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EGFR cDNA ORF clone, Homo sapiens(Human)

Gene Symbol EGFR
Entrez Gene ID 1956
Full Name epidermal growth factor receptor
Synonyms ERBB,ERBB1,HER1,NISBD2,PIG61,mENA
General protein information
Preferred Names

epidermal growth factor receptor

Names

epidermal growth factor receptor
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
cell growth inhibiting protein 40
cell proliferation-inducing protein 61
epidermal growth factor receptor tyrosine kinase domain
erb-b2 receptor tyrosine kinase 1
proto-oncogene c-ErbB-1
receptor tyrosine-protein kinase erbB-1

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

7

7p11.2

Summary The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016].
Disorder MIM:

131550

Disorder Html: Nonsmall cell lung cancer, response to tyrosine kinase inhibitor in,

mRNA and Protein(s)

mRNA Protein Name
NM_005228.3 NP_005219.2 epidermal growth factor receptor isoform a precursor
NM_201282.1 NP_958439.1 epidermal growth factor receptor isoform b precursor
NM_001346897.1 NP_001333826.1 epidermal growth factor receptor isoform e precursor
NM_005228.4 NP_005219.2 epidermal growth factor receptor isoform a precursor
NM_001346898.1 NP_001333827.1 epidermal growth factor receptor isoform f precursor
NM_001346941.1 NP_001333870.1 epidermal growth factor receptor isoform i precursor
NM_001346900.1 NP_001333829.1 epidermal growth factor receptor isoform h
NM_201283.1 NP_958440.1 epidermal growth factor receptor isoform c precursor
NM_001346899.1 NP_001333828.1 epidermal growth factor receptor isoform g precursor
NM_201284.1 NP_958441.1 epidermal growth factor receptor isoform d precursor



Canis lupus familiaris (dog) EGFR XP_533073.3
Pan troglodytes (chimpanzee) EGFR XP_519102.3
Bos taurus (cattle) EGFR XP_002696936.2
Mus musculus (house mouse) Egfr NP_997538.1
Gallus gallus (chicken) EGFR NP_990828.2
Danio rerio (zebrafish) egfra NP_919405.1
Macaca mulatta (rhesus monkey) EGFR XP_001107305.1
Rattus norvegicus (Norway rat) Egfr NP_113695.1
Homo sapiens (human) EGFR NP_005219.2
Xenopus tropicalis (tropical clawed frog) egfr XP_002939960.2


Related articles in PubMed

Epidermal growth factor receptor mutations should be considered as a prognostic factor for survival of patients with pathological fractures or painful bone metastases from non-small cell lung cancer.
Willeumier JJ, van der Hoeven NM, Bollen L, Willems LN, Fiocco M, van der Linden YM, Dijkstra PD
The bone & joint journal99-B(4)516-521(2017 Apr)

Impact of epidermal growth factor receptor gene expression level on clinical outcomes in epidermal growth factor receptor mutant lung adenocarcinoma patients taking first-line epidermal growth factor receptor-tyrosine kinase inhibitors.
Chang HC, Chen YM, Tseng CC, Huang KT, Wang CC, Chen YC, Lai CH, Fang WF, Kao HC, Lin MC
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(3)1010428317695939(2017 Mar)

Correlation of EGFR or KRAS mutation status with 18F-FDG uptake on PET-CT scan in lung adenocarcinoma.
Takamochi K, Mogushi K, Kawaji H, Imashimizu K, Fukui M, Oh S, Itoh M, Hayashizaki Y, Ko W, Akeboshi M, Suzuki K
PloS one12(4)e0175622(2017)

Expression, Mutation, and Amplification Status of EGFR and Its Correlation with Five miRNAs in Salivary Gland Tumours.
Boštjančič E, Hauptman N, Grošelj A, Glavač D, Volavšek M
BioMed research international20179150402(2017)

The epidermal growth factor receptor variant III (EGFRvIII): where wild things are altered.
Gan HK, Cvrljevic AN, Johns TG
The FEBS journal280(21)5350-70(2013 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We confirmed the partial responsibility of p-Akt activation in invasion of some bladder cancer cell lines expressing N-cadherin or p-EGFR
Title: Phospho-Akt pathway activation and inhibition depends on N-cadherin or phospho-EGFR expression in invasive human bladder cancer cell lines.

Results revealed significantly increased levels of epidermal growth factor in both serum and bronco-alveolar lavage.
Title: Serum levels of epidermal growth factor and HER-2 neu in non small-cell lung cancer: prognostic correlation.

increase in both gene expression and gene copy number in Chinese patients with gastric carcinoma; likely due to chromosome 7 polysomy
Title: Analysis of EGFR, HER2, and TOP2A gene status and chromosomal polysomy in gastric adenocarcinoma from Chinese patients.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in apoptosis and immunoregulation-related genes are associated with risk of chronic lymphocytic leukemia.

Observational study and meta-analysis of gene-disease association. (HuGE Navigator)
Title: Polymorphism in the IL18 gene and epithelial ovarian cancer in non-Hispanic white women.

The following EGFR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EGFR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu25365 NM_201282.1
Latest version!
Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu25409 NM_201283.1
Latest version!
Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$169.50-$237.30
$339.00
OHu25437 NM_005228.3
Latest version!
Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$609.50-$853.30
$1219.00
OHu25398 NM_201284.1
Latest version!
Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $496.30
$709.00
OHu102763 NM_001346941.1
Latest version!
Homo sapiens epidermal growth factor receptor (EGFR), transcript variant EGFRvIII, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $475.30
$679.00
OHu102753 NM_001346900.1
Latest version!
Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 15-17 $559.30
$799.00
OHu102757 NM_005228.4
Latest version!
Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 15-17 $643.30
$919.00
OHu102730 NM_001346899.1
Latest version!
Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $559.30
$799.00
OHu102762 NM_001346898.1
Latest version!
Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $559.30
$799.00
OHu102780 NM_001346897.1
Latest version!
Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $559.30
$799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu25365
Clone ID Related Accession (Same CDS sequence) NM_201282.1
Accession Version NM_201282.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1887bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product epidermal growth factor receptor isoform b precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AW163038.1, X00588.1, AF125253.1 and AF277897.1. Transcript Variant: This variant (2) uses a different 3' terminal exon when compared to variant 1. The resulting isoform (b) has a shorter and distinct C-terminus. Only the extracellular domain is present in isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 11161793 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
CATCCAAACT GCACCTACGG GTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_958439.1
CDS247..2133
Misc Feature(1)208..210
Misc Feature(2)order(337..339,418..420)
Misc Feature(3)412..414
Misc Feature(4)415..750
Misc Feature(5)469..1146
Misc Feature(6)628..630
Misc Feature(7)order(715..717,805..807)
Misc Feature(8)769..771
Misc Feature(9)799..1251
Misc Feature(10)order(814..816,841..843)
Misc Feature(11)order(826..828,865..867)
Misc Feature(12)832..834
Misc Feature(13)order(889..891,913..915)
Misc Feature(14)order(901..903,937..939)
Misc Feature(15)931..933
Misc Feature(16)937..1068
Misc Feature(17)order(940..942,964..966)
Misc Feature(18)order(952..954,988..990)
Misc Feature(19)order(997..999,1024..1026)
Misc Feature(20)order(1036..1038,1117..1119)
Misc Feature(21)order(1129..1131,1165..1167)
Misc Feature(22)order(1177..1179,1222..1224)
Misc Feature(23)order(1231..1233,1243..1245)
Misc Feature(24)order(1255..1257,1330..1332)
Misc Feature(25)1300..1302
Misc Feature(26)1327..1689
Misc Feature(27)1327..1329
Misc Feature(28)1414..2046
Misc Feature(29)1483..1485
Misc Feature(30)1576..1578
Misc Feature(31)order(1654..1656,1741..1743)
Misc Feature(32)1759..2127
Misc Feature(33)1762..1923
Misc Feature(34)order(1762..1764,1789..1791)
Misc Feature(35)order(1774..1776,1813..1815)
Misc Feature(36)order(1822..1824,1849..1851)
Misc Feature(37)1828..1830
Misc Feature(38)order(1861..1863,1909..1911)
Misc Feature(39)1900..2040
Misc Feature(40)order(1918..1920,1957..1959)
Misc Feature(41)order(1930..1932,1981..1983)
Misc Feature(42)1948..1950
Misc Feature(43)order(1990..1992,2017..2019)
Misc Feature(44)order(2029..2031,2095..2097)
Misc Feature(45)2053..2055
Misc Feature(46)2113..2115
Exon (1)1..334
Exon (2)335..486
Exon (3)487..670
Exon (4)671..805
Exon (5)806..874
Exon (6)875..993
Exon (7)994..1135
Exon (8)1136..1252
Exon (9)1253..1379
Exon (10)1380..1453
Exon (11)1454..1544
Exon (12)1545..1744
Exon (13)1745..1877
Exon (14)1878..1968
Exon (15)1969..2126
Exon (16)2127..2239
Translation

Target ORF information:

RefSeq Version NM_201282.1
Organism Homo sapiens(Human)
Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_201282.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
CATCCAAACT GCACCTACGG GTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25409
Clone ID Related Accession (Same CDS sequence) NM_201283.1
Accession Version NM_201283.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1218bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product epidermal growth factor receptor isoform c precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AW163038.1, X00588.1 and U48722.1. Transcript Variant: This variant (3, also known as ErbB1-S) uses a different 3' terminal exon when compared to variant 1. The resulting isoform (c) has a shorter and distinct C-terminus. Only the extracellular domain is present in isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U48722.1, AY698024.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
ATCACAGGTT TGAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_958440.1
CDS247..1464
Misc Feature(1)208..210
Misc Feature(2)order(337..339,418..420)
Misc Feature(3)412..414
Misc Feature(4)415..750
Misc Feature(5)469..1146
Misc Feature(6)628..630
Misc Feature(7)order(715..717,805..807)
Misc Feature(8)769..771
Misc Feature(9)799..1251
Misc Feature(10)order(814..816,841..843)
Misc Feature(11)order(826..828,865..867)
Misc Feature(12)832..834
Misc Feature(13)order(889..891,913..915)
Misc Feature(14)order(901..903,937..939)
Misc Feature(15)931..933
Misc Feature(16)937..1068
Misc Feature(17)order(940..942,964..966)
Misc Feature(18)order(952..954,988..990)
Misc Feature(19)order(997..999,1024..1026)
Misc Feature(20)order(1036..1038,1117..1119)
Misc Feature(21)order(1129..1131,1165..1167)
Misc Feature(22)order(1177..1179,1222..1224)
Misc Feature(23)order(1231..1233,1243..1245)
Misc Feature(24)order(1255..1257,1330..1332)
Misc Feature(25)1300..1302
Misc Feature(26)1327..>1455
Misc Feature(27)1327..1329
Exon (1)1..334
Exon (2)335..486
Exon (3)487..670
Exon (4)671..805
Exon (5)806..874
Exon (6)875..993
Exon (7)994..1135
Exon (8)1136..1252
Exon (9)1253..1379
Exon (10)1380..1572
Translation

Target ORF information:

RefSeq Version NM_201283.1
Organism Homo sapiens(Human)
Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_201283.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
ATCACAGGTT TGAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25437
Clone ID Related Accession (Same CDS sequence) NM_005228.3
Accession Version NM_005228.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-15
Organism Homo sapiens(Human)
Product epidermal growth factor receptor isoform a precursor
Comment

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
CATCCAAACT GCACCTACGG ATGCACTGGG CCAGGTCTTG AAGGCTGTCC AACGAATGGG
CCTAAGATCC CGTCCATCGC CACTGGGATG GTGGGGGCCC TCCTCTTGCT GCTGGTGGTG
GCCCTGGGGA TCGGCCTCTT CATGCGAAGG CGCCACATCG TTCGGAAGCG CACGCTGCGG
AGGCTGCTGC AGGAGAGGGA GCTTGTGGAG CCTCTTACAC CCAGTGGAGA AGCTCCCAAC
CAAGCTCTCT TGAGGATCTT GAAGGAAACT GAATTCAAAA AGATCAAAGT GCTGGGCTCC
GGTGCGTTCG GCACGGTGTA TAAGGGACTC TGGATCCCAG AAGGTGAGAA AGTTAAAATT
CCCGTCGCTA TCAAGGAATT AAGAGAAGCA ACATCTCCGA AAGCCAACAA GGAAATCCTC
GATGAAGCCT ACGTGATGGC CAGCGTGGAC AACCCCCACG TGTGCCGCCT GCTGGGCATC
TGCCTCACCT CCACCGTGCA GCTCATCACG CAGCTCATGC CCTTCGGCTG CCTCCTGGAC
TATGTCCGGG AACACAAAGA CAATATTGGC TCCCAGTACC TGCTCAACTG GTGTGTGCAG
ATCGCAAAGG GCATGAACTA CTTGGAGGAC CGTCGCTTGG TGCACCGCGA CCTGGCAGCC
AGGAACGTAC TGGTGAAAAC ACCGCAGCAT GTCAAGATCA CAGATTTTGG GCTGGCCAAA
CTGCTGGGTG CGGAAGAGAA AGAATACCAT GCAGAAGGAG GCAAAGTGCC TATCAAGTGG
ATGGCATTGG AATCAATTTT ACACAGAATC TATACCCACC AGAGTGATGT CTGGAGCTAC
GGGGTGACCG TTTGGGAGTT GATGACCTTT GGATCCAAGC CATATGACGG AATCCCTGCC
AGCGAGATCT CCTCCATCCT GGAGAAAGGA GAACGCCTCC CTCAGCCACC CATATGTACC
ATCGATGTCT ACATGATCAT GGTCAAGTGC TGGATGATAG ACGCAGATAG TCGCCCAAAG
TTCCGTGAGT TGATCATCGA ATTCTCCAAA ATGGCCCGAG ACCCCCAGCG CTACCTTGTC
ATTCAGGGGG ATGAAAGAAT GCATTTGCCA AGTCCTACAG ACTCCAACTT CTACCGTGCC
CTGATGGATG AAGAAGACAT GGACGACGTG GTGGATGCCG ACGAGTACCT CATCCCACAG
CAGGGCTTCT TCAGCAGCCC CTCCACGTCA CGGACTCCCC TCCTGAGCTC TCTGAGTGCA
ACCAGCAACA ATTCCACCGT GGCTTGCATT GATAGAAATG GGCTGCAAAG CTGTCCCATC
AAGGAAGACA GCTTCTTGCA GCGATACAGC TCAGACCCCA CAGGCGCCTT GACTGAGGAC
AGCATAGACG ACACCTTCCT CCCAGTGCCT GAATACATAA ACCAGTCCGT TCCCAAAAGG
CCCGCTGGCT CTGTGCAGAA TCCTGTCTAT CACAATCAGC CTCTGAACCC CGCGCCCAGC
AGAGACCCAC ACTACCAGGA CCCCCACAGC ACTGCAGTGG GCAACCCCGA GTATCTCAAC
ACTGTCCAGC CCACCTGTGT CAACAGCACA TTCGACAGCC CTGCCCACTG GGCCCAGAAA
GGCAGCCACC AAATTAGCCT GGACAACCCT GACTACCAGC AGGACTTCTT TCCCAAGGAA
GCCAAGCCAA ATGGCATCTT TAAGGGCTCC ACAGCTGAAA ATGCAGAATA CCTAAGGGTC
GCGCCACAAA GCAGTGAATT TATTGGAGCA TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005219.2
Translation

