EIF4G1 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following EIF4G1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF4G1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu13769 NM_182917.4
Latest version!
Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$659.50-$923.30
$1319.00
OHu13773 NM_198242.3
Latest version!
Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu13775 NM_001194946.1
Latest version!
Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu13775 NM_001194947.1
Latest version!
Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu13776 NM_004953.5
Latest version!
Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu18102 NM_198241.2
Latest version!
Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$659.50-$923.30
$1319.00
OHu108646 NM_001194946.2
Latest version!
Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu108651 NM_198241.3
Latest version!
Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu108653 NM_001291157.2
Latest version!
Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu108656 NM_198244.3
Latest version!
Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu13778 NM_198244.2
Latest version!
Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $384.30
$549.00
OHu15300 NM_001291157.1
Latest version!
Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
View Old Accession Versions >>
OHu13776 NM_004953.4 Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu13773 NM_198242.2 Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu13769
    Clone ID Related Accession (Same CDS sequence) NM_182917.4
    Accession Version NM_182917.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-20
    Organism Homo sapiens(human)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC347118.1, AY082886.1, AF002815.1, AK308319.1 and BQ011010.1. On Aug 13, 2010 this sequence version replaced NM_182917.3. Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF281070.1, AY082886.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 12052860 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGTC CACAGGCCCC CCACCCGCCC CATCCCCCGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCGCCG GTGGTGTTCA GTACGCCACA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG
    GGGGCCTACT ACATCCCTGG ACAGGGGCGT TCCACATACG TTGTCCCGAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACAGA ATTTGGGACC
    TACGCTGGCG CCTACTATCC AGCCCAAGGG GTGCAGCAGT TTCCCACTGG CGTGGCCCCC
    GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG TAAGACGATC
    CGAATTCGAG ATCCAAACCA AGGAGGAAAG GATATCACAG AGGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC CCCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAAGCTAAT
    GGGGAGACGC CCCAGGTTGC TGTCATTGTC CGGCCAGATG ACCGGTCACA GGGAGCAATC
    ATTGCTGACC GGCCAGGGCT GCCTGGCCCA GAGCATAGCC CTTCAGAATC CCAGCCTTCG
    TCGCCTTCTC CGACCCCATC ACCATCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC
    GCAGTCCTCT CTATTCCTGG GGACACTATG ACAACTATAC AAATGTCTGT AGAAGAATCA
    ACCCCCATCT CCCGTGAAAC TGGGGAGCCA TATCGCCTCT CTCCAGAACC CACTCCTCTC
    GCCGAACCCA TACTGGAAGT AGAAGTGACA CTTAGCAAAC CGGTTCCAGA ATCTGAGTTT
    TCTTCCAGTC CTCTCCAGGC TCCCACCCCT TTGGCATCTC ACACAGTGGA AATTCATGAG
    CCTAATGGCA TGGTCCCATC TGAAGATCTG GAACCAGAGG TGGAGTCAAG CCCAGAGCTT
    GCTCCTCCCC CAGCTTGCCC CTCCGAATCC CCTGTGCCCA TTGCTCCAAC TGCCCAACCT
    GAGGAACTGC TCAACGGAGC CCCCTCGCCA CCAGCTGTGG ACTTAAGCCC AGTCAGTGAG
    CCAGAGGAGC AGGCCAAGGA GGTGACAGCA TCAATGGCGC CCCCCACCAT CCCCTCTGCT
    ACTCCAGCTA CGGCTCCTTC AGCTACTTCC CCAGCTCAGG AGGAGGAAAT GGAAGAAGAA
    GAAGAAGAGG AAGAAGGAGA AGCAGGAGAA GCAGGAGAAG CTGAGAGTGA GAAAGGAGGA
    GAGGAACTGC TCCCCCCAGA GAGTACCCCT ATTCCAGCCA ACTTGTCTCA GAATTTGGAG
    GCAGCAGCAG CCACTCAAGT GGCAGTATCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG
    CTAAATAAGA AGGAGGCTGT TGGAGACCTT CTGGATGCCT TCAAGGAGGC GAACCCGGCA
    GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCAGCAATC CAGGCCCAGA GTCTGAGGGC
    AGTGGTGTGC CCCCACGTCC TGAGGAAGCA GATGAGACCT GGGACTCAAA GGAAGACAAA
    ATTCACAATG CTGAGAACAT CCAGCCCGGG GAACAGAAGT ATGAATATAA GTCAGATCAG
    TGGAAGCCTC TAAACCTAGA GGAGAAAAAA CGTTACGACC GTGAGTTCCT GCTTGGTTTT
    CAGTTCATCT TTGCCAGTAT GCAGAAGCCA GAGGGATTGC CACATATCAG TGACGTGGTG
    CTGGACAAGG CCAATAAAAC ACCACTGCGG CCACTGGATC CCACTAGACT ACAAGGCATA
    AATTGTGGCC CAGACTTCAC TCCATCCTTT GCCAACCTTG GCCGGACAAC CCTTAGCACC
    CGTGGGCCCC CAAGGGGTGG GCCAGGTGGG GAGCTGCCCC GTGGGCCGCA GGCTGGCCTG
    GGACCCCGGC GCTCTCAGCA GGGACCCCGA AAAGAACCAC GCAAGATCAT TGCCACAGTG
    TTAATGACCG AAGATATAAA ACTGAACAAA GCAGAGAAAG CCTGGAAACC CAGCAGCAAG
    CGGACGGCGG CTGATAAGGA TCGAGGGGAA GAAGATGCTG ATGGCAGCAA AACCCAGGAC
    CTATTCCGCA GGGTGCGCTC CATCCTGAAT AAACTGACAC CCCAGATGTT CCAGCAGCTG
    ATGAAGCAAG TGACGCAGCT GGCCATCGAC ACCGAGGAAC GCCTCAAAGG GGTCATTGAC
    CTCATTTTTG AGAAGGCCAT TTCAGAGCCC AACTTCTCTG TGGCCTATGC CAACATGTGC
    CGCTGCCTCA TGGCGCTGAA AGTGCCCACT ACGGAAAAGC CAACAGTGAC TGTGAACTTC
    CGAAAGCTGT TGTTGAATCG ATGTCAGAAG GAGTTTGAGA AAGACAAAGA TGATGATGAG
    GTTTTTGAGA AGAAGCAAAA AGAGATGGAT GAAGCTGCTA CGGCAGAGGA ACGAGGACGC
    CTGAAGGAAG AGCTGGAAGA GGCTCGGGAC ATAGCCCGGC GGCGCTCTTT AGGGAATATC
    AAGTTTATTG GAGAGTTGTT CAAACTGAAG ATGTTAACAG AGGCAATAAT GCATGACTGT
    GTGGTCAAAC TGCTTAAGAA CCATGATGAA GAGTCCCTTG AGTGCCTTTG TCGTCTGCTC
    ACCACCATTG GCAAAGACCT GGACTTTGAA AAAGCCAAGC CCCGAATGGA TCAGTATTTC
    AACCAGATGG AAAAAATCAT TAAAGAAAAG AAGACGTCAT CCCGCATCCG CTTTATGCTG
    CAGGACGTGC TGGATCTGCG AGGGAGCAAT TGGGTGCCAC GCCGAGGGGA TCAGGGTCCC
    AAGACCATTG ACCAGATCCA TAAGGAGGCT GAGATGGAAG AACATCGAGA GCACATCAAA
    GTGCAGCAGC TCATGGCCAA GGGCAGTGAC AAGCGTCGGG GCGGTCCTCC AGGCCCTCCC
    ATCAGCCGTG GACTTCCCCT TGTGGATGAT GGTGGCTGGA ACACAGTTCC CATCAGCAAA
    GGTAGCCGCC CCATTGACAC CTCACGACTC ACCAAGATCA CCAAGCCTGG CTCCATCGAT
    TCTAACAACC AGCTCTTTGC ACCTGGAGGG CGACTGAGCT GGGGCAAGGG CAGCAGCGGA
    GGCTCAGGAG CCAAGCCCTC AGACGCAGCA TCAGAAGCTG CTCGCCCAGC TACTAGTACT
    TTGAATCGCT TCTCAGCCCT TCAACAAGCG GTACCCACAG AAAGCACAGA TAATAGACGT
    GTGGTGCAGA GGAGTAGCTT GAGCCGAGAA CGAGGCGAGA AAGCTGGAGA CCGAGGAGAC
    CGCCTAGAGC GGAGTGAACG GGGAGGGGAC CGTGGGGACC GGCTTGATCG TGCGCGGACA
    CCTGCTACCA AGCGGAGCTT CAGCAAGGAA GTGGAGGAGC GGAGTAGAGA ACGGCCCTCC
    CAGCCTGAGG GGCTGCGCAA GGCAGCTAGC CTCACGGAGG ATCGGGACCG TGGGCGGGAT
    GCCGTGAAGC GAGAAGCTGC CCTACCCCCA GTGAGCCCCC TGAAGGCGGC TCTCTCTGAG
    GAGGAGTTAG AGAAGAAATC CAAGGCTATC ATTGAGGAAT ATCTCCATCT CAATGACATG
    AAAGAGGCAG TCCAGTGCGT GCAGGAGCTG GCCTCACCCT CCTTGCTCTT CATCTTTGTA
    CGGCATGGTG TCGAGTCTAC GCTGGAGCGC AGTGCCATTG CTCGTGAGCA TATGGGGCAG
    CTGCTGCACC AGCTGCTCTG TGCTGGGCAT CTGTCTACTG CTCAGTACTA CCAAGGGTTG
    TATGAAATCT TGGAATTGGC TGAGGACATG GAAATTGACA TCCCCCACGT GTGGCTCTAC
    CTAGCGGAAC TGGTAACACC CATTCTGCAG GAAGGTGGGG TGCCCATGGG GGAGCTGTTC
    AGGGAGATTA CAAAGCCTCT GAGACCGTTG GGCAAAGCTG CTTCCCTGTT GCTGGAGATC
    CTGGGCCTCC TGTGCAAAAG CATGGGTCCT AAAAAGGTGG GGACGCTGTG GCGAGAAGCC
    GGGCTTAGCT GGAAGGAATT TCTACCTGAA GGCCAGGACA TTGGTGCATT CGTCGCTGAA
    CAGAAGGTGG AGTATACCCT GGGAGAGGAG TCGGAAGCCC CTGGCCAGAG GGCACTCCCC
    TCCGAGGAGC TGAACAGGCA GCTGGAGAAG CTGCTGAAGG AGGGCAGCAG TAACCAGCGG
    GTGTTCGACT GGATAGAGGC CAACCTGAGT GAGCAGCAGA TAGTATCCAA CACGTTAGTT
    CGAGCCCTCA TGACGGCTGT CTGCTATTCT GCAATTATTT TTGAGACTCC CCTCCGAGTG
    GACGTTGCAG TGCTGAAAGC GCGAGCGAAG CTGCTGCAGA AATACCTGTG TGACGAGCAG
    AAGGAGCTAC AGGCGCTCTA CGCCCTCCAG GCCCTTGTAG TGACCTTAGA ACAGCCTCCC
    AACCTGCTGC GGATGTTCTT TGACGCACTG TATGACGAGG ACGTGGTGAA GGAGGATGCC
    TTCTACAGTT GGGAGAGTAG CAAGGACCCC GCTGAGCAGC AGGGCAAGGG TGTGGCCCTT
    AAATCTGTCA CAGCCTTCTT CAAGTGGCTC CGTGAAGCAG AGGAGGAGTC TGACCACAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_886553.3
    CDS417..5219
    Translation

    Target ORF information:

    RefSeq Version NM_182917.4
    Organism Homo sapiens(human)
    Definition Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_182917.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGTC CACAGGCCCC CCACCCGCCC CATCCCCCGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCGCCG GTGGTGTTCA GTACGCCACA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG
    GGGGCCTACT ACATCCCTGG ACAGGGGCGT TCCACATACG TTGTCCCGAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACAGA ATTTGGGACC
    TACGCTGGCG CCTACTATCC AGCCCAAGGG GTGCAGCAGT TTCCCACTGG CGTGGCCCCC
    GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG TAAGACGATC
    CGAATTCGAG ATCCAAACCA AGGAGGAAAG GATATCACAG AGGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC CCCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAAGCTAAT
    GGGGAGACGC CCCAGGTTGC TGTCATTGTC CGGCCAGATG ACCGGTCACA GGGAGCAATC
    ATTGCTGACC GGCCAGGGCT GCCTGGCCCA GAGCATAGCC CTTCAGAATC CCAGCCTTCG
    TCGCCTTCTC CGACCCCATC ACCATCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC
    GCAGTCCTCT CTATTCCTGG GGACACTATG ACAACTATAC AAATGTCTGT AGAAGAATCA
    ACCCCCATCT CCCGTGAAAC TGGGGAGCCA TATCGCCTCT CTCCAGAACC CACTCCTCTC
    GCCGAACCCA TACTGGAAGT AGAAGTGACA CTTAGCAAAC CGGTTCCAGA ATCTGAGTTT
    TCTTCCAGTC CTCTCCAGGC TCCCACCCCT TTGGCATCTC ACACAGTGGA AATTCATGAG
    CCTAATGGCA TGGTCCCATC TGAAGATCTG GAACCAGAGG TGGAGTCAAG CCCAGAGCTT
    GCTCCTCCCC CAGCTTGCCC CTCCGAATCC CCTGTGCCCA TTGCTCCAAC TGCCCAACCT
    GAGGAACTGC TCAACGGAGC CCCCTCGCCA CCAGCTGTGG ACTTAAGCCC AGTCAGTGAG
    CCAGAGGAGC AGGCCAAGGA GGTGACAGCA TCAATGGCGC CCCCCACCAT CCCCTCTGCT
    ACTCCAGCTA CGGCTCCTTC AGCTACTTCC CCAGCTCAGG AGGAGGAAAT GGAAGAAGAA
    GAAGAAGAGG AAGAAGGAGA AGCAGGAGAA GCAGGAGAAG CTGAGAGTGA GAAAGGAGGA
    GAGGAACTGC TCCCCCCAGA GAGTACCCCT ATTCCAGCCA ACTTGTCTCA GAATTTGGAG
    GCAGCAGCAG CCACTCAAGT GGCAGTATCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG
    CTAAATAAGA AGGAGGCTGT TGGAGACCTT CTGGATGCCT TCAAGGAGGC GAACCCGGCA
    GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCAGCAATC CAGGCCCAGA GTCTGAGGGC
    AGTGGTGTGC CCCCACGTCC TGAGGAAGCA GATGAGACCT GGGACTCAAA GGAAGACAAA
    ATTCACAATG CTGAGAACAT CCAGCCCGGG GAACAGAAGT ATGAATATAA GTCAGATCAG
    TGGAAGCCTC TAAACCTAGA GGAGAAAAAA CGTTACGACC GTGAGTTCCT GCTTGGTTTT
    CAGTTCATCT TTGCCAGTAT GCAGAAGCCA GAGGGATTGC CACATATCAG TGACGTGGTG
    CTGGACAAGG CCAATAAAAC ACCACTGCGG CCACTGGATC CCACTAGACT ACAAGGCATA
    AATTGTGGCC CAGACTTCAC TCCATCCTTT GCCAACCTTG GCCGGACAAC CCTTAGCACC
    CGTGGGCCCC CAAGGGGTGG GCCAGGTGGG GAGCTGCCCC GTGGGCCGCA GGCTGGCCTG
    GGACCCCGGC GCTCTCAGCA GGGACCCCGA AAAGAACCAC GCAAGATCAT TGCCACAGTG
    TTAATGACCG AAGATATAAA ACTGAACAAA GCAGAGAAAG CCTGGAAACC CAGCAGCAAG
    CGGACGGCGG CTGATAAGGA TCGAGGGGAA GAAGATGCTG ATGGCAGCAA AACCCAGGAC
    CTATTCCGCA GGGTGCGCTC CATCCTGAAT AAACTGACAC CCCAGATGTT CCAGCAGCTG
    ATGAAGCAAG TGACGCAGCT GGCCATCGAC ACCGAGGAAC GCCTCAAAGG GGTCATTGAC
    CTCATTTTTG AGAAGGCCAT TTCAGAGCCC AACTTCTCTG TGGCCTATGC CAACATGTGC
    CGCTGCCTCA TGGCGCTGAA AGTGCCCACT ACGGAAAAGC CAACAGTGAC TGTGAACTTC
    CGAAAGCTGT TGTTGAATCG ATGTCAGAAG GAGTTTGAGA AAGACAAAGA TGATGATGAG
    GTTTTTGAGA AGAAGCAAAA AGAGATGGAT GAAGCTGCTA CGGCAGAGGA ACGAGGACGC
    CTGAAGGAAG AGCTGGAAGA GGCTCGGGAC ATAGCCCGGC GGCGCTCTTT AGGGAATATC
    AAGTTTATTG GAGAGTTGTT CAAACTGAAG ATGTTAACAG AGGCAATAAT GCATGACTGT
    GTGGTCAAAC TGCTTAAGAA CCATGATGAA GAGTCCCTTG AGTGCCTTTG TCGTCTGCTC
    ACCACCATTG GCAAAGACCT GGACTTTGAA AAAGCCAAGC CCCGAATGGA TCAGTATTTC
    AACCAGATGG AAAAAATCAT TAAAGAAAAG AAGACGTCAT CCCGCATCCG CTTTATGCTG
    CAGGACGTGC TGGATCTGCG AGGGAGCAAT TGGGTGCCAC GCCGAGGGGA TCAGGGTCCC
    AAGACCATTG ACCAGATCCA TAAGGAGGCT GAGATGGAAG AACATCGAGA GCACATCAAA
    GTGCAGCAGC TCATGGCCAA GGGCAGTGAC AAGCGTCGGG GCGGTCCTCC AGGCCCTCCC
    ATCAGCCGTG GACTTCCCCT TGTGGATGAT GGTGGCTGGA ACACAGTTCC CATCAGCAAA
    GGTAGCCGCC CCATTGACAC CTCACGACTC ACCAAGATCA CCAAGCCTGG CTCCATCGAT
    TCTAACAACC AGCTCTTTGC ACCTGGAGGG CGACTGAGCT GGGGCAAGGG CAGCAGCGGA
    GGCTCAGGAG CCAAGCCCTC AGACGCAGCA TCAGAAGCTG CTCGCCCAGC TACTAGTACT
    TTGAATCGCT TCTCAGCCCT TCAACAAGCG GTACCCACAG AAAGCACAGA TAATAGACGT
    GTGGTGCAGA GGAGTAGCTT GAGCCGAGAA CGAGGCGAGA AAGCTGGAGA CCGAGGAGAC
    CGCCTAGAGC GGAGTGAACG GGGAGGGGAC CGTGGGGACC GGCTTGATCG TGCGCGGACA
    CCTGCTACCA AGCGGAGCTT CAGCAAGGAA GTGGAGGAGC GGAGTAGAGA ACGGCCCTCC
    CAGCCTGAGG GGCTGCGCAA GGCAGCTAGC CTCACGGAGG ATCGGGACCG TGGGCGGGAT
    GCCGTGAAGC GAGAAGCTGC CCTACCCCCA GTGAGCCCCC TGAAGGCGGC TCTCTCTGAG
    GAGGAGTTAG AGAAGAAATC CAAGGCTATC ATTGAGGAAT ATCTCCATCT CAATGACATG
    AAAGAGGCAG TCCAGTGCGT GCAGGAGCTG GCCTCACCCT CCTTGCTCTT CATCTTTGTA
    CGGCATGGTG TCGAGTCTAC GCTGGAGCGC AGTGCCATTG CTCGTGAGCA TATGGGGCAG
    CTGCTGCACC AGCTGCTCTG TGCTGGGCAT CTGTCTACTG CTCAGTACTA CCAAGGGTTG
    TATGAAATCT TGGAATTGGC TGAGGACATG GAAATTGACA TCCCCCACGT GTGGCTCTAC
    CTAGCGGAAC TGGTAACACC CATTCTGCAG GAAGGTGGGG TGCCCATGGG GGAGCTGTTC
    AGGGAGATTA CAAAGCCTCT GAGACCGTTG GGCAAAGCTG CTTCCCTGTT GCTGGAGATC
    CTGGGCCTCC TGTGCAAAAG CATGGGTCCT AAAAAGGTGG GGACGCTGTG GCGAGAAGCC
    GGGCTTAGCT GGAAGGAATT TCTACCTGAA GGCCAGGACA TTGGTGCATT CGTCGCTGAA
    CAGAAGGTGG AGTATACCCT GGGAGAGGAG TCGGAAGCCC CTGGCCAGAG GGCACTCCCC
    TCCGAGGAGC TGAACAGGCA GCTGGAGAAG CTGCTGAAGG AGGGCAGCAG TAACCAGCGG
    GTGTTCGACT GGATAGAGGC CAACCTGAGT GAGCAGCAGA TAGTATCCAA CACGTTAGTT
    CGAGCCCTCA TGACGGCTGT CTGCTATTCT GCAATTATTT TTGAGACTCC CCTCCGAGTG
    GACGTTGCAG TGCTGAAAGC GCGAGCGAAG CTGCTGCAGA AATACCTGTG TGACGAGCAG
    AAGGAGCTAC AGGCGCTCTA CGCCCTCCAG GCCCTTGTAG TGACCTTAGA ACAGCCTCCC
    AACCTGCTGC GGATGTTCTT TGACGCACTG TATGACGAGG ACGTGGTGAA GGAGGATGCC
    TTCTACAGTT GGGAGAGTAG CAAGGACCCC GCTGAGCAGC AGGGCAAGGG TGTGGCCCTT
    AAATCTGTCA CAGCCTTCTT CAAGTGGCTC CGTGAAGCAG AGGAGGAGTC TGACCACAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13773
    Clone ID Related Accession (Same CDS sequence) NM_198242.2 , NM_198242.3
    Accession Version NM_198242.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR980540.1, AL832754.1, BM475154.1, CX165113.1 and BQ011010.1. On Aug 13, 2010 this sequence version replaced NM_198242.1. Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, uses a downstream start codon, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (3) is shorter at the N-terminus and lacks an internal residue, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.86057.1, AL832754.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAACCAGC CACCCCAGAT TGCTCCCAAG AGGGAGCGTA AGACGATCCG AATTCGAGAT 
    CCAAACCAAG GAGGAAAGGA TATCACAGAG GAGATCATGT CTGGGGCCCG CACTGCCTCC
    ACACCCACCC CTCCCCAGAC GGGAGGCGGT CTGGAGCCTC AAGCTAATGG GGAGACGCCC
    CAGGTTGCTG TCATTGTCCG GCCAGATGAC CGGTCACAGG GAGCAATCAT TGCTGACCGG
    CCAGGGCTGC CTGGCCCAGA GCATAGCCCT TCAGAATCCC AGCCTTCGTC GCCTTCTCCG
    ACCCCATCAC CATCCCCAGT CTTGGAACCG GGGTCTGAGC CTAATCTCGC AGTCCTCTCT
    ATTCCTGGGG ACACTATGAC AACTATACAA ATGTCTGTAG AAGAATCAAC CCCCATCTCC
    CGTGAAACTG GGGAGCCATA TCGCCTCTCT CCAGAACCCA CTCCTCTCGC CGAACCCATA
    CTGGAAGTAG AAGTGACACT TAGCAAACCG GTTCCAGAAT CTGAGTTTTC TTCCAGTCCT
    CTCCAGGCTC CCACCCCTTT GGCATCTCAC ACAGTGGAAA TTCATGAGCC TAATGGCATG
    GTCCCATCTG AAGATCTGGA ACCAGAGGTG GAGTCAAGCC CAGAGCTTGC TCCTCCCCCA
    GCTTGCCCCT CCGAATCCCC TGTGCCCATT GCTCCAACTG CCCAACCTGA GGAACTGCTC
    AACGGAGCCC CCTCGCCACC AGCTGTGGAC TTAAGCCCAG TCAGTGAGCC AGAGGAGCAG
    GCCAAGGAGG TGACAGCATC AATGGCGCCC CCCACCATCC CCTCTGCTAC TCCAGCTACG
    GCTCCTTCAG CTACTTCCCC AGCTCAGGAG GAGGAAATGG AAGAAGAAGA AGAAGAGGAA
    GAAGGAGAAG CAGGAGAAGC AGGAGAAGCT GAGAGTGAGA AAGGAGGAGA GGAACTGCTC
    CCCCCAGAGA GTACCCCTAT TCCAGCCAAC TTGTCTCAGA ATTTGGAGGC AGCAGCAGCC
    ACTCAAGTGG CAGTATCTGT GCCAAAGAGG AGACGGAAAA TTAAGGAGCT AAATAAGAAG
    GAGGCTGTTG GAGACCTTCT GGATGCCTTC AAGGAGGCGA ACCCGGCAGT ACCAGAGGTG
    GAAAATCAGC CTCCTGCAGG CAGCAATCCA GGCCCAGAGT CTGAGGGCAG TGGTGTGCCC
    CCACGTCCTG AGGAAGCAGA TGAGACCTGG GACTCAAAGG AAGACAAAAT TCACAATGCT
    GAGAACATCC AGCCCGGGGA ACAGAAGTAT GAATATAAGT CAGATCAGTG GAAGCCTCTA
    AACCTAGAGG AGAAAAAACG TTACGACCGT GAGTTCCTGC TTGGTTTTCA GTTCATCTTT
    GCCAGTATGC AGAAGCCAGA GGGATTGCCA CATATCAGTG ACGTGGTGCT GGACAAGGCC
    AATAAAACAC CACTGCGGCC ACTGGATCCC ACTAGACTAC AAGGCATAAA TTGTGGCCCA
    GACTTCACTC CATCCTTTGC CAACCTTGGC CGGACAACCC TTAGCACCCG TGGGCCCCCA
    AGGGGTGGGC CAGGTGGGGA GCTGCCCCGT GGGCCGGCTG GCCTGGGACC CCGGCGCTCT
    CAGCAGGGAC CCCGAAAAGA ACCACGCAAG ATCATTGCCA CAGTGTTAAT GACCGAAGAT
    ATAAAACTGA ACAAAGCAGA GAAAGCCTGG AAACCCAGCA GCAAGCGGAC GGCGGCTGAT
    AAGGATCGAG GGGAAGAAGA TGCTGATGGC AGCAAAACCC AGGACCTATT CCGCAGGGTG
    CGCTCCATCC TGAATAAACT GACACCCCAG ATGTTCCAGC AGCTGATGAA GCAAGTGACG
    CAGCTGGCCA TCGACACCGA GGAACGCCTC AAAGGGGTCA TTGACCTCAT TTTTGAGAAG
    GCCATTTCAG AGCCCAACTT CTCTGTGGCC TATGCCAACA TGTGCCGCTG CCTCATGGCG
    CTGAAAGTGC CCACTACGGA AAAGCCAACA GTGACTGTGA ACTTCCGAAA GCTGTTGTTG
    AATCGATGTC AGAAGGAGTT TGAGAAAGAC AAAGATGATG ATGAGGTTTT TGAGAAGAAG
    CAAAAAGAGA TGGATGAAGC TGCTACGGCA GAGGAACGAG GACGCCTGAA GGAAGAGCTG
    GAAGAGGCTC GGGACATAGC CCGGCGGCGC TCTTTAGGGA ATATCAAGTT TATTGGAGAG
    TTGTTCAAAC TGAAGATGTT AACAGAGGCA ATAATGCATG ACTGTGTGGT CAAACTGCTT
    AAGAACCATG ATGAAGAGTC CCTTGAGTGC CTTTGTCGTC TGCTCACCAC CATTGGCAAA
    GACCTGGACT TTGAAAAAGC CAAGCCCCGA ATGGATCAGT ATTTCAACCA GATGGAAAAA
    ATCATTAAAG AAAAGAAGAC GTCATCCCGC ATCCGCTTTA TGCTGCAGGA CGTGCTGGAT
    CTGCGAGGGA GCAATTGGGT GCCACGCCGA GGGGATCAGG GTCCCAAGAC CATTGACCAG
    ATCCATAAGG AGGCTGAGAT GGAAGAACAT CGAGAGCACA TCAAAGTGCA GCAGCTCATG
    GCCAAGGGCA GTGACAAGCG TCGGGGCGGT CCTCCAGGCC CTCCCATCAG CCGTGGACTT
    CCCCTTGTGG ATGATGGTGG CTGGAACACA GTTCCCATCA GCAAAGGTAG CCGCCCCATT
    GACACCTCAC GACTCACCAA GATCACCAAG CCTGGCTCCA TCGATTCTAA CAACCAGCTC
    TTTGCACCTG GAGGGCGACT GAGCTGGGGC AAGGGCAGCA GCGGAGGCTC AGGAGCCAAG
    CCCTCAGACG CAGCATCAGA AGCTGCTCGC CCAGCTACTA GTACTTTGAA TCGCTTCTCA
    GCCCTTCAAC AAGCGGTACC CACAGAAAGC ACAGATAATA GACGTGTGGT GCAGAGGAGT
    AGCTTGAGCC GAGAACGAGG CGAGAAAGCT GGAGACCGAG GAGACCGCCT AGAGCGGAGT
    GAACGGGGAG GGGACCGTGG GGACCGGCTT GATCGTGCGC GGACACCTGC TACCAAGCGG
    AGCTTCAGCA AGGAAGTGGA GGAGCGGAGT AGAGAACGGC CCTCCCAGCC TGAGGGGCTG
    CGCAAGGCAG CTAGCCTCAC GGAGGATCGG GACCGTGGGC GGGATGCCGT GAAGCGAGAA
    GCTGCCCTAC CCCCAGTGAG CCCCCTGAAG GCGGCTCTCT CTGAGGAGGA GTTAGAGAAG
    AAATCCAAGG CTATCATTGA GGAATATCTC CATCTCAATG ACATGAAAGA GGCAGTCCAG
    TGCGTGCAGG AGCTGGCCTC ACCCTCCTTG CTCTTCATCT TTGTACGGCA TGGTGTCGAG
    TCTACGCTGG AGCGCAGTGC CATTGCTCGT GAGCATATGG GGCAGCTGCT GCACCAGCTG
    CTCTGTGCTG GGCATCTGTC TACTGCTCAG TACTACCAAG GGTTGTATGA AATCTTGGAA
    TTGGCTGAGG ACATGGAAAT TGACATCCCC CACGTGTGGC TCTACCTAGC GGAACTGGTA
    ACACCCATTC TGCAGGAAGG TGGGGTGCCC ATGGGGGAGC TGTTCAGGGA GATTACAAAG
    CCTCTGAGAC CGTTGGGCAA AGCTGCTTCC CTGTTGCTGG AGATCCTGGG CCTCCTGTGC
    AAAAGCATGG GTCCTAAAAA GGTGGGGACG CTGTGGCGAG AAGCCGGGCT TAGCTGGAAG
    GAATTTCTAC CTGAAGGCCA GGACATTGGT GCATTCGTCG CTGAACAGAA GGTGGAGTAT
    ACCCTGGGAG AGGAGTCGGA AGCCCCTGGC CAGAGGGCAC TCCCCTCCGA GGAGCTGAAC
    AGGCAGCTGG AGAAGCTGCT GAAGGAGGGC AGCAGTAACC AGCGGGTGTT CGACTGGATA
    GAGGCCAACC TGAGTGAGCA GCAGATAGTA TCCAACACGT TAGTTCGAGC CCTCATGACG
    GCTGTCTGCT ATTCTGCAAT TATTTTTGAG ACTCCCCTCC GAGTGGACGT TGCAGTGCTG
    AAAGCGCGAG CGAAGCTGCT GCAGAAATAC CTGTGTGACG AGCAGAAGGA GCTACAGGCG
    CTCTACGCCC TCCAGGCCCT TGTAGTGACC TTAGAACAGC CTCCCAACCT GCTGCGGATG
    TTCTTTGACG CACTGTATGA CGAGGACGTG GTGAAGGAGG ATGCCTTCTA CAGTTGGGAG
    AGTAGCAAGG ACCCCGCTGA GCAGCAGGGC AAGGGTGTGG CCCTTAAATC TGTCACAGCC
    TTCTTCAAGT GGCTCCGTGA AGCAGAGGAG GAGTCTGACC ACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_937885.1
    CDS349..4656
    Translation

    Target ORF information:

