT
    TTATCATCCC TGGACTTAAT AAAGATGGAT GAAGGTGTTA ATTTCAAACC AACTGAAGCA
    GGAAGATTGA TGGCTTGGTA TTATATTACA TTTGAGACAG TGAAGAAATT TTATACAATC
    AGTGGAAAAG AAACCTTATC AGATCTGGTT ACATTGATAG CTGGCTGCAA GGAATTTCTA
    GATATACAGT TAAGGATAAA TGAAAAGAAA ACACTGAATA CTTTGAACAA AGATCCAAAT
    CGGATAACTA TCAGATTTCC AATGGAAGGA AGAATTAAAA CAAGAGAAAT GAAAGTAAAT
    TGTCTTATTC AGGCTCAACT AGGATGCATT CCCATACAAG ATTTTGCTTT GACACAAGAT
    ACCGCAAAGA TTTTCAGACA TGGCTCCCGA ATTACAAGAT GGTTGTCAGA TTTTGTAGCT
    GCTCAAGAAA AGAAGTTTGC TGTACTATTG AATAGTTTGA TTTTAGCTAA ATGTTTTAGG
    TGTAAACTTT GGGAAAACTC TCTGCATGTA TCCAAACAAC TGGAAAAAAT TGGTATAACA
    TTGTCAAATG CAATCGTAAA TGCTGGTTTG ACTTCCTTTA AAAAAATAGA AGAGACAGAT
    GCAAGGGAAC TTGAATTGAT TTTAAACAGA CATCCCCCCT TTGGAACCCA GATAAAAGAA
    ACTGTGATGT ATCTACCAAA ATATGAACTT AAAGTGGAAC AGATTACAAG ATATAGTGAT
    ACGACGGCAG AAATATTAGT GACTGTTATA TTAAGAAATT TTGAACAGCT ACAAACTAAA
    AGAACAGCAT CGGATTCTCA CTATGTTACC TTAATCATAG GTGACGCAGA TAATCAAGTA
    GTTTATCTGC ACAAGATTAC GGATTCTGTT TTGCTAAAAG CTGGAAGTTG GGCTAAAAAG
    ATTGCTGTGA AAAGAGCTCT TAAATCTGAA GATCTTAGCA TAAATCTAAT AAGTTCTGAA
    TTTGTTGGGC TTGATATTCA GCAGAAACTT ACAGTCTTTT ACTTAGAACC CAAGAGGTTT
    GGAAATCAAA TCACTATGCA AAGAAAATCT GAAACACAGA TTTCCCATTC TAAACATTCA
    GACATATCTA CAATAGCAGG ACCTAATAAA GGAACGACTG CCAGCAAAAA ACCTGGGAAC
    CGAGAATGCA ATCATCTTTG TAAAAGTAAA CATACATGTG GACATGACTG CTGTAAAATT
    GGAGTTGCAC AGAAGTCAGA AATTAAAGAG TCAACAATTT CTTCATATTT ATCTGATTTA
    AGAAACAGGA ATGCTGTTTC ATCTGTTCCT CCAGTTAAAC GTCTGAAGAT ACAGATGAAT
    AAATCTCAAA GTGTGGACCT TAAAGAGTTT GGTTTTACTC CAAAACCTTC CCTTCCTAGT
    ATATCAAGGT CAGAATATTT AAACATATCT GAATTGCCTA TAATGGAGCA GTGGGATCAG
    CCTGAAATCT ATGGAAAAGT TAGACAAGAA CCATCTGAAT ATCAAGACAA AGAAGTTTTG
    AATGTGAACT TTGAATTGGG AAATGAAGTT TGGGATGATT TTGATGATGA AAACTTAGAA
    GTTACTAGCT TTTCAACTGA TACTGAGAAG ACAAAAATAT CAGGATTTGG AAACACTTTG
    AGTTCAAGTA CCAGGGGAAG TAAGCTACCC CTTCAAGAGT CAAAGAGCAA ATTCCAAAGA
    GAAATGTCAA ACAGTTTTGT TTCATCACAT GAGATGTCGG ATATTTCTTT ATCAAATTCT
    GCTATGCCCA AGTTCAGTGC ATCCTCCATG ACAAAATTAC CTCAACAAGC CGGAAATGCA
    GTTATTGTCC ATTTTCAAGA AAGAAAACCA CAAAATCTGT CACCAGAGAT TGAGAAGCAA
    TGCTTTACTT TCTCTGAAAA AAACCCAAAT TCTTCAAATT ATAAAAAAGT GGATTTTTTT
    ATTAGAAACA GTGAATGTAA AAAGGAAGTT GATTTCAGTA TGTATCATCC TGATGATGAA
    GCTGATGAAA TGAAGTCTTT ATTGGGAATA TTTGATGGTA TTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011539152.1
    CDS112..4419
    Translation

    Target ORF information:

    RefSeq Version XM_011540850.2
    Organism Homo sapiens(human)
    Definition Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011540850.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA ACCATCCAGA CAATGAAAAG TCATTGGATT GGTTTCTCCC TCCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGAG TTAGAGGAAG AATTAGAAAG TCATAAACTG
    TTAGGTCAGG AAAAGAGGCC AAAAATGTTA ACATCAAATT TAAAGATAAC TAATGAAGAT
    ACAAATTATA TTTCACTAAC ACAAAAATTC CAGTTTGCCT TTCCTTCTGA TAAATATGAA
    CAGGATGATC TAAATTTAGA AGGGGTAGGT AATAATGACT TATCACATAT TGCTGGCAAG
    CTGACATATG CTTCTCAGAA ATATAAAAAT CACATTGGCA CTGAGATAGC ACCTGAGAAG
    AGTGTTCCTG ATGATACAAA ATTAGTTAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GTATTCCGGA AAAGATTATT TAAAATATCT GACAATATAC ATGGGAGTGC TTATTCTAAT
    GACAATGAAT TGGACTCTCA CATTGGCTCA GTGAAAATTG TACAAACAGA AATGAACAAA
    GGGAAATCAA GGAACTATAG CAATAGTAAG CAAAAATTTC AGTATTCTGC AAATGTGTTT
    ACAGCAAATA ATGCTTTTTC TGCTTCTGAA ATCGGAGAAG GCATGTTCAA AGCACCATCT
    TTTTCAGTTG CTTTCCAACC TCATGATATT CAAGAGGTAA CAGAAAATGG TTTAGGTTCC
    TTGAAGGCTG TCACAGAAAT TCCGGCAAAA TTTAGAAGTA TTTTCAAAGA ATTTCCATAT
    TTCAACTATA TACAGTCCAA GGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGAAAAACT GTAGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTTGAAT ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCTTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TTACTGGAGA TACAGTAATG GATGATCTAT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTTCAGC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATATTGTAAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TGTTTCTCAG
    ACTTTAAAAA ATACCAGCAC TGCTATTCCA ATGCGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGACCAGC TGTGTGTCTG
    AAAATGGATG AGAGCCATAG ACCAGTGAAA CTTCAGAAAG TGGTCCTTGG ATTTCCCTGC
    AGTAGTAACC AAACTGAGTT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    ATACAAATGT ACTCTGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAGGGTGTG
    CAACAGGCTG CTTCTGTTCT TGTGAAAGAT GCTAAATTTA TTATGACTGT GGAACAGAAA
    CAGAGGTTAC AGAAGTATGC ATATTCCGTA AGAGATTCAA AACTGAGAGA TATCTTAAAA
    GATGGTGCTG CTTATCATCA TGCTGGTATG GAGCTGTCAG ATAGAAAAGT AGTTGAGGGA
    GCTTTTACTG TTGGAGATTT ACCAGTTCTT TTTACTACCA GTACTTTAGC TATGGGAGTA
    AATTTGCCTG CTCACCTAGT AGTTATAAAA TCTACAATGC ATTATGCTGG AGGACTGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CTACTGCAGT TATCATGACT CGATTAAGCA CAAGGGACAA GTACATTCAG
    ATGTTAGCTT GTAGAGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAACATTTA
    AATGCAGAGA TAGTACTGCA TACCATCACG GATGTGAATA TTGCTGTGGA ATGGATACGA
    TCAACTCTGC TTTATATCAG AGCCTTGAAA AATCCATCTC ATTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA AGCAAAATTA CAAGAATTAT GTTTGAAGAA TCTGAATGAT
    TTATCATCCC TGGACTTAAT AAAGATGGAT GAAGGTGTTA ATTTCAAACC AACTGAAGCA
    GGAAGATTGA TGGCTTGGTA TTATATTACA TTTGAGACAG TGAAGAAATT TTATACAATC
    AGTGGAAAAG AAACCTTATC AGATCTGGTT ACATTGATAG CTGGCTGCAA GGAATTTCTA
    GATATACAGT TAAGGATAAA TGAAAAGAAA ACACTGAATA CTTTGAACAA AGATCCAAAT
    CGGATAACTA TCAGATTTCC AATGGAAGGA AGAATTAAAA CAAGAGAAAT GAAAGTAAAT
    TGTCTTATTC AGGCTCAACT AGGATGCATT CCCATACAAG ATTTTGCTTT GACACAAGAT
    ACCGCAAAGA TTTTCAGACA TGGCTCCCGA ATTACAAGAT GGTTGTCAGA TTTTGTAGCT
    GCTCAAGAAA AGAAGTTTGC TGTACTATTG AATAGTTTGA TTTTAGCTAA ATGTTTTAGG
    TGTAAACTTT GGGAAAACTC TCTGCATGTA TCCAAACAAC TGGAAAAAAT TGGTATAACA
    TTGTCAAATG CAATCGTAAA TGCTGGTTTG ACTTCCTTTA AAAAAATAGA AGAGACAGAT
    GCAAGGGAAC TTGAATTGAT TTTAAACAGA CATCCCCCCT TTGGAACCCA GATAAAAGAA
    ACTGTGATGT ATCTACCAAA ATATGAACTT AAAGTGGAAC AGATTACAAG ATATAGTGAT
    ACGACGGCAG AAATATTAGT GACTGTTATA TTAAGAAATT TTGAACAGCT ACAAACTAAA
    AGAACAGCAT CGGATTCTCA CTATGTTACC TTAATCATAG GTGACGCAGA TAATCAAGTA
    GTTTATCTGC ACAAGATTAC GGATTCTGTT TTGCTAAAAG CTGGAAGTTG GGCTAAAAAG
    ATTGCTGTGA AAAGAGCTCT TAAATCTGAA GATCTTAGCA TAAATCTAAT AAGTTCTGAA
    TTTGTTGGGC TTGATATTCA GCAGAAACTT ACAGTCTTTT ACTTAGAACC CAAGAGGTTT
    GGAAATCAAA TCACTATGCA AAGAAAATCT GAAACACAGA TTTCCCATTC TAAACATTCA
    GACATATCTA CAATAGCAGG ACCTAATAAA GGAACGACTG CCAGCAAAAA ACCTGGGAAC
    CGAGAATGCA ATCATCTTTG TAAAAGTAAA CATACATGTG GACATGACTG CTGTAAAATT
    GGAGTTGCAC AGAAGTCAGA AATTAAAGAG TCAACAATTT CTTCATATTT ATCTGATTTA
    AGAAACAGGA ATGCTGTTTC ATCTGTTCCT CCAGTTAAAC GTCTGAAGAT ACAGATGAAT
    AAATCTCAAA GTGTGGACCT TAAAGAGTTT GGTTTTACTC CAAAACCTTC CCTTCCTAGT
    ATATCAAGGT CAGAATATTT AAACATATCT GAATTGCCTA TAATGGAGCA GTGGGATCAG
    CCTGAAATCT ATGGAAAAGT TAGACAAGAA CCATCTGAAT ATCAAGACAA AGAAGTTTTG
    AATGTGAACT TTGAATTGGG AAATGAAGTT TGGGATGATT TTGATGATGA AAACTTAGAA
    GTTACTAGCT TTTCAACTGA TACTGAGAAG ACAAAAATAT CAGGATTTGG AAACACTTTG
    AGTTCAAGTA CCAGGGGAAG TAAGCTACCC CTTCAAGAGT CAAAGAGCAA ATTCCAAAGA
    GAAATGTCAA ACAGTTTTGT TTCATCACAT GAGATGTCGG ATATTTCTTT ATCAAATTCT
    GCTATGCCCA AGTTCAGTGC ATCCTCCATG ACAAAATTAC CTCAACAAGC CGGAAATGCA
    GTTATTGTCC ATTTTCAAGA AAGAAAACCA CAAAATCTGT CACCAGAGAT TGAGAAGCAA
    TGCTTTACTT TCTCTGAAAA AAACCCAAAT TCTTCAAATT ATAAAAAAGT GGATTTTTTT
    ATTAGAAACA GTGAATGTAA AAAGGAAGTT GATTTCAGTA TGTATCATCC TGATGATGAA
    GCTGATGAAA TGAAGTCTTT ATTGGGAATA TTTGATGGTA TTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu77233
    Clone ID Related Accession (Same CDS sequence) XM_011540849.1 , XM_011540850.2 , XM_011540851.1 , XM_011540852.2
    Accession Version XM_011540851.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA ACCATCCAGA CAATGAAAAG TCATTGGATT GGTTTCTCCC TCCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGAG TTAGAGGAAG AATTAGAAAG TCATAAACTG
    TTAGGTCAGG AAAAGAGGCC AAAAATGTTA ACATCAAATT TAAAGATAAC TAATGAAGAT
    ACAAATTATA TTTCACTAAC ACAAAAATTC CAGTTTGCCT TTCCTTCTGA TAAATATGAA
    CAGGATGATC TAAATTTAGA AGGGGTAGGT AATAATGACT TATCACATAT TGCTGGCAAG
    CTGACATATG CTTCTCAGAA ATATAAAAAT CACATTGGCA CTGAGATAGC ACCTGAGAAG
    AGTGTTCCTG ATGATACAAA ATTAGTTAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GTATTCCGGA AAAGATTATT TAAAATATCT GACAATATAC ATGGGAGTGC TTATTCTAAT
    GACAATGAAT TGGACTCTCA CATTGGCTCA GTGAAAATTG TACAAACAGA AATGAACAAA
    GGGAAATCAA GGAACTATAG CAATAGTAAG CAAAAATTTC AGTATTCTGC AAATGTGTTT
    ACAGCAAATA ATGCTTTTTC TGCTTCTGAA ATCGGAGAAG GCATGTTCAA AGCACCATCT
    TTTTCAGTTG CTTTCCAACC TCATGATATT CAAGAGGTAA CAGAAAATGG TTTAGGTTCC
    TTGAAGGCTG TCACAGAAAT TCCGGCAAAA TTTAGAAGTA TTTTCAAAGA ATTTCCATAT
    TTCAACTATA TACAGTCCAA GGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGAAAAACT GTAGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTTGAAT ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCTTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TTACTGGAGA TACAGTAATG GATGATCTAT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTTCAGC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATATTGTAAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TGTTTCTCAG
    ACTTTAAAAA ATACCAGCAC TGCTATTCCA ATGCGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGACCAGC TGTGTGTCTG
    AAAATGGATG AGAGCCATAG ACCAGTGAAA CTTCAGAAAG TGGTCCTTGG ATTTCCCTGC
    AGTAGTAACC AAACTGAGTT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    ATACAAATGT ACTCTGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAGGGTGTG
    CAACAGGCTG CTTCTGTTCT TGTGAAAGAT GCTAAATTTA TTATGACTGT GGAACAGAAA
    CAGAGGTTAC AGAAGTATGC ATATTCCGTA AGAGATTCAA AACTGAGAGA TATCTTAAAA
    GATGGTGCTG CTTATCATCA TGCTGGTATG GAGCTGTCAG ATAGAAAAGT AGTTGAGGGA
    GCTTTTACTG TTGGAGATTT ACCAGTTCTT TTTACTACCA GTACTTTAGC TATGGGAGTA
    AATTTGCCTG CTCACCTAGT AGTTATAAAA TCTACAATGC ATTATGCTGG AGGACTGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CTACTGCAGT TATCATGACT CGATTAAGCA CAAGGGACAA GTACATTCAG
    ATGTTAGCTT GTAGAGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAACATTTA
    AATGCAGAGA TAGTACTGCA TACCATCACG GATGTGAATA TTGCTGTGGA ATGGATACGA
    TCAACTCTGC TTTATATCAG AGCCTTGAAA AATCCATCTC ATTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA AGCAAAATTA CAAGAATTAT GTTTGAAGAA TCTGAATGAT
    TTATCATCCC TGGACTTAAT AAAGATGGAT GAAGGTGTTA ATTTCAAACC AACTGAAGCA
    GGAAGATTGA TGGCTTGGTA TTATATTACA TTTGAGACAG TGAAGAAATT TTATACAATC
    AGTGGAAAAG AAACCTTATC AGATCTGGTT ACATTGATAG CTGGCTGCAA GGAATTTCTA
    GATATACAGT TAAGGATAAA TGAAAAGAAA ACACTGAATA CTTTGAACAA AGATCCAAAT
    CGGATAACTA TCAGATTTCC AATGGAAGGA AGAATTAAAA CAAGAGAAAT GAAAGTAAAT
    TGTCTTATTC AGGCTCAACT AGGATGCATT CCCATACAAG ATTTTGCTTT GACACAAGAT
    ACCGCAAAGA TTTTCAGACA TGGCTCCCGA ATTACAAGAT GGTTGTCAGA TTTTGTAGCT
    GCTCAAGAAA AGAAGTTTGC TGTACTATTG AATAGTTTGA TTTTAGCTAA ATGTTTTAGG
    TGTAAACTTT GGGAAAACTC TCTGCATGTA TCCAAACAAC TGGAAAAAAT TGGTATAACA
    TTGTCAAATG CAATCGTAAA TGCTGGTTTG ACTTCCTTTA AAAAAATAGA AGAGACAGAT
    GCAAGGGAAC TTGAATTGAT TTTAAACAGA CATCCCCCCT TTGGAACCCA GATAAAAGAA
    ACTGTGATGT ATCTACCAAA ATATGAACTT AAAGTGGAAC AGATTACAAG ATATAGTGAT
    ACGACGGCAG AAATATTAGT GACTGTTATA TTAAGAAATT TTGAACAGCT ACAAACTAAA
    AGAACAGCAT CGGATTCTCA CTATGTTACC TTAATCATAG GTGACGCAGA TAATCAAGTA
    GTTTATCTGC ACAAGATTAC GGATTCTGTT TTGCTAAAAG CTGGAAGTTG GGCTAAAAAG
    ATTGCTGTGA AAAGAGCTCT TAAATCTGAA GATCTTAGCA TAAATCTAAT AAGTTCTGAA
    TTTGTTGGGC TTGATATTCA GCAGAAACTT ACAGTCTTTT ACTTAGAACC CAAGAGGTTT
    GGAAATCAAA TCACTATGCA AAGAAAATCT GAAACACAGA TTTCCCATTC TAAACATTCA
    GACATATCTA CAATAGCAGG ACCTAATAAA GGAACGACTG CCAGCAAAAA ACCTGGGAAC
    CGAGAATGCA ATCATCTTTG TAAAAGTAAA CATACATGTG GACATGACTG CTGTAAAATT
    GGAGTTGCAC AGAAGTCAGA AATTAAAGAG TCAACAATTT CTTCATATTT ATCTGATTTA
    AGAAACAGGA ATGCTGTTTC ATCTGTTCCT CCAGTTAAAC GTCTGAAGAT ACAGATGAAT
    AAATCTCAAA GTGTGGACCT TAAAGAGTTT GGTTTTACTC CAAAACCTTC CCTTCCTAGT
    ATATCAAGGT CAGAATATTT AAACATATCT GAATTGCCTA TAATGGAGCA GTGGGATCAG
    CCTGAAATCT ATGGAAAAGT TAGACAAGAA CCATCTGAAT ATCAAGACAA AGAAGTTTTG
    AATGTGAACT TTGAATTGGG AAATGAAGTT TGGGATGATT TTGATGATGA AAACTTAGAA
    GTTACTAGCT TTTCAACTGA TACTGAGAAG ACAAAAATAT CAGGATTTGG AAACACTTTG
    AGTTCAAGTA CCAGGGGAAG TAAGCTACCC CTTCAAGAGT CAAAGAGCAA ATTCCAAAGA
    GAAATGTCAA ACAGTTTTGT TTCATCACAT GAGATGTCGG ATATTTCTTT ATCAAATTCT
    GCTATGCCCA AGTTCAGTGC ATCCTCCATG ACAAAATTAC CTCAACAAGC CGGAAATGCA
    GTTATTGTCC ATTTTCAAGA AAGAAAACCA CAAAATCTGT CACCAGAGAT TGAGAAGCAA
    TGCTTTACTT TCTCTGAAAA AAACCCAAAT TCTTCAAATT ATAAAAAAGT GGATTTTTTT
    ATTAGAAACA GTGAATGTAA AAAGGAAGTT GATTTCAGTA TGTATCATCC TGATGATGAA
    GCTGATGAAA TGAAGTCTTT ATTGGGAATA TTTGATGGTA TTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011539153.1
    CDS67..4374
    Translation

    Target ORF information:

