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AGXT cDNA ORF clone, Homo sapiens (human)

Gene Symbol AGXT
Entrez Gene ID 189
Full Name alanine-glyoxylate aminotransferase
Synonyms AGT, AGT1, AGXT1, PH1, SPAT, SPT, TLH6
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2q37.3

Summary This gene is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification. Mutations in this gene, some of which alter subcellular targetting, have been associated with type I primary hyperoxaluria. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

604285

Disorder Html: Hyperoxaluria, primary, type 1, 259900 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000030 NP_000021 serine--pyruvate aminotransferase



Homo sapiens (human) AGXT NP_000021.1
Pan troglodytes (chimpanzee) AGXT XP_003309627.2
Macaca mulatta (Rhesus monkey) AGXT XP_001090301.2
Canis lupus familiaris (dog) LOC100855679 XP_003639939.1
Canis lupus familiaris (dog) LOC607355 XP_848328.2
Bos taurus (cattle) AGXT NP_001095825.1
Mus musculus (house mouse) Agxt NP_057911.2
Rattus norvegicus (Norway rat) Agxt NP_085914.2
Gallus gallus (chicken) AGXT XP_003641783.2
Danio rerio (zebrafish) agxta NP_001002331.1
Danio rerio (zebrafish) agxtb NP_998327.1
Drosophila melanogaster (fruit fly) Spat NP_511062.1
Caenorhabditis elegans T14D7.1 NP_495885.1
Arabidopsis thaliana (thale cress) AGT NP_178969.1
Xenopus (Silurana) tropicalis (western clawed frog) agxt NP_001006705.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AGXT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGXT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu18906 NM_000030 Homo sapiens alanine-glyoxylate aminotransferase (AGXT), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18906
Clone ID Related Accession (Same CDS sequence) NM_000030
Accession Version NM_000030.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1179bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product serine--pyruvate aminotransferase
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X53414.1, AA857309.1 and CB156891.1. This sequence is a reference standard in the RefSeqGene project. On Apr 2, 2008 this sequence version replaced gi:4557288. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X53414.1, BC132819.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGCCTCTC ACAAGCTGCT GGTGACCCCC CCCAAGGCCC TGCTCAAGCC CCTCTCCATC 
CCCAACCAGC TCCTGCTGGG GCCTGGTCCT TCCAACCTGC CTCCTCGCAT CATGGCAGCC
GGGGGGCTGC AGATGATCGG GTCCATGAGC AAGGATATGT ACCAGATCAT GGACGAGATC
AAGGAAGGCA TCCAGTACGT GTTCCAGACC AGGAACCCAC TCACACTGGT CATCTCTGGC
TCGGGACACT GTGCCCTGGA GGCCGCCCTG GTCAATGTGC TGGAGCCTGG GGACTCCTTC
CTGGTTGGGG CCAATGGCAT TTGGGGGCAG CGAGCCGTGG ACATCGGGGA GCGCATAGGA
GCCCGAGTGC ACCCGATGAC CAAGGACCCT GGAGGCCACT ACACACTGCA GGAGGTGGAG
GAGGGCCTGG CCCAGCACAA GCCAGTGCTG CTGTTCTTAA CCCACGGGGA GTCGTCCACC
GGCGTGCTGC AGCCCCTTGA TGGCTTCGGG GAACTCTGCC ACAGGTACAA GTGCCTGCTC
CTGGTGGATT CGGTGGCATC CCTGGGCGGG ACCCCCCTTT ACATGGACCG GCAAGGCATC
GACATCCTGT ACTCGGGCTC CCAGAAGGCC CTGAACGCCC CTCCAGGGAC CTCGCTCATC
TCCTTCAGTG ACAAGGCCAA AAAGAAGATG TACTCCCGCA AGACGAAGCC CTTCTCCTTC
TACCTGGACA TCAAGTGGCT GGCCAACTTC TGGGGCTGTG ACGACCAGCC CAGGATGTAC
CATCACACAA TCCCCGTCAT CAGCCTGTAC AGCCTGAGAG AGAGCCTGGC CCTCATTGCG
GAACAGGGCC TGGAGAACAG CTGGCGCCAG CACCGCGAGG CCGCGGCGTA TCTGCATGGG
CGCCTGCAGG CACTGGGGCT GCAGCTCTTC GTGAAGGACC CGGCGCTCCG GCTTCCCACA
GTCACCACTG TGGCTGTACC CGCTGGCTAT GACTGGAGAG ACATCGTCAG CTACGTCATA
GACCACTTCG ACATTGAGAT CATGGGTGGC CTTGGGCCCT CCACGGGGAA GGTGCTGCGG
ATCGGCCTGC TGGGCTGCAA TGCCACCCGC GAGAATGTGG ACCGCGTGAC GGAGGCCCTG
AGGGCGGCCC TGCAGCACTG CCCCAAGAAG AAGCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000021.1
CDS122..1300
Misc Feature(1)182..1273(+)
Misc Feature(2)191..1276(+)
Misc Feature(3)233..1141(+)
Misc Feature(4)362..748(+)
Misc Feature(5)746..748(+)
Misc Feature(6)746..748(+)
Exon (1)1..286
Gene:AGXT
Gene Synonym:
Exon (2)287..479
Gene:AGXT
Gene Synonym:
Exon (3)480..544
Gene:AGXT
Gene Synonym:
Exon (4)545..645
Gene:AGXT
Gene Synonym:
Exon (5)646..716
Gene:AGXT
Gene Synonym:
Exon (6)717..801
Gene:AGXT
Gene Synonym:
Exon (7)802..897
Gene:AGXT
Gene Synonym:
Exon (8)898..967
Gene:AGXT
Gene Synonym:
Exon (9)968..1063
Gene:AGXT
Gene Synonym:
Exon (10)1064..1192
Gene:AGXT
Gene Synonym:
Exon (11)1193..1598
Gene:AGXT
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000030
Organism Homo sapiens (human)
Definition Homo sapiens alanine-glyoxylate aminotransferase (AGXT), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000030

