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PTCRA cDNA ORF clone, Homo sapiens (human)

Gene Symbol PTCRA
Entrez Gene ID 171558
Full Name pre T-cell antigen receptor alpha
Synonyms PT-ALPHA, PTA
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6p21.3

Summary The protein encoded by this gene is a single-pass type I membrane protein that is found in immmature but not mature T-cells. Along with TCRB and CD3 complex, the encoded protein forms the pre-T-cell receptor complex, which regulates early T-cell development. Four transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]. lac of sum
Disorder MIM:

606817


mRNA and Protein(s)

mRNA Protein Name
NM_001243170 NP_001230099 pre T-cell antigen receptor alpha isoform 4 precursor
XM_011514316 XP_011512618 pre T-cell antigen receptor alpha isoform X1
XM_011514317 XP_011512619 pre T-cell antigen receptor alpha isoform X2
NM_001243168 NP_001230097 pre T-cell antigen receptor alpha isoform 1 precursor
NM_001243169 NP_001230098 pre T-cell antigen receptor alpha isoform 3 precursor
NM_138296 NP_612153 pre T-cell antigen receptor alpha isoform 2 precursor



Homo sapiens (human) PTCRA NP_612153.2
Pan troglodytes (chimpanzee) PTCRA XP_003950895.1
Macaca mulatta (Rhesus monkey) PTCRA XP_001088951.1
Canis lupus familiaris (dog) PTCRA XP_005627422.1
Bos taurus (cattle) PTCRA NP_001069101.1
Mus musculus (house mouse) Ptcra NP_035325.1
Rattus norvegicus (Norway rat) Ptcra XP_003750700.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PTCRA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTCRA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu55835 NM_001243170 Homo sapiens pre T-cell antigen receptor alpha (PTCRA), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu77450 XM_011514316 PREDICTED: Homo sapiens pre T-cell antigen receptor alpha (PTCRA), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $139.30
$199.00
OHu77451 XM_011514317 PREDICTED: Homo sapiens pre T-cell antigen receptor alpha (PTCRA), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu18718 NM_001243168 Homo sapiens pre T-cell antigen receptor alpha (PTCRA), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu55895 NM_001243169 Homo sapiens pre T-cell antigen receptor alpha (PTCRA), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu29044
NM_138296 Homo sapiens pre T-cell antigen receptor alpha (PTCRA), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$154.50-$216.30
$309.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu55835
Clone ID Related Accession (Same CDS sequence) NM_001243170
Accession Version NM_001243170.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 525bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 09-FEB-2014
Organism Homo sapiens (human)
Product pre T-cell antigen receptor alpha isoform 4 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U36759.1, AF101436.1, BC153829.1 and AW014894.1. Transcript Variant: This variant (4) lacks an alternate in-frame exon and uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (4) lacks two internal segments compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AF101436.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGGCCGGTA CATGGCTGCT ACTTCTCCTG GCCCTTGGGT GTCCAGCCCT ACCCACAGGA 
GAGGCTTCTA CAGCCAGGAC CTGCCCCCAG GAGCCTCTCA GGGGGACACC GGGTGGGGCG
CTGTGGCTGG GGGTCCTGCG GCTGCTGCTC TTCAAGCTGC TGCTGTTTGA CCTGCTCCTG
ACCTGCAGCT GCCTGTGCGA CCCCGCGGGC CCGCTGCCTT CCCCCGCAAC CACCACCCGC
CTGCGAGCCC TCGGCTCCCA TCGACTGCAC CCGGCCACGG AGACTGGGGG ACGAGAGGCC
ACCAGCTCAC CCAGACCCCA GCCTCGGGAC CGCCGCTGGG GTGACACCCC TCCGGGTCGG
AAGCCCGGGA GCCCAGTATG GGGGGAAGGG TCTTACCTCA GCAGTTACCC CACTTGCCCA
GCACAGGCCT GGTGCTCAAG ATCTGCCCTC AGGGCTCCTT CCTCCAGTCT TGGAGCATTT
TTTGCAGGTG ACCTGCCTCC TCCTCTGCAG GCTGGAGCTG CCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001230099.1
CDS82..606
Misc Feature(1)1..3(+)
Misc Feature(2)124..279(+)
Exon (1)1..139
Gene:PTCRA
Gene Synonym:
Exon (2)140..184
Gene:PTCRA
Gene Synonym:
Exon (3)185..762
Gene:PTCRA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001243170
Organism Homo sapiens (human)
Definition Homo sapiens pre T-cell antigen receptor alpha (PTCRA), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001243170