Target ORF information:

RefSeq Version NM_005228.3
Organism Homo sapiens(Human)
Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005228.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
CATCCAAACT GCACCTACGG ATGCACTGGG CCAGGTCTTG AAGGCTGTCC AACGAATGGG
CCTAAGATCC CGTCCATCGC CACTGGGATG GTGGGGGCCC TCCTCTTGCT GCTGGTGGTG
GCCCTGGGGA TCGGCCTCTT CATGCGAAGG CGCCACATCG TTCGGAAGCG CACGCTGCGG
AGGCTGCTGC AGGAGAGGGA GCTTGTGGAG CCTCTTACAC CCAGTGGAGA AGCTCCCAAC
CAAGCTCTCT TGAGGATCTT GAAGGAAACT GAATTCAAAA AGATCAAAGT GCTGGGCTCC
GGTGCGTTCG GCACGGTGTA TAAGGGACTC TGGATCCCAG AAGGTGAGAA AGTTAAAATT
CCCGTCGCTA TCAAGGAATT AAGAGAAGCA ACATCTCCGA AAGCCAACAA GGAAATCCTC
GATGAAGCCT ACGTGATGGC CAGCGTGGAC AACCCCCACG TGTGCCGCCT GCTGGGCATC
TGCCTCACCT CCACCGTGCA GCTCATCACG CAGCTCATGC CCTTCGGCTG CCTCCTGGAC
TATGTCCGGG AACACAAAGA CAATATTGGC TCCCAGTACC TGCTCAACTG GTGTGTGCAG
ATCGCAAAGG GCATGAACTA CTTGGAGGAC CGTCGCTTGG TGCACCGCGA CCTGGCAGCC
AGGAACGTAC TGGTGAAAAC ACCGCAGCAT GTCAAGATCA CAGATTTTGG GCTGGCCAAA
CTGCTGGGTG CGGAAGAGAA AGAATACCAT GCAGAAGGAG GCAAAGTGCC TATCAAGTGG
ATGGCATTGG AATCAATTTT ACACAGAATC TATACCCACC AGAGTGATGT CTGGAGCTAC
GGGGTGACCG TTTGGGAGTT GATGACCTTT GGATCCAAGC CATATGACGG AATCCCTGCC
AGCGAGATCT CCTCCATCCT GGAGAAAGGA GAACGCCTCC CTCAGCCACC CATATGTACC
ATCGATGTCT ACATGATCAT GGTCAAGTGC TGGATGATAG ACGCAGATAG TCGCCCAAAG
TTCCGTGAGT TGATCATCGA ATTCTCCAAA ATGGCCCGAG ACCCCCAGCG CTACCTTGTC
ATTCAGGGGG ATGAAAGAAT GCATTTGCCA AGTCCTACAG ACTCCAACTT CTACCGTGCC
CTGATGGATG AAGAAGACAT GGACGACGTG GTGGATGCCG ACGAGTACCT CATCCCACAG
CAGGGCTTCT TCAGCAGCCC CTCCACGTCA CGGACTCCCC TCCTGAGCTC TCTGAGTGCA
ACCAGCAACA ATTCCACCGT GGCTTGCATT GATAGAAATG GGCTGCAAAG CTGTCCCATC
AAGGAAGACA GCTTCTTGCA GCGATACAGC TCAGACCCCA CAGGCGCCTT GACTGAGGAC
AGCATAGACG ACACCTTCCT CCCAGTGCCT GAATACATAA ACCAGTCCGT TCCCAAAAGG
CCCGCTGGCT CTGTGCAGAA TCCTGTCTAT CACAATCAGC CTCTGAACCC CGCGCCCAGC
AGAGACCCAC ACTACCAGGA CCCCCACAGC ACTGCAGTGG GCAACCCCGA GTATCTCAAC
ACTGTCCAGC CCACCTGTGT CAACAGCACA TTCGACAGCC CTGCCCACTG GGCCCAGAAA
GGCAGCCACC AAATTAGCCT GGACAACCCT GACTACCAGC AGGACTTCTT TCCCAAGGAA
GCCAAGCCAA ATGGCATCTT TAAGGGCTCC ACAGCTGAAA ATGCAGAATA CCTAAGGGTC
GCGCCACAAA GCAGTGAATT TATTGGAGCA TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25398
Clone ID Related Accession (Same CDS sequence) NM_201284.1
Accession Version NM_201284.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2118bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product epidermal growth factor receptor isoform d precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AW163038.1, X00588.1 and AF125253.1. Transcript Variant: This variant (4) uses a different 3' terminal exon when compared to variant 1. The resulting isoform (d) has a shorter and distinct C-terminus. Only the extracellular domain is present in isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF125253.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149398, SAMN03465410 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
CATCCAAACT GCACCTACGG GCCAGGAAAT GAGAGTCTCA AAGCCATGTT ATTCTGCCTT
TTTAAACTAT CATCCTGTAA TCAAAGTAAT GATGGCAGCG TGTCCCACCA GAGCGGGAGC
CCAGCTGCTC AGGAGTCATG CTTAGGATGG ATCCCTTCTC TTCTGCCGTC AGAGTTTCAG
CTGGGTTGGG GTGGATGCAG CCACCTCCAT GCCTGGCCTT CTGCATCTGT GATCATCACG
GCCTCCTCCT GCCACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_958441.1
CDS247..2364
Misc Feature(1)208..210
Misc Feature(2)order(337..339,418..420)
Misc Feature(3)412..414
Misc Feature(4)415..750
Misc Feature(5)469..1146
Misc Feature(6)628..630
Misc Feature(7)order(715..717,805..807)
Misc Feature(8)769..771
Misc Feature(9)799..1251
Misc Feature(10)order(814..816,841..843)
Misc Feature(11)order(826..828,865..867)
Misc Feature(12)832..834
Misc Feature(13)order(889..891,913..915)
Misc Feature(14)order(901..903,937..939)
Misc Feature(15)931..933
Misc Feature(16)937..1068
Misc Feature(17)order(940..942,964..966)
Misc Feature(18)order(952..954,988..990)
Misc Feature(19)order(997..999,1024..1026)
Misc Feature(20)order(1036..1038,1117..1119)
Misc Feature(21)order(1129..1131,1165..1167)
Misc Feature(22)order(1177..1179,1222..1224)
Misc Feature(23)order(1231..1233,1243..1245)
Misc Feature(24)order(1255..1257,1330..1332)
Misc Feature(25)1300..1302
Misc Feature(26)1327..1689
Misc Feature(27)1327..1329
Misc Feature(28)1414..2046
Misc Feature(29)1483..1485
Misc Feature(30)1576..1578
Misc Feature(31)order(1654..1656,1741..1743)
Misc Feature(32)1759..2127
Misc Feature(33)1762..1923
Misc Feature(34)order(1762..1764,1789..1791)
Misc Feature(35)order(1774..1776,1813..1815)
Misc Feature(36)order(1822..1824,1849..1851)
Misc Feature(37)1828..1830
Misc Feature(38)order(1861..1863,1909..1911)
Misc Feature(39)1900..2040
Misc Feature(40)order(1918..1920,1957..1959)
Misc Feature(41)order(1930..1932,1981..1983)
Misc Feature(42)1948..1950
Misc Feature(43)order(1990..1992,2017..2019)
Misc Feature(44)order(2029..2031,2095..2097)
Misc Feature(45)2053..2055
Misc Feature(46)2113..2115
Misc Feature(47)2323..2325
Exon (1)1..334
Exon (2)335..486
Exon (3)487..670
Exon (4)671..805
Exon (5)806..874
Exon (6)875..993
Exon (7)994..1135
Exon (8)1136..1252
Exon (9)1253..1379
Exon (10)1380..1453
Exon (11)1454..1544
Exon (12)1545..1744
Exon (13)1745..1877
Exon (14)1878..1968
Exon (15)1969..2126
Exon (16)2127..2865
Translation

Target ORF information:

RefSeq Version NM_201284.1
Organism Homo sapiens(Human)
Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_201284.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
CATCCAAACT GCACCTACGG GCCAGGAAAT GAGAGTCTCA AAGCCATGTT ATTCTGCCTT
TTTAAACTAT CATCCTGTAA TCAAAGTAAT GATGGCAGCG TGTCCCACCA GAGCGGGAGC
CCAGCTGCTC AGGAGTCATG CTTAGGATGG ATCCCTTCTC TTCTGCCGTC AGAGTTTCAG
CTGGGTTGGG GTGGATGCAG CCACCTCCAT GCCTGGCCTT CTGCATCTGT GATCATCACG
GCCTCCTCCT GCCACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu102763
Clone ID Related Accession (Same CDS sequence) NM_001346941.1
Accession Version NM_001346941.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2832bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product epidermal growth factor receptor isoform i precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006977.3 and AC073324.6. Transcript Variant: This variant (EGFRvIII, also known as delta-EGFR and de2-7EGFR) has an in-frame deletion of six exons in the 5' coding region, compared to variant 1. The encoded isoform (i) has a shorter extracellular domain compared to isoform a. This variant is considered to be tumorigenic and the encoded protein lacks normal ligand binding ability and is constitutively active (PMID: 23777544). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## inferred exon combination :: PMID: 23777544 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGGT AATTATGTGG TGACAGATCA CGGCTCGTGC
GTCCGAGCCT GTGGGGCCGA CAGCTATGAG ATGGAGGAAG ACGGCGTCCG CAAGTGTAAG
AAGTGCGAAG GGCCTTGCCG CAAAGTGTGT AACGGAATAG GTATTGGTGA ATTTAAAGAC
TCACTCTCCA TAAATGCTAC GAATATTAAA CACTTCAAAA ACTGCACCTC CATCAGTGGC
GATCTCCACA TCCTGCCGGT GGCATTTAGG GGTGACTCCT TCACACATAC TCCTCCTCTG
GATCCACAGG AACTGGATAT TCTGAAAACC GTAAAGGAAA TCACAGGGTT TTTGCTGATT
CAGGCTTGGC CTGAAAACAG GACGGACCTC CATGCCTTTG AGAACCTAGA AATCATACGC
GGCAGGACCA AGCAACATGG TCAGTTTTCT CTTGCAGTCG TCAGCCTGAA CATAACATCC
TTGGGATTAC GCTCCCTCAA GGAGATAAGT GATGGAGATG TGATAATTTC AGGAAACAAA
AATTTGTGCT ATGCAAATAC AATAAACTGG AAAAAACTGT TTGGGACCTC CGGTCAGAAA
ACCAAAATTA TAAGCAACAG AGGTGAAAAC AGCTGCAAGG CCACAGGCCA GGTCTGCCAT
GCCTTGTGCT CCCCCGAGGG CTGCTGGGGC CCGGAGCCCA GGGACTGCGT CTCTTGCCGG
AATGTCAGCC GAGGCAGGGA ATGCGTGGAC AAGTGCAACC TTCTGGAGGG TGAGCCAAGG
GAGTTTGTGG AGAACTCTGA GTGCATACAG TGCCACCCAG AGTGCCTGCC TCAGGCCATG
AACATCACCT GCACAGGACG GGGACCAGAC AACTGTATCC AGTGTGCCCA CTACATTGAC
GGCCCCCACT GCGTCAAGAC CTGCCCGGCA GGAGTCATGG GAGAAAACAA CACCCTGGTC
TGGAAGTACG CAGACGCCGG CCATGTGTGC CACCTGTGCC ATCCAAACTG CACCTACGGA
TGCACTGGGC CAGGTCTTGA AGGCTGTCCA ACGAATGGGC CTAAGATCCC GTCCATCGCC
ACTGGGATGG TGGGGGCCCT CCTCTTGCTG CTGGTGGTGG CCCTGGGGAT CGGCCTCTTC
ATGCGAAGGC GCCACATCGT TCGGAAGCGC ACGCTGCGGA GGCTGCTGCA GGAGAGGGAG
CTTGTGGAGC CTCTTACACC CAGTGGAGAA GCTCCCAACC AAGCTCTCTT GAGGATCTTG
AAGGAAACTG AATTCAAAAA GATCAAAGTG CTGGGCTCCG GTGCGTTCGG CACGGTGTAT
AAGGGACTCT GGATCCCAGA AGGTGAGAAA GTTAAAATTC CCGTCGCTAT CAAGGAATTA
AGAGAAGCAA CATCTCCGAA AGCCAACAAG GAAATCCTCG ATGAAGCCTA CGTGATGGCC
AGCGTGGACA ACCCCCACGT GTGCCGCCTG CTGGGCATCT GCCTCACCTC CACCGTGCAG
CTCATCACGC AGCTCATGCC CTTCGGCTGC CTCCTGGACT ATGTCCGGGA ACACAAAGAC
AATATTGGCT CCCAGTACCT GCTCAACTGG TGTGTGCAGA TCGCAAAGGG CATGAACTAC
TTGGAGGACC GTCGCTTGGT GCACCGCGAC CTGGCAGCCA GGAACGTACT GGTGAAAACA
CCGCAGCATG TCAAGATCAC AGATTTTGGG CTGGCCAAAC TGCTGGGTGC GGAAGAGAAA
GAATACCATG CAGAAGGAGG CAAAGTGCCT ATCAAGTGGA TGGCATTGGA ATCAATTTTA
CACAGAATCT ATACCCACCA GAGTGATGTC TGGAGCTACG GGGTGACTGT TTGGGAGTTG
ATGACCTTTG GATCCAAGCC ATATGACGGA ATCCCTGCCA GCGAGATCTC CTCCATCCTG
GAGAAAGGAG AACGCCTCCC TCAGCCACCC ATATGTACCA TCGATGTCTA CATGATCATG
GTCAAGTGCT GGATGATAGA CGCAGATAGT CGCCCAAAGT TCCGTGAGTT GATCATCGAA
TTCTCCAAAA TGGCCCGAGA CCCCCAGCGC TACCTTGTCA TTCAGGGGGA TGAAAGAATG
CATTTGCCAA GTCCTACAGA CTCCAACTTC TACCGTGCCC TGATGGATGA AGAAGACATG
GACGACGTGG TGGATGCCGA CGAGTACCTC ATCCCACAGC AGGGCTTCTT CAGCAGCCCC
TCCACGTCAC GGACTCCCCT CCTGAGCTCT CTGAGTGCAA CCAGCAACAA TTCCACCGTG
GCTTGCATTG ATAGAAATGG GCTGCAAAGC TGTCCCATCA AGGAAGACAG CTTCTTGCAG
CGATACAGCT CAGACCCCAC AGGCGCCTTG ACTGAGGACA GCATAGACGA CACCTTCCTC
CCAGTGCCTG AATACATAAA CCAGTCCGTT CCCAAAAGGC CCGCTGGCTC TGTGCAGAAT
CCTGTCTATC ACAATCAGCC TCTGAACCCC GCGCCCAGCA GAGACCCACA CTACCAGGAC
CCCCACAGCA CTGCAGTGGG CAACCCCGAG TATCTCAACA CTGTCCAGCC CACCTGTGTC
AACAGCACAT TCGACAGCCC TGCCCACTGG GCCCAGAAAG GCAGCCACCA AATTAGCCTG
GACAACCCTG ACTACCAGCA GGACTTCTTT CCCAAGGAAG CCAAGCCAAA TGGCATCTTT
AAGGGCTCCA CAGCTGAAAA TGCAGAATAC CTAAGGGTCG CGCCACAAAG CAGTGAATTT
ATTGGAGCAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001333870.1
CDS258..3089
Misc Feature(1)219..221
Exon (1)1..345
Exon (2)346..462
Exon (3)463..589
Exon (4)590..663
Exon (5)664..754
Exon (6)755..954
Exon (7)955..1087
Exon (8)1088..1178
Exon (9)1179..1336
Exon (10)1337..1375
Exon (11)1376..1517
Exon (12)1518..1640
Exon (13)1641..1739
Exon (14)1740..1925
Exon (15)1926..2081
Exon (16)2082..2157
Exon (17)2158..2304
Exon (18)2305..2402
Exon (19)2403..2570
Exon (20)2571..2618
Exon (21)2619..2727
Exon (22)2728..5552
Translation