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGCCTCTC ACAAGCTGCT GGTGACCCCC CCCAAGGCCC TGCTCAAGCC CCTCTCCATC 
CCCAACCAGC TCCTGCTGGG GCCTGGTCCT TCCAACCTGC CTCCTCGCAT CATGGCAGCC
GGGGGGCTGC AGATGATCGG GTCCATGAGC AAGGATATGT ACCAGATCAT GGACGAGATC
AAGGAAGGCA TCCAGTACGT GTTCCAGACC AGGAACCCAC TCACACTGGT CATCTCTGGC
TCGGGACACT GTGCCCTGGA GGCCGCCCTG GTCAATGTGC TGGAGCCTGG GGACTCCTTC
CTGGTTGGGG CCAATGGCAT TTGGGGGCAG CGAGCCGTGG ACATCGGGGA GCGCATAGGA
GCCCGAGTGC ACCCGATGAC CAAGGACCCT GGAGGCCACT ACACACTGCA GGAGGTGGAG
GAGGGCCTGG CCCAGCACAA GCCAGTGCTG CTGTTCTTAA CCCACGGGGA GTCGTCCACC
GGCGTGCTGC AGCCCCTTGA TGGCTTCGGG GAACTCTGCC ACAGGTACAA GTGCCTGCTC
CTGGTGGATT CGGTGGCATC CCTGGGCGGG ACCCCCCTTT ACATGGACCG GCAAGGCATC
GACATCCTGT ACTCGGGCTC CCAGAAGGCC CTGAACGCCC CTCCAGGGAC CTCGCTCATC
TCCTTCAGTG ACAAGGCCAA AAAGAAGATG TACTCCCGCA AGACGAAGCC CTTCTCCTTC
TACCTGGACA TCAAGTGGCT GGCCAACTTC TGGGGCTGTG ACGACCAGCC CAGGATGTAC
CATCACACAA TCCCCGTCAT CAGCCTGTAC AGCCTGAGAG AGAGCCTGGC CCTCATTGCG
GAACAGGGCC TGGAGAACAG CTGGCGCCAG CACCGCGAGG CCGCGGCGTA TCTGCATGGG
CGCCTGCAGG CACTGGGGCT GCAGCTCTTC GTGAAGGACC CGGCGCTCCG GCTTCCCACA
GTCACCACTG TGGCTGTACC CGCTGGCTAT GACTGGAGAG ACATCGTCAG CTACGTCATA
GACCACTTCG ACATTGAGAT CATGGGTGGC CTTGGGCCCT CCACGGGGAA GGTGCTGCGG
ATCGGCCTGC TGGGCTGCAA TGCCACCCGC GAGAATGTGG ACCGCGTGAC GGAGGCCCTG
AGGGCGGCCC TGCAGCACTG CCCCAAGAAG AAGCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