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGCCGGTA CATGGCTGCT ACTTCTCCTG GCCCTTGGGT GTCCAGCCCT ACCCACAGGA 
GAGGCTTCTA CAGCCAGGAC CTGCCCCCAG GAGCCTCTCA GGGGGACACC GGGTGGGGCG
CTGTGGCTGG GGGTCCTGCG GCTGCTGCTC TTCAAGCTGC TGCTGTTTGA CCTGCTCCTG
ACCTGCAGCT GCCTGTGCGA CCCCGCGGGC CCGCTGCCTT CCCCCGCAAC CACCACCCGC
CTGCGAGCCC TCGGCTCCCA TCGACTGCAC CCGGCCACGG AGACTGGGGG ACGAGAGGCC
ACCAGCTCAC CCAGACCCCA GCCTCGGGAC CGCCGCTGGG GTGACACCCC TCCGGGTCGG
AAGCCCGGGA GCCCAGTATG GGGGGAAGGG TCTTACCTCA GCAGTTACCC CACTTGCCCA
GCACAGGCCT GGTGCTCAAG ATCTGCCCTC AGGGCTCCTT CCTCCAGTCT TGGAGCATTT
TTTGCAGGTG ACCTGCCTCC TCCTCTGCAG GCTGGAGCTG CCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu77450
Clone ID Related Accession (Same CDS sequence) XM_011514316
Accession Version XM_011514316.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 747bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pre T-cell antigen receptor alpha isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007592.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGCTGCTGG TGGATGGAAA GCAGCAGATG GTGGTGGTCT GCCTGGTCCT TGATGTTGCA 
CCCCCTGGCC TTGACAGCCC CATCTGGTTC TCAGCCGGCA ATGGCAGTGC ACTGGATGCC
TTCACCTATG GCCCTTCCCC AGCAACGGAT GGCACCTGGA CCAACTTGGC CCATCTCTCC
CTGCCTTCTG AGGAGCTGGC ATCCTGGGAG CCTTTGGTCT GCCACACTGG GCCTGGGGCT
GAGGGTCACA GCAGGAGTAC ACAGCCCATG CATCTGTCAG GAGAGGCTTC TACAGCCAGG
ACCTGCCCCC AGGAGCCTCT CAGGGGGACA CCGGGTGGGG CGCTGTGGCT GGGGGTCCTG
CGGCTGCTGC TCTTCAAGCT GCTGCTGTTT GACCTGCTCC TGACCTGCAG CTGCCTGTGC
GACCCCGCGG GCCCGCTGCC TTCCCCCGCA ACCACCACCC GCCTGCGAGC CCTCGGCTCC
CATCGACTGC ACCCGGCCAC GGAGACTGGG GGACGAGAGG CCACCAGCTC ACCCAGACCC
CAGCCTCGGG ACCGCCGCTG GGGTGACACC CCTCCGGGTC GGAAGCCCGG GAGCCCAGTA
TGGGGGGAAG GGTCTTACCT CAGCAGTTAC CCCACTTGCC CAGCACAGGC CTGGTGCTCA
AGATCTGCCC TCAGGGCTCC TTCCTCCAGT CTTGGAGCAT TTTTTGCAGG TGACCTGCCT
CCTCCTCTGC AGGCTGGAGC TGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011512618.1
CDS132..878
Misc Feature(1)132..551(+)
Translation

