EGFR cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following EGFR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EGFR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu25365 NM_201282.2
Latest version!
Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu25409 NM_201283.1
Latest version!
Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$169.50-$237.30
$339.00
OHu25437 NM_005228.3
Latest version!
Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$609.50-$853.30
$1219.00
OHu25398 NM_201284.2
Latest version!
Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $496.30
$709.00
OHu102763 NM_001346941.2
Latest version!
Homo sapiens epidermal growth factor receptor (EGFR), transcript variant EGFRvIII, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu102753 NM_001346900.2
Latest version!
Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $559.30
$799.00
OHu102757 NM_005228.5
Latest version!
Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $643.30
$919.00
OHu102730 NM_001346899.1
Latest version!
Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu102762 NM_001346898.2
Latest version!
Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu102780 NM_001346897.2
Latest version!
Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
View Old Accession Versions >>
OHu102763 NM_001346941.1 Homo sapiens epidermal growth factor receptor (EGFR), transcript variant EGFRvIII, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu102762 NM_001346898.1 Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu102757 NM_005228.4 Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $643.30
$919.00
OHu102780 NM_001346897.1 Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu102753 NM_001346900.1 Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $559.30
$799.00
OHu25365 NM_201282.1 Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu25398 NM_201284.1 Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $496.30
$709.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu25365
    Clone ID Related Accession (Same CDS sequence) NM_201282.1 , NM_201282.2
    Accession Version NM_201282.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1887bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product epidermal growth factor receptor isoform b precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AW163038.1, X00588.1, AF125253.1 and AF277897.1. Transcript Variant: This variant (2) uses a different 3' terminal exon when compared to variant 1. The resulting isoform (b) has a shorter and distinct C-terminus. Only the extracellular domain is present in isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 11161793 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
    AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
    CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
    GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
    GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
    ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
    AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
    CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
    GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
    GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
    GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
    AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
    TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
    ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
    GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
    GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
    GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
    TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
    GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
    AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
    CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
    GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
    CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
    GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
    CATCCAAACT GCACCTACGG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_958439.1
    CDS247..2133
    Translation

    Target ORF information:

    RefSeq Version NM_201282.1
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_201282.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
    AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
    CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
    GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
    GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
    ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
    AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
    CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
    GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
    GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
    GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
    AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
    TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
    ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
    GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
    GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
    GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
    TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
    GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
    AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
    CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
    GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
    CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
    GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
    CATCCAAACT GCACCTACGG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25365
    Clone ID Related Accession (Same CDS sequence) NM_201282.1 , NM_201282.2
    Accession Version NM_201282.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1887bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product epidermal growth factor receptor isoform b precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006977.3, AW163038.1, X00588.1, AF125253.1 and AF277897.1. On May 31, 2019 this sequence version replaced NM_201282.1. Transcript Variant: This variant (2) uses a different 3' terminal exon when compared to variant 1. The resulting isoform (b) has a shorter and distinct C-terminus. Only the extracellular domain is present in isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 11161793 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
    AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
    CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
    GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
    GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
    ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
    AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
    CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
    GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
    GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
    GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
    AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
    TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
    ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
    GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
    GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
    GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
    TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
    GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
    AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
    CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
    GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
    CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
    GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
    CATCCAAACT GCACCTACGG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_958439.1
    CDS262..2148
    Translation

    Target ORF information:

    RefSeq Version NM_201282.2
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_201282.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
    AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
    CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
    GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
    GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
    ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
    AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
    CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
    GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
    GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
    GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
    AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
    TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
    ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
    GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
    GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
    GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
    TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
    GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
    AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
    CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
    GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
    CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
    GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
    CATCCAAACT GCACCTACGG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25409
    Clone ID Related Accession (Same CDS sequence) NM_201283.1
    Accession Version NM_201283.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1218bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-25
    Organism Homo sapiens(human)
    Product epidermal growth factor receptor isoform c precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AW163038.1, X00588.1 and U48722.1. Transcript Variant: This variant (3, also known as ErbB1-S) uses a different 3' terminal exon when compared to variant 1. The resulting isoform (c) has a shorter and distinct C-terminus. Only the extracellular domain is present in isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U48722.1, AY698024.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
    AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
    CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
    GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
    GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
    ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
    AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
    CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
    GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
    GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
    GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
    AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
    TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
    ATCACAGGTT TGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_958440.1
    CDS247..1464
    Translation

    Target ORF information:

    RefSeq Version NM_201283.1
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_201283.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
    AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
    CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
    GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
    GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
    ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
    AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
    CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
    GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
    GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
    GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
    AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
    TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
    ATCACAGGTT TGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25437
    Clone ID Related Accession (Same CDS sequence) NM_005228.3
    Accession Version NM_005228.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-14
    Organism Homo sapiens(Human)
    Product epidermal growth factor receptor isoform a precursor
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
    AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
    CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
    GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
    GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
    ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
    AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
    CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
    GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
    GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
    GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
    AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
    TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
    ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
    GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
    GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
    GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
    TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
    GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
    AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
    CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
    GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
    CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
    GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
    CATCCAAACT GCACCTACGG ATGCACTGGG CCAGGTCTTG AAGGCTGTCC AACGAATGGG
    CCTAAGATCC CGTCCATCGC CACTGGGATG GTGGGGGCCC TCCTCTTGCT GCTGGTGGTG
    GCCCTGGGGA TCGGCCTCTT CATGCGAAGG CGCCACATCG TTCGGAAGCG CACGCTGCGG
    AGGCTGCTGC AGGAGAGGGA GCTTGTGGAG CCTCTTACAC CCAGTGGAGA AGCTCCCAAC
    CAAGCTCTCT TGAGGATCTT GAAGGAAACT GAATTCAAAA AGATCAAAGT GCTGGGCTCC
    GGTGCGTTCG GCACGGTGTA TAAGGGACTC TGGATCCCAG AAGGTGAGAA AGTTAAAATT
    CCCGTCGCTA TCAAGGAATT AAGAGAAGCA ACATCTCCGA AAGCCAACAA GGAAATCCTC
    GATGAAGCCT ACGTGATGGC CAGCGTGGAC AACCCCCACG TGTGCCGCCT GCTGGGCATC
    TGCCTCACCT CCACCGTGCA GCTCATCACG CAGCTCATGC CCTTCGGCTG CCTCCTGGAC
    TATGTCCGGG AACACAAAGA CAATATTGGC TCCCAGTACC TGCTCAACTG GTGTGTGCAG
    ATCGCAAAGG GCATGAACTA CTTGGAGGAC CGTCGCTTGG TGCACCGCGA CCTGGCAGCC
    AGGAACGTAC TGGTGAAAAC ACCGCAGCAT GTCAAGATCA CAGATTTTGG GCTGGCCAAA
    CTGCTGGGTG CGGAAGAGAA AGAATACCAT GCAGAAGGAG GCAAAGTGCC TATCAAGTGG
    ATGGCATTGG AATCAATTTT ACACAGAATC TATACCCACC AGAGTGATGT CTGGAGCTAC
    GGGGTGACCG TTTGGGAGTT GATGACCTTT GGATCCAAGC CATATGACGG AATCCCTGCC
    AGCGAGATCT CCTCCATCCT GGAGAAAGGA GAACGCCTCC CTCAGCCACC CATATGTACC
    ATCGATGTCT ACATGATCAT GGTCAAGTGC TGGATGATAG ACGCAGATAG TCGCCCAAAG
    TTCCGTGAGT TGATCATCGA ATTCTCCAAA ATGGCCCGAG ACCCCCAGCG CTACCTTGTC
    ATTCAGGGGG ATGAAAGAAT GCATTTGCCA AGTCCTACAG ACTCCAACTT CTACCGTGCC
    CTGATGGATG AAGAAGACAT GGACGACGTG GTGGATGCCG ACGAGTACCT CATCCCACAG
    CAGGGCTTCT TCAGCAGCCC CTCCACGTCA CGGACTCCCC TCCTGAGCTC TCTGAGTGCA
    ACCAGCAACA ATTCCACCGT GGCTTGCATT GATAGAAATG GGCTGCAAAG CTGTCCCATC
    AAGGAAGACA GCTTCTTGCA GCGATACAGC TCAGACCCCA CAGGCGCCTT GACTGAGGAC
    AGCATAGACG ACACCTTCCT CCCAGTGCCT GAATACATAA ACCAGTCCGT TCCCAAAAGG
    CCCGCTGGCT CTGTGCAGAA TCCTGTCTAT CACAATCAGC CTCTGAACCC CGCGCCCAGC
    AGAGACCCAC ACTACCAGGA CCCCCACAGC ACTGCAGTGG GCAACCCCGA GTATCTCAAC
    ACTGTCCAGC CCACCTGTGT CAACAGCACA TTCGACAGCC CTGCCCACTG GGCCCAGAAA
    GGCAGCCACC AAATTAGCCT GGACAACCCT GACTACCAGC AGGACTTCTT TCCCAAGGAA
    GCCAAGCCAA ATGGCATCTT TAAGGGCTCC ACAGCTGAAA ATGCAGAATA CCTAAGGGTC
    GCGCCACAAA GCAGTGAATT TATTGGAGCA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005219.2
    Translation

    Target ORF information:

    RefSeq Version NM_005228.3
    Organism Homo sapiens(Human)
    Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005228.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
    AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
    CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
    GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
    GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
    ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
    AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
    CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
    GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
    GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
    GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
    AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
    TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
    ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
    GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
    GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
    GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
    TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
    GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
    AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
    CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
    GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
    CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
    GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
    CATCCAAACT GCACCTACGG ATGCACTGGG CCAGGTCTTG AAGGCTGTCC AACGAATGGG
    CCTAAGATCC CGTCCATCGC CACTGGGATG GTGGGGGCCC TCCTCTTGCT GCTGGTGGTG
    GCCCTGGGGA TCGGCCTCTT CATGCGAAGG CGCCACATCG TTCGGAAGCG CACGCTGCGG
    AGGCTGCTGC AGGAGAGGGA GCTTGTGGAG CCTCTTACAC CCAGTGGAGA AGCTCCCAAC
    CAAGCTCTCT TGAGGATCTT GAAGGAAACT GAATTCAAAA AGATCAAAGT GCTGGGCTCC
    GGTGCGTTCG GCACGGTGTA TAAGGGACTC TGGATCCCAG AAGGTGAGAA AGTTAAAATT
    CCCGTCGCTA TCAAGGAATT AAGAGAAGCA ACATCTCCGA AAGCCAACAA GGAAATCCTC
    GATGAAGCCT ACGTGATGGC CAGCGTGGAC AACCCCCACG TGTGCCGCCT GCTGGGCATC
    TGCCTCACCT CCACCGTGCA GCTCATCACG CAGCTCATGC CCTTCGGCTG CCTCCTGGAC
    TATGTCCGGG AACACAAAGA CAATATTGGC TCCCAGTACC TGCTCAACTG GTGTGTGCAG
    ATCGCAAAGG GCATGAACTA CTTGGAGGAC CGTCGCTTGG TGCACCGCGA CCTGGCAGCC
    AGGAACGTAC TGGTGAAAAC ACCGCAGCAT GTCAAGATCA CAGATTTTGG GCTGGCCAAA
    CTGCTGGGTG CGGAAGAGAA AGAATACCAT GCAGAAGGAG GCAAAGTGCC TATCAAGTGG
    ATGGCATTGG AATCAATTTT ACACAGAATC TATACCCACC AGAGTGATGT CTGGAGCTAC
    GGGGTGACCG TTTGGGAGTT GATGACCTTT GGATCCAAGC CATATGACGG AATCCCTGCC
    AGCGAGATCT CCTCCATCCT GGAGAAAGGA GAACGCCTCC CTCAGCCACC CATATGTACC
    ATCGATGTCT ACATGATCAT GGTCAAGTGC TGGATGATAG ACGCAGATAG TCGCCCAAAG
    TTCCGTGAGT TGATCATCGA ATTCTCCAAA ATGGCCCGAG ACCCCCAGCG CTACCTTGTC
    ATTCAGGGGG ATGAAAGAAT GCATTTGCCA AGTCCTACAG ACTCCAACTT CTACCGTGCC
    CTGATGGATG AAGAAGACAT GGACGACGTG GTGGATGCCG ACGAGTACCT CATCCCACAG
    CAGGGCTTCT TCAGCAGCCC CTCCACGTCA CGGACTCCCC TCCTGAGCTC TCTGAGTGCA
    ACCAGCAACA ATTCCACCGT GGCTTGCATT GATAGAAATG GGCTGCAAAG CTGTCCCATC
    AAGGAAGACA GCTTCTTGCA GCGATACAGC TCAGACCCCA CAGGCGCCTT GACTGAGGAC
    AGCATAGACG ACACCTTCCT CCCAGTGCCT GAATACATAA ACCAGTCCGT TCCCAAAAGG
    CCCGCTGGCT CTGTGCAGAA TCCTGTCTAT CACAATCAGC CTCTGAACCC CGCGCCCAGC
    AGAGACCCAC ACTACCAGGA CCCCCACAGC ACTGCAGTGG GCAACCCCGA GTATCTCAAC
    ACTGTCCAGC CCACCTGTGT CAACAGCACA TTCGACAGCC CTGCCCACTG GGCCCAGAAA
    GGCAGCCACC AAATTAGCCT GGACAACCCT GACTACCAGC AGGACTTCTT TCCCAAGGAA
    GCCAAGCCAA ATGGCATCTT TAAGGGCTCC ACAGCTGAAA ATGCAGAATA CCTAAGGGTC
    GCGCCACAAA GCAGTGAATT TATTGGAGCA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25398
    Clone ID Related Accession (Same CDS sequence) NM_201284.2 , NM_201284.1
    Accession Version NM_201284.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2118bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-01
    Organism Homo sapiens(human)
    Product epidermal growth factor receptor isoform d precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006977.3, AW163038.1, X00588.1 and AF125253.1. On May 31, 2019 this sequence version replaced NM_201284.1. Transcript Variant: This variant (4) uses a different 3' terminal exon when compared to variant 1. The resulting isoform (d) has a shorter and distinct C-terminus. Only the extracellular domain is present in isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF125253.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149398, SAMN03465410 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
    AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
    CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
    GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
    GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
    ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
    AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
    CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
    GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
    GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
    GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
    AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
    TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
    ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
    GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
    GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
    GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
    TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
    GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
    AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
    CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
    GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
    CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
    GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
    CATCCAAACT GCACCTACGG GCCAGGAAAT GAGAGTCTCA AAGCCATGTT ATTCTGCCTT
    TTTAAACTAT CATCCTGTAA TCAAAGTAAT GATGGCAGCG TGTCCCACCA GAGCGGGAGC
    CCAGCTGCTC AGGAGTCATG CTTAGGATGG ATCCCTTCTC TTCTGCCGTC AGAGTTTCAG
    CTGGGTTGGG GTGGATGCAG CCACCTCCAT GCCTGGCCTT CTGCATCTGT GATCATCACG
    GCCTCCTCCT GCCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_958441.1
    CDS262..2379
    Translation

    Target ORF information:

    RefSeq Version NM_201284.2
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_201284.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
    AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
    CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
    GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
    GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
    ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
    AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
    CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
    GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
    GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
    GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
    AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
    TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
    ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
    GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
    GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
    GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
    TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
    GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
    AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
    CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
    GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
    CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
    GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
    CATCCAAACT GCACCTACGG GCCAGGAAAT GAGAGTCTCA AAGCCATGTT ATTCTGCCTT
    TTTAAACTAT CATCCTGTAA TCAAAGTAAT GATGGCAGCG TGTCCCACCA GAGCGGGAGC
    CCAGCTGCTC AGGAGTCATG CTTAGGATGG ATCCCTTCTC TTCTGCCGTC AGAGTTTCAG
    CTGGGTTGGG GTGGATGCAG CCACCTCCAT GCCTGGCCTT CTGCATCTGT GATCATCACG
    GCCTCCTCCT GCCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25398
    Clone ID Related Accession (Same CDS sequence) NM_201284.2 , NM_201284.1
    Accession Version NM_201284.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2118bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product epidermal growth factor receptor isoform d precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AW163038.1, X00588.1 and AF125253.1. Transcript Variant: This variant (4) uses a different 3' terminal exon when compared to variant 1. The resulting isoform (d) has a shorter and distinct C-terminus. Only the extracellular domain is present in isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF125253.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149398, SAMN03465410 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
    AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
    CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
    GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
    GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
    ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
    AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
    CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
    GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
    GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
    GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
    AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
    TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
    ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
    GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
    GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
    GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
    TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
    GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
    AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
    CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
    GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
    CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
    GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
    CATCCAAACT GCACCTACGG GCCAGGAAAT GAGAGTCTCA AAGCCATGTT ATTCTGCCTT
    TTTAAACTAT CATCCTGTAA TCAAAGTAAT GATGGCAGCG TGTCCCACCA GAGCGGGAGC
    CCAGCTGCTC AGGAGTCATG CTTAGGATGG ATCCCTTCTC TTCTGCCGTC AGAGTTTCAG
    CTGGGTTGGG GTGGATGCAG CCACCTCCAT GCCTGGCCTT CTGCATCTGT GATCATCACG
    GCCTCCTCCT GCCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_958441.1
    CDS247..2364
    Translation

    Target ORF information:

    RefSeq Version NM_201284.1
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_201284.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
    AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
    CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
    GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
    GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
    ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
    AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
    CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
    GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
    GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
    GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
    AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
    TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
    ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
    GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
    GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
    GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
    TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
    GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
    AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
    CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
    GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
    CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
    GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
    CATCCAAACT GCACCTACGG GCCAGGAAAT GAGAGTCTCA AAGCCATGTT ATTCTGCCTT
    TTTAAACTAT CATCCTGTAA TCAAAGTAAT GATGGCAGCG TGTCCCACCA GAGCGGGAGC
    CCAGCTGCTC AGGAGTCATG CTTAGGATGG ATCCCTTCTC TTCTGCCGTC AGAGTTTCAG
    CTGGGTTGGG GTGGATGCAG CCACCTCCAT GCCTGGCCTT CTGCATCTGT GATCATCACG
    GCCTCCTCCT GCCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102763
    Clone ID Related Accession (Same CDS sequence) NM_001346941.2 , NM_001346941.1
    Accession Version NM_001346941.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2832bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-15
    Organism Homo sapiens(human)
    Product epidermal growth factor receptor isoform i precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006977.3 and AC073324.6. On May 31, 2019 this sequence version replaced NM_001346941.1. Transcript Variant: This variant (EGFRvIII, also known as delta-EGFR and de2-7EGFR) has an in-frame deletion of six exons in the 5' coding region, compared to variant 1. The encoded isoform (i) has a shorter extracellular domain compared to isoform a. This variant is considered to be tumorigenic and the encoded protein lacks normal ligand binding ability and is constitutively active (PMID: 23777544). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## inferred exon combination :: PMID: 23777544 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGGT AATTATGTGG TGACAGATCA CGGCTCGTGC
    GTCCGAGCCT GTGGGGCCGA CAGCTATGAG ATGGAGGAAG ACGGCGTCCG CAAGTGTAAG
    AAGTGCGAAG GGCCTTGCCG CAAAGTGTGT AACGGAATAG GTATTGGTGA ATTTAAAGAC
    TCACTCTCCA TAAATGCTAC GAATATTAAA CACTTCAAAA ACTGCACCTC CATCAGTGGC
    GATCTCCACA TCCTGCCGGT GGCATTTAGG GGTGACTCCT TCACACATAC TCCTCCTCTG
    GATCCACAGG AACTGGATAT TCTGAAAACC GTAAAGGAAA TCACAGGGTT TTTGCTGATT
    CAGGCTTGGC CTGAAAACAG GACGGACCTC CATGCCTTTG AGAACCTAGA AATCATACGC
    GGCAGGACCA AGCAACATGG TCAGTTTTCT CTTGCAGTCG TCAGCCTGAA CATAACATCC
    TTGGGATTAC GCTCCCTCAA GGAGATAAGT GATGGAGATG TGATAATTTC AGGAAACAAA
    AATTTGTGCT ATGCAAATAC AATAAACTGG AAAAAACTGT TTGGGACCTC CGGTCAGAAA
    ACCAAAATTA TAAGCAACAG AGGTGAAAAC AGCTGCAAGG CCACAGGCCA GGTCTGCCAT
    GCCTTGTGCT CCCCCGAGGG CTGCTGGGGC CCGGAGCCCA GGGACTGCGT CTCTTGCCGG
    AATGTCAGCC GAGGCAGGGA ATGCGTGGAC AAGTGCAACC TTCTGGAGGG TGAGCCAAGG
    GAGTTTGTGG AGAACTCTGA GTGCATACAG TGCCACCCAG AGTGCCTGCC TCAGGCCATG
    AACATCACCT GCACAGGACG GGGACCAGAC AACTGTATCC AGTGTGCCCA CTACATTGAC
    GGCCCCCACT GCGTCAAGAC CTGCCCGGCA GGAGTCATGG GAGAAAACAA CACCCTGGTC
    TGGAAGTACG CAGACGCCGG CCATGTGTGC CACCTGTGCC ATCCAAACTG CACCTACGGA
    TGCACTGGGC CAGGTCTTGA AGGCTGTCCA ACGAATGGGC CTAAGATCCC GTCCATCGCC
    ACTGGGATGG TGGGGGCCCT CCTCTTGCTG CTGGTGGTGG CCCTGGGGAT CGGCCTCTTC
    ATGCGAAGGC GCCACATCGT TCGGAAGCGC ACGCTGCGGA GGCTGCTGCA GGAGAGGGAG
    CTTGTGGAGC CTCTTACACC CAGTGGAGAA GCTCCCAACC AAGCTCTCTT GAGGATCTTG
    AAGGAAACTG AATTCAAAAA GATCAAAGTG CTGGGCTCCG GTGCGTTCGG CACGGTGTAT
    AAGGGACTCT GGATCCCAGA AGGTGAGAAA GTTAAAATTC CCGTCGCTAT CAAGGAATTA
    AGAGAAGCAA CATCTCCGAA AGCCAACAAG GAAATCCTCG ATGAAGCCTA CGTGATGGCC
    AGCGTGGACA ACCCCCACGT GTGCCGCCTG CTGGGCATCT GCCTCACCTC CACCGTGCAG
    CTCATCACGC AGCTCATGCC CTTCGGCTGC CTCCTGGACT ATGTCCGGGA ACACAAAGAC
    AATATTGGCT CCCAGTACCT GCTCAACTGG TGTGTGCAGA TCGCAAAGGG CATGAACTAC
    TTGGAGGACC GTCGCTTGGT GCACCGCGAC CTGGCAGCCA GGAACGTACT GGTGAAAACA
    CCGCAGCATG TCAAGATCAC AGATTTTGGG CTGGCCAAAC TGCTGGGTGC GGAAGAGAAA
    GAATACCATG CAGAAGGAGG CAAAGTGCCT ATCAAGTGGA TGGCATTGGA ATCAATTTTA
    CACAGAATCT ATACCCACCA GAGTGATGTC TGGAGCTACG GGGTGACTGT TTGGGAGTTG
    ATGACCTTTG GATCCAAGCC ATATGACGGA ATCCCTGCCA GCGAGATCTC CTCCATCCTG
    GAGAAAGGAG AACGCCTCCC TCAGCCACCC ATATGTACCA TCGATGTCTA CATGATCATG
    GTCAAGTGCT GGATGATAGA CGCAGATAGT CGCCCAAAGT TCCGTGAGTT GATCATCGAA
    TTCTCCAAAA TGGCCCGAGA CCCCCAGCGC TACCTTGTCA TTCAGGGGGA TGAAAGAATG
    CATTTGCCAA GTCCTACAGA CTCCAACTTC TACCGTGCCC TGATGGATGA AGAAGACATG
    GACGACGTGG TGGATGCCGA CGAGTACCTC ATCCCACAGC AGGGCTTCTT CAGCAGCCCC
    TCCACGTCAC GGACTCCCCT CCTGAGCTCT CTGAGTGCAA CCAGCAACAA TTCCACCGTG
    GCTTGCATTG ATAGAAATGG GCTGCAAAGC TGTCCCATCA AGGAAGACAG CTTCTTGCAG
    CGATACAGCT CAGACCCCAC AGGCGCCTTG ACTGAGGACA GCATAGACGA CACCTTCCTC
    CCAGTGCCTG AATACATAAA CCAGTCCGTT CCCAAAAGGC CCGCTGGCTC TGTGCAGAAT
    CCTGTCTATC ACAATCAGCC TCTGAACCCC GCGCCCAGCA GAGACCCACA CTACCAGGAC
    CCCCACAGCA CTGCAGTGGG CAACCCCGAG TATCTCAACA CTGTCCAGCC CACCTGTGTC
    AACAGCACAT TCGACAGCCC TGCCCACTGG GCCCAGAAAG GCAGCCACCA AATTAGCCTG
    GACAACCCTG ACTACCAGCA GGACTTCTTT CCCAAGGAAG CCAAGCCAAA TGGCATCTTT
    AAGGGCTCCA CAGCTGAAAA TGCAGAATAC CTAAGGGTCG CGCCACAAAG CAGTGAATTT
    ATTGGAGCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333870.1
    CDS262..3093
    Translation

    Target ORF information:

    RefSeq Version NM_001346941.2
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant EGFRvIII, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346941.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGGT AATTATGTGG TGACAGATCA CGGCTCGTGC
    GTCCGAGCCT GTGGGGCCGA CAGCTATGAG ATGGAGGAAG ACGGCGTCCG CAAGTGTAAG
    AAGTGCGAAG GGCCTTGCCG CAAAGTGTGT AACGGAATAG GTATTGGTGA ATTTAAAGAC
    TCACTCTCCA TAAATGCTAC GAATATTAAA CACTTCAAAA ACTGCACCTC CATCAGTGGC
    GATCTCCACA TCCTGCCGGT GGCATTTAGG GGTGACTCCT TCACACATAC TCCTCCTCTG
    GATCCACAGG AACTGGATAT TCTGAAAACC GTAAAGGAAA TCACAGGGTT TTTGCTGATT
    CAGGCTTGGC CTGAAAACAG GACGGACCTC CATGCCTTTG AGAACCTAGA AATCATACGC
    GGCAGGACCA AGCAACATGG TCAGTTTTCT CTTGCAGTCG TCAGCCTGAA CATAACATCC
    TTGGGATTAC GCTCCCTCAA GGAGATAAGT GATGGAGATG TGATAATTTC AGGAAACAAA
    AATTTGTGCT ATGCAAATAC AATAAACTGG AAAAAACTGT TTGGGACCTC CGGTCAGAAA
    ACCAAAATTA TAAGCAACAG AGGTGAAAAC AGCTGCAAGG CCACAGGCCA GGTCTGCCAT
    GCCTTGTGCT CCCCCGAGGG CTGCTGGGGC CCGGAGCCCA GGGACTGCGT CTCTTGCCGG
    AATGTCAGCC GAGGCAGGGA ATGCGTGGAC AAGTGCAACC TTCTGGAGGG TGAGCCAAGG
    GAGTTTGTGG AGAACTCTGA GTGCATACAG TGCCACCCAG AGTGCCTGCC TCAGGCCATG
    AACATCACCT GCACAGGACG GGGACCAGAC AACTGTATCC AGTGTGCCCA CTACATTGAC
    GGCCCCCACT GCGTCAAGAC CTGCCCGGCA GGAGTCATGG GAGAAAACAA CACCCTGGTC
    TGGAAGTACG CAGACGCCGG CCATGTGTGC CACCTGTGCC ATCCAAACTG CACCTACGGA
    TGCACTGGGC CAGGTCTTGA AGGCTGTCCA ACGAATGGGC CTAAGATCCC GTCCATCGCC
    ACTGGGATGG TGGGGGCCCT CCTCTTGCTG CTGGTGGTGG CCCTGGGGAT CGGCCTCTTC
    ATGCGAAGGC GCCACATCGT TCGGAAGCGC ACGCTGCGGA GGCTGCTGCA GGAGAGGGAG
    CTTGTGGAGC CTCTTACACC CAGTGGAGAA GCTCCCAACC AAGCTCTCTT GAGGATCTTG
    AAGGAAACTG AATTCAAAAA GATCAAAGTG CTGGGCTCCG GTGCGTTCGG CACGGTGTAT
    AAGGGACTCT GGATCCCAGA AGGTGAGAAA GTTAAAATTC CCGTCGCTAT CAAGGAATTA
    AGAGAAGCAA CATCTCCGAA AGCCAACAAG GAAATCCTCG ATGAAGCCTA CGTGATGGCC
    AGCGTGGACA ACCCCCACGT GTGCCGCCTG CTGGGCATCT GCCTCACCTC CACCGTGCAG
    CTCATCACGC AGCTCATGCC CTTCGGCTGC CTCCTGGACT ATGTCCGGGA ACACAAAGAC
    AATATTGGCT CCCAGTACCT GCTCAACTGG TGTGTGCAGA TCGCAAAGGG CATGAACTAC
    TTGGAGGACC GTCGCTTGGT GCACCGCGAC CTGGCAGCCA GGAACGTACT GGTGAAAACA
    CCGCAGCATG TCAAGATCAC AGATTTTGGG CTGGCCAAAC TGCTGGGTGC GGAAGAGAAA
    GAATACCATG CAGAAGGAGG CAAAGTGCCT ATCAAGTGGA TGGCATTGGA ATCAATTTTA
    CACAGAATCT ATACCCACCA GAGTGATGTC TGGAGCTACG GGGTGACTGT TTGGGAGTTG
    ATGACCTTTG GATCCAAGCC ATATGACGGA ATCCCTGCCA GCGAGATCTC CTCCATCCTG
    GAGAAAGGAG AACGCCTCCC TCAGCCACCC ATATGTACCA TCGATGTCTA CATGATCATG
    GTCAAGTGCT GGATGATAGA CGCAGATAGT CGCCCAAAGT TCCGTGAGTT GATCATCGAA
    TTCTCCAAAA TGGCCCGAGA CCCCCAGCGC TACCTTGTCA TTCAGGGGGA TGAAAGAATG
    CATTTGCCAA GTCCTACAGA CTCCAACTTC TACCGTGCCC TGATGGATGA AGAAGACATG
    GACGACGTGG TGGATGCCGA CGAGTACCTC ATCCCACAGC AGGGCTTCTT CAGCAGCCCC
    TCCACGTCAC GGACTCCCCT CCTGAGCTCT CTGAGTGCAA CCAGCAACAA TTCCACCGTG
    GCTTGCATTG ATAGAAATGG GCTGCAAAGC TGTCCCATCA AGGAAGACAG CTTCTTGCAG
    CGATACAGCT CAGACCCCAC AGGCGCCTTG ACTGAGGACA GCATAGACGA CACCTTCCTC
    CCAGTGCCTG AATACATAAA CCAGTCCGTT CCCAAAAGGC CCGCTGGCTC TGTGCAGAAT
    CCTGTCTATC ACAATCAGCC TCTGAACCCC GCGCCCAGCA GAGACCCACA CTACCAGGAC
    CCCCACAGCA CTGCAGTGGG CAACCCCGAG TATCTCAACA CTGTCCAGCC CACCTGTGTC
    AACAGCACAT TCGACAGCCC TGCCCACTGG GCCCAGAAAG GCAGCCACCA AATTAGCCTG
    GACAACCCTG ACTACCAGCA GGACTTCTTT CCCAAGGAAG CCAAGCCAAA TGGCATCTTT
    AAGGGCTCCA CAGCTGAAAA TGCAGAATAC CTAAGGGTCG CGCCACAAAG CAGTGAATTT
    ATTGGAGCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102763
    Clone ID Related Accession (Same CDS sequence) NM_001346941.2 , NM_001346941.1
    Accession Version NM_001346941.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2832bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product epidermal growth factor receptor isoform i precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006977.3 and AC073324.6. Transcript Variant: This variant (EGFRvIII, also known as delta-EGFR and de2-7EGFR) has an in-frame deletion of six exons in the 5' coding region, compared to variant 1. The encoded isoform (i) has a shorter extracellular domain compared to isoform a. This variant is considered to be tumorigenic and the encoded protein lacks normal ligand binding ability and is constitutively active (PMID: 23777544). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## inferred exon combination :: PMID: 23777544 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGGT AATTATGTGG TGACAGATCA CGGCTCGTGC
    GTCCGAGCCT GTGGGGCCGA CAGCTATGAG ATGGAGGAAG ACGGCGTCCG CAAGTGTAAG
    AAGTGCGAAG GGCCTTGCCG CAAAGTGTGT AACGGAATAG GTATTGGTGA ATTTAAAGAC
    TCACTCTCCA TAAATGCTAC GAATATTAAA CACTTCAAAA ACTGCACCTC CATCAGTGGC
    GATCTCCACA TCCTGCCGGT GGCATTTAGG GGTGACTCCT TCACACATAC TCCTCCTCTG
    GATCCACAGG AACTGGATAT TCTGAAAACC GTAAAGGAAA TCACAGGGTT TTTGCTGATT
    CAGGCTTGGC CTGAAAACAG GACGGACCTC CATGCCTTTG AGAACCTAGA AATCATACGC
    GGCAGGACCA AGCAACATGG TCAGTTTTCT CTTGCAGTCG TCAGCCTGAA CATAACATCC
    TTGGGATTAC GCTCCCTCAA GGAGATAAGT GATGGAGATG TGATAATTTC AGGAAACAAA
    AATTTGTGCT ATGCAAATAC AATAAACTGG AAAAAACTGT TTGGGACCTC CGGTCAGAAA
    ACCAAAATTA TAAGCAACAG AGGTGAAAAC AGCTGCAAGG CCACAGGCCA GGTCTGCCAT
    GCCTTGTGCT CCCCCGAGGG CTGCTGGGGC CCGGAGCCCA GGGACTGCGT CTCTTGCCGG
    AATGTCAGCC GAGGCAGGGA ATGCGTGGAC AAGTGCAACC TTCTGGAGGG TGAGCCAAGG
    GAGTTTGTGG AGAACTCTGA GTGCATACAG TGCCACCCAG AGTGCCTGCC TCAGGCCATG
    AACATCACCT GCACAGGACG GGGACCAGAC AACTGTATCC AGTGTGCCCA CTACATTGAC
    GGCCCCCACT GCGTCAAGAC CTGCCCGGCA GGAGTCATGG GAGAAAACAA CACCCTGGTC
    TGGAAGTACG CAGACGCCGG CCATGTGTGC CACCTGTGCC ATCCAAACTG CACCTACGGA
    TGCACTGGGC CAGGTCTTGA AGGCTGTCCA ACGAATGGGC CTAAGATCCC GTCCATCGCC
    ACTGGGATGG TGGGGGCCCT CCTCTTGCTG CTGGTGGTGG CCCTGGGGAT CGGCCTCTTC
    ATGCGAAGGC GCCACATCGT TCGGAAGCGC ACGCTGCGGA GGCTGCTGCA GGAGAGGGAG
    CTTGTGGAGC CTCTTACACC CAGTGGAGAA GCTCCCAACC AAGCTCTCTT GAGGATCTTG
    AAGGAAACTG AATTCAAAAA GATCAAAGTG CTGGGCTCCG GTGCGTTCGG CACGGTGTAT
    AAGGGACTCT GGATCCCAGA AGGTGAGAAA GTTAAAATTC CCGTCGCTAT CAAGGAATTA
    AGAGAAGCAA CATCTCCGAA AGCCAACAAG GAAATCCTCG ATGAAGCCTA CGTGATGGCC
    AGCGTGGACA ACCCCCACGT GTGCCGCCTG CTGGGCATCT GCCTCACCTC CACCGTGCAG
    CTCATCACGC AGCTCATGCC CTTCGGCTGC CTCCTGGACT ATGTCCGGGA ACACAAAGAC
    AATATTGGCT CCCAGTACCT GCTCAACTGG TGTGTGCAGA TCGCAAAGGG CATGAACTAC
    TTGGAGGACC GTCGCTTGGT GCACCGCGAC CTGGCAGCCA GGAACGTACT GGTGAAAACA
    CCGCAGCATG TCAAGATCAC AGATTTTGGG CTGGCCAAAC TGCTGGGTGC GGAAGAGAAA
    GAATACCATG CAGAAGGAGG CAAAGTGCCT ATCAAGTGGA TGGCATTGGA ATCAATTTTA
    CACAGAATCT ATACCCACCA GAGTGATGTC TGGAGCTACG GGGTGACTGT TTGGGAGTTG
    ATGACCTTTG GATCCAAGCC ATATGACGGA ATCCCTGCCA GCGAGATCTC CTCCATCCTG
    GAGAAAGGAG AACGCCTCCC TCAGCCACCC ATATGTACCA TCGATGTCTA CATGATCATG
    GTCAAGTGCT GGATGATAGA CGCAGATAGT CGCCCAAAGT TCCGTGAGTT GATCATCGAA
    TTCTCCAAAA TGGCCCGAGA CCCCCAGCGC TACCTTGTCA TTCAGGGGGA TGAAAGAATG
    CATTTGCCAA GTCCTACAGA CTCCAACTTC TACCGTGCCC TGATGGATGA AGAAGACATG
    GACGACGTGG TGGATGCCGA CGAGTACCTC ATCCCACAGC AGGGCTTCTT CAGCAGCCCC
    TCCACGTCAC GGACTCCCCT CCTGAGCTCT CTGAGTGCAA CCAGCAACAA TTCCACCGTG
    GCTTGCATTG ATAGAAATGG GCTGCAAAGC TGTCCCATCA AGGAAGACAG CTTCTTGCAG
    CGATACAGCT CAGACCCCAC AGGCGCCTTG ACTGAGGACA GCATAGACGA CACCTTCCTC
    CCAGTGCCTG AATACATAAA CCAGTCCGTT CCCAAAAGGC CCGCTGGCTC TGTGCAGAAT
    CCTGTCTATC ACAATCAGCC TCTGAACCCC GCGCCCAGCA GAGACCCACA CTACCAGGAC
    CCCCACAGCA CTGCAGTGGG CAACCCCGAG TATCTCAACA CTGTCCAGCC CACCTGTGTC
    AACAGCACAT TCGACAGCCC TGCCCACTGG GCCCAGAAAG GCAGCCACCA AATTAGCCTG
    GACAACCCTG ACTACCAGCA GGACTTCTTT CCCAAGGAAG CCAAGCCAAA TGGCATCTTT
    AAGGGCTCCA CAGCTGAAAA TGCAGAATAC CTAAGGGTCG CGCCACAAAG CAGTGAATTT
    ATTGGAGCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333870.1
    CDS258..3089
    Translation

    Target ORF information:

    RefSeq Version NM_001346941.1
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant EGFRvIII, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346941.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGGT AATTATGTGG TGACAGATCA CGGCTCGTGC
    GTCCGAGCCT GTGGGGCCGA CAGCTATGAG ATGGAGGAAG ACGGCGTCCG CAAGTGTAAG
    AAGTGCGAAG GGCCTTGCCG CAAAGTGTGT AACGGAATAG GTATTGGTGA ATTTAAAGAC
    TCACTCTCCA TAAATGCTAC GAATATTAAA CACTTCAAAA ACTGCACCTC CATCAGTGGC
    GATCTCCACA TCCTGCCGGT GGCATTTAGG GGTGACTCCT TCACACATAC TCCTCCTCTG
    GATCCACAGG AACTGGATAT TCTGAAAACC GTAAAGGAAA TCACAGGGTT TTTGCTGATT
    CAGGCTTGGC CTGAAAACAG GACGGACCTC CATGCCTTTG AGAACCTAGA AATCATACGC
    GGCAGGACCA AGCAACATGG TCAGTTTTCT CTTGCAGTCG TCAGCCTGAA CATAACATCC
    TTGGGATTAC GCTCCCTCAA GGAGATAAGT GATGGAGATG TGATAATTTC AGGAAACAAA
    AATTTGTGCT ATGCAAATAC AATAAACTGG AAAAAACTGT TTGGGACCTC CGGTCAGAAA
    ACCAAAATTA TAAGCAACAG AGGTGAAAAC AGCTGCAAGG CCACAGGCCA GGTCTGCCAT
    GCCTTGTGCT CCCCCGAGGG CTGCTGGGGC CCGGAGCCCA GGGACTGCGT CTCTTGCCGG
    AATGTCAGCC GAGGCAGGGA ATGCGTGGAC AAGTGCAACC TTCTGGAGGG TGAGCCAAGG
    GAGTTTGTGG AGAACTCTGA GTGCATACAG TGCCACCCAG AGTGCCTGCC TCAGGCCATG
    AACATCACCT GCACAGGACG GGGACCAGAC AACTGTATCC AGTGTGCCCA CTACATTGAC
    GGCCCCCACT GCGTCAAGAC CTGCCCGGCA GGAGTCATGG GAGAAAACAA CACCCTGGTC
    TGGAAGTACG CAGACGCCGG CCATGTGTGC CACCTGTGCC ATCCAAACTG CACCTACGGA
    TGCACTGGGC CAGGTCTTGA AGGCTGTCCA ACGAATGGGC CTAAGATCCC GTCCATCGCC
    ACTGGGATGG TGGGGGCCCT CCTCTTGCTG CTGGTGGTGG CCCTGGGGAT CGGCCTCTTC
    ATGCGAAGGC GCCACATCGT TCGGAAGCGC ACGCTGCGGA GGCTGCTGCA GGAGAGGGAG
    CTTGTGGAGC CTCTTACACC CAGTGGAGAA GCTCCCAACC AAGCTCTCTT GAGGATCTTG
    AAGGAAACTG AATTCAAAAA GATCAAAGTG CTGGGCTCCG GTGCGTTCGG CACGGTGTAT
    AAGGGACTCT GGATCCCAGA AGGTGAGAAA GTTAAAATTC CCGTCGCTAT CAAGGAATTA
    AGAGAAGCAA CATCTCCGAA AGCCAACAAG GAAATCCTCG ATGAAGCCTA CGTGATGGCC
    AGCGTGGACA ACCCCCACGT GTGCCGCCTG CTGGGCATCT GCCTCACCTC CACCGTGCAG
    CTCATCACGC AGCTCATGCC CTTCGGCTGC CTCCTGGACT ATGTCCGGGA ACACAAAGAC
    AATATTGGCT CCCAGTACCT GCTCAACTGG TGTGTGCAGA TCGCAAAGGG CATGAACTAC
    TTGGAGGACC GTCGCTTGGT GCACCGCGAC CTGGCAGCCA GGAACGTACT GGTGAAAACA
    CCGCAGCATG TCAAGATCAC AGATTTTGGG CTGGCCAAAC TGCTGGGTGC GGAAGAGAAA
    GAATACCATG CAGAAGGAGG CAAAGTGCCT ATCAAGTGGA TGGCATTGGA ATCAATTTTA
    CACAGAATCT ATACCCACCA GAGTGATGTC TGGAGCTACG GGGTGACTGT TTGGGAGTTG
    ATGACCTTTG GATCCAAGCC ATATGACGGA ATCCCTGCCA GCGAGATCTC CTCCATCCTG
    GAGAAAGGAG AACGCCTCCC TCAGCCACCC ATATGTACCA TCGATGTCTA CATGATCATG
    GTCAAGTGCT GGATGATAGA CGCAGATAGT CGCCCAAAGT TCCGTGAGTT GATCATCGAA
    TTCTCCAAAA TGGCCCGAGA CCCCCAGCGC TACCTTGTCA TTCAGGGGGA TGAAAGAATG
    CATTTGCCAA GTCCTACAGA CTCCAACTTC TACCGTGCCC TGATGGATGA AGAAGACATG
    GACGACGTGG TGGATGCCGA CGAGTACCTC ATCCCACAGC AGGGCTTCTT CAGCAGCCCC
    TCCACGTCAC GGACTCCCCT CCTGAGCTCT CTGAGTGCAA CCAGCAACAA TTCCACCGTG
    GCTTGCATTG ATAGAAATGG GCTGCAAAGC TGTCCCATCA AGGAAGACAG CTTCTTGCAG
    CGATACAGCT CAGACCCCAC AGGCGCCTTG ACTGAGGACA GCATAGACGA CACCTTCCTC
    CCAGTGCCTG AATACATAAA CCAGTCCGTT CCCAAAAGGC CCGCTGGCTC TGTGCAGAAT
    CCTGTCTATC ACAATCAGCC TCTGAACCCC GCGCCCAGCA GAGACCCACA CTACCAGGAC
    CCCCACAGCA CTGCAGTGGG CAACCCCGAG TATCTCAACA CTGTCCAGCC CACCTGTGTC
    AACAGCACAT TCGACAGCCC TGCCCACTGG GCCCAGAAAG GCAGCCACCA AATTAGCCTG
    GACAACCCTG ACTACCAGCA GGACTTCTTT CCCAAGGAAG CCAAGCCAAA TGGCATCTTT
    AAGGGCTCCA CAGCTGAAAA TGCAGAATAC CTAAGGGTCG CGCCACAAAG CAGTGAATTT
    ATTGGAGCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102753
    Clone ID Related Accession (Same CDS sequence) NM_001346900.2 , NM_001346900.1
    Accession Version NM_001346900.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-07
    Organism Homo sapiens(human)
    Product epidermal growth factor receptor isoform h
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC347326.1, AK294750.1, AC006977.3, AC073324.6, HQ912715.1, DA371223.1 and AW450657.1. On May 31, 2019 this sequence version replaced NM_001346900.1. Transcript Variant: This variant (8) uses a novel 5' terminal exon compared to variant 1. The encoded isoform (h) has a shorter and distinct N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK294750.1, SRR1803613.174821.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTTCAATA ACTGTGAGGT GGTCCTTGGG AATTTGGAAA TTACCTATGT GCAGAGGAAT 
    TATGATCTTT CCTTCTTAAA GACCATCCAG GAGGTGGCTG GTTATGTCCT CATTGCCCTC
    AACACAGTGG AGCGAATTCC TTTGGAAAAC CTGCAGATCA TCAGAGGAAA TATGTACTAC
    GAAAATTCCT ATGCCTTAGC AGTCTTATCT AACTATGATG CAAATAAAAC CGGACTGAAG
    GAGCTGCCCA TGAGAAATTT ACAGGAAATC CTGCATGGCG CCGTGCGGTT CAGCAACAAC
    CCTGCCCTGT GCAACGTGGA GAGCATCCAG TGGCGGGACA TAGTCAGCAG TGACTTTCTC
    AGCAACATGT CGATGGACTT CCAGAACCAC CTGGGCAGCT GCCAAAAGTG TGATCCAAGC
    TGTCCCAATG GGAGCTGCTG GGGTGCAGGA GAGGAGAACT GCCAGAAACT GACCAAAATC
    ATCTGTGCCC AGCAGTGCTC CGGGCGCTGC CGTGGCAAGT CCCCCAGTGA CTGCTGCCAC
    AACCAGTGTG CTGCAGGCTG CACAGGCCCC CGGGAGAGCG ACTGCCTGGT CTGCCGCAAA
    TTCCGAGACG AAGCCACGTG CAAGGACACC TGCCCCCCAC TCATGCTCTA CAACCCCACC
    ACGTACCAGA TGGATGTGAA CCCCGAGGGC AAATACAGCT TTGGTGCCAC CTGCGTGAAG
    AAGTGTCCCC GTAATTATGT GGTGACAGAT CACGGCTCGT GCGTCCGAGC CTGTGGGGCC
    GACAGCTATG AGATGGAGGA AGACGGCGTC CGCAAGTGTA AGAAGTGCGA AGGGCCTTGC
    CGCAAAGTGT GTAACGGAAT AGGTATTGGT GAATTTAAAG ACTCACTCTC CATAAATGCT
    ACGAATATTA AACACTTCAA AAACTGCACC TCCATCAGTG GCGATCTCCA CATCCTGCCG
    GTGGCATTTA GGGGTGACTC CTTCACACAT ACTCCTCCTC TGGATCCACA GGAACTGGAT
    ATTCTGAAAA CCGTAAAGGA AATCACAGGG TTTTTGCTGA TTCAGGCTTG GCCTGAAAAC
    AGGACGGACC TCCATGCCTT TGAGAACCTA GAAATCATAC GCGGCAGGAC CAAGCAACAT
    GGTCAGTTTT CTCTTGCAGT CGTCAGCCTG AACATAACAT CCTTGGGATT ACGCTCCCTC
    AAGGAGATAA GTGATGGAGA TGTGATAATT TCAGGAAACA AAAATTTGTG CTATGCAAAT
    ACAATAAACT GGAAAAAACT GTTTGGGACC TCCGGTCAGA AAACCAAAAT TATAAGCAAC
    AGAGGTGAAA ACAGCTGCAA GGCCACAGGC CAGGTCTGCC ATGCCTTGTG CTCCCCCGAG
    GGCTGCTGGG GCCCGGAGCC CAGGGACTGC GTCTCTTGCC GGAATGTCAG CCGAGGCAGG
    GAATGCGTGG ACAAGTGCAA CCTTCTGGAG GGTGAGCCAA GGGAGTTTGT GGAGAACTCT
    GAGTGCATAC AGTGCCACCC AGAGTGCCTG CCTCAGGCCA TGAACATCAC CTGCACAGGA
    CGGGGACCAG ACAACTGTAT CCAGTGTGCC CACTACATTG ACGGCCCCCA CTGCGTCAAG
    ACCTGCCCGG CAGGAGTCAT GGGAGAAAAC AACACCCTGG TCTGGAAGTA CGCAGACGCC
    GGCCATGTGT GCCACCTGTG CCATCCAAAC TGCACCTACG GATGCACTGG GCCAGGTCTT
    GAAGGCTGTC CAACGAATGG GCCTAAGATC CCGTCCATCG CCACTGGGAT GGTGGGGGCC
    CTCCTCTTGC TGCTGGTGGT GGCCCTGGGG ATCGGCCTCT TCATGCGAAG GCGCCACATC
    GTTCGGAAGC GCACGCTGCG GAGGCTGCTG CAGGAGAGGG AGCTTGTGGA GCCTCTTACA
    CCCAGTGGAG AAGCTCCCAA CCAAGCTCTC TTGAGGATCT TGAAGGAAAC TGAATTCAAA
    AAGATCAAAG TGCTGGGCTC CGGTGCGTTC GGCACGGTGT ATAAGGGACT CTGGATCCCA
    GAAGGTGAGA AAGTTAAAAT TCCCGTCGCT ATCAAGGAAT TAAGAGAAGC AACATCTCCG
    AAAGCCAACA AGGAAATCCT CGATGAAGCC TACGTGATGG CCAGCGTGGA CAACCCCCAC
    GTGTGCCGCC TGCTGGGCAT CTGCCTCACC TCCACCGTGC AGCTCATCAC GCAGCTCATG
    CCCTTCGGCT GCCTCCTGGA CTATGTCCGG GAACACAAAG ACAATATTGG CTCCCAGTAC
    CTGCTCAACT GGTGTGTGCA GATCGCAAAG GGCATGAACT ACTTGGAGGA CCGTCGCTTG
    GTGCACCGCG ACCTGGCAGC CAGGAACGTA CTGGTGAAAA CACCGCAGCA TGTCAAGATC
    ACAGATTTTG GGCTGGCCAA ACTGCTGGGT GCGGAAGAGA AAGAATACCA TGCAGAAGGA
    GGCAAAGTGC CTATCAAGTG GATGGCATTG GAATCAATTT TACACAGAAT CTATACCCAC
    CAGAGTGATG TCTGGAGCTA CGGGGTGACT GTTTGGGAGT TGATGACCTT TGGATCCAAG
    CCATATGACG GAATCCCTGC CAGCGAGATC TCCTCCATCC TGGAGAAAGG AGAACGCCTC
    CCTCAGCCAC CCATATGTAC CATCGATGTC TACATGATCA TGGTCAAGTG CTGGATGATA
    GACGCAGATA GTCGCCCAAA GTTCCGTGAG TTGATCATCG AATTCTCCAA AATGGCCCGA
    GACCCCCAGC GCTACCTTGT CATTCAGGGG GATGAAAGAA TGCATTTGCC AAGTCCTACA
    GACTCCAACT TCTACCGTGC CCTGATGGAT GAAGAAGACA TGGACGACGT GGTGGATGCC
    GACGAGTACC TCATCCCACA GCAGGGCTTC TTCAGCAGCC CCTCCACGTC ACGGACTCCC
    CTCCTGAGCT CTCTGAGTGC AACCAGCAAC AATTCCACCG TGGCTTGCAT TGATAGAAAT
    GGGCTGCAAA GCTGTCCCAT CAAGGAAGAC AGCTTCTTGC AGCGATACAG CTCAGACCCC
    ACAGGCGCCT TGACTGAGGA CAGCATAGAC GACACCTTCC TCCCAGTGCC TGAATACATA
    AACCAGTCCG TTCCCAAAAG GCCCGCTGGC TCTGTGCAGA ATCCTGTCTA TCACAATCAG
    CCTCTGAACC CCGCGCCCAG CAGAGACCCA CACTACCAGG ACCCCCACAG CACTGCAGTG
    GGCAACCCCG AGTATCTCAA CACTGTCCAG CCCACCTGTG TCAACAGCAC ATTCGACAGC
    CCTGCCCACT GGGCCCAGAA AGGCAGCCAC CAAATTAGCC TGGACAACCC TGACTACCAG
    CAGGACTTCT TTCCCAAGGA AGCCAAGCCA AATGGCATCT TTAAGGGCTC CACAGCTGAA
    AATGCAGAAT ACCTAAGGGT CGCGCCACAA AGCAGTGAAT TTATTGGAGC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333829.1
    CDS192..3665
    Translation

    Target ORF information:

    RefSeq Version NM_001346900.2
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346900.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTTCAATA ACTGTGAGGT GGTCCTTGGG AATTTGGAAA TTACCTATGT GCAGAGGAAT 
    TATGATCTTT CCTTCTTAAA GACCATCCAG GAGGTGGCTG GTTATGTCCT CATTGCCCTC
    AACACAGTGG AGCGAATTCC TTTGGAAAAC CTGCAGATCA TCAGAGGAAA TATGTACTAC
    GAAAATTCCT ATGCCTTAGC AGTCTTATCT AACTATGATG CAAATAAAAC CGGACTGAAG
    GAGCTGCCCA TGAGAAATTT ACAGGAAATC CTGCATGGCG CCGTGCGGTT CAGCAACAAC
    CCTGCCCTGT GCAACGTGGA GAGCATCCAG TGGCGGGACA TAGTCAGCAG TGACTTTCTC
    AGCAACATGT CGATGGACTT CCAGAACCAC CTGGGCAGCT GCCAAAAGTG TGATCCAAGC
    TGTCCCAATG GGAGCTGCTG GGGTGCAGGA GAGGAGAACT GCCAGAAACT GACCAAAATC
    ATCTGTGCCC AGCAGTGCTC CGGGCGCTGC CGTGGCAAGT CCCCCAGTGA CTGCTGCCAC
    AACCAGTGTG CTGCAGGCTG CACAGGCCCC CGGGAGAGCG ACTGCCTGGT CTGCCGCAAA
    TTCCGAGACG AAGCCACGTG CAAGGACACC TGCCCCCCAC TCATGCTCTA CAACCCCACC
    ACGTACCAGA TGGATGTGAA CCCCGAGGGC AAATACAGCT TTGGTGCCAC CTGCGTGAAG
    AAGTGTCCCC GTAATTATGT GGTGACAGAT CACGGCTCGT GCGTCCGAGC CTGTGGGGCC
    GACAGCTATG AGATGGAGGA AGACGGCGTC CGCAAGTGTA AGAAGTGCGA AGGGCCTTGC
    CGCAAAGTGT GTAACGGAAT AGGTATTGGT GAATTTAAAG ACTCACTCTC CATAAATGCT
    ACGAATATTA AACACTTCAA AAACTGCACC TCCATCAGTG GCGATCTCCA CATCCTGCCG
    GTGGCATTTA GGGGTGACTC CTTCACACAT ACTCCTCCTC TGGATCCACA GGAACTGGAT
    ATTCTGAAAA CCGTAAAGGA AATCACAGGG TTTTTGCTGA TTCAGGCTTG GCCTGAAAAC
    AGGACGGACC TCCATGCCTT TGAGAACCTA GAAATCATAC GCGGCAGGAC CAAGCAACAT
    GGTCAGTTTT CTCTTGCAGT CGTCAGCCTG AACATAACAT CCTTGGGATT ACGCTCCCTC
    AAGGAGATAA GTGATGGAGA TGTGATAATT TCAGGAAACA AAAATTTGTG CTATGCAAAT
    ACAATAAACT GGAAAAAACT GTTTGGGACC TCCGGTCAGA AAACCAAAAT TATAAGCAAC
    AGAGGTGAAA ACAGCTGCAA GGCCACAGGC CAGGTCTGCC ATGCCTTGTG CTCCCCCGAG
    GGCTGCTGGG GCCCGGAGCC CAGGGACTGC GTCTCTTGCC GGAATGTCAG CCGAGGCAGG
    GAATGCGTGG ACAAGTGCAA CCTTCTGGAG GGTGAGCCAA GGGAGTTTGT GGAGAACTCT
    GAGTGCATAC AGTGCCACCC AGAGTGCCTG CCTCAGGCCA TGAACATCAC CTGCACAGGA
    CGGGGACCAG ACAACTGTAT CCAGTGTGCC CACTACATTG ACGGCCCCCA CTGCGTCAAG
    ACCTGCCCGG CAGGAGTCAT GGGAGAAAAC AACACCCTGG TCTGGAAGTA CGCAGACGCC
    GGCCATGTGT GCCACCTGTG CCATCCAAAC TGCACCTACG GATGCACTGG GCCAGGTCTT
    GAAGGCTGTC CAACGAATGG GCCTAAGATC CCGTCCATCG CCACTGGGAT GGTGGGGGCC
    CTCCTCTTGC TGCTGGTGGT GGCCCTGGGG ATCGGCCTCT TCATGCGAAG GCGCCACATC
    GTTCGGAAGC GCACGCTGCG GAGGCTGCTG CAGGAGAGGG AGCTTGTGGA GCCTCTTACA
    CCCAGTGGAG AAGCTCCCAA CCAAGCTCTC TTGAGGATCT TGAAGGAAAC TGAATTCAAA
    AAGATCAAAG TGCTGGGCTC CGGTGCGTTC GGCACGGTGT ATAAGGGACT CTGGATCCCA
    GAAGGTGAGA AAGTTAAAAT TCCCGTCGCT ATCAAGGAAT TAAGAGAAGC AACATCTCCG
    AAAGCCAACA AGGAAATCCT CGATGAAGCC TACGTGATGG CCAGCGTGGA CAACCCCCAC
    GTGTGCCGCC TGCTGGGCAT CTGCCTCACC TCCACCGTGC AGCTCATCAC GCAGCTCATG
    CCCTTCGGCT GCCTCCTGGA CTATGTCCGG GAACACAAAG ACAATATTGG CTCCCAGTAC
    CTGCTCAACT GGTGTGTGCA GATCGCAAAG GGCATGAACT ACTTGGAGGA CCGTCGCTTG
    GTGCACCGCG ACCTGGCAGC CAGGAACGTA CTGGTGAAAA CACCGCAGCA TGTCAAGATC
    ACAGATTTTG GGCTGGCCAA ACTGCTGGGT GCGGAAGAGA AAGAATACCA TGCAGAAGGA
    GGCAAAGTGC CTATCAAGTG GATGGCATTG GAATCAATTT TACACAGAAT CTATACCCAC
    CAGAGTGATG TCTGGAGCTA CGGGGTGACT GTTTGGGAGT TGATGACCTT TGGATCCAAG
    CCATATGACG GAATCCCTGC CAGCGAGATC TCCTCCATCC TGGAGAAAGG AGAACGCCTC
    CCTCAGCCAC CCATATGTAC CATCGATGTC TACATGATCA TGGTCAAGTG CTGGATGATA
    GACGCAGATA GTCGCCCAAA GTTCCGTGAG TTGATCATCG AATTCTCCAA AATGGCCCGA
    GACCCCCAGC GCTACCTTGT CATTCAGGGG GATGAAAGAA TGCATTTGCC AAGTCCTACA
    GACTCCAACT TCTACCGTGC CCTGATGGAT GAAGAAGACA TGGACGACGT GGTGGATGCC
    GACGAGTACC TCATCCCACA GCAGGGCTTC TTCAGCAGCC CCTCCACGTC ACGGACTCCC
    CTCCTGAGCT CTCTGAGTGC AACCAGCAAC AATTCCACCG TGGCTTGCAT TGATAGAAAT
    GGGCTGCAAA GCTGTCCCAT CAAGGAAGAC AGCTTCTTGC AGCGATACAG CTCAGACCCC
    ACAGGCGCCT TGACTGAGGA CAGCATAGAC GACACCTTCC TCCCAGTGCC TGAATACATA
    AACCAGTCCG TTCCCAAAAG GCCCGCTGGC TCTGTGCAGA ATCCTGTCTA TCACAATCAG
    CCTCTGAACC CCGCGCCCAG CAGAGACCCA CACTACCAGG ACCCCCACAG CACTGCAGTG
    GGCAACCCCG AGTATCTCAA CACTGTCCAG CCCACCTGTG TCAACAGCAC ATTCGACAGC
    CCTGCCCACT GGGCCCAGAA AGGCAGCCAC CAAATTAGCC TGGACAACCC TGACTACCAG
    CAGGACTTCT TTCCCAAGGA AGCCAAGCCA AATGGCATCT TTAAGGGCTC CACAGCTGAA
    AATGCAGAAT ACCTAAGGGT CGCGCCACAA AGCAGTGAAT TTATTGGAGC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102753
    Clone ID Related Accession (Same CDS sequence) NM_001346900.2 , NM_001346900.1
    Accession Version NM_001346900.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product epidermal growth factor receptor isoform h
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC347326.1, AK294750.1, AC006977.3, AC073324.6, HQ912715.1, DA371223.1 and AW450657.1. Transcript Variant: This variant (8) uses a novel 5' terminal exon compared to variant 1. The encoded isoform (h) has a shorter and distinct N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK294750.1, SRR1803613.174821.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTTCAATA ACTGTGAGGT GGTCCTTGGG AATTTGGAAA TTACCTATGT GCAGAGGAAT 
    TATGATCTTT CCTTCTTAAA GACCATCCAG GAGGTGGCTG GTTATGTCCT CATTGCCCTC
    AACACAGTGG AGCGAATTCC TTTGGAAAAC CTGCAGATCA TCAGAGGAAA TATGTACTAC
    GAAAATTCCT ATGCCTTAGC AGTCTTATCT AACTATGATG CAAATAAAAC CGGACTGAAG
    GAGCTGCCCA TGAGAAATTT ACAGGAAATC CTGCATGGCG CCGTGCGGTT CAGCAACAAC
    CCTGCCCTGT GCAACGTGGA GAGCATCCAG TGGCGGGACA TAGTCAGCAG TGACTTTCTC
    AGCAACATGT CGATGGACTT CCAGAACCAC CTGGGCAGCT GCCAAAAGTG TGATCCAAGC
    TGTCCCAATG GGAGCTGCTG GGGTGCAGGA GAGGAGAACT GCCAGAAACT GACCAAAATC
    ATCTGTGCCC AGCAGTGCTC CGGGCGCTGC CGTGGCAAGT CCCCCAGTGA CTGCTGCCAC
    AACCAGTGTG CTGCAGGCTG CACAGGCCCC CGGGAGAGCG ACTGCCTGGT CTGCCGCAAA
    TTCCGAGACG AAGCCACGTG CAAGGACACC TGCCCCCCAC TCATGCTCTA CAACCCCACC
    ACGTACCAGA TGGATGTGAA CCCCGAGGGC AAATACAGCT TTGGTGCCAC CTGCGTGAAG
    AAGTGTCCCC GTAATTATGT GGTGACAGAT CACGGCTCGT GCGTCCGAGC CTGTGGGGCC
    GACAGCTATG AGATGGAGGA AGACGGCGTC CGCAAGTGTA AGAAGTGCGA AGGGCCTTGC
    CGCAAAGTGT GTAACGGAAT AGGTATTGGT GAATTTAAAG ACTCACTCTC CATAAATGCT
    ACGAATATTA AACACTTCAA AAACTGCACC TCCATCAGTG GCGATCTCCA CATCCTGCCG
    GTGGCATTTA GGGGTGACTC CTTCACACAT ACTCCTCCTC TGGATCCACA GGAACTGGAT
    ATTCTGAAAA CCGTAAAGGA AATCACAGGG TTTTTGCTGA TTCAGGCTTG GCCTGAAAAC
    AGGACGGACC TCCATGCCTT TGAGAACCTA GAAATCATAC GCGGCAGGAC CAAGCAACAT
    GGTCAGTTTT CTCTTGCAGT CGTCAGCCTG AACATAACAT CCTTGGGATT ACGCTCCCTC
    AAGGAGATAA GTGATGGAGA TGTGATAATT TCAGGAAACA AAAATTTGTG CTATGCAAAT
    ACAATAAACT GGAAAAAACT GTTTGGGACC TCCGGTCAGA AAACCAAAAT TATAAGCAAC
    AGAGGTGAAA ACAGCTGCAA GGCCACAGGC CAGGTCTGCC ATGCCTTGTG CTCCCCCGAG
    GGCTGCTGGG GCCCGGAGCC CAGGGACTGC GTCTCTTGCC GGAATGTCAG CCGAGGCAGG
    GAATGCGTGG ACAAGTGCAA CCTTCTGGAG GGTGAGCCAA GGGAGTTTGT GGAGAACTCT
    GAGTGCATAC AGTGCCACCC AGAGTGCCTG CCTCAGGCCA TGAACATCAC CTGCACAGGA
    CGGGGACCAG ACAACTGTAT CCAGTGTGCC CACTACATTG ACGGCCCCCA CTGCGTCAAG
    ACCTGCCCGG CAGGAGTCAT GGGAGAAAAC AACACCCTGG TCTGGAAGTA CGCAGACGCC
    GGCCATGTGT GCCACCTGTG CCATCCAAAC TGCACCTACG GATGCACTGG GCCAGGTCTT
    GAAGGCTGTC CAACGAATGG GCCTAAGATC CCGTCCATCG CCACTGGGAT GGTGGGGGCC
    CTCCTCTTGC TGCTGGTGGT GGCCCTGGGG ATCGGCCTCT TCATGCGAAG GCGCCACATC
    GTTCGGAAGC GCACGCTGCG GAGGCTGCTG CAGGAGAGGG AGCTTGTGGA GCCTCTTACA
    CCCAGTGGAG AAGCTCCCAA CCAAGCTCTC TTGAGGATCT TGAAGGAAAC TGAATTCAAA
    AAGATCAAAG TGCTGGGCTC CGGTGCGTTC GGCACGGTGT ATAAGGGACT CTGGATCCCA
    GAAGGTGAGA AAGTTAAAAT TCCCGTCGCT ATCAAGGAAT TAAGAGAAGC AACATCTCCG
    AAAGCCAACA AGGAAATCCT CGATGAAGCC TACGTGATGG CCAGCGTGGA CAACCCCCAC
    GTGTGCCGCC TGCTGGGCAT CTGCCTCACC TCCACCGTGC AGCTCATCAC GCAGCTCATG
    CCCTTCGGCT GCCTCCTGGA CTATGTCCGG GAACACAAAG ACAATATTGG CTCCCAGTAC
    CTGCTCAACT GGTGTGTGCA GATCGCAAAG GGCATGAACT ACTTGGAGGA CCGTCGCTTG
    GTGCACCGCG ACCTGGCAGC CAGGAACGTA CTGGTGAAAA CACCGCAGCA TGTCAAGATC
    ACAGATTTTG GGCTGGCCAA ACTGCTGGGT GCGGAAGAGA AAGAATACCA TGCAGAAGGA
    GGCAAAGTGC CTATCAAGTG GATGGCATTG GAATCAATTT TACACAGAAT CTATACCCAC
    CAGAGTGATG TCTGGAGCTA CGGGGTGACT GTTTGGGAGT TGATGACCTT TGGATCCAAG
    CCATATGACG GAATCCCTGC CAGCGAGATC TCCTCCATCC TGGAGAAAGG AGAACGCCTC
    CCTCAGCCAC CCATATGTAC CATCGATGTC TACATGATCA TGGTCAAGTG CTGGATGATA
    GACGCAGATA GTCGCCCAAA GTTCCGTGAG TTGATCATCG AATTCTCCAA AATGGCCCGA
    GACCCCCAGC GCTACCTTGT CATTCAGGGG GATGAAAGAA TGCATTTGCC AAGTCCTACA
    GACTCCAACT TCTACCGTGC CCTGATGGAT GAAGAAGACA TGGACGACGT GGTGGATGCC
    GACGAGTACC TCATCCCACA GCAGGGCTTC TTCAGCAGCC CCTCCACGTC ACGGACTCCC
    CTCCTGAGCT CTCTGAGTGC AACCAGCAAC AATTCCACCG TGGCTTGCAT TGATAGAAAT
    GGGCTGCAAA GCTGTCCCAT CAAGGAAGAC AGCTTCTTGC AGCGATACAG CTCAGACCCC
    ACAGGCGCCT TGACTGAGGA CAGCATAGAC GACACCTTCC TCCCAGTGCC TGAATACATA
    AACCAGTCCG TTCCCAAAAG GCCCGCTGGC TCTGTGCAGA ATCCTGTCTA TCACAATCAG
    CCTCTGAACC CCGCGCCCAG CAGAGACCCA CACTACCAGG ACCCCCACAG CACTGCAGTG
    GGCAACCCCG AGTATCTCAA CACTGTCCAG CCCACCTGTG TCAACAGCAC ATTCGACAGC
    CCTGCCCACT GGGCCCAGAA AGGCAGCCAC CAAATTAGCC TGGACAACCC TGACTACCAG
    CAGGACTTCT TTCCCAAGGA AGCCAAGCCA AATGGCATCT TTAAGGGCTC CACAGCTGAA
    AATGCAGAAT ACCTAAGGGT CGCGCCACAA AGCAGTGAAT TTATTGGAGC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333829.1
    CDS214..3687
    Translation

    Target ORF information:

    RefSeq Version NM_001346900.1
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346900.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTTCAATA ACTGTGAGGT GGTCCTTGGG AATTTGGAAA TTACCTATGT GCAGAGGAAT 
    TATGATCTTT CCTTCTTAAA GACCATCCAG GAGGTGGCTG GTTATGTCCT CATTGCCCTC
    AACACAGTGG AGCGAATTCC TTTGGAAAAC CTGCAGATCA TCAGAGGAAA TATGTACTAC
    GAAAATTCCT ATGCCTTAGC AGTCTTATCT AACTATGATG CAAATAAAAC CGGACTGAAG
    GAGCTGCCCA TGAGAAATTT ACAGGAAATC CTGCATGGCG CCGTGCGGTT CAGCAACAAC
    CCTGCCCTGT GCAACGTGGA GAGCATCCAG TGGCGGGACA TAGTCAGCAG TGACTTTCTC
    AGCAACATGT CGATGGACTT CCAGAACCAC CTGGGCAGCT GCCAAAAGTG TGATCCAAGC
    TGTCCCAATG GGAGCTGCTG GGGTGCAGGA GAGGAGAACT GCCAGAAACT GACCAAAATC
    ATCTGTGCCC AGCAGTGCTC CGGGCGCTGC CGTGGCAAGT CCCCCAGTGA CTGCTGCCAC
    AACCAGTGTG CTGCAGGCTG CACAGGCCCC CGGGAGAGCG ACTGCCTGGT CTGCCGCAAA
    TTCCGAGACG AAGCCACGTG CAAGGACACC TGCCCCCCAC TCATGCTCTA CAACCCCACC
    ACGTACCAGA TGGATGTGAA CCCCGAGGGC AAATACAGCT TTGGTGCCAC CTGCGTGAAG
    AAGTGTCCCC GTAATTATGT GGTGACAGAT CACGGCTCGT GCGTCCGAGC CTGTGGGGCC
    GACAGCTATG AGATGGAGGA AGACGGCGTC CGCAAGTGTA AGAAGTGCGA AGGGCCTTGC
    CGCAAAGTGT GTAACGGAAT AGGTATTGGT GAATTTAAAG ACTCACTCTC CATAAATGCT
    ACGAATATTA AACACTTCAA AAACTGCACC TCCATCAGTG GCGATCTCCA CATCCTGCCG
    GTGGCATTTA GGGGTGACTC CTTCACACAT ACTCCTCCTC TGGATCCACA GGAACTGGAT
    ATTCTGAAAA CCGTAAAGGA AATCACAGGG TTTTTGCTGA TTCAGGCTTG GCCTGAAAAC
    AGGACGGACC TCCATGCCTT TGAGAACCTA GAAATCATAC GCGGCAGGAC CAAGCAACAT
    GGTCAGTTTT CTCTTGCAGT CGTCAGCCTG AACATAACAT CCTTGGGATT ACGCTCCCTC
    AAGGAGATAA GTGATGGAGA TGTGATAATT TCAGGAAACA AAAATTTGTG CTATGCAAAT
    ACAATAAACT GGAAAAAACT GTTTGGGACC TCCGGTCAGA AAACCAAAAT TATAAGCAAC
    AGAGGTGAAA ACAGCTGCAA GGCCACAGGC CAGGTCTGCC ATGCCTTGTG CTCCCCCGAG
    GGCTGCTGGG GCCCGGAGCC CAGGGACTGC GTCTCTTGCC GGAATGTCAG CCGAGGCAGG
    GAATGCGTGG ACAAGTGCAA CCTTCTGGAG GGTGAGCCAA GGGAGTTTGT GGAGAACTCT
    GAGTGCATAC AGTGCCACCC AGAGTGCCTG CCTCAGGCCA TGAACATCAC CTGCACAGGA
    CGGGGACCAG ACAACTGTAT CCAGTGTGCC CACTACATTG ACGGCCCCCA CTGCGTCAAG
    ACCTGCCCGG CAGGAGTCAT GGGAGAAAAC AACACCCTGG TCTGGAAGTA CGCAGACGCC
    GGCCATGTGT GCCACCTGTG CCATCCAAAC TGCACCTACG GATGCACTGG GCCAGGTCTT
    GAAGGCTGTC CAACGAATGG GCCTAAGATC CCGTCCATCG CCACTGGGAT GGTGGGGGCC
    CTCCTCTTGC TGCTGGTGGT GGCCCTGGGG ATCGGCCTCT TCATGCGAAG GCGCCACATC
    GTTCGGAAGC GCACGCTGCG GAGGCTGCTG CAGGAGAGGG AGCTTGTGGA GCCTCTTACA
    CCCAGTGGAG AAGCTCCCAA CCAAGCTCTC TTGAGGATCT TGAAGGAAAC TGAATTCAAA
    AAGATCAAAG TGCTGGGCTC CGGTGCGTTC GGCACGGTGT ATAAGGGACT CTGGATCCCA
    GAAGGTGAGA AAGTTAAAAT TCCCGTCGCT ATCAAGGAAT TAAGAGAAGC AACATCTCCG
    AAAGCCAACA AGGAAATCCT CGATGAAGCC TACGTGATGG CCAGCGTGGA CAACCCCCAC
    GTGTGCCGCC TGCTGGGCAT CTGCCTCACC TCCACCGTGC AGCTCATCAC GCAGCTCATG
    CCCTTCGGCT GCCTCCTGGA CTATGTCCGG GAACACAAAG ACAATATTGG CTCCCAGTAC
    CTGCTCAACT GGTGTGTGCA GATCGCAAAG GGCATGAACT ACTTGGAGGA CCGTCGCTTG
    GTGCACCGCG ACCTGGCAGC CAGGAACGTA CTGGTGAAAA CACCGCAGCA TGTCAAGATC
    ACAGATTTTG GGCTGGCCAA ACTGCTGGGT GCGGAAGAGA AAGAATACCA TGCAGAAGGA
    GGCAAAGTGC CTATCAAGTG GATGGCATTG GAATCAATTT TACACAGAAT CTATACCCAC
    CAGAGTGATG TCTGGAGCTA CGGGGTGACT GTTTGGGAGT TGATGACCTT TGGATCCAAG
    CCATATGACG GAATCCCTGC CAGCGAGATC TCCTCCATCC TGGAGAAAGG AGAACGCCTC
    CCTCAGCCAC CCATATGTAC CATCGATGTC TACATGATCA TGGTCAAGTG CTGGATGATA
    GACGCAGATA GTCGCCCAAA GTTCCGTGAG TTGATCATCG AATTCTCCAA AATGGCCCGA
    GACCCCCAGC GCTACCTTGT CATTCAGGGG GATGAAAGAA TGCATTTGCC AAGTCCTACA
    GACTCCAACT TCTACCGTGC CCTGATGGAT GAAGAAGACA TGGACGACGT GGTGGATGCC
    GACGAGTACC TCATCCCACA GCAGGGCTTC TTCAGCAGCC CCTCCACGTC ACGGACTCCC
    CTCCTGAGCT CTCTGAGTGC AACCAGCAAC AATTCCACCG TGGCTTGCAT TGATAGAAAT
    GGGCTGCAAA GCTGTCCCAT CAAGGAAGAC AGCTTCTTGC AGCGATACAG CTCAGACCCC
    ACAGGCGCCT TGACTGAGGA CAGCATAGAC GACACCTTCC TCCCAGTGCC TGAATACATA
    AACCAGTCCG TTCCCAAAAG GCCCGCTGGC TCTGTGCAGA ATCCTGTCTA TCACAATCAG
    CCTCTGAACC CCGCGCCCAG CAGAGACCCA CACTACCAGG ACCCCCACAG CACTGCAGTG
    GGCAACCCCG AGTATCTCAA CACTGTCCAG CCCACCTGTG TCAACAGCAC ATTCGACAGC
    CCTGCCCACT GGGCCCAGAA AGGCAGCCAC CAAATTAGCC TGGACAACCC TGACTACCAG
    CAGGACTTCT TTCCCAAGGA AGCCAAGCCA AATGGCATCT TTAAGGGCTC CACAGCTGAA
    AATGCAGAAT ACCTAAGGGT CGCGCCACAA AGCAGTGAAT TTATTGGAGC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102757
    Clone ID Related Accession (Same CDS sequence) NM_005228.4 , NM_005228.5
    Accession Version NM_005228.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-31
    Organism Homo sapiens(human)
    Product epidermal growth factor receptor isoform a precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC094761.1, HQ912715.1, X00588.1, AF125253.1, AU137334.1, AC073324.6, DA371223.1 and AW450657.1. On Nov 9, 2016 this sequence version replaced NM_005228.3. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X00588.1, SRR1803616.241434.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
    AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
    CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
    GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
    GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
    ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
    AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
    CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
    GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
    GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
    GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
    AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
    TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
    ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
    GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
    GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
    GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
    TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
    GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
    AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
    CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
    GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
    CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
    GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
    CATCCAAACT GCACCTACGG ATGCACTGGG CCAGGTCTTG AAGGCTGTCC AACGAATGGG
    CCTAAGATCC CGTCCATCGC CACTGGGATG GTGGGGGCCC TCCTCTTGCT GCTGGTGGTG
    GCCCTGGGGA TCGGCCTCTT CATGCGAAGG CGCCACATCG TTCGGAAGCG CACGCTGCGG
    AGGCTGCTGC AGGAGAGGGA GCTTGTGGAG CCTCTTACAC CCAGTGGAGA AGCTCCCAAC
    CAAGCTCTCT TGAGGATCTT GAAGGAAACT GAATTCAAAA AGATCAAAGT GCTGGGCTCC
    GGTGCGTTCG GCACGGTGTA TAAGGGACTC TGGATCCCAG AAGGTGAGAA AGTTAAAATT
    CCCGTCGCTA TCAAGGAATT AAGAGAAGCA ACATCTCCGA AAGCCAACAA GGAAATCCTC
    GATGAAGCCT ACGTGATGGC CAGCGTGGAC AACCCCCACG TGTGCCGCCT GCTGGGCATC
    TGCCTCACCT CCACCGTGCA GCTCATCACG CAGCTCATGC CCTTCGGCTG CCTCCTGGAC
    TATGTCCGGG AACACAAAGA CAATATTGGC TCCCAGTACC TGCTCAACTG GTGTGTGCAG
    ATCGCAAAGG GCATGAACTA CTTGGAGGAC CGTCGCTTGG TGCACCGCGA CCTGGCAGCC
    AGGAACGTAC TGGTGAAAAC ACCGCAGCAT GTCAAGATCA CAGATTTTGG GCTGGCCAAA
    CTGCTGGGTG CGGAAGAGAA AGAATACCAT GCAGAAGGAG GCAAAGTGCC TATCAAGTGG
    ATGGCATTGG AATCAATTTT ACACAGAATC TATACCCACC AGAGTGATGT CTGGAGCTAC
    GGGGTGACTG TTTGGGAGTT GATGACCTTT GGATCCAAGC CATATGACGG AATCCCTGCC
    AGCGAGATCT CCTCCATCCT GGAGAAAGGA GAACGCCTCC CTCAGCCACC CATATGTACC
    ATCGATGTCT ACATGATCAT GGTCAAGTGC TGGATGATAG ACGCAGATAG TCGCCCAAAG
    TTCCGTGAGT TGATCATCGA ATTCTCCAAA ATGGCCCGAG ACCCCCAGCG CTACCTTGTC
    ATTCAGGGGG ATGAAAGAAT GCATTTGCCA AGTCCTACAG ACTCCAACTT CTACCGTGCC
    CTGATGGATG AAGAAGACAT GGACGACGTG GTGGATGCCG ACGAGTACCT CATCCCACAG
    CAGGGCTTCT TCAGCAGCCC CTCCACGTCA CGGACTCCCC TCCTGAGCTC TCTGAGTGCA
    ACCAGCAACA ATTCCACCGT GGCTTGCATT GATAGAAATG GGCTGCAAAG CTGTCCCATC
    AAGGAAGACA GCTTCTTGCA GCGATACAGC TCAGACCCCA CAGGCGCCTT GACTGAGGAC
    AGCATAGACG ACACCTTCCT CCCAGTGCCT GAATACATAA ACCAGTCCGT TCCCAAAAGG
    CCCGCTGGCT CTGTGCAGAA TCCTGTCTAT CACAATCAGC CTCTGAACCC CGCGCCCAGC
    AGAGACCCAC ACTACCAGGA CCCCCACAGC ACTGCAGTGG GCAACCCCGA GTATCTCAAC
    ACTGTCCAGC CCACCTGTGT CAACAGCACA TTCGACAGCC CTGCCCACTG GGCCCAGAAA
    GGCAGCCACC AAATTAGCCT GGACAACCCT GACTACCAGC AGGACTTCTT TCCCAAGGAA
    GCCAAGCCAA ATGGCATCTT TAAGGGCTCC ACAGCTGAAA ATGCAGAATA CCTAAGGGTC
    GCGCCACAAA GCAGTGAATT TATTGGAGCA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005219.2
    CDS258..3890
    Translation