Target ORF information:

RefSeq Version NM_001346941.1
Organism Homo sapiens(Human)
Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant EGFRvIII, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001346941.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGGT AATTATGTGG TGACAGATCA CGGCTCGTGC
GTCCGAGCCT GTGGGGCCGA CAGCTATGAG ATGGAGGAAG ACGGCGTCCG CAAGTGTAAG
AAGTGCGAAG GGCCTTGCCG CAAAGTGTGT AACGGAATAG GTATTGGTGA ATTTAAAGAC
TCACTCTCCA TAAATGCTAC GAATATTAAA CACTTCAAAA ACTGCACCTC CATCAGTGGC
GATCTCCACA TCCTGCCGGT GGCATTTAGG GGTGACTCCT TCACACATAC TCCTCCTCTG
GATCCACAGG AACTGGATAT TCTGAAAACC GTAAAGGAAA TCACAGGGTT TTTGCTGATT
CAGGCTTGGC CTGAAAACAG GACGGACCTC CATGCCTTTG AGAACCTAGA AATCATACGC
GGCAGGACCA AGCAACATGG TCAGTTTTCT CTTGCAGTCG TCAGCCTGAA CATAACATCC
TTGGGATTAC GCTCCCTCAA GGAGATAAGT GATGGAGATG TGATAATTTC AGGAAACAAA
AATTTGTGCT ATGCAAATAC AATAAACTGG AAAAAACTGT TTGGGACCTC CGGTCAGAAA
ACCAAAATTA TAAGCAACAG AGGTGAAAAC AGCTGCAAGG CCACAGGCCA GGTCTGCCAT
GCCTTGTGCT CCCCCGAGGG CTGCTGGGGC CCGGAGCCCA GGGACTGCGT CTCTTGCCGG
AATGTCAGCC GAGGCAGGGA ATGCGTGGAC AAGTGCAACC TTCTGGAGGG TGAGCCAAGG
GAGTTTGTGG AGAACTCTGA GTGCATACAG TGCCACCCAG AGTGCCTGCC TCAGGCCATG
AACATCACCT GCACAGGACG GGGACCAGAC AACTGTATCC AGTGTGCCCA CTACATTGAC
GGCCCCCACT GCGTCAAGAC CTGCCCGGCA GGAGTCATGG GAGAAAACAA CACCCTGGTC
TGGAAGTACG CAGACGCCGG CCATGTGTGC CACCTGTGCC ATCCAAACTG CACCTACGGA
TGCACTGGGC CAGGTCTTGA AGGCTGTCCA ACGAATGGGC CTAAGATCCC GTCCATCGCC
ACTGGGATGG TGGGGGCCCT CCTCTTGCTG CTGGTGGTGG CCCTGGGGAT CGGCCTCTTC
ATGCGAAGGC GCCACATCGT TCGGAAGCGC ACGCTGCGGA GGCTGCTGCA GGAGAGGGAG
CTTGTGGAGC CTCTTACACC CAGTGGAGAA GCTCCCAACC AAGCTCTCTT GAGGATCTTG
AAGGAAACTG AATTCAAAAA GATCAAAGTG CTGGGCTCCG GTGCGTTCGG CACGGTGTAT
AAGGGACTCT GGATCCCAGA AGGTGAGAAA GTTAAAATTC CCGTCGCTAT CAAGGAATTA
AGAGAAGCAA CATCTCCGAA AGCCAACAAG GAAATCCTCG ATGAAGCCTA CGTGATGGCC
AGCGTGGACA ACCCCCACGT GTGCCGCCTG CTGGGCATCT GCCTCACCTC CACCGTGCAG
CTCATCACGC AGCTCATGCC CTTCGGCTGC CTCCTGGACT ATGTCCGGGA ACACAAAGAC
AATATTGGCT CCCAGTACCT GCTCAACTGG TGTGTGCAGA TCGCAAAGGG CATGAACTAC
TTGGAGGACC GTCGCTTGGT GCACCGCGAC CTGGCAGCCA GGAACGTACT GGTGAAAACA
CCGCAGCATG TCAAGATCAC AGATTTTGGG CTGGCCAAAC TGCTGGGTGC GGAAGAGAAA
GAATACCATG CAGAAGGAGG CAAAGTGCCT ATCAAGTGGA TGGCATTGGA ATCAATTTTA
CACAGAATCT ATACCCACCA GAGTGATGTC TGGAGCTACG GGGTGACTGT TTGGGAGTTG
ATGACCTTTG GATCCAAGCC ATATGACGGA ATCCCTGCCA GCGAGATCTC CTCCATCCTG
GAGAAAGGAG AACGCCTCCC TCAGCCACCC ATATGTACCA TCGATGTCTA CATGATCATG
GTCAAGTGCT GGATGATAGA CGCAGATAGT CGCCCAAAGT TCCGTGAGTT GATCATCGAA
TTCTCCAAAA TGGCCCGAGA CCCCCAGCGC TACCTTGTCA TTCAGGGGGA TGAAAGAATG
CATTTGCCAA GTCCTACAGA CTCCAACTTC TACCGTGCCC TGATGGATGA AGAAGACATG
GACGACGTGG TGGATGCCGA CGAGTACCTC ATCCCACAGC AGGGCTTCTT CAGCAGCCCC
TCCACGTCAC GGACTCCCCT CCTGAGCTCT CTGAGTGCAA CCAGCAACAA TTCCACCGTG
GCTTGCATTG ATAGAAATGG GCTGCAAAGC TGTCCCATCA AGGAAGACAG CTTCTTGCAG
CGATACAGCT CAGACCCCAC AGGCGCCTTG ACTGAGGACA GCATAGACGA CACCTTCCTC
CCAGTGCCTG AATACATAAA CCAGTCCGTT CCCAAAAGGC CCGCTGGCTC TGTGCAGAAT
CCTGTCTATC ACAATCAGCC TCTGAACCCC GCGCCCAGCA GAGACCCACA CTACCAGGAC
CCCCACAGCA CTGCAGTGGG CAACCCCGAG TATCTCAACA CTGTCCAGCC CACCTGTGTC
AACAGCACAT TCGACAGCCC TGCCCACTGG GCCCAGAAAG GCAGCCACCA AATTAGCCTG
GACAACCCTG ACTACCAGCA GGACTTCTTT CCCAAGGAAG CCAAGCCAAA TGGCATCTTT
AAGGGCTCCA CAGCTGAAAA TGCAGAATAC CTAAGGGTCG CGCCACAAAG CAGTGAATTT
ATTGGAGCAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu102753
Clone ID Related Accession (Same CDS sequence) NM_001346900.1
Accession Version NM_001346900.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product epidermal growth factor receptor isoform h
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC347326.1, AK294750.1, AC006977.3, AC073324.6, HQ912715.1, DA371223.1 and AW450657.1. Transcript Variant: This variant (8) uses a novel 5' terminal exon compared to variant 1. The encoded isoform (h) has a shorter and distinct N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK294750.1, SRR1803613.174821.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGTTCAATA ACTGTGAGGT GGTCCTTGGG AATTTGGAAA TTACCTATGT GCAGAGGAAT 
TATGATCTTT CCTTCTTAAA GACCATCCAG GAGGTGGCTG GTTATGTCCT CATTGCCCTC
AACACAGTGG AGCGAATTCC TTTGGAAAAC CTGCAGATCA TCAGAGGAAA TATGTACTAC
GAAAATTCCT ATGCCTTAGC AGTCTTATCT AACTATGATG CAAATAAAAC CGGACTGAAG
GAGCTGCCCA TGAGAAATTT ACAGGAAATC CTGCATGGCG CCGTGCGGTT CAGCAACAAC
CCTGCCCTGT GCAACGTGGA GAGCATCCAG TGGCGGGACA TAGTCAGCAG TGACTTTCTC
AGCAACATGT CGATGGACTT CCAGAACCAC CTGGGCAGCT GCCAAAAGTG TGATCCAAGC
TGTCCCAATG GGAGCTGCTG GGGTGCAGGA GAGGAGAACT GCCAGAAACT GACCAAAATC
ATCTGTGCCC AGCAGTGCTC CGGGCGCTGC CGTGGCAAGT CCCCCAGTGA CTGCTGCCAC
AACCAGTGTG CTGCAGGCTG CACAGGCCCC CGGGAGAGCG ACTGCCTGGT CTGCCGCAAA
TTCCGAGACG AAGCCACGTG CAAGGACACC TGCCCCCCAC TCATGCTCTA CAACCCCACC
ACGTACCAGA TGGATGTGAA CCCCGAGGGC AAATACAGCT TTGGTGCCAC CTGCGTGAAG
AAGTGTCCCC GTAATTATGT GGTGACAGAT CACGGCTCGT GCGTCCGAGC CTGTGGGGCC
GACAGCTATG AGATGGAGGA AGACGGCGTC CGCAAGTGTA AGAAGTGCGA AGGGCCTTGC
CGCAAAGTGT GTAACGGAAT AGGTATTGGT GAATTTAAAG ACTCACTCTC CATAAATGCT
ACGAATATTA AACACTTCAA AAACTGCACC TCCATCAGTG GCGATCTCCA CATCCTGCCG
GTGGCATTTA GGGGTGACTC CTTCACACAT ACTCCTCCTC TGGATCCACA GGAACTGGAT
ATTCTGAAAA CCGTAAAGGA AATCACAGGG TTTTTGCTGA TTCAGGCTTG GCCTGAAAAC
AGGACGGACC TCCATGCCTT TGAGAACCTA GAAATCATAC GCGGCAGGAC CAAGCAACAT
GGTCAGTTTT CTCTTGCAGT CGTCAGCCTG AACATAACAT CCTTGGGATT ACGCTCCCTC
AAGGAGATAA GTGATGGAGA TGTGATAATT TCAGGAAACA AAAATTTGTG CTATGCAAAT
ACAATAAACT GGAAAAAACT GTTTGGGACC TCCGGTCAGA AAACCAAAAT TATAAGCAAC
AGAGGTGAAA ACAGCTGCAA GGCCACAGGC CAGGTCTGCC ATGCCTTGTG CTCCCCCGAG
GGCTGCTGGG GCCCGGAGCC CAGGGACTGC GTCTCTTGCC GGAATGTCAG CCGAGGCAGG
GAATGCGTGG ACAAGTGCAA CCTTCTGGAG GGTGAGCCAA GGGAGTTTGT GGAGAACTCT
GAGTGCATAC AGTGCCACCC AGAGTGCCTG CCTCAGGCCA TGAACATCAC CTGCACAGGA
CGGGGACCAG ACAACTGTAT CCAGTGTGCC CACTACATTG ACGGCCCCCA CTGCGTCAAG
ACCTGCCCGG CAGGAGTCAT GGGAGAAAAC AACACCCTGG TCTGGAAGTA CGCAGACGCC
GGCCATGTGT GCCACCTGTG CCATCCAAAC TGCACCTACG GATGCACTGG GCCAGGTCTT
GAAGGCTGTC CAACGAATGG GCCTAAGATC CCGTCCATCG CCACTGGGAT GGTGGGGGCC
CTCCTCTTGC TGCTGGTGGT GGCCCTGGGG ATCGGCCTCT TCATGCGAAG GCGCCACATC
GTTCGGAAGC GCACGCTGCG GAGGCTGCTG CAGGAGAGGG AGCTTGTGGA GCCTCTTACA
CCCAGTGGAG AAGCTCCCAA CCAAGCTCTC TTGAGGATCT TGAAGGAAAC TGAATTCAAA
AAGATCAAAG TGCTGGGCTC CGGTGCGTTC GGCACGGTGT ATAAGGGACT CTGGATCCCA
GAAGGTGAGA AAGTTAAAAT TCCCGTCGCT ATCAAGGAAT TAAGAGAAGC AACATCTCCG
AAAGCCAACA AGGAAATCCT CGATGAAGCC TACGTGATGG CCAGCGTGGA CAACCCCCAC
GTGTGCCGCC TGCTGGGCAT CTGCCTCACC TCCACCGTGC AGCTCATCAC GCAGCTCATG
CCCTTCGGCT GCCTCCTGGA CTATGTCCGG GAACACAAAG ACAATATTGG CTCCCAGTAC
CTGCTCAACT GGTGTGTGCA GATCGCAAAG GGCATGAACT ACTTGGAGGA CCGTCGCTTG
GTGCACCGCG ACCTGGCAGC CAGGAACGTA CTGGTGAAAA CACCGCAGCA TGTCAAGATC
ACAGATTTTG GGCTGGCCAA ACTGCTGGGT GCGGAAGAGA AAGAATACCA TGCAGAAGGA
GGCAAAGTGC CTATCAAGTG GATGGCATTG GAATCAATTT TACACAGAAT CTATACCCAC
CAGAGTGATG TCTGGAGCTA CGGGGTGACT GTTTGGGAGT TGATGACCTT TGGATCCAAG
CCATATGACG GAATCCCTGC CAGCGAGATC TCCTCCATCC TGGAGAAAGG AGAACGCCTC
CCTCAGCCAC CCATATGTAC CATCGATGTC TACATGATCA TGGTCAAGTG CTGGATGATA
GACGCAGATA GTCGCCCAAA GTTCCGTGAG TTGATCATCG AATTCTCCAA AATGGCCCGA
GACCCCCAGC GCTACCTTGT CATTCAGGGG GATGAAAGAA TGCATTTGCC AAGTCCTACA
GACTCCAACT TCTACCGTGC CCTGATGGAT GAAGAAGACA TGGACGACGT GGTGGATGCC
GACGAGTACC TCATCCCACA GCAGGGCTTC TTCAGCAGCC CCTCCACGTC ACGGACTCCC
CTCCTGAGCT CTCTGAGTGC AACCAGCAAC AATTCCACCG TGGCTTGCAT TGATAGAAAT
GGGCTGCAAA GCTGTCCCAT CAAGGAAGAC AGCTTCTTGC AGCGATACAG CTCAGACCCC
ACAGGCGCCT TGACTGAGGA CAGCATAGAC GACACCTTCC TCCCAGTGCC TGAATACATA
AACCAGTCCG TTCCCAAAAG GCCCGCTGGC TCTGTGCAGA ATCCTGTCTA TCACAATCAG
CCTCTGAACC CCGCGCCCAG CAGAGACCCA CACTACCAGG ACCCCCACAG CACTGCAGTG
GGCAACCCCG AGTATCTCAA CACTGTCCAG CCCACCTGTG TCAACAGCAC ATTCGACAGC
CCTGCCCACT GGGCCCAGAA AGGCAGCCAC CAAATTAGCC TGGACAACCC TGACTACCAG
CAGGACTTCT TTCCCAAGGA AGCCAAGCCA AATGGCATCT TTAAGGGCTC CACAGCTGAA
AATGCAGAAT ACCTAAGGGT CGCGCCACAA AGCAGTGAAT TTATTGGAGC ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001333829.1
CDS214..3687
Misc Feature(1)136..138
Exon (1)1..142
Exon (2)143..294
Exon (3)295..478
Exon (4)479..613
Exon (5)614..682
Exon (6)683..801
Exon (7)802..943
Exon (8)944..1060
Exon (9)1061..1187
Exon (10)1188..1261
Exon (11)1262..1352
Exon (12)1353..1552
Exon (13)1553..1685
Exon (14)1686..1776
Exon (15)1777..1934
Exon (16)1935..1973
Exon (17)1974..2115
Exon (18)2116..2238
Exon (19)2239..2337
Exon (20)2338..2523
Exon (21)2524..2679
Exon (22)2680..2755
Exon (23)2756..2902
Exon (24)2903..3000
Exon (25)3001..3168
Exon (26)3169..3216
Exon (27)3217..3325
Exon (28)3326..6150
Translation