    RefSeq Version NM_198242.2
    Organism Homo sapiens(human)
    Definition Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_198242.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAACCAGC CACCCCAGAT TGCTCCCAAG AGGGAGCGTA AGACGATCCG AATTCGAGAT 
    CCAAACCAAG GAGGAAAGGA TATCACAGAG GAGATCATGT CTGGGGCCCG CACTGCCTCC
    ACACCCACCC CTCCCCAGAC GGGAGGCGGT CTGGAGCCTC AAGCTAATGG GGAGACGCCC
    CAGGTTGCTG TCATTGTCCG GCCAGATGAC CGGTCACAGG GAGCAATCAT TGCTGACCGG
    CCAGGGCTGC CTGGCCCAGA GCATAGCCCT TCAGAATCCC AGCCTTCGTC GCCTTCTCCG
    ACCCCATCAC CATCCCCAGT CTTGGAACCG GGGTCTGAGC CTAATCTCGC AGTCCTCTCT
    ATTCCTGGGG ACACTATGAC AACTATACAA ATGTCTGTAG AAGAATCAAC CCCCATCTCC
    CGTGAAACTG GGGAGCCATA TCGCCTCTCT CCAGAACCCA CTCCTCTCGC CGAACCCATA
    CTGGAAGTAG AAGTGACACT TAGCAAACCG GTTCCAGAAT CTGAGTTTTC TTCCAGTCCT
    CTCCAGGCTC CCACCCCTTT GGCATCTCAC ACAGTGGAAA TTCATGAGCC TAATGGCATG
    GTCCCATCTG AAGATCTGGA ACCAGAGGTG GAGTCAAGCC CAGAGCTTGC TCCTCCCCCA
    GCTTGCCCCT CCGAATCCCC TGTGCCCATT GCTCCAACTG CCCAACCTGA GGAACTGCTC
    AACGGAGCCC CCTCGCCACC AGCTGTGGAC TTAAGCCCAG TCAGTGAGCC AGAGGAGCAG
    GCCAAGGAGG TGACAGCATC AATGGCGCCC CCCACCATCC CCTCTGCTAC TCCAGCTACG
    GCTCCTTCAG CTACTTCCCC AGCTCAGGAG GAGGAAATGG AAGAAGAAGA AGAAGAGGAA
    GAAGGAGAAG CAGGAGAAGC AGGAGAAGCT GAGAGTGAGA AAGGAGGAGA GGAACTGCTC
    CCCCCAGAGA GTACCCCTAT TCCAGCCAAC TTGTCTCAGA ATTTGGAGGC AGCAGCAGCC
    ACTCAAGTGG CAGTATCTGT GCCAAAGAGG AGACGGAAAA TTAAGGAGCT AAATAAGAAG
    GAGGCTGTTG GAGACCTTCT GGATGCCTTC AAGGAGGCGA ACCCGGCAGT ACCAGAGGTG
    GAAAATCAGC CTCCTGCAGG CAGCAATCCA GGCCCAGAGT CTGAGGGCAG TGGTGTGCCC
    CCACGTCCTG AGGAAGCAGA TGAGACCTGG GACTCAAAGG AAGACAAAAT TCACAATGCT
    GAGAACATCC AGCCCGGGGA ACAGAAGTAT GAATATAAGT CAGATCAGTG GAAGCCTCTA
    AACCTAGAGG AGAAAAAACG TTACGACCGT GAGTTCCTGC TTGGTTTTCA GTTCATCTTT
    GCCAGTATGC AGAAGCCAGA GGGATTGCCA CATATCAGTG ACGTGGTGCT GGACAAGGCC
    AATAAAACAC CACTGCGGCC ACTGGATCCC ACTAGACTAC AAGGCATAAA TTGTGGCCCA
    GACTTCACTC CATCCTTTGC CAACCTTGGC CGGACAACCC TTAGCACCCG TGGGCCCCCA
    AGGGGTGGGC CAGGTGGGGA GCTGCCCCGT GGGCCGGCTG GCCTGGGACC CCGGCGCTCT
    CAGCAGGGAC CCCGAAAAGA ACCACGCAAG ATCATTGCCA CAGTGTTAAT GACCGAAGAT
    ATAAAACTGA ACAAAGCAGA GAAAGCCTGG AAACCCAGCA GCAAGCGGAC GGCGGCTGAT
    AAGGATCGAG GGGAAGAAGA TGCTGATGGC AGCAAAACCC AGGACCTATT CCGCAGGGTG
    CGCTCCATCC TGAATAAACT GACACCCCAG ATGTTCCAGC AGCTGATGAA GCAAGTGACG
    CAGCTGGCCA TCGACACCGA GGAACGCCTC AAAGGGGTCA TTGACCTCAT TTTTGAGAAG
    GCCATTTCAG AGCCCAACTT CTCTGTGGCC TATGCCAACA TGTGCCGCTG CCTCATGGCG
    CTGAAAGTGC CCACTACGGA AAAGCCAACA GTGACTGTGA ACTTCCGAAA GCTGTTGTTG
    AATCGATGTC AGAAGGAGTT TGAGAAAGAC AAAGATGATG ATGAGGTTTT TGAGAAGAAG
    CAAAAAGAGA TGGATGAAGC TGCTACGGCA GAGGAACGAG GACGCCTGAA GGAAGAGCTG
    GAAGAGGCTC GGGACATAGC CCGGCGGCGC TCTTTAGGGA ATATCAAGTT TATTGGAGAG
    TTGTTCAAAC TGAAGATGTT AACAGAGGCA ATAATGCATG ACTGTGTGGT CAAACTGCTT
    AAGAACCATG ATGAAGAGTC CCTTGAGTGC CTTTGTCGTC TGCTCACCAC CATTGGCAAA
    GACCTGGACT TTGAAAAAGC CAAGCCCCGA ATGGATCAGT ATTTCAACCA GATGGAAAAA
    ATCATTAAAG AAAAGAAGAC GTCATCCCGC ATCCGCTTTA TGCTGCAGGA CGTGCTGGAT
    CTGCGAGGGA GCAATTGGGT GCCACGCCGA GGGGATCAGG GTCCCAAGAC CATTGACCAG
    ATCCATAAGG AGGCTGAGAT GGAAGAACAT CGAGAGCACA TCAAAGTGCA GCAGCTCATG
    GCCAAGGGCA GTGACAAGCG TCGGGGCGGT CCTCCAGGCC CTCCCATCAG CCGTGGACTT
    CCCCTTGTGG ATGATGGTGG CTGGAACACA GTTCCCATCA GCAAAGGTAG CCGCCCCATT
    GACACCTCAC GACTCACCAA GATCACCAAG CCTGGCTCCA TCGATTCTAA CAACCAGCTC
    TTTGCACCTG GAGGGCGACT GAGCTGGGGC AAGGGCAGCA GCGGAGGCTC AGGAGCCAAG
    CCCTCAGACG CAGCATCAGA AGCTGCTCGC CCAGCTACTA GTACTTTGAA TCGCTTCTCA
    GCCCTTCAAC AAGCGGTACC CACAGAAAGC ACAGATAATA GACGTGTGGT GCAGAGGAGT
    AGCTTGAGCC GAGAACGAGG CGAGAAAGCT GGAGACCGAG GAGACCGCCT AGAGCGGAGT
    GAACGGGGAG GGGACCGTGG GGACCGGCTT GATCGTGCGC GGACACCTGC TACCAAGCGG
    AGCTTCAGCA AGGAAGTGGA GGAGCGGAGT AGAGAACGGC CCTCCCAGCC TGAGGGGCTG
    CGCAAGGCAG CTAGCCTCAC GGAGGATCGG GACCGTGGGC GGGATGCCGT GAAGCGAGAA
    GCTGCCCTAC CCCCAGTGAG CCCCCTGAAG GCGGCTCTCT CTGAGGAGGA GTTAGAGAAG
    AAATCCAAGG CTATCATTGA GGAATATCTC CATCTCAATG ACATGAAAGA GGCAGTCCAG
    TGCGTGCAGG AGCTGGCCTC ACCCTCCTTG CTCTTCATCT TTGTACGGCA TGGTGTCGAG
    TCTACGCTGG AGCGCAGTGC CATTGCTCGT GAGCATATGG GGCAGCTGCT GCACCAGCTG
    CTCTGTGCTG GGCATCTGTC TACTGCTCAG TACTACCAAG GGTTGTATGA AATCTTGGAA
    TTGGCTGAGG ACATGGAAAT TGACATCCCC CACGTGTGGC TCTACCTAGC GGAACTGGTA
    ACACCCATTC TGCAGGAAGG TGGGGTGCCC ATGGGGGAGC TGTTCAGGGA GATTACAAAG
    CCTCTGAGAC CGTTGGGCAA AGCTGCTTCC CTGTTGCTGG AGATCCTGGG CCTCCTGTGC
    AAAAGCATGG GTCCTAAAAA GGTGGGGACG CTGTGGCGAG AAGCCGGGCT TAGCTGGAAG
    GAATTTCTAC CTGAAGGCCA GGACATTGGT GCATTCGTCG CTGAACAGAA GGTGGAGTAT
    ACCCTGGGAG AGGAGTCGGA AGCCCCTGGC CAGAGGGCAC TCCCCTCCGA GGAGCTGAAC
    AGGCAGCTGG AGAAGCTGCT GAAGGAGGGC AGCAGTAACC AGCGGGTGTT CGACTGGATA
    GAGGCCAACC TGAGTGAGCA GCAGATAGTA TCCAACACGT TAGTTCGAGC CCTCATGACG
    GCTGTCTGCT ATTCTGCAAT TATTTTTGAG ACTCCCCTCC GAGTGGACGT TGCAGTGCTG
    AAAGCGCGAG CGAAGCTGCT GCAGAAATAC CTGTGTGACG AGCAGAAGGA GCTACAGGCG
    CTCTACGCCC TCCAGGCCCT TGTAGTGACC TTAGAACAGC CTCCCAACCT GCTGCGGATG
    TTCTTTGACG CACTGTATGA CGAGGACGTG GTGAAGGAGG ATGCCTTCTA CAGTTGGGAG
    AGTAGCAAGG ACCCCGCTGA GCAGCAGGGC AAGGGTGTGG CCCTTAAATC TGTCACAGCC
    TTCTTCAAGT GGCTCCGTGA AGCAGAGGAG GAGTCTGACC ACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13773
    Clone ID Related Accession (Same CDS sequence) NM_198242.2 , NM_198242.3
    Accession Version NM_198242.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-29
    Organism Homo sapiens(human)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20. On Jun 2, 2019 this sequence version replaced NM_198242.2. Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, uses a downstream start codon, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (3) is shorter at the N-terminus and lacks an internal residue, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL832754.1, SRR1803617.86057.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAACCAGC CACCCCAGAT TGCTCCCAAG AGGGAGCGTA AGACGATCCG AATTCGAGAT 
    CCAAACCAAG GAGGAAAGGA TATCACAGAG GAGATCATGT CTGGGGCCCG CACTGCCTCC
    ACACCCACCC CTCCCCAGAC GGGAGGCGGT CTGGAGCCTC AAGCTAATGG GGAGACGCCC
    CAGGTTGCTG TCATTGTCCG GCCAGATGAC CGGTCACAGG GAGCAATCAT TGCTGACCGG
    CCAGGGCTGC CTGGCCCAGA GCATAGCCCT TCAGAATCCC AGCCTTCGTC GCCTTCTCCG
    ACCCCATCAC CATCCCCAGT CTTGGAACCG GGGTCTGAGC CTAATCTCGC AGTCCTCTCT
    ATTCCTGGGG ACACTATGAC AACTATACAA ATGTCTGTAG AAGAATCAAC CCCCATCTCC
    CGTGAAACTG GGGAGCCATA TCGCCTCTCT CCAGAACCCA CTCCTCTCGC CGAACCCATA
    CTGGAAGTAG AAGTGACACT TAGCAAACCG GTTCCAGAAT CTGAGTTTTC TTCCAGTCCT
    CTCCAGGCTC CCACCCCTTT GGCATCTCAC ACAGTGGAAA TTCATGAGCC TAATGGCATG
    GTCCCATCTG AAGATCTGGA ACCAGAGGTG GAGTCAAGCC CAGAGCTTGC TCCTCCCCCA
    GCTTGCCCCT CCGAATCCCC TGTGCCCATT GCTCCAACTG CCCAACCTGA GGAACTGCTC
    AACGGAGCCC CCTCGCCACC AGCTGTGGAC TTAAGCCCAG TCAGTGAGCC AGAGGAGCAG
    GCCAAGGAGG TGACAGCATC AATGGCGCCC CCCACCATCC CCTCTGCTAC TCCAGCTACG
    GCTCCTTCAG CTACTTCCCC AGCTCAGGAG GAGGAAATGG AAGAAGAAGA AGAAGAGGAA
    GAAGGAGAAG CAGGAGAAGC AGGAGAAGCT GAGAGTGAGA AAGGAGGAGA GGAACTGCTC
    CCCCCAGAGA GTACCCCTAT TCCAGCCAAC TTGTCTCAGA ATTTGGAGGC AGCAGCAGCC
    ACTCAAGTGG CAGTATCTGT GCCAAAGAGG AGACGGAAAA TTAAGGAGCT AAATAAGAAG
    GAGGCTGTTG GAGACCTTCT GGATGCCTTC AAGGAGGCGA ACCCGGCAGT ACCAGAGGTG
    GAAAATCAGC CTCCTGCAGG CAGCAATCCA GGCCCAGAGT CTGAGGGCAG TGGTGTGCCC
    CCACGTCCTG AGGAAGCAGA TGAGACCTGG GACTCAAAGG AAGACAAAAT TCACAATGCT
    GAGAACATCC AGCCCGGGGA ACAGAAGTAT GAATATAAGT CAGATCAGTG GAAGCCTCTA
    AACCTAGAGG AGAAAAAACG TTACGACCGT GAGTTCCTGC TTGGTTTTCA GTTCATCTTT
    GCCAGTATGC AGAAGCCAGA GGGATTGCCA CATATCAGTG ACGTGGTGCT GGACAAGGCC
    AATAAAACAC CACTGCGGCC ACTGGATCCC ACTAGACTAC AAGGCATAAA TTGTGGCCCA
    GACTTCACTC CATCCTTTGC CAACCTTGGC CGGACAACCC TTAGCACCCG TGGGCCCCCA
    AGGGGTGGGC CAGGTGGGGA GCTGCCCCGT GGGCCGGCTG GCCTGGGACC CCGGCGCTCT
    CAGCAGGGAC CCCGAAAAGA ACCACGCAAG ATCATTGCCA CAGTGTTAAT GACCGAAGAT
    ATAAAACTGA ACAAAGCAGA GAAAGCCTGG AAACCCAGCA GCAAGCGGAC GGCGGCTGAT
    AAGGATCGAG GGGAAGAAGA TGCTGATGGC AGCAAAACCC AGGACCTATT CCGCAGGGTG
    CGCTCCATCC TGAATAAACT GACACCCCAG ATGTTCCAGC AGCTGATGAA GCAAGTGACG
    CAGCTGGCCA TCGACACCGA GGAACGCCTC AAAGGGGTCA TTGACCTCAT TTTTGAGAAG
    GCCATTTCAG AGCCCAACTT CTCTGTGGCC TATGCCAACA TGTGCCGCTG CCTCATGGCG
    CTGAAAGTGC CCACTACGGA AAAGCCAACA GTGACTGTGA ACTTCCGAAA GCTGTTGTTG
    AATCGATGTC AGAAGGAGTT TGAGAAAGAC AAAGATGATG ATGAGGTTTT TGAGAAGAAG
    CAAAAAGAGA TGGATGAAGC TGCTACGGCA GAGGAACGAG GACGCCTGAA GGAAGAGCTG
    GAAGAGGCTC GGGACATAGC CCGGCGGCGC TCTTTAGGGA ATATCAAGTT TATTGGAGAG
    TTGTTCAAAC TGAAGATGTT AACAGAGGCA ATAATGCATG ACTGTGTGGT CAAACTGCTT
    AAGAACCATG ATGAAGAGTC CCTTGAGTGC CTTTGTCGTC TGCTCACCAC CATTGGCAAA
    GACCTGGACT TTGAAAAAGC CAAGCCCCGA ATGGATCAGT ATTTCAACCA GATGGAAAAA
    ATCATTAAAG AAAAGAAGAC GTCATCCCGC ATCCGCTTTA TGCTGCAGGA CGTGCTGGAT
    CTGCGAGGGA GCAATTGGGT GCCACGCCGA GGGGATCAGG GTCCCAAGAC CATTGACCAG
    ATCCATAAGG AGGCTGAGAT GGAAGAACAT CGAGAGCACA TCAAAGTGCA GCAGCTCATG
    GCCAAGGGCA GTGACAAGCG TCGGGGCGGT CCTCCAGGCC CTCCCATCAG CCGTGGACTT
    CCCCTTGTGG ATGATGGTGG CTGGAACACA GTTCCCATCA GCAAAGGTAG CCGCCCCATT
    GACACCTCAC GACTCACCAA GATCACCAAG CCTGGCTCCA TCGATTCTAA CAACCAGCTC
    TTTGCACCTG GAGGGCGACT GAGCTGGGGC AAGGGCAGCA GCGGAGGCTC AGGAGCCAAG
    CCCTCAGACG CAGCATCAGA AGCTGCTCGC CCAGCTACTA GTACTTTGAA TCGCTTCTCA
    GCCCTTCAAC AAGCGGTACC CACAGAAAGC ACAGATAATA GACGTGTGGT GCAGAGGAGT
    AGCTTGAGCC GAGAACGAGG CGAGAAAGCT GGAGACCGAG GAGACCGCCT AGAGCGGAGT
    GAACGGGGAG GGGACCGTGG GGACCGGCTT GATCGTGCGC GGACACCTGC TACCAAGCGG
    AGCTTCAGCA AGGAAGTGGA GGAGCGGAGT AGAGAACGGC CCTCCCAGCC TGAGGGGCTG
    CGCAAGGCAG CTAGCCTCAC GGAGGATCGG GACCGTGGGC GGGATGCCGT GAAGCGAGAA
    GCTGCCCTAC CCCCAGTGAG CCCCCTGAAG GCGGCTCTCT CTGAGGAGGA GTTAGAGAAG
    AAATCCAAGG CTATCATTGA GGAATATCTC CATCTCAATG ACATGAAAGA GGCAGTCCAG
    TGCGTGCAGG AGCTGGCCTC ACCCTCCTTG CTCTTCATCT TTGTACGGCA TGGTGTCGAG
    TCTACGCTGG AGCGCAGTGC CATTGCTCGT GAGCATATGG GGCAGCTGCT GCACCAGCTG
    CTCTGTGCTG GGCATCTGTC TACTGCTCAG TACTACCAAG GGTTGTATGA AATCTTGGAA
    TTGGCTGAGG ACATGGAAAT TGACATCCCC CACGTGTGGC TCTACCTAGC GGAACTGGTA
    ACACCCATTC TGCAGGAAGG TGGGGTGCCC ATGGGGGAGC TGTTCAGGGA GATTACAAAG
    CCTCTGAGAC CGTTGGGCAA AGCTGCTTCC CTGTTGCTGG AGATCCTGGG CCTCCTGTGC
    AAAAGCATGG GTCCTAAAAA GGTGGGGACG CTGTGGCGAG AAGCCGGGCT TAGCTGGAAG
    GAATTTCTAC CTGAAGGCCA GGACATTGGT GCATTCGTCG CTGAACAGAA GGTGGAGTAT
    ACCCTGGGAG AGGAGTCGGA AGCCCCTGGC CAGAGGGCAC TCCCCTCCGA GGAGCTGAAC
    AGGCAGCTGG AGAAGCTGCT GAAGGAGGGC AGCAGTAACC AGCGGGTGTT CGACTGGATA
    GAGGCCAACC TGAGTGAGCA GCAGATAGTA TCCAACACGT TAGTTCGAGC CCTCATGACG
    GCTGTCTGCT ATTCTGCAAT TATTTTTGAG ACTCCCCTCC GAGTGGACGT TGCAGTGCTG
    AAAGCGCGAG CGAAGCTGCT GCAGAAATAC CTGTGTGACG AGCAGAAGGA GCTACAGGCG
    CTCTACGCCC TCCAGGCCCT TGTAGTGACC TTAGAACAGC CTCCCAACCT GCTGCGGATG
    TTCTTTGACG CACTGTATGA CGAGGACGTG GTGAAGGAGG ATGCCTTCTA CAGTTGGGAG
    AGTAGCAAGG ACCCCGCTGA GCAGCAGGGC AAGGGTGTGG CCCTTAAATC TGTCACAGCC
    TTCTTCAAGT GGCTCCGTGA AGCAGAGGAG GAGTCTGACC ACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_937885.1
    CDS238..4545
    Translation

    Target ORF information:

    RefSeq Version NM_198242.3
    Organism Homo sapiens(human)
    Definition Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_198242.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAACCAGC CACCCCAGAT TGCTCCCAAG AGGGAGCGTA AGACGATCCG AATTCGAGAT 
    CCAAACCAAG GAGGAAAGGA TATCACAGAG GAGATCATGT CTGGGGCCCG CACTGCCTCC
    ACACCCACCC CTCCCCAGAC GGGAGGCGGT CTGGAGCCTC AAGCTAATGG GGAGACGCCC
    CAGGTTGCTG TCATTGTCCG GCCAGATGAC CGGTCACAGG GAGCAATCAT TGCTGACCGG
    CCAGGGCTGC CTGGCCCAGA GCATAGCCCT TCAGAATCCC AGCCTTCGTC GCCTTCTCCG
    ACCCCATCAC CATCCCCAGT CTTGGAACCG GGGTCTGAGC CTAATCTCGC AGTCCTCTCT
    ATTCCTGGGG ACACTATGAC AACTATACAA ATGTCTGTAG AAGAATCAAC CCCCATCTCC
    CGTGAAACTG GGGAGCCATA TCGCCTCTCT CCAGAACCCA CTCCTCTCGC CGAACCCATA
    CTGGAAGTAG AAGTGACACT TAGCAAACCG GTTCCAGAAT CTGAGTTTTC TTCCAGTCCT
    CTCCAGGCTC CCACCCCTTT GGCATCTCAC ACAGTGGAAA TTCATGAGCC TAATGGCATG
    GTCCCATCTG AAGATCTGGA ACCAGAGGTG GAGTCAAGCC CAGAGCTTGC TCCTCCCCCA
    GCTTGCCCCT CCGAATCCCC TGTGCCCATT GCTCCAACTG CCCAACCTGA GGAACTGCTC
    AACGGAGCCC CCTCGCCACC AGCTGTGGAC TTAAGCCCAG TCAGTGAGCC AGAGGAGCAG
    GCCAAGGAGG TGACAGCATC AATGGCGCCC CCCACCATCC CCTCTGCTAC TCCAGCTACG
    GCTCCTTCAG CTACTTCCCC AGCTCAGGAG GAGGAAATGG AAGAAGAAGA AGAAGAGGAA
    GAAGGAGAAG CAGGAGAAGC AGGAGAAGCT GAGAGTGAGA AAGGAGGAGA GGAACTGCTC
    CCCCCAGAGA GTACCCCTAT TCCAGCCAAC TTGTCTCAGA ATTTGGAGGC AGCAGCAGCC
    ACTCAAGTGG CAGTATCTGT GCCAAAGAGG AGACGGAAAA TTAAGGAGCT AAATAAGAAG
    GAGGCTGTTG GAGACCTTCT GGATGCCTTC AAGGAGGCGA ACCCGGCAGT ACCAGAGGTG
    GAAAATCAGC CTCCTGCAGG CAGCAATCCA GGCCCAGAGT CTGAGGGCAG TGGTGTGCCC
    CCACGTCCTG AGGAAGCAGA TGAGACCTGG GACTCAAAGG AAGACAAAAT TCACAATGCT
    GAGAACATCC AGCCCGGGGA ACAGAAGTAT GAATATAAGT CAGATCAGTG GAAGCCTCTA
    AACCTAGAGG AGAAAAAACG TTACGACCGT GAGTTCCTGC TTGGTTTTCA GTTCATCTTT
    GCCAGTATGC AGAAGCCAGA GGGATTGCCA CATATCAGTG ACGTGGTGCT GGACAAGGCC
    AATAAAACAC CACTGCGGCC ACTGGATCCC ACTAGACTAC AAGGCATAAA TTGTGGCCCA
    GACTTCACTC CATCCTTTGC CAACCTTGGC CGGACAACCC TTAGCACCCG TGGGCCCCCA
    AGGGGTGGGC CAGGTGGGGA GCTGCCCCGT GGGCCGGCTG GCCTGGGACC CCGGCGCTCT
    CAGCAGGGAC CCCGAAAAGA ACCACGCAAG ATCATTGCCA CAGTGTTAAT GACCGAAGAT
    ATAAAACTGA ACAAAGCAGA GAAAGCCTGG AAACCCAGCA GCAAGCGGAC GGCGGCTGAT
    AAGGATCGAG GGGAAGAAGA TGCTGATGGC AGCAAAACCC AGGACCTATT CCGCAGGGTG
    CGCTCCATCC TGAATAAACT GACACCCCAG ATGTTCCAGC AGCTGATGAA GCAAGTGACG
    CAGCTGGCCA TCGACACCGA GGAACGCCTC AAAGGGGTCA TTGACCTCAT TTTTGAGAAG
    GCCATTTCAG AGCCCAACTT CTCTGTGGCC TATGCCAACA TGTGCCGCTG CCTCATGGCG
    CTGAAAGTGC CCACTACGGA AAAGCCAACA GTGACTGTGA ACTTCCGAAA GCTGTTGTTG
    AATCGATGTC AGAAGGAGTT TGAGAAAGAC AAAGATGATG ATGAGGTTTT TGAGAAGAAG
    CAAAAAGAGA TGGATGAAGC TGCTACGGCA GAGGAACGAG GACGCCTGAA GGAAGAGCTG
    GAAGAGGCTC GGGACATAGC CCGGCGGCGC TCTTTAGGGA ATATCAAGTT TATTGGAGAG
    TTGTTCAAAC TGAAGATGTT AACAGAGGCA ATAATGCATG ACTGTGTGGT CAAACTGCTT
    AAGAACCATG ATGAAGAGTC CCTTGAGTGC CTTTGTCGTC TGCTCACCAC CATTGGCAAA
    GACCTGGACT TTGAAAAAGC CAAGCCCCGA ATGGATCAGT ATTTCAACCA GATGGAAAAA
    ATCATTAAAG AAAAGAAGAC GTCATCCCGC ATCCGCTTTA TGCTGCAGGA CGTGCTGGAT
    CTGCGAGGGA GCAATTGGGT GCCACGCCGA GGGGATCAGG GTCCCAAGAC CATTGACCAG
    ATCCATAAGG AGGCTGAGAT GGAAGAACAT CGAGAGCACA TCAAAGTGCA GCAGCTCATG
    GCCAAGGGCA GTGACAAGCG TCGGGGCGGT CCTCCAGGCC CTCCCATCAG CCGTGGACTT
    CCCCTTGTGG ATGATGGTGG CTGGAACACA GTTCCCATCA GCAAAGGTAG CCGCCCCATT
    GACACCTCAC GACTCACCAA GATCACCAAG CCTGGCTCCA TCGATTCTAA CAACCAGCTC
    TTTGCACCTG GAGGGCGACT GAGCTGGGGC AAGGGCAGCA GCGGAGGCTC AGGAGCCAAG
    CCCTCAGACG CAGCATCAGA AGCTGCTCGC CCAGCTACTA GTACTTTGAA TCGCTTCTCA
    GCCCTTCAAC AAGCGGTACC CACAGAAAGC ACAGATAATA GACGTGTGGT GCAGAGGAGT
    AGCTTGAGCC GAGAACGAGG CGAGAAAGCT GGAGACCGAG GAGACCGCCT AGAGCGGAGT
    GAACGGGGAG GGGACCGTGG GGACCGGCTT GATCGTGCGC GGACACCTGC TACCAAGCGG
    AGCTTCAGCA AGGAAGTGGA GGAGCGGAGT AGAGAACGGC CCTCCCAGCC TGAGGGGCTG
    CGCAAGGCAG CTAGCCTCAC GGAGGATCGG GACCGTGGGC GGGATGCCGT GAAGCGAGAA
    GCTGCCCTAC CCCCAGTGAG CCCCCTGAAG GCGGCTCTCT CTGAGGAGGA GTTAGAGAAG
    AAATCCAAGG CTATCATTGA GGAATATCTC CATCTCAATG ACATGAAAGA GGCAGTCCAG
    TGCGTGCAGG AGCTGGCCTC ACCCTCCTTG CTCTTCATCT TTGTACGGCA TGGTGTCGAG
    TCTACGCTGG AGCGCAGTGC CATTGCTCGT GAGCATATGG GGCAGCTGCT GCACCAGCTG
    CTCTGTGCTG GGCATCTGTC TACTGCTCAG TACTACCAAG GGTTGTATGA AATCTTGGAA
    TTGGCTGAGG ACATGGAAAT TGACATCCCC CACGTGTGGC TCTACCTAGC GGAACTGGTA
    ACACCCATTC TGCAGGAAGG TGGGGTGCCC ATGGGGGAGC TGTTCAGGGA GATTACAAAG
    CCTCTGAGAC CGTTGGGCAA AGCTGCTTCC CTGTTGCTGG AGATCCTGGG CCTCCTGTGC
    AAAAGCATGG GTCCTAAAAA GGTGGGGACG CTGTGGCGAG AAGCCGGGCT TAGCTGGAAG
    GAATTTCTAC CTGAAGGCCA GGACATTGGT GCATTCGTCG CTGAACAGAA GGTGGAGTAT
    ACCCTGGGAG AGGAGTCGGA AGCCCCTGGC CAGAGGGCAC TCCCCTCCGA GGAGCTGAAC
    AGGCAGCTGG AGAAGCTGCT GAAGGAGGGC AGCAGTAACC AGCGGGTGTT CGACTGGATA
    GAGGCCAACC TGAGTGAGCA GCAGATAGTA TCCAACACGT TAGTTCGAGC CCTCATGACG
    GCTGTCTGCT ATTCTGCAAT TATTTTTGAG ACTCCCCTCC GAGTGGACGT TGCAGTGCTG
    AAAGCGCGAG CGAAGCTGCT GCAGAAATAC CTGTGTGACG AGCAGAAGGA GCTACAGGCG
    CTCTACGCCC TCCAGGCCCT TGTAGTGACC TTAGAACAGC CTCCCAACCT GCTGCGGATG
    TTCTTTGACG CACTGTATGA CGAGGACGTG GTGAAGGAGG ATGCCTTCTA CAGTTGGGAG
    AGTAGCAAGG ACCCCGCTGA GCAGCAGGGC AAGGGTGTGG CCCTTAAATC TGTCACAGCC
    TTCTTCAAGT GGCTCCGTGA AGCAGAGGAG GAGTCTGACC ACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13775
    Clone ID Related Accession (Same CDS sequence) NM_001194946.1 , NM_001194947.1
    Accession Version NM_001194946.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4821bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform 6
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR980540.1, BC140892.1, BC140896.1 and BQ011010.1. Transcript Variant: This variant (6) differs in the 5' UTR, includes an additional in-frame exon in the 5' coding region, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (6) is longer than isoform 1. Both variants 6 and 7 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC140892.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGTC CACAGGCCCC CCACCCGCCC CATCCCCCGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCGCCG GTGGTGTTCA GTACGCCACA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC
    CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGGCG TTCCACATAC
    GTTGTCCCGA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA
    AGCCCTACAG AATTTGGGAC CTACGCTGGC GCCTACTATC CAGCCCAAGG GGTGCAGCAG
    TTTCCCACTG GCGTGGCCCC CGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCC
    AAGAGGGAGC GTAAGACGAT CCGAATTCGA GATCCAAACC AAGGAGGAAA GGATATCACA
    GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CCCCTCCCCA GACGGGAGGC
    GGTCTGGAGC CTCAAGCTAA TGGGGAGACG CCCCAGGTTG CTGTCATTGT CCGGCCAGAT
    GACCGGTCAC AGGGAGCAAT CATTGCTGAC CGGCCAGGGC TGCCTGGCCC AGAGCATAGC
    CCTTCAGAAT CCCAGCCTTC GTCGCCTTCT CCGACCCCAT CACCATCCCC AGTCTTGGAA
    CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTATTCCTG GGGACACTAT GACAACTATA
    CAAATGTCTG TAGAAGAATC AACCCCCATC TCCCGTGAAA CTGGGGAGCC ATATCGCCTC
    TCTCCAGAAC CCACTCCTCT CGCCGAACCC ATACTGGAAG TAGAAGTGAC ACTTAGCAAA
    CCGGTTCCAG AATCTGAGTT TTCTTCCAGT CCTCTCCAGG CTCCCACCCC TTTGGCATCT
    CACACAGTGG AAATTCATGA GCCTAATGGC ATGGTCCCAT CTGAAGATCT GGAACCAGAG
    GTGGAGTCAA GCCCAGAGCT TGCTCCTCCC CCAGCTTGCC CCTCCGAATC CCCTGTGCCC
    ATTGCTCCAA CTGCCCAACC TGAGGAACTG CTCAACGGAG CCCCCTCGCC ACCAGCTGTG
    GACTTAAGCC CAGTCAGTGA GCCAGAGGAG CAGGCCAAGG AGGTGACAGC ATCAATGGCG
    CCCCCCACCA TCCCCTCTGC TACTCCAGCT ACGGCTCCTT CAGCTACTTC CCCAGCTCAG
    GAGGAGGAAA TGGAAGAAGA AGAAGAAGAG GAAGAAGGAG AAGCAGGAGA AGCAGGAGAA
    GCTGAGAGTG AGAAAGGAGG AGAGGAACTG CTCCCCCCAG AGAGTACCCC TATTCCAGCC
    AACTTGTCTC AGAATTTGGA GGCAGCAGCA GCCACTCAAG TGGCAGTATC TGTGCCAAAG
    AGGAGACGGA AAATTAAGGA GCTAAATAAG AAGGAGGCTG TTGGAGACCT TCTGGATGCC
    TTCAAGGAGG CGAACCCGGC AGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCAGCAAT
    CCAGGCCCAG AGTCTGAGGG CAGTGGTGTG CCCCCACGTC CTGAGGAAGC AGATGAGACC
    TGGGACTCAA AGGAAGACAA AATTCACAAT GCTGAGAACA TCCAGCCCGG GGAACAGAAG
    TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTAG AGGAGAAAAA ACGTTACGAC
    CGTGAGTTCC TGCTTGGTTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC AGAGGGATTG
    CCACATATCA GTGACGTGGT GCTGGACAAG GCCAATAAAA CACCACTGCG GCCACTGGAT
    CCCACTAGAC TACAAGGCAT AAATTGTGGC CCAGACTTCA CTCCATCCTT TGCCAACCTT
    GGCCGGACAA CCCTTAGCAC CCGTGGGCCC CCAAGGGGTG GGCCAGGTGG GGAGCTGCCC
    CGTGGGCCGG CTGGCCTGGG ACCCCGGCGC TCTCAGCAGG GACCCCGAAA AGAACCACGC
    AAGATCATTG CCACAGTGTT AATGACCGAA GATATAAAAC TGAACAAAGC AGAGAAAGCC
    TGGAAACCCA GCAGCAAGCG GACGGCGGCT GATAAGGATC GAGGGGAAGA AGATGCTGAT
    GGCAGCAAAA CCCAGGACCT ATTCCGCAGG GTGCGCTCCA TCCTGAATAA ACTGACACCC
    CAGATGTTCC AGCAGCTGAT GAAGCAAGTG ACGCAGCTGG CCATCGACAC CGAGGAACGC
    CTCAAAGGGG TCATTGACCT CATTTTTGAG AAGGCCATTT CAGAGCCCAA CTTCTCTGTG
    GCCTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC GGAAAAGCCA
    ACAGTGACTG TGAACTTCCG AAAGCTGTTG TTGAATCGAT GTCAGAAGGA GTTTGAGAAA
    GACAAAGATG ATGATGAGGT TTTTGAGAAG AAGCAAAAAG AGATGGATGA AGCTGCTACG
    GCAGAGGAAC GAGGACGCCT GAAGGAAGAG CTGGAAGAGG CTCGGGACAT AGCCCGGCGG
    CGCTCTTTAG GGAATATCAA GTTTATTGGA GAGTTGTTCA AACTGAAGAT GTTAACAGAG
    GCAATAATGC ATGACTGTGT GGTCAAACTG CTTAAGAACC ATGATGAAGA GTCCCTTGAG
    TGCCTTTGTC GTCTGCTCAC CACCATTGGC AAAGACCTGG ACTTTGAAAA AGCCAAGCCC
    CGAATGGATC AGTATTTCAA CCAGATGGAA AAAATCATTA AAGAAAAGAA GACGTCATCC
    CGCATCCGCT TTATGCTGCA GGACGTGCTG GATCTGCGAG GGAGCAATTG GGTGCCACGC
    CGAGGGGATC AGGGTCCCAA GACCATTGAC CAGATCCATA AGGAGGCTGA GATGGAAGAA
    CATCGAGAGC ACATCAAAGT GCAGCAGCTC ATGGCCAAGG GCAGTGACAA GCGTCGGGGC
    GGTCCTCCAG GCCCTCCCAT CAGCCGTGGA CTTCCCCTTG TGGATGATGG TGGCTGGAAC
    ACAGTTCCCA TCAGCAAAGG TAGCCGCCCC ATTGACACCT CACGACTCAC CAAGATCACC
    AAGCCTGGCT CCATCGATTC TAACAACCAG CTCTTTGCAC CTGGAGGGCG ACTGAGCTGG
    GGCAAGGGCA GCAGCGGAGG CTCAGGAGCC AAGCCCTCAG ACGCAGCATC AGAAGCTGCT
    CGCCCAGCTA CTAGTACTTT GAATCGCTTC TCAGCCCTTC AACAAGCGGT ACCCACAGAA
    AGCACAGATA ATAGACGTGT GGTGCAGAGG AGTAGCTTGA GCCGAGAACG AGGCGAGAAA
    GCTGGAGACC GAGGAGACCG CCTAGAGCGG AGTGAACGGG GAGGGGACCG TGGGGACCGG
    CTTGATCGTG CGCGGACACC TGCTACCAAG CGGAGCTTCA GCAAGGAAGT GGAGGAGCGG
    AGTAGAGAAC GGCCCTCCCA GCCTGAGGGG CTGCGCAAGG CAGCTAGCCT CACGGAGGAT
    CGGGACCGTG GGCGGGATGC CGTGAAGCGA GAAGCTGCCC TACCCCCAGT GAGCCCCCTG
    AAGGCGGCTC TCTCTGAGGA GGAGTTAGAG AAGAAATCCA AGGCTATCAT TGAGGAATAT
    CTCCATCTCA ATGACATGAA AGAGGCAGTC CAGTGCGTGC AGGAGCTGGC CTCACCCTCC
    TTGCTCTTCA TCTTTGTACG GCATGGTGTC GAGTCTACGC TGGAGCGCAG TGCCATTGCT
    CGTGAGCATA TGGGGCAGCT GCTGCACCAG CTGCTCTGTG CTGGGCATCT GTCTACTGCT
    CAGTACTACC AAGGGTTGTA TGAAATCTTG GAATTGGCTG AGGACATGGA AATTGACATC
    CCCCACGTGT GGCTCTACCT AGCGGAACTG GTAACACCCA TTCTGCAGGA AGGTGGGGTG
    CCCATGGGGG AGCTGTTCAG GGAGATTACA AAGCCTCTGA GACCGTTGGG CAAAGCTGCT
    TCCCTGTTGC TGGAGATCCT GGGCCTCCTG TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG
    ACGCTGTGGC GAGAAGCCGG GCTTAGCTGG AAGGAATTTC TACCTGAAGG CCAGGACATT
    GGTGCATTCG TCGCTGAACA GAAGGTGGAG TATACCCTGG GAGAGGAGTC GGAAGCCCCT
    GGCCAGAGGG CACTCCCCTC CGAGGAGCTG AACAGGCAGC TGGAGAAGCT GCTGAAGGAG
    GGCAGCAGTA ACCAGCGGGT GTTCGACTGG ATAGAGGCCA ACCTGAGTGA GCAGCAGATA
    GTATCCAACA CGTTAGTTCG AGCCCTCATG ACGGCTGTCT GCTATTCTGC AATTATTTTT
    GAGACTCCCC TCCGAGTGGA CGTTGCAGTG CTGAAAGCGC GAGCGAAGCT GCTGCAGAAA
    TACCTGTGTG ACGAGCAGAA GGAGCTACAG GCGCTCTACG CCCTCCAGGC CCTTGTAGTG
    ACCTTAGAAC AGCCTCCCAA CCTGCTGCGG ATGTTCTTTG ACGCACTGTA TGACGAGGAC
    GTGGTGAAGG AGGATGCCTT CTACAGTTGG GAGAGTAGCA AGGACCCCGC TGAGCAGCAG
    GGCAAGGGTG TGGCCCTTAA ATCTGTCACA GCCTTCTTCA AGTGGCTCCG TGAAGCAGAG
    GAGGAGTCTG ACCACAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001181875.1
    CDS272..5092
    Translation