Target ORF information:

RefSeq Version XM_011514316
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens pre T-cell antigen receptor alpha (PTCRA), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011514316

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGCTGCTGG TGGATGGAAA GCAGCAGATG GTGGTGGTCT GCCTGGTCCT TGATGTTGCA 
CCCCCTGGCC TTGACAGCCC CATCTGGTTC TCAGCCGGCA ATGGCAGTGC ACTGGATGCC
TTCACCTATG GCCCTTCCCC AGCAACGGAT GGCACCTGGA CCAACTTGGC CCATCTCTCC
CTGCCTTCTG AGGAGCTGGC ATCCTGGGAG CCTTTGGTCT GCCACACTGG GCCTGGGGCT
GAGGGTCACA GCAGGAGTAC ACAGCCCATG CATCTGTCAG GAGAGGCTTC TACAGCCAGG
ACCTGCCCCC AGGAGCCTCT CAGGGGGACA CCGGGTGGGG CGCTGTGGCT GGGGGTCCTG
CGGCTGCTGC TCTTCAAGCT GCTGCTGTTT GACCTGCTCC TGACCTGCAG CTGCCTGTGC
GACCCCGCGG GCCCGCTGCC TTCCCCCGCA ACCACCACCC GCCTGCGAGC CCTCGGCTCC
CATCGACTGC ACCCGGCCAC GGAGACTGGG GGACGAGAGG CCACCAGCTC ACCCAGACCC
CAGCCTCGGG ACCGCCGCTG GGGTGACACC CCTCCGGGTC GGAAGCCCGG GAGCCCAGTA
TGGGGGGAAG GGTCTTACCT CAGCAGTTAC CCCACTTGCC CAGCACAGGC CTGGTGCTCA
AGATCTGCCC TCAGGGCTCC TTCCTCCAGT CTTGGAGCAT TTTTTGCAGG TGACCTGCCT
CCTCCTCTGC AGGCTGGAGC TGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu77451
Clone ID Related Accession (Same CDS sequence) XM_011514317
Accession Version XM_011514317.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 717bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pre T-cell antigen receptor alpha isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007592.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTGCTA CTTCTCCTGG CCCTTGGGTG TCCAGCCCTA CCCACAGCCC CATCTGGTTC 
TCAGCCGGCA ATGGCAGTGC ACTGGATGCC TTCACCTATG GCCCTTCCCC AGCAACGGAT
GGCACCTGGA CCAACTTGGC CCATCTCTCC CTGCCTTCTG AGGAGCTGGC ATCCTGGGAG
CCTTTGGTCT GCCACACTGG GCCTGGGGCT GAGGGTCACA GCAGGAGTAC ACAGCCCATG
CATCTGTCAG GAGAGGCTTC TACAGCCAGG ACCTGCCCCC AGGAGCCTCT CAGGGGGACA
CCGGGTGGGG CGCTGTGGCT GGGGGTCCTG CGGCTGCTGC TCTTCAAGCT GCTGCTGTTT
GACCTGCTCC TGACCTGCAG CTGCCTGTGC GACCCCGCGG GCCCGCTGCC TTCCCCCGCA
ACCACCACCC GCCTGCGAGC CCTCGGCTCC CATCGACTGC ACCCGGCCAC GGAGACTGGG
GGACGAGAGG CCACCAGCTC ACCCAGACCC CAGCCTCGGG ACCGCCGCTG GGGTGACACC
CCTCCGGGTC GGAAGCCCGG GAGCCCAGTA TGGGGGGAAG GGTCTTACCT CAGCAGTTAC
CCCACTTGCC CAGCACAGGC CTGGTGCTCA AGATCTGCCC TCAGGGCTCC TTCCTCCAGT
CTTGGAGCAT TTTTTGCAGG TGACCTGCCT CCTCCTCTGC AGGCTGGAGC TGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011512619.1
CDS275..991
Misc Feature(1)320..664(+)
Translation