Target ORF information:

RefSeq Version NM_001346900.1
Organism Homo sapiens(Human)
Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001346900.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGTTCAATA ACTGTGAGGT GGTCCTTGGG AATTTGGAAA TTACCTATGT GCAGAGGAAT 
TATGATCTTT CCTTCTTAAA GACCATCCAG GAGGTGGCTG GTTATGTCCT CATTGCCCTC
AACACAGTGG AGCGAATTCC TTTGGAAAAC CTGCAGATCA TCAGAGGAAA TATGTACTAC
GAAAATTCCT ATGCCTTAGC AGTCTTATCT AACTATGATG CAAATAAAAC CGGACTGAAG
GAGCTGCCCA TGAGAAATTT ACAGGAAATC CTGCATGGCG CCGTGCGGTT CAGCAACAAC
CCTGCCCTGT GCAACGTGGA GAGCATCCAG TGGCGGGACA TAGTCAGCAG TGACTTTCTC
AGCAACATGT CGATGGACTT CCAGAACCAC CTGGGCAGCT GCCAAAAGTG TGATCCAAGC
TGTCCCAATG GGAGCTGCTG GGGTGCAGGA GAGGAGAACT GCCAGAAACT GACCAAAATC
ATCTGTGCCC AGCAGTGCTC CGGGCGCTGC CGTGGCAAGT CCCCCAGTGA CTGCTGCCAC
AACCAGTGTG CTGCAGGCTG CACAGGCCCC CGGGAGAGCG ACTGCCTGGT CTGCCGCAAA
TTCCGAGACG AAGCCACGTG CAAGGACACC TGCCCCCCAC TCATGCTCTA CAACCCCACC
ACGTACCAGA TGGATGTGAA CCCCGAGGGC AAATACAGCT TTGGTGCCAC CTGCGTGAAG
AAGTGTCCCC GTAATTATGT GGTGACAGAT CACGGCTCGT GCGTCCGAGC CTGTGGGGCC
GACAGCTATG AGATGGAGGA AGACGGCGTC CGCAAGTGTA AGAAGTGCGA AGGGCCTTGC
CGCAAAGTGT GTAACGGAAT AGGTATTGGT GAATTTAAAG ACTCACTCTC CATAAATGCT
ACGAATATTA AACACTTCAA AAACTGCACC TCCATCAGTG GCGATCTCCA CATCCTGCCG
GTGGCATTTA GGGGTGACTC CTTCACACAT ACTCCTCCTC TGGATCCACA GGAACTGGAT
ATTCTGAAAA CCGTAAAGGA AATCACAGGG TTTTTGCTGA TTCAGGCTTG GCCTGAAAAC
AGGACGGACC TCCATGCCTT TGAGAACCTA GAAATCATAC GCGGCAGGAC CAAGCAACAT
GGTCAGTTTT CTCTTGCAGT CGTCAGCCTG AACATAACAT CCTTGGGATT ACGCTCCCTC
AAGGAGATAA GTGATGGAGA TGTGATAATT TCAGGAAACA AAAATTTGTG CTATGCAAAT
ACAATAAACT GGAAAAAACT GTTTGGGACC TCCGGTCAGA AAACCAAAAT TATAAGCAAC
AGAGGTGAAA ACAGCTGCAA GGCCACAGGC CAGGTCTGCC ATGCCTTGTG CTCCCCCGAG
GGCTGCTGGG GCCCGGAGCC CAGGGACTGC GTCTCTTGCC GGAATGTCAG CCGAGGCAGG
GAATGCGTGG ACAAGTGCAA CCTTCTGGAG GGTGAGCCAA GGGAGTTTGT GGAGAACTCT
GAGTGCATAC AGTGCCACCC AGAGTGCCTG CCTCAGGCCA TGAACATCAC CTGCACAGGA
CGGGGACCAG ACAACTGTAT CCAGTGTGCC CACTACATTG ACGGCCCCCA CTGCGTCAAG
ACCTGCCCGG CAGGAGTCAT GGGAGAAAAC AACACCCTGG TCTGGAAGTA CGCAGACGCC
GGCCATGTGT GCCACCTGTG CCATCCAAAC TGCACCTACG GATGCACTGG GCCAGGTCTT
GAAGGCTGTC CAACGAATGG GCCTAAGATC CCGTCCATCG CCACTGGGAT GGTGGGGGCC
CTCCTCTTGC TGCTGGTGGT GGCCCTGGGG ATCGGCCTCT TCATGCGAAG GCGCCACATC
GTTCGGAAGC GCACGCTGCG GAGGCTGCTG CAGGAGAGGG AGCTTGTGGA GCCTCTTACA
CCCAGTGGAG AAGCTCCCAA CCAAGCTCTC TTGAGGATCT TGAAGGAAAC TGAATTCAAA
AAGATCAAAG TGCTGGGCTC CGGTGCGTTC GGCACGGTGT ATAAGGGACT CTGGATCCCA
GAAGGTGAGA AAGTTAAAAT TCCCGTCGCT ATCAAGGAAT TAAGAGAAGC AACATCTCCG
AAAGCCAACA AGGAAATCCT CGATGAAGCC TACGTGATGG CCAGCGTGGA CAACCCCCAC
GTGTGCCGCC TGCTGGGCAT CTGCCTCACC TCCACCGTGC AGCTCATCAC GCAGCTCATG
CCCTTCGGCT GCCTCCTGGA CTATGTCCGG GAACACAAAG ACAATATTGG CTCCCAGTAC
CTGCTCAACT GGTGTGTGCA GATCGCAAAG GGCATGAACT ACTTGGAGGA CCGTCGCTTG
GTGCACCGCG ACCTGGCAGC CAGGAACGTA CTGGTGAAAA CACCGCAGCA TGTCAAGATC
ACAGATTTTG GGCTGGCCAA ACTGCTGGGT GCGGAAGAGA AAGAATACCA TGCAGAAGGA
GGCAAAGTGC CTATCAAGTG GATGGCATTG GAATCAATTT TACACAGAAT CTATACCCAC
CAGAGTGATG TCTGGAGCTA CGGGGTGACT GTTTGGGAGT TGATGACCTT TGGATCCAAG
CCATATGACG GAATCCCTGC CAGCGAGATC TCCTCCATCC TGGAGAAAGG AGAACGCCTC
CCTCAGCCAC CCATATGTAC CATCGATGTC TACATGATCA TGGTCAAGTG CTGGATGATA
GACGCAGATA GTCGCCCAAA GTTCCGTGAG TTGATCATCG AATTCTCCAA AATGGCCCGA
GACCCCCAGC GCTACCTTGT CATTCAGGGG GATGAAAGAA TGCATTTGCC AAGTCCTACA
GACTCCAACT TCTACCGTGC CCTGATGGAT GAAGAAGACA TGGACGACGT GGTGGATGCC
GACGAGTACC TCATCCCACA GCAGGGCTTC TTCAGCAGCC CCTCCACGTC ACGGACTCCC
CTCCTGAGCT CTCTGAGTGC AACCAGCAAC AATTCCACCG TGGCTTGCAT TGATAGAAAT
GGGCTGCAAA GCTGTCCCAT CAAGGAAGAC AGCTTCTTGC AGCGATACAG CTCAGACCCC
ACAGGCGCCT TGACTGAGGA CAGCATAGAC GACACCTTCC TCCCAGTGCC TGAATACATA
AACCAGTCCG TTCCCAAAAG GCCCGCTGGC TCTGTGCAGA ATCCTGTCTA TCACAATCAG
CCTCTGAACC CCGCGCCCAG CAGAGACCCA CACTACCAGG ACCCCCACAG CACTGCAGTG
GGCAACCCCG AGTATCTCAA CACTGTCCAG CCCACCTGTG TCAACAGCAC ATTCGACAGC
CCTGCCCACT GGGCCCAGAA AGGCAGCCAC CAAATTAGCC TGGACAACCC TGACTACCAG
CAGGACTTCT TTCCCAAGGA AGCCAAGCCA AATGGCATCT TTAAGGGCTC CACAGCTGAA
AATGCAGAAT ACCTAAGGGT CGCGCCACAA AGCAGTGAAT TTATTGGAGC ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu102757
Clone ID Related Accession (Same CDS sequence) NM_005228.4
Accession Version NM_005228.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product epidermal growth factor receptor isoform a precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC094761.1, HQ912715.1, X00588.1, AF125253.1, AU137334.1, AC073324.6, DA371223.1 and AW450657.1. On Nov 9, 2016 this sequence version replaced NM_005228.3. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X00588.1, SRR1803616.241434.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
CATCCAAACT GCACCTACGG ATGCACTGGG CCAGGTCTTG AAGGCTGTCC AACGAATGGG
CCTAAGATCC CGTCCATCGC CACTGGGATG GTGGGGGCCC TCCTCTTGCT GCTGGTGGTG
GCCCTGGGGA TCGGCCTCTT CATGCGAAGG CGCCACATCG TTCGGAAGCG CACGCTGCGG
AGGCTGCTGC AGGAGAGGGA GCTTGTGGAG CCTCTTACAC CCAGTGGAGA AGCTCCCAAC
CAAGCTCTCT TGAGGATCTT GAAGGAAACT GAATTCAAAA AGATCAAAGT GCTGGGCTCC
GGTGCGTTCG GCACGGTGTA TAAGGGACTC TGGATCCCAG AAGGTGAGAA AGTTAAAATT
CCCGTCGCTA TCAAGGAATT AAGAGAAGCA ACATCTCCGA AAGCCAACAA GGAAATCCTC
GATGAAGCCT ACGTGATGGC CAGCGTGGAC AACCCCCACG TGTGCCGCCT GCTGGGCATC
TGCCTCACCT CCACCGTGCA GCTCATCACG CAGCTCATGC CCTTCGGCTG CCTCCTGGAC
TATGTCCGGG AACACAAAGA CAATATTGGC TCCCAGTACC TGCTCAACTG GTGTGTGCAG
ATCGCAAAGG GCATGAACTA CTTGGAGGAC CGTCGCTTGG TGCACCGCGA CCTGGCAGCC
AGGAACGTAC TGGTGAAAAC ACCGCAGCAT GTCAAGATCA CAGATTTTGG GCTGGCCAAA
CTGCTGGGTG CGGAAGAGAA AGAATACCAT GCAGAAGGAG GCAAAGTGCC TATCAAGTGG
ATGGCATTGG AATCAATTTT ACACAGAATC TATACCCACC AGAGTGATGT CTGGAGCTAC
GGGGTGACTG TTTGGGAGTT GATGACCTTT GGATCCAAGC CATATGACGG AATCCCTGCC
AGCGAGATCT CCTCCATCCT GGAGAAAGGA GAACGCCTCC CTCAGCCACC CATATGTACC
ATCGATGTCT ACATGATCAT GGTCAAGTGC TGGATGATAG ACGCAGATAG TCGCCCAAAG
TTCCGTGAGT TGATCATCGA ATTCTCCAAA ATGGCCCGAG ACCCCCAGCG CTACCTTGTC
ATTCAGGGGG ATGAAAGAAT GCATTTGCCA AGTCCTACAG ACTCCAACTT CTACCGTGCC
CTGATGGATG AAGAAGACAT GGACGACGTG GTGGATGCCG ACGAGTACCT CATCCCACAG
CAGGGCTTCT TCAGCAGCCC CTCCACGTCA CGGACTCCCC TCCTGAGCTC TCTGAGTGCA
ACCAGCAACA ATTCCACCGT GGCTTGCATT GATAGAAATG GGCTGCAAAG CTGTCCCATC
AAGGAAGACA GCTTCTTGCA GCGATACAGC TCAGACCCCA CAGGCGCCTT GACTGAGGAC
AGCATAGACG ACACCTTCCT CCCAGTGCCT GAATACATAA ACCAGTCCGT TCCCAAAAGG
CCCGCTGGCT CTGTGCAGAA TCCTGTCTAT CACAATCAGC CTCTGAACCC CGCGCCCAGC
AGAGACCCAC ACTACCAGGA CCCCCACAGC ACTGCAGTGG GCAACCCCGA GTATCTCAAC
ACTGTCCAGC CCACCTGTGT CAACAGCACA TTCGACAGCC CTGCCCACTG GGCCCAGAAA
GGCAGCCACC AAATTAGCCT GGACAACCCT GACTACCAGC AGGACTTCTT TCCCAAGGAA
GCCAAGCCAA ATGGCATCTT TAAGGGCTCC ACAGCTGAAA ATGCAGAATA CCTAAGGGTC
GCGCCACAAA GCAGTGAATT TATTGGAGCA TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005219.2
CDS258..3890
Misc Feature(1)219..221
Misc Feature(2)order(348..350,429..431)
Misc Feature(3)423..425
Misc Feature(4)426..761
Misc Feature(5)480..1157
Misc Feature(6)639..641
Misc Feature(7)order(726..728,816..818)
Misc Feature(8)780..782
Misc Feature(9)810..1262
Misc Feature(10)order(825..827,852..854)
Misc Feature(11)order(837..839,876..878)
Misc Feature(12)843..845
Misc Feature(13)order(900..902,924..926)
Misc Feature(14)order(912..914,948..950)
Misc Feature(15)942..944
Misc Feature(16)948..1079
Misc Feature(17)order(951..953,975..977)
Misc Feature(18)order(963..965,999..1001)
Misc Feature(19)order(1008..1010,1035..1037)
Misc Feature(20)order(1047..1049,1128..1130)
Misc Feature(21)order(1140..1142,1176..1178)
Misc Feature(22)order(1188..1190,1233..1235)
Misc Feature(23)order(1242..1244,1254..1256)
Misc Feature(24)order(1266..1268,1341..1343)
Misc Feature(25)1311..1313
Misc Feature(26)1338..1700
Misc Feature(27)1338..1340
Misc Feature(28)1425..2057
Misc Feature(29)1494..1496
Misc Feature(30)1587..1589
Misc Feature(31)order(1665..1667,1752..1754)
Misc Feature(32)1770..2168
Misc Feature(33)1773..1934
Misc Feature(34)order(1773..1775,1800..1802)
Misc Feature(35)order(1785..1787,1824..1826)
Misc Feature(36)order(1833..1835,1860..1862)
Misc Feature(37)1839..1841
Misc Feature(38)order(1872..1874,1920..1922)
Misc Feature(39)1911..2051
Misc Feature(40)order(1929..1931,1968..1970)
Misc Feature(41)order(1941..1943,1992..1994)
Misc Feature(42)1959..1961
Misc Feature(43)order(2001..2003,2028..2030)
Misc Feature(44)order(2040..2042,2106..2108)
Misc Feature(45)2064..2066
Misc Feature(46)order(2115..2117,2139..2141)
Misc Feature(47)2124..2126
Misc Feature(48)order(2127..2129,2163..2165)
Misc Feature(49)2157..2288
Misc Feature(50)order(2187..2195,2199..2216,2223..2228)
Misc Feature(51)2193..2261
Misc Feature(52)2289..2291
Misc Feature(53)2289..2291
Misc Feature(54)2319..2369
Misc Feature(55)2334..2336
Misc Feature(56)2334..2336
Misc Feature(57)2340..2342
Misc Feature(58)2367..3305
Misc Feature(59)2394..3161
Misc Feature(60)order(2400..2408,2439..2447,2637..2642,2646..2648,2667..2672,3282..3287)
Misc Feature(61)order(2409..2414,2421..2426,2490..2492,2628..2630,2634..2636,2646..2648,2778..2783,2820..2822,2883..2897,2910..2912,2922..2924)
Misc Feature(62)order(2409..2414,2433..2435,2484..2486,2490..2492,2553..2555,2625..2630,2634..2636,2778..2783,2787..2789,2820..2822)
Misc Feature(63)2436..2438
Misc Feature(64)2559..2561
Misc Feature(65)2817..2894
Misc Feature(66)2862..2864
Misc Feature(67)order(2883..2897,2910..2912,2922..2924)
Misc Feature(68)2928..2930
Misc Feature(69)3000..3002
Misc Feature(70)3087..3089
Misc Feature(71)3189..3191
Misc Feature(72)3228..3230
Misc Feature(73)3228..3230
Misc Feature(74)3240..3242
Misc Feature(75)3240..3242
Misc Feature(76)3249..3251
Misc Feature(77)3249..3251
Misc Feature(78)3303..3305
Misc Feature(79)3303..3305
Misc Feature(80)3303..3305
Misc Feature(81)3303..3305
Misc Feature(82)3303..3305
Misc Feature(83)3333..3335
Misc Feature(84)3333..3335
Misc Feature(85)3363..3365
Misc Feature(86)3366..3368
Misc Feature(87)3372..3374
Misc Feature(88)3372..3374
Misc Feature(89)3378..3380
Misc Feature(90)3378..3380
Misc Feature(91)3381..3383
Misc Feature(92)3381..3383
Misc Feature(93)3390..3392
Misc Feature(94)3447..3449
Misc Feature(95)3447..3449
Misc Feature(96)3462..3464
Misc Feature(97)3462..3464
Misc Feature(98)3462..3464
Misc Feature(99)3462..3464
Misc Feature(100)3462..3464
Misc Feature(101)3465..3467
Misc Feature(102)3465..3467
Misc Feature(103)3465..3467
Misc Feature(104)3468..3470
Misc Feature(105)3468..3470
Misc Feature(106)3468..3470
Misc Feature(107)3498..3500
Misc Feature(108)3498..3500
Misc Feature(109)3531..3533
Misc Feature(110)3531..3533
Misc Feature(111)3531..3533
Misc Feature(112)3585..3587
Misc Feature(113)3585..3587
Misc Feature(114)3615..3617
Misc Feature(115)3630..3632
Misc Feature(116)3669..3671
Misc Feature(117)3753..3755
Misc Feature(118)3753..3755
Misc Feature(119)3771..3773
Misc Feature(120)3771..3773
Misc Feature(121)3846..3848
Misc Feature(122)3846..3848
Misc Feature(123)3846..3848
Misc Feature(124)3852..3854
Exon (1)1..345
Exon (2)346..497
Exon (3)498..681
Exon (4)682..816
Exon (5)817..885
Exon (6)886..1004
Exon (7)1005..1146
Exon (8)1147..1263
Exon (9)1264..1390
Exon (10)1391..1464
Exon (11)1465..1555
Exon (12)1556..1755
Exon (13)1756..1888
Exon (14)1889..1979
Exon (15)1980..2137
Exon (16)2138..2176
Exon (17)2177..2318
Exon (18)2319..2441
Exon (19)2442..2540
Exon (20)2541..2726
Exon (21)2727..2882
Exon (22)2883..2958
Exon (23)2959..3105
Exon (24)3106..3203
Exon (25)3204..3371
Exon (26)3372..3419
Exon (27)3420..3528
Exon (28)3529..6353
Translation

Target ORF information:

RefSeq Version NM_005228.4
Organism Homo sapiens(Human)
Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005228.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
CATCCAAACT GCACCTACGG ATGCACTGGG CCAGGTCTTG AAGGCTGTCC AACGAATGGG
CCTAAGATCC CGTCCATCGC CACTGGGATG GTGGGGGCCC TCCTCTTGCT GCTGGTGGTG
GCCCTGGGGA TCGGCCTCTT CATGCGAAGG CGCCACATCG TTCGGAAGCG CACGCTGCGG
AGGCTGCTGC AGGAGAGGGA GCTTGTGGAG CCTCTTACAC CCAGTGGAGA AGCTCCCAAC
CAAGCTCTCT TGAGGATCTT GAAGGAAACT GAATTCAAAA AGATCAAAGT GCTGGGCTCC
GGTGCGTTCG GCACGGTGTA TAAGGGACTC TGGATCCCAG AAGGTGAGAA AGTTAAAATT
CCCGTCGCTA TCAAGGAATT AAGAGAAGCA ACATCTCCGA AAGCCAACAA GGAAATCCTC
GATGAAGCCT ACGTGATGGC CAGCGTGGAC AACCCCCACG TGTGCCGCCT GCTGGGCATC
TGCCTCACCT CCACCGTGCA GCTCATCACG CAGCTCATGC CCTTCGGCTG CCTCCTGGAC
TATGTCCGGG AACACAAAGA CAATATTGGC TCCCAGTACC TGCTCAACTG GTGTGTGCAG
ATCGCAAAGG GCATGAACTA CTTGGAGGAC CGTCGCTTGG TGCACCGCGA CCTGGCAGCC
AGGAACGTAC TGGTGAAAAC ACCGCAGCAT GTCAAGATCA CAGATTTTGG GCTGGCCAAA
CTGCTGGGTG CGGAAGAGAA AGAATACCAT GCAGAAGGAG GCAAAGTGCC TATCAAGTGG
ATGGCATTGG AATCAATTTT ACACAGAATC TATACCCACC AGAGTGATGT CTGGAGCTAC
GGGGTGACTG TTTGGGAGTT GATGACCTTT GGATCCAAGC CATATGACGG AATCCCTGCC
AGCGAGATCT CCTCCATCCT GGAGAAAGGA GAACGCCTCC CTCAGCCACC CATATGTACC
ATCGATGTCT ACATGATCAT GGTCAAGTGC TGGATGATAG ACGCAGATAG TCGCCCAAAG
TTCCGTGAGT TGATCATCGA ATTCTCCAAA ATGGCCCGAG ACCCCCAGCG CTACCTTGTC
ATTCAGGGGG ATGAAAGAAT GCATTTGCCA AGTCCTACAG ACTCCAACTT CTACCGTGCC
CTGATGGATG AAGAAGACAT GGACGACGTG GTGGATGCCG ACGAGTACCT CATCCCACAG
CAGGGCTTCT TCAGCAGCCC CTCCACGTCA CGGACTCCCC TCCTGAGCTC TCTGAGTGCA
ACCAGCAACA ATTCCACCGT GGCTTGCATT GATAGAAATG GGCTGCAAAG CTGTCCCATC
AAGGAAGACA GCTTCTTGCA GCGATACAGC TCAGACCCCA CAGGCGCCTT GACTGAGGAC
AGCATAGACG ACACCTTCCT CCCAGTGCCT GAATACATAA ACCAGTCCGT TCCCAAAAGG
CCCGCTGGCT CTGTGCAGAA TCCTGTCTAT CACAATCAGC CTCTGAACCC CGCGCCCAGC
AGAGACCCAC ACTACCAGGA CCCCCACAGC ACTGCAGTGG GCAACCCCGA GTATCTCAAC
ACTGTCCAGC CCACCTGTGT CAACAGCACA TTCGACAGCC CTGCCCACTG GGCCCAGAAA
GGCAGCCACC AAATTAGCCT GGACAACCCT GACTACCAGC AGGACTTCTT TCCCAAGGAA
GCCAAGCCAA ATGGCATCTT TAAGGGCTCC ACAGCTGAAA ATGCAGAATA CCTAAGGGTC
GCGCCACAAA GCAGTGAATT TATTGGAGCA TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu102730
Clone ID Related Accession (Same CDS sequence) NM_001346899.1
Accession Version NM_001346899.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3498bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product epidermal growth factor receptor isoform g precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC094761.1, HQ912715.1, AC073324.6, DA371223.1 and AW450657.1. Transcript Variant: This variant (7) lacks an in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (g) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HQ912715.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153307, SAMN03267770 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
CAGGGCCAAA AGTGTGATCC AAGCTGTCCC AATGGGAGCT GCTGGGGTGC AGGAGAGGAG
AACTGCCAGA AACTGACCAA AATCATCTGT GCCCAGCAGT GCTCCGGGCG CTGCCGTGGC
AAGTCCCCCA GTGACTGCTG CCACAACCAG TGTGCTGCAG GCTGCACAGG CCCCCGGGAG
AGCGACTGCC TGGTCTGCCG CAAATTCCGA GACGAAGCCA CGTGCAAGGA CACCTGCCCC
CCACTCATGC TCTACAACCC CACCACGTAC CAGATGGATG TGAACCCCGA GGGCAAATAC
AGCTTTGGTG CCACCTGCGT GAAGAAGTGT CCCCGTAATT ATGTGGTGAC AGATCACGGC
TCGTGCGTCC GAGCCTGTGG GGCCGACAGC TATGAGATGG AGGAAGACGG CGTCCGCAAG
TGTAAGAAGT GCGAAGGGCC TTGCCGCAAA GTGTGTAACG GAATAGGTAT TGGTGAATTT
AAAGACTCAC TCTCCATAAA TGCTACGAAT ATTAAACACT TCAAAAACTG CACCTCCATC
AGTGGCGATC TCCACATCCT GCCGGTGGCA TTTAGGGGTG ACTCCTTCAC ACATACTCCT
CCTCTGGATC CACAGGAACT GGATATTCTG AAAACCGTAA AGGAAATCAC AGGGTTTTTG
CTGATTCAGG CTTGGCCTGA AAACAGGACG GACCTCCATG CCTTTGAGAA CCTAGAAATC
ATACGCGGCA GGACCAAGCA ACATGGTCAG TTTTCTCTTG CAGTCGTCAG CCTGAACATA
ACATCCTTGG GATTACGCTC CCTCAAGGAG ATAAGTGATG GAGATGTGAT AATTTCAGGA
AACAAAAATT TGTGCTATGC AAATACAATA AACTGGAAAA AACTGTTTGG GACCTCCGGT
CAGAAAACCA AAATTATAAG CAACAGAGGT GAAAACAGCT GCAAGGCCAC AGGCCAGGTC
TGCCATGCCT TGTGCTCCCC CGAGGGCTGC TGGGGCCCGG AGCCCAGGGA CTGCGTCTCT
TGCCGGAATG TCAGCCGAGG CAGGGAATGC GTGGACAAGT GCAACCTTCT GGAGGGTGAG
CCAAGGGAGT TTGTGGAGAA CTCTGAGTGC ATACAGTGCC ACCCAGAGTG CCTGCCTCAG
GCCATGAACA TCACCTGCAC AGGACGGGGA CCAGACAACT GTATCCAGTG TGCCCACTAC
ATTGACGGCC CCCACTGCGT CAAGACCTGC CCGGCAGGAG TCATGGGAGA AAACAACACC
CTGGTCTGGA AGTACGCAGA CGCCGGCCAT GTGTGCCACC TGTGCCATCC AAACTGCACC
TACGGATGCA CTGGGCCAGG TCTTGAAGGC TGTCCAACGA ATGGGCCTAA GATCCCGTCC
ATCGCCACTG GGATGGTGGG GGCCCTCCTC TTGCTGCTGG TGGTGGCCCT GGGGATCGGC
CTCTTCATGC GAAGGCGCCA CATCGTTCGG AAGCGCACGC TGCGGAGGCT GCTGCAGGAG
AGGGAGCTTG TGGAGCCTCT TACACCCAGT GGAGAAGCTC CCAACCAAGC TCTCTTGAGG
ATCTTGAAGG AAACTGAATT CAAAAAGATC AAAGTGCTGG GCTCCGGTGC GTTCGGCACG
GTGTATAAGG GACTCTGGAT CCCAGAAGGT GAGAAAGTTA AAATTCCCGT CGCTATCAAG
GAATTAAGAG AAGCAACATC TCCGAAAGCC AACAAGGAAA TCCTCGATGA AGCCTACGTG
ATGGCCAGCG TGGACAACCC CCACGTGTGC CGCCTGCTGG GCATCTGCCT CACCTCCACC
GTGCAGCTCA TCACGCAGCT CATGCCCTTC GGCTGCCTCC TGGACTATGT CCGGGAACAC
AAAGACAATA TTGGCTCCCA GTACCTGCTC AACTGGTGTG TGCAGATCGC AAAGGGCATG
AACTACTTGG AGGACCGTCG CTTGGTGCAC CGCGACCTGG CAGCCAGGAA CGTACTGGTG
AAAACACCGC AGCATGTCAA GATCACAGAT TTTGGGCTGG CCAAACTGCT GGGTGCGGAA
GAGAAAGAAT ACCATGCAGA AGGAGGCAAA GTGCCTATCA AGTGGATGGC ATTGGAATCA
ATTTTACACA GAATCTATAC CCACCAGAGT GATGTCTGGA GCTACGGGGT GACTGTTTGG
GAGTTGATGA CCTTTGGATC CAAGCCATAT GACGGAATCC CTGCCAGCGA GATCTCCTCC
ATCCTGGAGA AAGGAGAACG CCTCCCTCAG CCACCCATAT GTACCATCGA TGTCTACATG
ATCATGGTCA AGTGCTGGAT GATAGACGCA GATAGTCGCC CAAAGTTCCG TGAGTTGATC
ATCGAATTCT CCAAAATGGC CCGAGACCCC CAGCGCTACC TTGTCATTCA GGGGGATGAA
AGAATGCATT TGCCAAGTCC TACAGACTCC AACTTCTACC GTGCCCTGAT GGATGAAGAA
GACATGGACG ACGTGGTGGA TGCCGACGAG TACCTCATCC CACAGCAGGG CTTCTTCAGC
AGCCCCTCCA CGTCACGGAC TCCCCTCCTG AGCTCTCTGA GTGCAACCAG CAACAATTCC
ACCGTGGCTT GCATTGATAG AAATGGGCTG CAAAGCTGTC CCATCAAGGA AGACAGCTTC
TTGCAGCGAT ACAGCTCAGA CCCCACAGGC GCCTTGACTG AGGACAGCAT AGACGACACC
TTCCTCCCAG TGCCTGAATA CATAAACCAG TCCGTTCCCA AAAGGCCCGC TGGCTCTGTG
CAGAATCCTG TCTATCACAA TCAGCCTCTG AACCCCGCGC CCAGCAGAGA CCCACACTAC
CAGGACCCCC ACAGCACTGC AGTGGGCAAC CCCGAGTATC TCAACACTGT CCAGCCCACC
TGTGTCAACA GCACATTCGA CAGCCCTGCC CACTGGGCCC AGAAAGGCAG CCACCAAATT
AGCCTGGACA ACCCTGACTA CCAGCAGGAC TTCTTTCCCA AGGAAGCCAA GCCAAATGGC
ATCTTTAAGG GCTCCACAGC TGAAAATGCA GAATACCTAA GGGTCGCGCC ACAAAGCAGT
GAATTTATTG GAGCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001333828.1
CDS258..3755
Misc Feature(1)219..221
Exon (1)1..345
Exon (2)346..497
Exon (3)498..681
Exon (4)682..750
Exon (5)751..869
Exon (6)870..1011
Exon (7)1012..1128
Exon (8)1129..1255
Exon (9)1256..1329
Exon (10)1330..1420
Exon (11)1421..1620
Exon (12)1621..1753
Exon (13)1754..1844
Exon (14)1845..2002
Exon (15)2003..2041
Exon (16)2042..2183
Exon (17)2184..2306
Exon (18)2307..2405
Exon (19)2406..2591
Exon (20)2592..2747
Exon (21)2748..2823
Exon (22)2824..2970
Exon (23)2971..3068
Exon (24)3069..3236
Exon (25)3237..3284
Exon (26)3285..3393
Exon (27)3394..6218
Translation

Target ORF information:

RefSeq Version NM_001346899.1
Organism Homo sapiens(Human)
Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001346899.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
CAGGGCCAAA AGTGTGATCC AAGCTGTCCC AATGGGAGCT GCTGGGGTGC AGGAGAGGAG
AACTGCCAGA AACTGACCAA AATCATCTGT GCCCAGCAGT GCTCCGGGCG CTGCCGTGGC
AAGTCCCCCA GTGACTGCTG CCACAACCAG TGTGCTGCAG GCTGCACAGG CCCCCGGGAG
AGCGACTGCC TGGTCTGCCG CAAATTCCGA GACGAAGCCA CGTGCAAGGA CACCTGCCCC
CCACTCATGC TCTACAACCC CACCACGTAC CAGATGGATG TGAACCCCGA GGGCAAATAC
AGCTTTGGTG CCACCTGCGT GAAGAAGTGT CCCCGTAATT ATGTGGTGAC AGATCACGGC
TCGTGCGTCC GAGCCTGTGG GGCCGACAGC TATGAGATGG AGGAAGACGG CGTCCGCAAG
TGTAAGAAGT GCGAAGGGCC TTGCCGCAAA GTGTGTAACG GAATAGGTAT TGGTGAATTT
AAAGACTCAC TCTCCATAAA TGCTACGAAT ATTAAACACT TCAAAAACTG CACCTCCATC
AGTGGCGATC TCCACATCCT GCCGGTGGCA TTTAGGGGTG ACTCCTTCAC ACATACTCCT
CCTCTGGATC CACAGGAACT GGATATTCTG AAAACCGTAA AGGAAATCAC AGGGTTTTTG
CTGATTCAGG CTTGGCCTGA AAACAGGACG GACCTCCATG CCTTTGAGAA CCTAGAAATC
ATACGCGGCA GGACCAAGCA ACATGGTCAG TTTTCTCTTG CAGTCGTCAG CCTGAACATA
ACATCCTTGG GATTACGCTC CCTCAAGGAG ATAAGTGATG GAGATGTGAT AATTTCAGGA
AACAAAAATT TGTGCTATGC AAATACAATA AACTGGAAAA AACTGTTTGG GACCTCCGGT
CAGAAAACCA AAATTATAAG CAACAGAGGT GAAAACAGCT GCAAGGCCAC AGGCCAGGTC
TGCCATGCCT TGTGCTCCCC CGAGGGCTGC TGGGGCCCGG AGCCCAGGGA CTGCGTCTCT
TGCCGGAATG TCAGCCGAGG CAGGGAATGC GTGGACAAGT GCAACCTTCT GGAGGGTGAG
CCAAGGGAGT TTGTGGAGAA CTCTGAGTGC ATACAGTGCC ACCCAGAGTG CCTGCCTCAG
GCCATGAACA TCACCTGCAC AGGACGGGGA CCAGACAACT GTATCCAGTG TGCCCACTAC
ATTGACGGCC CCCACTGCGT CAAGACCTGC CCGGCAGGAG TCATGGGAGA AAACAACACC
CTGGTCTGGA AGTACGCAGA CGCCGGCCAT GTGTGCCACC TGTGCCATCC AAACTGCACC
TACGGATGCA CTGGGCCAGG TCTTGAAGGC TGTCCAACGA ATGGGCCTAA GATCCCGTCC
ATCGCCACTG GGATGGTGGG GGCCCTCCTC TTGCTGCTGG TGGTGGCCCT GGGGATCGGC
CTCTTCATGC GAAGGCGCCA CATCGTTCGG AAGCGCACGC TGCGGAGGCT GCTGCAGGAG
AGGGAGCTTG TGGAGCCTCT TACACCCAGT GGAGAAGCTC CCAACCAAGC TCTCTTGAGG
ATCTTGAAGG AAACTGAATT CAAAAAGATC AAAGTGCTGG GCTCCGGTGC GTTCGGCACG
GTGTATAAGG GACTCTGGAT CCCAGAAGGT GAGAAAGTTA AAATTCCCGT CGCTATCAAG
GAATTAAGAG AAGCAACATC TCCGAAAGCC AACAAGGAAA TCCTCGATGA AGCCTACGTG
ATGGCCAGCG TGGACAACCC CCACGTGTGC CGCCTGCTGG GCATCTGCCT CACCTCCACC
GTGCAGCTCA TCACGCAGCT CATGCCCTTC GGCTGCCTCC TGGACTATGT CCGGGAACAC
AAAGACAATA TTGGCTCCCA GTACCTGCTC AACTGGTGTG TGCAGATCGC AAAGGGCATG
AACTACTTGG AGGACCGTCG CTTGGTGCAC CGCGACCTGG CAGCCAGGAA CGTACTGGTG
AAAACACCGC AGCATGTCAA GATCACAGAT TTTGGGCTGG CCAAACTGCT GGGTGCGGAA
GAGAAAGAAT ACCATGCAGA AGGAGGCAAA GTGCCTATCA AGTGGATGGC ATTGGAATCA
ATTTTACACA GAATCTATAC CCACCAGAGT GATGTCTGGA GCTACGGGGT GACTGTTTGG
GAGTTGATGA CCTTTGGATC CAAGCCATAT GACGGAATCC CTGCCAGCGA GATCTCCTCC
ATCCTGGAGA AAGGAGAACG CCTCCCTCAG CCACCCATAT GTACCATCGA TGTCTACATG
ATCATGGTCA AGTGCTGGAT GATAGACGCA GATAGTCGCC CAAAGTTCCG TGAGTTGATC
ATCGAATTCT CCAAAATGGC CCGAGACCCC CAGCGCTACC TTGTCATTCA GGGGGATGAA
AGAATGCATT TGCCAAGTCC TACAGACTCC AACTTCTACC GTGCCCTGAT GGATGAAGAA
GACATGGACG ACGTGGTGGA TGCCGACGAG TACCTCATCC CACAGCAGGG CTTCTTCAGC
AGCCCCTCCA CGTCACGGAC TCCCCTCCTG AGCTCTCTGA GTGCAACCAG CAACAATTCC
ACCGTGGCTT GCATTGATAG AAATGGGCTG CAAAGCTGTC CCATCAAGGA AGACAGCTTC
TTGCAGCGAT ACAGCTCAGA CCCCACAGGC GCCTTGACTG AGGACAGCAT AGACGACACC
TTCCTCCCAG TGCCTGAATA CATAAACCAG TCCGTTCCCA AAAGGCCCGC TGGCTCTGTG
CAGAATCCTG TCTATCACAA TCAGCCTCTG AACCCCGCGC CCAGCAGAGA CCCACACTAC
CAGGACCCCC ACAGCACTGC AGTGGGCAAC CCCGAGTATC TCAACACTGT CCAGCCCACC
TGTGTCAACA GCACATTCGA CAGCCCTGCC CACTGGGCCC AGAAAGGCAG CCACCAAATT
AGCCTGGACA ACCCTGACTA CCAGCAGGAC TTCTTTCCCA AGGAAGCCAA GCCAAATGGC
ATCTTTAAGG GCTCCACAGC TGAAAATGCA GAATACCTAA GGGTCGCGCC ACAAAGCAGT
GAATTTATTG GAGCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu102762
Clone ID Related Accession (Same CDS sequence) NM_001346898.1
Accession Version NM_001346898.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3411bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product epidermal growth factor receptor isoform f precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC094761.1, HQ912715.1, GU255993.1 and AC073324.6. Transcript Variant: This variant (6) has a 3' terminal exon that extends past a splice site that is used in variant 1. The encoded isoform (f) has a shorter and distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GU255993.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
CATCCAAACT GCACCTACGG ATGCACTGGG CCAGGTCTTG AAGGCTGTCC AACGAATGGG
CCTAAGATCC CGTCCATCGC CACTGGGATG GTGGGGGCCC TCCTCTTGCT GCTGGTGGTG
GCCCTGGGGA TCGGCCTCTT CATGCGAAGG CGCCACATCG TTCGGAAGCG CACGCTGCGG
AGGCTGCTGC AGGAGAGGGA GCTTGTGGAG CCTCTTACAC CCAGTGGAGA AGCTCCCAAC
CAAGCTCTCT TGAGGATCTT GAAGGAAACT GAATTCAAAA AGATCAAAGT GCTGGGCTCC
GGTGCGTTCG GCACGGTGTA TAAGGGACTC TGGATCCCAG AAGGTGAGAA AGTTAAAATT
CCCGTCGCTA TCAAGGAATT AAGAGAAGCA ACATCTCCGA AAGCCAACAA GGAAATCCTC
GATGAAGCCT ACGTGATGGC CAGCGTGGAC AACCCCCACG TGTGCCGCCT GCTGGGCATC
TGCCTCACCT CCACCGTGCA GCTCATCACG CAGCTCATGC CCTTCGGCTG CCTCCTGGAC
TATGTCCGGG AACACAAAGA CAATATTGGC TCCCAGTACC TGCTCAACTG GTGTGTGCAG
ATCGCAAAGG GCATGAACTA CTTGGAGGAC CGTCGCTTGG TGCACCGCGA CCTGGCAGCC
AGGAACGTAC TGGTGAAAAC ACCGCAGCAT GTCAAGATCA CAGATTTTGG GCTGGCCAAA
CTGCTGGGTG CGGAAGAGAA AGAATACCAT GCAGAAGGAG GCAAAGTGCC TATCAAGTGG
ATGGCATTGG AATCAATTTT ACACAGAATC TATACCCACC AGAGTGATGT CTGGAGCTAC
GGGGTGACTG TTTGGGAGTT GATGACCTTT GGATCCAAGC CATATGACGG AATCCCTGCC
AGCGAGATCT CCTCCATCCT GGAGAAAGGA GAACGCCTCC CTCAGCCACC CATATGTACC
ATCGATGTCT ACATGATCAT GGTCAAGTGC TGGATGATAG ACGCAGATAG TCGCCCAAAG
TTCCGTGAGT TGATCATCGA ATTCTCCAAA ATGGCCCGAG ACCCCCAGCG CTACCTTGTC
ATTCAGGGGG ATGAAAGAAT GCATTTGCCA AGTCCTACAG ACTCCAACTT CTACCGTGCC
CTGATGGATG AAGAAGACAT GGACGACGTG GTGGATGCCG ACGAGTACCT CATCCCACAG
CAGGGCTTCT TCAGCAGCCC CTCCACGTCA CGGACTCCCC TCCTGAGCTC TCTGAGTGCA
ACCAGCAACA ATTCCACCGT GGCTTGCATT GATAGAAATG GGCTGCAAAG CTGTCCCATC
AAGGAAGACA GCTTCTTGCA GCGATACAGC TCAGACCCCA CAGGCGCCTT GACTGAGGAC
AGCATAGACG ACACCTTCCT CCCAGTGCCT GGTGAGTGGC TTGTCTGGAA ACAGTCCTGC
TCCTCAACCT CCTCGACCCA CTCAGCAGCA GCCAGTCTCC AGTGTCCAAG CCAGGTGCTC
CCTCCAGCAT CTCCAGAGGG GGAAACAGTG GCAGATTTGC AGACACAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001333827.1
CDS258..3668
Misc Feature(1)219..221
Misc Feature(2)480..1157
Misc Feature(3)942..944
Misc Feature(4)1425..2057
Misc Feature(5)2193..2261
Misc Feature(6)2289..2291
Misc Feature(7)2319..2369
Misc Feature(8)2334..2336
Misc Feature(9)2340..2342
Misc Feature(10)3228..3230
Misc Feature(11)3240..3242
Misc Feature(12)3249..3251
Misc Feature(13)3303..3305
Misc Feature(14)3303..3305
Misc Feature(15)3333..3335
Misc Feature(16)3372..3374
Misc Feature(17)3378..3380
Misc Feature(18)3381..3383
Misc Feature(19)3447..3449
Misc Feature(20)3462..3464
Misc Feature(21)3465..3467
Misc Feature(22)3468..3470
Misc Feature(23)3498..3500
Exon (1)1..345
Exon (2)346..497
Exon (3)498..681
Exon (4)682..816
Exon (5)817..885
Exon (6)886..1004
Exon (7)1005..1146
Exon (8)1147..1263