A double mutation in AGXT gene in families with primary hyperoxaluria type 1
Gene 531 (2), 451-456 (2013)
Kanoun H, Jarraya F, Hadj Salem I, Mahfoudh H, Chaabouni Y, Makni F, Hachicha J and Fakhfakh F.


book

Gly161 mutations associated with Primary Hyperoxaluria Type I induce the cytosolic aggregation and the intracellular degradation of the apo-form of alanine:glyoxylate aminotransferase
Biochim. Biophys. Acta 1832 (12), 2277-2288 (2013)
Oppici E, Roncador A, Montioli R, Bianconi S and Cellini B.


book

Mutation spectrum of primary hyperoxaluria type 1 in Tunisia: implication for diagnosis in North Africa
Gene 527 (1), 316-320 (2013)
Nagara M, Tiar A, Ben Halim N, Ben Rhouma F, Messaoud O, Bouyacoub Y, Kefi R, Hassayoun S, Zouari N, Ben Ammar MS, Abdelhak S and Chemli J.


book

Protein homeostasis defects of alanine-glyoxylate aminotransferase: new therapeutic strategies in primary hyperoxaluria type I
Biomed Res Int 2013, 687658 (2013)
Pey AL, Albert A and Salido E.


book

Evolution of alanine:glyoxylate aminotransferase 1 peroxisomal and mitochondrial targeting. A survey of its subcellular distribution in the livers of various representatives of the classes Mammalia, Aves and Amphibia
Eur. J. Cell Biol. 64 (2), 295-313 (1994)
Danpure CJ, Fryer P, Jennings PR, Allsop J, Griffiths S and Cunningham A.


book

Primary Hyperoxaluria Type 1
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Coulter-Mackie,M.B., White,C.T., Hurley,R.M., Chew,B.H. and Lange,D.


book

A serine-to-phenylalanine substitution leads to loss of alanine:glyoxylate aminotransferase catalytic activity and immunoreactivity in a patient with primary hyperoxaluria type 1
Hum. Mol. Genet. 1 (8), 643-644 (1992)
Minatogawa Y, Tone S, Allsop J, Purdue PE, Takada Y, Danpur CJ and Kido R.


book

A glycine-to-glutamate substitution abolishes alanine:glyoxylate aminotransferase catalytic activity in a subset of patients with primary hyperoxaluria type 1
Genomics 13 (1), 215-218 (1992)
Purdue PE, Lumb MJ, Allsop J, Minatogawa Y and Danpure CJ.


book

Mistargeting of peroxisomal L-alanine:glyoxylate aminotransferase to mitochondria in primary hyperoxaluria patients depends upon activation of a cryptic mitochondrial targeting sequence by a point mutation
Proc. Natl. Acad. Sci. U.S.A. 88 (23), 10900-10904 (1991)
Purdue PE, Allsop J, Isaya G, Rosenberg LE and Danpure CJ.


book

Identification of mutations associated with peroxisome-to-mitochondrion mistargeting of alanine/glyoxylate aminotransferase in primary hyperoxaluria type 1
J. Cell Biol. 111 (6 PT 1), 2341-2351 (1990)
Purdue PE, Takada Y and Danpure CJ.


 
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