Target ORF information:

RefSeq Version XM_011514317
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens pre T-cell antigen receptor alpha (PTCRA), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011514317

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTGCTA CTTCTCCTGG CCCTTGGGTG TCCAGCCCTA CCCACAGCCC CATCTGGTTC 
TCAGCCGGCA ATGGCAGTGC ACTGGATGCC TTCACCTATG GCCCTTCCCC AGCAACGGAT
GGCACCTGGA CCAACTTGGC CCATCTCTCC CTGCCTTCTG AGGAGCTGGC ATCCTGGGAG
CCTTTGGTCT GCCACACTGG GCCTGGGGCT GAGGGTCACA GCAGGAGTAC ACAGCCCATG
CATCTGTCAG GAGAGGCTTC TACAGCCAGG ACCTGCCCCC AGGAGCCTCT CAGGGGGACA
CCGGGTGGGG CGCTGTGGCT GGGGGTCCTG CGGCTGCTGC TCTTCAAGCT GCTGCTGTTT
GACCTGCTCC TGACCTGCAG CTGCCTGTGC GACCCCGCGG GCCCGCTGCC TTCCCCCGCA
ACCACCACCC GCCTGCGAGC CCTCGGCTCC CATCGACTGC ACCCGGCCAC GGAGACTGGG
GGACGAGAGG CCACCAGCTC ACCCAGACCC CAGCCTCGGG ACCGCCGCTG GGGTGACACC
CCTCCGGGTC GGAAGCCCGG GAGCCCAGTA TGGGGGGAAG GGTCTTACCT CAGCAGTTAC
CCCACTTGCC CAGCACAGGC CTGGTGCTCA AGATCTGCCC TCAGGGCTCC TTCCTCCAGT
CTTGGAGCAT TTTTTGCAGG TGACCTGCCT CCTCCTCTGC AGGCTGGAGC TGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18718
Clone ID Related Accession (Same CDS sequence) NM_001243168
Accession Version NM_001243168.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 891bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pre T-cell antigen receptor alpha isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U36759.1, AK130819.1, BC153829.1 and AW014894.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: BP367764.1, BP367764.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCCGGTA CATGGCTGCT ACTTCTCCTG GCCCTTGGGT GTCCAGCCCT ACCCACAGGT 
GTGGGCGGCA CACCCTTTCC TTCTCTGGCC CCACCAATCA TGCTGCTGGT GGATGGAAAG
CAGCAGATGG TGGTGGTCTG CCTGGTCCTT GATGTTGCAC CCCCTGGCCT TGACAGCCCC
ATCTGGTTCT CAGCCGGCAA TGGCAGTGCA CTGGATGCCT TCACCTATGG CCCTTCCCCA
GCAACGGATG GCACCTGGAC CAACTTGGCC CATCTCTCCC TGCCTTCTGA GGAGCTGGCA
TCCTGGGAGC CTTTGGTCTG CCACACTGGG CCTGGGGCTG AGGGTCACAG CAGGAGTACA
CAGCCCATGC ATCTGTCAGG AGAGGCTTCT ACAGCCAGGA CCTGCCCCCA GGAGCCTCTC
AGGGGGGGCT GCGGGCTCCT GCGGGCTCCT GAGCGGTTCC TCCTCGCAGG GACACCGGGT
GGGGCGCTGT GGCTGGGGGT CCTGCGGCTG CTGCTCTTCA AGCTGCTGCT GTTTGACCTG
CTCCTGACCT GCAGCTGCCT GTGCGACCCC GCGGGCCCGC TGCCTTCCCC CGCAACCACC
ACCCGCCTGC GAGCCCTCGG CTCCCATCGA CTGCACCCGG CCACGGAGAC TGGGGGACGA
GAGGCCACCA GCTCACCCAG ACCCCAGCCT CGGGACCGCC GCTGGGGTGA CACCCCTCCG
GGTCGGAAGC CCGGGAGCCC AGTATGGGGG GAAGGGTCTT ACCTCAGCAG TTACCCCACT
TGCCCAGCAC AGGCCTGGTG CTCAAGATCT GCCCTCAGGG CTCCTTCCTC CAGTCTTGGA
GCATTTTTTG CAGGTGACCT GCCTCCTCCT CTGCAGGCTG GAGCTGCCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001230097.1
CDS82..972
Misc Feature(1)1..3(+)
Misc Feature(2)139..645(+)
Exon (1)1..139
Gene:PTCRA
Gene Synonym:
Exon (2)140..460
Gene:PTCRA
Gene Synonym:
Exon (3)461..505
Gene:PTCRA
Gene Synonym:
Exon (4)506..1128
Gene:PTCRA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001243168
Organism Homo sapiens (human)
Definition Homo sapiens pre T-cell antigen receptor alpha (PTCRA), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001243168