    Target ORF information:

    RefSeq Version NM_005228.4
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005228.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
    AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
    CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
    GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
    GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
    ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
    AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
    CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
    GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
    GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
    GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
    AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
    TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
    ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
    GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
    GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
    GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
    TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
    GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
    AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
    CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
    GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
    CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
    GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
    CATCCAAACT GCACCTACGG ATGCACTGGG CCAGGTCTTG AAGGCTGTCC AACGAATGGG
    CCTAAGATCC CGTCCATCGC CACTGGGATG GTGGGGGCCC TCCTCTTGCT GCTGGTGGTG
    GCCCTGGGGA TCGGCCTCTT CATGCGAAGG CGCCACATCG TTCGGAAGCG CACGCTGCGG
    AGGCTGCTGC AGGAGAGGGA GCTTGTGGAG CCTCTTACAC CCAGTGGAGA AGCTCCCAAC
    CAAGCTCTCT TGAGGATCTT GAAGGAAACT GAATTCAAAA AGATCAAAGT GCTGGGCTCC
    GGTGCGTTCG GCACGGTGTA TAAGGGACTC TGGATCCCAG AAGGTGAGAA AGTTAAAATT
    CCCGTCGCTA TCAAGGAATT AAGAGAAGCA ACATCTCCGA AAGCCAACAA GGAAATCCTC
    GATGAAGCCT ACGTGATGGC CAGCGTGGAC AACCCCCACG TGTGCCGCCT GCTGGGCATC
    TGCCTCACCT CCACCGTGCA GCTCATCACG CAGCTCATGC CCTTCGGCTG CCTCCTGGAC
    TATGTCCGGG AACACAAAGA CAATATTGGC TCCCAGTACC TGCTCAACTG GTGTGTGCAG
    ATCGCAAAGG GCATGAACTA CTTGGAGGAC CGTCGCTTGG TGCACCGCGA CCTGGCAGCC
    AGGAACGTAC TGGTGAAAAC ACCGCAGCAT GTCAAGATCA CAGATTTTGG GCTGGCCAAA
    CTGCTGGGTG CGGAAGAGAA AGAATACCAT GCAGAAGGAG GCAAAGTGCC TATCAAGTGG
    ATGGCATTGG AATCAATTTT ACACAGAATC TATACCCACC AGAGTGATGT CTGGAGCTAC
    GGGGTGACTG TTTGGGAGTT GATGACCTTT GGATCCAAGC CATATGACGG AATCCCTGCC
    AGCGAGATCT CCTCCATCCT GGAGAAAGGA GAACGCCTCC CTCAGCCACC CATATGTACC
    ATCGATGTCT ACATGATCAT GGTCAAGTGC TGGATGATAG ACGCAGATAG TCGCCCAAAG
    TTCCGTGAGT TGATCATCGA ATTCTCCAAA ATGGCCCGAG ACCCCCAGCG CTACCTTGTC
    ATTCAGGGGG ATGAAAGAAT GCATTTGCCA AGTCCTACAG ACTCCAACTT CTACCGTGCC
    CTGATGGATG AAGAAGACAT GGACGACGTG GTGGATGCCG ACGAGTACCT CATCCCACAG
    CAGGGCTTCT TCAGCAGCCC CTCCACGTCA CGGACTCCCC TCCTGAGCTC TCTGAGTGCA
    ACCAGCAACA ATTCCACCGT GGCTTGCATT GATAGAAATG GGCTGCAAAG CTGTCCCATC
    AAGGAAGACA GCTTCTTGCA GCGATACAGC TCAGACCCCA CAGGCGCCTT GACTGAGGAC
    AGCATAGACG ACACCTTCCT CCCAGTGCCT GAATACATAA ACCAGTCCGT TCCCAAAAGG
    CCCGCTGGCT CTGTGCAGAA TCCTGTCTAT CACAATCAGC CTCTGAACCC CGCGCCCAGC
    AGAGACCCAC ACTACCAGGA CCCCCACAGC ACTGCAGTGG GCAACCCCGA GTATCTCAAC
    ACTGTCCAGC CCACCTGTGT CAACAGCACA TTCGACAGCC CTGCCCACTG GGCCCAGAAA
    GGCAGCCACC AAATTAGCCT GGACAACCCT GACTACCAGC AGGACTTCTT TCCCAAGGAA
    GCCAAGCCAA ATGGCATCTT TAAGGGCTCC ACAGCTGAAA ATGCAGAATA CCTAAGGGTC
    GCGCCACAAA GCAGTGAATT TATTGGAGCA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102757
    Clone ID Related Accession (Same CDS sequence) NM_005228.4 , NM_005228.5
    Accession Version NM_005228.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product epidermal growth factor receptor isoform a precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006977.3, BC094761.1, HQ912715.1, X00588.1, AF125253.1, AU137334.1, AC073324.6, DA371223.1 and AW450657.1. On Nov 22, 2018 this sequence version replaced NM_005228.4. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X00588.1, SRR1803616.241434.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000275493.7/ ENSP00000275493.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
    AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
    CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
    GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
    GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
    ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
    AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
    CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
    GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
    GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
    GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
    AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
    TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
    ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
    GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
    GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
    GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
    TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
    GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
    AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
    CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
    GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
    CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
    GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
    CATCCAAACT GCACCTACGG ATGCACTGGG CCAGGTCTTG AAGGCTGTCC AACGAATGGG
    CCTAAGATCC CGTCCATCGC CACTGGGATG GTGGGGGCCC TCCTCTTGCT GCTGGTGGTG
    GCCCTGGGGA TCGGCCTCTT CATGCGAAGG CGCCACATCG TTCGGAAGCG CACGCTGCGG
    AGGCTGCTGC AGGAGAGGGA GCTTGTGGAG CCTCTTACAC CCAGTGGAGA AGCTCCCAAC
    CAAGCTCTCT TGAGGATCTT GAAGGAAACT GAATTCAAAA AGATCAAAGT GCTGGGCTCC
    GGTGCGTTCG GCACGGTGTA TAAGGGACTC TGGATCCCAG AAGGTGAGAA AGTTAAAATT
    CCCGTCGCTA TCAAGGAATT AAGAGAAGCA ACATCTCCGA AAGCCAACAA GGAAATCCTC
    GATGAAGCCT ACGTGATGGC CAGCGTGGAC AACCCCCACG TGTGCCGCCT GCTGGGCATC
    TGCCTCACCT CCACCGTGCA GCTCATCACG CAGCTCATGC CCTTCGGCTG CCTCCTGGAC
    TATGTCCGGG AACACAAAGA CAATATTGGC TCCCAGTACC TGCTCAACTG GTGTGTGCAG
    ATCGCAAAGG GCATGAACTA CTTGGAGGAC CGTCGCTTGG TGCACCGCGA CCTGGCAGCC
    AGGAACGTAC TGGTGAAAAC ACCGCAGCAT GTCAAGATCA CAGATTTTGG GCTGGCCAAA
    CTGCTGGGTG CGGAAGAGAA AGAATACCAT GCAGAAGGAG GCAAAGTGCC TATCAAGTGG
    ATGGCATTGG AATCAATTTT ACACAGAATC TATACCCACC AGAGTGATGT CTGGAGCTAC
    GGGGTGACTG TTTGGGAGTT GATGACCTTT GGATCCAAGC CATATGACGG AATCCCTGCC
    AGCGAGATCT CCTCCATCCT GGAGAAAGGA GAACGCCTCC CTCAGCCACC CATATGTACC
    ATCGATGTCT ACATGATCAT GGTCAAGTGC TGGATGATAG ACGCAGATAG TCGCCCAAAG
    TTCCGTGAGT TGATCATCGA ATTCTCCAAA ATGGCCCGAG ACCCCCAGCG CTACCTTGTC
    ATTCAGGGGG ATGAAAGAAT GCATTTGCCA AGTCCTACAG ACTCCAACTT CTACCGTGCC
    CTGATGGATG AAGAAGACAT GGACGACGTG GTGGATGCCG ACGAGTACCT CATCCCACAG
    CAGGGCTTCT TCAGCAGCCC CTCCACGTCA CGGACTCCCC TCCTGAGCTC TCTGAGTGCA
    ACCAGCAACA ATTCCACCGT GGCTTGCATT GATAGAAATG GGCTGCAAAG CTGTCCCATC
    AAGGAAGACA GCTTCTTGCA GCGATACAGC TCAGACCCCA CAGGCGCCTT GACTGAGGAC
    AGCATAGACG ACACCTTCCT CCCAGTGCCT GAATACATAA ACCAGTCCGT TCCCAAAAGG
    CCCGCTGGCT CTGTGCAGAA TCCTGTCTAT CACAATCAGC CTCTGAACCC CGCGCCCAGC
    AGAGACCCAC ACTACCAGGA CCCCCACAGC ACTGCAGTGG GCAACCCCGA GTATCTCAAC
    ACTGTCCAGC CCACCTGTGT CAACAGCACA TTCGACAGCC CTGCCCACTG GGCCCAGAAA
    GGCAGCCACC AAATTAGCCT GGACAACCCT GACTACCAGC AGGACTTCTT TCCCAAGGAA
    GCCAAGCCAA ATGGCATCTT TAAGGGCTCC ACAGCTGAAA ATGCAGAATA CCTAAGGGTC
    GCGCCACAAA GCAGTGAATT TATTGGAGCA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005219.2
    CDS262..3894
    Translation

    Target ORF information:

    RefSeq Version NM_005228.5
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005228.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
    AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
    CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
    GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
    GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
    ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
    AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
    CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
    GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
    GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
    GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
    AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
    TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
    ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
    GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
    GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
    GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
    TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
    GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
    AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
    CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
    GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
    CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
    GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
    CATCCAAACT GCACCTACGG ATGCACTGGG CCAGGTCTTG AAGGCTGTCC AACGAATGGG
    CCTAAGATCC CGTCCATCGC CACTGGGATG GTGGGGGCCC TCCTCTTGCT GCTGGTGGTG
    GCCCTGGGGA TCGGCCTCTT CATGCGAAGG CGCCACATCG TTCGGAAGCG CACGCTGCGG
    AGGCTGCTGC AGGAGAGGGA GCTTGTGGAG CCTCTTACAC CCAGTGGAGA AGCTCCCAAC
    CAAGCTCTCT TGAGGATCTT GAAGGAAACT GAATTCAAAA AGATCAAAGT GCTGGGCTCC
    GGTGCGTTCG GCACGGTGTA TAAGGGACTC TGGATCCCAG AAGGTGAGAA AGTTAAAATT
    CCCGTCGCTA TCAAGGAATT AAGAGAAGCA ACATCTCCGA AAGCCAACAA GGAAATCCTC
    GATGAAGCCT ACGTGATGGC CAGCGTGGAC AACCCCCACG TGTGCCGCCT GCTGGGCATC
    TGCCTCACCT CCACCGTGCA GCTCATCACG CAGCTCATGC CCTTCGGCTG CCTCCTGGAC
    TATGTCCGGG AACACAAAGA CAATATTGGC TCCCAGTACC TGCTCAACTG GTGTGTGCAG
    ATCGCAAAGG GCATGAACTA CTTGGAGGAC CGTCGCTTGG TGCACCGCGA CCTGGCAGCC
    AGGAACGTAC TGGTGAAAAC ACCGCAGCAT GTCAAGATCA CAGATTTTGG GCTGGCCAAA
    CTGCTGGGTG CGGAAGAGAA AGAATACCAT GCAGAAGGAG GCAAAGTGCC TATCAAGTGG
    ATGGCATTGG AATCAATTTT ACACAGAATC TATACCCACC AGAGTGATGT CTGGAGCTAC
    GGGGTGACTG TTTGGGAGTT GATGACCTTT GGATCCAAGC CATATGACGG AATCCCTGCC
    AGCGAGATCT CCTCCATCCT GGAGAAAGGA GAACGCCTCC CTCAGCCACC CATATGTACC
    ATCGATGTCT ACATGATCAT GGTCAAGTGC TGGATGATAG ACGCAGATAG TCGCCCAAAG
    TTCCGTGAGT TGATCATCGA ATTCTCCAAA ATGGCCCGAG ACCCCCAGCG CTACCTTGTC
    ATTCAGGGGG ATGAAAGAAT GCATTTGCCA AGTCCTACAG ACTCCAACTT CTACCGTGCC
    CTGATGGATG AAGAAGACAT GGACGACGTG GTGGATGCCG ACGAGTACCT CATCCCACAG
    CAGGGCTTCT TCAGCAGCCC CTCCACGTCA CGGACTCCCC TCCTGAGCTC TCTGAGTGCA
    ACCAGCAACA ATTCCACCGT GGCTTGCATT GATAGAAATG GGCTGCAAAG CTGTCCCATC
    AAGGAAGACA GCTTCTTGCA GCGATACAGC TCAGACCCCA CAGGCGCCTT GACTGAGGAC
    AGCATAGACG ACACCTTCCT CCCAGTGCCT GAATACATAA ACCAGTCCGT TCCCAAAAGG
    CCCGCTGGCT CTGTGCAGAA TCCTGTCTAT CACAATCAGC CTCTGAACCC CGCGCCCAGC
    AGAGACCCAC ACTACCAGGA CCCCCACAGC ACTGCAGTGG GCAACCCCGA GTATCTCAAC
    ACTGTCCAGC CCACCTGTGT CAACAGCACA TTCGACAGCC CTGCCCACTG GGCCCAGAAA
    GGCAGCCACC AAATTAGCCT GGACAACCCT GACTACCAGC AGGACTTCTT TCCCAAGGAA
    GCCAAGCCAA ATGGCATCTT TAAGGGCTCC ACAGCTGAAA ATGCAGAATA CCTAAGGGTC
    GCGCCACAAA GCAGTGAATT TATTGGAGCA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102730
    Clone ID Related Accession (Same CDS sequence) NM_001346899.1
    Accession Version NM_001346899.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-15
    Organism Homo sapiens(human)
    Product epidermal growth factor receptor isoform g precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC094761.1, HQ912715.1, AC073324.6, DA371223.1 and AW450657.1. Transcript Variant: This variant (7) lacks an in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (g) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HQ912715.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153307, SAMN03267770 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGGCCAAA AGTGTGATCC AAGCTGTCCC AATGGGAGCT GCTGGGGTGC AGGAGAGGAG
    AACTGCCAGA AACTGACCAA AATCATCTGT GCCCAGCAGT GCTCCGGGCG CTGCCGTGGC
    AAGTCCCCCA GTGACTGCTG CCACAACCAG TGTGCTGCAG GCTGCACAGG CCCCCGGGAG
    AGCGACTGCC TGGTCTGCCG CAAATTCCGA GACGAAGCCA CGTGCAAGGA CACCTGCCCC
    CCACTCATGC TCTACAACCC CACCACGTAC CAGATGGATG TGAACCCCGA GGGCAAATAC
    AGCTTTGGTG CCACCTGCGT GAAGAAGTGT CCCCGTAATT ATGTGGTGAC AGATCACGGC
    TCGTGCGTCC GAGCCTGTGG GGCCGACAGC TATGAGATGG AGGAAGACGG CGTCCGCAAG
    TGTAAGAAGT GCGAAGGGCC TTGCCGCAAA GTGTGTAACG GAATAGGTAT TGGTGAATTT
    AAAGACTCAC TCTCCATAAA TGCTACGAAT ATTAAACACT TCAAAAACTG CACCTCCATC
    AGTGGCGATC TCCACATCCT GCCGGTGGCA TTTAGGGGTG ACTCCTTCAC ACATACTCCT
    CCTCTGGATC CACAGGAACT GGATATTCTG AAAACCGTAA AGGAAATCAC AGGGTTTTTG
    CTGATTCAGG CTTGGCCTGA AAACAGGACG GACCTCCATG CCTTTGAGAA CCTAGAAATC
    ATACGCGGCA GGACCAAGCA ACATGGTCAG TTTTCTCTTG CAGTCGTCAG CCTGAACATA
    ACATCCTTGG GATTACGCTC CCTCAAGGAG ATAAGTGATG GAGATGTGAT AATTTCAGGA
    AACAAAAATT TGTGCTATGC AAATACAATA AACTGGAAAA AACTGTTTGG GACCTCCGGT
    CAGAAAACCA AAATTATAAG CAACAGAGGT GAAAACAGCT GCAAGGCCAC AGGCCAGGTC
    TGCCATGCCT TGTGCTCCCC CGAGGGCTGC TGGGGCCCGG AGCCCAGGGA CTGCGTCTCT
    TGCCGGAATG TCAGCCGAGG CAGGGAATGC GTGGACAAGT GCAACCTTCT GGAGGGTGAG
    CCAAGGGAGT TTGTGGAGAA CTCTGAGTGC ATACAGTGCC ACCCAGAGTG CCTGCCTCAG
    GCCATGAACA TCACCTGCAC AGGACGGGGA CCAGACAACT GTATCCAGTG TGCCCACTAC
    ATTGACGGCC CCCACTGCGT CAAGACCTGC CCGGCAGGAG TCATGGGAGA AAACAACACC
    CTGGTCTGGA AGTACGCAGA CGCCGGCCAT GTGTGCCACC TGTGCCATCC AAACTGCACC
    TACGGATGCA CTGGGCCAGG TCTTGAAGGC TGTCCAACGA ATGGGCCTAA GATCCCGTCC
    ATCGCCACTG GGATGGTGGG GGCCCTCCTC TTGCTGCTGG TGGTGGCCCT GGGGATCGGC
    CTCTTCATGC GAAGGCGCCA CATCGTTCGG AAGCGCACGC TGCGGAGGCT GCTGCAGGAG
    AGGGAGCTTG TGGAGCCTCT TACACCCAGT GGAGAAGCTC CCAACCAAGC TCTCTTGAGG
    ATCTTGAAGG AAACTGAATT CAAAAAGATC AAAGTGCTGG GCTCCGGTGC GTTCGGCACG
    GTGTATAAGG GACTCTGGAT CCCAGAAGGT GAGAAAGTTA AAATTCCCGT CGCTATCAAG
    GAATTAAGAG AAGCAACATC TCCGAAAGCC AACAAGGAAA TCCTCGATGA AGCCTACGTG
    ATGGCCAGCG TGGACAACCC CCACGTGTGC CGCCTGCTGG GCATCTGCCT CACCTCCACC
    GTGCAGCTCA TCACGCAGCT CATGCCCTTC GGCTGCCTCC TGGACTATGT CCGGGAACAC
    AAAGACAATA TTGGCTCCCA GTACCTGCTC AACTGGTGTG TGCAGATCGC AAAGGGCATG
    AACTACTTGG AGGACCGTCG CTTGGTGCAC CGCGACCTGG CAGCCAGGAA CGTACTGGTG
    AAAACACCGC AGCATGTCAA GATCACAGAT TTTGGGCTGG CCAAACTGCT GGGTGCGGAA
    GAGAAAGAAT ACCATGCAGA AGGAGGCAAA GTGCCTATCA AGTGGATGGC ATTGGAATCA
    ATTTTACACA GAATCTATAC CCACCAGAGT GATGTCTGGA GCTACGGGGT GACTGTTTGG
    GAGTTGATGA CCTTTGGATC CAAGCCATAT GACGGAATCC CTGCCAGCGA GATCTCCTCC
    ATCCTGGAGA AAGGAGAACG CCTCCCTCAG CCACCCATAT GTACCATCGA TGTCTACATG
    ATCATGGTCA AGTGCTGGAT GATAGACGCA GATAGTCGCC CAAAGTTCCG TGAGTTGATC
    ATCGAATTCT CCAAAATGGC CCGAGACCCC CAGCGCTACC TTGTCATTCA GGGGGATGAA
    AGAATGCATT TGCCAAGTCC TACAGACTCC AACTTCTACC GTGCCCTGAT GGATGAAGAA
    GACATGGACG ACGTGGTGGA TGCCGACGAG TACCTCATCC CACAGCAGGG CTTCTTCAGC
    AGCCCCTCCA CGTCACGGAC TCCCCTCCTG AGCTCTCTGA GTGCAACCAG CAACAATTCC
    ACCGTGGCTT GCATTGATAG AAATGGGCTG CAAAGCTGTC CCATCAAGGA AGACAGCTTC
    TTGCAGCGAT ACAGCTCAGA CCCCACAGGC GCCTTGACTG AGGACAGCAT AGACGACACC
    TTCCTCCCAG TGCCTGAATA CATAAACCAG TCCGTTCCCA AAAGGCCCGC TGGCTCTGTG
    CAGAATCCTG TCTATCACAA TCAGCCTCTG AACCCCGCGC CCAGCAGAGA CCCACACTAC
    CAGGACCCCC ACAGCACTGC AGTGGGCAAC CCCGAGTATC TCAACACTGT CCAGCCCACC
    TGTGTCAACA GCACATTCGA CAGCCCTGCC CACTGGGCCC AGAAAGGCAG CCACCAAATT
    AGCCTGGACA ACCCTGACTA CCAGCAGGAC TTCTTTCCCA AGGAAGCCAA GCCAAATGGC
    ATCTTTAAGG GCTCCACAGC TGAAAATGCA GAATACCTAA GGGTCGCGCC ACAAAGCAGT
    GAATTTATTG GAGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333828.1
    CDS258..3755
    Translation

    Target ORF information:

    RefSeq Version NM_001346899.1
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346899.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGGCCAAA AGTGTGATCC AAGCTGTCCC AATGGGAGCT GCTGGGGTGC AGGAGAGGAG
    AACTGCCAGA AACTGACCAA AATCATCTGT GCCCAGCAGT GCTCCGGGCG CTGCCGTGGC
    AAGTCCCCCA GTGACTGCTG CCACAACCAG TGTGCTGCAG GCTGCACAGG CCCCCGGGAG
    AGCGACTGCC TGGTCTGCCG CAAATTCCGA GACGAAGCCA CGTGCAAGGA CACCTGCCCC
    CCACTCATGC TCTACAACCC CACCACGTAC CAGATGGATG TGAACCCCGA GGGCAAATAC
    AGCTTTGGTG CCACCTGCGT GAAGAAGTGT CCCCGTAATT ATGTGGTGAC AGATCACGGC
    TCGTGCGTCC GAGCCTGTGG GGCCGACAGC TATGAGATGG AGGAAGACGG CGTCCGCAAG
    TGTAAGAAGT GCGAAGGGCC TTGCCGCAAA GTGTGTAACG GAATAGGTAT TGGTGAATTT
    AAAGACTCAC TCTCCATAAA TGCTACGAAT ATTAAACACT TCAAAAACTG CACCTCCATC
    AGTGGCGATC TCCACATCCT GCCGGTGGCA TTTAGGGGTG ACTCCTTCAC ACATACTCCT
    CCTCTGGATC CACAGGAACT GGATATTCTG AAAACCGTAA AGGAAATCAC AGGGTTTTTG
    CTGATTCAGG CTTGGCCTGA AAACAGGACG GACCTCCATG CCTTTGAGAA CCTAGAAATC
    ATACGCGGCA GGACCAAGCA ACATGGTCAG TTTTCTCTTG CAGTCGTCAG CCTGAACATA
    ACATCCTTGG GATTACGCTC CCTCAAGGAG ATAAGTGATG GAGATGTGAT AATTTCAGGA
    AACAAAAATT TGTGCTATGC AAATACAATA AACTGGAAAA AACTGTTTGG GACCTCCGGT
    CAGAAAACCA AAATTATAAG CAACAGAGGT GAAAACAGCT GCAAGGCCAC AGGCCAGGTC
    TGCCATGCCT TGTGCTCCCC CGAGGGCTGC TGGGGCCCGG AGCCCAGGGA CTGCGTCTCT
    TGCCGGAATG TCAGCCGAGG CAGGGAATGC GTGGACAAGT GCAACCTTCT GGAGGGTGAG
    CCAAGGGAGT TTGTGGAGAA CTCTGAGTGC ATACAGTGCC ACCCAGAGTG CCTGCCTCAG
    GCCATGAACA TCACCTGCAC AGGACGGGGA CCAGACAACT GTATCCAGTG TGCCCACTAC
    ATTGACGGCC CCCACTGCGT CAAGACCTGC CCGGCAGGAG TCATGGGAGA AAACAACACC
    CTGGTCTGGA AGTACGCAGA CGCCGGCCAT GTGTGCCACC TGTGCCATCC AAACTGCACC
    TACGGATGCA CTGGGCCAGG TCTTGAAGGC TGTCCAACGA ATGGGCCTAA GATCCCGTCC
    ATCGCCACTG GGATGGTGGG GGCCCTCCTC TTGCTGCTGG TGGTGGCCCT GGGGATCGGC
    CTCTTCATGC GAAGGCGCCA CATCGTTCGG AAGCGCACGC TGCGGAGGCT GCTGCAGGAG
    AGGGAGCTTG TGGAGCCTCT TACACCCAGT GGAGAAGCTC CCAACCAAGC TCTCTTGAGG
    ATCTTGAAGG AAACTGAATT CAAAAAGATC AAAGTGCTGG GCTCCGGTGC GTTCGGCACG
    GTGTATAAGG GACTCTGGAT CCCAGAAGGT GAGAAAGTTA AAATTCCCGT CGCTATCAAG
    GAATTAAGAG AAGCAACATC TCCGAAAGCC AACAAGGAAA TCCTCGATGA AGCCTACGTG
    ATGGCCAGCG TGGACAACCC CCACGTGTGC CGCCTGCTGG GCATCTGCCT CACCTCCACC
    GTGCAGCTCA TCACGCAGCT CATGCCCTTC GGCTGCCTCC TGGACTATGT CCGGGAACAC
    AAAGACAATA TTGGCTCCCA GTACCTGCTC AACTGGTGTG TGCAGATCGC AAAGGGCATG
    AACTACTTGG AGGACCGTCG CTTGGTGCAC CGCGACCTGG CAGCCAGGAA CGTACTGGTG
    AAAACACCGC AGCATGTCAA GATCACAGAT TTTGGGCTGG CCAAACTGCT GGGTGCGGAA
    GAGAAAGAAT ACCATGCAGA AGGAGGCAAA GTGCCTATCA AGTGGATGGC ATTGGAATCA
    ATTTTACACA GAATCTATAC CCACCAGAGT GATGTCTGGA GCTACGGGGT GACTGTTTGG
    GAGTTGATGA CCTTTGGATC CAAGCCATAT GACGGAATCC CTGCCAGCGA GATCTCCTCC
    ATCCTGGAGA AAGGAGAACG CCTCCCTCAG CCACCCATAT GTACCATCGA TGTCTACATG
    ATCATGGTCA AGTGCTGGAT GATAGACGCA GATAGTCGCC CAAAGTTCCG TGAGTTGATC
    ATCGAATTCT CCAAAATGGC CCGAGACCCC CAGCGCTACC TTGTCATTCA GGGGGATGAA
    AGAATGCATT TGCCAAGTCC TACAGACTCC AACTTCTACC GTGCCCTGAT GGATGAAGAA
    GACATGGACG ACGTGGTGGA TGCCGACGAG TACCTCATCC CACAGCAGGG CTTCTTCAGC
    AGCCCCTCCA CGTCACGGAC TCCCCTCCTG AGCTCTCTGA GTGCAACCAG CAACAATTCC
    ACCGTGGCTT GCATTGATAG AAATGGGCTG CAAAGCTGTC CCATCAAGGA AGACAGCTTC
    TTGCAGCGAT ACAGCTCAGA CCCCACAGGC GCCTTGACTG AGGACAGCAT AGACGACACC
    TTCCTCCCAG TGCCTGAATA CATAAACCAG TCCGTTCCCA AAAGGCCCGC TGGCTCTGTG
    CAGAATCCTG TCTATCACAA TCAGCCTCTG AACCCCGCGC CCAGCAGAGA CCCACACTAC
    CAGGACCCCC ACAGCACTGC AGTGGGCAAC CCCGAGTATC TCAACACTGT CCAGCCCACC
    TGTGTCAACA GCACATTCGA CAGCCCTGCC CACTGGGCCC AGAAAGGCAG CCACCAAATT
    AGCCTGGACA ACCCTGACTA CCAGCAGGAC TTCTTTCCCA AGGAAGCCAA GCCAAATGGC
    ATCTTTAAGG GCTCCACAGC TGAAAATGCA GAATACCTAA GGGTCGCGCC ACAAAGCAGT
    GAATTTATTG GAGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102762
    Clone ID Related Accession (Same CDS sequence) NM_001346898.2 , NM_001346898.1
    Accession Version NM_001346898.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3411bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-15
    Organism Homo sapiens(human)
    Product epidermal growth factor receptor isoform f precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006977.3, BC094761.1, HQ912715.1, GU255993.1 and AC073324.6. On May 31, 2019 this sequence version replaced NM_001346898.1. Transcript Variant: This variant (6) has a 3' terminal exon that extends past a splice site that is used in variant 1. The encoded isoform (f) has a shorter and distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GU255993.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
    AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
    CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
    GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
    GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
    ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
    AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
    CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
    GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
    GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
    GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
    AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
    TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
    ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
    GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
    GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
    GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
    TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
    GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
    AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
    CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
    GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
    CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
    GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
    CATCCAAACT GCACCTACGG ATGCACTGGG CCAGGTCTTG AAGGCTGTCC AACGAATGGG
    CCTAAGATCC CGTCCATCGC CACTGGGATG GTGGGGGCCC TCCTCTTGCT GCTGGTGGTG
    GCCCTGGGGA TCGGCCTCTT CATGCGAAGG CGCCACATCG TTCGGAAGCG CACGCTGCGG
    AGGCTGCTGC AGGAGAGGGA GCTTGTGGAG CCTCTTACAC CCAGTGGAGA AGCTCCCAAC
    CAAGCTCTCT TGAGGATCTT GAAGGAAACT GAATTCAAAA AGATCAAAGT GCTGGGCTCC
    GGTGCGTTCG GCACGGTGTA TAAGGGACTC TGGATCCCAG AAGGTGAGAA AGTTAAAATT
    CCCGTCGCTA TCAAGGAATT AAGAGAAGCA ACATCTCCGA AAGCCAACAA GGAAATCCTC
    GATGAAGCCT ACGTGATGGC CAGCGTGGAC AACCCCCACG TGTGCCGCCT GCTGGGCATC
    TGCCTCACCT CCACCGTGCA GCTCATCACG CAGCTCATGC CCTTCGGCTG CCTCCTGGAC
    TATGTCCGGG AACACAAAGA CAATATTGGC TCCCAGTACC TGCTCAACTG GTGTGTGCAG
    ATCGCAAAGG GCATGAACTA CTTGGAGGAC CGTCGCTTGG TGCACCGCGA CCTGGCAGCC
    AGGAACGTAC TGGTGAAAAC ACCGCAGCAT GTCAAGATCA CAGATTTTGG GCTGGCCAAA
    CTGCTGGGTG CGGAAGAGAA AGAATACCAT GCAGAAGGAG GCAAAGTGCC TATCAAGTGG
    ATGGCATTGG AATCAATTTT ACACAGAATC TATACCCACC AGAGTGATGT CTGGAGCTAC
    GGGGTGACTG TTTGGGAGTT GATGACCTTT GGATCCAAGC CATATGACGG AATCCCTGCC
    AGCGAGATCT CCTCCATCCT GGAGAAAGGA GAACGCCTCC CTCAGCCACC CATATGTACC
    ATCGATGTCT ACATGATCAT GGTCAAGTGC TGGATGATAG ACGCAGATAG TCGCCCAAAG
    TTCCGTGAGT TGATCATCGA ATTCTCCAAA ATGGCCCGAG ACCCCCAGCG CTACCTTGTC
    ATTCAGGGGG ATGAAAGAAT GCATTTGCCA AGTCCTACAG ACTCCAACTT CTACCGTGCC
    CTGATGGATG AAGAAGACAT GGACGACGTG GTGGATGCCG ACGAGTACCT CATCCCACAG
    CAGGGCTTCT TCAGCAGCCC CTCCACGTCA CGGACTCCCC TCCTGAGCTC TCTGAGTGCA
    ACCAGCAACA ATTCCACCGT GGCTTGCATT GATAGAAATG GGCTGCAAAG CTGTCCCATC
    AAGGAAGACA GCTTCTTGCA GCGATACAGC TCAGACCCCA CAGGCGCCTT GACTGAGGAC
    AGCATAGACG ACACCTTCCT CCCAGTGCCT GGTGAGTGGC TTGTCTGGAA ACAGTCCTGC
    TCCTCAACCT CCTCGACCCA CTCAGCAGCA GCCAGTCTCC AGTGTCCAAG CCAGGTGCTC
    CCTCCAGCAT CTCCAGAGGG GGAAACAGTG GCAGATTTGC AGACACAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333827.1
    CDS262..3672
    Translation

    Target ORF information:

    RefSeq Version NM_001346898.2
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346898.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
    AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
    CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
    GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
    GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
    ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
    AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
    CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
    GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
    GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
    GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
    AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
    TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
    ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
    GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
    GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
    GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
    TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
    GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
    AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
    CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
    GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
    CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
    GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
    CATCCAAACT GCACCTACGG ATGCACTGGG CCAGGTCTTG AAGGCTGTCC AACGAATGGG
    CCTAAGATCC CGTCCATCGC CACTGGGATG GTGGGGGCCC TCCTCTTGCT GCTGGTGGTG
    GCCCTGGGGA TCGGCCTCTT CATGCGAAGG CGCCACATCG TTCGGAAGCG CACGCTGCGG
    AGGCTGCTGC AGGAGAGGGA GCTTGTGGAG CCTCTTACAC CCAGTGGAGA AGCTCCCAAC
    CAAGCTCTCT TGAGGATCTT GAAGGAAACT GAATTCAAAA AGATCAAAGT GCTGGGCTCC
    GGTGCGTTCG GCACGGTGTA TAAGGGACTC TGGATCCCAG AAGGTGAGAA AGTTAAAATT
    CCCGTCGCTA TCAAGGAATT AAGAGAAGCA ACATCTCCGA AAGCCAACAA GGAAATCCTC
    GATGAAGCCT ACGTGATGGC CAGCGTGGAC AACCCCCACG TGTGCCGCCT GCTGGGCATC
    TGCCTCACCT CCACCGTGCA GCTCATCACG CAGCTCATGC CCTTCGGCTG CCTCCTGGAC
    TATGTCCGGG AACACAAAGA CAATATTGGC TCCCAGTACC TGCTCAACTG GTGTGTGCAG
    ATCGCAAAGG GCATGAACTA CTTGGAGGAC CGTCGCTTGG TGCACCGCGA CCTGGCAGCC
    AGGAACGTAC TGGTGAAAAC ACCGCAGCAT GTCAAGATCA CAGATTTTGG GCTGGCCAAA
    CTGCTGGGTG CGGAAGAGAA AGAATACCAT GCAGAAGGAG GCAAAGTGCC TATCAAGTGG
    ATGGCATTGG AATCAATTTT ACACAGAATC TATACCCACC AGAGTGATGT CTGGAGCTAC
    GGGGTGACTG TTTGGGAGTT GATGACCTTT GGATCCAAGC CATATGACGG AATCCCTGCC
    AGCGAGATCT CCTCCATCCT GGAGAAAGGA GAACGCCTCC CTCAGCCACC CATATGTACC
    ATCGATGTCT ACATGATCAT GGTCAAGTGC TGGATGATAG ACGCAGATAG TCGCCCAAAG
    TTCCGTGAGT TGATCATCGA ATTCTCCAAA ATGGCCCGAG ACCCCCAGCG CTACCTTGTC
    ATTCAGGGGG ATGAAAGAAT GCATTTGCCA AGTCCTACAG ACTCCAACTT CTACCGTGCC
    CTGATGGATG AAGAAGACAT GGACGACGTG GTGGATGCCG ACGAGTACCT CATCCCACAG
    CAGGGCTTCT TCAGCAGCCC CTCCACGTCA CGGACTCCCC TCCTGAGCTC TCTGAGTGCA
    ACCAGCAACA ATTCCACCGT GGCTTGCATT GATAGAAATG GGCTGCAAAG CTGTCCCATC
    AAGGAAGACA GCTTCTTGCA GCGATACAGC TCAGACCCCA CAGGCGCCTT GACTGAGGAC
    AGCATAGACG ACACCTTCCT CCCAGTGCCT GGTGAGTGGC TTGTCTGGAA ACAGTCCTGC
    TCCTCAACCT CCTCGACCCA CTCAGCAGCA GCCAGTCTCC AGTGTCCAAG CCAGGTGCTC
    CCTCCAGCAT CTCCAGAGGG GGAAACAGTG GCAGATTTGC AGACACAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102762
    Clone ID Related Accession (Same CDS sequence) NM_001346898.2 , NM_001346898.1
    Accession Version NM_001346898.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3411bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product epidermal growth factor receptor isoform f precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC094761.1, HQ912715.1, GU255993.1 and AC073324.6. Transcript Variant: This variant (6) has a 3' terminal exon that extends past a splice site that is used in variant 1. The encoded isoform (f) has a shorter and distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GU255993.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
    AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
    CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
    GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
    GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
    ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
    AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
    CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
    GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
    GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
    GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
    AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
    TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
    ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
    GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
    GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
    GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
    TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
    GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
    AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
    CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
    GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
    CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
    GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
    CATCCAAACT GCACCTACGG ATGCACTGGG CCAGGTCTTG AAGGCTGTCC AACGAATGGG
    CCTAAGATCC CGTCCATCGC CACTGGGATG GTGGGGGCCC TCCTCTTGCT GCTGGTGGTG
    GCCCTGGGGA TCGGCCTCTT CATGCGAAGG CGCCACATCG TTCGGAAGCG CACGCTGCGG
    AGGCTGCTGC AGGAGAGGGA GCTTGTGGAG CCTCTTACAC CCAGTGGAGA AGCTCCCAAC
    CAAGCTCTCT TGAGGATCTT GAAGGAAACT GAATTCAAAA AGATCAAAGT GCTGGGCTCC
    GGTGCGTTCG GCACGGTGTA TAAGGGACTC TGGATCCCAG AAGGTGAGAA AGTTAAAATT
    CCCGTCGCTA TCAAGGAATT AAGAGAAGCA ACATCTCCGA AAGCCAACAA GGAAATCCTC
    GATGAAGCCT ACGTGATGGC CAGCGTGGAC AACCCCCACG TGTGCCGCCT GCTGGGCATC
    TGCCTCACCT CCACCGTGCA GCTCATCACG CAGCTCATGC CCTTCGGCTG CCTCCTGGAC
    TATGTCCGGG AACACAAAGA CAATATTGGC TCCCAGTACC TGCTCAACTG GTGTGTGCAG
    ATCGCAAAGG GCATGAACTA CTTGGAGGAC CGTCGCTTGG TGCACCGCGA CCTGGCAGCC
    AGGAACGTAC TGGTGAAAAC ACCGCAGCAT GTCAAGATCA CAGATTTTGG GCTGGCCAAA
    CTGCTGGGTG CGGAAGAGAA AGAATACCAT GCAGAAGGAG GCAAAGTGCC TATCAAGTGG
    ATGGCATTGG AATCAATTTT ACACAGAATC TATACCCACC AGAGTGATGT CTGGAGCTAC
    GGGGTGACTG TTTGGGAGTT GATGACCTTT GGATCCAAGC CATATGACGG AATCCCTGCC
    AGCGAGATCT CCTCCATCCT GGAGAAAGGA GAACGCCTCC CTCAGCCACC CATATGTACC
    ATCGATGTCT ACATGATCAT GGTCAAGTGC TGGATGATAG ACGCAGATAG TCGCCCAAAG
    TTCCGTGAGT TGATCATCGA ATTCTCCAAA ATGGCCCGAG ACCCCCAGCG CTACCTTGTC
    ATTCAGGGGG ATGAAAGAAT GCATTTGCCA AGTCCTACAG ACTCCAACTT CTACCGTGCC
    CTGATGGATG AAGAAGACAT GGACGACGTG GTGGATGCCG ACGAGTACCT CATCCCACAG
    CAGGGCTTCT TCAGCAGCCC CTCCACGTCA CGGACTCCCC TCCTGAGCTC TCTGAGTGCA
    ACCAGCAACA ATTCCACCGT GGCTTGCATT GATAGAAATG GGCTGCAAAG CTGTCCCATC
    AAGGAAGACA GCTTCTTGCA GCGATACAGC TCAGACCCCA CAGGCGCCTT GACTGAGGAC
    AGCATAGACG ACACCTTCCT CCCAGTGCCT GGTGAGTGGC TTGTCTGGAA ACAGTCCTGC
    TCCTCAACCT CCTCGACCCA CTCAGCAGCA GCCAGTCTCC AGTGTCCAAG CCAGGTGCTC
    CCTCCAGCAT CTCCAGAGGG GGAAACAGTG GCAGATTTGC AGACACAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333827.1
    CDS258..3668
    Translation

    Target ORF information:

    RefSeq Version NM_001346898.1
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346898.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
    AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
    CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
    GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
    GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
    ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
    AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
    CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
    GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
    GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
    GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
    AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
    TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
    ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
    GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
    GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
    GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
    TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
    GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
    AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
    CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
    GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
    CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
    GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
    CATCCAAACT GCACCTACGG ATGCACTGGG CCAGGTCTTG AAGGCTGTCC AACGAATGGG
    CCTAAGATCC CGTCCATCGC CACTGGGATG GTGGGGGCCC TCCTCTTGCT GCTGGTGGTG
    GCCCTGGGGA TCGGCCTCTT CATGCGAAGG CGCCACATCG TTCGGAAGCG CACGCTGCGG
    AGGCTGCTGC AGGAGAGGGA GCTTGTGGAG CCTCTTACAC CCAGTGGAGA AGCTCCCAAC
    CAAGCTCTCT TGAGGATCTT GAAGGAAACT GAATTCAAAA AGATCAAAGT GCTGGGCTCC
    GGTGCGTTCG GCACGGTGTA TAAGGGACTC TGGATCCCAG AAGGTGAGAA AGTTAAAATT
    CCCGTCGCTA TCAAGGAATT AAGAGAAGCA ACATCTCCGA AAGCCAACAA GGAAATCCTC
    GATGAAGCCT ACGTGATGGC CAGCGTGGAC AACCCCCACG TGTGCCGCCT GCTGGGCATC
    TGCCTCACCT CCACCGTGCA GCTCATCACG CAGCTCATGC CCTTCGGCTG CCTCCTGGAC
    TATGTCCGGG AACACAAAGA CAATATTGGC TCCCAGTACC TGCTCAACTG GTGTGTGCAG
    ATCGCAAAGG GCATGAACTA CTTGGAGGAC CGTCGCTTGG TGCACCGCGA CCTGGCAGCC
    AGGAACGTAC TGGTGAAAAC ACCGCAGCAT GTCAAGATCA CAGATTTTGG GCTGGCCAAA
    CTGCTGGGTG CGGAAGAGAA AGAATACCAT GCAGAAGGAG GCAAAGTGCC TATCAAGTGG
    ATGGCATTGG AATCAATTTT ACACAGAATC TATACCCACC AGAGTGATGT CTGGAGCTAC
    GGGGTGACTG TTTGGGAGTT GATGACCTTT GGATCCAAGC CATATGACGG AATCCCTGCC
    AGCGAGATCT CCTCCATCCT GGAGAAAGGA GAACGCCTCC CTCAGCCACC CATATGTACC
    ATCGATGTCT ACATGATCAT GGTCAAGTGC TGGATGATAG ACGCAGATAG TCGCCCAAAG
    TTCCGTGAGT TGATCATCGA ATTCTCCAAA ATGGCCCGAG ACCCCCAGCG CTACCTTGTC
    ATTCAGGGGG ATGAAAGAAT GCATTTGCCA AGTCCTACAG ACTCCAACTT CTACCGTGCC
    CTGATGGATG AAGAAGACAT GGACGACGTG GTGGATGCCG ACGAGTACCT CATCCCACAG
    CAGGGCTTCT TCAGCAGCCC CTCCACGTCA CGGACTCCCC TCCTGAGCTC TCTGAGTGCA
    ACCAGCAACA ATTCCACCGT GGCTTGCATT GATAGAAATG GGCTGCAAAG CTGTCCCATC
    AAGGAAGACA GCTTCTTGCA GCGATACAGC TCAGACCCCA CAGGCGCCTT GACTGAGGAC
    AGCATAGACG ACACCTTCCT CCCAGTGCCT GGTGAGTGGC TTGTCTGGAA ACAGTCCTGC
    TCCTCAACCT CCTCGACCCA CTCAGCAGCA GCCAGTCTCC AGTGTCCAAG CCAGGTGCTC
    CCTCCAGCAT CTCCAGAGGG GGAAACAGTG GCAGATTTGC AGACACAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102780
    Clone ID Related Accession (Same CDS sequence) NM_001346897.1 , NM_001346897.2
    Accession Version NM_001346897.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product epidermal growth factor receptor isoform e precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC094761.1, AC006977.3 and AC073324.6. Transcript Variant: This variant (5) lacks an in-frame exon in the 5' coding region and its 3' terminal exon extends past a splice site that is used in variant 1. The encoded isoform (e) is shorter and has a distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC094761.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153307, SAMN03267770 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGGCCAAA AGTGTGATCC AAGCTGTCCC AATGGGAGCT GCTGGGGTGC AGGAGAGGAG
    AACTGCCAGA AACTGACCAA AATCATCTGT GCCCAGCAGT GCTCCGGGCG CTGCCGTGGC
    AAGTCCCCCA GTGACTGCTG CCACAACCAG TGTGCTGCAG GCTGCACAGG CCCCCGGGAG
    AGCGACTGCC TGGTCTGCCG CAAATTCCGA GACGAAGCCA CGTGCAAGGA CACCTGCCCC
    CCACTCATGC TCTACAACCC CACCACGTAC CAGATGGATG TGAACCCCGA GGGCAAATAC
    AGCTTTGGTG CCACCTGCGT GAAGAAGTGT CCCCGTAATT ATGTGGTGAC AGATCACGGC
    TCGTGCGTCC GAGCCTGTGG GGCCGACAGC TATGAGATGG AGGAAGACGG CGTCCGCAAG
    TGTAAGAAGT GCGAAGGGCC TTGCCGCAAA GTGTGTAACG GAATAGGTAT TGGTGAATTT
    AAAGACTCAC TCTCCATAAA TGCTACGAAT ATTAAACACT TCAAAAACTG CACCTCCATC
    AGTGGCGATC TCCACATCCT GCCGGTGGCA TTTAGGGGTG ACTCCTTCAC ACATACTCCT
    CCTCTGGATC CACAGGAACT GGATATTCTG AAAACCGTAA AGGAAATCAC AGGGTTTTTG
    CTGATTCAGG CTTGGCCTGA AAACAGGACG GACCTCCATG CCTTTGAGAA CCTAGAAATC
    ATACGCGGCA GGACCAAGCA ACATGGTCAG TTTTCTCTTG CAGTCGTCAG CCTGAACATA
    ACATCCTTGG GATTACGCTC CCTCAAGGAG ATAAGTGATG GAGATGTGAT AATTTCAGGA
    AACAAAAATT TGTGCTATGC AAATACAATA AACTGGAAAA AACTGTTTGG GACCTCCGGT
    CAGAAAACCA AAATTATAAG CAACAGAGGT GAAAACAGCT GCAAGGCCAC AGGCCAGGTC
    TGCCATGCCT TGTGCTCCCC CGAGGGCTGC TGGGGCCCGG AGCCCAGGGA CTGCGTCTCT
    TGCCGGAATG TCAGCCGAGG CAGGGAATGC GTGGACAAGT GCAACCTTCT GGAGGGTGAG
    CCAAGGGAGT TTGTGGAGAA CTCTGAGTGC ATACAGTGCC ACCCAGAGTG CCTGCCTCAG
    GCCATGAACA TCACCTGCAC AGGACGGGGA CCAGACAACT GTATCCAGTG TGCCCACTAC
    ATTGACGGCC CCCACTGCGT CAAGACCTGC CCGGCAGGAG TCATGGGAGA AAACAACACC
    CTGGTCTGGA AGTACGCAGA CGCCGGCCAT GTGTGCCACC TGTGCCATCC AAACTGCACC
    TACGGATGCA CTGGGCCAGG TCTTGAAGGC TGTCCAACGA ATGGGCCTAA GATCCCGTCC
    ATCGCCACTG GGATGGTGGG GGCCCTCCTC TTGCTGCTGG TGGTGGCCCT GGGGATCGGC
    CTCTTCATGC GAAGGCGCCA CATCGTTCGG AAGCGCACGC TGCGGAGGCT GCTGCAGGAG
    AGGGAGCTTG TGGAGCCTCT TACACCCAGT GGAGAAGCTC CCAACCAAGC TCTCTTGAGG
    ATCTTGAAGG AAACTGAATT CAAAAAGATC AAAGTGCTGG GCTCCGGTGC GTTCGGCACG
    GTGTATAAGG GACTCTGGAT CCCAGAAGGT GAGAAAGTTA AAATTCCCGT CGCTATCAAG
    GAATTAAGAG AAGCAACATC TCCGAAAGCC AACAAGGAAA TCCTCGATGA AGCCTACGTG
    ATGGCCAGCG TGGACAACCC CCACGTGTGC CGCCTGCTGG GCATCTGCCT CACCTCCACC
    GTGCAGCTCA TCACGCAGCT CATGCCCTTC GGCTGCCTCC TGGACTATGT CCGGGAACAC
    AAAGACAATA TTGGCTCCCA GTACCTGCTC AACTGGTGTG TGCAGATCGC AAAGGGCATG
    AACTACTTGG AGGACCGTCG CTTGGTGCAC CGCGACCTGG CAGCCAGGAA CGTACTGGTG
    AAAACACCGC AGCATGTCAA GATCACAGAT TTTGGGCTGG CCAAACTGCT GGGTGCGGAA
    GAGAAAGAAT ACCATGCAGA AGGAGGCAAA GTGCCTATCA AGTGGATGGC ATTGGAATCA
    ATTTTACACA GAATCTATAC CCACCAGAGT GATGTCTGGA GCTACGGGGT GACTGTTTGG
    GAGTTGATGA CCTTTGGATC CAAGCCATAT GACGGAATCC CTGCCAGCGA GATCTCCTCC
    ATCCTGGAGA AAGGAGAACG CCTCCCTCAG CCACCCATAT GTACCATCGA TGTCTACATG
    ATCATGGTCA AGTGCTGGAT GATAGACGCA GATAGTCGCC CAAAGTTCCG TGAGTTGATC
    ATCGAATTCT CCAAAATGGC CCGAGACCCC CAGCGCTACC TTGTCATTCA GGGGGATGAA
    AGAATGCATT TGCCAAGTCC TACAGACTCC AACTTCTACC GTGCCCTGAT GGATGAAGAA
    GACATGGACG ACGTGGTGGA TGCCGACGAG TACCTCATCC CACAGCAGGG CTTCTTCAGC
    AGCCCCTCCA CGTCACGGAC TCCCCTCCTG AGCTCTCTGA GTGCAACCAG CAACAATTCC
    ACCGTGGCTT GCATTGATAG AAATGGGCTG CAAAGCTGTC CCATCAAGGA AGACAGCTTC
    TTGCAGCGAT ACAGCTCAGA CCCCACAGGC GCCTTGACTG AGGACAGCAT AGACGACACC
    TTCCTCCCAG TGCCTGGTGA GTGGCTTGTC TGGAAACAGT CCTGCTCCTC AACCTCCTCG
    ACCCACTCAG CAGCAGCCAG TCTCCAGTGT CCAAGCCAGG TGCTCCCTCC AGCATCTCCA
    GAGGGGGAAA CAGTGGCAGA TTTGCAGACA CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333826.1
    CDS258..3533
    Translation

    Target ORF information:

    RefSeq Version NM_001346897.1
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346897.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGGCCAAA AGTGTGATCC AAGCTGTCCC AATGGGAGCT GCTGGGGTGC AGGAGAGGAG
    AACTGCCAGA AACTGACCAA AATCATCTGT GCCCAGCAGT GCTCCGGGCG CTGCCGTGGC
    AAGTCCCCCA GTGACTGCTG CCACAACCAG TGTGCTGCAG GCTGCACAGG CCCCCGGGAG
    AGCGACTGCC TGGTCTGCCG CAAATTCCGA GACGAAGCCA CGTGCAAGGA CACCTGCCCC
    CCACTCATGC TCTACAACCC CACCACGTAC CAGATGGATG TGAACCCCGA GGGCAAATAC
    AGCTTTGGTG CCACCTGCGT GAAGAAGTGT CCCCGTAATT ATGTGGTGAC AGATCACGGC
    TCGTGCGTCC GAGCCTGTGG GGCCGACAGC TATGAGATGG AGGAAGACGG CGTCCGCAAG
    TGTAAGAAGT GCGAAGGGCC TTGCCGCAAA GTGTGTAACG GAATAGGTAT TGGTGAATTT
    AAAGACTCAC TCTCCATAAA TGCTACGAAT ATTAAACACT TCAAAAACTG CACCTCCATC
    AGTGGCGATC TCCACATCCT GCCGGTGGCA TTTAGGGGTG ACTCCTTCAC ACATACTCCT
    CCTCTGGATC CACAGGAACT GGATATTCTG AAAACCGTAA AGGAAATCAC AGGGTTTTTG
    CTGATTCAGG CTTGGCCTGA AAACAGGACG GACCTCCATG CCTTTGAGAA CCTAGAAATC
    ATACGCGGCA GGACCAAGCA ACATGGTCAG TTTTCTCTTG CAGTCGTCAG CCTGAACATA
    ACATCCTTGG GATTACGCTC CCTCAAGGAG ATAAGTGATG GAGATGTGAT AATTTCAGGA
    AACAAAAATT TGTGCTATGC AAATACAATA AACTGGAAAA AACTGTTTGG GACCTCCGGT
    CAGAAAACCA AAATTATAAG CAACAGAGGT GAAAACAGCT GCAAGGCCAC AGGCCAGGTC
    TGCCATGCCT TGTGCTCCCC CGAGGGCTGC TGGGGCCCGG AGCCCAGGGA CTGCGTCTCT
    TGCCGGAATG TCAGCCGAGG CAGGGAATGC GTGGACAAGT GCAACCTTCT GGAGGGTGAG
    CCAAGGGAGT TTGTGGAGAA CTCTGAGTGC ATACAGTGCC ACCCAGAGTG CCTGCCTCAG
    GCCATGAACA TCACCTGCAC AGGACGGGGA CCAGACAACT GTATCCAGTG TGCCCACTAC
    ATTGACGGCC CCCACTGCGT CAAGACCTGC CCGGCAGGAG TCATGGGAGA AAACAACACC
    CTGGTCTGGA AGTACGCAGA CGCCGGCCAT GTGTGCCACC TGTGCCATCC AAACTGCACC
    TACGGATGCA CTGGGCCAGG TCTTGAAGGC TGTCCAACGA ATGGGCCTAA GATCCCGTCC
    ATCGCCACTG GGATGGTGGG GGCCCTCCTC TTGCTGCTGG TGGTGGCCCT GGGGATCGGC
    CTCTTCATGC GAAGGCGCCA CATCGTTCGG AAGCGCACGC TGCGGAGGCT GCTGCAGGAG
    AGGGAGCTTG TGGAGCCTCT TACACCCAGT GGAGAAGCTC CCAACCAAGC TCTCTTGAGG
    ATCTTGAAGG AAACTGAATT CAAAAAGATC AAAGTGCTGG GCTCCGGTGC GTTCGGCACG
    GTGTATAAGG GACTCTGGAT CCCAGAAGGT GAGAAAGTTA AAATTCCCGT CGCTATCAAG
    GAATTAAGAG AAGCAACATC TCCGAAAGCC AACAAGGAAA TCCTCGATGA AGCCTACGTG
    ATGGCCAGCG TGGACAACCC CCACGTGTGC CGCCTGCTGG GCATCTGCCT CACCTCCACC
    GTGCAGCTCA TCACGCAGCT CATGCCCTTC GGCTGCCTCC TGGACTATGT CCGGGAACAC
    AAAGACAATA TTGGCTCCCA GTACCTGCTC AACTGGTGTG TGCAGATCGC AAAGGGCATG
    AACTACTTGG AGGACCGTCG CTTGGTGCAC CGCGACCTGG CAGCCAGGAA CGTACTGGTG
    AAAACACCGC AGCATGTCAA GATCACAGAT TTTGGGCTGG CCAAACTGCT GGGTGCGGAA
    GAGAAAGAAT ACCATGCAGA AGGAGGCAAA GTGCCTATCA AGTGGATGGC ATTGGAATCA
    ATTTTACACA GAATCTATAC CCACCAGAGT GATGTCTGGA GCTACGGGGT GACTGTTTGG
    GAGTTGATGA CCTTTGGATC CAAGCCATAT GACGGAATCC CTGCCAGCGA GATCTCCTCC
    ATCCTGGAGA AAGGAGAACG CCTCCCTCAG CCACCCATAT GTACCATCGA TGTCTACATG
    ATCATGGTCA AGTGCTGGAT GATAGACGCA GATAGTCGCC CAAAGTTCCG TGAGTTGATC
    ATCGAATTCT CCAAAATGGC CCGAGACCCC CAGCGCTACC TTGTCATTCA GGGGGATGAA
    AGAATGCATT TGCCAAGTCC TACAGACTCC AACTTCTACC GTGCCCTGAT GGATGAAGAA
    GACATGGACG ACGTGGTGGA TGCCGACGAG TACCTCATCC CACAGCAGGG CTTCTTCAGC
    AGCCCCTCCA CGTCACGGAC TCCCCTCCTG AGCTCTCTGA GTGCAACCAG CAACAATTCC
    ACCGTGGCTT GCATTGATAG AAATGGGCTG CAAAGCTGTC CCATCAAGGA AGACAGCTTC
    TTGCAGCGAT ACAGCTCAGA CCCCACAGGC GCCTTGACTG AGGACAGCAT AGACGACACC
    TTCCTCCCAG TGCCTGGTGA GTGGCTTGTC TGGAAACAGT CCTGCTCCTC AACCTCCTCG
    ACCCACTCAG CAGCAGCCAG TCTCCAGTGT CCAAGCCAGG TGCTCCCTCC AGCATCTCCA
    GAGGGGGAAA CAGTGGCAGA TTTGCAGACA CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102780
    Clone ID Related Accession (Same CDS sequence) NM_001346897.1 , NM_001346897.2
    Accession Version NM_001346897.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Homo sapiens(human)
    Product epidermal growth factor receptor isoform e precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006977.3, BC094761.1 and AC073324.6. On May 31, 2019 this sequence version replaced NM_001346897.1. Transcript Variant: This variant (5) lacks an in-frame exon in the 5' coding region and its 3' terminal exon extends past a splice site that is used in variant 1. The encoded isoform (e) is shorter and has a distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC094761.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153307, SAMN03267770 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGGCCAAA AGTGTGATCC AAGCTGTCCC AATGGGAGCT GCTGGGGTGC AGGAGAGGAG
    AACTGCCAGA AACTGACCAA AATCATCTGT GCCCAGCAGT GCTCCGGGCG CTGCCGTGGC
    AAGTCCCCCA GTGACTGCTG CCACAACCAG TGTGCTGCAG GCTGCACAGG CCCCCGGGAG
    AGCGACTGCC TGGTCTGCCG CAAATTCCGA GACGAAGCCA CGTGCAAGGA CACCTGCCCC
    CCACTCATGC TCTACAACCC CACCACGTAC CAGATGGATG TGAACCCCGA GGGCAAATAC
    AGCTTTGGTG CCACCTGCGT GAAGAAGTGT CCCCGTAATT ATGTGGTGAC AGATCACGGC
    TCGTGCGTCC GAGCCTGTGG GGCCGACAGC TATGAGATGG AGGAAGACGG CGTCCGCAAG
    TGTAAGAAGT GCGAAGGGCC TTGCCGCAAA GTGTGTAACG GAATAGGTAT TGGTGAATTT
    AAAGACTCAC TCTCCATAAA TGCTACGAAT ATTAAACACT TCAAAAACTG CACCTCCATC
    AGTGGCGATC TCCACATCCT GCCGGTGGCA TTTAGGGGTG ACTCCTTCAC ACATACTCCT
    CCTCTGGATC CACAGGAACT GGATATTCTG AAAACCGTAA AGGAAATCAC AGGGTTTTTG
    CTGATTCAGG CTTGGCCTGA AAACAGGACG GACCTCCATG CCTTTGAGAA CCTAGAAATC
    ATACGCGGCA GGACCAAGCA ACATGGTCAG TTTTCTCTTG CAGTCGTCAG CCTGAACATA
    ACATCCTTGG GATTACGCTC CCTCAAGGAG ATAAGTGATG GAGATGTGAT AATTTCAGGA
    AACAAAAATT TGTGCTATGC AAATACAATA AACTGGAAAA AACTGTTTGG GACCTCCGGT
    CAGAAAACCA AAATTATAAG CAACAGAGGT GAAAACAGCT GCAAGGCCAC AGGCCAGGTC
    TGCCATGCCT TGTGCTCCCC CGAGGGCTGC TGGGGCCCGG AGCCCAGGGA CTGCGTCTCT
    TGCCGGAATG TCAGCCGAGG CAGGGAATGC GTGGACAAGT GCAACCTTCT GGAGGGTGAG
    CCAAGGGAGT TTGTGGAGAA CTCTGAGTGC ATACAGTGCC ACCCAGAGTG CCTGCCTCAG
    GCCATGAACA TCACCTGCAC AGGACGGGGA CCAGACAACT GTATCCAGTG TGCCCACTAC
    ATTGACGGCC CCCACTGCGT CAAGACCTGC CCGGCAGGAG TCATGGGAGA AAACAACACC
    CTGGTCTGGA AGTACGCAGA CGCCGGCCAT GTGTGCCACC TGTGCCATCC AAACTGCACC
    TACGGATGCA CTGGGCCAGG TCTTGAAGGC TGTCCAACGA ATGGGCCTAA GATCCCGTCC
    ATCGCCACTG GGATGGTGGG GGCCCTCCTC TTGCTGCTGG TGGTGGCCCT GGGGATCGGC
    CTCTTCATGC GAAGGCGCCA CATCGTTCGG AAGCGCACGC TGCGGAGGCT GCTGCAGGAG
    AGGGAGCTTG TGGAGCCTCT TACACCCAGT GGAGAAGCTC CCAACCAAGC TCTCTTGAGG
    ATCTTGAAGG AAACTGAATT CAAAAAGATC AAAGTGCTGG GCTCCGGTGC GTTCGGCACG
    GTGTATAAGG GACTCTGGAT CCCAGAAGGT GAGAAAGTTA AAATTCCCGT CGCTATCAAG
    GAATTAAGAG AAGCAACATC TCCGAAAGCC AACAAGGAAA TCCTCGATGA AGCCTACGTG
    ATGGCCAGCG TGGACAACCC CCACGTGTGC CGCCTGCTGG GCATCTGCCT CACCTCCACC
    GTGCAGCTCA TCACGCAGCT CATGCCCTTC GGCTGCCTCC TGGACTATGT CCGGGAACAC
    AAAGACAATA TTGGCTCCCA GTACCTGCTC AACTGGTGTG TGCAGATCGC AAAGGGCATG
    AACTACTTGG AGGACCGTCG CTTGGTGCAC CGCGACCTGG CAGCCAGGAA CGTACTGGTG
    AAAACACCGC AGCATGTCAA GATCACAGAT TTTGGGCTGG CCAAACTGCT GGGTGCGGAA
    GAGAAAGAAT ACCATGCAGA AGGAGGCAAA GTGCCTATCA AGTGGATGGC ATTGGAATCA
    ATTTTACACA GAATCTATAC CCACCAGAGT GATGTCTGGA GCTACGGGGT GACTGTTTGG
    GAGTTGATGA CCTTTGGATC CAAGCCATAT GACGGAATCC CTGCCAGCGA GATCTCCTCC
    ATCCTGGAGA AAGGAGAACG CCTCCCTCAG CCACCCATAT GTACCATCGA TGTCTACATG
    ATCATGGTCA AGTGCTGGAT GATAGACGCA GATAGTCGCC CAAAGTTCCG TGAGTTGATC
    ATCGAATTCT CCAAAATGGC CCGAGACCCC CAGCGCTACC TTGTCATTCA GGGGGATGAA
    AGAATGCATT TGCCAAGTCC TACAGACTCC AACTTCTACC GTGCCCTGAT GGATGAAGAA
    GACATGGACG ACGTGGTGGA TGCCGACGAG TACCTCATCC CACAGCAGGG CTTCTTCAGC
    AGCCCCTCCA CGTCACGGAC TCCCCTCCTG AGCTCTCTGA GTGCAACCAG CAACAATTCC
    ACCGTGGCTT GCATTGATAG AAATGGGCTG CAAAGCTGTC CCATCAAGGA AGACAGCTTC
    TTGCAGCGAT ACAGCTCAGA CCCCACAGGC GCCTTGACTG AGGACAGCAT AGACGACACC
    TTCCTCCCAG TGCCTGGTGA GTGGCTTGTC TGGAAACAGT CCTGCTCCTC AACCTCCTCG
    ACCCACTCAG CAGCAGCCAG TCTCCAGTGT CCAAGCCAGG TGCTCCCTCC AGCATCTCCA
    GAGGGGGAAA CAGTGGCAGA TTTGCAGACA CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333826.1
    CDS262..3537
    Translation

    Target ORF information:

    RefSeq Version NM_001346897.2
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346897.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
    GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
    TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
    GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
    ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
    TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
    GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
    CAGGGCCAAA AGTGTGATCC AAGCTGTCCC AATGGGAGCT GCTGGGGTGC AGGAGAGGAG
    AACTGCCAGA AACTGACCAA AATCATCTGT GCCCAGCAGT GCTCCGGGCG CTGCCGTGGC
    AAGTCCCCCA GTGACTGCTG CCACAACCAG TGTGCTGCAG GCTGCACAGG CCCCCGGGAG
    AGCGACTGCC TGGTCTGCCG CAAATTCCGA GACGAAGCCA CGTGCAAGGA CACCTGCCCC
    CCACTCATGC TCTACAACCC CACCACGTAC CAGATGGATG TGAACCCCGA GGGCAAATAC
    AGCTTTGGTG CCACCTGCGT GAAGAAGTGT CCCCGTAATT ATGTGGTGAC AGATCACGGC
    TCGTGCGTCC GAGCCTGTGG GGCCGACAGC TATGAGATGG AGGAAGACGG CGTCCGCAAG
    TGTAAGAAGT GCGAAGGGCC TTGCCGCAAA GTGTGTAACG GAATAGGTAT TGGTGAATTT
    AAAGACTCAC TCTCCATAAA TGCTACGAAT ATTAAACACT TCAAAAACTG CACCTCCATC
    AGTGGCGATC TCCACATCCT GCCGGTGGCA TTTAGGGGTG ACTCCTTCAC ACATACTCCT
    CCTCTGGATC CACAGGAACT GGATATTCTG AAAACCGTAA AGGAAATCAC AGGGTTTTTG
    CTGATTCAGG CTTGGCCTGA AAACAGGACG GACCTCCATG CCTTTGAGAA CCTAGAAATC
    ATACGCGGCA GGACCAAGCA ACATGGTCAG TTTTCTCTTG CAGTCGTCAG CCTGAACATA
    ACATCCTTGG GATTACGCTC CCTCAAGGAG ATAAGTGATG GAGATGTGAT AATTTCAGGA
    AACAAAAATT TGTGCTATGC AAATACAATA AACTGGAAAA AACTGTTTGG GACCTCCGGT
    CAGAAAACCA AAATTATAAG CAACAGAGGT GAAAACAGCT GCAAGGCCAC AGGCCAGGTC
    TGCCATGCCT TGTGCTCCCC CGAGGGCTGC TGGGGCCCGG AGCCCAGGGA CTGCGTCTCT
    TGCCGGAATG TCAGCCGAGG CAGGGAATGC GTGGACAAGT GCAACCTTCT GGAGGGTGAG
    CCAAGGGAGT TTGTGGAGAA CTCTGAGTGC ATACAGTGCC ACCCAGAGTG CCTGCCTCAG
    GCCATGAACA TCACCTGCAC AGGACGGGGA CCAGACAACT GTATCCAGTG TGCCCACTAC
    ATTGACGGCC CCCACTGCGT CAAGACCTGC CCGGCAGGAG TCATGGGAGA AAACAACACC
    CTGGTCTGGA AGTACGCAGA CGCCGGCCAT GTGTGCCACC TGTGCCATCC AAACTGCACC
    TACGGATGCA CTGGGCCAGG TCTTGAAGGC TGTCCAACGA ATGGGCCTAA GATCCCGTCC
    ATCGCCACTG GGATGGTGGG GGCCCTCCTC TTGCTGCTGG TGGTGGCCCT GGGGATCGGC
    CTCTTCATGC GAAGGCGCCA CATCGTTCGG AAGCGCACGC TGCGGAGGCT GCTGCAGGAG
    AGGGAGCTTG TGGAGCCTCT TACACCCAGT GGAGAAGCTC CCAACCAAGC TCTCTTGAGG
    ATCTTGAAGG AAACTGAATT CAAAAAGATC AAAGTGCTGG GCTCCGGTGC GTTCGGCACG
    GTGTATAAGG GACTCTGGAT CCCAGAAGGT GAGAAAGTTA AAATTCCCGT CGCTATCAAG
    GAATTAAGAG AAGCAACATC TCCGAAAGCC AACAAGGAAA TCCTCGATGA AGCCTACGTG
    ATGGCCAGCG TGGACAACCC CCACGTGTGC CGCCTGCTGG GCATCTGCCT CACCTCCACC
    GTGCAGCTCA TCACGCAGCT CATGCCCTTC GGCTGCCTCC TGGACTATGT CCGGGAACAC
    AAAGACAATA TTGGCTCCCA GTACCTGCTC AACTGGTGTG TGCAGATCGC AAAGGGCATG
    AACTACTTGG AGGACCGTCG CTTGGTGCAC CGCGACCTGG CAGCCAGGAA CGTACTGGTG
    AAAACACCGC AGCATGTCAA GATCACAGAT TTTGGGCTGG CCAAACTGCT GGGTGCGGAA
    GAGAAAGAAT ACCATGCAGA AGGAGGCAAA GTGCCTATCA AGTGGATGGC ATTGGAATCA
    ATTTTACACA GAATCTATAC CCACCAGAGT GATGTCTGGA GCTACGGGGT GACTGTTTGG
    GAGTTGATGA CCTTTGGATC CAAGCCATAT GACGGAATCC CTGCCAGCGA GATCTCCTCC
    ATCCTGGAGA AAGGAGAACG CCTCCCTCAG CCACCCATAT GTACCATCGA TGTCTACATG
    ATCATGGTCA AGTGCTGGAT GATAGACGCA GATAGTCGCC CAAAGTTCCG TGAGTTGATC
    ATCGAATTCT CCAAAATGGC CCGAGACCCC CAGCGCTACC TTGTCATTCA GGGGGATGAA
    AGAATGCATT TGCCAAGTCC TACAGACTCC AACTTCTACC GTGCCCTGAT GGATGAAGAA
    GACATGGACG ACGTGGTGGA TGCCGACGAG TACCTCATCC CACAGCAGGG CTTCTTCAGC
    AGCCCCTCCA CGTCACGGAC TCCCCTCCTG AGCTCTCTGA GTGCAACCAG CAACAATTCC
    ACCGTGGCTT GCATTGATAG AAATGGGCTG CAAAGCTGTC CCATCAAGGA AGACAGCTTC
    TTGCAGCGAT ACAGCTCAGA CCCCACAGGC GCCTTGACTG AGGACAGCAT AGACGACACC
    TTCCTCCCAG TGCCTGGTGA GTGGCTTGTC TGGAAACAGT CCTGCTCCTC AACCTCCTCG
    ACCCACTCAG CAGCAGCCAG TCTCCAGTGT CCAAGCCAGG TGCTCCCTCC AGCATCTCCA
    GAGGGGGAAA CAGTGGCAGA TTTGCAGACA CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.