    RefSeq Version XM_011540851.1
    Organism Homo sapiens(human)
    Definition Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011540851.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA ACCATCCAGA CAATGAAAAG TCATTGGATT GGTTTCTCCC TCCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGAG TTAGAGGAAG AATTAGAAAG TCATAAACTG
    TTAGGTCAGG AAAAGAGGCC AAAAATGTTA ACATCAAATT TAAAGATAAC TAATGAAGAT
    ACAAATTATA TTTCACTAAC ACAAAAATTC CAGTTTGCCT TTCCTTCTGA TAAATATGAA
    CAGGATGATC TAAATTTAGA AGGGGTAGGT AATAATGACT TATCACATAT TGCTGGCAAG
    CTGACATATG CTTCTCAGAA ATATAAAAAT CACATTGGCA CTGAGATAGC ACCTGAGAAG
    AGTGTTCCTG ATGATACAAA ATTAGTTAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GTATTCCGGA AAAGATTATT TAAAATATCT GACAATATAC ATGGGAGTGC TTATTCTAAT
    GACAATGAAT TGGACTCTCA CATTGGCTCA GTGAAAATTG TACAAACAGA AATGAACAAA
    GGGAAATCAA GGAACTATAG CAATAGTAAG CAAAAATTTC AGTATTCTGC AAATGTGTTT
    ACAGCAAATA ATGCTTTTTC TGCTTCTGAA ATCGGAGAAG GCATGTTCAA AGCACCATCT
    TTTTCAGTTG CTTTCCAACC TCATGATATT CAAGAGGTAA CAGAAAATGG TTTAGGTTCC
    TTGAAGGCTG TCACAGAAAT TCCGGCAAAA TTTAGAAGTA TTTTCAAAGA ATTTCCATAT
    TTCAACTATA TACAGTCCAA GGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGAAAAACT GTAGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTTGAAT ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCTTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TTACTGGAGA TACAGTAATG GATGATCTAT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTTCAGC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATATTGTAAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TGTTTCTCAG
    ACTTTAAAAA ATACCAGCAC TGCTATTCCA ATGCGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGACCAGC TGTGTGTCTG
    AAAATGGATG AGAGCCATAG ACCAGTGAAA CTTCAGAAAG TGGTCCTTGG ATTTCCCTGC
    AGTAGTAACC AAACTGAGTT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    ATACAAATGT ACTCTGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAGGGTGTG
    CAACAGGCTG CTTCTGTTCT TGTGAAAGAT GCTAAATTTA TTATGACTGT GGAACAGAAA
    CAGAGGTTAC AGAAGTATGC ATATTCCGTA AGAGATTCAA AACTGAGAGA TATCTTAAAA
    GATGGTGCTG CTTATCATCA TGCTGGTATG GAGCTGTCAG ATAGAAAAGT AGTTGAGGGA
    GCTTTTACTG TTGGAGATTT ACCAGTTCTT TTTACTACCA GTACTTTAGC TATGGGAGTA
    AATTTGCCTG CTCACCTAGT AGTTATAAAA TCTACAATGC ATTATGCTGG AGGACTGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CTACTGCAGT TATCATGACT CGATTAAGCA CAAGGGACAA GTACATTCAG
    ATGTTAGCTT GTAGAGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAACATTTA
    AATGCAGAGA TAGTACTGCA TACCATCACG GATGTGAATA TTGCTGTGGA ATGGATACGA
    TCAACTCTGC TTTATATCAG AGCCTTGAAA AATCCATCTC ATTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA AGCAAAATTA CAAGAATTAT GTTTGAAGAA TCTGAATGAT
    TTATCATCCC TGGACTTAAT AAAGATGGAT GAAGGTGTTA ATTTCAAACC AACTGAAGCA
    GGAAGATTGA TGGCTTGGTA TTATATTACA TTTGAGACAG TGAAGAAATT TTATACAATC
    AGTGGAAAAG AAACCTTATC AGATCTGGTT ACATTGATAG CTGGCTGCAA GGAATTTCTA
    GATATACAGT TAAGGATAAA TGAAAAGAAA ACACTGAATA CTTTGAACAA AGATCCAAAT
    CGGATAACTA TCAGATTTCC AATGGAAGGA AGAATTAAAA CAAGAGAAAT GAAAGTAAAT
    TGTCTTATTC AGGCTCAACT AGGATGCATT CCCATACAAG ATTTTGCTTT GACACAAGAT
    ACCGCAAAGA TTTTCAGACA TGGCTCCCGA ATTACAAGAT GGTTGTCAGA TTTTGTAGCT
    GCTCAAGAAA AGAAGTTTGC TGTACTATTG AATAGTTTGA TTTTAGCTAA ATGTTTTAGG
    TGTAAACTTT GGGAAAACTC TCTGCATGTA TCCAAACAAC TGGAAAAAAT TGGTATAACA
    TTGTCAAATG CAATCGTAAA TGCTGGTTTG ACTTCCTTTA AAAAAATAGA AGAGACAGAT
    GCAAGGGAAC TTGAATTGAT TTTAAACAGA CATCCCCCCT TTGGAACCCA GATAAAAGAA
    ACTGTGATGT ATCTACCAAA ATATGAACTT AAAGTGGAAC AGATTACAAG ATATAGTGAT
    ACGACGGCAG AAATATTAGT GACTGTTATA TTAAGAAATT TTGAACAGCT ACAAACTAAA
    AGAACAGCAT CGGATTCTCA CTATGTTACC TTAATCATAG GTGACGCAGA TAATCAAGTA
    GTTTATCTGC ACAAGATTAC GGATTCTGTT TTGCTAAAAG CTGGAAGTTG GGCTAAAAAG
    ATTGCTGTGA AAAGAGCTCT TAAATCTGAA GATCTTAGCA TAAATCTAAT AAGTTCTGAA
    TTTGTTGGGC TTGATATTCA GCAGAAACTT ACAGTCTTTT ACTTAGAACC CAAGAGGTTT
    GGAAATCAAA TCACTATGCA AAGAAAATCT GAAACACAGA TTTCCCATTC TAAACATTCA
    GACATATCTA CAATAGCAGG ACCTAATAAA GGAACGACTG CCAGCAAAAA ACCTGGGAAC
    CGAGAATGCA ATCATCTTTG TAAAAGTAAA CATACATGTG GACATGACTG CTGTAAAATT
    GGAGTTGCAC AGAAGTCAGA AATTAAAGAG TCAACAATTT CTTCATATTT ATCTGATTTA
    AGAAACAGGA ATGCTGTTTC ATCTGTTCCT CCAGTTAAAC GTCTGAAGAT ACAGATGAAT
    AAATCTCAAA GTGTGGACCT TAAAGAGTTT GGTTTTACTC CAAAACCTTC CCTTCCTAGT
    ATATCAAGGT CAGAATATTT AAACATATCT GAATTGCCTA TAATGGAGCA GTGGGATCAG
    CCTGAAATCT ATGGAAAAGT TAGACAAGAA CCATCTGAAT ATCAAGACAA AGAAGTTTTG
    AATGTGAACT TTGAATTGGG AAATGAAGTT TGGGATGATT TTGATGATGA AAACTTAGAA
    GTTACTAGCT TTTCAACTGA TACTGAGAAG ACAAAAATAT CAGGATTTGG AAACACTTTG
    AGTTCAAGTA CCAGGGGAAG TAAGCTACCC CTTCAAGAGT CAAAGAGCAA ATTCCAAAGA
    GAAATGTCAA ACAGTTTTGT TTCATCACAT GAGATGTCGG ATATTTCTTT ATCAAATTCT
    GCTATGCCCA AGTTCAGTGC ATCCTCCATG ACAAAATTAC CTCAACAAGC CGGAAATGCA
    GTTATTGTCC ATTTTCAAGA AAGAAAACCA CAAAATCTGT CACCAGAGAT TGAGAAGCAA
    TGCTTTACTT TCTCTGAAAA AAACCCAAAT TCTTCAAATT ATAAAAAAGT GGATTTTTTT
    ATTAGAAACA GTGAATGTAA AAAGGAAGTT GATTTCAGTA TGTATCATCC TGATGATGAA
    GCTGATGAAA TGAAGTCTTT ATTGGGAATA TTTGATGGTA TTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu77233
    Clone ID Related Accession (Same CDS sequence) XM_011540849.1 , XM_011540850.2 , XM_011540851.1 , XM_011540852.2
    Accession Version XM_011540852.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540852.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA ACCATCCAGA CAATGAAAAG TCATTGGATT GGTTTCTCCC TCCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGAG TTAGAGGAAG AATTAGAAAG TCATAAACTG
    TTAGGTCAGG AAAAGAGGCC AAAAATGTTA ACATCAAATT TAAAGATAAC TAATGAAGAT
    ACAAATTATA TTTCACTAAC ACAAAAATTC CAGTTTGCCT TTCCTTCTGA TAAATATGAA
    CAGGATGATC TAAATTTAGA AGGGGTAGGT AATAATGACT TATCACATAT TGCTGGCAAG
    CTGACATATG CTTCTCAGAA ATATAAAAAT CACATTGGCA CTGAGATAGC ACCTGAGAAG
    AGTGTTCCTG ATGATACAAA ATTAGTTAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GTATTCCGGA AAAGATTATT TAAAATATCT GACAATATAC ATGGGAGTGC TTATTCTAAT
    GACAATGAAT TGGACTCTCA CATTGGCTCA GTGAAAATTG TACAAACAGA AATGAACAAA
    GGGAAATCAA GGAACTATAG CAATAGTAAG CAAAAATTTC AGTATTCTGC AAATGTGTTT
    ACAGCAAATA ATGCTTTTTC TGCTTCTGAA ATCGGAGAAG GCATGTTCAA AGCACCATCT
    TTTTCAGTTG CTTTCCAACC TCATGATATT CAAGAGGTAA CAGAAAATGG TTTAGGTTCC
    TTGAAGGCTG TCACAGAAAT TCCGGCAAAA TTTAGAAGTA TTTTCAAAGA ATTTCCATAT
    TTCAACTATA TACAGTCCAA GGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGAAAAACT GTAGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTTGAAT ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCTTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TTACTGGAGA TACAGTAATG GATGATCTAT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTTCAGC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATATTGTAAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TGTTTCTCAG
    ACTTTAAAAA ATACCAGCAC TGCTATTCCA ATGCGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGACCAGC TGTGTGTCTG
    AAAATGGATG AGAGCCATAG ACCAGTGAAA CTTCAGAAAG TGGTCCTTGG ATTTCCCTGC
    AGTAGTAACC AAACTGAGTT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    ATACAAATGT ACTCTGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAGGGTGTG
    CAACAGGCTG CTTCTGTTCT TGTGAAAGAT GCTAAATTTA TTATGACTGT GGAACAGAAA
    CAGAGGTTAC AGAAGTATGC ATATTCCGTA AGAGATTCAA AACTGAGAGA TATCTTAAAA
    GATGGTGCTG CTTATCATCA TGCTGGTATG GAGCTGTCAG ATAGAAAAGT AGTTGAGGGA
    GCTTTTACTG TTGGAGATTT ACCAGTTCTT TTTACTACCA GTACTTTAGC TATGGGAGTA
    AATTTGCCTG CTCACCTAGT AGTTATAAAA TCTACAATGC ATTATGCTGG AGGACTGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CTACTGCAGT TATCATGACT CGATTAAGCA CAAGGGACAA GTACATTCAG
    ATGTTAGCTT GTAGAGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAACATTTA
    AATGCAGAGA TAGTACTGCA TACCATCACG GATGTGAATA TTGCTGTGGA ATGGATACGA
    TCAACTCTGC TTTATATCAG AGCCTTGAAA AATCCATCTC ATTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA AGCAAAATTA CAAGAATTAT GTTTGAAGAA TCTGAATGAT
    TTATCATCCC TGGACTTAAT AAAGATGGAT GAAGGTGTTA ATTTCAAACC AACTGAAGCA
    GGAAGATTGA TGGCTTGGTA TTATATTACA TTTGAGACAG TGAAGAAATT TTATACAATC
    AGTGGAAAAG AAACCTTATC AGATCTGGTT ACATTGATAG CTGGCTGCAA GGAATTTCTA
    GATATACAGT TAAGGATAAA TGAAAAGAAA ACACTGAATA CTTTGAACAA AGATCCAAAT
    CGGATAACTA TCAGATTTCC AATGGAAGGA AGAATTAAAA CAAGAGAAAT GAAAGTAAAT
    TGTCTTATTC AGGCTCAACT AGGATGCATT CCCATACAAG ATTTTGCTTT GACACAAGAT
    ACCGCAAAGA TTTTCAGACA TGGCTCCCGA ATTACAAGAT GGTTGTCAGA TTTTGTAGCT
    GCTCAAGAAA AGAAGTTTGC TGTACTATTG AATAGTTTGA TTTTAGCTAA ATGTTTTAGG
    TGTAAACTTT GGGAAAACTC TCTGCATGTA TCCAAACAAC TGGAAAAAAT TGGTATAACA
    TTGTCAAATG CAATCGTAAA TGCTGGTTTG ACTTCCTTTA AAAAAATAGA AGAGACAGAT
    GCAAGGGAAC TTGAATTGAT TTTAAACAGA CATCCCCCCT TTGGAACCCA GATAAAAGAA
    ACTGTGATGT ATCTACCAAA ATATGAACTT AAAGTGGAAC AGATTACAAG ATATAGTGAT
    ACGACGGCAG AAATATTAGT GACTGTTATA TTAAGAAATT TTGAACAGCT ACAAACTAAA
    AGAACAGCAT CGGATTCTCA CTATGTTACC TTAATCATAG GTGACGCAGA TAATCAAGTA
    GTTTATCTGC ACAAGATTAC GGATTCTGTT TTGCTAAAAG CTGGAAGTTG GGCTAAAAAG
    ATTGCTGTGA AAAGAGCTCT TAAATCTGAA GATCTTAGCA TAAATCTAAT AAGTTCTGAA
    TTTGTTGGGC TTGATATTCA GCAGAAACTT ACAGTCTTTT ACTTAGAACC CAAGAGGTTT
    GGAAATCAAA TCACTATGCA AAGAAAATCT GAAACACAGA TTTCCCATTC TAAACATTCA
    GACATATCTA CAATAGCAGG ACCTAATAAA GGAACGACTG CCAGCAAAAA ACCTGGGAAC
    CGAGAATGCA ATCATCTTTG TAAAAGTAAA CATACATGTG GACATGACTG CTGTAAAATT
    GGAGTTGCAC AGAAGTCAGA AATTAAAGAG TCAACAATTT CTTCATATTT ATCTGATTTA
    AGAAACAGGA ATGCTGTTTC ATCTGTTCCT CCAGTTAAAC GTCTGAAGAT ACAGATGAAT
    AAATCTCAAA GTGTGGACCT TAAAGAGTTT GGTTTTACTC CAAAACCTTC CCTTCCTAGT
    ATATCAAGGT CAGAATATTT AAACATATCT GAATTGCCTA TAATGGAGCA GTGGGATCAG
    CCTGAAATCT ATGGAAAAGT TAGACAAGAA CCATCTGAAT ATCAAGACAA AGAAGTTTTG
    AATGTGAACT TTGAATTGGG AAATGAAGTT TGGGATGATT TTGATGATGA AAACTTAGAA
    GTTACTAGCT TTTCAACTGA TACTGAGAAG ACAAAAATAT CAGGATTTGG AAACACTTTG
    AGTTCAAGTA CCAGGGGAAG TAAGCTACCC CTTCAAGAGT CAAAGAGCAA ATTCCAAAGA
    GAAATGTCAA ACAGTTTTGT TTCATCACAT GAGATGTCGG ATATTTCTTT ATCAAATTCT
    GCTATGCCCA AGTTCAGTGC ATCCTCCATG ACAAAATTAC CTCAACAAGC CGGAAATGCA
    GTTATTGTCC ATTTTCAAGA AAGAAAACCA CAAAATCTGT CACCAGAGAT TGAGAAGCAA
    TGCTTTACTT TCTCTGAAAA AAACCCAAAT TCTTCAAATT ATAAAAAAGT GGATTTTTTT
    ATTAGAAACA GTGAATGTAA AAAGGAAGTT GATTTCAGTA TGTATCATCC TGATGATGAA
    GCTGATGAAA TGAAGTCTTT ATTGGGAATA TTTGATGGTA TTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011539154.1
    CDS49..4356
    Translation

    Target ORF information:

    RefSeq Version XM_011540852.2
    Organism Homo sapiens(human)
    Definition Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011540852.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA ACCATCCAGA CAATGAAAAG TCATTGGATT GGTTTCTCCC TCCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGAG TTAGAGGAAG AATTAGAAAG TCATAAACTG
    TTAGGTCAGG AAAAGAGGCC AAAAATGTTA ACATCAAATT TAAAGATAAC TAATGAAGAT
    ACAAATTATA TTTCACTAAC ACAAAAATTC CAGTTTGCCT TTCCTTCTGA TAAATATGAA
    CAGGATGATC TAAATTTAGA AGGGGTAGGT AATAATGACT TATCACATAT TGCTGGCAAG
    CTGACATATG CTTCTCAGAA ATATAAAAAT CACATTGGCA CTGAGATAGC ACCTGAGAAG
    AGTGTTCCTG ATGATACAAA ATTAGTTAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GTATTCCGGA AAAGATTATT TAAAATATCT GACAATATAC ATGGGAGTGC TTATTCTAAT
    GACAATGAAT TGGACTCTCA CATTGGCTCA GTGAAAATTG TACAAACAGA AATGAACAAA
    GGGAAATCAA GGAACTATAG CAATAGTAAG CAAAAATTTC AGTATTCTGC AAATGTGTTT
    ACAGCAAATA ATGCTTTTTC TGCTTCTGAA ATCGGAGAAG GCATGTTCAA AGCACCATCT
    TTTTCAGTTG CTTTCCAACC TCATGATATT CAAGAGGTAA CAGAAAATGG TTTAGGTTCC
    TTGAAGGCTG TCACAGAAAT TCCGGCAAAA TTTAGAAGTA TTTTCAAAGA ATTTCCATAT
    TTCAACTATA TACAGTCCAA GGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGAAAAACT GTAGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTTGAAT ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCTTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TTACTGGAGA TACAGTAATG GATGATCTAT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTTCAGC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATATTGTAAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TGTTTCTCAG
    ACTTTAAAAA ATACCAGCAC TGCTATTCCA ATGCGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGACCAGC TGTGTGTCTG
    AAAATGGATG AGAGCCATAG ACCAGTGAAA CTTCAGAAAG TGGTCCTTGG ATTTCCCTGC
    AGTAGTAACC AAACTGAGTT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    ATACAAATGT ACTCTGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAGGGTGTG
    CAACAGGCTG CTTCTGTTCT TGTGAAAGAT GCTAAATTTA TTATGACTGT GGAACAGAAA
    CAGAGGTTAC AGAAGTATGC ATATTCCGTA AGAGATTCAA AACTGAGAGA TATCTTAAAA
    GATGGTGCTG CTTATCATCA TGCTGGTATG GAGCTGTCAG ATAGAAAAGT AGTTGAGGGA
    GCTTTTACTG TTGGAGATTT ACCAGTTCTT TTTACTACCA GTACTTTAGC TATGGGAGTA
    AATTTGCCTG CTCACCTAGT AGTTATAAAA TCTACAATGC ATTATGCTGG AGGACTGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CTACTGCAGT TATCATGACT CGATTAAGCA CAAGGGACAA GTACATTCAG
    ATGTTAGCTT GTAGAGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAACATTTA
    AATGCAGAGA TAGTACTGCA TACCATCACG GATGTGAATA TTGCTGTGGA ATGGATACGA
    TCAACTCTGC TTTATATCAG AGCCTTGAAA AATCCATCTC ATTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA AGCAAAATTA CAAGAATTAT GTTTGAAGAA TCTGAATGAT
    TTATCATCCC TGGACTTAAT AAAGATGGAT GAAGGTGTTA ATTTCAAACC AACTGAAGCA
    GGAAGATTGA TGGCTTGGTA TTATATTACA TTTGAGACAG TGAAGAAATT TTATACAATC
    AGTGGAAAAG AAACCTTATC AGATCTGGTT ACATTGATAG CTGGCTGCAA GGAATTTCTA
    GATATACAGT TAAGGATAAA TGAAAAGAAA ACACTGAATA CTTTGAACAA AGATCCAAAT
    CGGATAACTA TCAGATTTCC AATGGAAGGA AGAATTAAAA CAAGAGAAAT GAAAGTAAAT
    TGTCTTATTC AGGCTCAACT AGGATGCATT CCCATACAAG ATTTTGCTTT GACACAAGAT
    ACCGCAAAGA TTTTCAGACA TGGCTCCCGA ATTACAAGAT GGTTGTCAGA TTTTGTAGCT
    GCTCAAGAAA AGAAGTTTGC TGTACTATTG AATAGTTTGA TTTTAGCTAA ATGTTTTAGG
    TGTAAACTTT GGGAAAACTC TCTGCATGTA TCCAAACAAC TGGAAAAAAT TGGTATAACA
    TTGTCAAATG CAATCGTAAA TGCTGGTTTG ACTTCCTTTA AAAAAATAGA AGAGACAGAT
    GCAAGGGAAC TTGAATTGAT TTTAAACAGA CATCCCCCCT TTGGAACCCA GATAAAAGAA
    ACTGTGATGT ATCTACCAAA ATATGAACTT AAAGTGGAAC AGATTACAAG ATATAGTGAT
    ACGACGGCAG AAATATTAGT GACTGTTATA TTAAGAAATT TTGAACAGCT ACAAACTAAA
    AGAACAGCAT CGGATTCTCA CTATGTTACC TTAATCATAG GTGACGCAGA TAATCAAGTA
    GTTTATCTGC ACAAGATTAC GGATTCTGTT TTGCTAAAAG CTGGAAGTTG GGCTAAAAAG
    ATTGCTGTGA AAAGAGCTCT TAAATCTGAA GATCTTAGCA TAAATCTAAT AAGTTCTGAA
    TTTGTTGGGC TTGATATTCA GCAGAAACTT ACAGTCTTTT ACTTAGAACC CAAGAGGTTT
    GGAAATCAAA TCACTATGCA AAGAAAATCT GAAACACAGA TTTCCCATTC TAAACATTCA
    GACATATCTA CAATAGCAGG ACCTAATAAA GGAACGACTG CCAGCAAAAA ACCTGGGAAC
    CGAGAATGCA ATCATCTTTG TAAAAGTAAA CATACATGTG GACATGACTG CTGTAAAATT
    GGAGTTGCAC AGAAGTCAGA AATTAAAGAG TCAACAATTT CTTCATATTT ATCTGATTTA
    AGAAACAGGA ATGCTGTTTC ATCTGTTCCT CCAGTTAAAC GTCTGAAGAT ACAGATGAAT
    AAATCTCAAA GTGTGGACCT TAAAGAGTTT GGTTTTACTC CAAAACCTTC CCTTCCTAGT
    ATATCAAGGT CAGAATATTT AAACATATCT GAATTGCCTA TAATGGAGCA GTGGGATCAG
    CCTGAAATCT ATGGAAAAGT TAGACAAGAA CCATCTGAAT ATCAAGACAA AGAAGTTTTG
    AATGTGAACT TTGAATTGGG AAATGAAGTT TGGGATGATT TTGATGATGA AAACTTAGAA
    GTTACTAGCT TTTCAACTGA TACTGAGAAG ACAAAAATAT CAGGATTTGG AAACACTTTG
    AGTTCAAGTA CCAGGGGAAG TAAGCTACCC CTTCAAGAGT CAAAGAGCAA ATTCCAAAGA
    GAAATGTCAA ACAGTTTTGT TTCATCACAT GAGATGTCGG ATATTTCTTT ATCAAATTCT
    GCTATGCCCA AGTTCAGTGC ATCCTCCATG ACAAAATTAC CTCAACAAGC CGGAAATGCA
    GTTATTGTCC ATTTTCAAGA AAGAAAACCA CAAAATCTGT CACCAGAGAT TGAGAAGCAA
    TGCTTTACTT TCTCTGAAAA AAACCCAAAT TCTTCAAATT ATAAAAAAGT GGATTTTTTT
    ATTAGAAACA GTGAATGTAA AAAGGAAGTT GATTTCAGTA TGTATCATCC TGATGATGAA
    GCTGATGAAA TGAAGTCTTT ATTGGGAATA TTTGATGGTA TTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu77241
    Clone ID Related Accession (Same CDS sequence) XM_017000496.1 , XM_017000495.1
    Accession Version XM_017000496.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2451bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product probable ATP-dependent DNA helicase HFM1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA ACCATCCAGA CAATGAAAAG TCATTGGATT GGTTTCTCCC TCCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGAG TTAGAGGAAG AATTAGAAAG TCATAAACTG
    TTAGGTCAGG AAAAGAGGCC AAAAATGTTA ACATCAAATT TAAAGATAAC TAATGAAGAT
    ACAAATTATA TTTCACTAAC ACAAAAATTC CAGTTTGCCT TTCCTTCTGA TAAATATGAA
    CAGGATGATC TAAATTTAGA AGGGGTAGGT AATAATGACT TATCACATAT TGCTGGCAAG
    CTGACATATG CTTCTCAGAA ATATAAAAAT CACATTGGCA CTGAGATAGC ACCTGAGAAG
    AGTGTTCCTG ATGATACAAA ATTAGTTAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GTATTCCGGA AAAGATTATT TAAAATATCT GACAATATAC ATGGGAGTGC TTATTCTAAT
    GACAATGAAT TGGACTCTCA CATTGGCTCA GTGAAAATTG TACAAACAGA AATGAACAAA
    GGGAAATCAA GGAACTATAG CAATAGTAAG CAAAAATTTC AGTATTCTGC AAATGTGTTT
    ACAGCAAATA ATGCTTTTTC TGCTTCTGAA ATCGGAGAAG GCATGTTCAA AGCACCATCT
    TTTTCAGTTG CTTTCCAACC TCATGATATT CAAGAGGTAA CAGAAAATGG TTTAGGTTCC
    TTGAAGGCTG TCACAGAAAT TCCGGCAAAA TTTAGAAGTA TTTTCAAAGA ATTTCCATAT
    TTCAACTATA TACAGTCCAA GGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGAAAAACT GTAGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTTGAAT ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCTTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TTACTGGAGA TACAGTAATG GATGATCTAT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTTCAGC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATATTGTAAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TGTTTCTCAG
    ACTTTAAAAA ATACCAGCAC TGCTATTCCA ATGCGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGACCAGC TGTGTGTCTG
    AAAATGGATG AGAGCCATAG ACCAGTGAAA CTTCAGAAAG TGGTCCTTGG ATTTCCCTGC
    AGTAGTAACC AAACTGAGTT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    ATACAAATGT ACTCTGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAGGGTGTG
    CAACAGGCTG CTTCTGTTCT TGTGAAAGAT GCTAAATTTA TTATGACTGT GGAACAGAAA
    CAGAGGTTAC AGAAGTATGC ATATTCCGTA AGAGATTCAA AACTGAGAGA TATCTTAAAA
    GATGGTGCTG CTTATCATCA TGCTGGTATG GAGCTGTCAG ATAGAAAAGT AGTTGAGGGA
    GCTTTTACTG TTGGAGATTT ACCAGTTCTT TTTACTACCA GTACTTTAGC TATGGGAGTA
    AATTTGCCTG CTCACCTAGT AGTTATAAAA TCTACAATGC ATTATGCTGG AGGACTGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CTACTGCAGT TATCATGACT CGATTAAGCA CAAGGGACAA GTACATTCAG
    ATGTTAGCTT GTAGAGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAACATTTA
    AATGCAGAGA TAGTACTGCA TACCATCACG GATGTGAATA TTGCTGTGGA ATGGATACGA
    TCAACTCTGC TTTATATCAG AGCCTTGAAA AATCCATCTC ATTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA AGCAAAATTA CAAGAATTAT GTTTGAAGAA TCTGAATGAT
    TTATCATCCC TGGACTTAAT AAAGATGGAT GAAGGTGTTA ATTTCAAACC AACTGAAGCA
    GGAAGATTGA TGGCTTGGTA TTATATTACA TTTGAGACAG TGAAGAAATT TTATACAATC
    AGTGGAAAAG AAACCTTATC AGATCTGATT TCCAATGGAA GGAAGAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016855985.1
    CDS44..2494
    Translation

    Target ORF information:

    RefSeq Version XM_017000496.1
    Organism Homo sapiens(human)
    Definition Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017000496.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA ACCATCCAGA CAATGAAAAG TCATTGGATT GGTTTCTCCC TCCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGAG TTAGAGGAAG AATTAGAAAG TCATAAACTG
    TTAGGTCAGG AAAAGAGGCC AAAAATGTTA ACATCAAATT TAAAGATAAC TAATGAAGAT
    ACAAATTATA TTTCACTAAC ACAAAAATTC CAGTTTGCCT TTCCTTCTGA TAAATATGAA
    CAGGATGATC TAAATTTAGA AGGGGTAGGT AATAATGACT TATCACATAT TGCTGGCAAG
    CTGACATATG CTTCTCAGAA ATATAAAAAT CACATTGGCA CTGAGATAGC ACCTGAGAAG
    AGTGTTCCTG ATGATACAAA ATTAGTTAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GTATTCCGGA AAAGATTATT TAAAATATCT GACAATATAC ATGGGAGTGC TTATTCTAAT
    GACAATGAAT TGGACTCTCA CATTGGCTCA GTGAAAATTG TACAAACAGA AATGAACAAA
    GGGAAATCAA GGAACTATAG CAATAGTAAG CAAAAATTTC AGTATTCTGC AAATGTGTTT
    ACAGCAAATA ATGCTTTTTC TGCTTCTGAA ATCGGAGAAG GCATGTTCAA AGCACCATCT
    TTTTCAGTTG CTTTCCAACC TCATGATATT CAAGAGGTAA CAGAAAATGG TTTAGGTTCC
    TTGAAGGCTG TCACAGAAAT TCCGGCAAAA TTTAGAAGTA TTTTCAAAGA ATTTCCATAT
    TTCAACTATA TACAGTCCAA GGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGAAAAACT GTAGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTTGAAT ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCTTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TTACTGGAGA TACAGTAATG GATGATCTAT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTTCAGC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATATTGTAAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TGTTTCTCAG
    ACTTTAAAAA ATACCAGCAC TGCTATTCCA ATGCGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGACCAGC TGTGTGTCTG
    AAAATGGATG AGAGCCATAG ACCAGTGAAA CTTCAGAAAG TGGTCCTTGG ATTTCCCTGC
    AGTAGTAACC AAACTGAGTT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    ATACAAATGT ACTCTGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAGGGTGTG
    CAACAGGCTG CTTCTGTTCT TGTGAAAGAT GCTAAATTTA TTATGACTGT GGAACAGAAA
    CAGAGGTTAC AGAAGTATGC ATATTCCGTA AGAGATTCAA AACTGAGAGA TATCTTAAAA
    GATGGTGCTG CTTATCATCA TGCTGGTATG GAGCTGTCAG ATAGAAAAGT AGTTGAGGGA
    GCTTTTACTG TTGGAGATTT ACCAGTTCTT TTTACTACCA GTACTTTAGC TATGGGAGTA
    AATTTGCCTG CTCACCTAGT AGTTATAAAA TCTACAATGC ATTATGCTGG AGGACTGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CTACTGCAGT TATCATGACT CGATTAAGCA CAAGGGACAA GTACATTCAG
    ATGTTAGCTT GTAGAGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAACATTTA
    AATGCAGAGA TAGTACTGCA TACCATCACG GATGTGAATA TTGCTGTGGA ATGGATACGA
    TCAACTCTGC TTTATATCAG AGCCTTGAAA AATCCATCTC ATTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA AGCAAAATTA CAAGAATTAT GTTTGAAGAA TCTGAATGAT
    TTATCATCCC TGGACTTAAT AAAGATGGAT GAAGGTGTTA ATTTCAAACC AACTGAAGCA
    GGAAGATTGA TGGCTTGGTA TTATATTACA TTTGAGACAG TGAAGAAATT TTATACAATC
    AGTGGAAAAG AAACCTTATC AGATCTGATT TCCAATGGAA GGAAGAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu77241
    Clone ID Related Accession (Same CDS sequence) XM_017000496.1 , XM_017000495.1
    Accession Version XM_017000495.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2451bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product probable ATP-dependent DNA helicase HFM1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA ACCATCCAGA CAATGAAAAG TCATTGGATT GGTTTCTCCC TCCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGAG TTAGAGGAAG AATTAGAAAG TCATAAACTG
    TTAGGTCAGG AAAAGAGGCC AAAAATGTTA ACATCAAATT TAAAGATAAC TAATGAAGAT
    ACAAATTATA TTTCACTAAC ACAAAAATTC CAGTTTGCCT TTCCTTCTGA TAAATATGAA
    CAGGATGATC TAAATTTAGA AGGGGTAGGT AATAATGACT TATCACATAT TGCTGGCAAG
    CTGACATATG CTTCTCAGAA ATATAAAAAT CACATTGGCA CTGAGATAGC ACCTGAGAAG
    AGTGTTCCTG ATGATACAAA ATTAGTTAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GTATTCCGGA AAAGATTATT TAAAATATCT GACAATATAC ATGGGAGTGC TTATTCTAAT
    GACAATGAAT TGGACTCTCA CATTGGCTCA GTGAAAATTG TACAAACAGA AATGAACAAA
    GGGAAATCAA GGAACTATAG CAATAGTAAG CAAAAATTTC AGTATTCTGC AAATGTGTTT
    ACAGCAAATA ATGCTTTTTC TGCTTCTGAA ATCGGAGAAG GCATGTTCAA AGCACCATCT
    TTTTCAGTTG CTTTCCAACC TCATGATATT CAAGAGGTAA CAGAAAATGG TTTAGGTTCC
    TTGAAGGCTG TCACAGAAAT TCCGGCAAAA TTTAGAAGTA TTTTCAAAGA ATTTCCATAT
    TTCAACTATA TACAGTCCAA GGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGAAAAACT GTAGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTTGAAT ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCTTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TTACTGGAGA TACAGTAATG GATGATCTAT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTTCAGC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATATTGTAAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TGTTTCTCAG
    ACTTTAAAAA ATACCAGCAC TGCTATTCCA ATGCGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGACCAGC TGTGTGTCTG
    AAAATGGATG AGAGCCATAG ACCAGTGAAA CTTCAGAAAG TGGTCCTTGG ATTTCCCTGC
    AGTAGTAACC AAACTGAGTT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    ATACAAATGT ACTCTGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAGGGTGTG
    CAACAGGCTG CTTCTGTTCT TGTGAAAGAT GCTAAATTTA TTATGACTGT GGAACAGAAA
    CAGAGGTTAC AGAAGTATGC ATATTCCGTA AGAGATTCAA AACTGAGAGA TATCTTAAAA
    GATGGTGCTG CTTATCATCA TGCTGGTATG GAGCTGTCAG ATAGAAAAGT AGTTGAGGGA
    GCTTTTACTG TTGGAGATTT ACCAGTTCTT TTTACTACCA GTACTTTAGC TATGGGAGTA
    AATTTGCCTG CTCACCTAGT AGTTATAAAA TCTACAATGC ATTATGCTGG AGGACTGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CTACTGCAGT TATCATGACT CGATTAAGCA CAAGGGACAA GTACATTCAG
    ATGTTAGCTT GTAGAGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAACATTTA
    AATGCAGAGA TAGTACTGCA TACCATCACG GATGTGAATA TTGCTGTGGA ATGGATACGA
    TCAACTCTGC TTTATATCAG AGCCTTGAAA AATCCATCTC ATTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA AGCAAAATTA CAAGAATTAT GTTTGAAGAA TCTGAATGAT
    TTATCATCCC TGGACTTAAT AAAGATGGAT GAAGGTGTTA ATTTCAAACC AACTGAAGCA
    GGAAGATTGA TGGCTTGGTA TTATATTACA TTTGAGACAG TGAAGAAATT TTATACAATC
    AGTGGAAAAG AAACCTTATC AGATCTGATT TCCAATGGAA GGAAGAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016855984.1
    CDS44..2494
    Translation

    Target ORF information:

    RefSeq Version XM_017000495.1
    Organism Homo sapiens(human)
    Definition Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017000495.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA ACCATCCAGA CAATGAAAAG TCATTGGATT GGTTTCTCCC TCCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGAG TTAGAGGAAG AATTAGAAAG TCATAAACTG
    TTAGGTCAGG AAAAGAGGCC AAAAATGTTA ACATCAAATT TAAAGATAAC TAATGAAGAT
    ACAAATTATA TTTCACTAAC ACAAAAATTC CAGTTTGCCT TTCCTTCTGA TAAATATGAA
    CAGGATGATC TAAATTTAGA AGGGGTAGGT AATAATGACT TATCACATAT TGCTGGCAAG
    CTGACATATG CTTCTCAGAA ATATAAAAAT CACATTGGCA CTGAGATAGC ACCTGAGAAG
    AGTGTTCCTG ATGATACAAA ATTAGTTAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GTATTCCGGA AAAGATTATT TAAAATATCT GACAATATAC ATGGGAGTGC TTATTCTAAT
    GACAATGAAT TGGACTCTCA CATTGGCTCA GTGAAAATTG TACAAACAGA AATGAACAAA
    GGGAAATCAA GGAACTATAG CAATAGTAAG CAAAAATTTC AGTATTCTGC AAATGTGTTT
    ACAGCAAATA ATGCTTTTTC TGCTTCTGAA ATCGGAGAAG GCATGTTCAA AGCACCATCT
    TTTTCAGTTG CTTTCCAACC TCATGATATT CAAGAGGTAA CAGAAAATGG TTTAGGTTCC
    TTGAAGGCTG TCACAGAAAT TCCGGCAAAA TTTAGAAGTA TTTTCAAAGA ATTTCCATAT
    TTCAACTATA TACAGTCCAA GGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGAAAAACT GTAGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTTGAAT ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCTTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TTACTGGAGA TACAGTAATG GATGATCTAT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTTCAGC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATATTGTAAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TGTTTCTCAG
    ACTTTAAAAA ATACCAGCAC TGCTATTCCA ATGCGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGACCAGC TGTGTGTCTG
    AAAATGGATG AGAGCCATAG ACCAGTGAAA CTTCAGAAAG TGGTCCTTGG ATTTCCCTGC
    AGTAGTAACC AAACTGAGTT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    ATACAAATGT ACTCTGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAGGGTGTG
    CAACAGGCTG CTTCTGTTCT TGTGAAAGAT GCTAAATTTA TTATGACTGT GGAACAGAAA
    CAGAGGTTAC AGAAGTATGC ATATTCCGTA AGAGATTCAA AACTGAGAGA TATCTTAAAA
    GATGGTGCTG CTTATCATCA TGCTGGTATG GAGCTGTCAG ATAGAAAAGT AGTTGAGGGA
    GCTTTTACTG TTGGAGATTT ACCAGTTCTT TTTACTACCA GTACTTTAGC TATGGGAGTA
    AATTTGCCTG CTCACCTAGT AGTTATAAAA TCTACAATGC ATTATGCTGG AGGACTGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CTACTGCAGT TATCATGACT CGATTAAGCA CAAGGGACAA GTACATTCAG
    ATGTTAGCTT GTAGAGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAACATTTA
    AATGCAGAGA TAGTACTGCA TACCATCACG GATGTGAATA TTGCTGTGGA ATGGATACGA
    TCAACTCTGC TTTATATCAG AGCCTTGAAA AATCCATCTC ATTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA AGCAAAATTA CAAGAATTAT GTTTGAAGAA TCTGAATGAT
    TTATCATCCC TGGACTTAAT AAAGATGGAT GAAGGTGTTA ATTTCAAACC AACTGAAGCA
    GGAAGATTGA TGGCTTGGTA TTATATTACA TTTGAGACAG TGAAGAAATT TTATACAATC
    AGTGGAAAAG AAACCTTATC AGATCTGATT TCCAATGGAA GGAAGAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu103608
    Clone ID Related Accession (Same CDS sequence) XM_024453708.1
    Accession Version XM_024453708.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2427bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product probable ATP-dependent DNA helicase HFM1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA ACCATCCAGA CAATGAAAAG TCATTGGATT GGTTTCTCCC TCCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGAG TTAGAGGAAG AATTAGAAAG TCATAAACTG
    TTAGGTCAGG AAAAGAGGCC AAAAATGTTA ACATCAAATT TAAAGATAAC TAATGAAGAT
    ACAAATTATA TTTCACTAAC ACAAAAATTC CAGTTTGCCT TTCCTTCTGA TAAATATGAA
    CAGGATGATC TAAATTTAGA AGGGGTAGGT AATAATGACT TATCACATAT TGCTGGCAAG
    CTGACATATG CTTCTCAGAA ATATAAAAAT CACATTGGCA CTGAGATAGC ACCTGAGAAG
    AGTGTTCCTG ATGATACAAA ATTAGTTAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GTATTCCGGA AAAGATTATT TAAAATATCT GACAATATAC ATGGGAGTGC TTATTCTAAT
    GACAATGAAT TGGACTCTCA CATTGGCTCA GTGAAAATTG TACAAACAGA AATGAACAAA
    GGGAAATCAA GGAACTATAG CAATAGTAAG CAAAAATTTC AGTATTCTGC AAATGTGTTT
    ACAGCAAATA ATGCTTTTTC TGCTTCTGAA ATCGGAGAAG GCATGTTCAA AGCACCATCT
    TTTTCAGTTG CTTTCCAACC TCATGATATT CAAGAGGTAA CAGAAAATGG TTTAGGTTCC
    TTGAAGGCTG TCACAGAAAT TCCGGCAAAA TTTAGAAGTA TTTTCAAAGA ATTTCCATAT
    TTCAACTATA TACAGTCCAA GGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGAAAAACT GTAGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTTGAAT ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCTTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TTACTGGAGA TACAGTAATG GATGATCTAT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTTCAGC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATATTGTAAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TGTTTCTCAG
    ACTTTAAAAA ATACCAGCAC TGCTATTCCA ATGCGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGACCAGC TGTGTGTCTG
    AAAATGGATG AGAGCCATAG ACCAGTGAAA CTTCAGAAAG TGGTCCTTGG ATTTCCCTGC
    AGTAGTAACC AAACTGAGTT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    ATACAAATGT ACTCTGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAGGGTGTG
    CAACAGGCTG CTTCTGTTCT TGTGAAAGAT GCTAAATTTA TTATGACTGT GGAACAGAAA
    CAGAGGTTAC AGAAGTATGC ATATTCCGTA AGAGATTCAA AACTGAGAGA TATCTTAAAA
    GATGGTGCTG CTTATCATCA TGCTGGTATG GAGCTGTCAG ATAGAAAAGT AGTTGAGGGA
    GCTTTTACTG TTGGAGATTT ACCAGTTCTT TTTACTACCA GTACTTTAGC TATGGGAGTA
    AATTTGCCTG CTCACCTAGT AGTTATAAAA TCTACAATGC ATTATGCTGG AGGACTGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CTACTGCAGT TATCATGACT CGATTAAGCA CAAGGGACAA GTACATTCAG
    ATGTTAGCTT GTAGAGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAACATTTA
    AATGCAGAGA TAGTACTGCA TACCATCACG GATGTGAATA TTGCTGTGGA ATGGATACGA
    TCAACTCTGC TTTATATCAG AGCCTTGAAA AATCCATCTC ATTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA AGCAAAATTA CAAGAATTAT GTTTGAAGAA TCTGAATGAT
    TTATCATCCC TGGACTTAAT AAAGATGGAT GAAGGTGTTA ATTTCAAACC AACTGTATCT
    GCAGGTCACA GGGTGACAGA GGCTGCAGCC AAGTTACCTG AGGCCTCCCG GAAAAAAGGG
    GATGCAGTAC TGGAGACGGG ACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024309476.1
    CDS44..2470
    Translation

    Target ORF information:

    RefSeq Version XM_024453708.1
    Organism Homo sapiens(human)
    Definition Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024453708.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA ACCATCCAGA CAATGAAAAG TCATTGGATT GGTTTCTCCC TCCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGAG TTAGAGGAAG AATTAGAAAG TCATAAACTG
    TTAGGTCAGG AAAAGAGGCC AAAAATGTTA ACATCAAATT TAAAGATAAC TAATGAAGAT
    ACAAATTATA TTTCACTAAC ACAAAAATTC CAGTTTGCCT TTCCTTCTGA TAAATATGAA
    CAGGATGATC TAAATTTAGA AGGGGTAGGT AATAATGACT TATCACATAT TGCTGGCAAG
    CTGACATATG CTTCTCAGAA ATATAAAAAT CACATTGGCA CTGAGATAGC ACCTGAGAAG
    AGTGTTCCTG ATGATACAAA ATTAGTTAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GTATTCCGGA AAAGATTATT TAAAATATCT GACAATATAC ATGGGAGTGC TTATTCTAAT
    GACAATGAAT TGGACTCTCA CATTGGCTCA GTGAAAATTG TACAAACAGA AATGAACAAA
    GGGAAATCAA GGAACTATAG CAATAGTAAG CAAAAATTTC AGTATTCTGC AAATGTGTTT
    ACAGCAAATA ATGCTTTTTC TGCTTCTGAA ATCGGAGAAG GCATGTTCAA AGCACCATCT
    TTTTCAGTTG CTTTCCAACC TCATGATATT CAAGAGGTAA CAGAAAATGG TTTAGGTTCC
    TTGAAGGCTG TCACAGAAAT TCCGGCAAAA TTTAGAAGTA TTTTCAAAGA ATTTCCATAT
    TTCAACTATA TACAGTCCAA GGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGAAAAACT GTAGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTTGAAT ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCTTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TTACTGGAGA TACAGTAATG GATGATCTAT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTTCAGC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATATTGTAAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TGTTTCTCAG
    ACTTTAAAAA ATACCAGCAC TGCTATTCCA ATGCGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGACCAGC TGTGTGTCTG
    AAAATGGATG AGAGCCATAG ACCAGTGAAA CTTCAGAAAG TGGTCCTTGG ATTTCCCTGC
    AGTAGTAACC AAACTGAGTT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    ATACAAATGT ACTCTGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAGGGTGTG
    CAACAGGCTG CTTCTGTTCT TGTGAAAGAT GCTAAATTTA TTATGACTGT GGAACAGAAA
    CAGAGGTTAC AGAAGTATGC ATATTCCGTA AGAGATTCAA AACTGAGAGA TATCTTAAAA
    GATGGTGCTG CTTATCATCA TGCTGGTATG GAGCTGTCAG ATAGAAAAGT AGTTGAGGGA
    GCTTTTACTG TTGGAGATTT ACCAGTTCTT TTTACTACCA GTACTTTAGC TATGGGAGTA
    AATTTGCCTG CTCACCTAGT AGTTATAAAA TCTACAATGC ATTATGCTGG AGGACTGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CTACTGCAGT TATCATGACT CGATTAAGCA CAAGGGACAA GTACATTCAG
    ATGTTAGCTT GTAGAGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAACATTTA
    AATGCAGAGA TAGTACTGCA TACCATCACG GATGTGAATA TTGCTGTGGA ATGGATACGA
    TCAACTCTGC TTTATATCAG AGCCTTGAAA AATCCATCTC ATTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA AGCAAAATTA CAAGAATTAT GTTTGAAGAA TCTGAATGAT
    TTATCATCCC TGGACTTAAT AAAGATGGAT GAAGGTGTTA ATTTCAAACC AACTGTATCT
    GCAGGTCACA GGGTGACAGA GGCTGCAGCC AAGTTACCTG AGGCCTCCCG GAAAAAAGGG
    GATGCAGTAC TGGAGACGGG ACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu83247
    Clone ID Related Accession (Same CDS sequence) XM_017000493.1
    Accession Version XM_017000493.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2637bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product probable ATP-dependent DNA helicase HFM1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGTTGACAT TCGAGTTACA GAAGTATGCA TATTCCGTAA GAGATTCAAA ACTGAGAGAT 
    ATCTTAAAAG ATGGTGCTGC TTATCATCAT GCTGGTATGG AGCTGTCAGA TAGAAAAGTA
    GTTGAGGGAG CTTTTACTGT TGGAGATTTA CCAGTTCTTT TTACTACCAG TACTTTAGCT
    ATGGGAGTAA ATTTGCCTGC TCACCTAGTA GTTATAAAAT CTACAATGCA TTATGCTGGA
    GGACTGTTTG AAGAGTACAG TGAAACAGAT ATTCTACAGA TGATTGGTAG AGCTGGTCGA
    CCTCAATTTG ACACTACAGC TACTGCAGTT ATCATGACTC GATTAAGCAC AAGGGACAAG
    TACATTCAGA TGTTAGCTTG TAGAGACACT GTAGAAAGCA GTTTGCACAG ACATCTTATT
    GAACATTTAA ATGCAGAGAT AGTACTGCAT ACCATCACGG ATGTGAATAT TGCTGTGGAA
    TGGATACGAT CAACTCTGCT TTATATCAGA GCCTTGAAAA ATCCATCTCA TTATGGTTTT
    GCATCTGGAT TGAACAAAGA TGGAATTGAA GCAAAATTAC AAGAATTATG TTTGAAGAAT
    CTGAATGATT TATCATCCCT GGACTTAATA AAGATGGATG AAGGTGTTAA TTTCAAACCA
    ACTGAAGCAG GAAGATTGAT GGCTTGGTAT TATATTACAT TTGAGACAGT GAAGAAATTT
    TATACAATCA GTGGAAAAGA AACCTTATCA GATCTGGTTA CATTGATAGC TGGCTGCAAG
    GAATTTCTAG ATATACAGTT AAGGATAAAT GAAAAGAAAA CACTGAATAC TTTGAACAAA
    GATCCAAATC GGATAACTAT CAGATTTCCA ATGGAAGGAA GAATTAAAAC AAGAGAAATG
    AAAGTAAATT GTCTTATTCA GGCTCAACTA GGATGCATTC CCATACAAGA TTTTGCTTTG
    ACACAAGATA CCGCAAAGAT TTTCAGACAT GGCTCCCGAA TTACAAGATG GTTGTCAGAT
    TTTGTAGCTG CTCAAGAAAA GAAGTTTGCT GTACTATTGA ATAGTTTGAT TTTAGCTAAA
    TGTTTTAGGT GTAAACTTTG GGAAAACTCT CTGCATGTAT CCAAACAACT GGAAAAAATT
    GGTATAACAT TGTCAAATGC AATCGTAAAT GCTGGTTTGA CTTCCTTTAA AAAAATAGAA
    GAGACAGATG CAAGGGAACT TGAATTGATT TTAAACAGAC ATCCCCCCTT TGGAACCCAG
    ATAAAAGAAA CTGTGATGTA TCTACCAAAA TATGAACTTA AAGTGGAACA GATTACAAGA
    TATAGTGATA CGACGGCAGA AATATTAGTG ACTGTTATAT TAAGAAATTT TGAACAGCTA
    CAAACTAAAA GAACAGCATC GGATTCTCAC TATGTTACCT TAATCATAGG TGACGCAGAT
    AATCAAGTAG TTTATCTGCA CAAGATTACG GATTCTGTTT TGCTAAAAGC TGGAAGTTGG
    GCTAAAAAGA TTGCTGTGAA AAGAGCTCTT AAATCTGAAG ATCTTAGCAT AAATCTAATA
    AGTTCTGAAT TTGTTGGGCT TGATATTCAG CAGAAACTTA CAGTCTTTTA CTTAGAACCC
    AAGAGGTTTG GAAATCAAAT CACTATGCAA AGAAAATCTG AAACACAGAT TTCCCATTCT
    AAACATTCAG ACATATCTAC AATAGCAGGA CCTAATAAAG GAACGACTGC CAGCAAAAAA
    CCTGGGAACC GAGAATGCAA TCATCTTTGT AAAAGTAAAC ATACATGTGG ACATGACTGC
    TGTAAAATTG GAGTTGCACA GAAGTCAGAA ATTAAAGAGT CAACAATTTC TTCATATTTA
    TCTGATTTAA GAAACAGGAA TGCTGTTTCA TCTGTTCCTC CAGTTAAACG TCTGAAGATA
    CAGATGAATA AATCTCAAAG TGTGGACCTT AAAGAGTTTG GTTTTACTCC AAAACCTTCC
    CTTCCTAGTA TATCAAGGTC AGAATATTTA AACATATCTG AATTGCCTAT AATGGAGCAG
    TGGGATCAGC CTGAAATCTA TGGAAAAGTT AGACAAGAAC CATCTGAATA TCAAGACAAA
    GAAGTTTTGA ATGTGAACTT TGAATTGGGA AATGAAGTTT GGGATGATTT TGATGATGAA
    AACTTAGAAG TTACTAGCTT TTCAACTGAT ACTGAGAAGA CAAAAATATC AGGATTTGGA
    AACACTTTGA GTTCAAGTAC CAGGGGAAGT AAGCTACCCC TTCAAGAGTC AAAGAGCAAA
    TTCCAAAGAG AAATGTCAAA CAGTTTTGTT TCATCACATG AGATGTCGGA TATTTCTTTA
    TCAAATTCTG CTATGCCCAA GTTCAGTGCA TCCTCCATGA CAAAATTACC TCAACAAGCC
    GGAAATGCAG TTATTGTCCA TTTTCAAGAA AGAAAACCAC AAAATCTGTC ACCAGAGATT
    GAGAAGCAAT GCTTTACTTT CTCTGAAAAA AACCCAAATT CTTCAAATTA TAAAAAAGTG
    GATTTTTTTA TTAGAAACAG TGAATGTAAA AAGGAAGTTG ATTTCAGTAT GTATCATCCT
    GATGATGAAG CTGATGAAAT GAAGTCTTTA TTGGGAATAT TTGATGGTAT TTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016855982.1
    CDS22..2658
    Translation

    Target ORF information:

    RefSeq Version XM_017000493.1
    Organism Homo sapiens(human)
    Definition Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017000493.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGTTGACAT TCGAGTTACA GAAGTATGCA TATTCCGTAA GAGATTCAAA ACTGAGAGAT 
    ATCTTAAAAG ATGGTGCTGC TTATCATCAT GCTGGTATGG AGCTGTCAGA TAGAAAAGTA
    GTTGAGGGAG CTTTTACTGT TGGAGATTTA CCAGTTCTTT TTACTACCAG TACTTTAGCT
    ATGGGAGTAA ATTTGCCTGC TCACCTAGTA GTTATAAAAT CTACAATGCA TTATGCTGGA
    GGACTGTTTG AAGAGTACAG TGAAACAGAT ATTCTACAGA TGATTGGTAG AGCTGGTCGA
    CCTCAATTTG ACACTACAGC TACTGCAGTT ATCATGACTC GATTAAGCAC AAGGGACAAG
    TACATTCAGA TGTTAGCTTG TAGAGACACT GTAGAAAGCA GTTTGCACAG ACATCTTATT
    GAACATTTAA ATGCAGAGAT AGTACTGCAT ACCATCACGG ATGTGAATAT TGCTGTGGAA
    TGGATACGAT CAACTCTGCT TTATATCAGA GCCTTGAAAA ATCCATCTCA TTATGGTTTT
    GCATCTGGAT TGAACAAAGA TGGAATTGAA GCAAAATTAC AAGAATTATG TTTGAAGAAT
    CTGAATGATT TATCATCCCT GGACTTAATA AAGATGGATG AAGGTGTTAA TTTCAAACCA
    ACTGAAGCAG GAAGATTGAT GGCTTGGTAT TATATTACAT TTGAGACAGT GAAGAAATTT
    TATACAATCA GTGGAAAAGA AACCTTATCA GATCTGGTTA CATTGATAGC TGGCTGCAAG
    GAATTTCTAG ATATACAGTT AAGGATAAAT GAAAAGAAAA CACTGAATAC TTTGAACAAA
    GATCCAAATC GGATAACTAT CAGATTTCCA ATGGAAGGAA GAATTAAAAC AAGAGAAATG
    AAAGTAAATT GTCTTATTCA GGCTCAACTA GGATGCATTC CCATACAAGA TTTTGCTTTG
    ACACAAGATA CCGCAAAGAT TTTCAGACAT GGCTCCCGAA TTACAAGATG GTTGTCAGAT
    TTTGTAGCTG CTCAAGAAAA GAAGTTTGCT GTACTATTGA ATAGTTTGAT TTTAGCTAAA
    TGTTTTAGGT GTAAACTTTG GGAAAACTCT CTGCATGTAT CCAAACAACT GGAAAAAATT
    GGTATAACAT TGTCAAATGC AATCGTAAAT GCTGGTTTGA CTTCCTTTAA AAAAATAGAA
    GAGACAGATG CAAGGGAACT TGAATTGATT TTAAACAGAC ATCCCCCCTT TGGAACCCAG
    ATAAAAGAAA CTGTGATGTA TCTACCAAAA TATGAACTTA AAGTGGAACA GATTACAAGA
    TATAGTGATA CGACGGCAGA AATATTAGTG ACTGTTATAT TAAGAAATTT TGAACAGCTA
    CAAACTAAAA GAACAGCATC GGATTCTCAC TATGTTACCT TAATCATAGG TGACGCAGAT
    AATCAAGTAG TTTATCTGCA CAAGATTACG GATTCTGTTT TGCTAAAAGC TGGAAGTTGG
    GCTAAAAAGA TTGCTGTGAA AAGAGCTCTT AAATCTGAAG ATCTTAGCAT AAATCTAATA
    AGTTCTGAAT TTGTTGGGCT TGATATTCAG CAGAAACTTA CAGTCTTTTA CTTAGAACCC
    AAGAGGTTTG GAAATCAAAT CACTATGCAA AGAAAATCTG AAACACAGAT TTCCCATTCT
    AAACATTCAG ACATATCTAC AATAGCAGGA CCTAATAAAG GAACGACTGC CAGCAAAAAA
    CCTGGGAACC GAGAATGCAA TCATCTTTGT AAAAGTAAAC ATACATGTGG ACATGACTGC
    TGTAAAATTG GAGTTGCACA GAAGTCAGAA ATTAAAGAGT CAACAATTTC TTCATATTTA
    TCTGATTTAA GAAACAGGAA TGCTGTTTCA TCTGTTCCTC CAGTTAAACG TCTGAAGATA
    CAGATGAATA AATCTCAAAG TGTGGACCTT AAAGAGTTTG GTTTTACTCC AAAACCTTCC
    CTTCCTAGTA TATCAAGGTC AGAATATTTA AACATATCTG AATTGCCTAT AATGGAGCAG
    TGGGATCAGC CTGAAATCTA TGGAAAAGTT AGACAAGAAC CATCTGAATA TCAAGACAAA
    GAAGTTTTGA ATGTGAACTT TGAATTGGGA AATGAAGTTT GGGATGATTT TGATGATGAA
    AACTTAGAAG TTACTAGCTT TTCAACTGAT ACTGAGAAGA CAAAAATATC AGGATTTGGA
    AACACTTTGA GTTCAAGTAC CAGGGGAAGT AAGCTACCCC TTCAAGAGTC AAAGAGCAAA
    TTCCAAAGAG AAATGTCAAA CAGTTTTGTT TCATCACATG AGATGTCGGA TATTTCTTTA
    TCAAATTCTG CTATGCCCAA GTTCAGTGCA TCCTCCATGA CAAAATTACC TCAACAAGCC
    GGAAATGCAG TTATTGTCCA TTTTCAAGAA AGAAAACCAC AAAATCTGTC ACCAGAGATT
    GAGAAGCAAT GCTTTACTTT CTCTGAAAAA AACCCAAATT CTTCAAATTA TAAAAAAGTG
    GATTTTTTTA TTAGAAACAG TGAATGTAAA AAGGAAGTTG ATTTCAGTAT GTATCATCCT
    GATGATGAAG CTGATGAAAT GAAGTCTTTA TTGGGAATAT TTGATGGTAT TTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu83248
    Clone ID Related Accession (Same CDS sequence) XM_017000494.1
    Accession Version XM_017000494.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2631bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product probable ATP-dependent DNA helicase HFM1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA ACCATCCAGA CAATGAAAAG TCATTGGATT GGTTTCTCCC TCCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGAG TTAGAGGAAG AATTAGAAAG TCATAAACTG
    TTAGGTCAGG AAAAGAGGCC AAAAATGTTA ACATCAAATT TAAAGATAAC TAATGAAGAT
    ACAAATTATA TTTCACTAAC ACAAAAATTC CAGTTTGCCT TTCCTTCTGA TAAATATGAA
    CAGGATGATC TAAATTTAGA AGGGGTAGGT AATAATGACT TATCACATAT TGCTGGCAAG
    CTGACATATG CTTCTCAGAA ATATAAAAAT CACATTGGCA CTGAGATAGC ACCTGAGAAG
    AGTGTTCCTG ATGATACAAA ATTAGTTAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GTATTCCGGA AAAGATTATT TAAAATATCT GACAATATAC ATGGGAGTGC TTATTCTAAT
    GACAATGAAT TGGACTCTCA CATTGGCTCA GTGAAAATTG TACAAACAGA AATGAACAAA
    GGGAAATCAA GGAACTATAG CAATAGTAAG CAAAAATTTC AGTATTCTGC AAATGTGTTT
    ACAGCAAATA ATGCTTTTTC TGCTTCTGAA ATCGGAGAAG GCATGTTCAA AGCACCATCT
    TTTTCAGTTG CTTTCCAACC TCATGATATT CAAGAGGTAA CAGAAAATGG TTTAGGTTCC
    TTGAAGGCTG TCACAGAAAT TCCGGCAAAA TTTAGAAGTA TTTTCAAAGA ATTTCCATAT
    TTCAACTATA TACAGTCCAA GGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGAAAAACT GTAGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTTGAAT ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCTTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TTACTGGAGA TACAGTAATG GATGATCTAT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTTCAGC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATATTGTAAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TGTTTCTCAG
    ACTTTAAAAA ATACCAGCAC TGCTATTCCA ATGCGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGACCAGC TGTGTGTCTG
    AAAATGGATG AGAGCCATAG ACCAGTGAAA CTTCAGAAAG TGGTCCTTGG ATTTCCCTGC
    AGTAGTAACC AAACTGAGTT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    ATACAAATGT ACTCTGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAGGGTGTG
    CAACAGGCTG CTTCTGTTCT TGTGAAAGAT GCTAAATTTA TTATGACTGT GGAACAGAAA
    CAGAGGTTAC AGAAGTATGC ATATTCCGTA AGAGATTCAA AACTGAGAGA TATCTTAAAA
    GATGGTGCTG CTTATCATCA TGCTGGTATG GAGCTGTCAG ATAGAAAAGT AGTTGAGGGA
    GCTTTTACTG TTGGAGATTT ACCAGTTCTT TTTACTACCA GTACTTTAGC TATGGGAGTA
    AATTTGCCTG CTCACCTAGT AGTTATAAAA TCTACAATGC ATTATGCTGG AGGACTGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CTACTGCAGT TATCATGACT CGATTAAGCA CAAGGGACAA GTACATTCAG
    ATGTTAGCTT GTAGAGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAACATTTA
    AATGCAGAGA TAGTACTGCA TACCATCACG GATGTGAATA TTGCTGTGGA ATGGATACGA
    TCAACTCTGC TTTATATCAG AGCCTTGAAA AATCCATCTC ATTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA AGCAAAATTA CAAGAATTAT GTTTGAAGAA TCTGAATGAT
    TTATCATCCC TGGACTTAAT AAAGATGGAT GAAGGTGTTA ATTTCAAACC AACTGAAGCA
    GGAAGATTGA TGGCTTGGTA TTATATTACA TTTGAGACAG TGAAGAAATT TTATACAATC
    AGTGGAAAAG AAACCTTATC AGATCTGGTT ACATTGATAG CTGGCTGCAA GGAATTTCTA
    GATATACAGT TAAGGATAAA TGAAAAGAAA ACACTGAATA CTTTGAACAA AGATCCAAAT
    CGGATAACTA TCAGATTTCC AATGGAAGGA AGAATTAAAA CAAGAGAAAT GAAAGTAAAT
    TGGTATAACA TTGTCAAATG CAATCGTAAA TGCTGGTTTG ACTTCCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016855983.1
    CDS44..2674
    Translation

    Target ORF information:

    RefSeq Version XM_017000494.1
    Organism Homo sapiens(human)
    Definition Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017000494.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA ACCATCCAGA CAATGAAAAG TCATTGGATT GGTTTCTCCC TCCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGAG TTAGAGGAAG AATTAGAAAG TCATAAACTG
    TTAGGTCAGG AAAAGAGGCC AAAAATGTTA ACATCAAATT TAAAGATAAC TAATGAAGAT
    ACAAATTATA TTTCACTAAC ACAAAAATTC CAGTTTGCCT TTCCTTCTGA TAAATATGAA
    CAGGATGATC TAAATTTAGA AGGGGTAGGT AATAATGACT TATCACATAT TGCTGGCAAG
    CTGACATATG CTTCTCAGAA ATATAAAAAT CACATTGGCA CTGAGATAGC ACCTGAGAAG
    AGTGTTCCTG ATGATACAAA ATTAGTTAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GTATTCCGGA AAAGATTATT TAAAATATCT GACAATATAC ATGGGAGTGC TTATTCTAAT
    GACAATGAAT TGGACTCTCA CATTGGCTCA GTGAAAATTG TACAAACAGA AATGAACAAA
    GGGAAATCAA GGAACTATAG CAATAGTAAG CAAAAATTTC AGTATTCTGC AAATGTGTTT
    ACAGCAAATA ATGCTTTTTC TGCTTCTGAA ATCGGAGAAG GCATGTTCAA AGCACCATCT
    TTTTCAGTTG CTTTCCAACC TCATGATATT CAAGAGGTAA CAGAAAATGG TTTAGGTTCC
    TTGAAGGCTG TCACAGAAAT TCCGGCAAAA TTTAGAAGTA TTTTCAAAGA ATTTCCATAT
    TTCAACTATA TACAGTCCAA GGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGAAAAACT GTAGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTTGAAT ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCTTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TTACTGGAGA TACAGTAATG GATGATCTAT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTTCAGC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATATTGTAAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TGTTTCTCAG
    ACTTTAAAAA ATACCAGCAC TGCTATTCCA ATGCGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGACCAGC TGTGTGTCTG
    AAAATGGATG AGAGCCATAG ACCAGTGAAA CTTCAGAAAG TGGTCCTTGG ATTTCCCTGC
    AGTAGTAACC AAACTGAGTT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    ATACAAATGT ACTCTGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAGGGTGTG
    CAACAGGCTG CTTCTGTTCT TGTGAAAGAT GCTAAATTTA TTATGACTGT GGAACAGAAA
    CAGAGGTTAC AGAAGTATGC ATATTCCGTA AGAGATTCAA AACTGAGAGA TATCTTAAAA
    GATGGTGCTG CTTATCATCA TGCTGGTATG GAGCTGTCAG ATAGAAAAGT AGTTGAGGGA
    GCTTTTACTG TTGGAGATTT ACCAGTTCTT TTTACTACCA GTACTTTAGC TATGGGAGTA
    AATTTGCCTG CTCACCTAGT AGTTATAAAA TCTACAATGC ATTATGCTGG AGGACTGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CTACTGCAGT TATCATGACT CGATTAAGCA CAAGGGACAA GTACATTCAG
    ATGTTAGCTT GTAGAGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAACATTTA
    AATGCAGAGA TAGTACTGCA TACCATCACG GATGTGAATA TTGCTGTGGA ATGGATACGA
    TCAACTCTGC TTTATATCAG AGCCTTGAAA AATCCATCTC ATTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA AGCAAAATTA CAAGAATTAT GTTTGAAGAA TCTGAATGAT
    TTATCATCCC TGGACTTAAT AAAGATGGAT GAAGGTGTTA ATTTCAAACC AACTGAAGCA
    GGAAGATTGA TGGCTTGGTA TTATATTACA TTTGAGACAG TGAAGAAATT TTATACAATC
    AGTGGAAAAG AAACCTTATC AGATCTGGTT ACATTGATAG CTGGCTGCAA GGAATTTCTA
    GATATACAGT TAAGGATAAA TGAAAAGAAA ACACTGAATA CTTTGAACAA AGATCCAAAT
    CGGATAACTA TCAGATTTCC AATGGAAGGA AGAATTAAAA CAAGAGAAAT GAAAGTAAAT
    TGGTATAACA TTGTCAAATG CAATCGTAAA TGCTGGTTTG ACTTCCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu77239
    Clone ID Related Accession (Same CDS sequence) XM_017000492.1
    Accession Version XM_017000492.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3345bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product probable ATP-dependent DNA helicase HFM1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAAGTAC CATTGCCATG GTTGAATATT AAAATTGTTT ACATGGCACC AATAAAAGCC 
    TTGTGCAGTC AGCGTTTTGA TGACTGGAAA GAAAAATTTG GACCAATAGG ATTGAATTGT
    AAAGAACTTA CTGGAGATAC AGTAATGGAT GATCTATTTG AGATTCAGCA TGCCCATATT
    ATTATGACAA CTCCAGAAAA ATGGGATAGC ATGACTAGGA AATGGAGAGA CAACTCTTTG
    GTTCAGCTGG TTCGACTGTT TCTCATTGAT GAGGTACATA TTGTAAAAGA TGAAAATCGT
    GGTCCAACTC TTGAAGTTGT AGTTAGCAGA ATGAAAACTG TACAGTCTGT TTCTCAGACT
    TTAAAAAATA CCAGCACTGC TATTCCAATG CGATTTGTAG CTGTATCTGC AACAATTCCA
    AATGCTGAGG ATATTGCAGA ATGGCTTTCA GATGGTGAAA GACCAGCTGT GTGTCTGAAA
    ATGGATGAGA GCCATAGACC AGTGAAACTT CAGAAAGTGG TCCTTGGATT TCCCTGCAGT
    AGTAACCAAA CTGAGTTTAA GTTTGATTTA ACCCTCAACT ACAAAATTGC CAGTGTTATA
    CAAATGTACT CTGATCAGAA ACCCACACTT GTGTTTTGTG CAACAAGGAA GGGTGTGCAA
    CAGGCTGCTT CTGTTCTTGT GAAAGATGCT AAATTTATTA TGACTGTGGA ACAGAAACAG
    AGGTTACAGA AGTATGCATA TTCCGTAAGA GATTCAAAAC TGAGAGATAT CTTAAAAGAT
    GGTGCTGCTT ATCATCATGC TGGTATGGAG CTGTCAGATA GAAAAGTAGT TGAGGGAGCT
    TTTACTGTTG GAGATTTACC AGTTCTTTTT ACTACCAGTA CTTTAGCTAT GGGAGTAAAT
    TTGCCTGCTC ACCTAGTAGT TATAAAATCT ACAATGCATT ATGCTGGAGG ACTGTTTGAA
    GAGTACAGTG AAACAGATAT TCTACAGATG ATTGGTAGAG CTGGTCGACC TCAATTTGAC
    ACTACAGCTA CTGCAGTTAT CATGACTCGA TTAAGCACAA GGGACAAGTA CATTCAGATG
    TTAGCTTGTA GAGACACTGT AGAAAGCAGT TTGCACAGAC ATCTTATTGA ACATTTAAAT
    GCAGAGATAG TACTGCATAC CATCACGGAT GTGAATATTG CTGTGGAATG GATACGATCA
    ACTCTGCTTT ATATCAGAGC CTTGAAAAAT CCATCTCATT ATGGTTTTGC ATCTGGATTG
    AACAAAGATG GAATTGAAGC AAAATTACAA GAATTATGTT TGAAGAATCT GAATGATTTA
    TCATCCCTGG ACTTAATAAA GATGGATGAA GGTGTTAATT TCAAACCAAC TGAAGCAGGA
    AGATTGATGG CTTGGTATTA TATTACATTT GAGACAGTGA AGAAATTTTA TACAATCAGT
    GGAAAAGAAA CCTTATCAGA TCTGGTTACA TTGATAGCTG GCTGCAAGGA ATTTCTAGAT
    ATACAGTTAA GGATAAATGA AAAGAAAACA CTGAATACTT TGAACAAAGA TCCAAATCGG
    ATAACTATCA GATTTCCAAT GGAAGGAAGA ATTAAAACAA GAGAAATGAA AGTAAATTGT
    CTTATTCAGG CTCAACTAGG ATGCATTCCC ATACAAGATT TTGCTTTGAC ACAAGATACC
    GCAAAGATTT TCAGACATGG CTCCCGAATT ACAAGATGGT TGTCAGATTT TGTAGCTGCT
    CAAGAAAAGA AGTTTGCTGT ACTATTGAAT AGTTTGATTT TAGCTAAATG TTTTAGGTGT
    AAACTTTGGG AAAACTCTCT GCATGTATCC AAACAACTGG AAAAAATTGG TATAACATTG
    TCAAATGCAA TCGTAAATGC TGGTTTGACT TCCTTTAAAA AAATAGAAGA GACAGATGCA
    AGGGAACTTG AATTGATTTT AAACAGACAT CCCCCCTTTG GAACCCAGAT AAAAGAAACT
    GTGATGTATC TACCAAAATA TGAACTTAAA GTGGAACAGA TTACAAGATA TAGTGATACG
    ACGGCAGAAA TATTAGTGAC TGTTATATTA AGAAATTTTG AACAGCTACA AACTAAAAGA
    ACAGCATCGG ATTCTCACTA TGTTACCTTA ATCATAGGTG ACGCAGATAA TCAAGTAGTT
    TATCTGCACA AGATTACGGA TTCTGTTTTG CTAAAAGCTG GAAGTTGGGC TAAAAAGATT
    GCTGTGAAAA GAGCTCTTAA ATCTGAAGAT CTTAGCATAA ATCTAATAAG TTCTGAATTT
    GTTGGGCTTG ATATTCAGCA GAAACTTACA GTCTTTTACT TAGAACCCAA GAGGTTTGGA
    AATCAAATCA CTATGCAAAG AAAATCTGAA ACACAGATTT CCCATTCTAA ACATTCAGAC
    ATATCTACAA TAGCAGGACC TAATAAAGGA ACGACTGCCA GCAAAAAACC TGGGAACCGA
    GAATGCAATC ATCTTTGTAA AAGTAAACAT ACATGTGGAC ATGACTGCTG TAAAATTGGA
    GTTGCACAGA AGTCAGAAAT TAAAGAGTCA ACAATTTCTT CATATTTATC TGATTTAAGA
    AACAGGAATG CTGTTTCATC TGTTCCTCCA GTTAAACGTC TGAAGATACA GATGAATAAA
    TCTCAAAGTG TGGACCTTAA AGAGTTTGGT TTTACTCCAA AACCTTCCCT TCCTAGTATA
    TCAAGGTCAG AATATTTAAA CATATCTGAA TTGCCTATAA TGGAGCAGTG GGATCAGCCT
    GAAATCTATG GAAAAGTTAG ACAAGAACCA TCTGAATATC AAGACAAAGA AGTTTTGAAT
    GTGAACTTTG AATTGGGAAA TGAAGTTTGG GATGATTTTG ATGATGAAAA CTTAGAAGTT
    ACTAGCTTTT CAACTGATAC TGAGAAGACA AAAATATCAG GATTTGGAAA CACTTTGAGT
    TCAAGTACCA GGGGAAGTAA GCTACCCCTT CAAGAGTCAA AGAGCAAATT CCAAAGAGAA
    ATGTCAAACA GTTTTGTTTC ATCACATGAG ATGTCGGATA TTTCTTTATC AAATTCTGCT
    ATGCCCAAGT TCAGTGCATC CTCCATGACA AAATTACCTC AACAAGCCGG AAATGCAGTT
    ATTGTCCATT TTCAAGAAAG AAAACCACAA AATCTGTCAC CAGAGATTGA GAAGCAATGC
    TTTACTTTCT CTGAAAAAAA CCCAAATTCT TCAAATTATA AAAAAGTGGA TTTTTTTATT
    AGAAACAGTG AATGTAAAAA GGAAGTTGAT TTCAGTATGT ATCATCCTGA TGATGAAGCT
    GATGAAATGA AGTCTTTATT GGGAATATTT GATGGTATTT TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016855981.1
    CDS110..3454
    Translation

    Target ORF information:

    RefSeq Version XM_017000492.1
    Organism Homo sapiens(human)
    Definition Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017000492.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAAGTAC CATTGCCATG GTTGAATATT AAAATTGTTT ACATGGCACC AATAAAAGCC 
    TTGTGCAGTC AGCGTTTTGA TGACTGGAAA GAAAAATTTG GACCAATAGG ATTGAATTGT
    AAAGAACTTA CTGGAGATAC AGTAATGGAT GATCTATTTG AGATTCAGCA TGCCCATATT
    ATTATGACAA CTCCAGAAAA ATGGGATAGC ATGACTAGGA AATGGAGAGA CAACTCTTTG
    GTTCAGCTGG TTCGACTGTT TCTCATTGAT GAGGTACATA TTGTAAAAGA TGAAAATCGT
    GGTCCAACTC TTGAAGTTGT AGTTAGCAGA ATGAAAACTG TACAGTCTGT TTCTCAGACT
    TTAAAAAATA CCAGCACTGC TATTCCAATG CGATTTGTAG CTGTATCTGC AACAATTCCA
    AATGCTGAGG ATATTGCAGA ATGGCTTTCA GATGGTGAAA GACCAGCTGT GTGTCTGAAA
    ATGGATGAGA GCCATAGACC AGTGAAACTT CAGAAAGTGG TCCTTGGATT TCCCTGCAGT
    AGTAACCAAA CTGAGTTTAA GTTTGATTTA ACCCTCAACT ACAAAATTGC CAGTGTTATA
    CAAATGTACT CTGATCAGAA ACCCACACTT GTGTTTTGTG CAACAAGGAA GGGTGTGCAA
    CAGGCTGCTT CTGTTCTTGT GAAAGATGCT AAATTTATTA TGACTGTGGA ACAGAAACAG
    AGGTTACAGA AGTATGCATA TTCCGTAAGA GATTCAAAAC TGAGAGATAT CTTAAAAGAT
    GGTGCTGCTT ATCATCATGC TGGTATGGAG CTGTCAGATA GAAAAGTAGT TGAGGGAGCT
    TTTACTGTTG GAGATTTACC AGTTCTTTTT ACTACCAGTA CTTTAGCTAT GGGAGTAAAT
    TTGCCTGCTC ACCTAGTAGT TATAAAATCT ACAATGCATT ATGCTGGAGG ACTGTTTGAA
    GAGTACAGTG AAACAGATAT TCTACAGATG ATTGGTAGAG CTGGTCGACC TCAATTTGAC
    ACTACAGCTA CTGCAGTTAT CATGACTCGA TTAAGCACAA GGGACAAGTA CATTCAGATG
    TTAGCTTGTA GAGACACTGT AGAAAGCAGT TTGCACAGAC ATCTTATTGA ACATTTAAAT
    GCAGAGATAG TACTGCATAC CATCACGGAT GTGAATATTG CTGTGGAATG GATACGATCA
    ACTCTGCTTT ATATCAGAGC CTTGAAAAAT CCATCTCATT ATGGTTTTGC ATCTGGATTG
    AACAAAGATG GAATTGAAGC AAAATTACAA GAATTATGTT TGAAGAATCT GAATGATTTA
    TCATCCCTGG ACTTAATAAA GATGGATGAA GGTGTTAATT TCAAACCAAC TGAAGCAGGA
    AGATTGATGG CTTGGTATTA TATTACATTT GAGACAGTGA AGAAATTTTA TACAATCAGT
    GGAAAAGAAA CCTTATCAGA TCTGGTTACA TTGATAGCTG GCTGCAAGGA ATTTCTAGAT
    ATACAGTTAA GGATAAATGA AAAGAAAACA CTGAATACTT TGAACAAAGA TCCAAATCGG
    ATAACTATCA GATTTCCAAT GGAAGGAAGA ATTAAAACAA GAGAAATGAA AGTAAATTGT
    CTTATTCAGG CTCAACTAGG ATGCATTCCC ATACAAGATT TTGCTTTGAC ACAAGATACC
    GCAAAGATTT TCAGACATGG CTCCCGAATT ACAAGATGGT TGTCAGATTT TGTAGCTGCT
    CAAGAAAAGA AGTTTGCTGT ACTATTGAAT AGTTTGATTT TAGCTAAATG TTTTAGGTGT
    AAACTTTGGG AAAACTCTCT GCATGTATCC AAACAACTGG AAAAAATTGG TATAACATTG
    TCAAATGCAA TCGTAAATGC TGGTTTGACT TCCTTTAAAA AAATAGAAGA GACAGATGCA
    AGGGAACTTG AATTGATTTT AAACAGACAT CCCCCCTTTG GAACCCAGAT AAAAGAAACT
    GTGATGTATC TACCAAAATA TGAACTTAAA GTGGAACAGA TTACAAGATA TAGTGATACG
    ACGGCAGAAA TATTAGTGAC TGTTATATTA AGAAATTTTG AACAGCTACA AACTAAAAGA
    ACAGCATCGG ATTCTCACTA TGTTACCTTA ATCATAGGTG ACGCAGATAA TCAAGTAGTT
    TATCTGCACA AGATTACGGA TTCTGTTTTG CTAAAAGCTG GAAGTTGGGC TAAAAAGATT
    GCTGTGAAAA GAGCTCTTAA ATCTGAAGAT CTTAGCATAA ATCTAATAAG TTCTGAATTT
    GTTGGGCTTG ATATTCAGCA GAAACTTACA GTCTTTTACT TAGAACCCAA GAGGTTTGGA
    AATCAAATCA CTATGCAAAG AAAATCTGAA ACACAGATTT CCCATTCTAA ACATTCAGAC
    ATATCTACAA TAGCAGGACC TAATAAAGGA ACGACTGCCA GCAAAAAACC TGGGAACCGA
    GAATGCAATC ATCTTTGTAA AAGTAAACAT ACATGTGGAC ATGACTGCTG TAAAATTGGA
    GTTGCACAGA AGTCAGAAAT TAAAGAGTCA ACAATTTCTT CATATTTATC TGATTTAAGA
    AACAGGAATG CTGTTTCATC TGTTCCTCCA GTTAAACGTC TGAAGATACA GATGAATAAA
    TCTCAAAGTG TGGACCTTAA AGAGTTTGGT TTTACTCCAA AACCTTCCCT TCCTAGTATA
    TCAAGGTCAG AATATTTAAA CATATCTGAA TTGCCTATAA TGGAGCAGTG GGATCAGCCT
    GAAATCTATG GAAAAGTTAG ACAAGAACCA TCTGAATATC AAGACAAAGA AGTTTTGAAT
    GTGAACTTTG AATTGGGAAA TGAAGTTTGG GATGATTTTG ATGATGAAAA CTTAGAAGTT
    ACTAGCTTTT CAACTGATAC TGAGAAGACA AAAATATCAG GATTTGGAAA CACTTTGAGT
    TCAAGTACCA GGGGAAGTAA GCTACCCCTT CAAGAGTCAA AGAGCAAATT CCAAAGAGAA
    ATGTCAAACA GTTTTGTTTC ATCACATGAG ATGTCGGATA TTTCTTTATC AAATTCTGCT
    ATGCCCAAGT TCAGTGCATC CTCCATGACA AAATTACCTC AACAAGCCGG AAATGCAGTT
    ATTGTCCATT TTCAAGAAAG AAAACCACAA AATCTGTCAC CAGAGATTGA GAAGCAATGC
    TTTACTTTCT CTGAAAAAAA CCCAAATTCT TCAAATTATA AAAAAGTGGA TTTTTTTATT
    AGAAACAGTG AATGTAAAAA GGAAGTTGAT TTCAGTATGT ATCATCCTGA TGATGAAGCT
    GATGAAATGA AGTCTTTATT GGGAATATTT GATGGTATTT TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu77240
    Clone ID Related Accession (Same CDS sequence) XM_011540859.2
    Accession Version XM_011540859.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3135bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product probable ATP-dependent DNA helicase HFM1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540859.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACTAGGA AATGGAGAGA CAACTCTTTG GTTCAGCTGG TTCGACTGTT TCTCATTGAT 
    GAGGTACATA TTGTAAAAGA TGAAAATCGT GGTCCAACTC TTGAAGTTGT AGTTAGCAGA
    ATGAAAACTG TACAGTCTGT TTCTCAGACT TTAAAAAATA CCAGCACTGC TATTCCAATG
    CGATTTGTAG CTGTATCTGC AACAATTCCA AATGCTGAGG ATATTGCAGA ATGGCTTTCA
    GATGGTGAAA GACCAGCTGT GTGTCTGAAA ATGGATGAGA GCCATAGACC AGTGAAACTT
    CAGAAAGTGG TCCTTGGATT TCCCTGCAGT AGTAACCAAA CTGAGTTTAA GTTTGATTTA
    ACCCTCAACT ACAAAATTGC CAGTGTTATA CAAATGTACT CTGATCAGAA ACCCACACTT
    GTGTTTTGTG CAACAAGGAA GGGTGTGCAA CAGGCTGCTT CTGTTCTTGT GAAAGATGCT
    AAATTTATTA TGACTGTGGA ACAGAAACAG AGGTTACAGA AGTATGCATA TTCCGTAAGA
    GATTCAAAAC TGAGAGATAT CTTAAAAGAT GGTGCTGCTT ATCATCATGC TGGTATGGAG
    CTGTCAGATA GAAAAGTAGT TGAGGGAGCT TTTACTGTTG GAGATTTACC AGTTCTTTTT
    ACTACCAGTA CTTTAGCTAT GGGAGTAAAT TTGCCTGCTC ACCTAGTAGT TATAAAATCT
    ACAATGCATT ATGCTGGAGG ACTGTTTGAA GAGTACAGTG AAACAGATAT TCTACAGATG
    ATTGGTAGAG CTGGTCGACC TCAATTTGAC ACTACAGCTA CTGCAGTTAT CATGACTCGA
    TTAAGCACAA GGGACAAGTA CATTCAGATG TTAGCTTGTA GAGACACTGT AGAAAGCAGT
    TTGCACAGAC ATCTTATTGA ACATTTAAAT GCAGAGATAG TACTGCATAC CATCACGGAT
    GTGAATATTG CTGTGGAATG GATACGATCA ACTCTGCTTT ATATCAGAGC CTTGAAAAAT
    CCATCTCATT ATGGTTTTGC ATCTGGATTG AACAAAGATG GAATTGAAGC AAAATTACAA
    GAATTATGTT TGAAGAATCT GAATGATTTA TCATCCCTGG ACTTAATAAA GATGGATGAA
    GGTGTTAATT TCAAACCAAC TGAAGCAGGA AGATTGATGG CTTGGTATTA TATTACATTT
    GAGACAGTGA AGAAATTTTA TACAATCAGT GGAAAAGAAA CCTTATCAGA TCTGGTTACA
    TTGATAGCTG GCTGCAAGGA ATTTCTAGAT ATACAGTTAA GGATAAATGA AAAGAAAACA
    CTGAATACTT TGAACAAAGA TCCAAATCGG ATAACTATCA GATTTCCAAT GGAAGGAAGA
    ATTAAAACAA GAGAAATGAA AGTAAATTGT CTTATTCAGG CTCAACTAGG ATGCATTCCC
    ATACAAGATT TTGCTTTGAC ACAAGATACC GCAAAGATTT TCAGACATGG CTCCCGAATT
    ACAAGATGGT TGTCAGATTT TGTAGCTGCT CAAGAAAAGA AGTTTGCTGT ACTATTGAAT
    AGTTTGATTT TAGCTAAATG TTTTAGGTGT AAACTTTGGG AAAACTCTCT GCATGTATCC
    AAACAACTGG AAAAAATTGG TATAACATTG TCAAATGCAA TCGTAAATGC TGGTTTGACT
    TCCTTTAAAA AAATAGAAGA GACAGATGCA AGGGAACTTG AATTGATTTT AAACAGACAT
    CCCCCCTTTG GAACCCAGAT AAAAGAAACT GTGATGTATC TACCAAAATA TGAACTTAAA
    GTGGAACAGA TTACAAGATA TAGTGATACG ACGGCAGAAA TATTAGTGAC TGTTATATTA
    AGAAATTTTG AACAGCTACA AACTAAAAGA ACAGCATCGG ATTCTCACTA TGTTACCTTA
    ATCATAGGTG ACGCAGATAA TCAAGTAGTT TATCTGCACA AGATTACGGA TTCTGTTTTG
    CTAAAAGCTG GAAGTTGGGC TAAAAAGATT GCTGTGAAAA GAGCTCTTAA ATCTGAAGAT
    CTTAGCATAA ATCTAATAAG TTCTGAATTT GTTGGGCTTG ATATTCAGCA GAAACTTACA
    GTCTTTTACT TAGAACCCAA GAGGTTTGGA AATCAAATCA CTATGCAAAG AAAATCTGAA
    ACACAGATTT CCCATTCTAA ACATTCAGAC ATATCTACAA TAGCAGGACC TAATAAAGGA
    ACGACTGCCA GCAAAAAACC TGGGAACCGA GAATGCAATC ATCTTTGTAA AAGTAAACAT
    ACATGTGGAC ATGACTGCTG TAAAATTGGA GTTGCACAGA AGTCAGAAAT TAAAGAGTCA
    ACAATTTCTT CATATTTATC TGATTTAAGA AACAGGAATG CTGTTTCATC TGTTCCTCCA
    GTTAAACGTC TGAAGATACA GATGAATAAA TCTCAAAGTG TGGACCTTAA AGAGTTTGGT
    TTTACTCCAA AACCTTCCCT TCCTAGTATA TCAAGGTCAG AATATTTAAA CATATCTGAA
    TTGCCTATAA TGGAGCAGTG GGATCAGCCT GAAATCTATG GAAAAGTTAG ACAAGAACCA
    TCTGAATATC AAGACAAAGA AGTTTTGAAT GTGAACTTTG AATTGGGAAA TGAAGTTTGG
    GATGATTTTG ATGATGAAAA CTTAGAAGTT ACTAGCTTTT CAACTGATAC TGAGAAGACA
    AAAATATCAG GATTTGGAAA CACTTTGAGT TCAAGTACCA GGGGAAGTAA GCTACCCCTT
    CAAGAGTCAA AGAGCAAATT CCAAAGAGAA ATGTCAAACA GTTTTGTTTC ATCACATGAG
    ATGTCGGATA TTTCTTTATC AAATTCTGCT ATGCCCAAGT TCAGTGCATC CTCCATGACA
    AAATTACCTC AACAAGCCGG AAATGCAGTT ATTGTCCATT TTCAAGAAAG AAAACCACAA
    AATCTGTCAC CAGAGATTGA GAAGCAATGC TTTACTTTCT CTGAAAAAAA CCCAAATTCT
    TCAAATTATA AAAAAGTGGA TTTTTTTATT AGAAACAGTG AATGTAAAAA GGAAGTTGAT
    TTCAGTATGT ATCATCCTGA TGATGAAGCT GATGAAATGA AGTCTTTATT GGGAATATTT
    GATGGTATTT TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011539161.1
    CDS806..3940
    Translation

    Target ORF information:

    RefSeq Version XM_011540859.2
    Organism Homo sapiens(human)
    Definition Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011540859.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACTAGGA AATGGAGAGA CAACTCTTTG GTTCAGCTGG TTCGACTGTT TCTCATTGAT 
    GAGGTACATA TTGTAAAAGA TGAAAATCGT GGTCCAACTC TTGAAGTTGT AGTTAGCAGA
    ATGAAAACTG TACAGTCTGT TTCTCAGACT TTAAAAAATA CCAGCACTGC TATTCCAATG
    CGATTTGTAG CTGTATCTGC AACAATTCCA AATGCTGAGG ATATTGCAGA ATGGCTTTCA
    GATGGTGAAA GACCAGCTGT GTGTCTGAAA ATGGATGAGA GCCATAGACC AGTGAAACTT
    CAGAAAGTGG TCCTTGGATT TCCCTGCAGT AGTAACCAAA CTGAGTTTAA GTTTGATTTA
    ACCCTCAACT ACAAAATTGC CAGTGTTATA CAAATGTACT CTGATCAGAA ACCCACACTT
    GTGTTTTGTG CAACAAGGAA GGGTGTGCAA CAGGCTGCTT CTGTTCTTGT GAAAGATGCT
    AAATTTATTA TGACTGTGGA ACAGAAACAG AGGTTACAGA AGTATGCATA TTCCGTAAGA
    GATTCAAAAC TGAGAGATAT CTTAAAAGAT GGTGCTGCTT ATCATCATGC TGGTATGGAG
    CTGTCAGATA GAAAAGTAGT TGAGGGAGCT TTTACTGTTG GAGATTTACC AGTTCTTTTT
    ACTACCAGTA CTTTAGCTAT GGGAGTAAAT TTGCCTGCTC ACCTAGTAGT TATAAAATCT
    ACAATGCATT ATGCTGGAGG ACTGTTTGAA GAGTACAGTG AAACAGATAT TCTACAGATG
    ATTGGTAGAG CTGGTCGACC TCAATTTGAC ACTACAGCTA CTGCAGTTAT CATGACTCGA
    TTAAGCACAA GGGACAAGTA CATTCAGATG TTAGCTTGTA GAGACACTGT AGAAAGCAGT
    TTGCACAGAC ATCTTATTGA ACATTTAAAT GCAGAGATAG TACTGCATAC CATCACGGAT
    GTGAATATTG CTGTGGAATG GATACGATCA ACTCTGCTTT ATATCAGAGC CTTGAAAAAT
    CCATCTCATT ATGGTTTTGC ATCTGGATTG AACAAAGATG GAATTGAAGC AAAATTACAA
    GAATTATGTT TGAAGAATCT GAATGATTTA TCATCCCTGG ACTTAATAAA GATGGATGAA
    GGTGTTAATT TCAAACCAAC TGAAGCAGGA AGATTGATGG CTTGGTATTA TATTACATTT
    GAGACAGTGA AGAAATTTTA TACAATCAGT GGAAAAGAAA CCTTATCAGA TCTGGTTACA
    TTGATAGCTG GCTGCAAGGA ATTTCTAGAT ATACAGTTAA GGATAAATGA AAAGAAAACA
    CTGAATACTT TGAACAAAGA TCCAAATCGG ATAACTATCA GATTTCCAAT GGAAGGAAGA
    ATTAAAACAA GAGAAATGAA AGTAAATTGT CTTATTCAGG CTCAACTAGG ATGCATTCCC
    ATACAAGATT TTGCTTTGAC ACAAGATACC GCAAAGATTT TCAGACATGG CTCCCGAATT
    ACAAGATGGT TGTCAGATTT TGTAGCTGCT CAAGAAAAGA AGTTTGCTGT ACTATTGAAT
    AGTTTGATTT TAGCTAAATG TTTTAGGTGT AAACTTTGGG AAAACTCTCT GCATGTATCC
    AAACAACTGG AAAAAATTGG TATAACATTG TCAAATGCAA TCGTAAATGC TGGTTTGACT
    TCCTTTAAAA AAATAGAAGA GACAGATGCA AGGGAACTTG AATTGATTTT AAACAGACAT
    CCCCCCTTTG GAACCCAGAT AAAAGAAACT GTGATGTATC TACCAAAATA TGAACTTAAA
    GTGGAACAGA TTACAAGATA TAGTGATACG ACGGCAGAAA TATTAGTGAC TGTTATATTA
    AGAAATTTTG AACAGCTACA AACTAAAAGA ACAGCATCGG ATTCTCACTA TGTTACCTTA
    ATCATAGGTG ACGCAGATAA TCAAGTAGTT TATCTGCACA AGATTACGGA TTCTGTTTTG
    CTAAAAGCTG GAAGTTGGGC TAAAAAGATT GCTGTGAAAA GAGCTCTTAA ATCTGAAGAT
    CTTAGCATAA ATCTAATAAG TTCTGAATTT GTTGGGCTTG ATATTCAGCA GAAACTTACA
    GTCTTTTACT TAGAACCCAA GAGGTTTGGA AATCAAATCA CTATGCAAAG AAAATCTGAA
    ACACAGATTT CCCATTCTAA ACATTCAGAC ATATCTACAA TAGCAGGACC TAATAAAGGA
    ACGACTGCCA GCAAAAAACC TGGGAACCGA GAATGCAATC ATCTTTGTAA AAGTAAACAT
    ACATGTGGAC ATGACTGCTG TAAAATTGGA GTTGCACAGA AGTCAGAAAT TAAAGAGTCA
    ACAATTTCTT CATATTTATC TGATTTAAGA AACAGGAATG CTGTTTCATC TGTTCCTCCA
    GTTAAACGTC TGAAGATACA GATGAATAAA TCTCAAAGTG TGGACCTTAA AGAGTTTGGT
    TTTACTCCAA AACCTTCCCT TCCTAGTATA TCAAGGTCAG AATATTTAAA CATATCTGAA
    TTGCCTATAA TGGAGCAGTG GGATCAGCCT GAAATCTATG GAAAAGTTAG ACAAGAACCA
    TCTGAATATC AAGACAAAGA AGTTTTGAAT GTGAACTTTG AATTGGGAAA TGAAGTTTGG
    GATGATTTTG ATGATGAAAA CTTAGAAGTT ACTAGCTTTT CAACTGATAC TGAGAAGACA
    AAAATATCAG GATTTGGAAA CACTTTGAGT TCAAGTACCA GGGGAAGTAA GCTACCCCTT
    CAAGAGTCAA AGAGCAAATT CCAAAGAGAA ATGTCAAACA GTTTTGTTTC ATCACATGAG
    ATGTCGGATA TTTCTTTATC AAATTCTGCT ATGCCCAAGT TCAGTGCATC CTCCATGACA
    AAATTACCTC AACAAGCCGG AAATGCAGTT ATTGTCCATT TTCAAGAAAG AAAACCACAA
    AATCTGTCAC CAGAGATTGA GAAGCAATGC TTTACTTTCT CTGAAAAAAA CCCAAATTCT
    TCAAATTATA AAAAAGTGGA TTTTTTTATT AGAAACAGTG AATGTAAAAA GGAAGTTGAT
    TTCAGTATGT ATCATCCTGA TGATGAAGCT GATGAAATGA AGTCTTTATT GGGAATATTT
    GATGGTATTT TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu77238
    Clone ID Related Accession (Same CDS sequence) XM_011540857.1
    Accession Version XM_011540857.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product probable ATP-dependent DNA helicase HFM1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA AATTATTTAA AATATCTGAC AATATACATG GGAGTGCTTA TTCTAATGAC
    AATGAATTGG ACTCTCACAT TGGCTCAGTG AAAATTGTAC AAACAGAAAT GAACAAAGGG
    AAATCAAGGA ACTATAGCAA TAGTAAGCAA AAATTTCAGT ATTCTGCAAA TGTGTTTACA
    GCAAATAATG CTTTTTCTGC TTCTGAAATC GGAGAAGGCA TGTTCAAAGC ACCATCTTTT
    TCAGTTGCTT TCCAACCTCA TGATATTCAA GAGGTAACAG AAAATGGTTT AGGTTCCTTG
    AAGGCTGTCA CAGAAATTCC GGCAAAATTT AGAAGTATTT TCAAAGAATT TCCATATTTC
    AACTATATAC AGTCCAAGGC CTTTGATGAT CTTCTTTACA CAGATAGGAA TTTTGTGATT
    TGTGCTCCAA CTGGTTCTGG AAAAACTGTA GTGTTTGAAC TAGCTATAAC AAGATTGTTA
    ATGGAAGTAC CATTGCCATG GTTGAATATT AAAATTGTTT ACATGGCACC AATAAAAGCC
    TTGTGCAGTC AGCGTTTTGA TGACTGGAAA GAAAAATTTG GACCAATAGG ATTGAATTGT
    AAAGAACTTA CTGGAGATAC AGTAATGGAT GATCTATTTG AGATTCAGCA TGCCCATATT
    ATTATGACAA CTCCAGAAAA ATGGGATAGC ATGACTAGGA AATGGAGAGA CAACTCTTTG
    GTTCAGCTGG TTCGACTGTT TCTCATTGAT GAGGTACATA TTGTAAAAGA TGAAAATCGT
    GGTCCAACTC TTGAAGTTGT AGTTAGCAGA ATGAAAACTG TACAGTCTGT TTCTCAGACT
    TTAAAAAATA CCAGCACTGC TATTCCAATG CGATTTGTAG CTGTATCTGC AACAATTCCA
    AATGCTGAGG ATATTGCAGA ATGGCTTTCA GATGGTGAAA GACCAGCTGT GTGTCTGAAA
    ATGGATGAGA GCCATAGACC AGTGAAACTT CAGAAAGTGG TCCTTGGATT TCCCTGCAGT
    AGTAACCAAA CTGAGTTTAA GTTTGATTTA ACCCTCAACT ACAAAATTGC CAGTGTTATA
    CAAATGTACT CTGATCAGAA ACCCACACTT GTGTTTTGTG CAACAAGGAA GGGTGTGCAA
    CAGGCTGCTT CTGTTCTTGT GAAAGATGCT AAATTTATTA TGACTGTGGA ACAGAAACAG
    AGGTTACAGA AGTATGCATA TTCCGTAAGA GATTCAAAAC TGAGAGATAT CTTAAAAGAT
    GGTGCTGCTT ATCATCATGC TGGTATGGAG CTGTCAGATA GAAAAGTAGT TGAGGGAGCT
    TTTACTGTTG GAGATTTACC AGTTCTTTTT ACTACCAGTA CTTTAGCTAT GGGAGTAAAT
    TTGCCTGCTC ACCTAGTAGT TATAAAATCT ACAATGCATT ATGCTGGAGG ACTGTTTGAA
    GAGTACAGTG AAACAGATAT TCTACAGATG ATTGGTAGAG CTGGTCGACC TCAATTTGAC
    ACTACAGCTA CTGCAGTTAT CATGACTCGA TTAAGCACAA GGGACAAGTA CATTCAGATG
    TTAGCTTGTA GAGACACTGT AGAAAGCAGT TTGCACAGAC ATCTTATTGA ACATTTAAAT
    GCAGAGATAG TACTGCATAC CATCACGGAT GTGAATATTG CTGTGGAATG GATACGATCA
    ACTCTGCTTT ATATCAGAGC CTTGAAAAAT CCATCTCATT ATGGTTTTGC ATCTGGATTG
    AACAAAGATG GAATTGAAGC AAAATTACAA GAATTATGTT TGAAGAATCT GAATGATTTA
    TCATCCCTGG ACTTAATAAA GATGGATGAA GGTGTTAATT TCAAACCAAC TGAAGCAGGA
    AGATTGATGG CTTGGTATTA TATTACATTT GAGACAGTGA AGAAATTTTA TACAATCAGT
    GGAAAAGAAA CCTTATCAGA TCTGGTTACA TTGATAGCTG GCTGCAAGGA ATTTCTAGAT
    ATACAGTTAA GGATAAATGA AAAGAAAACA CTGAATACTT TGAACAAAGA TCCAAATCGG
    ATAACTATCA GATTTCCAAT GGAAGGAAGA ATTAAAACAA GAGAAATGAA AGTAAATTGT
    CTTATTCAGG CTCAACTAGG ATGCATTCCC ATACAAGATT TTGCTTTGAC ACAAGATACC
    GCAAAGATTT TCAGACATGG CTCCCGAATT ACAAGATGGT TGTCAGATTT TGTAGCTGCT
    CAAGAAAAGA AGTTTGCTGT ACTATTGAAT AGTTTGATTT TAGCTAAATG TTTTAGGTGT
    AAACTTTGGG AAAACTCTCT GCATGTATCC AAACAACTGG AAAAAATTGG TATAACATTG
    TCAAATGCAA TCGTAAATGC TGGTTTGACT TCCTTTAAAA AAATAGAAGA GACAGATGCA
    AGGGAACTTG AATTGATTTT AAACAGACAT CCCCCCTTTG GAACCCAGAT AAAAGAAACT
    GTGATGTATC TACCAAAATA TGAACTTAAA GTGGAACAGA TTACAAGATA TAGTGATACG
    ACGGCAGAAA TATTAGTGAC TGTTATATTA AGAAATTTTG AACAGCTACA AACTAAAAGA
    ACAGCATCGG ATTCTCACTA TGTTACCTTA ATCATAGGTG ACGCAGATAA TCAAGTAGTT
    TATCTGCACA AGATTACGGA TTCTGTTTTG CTAAAAGCTG GAAGTTGGGC TAAAAAGATT
    GCTGTGAAAA GAGCTCTTAA ATCTGAAGAT CTTAGCATAA ATCTAATAAG TTCTGAATTT
    GTTGGGCTTG ATATTCAGCA GAAACTTACA GTCTTTTACT TAGAACCCAA GAGGTTTGGA
    AATCAAATCA CTATGCAAAG AAAATCTGAA ACACAGATTT CCCATTCTAA ACATTCAGAC
    ATATCTACAA TAGCAGGACC TAATAAAGGA ACGACTGCCA GCAAAAAACC TGGGAACCGA
    GAATGCAATC ATCTTTGTAA AAGTAAACAT ACATGTGGAC ATGACTGCTG TAAAATTGGA
    GTTGCACAGA AGTCAGAAAT TAAAGAGTCA ACAATTTCTT CATATTTATC TGATTTAAGA
    AACAGGAATG CTGTTTCATC TGTTCCTCCA GTTAAACGTC TGAAGATACA GATGAATAAA
    TCTCAAAGTG TGGACCTTAA AGAGTTTGGT TTTACTCCAA AACCTTCCCT TCCTAGTATA
    TCAAGGTCAG AATATTTAAA CATATCTGAA TTGCCTATAA TGGAGCAGTG GGATCAGCCT
    GAAATCTATG GAAAAGTTAG ACAAGAACCA TCTGAATATC AAGACAAAGA AGTTTTGAAT
    GTGAACTTTG AATTGGGAAA TGAAGTTTGG GATGATTTTG ATGATGAAAA CTTAGAAGTT
    ACTAGCTTTT CAACTGATAC TGAGAAGACA AAAATATCAG GATTTGGAAA CACTTTGAGT
    TCAAGTACCA GGGGAAGTAA GCTACCCCTT CAAGAGTCAA AGAGCAAATT CCAAAGAGAA
    ATGTCAAACA GTTTTGTTTC ATCACATGAG ATGTCGGATA TTTCTTTATC AAATTCTGCT
    ATGCCCAAGT TCAGTGCATC CTCCATGACA AAATTACCTC AACAAGCCGG AAATGCAGTT
    ATTGTCCATT TTCAAGAAAG AAAACCACAA AATCTGTCAC CAGAGATTGA GAAGCAATGC
    TTTACTTTCT CTGAAAAAAA CCCAAATTCT TCAAATTATA AAAAAGTGGA TTTTTTTATT
    AGAAACAGTG AATGTAAAAA GGAAGTTGAT TTCAGTATGT ATCATCCTGA TGATGAAGCT
    GATGAAATGA AGTCTTTATT GGGAATATTT GATGGTATTT TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011539159.1
    CDS87..3971
    Translation

    Target ORF information:

    RefSeq Version XM_011540857.1
    Organism Homo sapiens(human)
    Definition Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011540857.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA AATTATTTAA AATATCTGAC AATATACATG GGAGTGCTTA TTCTAATGAC
    AATGAATTGG ACTCTCACAT TGGCTCAGTG AAAATTGTAC AAACAGAAAT GAACAAAGGG
    AAATCAAGGA ACTATAGCAA TAGTAAGCAA AAATTTCAGT ATTCTGCAAA TGTGTTTACA
    GCAAATAATG CTTTTTCTGC TTCTGAAATC GGAGAAGGCA TGTTCAAAGC ACCATCTTTT
    TCAGTTGCTT TCCAACCTCA TGATATTCAA GAGGTAACAG AAAATGGTTT AGGTTCCTTG
    AAGGCTGTCA CAGAAATTCC GGCAAAATTT AGAAGTATTT TCAAAGAATT TCCATATTTC
    AACTATATAC AGTCCAAGGC CTTTGATGAT CTTCTTTACA CAGATAGGAA TTTTGTGATT
    TGTGCTCCAA CTGGTTCTGG AAAAACTGTA GTGTTTGAAC TAGCTATAAC AAGATTGTTA
    ATGGAAGTAC CATTGCCATG GTTGAATATT AAAATTGTTT ACATGGCACC AATAAAAGCC
    TTGTGCAGTC AGCGTTTTGA TGACTGGAAA GAAAAATTTG GACCAATAGG ATTGAATTGT
    AAAGAACTTA CTGGAGATAC AGTAATGGAT GATCTATTTG AGATTCAGCA TGCCCATATT
    ATTATGACAA CTCCAGAAAA ATGGGATAGC ATGACTAGGA AATGGAGAGA CAACTCTTTG
    GTTCAGCTGG TTCGACTGTT TCTCATTGAT GAGGTACATA TTGTAAAAGA TGAAAATCGT
    GGTCCAACTC TTGAAGTTGT AGTTAGCAGA ATGAAAACTG TACAGTCTGT TTCTCAGACT
    TTAAAAAATA CCAGCACTGC TATTCCAATG CGATTTGTAG CTGTATCTGC AACAATTCCA
    AATGCTGAGG ATATTGCAGA ATGGCTTTCA GATGGTGAAA GACCAGCTGT GTGTCTGAAA
    ATGGATGAGA GCCATAGACC AGTGAAACTT CAGAAAGTGG TCCTTGGATT TCCCTGCAGT
    AGTAACCAAA CTGAGTTTAA GTTTGATTTA ACCCTCAACT ACAAAATTGC CAGTGTTATA
    CAAATGTACT CTGATCAGAA ACCCACACTT GTGTTTTGTG CAACAAGGAA GGGTGTGCAA
    CAGGCTGCTT CTGTTCTTGT GAAAGATGCT AAATTTATTA TGACTGTGGA ACAGAAACAG
    AGGTTACAGA AGTATGCATA TTCCGTAAGA GATTCAAAAC TGAGAGATAT CTTAAAAGAT
    GGTGCTGCTT ATCATCATGC TGGTATGGAG CTGTCAGATA GAAAAGTAGT TGAGGGAGCT
    TTTACTGTTG GAGATTTACC AGTTCTTTTT ACTACCAGTA CTTTAGCTAT GGGAGTAAAT
    TTGCCTGCTC ACCTAGTAGT TATAAAATCT ACAATGCATT ATGCTGGAGG ACTGTTTGAA
    GAGTACAGTG AAACAGATAT TCTACAGATG ATTGGTAGAG CTGGTCGACC TCAATTTGAC
    ACTACAGCTA CTGCAGTTAT CATGACTCGA TTAAGCACAA GGGACAAGTA CATTCAGATG
    TTAGCTTGTA GAGACACTGT AGAAAGCAGT TTGCACAGAC ATCTTATTGA ACATTTAAAT
    GCAGAGATAG TACTGCATAC CATCACGGAT GTGAATATTG CTGTGGAATG GATACGATCA
    ACTCTGCTTT ATATCAGAGC CTTGAAAAAT CCATCTCATT ATGGTTTTGC ATCTGGATTG
    AACAAAGATG GAATTGAAGC AAAATTACAA GAATTATGTT TGAAGAATCT GAATGATTTA
    TCATCCCTGG ACTTAATAAA GATGGATGAA GGTGTTAATT TCAAACCAAC TGAAGCAGGA
    AGATTGATGG CTTGGTATTA TATTACATTT GAGACAGTGA AGAAATTTTA TACAATCAGT
    GGAAAAGAAA CCTTATCAGA TCTGGTTACA TTGATAGCTG GCTGCAAGGA ATTTCTAGAT
    ATACAGTTAA GGATAAATGA AAAGAAAACA CTGAATACTT TGAACAAAGA TCCAAATCGG
    ATAACTATCA GATTTCCAAT GGAAGGAAGA ATTAAAACAA GAGAAATGAA AGTAAATTGT
    CTTATTCAGG CTCAACTAGG ATGCATTCCC ATACAAGATT TTGCTTTGAC ACAAGATACC
    GCAAAGATTT TCAGACATGG CTCCCGAATT ACAAGATGGT TGTCAGATTT TGTAGCTGCT
    CAAGAAAAGA AGTTTGCTGT ACTATTGAAT AGTTTGATTT TAGCTAAATG TTTTAGGTGT
    AAACTTTGGG AAAACTCTCT GCATGTATCC AAACAACTGG AAAAAATTGG TATAACATTG
    TCAAATGCAA TCGTAAATGC TGGTTTGACT TCCTTTAAAA AAATAGAAGA GACAGATGCA
    AGGGAACTTG AATTGATTTT AAACAGACAT CCCCCCTTTG GAACCCAGAT AAAAGAAACT
    GTGATGTATC TACCAAAATA TGAACTTAAA GTGGAACAGA TTACAAGATA TAGTGATACG
    ACGGCAGAAA TATTAGTGAC TGTTATATTA AGAAATTTTG AACAGCTACA AACTAAAAGA
    ACAGCATCGG ATTCTCACTA TGTTACCTTA ATCATAGGTG ACGCAGATAA TCAAGTAGTT
    TATCTGCACA AGATTACGGA TTCTGTTTTG CTAAAAGCTG GAAGTTGGGC TAAAAAGATT
    GCTGTGAAAA GAGCTCTTAA ATCTGAAGAT CTTAGCATAA ATCTAATAAG TTCTGAATTT
    GTTGGGCTTG ATATTCAGCA GAAACTTACA GTCTTTTACT TAGAACCCAA GAGGTTTGGA
    AATCAAATCA CTATGCAAAG AAAATCTGAA ACACAGATTT CCCATTCTAA ACATTCAGAC
    ATATCTACAA TAGCAGGACC TAATAAAGGA ACGACTGCCA GCAAAAAACC TGGGAACCGA
    GAATGCAATC ATCTTTGTAA AAGTAAACAT ACATGTGGAC ATGACTGCTG TAAAATTGGA
    GTTGCACAGA AGTCAGAAAT TAAAGAGTCA ACAATTTCTT CATATTTATC TGATTTAAGA
    AACAGGAATG CTGTTTCATC TGTTCCTCCA GTTAAACGTC TGAAGATACA GATGAATAAA
    TCTCAAAGTG TGGACCTTAA AGAGTTTGGT TTTACTCCAA AACCTTCCCT TCCTAGTATA
    TCAAGGTCAG AATATTTAAA CATATCTGAA TTGCCTATAA TGGAGCAGTG GGATCAGCCT
    GAAATCTATG GAAAAGTTAG ACAAGAACCA TCTGAATATC AAGACAAAGA AGTTTTGAAT
    GTGAACTTTG AATTGGGAAA TGAAGTTTGG GATGATTTTG ATGATGAAAA CTTAGAAGTT
    ACTAGCTTTT CAACTGATAC TGAGAAGACA AAAATATCAG GATTTGGAAA CACTTTGAGT
    TCAAGTACCA GGGGAAGTAA GCTACCCCTT CAAGAGTCAA AGAGCAAATT CCAAAGAGAA
    ATGTCAAACA GTTTTGTTTC ATCACATGAG ATGTCGGATA TTTCTTTATC AAATTCTGCT
    ATGCCCAAGT TCAGTGCATC CTCCATGACA AAATTACCTC AACAAGCCGG AAATGCAGTT
    ATTGTCCATT TTCAAGAAAG AAAACCACAA AATCTGTCAC CAGAGATTGA GAAGCAATGC
    TTTACTTTCT CTGAAAAAAA CCCAAATTCT TCAAATTATA AAAAAGTGGA TTTTTTTATT
    AGAAACAGTG AATGTAAAAA GGAAGTTGAT TTCAGTATGT ATCATCCTGA TGATGAAGCT
    GATGAAATGA AGTCTTTATT GGGAATATTT GATGGTATTT TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu77232
    Clone ID Related Accession (Same CDS sequence) XM_017000490.1
    Accession Version XM_017000490.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4227bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product probable ATP-dependent DNA helicase HFM1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA ACCATCCAGA CAATGAAAAG TCATTGGATT GGTTTCTCCC TCCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGAG TTAGAGGAAG AATTAGAAAG TCATAAACTG
    TTAGGTCAGG AAAAGAGGCC AAAAATGTTA ACATCAAATT TAAAGATAAC TAATGAAGAT
    ACAAATTATA TTTCACTAAC ACAAAAATTC CAGTTTGCCT TTCCTTCTGA TAAATATGAA
    CAGGATGATC TAAATTTAGA AGGGGTAGGT AATAATGACT TATCACATAT TGCTGGCAAG
    CTGACATATG CTTCTCAGAA ATATAAAAAT CACATTGGCA CTGAGATAGC ACCTGAGAAG
    AGTGTTCCTG ATGATACAAA ATTAGTTAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GTATTCCGGA AAAGATTATT TAAAATATCT GACAATATAC ATGGGAGTGC TTATTCTAAT
    GACAATGAAT TGGACTCTCA CATTGGCTCA GTGAAAATTG TACAAACAGA AATGAACAAA
    GGGAAATCAA GGAACTATAG CAATAGTAAG CAAAAATTTC AGTATTCTGC AAATGTGTTT
    ACAGCAAATA ATGCTTTTTC TGCTTCTGAA ATCGGAGAAG GCATGTTCAA AGCACCATCT
    TTTTCAGTTG CTTTCCAACC TCATGATATT CAAGAGGTAA CAGAAAATGG TTTAGGTTCC
    TTGAAGGCTG TCACAGAAAT TCCGGCAAAA TTTAGAAGTA TTTTCAAAGA ATTTCCATAT
    TTCAACTATA TACAGTCCAA GGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGAAAAACT GTAGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTTGAAT ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCTTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TTACTGGAGA TACAGTAATG GATGATCTAT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTTCAGC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATATTGTAAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TGTTTCTCAG
    ACTTTAAAAA ATACCAGCAC TGCTATTCCA ATGCGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGACCAGC TGTGTGTCTG
    AAAATGGATG AGAGCCATAG ACCAGTGAAA CTTCAGAAAG TGGTCCTTGG ATTTCCCTGC
    AGTAGTAACC AAACTGAGTT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    ATACAAATGT ACTCTGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAGGGTGTG
    CAACAGGCTG CTTCTGTTCT TGTGAAAGAT GCTAAATTTA TTATGACTGT GGAACAGAAA
    CAGAGGTTAC AGAAGTATGC ATATTCCGTA AGAGATTCAA AACTGAGAGA TATCTTAAAA
    GATGGTGCTG CTTATCATCA TGCTGGTATG GAGCTGTCAG ATAGAAAAGT AGTTGAGGGA
    GCTTTTACTG TTGGAGATTT ACCAGTTCTT TTTACTACCA GTACTTTAGC TATGGGAGTA
    AATTTGCCTG CTCACCTAGT AGTTATAAAA TCTACAATGC ATTATGCTGG AGGACTGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CTACTGCAGT TATCATGACT CGATTAAGCA CAAGGGACAA GTACATTCAG
    ATGTTAGCTT GTAGAGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAACATTTA
    AATGCAGAGA TAGTACTGCA TACCATCACG GATGTGAATA TTGCTGTGGA ATGGATACGA
    TCAACTCTGC TTTATATCAG AGCCTTGAAA AATCCATCTC ATTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA AGCAAAATTA CAAGAAGCAG GAAGATTGAT GGCTTGGTAT
    TATATTACAT TTGAGACAGT GAAGAAATTT TATACAATCA GTGGAAAAGA AACCTTATCA
    GATCTGGTTA CATTGATAGC TGGCTGCAAG GAATTTCTAG ATATACAGTT AAGGATAAAT
    GAAAAGAAAA CACTGAATAC TTTGAACAAA GATCCAAATC GGATAACTAT CAGATTTCCA
    ATGGAAGGAA GAATTAAAAC AAGAGAAATG AAAGTAAATT GTCTTATTCA GGCTCAACTA
    GGATGCATTC CCATACAAGA TTTTGCTTTG ACACAAGATA CCGCAAAGAT TTTCAGACAT
    GGCTCCCGAA TTACAAGATG GTTGTCAGAT TTTGTAGCTG CTCAAGAAAA GAAGTTTGCT
    GTACTATTGA ATAGTTTGAT TTTAGCTAAA TGTTTTAGGT GTAAACTTTG GGAAAACTCT
    CTGCATGTAT CCAAACAACT GGAAAAAATT GGTATAACAT TGTCAAATGC AATCGTAAAT
    GCTGGTTTGA CTTCCTTTAA AAAAATAGAA GAGACAGATG CAAGGGAACT TGAATTGATT
    TTAAACAGAC ATCCCCCCTT TGGAACCCAG ATAAAAGAAA CTGTGATGTA TCTACCAAAA
    TATGAACTTA AAGTGGAACA GATTACAAGA TATAGTGATA CGACGGCAGA AATATTAGTG
    ACTGTTATAT TAAGAAATTT TGAACAGCTA CAAACTAAAA GAACAGCATC GGATTCTCAC
    TATGTTACCT TAATCATAGG TGACGCAGAT AATCAAGTAG TTTATCTGCA CAAGATTACG
    GATTCTGTTT TGCTAAAAGC TGGAAGTTGG GCTAAAAAGA TTGCTGTGAA AAGAGCTCTT
    AAATCTGAAG ATCTTAGCAT AAATCTAATA AGTTCTGAAT TTGTTGGGCT TGATATTCAG
    CAGAAACTTA CAGTCTTTTA CTTAGAACCC AAGAGGTTTG GAAATCAAAT CACTATGCAA
    AGAAAATCTG AAACACAGAT TTCCCATTCT AAACATTCAG ACATATCTAC AATAGCAGGA
    CCTAATAAAG GAACGACTGC CAGCAAAAAA CCTGGGAACC GAGAATGCAA TCATCTTTGT
    AAAAGTAAAC ATACATGTGG ACATGACTGC TGTAAAATTG GAGTTGCACA GAAGTCAGAA
    ATTAAAGAGT CAACAATTTC TTCATATTTA TCTGATTTAA GAAACAGGAA TGCTGTTTCA
    TCTGTTCCTC CAGTTAAACG TCTGAAGATA CAGATGAATA AATCTCAAAG TGTGGACCTT
    AAAGAGTTTG GTTTTACTCC AAAACCTTCC CTTCCTAGTA TATCAAGGTC AGAATATTTA
    AACATATCTG AATTGCCTAT AATGGAGCAG TGGGATCAGC CTGAAATCTA TGGAAAAGTT
    AGACAAGAAC CATCTGAATA TCAAGACAAA GAAGTTTTGA ATGTGAACTT TGAATTGGGA
    AATGAAGTTT GGGATGATTT TGATGATGAA AACTTAGAAG TTACTAGCTT TTCAACTGAT
    ACTGAGAAGA CAAAAATATC AGGATTTGGA AACACTTTGA GTTCAAGTAC CAGGGGAAGT
    AAGCTACCCC TTCAAGAGTC AAAGAGCAAA TTCCAAAGAG AAATGTCAAA CAGTTTTGTT
    TCATCACATG AGATGTCGGA TATTTCTTTA TCAAATTCTG CTATGCCCAA GTTCAGTGCA
    TCCTCCATGA CAAAATTACC TCAACAAGCC GGAAATGCAG TTATTGTCCA TTTTCAAGAA
    AGAAAACCAC AAAATCTGTC ACCAGAGATT GAGAAGCAAT GCTTTACTTT CTCTGAAAAA
    AACCCAAATT CTTCAAATTA TAAAAAAGTG GATTTTTTTA TTAGAAACAG TGAATGTAAA
    AAGGAAGTTG ATTTCAGTAT GTATCATCCT GATGATGAAG CTGATGAAAT GAAGTCTTTA
    TTGGGAATAT TTGATGGTAT TTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016855979.1
    CDS44..4270
    Translation

    Target ORF information:

    RefSeq Version XM_017000490.1
    Organism Homo sapiens(human)
    Definition Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017000490.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA ACCATCCAGA CAATGAAAAG TCATTGGATT GGTTTCTCCC TCCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGAG TTAGAGGAAG AATTAGAAAG TCATAAACTG
    TTAGGTCAGG AAAAGAGGCC AAAAATGTTA ACATCAAATT TAAAGATAAC TAATGAAGAT
    ACAAATTATA TTTCACTAAC ACAAAAATTC CAGTTTGCCT TTCCTTCTGA TAAATATGAA
    CAGGATGATC TAAATTTAGA AGGGGTAGGT AATAATGACT TATCACATAT TGCTGGCAAG
    CTGACATATG CTTCTCAGAA ATATAAAAAT CACATTGGCA CTGAGATAGC ACCTGAGAAG
    AGTGTTCCTG ATGATACAAA ATTAGTTAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GTATTCCGGA AAAGATTATT TAAAATATCT GACAATATAC ATGGGAGTGC TTATTCTAAT
    GACAATGAAT TGGACTCTCA CATTGGCTCA GTGAAAATTG TACAAACAGA AATGAACAAA
    GGGAAATCAA GGAACTATAG CAATAGTAAG CAAAAATTTC AGTATTCTGC AAATGTGTTT
    ACAGCAAATA ATGCTTTTTC TGCTTCTGAA ATCGGAGAAG GCATGTTCAA AGCACCATCT
    TTTTCAGTTG CTTTCCAACC TCATGATATT CAAGAGGTAA CAGAAAATGG TTTAGGTTCC
    TTGAAGGCTG TCACAGAAAT TCCGGCAAAA TTTAGAAGTA TTTTCAAAGA ATTTCCATAT
    TTCAACTATA TACAGTCCAA GGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGAAAAACT GTAGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTTGAAT ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCTTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TTACTGGAGA TACAGTAATG GATGATCTAT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTTCAGC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATATTGTAAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TGTTTCTCAG
    ACTTTAAAAA ATACCAGCAC TGCTATTCCA ATGCGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGACCAGC TGTGTGTCTG
    AAAATGGATG AGAGCCATAG ACCAGTGAAA CTTCAGAAAG TGGTCCTTGG ATTTCCCTGC
    AGTAGTAACC AAACTGAGTT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    ATACAAATGT ACTCTGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAGGGTGTG
    CAACAGGCTG CTTCTGTTCT TGTGAAAGAT GCTAAATTTA TTATGACTGT GGAACAGAAA
    CAGAGGTTAC AGAAGTATGC ATATTCCGTA AGAGATTCAA AACTGAGAGA TATCTTAAAA
    GATGGTGCTG CTTATCATCA TGCTGGTATG GAGCTGTCAG ATAGAAAAGT AGTTGAGGGA
    GCTTTTACTG TTGGAGATTT ACCAGTTCTT TTTACTACCA GTACTTTAGC TATGGGAGTA
    AATTTGCCTG CTCACCTAGT AGTTATAAAA TCTACAATGC ATTATGCTGG AGGACTGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CTACTGCAGT TATCATGACT CGATTAAGCA CAAGGGACAA GTACATTCAG
    ATGTTAGCTT GTAGAGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAACATTTA
    AATGCAGAGA TAGTACTGCA TACCATCACG GATGTGAATA TTGCTGTGGA ATGGATACGA
    TCAACTCTGC TTTATATCAG AGCCTTGAAA AATCCATCTC ATTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA AGCAAAATTA CAAGAAGCAG GAAGATTGAT GGCTTGGTAT
    TATATTACAT TTGAGACAGT GAAGAAATTT TATACAATCA GTGGAAAAGA AACCTTATCA
    GATCTGGTTA CATTGATAGC TGGCTGCAAG GAATTTCTAG ATATACAGTT AAGGATAAAT
    GAAAAGAAAA CACTGAATAC TTTGAACAAA GATCCAAATC GGATAACTAT CAGATTTCCA
    ATGGAAGGAA GAATTAAAAC AAGAGAAATG AAAGTAAATT GTCTTATTCA GGCTCAACTA
    GGATGCATTC CCATACAAGA TTTTGCTTTG ACACAAGATA CCGCAAAGAT TTTCAGACAT
    GGCTCCCGAA TTACAAGATG GTTGTCAGAT TTTGTAGCTG CTCAAGAAAA GAAGTTTGCT
    GTACTATTGA ATAGTTTGAT TTTAGCTAAA TGTTTTAGGT GTAAACTTTG GGAAAACTCT
    CTGCATGTAT CCAAACAACT GGAAAAAATT GGTATAACAT TGTCAAATGC AATCGTAAAT
    GCTGGTTTGA CTTCCTTTAA AAAAATAGAA GAGACAGATG CAAGGGAACT TGAATTGATT
    TTAAACAGAC ATCCCCCCTT TGGAACCCAG ATAAAAGAAA CTGTGATGTA TCTACCAAAA
    TATGAACTTA AAGTGGAACA GATTACAAGA TATAGTGATA CGACGGCAGA AATATTAGTG
    ACTGTTATAT TAAGAAATTT TGAACAGCTA CAAACTAAAA GAACAGCATC GGATTCTCAC
    TATGTTACCT TAATCATAGG TGACGCAGAT AATCAAGTAG TTTATCTGCA CAAGATTACG
    GATTCTGTTT TGCTAAAAGC TGGAAGTTGG GCTAAAAAGA TTGCTGTGAA AAGAGCTCTT
    AAATCTGAAG ATCTTAGCAT AAATCTAATA AGTTCTGAAT TTGTTGGGCT TGATATTCAG
    CAGAAACTTA CAGTCTTTTA CTTAGAACCC AAGAGGTTTG GAAATCAAAT CACTATGCAA
    AGAAAATCTG AAACACAGAT TTCCCATTCT AAACATTCAG ACATATCTAC AATAGCAGGA
    CCTAATAAAG GAACGACTGC CAGCAAAAAA CCTGGGAACC GAGAATGCAA TCATCTTTGT
    AAAAGTAAAC ATACATGTGG ACATGACTGC TGTAAAATTG GAGTTGCACA GAAGTCAGAA
    ATTAAAGAGT CAACAATTTC TTCATATTTA TCTGATTTAA GAAACAGGAA TGCTGTTTCA
    TCTGTTCCTC CAGTTAAACG TCTGAAGATA CAGATGAATA AATCTCAAAG TGTGGACCTT
    AAAGAGTTTG GTTTTACTCC AAAACCTTCC CTTCCTAGTA TATCAAGGTC AGAATATTTA
    AACATATCTG AATTGCCTAT AATGGAGCAG TGGGATCAGC CTGAAATCTA TGGAAAAGTT
    AGACAAGAAC CATCTGAATA TCAAGACAAA GAAGTTTTGA ATGTGAACTT TGAATTGGGA
    AATGAAGTTT GGGATGATTT TGATGATGAA AACTTAGAAG TTACTAGCTT TTCAACTGAT
    ACTGAGAAGA CAAAAATATC AGGATTTGGA AACACTTTGA GTTCAAGTAC CAGGGGAAGT
    AAGCTACCCC TTCAAGAGTC AAAGAGCAAA TTCCAAAGAG AAATGTCAAA CAGTTTTGTT
    TCATCACATG AGATGTCGGA TATTTCTTTA TCAAATTCTG CTATGCCCAA GTTCAGTGCA
    TCCTCCATGA CAAAATTACC TCAACAAGCC GGAAATGCAG TTATTGTCCA TTTTCAAGAA
    AGAAAACCAC AAAATCTGTC ACCAGAGATT GAGAAGCAAT GCTTTACTTT CTCTGAAAAA
    AACCCAAATT CTTCAAATTA TAAAAAAGTG GATTTTTTTA TTAGAAACAG TGAATGTAAA
    AAGGAAGTTG ATTTCAGTAT GTATCATCCT GATGATGAAG CTGATGAAAT GAAGTCTTTA
    TTGGGAATAT TTGATGGTAT TTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu77234
    Clone ID Related Accession (Same CDS sequence) XM_017000491.1
    Accession Version XM_017000491.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4176bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product probable ATP-dependent DNA helicase HFM1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA ACCATCCAGA CAATGAAAAG TCATTGGATT GGTTTCTCCC TCCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGAG TTAGAGGAAG AATTAGAAAG TCATAAACTG
    TTAGGTCAGG AAAAGAGGCC AAAAATGTTA ACATCAAATT TAAAGATAAC TAATGAAGAT
    ACAAATTATA TTTCACTAAC ACAAAAATTC CAGTTTGCCT TTCCTTCTGA TAAATATGAA
    CAGGATGATC TAAATTTAGA AGGGGTAGGT AATAATGACT TATCACATAT TGCTGGCAAG
    CTGACATATG CTTCTCAGAA ATATAAAAAT CACATTGGCA CTGAGATAGC ACCTGAGAAG
    AGTGTTCCTG ATGATACAAA ATTAGTTAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GTATTCCGGA AAAGATTATT TAAAATATCT GACAATATAC ATGGGAGTGC TTATTCTAAT
    GACAATGAAT TGGACTCTCA CATTGGCTCA GTGAAAATTG TACAAACAGA AATGAACAAA
    GGGAAATCAA GGAACTATAG CAATAGTAAG CAAAAATTTC AGTATTCTGC AAATGTGTTT
    ACAGCAAATA ATGCTTTTTC TGCTTCTGAA ATCGGAGAAG GCATGTTCAA AGCACCATCT
    TTTTCAGTTG CTTTCCAACC TCATGATATT CAAGAGGTAA CAGAAAATGG TTTAGGTTCC
    TTGAAGGCTG TCACAGAAAT TCCGGCAAAA TTTAGAAGTA TTTTCAAAGA ATTTCCATAT
    TTCAACTATA TACAGTCCAA GGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGAAAAACT GTAGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTTGAAT ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCTTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TTACTGGAGA TACAGTAATG GATGATCTAT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTTCAGC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATATTGTAAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TGTTTCTCAG
    ACTTTAAAAA ATACCAGCAC TGCTATTCCA ATGCGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGACCAGC TGTGTGTCTG
    AAAATGGATG AGAGCCATAG ACCAGTGAAA CTTCAGAAAG TGGTCCTTGG ATTTCCCTGC
    AGTAGTAACC AAACTGAGTT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    ATACAAATGT ACTCTGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAGGGTGTG
    CAACAGGCTG CTTCTGTTCT TGTGAAAGAT GCTAAATTTA TTATGACTGT GGAACAGAAA
    CAGAGGTTAC AGAAGTATGC ATATTCCGTA AGAGATTCAA AACTGAGAGA TATCTTAAAA
    GATGGTGCTG CTTATCATCA TGCTGGTATG GAGCTGTCAG ATAGAAAAGT AGTTGAGGGA
    GCTTTTACTG TTGGAGATTT ACCAGTTCTT TTTACTACCA GTACTTTAGC TATGGGAGTA
    AATTTGCCTG CTCACCTAGT AGTTATAAAA TCTACAATGC ATTATGCTGG AGGACTGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CTACTGCAGT TATCATGACT CGATTAAGCA CAAGGGACAA GTACATTCAG
    ATGTTAGCTT GTAGAGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAACATTTA
    AATGCAGAGA TAGTACTGCA TACCATCACG GATGTGAATA TTGCTGTGGA ATGGATACGA
    TCAACTCTGC TTTATATCAG AGCCTTGAAA AATCCATCTC ATTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA AGCAAAATTA CAAGAATTAT GTTTGAAGAA TCTGAATGAT
    TTATCATCCC TGGACTTAAT AAAGATGGAT GAAGGTGTTA ATTTCAAACC AACTGAAGCA
    GGAAGATTGA TGGCTTGGTA TTATATTACA TTTGAGACAG TGAAGAAATT TTATACAATC
    AGTGGAAAAG AAACCTTATC AGATCTGGTT ACATTGATAG CTGGCTGCAA GGAATTTCTA
    GATATACAGT TAAGGATAAA TGAAAAGAAA ACACTGAATA CTTTGAACAA AGATCCAAAT
    CGGATAACTA TCAGATTTCC AATGGAAGGA AGAATTAAAA CAAGAGAAAT GAAAGTAAAT
    TGTCTTATTC AGGCTCAACT AGGATGCATT CCCATACAAG ATTTTGCTTT GACACAAGAT
    ACCGCAAAGA TTTTCAGACA TGGCTCCCGA ATTACAAGAT GTATAACATT GTCAAATGCA
    ATCGTAAATG CTGGTTTGAC TTCCTTTAAA AAAATAGAAG AGACAGATGC AAGGGAACTT
    GAATTGATTT TAAACAGACA TCCCCCCTTT GGAACCCAGA TAAAAGAAAC TGTGATGTAT
    CTACCAAAAT ATGAACTTAA AGTGGAACAG ATTACAAGAT ATAGTGATAC GACGGCAGAA
    ATATTAGTGA CTGTTATATT AAGAAATTTT GAACAGCTAC AAACTAAAAG AACAGCATCG
    GATTCTCACT ATGTTACCTT AATCATAGGT GACGCAGATA ATCAAGTAGT TTATCTGCAC
    AAGATTACGG ATTCTGTTTT GCTAAAAGCT GGAAGTTGGG CTAAAAAGAT TGCTGTGAAA
    AGAGCTCTTA AATCTGAAGA TCTTAGCATA AATCTAATAA GTTCTGAATT TGTTGGGCTT
    GATATTCAGC AGAAACTTAC AGTCTTTTAC TTAGAACCCA AGAGGTTTGG AAATCAAATC
    ACTATGCAAA GAAAATCTGA AACACAGATT TCCCATTCTA AACATTCAGA CATATCTACA
    ATAGCAGGAC CTAATAAAGG AACGACTGCC AGCAAAAAAC CTGGGAACCG AGAATGCAAT
    CATCTTTGTA AAAGTAAACA TACATGTGGA CATGACTGCT GTAAAATTGG AGTTGCACAG
    AAGTCAGAAA TTAAAGAGTC AACAATTTCT TCATATTTAT CTGATTTAAG AAACAGGAAT
    GCTGTTTCAT CTGTTCCTCC AGTTAAACGT CTGAAGATAC AGATGAATAA ATCTCAAAGT
    GTGGACCTTA AAGAGTTTGG TTTTACTCCA AAACCTTCCC TTCCTAGTAT ATCAAGGTCA
    GAATATTTAA ACATATCTGA ATTGCCTATA ATGGAGCAGT GGGATCAGCC TGAAATCTAT
    GGAAAAGTTA GACAAGAACC ATCTGAATAT CAAGACAAAG AAGTTTTGAA TGTGAACTTT
    GAATTGGGAA ATGAAGTTTG GGATGATTTT GATGATGAAA ACTTAGAAGT TACTAGCTTT
    TCAACTGATA CTGAGAAGAC AAAAATATCA GGATTTGGAA ACACTTTGAG TTCAAGTACC
    AGGGGAAGTA AGCTACCCCT TCAAGAGTCA AAGAGCAAAT TCCAAAGAGA AATGTCAAAC
    AGTTTTGTTT CATCACATGA GATGTCGGAT ATTTCTTTAT CAAATTCTGC TATGCCCAAG
    TTCAGTGCAT CCTCCATGAC AAAATTACCT CAACAAGCCG GAAATGCAGT TATTGTCCAT
    TTTCAAGAAA GAAAACCACA AAATCTGTCA CCAGAGATTG AGAAGCAATG CTTTACTTTC
    TCTGAAAAAA ACCCAAATTC TTCAAATTAT AAAAAAGTGG ATTTTTTTAT TAGAAACAGT
    GAATGTAAAA AGGAAGTTGA TTTCAGTATG TATCATCCTG ATGATGAAGC TGATGAAATG
    AAGTCTTTAT TGGGAATATT TGATGGTATT TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016855980.1
    CDS44..4219
    Translation