    Target ORF information:

    RefSeq Version NM_001194946.1
    Organism Homo sapiens(human)
    Definition Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001194946.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGTC CACAGGCCCC CCACCCGCCC CATCCCCCGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCGCCG GTGGTGTTCA GTACGCCACA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC
    CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGGCG TTCCACATAC
    GTTGTCCCGA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA
    AGCCCTACAG AATTTGGGAC CTACGCTGGC GCCTACTATC CAGCCCAAGG GGTGCAGCAG
    TTTCCCACTG GCGTGGCCCC CGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCC
    AAGAGGGAGC GTAAGACGAT CCGAATTCGA GATCCAAACC AAGGAGGAAA GGATATCACA
    GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CCCCTCCCCA GACGGGAGGC
    GGTCTGGAGC CTCAAGCTAA TGGGGAGACG CCCCAGGTTG CTGTCATTGT CCGGCCAGAT
    GACCGGTCAC AGGGAGCAAT CATTGCTGAC CGGCCAGGGC TGCCTGGCCC AGAGCATAGC
    CCTTCAGAAT CCCAGCCTTC GTCGCCTTCT CCGACCCCAT CACCATCCCC AGTCTTGGAA
    CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTATTCCTG GGGACACTAT GACAACTATA
    CAAATGTCTG TAGAAGAATC AACCCCCATC TCCCGTGAAA CTGGGGAGCC ATATCGCCTC
    TCTCCAGAAC CCACTCCTCT CGCCGAACCC ATACTGGAAG TAGAAGTGAC ACTTAGCAAA
    CCGGTTCCAG AATCTGAGTT TTCTTCCAGT CCTCTCCAGG CTCCCACCCC TTTGGCATCT
    CACACAGTGG AAATTCATGA GCCTAATGGC ATGGTCCCAT CTGAAGATCT GGAACCAGAG
    GTGGAGTCAA GCCCAGAGCT TGCTCCTCCC CCAGCTTGCC CCTCCGAATC CCCTGTGCCC
    ATTGCTCCAA CTGCCCAACC TGAGGAACTG CTCAACGGAG CCCCCTCGCC ACCAGCTGTG
    GACTTAAGCC CAGTCAGTGA GCCAGAGGAG CAGGCCAAGG AGGTGACAGC ATCAATGGCG
    CCCCCCACCA TCCCCTCTGC TACTCCAGCT ACGGCTCCTT CAGCTACTTC CCCAGCTCAG
    GAGGAGGAAA TGGAAGAAGA AGAAGAAGAG GAAGAAGGAG AAGCAGGAGA AGCAGGAGAA
    GCTGAGAGTG AGAAAGGAGG AGAGGAACTG CTCCCCCCAG AGAGTACCCC TATTCCAGCC
    AACTTGTCTC AGAATTTGGA GGCAGCAGCA GCCACTCAAG TGGCAGTATC TGTGCCAAAG
    AGGAGACGGA AAATTAAGGA GCTAAATAAG AAGGAGGCTG TTGGAGACCT TCTGGATGCC
    TTCAAGGAGG CGAACCCGGC AGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCAGCAAT
    CCAGGCCCAG AGTCTGAGGG CAGTGGTGTG CCCCCACGTC CTGAGGAAGC AGATGAGACC
    TGGGACTCAA AGGAAGACAA AATTCACAAT GCTGAGAACA TCCAGCCCGG GGAACAGAAG
    TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTAG AGGAGAAAAA ACGTTACGAC
    CGTGAGTTCC TGCTTGGTTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC AGAGGGATTG
    CCACATATCA GTGACGTGGT GCTGGACAAG GCCAATAAAA CACCACTGCG GCCACTGGAT
    CCCACTAGAC TACAAGGCAT AAATTGTGGC CCAGACTTCA CTCCATCCTT TGCCAACCTT
    GGCCGGACAA CCCTTAGCAC CCGTGGGCCC CCAAGGGGTG GGCCAGGTGG GGAGCTGCCC
    CGTGGGCCGG CTGGCCTGGG ACCCCGGCGC TCTCAGCAGG GACCCCGAAA AGAACCACGC
    AAGATCATTG CCACAGTGTT AATGACCGAA GATATAAAAC TGAACAAAGC AGAGAAAGCC
    TGGAAACCCA GCAGCAAGCG GACGGCGGCT GATAAGGATC GAGGGGAAGA AGATGCTGAT
    GGCAGCAAAA CCCAGGACCT ATTCCGCAGG GTGCGCTCCA TCCTGAATAA ACTGACACCC
    CAGATGTTCC AGCAGCTGAT GAAGCAAGTG ACGCAGCTGG CCATCGACAC CGAGGAACGC
    CTCAAAGGGG TCATTGACCT CATTTTTGAG AAGGCCATTT CAGAGCCCAA CTTCTCTGTG
    GCCTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC GGAAAAGCCA
    ACAGTGACTG TGAACTTCCG AAAGCTGTTG TTGAATCGAT GTCAGAAGGA GTTTGAGAAA
    GACAAAGATG ATGATGAGGT TTTTGAGAAG AAGCAAAAAG AGATGGATGA AGCTGCTACG
    GCAGAGGAAC GAGGACGCCT GAAGGAAGAG CTGGAAGAGG CTCGGGACAT AGCCCGGCGG
    CGCTCTTTAG GGAATATCAA GTTTATTGGA GAGTTGTTCA AACTGAAGAT GTTAACAGAG
    GCAATAATGC ATGACTGTGT GGTCAAACTG CTTAAGAACC ATGATGAAGA GTCCCTTGAG
    TGCCTTTGTC GTCTGCTCAC CACCATTGGC AAAGACCTGG ACTTTGAAAA AGCCAAGCCC
    CGAATGGATC AGTATTTCAA CCAGATGGAA AAAATCATTA AAGAAAAGAA GACGTCATCC
    CGCATCCGCT TTATGCTGCA GGACGTGCTG GATCTGCGAG GGAGCAATTG GGTGCCACGC
    CGAGGGGATC AGGGTCCCAA GACCATTGAC CAGATCCATA AGGAGGCTGA GATGGAAGAA
    CATCGAGAGC ACATCAAAGT GCAGCAGCTC ATGGCCAAGG GCAGTGACAA GCGTCGGGGC
    GGTCCTCCAG GCCCTCCCAT CAGCCGTGGA CTTCCCCTTG TGGATGATGG TGGCTGGAAC
    ACAGTTCCCA TCAGCAAAGG TAGCCGCCCC ATTGACACCT CACGACTCAC CAAGATCACC
    AAGCCTGGCT CCATCGATTC TAACAACCAG CTCTTTGCAC CTGGAGGGCG ACTGAGCTGG
    GGCAAGGGCA GCAGCGGAGG CTCAGGAGCC AAGCCCTCAG ACGCAGCATC AGAAGCTGCT
    CGCCCAGCTA CTAGTACTTT GAATCGCTTC TCAGCCCTTC AACAAGCGGT ACCCACAGAA
    AGCACAGATA ATAGACGTGT GGTGCAGAGG AGTAGCTTGA GCCGAGAACG AGGCGAGAAA
    GCTGGAGACC GAGGAGACCG CCTAGAGCGG AGTGAACGGG GAGGGGACCG TGGGGACCGG
    CTTGATCGTG CGCGGACACC TGCTACCAAG CGGAGCTTCA GCAAGGAAGT GGAGGAGCGG
    AGTAGAGAAC GGCCCTCCCA GCCTGAGGGG CTGCGCAAGG CAGCTAGCCT CACGGAGGAT
    CGGGACCGTG GGCGGGATGC CGTGAAGCGA GAAGCTGCCC TACCCCCAGT GAGCCCCCTG
    AAGGCGGCTC TCTCTGAGGA GGAGTTAGAG AAGAAATCCA AGGCTATCAT TGAGGAATAT
    CTCCATCTCA ATGACATGAA AGAGGCAGTC CAGTGCGTGC AGGAGCTGGC CTCACCCTCC
    TTGCTCTTCA TCTTTGTACG GCATGGTGTC GAGTCTACGC TGGAGCGCAG TGCCATTGCT
    CGTGAGCATA TGGGGCAGCT GCTGCACCAG CTGCTCTGTG CTGGGCATCT GTCTACTGCT
    CAGTACTACC AAGGGTTGTA TGAAATCTTG GAATTGGCTG AGGACATGGA AATTGACATC
    CCCCACGTGT GGCTCTACCT AGCGGAACTG GTAACACCCA TTCTGCAGGA AGGTGGGGTG
    CCCATGGGGG AGCTGTTCAG GGAGATTACA AAGCCTCTGA GACCGTTGGG CAAAGCTGCT
    TCCCTGTTGC TGGAGATCCT GGGCCTCCTG TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG
    ACGCTGTGGC GAGAAGCCGG GCTTAGCTGG AAGGAATTTC TACCTGAAGG CCAGGACATT
    GGTGCATTCG TCGCTGAACA GAAGGTGGAG TATACCCTGG GAGAGGAGTC GGAAGCCCCT
    GGCCAGAGGG CACTCCCCTC CGAGGAGCTG AACAGGCAGC TGGAGAAGCT GCTGAAGGAG
    GGCAGCAGTA ACCAGCGGGT GTTCGACTGG ATAGAGGCCA ACCTGAGTGA GCAGCAGATA
    GTATCCAACA CGTTAGTTCG AGCCCTCATG ACGGCTGTCT GCTATTCTGC AATTATTTTT
    GAGACTCCCC TCCGAGTGGA CGTTGCAGTG CTGAAAGCGC GAGCGAAGCT GCTGCAGAAA
    TACCTGTGTG ACGAGCAGAA GGAGCTACAG GCGCTCTACG CCCTCCAGGC CCTTGTAGTG
    ACCTTAGAAC AGCCTCCCAA CCTGCTGCGG ATGTTCTTTG ACGCACTGTA TGACGAGGAC
    GTGGTGAAGG AGGATGCCTT CTACAGTTGG GAGAGTAGCA AGGACCCCGC TGAGCAGCAG
    GGCAAGGGTG TGGCCCTTAA ATCTGTCACA GCCTTCTTCA AGTGGCTCCG TGAAGCAGAG
    GAGGAGTCTG ACCACAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13775
    Clone ID Related Accession (Same CDS sequence) NM_001194946.1 , NM_001194947.1
    Accession Version NM_001194947.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4821bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-19
    Organism Homo sapiens(human)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform 6
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR980540.1, BC140896.1 and BQ011010.1. Transcript Variant: This variant (7) differs in the 5' UTR, includes an additional in-frame exon in the 5' coding region, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (6) is longer than isoform 1. Both variants 6 and 7 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC140896.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGTC CACAGGCCCC CCACCCGCCC CATCCCCCGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCGCCG GTGGTGTTCA GTACGCCACA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC
    CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGGCG TTCCACATAC
    GTTGTCCCGA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA
    AGCCCTACAG AATTTGGGAC CTACGCTGGC GCCTACTATC CAGCCCAAGG GGTGCAGCAG
    TTTCCCACTG GCGTGGCCCC CGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCC
    AAGAGGGAGC GTAAGACGAT CCGAATTCGA GATCCAAACC AAGGAGGAAA GGATATCACA
    GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CCCCTCCCCA GACGGGAGGC
    GGTCTGGAGC CTCAAGCTAA TGGGGAGACG CCCCAGGTTG CTGTCATTGT CCGGCCAGAT
    GACCGGTCAC AGGGAGCAAT CATTGCTGAC CGGCCAGGGC TGCCTGGCCC AGAGCATAGC
    CCTTCAGAAT CCCAGCCTTC GTCGCCTTCT CCGACCCCAT CACCATCCCC AGTCTTGGAA
    CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTATTCCTG GGGACACTAT GACAACTATA
    CAAATGTCTG TAGAAGAATC AACCCCCATC TCCCGTGAAA CTGGGGAGCC ATATCGCCTC
    TCTCCAGAAC CCACTCCTCT CGCCGAACCC ATACTGGAAG TAGAAGTGAC ACTTAGCAAA
    CCGGTTCCAG AATCTGAGTT TTCTTCCAGT CCTCTCCAGG CTCCCACCCC TTTGGCATCT
    CACACAGTGG AAATTCATGA GCCTAATGGC ATGGTCCCAT CTGAAGATCT GGAACCAGAG
    GTGGAGTCAA GCCCAGAGCT TGCTCCTCCC CCAGCTTGCC CCTCCGAATC CCCTGTGCCC
    ATTGCTCCAA CTGCCCAACC TGAGGAACTG CTCAACGGAG CCCCCTCGCC ACCAGCTGTG
    GACTTAAGCC CAGTCAGTGA GCCAGAGGAG CAGGCCAAGG AGGTGACAGC ATCAATGGCG
    CCCCCCACCA TCCCCTCTGC TACTCCAGCT ACGGCTCCTT CAGCTACTTC CCCAGCTCAG
    GAGGAGGAAA TGGAAGAAGA AGAAGAAGAG GAAGAAGGAG AAGCAGGAGA AGCAGGAGAA
    GCTGAGAGTG AGAAAGGAGG AGAGGAACTG CTCCCCCCAG AGAGTACCCC TATTCCAGCC
    AACTTGTCTC AGAATTTGGA GGCAGCAGCA GCCACTCAAG TGGCAGTATC TGTGCCAAAG
    AGGAGACGGA AAATTAAGGA GCTAAATAAG AAGGAGGCTG TTGGAGACCT TCTGGATGCC
    TTCAAGGAGG CGAACCCGGC AGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCAGCAAT
    CCAGGCCCAG AGTCTGAGGG CAGTGGTGTG CCCCCACGTC CTGAGGAAGC AGATGAGACC
    TGGGACTCAA AGGAAGACAA AATTCACAAT GCTGAGAACA TCCAGCCCGG GGAACAGAAG
    TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTAG AGGAGAAAAA ACGTTACGAC
    CGTGAGTTCC TGCTTGGTTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC AGAGGGATTG
    CCACATATCA GTGACGTGGT GCTGGACAAG GCCAATAAAA CACCACTGCG GCCACTGGAT
    CCCACTAGAC TACAAGGCAT AAATTGTGGC CCAGACTTCA CTCCATCCTT TGCCAACCTT
    GGCCGGACAA CCCTTAGCAC CCGTGGGCCC CCAAGGGGTG GGCCAGGTGG GGAGCTGCCC
    CGTGGGCCGG CTGGCCTGGG ACCCCGGCGC TCTCAGCAGG GACCCCGAAA AGAACCACGC
    AAGATCATTG CCACAGTGTT AATGACCGAA GATATAAAAC TGAACAAAGC AGAGAAAGCC
    TGGAAACCCA GCAGCAAGCG GACGGCGGCT GATAAGGATC GAGGGGAAGA AGATGCTGAT
    GGCAGCAAAA CCCAGGACCT ATTCCGCAGG GTGCGCTCCA TCCTGAATAA ACTGACACCC
    CAGATGTTCC AGCAGCTGAT GAAGCAAGTG ACGCAGCTGG CCATCGACAC CGAGGAACGC
    CTCAAAGGGG TCATTGACCT CATTTTTGAG AAGGCCATTT CAGAGCCCAA CTTCTCTGTG
    GCCTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC GGAAAAGCCA
    ACAGTGACTG TGAACTTCCG AAAGCTGTTG TTGAATCGAT GTCAGAAGGA GTTTGAGAAA
    GACAAAGATG ATGATGAGGT TTTTGAGAAG AAGCAAAAAG AGATGGATGA AGCTGCTACG
    GCAGAGGAAC GAGGACGCCT GAAGGAAGAG CTGGAAGAGG CTCGGGACAT AGCCCGGCGG
    CGCTCTTTAG GGAATATCAA GTTTATTGGA GAGTTGTTCA AACTGAAGAT GTTAACAGAG
    GCAATAATGC ATGACTGTGT GGTCAAACTG CTTAAGAACC ATGATGAAGA GTCCCTTGAG
    TGCCTTTGTC GTCTGCTCAC CACCATTGGC AAAGACCTGG ACTTTGAAAA AGCCAAGCCC
    CGAATGGATC AGTATTTCAA CCAGATGGAA AAAATCATTA AAGAAAAGAA GACGTCATCC
    CGCATCCGCT TTATGCTGCA GGACGTGCTG GATCTGCGAG GGAGCAATTG GGTGCCACGC
    CGAGGGGATC AGGGTCCCAA GACCATTGAC CAGATCCATA AGGAGGCTGA GATGGAAGAA
    CATCGAGAGC ACATCAAAGT GCAGCAGCTC ATGGCCAAGG GCAGTGACAA GCGTCGGGGC
    GGTCCTCCAG GCCCTCCCAT CAGCCGTGGA CTTCCCCTTG TGGATGATGG TGGCTGGAAC
    ACAGTTCCCA TCAGCAAAGG TAGCCGCCCC ATTGACACCT CACGACTCAC CAAGATCACC
    AAGCCTGGCT CCATCGATTC TAACAACCAG CTCTTTGCAC CTGGAGGGCG ACTGAGCTGG
    GGCAAGGGCA GCAGCGGAGG CTCAGGAGCC AAGCCCTCAG ACGCAGCATC AGAAGCTGCT
    CGCCCAGCTA CTAGTACTTT GAATCGCTTC TCAGCCCTTC AACAAGCGGT ACCCACAGAA
    AGCACAGATA ATAGACGTGT GGTGCAGAGG AGTAGCTTGA GCCGAGAACG AGGCGAGAAA
    GCTGGAGACC GAGGAGACCG CCTAGAGCGG AGTGAACGGG GAGGGGACCG TGGGGACCGG
    CTTGATCGTG CGCGGACACC TGCTACCAAG CGGAGCTTCA GCAAGGAAGT GGAGGAGCGG
    AGTAGAGAAC GGCCCTCCCA GCCTGAGGGG CTGCGCAAGG CAGCTAGCCT CACGGAGGAT
    CGGGACCGTG GGCGGGATGC CGTGAAGCGA GAAGCTGCCC TACCCCCAGT GAGCCCCCTG
    AAGGCGGCTC TCTCTGAGGA GGAGTTAGAG AAGAAATCCA AGGCTATCAT TGAGGAATAT
    CTCCATCTCA ATGACATGAA AGAGGCAGTC CAGTGCGTGC AGGAGCTGGC CTCACCCTCC
    TTGCTCTTCA TCTTTGTACG GCATGGTGTC GAGTCTACGC TGGAGCGCAG TGCCATTGCT
    CGTGAGCATA TGGGGCAGCT GCTGCACCAG CTGCTCTGTG CTGGGCATCT GTCTACTGCT
    CAGTACTACC AAGGGTTGTA TGAAATCTTG GAATTGGCTG AGGACATGGA AATTGACATC
    CCCCACGTGT GGCTCTACCT AGCGGAACTG GTAACACCCA TTCTGCAGGA AGGTGGGGTG
    CCCATGGGGG AGCTGTTCAG GGAGATTACA AAGCCTCTGA GACCGTTGGG CAAAGCTGCT
    TCCCTGTTGC TGGAGATCCT GGGCCTCCTG TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG
    ACGCTGTGGC GAGAAGCCGG GCTTAGCTGG AAGGAATTTC TACCTGAAGG CCAGGACATT
    GGTGCATTCG TCGCTGAACA GAAGGTGGAG TATACCCTGG GAGAGGAGTC GGAAGCCCCT
    GGCCAGAGGG CACTCCCCTC CGAGGAGCTG AACAGGCAGC TGGAGAAGCT GCTGAAGGAG
    GGCAGCAGTA ACCAGCGGGT GTTCGACTGG ATAGAGGCCA ACCTGAGTGA GCAGCAGATA
    GTATCCAACA CGTTAGTTCG AGCCCTCATG ACGGCTGTCT GCTATTCTGC AATTATTTTT
    GAGACTCCCC TCCGAGTGGA CGTTGCAGTG CTGAAAGCGC GAGCGAAGCT GCTGCAGAAA
    TACCTGTGTG ACGAGCAGAA GGAGCTACAG GCGCTCTACG CCCTCCAGGC CCTTGTAGTG
    ACCTTAGAAC AGCCTCCCAA CCTGCTGCGG ATGTTCTTTG ACGCACTGTA TGACGAGGAC
    GTGGTGAAGG AGGATGCCTT CTACAGTTGG GAGAGTAGCA AGGACCCCGC TGAGCAGCAG
    GGCAAGGGTG TGGCCCTTAA ATCTGTCACA GCCTTCTTCA AGTGGCTCCG TGAAGCAGAG
    GAGGAGTCTG ACCACAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001181876.1
    CDS215..5035
    Translation

    Target ORF information:

    RefSeq Version NM_001194947.1
    Organism Homo sapiens(human)
    Definition Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001194947.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGTC CACAGGCCCC CCACCCGCCC CATCCCCCGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCGCCG GTGGTGTTCA GTACGCCACA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC
    CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGGCG TTCCACATAC
    GTTGTCCCGA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA
    AGCCCTACAG AATTTGGGAC CTACGCTGGC GCCTACTATC CAGCCCAAGG GGTGCAGCAG
    TTTCCCACTG GCGTGGCCCC CGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCC
    AAGAGGGAGC GTAAGACGAT CCGAATTCGA GATCCAAACC AAGGAGGAAA GGATATCACA
    GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CCCCTCCCCA GACGGGAGGC
    GGTCTGGAGC CTCAAGCTAA TGGGGAGACG CCCCAGGTTG CTGTCATTGT CCGGCCAGAT
    GACCGGTCAC AGGGAGCAAT CATTGCTGAC CGGCCAGGGC TGCCTGGCCC AGAGCATAGC
    CCTTCAGAAT CCCAGCCTTC GTCGCCTTCT CCGACCCCAT CACCATCCCC AGTCTTGGAA
    CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTATTCCTG GGGACACTAT GACAACTATA
    CAAATGTCTG TAGAAGAATC AACCCCCATC TCCCGTGAAA CTGGGGAGCC ATATCGCCTC
    TCTCCAGAAC CCACTCCTCT CGCCGAACCC ATACTGGAAG TAGAAGTGAC ACTTAGCAAA
    CCGGTTCCAG AATCTGAGTT TTCTTCCAGT CCTCTCCAGG CTCCCACCCC TTTGGCATCT
    CACACAGTGG AAATTCATGA GCCTAATGGC ATGGTCCCAT CTGAAGATCT GGAACCAGAG
    GTGGAGTCAA GCCCAGAGCT TGCTCCTCCC CCAGCTTGCC CCTCCGAATC CCCTGTGCCC
    ATTGCTCCAA CTGCCCAACC TGAGGAACTG CTCAACGGAG CCCCCTCGCC ACCAGCTGTG
    GACTTAAGCC CAGTCAGTGA GCCAGAGGAG CAGGCCAAGG AGGTGACAGC ATCAATGGCG
    CCCCCCACCA TCCCCTCTGC TACTCCAGCT ACGGCTCCTT CAGCTACTTC CCCAGCTCAG
    GAGGAGGAAA TGGAAGAAGA AGAAGAAGAG GAAGAAGGAG AAGCAGGAGA AGCAGGAGAA
    GCTGAGAGTG AGAAAGGAGG AGAGGAACTG CTCCCCCCAG AGAGTACCCC TATTCCAGCC
    AACTTGTCTC AGAATTTGGA GGCAGCAGCA GCCACTCAAG TGGCAGTATC TGTGCCAAAG
    AGGAGACGGA AAATTAAGGA GCTAAATAAG AAGGAGGCTG TTGGAGACCT TCTGGATGCC
    TTCAAGGAGG CGAACCCGGC AGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCAGCAAT
    CCAGGCCCAG AGTCTGAGGG CAGTGGTGTG CCCCCACGTC CTGAGGAAGC AGATGAGACC
    TGGGACTCAA AGGAAGACAA AATTCACAAT GCTGAGAACA TCCAGCCCGG GGAACAGAAG
    TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTAG AGGAGAAAAA ACGTTACGAC
    CGTGAGTTCC TGCTTGGTTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC AGAGGGATTG
    CCACATATCA GTGACGTGGT GCTGGACAAG GCCAATAAAA CACCACTGCG GCCACTGGAT
    CCCACTAGAC TACAAGGCAT AAATTGTGGC CCAGACTTCA CTCCATCCTT TGCCAACCTT
    GGCCGGACAA CCCTTAGCAC CCGTGGGCCC CCAAGGGGTG GGCCAGGTGG GGAGCTGCCC
    CGTGGGCCGG CTGGCCTGGG ACCCCGGCGC TCTCAGCAGG GACCCCGAAA AGAACCACGC
    AAGATCATTG CCACAGTGTT AATGACCGAA GATATAAAAC TGAACAAAGC AGAGAAAGCC
    TGGAAACCCA GCAGCAAGCG GACGGCGGCT GATAAGGATC GAGGGGAAGA AGATGCTGAT
    GGCAGCAAAA CCCAGGACCT ATTCCGCAGG GTGCGCTCCA TCCTGAATAA ACTGACACCC
    CAGATGTTCC AGCAGCTGAT GAAGCAAGTG ACGCAGCTGG CCATCGACAC CGAGGAACGC
    CTCAAAGGGG TCATTGACCT CATTTTTGAG AAGGCCATTT CAGAGCCCAA CTTCTCTGTG
    GCCTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC GGAAAAGCCA
    ACAGTGACTG TGAACTTCCG AAAGCTGTTG TTGAATCGAT GTCAGAAGGA GTTTGAGAAA
    GACAAAGATG ATGATGAGGT TTTTGAGAAG AAGCAAAAAG AGATGGATGA AGCTGCTACG
    GCAGAGGAAC GAGGACGCCT GAAGGAAGAG CTGGAAGAGG CTCGGGACAT AGCCCGGCGG
    CGCTCTTTAG GGAATATCAA GTTTATTGGA GAGTTGTTCA AACTGAAGAT GTTAACAGAG
    GCAATAATGC ATGACTGTGT GGTCAAACTG CTTAAGAACC ATGATGAAGA GTCCCTTGAG
    TGCCTTTGTC GTCTGCTCAC CACCATTGGC AAAGACCTGG ACTTTGAAAA AGCCAAGCCC
    CGAATGGATC AGTATTTCAA CCAGATGGAA AAAATCATTA AAGAAAAGAA GACGTCATCC
    CGCATCCGCT TTATGCTGCA GGACGTGCTG GATCTGCGAG GGAGCAATTG GGTGCCACGC
    CGAGGGGATC AGGGTCCCAA GACCATTGAC CAGATCCATA AGGAGGCTGA GATGGAAGAA
    CATCGAGAGC ACATCAAAGT GCAGCAGCTC ATGGCCAAGG GCAGTGACAA GCGTCGGGGC
    GGTCCTCCAG GCCCTCCCAT CAGCCGTGGA CTTCCCCTTG TGGATGATGG TGGCTGGAAC
    ACAGTTCCCA TCAGCAAAGG TAGCCGCCCC ATTGACACCT CACGACTCAC CAAGATCACC
    AAGCCTGGCT CCATCGATTC TAACAACCAG CTCTTTGCAC CTGGAGGGCG ACTGAGCTGG
    GGCAAGGGCA GCAGCGGAGG CTCAGGAGCC AAGCCCTCAG ACGCAGCATC AGAAGCTGCT
    CGCCCAGCTA CTAGTACTTT GAATCGCTTC TCAGCCCTTC AACAAGCGGT ACCCACAGAA
    AGCACAGATA ATAGACGTGT GGTGCAGAGG AGTAGCTTGA GCCGAGAACG AGGCGAGAAA
    GCTGGAGACC GAGGAGACCG CCTAGAGCGG AGTGAACGGG GAGGGGACCG TGGGGACCGG
    CTTGATCGTG CGCGGACACC TGCTACCAAG CGGAGCTTCA GCAAGGAAGT GGAGGAGCGG
    AGTAGAGAAC GGCCCTCCCA GCCTGAGGGG CTGCGCAAGG CAGCTAGCCT CACGGAGGAT
    CGGGACCGTG GGCGGGATGC CGTGAAGCGA GAAGCTGCCC TACCCCCAGT GAGCCCCCTG
    AAGGCGGCTC TCTCTGAGGA GGAGTTAGAG AAGAAATCCA AGGCTATCAT TGAGGAATAT
    CTCCATCTCA ATGACATGAA AGAGGCAGTC CAGTGCGTGC AGGAGCTGGC CTCACCCTCC
    TTGCTCTTCA TCTTTGTACG GCATGGTGTC GAGTCTACGC TGGAGCGCAG TGCCATTGCT
    CGTGAGCATA TGGGGCAGCT GCTGCACCAG CTGCTCTGTG CTGGGCATCT GTCTACTGCT
    CAGTACTACC AAGGGTTGTA TGAAATCTTG GAATTGGCTG AGGACATGGA AATTGACATC
    CCCCACGTGT GGCTCTACCT AGCGGAACTG GTAACACCCA TTCTGCAGGA AGGTGGGGTG
    CCCATGGGGG AGCTGTTCAG GGAGATTACA AAGCCTCTGA GACCGTTGGG CAAAGCTGCT
    TCCCTGTTGC TGGAGATCCT GGGCCTCCTG TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG
    ACGCTGTGGC GAGAAGCCGG GCTTAGCTGG AAGGAATTTC TACCTGAAGG CCAGGACATT
    GGTGCATTCG TCGCTGAACA GAAGGTGGAG TATACCCTGG GAGAGGAGTC GGAAGCCCCT
    GGCCAGAGGG CACTCCCCTC CGAGGAGCTG AACAGGCAGC TGGAGAAGCT GCTGAAGGAG
    GGCAGCAGTA ACCAGCGGGT GTTCGACTGG ATAGAGGCCA ACCTGAGTGA GCAGCAGATA
    GTATCCAACA CGTTAGTTCG AGCCCTCATG ACGGCTGTCT GCTATTCTGC AATTATTTTT
    GAGACTCCCC TCCGAGTGGA CGTTGCAGTG CTGAAAGCGC GAGCGAAGCT GCTGCAGAAA
    TACCTGTGTG ACGAGCAGAA GGAGCTACAG GCGCTCTACG CCCTCCAGGC CCTTGTAGTG
    ACCTTAGAAC AGCCTCCCAA CCTGCTGCGG ATGTTCTTTG ACGCACTGTA TGACGAGGAC
    GTGGTGAAGG AGGATGCCTT CTACAGTTGG GAGAGTAGCA AGGACCCCGC TGAGCAGCAG
    GGCAAGGGTG TGGCCCTTAA ATCTGTCACA GCCTTCTTCA AGTGGCTCCG TGAAGCAGAG
    GAGGAGTCTG ACCACAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13776
    Clone ID Related Accession (Same CDS sequence) NM_004953.4 , NM_004953.5
    Accession Version NM_004953.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4215bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20 and BQ011010.1. On Aug 13, 2010 this sequence version replaced NM_004953.3. Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream start codon, compared to variant 1. The resulting isoform (4) is shorter at the N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D12686.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCTGGGG CCCGCACTGC CTCCACACCC ACCCCTCCCC AGACGGGAGG CGGTCTGGAG 
    CCTCAAGCTA ATGGGGAGAC GCCCCAGGTT GCTGTCATTG TCCGGCCAGA TGACCGGTCA
    CAGGGAGCAA TCATTGCTGA CCGGCCAGGG CTGCCTGGCC CAGAGCATAG CCCTTCAGAA
    TCCCAGCCTT CGTCGCCTTC TCCGACCCCA TCACCATCCC CAGTCTTGGA ACCGGGGTCT
    GAGCCTAATC TCGCAGTCCT CTCTATTCCT GGGGACACTA TGACAACTAT ACAAATGTCT
    GTAGAAGAAT CAACCCCCAT CTCCCGTGAA ACTGGGGAGC CATATCGCCT CTCTCCAGAA
    CCCACTCCTC TCGCCGAACC CATACTGGAA GTAGAAGTGA CACTTAGCAA ACCGGTTCCA
    GAATCTGAGT TTTCTTCCAG TCCTCTCCAG GCTCCCACCC CTTTGGCATC TCACACAGTG
    GAAATTCATG AGCCTAATGG CATGGTCCCA TCTGAAGATC TGGAACCAGA GGTGGAGTCA
    AGCCCAGAGC TTGCTCCTCC CCCAGCTTGC CCCTCCGAAT CCCCTGTGCC CATTGCTCCA
    ACTGCCCAAC CTGAGGAACT GCTCAACGGA GCCCCCTCGC CACCAGCTGT GGACTTAAGC
    CCAGTCAGTG AGCCAGAGGA GCAGGCCAAG GAGGTGACAG CATCAATGGC GCCCCCCACC
    ATCCCCTCTG CTACTCCAGC TACGGCTCCT TCAGCTACTT CCCCAGCTCA GGAGGAGGAA
    ATGGAAGAAG AAGAAGAAGA GGAAGAAGGA GAAGCAGGAG AAGCAGGAGA AGCTGAGAGT
    GAGAAAGGAG GAGAGGAACT GCTCCCCCCA GAGAGTACCC CTATTCCAGC CAACTTGTCT
    CAGAATTTGG AGGCAGCAGC AGCCACTCAA GTGGCAGTAT CTGTGCCAAA GAGGAGACGG
    AAAATTAAGG AGCTAAATAA GAAGGAGGCT GTTGGAGACC TTCTGGATGC CTTCAAGGAG
    GCGAACCCGG CAGTACCAGA GGTGGAAAAT CAGCCTCCTG CAGGCAGCAA TCCAGGCCCA
    GAGTCTGAGG GCAGTGGTGT GCCCCCACGT CCTGAGGAAG CAGATGAGAC CTGGGACTCA
    AAGGAAGACA AAATTCACAA TGCTGAGAAC ATCCAGCCCG GGGAACAGAA GTATGAATAT
    AAGTCAGATC AGTGGAAGCC TCTAAACCTA GAGGAGAAAA AACGTTACGA CCGTGAGTTC
    CTGCTTGGTT TTCAGTTCAT CTTTGCCAGT ATGCAGAAGC CAGAGGGATT GCCACATATC
    AGTGACGTGG TGCTGGACAA GGCCAATAAA ACACCACTGC GGCCACTGGA TCCCACTAGA
    CTACAAGGCA TAAATTGTGG CCCAGACTTC ACTCCATCCT TTGCCAACCT TGGCCGGACA
    ACCCTTAGCA CCCGTGGGCC CCCAAGGGGT GGGCCAGGTG GGGAGCTGCC CCGTGGGCCG
    CAGGCTGGCC TGGGACCCCG GCGCTCTCAG CAGGGACCCC GAAAAGAACC ACGCAAGATC
    ATTGCCACAG TGTTAATGAC CGAAGATATA AAACTGAACA AAGCAGAGAA AGCCTGGAAA
    CCCAGCAGCA AGCGGACGGC GGCTGATAAG GATCGAGGGG AAGAAGATGC TGATGGCAGC
    AAAACCCAGG ACCTATTCCG CAGGGTGCGC TCCATCCTGA ATAAACTGAC ACCCCAGATG
    TTCCAGCAGC TGATGAAGCA AGTGACGCAG CTGGCCATCG ACACCGAGGA ACGCCTCAAA
    GGGGTCATTG ACCTCATTTT TGAGAAGGCC ATTTCAGAGC CCAACTTCTC TGTGGCCTAT
    GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACGGAAAA GCCAACAGTG
    ACTGTGAACT TCCGAAAGCT GTTGTTGAAT CGATGTCAGA AGGAGTTTGA GAAAGACAAA
    GATGATGATG AGGTTTTTGA GAAGAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAG
    GAACGAGGAC GCCTGAAGGA AGAGCTGGAA GAGGCTCGGG ACATAGCCCG GCGGCGCTCT
    TTAGGGAATA TCAAGTTTAT TGGAGAGTTG TTCAAACTGA AGATGTTAAC AGAGGCAATA
    ATGCATGACT GTGTGGTCAA ACTGCTTAAG AACCATGATG AAGAGTCCCT TGAGTGCCTT
    TGTCGTCTGC TCACCACCAT TGGCAAAGAC CTGGACTTTG AAAAAGCCAA GCCCCGAATG
    GATCAGTATT TCAACCAGAT GGAAAAAATC ATTAAAGAAA AGAAGACGTC ATCCCGCATC
    CGCTTTATGC TGCAGGACGT GCTGGATCTG CGAGGGAGCA ATTGGGTGCC ACGCCGAGGG
    GATCAGGGTC CCAAGACCAT TGACCAGATC CATAAGGAGG CTGAGATGGA AGAACATCGA
    GAGCACATCA AAGTGCAGCA GCTCATGGCC AAGGGCAGTG ACAAGCGTCG GGGCGGTCCT
    CCAGGCCCTC CCATCAGCCG TGGACTTCCC CTTGTGGATG ATGGTGGCTG GAACACAGTT
    CCCATCAGCA AAGGTAGCCG CCCCATTGAC ACCTCACGAC TCACCAAGAT CACCAAGCCT
    GGCTCCATCG ATTCTAACAA CCAGCTCTTT GCACCTGGAG GGCGACTGAG CTGGGGCAAG
    GGCAGCAGCG GAGGCTCAGG AGCCAAGCCC TCAGACGCAG CATCAGAAGC TGCTCGCCCA
    GCTACTAGTA CTTTGAATCG CTTCTCAGCC CTTCAACAAG CGGTACCCAC AGAAAGCACA
    GATAATAGAC GTGTGGTGCA GAGGAGTAGC TTGAGCCGAG AACGAGGCGA GAAAGCTGGA
    GACCGAGGAG ACCGCCTAGA GCGGAGTGAA CGGGGAGGGG ACCGTGGGGA CCGGCTTGAT
    CGTGCGCGGA CACCTGCTAC CAAGCGGAGC TTCAGCAAGG AAGTGGAGGA GCGGAGTAGA
    GAACGGCCCT CCCAGCCTGA GGGGCTGCGC AAGGCAGCTA GCCTCACGGA GGATCGGGAC
    CGTGGGCGGG ATGCCGTGAA GCGAGAAGCT GCCCTACCCC CAGTGAGCCC CCTGAAGGCG
    GCTCTCTCTG AGGAGGAGTT AGAGAAGAAA TCCAAGGCTA TCATTGAGGA ATATCTCCAT
    CTCAATGACA TGAAAGAGGC AGTCCAGTGC GTGCAGGAGC TGGCCTCACC CTCCTTGCTC
    TTCATCTTTG TACGGCATGG TGTCGAGTCT ACGCTGGAGC GCAGTGCCAT TGCTCGTGAG
    CATATGGGGC AGCTGCTGCA CCAGCTGCTC TGTGCTGGGC ATCTGTCTAC TGCTCAGTAC
    TACCAAGGGT TGTATGAAAT CTTGGAATTG GCTGAGGACA TGGAAATTGA CATCCCCCAC
    GTGTGGCTCT ACCTAGCGGA ACTGGTAACA CCCATTCTGC AGGAAGGTGG GGTGCCCATG
    GGGGAGCTGT TCAGGGAGAT TACAAAGCCT CTGAGACCGT TGGGCAAAGC TGCTTCCCTG
    TTGCTGGAGA TCCTGGGCCT CCTGTGCAAA AGCATGGGTC CTAAAAAGGT GGGGACGCTG
    TGGCGAGAAG CCGGGCTTAG CTGGAAGGAA TTTCTACCTG AAGGCCAGGA CATTGGTGCA
    TTCGTCGCTG AACAGAAGGT GGAGTATACC CTGGGAGAGG AGTCGGAAGC CCCTGGCCAG
    AGGGCACTCC CCTCCGAGGA GCTGAACAGG CAGCTGGAGA AGCTGCTGAA GGAGGGCAGC
    AGTAACCAGC GGGTGTTCGA CTGGATAGAG GCCAACCTGA GTGAGCAGCA GATAGTATCC
    AACACGTTAG TTCGAGCCCT CATGACGGCT GTCTGCTATT CTGCAATTAT TTTTGAGACT
    CCCCTCCGAG TGGACGTTGC AGTGCTGAAA GCGCGAGCGA AGCTGCTGCA GAAATACCTG
    TGTGACGAGC AGAAGGAGCT ACAGGCGCTC TACGCCCTCC AGGCCCTTGT AGTGACCTTA
    GAACAGCCTC CCAACCTGCT GCGGATGTTC TTTGACGCAC TGTATGACGA GGACGTGGTG
    AAGGAGGATG CCTTCTACAG TTGGGAGAGT AGCAAGGACC CCGCTGAGCA GCAGGGCAAG
    GGTGTGGCCC TTAAATCTGT CACAGCCTTC TTCAAGTGGC TCCGTGAAGC AGAGGAGGAG
    TCTGACCACA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004944.3
    CDS369..4583
    Translation

    Target ORF information:

    RefSeq Version NM_004953.4
    Organism Homo sapiens(human)
    Definition Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004953.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCTGGGG CCCGCACTGC CTCCACACCC ACCCCTCCCC AGACGGGAGG CGGTCTGGAG 
    CCTCAAGCTA ATGGGGAGAC GCCCCAGGTT GCTGTCATTG TCCGGCCAGA TGACCGGTCA
    CAGGGAGCAA TCATTGCTGA CCGGCCAGGG CTGCCTGGCC CAGAGCATAG CCCTTCAGAA
    TCCCAGCCTT CGTCGCCTTC TCCGACCCCA TCACCATCCC CAGTCTTGGA ACCGGGGTCT
    GAGCCTAATC TCGCAGTCCT CTCTATTCCT GGGGACACTA TGACAACTAT ACAAATGTCT
    GTAGAAGAAT CAACCCCCAT CTCCCGTGAA ACTGGGGAGC CATATCGCCT CTCTCCAGAA
    CCCACTCCTC TCGCCGAACC CATACTGGAA GTAGAAGTGA CACTTAGCAA ACCGGTTCCA
    GAATCTGAGT TTTCTTCCAG TCCTCTCCAG GCTCCCACCC CTTTGGCATC TCACACAGTG
    GAAATTCATG AGCCTAATGG CATGGTCCCA TCTGAAGATC TGGAACCAGA GGTGGAGTCA
    AGCCCAGAGC TTGCTCCTCC CCCAGCTTGC CCCTCCGAAT CCCCTGTGCC CATTGCTCCA
    ACTGCCCAAC CTGAGGAACT GCTCAACGGA GCCCCCTCGC CACCAGCTGT GGACTTAAGC
    CCAGTCAGTG AGCCAGAGGA GCAGGCCAAG GAGGTGACAG CATCAATGGC GCCCCCCACC
    ATCCCCTCTG CTACTCCAGC TACGGCTCCT TCAGCTACTT CCCCAGCTCA GGAGGAGGAA
    ATGGAAGAAG AAGAAGAAGA GGAAGAAGGA GAAGCAGGAG AAGCAGGAGA AGCTGAGAGT
    GAGAAAGGAG GAGAGGAACT GCTCCCCCCA GAGAGTACCC CTATTCCAGC CAACTTGTCT
    CAGAATTTGG AGGCAGCAGC AGCCACTCAA GTGGCAGTAT CTGTGCCAAA GAGGAGACGG
    AAAATTAAGG AGCTAAATAA GAAGGAGGCT GTTGGAGACC TTCTGGATGC CTTCAAGGAG
    GCGAACCCGG CAGTACCAGA GGTGGAAAAT CAGCCTCCTG CAGGCAGCAA TCCAGGCCCA
    GAGTCTGAGG GCAGTGGTGT GCCCCCACGT CCTGAGGAAG CAGATGAGAC CTGGGACTCA
    AAGGAAGACA AAATTCACAA TGCTGAGAAC ATCCAGCCCG GGGAACAGAA GTATGAATAT
    AAGTCAGATC AGTGGAAGCC TCTAAACCTA GAGGAGAAAA AACGTTACGA CCGTGAGTTC
    CTGCTTGGTT TTCAGTTCAT CTTTGCCAGT ATGCAGAAGC CAGAGGGATT GCCACATATC
    AGTGACGTGG TGCTGGACAA GGCCAATAAA ACACCACTGC GGCCACTGGA TCCCACTAGA
    CTACAAGGCA TAAATTGTGG CCCAGACTTC ACTCCATCCT TTGCCAACCT TGGCCGGACA
    ACCCTTAGCA CCCGTGGGCC CCCAAGGGGT GGGCCAGGTG GGGAGCTGCC CCGTGGGCCG
    CAGGCTGGCC TGGGACCCCG GCGCTCTCAG CAGGGACCCC GAAAAGAACC ACGCAAGATC
    ATTGCCACAG TGTTAATGAC CGAAGATATA AAACTGAACA AAGCAGAGAA AGCCTGGAAA
    CCCAGCAGCA AGCGGACGGC GGCTGATAAG GATCGAGGGG AAGAAGATGC TGATGGCAGC
    AAAACCCAGG ACCTATTCCG CAGGGTGCGC TCCATCCTGA ATAAACTGAC ACCCCAGATG
    TTCCAGCAGC TGATGAAGCA AGTGACGCAG CTGGCCATCG ACACCGAGGA ACGCCTCAAA
    GGGGTCATTG ACCTCATTTT TGAGAAGGCC ATTTCAGAGC CCAACTTCTC TGTGGCCTAT
    GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACGGAAAA GCCAACAGTG
    ACTGTGAACT TCCGAAAGCT GTTGTTGAAT CGATGTCAGA AGGAGTTTGA GAAAGACAAA
    GATGATGATG AGGTTTTTGA GAAGAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAG
    GAACGAGGAC GCCTGAAGGA AGAGCTGGAA GAGGCTCGGG ACATAGCCCG GCGGCGCTCT
    TTAGGGAATA TCAAGTTTAT TGGAGAGTTG TTCAAACTGA AGATGTTAAC AGAGGCAATA
    ATGCATGACT GTGTGGTCAA ACTGCTTAAG AACCATGATG AAGAGTCCCT TGAGTGCCTT
    TGTCGTCTGC TCACCACCAT TGGCAAAGAC CTGGACTTTG AAAAAGCCAA GCCCCGAATG
    GATCAGTATT TCAACCAGAT GGAAAAAATC ATTAAAGAAA AGAAGACGTC ATCCCGCATC
    CGCTTTATGC TGCAGGACGT GCTGGATCTG CGAGGGAGCA ATTGGGTGCC ACGCCGAGGG
    GATCAGGGTC CCAAGACCAT TGACCAGATC CATAAGGAGG CTGAGATGGA AGAACATCGA
    GAGCACATCA AAGTGCAGCA GCTCATGGCC AAGGGCAGTG ACAAGCGTCG GGGCGGTCCT
    CCAGGCCCTC CCATCAGCCG TGGACTTCCC CTTGTGGATG ATGGTGGCTG GAACACAGTT
    CCCATCAGCA AAGGTAGCCG CCCCATTGAC ACCTCACGAC TCACCAAGAT CACCAAGCCT
    GGCTCCATCG ATTCTAACAA CCAGCTCTTT GCACCTGGAG GGCGACTGAG CTGGGGCAAG
    GGCAGCAGCG GAGGCTCAGG AGCCAAGCCC TCAGACGCAG CATCAGAAGC TGCTCGCCCA
    GCTACTAGTA CTTTGAATCG CTTCTCAGCC CTTCAACAAG CGGTACCCAC AGAAAGCACA
    GATAATAGAC GTGTGGTGCA GAGGAGTAGC TTGAGCCGAG AACGAGGCGA GAAAGCTGGA
    GACCGAGGAG ACCGCCTAGA GCGGAGTGAA CGGGGAGGGG ACCGTGGGGA CCGGCTTGAT
    CGTGCGCGGA CACCTGCTAC CAAGCGGAGC TTCAGCAAGG AAGTGGAGGA GCGGAGTAGA
    GAACGGCCCT CCCAGCCTGA GGGGCTGCGC AAGGCAGCTA GCCTCACGGA GGATCGGGAC
    CGTGGGCGGG ATGCCGTGAA GCGAGAAGCT GCCCTACCCC CAGTGAGCCC CCTGAAGGCG
    GCTCTCTCTG AGGAGGAGTT AGAGAAGAAA TCCAAGGCTA TCATTGAGGA ATATCTCCAT
    CTCAATGACA TGAAAGAGGC AGTCCAGTGC GTGCAGGAGC TGGCCTCACC CTCCTTGCTC
    TTCATCTTTG TACGGCATGG TGTCGAGTCT ACGCTGGAGC GCAGTGCCAT TGCTCGTGAG
    CATATGGGGC AGCTGCTGCA CCAGCTGCTC TGTGCTGGGC ATCTGTCTAC TGCTCAGTAC
    TACCAAGGGT TGTATGAAAT CTTGGAATTG GCTGAGGACA TGGAAATTGA CATCCCCCAC
    GTGTGGCTCT ACCTAGCGGA ACTGGTAACA CCCATTCTGC AGGAAGGTGG GGTGCCCATG
    GGGGAGCTGT TCAGGGAGAT TACAAAGCCT CTGAGACCGT TGGGCAAAGC TGCTTCCCTG
    TTGCTGGAGA TCCTGGGCCT CCTGTGCAAA AGCATGGGTC CTAAAAAGGT GGGGACGCTG
    TGGCGAGAAG CCGGGCTTAG CTGGAAGGAA TTTCTACCTG AAGGCCAGGA CATTGGTGCA
    TTCGTCGCTG AACAGAAGGT GGAGTATACC CTGGGAGAGG AGTCGGAAGC CCCTGGCCAG
    AGGGCACTCC CCTCCGAGGA GCTGAACAGG CAGCTGGAGA AGCTGCTGAA GGAGGGCAGC
    AGTAACCAGC GGGTGTTCGA CTGGATAGAG GCCAACCTGA GTGAGCAGCA GATAGTATCC
    AACACGTTAG TTCGAGCCCT CATGACGGCT GTCTGCTATT CTGCAATTAT TTTTGAGACT
    CCCCTCCGAG TGGACGTTGC AGTGCTGAAA GCGCGAGCGA AGCTGCTGCA GAAATACCTG
    TGTGACGAGC AGAAGGAGCT ACAGGCGCTC TACGCCCTCC AGGCCCTTGT AGTGACCTTA
    GAACAGCCTC CCAACCTGCT GCGGATGTTC TTTGACGCAC TGTATGACGA GGACGTGGTG
    AAGGAGGATG CCTTCTACAG TTGGGAGAGT AGCAAGGACC CCGCTGAGCA GCAGGGCAAG
    GGTGTGGCCC TTAAATCTGT CACAGCCTTC TTCAAGTGGC TCCGTGAAGC AGAGGAGGAG
    TCTGACCACA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13776
    Clone ID Related Accession (Same CDS sequence) NM_004953.4 , NM_004953.5
    Accession Version NM_004953.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4215bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-29
    Organism Homo sapiens(human)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20 and BQ011010.1. On Jun 2, 2019 this sequence version replaced NM_004953.4. Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream start codon, compared to variant 1. The resulting isoform (4) is shorter at the N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D12686.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCTGGGG CCCGCACTGC CTCCACACCC ACCCCTCCCC AGACGGGAGG CGGTCTGGAG 
    CCTCAAGCTA ATGGGGAGAC GCCCCAGGTT GCTGTCATTG TCCGGCCAGA TGACCGGTCA
    CAGGGAGCAA TCATTGCTGA CCGGCCAGGG CTGCCTGGCC CAGAGCATAG CCCTTCAGAA
    TCCCAGCCTT CGTCGCCTTC TCCGACCCCA TCACCATCCC CAGTCTTGGA ACCGGGGTCT
    GAGCCTAATC TCGCAGTCCT CTCTATTCCT GGGGACACTA TGACAACTAT ACAAATGTCT
    GTAGAAGAAT CAACCCCCAT CTCCCGTGAA ACTGGGGAGC CATATCGCCT CTCTCCAGAA
    CCCACTCCTC TCGCCGAACC CATACTGGAA GTAGAAGTGA CACTTAGCAA ACCGGTTCCA
    GAATCTGAGT TTTCTTCCAG TCCTCTCCAG GCTCCCACCC CTTTGGCATC TCACACAGTG
    GAAATTCATG AGCCTAATGG CATGGTCCCA TCTGAAGATC TGGAACCAGA GGTGGAGTCA
    AGCCCAGAGC TTGCTCCTCC CCCAGCTTGC CCCTCCGAAT CCCCTGTGCC CATTGCTCCA
    ACTGCCCAAC CTGAGGAACT GCTCAACGGA GCCCCCTCGC CACCAGCTGT GGACTTAAGC
    CCAGTCAGTG AGCCAGAGGA GCAGGCCAAG GAGGTGACAG CATCAATGGC GCCCCCCACC
    ATCCCCTCTG CTACTCCAGC TACGGCTCCT TCAGCTACTT CCCCAGCTCA GGAGGAGGAA
    ATGGAAGAAG AAGAAGAAGA GGAAGAAGGA GAAGCAGGAG AAGCAGGAGA AGCTGAGAGT
    GAGAAAGGAG GAGAGGAACT GCTCCCCCCA GAGAGTACCC CTATTCCAGC CAACTTGTCT
    CAGAATTTGG AGGCAGCAGC AGCCACTCAA GTGGCAGTAT CTGTGCCAAA GAGGAGACGG
    AAAATTAAGG AGCTAAATAA GAAGGAGGCT GTTGGAGACC TTCTGGATGC CTTCAAGGAG
    GCGAACCCGG CAGTACCAGA GGTGGAAAAT CAGCCTCCTG CAGGCAGCAA TCCAGGCCCA
    GAGTCTGAGG GCAGTGGTGT GCCCCCACGT CCTGAGGAAG CAGATGAGAC CTGGGACTCA
    AAGGAAGACA AAATTCACAA TGCTGAGAAC ATCCAGCCCG GGGAACAGAA GTATGAATAT
    AAGTCAGATC AGTGGAAGCC TCTAAACCTA GAGGAGAAAA AACGTTACGA CCGTGAGTTC
    CTGCTTGGTT TTCAGTTCAT CTTTGCCAGT ATGCAGAAGC CAGAGGGATT GCCACATATC
    AGTGACGTGG TGCTGGACAA GGCCAATAAA ACACCACTGC GGCCACTGGA TCCCACTAGA
    CTACAAGGCA TAAATTGTGG CCCAGACTTC ACTCCATCCT TTGCCAACCT TGGCCGGACA
    ACCCTTAGCA CCCGTGGGCC CCCAAGGGGT GGGCCAGGTG GGGAGCTGCC CCGTGGGCCG
    CAGGCTGGCC TGGGACCCCG GCGCTCTCAG CAGGGACCCC GAAAAGAACC ACGCAAGATC
    ATTGCCACAG TGTTAATGAC CGAAGATATA AAACTGAACA AAGCAGAGAA AGCCTGGAAA
    CCCAGCAGCA AGCGGACGGC GGCTGATAAG GATCGAGGGG AAGAAGATGC TGATGGCAGC
    AAAACCCAGG ACCTATTCCG CAGGGTGCGC TCCATCCTGA ATAAACTGAC ACCCCAGATG
    TTCCAGCAGC TGATGAAGCA AGTGACGCAG CTGGCCATCG ACACCGAGGA ACGCCTCAAA
    GGGGTCATTG ACCTCATTTT TGAGAAGGCC ATTTCAGAGC CCAACTTCTC TGTGGCCTAT
    GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACGGAAAA GCCAACAGTG
    ACTGTGAACT TCCGAAAGCT GTTGTTGAAT CGATGTCAGA AGGAGTTTGA GAAAGACAAA
    GATGATGATG AGGTTTTTGA GAAGAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAG
    GAACGAGGAC GCCTGAAGGA AGAGCTGGAA GAGGCTCGGG ACATAGCCCG GCGGCGCTCT
    TTAGGGAATA TCAAGTTTAT TGGAGAGTTG TTCAAACTGA AGATGTTAAC AGAGGCAATA
    ATGCATGACT GTGTGGTCAA ACTGCTTAAG AACCATGATG AAGAGTCCCT TGAGTGCCTT
    TGTCGTCTGC TCACCACCAT TGGCAAAGAC CTGGACTTTG AAAAAGCCAA GCCCCGAATG
    GATCAGTATT TCAACCAGAT GGAAAAAATC ATTAAAGAAA AGAAGACGTC ATCCCGCATC
    CGCTTTATGC TGCAGGACGT GCTGGATCTG CGAGGGAGCA ATTGGGTGCC ACGCCGAGGG
    GATCAGGGTC CCAAGACCAT TGACCAGATC CATAAGGAGG CTGAGATGGA AGAACATCGA
    GAGCACATCA AAGTGCAGCA GCTCATGGCC AAGGGCAGTG ACAAGCGTCG GGGCGGTCCT
    CCAGGCCCTC CCATCAGCCG TGGACTTCCC CTTGTGGATG ATGGTGGCTG GAACACAGTT
    CCCATCAGCA AAGGTAGCCG CCCCATTGAC ACCTCACGAC TCACCAAGAT CACCAAGCCT
    GGCTCCATCG ATTCTAACAA CCAGCTCTTT GCACCTGGAG GGCGACTGAG CTGGGGCAAG
    GGCAGCAGCG GAGGCTCAGG AGCCAAGCCC TCAGACGCAG CATCAGAAGC TGCTCGCCCA
    GCTACTAGTA CTTTGAATCG CTTCTCAGCC CTTCAACAAG CGGTACCCAC AGAAAGCACA
    GATAATAGAC GTGTGGTGCA GAGGAGTAGC TTGAGCCGAG AACGAGGCGA GAAAGCTGGA
    GACCGAGGAG ACCGCCTAGA GCGGAGTGAA CGGGGAGGGG ACCGTGGGGA CCGGCTTGAT
    CGTGCGCGGA CACCTGCTAC CAAGCGGAGC TTCAGCAAGG AAGTGGAGGA GCGGAGTAGA
    GAACGGCCCT CCCAGCCTGA GGGGCTGCGC AAGGCAGCTA GCCTCACGGA GGATCGGGAC
    CGTGGGCGGG ATGCCGTGAA GCGAGAAGCT GCCCTACCCC CAGTGAGCCC CCTGAAGGCG
    GCTCTCTCTG AGGAGGAGTT AGAGAAGAAA TCCAAGGCTA TCATTGAGGA ATATCTCCAT
    CTCAATGACA TGAAAGAGGC AGTCCAGTGC GTGCAGGAGC TGGCCTCACC CTCCTTGCTC
    TTCATCTTTG TACGGCATGG TGTCGAGTCT ACGCTGGAGC GCAGTGCCAT TGCTCGTGAG
    CATATGGGGC AGCTGCTGCA CCAGCTGCTC TGTGCTGGGC ATCTGTCTAC TGCTCAGTAC
    TACCAAGGGT TGTATGAAAT CTTGGAATTG GCTGAGGACA TGGAAATTGA CATCCCCCAC
    GTGTGGCTCT ACCTAGCGGA ACTGGTAACA CCCATTCTGC AGGAAGGTGG GGTGCCCATG
    GGGGAGCTGT TCAGGGAGAT TACAAAGCCT CTGAGACCGT TGGGCAAAGC TGCTTCCCTG
    TTGCTGGAGA TCCTGGGCCT CCTGTGCAAA AGCATGGGTC CTAAAAAGGT GGGGACGCTG
    TGGCGAGAAG CCGGGCTTAG CTGGAAGGAA TTTCTACCTG AAGGCCAGGA CATTGGTGCA
    TTCGTCGCTG AACAGAAGGT GGAGTATACC CTGGGAGAGG AGTCGGAAGC CCCTGGCCAG
    AGGGCACTCC CCTCCGAGGA GCTGAACAGG CAGCTGGAGA AGCTGCTGAA GGAGGGCAGC
    AGTAACCAGC GGGTGTTCGA CTGGATAGAG GCCAACCTGA GTGAGCAGCA GATAGTATCC
    AACACGTTAG TTCGAGCCCT CATGACGGCT GTCTGCTATT CTGCAATTAT TTTTGAGACT
    CCCCTCCGAG TGGACGTTGC AGTGCTGAAA GCGCGAGCGA AGCTGCTGCA GAAATACCTG
    TGTGACGAGC AGAAGGAGCT ACAGGCGCTC TACGCCCTCC AGGCCCTTGT AGTGACCTTA
    GAACAGCCTC CCAACCTGCT GCGGATGTTC TTTGACGCAC TGTATGACGA GGACGTGGTG
    AAGGAGGATG CCTTCTACAG TTGGGAGAGT AGCAAGGACC CCGCTGAGCA GCAGGGCAAG
    GGTGTGGCCC TTAAATCTGT CACAGCCTTC TTCAAGTGGC TCCGTGAAGC AGAGGAGGAG
    TCTGACCACA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004944.3
    CDS419..4633
    Translation

    Target ORF information:

    RefSeq Version NM_004953.5
    Organism Homo sapiens(human)
    Definition Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004953.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCTGGGG CCCGCACTGC CTCCACACCC ACCCCTCCCC AGACGGGAGG CGGTCTGGAG 
    CCTCAAGCTA ATGGGGAGAC GCCCCAGGTT GCTGTCATTG TCCGGCCAGA TGACCGGTCA
    CAGGGAGCAA TCATTGCTGA CCGGCCAGGG CTGCCTGGCC CAGAGCATAG CCCTTCAGAA
    TCCCAGCCTT CGTCGCCTTC TCCGACCCCA TCACCATCCC CAGTCTTGGA ACCGGGGTCT
    GAGCCTAATC TCGCAGTCCT CTCTATTCCT GGGGACACTA TGACAACTAT ACAAATGTCT
    GTAGAAGAAT CAACCCCCAT CTCCCGTGAA ACTGGGGAGC CATATCGCCT CTCTCCAGAA
    CCCACTCCTC TCGCCGAACC CATACTGGAA GTAGAAGTGA CACTTAGCAA ACCGGTTCCA
    GAATCTGAGT TTTCTTCCAG TCCTCTCCAG GCTCCCACCC CTTTGGCATC TCACACAGTG
    GAAATTCATG AGCCTAATGG CATGGTCCCA TCTGAAGATC TGGAACCAGA GGTGGAGTCA
    AGCCCAGAGC TTGCTCCTCC CCCAGCTTGC CCCTCCGAAT CCCCTGTGCC CATTGCTCCA
    ACTGCCCAAC CTGAGGAACT GCTCAACGGA GCCCCCTCGC CACCAGCTGT GGACTTAAGC
    CCAGTCAGTG AGCCAGAGGA GCAGGCCAAG GAGGTGACAG CATCAATGGC GCCCCCCACC
    ATCCCCTCTG CTACTCCAGC TACGGCTCCT TCAGCTACTT CCCCAGCTCA GGAGGAGGAA
    ATGGAAGAAG AAGAAGAAGA GGAAGAAGGA GAAGCAGGAG AAGCAGGAGA AGCTGAGAGT
    GAGAAAGGAG GAGAGGAACT GCTCCCCCCA GAGAGTACCC CTATTCCAGC CAACTTGTCT
    CAGAATTTGG AGGCAGCAGC AGCCACTCAA GTGGCAGTAT CTGTGCCAAA GAGGAGACGG
    AAAATTAAGG AGCTAAATAA GAAGGAGGCT GTTGGAGACC TTCTGGATGC CTTCAAGGAG
    GCGAACCCGG CAGTACCAGA GGTGGAAAAT CAGCCTCCTG CAGGCAGCAA TCCAGGCCCA
    GAGTCTGAGG GCAGTGGTGT GCCCCCACGT CCTGAGGAAG CAGATGAGAC CTGGGACTCA
    AAGGAAGACA AAATTCACAA TGCTGAGAAC ATCCAGCCCG GGGAACAGAA GTATGAATAT
    AAGTCAGATC AGTGGAAGCC TCTAAACCTA GAGGAGAAAA AACGTTACGA CCGTGAGTTC
    CTGCTTGGTT TTCAGTTCAT CTTTGCCAGT ATGCAGAAGC CAGAGGGATT GCCACATATC
    AGTGACGTGG TGCTGGACAA GGCCAATAAA ACACCACTGC GGCCACTGGA TCCCACTAGA
    CTACAAGGCA TAAATTGTGG CCCAGACTTC ACTCCATCCT TTGCCAACCT TGGCCGGACA
    ACCCTTAGCA CCCGTGGGCC CCCAAGGGGT GGGCCAGGTG GGGAGCTGCC CCGTGGGCCG
    CAGGCTGGCC TGGGACCCCG GCGCTCTCAG CAGGGACCCC GAAAAGAACC ACGCAAGATC
    ATTGCCACAG TGTTAATGAC CGAAGATATA AAACTGAACA AAGCAGAGAA AGCCTGGAAA
    CCCAGCAGCA AGCGGACGGC GGCTGATAAG GATCGAGGGG AAGAAGATGC TGATGGCAGC
    AAAACCCAGG ACCTATTCCG CAGGGTGCGC TCCATCCTGA ATAAACTGAC ACCCCAGATG
    TTCCAGCAGC TGATGAAGCA AGTGACGCAG CTGGCCATCG ACACCGAGGA ACGCCTCAAA
    GGGGTCATTG ACCTCATTTT TGAGAAGGCC ATTTCAGAGC CCAACTTCTC TGTGGCCTAT
    GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACGGAAAA GCCAACAGTG
    ACTGTGAACT TCCGAAAGCT GTTGTTGAAT CGATGTCAGA AGGAGTTTGA GAAAGACAAA
    GATGATGATG AGGTTTTTGA GAAGAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAG
    GAACGAGGAC GCCTGAAGGA AGAGCTGGAA GAGGCTCGGG ACATAGCCCG GCGGCGCTCT
    TTAGGGAATA TCAAGTTTAT TGGAGAGTTG TTCAAACTGA AGATGTTAAC AGAGGCAATA
    ATGCATGACT GTGTGGTCAA ACTGCTTAAG AACCATGATG AAGAGTCCCT TGAGTGCCTT
    TGTCGTCTGC TCACCACCAT TGGCAAAGAC CTGGACTTTG AAAAAGCCAA GCCCCGAATG
    GATCAGTATT TCAACCAGAT GGAAAAAATC ATTAAAGAAA AGAAGACGTC ATCCCGCATC
    CGCTTTATGC TGCAGGACGT GCTGGATCTG CGAGGGAGCA ATTGGGTGCC ACGCCGAGGG
    GATCAGGGTC CCAAGACCAT TGACCAGATC CATAAGGAGG CTGAGATGGA AGAACATCGA
    GAGCACATCA AAGTGCAGCA GCTCATGGCC AAGGGCAGTG ACAAGCGTCG GGGCGGTCCT
    CCAGGCCCTC CCATCAGCCG TGGACTTCCC CTTGTGGATG ATGGTGGCTG GAACACAGTT
    CCCATCAGCA AAGGTAGCCG CCCCATTGAC ACCTCACGAC TCACCAAGAT CACCAAGCCT
    GGCTCCATCG ATTCTAACAA CCAGCTCTTT GCACCTGGAG GGCGACTGAG CTGGGGCAAG
    GGCAGCAGCG GAGGCTCAGG AGCCAAGCCC TCAGACGCAG CATCAGAAGC TGCTCGCCCA
    GCTACTAGTA CTTTGAATCG CTTCTCAGCC CTTCAACAAG CGGTACCCAC AGAAAGCACA
    GATAATAGAC GTGTGGTGCA GAGGAGTAGC TTGAGCCGAG AACGAGGCGA GAAAGCTGGA
    GACCGAGGAG ACCGCCTAGA GCGGAGTGAA CGGGGAGGGG ACCGTGGGGA CCGGCTTGAT
    CGTGCGCGGA CACCTGCTAC CAAGCGGAGC TTCAGCAAGG AAGTGGAGGA GCGGAGTAGA
    GAACGGCCCT CCCAGCCTGA GGGGCTGCGC AAGGCAGCTA GCCTCACGGA GGATCGGGAC
    CGTGGGCGGG ATGCCGTGAA GCGAGAAGCT GCCCTACCCC CAGTGAGCCC CCTGAAGGCG
    GCTCTCTCTG AGGAGGAGTT AGAGAAGAAA TCCAAGGCTA TCATTGAGGA ATATCTCCAT
    CTCAATGACA TGAAAGAGGC AGTCCAGTGC GTGCAGGAGC TGGCCTCACC CTCCTTGCTC
    TTCATCTTTG TACGGCATGG TGTCGAGTCT ACGCTGGAGC GCAGTGCCAT TGCTCGTGAG
    CATATGGGGC AGCTGCTGCA CCAGCTGCTC TGTGCTGGGC ATCTGTCTAC TGCTCAGTAC
    TACCAAGGGT TGTATGAAAT CTTGGAATTG GCTGAGGACA TGGAAATTGA CATCCCCCAC
    GTGTGGCTCT ACCTAGCGGA ACTGGTAACA CCCATTCTGC AGGAAGGTGG GGTGCCCATG
    GGGGAGCTGT TCAGGGAGAT TACAAAGCCT CTGAGACCGT TGGGCAAAGC TGCTTCCCTG
    TTGCTGGAGA TCCTGGGCCT CCTGTGCAAA AGCATGGGTC CTAAAAAGGT GGGGACGCTG
    TGGCGAGAAG CCGGGCTTAG CTGGAAGGAA TTTCTACCTG AAGGCCAGGA CATTGGTGCA
    TTCGTCGCTG AACAGAAGGT GGAGTATACC CTGGGAGAGG AGTCGGAAGC CCCTGGCCAG
    AGGGCACTCC CCTCCGAGGA GCTGAACAGG CAGCTGGAGA AGCTGCTGAA GGAGGGCAGC
    AGTAACCAGC GGGTGTTCGA CTGGATAGAG GCCAACCTGA GTGAGCAGCA GATAGTATCC
    AACACGTTAG TTCGAGCCCT CATGACGGCT GTCTGCTATT CTGCAATTAT TTTTGAGACT
    CCCCTCCGAG TGGACGTTGC AGTGCTGAAA GCGCGAGCGA AGCTGCTGCA GAAATACCTG
    TGTGACGAGC AGAAGGAGCT ACAGGCGCTC TACGCCCTCC AGGCCCTTGT AGTGACCTTA
    GAACAGCCTC CCAACCTGCT GCGGATGTTC TTTGACGCAC TGTATGACGA GGACGTGGTG
    AAGGAGGATG CCTTCTACAG TTGGGAGAGT AGCAAGGACC CCGCTGAGCA GCAGGGCAAG
    GGTGTGGCCC TTAAATCTGT CACAGCCTTC TTCAAGTGGC TCCGTGAAGC AGAGGAGGAG
    TCTGACCACA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18102
    Clone ID Related Accession (Same CDS sequence) NM_198241.2
    Accession Version NM_198241.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR980540.1, BX647812.1, BC007788.2 and BQ011010.1. This sequence is a reference standard in the RefSeqGene project. On Aug 14, 2010 this sequence version replaced NM_198241.1. Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (5) that is 1 aa shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX647812.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAAAG CTCCACAGTC CACAGGCCCC CCACCCGCCC CATCCCCCGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCGCCG GTGGTGTTCA GTACGCCACA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG
    GGGGCCTACT ACATCCCTGG ACAGGGGCGT TCCACATACG TTGTCCCGAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACAGA ATTTGGGACC
    TACGCTGGCG CCTACTATCC AGCCCAAGGG GTGCAGCAGT TTCCCACTGG CGTGGCCCCC
    GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG TAAGACGATC
    CGAATTCGAG ATCCAAACCA AGGAGGAAAG GATATCACAG AGGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC CCCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAAGCTAAT
    GGGGAGACGC CCCAGGTTGC TGTCATTGTC CGGCCAGATG ACCGGTCACA GGGAGCAATC
    ATTGCTGACC GGCCAGGGCT GCCTGGCCCA GAGCATAGCC CTTCAGAATC CCAGCCTTCG
    TCGCCTTCTC CGACCCCATC ACCATCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC
    GCAGTCCTCT CTATTCCTGG GGACACTATG ACAACTATAC AAATGTCTGT AGAAGAATCA
    ACCCCCATCT CCCGTGAAAC TGGGGAGCCA TATCGCCTCT CTCCAGAACC CACTCCTCTC
    GCCGAACCCA TACTGGAAGT AGAAGTGACA CTTAGCAAAC CGGTTCCAGA ATCTGAGTTT
    TCTTCCAGTC CTCTCCAGGC TCCCACCCCT TTGGCATCTC ACACAGTGGA AATTCATGAG
    CCTAATGGCA TGGTCCCATC TGAAGATCTG GAACCAGAGG TGGAGTCAAG CCCAGAGCTT
    GCTCCTCCCC CAGCTTGCCC CTCCGAATCC CCTGTGCCCA TTGCTCCAAC TGCCCAACCT
    GAGGAACTGC TCAACGGAGC CCCCTCGCCA CCAGCTGTGG ACTTAAGCCC AGTCAGTGAG
    CCAGAGGAGC AGGCCAAGGA GGTGACAGCA TCAATGGCGC CCCCCACCAT CCCCTCTGCT
    ACTCCAGCTA CGGCTCCTTC AGCTACTTCC CCAGCTCAGG AGGAGGAAAT GGAAGAAGAA
    GAAGAAGAGG AAGAAGGAGA AGCAGGAGAA GCAGGAGAAG CTGAGAGTGA GAAAGGAGGA
    GAGGAACTGC TCCCCCCAGA GAGTACCCCT ATTCCAGCCA ACTTGTCTCA GAATTTGGAG
    GCAGCAGCAG CCACTCAAGT GGCAGTATCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG
    CTAAATAAGA AGGAGGCTGT TGGAGACCTT CTGGATGCCT TCAAGGAGGC GAACCCGGCA
    GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCAGCAATC CAGGCCCAGA GTCTGAGGGC
    AGTGGTGTGC CCCCACGTCC TGAGGAAGCA GATGAGACCT GGGACTCAAA GGAAGACAAA
    ATTCACAATG CTGAGAACAT CCAGCCCGGG GAACAGAAGT ATGAATATAA GTCAGATCAG
    TGGAAGCCTC TAAACCTAGA GGAGAAAAAA CGTTACGACC GTGAGTTCCT GCTTGGTTTT
    CAGTTCATCT TTGCCAGTAT GCAGAAGCCA GAGGGATTGC CACATATCAG TGACGTGGTG
    CTGGACAAGG CCAATAAAAC ACCACTGCGG CCACTGGATC CCACTAGACT ACAAGGCATA
    AATTGTGGCC CAGACTTCAC TCCATCCTTT GCCAACCTTG GCCGGACAAC CCTTAGCACC
    CGTGGGCCCC CAAGGGGTGG GCCAGGTGGG GAGCTGCCCC GTGGGCCGGC TGGCCTGGGA
    CCCCGGCGCT CTCAGCAGGG ACCCCGAAAA GAACCACGCA AGATCATTGC CACAGTGTTA
    ATGACCGAAG ATATAAAACT GAACAAAGCA GAGAAAGCCT GGAAACCCAG CAGCAAGCGG
    ACGGCGGCTG ATAAGGATCG AGGGGAAGAA GATGCTGATG GCAGCAAAAC CCAGGACCTA
    TTCCGCAGGG TGCGCTCCAT CCTGAATAAA CTGACACCCC AGATGTTCCA GCAGCTGATG
    AAGCAAGTGA CGCAGCTGGC CATCGACACC GAGGAACGCC TCAAAGGGGT CATTGACCTC
    ATTTTTGAGA AGGCCATTTC AGAGCCCAAC TTCTCTGTGG CCTATGCCAA CATGTGCCGC
    TGCCTCATGG CGCTGAAAGT GCCCACTACG GAAAAGCCAA CAGTGACTGT GAACTTCCGA
    AAGCTGTTGT TGAATCGATG TCAGAAGGAG TTTGAGAAAG ACAAAGATGA TGATGAGGTT
    TTTGAGAAGA AGCAAAAAGA GATGGATGAA GCTGCTACGG CAGAGGAACG AGGACGCCTG
    AAGGAAGAGC TGGAAGAGGC TCGGGACATA GCCCGGCGGC GCTCTTTAGG GAATATCAAG
    TTTATTGGAG AGTTGTTCAA ACTGAAGATG TTAACAGAGG CAATAATGCA TGACTGTGTG
    GTCAAACTGC TTAAGAACCA TGATGAAGAG TCCCTTGAGT GCCTTTGTCG TCTGCTCACC
    ACCATTGGCA AAGACCTGGA CTTTGAAAAA GCCAAGCCCC GAATGGATCA GTATTTCAAC
    CAGATGGAAA AAATCATTAA AGAAAAGAAG ACGTCATCCC GCATCCGCTT TATGCTGCAG
    GACGTGCTGG ATCTGCGAGG GAGCAATTGG GTGCCACGCC GAGGGGATCA GGGTCCCAAG
    ACCATTGACC AGATCCATAA GGAGGCTGAG ATGGAAGAAC ATCGAGAGCA CATCAAAGTG
    CAGCAGCTCA TGGCCAAGGG CAGTGACAAG CGTCGGGGCG GTCCTCCAGG CCCTCCCATC
    AGCCGTGGAC TTCCCCTTGT GGATGATGGT GGCTGGAACA CAGTTCCCAT CAGCAAAGGT
    AGCCGCCCCA TTGACACCTC ACGACTCACC AAGATCACCA AGCCTGGCTC CATCGATTCT
    AACAACCAGC TCTTTGCACC TGGAGGGCGA CTGAGCTGGG GCAAGGGCAG CAGCGGAGGC
    TCAGGAGCCA AGCCCTCAGA CGCAGCATCA GAAGCTGCTC GCCCAGCTAC TAGTACTTTG
    AATCGCTTCT CAGCCCTTCA ACAAGCGGTA CCCACAGAAA GCACAGATAA TAGACGTGTG
    GTGCAGAGGA GTAGCTTGAG CCGAGAACGA GGCGAGAAAG CTGGAGACCG AGGAGACCGC
    CTAGAGCGGA GTGAACGGGG AGGGGACCGT GGGGACCGGC TTGATCGTGC GCGGACACCT
    GCTACCAAGC GGAGCTTCAG CAAGGAAGTG GAGGAGCGGA GTAGAGAACG GCCCTCCCAG
    CCTGAGGGGC TGCGCAAGGC AGCTAGCCTC ACGGAGGATC GGGACCGTGG GCGGGATGCC
    GTGAAGCGAG AAGCTGCCCT ACCCCCAGTG AGCCCCCTGA AGGCGGCTCT CTCTGAGGAG
    GAGTTAGAGA AGAAATCCAA GGCTATCATT GAGGAATATC TCCATCTCAA TGACATGAAA
    GAGGCAGTCC AGTGCGTGCA GGAGCTGGCC TCACCCTCCT TGCTCTTCAT CTTTGTACGG
    CATGGTGTCG AGTCTACGCT GGAGCGCAGT GCCATTGCTC GTGAGCATAT GGGGCAGCTG
    CTGCACCAGC TGCTCTGTGC TGGGCATCTG TCTACTGCTC AGTACTACCA AGGGTTGTAT
    GAAATCTTGG AATTGGCTGA GGACATGGAA ATTGACATCC CCCACGTGTG GCTCTACCTA
    GCGGAACTGG TAACACCCAT TCTGCAGGAA GGTGGGGTGC CCATGGGGGA GCTGTTCAGG
    GAGATTACAA AGCCTCTGAG ACCGTTGGGC AAAGCTGCTT CCCTGTTGCT GGAGATCCTG
    GGCCTCCTGT GCAAAAGCAT GGGTCCTAAA AAGGTGGGGA CGCTGTGGCG AGAAGCCGGG
    CTTAGCTGGA AGGAATTTCT ACCTGAAGGC CAGGACATTG GTGCATTCGT CGCTGAACAG
    AAGGTGGAGT ATACCCTGGG AGAGGAGTCG GAAGCCCCTG GCCAGAGGGC ACTCCCCTCC
    GAGGAGCTGA ACAGGCAGCT GGAGAAGCTG CTGAAGGAGG GCAGCAGTAA CCAGCGGGTG
    TTCGACTGGA TAGAGGCCAA CCTGAGTGAG CAGCAGATAG TATCCAACAC GTTAGTTCGA
    GCCCTCATGA CGGCTGTCTG CTATTCTGCA ATTATTTTTG AGACTCCCCT CCGAGTGGAC
    GTTGCAGTGC TGAAAGCGCG AGCGAAGCTG CTGCAGAAAT ACCTGTGTGA CGAGCAGAAG
    GAGCTACAGG CGCTCTACGC CCTCCAGGCC CTTGTAGTGA CCTTAGAACA GCCTCCCAAC
    CTGCTGCGGA TGTTCTTTGA CGCACTGTAT GACGAGGACG TGGTGAAGGA GGATGCCTTC
    TACAGTTGGG AGAGTAGCAA GGACCCCGCT GAGCAGCAGG GCAAGGGTGT GGCCCTTAAA
    TCTGTCACAG CCTTCTTCAA GTGGCTCCGT GAAGCAGAGG AGGAGTCTGA CCACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_937884.1
    CDS272..5071
    Translation

    Target ORF information:

    RefSeq Version NM_198241.2
    Organism Homo sapiens(human)
    Definition Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_198241.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAAAG CTCCACAGTC CACAGGCCCC CCACCCGCCC CATCCCCCGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCGCCG GTGGTGTTCA GTACGCCACA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG
    GGGGCCTACT ACATCCCTGG ACAGGGGCGT TCCACATACG TTGTCCCGAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACAGA ATTTGGGACC
    TACGCTGGCG CCTACTATCC AGCCCAAGGG GTGCAGCAGT TTCCCACTGG CGTGGCCCCC
    GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG TAAGACGATC
    CGAATTCGAG ATCCAAACCA AGGAGGAAAG GATATCACAG AGGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC CCCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAAGCTAAT
    GGGGAGACGC CCCAGGTTGC TGTCATTGTC CGGCCAGATG ACCGGTCACA GGGAGCAATC
    ATTGCTGACC GGCCAGGGCT GCCTGGCCCA GAGCATAGCC CTTCAGAATC CCAGCCTTCG
    TCGCCTTCTC CGACCCCATC ACCATCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC
    GCAGTCCTCT CTATTCCTGG GGACACTATG ACAACTATAC AAATGTCTGT AGAAGAATCA
    ACCCCCATCT CCCGTGAAAC TGGGGAGCCA TATCGCCTCT CTCCAGAACC CACTCCTCTC
    GCCGAACCCA TACTGGAAGT AGAAGTGACA CTTAGCAAAC CGGTTCCAGA ATCTGAGTTT
    TCTTCCAGTC CTCTCCAGGC TCCCACCCCT TTGGCATCTC ACACAGTGGA AATTCATGAG
    CCTAATGGCA TGGTCCCATC TGAAGATCTG GAACCAGAGG TGGAGTCAAG CCCAGAGCTT
    GCTCCTCCCC CAGCTTGCCC CTCCGAATCC CCTGTGCCCA TTGCTCCAAC TGCCCAACCT
    GAGGAACTGC TCAACGGAGC CCCCTCGCCA CCAGCTGTGG ACTTAAGCCC AGTCAGTGAG
    CCAGAGGAGC AGGCCAAGGA GGTGACAGCA TCAATGGCGC CCCCCACCAT CCCCTCTGCT
    ACTCCAGCTA CGGCTCCTTC AGCTACTTCC CCAGCTCAGG AGGAGGAAAT GGAAGAAGAA
    GAAGAAGAGG AAGAAGGAGA AGCAGGAGAA GCAGGAGAAG CTGAGAGTGA GAAAGGAGGA
    GAGGAACTGC TCCCCCCAGA GAGTACCCCT ATTCCAGCCA ACTTGTCTCA GAATTTGGAG
    GCAGCAGCAG CCACTCAAGT GGCAGTATCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG
    CTAAATAAGA AGGAGGCTGT TGGAGACCTT CTGGATGCCT TCAAGGAGGC GAACCCGGCA
    GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCAGCAATC CAGGCCCAGA GTCTGAGGGC
    AGTGGTGTGC CCCCACGTCC TGAGGAAGCA GATGAGACCT GGGACTCAAA GGAAGACAAA
    ATTCACAATG CTGAGAACAT CCAGCCCGGG GAACAGAAGT ATGAATATAA GTCAGATCAG
    TGGAAGCCTC TAAACCTAGA GGAGAAAAAA CGTTACGACC GTGAGTTCCT GCTTGGTTTT
    CAGTTCATCT TTGCCAGTAT GCAGAAGCCA GAGGGATTGC CACATATCAG TGACGTGGTG
    CTGGACAAGG CCAATAAAAC ACCACTGCGG CCACTGGATC CCACTAGACT ACAAGGCATA
    AATTGTGGCC CAGACTTCAC TCCATCCTTT GCCAACCTTG GCCGGACAAC CCTTAGCACC
    CGTGGGCCCC CAAGGGGTGG GCCAGGTGGG GAGCTGCCCC GTGGGCCGGC TGGCCTGGGA
    CCCCGGCGCT CTCAGCAGGG ACCCCGAAAA GAACCACGCA AGATCATTGC CACAGTGTTA
    ATGACCGAAG ATATAAAACT GAACAAAGCA GAGAAAGCCT GGAAACCCAG CAGCAAGCGG
    ACGGCGGCTG ATAAGGATCG AGGGGAAGAA GATGCTGATG GCAGCAAAAC CCAGGACCTA
    TTCCGCAGGG TGCGCTCCAT CCTGAATAAA CTGACACCCC AGATGTTCCA GCAGCTGATG
    AAGCAAGTGA CGCAGCTGGC CATCGACACC GAGGAACGCC TCAAAGGGGT CATTGACCTC
    ATTTTTGAGA AGGCCATTTC AGAGCCCAAC TTCTCTGTGG CCTATGCCAA CATGTGCCGC
    TGCCTCATGG CGCTGAAAGT GCCCACTACG GAAAAGCCAA CAGTGACTGT GAACTTCCGA
    AAGCTGTTGT TGAATCGATG TCAGAAGGAG TTTGAGAAAG ACAAAGATGA TGATGAGGTT
    TTTGAGAAGA AGCAAAAAGA GATGGATGAA GCTGCTACGG CAGAGGAACG AGGACGCCTG
    AAGGAAGAGC TGGAAGAGGC TCGGGACATA GCCCGGCGGC GCTCTTTAGG GAATATCAAG
    TTTATTGGAG AGTTGTTCAA ACTGAAGATG TTAACAGAGG CAATAATGCA TGACTGTGTG
    GTCAAACTGC TTAAGAACCA TGATGAAGAG TCCCTTGAGT GCCTTTGTCG TCTGCTCACC
    ACCATTGGCA AAGACCTGGA CTTTGAAAAA GCCAAGCCCC GAATGGATCA GTATTTCAAC
    CAGATGGAAA AAATCATTAA AGAAAAGAAG ACGTCATCCC GCATCCGCTT TATGCTGCAG
    GACGTGCTGG ATCTGCGAGG GAGCAATTGG GTGCCACGCC GAGGGGATCA GGGTCCCAAG
    ACCATTGACC AGATCCATAA GGAGGCTGAG ATGGAAGAAC ATCGAGAGCA CATCAAAGTG
    CAGCAGCTCA TGGCCAAGGG CAGTGACAAG CGTCGGGGCG GTCCTCCAGG CCCTCCCATC
    AGCCGTGGAC TTCCCCTTGT GGATGATGGT GGCTGGAACA CAGTTCCCAT CAGCAAAGGT
    AGCCGCCCCA TTGACACCTC ACGACTCACC AAGATCACCA AGCCTGGCTC CATCGATTCT
    AACAACCAGC TCTTTGCACC TGGAGGGCGA CTGAGCTGGG GCAAGGGCAG CAGCGGAGGC
    TCAGGAGCCA AGCCCTCAGA CGCAGCATCA GAAGCTGCTC GCCCAGCTAC TAGTACTTTG
    AATCGCTTCT CAGCCCTTCA ACAAGCGGTA CCCACAGAAA GCACAGATAA TAGACGTGTG
    GTGCAGAGGA GTAGCTTGAG CCGAGAACGA GGCGAGAAAG CTGGAGACCG AGGAGACCGC
    CTAGAGCGGA GTGAACGGGG AGGGGACCGT GGGGACCGGC TTGATCGTGC GCGGACACCT
    GCTACCAAGC GGAGCTTCAG CAAGGAAGTG GAGGAGCGGA GTAGAGAACG GCCCTCCCAG
    CCTGAGGGGC TGCGCAAGGC AGCTAGCCTC ACGGAGGATC GGGACCGTGG GCGGGATGCC
    GTGAAGCGAG AAGCTGCCCT ACCCCCAGTG AGCCCCCTGA AGGCGGCTCT CTCTGAGGAG
    GAGTTAGAGA AGAAATCCAA GGCTATCATT GAGGAATATC TCCATCTCAA TGACATGAAA
    GAGGCAGTCC AGTGCGTGCA GGAGCTGGCC TCACCCTCCT TGCTCTTCAT CTTTGTACGG
    CATGGTGTCG AGTCTACGCT GGAGCGCAGT GCCATTGCTC GTGAGCATAT GGGGCAGCTG
    CTGCACCAGC TGCTCTGTGC TGGGCATCTG TCTACTGCTC AGTACTACCA AGGGTTGTAT
    GAAATCTTGG AATTGGCTGA GGACATGGAA ATTGACATCC CCCACGTGTG GCTCTACCTA
    GCGGAACTGG TAACACCCAT TCTGCAGGAA GGTGGGGTGC CCATGGGGGA GCTGTTCAGG
    GAGATTACAA AGCCTCTGAG ACCGTTGGGC AAAGCTGCTT CCCTGTTGCT GGAGATCCTG
    GGCCTCCTGT GCAAAAGCAT GGGTCCTAAA AAGGTGGGGA CGCTGTGGCG AGAAGCCGGG
    CTTAGCTGGA AGGAATTTCT ACCTGAAGGC CAGGACATTG GTGCATTCGT CGCTGAACAG
    AAGGTGGAGT ATACCCTGGG AGAGGAGTCG GAAGCCCCTG GCCAGAGGGC ACTCCCCTCC
    GAGGAGCTGA ACAGGCAGCT GGAGAAGCTG CTGAAGGAGG GCAGCAGTAA CCAGCGGGTG
    TTCGACTGGA TAGAGGCCAA CCTGAGTGAG CAGCAGATAG TATCCAACAC GTTAGTTCGA
    GCCCTCATGA CGGCTGTCTG CTATTCTGCA ATTATTTTTG AGACTCCCCT CCGAGTGGAC
    GTTGCAGTGC TGAAAGCGCG AGCGAAGCTG CTGCAGAAAT ACCTGTGTGA CGAGCAGAAG
    GAGCTACAGG CGCTCTACGC CCTCCAGGCC CTTGTAGTGA CCTTAGAACA GCCTCCCAAC
    CTGCTGCGGA TGTTCTTTGA CGCACTGTAT GACGAGGACG TGGTGAAGGA GGATGCCTTC
    TACAGTTGGG AGAGTAGCAA GGACCCCGCT GAGCAGCAGG GCAAGGGTGT GGCCCTTAAA
    TCTGTCACAG CCTTCTTCAA GTGGCTCCGT GAAGCAGAGG AGGAGTCTGA CCACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108646
    Clone ID Related Accession (Same CDS sequence) NM_001194946.2
    Accession Version NM_001194946.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4821bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-20
    Organism Homo sapiens(human)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform 6
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20. On Jun 2, 2019 this sequence version replaced NM_001194946.1. Transcript Variant: This variant (6) differs in the 5' UTR, includes an additional in-frame exon in the 5' coding region, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (6) is longer than isoform 1. Both variants 6 and 7 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC140892.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGTC CACAGGCCCC CCACCCGCCC CATCCCCCGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCGCCG GTGGTGTTCA GTACGCCACA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC
    CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGGCG TTCCACATAC
    GTTGTCCCGA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA
    AGCCCTACAG AATTTGGGAC CTACGCTGGC GCCTACTATC CAGCCCAAGG GGTGCAGCAG
    TTTCCCACTG GCGTGGCCCC CACCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCC
    AAGAGGGAGC GTAAGACGAT CCGAATTCGA GATCCAAACC AAGGAGGAAA GGATATCACA
    GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CCCCTCCCCA GACGGGAGGC
    GGTCTGGAGC CTCAAGCTAA TGGGGAGACG CCCCAGGTTG CTGTCATTGT CCGGCCAGAT
    GACCGGTCAC AGGGAGCAAT CATTGCTGAC CGGCCAGGGC TGCCTGGCCC AGAGCATAGC
    CCTTCAGAAT CCCAGCCTTC GTCGCCTTCT CCGACCCCAT CACCATCCCC AGTCTTGGAA
    CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTATTCCTG GGGACACTAT GACAACTATA
    CAAATGTCTG TAGAAGAATC AACCCCCATC TCCCGTGAAA CTGGGGAGCC ATATCGCCTC
    TCTCCAGAAC CCACTCCTCT CGCCGAACCC ATACTGGAAG TAGAAGTGAC ACTTAGCAAA
    CCGGTTCCAG AATCTGAGTT TTCTTCCAGT CCTCTCCAGG CTCCCACCCC TTTGGCATCT
    CACACAGTGG AAATTCATGA GCCTAATGGC ATGGTCCCAT CTGAAGATCT GGAACCAGAG
    GTGGAGTCAA GCCCAGAGCT TGCTCCTCCC CCAGCTTGCC CCTCCGAATC CCCTGTGCCC
    ATTGCTCCAA CTGCCCAACC TGAGGAACTG CTCAACGGAG CCCCCTCGCC ACCAGCTGTG
    GACTTAAGCC CAGTCAGTGA GCCAGAGGAG CAGGCCAAGG AGGTGACAGC ATCAATGGCG
    CCCCCCACCA TCCCCTCTGC TACTCCAGCT ACGGCTCCTT CAGCTACTTC CCCAGCTCAG
    GAGGAGGAAA TGGAAGAAGA AGAAGAAGAG GAAGAAGGAG AAGCAGGAGA AGCAGGAGAA
    GCTGAGAGTG AGAAAGGAGG AGAGGAACTG CTCCCCCCAG AGAGTACCCC TATTCCAGCC
    AACTTGTCTC AGAATTTGGA GGCAGCAGCA GCCACTCAAG TGGCAGTATC TGTGCCAAAG
    AGGAGACGGA AAATTAAGGA GCTAAATAAG AAGGAGGCTG TTGGAGACCT TCTGGATGCC
    TTCAAGGAGG CGAACCCGGC AGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCAGCAAT
    CCAGGCCCAG AGTCTGAGGG CAGTGGTGTG CCCCCACGTC CTGAGGAAGC AGATGAGACC
    TGGGACTCAA AGGAAGACAA AATTCACAAT GCTGAGAACA TCCAGCCCGG GGAACAGAAG
    TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTAG AGGAGAAAAA ACGTTACGAC
    CGTGAGTTCC TGCTTGGTTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC AGAGGGATTG
    CCACATATCA GTGACGTGGT GCTGGACAAG GCCAATAAAA CACCACTGCG GCCACTGGAT
    CCCACTAGAC TACAAGGCAT AAATTGTGGC CCAGACTTCA CTCCATCCTT TGCCAACCTT
    GGCCGGACAA CCCTTAGCAC CCGTGGGCCC CCAAGGGGTG GGCCAGGTGG GGAGCTGCCC
    CGTGGGCCGG CTGGCCTGGG ACCCCGGCGC TCTCAGCAGG GACCCCGAAA AGAACCACGC
    AAGATCATTG CCACAGTGTT AATGACCGAA GATATAAAAC TGAACAAAGC AGAGAAAGCC
    TGGAAACCCA GCAGCAAGCG GACGGCGGCT GATAAGGATC GAGGGGAAGA AGATGCTGAT
    GGCAGCAAAA CCCAGGACCT ATTCCGCAGG GTGCGCTCCA TCCTGAATAA ACTGACACCC
    CAGATGTTCC AGCAGCTGAT GAAGCAAGTG ACGCAGCTGG CCATCGACAC CGAGGAACGC
    CTCAAAGGGG TCATTGACCT CATTTTTGAG AAGGCCATTT CAGAGCCCAA CTTCTCTGTG
    GCCTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC GGAAAAGCCA
    ACAGTGACTG TGAACTTCCG AAAGCTGTTG TTGAATCGAT GTCAGAAGGA GTTTGAGAAA
    GACAAAGATG ATGATGAGGT TTTTGAGAAG AAGCAAAAAG AGATGGATGA AGCTGCTACG
    GCAGAGGAAC GAGGACGCCT GAAGGAAGAG CTGGAAGAGG CTCGGGACAT AGCCCGGCGG
    CGCTCTTTAG GGAATATCAA GTTTATTGGA GAGTTGTTCA AACTGAAGAT GTTAACAGAG
    GCAATAATGC ATGACTGTGT GGTCAAACTG CTTAAGAACC ATGATGAAGA GTCCCTTGAG
    TGCCTTTGTC GTCTGCTCAC CACCATTGGC AAAGACCTGG ACTTTGAAAA AGCCAAGCCC
    CGAATGGATC AGTATTTCAA CCAGATGGAA AAAATCATTA AAGAAAAGAA GACGTCATCC
    CGCATCCGCT TTATGCTGCA GGACGTGCTG GATCTGCGAG GGAGCAATTG GGTGCCACGC
    CGAGGGGATC AGGGTCCCAA GACCATTGAC CAGATCCATA AGGAGGCTGA GATGGAAGAA
    CATCGAGAGC ACATCAAAGT GCAGCAGCTC ATGGCCAAGG GCAGTGACAA GCGTCGGGGC
    GGTCCTCCAG GCCCTCCCAT CAGCCGTGGA CTTCCCCTTG TGGATGATGG TGGCTGGAAC
    ACAGTTCCCA TCAGCAAAGG TAGCCGCCCC ATTGACACCT CACGACTCAC CAAGATCACC
    AAGCCTGGCT CCATCGATTC TAACAACCAG CTCTTTGCAC CTGGAGGGCG ACTGAGCTGG
    GGCAAGGGCA GCAGCGGAGG CTCAGGAGCC AAGCCCTCAG ACGCAGCATC AGAAGCTGCT
    CGCCCAGCTA CTAGTACTTT GAATCGCTTC TCAGCCCTTC AACAAGCGGT ACCCACAGAA
    AGCACAGATA ATAGACGTGT GGTGCAGAGG AGTAGCTTGA GCCGAGAACG AGGCGAGAAA
    GCTGGAGACC GAGGAGACCG CCTAGAGCGG AGTGAACGGG GAGGGGACCG TGGGGACCGG
    CTTGATCGTG CGCGGACACC TGCTACCAAG CGGAGCTTCA GCAAGGAAGT GGAGGAGCGG
    AGTAGAGAAC GGCCCTCCCA GCCTGAGGGG CTGCGCAAGG CAGCTAGCCT CACGGAGGAT
    CGGGACCGTG GGCGGGATGC CGTGAAGCGA GAAGCTGCCC TACCCCCAGT GAGCCCCCTG
    AAGGCGGCTC TCTCTGAGGA GGAGTTAGAG AAGAAATCCA AGGCTATCAT TGAGGAATAT
    CTCCATCTCA ATGACATGAA AGAGGCAGTC CAGTGCGTGC AGGAGCTGGC CTCACCCTCC
    TTGCTCTTCA TCTTTGTACG GCATGGTGTC GAGTCTACGC TGGAGCGCAG TGCCATTGCT
    CGTGAGCATA TGGGGCAGCT GCTGCACCAG CTGCTCTGTG CTGGGCATCT GTCTACTGCT
    CAGTACTACC AAGGGTTGTA TGAAATCTTG GAATTGGCTG AGGACATGGA AATTGACATC
    CCCCACGTGT GGCTCTACCT AGCGGAACTG GTAACACCCA TTCTGCAGGA AGGTGGGGTG
    CCCATGGGGG AGCTGTTCAG GGAGATTACA AAGCCTCTGA GACCGTTGGG CAAAGCTGCT
    TCCCTGTTGC TGGAGATCCT GGGCCTCCTG TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG
    ACGCTGTGGC GAGAAGCCGG GCTTAGCTGG AAGGAATTTC TACCTGAAGG CCAGGACATT
    GGTGCATTCG TCGCTGAACA GAAGGTGGAG TATACCCTGG GAGAGGAGTC GGAAGCCCCT
    GGCCAGAGGG CACTCCCCTC CGAGGAGCTG AACAGGCAGC TGGAGAAGCT GCTGAAGGAG
    GGCAGCAGTA ACCAGCGGGT GTTCGACTGG ATAGAGGCCA ACCTGAGTGA GCAGCAGATA
    GTATCCAACA CGTTAGTTCG AGCCCTCATG ACGGCTGTCT GCTATTCTGC AATTATTTTT
    GAGACTCCCC TCCGAGTGGA CGTTGCAGTG CTGAAAGCGC GAGCGAAGCT GCTGCAGAAA
    TACCTGTGTG ACGAGCAGAA GGAGCTACAG GCGCTCTACG CCCTCCAGGC CCTTGTAGTG
    ACCTTAGAAC AGCCTCCCAA CCTGCTGCGG ATGTTCTTTG ACGCACTGTA TGACGAGGAC
    GTGGTGAAGG AGGATGCCTT CTACAGTTGG GAGAGTAGCA AGGACCCCGC TGAGCAGCAG
    GGCAAGGGTG TGGCCCTTAA ATCTGTCACA GCCTTCTTCA AGTGGCTCCG TGAAGCAGAG
    GAGGAGTCTG ACCACAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001181875.2
    CDS161..4981
    Translation