Exon (9)1264..1390
Exon (10)1391..1464
Exon (11)1465..1555
Exon (12)1556..1755
Exon (13)1756..1888
Exon (14)1889..1979
Exon (15)1980..2137
Exon (16)2138..2176
Exon (17)2177..2318
Exon (18)2319..2441
Exon (19)2442..2540
Exon (20)2541..2726
Exon (21)2727..2882
Exon (22)2883..2958
Exon (23)2959..3105
Exon (24)3106..3203
Exon (25)3204..3371
Exon (26)3372..3419
Exon (27)3420..3979
Translation

Target ORF information:

RefSeq Version NM_001346898.1
Organism Homo sapiens(Human)
Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001346898.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
CATCCAAACT GCACCTACGG ATGCACTGGG CCAGGTCTTG AAGGCTGTCC AACGAATGGG
CCTAAGATCC CGTCCATCGC CACTGGGATG GTGGGGGCCC TCCTCTTGCT GCTGGTGGTG
GCCCTGGGGA TCGGCCTCTT CATGCGAAGG CGCCACATCG TTCGGAAGCG CACGCTGCGG
AGGCTGCTGC AGGAGAGGGA GCTTGTGGAG CCTCTTACAC CCAGTGGAGA AGCTCCCAAC
CAAGCTCTCT TGAGGATCTT GAAGGAAACT GAATTCAAAA AGATCAAAGT GCTGGGCTCC
GGTGCGTTCG GCACGGTGTA TAAGGGACTC TGGATCCCAG AAGGTGAGAA AGTTAAAATT
CCCGTCGCTA TCAAGGAATT AAGAGAAGCA ACATCTCCGA AAGCCAACAA GGAAATCCTC
GATGAAGCCT ACGTGATGGC CAGCGTGGAC AACCCCCACG TGTGCCGCCT GCTGGGCATC
TGCCTCACCT CCACCGTGCA GCTCATCACG CAGCTCATGC CCTTCGGCTG CCTCCTGGAC
TATGTCCGGG AACACAAAGA CAATATTGGC TCCCAGTACC TGCTCAACTG GTGTGTGCAG
ATCGCAAAGG GCATGAACTA CTTGGAGGAC CGTCGCTTGG TGCACCGCGA CCTGGCAGCC
AGGAACGTAC TGGTGAAAAC ACCGCAGCAT GTCAAGATCA CAGATTTTGG GCTGGCCAAA
CTGCTGGGTG CGGAAGAGAA AGAATACCAT GCAGAAGGAG GCAAAGTGCC TATCAAGTGG
ATGGCATTGG AATCAATTTT ACACAGAATC TATACCCACC AGAGTGATGT CTGGAGCTAC
GGGGTGACTG TTTGGGAGTT GATGACCTTT GGATCCAAGC CATATGACGG AATCCCTGCC
AGCGAGATCT CCTCCATCCT GGAGAAAGGA GAACGCCTCC CTCAGCCACC CATATGTACC
ATCGATGTCT ACATGATCAT GGTCAAGTGC TGGATGATAG ACGCAGATAG TCGCCCAAAG
TTCCGTGAGT TGATCATCGA ATTCTCCAAA ATGGCCCGAG ACCCCCAGCG CTACCTTGTC
ATTCAGGGGG ATGAAAGAAT GCATTTGCCA AGTCCTACAG ACTCCAACTT CTACCGTGCC
CTGATGGATG AAGAAGACAT GGACGACGTG GTGGATGCCG ACGAGTACCT CATCCCACAG
CAGGGCTTCT TCAGCAGCCC CTCCACGTCA CGGACTCCCC TCCTGAGCTC TCTGAGTGCA
ACCAGCAACA ATTCCACCGT GGCTTGCATT GATAGAAATG GGCTGCAAAG CTGTCCCATC
AAGGAAGACA GCTTCTTGCA GCGATACAGC TCAGACCCCA CAGGCGCCTT GACTGAGGAC
AGCATAGACG ACACCTTCCT CCCAGTGCCT GGTGAGTGGC TTGTCTGGAA ACAGTCCTGC
TCCTCAACCT CCTCGACCCA CTCAGCAGCA GCCAGTCTCC AGTGTCCAAG CCAGGTGCTC
CCTCCAGCAT CTCCAGAGGG GGAAACAGTG GCAGATTTGC AGACACAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu102780
Clone ID Related Accession (Same CDS sequence) NM_001346897.1
Accession Version NM_001346897.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product epidermal growth factor receptor isoform e precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC094761.1, AC006977.3 and AC073324.6. Transcript Variant: This variant (5) lacks an in-frame exon in the 5' coding region and its 3' terminal exon extends past a splice site that is used in variant 1. The encoded isoform (e) is shorter and has a distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC094761.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153307, SAMN03267770 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
CAGGGCCAAA AGTGTGATCC AAGCTGTCCC AATGGGAGCT GCTGGGGTGC AGGAGAGGAG
AACTGCCAGA AACTGACCAA AATCATCTGT GCCCAGCAGT GCTCCGGGCG CTGCCGTGGC
AAGTCCCCCA GTGACTGCTG CCACAACCAG TGTGCTGCAG GCTGCACAGG CCCCCGGGAG
AGCGACTGCC TGGTCTGCCG CAAATTCCGA GACGAAGCCA CGTGCAAGGA CACCTGCCCC
CCACTCATGC TCTACAACCC CACCACGTAC CAGATGGATG TGAACCCCGA GGGCAAATAC
AGCTTTGGTG CCACCTGCGT GAAGAAGTGT CCCCGTAATT ATGTGGTGAC AGATCACGGC
TCGTGCGTCC GAGCCTGTGG GGCCGACAGC TATGAGATGG AGGAAGACGG CGTCCGCAAG
TGTAAGAAGT GCGAAGGGCC TTGCCGCAAA GTGTGTAACG GAATAGGTAT TGGTGAATTT
AAAGACTCAC TCTCCATAAA TGCTACGAAT ATTAAACACT TCAAAAACTG CACCTCCATC
AGTGGCGATC TCCACATCCT GCCGGTGGCA TTTAGGGGTG ACTCCTTCAC ACATACTCCT
CCTCTGGATC CACAGGAACT GGATATTCTG AAAACCGTAA AGGAAATCAC AGGGTTTTTG
CTGATTCAGG CTTGGCCTGA AAACAGGACG GACCTCCATG CCTTTGAGAA CCTAGAAATC
ATACGCGGCA GGACCAAGCA ACATGGTCAG TTTTCTCTTG CAGTCGTCAG CCTGAACATA
ACATCCTTGG GATTACGCTC CCTCAAGGAG ATAAGTGATG GAGATGTGAT AATTTCAGGA
AACAAAAATT TGTGCTATGC AAATACAATA AACTGGAAAA AACTGTTTGG GACCTCCGGT
CAGAAAACCA AAATTATAAG CAACAGAGGT GAAAACAGCT GCAAGGCCAC AGGCCAGGTC
TGCCATGCCT TGTGCTCCCC CGAGGGCTGC TGGGGCCCGG AGCCCAGGGA CTGCGTCTCT
TGCCGGAATG TCAGCCGAGG CAGGGAATGC GTGGACAAGT GCAACCTTCT GGAGGGTGAG
CCAAGGGAGT TTGTGGAGAA CTCTGAGTGC ATACAGTGCC ACCCAGAGTG CCTGCCTCAG
GCCATGAACA TCACCTGCAC AGGACGGGGA CCAGACAACT GTATCCAGTG TGCCCACTAC
ATTGACGGCC CCCACTGCGT CAAGACCTGC CCGGCAGGAG TCATGGGAGA AAACAACACC
CTGGTCTGGA AGTACGCAGA CGCCGGCCAT GTGTGCCACC TGTGCCATCC AAACTGCACC
TACGGATGCA CTGGGCCAGG TCTTGAAGGC TGTCCAACGA ATGGGCCTAA GATCCCGTCC
ATCGCCACTG GGATGGTGGG GGCCCTCCTC TTGCTGCTGG TGGTGGCCCT GGGGATCGGC
CTCTTCATGC GAAGGCGCCA CATCGTTCGG AAGCGCACGC TGCGGAGGCT GCTGCAGGAG
AGGGAGCTTG TGGAGCCTCT TACACCCAGT GGAGAAGCTC CCAACCAAGC TCTCTTGAGG
ATCTTGAAGG AAACTGAATT CAAAAAGATC AAAGTGCTGG GCTCCGGTGC GTTCGGCACG
GTGTATAAGG GACTCTGGAT CCCAGAAGGT GAGAAAGTTA AAATTCCCGT CGCTATCAAG
GAATTAAGAG AAGCAACATC TCCGAAAGCC AACAAGGAAA TCCTCGATGA AGCCTACGTG
ATGGCCAGCG TGGACAACCC CCACGTGTGC CGCCTGCTGG GCATCTGCCT CACCTCCACC
GTGCAGCTCA TCACGCAGCT CATGCCCTTC GGCTGCCTCC TGGACTATGT CCGGGAACAC
AAAGACAATA TTGGCTCCCA GTACCTGCTC AACTGGTGTG TGCAGATCGC AAAGGGCATG
AACTACTTGG AGGACCGTCG CTTGGTGCAC CGCGACCTGG CAGCCAGGAA CGTACTGGTG
AAAACACCGC AGCATGTCAA GATCACAGAT TTTGGGCTGG CCAAACTGCT GGGTGCGGAA
GAGAAAGAAT ACCATGCAGA AGGAGGCAAA GTGCCTATCA AGTGGATGGC ATTGGAATCA
ATTTTACACA GAATCTATAC CCACCAGAGT GATGTCTGGA GCTACGGGGT GACTGTTTGG
GAGTTGATGA CCTTTGGATC CAAGCCATAT GACGGAATCC CTGCCAGCGA GATCTCCTCC
ATCCTGGAGA AAGGAGAACG CCTCCCTCAG CCACCCATAT GTACCATCGA TGTCTACATG
ATCATGGTCA AGTGCTGGAT GATAGACGCA GATAGTCGCC CAAAGTTCCG TGAGTTGATC
ATCGAATTCT CCAAAATGGC CCGAGACCCC CAGCGCTACC TTGTCATTCA GGGGGATGAA
AGAATGCATT TGCCAAGTCC TACAGACTCC AACTTCTACC GTGCCCTGAT GGATGAAGAA
GACATGGACG ACGTGGTGGA TGCCGACGAG TACCTCATCC CACAGCAGGG CTTCTTCAGC
AGCCCCTCCA CGTCACGGAC TCCCCTCCTG AGCTCTCTGA GTGCAACCAG CAACAATTCC
ACCGTGGCTT GCATTGATAG AAATGGGCTG CAAAGCTGTC CCATCAAGGA AGACAGCTTC
TTGCAGCGAT ACAGCTCAGA CCCCACAGGC GCCTTGACTG AGGACAGCAT AGACGACACC
TTCCTCCCAG TGCCTGGTGA GTGGCTTGTC TGGAAACAGT CCTGCTCCTC AACCTCCTCG
ACCCACTCAG CAGCAGCCAG TCTCCAGTGT CCAAGCCAGG TGCTCCCTCC AGCATCTCCA
GAGGGGGAAA CAGTGGCAGA TTTGCAGACA CAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001333826.1
CDS258..3533
Misc Feature(1)219..221
Exon (1)1..345
Exon (2)346..497
Exon (3)498..681
Exon (4)682..750
Exon (5)751..869
Exon (6)870..1011
Exon (7)1012..1128
Exon (8)1129..1255
Exon (9)1256..1329
Exon (10)1330..1420
Exon (11)1421..1620
Exon (12)1621..1753
Exon (13)1754..1844
Exon (14)1845..2002
Exon (15)2003..2041
Exon (16)2042..2183
Exon (17)2184..2306
Exon (18)2307..2405
Exon (19)2406..2591
Exon (20)2592..2747
Exon (21)2748..2823
Exon (22)2824..2970
Exon (23)2971..3068
Exon (24)3069..3236
Exon (25)3237..3284
Exon (26)3285..3844
Translation