    Target ORF information:

    RefSeq Version XM_017000491.1
    Organism Homo sapiens(human)
    Definition Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017000491.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCTGAAAT CAAATGATTG CCTGTTTTCT TTGGAAAATT TGTTTTTTGA AAAACCAGAT 
    GAAGTTGAAA ACCATCCAGA CAATGAAAAG TCATTGGATT GGTTTCTCCC TCCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGAG TTAGAGGAAG AATTAGAAAG TCATAAACTG
    TTAGGTCAGG AAAAGAGGCC AAAAATGTTA ACATCAAATT TAAAGATAAC TAATGAAGAT
    ACAAATTATA TTTCACTAAC ACAAAAATTC CAGTTTGCCT TTCCTTCTGA TAAATATGAA
    CAGGATGATC TAAATTTAGA AGGGGTAGGT AATAATGACT TATCACATAT TGCTGGCAAG
    CTGACATATG CTTCTCAGAA ATATAAAAAT CACATTGGCA CTGAGATAGC ACCTGAGAAG
    AGTGTTCCTG ATGATACAAA ATTAGTTAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GTATTCCGGA AAAGATTATT TAAAATATCT GACAATATAC ATGGGAGTGC TTATTCTAAT
    GACAATGAAT TGGACTCTCA CATTGGCTCA GTGAAAATTG TACAAACAGA AATGAACAAA
    GGGAAATCAA GGAACTATAG CAATAGTAAG CAAAAATTTC AGTATTCTGC AAATGTGTTT
    ACAGCAAATA ATGCTTTTTC TGCTTCTGAA ATCGGAGAAG GCATGTTCAA AGCACCATCT
    TTTTCAGTTG CTTTCCAACC TCATGATATT CAAGAGGTAA CAGAAAATGG TTTAGGTTCC
    TTGAAGGCTG TCACAGAAAT TCCGGCAAAA TTTAGAAGTA TTTTCAAAGA ATTTCCATAT
    TTCAACTATA TACAGTCCAA GGCCTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGAAAAACT GTAGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTTGAAT ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCTTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TTACTGGAGA TACAGTAATG GATGATCTAT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTTCAGC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATATTGTAAA AGATGAAAAT
    CGTGGTCCAA CTCTTGAAGT TGTAGTTAGC AGAATGAAAA CTGTACAGTC TGTTTCTCAG
    ACTTTAAAAA ATACCAGCAC TGCTATTCCA ATGCGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGACCAGC TGTGTGTCTG
    AAAATGGATG AGAGCCATAG ACCAGTGAAA CTTCAGAAAG TGGTCCTTGG ATTTCCCTGC
    AGTAGTAACC AAACTGAGTT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    ATACAAATGT ACTCTGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAGGGTGTG
    CAACAGGCTG CTTCTGTTCT TGTGAAAGAT GCTAAATTTA TTATGACTGT GGAACAGAAA
    CAGAGGTTAC AGAAGTATGC ATATTCCGTA AGAGATTCAA AACTGAGAGA TATCTTAAAA
    GATGGTGCTG CTTATCATCA TGCTGGTATG GAGCTGTCAG ATAGAAAAGT AGTTGAGGGA
    GCTTTTACTG TTGGAGATTT ACCAGTTCTT TTTACTACCA GTACTTTAGC TATGGGAGTA
    AATTTGCCTG CTCACCTAGT AGTTATAAAA TCTACAATGC ATTATGCTGG AGGACTGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CTACTGCAGT TATCATGACT CGATTAAGCA CAAGGGACAA GTACATTCAG
    ATGTTAGCTT GTAGAGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAACATTTA
    AATGCAGAGA TAGTACTGCA TACCATCACG GATGTGAATA TTGCTGTGGA ATGGATACGA
    TCAACTCTGC TTTATATCAG AGCCTTGAAA AATCCATCTC ATTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA AGCAAAATTA CAAGAATTAT GTTTGAAGAA TCTGAATGAT
    TTATCATCCC TGGACTTAAT AAAGATGGAT GAAGGTGTTA ATTTCAAACC AACTGAAGCA
    GGAAGATTGA TGGCTTGGTA TTATATTACA TTTGAGACAG TGAAGAAATT TTATACAATC
    AGTGGAAAAG AAACCTTATC AGATCTGGTT ACATTGATAG CTGGCTGCAA GGAATTTCTA
    GATATACAGT TAAGGATAAA TGAAAAGAAA ACACTGAATA CTTTGAACAA AGATCCAAAT
    CGGATAACTA TCAGATTTCC AATGGAAGGA AGAATTAAAA CAAGAGAAAT GAAAGTAAAT
    TGTCTTATTC AGGCTCAACT AGGATGCATT CCCATACAAG ATTTTGCTTT GACACAAGAT
    ACCGCAAAGA TTTTCAGACA TGGCTCCCGA ATTACAAGAT GTATAACATT GTCAAATGCA
    ATCGTAAATG CTGGTTTGAC TTCCTTTAAA AAAATAGAAG AGACAGATGC AAGGGAACTT
    GAATTGATTT TAAACAGACA TCCCCCCTTT GGAACCCAGA TAAAAGAAAC TGTGATGTAT
    CTACCAAAAT ATGAACTTAA AGTGGAACAG ATTACAAGAT ATAGTGATAC GACGGCAGAA
    ATATTAGTGA CTGTTATATT AAGAAATTTT GAACAGCTAC AAACTAAAAG AACAGCATCG
    GATTCTCACT ATGTTACCTT AATCATAGGT GACGCAGATA ATCAAGTAGT TTATCTGCAC
    AAGATTACGG ATTCTGTTTT GCTAAAAGCT GGAAGTTGGG CTAAAAAGAT TGCTGTGAAA
    AGAGCTCTTA AATCTGAAGA TCTTAGCATA AATCTAATAA GTTCTGAATT TGTTGGGCTT
    GATATTCAGC AGAAACTTAC AGTCTTTTAC TTAGAACCCA AGAGGTTTGG AAATCAAATC
    ACTATGCAAA GAAAATCTGA AACACAGATT TCCCATTCTA AACATTCAGA CATATCTACA
    ATAGCAGGAC CTAATAAAGG AACGACTGCC AGCAAAAAAC CTGGGAACCG AGAATGCAAT
    CATCTTTGTA AAAGTAAACA TACATGTGGA CATGACTGCT GTAAAATTGG AGTTGCACAG
    AAGTCAGAAA TTAAAGAGTC AACAATTTCT TCATATTTAT CTGATTTAAG AAACAGGAAT
    GCTGTTTCAT CTGTTCCTCC AGTTAAACGT CTGAAGATAC AGATGAATAA ATCTCAAAGT
    GTGGACCTTA AAGAGTTTGG TTTTACTCCA AAACCTTCCC TTCCTAGTAT ATCAAGGTCA
    GAATATTTAA ACATATCTGA ATTGCCTATA ATGGAGCAGT GGGATCAGCC TGAAATCTAT
    GGAAAAGTTA GACAAGAACC ATCTGAATAT CAAGACAAAG AAGTTTTGAA TGTGAACTTT
    GAATTGGGAA ATGAAGTTTG GGATGATTTT GATGATGAAA ACTTAGAAGT TACTAGCTTT
    TCAACTGATA CTGAGAAGAC AAAAATATCA GGATTTGGAA ACACTTTGAG TTCAAGTACC
    AGGGGAAGTA AGCTACCCCT TCAAGAGTCA AAGAGCAAAT TCCAAAGAGA AATGTCAAAC
    AGTTTTGTTT CATCACATGA GATGTCGGAT ATTTCTTTAT CAAATTCTGC TATGCCCAAG
    TTCAGTGCAT CCTCCATGAC AAAATTACCT CAACAAGCCG GAAATGCAGT TATTGTCCAT
    TTTCAAGAAA GAAAACCACA AAATCTGTCA CCAGAGATTG AGAAGCAATG CTTTACTTTC
    TCTGAAAAAA ACCCAAATTC TTCAAATTAT AAAAAAGTGG ATTTTTTTAT TAGAAACAGT
    GAATGTAAAA AGGAAGTTGA TTTCAGTATG TATCATCCTG ATGATGAAGC TGATGAAATG
    AAGTCTTTAT TGGGAATATT TGATGGTATT TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu77236
    Clone ID Related Accession (Same CDS sequence) XM_011540855.1 , XM_011540855.2
    Accession Version XM_011540855.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4182bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product probable ATP-dependent DNA helicase HFM1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGATTGCCT GTTTTCTTTG GAAAATTTGT TTTTTGAAAA ACCAGATGAA GTTGAAAAGT 
    CAGGAAAAGA GGCCAAAAAT GTTAACATCA AATTTAAAGA TAACTAATGA AGATACAAAT
    TATATTTCAC TAACACAAAA ATTCCAGTTT GCCTTTCCTT CTGATAAATA TGAACAGGAT
    GATCTAAATT TAGAAGGGGT AGGTAATAAT GACTTATCAC ATATTGCTGG CAAGCTGACA
    TATGCTTCTC AGAAATATAA AAATCACATT GGCACTGAGA TAGCACCTGA GAAGAGTGTT
    CCTGATGATA CAAAATTAGT TAATTTTGCA GAAGATAAAG GAGAGAGCAC ATCAGTATTC
    CGGAAAAGAT TATTTAAAAT ATCTGACAAT ATACATGGGA GTGCTTATTC TAATGACAAT
    GAATTGGACT CTCACATTGG CTCAGTGAAA ATTGTACAAA CAGAAATGAA CAAAGGGAAA
    TCAAGGAACT ATAGCAATAG TAAGCAAAAA TTTCAGTATT CTGCAAATGT GTTTACAGCA
    AATAATGCTT TTTCTGCTTC TGAAATCGGA GAAGGCATGT TCAAAGCACC ATCTTTTTCA
    GTTGCTTTCC AACCTCATGA TATTCAAGAG GTAACAGAAA ATGGTTTAGG TTCCTTGAAG
    GCTGTCACAG AAATTCCGGC AAAATTTAGA AGTATTTTCA AAGAATTTCC ATATTTCAAC
    TATATACAGT CCAAGGCCTT TGATGATCTT CTTTACACAG ATAGGAATTT TGTGATTTGT
    GCTCCAACTG GTTCTGGAAA AACTGTAGTG TTTGAACTAG CTATAACAAG ATTGTTAATG
    GAAGTACCAT TGCCATGGTT GAATATTAAA ATTGTTTACA TGGCACCAAT AAAAGCCTTG
    TGCAGTCAGC GTTTTGATGA CTGGAAAGAA AAATTTGGAC CAATAGGATT GAATTGTAAA
    GAACTTACTG GAGATACAGT AATGGATGAT CTATTTGAGA TTCAGCATGC CCATATTATT
    ATGACAACTC CAGAAAAATG GGATAGCATG ACTAGGAAAT GGAGAGACAA CTCTTTGGTT
    CAGCTGGTTC GACTGTTTCT CATTGATGAG GTACATATTG TAAAAGATGA AAATCGTGGT
    CCAACTCTTG AAGTTGTAGT TAGCAGAATG AAAACTGTAC AGTCTGTTTC TCAGACTTTA
    AAAAATACCA GCACTGCTAT TCCAATGCGA TTTGTAGCTG TATCTGCAAC AATTCCAAAT
    GCTGAGGATA TTGCAGAATG GCTTTCAGAT GGTGAAAGAC CAGCTGTGTG TCTGAAAATG
    GATGAGAGCC ATAGACCAGT GAAACTTCAG AAAGTGGTCC TTGGATTTCC CTGCAGTAGT
    AACCAAACTG AGTTTAAGTT TGATTTAACC CTCAACTACA AAATTGCCAG TGTTATACAA
    ATGTACTCTG ATCAGAAACC CACACTTGTG TTTTGTGCAA CAAGGAAGGG TGTGCAACAG
    GCTGCTTCTG TTCTTGTGAA AGATGCTAAA TTTATTATGA CTGTGGAACA GAAACAGAGG
    TTACAGAAGT ATGCATATTC CGTAAGAGAT TCAAAACTGA GAGATATCTT AAAAGATGGT
    GCTGCTTATC ATCATGCTGG TATGGAGCTG TCAGATAGAA AAGTAGTTGA GGGAGCTTTT
    ACTGTTGGAG ATTTACCAGT TCTTTTTACT ACCAGTACTT TAGCTATGGG AGTAAATTTG
    CCTGCTCACC TAGTAGTTAT AAAATCTACA ATGCATTATG CTGGAGGACT GTTTGAAGAG
    TACAGTGAAA CAGATATTCT ACAGATGATT GGTAGAGCTG GTCGACCTCA ATTTGACACT
    ACAGCTACTG CAGTTATCAT GACTCGATTA AGCACAAGGG ACAAGTACAT TCAGATGTTA
    GCTTGTAGAG ACACTGTAGA AAGCAGTTTG CACAGACATC TTATTGAACA TTTAAATGCA
    GAGATAGTAC TGCATACCAT CACGGATGTG AATATTGCTG TGGAATGGAT ACGATCAACT
    CTGCTTTATA TCAGAGCCTT GAAAAATCCA TCTCATTATG GTTTTGCATC TGGATTGAAC
    AAAGATGGAA TTGAAGCAAA ATTACAAGAA TTATGTTTGA AGAATCTGAA TGATTTATCA
    TCCCTGGACT TAATAAAGAT GGATGAAGGT GTTAATTTCA AACCAACTGA AGCAGGAAGA
    TTGATGGCTT GGTATTATAT TACATTTGAG ACAGTGAAGA AATTTTATAC AATCAGTGGA
    AAAGAAACCT TATCAGATCT GGTTACATTG ATAGCTGGCT GCAAGGAATT TCTAGATATA
    CAGTTAAGGA TAAATGAAAA GAAAACACTG AATACTTTGA ACAAAGATCC AAATCGGATA
    ACTATCAGAT TTCCAATGGA AGGAAGAATT AAAACAAGAG AAATGAAAGT AAATTGTCTT
    ATTCAGGCTC AACTAGGATG CATTCCCATA CAAGATTTTG CTTTGACACA AGATACCGCA
    AAGATTTTCA GACATGGCTC CCGAATTACA AGATGGTTGT CAGATTTTGT AGCTGCTCAA
    GAAAAGAAGT TTGCTGTACT ATTGAATAGT TTGATTTTAG CTAAATGTTT TAGGTGTAAA
    CTTTGGGAAA ACTCTCTGCA TGTATCCAAA CAACTGGAAA AAATTGGTAT AACATTGTCA
    AATGCAATCG TAAATGCTGG TTTGACTTCC TTTAAAAAAA TAGAAGAGAC AGATGCAAGG
    GAACTTGAAT TGATTTTAAA CAGACATCCC CCCTTTGGAA CCCAGATAAA AGAAACTGTG
    ATGTATCTAC CAAAATATGA ACTTAAAGTG GAACAGATTA CAAGATATAG TGATACGACG
    GCAGAAATAT TAGTGACTGT TATATTAAGA AATTTTGAAC AGCTACAAAC TAAAAGAACA
    GCATCGGATT CTCACTATGT TACCTTAATC ATAGGTGACG CAGATAATCA AGTAGTTTAT
    CTGCACAAGA TTACGGATTC TGTTTTGCTA AAAGCTGGAA GTTGGGCTAA AAAGATTGCT
    GTGAAAAGAG CTCTTAAATC TGAAGATCTT AGCATAAATC TAATAAGTTC TGAATTTGTT
    GGGCTTGATA TTCAGCAGAA ACTTACAGTC TTTTACTTAG AACCCAAGAG GTTTGGAAAT
    CAAATCACTA TGCAAAGAAA ATCTGAAACA CAGATTTCCC ATTCTAAACA TTCAGACATA
    TCTACAATAG CAGGACCTAA TAAAGGAACG ACTGCCAGCA AAAAACCTGG GAACCGAGAA
    TGCAATCATC TTTGTAAAAG TAAACATACA TGTGGACATG ACTGCTGTAA AATTGGAGTT
    GCACAGAAGT CAGAAATTAA AGAGTCAACA ATTTCTTCAT ATTTATCTGA TTTAAGAAAC
    AGGAATGCTG TTTCATCTGT TCCTCCAGTT AAACGTCTGA AGATACAGAT GAATAAATCT
    CAAAGTGTGG ACCTTAAAGA GTTTGGTTTT ACTCCAAAAC CTTCCCTTCC TAGTATATCA
    AGGTCAGAAT ATTTAAACAT ATCTGAATTG CCTATAATGG AGCAGTGGGA TCAGCCTGAA
    ATCTATGGAA AAGTTAGACA AGAACCATCT GAATATCAAG ACAAAGAAGT TTTGAATGTG
    AACTTTGAAT TGGGAAATGA AGTTTGGGAT GATTTTGATG ATGAAAACTT AGAAGTTACT
    AGCTTTTCAA CTGATACTGA GAAGACAAAA ATATCAGGAT TTGGAAACAC TTTGAGTTCA
    AGTACCAGGG GAAGTAAGCT ACCCCTTCAA GAGTCAAAGA GCAAATTCCA AAGAGAAATG
    TCAAACAGTT TTGTTTCATC ACATGAGATG TCGGATATTT CTTTATCAAA TTCTGCTATG
    CCCAAGTTCA GTGCATCCTC CATGACAAAA TTACCTCAAC AAGCCGGAAA TGCAGTTATT
    GTCCATTTTC AAGAAAGAAA ACCACAAAAT CTGTCACCAG AGATTGAGAA GCAATGCTTT
    ACTTTCTCTG AAAAAAACCC AAATTCTTCA AATTATAAAA AAGTGGATTT TTTTATTAGA
    AACAGTGAAT GTAAAAAGGA AGTTGATTTC AGTATGTATC ATCCTGATGA TGAAGCTGAT
    GAAATGAAGT CTTTATTGGG AATATTTGAT GGTATTTTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011539157.1
    CDS31..4212
    Translation

    Target ORF information:

    RefSeq Version XM_011540855.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011540855.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGATTGCCT GTTTTCTTTG GAAAATTTGT TTTTTGAAAA ACCAGATGAA GTTGAAAAGT 
    CAGGAAAAGA GGCCAAAAAT GTTAACATCA AATTTAAAGA TAACTAATGA AGATACAAAT
    TATATTTCAC TAACACAAAA ATTCCAGTTT GCCTTTCCTT CTGATAAATA TGAACAGGAT
    GATCTAAATT TAGAAGGGGT AGGTAATAAT GACTTATCAC ATATTGCTGG CAAGCTGACA
    TATGCTTCTC AGAAATATAA AAATCACATT GGCACTGAGA TAGCACCTGA GAAGAGTGTT
    CCTGATGATA CAAAATTAGT TAATTTTGCA GAAGATAAAG GAGAGAGCAC ATCAGTATTC
    CGGAAAAGAT TATTTAAAAT ATCTGACAAT ATACATGGGA GTGCTTATTC TAATGACAAT
    GAATTGGACT CTCACATTGG CTCAGTGAAA ATTGTACAAA CAGAAATGAA CAAAGGGAAA
    TCAAGGAACT ATAGCAATAG TAAGCAAAAA TTTCAGTATT CTGCAAATGT GTTTACAGCA
    AATAATGCTT TTTCTGCTTC TGAAATCGGA GAAGGCATGT TCAAAGCACC ATCTTTTTCA
    GTTGCTTTCC AACCTCATGA TATTCAAGAG GTAACAGAAA ATGGTTTAGG TTCCTTGAAG
    GCTGTCACAG AAATTCCGGC AAAATTTAGA AGTATTTTCA AAGAATTTCC ATATTTCAAC
    TATATACAGT CCAAGGCCTT TGATGATCTT CTTTACACAG ATAGGAATTT TGTGATTTGT
    GCTCCAACTG GTTCTGGAAA AACTGTAGTG TTTGAACTAG CTATAACAAG ATTGTTAATG
    GAAGTACCAT TGCCATGGTT GAATATTAAA ATTGTTTACA TGGCACCAAT AAAAGCCTTG
    TGCAGTCAGC GTTTTGATGA CTGGAAAGAA AAATTTGGAC CAATAGGATT GAATTGTAAA
    GAACTTACTG GAGATACAGT AATGGATGAT CTATTTGAGA TTCAGCATGC CCATATTATT
    ATGACAACTC CAGAAAAATG GGATAGCATG ACTAGGAAAT GGAGAGACAA CTCTTTGGTT
    CAGCTGGTTC GACTGTTTCT CATTGATGAG GTACATATTG TAAAAGATGA AAATCGTGGT
    CCAACTCTTG AAGTTGTAGT TAGCAGAATG AAAACTGTAC AGTCTGTTTC TCAGACTTTA
    AAAAATACCA GCACTGCTAT TCCAATGCGA TTTGTAGCTG TATCTGCAAC AATTCCAAAT
    GCTGAGGATA TTGCAGAATG GCTTTCAGAT GGTGAAAGAC CAGCTGTGTG TCTGAAAATG
    GATGAGAGCC ATAGACCAGT GAAACTTCAG AAAGTGGTCC TTGGATTTCC CTGCAGTAGT
    AACCAAACTG AGTTTAAGTT TGATTTAACC CTCAACTACA AAATTGCCAG TGTTATACAA
    ATGTACTCTG ATCAGAAACC CACACTTGTG TTTTGTGCAA CAAGGAAGGG TGTGCAACAG
    GCTGCTTCTG TTCTTGTGAA AGATGCTAAA TTTATTATGA CTGTGGAACA GAAACAGAGG
    TTACAGAAGT ATGCATATTC CGTAAGAGAT TCAAAACTGA GAGATATCTT AAAAGATGGT
    GCTGCTTATC ATCATGCTGG TATGGAGCTG TCAGATAGAA AAGTAGTTGA GGGAGCTTTT
    ACTGTTGGAG ATTTACCAGT TCTTTTTACT ACCAGTACTT TAGCTATGGG AGTAAATTTG
    CCTGCTCACC TAGTAGTTAT AAAATCTACA ATGCATTATG CTGGAGGACT GTTTGAAGAG
    TACAGTGAAA CAGATATTCT ACAGATGATT GGTAGAGCTG GTCGACCTCA ATTTGACACT
    ACAGCTACTG CAGTTATCAT GACTCGATTA AGCACAAGGG ACAAGTACAT TCAGATGTTA
    GCTTGTAGAG ACACTGTAGA AAGCAGTTTG CACAGACATC TTATTGAACA TTTAAATGCA
    GAGATAGTAC TGCATACCAT CACGGATGTG AATATTGCTG TGGAATGGAT ACGATCAACT
    CTGCTTTATA TCAGAGCCTT GAAAAATCCA TCTCATTATG GTTTTGCATC TGGATTGAAC
    AAAGATGGAA TTGAAGCAAA ATTACAAGAA TTATGTTTGA AGAATCTGAA TGATTTATCA
    TCCCTGGACT TAATAAAGAT GGATGAAGGT GTTAATTTCA AACCAACTGA AGCAGGAAGA
    TTGATGGCTT GGTATTATAT TACATTTGAG ACAGTGAAGA AATTTTATAC AATCAGTGGA
    AAAGAAACCT TATCAGATCT GGTTACATTG ATAGCTGGCT GCAAGGAATT TCTAGATATA
    CAGTTAAGGA TAAATGAAAA GAAAACACTG AATACTTTGA ACAAAGATCC AAATCGGATA
    ACTATCAGAT TTCCAATGGA AGGAAGAATT AAAACAAGAG AAATGAAAGT AAATTGTCTT
    ATTCAGGCTC AACTAGGATG CATTCCCATA CAAGATTTTG CTTTGACACA AGATACCGCA
    AAGATTTTCA GACATGGCTC CCGAATTACA AGATGGTTGT CAGATTTTGT AGCTGCTCAA
    GAAAAGAAGT TTGCTGTACT ATTGAATAGT TTGATTTTAG CTAAATGTTT TAGGTGTAAA
    CTTTGGGAAA ACTCTCTGCA TGTATCCAAA CAACTGGAAA AAATTGGTAT AACATTGTCA
    AATGCAATCG TAAATGCTGG TTTGACTTCC TTTAAAAAAA TAGAAGAGAC AGATGCAAGG
    GAACTTGAAT TGATTTTAAA CAGACATCCC CCCTTTGGAA CCCAGATAAA AGAAACTGTG
    ATGTATCTAC CAAAATATGA ACTTAAAGTG GAACAGATTA CAAGATATAG TGATACGACG
    GCAGAAATAT TAGTGACTGT TATATTAAGA AATTTTGAAC AGCTACAAAC TAAAAGAACA
    GCATCGGATT CTCACTATGT TACCTTAATC ATAGGTGACG CAGATAATCA AGTAGTTTAT
    CTGCACAAGA TTACGGATTC TGTTTTGCTA AAAGCTGGAA GTTGGGCTAA AAAGATTGCT
    GTGAAAAGAG CTCTTAAATC TGAAGATCTT AGCATAAATC TAATAAGTTC TGAATTTGTT
    GGGCTTGATA TTCAGCAGAA ACTTACAGTC TTTTACTTAG AACCCAAGAG GTTTGGAAAT
    CAAATCACTA TGCAAAGAAA ATCTGAAACA CAGATTTCCC ATTCTAAACA TTCAGACATA
    TCTACAATAG CAGGACCTAA TAAAGGAACG ACTGCCAGCA AAAAACCTGG GAACCGAGAA
    TGCAATCATC TTTGTAAAAG TAAACATACA TGTGGACATG ACTGCTGTAA AATTGGAGTT
    GCACAGAAGT CAGAAATTAA AGAGTCAACA ATTTCTTCAT ATTTATCTGA TTTAAGAAAC
    AGGAATGCTG TTTCATCTGT TCCTCCAGTT AAACGTCTGA AGATACAGAT GAATAAATCT
    CAAAGTGTGG ACCTTAAAGA GTTTGGTTTT ACTCCAAAAC CTTCCCTTCC TAGTATATCA
    AGGTCAGAAT ATTTAAACAT ATCTGAATTG CCTATAATGG AGCAGTGGGA TCAGCCTGAA
    ATCTATGGAA AAGTTAGACA AGAACCATCT GAATATCAAG ACAAAGAAGT TTTGAATGTG
    AACTTTGAAT TGGGAAATGA AGTTTGGGAT GATTTTGATG ATGAAAACTT AGAAGTTACT
    AGCTTTTCAA CTGATACTGA GAAGACAAAA ATATCAGGAT TTGGAAACAC TTTGAGTTCA
    AGTACCAGGG GAAGTAAGCT ACCCCTTCAA GAGTCAAAGA GCAAATTCCA AAGAGAAATG
    TCAAACAGTT TTGTTTCATC ACATGAGATG TCGGATATTT CTTTATCAAA TTCTGCTATG
    CCCAAGTTCA GTGCATCCTC CATGACAAAA TTACCTCAAC AAGCCGGAAA TGCAGTTATT
    GTCCATTTTC AAGAAAGAAA ACCACAAAAT CTGTCACCAG AGATTGAGAA GCAATGCTTT
    ACTTTCTCTG AAAAAAACCC AAATTCTTCA AATTATAAAA AAGTGGATTT TTTTATTAGA
    AACAGTGAAT GTAAAAAGGA AGTTGATTTC AGTATGTATC ATCCTGATGA TGAAGCTGAT
    GAAATGAAGT CTTTATTGGG AATATTTGAT GGTATTTTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu77236
    Clone ID Related Accession (Same CDS sequence) XM_011540855.1 , XM_011540855.2
    Accession Version XM_011540855.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4182bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product probable ATP-dependent DNA helicase HFM1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011540855.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGATTGCCT GTTTTCTTTG GAAAATTTGT TTTTTGAAAA ACCAGATGAA GTTGAAAAGT 
    CAGGAAAAGA GGCCAAAAAT GTTAACATCA AATTTAAAGA TAACTAATGA AGATACAAAT
    TATATTTCAC TAACACAAAA ATTCCAGTTT GCCTTTCCTT CTGATAAATA TGAACAGGAT
    GATCTAAATT TAGAAGGGGT AGGTAATAAT GACTTATCAC ATATTGCTGG CAAGCTGACA
    TATGCTTCTC AGAAATATAA AAATCACATT GGCACTGAGA TAGCACCTGA GAAGAGTGTT
    CCTGATGATA CAAAATTAGT TAATTTTGCA GAAGATAAAG GAGAGAGCAC ATCAGTATTC
    CGGAAAAGAT TATTTAAAAT ATCTGACAAT ATACATGGGA GTGCTTATTC TAATGACAAT
    GAATTGGACT CTCACATTGG CTCAGTGAAA ATTGTACAAA CAGAAATGAA CAAAGGGAAA
    TCAAGGAACT ATAGCAATAG TAAGCAAAAA TTTCAGTATT CTGCAAATGT GTTTACAGCA
    AATAATGCTT TTTCTGCTTC TGAAATCGGA GAAGGCATGT TCAAAGCACC ATCTTTTTCA
    GTTGCTTTCC AACCTCATGA TATTCAAGAG GTAACAGAAA ATGGTTTAGG TTCCTTGAAG
    GCTGTCACAG AAATTCCGGC AAAATTTAGA AGTATTTTCA AAGAATTTCC ATATTTCAAC
    TATATACAGT CCAAGGCCTT TGATGATCTT CTTTACACAG ATAGGAATTT TGTGATTTGT
    GCTCCAACTG GTTCTGGAAA AACTGTAGTG TTTGAACTAG CTATAACAAG ATTGTTAATG
    GAAGTACCAT TGCCATGGTT GAATATTAAA ATTGTTTACA TGGCACCAAT AAAAGCCTTG
    TGCAGTCAGC GTTTTGATGA CTGGAAAGAA AAATTTGGAC CAATAGGATT GAATTGTAAA
    GAACTTACTG GAGATACAGT AATGGATGAT CTATTTGAGA TTCAGCATGC CCATATTATT
    ATGACAACTC CAGAAAAATG GGATAGCATG ACTAGGAAAT GGAGAGACAA CTCTTTGGTT
    CAGCTGGTTC GACTGTTTCT CATTGATGAG GTACATATTG TAAAAGATGA AAATCGTGGT
    CCAACTCTTG AAGTTGTAGT TAGCAGAATG AAAACTGTAC AGTCTGTTTC TCAGACTTTA
    AAAAATACCA GCACTGCTAT TCCAATGCGA TTTGTAGCTG TATCTGCAAC AATTCCAAAT
    GCTGAGGATA TTGCAGAATG GCTTTCAGAT GGTGAAAGAC CAGCTGTGTG TCTGAAAATG
    GATGAGAGCC ATAGACCAGT GAAACTTCAG AAAGTGGTCC TTGGATTTCC CTGCAGTAGT
    AACCAAACTG AGTTTAAGTT TGATTTAACC CTCAACTACA AAATTGCCAG TGTTATACAA
    ATGTACTCTG ATCAGAAACC CACACTTGTG TTTTGTGCAA CAAGGAAGGG TGTGCAACAG
    GCTGCTTCTG TTCTTGTGAA AGATGCTAAA TTTATTATGA CTGTGGAACA GAAACAGAGG
    TTACAGAAGT ATGCATATTC CGTAAGAGAT TCAAAACTGA GAGATATCTT AAAAGATGGT
    GCTGCTTATC ATCATGCTGG TATGGAGCTG TCAGATAGAA AAGTAGTTGA GGGAGCTTTT
    ACTGTTGGAG ATTTACCAGT TCTTTTTACT ACCAGTACTT TAGCTATGGG AGTAAATTTG
    CCTGCTCACC TAGTAGTTAT AAAATCTACA ATGCATTATG CTGGAGGACT GTTTGAAGAG
    TACAGTGAAA CAGATATTCT ACAGATGATT GGTAGAGCTG GTCGACCTCA ATTTGACACT
    ACAGCTACTG CAGTTATCAT GACTCGATTA AGCACAAGGG ACAAGTACAT TCAGATGTTA
    GCTTGTAGAG ACACTGTAGA AAGCAGTTTG CACAGACATC TTATTGAACA TTTAAATGCA
    GAGATAGTAC TGCATACCAT CACGGATGTG AATATTGCTG TGGAATGGAT ACGATCAACT
    CTGCTTTATA TCAGAGCCTT GAAAAATCCA TCTCATTATG GTTTTGCATC TGGATTGAAC
    AAAGATGGAA TTGAAGCAAA ATTACAAGAA TTATGTTTGA AGAATCTGAA TGATTTATCA
    TCCCTGGACT TAATAAAGAT GGATGAAGGT GTTAATTTCA AACCAACTGA AGCAGGAAGA
    TTGATGGCTT GGTATTATAT TACATTTGAG ACAGTGAAGA AATTTTATAC AATCAGTGGA
    AAAGAAACCT TATCAGATCT GGTTACATTG ATAGCTGGCT GCAAGGAATT TCTAGATATA
    CAGTTAAGGA TAAATGAAAA GAAAACACTG AATACTTTGA ACAAAGATCC AAATCGGATA
    ACTATCAGAT TTCCAATGGA AGGAAGAATT AAAACAAGAG AAATGAAAGT AAATTGTCTT
    ATTCAGGCTC AACTAGGATG CATTCCCATA CAAGATTTTG CTTTGACACA AGATACCGCA
    AAGATTTTCA GACATGGCTC CCGAATTACA AGATGGTTGT CAGATTTTGT AGCTGCTCAA
    GAAAAGAAGT TTGCTGTACT ATTGAATAGT TTGATTTTAG CTAAATGTTT TAGGTGTAAA
    CTTTGGGAAA ACTCTCTGCA TGTATCCAAA CAACTGGAAA AAATTGGTAT AACATTGTCA
    AATGCAATCG TAAATGCTGG TTTGACTTCC TTTAAAAAAA TAGAAGAGAC AGATGCAAGG
    GAACTTGAAT TGATTTTAAA CAGACATCCC CCCTTTGGAA CCCAGATAAA AGAAACTGTG
    ATGTATCTAC CAAAATATGA ACTTAAAGTG GAACAGATTA CAAGATATAG TGATACGACG
    GCAGAAATAT TAGTGACTGT TATATTAAGA AATTTTGAAC AGCTACAAAC TAAAAGAACA
    GCATCGGATT CTCACTATGT TACCTTAATC ATAGGTGACG CAGATAATCA AGTAGTTTAT
    CTGCACAAGA TTACGGATTC TGTTTTGCTA AAAGCTGGAA GTTGGGCTAA AAAGATTGCT
    GTGAAAAGAG CTCTTAAATC TGAAGATCTT AGCATAAATC TAATAAGTTC TGAATTTGTT
    GGGCTTGATA TTCAGCAGAA ACTTACAGTC TTTTACTTAG AACCCAAGAG GTTTGGAAAT
    CAAATCACTA TGCAAAGAAA ATCTGAAACA CAGATTTCCC ATTCTAAACA TTCAGACATA
    TCTACAATAG CAGGACCTAA TAAAGGAACG ACTGCCAGCA AAAAACCTGG GAACCGAGAA
    TGCAATCATC TTTGTAAAAG TAAACATACA TGTGGACATG ACTGCTGTAA AATTGGAGTT
    GCACAGAAGT CAGAAATTAA AGAGTCAACA ATTTCTTCAT ATTTATCTGA TTTAAGAAAC
    AGGAATGCTG TTTCATCTGT TCCTCCAGTT AAACGTCTGA AGATACAGAT GAATAAATCT
    CAAAGTGTGG ACCTTAAAGA GTTTGGTTTT ACTCCAAAAC CTTCCCTTCC TAGTATATCA
    AGGTCAGAAT ATTTAAACAT ATCTGAATTG CCTATAATGG AGCAGTGGGA TCAGCCTGAA
    ATCTATGGAA AAGTTAGACA AGAACCATCT GAATATCAAG ACAAAGAAGT TTTGAATGTG
    AACTTTGAAT TGGGAAATGA AGTTTGGGAT GATTTTGATG ATGAAAACTT AGAAGTTACT
    AGCTTTTCAA CTGATACTGA GAAGACAAAA ATATCAGGAT TTGGAAACAC TTTGAGTTCA
    AGTACCAGGG GAAGTAAGCT ACCCCTTCAA GAGTCAAAGA GCAAATTCCA AAGAGAAATG
    TCAAACAGTT TTGTTTCATC ACATGAGATG TCGGATATTT CTTTATCAAA TTCTGCTATG
    CCCAAGTTCA GTGCATCCTC CATGACAAAA TTACCTCAAC AAGCCGGAAA TGCAGTTATT
    GTCCATTTTC AAGAAAGAAA ACCACAAAAT CTGTCACCAG AGATTGAGAA GCAATGCTTT
    ACTTTCTCTG AAAAAAACCC AAATTCTTCA AATTATAAAA AAGTGGATTT TTTTATTAGA
    AACAGTGAAT GTAAAAAGGA AGTTGATTTC AGTATGTATC ATCCTGATGA TGAAGCTGAT
    GAAATGAAGT CTTTATTGGG AATATTTGAT GGTATTTTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011539157.1
    CDS28..4209
    Translation

    Target ORF information:

    RefSeq Version XM_011540855.2
    Organism Homo sapiens(human)
    Definition Homo sapiens helicase for meiosis 1 (HFM1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011540855.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGATTGCCT GTTTTCTTTG GAAAATTTGT TTTTTGAAAA ACCAGATGAA GTTGAAAAGT 
    CAGGAAAAGA GGCCAAAAAT GTTAACATCA AATTTAAAGA TAACTAATGA AGATACAAAT
    TATATTTCAC TAACACAAAA ATTCCAGTTT GCCTTTCCTT CTGATAAATA TGAACAGGAT
    GATCTAAATT TAGAAGGGGT AGGTAATAAT GACTTATCAC ATATTGCTGG CAAGCTGACA
    TATGCTTCTC AGAAATATAA AAATCACATT GGCACTGAGA TAGCACCTGA GAAGAGTGTT
    CCTGATGATA CAAAATTAGT TAATTTTGCA GAAGATAAAG GAGAGAGCAC ATCAGTATTC
    CGGAAAAGAT TATTTAAAAT ATCTGACAAT ATACATGGGA GTGCTTATTC TAATGACAAT
    GAATTGGACT CTCACATTGG CTCAGTGAAA ATTGTACAAA CAGAAATGAA CAAAGGGAAA
    TCAAGGAACT ATAGCAATAG TAAGCAAAAA TTTCAGTATT CTGCAAATGT GTTTACAGCA
    AATAATGCTT TTTCTGCTTC TGAAATCGGA GAAGGCATGT TCAAAGCACC ATCTTTTTCA
    GTTGCTTTCC AACCTCATGA TATTCAAGAG GTAACAGAAA ATGGTTTAGG TTCCTTGAAG
    GCTGTCACAG AAATTCCGGC AAAATTTAGA AGTATTTTCA AAGAATTTCC ATATTTCAAC
    TATATACAGT CCAAGGCCTT TGATGATCTT CTTTACACAG ATAGGAATTT TGTGATTTGT
    GCTCCAACTG GTTCTGGAAA AACTGTAGTG TTTGAACTAG CTATAACAAG ATTGTTAATG
    GAAGTACCAT TGCCATGGTT GAATATTAAA ATTGTTTACA TGGCACCAAT AAAAGCCTTG
    TGCAGTCAGC GTTTTGATGA CTGGAAAGAA AAATTTGGAC CAATAGGATT GAATTGTAAA
    GAACTTACTG GAGATACAGT AATGGATGAT CTATTTGAGA TTCAGCATGC CCATATTATT
    ATGACAACTC CAGAAAAATG GGATAGCATG ACTAGGAAAT GGAGAGACAA CTCTTTGGTT
    CAGCTGGTTC GACTGTTTCT CATTGATGAG GTACATATTG TAAAAGATGA AAATCGTGGT
    CCAACTCTTG AAGTTGTAGT TAGCAGAATG AAAACTGTAC AGTCTGTTTC TCAGACTTTA
    AAAAATACCA GCACTGCTAT TCCAATGCGA TTTGTAGCTG TATCTGCAAC AATTCCAAAT
    GCTGAGGATA TTGCAGAATG GCTTTCAGAT GGTGAAAGAC CAGCTGTGTG TCTGAAAATG
    GATGAGAGCC ATAGACCAGT GAAACTTCAG AAAGTGGTCC TTGGATTTCC CTGCAGTAGT
    AACCAAACTG AGTTTAAGTT TGATTTAACC CTCAACTACA AAATTGCCAG TGTTATACAA
    ATGTACTCTG ATCAGAAACC CACACTTGTG TTTTGTGCAA CAAGGAAGGG TGTGCAACAG
    GCTGCTTCTG TTCTTGTGAA AGATGCTAAA TTTATTATGA CTGTGGAACA GAAACAGAGG
    TTACAGAAGT ATGCATATTC CGTAAGAGAT TCAAAACTGA GAGATATCTT AAAAGATGGT
    GCTGCTTATC ATCATGCTGG TATGGAGCTG TCAGATAGAA AAGTAGTTGA GGGAGCTTTT
    ACTGTTGGAG ATTTACCAGT TCTTTTTACT ACCAGTACTT TAGCTATGGG AGTAAATTTG
    CCTGCTCACC TAGTAGTTAT AAAATCTACA ATGCATTATG CTGGAGGACT GTTTGAAGAG
    TACAGTGAAA CAGATATTCT ACAGATGATT GGTAGAGCTG GTCGACCTCA ATTTGACACT
    ACAGCTACTG CAGTTATCAT GACTCGATTA AGCACAAGGG ACAAGTACAT TCAGATGTTA
    GCTTGTAGAG ACACTGTAGA AAGCAGTTTG CACAGACATC TTATTGAACA TTTAAATGCA
    GAGATAGTAC TGCATACCAT CACGGATGTG AATATTGCTG TGGAATGGAT ACGATCAACT
    CTGCTTTATA TCAGAGCCTT GAAAAATCCA TCTCATTATG GTTTTGCATC TGGATTGAAC
    AAAGATGGAA TTGAAGCAAA ATTACAAGAA TTATGTTTGA AGAATCTGAA TGATTTATCA
    TCCCTGGACT TAATAAAGAT GGATGAAGGT GTTAATTTCA AACCAACTGA AGCAGGAAGA
    TTGATGGCTT GGTATTATAT TACATTTGAG ACAGTGAAGA AATTTTATAC AATCAGTGGA
    AAAGAAACCT TATCAGATCT GGTTACATTG ATAGCTGGCT GCAAGGAATT TCTAGATATA
    CAGTTAAGGA TAAATGAAAA GAAAACACTG AATACTTTGA ACAAAGATCC AAATCGGATA
    ACTATCAGAT TTCCAATGGA AGGAAGAATT AAAACAAGAG AAATGAAAGT AAATTGTCTT
    ATTCAGGCTC AACTAGGATG CATTCCCATA CAAGATTTTG CTTTGACACA AGATACCGCA
    AAGATTTTCA GACATGGCTC CCGAATTACA AGATGGTTGT CAGATTTTGT AGCTGCTCAA
    GAAAAGAAGT TTGCTGTACT ATTGAATAGT TTGATTTTAG CTAAATGTTT TAGGTGTAAA
    CTTTGGGAAA ACTCTCTGCA TGTATCCAAA CAACTGGAAA AAATTGGTAT AACATTGTCA
    AATGCAATCG TAAATGCTGG TTTGACTTCC TTTAAAAAAA TAGAAGAGAC AGATGCAAGG
    GAACTTGAAT TGATTTTAAA CAGACATCCC CCCTTTGGAA CCCAGATAAA AGAAACTGTG
    ATGTATCTAC CAAAATATGA ACTTAAAGTG GAACAGATTA CAAGATATAG TGATACGACG
    GCAGAAATAT TAGTGACTGT TATATTAAGA AATTTTGAAC AGCTACAAAC TAAAAGAACA
    GCATCGGATT CTCACTATGT TACCTTAATC ATAGGTGACG CAGATAATCA AGTAGTTTAT
    CTGCACAAGA TTACGGATTC TGTTTTGCTA AAAGCTGGAA GTTGGGCTAA AAAGATTGCT
    GTGAAAAGAG CTCTTAAATC TGAAGATCTT AGCATAAATC TAATAAGTTC TGAATTTGTT
    GGGCTTGATA TTCAGCAGAA ACTTACAGTC TTTTACTTAG AACCCAAGAG GTTTGGAAAT
    CAAATCACTA TGCAAAGAAA ATCTGAAACA CAGATTTCCC ATTCTAAACA TTCAGACATA
    TCTACAATAG CAGGACCTAA TAAAGGAACG ACTGCCAGCA AAAAACCTGG GAACCGAGAA
    TGCAATCATC TTTGTAAAAG TAAACATACA TGTGGACATG ACTGCTGTAA AATTGGAGTT
    GCACAGAAGT CAGAAATTAA AGAGTCAACA ATTTCTTCAT ATTTATCTGA TTTAAGAAAC
    AGGAATGCTG TTTCATCTGT TCCTCCAGTT AAACGTCTGA AGATACAGAT GAATAAATCT
    CAAAGTGTGG ACCTTAAAGA GTTTGGTTTT ACTCCAAAAC CTTCCCTTCC TAGTATATCA
    AGGTCAGAAT ATTTAAACAT ATCTGAATTG CCTATAATGG AGCAGTGGGA TCAGCCTGAA
    ATCTATGGAA AAGTTAGACA AGAACCATCT GAATATCAAG ACAAAGAAGT TTTGAATGTG
    AACTTTGAAT TGGGAAATGA AGTTTGGGAT GATTTTGATG ATGAAAACTT AGAAGTTACT
    AGCTTTTCAA CTGATACTGA GAAGACAAAA ATATCAGGAT TTGGAAACAC TTTGAGTTCA
    AGTACCAGGG GAAGTAAGCT ACCCCTTCAA GAGTCAAAGA GCAAATTCCA AAGAGAAATG
    TCAAACAGTT TTGTTTCATC ACATGAGATG TCGGATATTT CTTTATCAAA TTCTGCTATG
    CCCAAGTTCA GTGCATCCTC CATGACAAAA TTACCTCAAC AAGCCGGAAA TGCAGTTATT
    GTCCATTTTC AAGAAAGAAA ACCACAAAAT CTGTCACCAG AGATTGAGAA GCAATGCTTT
    ACTTTCTCTG AAAAAAACCC AAATTCTTCA AATTATAAAA AAGTGGATTT TTTTATTAGA
    AACAGTGAAT GTAAAAAGGA AGTTGATTTC AGTATGTATC ATCCTGATGA TGAAGCTGAT
    GAAATGAAGT CTTTATTGGG AATATTTGAT GGTATTTTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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