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCCGGTA CATGGCTGCT ACTTCTCCTG GCCCTTGGGT GTCCAGCCCT ACCCACAGGT 
GTGGGCGGCA CACCCTTTCC TTCTCTGGCC CCACCAATCA TGCTGCTGGT GGATGGAAAG
CAGCAGATGG TGGTGGTCTG CCTGGTCCTT GATGTTGCAC CCCCTGGCCT TGACAGCCCC
ATCTGGTTCT CAGCCGGCAA TGGCAGTGCA CTGGATGCCT TCACCTATGG CCCTTCCCCA
GCAACGGATG GCACCTGGAC CAACTTGGCC CATCTCTCCC TGCCTTCTGA GGAGCTGGCA
TCCTGGGAGC CTTTGGTCTG CCACACTGGG CCTGGGGCTG AGGGTCACAG CAGGAGTACA
CAGCCCATGC ATCTGTCAGG AGAGGCTTCT ACAGCCAGGA CCTGCCCCCA GGAGCCTCTC
AGGGGGGGCT GCGGGCTCCT GCGGGCTCCT GAGCGGTTCC TCCTCGCAGG GACACCGGGT
GGGGCGCTGT GGCTGGGGGT CCTGCGGCTG CTGCTCTTCA AGCTGCTGCT GTTTGACCTG
CTCCTGACCT GCAGCTGCCT GTGCGACCCC GCGGGCCCGC TGCCTTCCCC CGCAACCACC
ACCCGCCTGC GAGCCCTCGG CTCCCATCGA CTGCACCCGG CCACGGAGAC TGGGGGACGA
GAGGCCACCA GCTCACCCAG ACCCCAGCCT CGGGACCGCC GCTGGGGTGA CACCCCTCCG
GGTCGGAAGC CCGGGAGCCC AGTATGGGGG GAAGGGTCTT ACCTCAGCAG TTACCCCACT
TGCCCAGCAC AGGCCTGGTG CTCAAGATCT GCCCTCAGGG CTCCTTCCTC CAGTCTTGGA
GCATTTTTTG CAGGTGACCT GCCTCCTCCT CTGCAGGCTG GAGCTGCCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu55895
Clone ID Related Accession (Same CDS sequence) NM_001243169
Accession Version NM_001243169.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 771bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pre T-cell antigen receptor alpha isoform 3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U36759.1, AF165312.1, BC153829.1 and AW014894.1. Transcript Variant: This variant (3) uses alternate in-frame splice junctions at the 5' ends of two exons and contains an alternate in-frame exon compared to variant 1. The resulting isoform (3) contains an alternate internal segment but lacks two others compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AF165312.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2152719 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCCGGTA CATGGCTGCT ACTTCTCCTG GCCCTTGGGT GTCCAGCCCT ACCCACAGGT 
CCTGTTTCCT TCCCTTCCTC ACCAGAGGCT GCCACAACAG GCCCCATCTG GTTCTCAGCC
GGCAATGGCA GTGCACTGGA TGCCTTCACC TATGGCCCTT CCCCAGCAAC GGATGGCACC
TGGACCAACT TGGCCCATCT CTCCCTGCCT TCTGAGGAGC TGGCATCCTG GGAGCCTTTG
GTCTGCCACA CTGGGCCTGG GGCTGAGGGT CACAGCAGGA GTACACAGCC CATGCATCTG
TCAGGAGAGG CTTCTACAGC CAGGACCTGC CCCCAGGAGC CTCTCAGGGG GACACCGGGT
GGGGCGCTGT GGCTGGGGGT CCTGCGGCTG CTGCTCTTCA AGCTGCTGCT GTTTGACCTG
CTCCTGACCT GCAGCTGCCT GTGCGACCCC GCGGGCCCGC TGCCTTCCCC CGCAACCACC
ACCCGCCTGC GAGCCCTCGG CTCCCATCGA CTGCACCCGG CCACGGAGAC TGGGGGACGA
GAGGCCACCA GCTCACCCAG ACCCCAGCCT CGGGACCGCC GCTGGGGTGA CACCCCTCCG
GGTCGGAAGC CCGGGAGCCC AGTATGGGGG GAAGGGTCTT ACCTCAGCAG TTACCCCACT
TGCCCAGCAC AGGCCTGGTG CTCAAGATCT GCCCTCAGGG CTCCTTCCTC CAGTCTTGGA
GCATTTTTTG CAGGTGACCT GCCTCCTCCT CTGCAGGCTG GAGCTGCCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001230098.1
CDS82..852
Misc Feature(1)1..3(+)
Misc Feature(2)184..525(+)
Exon (1)1..139
Gene:PTCRA
Gene Synonym:
Exon (2)140..182
Gene:PTCRA
Gene Synonym:
Exon (3)183..385
Gene:PTCRA
Gene Synonym:
Exon (4)386..430
Gene:PTCRA
Gene Synonym:
Exon (5)431..1008
Gene:PTCRA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001243169
Organism Homo sapiens (human)
Definition Homo sapiens pre T-cell antigen receptor alpha (PTCRA), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001243169