    Target ORF information:

    RefSeq Version NM_001194946.2
    Organism Homo sapiens(human)
    Definition Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001194946.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGTC CACAGGCCCC CCACCCGCCC CATCCCCCGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCGCCG GTGGTGTTCA GTACGCCACA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC
    CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGGCG TTCCACATAC
    GTTGTCCCGA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA
    AGCCCTACAG AATTTGGGAC CTACGCTGGC GCCTACTATC CAGCCCAAGG GGTGCAGCAG
    TTTCCCACTG GCGTGGCCCC CACCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCC
    AAGAGGGAGC GTAAGACGAT CCGAATTCGA GATCCAAACC AAGGAGGAAA GGATATCACA
    GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CCCCTCCCCA GACGGGAGGC
    GGTCTGGAGC CTCAAGCTAA TGGGGAGACG CCCCAGGTTG CTGTCATTGT CCGGCCAGAT
    GACCGGTCAC AGGGAGCAAT CATTGCTGAC CGGCCAGGGC TGCCTGGCCC AGAGCATAGC
    CCTTCAGAAT CCCAGCCTTC GTCGCCTTCT CCGACCCCAT CACCATCCCC AGTCTTGGAA
    CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTATTCCTG GGGACACTAT GACAACTATA
    CAAATGTCTG TAGAAGAATC AACCCCCATC TCCCGTGAAA CTGGGGAGCC ATATCGCCTC
    TCTCCAGAAC CCACTCCTCT CGCCGAACCC ATACTGGAAG TAGAAGTGAC ACTTAGCAAA
    CCGGTTCCAG AATCTGAGTT TTCTTCCAGT CCTCTCCAGG CTCCCACCCC TTTGGCATCT
    CACACAGTGG AAATTCATGA GCCTAATGGC ATGGTCCCAT CTGAAGATCT GGAACCAGAG
    GTGGAGTCAA GCCCAGAGCT TGCTCCTCCC CCAGCTTGCC CCTCCGAATC CCCTGTGCCC
    ATTGCTCCAA CTGCCCAACC TGAGGAACTG CTCAACGGAG CCCCCTCGCC ACCAGCTGTG
    GACTTAAGCC CAGTCAGTGA GCCAGAGGAG CAGGCCAAGG AGGTGACAGC ATCAATGGCG
    CCCCCCACCA TCCCCTCTGC TACTCCAGCT ACGGCTCCTT CAGCTACTTC CCCAGCTCAG
    GAGGAGGAAA TGGAAGAAGA AGAAGAAGAG GAAGAAGGAG AAGCAGGAGA AGCAGGAGAA
    GCTGAGAGTG AGAAAGGAGG AGAGGAACTG CTCCCCCCAG AGAGTACCCC TATTCCAGCC
    AACTTGTCTC AGAATTTGGA GGCAGCAGCA GCCACTCAAG TGGCAGTATC TGTGCCAAAG
    AGGAGACGGA AAATTAAGGA GCTAAATAAG AAGGAGGCTG TTGGAGACCT TCTGGATGCC
    TTCAAGGAGG CGAACCCGGC AGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCAGCAAT
    CCAGGCCCAG AGTCTGAGGG CAGTGGTGTG CCCCCACGTC CTGAGGAAGC AGATGAGACC
    TGGGACTCAA AGGAAGACAA AATTCACAAT GCTGAGAACA TCCAGCCCGG GGAACAGAAG
    TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTAG AGGAGAAAAA ACGTTACGAC
    CGTGAGTTCC TGCTTGGTTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC AGAGGGATTG
    CCACATATCA GTGACGTGGT GCTGGACAAG GCCAATAAAA CACCACTGCG GCCACTGGAT
    CCCACTAGAC TACAAGGCAT AAATTGTGGC CCAGACTTCA CTCCATCCTT TGCCAACCTT
    GGCCGGACAA CCCTTAGCAC CCGTGGGCCC CCAAGGGGTG GGCCAGGTGG GGAGCTGCCC
    CGTGGGCCGG CTGGCCTGGG ACCCCGGCGC TCTCAGCAGG GACCCCGAAA AGAACCACGC
    AAGATCATTG CCACAGTGTT AATGACCGAA GATATAAAAC TGAACAAAGC AGAGAAAGCC
    TGGAAACCCA GCAGCAAGCG GACGGCGGCT GATAAGGATC GAGGGGAAGA AGATGCTGAT
    GGCAGCAAAA CCCAGGACCT ATTCCGCAGG GTGCGCTCCA TCCTGAATAA ACTGACACCC
    CAGATGTTCC AGCAGCTGAT GAAGCAAGTG ACGCAGCTGG CCATCGACAC CGAGGAACGC
    CTCAAAGGGG TCATTGACCT CATTTTTGAG AAGGCCATTT CAGAGCCCAA CTTCTCTGTG
    GCCTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC GGAAAAGCCA
    ACAGTGACTG TGAACTTCCG AAAGCTGTTG TTGAATCGAT GTCAGAAGGA GTTTGAGAAA
    GACAAAGATG ATGATGAGGT TTTTGAGAAG AAGCAAAAAG AGATGGATGA AGCTGCTACG
    GCAGAGGAAC GAGGACGCCT GAAGGAAGAG CTGGAAGAGG CTCGGGACAT AGCCCGGCGG
    CGCTCTTTAG GGAATATCAA GTTTATTGGA GAGTTGTTCA AACTGAAGAT GTTAACAGAG
    GCAATAATGC ATGACTGTGT GGTCAAACTG CTTAAGAACC ATGATGAAGA GTCCCTTGAG
    TGCCTTTGTC GTCTGCTCAC CACCATTGGC AAAGACCTGG ACTTTGAAAA AGCCAAGCCC
    CGAATGGATC AGTATTTCAA CCAGATGGAA AAAATCATTA AAGAAAAGAA GACGTCATCC
    CGCATCCGCT TTATGCTGCA GGACGTGCTG GATCTGCGAG GGAGCAATTG GGTGCCACGC
    CGAGGGGATC AGGGTCCCAA GACCATTGAC CAGATCCATA AGGAGGCTGA GATGGAAGAA
    CATCGAGAGC ACATCAAAGT GCAGCAGCTC ATGGCCAAGG GCAGTGACAA GCGTCGGGGC
    GGTCCTCCAG GCCCTCCCAT CAGCCGTGGA CTTCCCCTTG TGGATGATGG TGGCTGGAAC
    ACAGTTCCCA TCAGCAAAGG TAGCCGCCCC ATTGACACCT CACGACTCAC CAAGATCACC
    AAGCCTGGCT CCATCGATTC TAACAACCAG CTCTTTGCAC CTGGAGGGCG ACTGAGCTGG
    GGCAAGGGCA GCAGCGGAGG CTCAGGAGCC AAGCCCTCAG ACGCAGCATC AGAAGCTGCT
    CGCCCAGCTA CTAGTACTTT GAATCGCTTC TCAGCCCTTC AACAAGCGGT ACCCACAGAA
    AGCACAGATA ATAGACGTGT GGTGCAGAGG AGTAGCTTGA GCCGAGAACG AGGCGAGAAA
    GCTGGAGACC GAGGAGACCG CCTAGAGCGG AGTGAACGGG GAGGGGACCG TGGGGACCGG
    CTTGATCGTG CGCGGACACC TGCTACCAAG CGGAGCTTCA GCAAGGAAGT GGAGGAGCGG
    AGTAGAGAAC GGCCCTCCCA GCCTGAGGGG CTGCGCAAGG CAGCTAGCCT CACGGAGGAT
    CGGGACCGTG GGCGGGATGC CGTGAAGCGA GAAGCTGCCC TACCCCCAGT GAGCCCCCTG
    AAGGCGGCTC TCTCTGAGGA GGAGTTAGAG AAGAAATCCA AGGCTATCAT TGAGGAATAT
    CTCCATCTCA ATGACATGAA AGAGGCAGTC CAGTGCGTGC AGGAGCTGGC CTCACCCTCC
    TTGCTCTTCA TCTTTGTACG GCATGGTGTC GAGTCTACGC TGGAGCGCAG TGCCATTGCT
    CGTGAGCATA TGGGGCAGCT GCTGCACCAG CTGCTCTGTG CTGGGCATCT GTCTACTGCT
    CAGTACTACC AAGGGTTGTA TGAAATCTTG GAATTGGCTG AGGACATGGA AATTGACATC
    CCCCACGTGT GGCTCTACCT AGCGGAACTG GTAACACCCA TTCTGCAGGA AGGTGGGGTG
    CCCATGGGGG AGCTGTTCAG GGAGATTACA AAGCCTCTGA GACCGTTGGG CAAAGCTGCT
    TCCCTGTTGC TGGAGATCCT GGGCCTCCTG TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG
    ACGCTGTGGC GAGAAGCCGG GCTTAGCTGG AAGGAATTTC TACCTGAAGG CCAGGACATT
    GGTGCATTCG TCGCTGAACA GAAGGTGGAG TATACCCTGG GAGAGGAGTC GGAAGCCCCT
    GGCCAGAGGG CACTCCCCTC CGAGGAGCTG AACAGGCAGC TGGAGAAGCT GCTGAAGGAG
    GGCAGCAGTA ACCAGCGGGT GTTCGACTGG ATAGAGGCCA ACCTGAGTGA GCAGCAGATA
    GTATCCAACA CGTTAGTTCG AGCCCTCATG ACGGCTGTCT GCTATTCTGC AATTATTTTT
    GAGACTCCCC TCCGAGTGGA CGTTGCAGTG CTGAAAGCGC GAGCGAAGCT GCTGCAGAAA
    TACCTGTGTG ACGAGCAGAA GGAGCTACAG GCGCTCTACG CCCTCCAGGC CCTTGTAGTG
    ACCTTAGAAC AGCCTCCCAA CCTGCTGCGG ATGTTCTTTG ACGCACTGTA TGACGAGGAC
    GTGGTGAAGG AGGATGCCTT CTACAGTTGG GAGAGTAGCA AGGACCCCGC TGAGCAGCAG
    GGCAAGGGTG TGGCCCTTAA ATCTGTCACA GCCTTCTTCA AGTGGCTCCG TGAAGCAGAG
    GAGGAGTCTG ACCACAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108651
    Clone ID Related Accession (Same CDS sequence) NM_198241.3
    Accession Version NM_198241.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-20
    Organism Homo sapiens(human)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20. On Jun 2, 2019 this sequence version replaced NM_198241.2. Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (5) that is 1 aa shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX647812.1, SRR7346977.2441908.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAAAG CTCCACAGTC CACAGGCCCC CCACCCGCCC CATCCCCCGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCGCCG GTGGTGTTCA GTACGCCACA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG
    GGGGCCTACT ACATCCCTGG ACAGGGGCGT TCCACATACG TTGTCCCGAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACAGA ATTTGGGACC
    TACGCTGGCG CCTACTATCC AGCCCAAGGG GTGCAGCAGT TTCCCACTGG CGTGGCCCCC
    ACCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG TAAGACGATC
    CGAATTCGAG ATCCAAACCA AGGAGGAAAG GATATCACAG AGGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC CCCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAAGCTAAT
    GGGGAGACGC CCCAGGTTGC TGTCATTGTC CGGCCAGATG ACCGGTCACA GGGAGCAATC
    ATTGCTGACC GGCCAGGGCT GCCTGGCCCA GAGCATAGCC CTTCAGAATC CCAGCCTTCG
    TCGCCTTCTC CGACCCCATC ACCATCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC
    GCAGTCCTCT CTATTCCTGG GGACACTATG ACAACTATAC AAATGTCTGT AGAAGAATCA
    ACCCCCATCT CCCGTGAAAC TGGGGAGCCA TATCGCCTCT CTCCAGAACC CACTCCTCTC
    GCCGAACCCA TACTGGAAGT AGAAGTGACA CTTAGCAAAC CGGTTCCAGA ATCTGAGTTT
    TCTTCCAGTC CTCTCCAGGC TCCCACCCCT TTGGCATCTC ACACAGTGGA AATTCATGAG
    CCTAATGGCA TGGTCCCATC TGAAGATCTG GAACCAGAGG TGGAGTCAAG CCCAGAGCTT
    GCTCCTCCCC CAGCTTGCCC CTCCGAATCC CCTGTGCCCA TTGCTCCAAC TGCCCAACCT
    GAGGAACTGC TCAACGGAGC CCCCTCGCCA CCAGCTGTGG ACTTAAGCCC AGTCAGTGAG
    CCAGAGGAGC AGGCCAAGGA GGTGACAGCA TCAATGGCGC CCCCCACCAT CCCCTCTGCT
    ACTCCAGCTA CGGCTCCTTC AGCTACTTCC CCAGCTCAGG AGGAGGAAAT GGAAGAAGAA
    GAAGAAGAGG AAGAAGGAGA AGCAGGAGAA GCAGGAGAAG CTGAGAGTGA GAAAGGAGGA
    GAGGAACTGC TCCCCCCAGA GAGTACCCCT ATTCCAGCCA ACTTGTCTCA GAATTTGGAG
    GCAGCAGCAG CCACTCAAGT GGCAGTATCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG
    CTAAATAAGA AGGAGGCTGT TGGAGACCTT CTGGATGCCT TCAAGGAGGC GAACCCGGCA
    GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCAGCAATC CAGGCCCAGA GTCTGAGGGC
    AGTGGTGTGC CCCCACGTCC TGAGGAAGCA GATGAGACCT GGGACTCAAA GGAAGACAAA
    ATTCACAATG CTGAGAACAT CCAGCCCGGG GAACAGAAGT ATGAATATAA GTCAGATCAG
    TGGAAGCCTC TAAACCTAGA GGAGAAAAAA CGTTACGACC GTGAGTTCCT GCTTGGTTTT
    CAGTTCATCT TTGCCAGTAT GCAGAAGCCA GAGGGATTGC CACATATCAG TGACGTGGTG
    CTGGACAAGG CCAATAAAAC ACCACTGCGG CCACTGGATC CCACTAGACT ACAAGGCATA
    AATTGTGGCC CAGACTTCAC TCCATCCTTT GCCAACCTTG GCCGGACAAC CCTTAGCACC
    CGTGGGCCCC CAAGGGGTGG GCCAGGTGGG GAGCTGCCCC GTGGGCCGGC TGGCCTGGGA
    CCCCGGCGCT CTCAGCAGGG ACCCCGAAAA GAACCACGCA AGATCATTGC CACAGTGTTA
    ATGACCGAAG ATATAAAACT GAACAAAGCA GAGAAAGCCT GGAAACCCAG CAGCAAGCGG
    ACGGCGGCTG ATAAGGATCG AGGGGAAGAA GATGCTGATG GCAGCAAAAC CCAGGACCTA
    TTCCGCAGGG TGCGCTCCAT CCTGAATAAA CTGACACCCC AGATGTTCCA GCAGCTGATG
    AAGCAAGTGA CGCAGCTGGC CATCGACACC GAGGAACGCC TCAAAGGGGT CATTGACCTC
    ATTTTTGAGA AGGCCATTTC AGAGCCCAAC TTCTCTGTGG CCTATGCCAA CATGTGCCGC
    TGCCTCATGG CGCTGAAAGT GCCCACTACG GAAAAGCCAA CAGTGACTGT GAACTTCCGA
    AAGCTGTTGT TGAATCGATG TCAGAAGGAG TTTGAGAAAG ACAAAGATGA TGATGAGGTT
    TTTGAGAAGA AGCAAAAAGA GATGGATGAA GCTGCTACGG CAGAGGAACG AGGACGCCTG
    AAGGAAGAGC TGGAAGAGGC TCGGGACATA GCCCGGCGGC GCTCTTTAGG GAATATCAAG
    TTTATTGGAG AGTTGTTCAA ACTGAAGATG TTAACAGAGG CAATAATGCA TGACTGTGTG
    GTCAAACTGC TTAAGAACCA TGATGAAGAG TCCCTTGAGT GCCTTTGTCG TCTGCTCACC
    ACCATTGGCA AAGACCTGGA CTTTGAAAAA GCCAAGCCCC GAATGGATCA GTATTTCAAC
    CAGATGGAAA AAATCATTAA AGAAAAGAAG ACGTCATCCC GCATCCGCTT TATGCTGCAG
    GACGTGCTGG ATCTGCGAGG GAGCAATTGG GTGCCACGCC GAGGGGATCA GGGTCCCAAG
    ACCATTGACC AGATCCATAA GGAGGCTGAG ATGGAAGAAC ATCGAGAGCA CATCAAAGTG
    CAGCAGCTCA TGGCCAAGGG CAGTGACAAG CGTCGGGGCG GTCCTCCAGG CCCTCCCATC
    AGCCGTGGAC TTCCCCTTGT GGATGATGGT GGCTGGAACA CAGTTCCCAT CAGCAAAGGT
    AGCCGCCCCA TTGACACCTC ACGACTCACC AAGATCACCA AGCCTGGCTC CATCGATTCT
    AACAACCAGC TCTTTGCACC TGGAGGGCGA CTGAGCTGGG GCAAGGGCAG CAGCGGAGGC
    TCAGGAGCCA AGCCCTCAGA CGCAGCATCA GAAGCTGCTC GCCCAGCTAC TAGTACTTTG
    AATCGCTTCT CAGCCCTTCA ACAAGCGGTA CCCACAGAAA GCACAGATAA TAGACGTGTG
    GTGCAGAGGA GTAGCTTGAG CCGAGAACGA GGCGAGAAAG CTGGAGACCG AGGAGACCGC
    CTAGAGCGGA GTGAACGGGG AGGGGACCGT GGGGACCGGC TTGATCGTGC GCGGACACCT
    GCTACCAAGC GGAGCTTCAG CAAGGAAGTG GAGGAGCGGA GTAGAGAACG GCCCTCCCAG
    CCTGAGGGGC TGCGCAAGGC AGCTAGCCTC ACGGAGGATC GGGACCGTGG GCGGGATGCC
    GTGAAGCGAG AAGCTGCCCT ACCCCCAGTG AGCCCCCTGA AGGCGGCTCT CTCTGAGGAG
    GAGTTAGAGA AGAAATCCAA GGCTATCATT GAGGAATATC TCCATCTCAA TGACATGAAA
    GAGGCAGTCC AGTGCGTGCA GGAGCTGGCC TCACCCTCCT TGCTCTTCAT CTTTGTACGG
    CATGGTGTCG AGTCTACGCT GGAGCGCAGT GCCATTGCTC GTGAGCATAT GGGGCAGCTG
    CTGCACCAGC TGCTCTGTGC TGGGCATCTG TCTACTGCTC AGTACTACCA AGGGTTGTAT
    GAAATCTTGG AATTGGCTGA GGACATGGAA ATTGACATCC CCCACGTGTG GCTCTACCTA
    GCGGAACTGG TAACACCCAT TCTGCAGGAA GGTGGGGTGC CCATGGGGGA GCTGTTCAGG
    GAGATTACAA AGCCTCTGAG ACCGTTGGGC AAAGCTGCTT CCCTGTTGCT GGAGATCCTG
    GGCCTCCTGT GCAAAAGCAT GGGTCCTAAA AAGGTGGGGA CGCTGTGGCG AGAAGCCGGG
    CTTAGCTGGA AGGAATTTCT ACCTGAAGGC CAGGACATTG GTGCATTCGT CGCTGAACAG
    AAGGTGGAGT ATACCCTGGG AGAGGAGTCG GAAGCCCCTG GCCAGAGGGC ACTCCCCTCC
    GAGGAGCTGA ACAGGCAGCT GGAGAAGCTG CTGAAGGAGG GCAGCAGTAA CCAGCGGGTG
    TTCGACTGGA TAGAGGCCAA CCTGAGTGAG CAGCAGATAG TATCCAACAC GTTAGTTCGA
    GCCCTCATGA CGGCTGTCTG CTATTCTGCA ATTATTTTTG AGACTCCCCT CCGAGTGGAC
    GTTGCAGTGC TGAAAGCGCG AGCGAAGCTG CTGCAGAAAT ACCTGTGTGA CGAGCAGAAG
    GAGCTACAGG CGCTCTACGC CCTCCAGGCC CTTGTAGTGA CCTTAGAACA GCCTCCCAAC
    CTGCTGCGGA TGTTCTTTGA CGCACTGTAT GACGAGGACG TGGTGAAGGA GGATGCCTTC
    TACAGTTGGG AGAGTAGCAA GGACCCCGCT GAGCAGCAGG GCAAGGGTGT GGCCCTTAAA
    TCTGTCACAG CCTTCTTCAA GTGGCTCCGT GAAGCAGAGG AGGAGTCTGA CCACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_937884.2
    CDS161..4960
    Translation

    Target ORF information:

    RefSeq Version NM_198241.3
    Organism Homo sapiens(human)
    Definition Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_198241.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAAAG CTCCACAGTC CACAGGCCCC CCACCCGCCC CATCCCCCGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCGCCG GTGGTGTTCA GTACGCCACA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG
    GGGGCCTACT ACATCCCTGG ACAGGGGCGT TCCACATACG TTGTCCCGAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACAGA ATTTGGGACC
    TACGCTGGCG CCTACTATCC AGCCCAAGGG GTGCAGCAGT TTCCCACTGG CGTGGCCCCC
    ACCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG TAAGACGATC
    CGAATTCGAG ATCCAAACCA AGGAGGAAAG GATATCACAG AGGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC CCCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAAGCTAAT
    GGGGAGACGC CCCAGGTTGC TGTCATTGTC CGGCCAGATG ACCGGTCACA GGGAGCAATC
    ATTGCTGACC GGCCAGGGCT GCCTGGCCCA GAGCATAGCC CTTCAGAATC CCAGCCTTCG
    TCGCCTTCTC CGACCCCATC ACCATCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC
    GCAGTCCTCT CTATTCCTGG GGACACTATG ACAACTATAC AAATGTCTGT AGAAGAATCA
    ACCCCCATCT CCCGTGAAAC TGGGGAGCCA TATCGCCTCT CTCCAGAACC CACTCCTCTC
    GCCGAACCCA TACTGGAAGT AGAAGTGACA CTTAGCAAAC CGGTTCCAGA ATCTGAGTTT
    TCTTCCAGTC CTCTCCAGGC TCCCACCCCT TTGGCATCTC ACACAGTGGA AATTCATGAG
    CCTAATGGCA TGGTCCCATC TGAAGATCTG GAACCAGAGG TGGAGTCAAG CCCAGAGCTT
    GCTCCTCCCC CAGCTTGCCC CTCCGAATCC CCTGTGCCCA TTGCTCCAAC TGCCCAACCT
    GAGGAACTGC TCAACGGAGC CCCCTCGCCA CCAGCTGTGG ACTTAAGCCC AGTCAGTGAG
    CCAGAGGAGC AGGCCAAGGA GGTGACAGCA TCAATGGCGC CCCCCACCAT CCCCTCTGCT
    ACTCCAGCTA CGGCTCCTTC AGCTACTTCC CCAGCTCAGG AGGAGGAAAT GGAAGAAGAA
    GAAGAAGAGG AAGAAGGAGA AGCAGGAGAA GCAGGAGAAG CTGAGAGTGA GAAAGGAGGA
    GAGGAACTGC TCCCCCCAGA GAGTACCCCT ATTCCAGCCA ACTTGTCTCA GAATTTGGAG
    GCAGCAGCAG CCACTCAAGT GGCAGTATCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG
    CTAAATAAGA AGGAGGCTGT TGGAGACCTT CTGGATGCCT TCAAGGAGGC GAACCCGGCA
    GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCAGCAATC CAGGCCCAGA GTCTGAGGGC
    AGTGGTGTGC CCCCACGTCC TGAGGAAGCA GATGAGACCT GGGACTCAAA GGAAGACAAA
    ATTCACAATG CTGAGAACAT CCAGCCCGGG GAACAGAAGT ATGAATATAA GTCAGATCAG
    TGGAAGCCTC TAAACCTAGA GGAGAAAAAA CGTTACGACC GTGAGTTCCT GCTTGGTTTT
    CAGTTCATCT TTGCCAGTAT GCAGAAGCCA GAGGGATTGC CACATATCAG TGACGTGGTG
    CTGGACAAGG CCAATAAAAC ACCACTGCGG CCACTGGATC CCACTAGACT ACAAGGCATA
    AATTGTGGCC CAGACTTCAC TCCATCCTTT GCCAACCTTG GCCGGACAAC CCTTAGCACC
    CGTGGGCCCC CAAGGGGTGG GCCAGGTGGG GAGCTGCCCC GTGGGCCGGC TGGCCTGGGA
    CCCCGGCGCT CTCAGCAGGG ACCCCGAAAA GAACCACGCA AGATCATTGC CACAGTGTTA
    ATGACCGAAG ATATAAAACT GAACAAAGCA GAGAAAGCCT GGAAACCCAG CAGCAAGCGG
    ACGGCGGCTG ATAAGGATCG AGGGGAAGAA GATGCTGATG GCAGCAAAAC CCAGGACCTA
    TTCCGCAGGG TGCGCTCCAT CCTGAATAAA CTGACACCCC AGATGTTCCA GCAGCTGATG
    AAGCAAGTGA CGCAGCTGGC CATCGACACC GAGGAACGCC TCAAAGGGGT CATTGACCTC
    ATTTTTGAGA AGGCCATTTC AGAGCCCAAC TTCTCTGTGG CCTATGCCAA CATGTGCCGC
    TGCCTCATGG CGCTGAAAGT GCCCACTACG GAAAAGCCAA CAGTGACTGT GAACTTCCGA
    AAGCTGTTGT TGAATCGATG TCAGAAGGAG TTTGAGAAAG ACAAAGATGA TGATGAGGTT
    TTTGAGAAGA AGCAAAAAGA GATGGATGAA GCTGCTACGG CAGAGGAACG AGGACGCCTG
    AAGGAAGAGC TGGAAGAGGC TCGGGACATA GCCCGGCGGC GCTCTTTAGG GAATATCAAG
    TTTATTGGAG AGTTGTTCAA ACTGAAGATG TTAACAGAGG CAATAATGCA TGACTGTGTG
    GTCAAACTGC TTAAGAACCA TGATGAAGAG TCCCTTGAGT GCCTTTGTCG TCTGCTCACC
    ACCATTGGCA AAGACCTGGA CTTTGAAAAA GCCAAGCCCC GAATGGATCA GTATTTCAAC
    CAGATGGAAA AAATCATTAA AGAAAAGAAG ACGTCATCCC GCATCCGCTT TATGCTGCAG
    GACGTGCTGG ATCTGCGAGG GAGCAATTGG GTGCCACGCC GAGGGGATCA GGGTCCCAAG
    ACCATTGACC AGATCCATAA GGAGGCTGAG ATGGAAGAAC ATCGAGAGCA CATCAAAGTG
    CAGCAGCTCA TGGCCAAGGG CAGTGACAAG CGTCGGGGCG GTCCTCCAGG CCCTCCCATC
    AGCCGTGGAC TTCCCCTTGT GGATGATGGT GGCTGGAACA CAGTTCCCAT CAGCAAAGGT
    AGCCGCCCCA TTGACACCTC ACGACTCACC AAGATCACCA AGCCTGGCTC CATCGATTCT
    AACAACCAGC TCTTTGCACC TGGAGGGCGA CTGAGCTGGG GCAAGGGCAG CAGCGGAGGC
    TCAGGAGCCA AGCCCTCAGA CGCAGCATCA GAAGCTGCTC GCCCAGCTAC TAGTACTTTG
    AATCGCTTCT CAGCCCTTCA ACAAGCGGTA CCCACAGAAA GCACAGATAA TAGACGTGTG
    GTGCAGAGGA GTAGCTTGAG CCGAGAACGA GGCGAGAAAG CTGGAGACCG AGGAGACCGC
    CTAGAGCGGA GTGAACGGGG AGGGGACCGT GGGGACCGGC TTGATCGTGC GCGGACACCT
    GCTACCAAGC GGAGCTTCAG CAAGGAAGTG GAGGAGCGGA GTAGAGAACG GCCCTCCCAG
    CCTGAGGGGC TGCGCAAGGC AGCTAGCCTC ACGGAGGATC GGGACCGTGG GCGGGATGCC
    GTGAAGCGAG AAGCTGCCCT ACCCCCAGTG AGCCCCCTGA AGGCGGCTCT CTCTGAGGAG
    GAGTTAGAGA AGAAATCCAA GGCTATCATT GAGGAATATC TCCATCTCAA TGACATGAAA
    GAGGCAGTCC AGTGCGTGCA GGAGCTGGCC TCACCCTCCT TGCTCTTCAT CTTTGTACGG
    CATGGTGTCG AGTCTACGCT GGAGCGCAGT GCCATTGCTC GTGAGCATAT GGGGCAGCTG
    CTGCACCAGC TGCTCTGTGC TGGGCATCTG TCTACTGCTC AGTACTACCA AGGGTTGTAT
    GAAATCTTGG AATTGGCTGA GGACATGGAA ATTGACATCC CCCACGTGTG GCTCTACCTA
    GCGGAACTGG TAACACCCAT TCTGCAGGAA GGTGGGGTGC CCATGGGGGA GCTGTTCAGG
    GAGATTACAA AGCCTCTGAG ACCGTTGGGC AAAGCTGCTT CCCTGTTGCT GGAGATCCTG
    GGCCTCCTGT GCAAAAGCAT GGGTCCTAAA AAGGTGGGGA CGCTGTGGCG AGAAGCCGGG
    CTTAGCTGGA AGGAATTTCT ACCTGAAGGC CAGGACATTG GTGCATTCGT CGCTGAACAG
    AAGGTGGAGT ATACCCTGGG AGAGGAGTCG GAAGCCCCTG GCCAGAGGGC ACTCCCCTCC
    GAGGAGCTGA ACAGGCAGCT GGAGAAGCTG CTGAAGGAGG GCAGCAGTAA CCAGCGGGTG
    TTCGACTGGA TAGAGGCCAA CCTGAGTGAG CAGCAGATAG TATCCAACAC GTTAGTTCGA
    GCCCTCATGA CGGCTGTCTG CTATTCTGCA ATTATTTTTG AGACTCCCCT CCGAGTGGAC
    GTTGCAGTGC TGAAAGCGCG AGCGAAGCTG CTGCAGAAAT ACCTGTGTGA CGAGCAGAAG
    GAGCTACAGG CGCTCTACGC CCTCCAGGCC CTTGTAGTGA CCTTAGAACA GCCTCCCAAC
    CTGCTGCGGA TGTTCTTTGA CGCACTGTAT GACGAGGACG TGGTGAAGGA GGATGCCTTC
    TACAGTTGGG AGAGTAGCAA GGACCCCGCT GAGCAGCAGG GCAAGGGTGT GGCCCTTAAA
    TCTGTCACAG CCTTCTTCAA GTGGCTCCGT GAAGCAGAGG AGGAGTCTGA CCACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108653
    Clone ID Related Accession (Same CDS sequence) NM_001291157.2
    Accession Version NM_001291157.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-29
    Organism Homo sapiens(human)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform 7
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20. On Jun 2, 2019 this sequence version replaced NM_001291157.1. Transcript Variant: This variant (8) differs in the 5' UTR, includes an additional internal segment in the 5' region, which results in a downstream start codon, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (7) is shorter at the N-terminus, and lacks an internal residue, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK226160.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC 
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG
    GGGGCCTACT ACATCCCTGG ACAGGGGCGT TCCACATACG TTGTCCCGAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACAGA ATTTGGGACC
    TACGCTGGCG CCTACTATCC AGCCCAAGGG GTGCAGCAGT TTCCCACTGG CGTGGCCCCC
    ACCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG TAAGACGATC
    CGAATTCGAG ATCCAAACCA AGGAGGAAAG GATATCACAG AGGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC CCCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAAGCTAAT
    GGGGAGACGC CCCAGGTTGC TGTCATTGTC CGGCCAGATG ACCGGTCACA GGGAGCAATC
    ATTGCTGACC GGCCAGGGCT GCCTGGCCCA GAGCATAGCC CTTCAGAATC CCAGCCTTCG
    TCGCCTTCTC CGACCCCATC ACCATCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC
    GCAGTCCTCT CTATTCCTGG GGACACTATG ACAACTATAC AAATGTCTGT AGAAGAATCA
    ACCCCCATCT CCCGTGAAAC TGGGGAGCCA TATCGCCTCT CTCCAGAACC CACTCCTCTC
    GCCGAACCCA TACTGGAAGT AGAAGTGACA CTTAGCAAAC CGGTTCCAGA ATCTGAGTTT
    TCTTCCAGTC CTCTCCAGGC TCCCACCCCT TTGGCATCTC ACACAGTGGA AATTCATGAG
    CCTAATGGCA TGGTCCCATC TGAAGATCTG GAACCAGAGG TGGAGTCAAG CCCAGAGCTT
    GCTCCTCCCC CAGCTTGCCC CTCCGAATCC CCTGTGCCCA TTGCTCCAAC TGCCCAACCT
    GAGGAACTGC TCAACGGAGC CCCCTCGCCA CCAGCTGTGG ACTTAAGCCC AGTCAGTGAG
    CCAGAGGAGC AGGCCAAGGA GGTGACAGCA TCAATGGCGC CCCCCACCAT CCCCTCTGCT
    ACTCCAGCTA CGGCTCCTTC AGCTACTTCC CCAGCTCAGG AGGAGGAAAT GGAAGAAGAA
    GAAGAAGAGG AAGAAGGAGA AGCAGGAGAA GCAGGAGAAG CTGAGAGTGA GAAAGGAGGA
    GAGGAACTGC TCCCCCCAGA GAGTACCCCT ATTCCAGCCA ACTTGTCTCA GAATTTGGAG
    GCAGCAGCAG CCACTCAAGT GGCAGTATCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG
    CTAAATAAGA AGGAGGCTGT TGGAGACCTT CTGGATGCCT TCAAGGAGGC GAACCCGGCA
    GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCAGCAATC CAGGCCCAGA GTCTGAGGGC
    AGTGGTGTGC CCCCACGTCC TGAGGAAGCA GATGAGACCT GGGACTCAAA GGAAGACAAA
    ATTCACAATG CTGAGAACAT CCAGCCCGGG GAACAGAAGT ATGAATATAA GTCAGATCAG
    TGGAAGCCTC TAAACCTAGA GGAGAAAAAA CGTTACGACC GTGAGTTCCT GCTTGGTTTT
    CAGTTCATCT TTGCCAGTAT GCAGAAGCCA GAGGGATTGC CACATATCAG TGACGTGGTG
    CTGGACAAGG CCAATAAAAC ACCACTGCGG CCACTGGATC CCACTAGACT ACAAGGCATA
    AATTGTGGCC CAGACTTCAC TCCATCCTTT GCCAACCTTG GCCGGACAAC CCTTAGCACC
    CGTGGGCCCC CAAGGGGTGG GCCAGGTGGG GAGCTGCCCC GTGGGCCGGC TGGCCTGGGA
    CCCCGGCGCT CTCAGCAGGG ACCCCGAAAA GAACCACGCA AGATCATTGC CACAGTGTTA
    ATGACCGAAG ATATAAAACT GAACAAAGCA GAGAAAGCCT GGAAACCCAG CAGCAAGCGG
    ACGGCGGCTG ATAAGGATCG AGGGGAAGAA GATGCTGATG GCAGCAAAAC CCAGGACCTA
    TTCCGCAGGG TGCGCTCCAT CCTGAATAAA CTGACACCCC AGATGTTCCA GCAGCTGATG
    AAGCAAGTGA CGCAGCTGGC CATCGACACC GAGGAACGCC TCAAAGGGGT CATTGACCTC
    ATTTTTGAGA AGGCCATTTC AGAGCCCAAC TTCTCTGTGG CCTATGCCAA CATGTGCCGC
    TGCCTCATGG CGCTGAAAGT GCCCACTACG GAAAAGCCAA CAGTGACTGT GAACTTCCGA
    AAGCTGTTGT TGAATCGATG TCAGAAGGAG TTTGAGAAAG ACAAAGATGA TGATGAGGTT
    TTTGAGAAGA AGCAAAAAGA GATGGATGAA GCTGCTACGG CAGAGGAACG AGGACGCCTG
    AAGGAAGAGC TGGAAGAGGC TCGGGACATA GCCCGGCGGC GCTCTTTAGG GAATATCAAG
    TTTATTGGAG AGTTGTTCAA ACTGAAGATG TTAACAGAGG CAATAATGCA TGACTGTGTG
    GTCAAACTGC TTAAGAACCA TGATGAAGAG TCCCTTGAGT GCCTTTGTCG TCTGCTCACC
    ACCATTGGCA AAGACCTGGA CTTTGAAAAA GCCAAGCCCC GAATGGATCA GTATTTCAAC
    CAGATGGAAA AAATCATTAA AGAAAAGAAG ACGTCATCCC GCATCCGCTT TATGCTGCAG
    GACGTGCTGG ATCTGCGAGG GAGCAATTGG GTGCCACGCC GAGGGGATCA GGGTCCCAAG
    ACCATTGACC AGATCCATAA GGAGGCTGAG ATGGAAGAAC ATCGAGAGCA CATCAAAGTG
    CAGCAGCTCA TGGCCAAGGG CAGTGACAAG CGTCGGGGCG GTCCTCCAGG CCCTCCCATC
    AGCCGTGGAC TTCCCCTTGT GGATGATGGT GGCTGGAACA CAGTTCCCAT CAGCAAAGGT
    AGCCGCCCCA TTGACACCTC ACGACTCACC AAGATCACCA AGCCTGGCTC CATCGATTCT
    AACAACCAGC TCTTTGCACC TGGAGGGCGA CTGAGCTGGG GCAAGGGCAG CAGCGGAGGC
    TCAGGAGCCA AGCCCTCAGA CGCAGCATCA GAAGCTGCTC GCCCAGCTAC TAGTACTTTG
    AATCGCTTCT CAGCCCTTCA ACAAGCGGTA CCCACAGAAA GCACAGATAA TAGACGTGTG
    GTGCAGAGGA GTAGCTTGAG CCGAGAACGA GGCGAGAAAG CTGGAGACCG AGGAGACCGC
    CTAGAGCGGA GTGAACGGGG AGGGGACCGT GGGGACCGGC TTGATCGTGC GCGGACACCT
    GCTACCAAGC GGAGCTTCAG CAAGGAAGTG GAGGAGCGGA GTAGAGAACG GCCCTCCCAG
    CCTGAGGGGC TGCGCAAGGC AGCTAGCCTC ACGGAGGATC GGGACCGTGG GCGGGATGCC
    GTGAAGCGAG AAGCTGCCCT ACCCCCAGTG AGCCCCCTGA AGGCGGCTCT CTCTGAGGAG
    GAGTTAGAGA AGAAATCCAA GGCTATCATT GAGGAATATC TCCATCTCAA TGACATGAAA
    GAGGCAGTCC AGTGCGTGCA GGAGCTGGCC TCACCCTCCT TGCTCTTCAT CTTTGTACGG
    CATGGTGTCG AGTCTACGCT GGAGCGCAGT GCCATTGCTC GTGAGCATAT GGGGCAGCTG
    CTGCACCAGC TGCTCTGTGC TGGGCATCTG TCTACTGCTC AGTACTACCA AGGGTTGTAT
    GAAATCTTGG AATTGGCTGA GGACATGGAA ATTGACATCC CCCACGTGTG GCTCTACCTA
    GCGGAACTGG TAACACCCAT TCTGCAGGAA GGTGGGGTGC CCATGGGGGA GCTGTTCAGG
    GAGATTACAA AGCCTCTGAG ACCGTTGGGC AAAGCTGCTT CCCTGTTGCT GGAGATCCTG
    GGCCTCCTGT GCAAAAGCAT GGGTCCTAAA AAGGTGGGGA CGCTGTGGCG AGAAGCCGGG
    CTTAGCTGGA AGGAATTTCT ACCTGAAGGC CAGGACATTG GTGCATTCGT CGCTGAACAG
    AAGGTGGAGT ATACCCTGGG AGAGGAGTCG GAAGCCCCTG GCCAGAGGGC ACTCCCCTCC
    GAGGAGCTGA ACAGGCAGCT GGAGAAGCTG CTGAAGGAGG GCAGCAGTAA CCAGCGGGTG
    TTCGACTGGA TAGAGGCCAA CCTGAGTGAG CAGCAGATAG TATCCAACAC GTTAGTTCGA
    GCCCTCATGA CGGCTGTCTG CTATTCTGCA ATTATTTTTG AGACTCCCCT CCGAGTGGAC
    GTTGCAGTGC TGAAAGCGCG AGCGAAGCTG CTGCAGAAAT ACCTGTGTGA CGAGCAGAAG
    GAGCTACAGG CGCTCTACGC CCTCCAGGCC CTTGTAGTGA CCTTAGAACA GCCTCCCAAC
    CTGCTGCGGA TGTTCTTTGA CGCACTGTAT GACGAGGACG TGGTGAAGGA GGATGCCTTC
    TACAGTTGGG AGAGTAGCAA GGACCCCGCT GAGCAGCAGG GCAAGGGTGT GGCCCTTAAA
    TCTGTCACAG CCTTCTTCAA GTGGCTCCGT GAAGCAGAGG AGGAGTCTGA CCACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001278086.2
    CDS341..5020
    Translation

    Target ORF information:

    RefSeq Version NM_001291157.2
    Organism Homo sapiens(human)
    Definition Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001291157.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC 
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG
    GGGGCCTACT ACATCCCTGG ACAGGGGCGT TCCACATACG TTGTCCCGAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACAGA ATTTGGGACC
    TACGCTGGCG CCTACTATCC AGCCCAAGGG GTGCAGCAGT TTCCCACTGG CGTGGCCCCC
    ACCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG TAAGACGATC
    CGAATTCGAG ATCCAAACCA AGGAGGAAAG GATATCACAG AGGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC CCCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAAGCTAAT
    GGGGAGACGC CCCAGGTTGC TGTCATTGTC CGGCCAGATG ACCGGTCACA GGGAGCAATC
    ATTGCTGACC GGCCAGGGCT GCCTGGCCCA GAGCATAGCC CTTCAGAATC CCAGCCTTCG
    TCGCCTTCTC CGACCCCATC ACCATCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC
    GCAGTCCTCT CTATTCCTGG GGACACTATG ACAACTATAC AAATGTCTGT AGAAGAATCA
    ACCCCCATCT CCCGTGAAAC TGGGGAGCCA TATCGCCTCT CTCCAGAACC CACTCCTCTC
    GCCGAACCCA TACTGGAAGT AGAAGTGACA CTTAGCAAAC CGGTTCCAGA ATCTGAGTTT
    TCTTCCAGTC CTCTCCAGGC TCCCACCCCT TTGGCATCTC ACACAGTGGA AATTCATGAG
    CCTAATGGCA TGGTCCCATC TGAAGATCTG GAACCAGAGG TGGAGTCAAG CCCAGAGCTT
    GCTCCTCCCC CAGCTTGCCC CTCCGAATCC CCTGTGCCCA TTGCTCCAAC TGCCCAACCT
    GAGGAACTGC TCAACGGAGC CCCCTCGCCA CCAGCTGTGG ACTTAAGCCC AGTCAGTGAG
    CCAGAGGAGC AGGCCAAGGA GGTGACAGCA TCAATGGCGC CCCCCACCAT CCCCTCTGCT
    ACTCCAGCTA CGGCTCCTTC AGCTACTTCC CCAGCTCAGG AGGAGGAAAT GGAAGAAGAA
    GAAGAAGAGG AAGAAGGAGA AGCAGGAGAA GCAGGAGAAG CTGAGAGTGA GAAAGGAGGA
    GAGGAACTGC TCCCCCCAGA GAGTACCCCT ATTCCAGCCA ACTTGTCTCA GAATTTGGAG
    GCAGCAGCAG CCACTCAAGT GGCAGTATCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG
    CTAAATAAGA AGGAGGCTGT TGGAGACCTT CTGGATGCCT TCAAGGAGGC GAACCCGGCA
    GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCAGCAATC CAGGCCCAGA GTCTGAGGGC
    AGTGGTGTGC CCCCACGTCC TGAGGAAGCA GATGAGACCT GGGACTCAAA GGAAGACAAA
    ATTCACAATG CTGAGAACAT CCAGCCCGGG GAACAGAAGT ATGAATATAA GTCAGATCAG
    TGGAAGCCTC TAAACCTAGA GGAGAAAAAA CGTTACGACC GTGAGTTCCT GCTTGGTTTT
    CAGTTCATCT TTGCCAGTAT GCAGAAGCCA GAGGGATTGC CACATATCAG TGACGTGGTG
    CTGGACAAGG CCAATAAAAC ACCACTGCGG CCACTGGATC CCACTAGACT ACAAGGCATA
    AATTGTGGCC CAGACTTCAC TCCATCCTTT GCCAACCTTG GCCGGACAAC CCTTAGCACC
    CGTGGGCCCC CAAGGGGTGG GCCAGGTGGG GAGCTGCCCC GTGGGCCGGC TGGCCTGGGA
    CCCCGGCGCT CTCAGCAGGG ACCCCGAAAA GAACCACGCA AGATCATTGC CACAGTGTTA
    ATGACCGAAG ATATAAAACT GAACAAAGCA GAGAAAGCCT GGAAACCCAG CAGCAAGCGG
    ACGGCGGCTG ATAAGGATCG AGGGGAAGAA GATGCTGATG GCAGCAAAAC CCAGGACCTA
    TTCCGCAGGG TGCGCTCCAT CCTGAATAAA CTGACACCCC AGATGTTCCA GCAGCTGATG
    AAGCAAGTGA CGCAGCTGGC CATCGACACC GAGGAACGCC TCAAAGGGGT CATTGACCTC
    ATTTTTGAGA AGGCCATTTC AGAGCCCAAC TTCTCTGTGG CCTATGCCAA CATGTGCCGC
    TGCCTCATGG CGCTGAAAGT GCCCACTACG GAAAAGCCAA CAGTGACTGT GAACTTCCGA
    AAGCTGTTGT TGAATCGATG TCAGAAGGAG TTTGAGAAAG ACAAAGATGA TGATGAGGTT
    TTTGAGAAGA AGCAAAAAGA GATGGATGAA GCTGCTACGG CAGAGGAACG AGGACGCCTG
    AAGGAAGAGC TGGAAGAGGC TCGGGACATA GCCCGGCGGC GCTCTTTAGG GAATATCAAG
    TTTATTGGAG AGTTGTTCAA ACTGAAGATG TTAACAGAGG CAATAATGCA TGACTGTGTG
    GTCAAACTGC TTAAGAACCA TGATGAAGAG TCCCTTGAGT GCCTTTGTCG TCTGCTCACC
    ACCATTGGCA AAGACCTGGA CTTTGAAAAA GCCAAGCCCC GAATGGATCA GTATTTCAAC
    CAGATGGAAA AAATCATTAA AGAAAAGAAG ACGTCATCCC GCATCCGCTT TATGCTGCAG
    GACGTGCTGG ATCTGCGAGG GAGCAATTGG GTGCCACGCC GAGGGGATCA GGGTCCCAAG
    ACCATTGACC AGATCCATAA GGAGGCTGAG ATGGAAGAAC ATCGAGAGCA CATCAAAGTG
    CAGCAGCTCA TGGCCAAGGG CAGTGACAAG CGTCGGGGCG GTCCTCCAGG CCCTCCCATC
    AGCCGTGGAC TTCCCCTTGT GGATGATGGT GGCTGGAACA CAGTTCCCAT CAGCAAAGGT
    AGCCGCCCCA TTGACACCTC ACGACTCACC AAGATCACCA AGCCTGGCTC CATCGATTCT
    AACAACCAGC TCTTTGCACC TGGAGGGCGA CTGAGCTGGG GCAAGGGCAG CAGCGGAGGC
    TCAGGAGCCA AGCCCTCAGA CGCAGCATCA GAAGCTGCTC GCCCAGCTAC TAGTACTTTG
    AATCGCTTCT CAGCCCTTCA ACAAGCGGTA CCCACAGAAA GCACAGATAA TAGACGTGTG
    GTGCAGAGGA GTAGCTTGAG CCGAGAACGA GGCGAGAAAG CTGGAGACCG AGGAGACCGC
    CTAGAGCGGA GTGAACGGGG AGGGGACCGT GGGGACCGGC TTGATCGTGC GCGGACACCT
    GCTACCAAGC GGAGCTTCAG CAAGGAAGTG GAGGAGCGGA GTAGAGAACG GCCCTCCCAG
    CCTGAGGGGC TGCGCAAGGC AGCTAGCCTC ACGGAGGATC GGGACCGTGG GCGGGATGCC
    GTGAAGCGAG AAGCTGCCCT ACCCCCAGTG AGCCCCCTGA AGGCGGCTCT CTCTGAGGAG
    GAGTTAGAGA AGAAATCCAA GGCTATCATT GAGGAATATC TCCATCTCAA TGACATGAAA
    GAGGCAGTCC AGTGCGTGCA GGAGCTGGCC TCACCCTCCT TGCTCTTCAT CTTTGTACGG
    CATGGTGTCG AGTCTACGCT GGAGCGCAGT GCCATTGCTC GTGAGCATAT GGGGCAGCTG
    CTGCACCAGC TGCTCTGTGC TGGGCATCTG TCTACTGCTC AGTACTACCA AGGGTTGTAT
    GAAATCTTGG AATTGGCTGA GGACATGGAA ATTGACATCC CCCACGTGTG GCTCTACCTA
    GCGGAACTGG TAACACCCAT TCTGCAGGAA GGTGGGGTGC CCATGGGGGA GCTGTTCAGG
    GAGATTACAA AGCCTCTGAG ACCGTTGGGC AAAGCTGCTT CCCTGTTGCT GGAGATCCTG
    GGCCTCCTGT GCAAAAGCAT GGGTCCTAAA AAGGTGGGGA CGCTGTGGCG AGAAGCCGGG
    CTTAGCTGGA AGGAATTTCT ACCTGAAGGC CAGGACATTG GTGCATTCGT CGCTGAACAG
    AAGGTGGAGT ATACCCTGGG AGAGGAGTCG GAAGCCCCTG GCCAGAGGGC ACTCCCCTCC
    GAGGAGCTGA ACAGGCAGCT GGAGAAGCTG CTGAAGGAGG GCAGCAGTAA CCAGCGGGTG
    TTCGACTGGA TAGAGGCCAA CCTGAGTGAG CAGCAGATAG TATCCAACAC GTTAGTTCGA
    GCCCTCATGA CGGCTGTCTG CTATTCTGCA ATTATTTTTG AGACTCCCCT CCGAGTGGAC
    GTTGCAGTGC TGAAAGCGCG AGCGAAGCTG CTGCAGAAAT ACCTGTGTGA CGAGCAGAAG
    GAGCTACAGG CGCTCTACGC CCTCCAGGCC CTTGTAGTGA CCTTAGAACA GCCTCCCAAC
    CTGCTGCGGA TGTTCTTTGA CGCACTGTAT GACGAGGACG TGGTGAAGGA GGATGCCTTC
    TACAGTTGGG AGAGTAGCAA GGACCCCGCT GAGCAGCAGG GCAAGGGTGT GGCCCTTAAA
    TCTGTCACAG CCTTCTTCAA GTGGCTCCGT GAAGCAGAGG AGGAGTCTGA CCACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108656
    Clone ID Related Accession (Same CDS sequence) NM_198244.3
    Accession Version NM_198244.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4539bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-29
    Organism Homo sapiens(human)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20. On Jun 2, 2019 this sequence version replaced NM_198244.2. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, uses a downstream start codon, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (2) is shorter at the N-terminus and lacks an internal residue, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK299763.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATGATCC CTTCCCAGAT CTCCTACCCA GCCTCCCAGG GGGCCTACTA CATCCCTGGA 
    CAGGGGCGTT CCACATACGT TGTCCCGACA CAGCAGTACC CTGTGCAGCC AGGAGCCCCA
    GGCTTCTATC CAGGTGCAAG CCCTACAGAA TTTGGGACCT ACGCTGGCGC CTACTATCCA
    GCCCAAGGGG TGCAGCAGTT TCCCACTGGC GTGGCCCCCA CCCCAGTTTT GATGAACCAG
    CCACCCCAGA TTGCTCCCAA GAGGGAGCGT AAGACGATCC GAATTCGAGA TCCAAACCAA
    GGAGGAAAGG ATATCACAGA GGAGATCATG TCTGGGGCCC GCACTGCCTC CACACCCACC
    CCTCCCCAGA CGGGAGGCGG TCTGGAGCCT CAAGCTAATG GGGAGACGCC CCAGGTTGCT
    GTCATTGTCC GGCCAGATGA CCGGTCACAG GGAGCAATCA TTGCTGACCG GCCAGGGCTG
    CCTGGCCCAG AGCATAGCCC TTCAGAATCC CAGCCTTCGT CGCCTTCTCC GACCCCATCA
    CCATCCCCAG TCTTGGAACC GGGGTCTGAG CCTAATCTCG CAGTCCTCTC TATTCCTGGG
    GACACTATGA CAACTATACA AATGTCTGTA GAAGAATCAA CCCCCATCTC CCGTGAAACT
    GGGGAGCCAT ATCGCCTCTC TCCAGAACCC ACTCCTCTCG CCGAACCCAT ACTGGAAGTA
    GAAGTGACAC TTAGCAAACC GGTTCCAGAA TCTGAGTTTT CTTCCAGTCC TCTCCAGGCT
    CCCACCCCTT TGGCATCTCA CACAGTGGAA ATTCATGAGC CTAATGGCAT GGTCCCATCT
    GAAGATCTGG AACCAGAGGT GGAGTCAAGC CCAGAGCTTG CTCCTCCCCC AGCTTGCCCC
    TCCGAATCCC CTGTGCCCAT TGCTCCAACT GCCCAACCTG AGGAACTGCT CAACGGAGCC
    CCCTCGCCAC CAGCTGTGGA CTTAAGCCCA GTCAGTGAGC CAGAGGAGCA GGCCAAGGAG
    GTGACAGCAT CAATGGCGCC CCCCACCATC CCCTCTGCTA CTCCAGCTAC GGCTCCTTCA
    GCTACTTCCC CAGCTCAGGA GGAGGAAATG GAAGAAGAAG AAGAAGAGGA AGAAGGAGAA
    GCAGGAGAAG CAGGAGAAGC TGAGAGTGAG AAAGGAGGAG AGGAACTGCT CCCCCCAGAG
    AGTACCCCTA TTCCAGCCAA CTTGTCTCAG AATTTGGAGG CAGCAGCAGC CACTCAAGTG
    GCAGTATCTG TGCCAAAGAG GAGACGGAAA ATTAAGGAGC TAAATAAGAA GGAGGCTGTT
    GGAGACCTTC TGGATGCCTT CAAGGAGGCG AACCCGGCAG TACCAGAGGT GGAAAATCAG
    CCTCCTGCAG GCAGCAATCC AGGCCCAGAG TCTGAGGGCA GTGGTGTGCC CCCACGTCCT
    GAGGAAGCAG ATGAGACCTG GGACTCAAAG GAAGACAAAA TTCACAATGC TGAGAACATC
    CAGCCCGGGG AACAGAAGTA TGAATATAAG TCAGATCAGT GGAAGCCTCT AAACCTAGAG
    GAGAAAAAAC GTTACGACCG TGAGTTCCTG CTTGGTTTTC AGTTCATCTT TGCCAGTATG
    CAGAAGCCAG AGGGATTGCC ACATATCAGT GACGTGGTGC TGGACAAGGC CAATAAAACA
    CCACTGCGGC CACTGGATCC CACTAGACTA CAAGGCATAA ATTGTGGCCC AGACTTCACT
    CCATCCTTTG CCAACCTTGG CCGGACAACC CTTAGCACCC GTGGGCCCCC AAGGGGTGGG
    CCAGGTGGGG AGCTGCCCCG TGGGCCGGCT GGCCTGGGAC CCCGGCGCTC TCAGCAGGGA
    CCCCGAAAAG AACCACGCAA GATCATTGCC ACAGTGTTAA TGACCGAAGA TATAAAACTG
    AACAAAGCAG AGAAAGCCTG GAAACCCAGC AGCAAGCGGA CGGCGGCTGA TAAGGATCGA
    GGGGAAGAAG ATGCTGATGG CAGCAAAACC CAGGACCTAT TCCGCAGGGT GCGCTCCATC
    CTGAATAAAC TGACACCCCA GATGTTCCAG CAGCTGATGA AGCAAGTGAC GCAGCTGGCC
    ATCGACACCG AGGAACGCCT CAAAGGGGTC ATTGACCTCA TTTTTGAGAA GGCCATTTCA
    GAGCCCAACT TCTCTGTGGC CTATGCCAAC ATGTGCCGCT GCCTCATGGC GCTGAAAGTG
    CCCACTACGG AAAAGCCAAC AGTGACTGTG AACTTCCGAA AGCTGTTGTT GAATCGATGT
    CAGAAGGAGT TTGAGAAAGA CAAAGATGAT GATGAGGTTT TTGAGAAGAA GCAAAAAGAG
    ATGGATGAAG CTGCTACGGC AGAGGAACGA GGACGCCTGA AGGAAGAGCT GGAAGAGGCT
    CGGGACATAG CCCGGCGGCG CTCTTTAGGG AATATCAAGT TTATTGGAGA GTTGTTCAAA
    CTGAAGATGT TAACAGAGGC AATAATGCAT GACTGTGTGG TCAAACTGCT TAAGAACCAT
    GATGAAGAGT CCCTTGAGTG CCTTTGTCGT CTGCTCACCA CCATTGGCAA AGACCTGGAC
    TTTGAAAAAG CCAAGCCCCG AATGGATCAG TATTTCAACC AGATGGAAAA AATCATTAAA
    GAAAAGAAGA CGTCATCCCG CATCCGCTTT ATGCTGCAGG ACGTGCTGGA TCTGCGAGGG
    AGCAATTGGG TGCCACGCCG AGGGGATCAG GGTCCCAAGA CCATTGACCA GATCCATAAG
    GAGGCTGAGA TGGAAGAACA TCGAGAGCAC ATCAAAGTGC AGCAGCTCAT GGCCAAGGGC
    AGTGACAAGC GTCGGGGCGG TCCTCCAGGC CCTCCCATCA GCCGTGGACT TCCCCTTGTG
    GATGATGGTG GCTGGAACAC AGTTCCCATC AGCAAAGGTA GCCGCCCCAT TGACACCTCA
    CGACTCACCA AGATCACCAA GCCTGGCTCC ATCGATTCTA ACAACCAGCT CTTTGCACCT
    GGAGGGCGAC TGAGCTGGGG CAAGGGCAGC AGCGGAGGCT CAGGAGCCAA GCCCTCAGAC
    GCAGCATCAG AAGCTGCTCG CCCAGCTACT AGTACTTTGA ATCGCTTCTC AGCCCTTCAA
    CAAGCGGTAC CCACAGAAAG CACAGATAAT AGACGTGTGG TGCAGAGGAG TAGCTTGAGC
    CGAGAACGAG GCGAGAAAGC TGGAGACCGA GGAGACCGCC TAGAGCGGAG TGAACGGGGA
    GGGGACCGTG GGGACCGGCT TGATCGTGCG CGGACACCTG CTACCAAGCG GAGCTTCAGC
    AAGGAAGTGG AGGAGCGGAG TAGAGAACGG CCCTCCCAGC CTGAGGGGCT GCGCAAGGCA
    GCTAGCCTCA CGGAGGATCG GGACCGTGGG CGGGATGCCG TGAAGCGAGA AGCTGCCCTA
    CCCCCAGTGA GCCCCCTGAA GGCGGCTCTC TCTGAGGAGG AGTTAGAGAA GAAATCCAAG
    GCTATCATTG AGGAATATCT CCATCTCAAT GACATGAAAG AGGCAGTCCA GTGCGTGCAG
    GAGCTGGCCT CACCCTCCTT GCTCTTCATC TTTGTACGGC ATGGTGTCGA GTCTACGCTG
    GAGCGCAGTG CCATTGCTCG TGAGCATATG GGGCAGCTGC TGCACCAGCT GCTCTGTGCT
    GGGCATCTGT CTACTGCTCA GTACTACCAA GGGTTGTATG AAATCTTGGA ATTGGCTGAG
    GACATGGAAA TTGACATCCC CCACGTGTGG CTCTACCTAG CGGAACTGGT AACACCCATT
    CTGCAGGAAG GTGGGGTGCC CATGGGGGAG CTGTTCAGGG AGATTACAAA GCCTCTGAGA
    CCGTTGGGCA AAGCTGCTTC CCTGTTGCTG GAGATCCTGG GCCTCCTGTG CAAAAGCATG
    GGTCCTAAAA AGGTGGGGAC GCTGTGGCGA GAAGCCGGGC TTAGCTGGAA GGAATTTCTA
    CCTGAAGGCC AGGACATTGG TGCATTCGTC GCTGAACAGA AGGTGGAGTA TACCCTGGGA
    GAGGAGTCGG AAGCCCCTGG CCAGAGGGCA CTCCCCTCCG AGGAGCTGAA CAGGCAGCTG
    GAGAAGCTGC TGAAGGAGGG CAGCAGTAAC CAGCGGGTGT TCGACTGGAT AGAGGCCAAC
    CTGAGTGAGC AGCAGATAGT ATCCAACACG TTAGTTCGAG CCCTCATGAC GGCTGTCTGC
    TATTCTGCAA TTATTTTTGA GACTCCCCTC CGAGTGGACG TTGCAGTGCT GAAAGCGCGA
    GCGAAGCTGC TGCAGAAATA CCTGTGTGAC GAGCAGAAGG AGCTACAGGC GCTCTACGCC
    CTCCAGGCCC TTGTAGTGAC CTTAGAACAG CCTCCCAACC TGCTGCGGAT GTTCTTTGAC
    GCACTGTATG ACGAGGACGT GGTGAAGGAG GATGCCTTCT ACAGTTGGGA GAGTAGCAAG
    GACCCCGCTG AGCAGCAGGG CAAGGGTGTG GCCCTTAAAT CTGTCACAGC CTTCTTCAAG
    TGGCTCCGTG AAGCAGAGGA GGAGTCTGAC CACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_937887.2
    CDS184..4722
    Translation