Target ORF information:

RefSeq Version NM_001346897.1
Organism Homo sapiens(Human)
Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001346897.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
CAGGGCCAAA AGTGTGATCC AAGCTGTCCC AATGGGAGCT GCTGGGGTGC AGGAGAGGAG
AACTGCCAGA AACTGACCAA AATCATCTGT GCCCAGCAGT GCTCCGGGCG CTGCCGTGGC
AAGTCCCCCA GTGACTGCTG CCACAACCAG TGTGCTGCAG GCTGCACAGG CCCCCGGGAG
AGCGACTGCC TGGTCTGCCG CAAATTCCGA GACGAAGCCA CGTGCAAGGA CACCTGCCCC
CCACTCATGC TCTACAACCC CACCACGTAC CAGATGGATG TGAACCCCGA GGGCAAATAC
AGCTTTGGTG CCACCTGCGT GAAGAAGTGT CCCCGTAATT ATGTGGTGAC AGATCACGGC
TCGTGCGTCC GAGCCTGTGG GGCCGACAGC TATGAGATGG AGGAAGACGG CGTCCGCAAG
TGTAAGAAGT GCGAAGGGCC TTGCCGCAAA GTGTGTAACG GAATAGGTAT TGGTGAATTT
AAAGACTCAC TCTCCATAAA TGCTACGAAT ATTAAACACT TCAAAAACTG CACCTCCATC
AGTGGCGATC TCCACATCCT GCCGGTGGCA TTTAGGGGTG ACTCCTTCAC ACATACTCCT
CCTCTGGATC CACAGGAACT GGATATTCTG AAAACCGTAA AGGAAATCAC AGGGTTTTTG
CTGATTCAGG CTTGGCCTGA AAACAGGACG GACCTCCATG CCTTTGAGAA CCTAGAAATC
ATACGCGGCA GGACCAAGCA ACATGGTCAG TTTTCTCTTG CAGTCGTCAG CCTGAACATA
ACATCCTTGG GATTACGCTC CCTCAAGGAG ATAAGTGATG GAGATGTGAT AATTTCAGGA
AACAAAAATT TGTGCTATGC AAATACAATA AACTGGAAAA AACTGTTTGG GACCTCCGGT
CAGAAAACCA AAATTATAAG CAACAGAGGT GAAAACAGCT GCAAGGCCAC AGGCCAGGTC
TGCCATGCCT TGTGCTCCCC CGAGGGCTGC TGGGGCCCGG AGCCCAGGGA CTGCGTCTCT
TGCCGGAATG TCAGCCGAGG CAGGGAATGC GTGGACAAGT GCAACCTTCT GGAGGGTGAG
CCAAGGGAGT TTGTGGAGAA CTCTGAGTGC ATACAGTGCC ACCCAGAGTG CCTGCCTCAG
GCCATGAACA TCACCTGCAC AGGACGGGGA CCAGACAACT GTATCCAGTG TGCCCACTAC
ATTGACGGCC CCCACTGCGT CAAGACCTGC CCGGCAGGAG TCATGGGAGA AAACAACACC
CTGGTCTGGA AGTACGCAGA CGCCGGCCAT GTGTGCCACC TGTGCCATCC AAACTGCACC
TACGGATGCA CTGGGCCAGG TCTTGAAGGC TGTCCAACGA ATGGGCCTAA GATCCCGTCC
ATCGCCACTG GGATGGTGGG GGCCCTCCTC TTGCTGCTGG TGGTGGCCCT GGGGATCGGC
CTCTTCATGC GAAGGCGCCA CATCGTTCGG AAGCGCACGC TGCGGAGGCT GCTGCAGGAG
AGGGAGCTTG TGGAGCCTCT TACACCCAGT GGAGAAGCTC CCAACCAAGC TCTCTTGAGG
ATCTTGAAGG AAACTGAATT CAAAAAGATC AAAGTGCTGG GCTCCGGTGC GTTCGGCACG
GTGTATAAGG GACTCTGGAT CCCAGAAGGT GAGAAAGTTA AAATTCCCGT CGCTATCAAG
GAATTAAGAG AAGCAACATC TCCGAAAGCC AACAAGGAAA TCCTCGATGA AGCCTACGTG
ATGGCCAGCG TGGACAACCC CCACGTGTGC CGCCTGCTGG GCATCTGCCT CACCTCCACC
GTGCAGCTCA TCACGCAGCT CATGCCCTTC GGCTGCCTCC TGGACTATGT CCGGGAACAC
AAAGACAATA TTGGCTCCCA GTACCTGCTC AACTGGTGTG TGCAGATCGC AAAGGGCATG
AACTACTTGG AGGACCGTCG CTTGGTGCAC CGCGACCTGG CAGCCAGGAA CGTACTGGTG
AAAACACCGC AGCATGTCAA GATCACAGAT TTTGGGCTGG CCAAACTGCT GGGTGCGGAA
GAGAAAGAAT ACCATGCAGA AGGAGGCAAA GTGCCTATCA AGTGGATGGC ATTGGAATCA
ATTTTACACA GAATCTATAC CCACCAGAGT GATGTCTGGA GCTACGGGGT GACTGTTTGG
GAGTTGATGA CCTTTGGATC CAAGCCATAT GACGGAATCC CTGCCAGCGA GATCTCCTCC
ATCCTGGAGA AAGGAGAACG CCTCCCTCAG CCACCCATAT GTACCATCGA TGTCTACATG
ATCATGGTCA AGTGCTGGAT GATAGACGCA GATAGTCGCC CAAAGTTCCG TGAGTTGATC
ATCGAATTCT CCAAAATGGC CCGAGACCCC CAGCGCTACC TTGTCATTCA GGGGGATGAA
AGAATGCATT TGCCAAGTCC TACAGACTCC AACTTCTACC GTGCCCTGAT GGATGAAGAA
GACATGGACG ACGTGGTGGA TGCCGACGAG TACCTCATCC CACAGCAGGG CTTCTTCAGC
AGCCCCTCCA CGTCACGGAC TCCCCTCCTG AGCTCTCTGA GTGCAACCAG CAACAATTCC
ACCGTGGCTT GCATTGATAG AAATGGGCTG CAAAGCTGTC CCATCAAGGA AGACAGCTTC
TTGCAGCGAT ACAGCTCAGA CCCCACAGGC GCCTTGACTG AGGACAGCAT AGACGACACC
TTCCTCCCAG TGCCTGGTGA GTGGCTTGTC TGGAAACAGT CCTGCTCCTC AACCTCCTCG
ACCCACTCAG CAGCAGCCAG TCTCCAGTGT CCAAGCCAGG TGCTCCCTCC AGCATCTCCA
GAGGGGGAAA CAGTGGCAGA TTTGCAGACA CAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Epidermal growth factor receptor mutations should be considered as a prognostic factor for survival of patients with pathological fractures or painful bone metastases from non-small cell lung cancer.
The bone & joint journal99-B(4)516-521(2017 Apr)
Willeumier JJ,van der Hoeven NM,Bollen L,Willems LN,Fiocco M,van der Linden YM,Dijkstra PD


book

Impact of epidermal growth factor receptor gene expression level on clinical outcomes in epidermal growth factor receptor mutant lung adenocarcinoma patients taking first-line epidermal growth factor receptor-tyrosine kinase inhibitors.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(3)1010428317695939(2017 Mar)
Chang HC,Chen YM,Tseng CC,Huang KT,Wang CC,Chen YC,Lai CH,Fang WF,Kao HC,Lin MC


book

Correlation of EGFR or KRAS mutation status with 18F-FDG uptake on PET-CT scan in lung adenocarcinoma.
PloS one12(4)e0175622(2017)
Takamochi K,Mogushi K,Kawaji H,Imashimizu K,Fukui M,Oh S,Itoh M,Hayashizaki Y,Ko W,Akeboshi M,Suzuki K


book

Expression, Mutation, and Amplification Status of EGFR and Its Correlation with Five miRNAs in Salivary Gland Tumours.
BioMed research international20179150402(2017)
Boštjančič E,Hauptman N,Grošelj A,Glavač D,Volavšek M


book

The epidermal growth factor receptor variant III (EGFRvIII): where wild things are altered.
The FEBS journal280(21)5350-70(2013 Nov)
Gan HK,Cvrljevic AN,Johns TG


 
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