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCCGGTA CATGGCTGCT ACTTCTCCTG GCCCTTGGGT GTCCAGCCCT ACCCACAGGT 
CCTGTTTCCT TCCCTTCCTC ACCAGAGGCT GCCACAACAG GCCCCATCTG GTTCTCAGCC
GGCAATGGCA GTGCACTGGA TGCCTTCACC TATGGCCCTT CCCCAGCAAC GGATGGCACC
TGGACCAACT TGGCCCATCT CTCCCTGCCT TCTGAGGAGC TGGCATCCTG GGAGCCTTTG
GTCTGCCACA CTGGGCCTGG GGCTGAGGGT CACAGCAGGA GTACACAGCC CATGCATCTG
TCAGGAGAGG CTTCTACAGC CAGGACCTGC CCCCAGGAGC CTCTCAGGGG GACACCGGGT
GGGGCGCTGT GGCTGGGGGT CCTGCGGCTG CTGCTCTTCA AGCTGCTGCT GTTTGACCTG
CTCCTGACCT GCAGCTGCCT GTGCGACCCC GCGGGCCCGC TGCCTTCCCC CGCAACCACC
ACCCGCCTGC GAGCCCTCGG CTCCCATCGA CTGCACCCGG CCACGGAGAC TGGGGGACGA
GAGGCCACCA GCTCACCCAG ACCCCAGCCT CGGGACCGCC GCTGGGGTGA CACCCCTCCG
GGTCGGAAGC CCGGGAGCCC AGTATGGGGG GAAGGGTCTT ACCTCAGCAG TTACCCCACT
TGCCCAGCAC AGGCCTGGTG CTCAAGATCT GCCCTCAGGG CTCCTTCCTC CAGTCTTGGA
GCATTTTTTG CAGGTGACCT GCCTCCTCCT CTGCAGGCTG GAGCTGCCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu29044
Clone ID Related Accession (Same CDS sequence) NM_138296
Accession Version NM_138296.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 846bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pre T-cell antigen receptor alpha isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U36759.1, BC100771.1, U38996.1 and AW014894.1. On Jul 7, 2006 this sequence version replaced gi:19923720. Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) lacks an alternate internal segment compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: U36759.1, BC100773.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCCGGTA CATGGCTGCT ACTTCTCCTG GCCCTTGGGT GTCCAGCCCT ACCCACAGGT 
GTGGGCGGCA CACCCTTTCC TTCTCTGGCC CCACCAATCA TGCTGCTGGT GGATGGAAAG
CAGCAGATGG TGGTGGTCTG CCTGGTCCTT GATGTTGCAC CCCCTGGCCT TGACAGCCCC
ATCTGGTTCT CAGCCGGCAA TGGCAGTGCA CTGGATGCCT TCACCTATGG CCCTTCCCCA
GCAACGGATG GCACCTGGAC CAACTTGGCC CATCTCTCCC TGCCTTCTGA GGAGCTGGCA
TCCTGGGAGC CTTTGGTCTG CCACACTGGG CCTGGGGCTG AGGGTCACAG CAGGAGTACA
CAGCCCATGC ATCTGTCAGG AGAGGCTTCT ACAGCCAGGA CCTGCCCCCA GGAGCCTCTC
AGGGGGACAC CGGGTGGGGC GCTGTGGCTG GGGGTCCTGC GGCTGCTGCT CTTCAAGCTG
CTGCTGTTTG ACCTGCTCCT GACCTGCAGC TGCCTGTGCG ACCCCGCGGG CCCGCTGCCT
TCCCCCGCAA CCACCACCCG CCTGCGAGCC CTCGGCTCCC ATCGACTGCA CCCGGCCACG
GAGACTGGGG GACGAGAGGC CACCAGCTCA CCCAGACCCC AGCCTCGGGA CCGCCGCTGG
GGTGACACCC CTCCGGGTCG GAAGCCCGGG AGCCCAGTAT GGGGGGAAGG GTCTTACCTC
AGCAGTTACC CCACTTGCCC AGCACAGGCC TGGTGCTCAA GATCTGCCCT CAGGGCTCCT
TCCTCCAGTC TTGGAGCATT TTTTGCAGGT GACCTGCCTC CTCCTCTGCA GGCTGGAGCT
GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_612153.2
CDS82..927
Misc Feature(1)1..3(+)
Misc Feature(2)139..600(+)
Exon (1)1..139
Gene:PTCRA
Gene Synonym:
Exon (2)140..460
Gene:PTCRA
Gene Synonym:
Exon (3)461..505
Gene:PTCRA
Gene Synonym:
Exon (4)506..1083
Gene:PTCRA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_138296
Organism Homo sapiens (human)
Definition Homo sapiens pre T-cell antigen receptor alpha (PTCRA), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_138296

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCCGGTA CATGGCTGCT ACTTCTCCTG GCCCTTGGGT GTCCAGCCCT ACCCACAGGT 
GTGGGCGGCA CACCCTTTCC TTCTCTGGCC CCACCAATCA TGCTGCTGGT GGATGGAAAG
CAGCAGATGG TGGTGGTCTG CCTGGTCCTT GATGTTGCAC CCCCTGGCCT TGACAGCCCC
ATCTGGTTCT CAGCCGGCAA TGGCAGTGCA CTGGATGCCT TCACCTATGG CCCTTCCCCA
GCAACGGATG GCACCTGGAC CAACTTGGCC CATCTCTCCC TGCCTTCTGA GGAGCTGGCA
TCCTGGGAGC CTTTGGTCTG CCACACTGGG CCTGGGGCTG AGGGTCACAG CAGGAGTACA
CAGCCCATGC ATCTGTCAGG AGAGGCTTCT ACAGCCAGGA CCTGCCCCCA GGAGCCTCTC
AGGGGGACAC CGGGTGGGGC GCTGTGGCTG GGGGTCCTGC GGCTGCTGCT CTTCAAGCTG
CTGCTGTTTG ACCTGCTCCT GACCTGCAGC TGCCTGTGCG ACCCCGCGGG CCCGCTGCCT
TCCCCCGCAA CCACCACCCG CCTGCGAGCC CTCGGCTCCC ATCGACTGCA CCCGGCCACG
GAGACTGGGG GACGAGAGGC CACCAGCTCA CCCAGACCCC AGCCTCGGGA CCGCCGCTGG
GGTGACACCC CTCCGGGTCG GAAGCCCGGG AGCCCAGTAT GGGGGGAAGG GTCTTACCTC
AGCAGTTACC CCACTTGCCC AGCACAGGCC TGGTGCTCAA GATCTGCCCT CAGGGCTCCT
TCCTCCAGTC TTGGAGCATT TTTTGCAGGT GACCTGCCTC CTCCTCTGCA GGCTGGAGCT
GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Spontaneous preterm birth in African Americans is associated with infection and inflammatory response gene variants
Am. J. Obstet. Gynecol. 200 (2), 209 (2009)
Velez DR, Fortunato S, Thorsen P, Lombardi SJ, Williams SM and Menon R.


book

Racial disparity in pathophysiologic pathways of preterm birth based on genetic variants
Reprod. Biol. Endocrinol. 7, 62 (2009)
Menon R, Pearce B, Velez DR, Merialdi M, Williams SM, Fortunato SJ and Thorsen P.


book

Preterm birth in Caucasians is associated with coagulation and inflammation pathway gene variants
PLoS ONE 3 (9), E3283 (2008)
Velez DR, Fortunato SJ, Thorsen P, Lombardi SJ, Williams SM and Menon R.


book

Identification of CMS as a cytosolic adaptor of the human pTalpha chain involved in pre-TCR function
Blood 110 (13), 4331-4340 (2007)
Navarro MN, Nusspaumer G, Fuentes P, Gonzalez-Garcia S, Alcain J and Toribio ML.


book

Extrathymic TCR gene rearrangement in human small intestine: identification of new splice forms of recombination activating gene-1 mRNA with selective tissue expression
J. Immunol. 171 (7), 3359-3371 (2003)
Bas A, Hammarstrom SG and Hammarstrom ML.


book

A critical role for the cytoplasmic tail of pTalpha in T lymphocyte development
Nat. Immunol. 3 (5), 483-488 (2002)
Aifantis I, Borowski C, Gounari F, Lacorazza HD, Nikolich-Zugich J and von Boehmer H.


book

Combined expression of pTalpha and Notch3 in T cell leukemia identifies the requirement of preTCR for leukemogenesis
Proc. Natl. Acad. Sci. U.S.A. 99 (6), 3788-3793 (2002)
Bellavia D, Campese AF, Checquolo S, Balestri A, Biondi A, Cazzaniga G, Lendahl U, Fehling HJ, Hayday AC, Frati L, von Boehmer H, Gulino A and Screpanti I.


book

Genomic structure of the human pre-T cell receptor alpha chain and expression of two mRNA isoforms
Eur. J. Immunol. 28 (11), 3824-3831 (1998)
Saint-Ruf C, Lechner O, Feinberg J and von Boehmer H.


book

Regulation of pre-T cell receptor (pT alpha-TCR beta) gene expression during human thymic development
J. Exp. Med. 184 (2), 519-530 (1996)
Ramiro AR, Trigueros C, Marquez C, San Millan JL and Toribio ML.


book

Cloning and comparative analysis of the human pre-T-cell receptor alpha-chain gene
Proc. Natl. Acad. Sci. U.S.A. 92 (26), 12105-12109 (1995)
Del Porto P, Bruno L, Mattei MG, von Boehmer H and Saint-Ruf C.


 
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