    Target ORF information:

    RefSeq Version NM_198244.3
    Organism Homo sapiens(human)
    Definition Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_198244.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATGATCC CTTCCCAGAT CTCCTACCCA GCCTCCCAGG GGGCCTACTA CATCCCTGGA 
    CAGGGGCGTT CCACATACGT TGTCCCGACA CAGCAGTACC CTGTGCAGCC AGGAGCCCCA
    GGCTTCTATC CAGGTGCAAG CCCTACAGAA TTTGGGACCT ACGCTGGCGC CTACTATCCA
    GCCCAAGGGG TGCAGCAGTT TCCCACTGGC GTGGCCCCCA CCCCAGTTTT GATGAACCAG
    CCACCCCAGA TTGCTCCCAA GAGGGAGCGT AAGACGATCC GAATTCGAGA TCCAAACCAA
    GGAGGAAAGG ATATCACAGA GGAGATCATG TCTGGGGCCC GCACTGCCTC CACACCCACC
    CCTCCCCAGA CGGGAGGCGG TCTGGAGCCT CAAGCTAATG GGGAGACGCC CCAGGTTGCT
    GTCATTGTCC GGCCAGATGA CCGGTCACAG GGAGCAATCA TTGCTGACCG GCCAGGGCTG
    CCTGGCCCAG AGCATAGCCC TTCAGAATCC CAGCCTTCGT CGCCTTCTCC GACCCCATCA
    CCATCCCCAG TCTTGGAACC GGGGTCTGAG CCTAATCTCG CAGTCCTCTC TATTCCTGGG
    GACACTATGA CAACTATACA AATGTCTGTA GAAGAATCAA CCCCCATCTC CCGTGAAACT
    GGGGAGCCAT ATCGCCTCTC TCCAGAACCC ACTCCTCTCG CCGAACCCAT ACTGGAAGTA
    GAAGTGACAC TTAGCAAACC GGTTCCAGAA TCTGAGTTTT CTTCCAGTCC TCTCCAGGCT
    CCCACCCCTT TGGCATCTCA CACAGTGGAA ATTCATGAGC CTAATGGCAT GGTCCCATCT
    GAAGATCTGG AACCAGAGGT GGAGTCAAGC CCAGAGCTTG CTCCTCCCCC AGCTTGCCCC
    TCCGAATCCC CTGTGCCCAT TGCTCCAACT GCCCAACCTG AGGAACTGCT CAACGGAGCC
    CCCTCGCCAC CAGCTGTGGA CTTAAGCCCA GTCAGTGAGC CAGAGGAGCA GGCCAAGGAG
    GTGACAGCAT CAATGGCGCC CCCCACCATC CCCTCTGCTA CTCCAGCTAC GGCTCCTTCA
    GCTACTTCCC CAGCTCAGGA GGAGGAAATG GAAGAAGAAG AAGAAGAGGA AGAAGGAGAA
    GCAGGAGAAG CAGGAGAAGC TGAGAGTGAG AAAGGAGGAG AGGAACTGCT CCCCCCAGAG
    AGTACCCCTA TTCCAGCCAA CTTGTCTCAG AATTTGGAGG CAGCAGCAGC CACTCAAGTG
    GCAGTATCTG TGCCAAAGAG GAGACGGAAA ATTAAGGAGC TAAATAAGAA GGAGGCTGTT
    GGAGACCTTC TGGATGCCTT CAAGGAGGCG AACCCGGCAG TACCAGAGGT GGAAAATCAG
    CCTCCTGCAG GCAGCAATCC AGGCCCAGAG TCTGAGGGCA GTGGTGTGCC CCCACGTCCT
    GAGGAAGCAG ATGAGACCTG GGACTCAAAG GAAGACAAAA TTCACAATGC TGAGAACATC
    CAGCCCGGGG AACAGAAGTA TGAATATAAG TCAGATCAGT GGAAGCCTCT AAACCTAGAG
    GAGAAAAAAC GTTACGACCG TGAGTTCCTG CTTGGTTTTC AGTTCATCTT TGCCAGTATG
    CAGAAGCCAG AGGGATTGCC ACATATCAGT GACGTGGTGC TGGACAAGGC CAATAAAACA
    CCACTGCGGC CACTGGATCC CACTAGACTA CAAGGCATAA ATTGTGGCCC AGACTTCACT
    CCATCCTTTG CCAACCTTGG CCGGACAACC CTTAGCACCC GTGGGCCCCC AAGGGGTGGG
    CCAGGTGGGG AGCTGCCCCG TGGGCCGGCT GGCCTGGGAC CCCGGCGCTC TCAGCAGGGA
    CCCCGAAAAG AACCACGCAA GATCATTGCC ACAGTGTTAA TGACCGAAGA TATAAAACTG
    AACAAAGCAG AGAAAGCCTG GAAACCCAGC AGCAAGCGGA CGGCGGCTGA TAAGGATCGA
    GGGGAAGAAG ATGCTGATGG CAGCAAAACC CAGGACCTAT TCCGCAGGGT GCGCTCCATC
    CTGAATAAAC TGACACCCCA GATGTTCCAG CAGCTGATGA AGCAAGTGAC GCAGCTGGCC
    ATCGACACCG AGGAACGCCT CAAAGGGGTC ATTGACCTCA TTTTTGAGAA GGCCATTTCA
    GAGCCCAACT TCTCTGTGGC CTATGCCAAC ATGTGCCGCT GCCTCATGGC GCTGAAAGTG
    CCCACTACGG AAAAGCCAAC AGTGACTGTG AACTTCCGAA AGCTGTTGTT GAATCGATGT
    CAGAAGGAGT TTGAGAAAGA CAAAGATGAT GATGAGGTTT TTGAGAAGAA GCAAAAAGAG
    ATGGATGAAG CTGCTACGGC AGAGGAACGA GGACGCCTGA AGGAAGAGCT GGAAGAGGCT
    CGGGACATAG CCCGGCGGCG CTCTTTAGGG AATATCAAGT TTATTGGAGA GTTGTTCAAA
    CTGAAGATGT TAACAGAGGC AATAATGCAT GACTGTGTGG TCAAACTGCT TAAGAACCAT
    GATGAAGAGT CCCTTGAGTG CCTTTGTCGT CTGCTCACCA CCATTGGCAA AGACCTGGAC
    TTTGAAAAAG CCAAGCCCCG AATGGATCAG TATTTCAACC AGATGGAAAA AATCATTAAA
    GAAAAGAAGA CGTCATCCCG CATCCGCTTT ATGCTGCAGG ACGTGCTGGA TCTGCGAGGG
    AGCAATTGGG TGCCACGCCG AGGGGATCAG GGTCCCAAGA CCATTGACCA GATCCATAAG
    GAGGCTGAGA TGGAAGAACA TCGAGAGCAC ATCAAAGTGC AGCAGCTCAT GGCCAAGGGC
    AGTGACAAGC GTCGGGGCGG TCCTCCAGGC CCTCCCATCA GCCGTGGACT TCCCCTTGTG
    GATGATGGTG GCTGGAACAC AGTTCCCATC AGCAAAGGTA GCCGCCCCAT TGACACCTCA
    CGACTCACCA AGATCACCAA GCCTGGCTCC ATCGATTCTA ACAACCAGCT CTTTGCACCT
    GGAGGGCGAC TGAGCTGGGG CAAGGGCAGC AGCGGAGGCT CAGGAGCCAA GCCCTCAGAC
    GCAGCATCAG AAGCTGCTCG CCCAGCTACT AGTACTTTGA ATCGCTTCTC AGCCCTTCAA
    CAAGCGGTAC CCACAGAAAG CACAGATAAT AGACGTGTGG TGCAGAGGAG TAGCTTGAGC
    CGAGAACGAG GCGAGAAAGC TGGAGACCGA GGAGACCGCC TAGAGCGGAG TGAACGGGGA
    GGGGACCGTG GGGACCGGCT TGATCGTGCG CGGACACCTG CTACCAAGCG GAGCTTCAGC
    AAGGAAGTGG AGGAGCGGAG TAGAGAACGG CCCTCCCAGC CTGAGGGGCT GCGCAAGGCA
    GCTAGCCTCA CGGAGGATCG GGACCGTGGG CGGGATGCCG TGAAGCGAGA AGCTGCCCTA
    CCCCCAGTGA GCCCCCTGAA GGCGGCTCTC TCTGAGGAGG AGTTAGAGAA GAAATCCAAG
    GCTATCATTG AGGAATATCT CCATCTCAAT GACATGAAAG AGGCAGTCCA GTGCGTGCAG
    GAGCTGGCCT CACCCTCCTT GCTCTTCATC TTTGTACGGC ATGGTGTCGA GTCTACGCTG
    GAGCGCAGTG CCATTGCTCG TGAGCATATG GGGCAGCTGC TGCACCAGCT GCTCTGTGCT
    GGGCATCTGT CTACTGCTCA GTACTACCAA GGGTTGTATG AAATCTTGGA ATTGGCTGAG
    GACATGGAAA TTGACATCCC CCACGTGTGG CTCTACCTAG CGGAACTGGT AACACCCATT
    CTGCAGGAAG GTGGGGTGCC CATGGGGGAG CTGTTCAGGG AGATTACAAA GCCTCTGAGA
    CCGTTGGGCA AAGCTGCTTC CCTGTTGCTG GAGATCCTGG GCCTCCTGTG CAAAAGCATG
    GGTCCTAAAA AGGTGGGGAC GCTGTGGCGA GAAGCCGGGC TTAGCTGGAA GGAATTTCTA
    CCTGAAGGCC AGGACATTGG TGCATTCGTC GCTGAACAGA AGGTGGAGTA TACCCTGGGA
    GAGGAGTCGG AAGCCCCTGG CCAGAGGGCA CTCCCCTCCG AGGAGCTGAA CAGGCAGCTG
    GAGAAGCTGC TGAAGGAGGG CAGCAGTAAC CAGCGGGTGT TCGACTGGAT AGAGGCCAAC
    CTGAGTGAGC AGCAGATAGT ATCCAACACG TTAGTTCGAG CCCTCATGAC GGCTGTCTGC
    TATTCTGCAA TTATTTTTGA GACTCCCCTC CGAGTGGACG TTGCAGTGCT GAAAGCGCGA
    GCGAAGCTGC TGCAGAAATA CCTGTGTGAC GAGCAGAAGG AGCTACAGGC GCTCTACGCC
    CTCCAGGCCC TTGTAGTGAC CTTAGAACAG CCTCCCAACC TGCTGCGGAT GTTCTTTGAC
    GCACTGTATG ACGAGGACGT GGTGAAGGAG GATGCCTTCT ACAGTTGGGA GAGTAGCAAG
    GACCCCGCTG AGCAGCAGGG CAAGGGTGTG GCCCTTAAAT CTGTCACAGC CTTCTTCAAG
    TGGCTCCGTG AAGCAGAGGA GGAGTCTGAC CACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13778
    Clone ID Related Accession (Same CDS sequence) NM_198244.2
    Accession Version NM_198244.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4539bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR980540.1, AK299763.1, BC010688.1, BC007788.2 and BQ011010.1. On Aug 13, 2010 this sequence version replaced NM_198244.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, uses a downstream start codon, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (2) is shorter at the N-terminus and lacks an internal residue, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK299763.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATGATCC CTTCCCAGAT CTCCTACCCA GCCTCCCAGG GGGCCTACTA CATCCCTGGA 
    CAGGGGCGTT CCACATACGT TGTCCCGACA CAGCAGTACC CTGTGCAGCC AGGAGCCCCA
    GGCTTCTATC CAGGTGCAAG CCCTACAGAA TTTGGGACCT ACGCTGGCGC CTACTATCCA
    GCCCAAGGGG TGCAGCAGTT TCCCACTGGC GTGGCCCCCG CCCCAGTTTT GATGAACCAG
    CCACCCCAGA TTGCTCCCAA GAGGGAGCGT AAGACGATCC GAATTCGAGA TCCAAACCAA
    GGAGGAAAGG ATATCACAGA GGAGATCATG TCTGGGGCCC GCACTGCCTC CACACCCACC
    CCTCCCCAGA CGGGAGGCGG TCTGGAGCCT CAAGCTAATG GGGAGACGCC CCAGGTTGCT
    GTCATTGTCC GGCCAGATGA CCGGTCACAG GGAGCAATCA TTGCTGACCG GCCAGGGCTG
    CCTGGCCCAG AGCATAGCCC TTCAGAATCC CAGCCTTCGT CGCCTTCTCC GACCCCATCA
    CCATCCCCAG TCTTGGAACC GGGGTCTGAG CCTAATCTCG CAGTCCTCTC TATTCCTGGG
    GACACTATGA CAACTATACA AATGTCTGTA GAAGAATCAA CCCCCATCTC CCGTGAAACT
    GGGGAGCCAT ATCGCCTCTC TCCAGAACCC ACTCCTCTCG CCGAACCCAT ACTGGAAGTA
    GAAGTGACAC TTAGCAAACC GGTTCCAGAA TCTGAGTTTT CTTCCAGTCC TCTCCAGGCT
    CCCACCCCTT TGGCATCTCA CACAGTGGAA ATTCATGAGC CTAATGGCAT GGTCCCATCT
    GAAGATCTGG AACCAGAGGT GGAGTCAAGC CCAGAGCTTG CTCCTCCCCC AGCTTGCCCC
    TCCGAATCCC CTGTGCCCAT TGCTCCAACT GCCCAACCTG AGGAACTGCT CAACGGAGCC
    CCCTCGCCAC CAGCTGTGGA CTTAAGCCCA GTCAGTGAGC CAGAGGAGCA GGCCAAGGAG
    GTGACAGCAT CAATGGCGCC CCCCACCATC CCCTCTGCTA CTCCAGCTAC GGCTCCTTCA
    GCTACTTCCC CAGCTCAGGA GGAGGAAATG GAAGAAGAAG AAGAAGAGGA AGAAGGAGAA
    GCAGGAGAAG CAGGAGAAGC TGAGAGTGAG AAAGGAGGAG AGGAACTGCT CCCCCCAGAG
    AGTACCCCTA TTCCAGCCAA CTTGTCTCAG AATTTGGAGG CAGCAGCAGC CACTCAAGTG
    GCAGTATCTG TGCCAAAGAG GAGACGGAAA ATTAAGGAGC TAAATAAGAA GGAGGCTGTT
    GGAGACCTTC TGGATGCCTT CAAGGAGGCG AACCCGGCAG TACCAGAGGT GGAAAATCAG
    CCTCCTGCAG GCAGCAATCC AGGCCCAGAG TCTGAGGGCA GTGGTGTGCC CCCACGTCCT
    GAGGAAGCAG ATGAGACCTG GGACTCAAAG GAAGACAAAA TTCACAATGC TGAGAACATC
    CAGCCCGGGG AACAGAAGTA TGAATATAAG TCAGATCAGT GGAAGCCTCT AAACCTAGAG
    GAGAAAAAAC GTTACGACCG TGAGTTCCTG CTTGGTTTTC AGTTCATCTT TGCCAGTATG
    CAGAAGCCAG AGGGATTGCC ACATATCAGT GACGTGGTGC TGGACAAGGC CAATAAAACA
    CCACTGCGGC CACTGGATCC CACTAGACTA CAAGGCATAA ATTGTGGCCC AGACTTCACT
    CCATCCTTTG CCAACCTTGG CCGGACAACC CTTAGCACCC GTGGGCCCCC AAGGGGTGGG
    CCAGGTGGGG AGCTGCCCCG TGGGCCGGCT GGCCTGGGAC CCCGGCGCTC TCAGCAGGGA
    CCCCGAAAAG AACCACGCAA GATCATTGCC ACAGTGTTAA TGACCGAAGA TATAAAACTG
    AACAAAGCAG AGAAAGCCTG GAAACCCAGC AGCAAGCGGA CGGCGGCTGA TAAGGATCGA
    GGGGAAGAAG ATGCTGATGG CAGCAAAACC CAGGACCTAT TCCGCAGGGT GCGCTCCATC
    CTGAATAAAC TGACACCCCA GATGTTCCAG CAGCTGATGA AGCAAGTGAC GCAGCTGGCC
    ATCGACACCG AGGAACGCCT CAAAGGGGTC ATTGACCTCA TTTTTGAGAA GGCCATTTCA
    GAGCCCAACT TCTCTGTGGC CTATGCCAAC ATGTGCCGCT GCCTCATGGC GCTGAAAGTG
    CCCACTACGG AAAAGCCAAC AGTGACTGTG AACTTCCGAA AGCTGTTGTT GAATCGATGT
    CAGAAGGAGT TTGAGAAAGA CAAAGATGAT GATGAGGTTT TTGAGAAGAA GCAAAAAGAG
    ATGGATGAAG CTGCTACGGC AGAGGAACGA GGACGCCTGA AGGAAGAGCT GGAAGAGGCT
    CGGGACATAG CCCGGCGGCG CTCTTTAGGG AATATCAAGT TTATTGGAGA GTTGTTCAAA
    CTGAAGATGT TAACAGAGGC AATAATGCAT GACTGTGTGG TCAAACTGCT TAAGAACCAT
    GATGAAGAGT CCCTTGAGTG CCTTTGTCGT CTGCTCACCA CCATTGGCAA AGACCTGGAC
    TTTGAAAAAG CCAAGCCCCG AATGGATCAG TATTTCAACC AGATGGAAAA AATCATTAAA
    GAAAAGAAGA CGTCATCCCG CATCCGCTTT ATGCTGCAGG ACGTGCTGGA TCTGCGAGGG
    AGCAATTGGG TGCCACGCCG AGGGGATCAG GGTCCCAAGA CCATTGACCA GATCCATAAG
    GAGGCTGAGA TGGAAGAACA TCGAGAGCAC ATCAAAGTGC AGCAGCTCAT GGCCAAGGGC
    AGTGACAAGC GTCGGGGCGG TCCTCCAGGC CCTCCCATCA GCCGTGGACT TCCCCTTGTG
    GATGATGGTG GCTGGAACAC AGTTCCCATC AGCAAAGGTA GCCGCCCCAT TGACACCTCA
    CGACTCACCA AGATCACCAA GCCTGGCTCC ATCGATTCTA ACAACCAGCT CTTTGCACCT
    GGAGGGCGAC TGAGCTGGGG CAAGGGCAGC AGCGGAGGCT CAGGAGCCAA GCCCTCAGAC
    GCAGCATCAG AAGCTGCTCG CCCAGCTACT AGTACTTTGA ATCGCTTCTC AGCCCTTCAA
    CAAGCGGTAC CCACAGAAAG CACAGATAAT AGACGTGTGG TGCAGAGGAG TAGCTTGAGC
    CGAGAACGAG GCGAGAAAGC TGGAGACCGA GGAGACCGCC TAGAGCGGAG TGAACGGGGA
    GGGGACCGTG GGGACCGGCT TGATCGTGCG CGGACACCTG CTACCAAGCG GAGCTTCAGC
    AAGGAAGTGG AGGAGCGGAG TAGAGAACGG CCCTCCCAGC CTGAGGGGCT GCGCAAGGCA
    GCTAGCCTCA CGGAGGATCG GGACCGTGGG CGGGATGCCG TGAAGCGAGA AGCTGCCCTA
    CCCCCAGTGA GCCCCCTGAA GGCGGCTCTC TCTGAGGAGG AGTTAGAGAA GAAATCCAAG
    GCTATCATTG AGGAATATCT CCATCTCAAT GACATGAAAG AGGCAGTCCA GTGCGTGCAG
    GAGCTGGCCT CACCCTCCTT GCTCTTCATC TTTGTACGGC ATGGTGTCGA GTCTACGCTG
    GAGCGCAGTG CCATTGCTCG TGAGCATATG GGGCAGCTGC TGCACCAGCT GCTCTGTGCT
    GGGCATCTGT CTACTGCTCA GTACTACCAA GGGTTGTATG AAATCTTGGA ATTGGCTGAG
    GACATGGAAA TTGACATCCC CCACGTGTGG CTCTACCTAG CGGAACTGGT AACACCCATT
    CTGCAGGAAG GTGGGGTGCC CATGGGGGAG CTGTTCAGGG AGATTACAAA GCCTCTGAGA
    CCGTTGGGCA AAGCTGCTTC CCTGTTGCTG GAGATCCTGG GCCTCCTGTG CAAAAGCATG
    GGTCCTAAAA AGGTGGGGAC GCTGTGGCGA GAAGCCGGGC TTAGCTGGAA GGAATTTCTA
    CCTGAAGGCC AGGACATTGG TGCATTCGTC GCTGAACAGA AGGTGGAGTA TACCCTGGGA
    GAGGAGTCGG AAGCCCCTGG CCAGAGGGCA CTCCCCTCCG AGGAGCTGAA CAGGCAGCTG
    GAGAAGCTGC TGAAGGAGGG CAGCAGTAAC CAGCGGGTGT TCGACTGGAT AGAGGCCAAC
    CTGAGTGAGC AGCAGATAGT ATCCAACACG TTAGTTCGAG CCCTCATGAC GGCTGTCTGC
    TATTCTGCAA TTATTTTTGA GACTCCCCTC CGAGTGGACG TTGCAGTGCT GAAAGCGCGA
    GCGAAGCTGC TGCAGAAATA CCTGTGTGAC GAGCAGAAGG AGCTACAGGC GCTCTACGCC
    CTCCAGGCCC TTGTAGTGAC CTTAGAACAG CCTCCCAACC TGCTGCGGAT GTTCTTTGAC
    GCACTGTATG ACGAGGACGT GGTGAAGGAG GATGCCTTCT ACAGTTGGGA GAGTAGCAAG
    GACCCCGCTG AGCAGCAGGG CAAGGGTGTG GCCCTTAAAT CTGTCACAGC CTTCTTCAAG
    TGGCTCCGTG AAGCAGAGGA GGAGTCTGAC CACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_937887.1
    CDS295..4833
    Translation

    Target ORF information:

    RefSeq Version NM_198244.2
    Organism Homo sapiens(human)
    Definition Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_198244.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATGATCC CTTCCCAGAT CTCCTACCCA GCCTCCCAGG GGGCCTACTA CATCCCTGGA 
    CAGGGGCGTT CCACATACGT TGTCCCGACA CAGCAGTACC CTGTGCAGCC AGGAGCCCCA
    GGCTTCTATC CAGGTGCAAG CCCTACAGAA TTTGGGACCT ACGCTGGCGC CTACTATCCA
    GCCCAAGGGG TGCAGCAGTT TCCCACTGGC GTGGCCCCCG CCCCAGTTTT GATGAACCAG
    CCACCCCAGA TTGCTCCCAA GAGGGAGCGT AAGACGATCC GAATTCGAGA TCCAAACCAA
    GGAGGAAAGG ATATCACAGA GGAGATCATG TCTGGGGCCC GCACTGCCTC CACACCCACC
    CCTCCCCAGA CGGGAGGCGG TCTGGAGCCT CAAGCTAATG GGGAGACGCC CCAGGTTGCT
    GTCATTGTCC GGCCAGATGA CCGGTCACAG GGAGCAATCA TTGCTGACCG GCCAGGGCTG
    CCTGGCCCAG AGCATAGCCC TTCAGAATCC CAGCCTTCGT CGCCTTCTCC GACCCCATCA
    CCATCCCCAG TCTTGGAACC GGGGTCTGAG CCTAATCTCG CAGTCCTCTC TATTCCTGGG
    GACACTATGA CAACTATACA AATGTCTGTA GAAGAATCAA CCCCCATCTC CCGTGAAACT
    GGGGAGCCAT ATCGCCTCTC TCCAGAACCC ACTCCTCTCG CCGAACCCAT ACTGGAAGTA
    GAAGTGACAC TTAGCAAACC GGTTCCAGAA TCTGAGTTTT CTTCCAGTCC TCTCCAGGCT
    CCCACCCCTT TGGCATCTCA CACAGTGGAA ATTCATGAGC CTAATGGCAT GGTCCCATCT
    GAAGATCTGG AACCAGAGGT GGAGTCAAGC CCAGAGCTTG CTCCTCCCCC AGCTTGCCCC
    TCCGAATCCC CTGTGCCCAT TGCTCCAACT GCCCAACCTG AGGAACTGCT CAACGGAGCC
    CCCTCGCCAC CAGCTGTGGA CTTAAGCCCA GTCAGTGAGC CAGAGGAGCA GGCCAAGGAG
    GTGACAGCAT CAATGGCGCC CCCCACCATC CCCTCTGCTA CTCCAGCTAC GGCTCCTTCA
    GCTACTTCCC CAGCTCAGGA GGAGGAAATG GAAGAAGAAG AAGAAGAGGA AGAAGGAGAA
    GCAGGAGAAG CAGGAGAAGC TGAGAGTGAG AAAGGAGGAG AGGAACTGCT CCCCCCAGAG
    AGTACCCCTA TTCCAGCCAA CTTGTCTCAG AATTTGGAGG CAGCAGCAGC CACTCAAGTG
    GCAGTATCTG TGCCAAAGAG GAGACGGAAA ATTAAGGAGC TAAATAAGAA GGAGGCTGTT
    GGAGACCTTC TGGATGCCTT CAAGGAGGCG AACCCGGCAG TACCAGAGGT GGAAAATCAG
    CCTCCTGCAG GCAGCAATCC AGGCCCAGAG TCTGAGGGCA GTGGTGTGCC CCCACGTCCT
    GAGGAAGCAG ATGAGACCTG GGACTCAAAG GAAGACAAAA TTCACAATGC TGAGAACATC
    CAGCCCGGGG AACAGAAGTA TGAATATAAG TCAGATCAGT GGAAGCCTCT AAACCTAGAG
    GAGAAAAAAC GTTACGACCG TGAGTTCCTG CTTGGTTTTC AGTTCATCTT TGCCAGTATG
    CAGAAGCCAG AGGGATTGCC ACATATCAGT GACGTGGTGC TGGACAAGGC CAATAAAACA
    CCACTGCGGC CACTGGATCC CACTAGACTA CAAGGCATAA ATTGTGGCCC AGACTTCACT
    CCATCCTTTG CCAACCTTGG CCGGACAACC CTTAGCACCC GTGGGCCCCC AAGGGGTGGG
    CCAGGTGGGG AGCTGCCCCG TGGGCCGGCT GGCCTGGGAC CCCGGCGCTC TCAGCAGGGA
    CCCCGAAAAG AACCACGCAA GATCATTGCC ACAGTGTTAA TGACCGAAGA TATAAAACTG
    AACAAAGCAG AGAAAGCCTG GAAACCCAGC AGCAAGCGGA CGGCGGCTGA TAAGGATCGA
    GGGGAAGAAG ATGCTGATGG CAGCAAAACC CAGGACCTAT TCCGCAGGGT GCGCTCCATC
    CTGAATAAAC TGACACCCCA GATGTTCCAG CAGCTGATGA AGCAAGTGAC GCAGCTGGCC
    ATCGACACCG AGGAACGCCT CAAAGGGGTC ATTGACCTCA TTTTTGAGAA GGCCATTTCA
    GAGCCCAACT TCTCTGTGGC CTATGCCAAC ATGTGCCGCT GCCTCATGGC GCTGAAAGTG
    CCCACTACGG AAAAGCCAAC AGTGACTGTG AACTTCCGAA AGCTGTTGTT GAATCGATGT
    CAGAAGGAGT TTGAGAAAGA CAAAGATGAT GATGAGGTTT TTGAGAAGAA GCAAAAAGAG
    ATGGATGAAG CTGCTACGGC AGAGGAACGA GGACGCCTGA AGGAAGAGCT GGAAGAGGCT
    CGGGACATAG CCCGGCGGCG CTCTTTAGGG AATATCAAGT TTATTGGAGA GTTGTTCAAA
    CTGAAGATGT TAACAGAGGC AATAATGCAT GACTGTGTGG TCAAACTGCT TAAGAACCAT
    GATGAAGAGT CCCTTGAGTG CCTTTGTCGT CTGCTCACCA CCATTGGCAA AGACCTGGAC
    TTTGAAAAAG CCAAGCCCCG AATGGATCAG TATTTCAACC AGATGGAAAA AATCATTAAA
    GAAAAGAAGA CGTCATCCCG CATCCGCTTT ATGCTGCAGG ACGTGCTGGA TCTGCGAGGG
    AGCAATTGGG TGCCACGCCG AGGGGATCAG GGTCCCAAGA CCATTGACCA GATCCATAAG
    GAGGCTGAGA TGGAAGAACA TCGAGAGCAC ATCAAAGTGC AGCAGCTCAT GGCCAAGGGC
    AGTGACAAGC GTCGGGGCGG TCCTCCAGGC CCTCCCATCA GCCGTGGACT TCCCCTTGTG
    GATGATGGTG GCTGGAACAC AGTTCCCATC AGCAAAGGTA GCCGCCCCAT TGACACCTCA
    CGACTCACCA AGATCACCAA GCCTGGCTCC ATCGATTCTA ACAACCAGCT CTTTGCACCT
    GGAGGGCGAC TGAGCTGGGG CAAGGGCAGC AGCGGAGGCT CAGGAGCCAA GCCCTCAGAC
    GCAGCATCAG AAGCTGCTCG CCCAGCTACT AGTACTTTGA ATCGCTTCTC AGCCCTTCAA
    CAAGCGGTAC CCACAGAAAG CACAGATAAT AGACGTGTGG TGCAGAGGAG TAGCTTGAGC
    CGAGAACGAG GCGAGAAAGC TGGAGACCGA GGAGACCGCC TAGAGCGGAG TGAACGGGGA
    GGGGACCGTG GGGACCGGCT TGATCGTGCG CGGACACCTG CTACCAAGCG GAGCTTCAGC
    AAGGAAGTGG AGGAGCGGAG TAGAGAACGG CCCTCCCAGC CTGAGGGGCT GCGCAAGGCA
    GCTAGCCTCA CGGAGGATCG GGACCGTGGG CGGGATGCCG TGAAGCGAGA AGCTGCCCTA
    CCCCCAGTGA GCCCCCTGAA GGCGGCTCTC TCTGAGGAGG AGTTAGAGAA GAAATCCAAG
    GCTATCATTG AGGAATATCT CCATCTCAAT GACATGAAAG AGGCAGTCCA GTGCGTGCAG
    GAGCTGGCCT CACCCTCCTT GCTCTTCATC TTTGTACGGC ATGGTGTCGA GTCTACGCTG
    GAGCGCAGTG CCATTGCTCG TGAGCATATG GGGCAGCTGC TGCACCAGCT GCTCTGTGCT
    GGGCATCTGT CTACTGCTCA GTACTACCAA GGGTTGTATG AAATCTTGGA ATTGGCTGAG
    GACATGGAAA TTGACATCCC CCACGTGTGG CTCTACCTAG CGGAACTGGT AACACCCATT
    CTGCAGGAAG GTGGGGTGCC CATGGGGGAG CTGTTCAGGG AGATTACAAA GCCTCTGAGA
    CCGTTGGGCA AAGCTGCTTC CCTGTTGCTG GAGATCCTGG GCCTCCTGTG CAAAAGCATG
    GGTCCTAAAA AGGTGGGGAC GCTGTGGCGA GAAGCCGGGC TTAGCTGGAA GGAATTTCTA
    CCTGAAGGCC AGGACATTGG TGCATTCGTC GCTGAACAGA AGGTGGAGTA TACCCTGGGA
    GAGGAGTCGG AAGCCCCTGG CCAGAGGGCA CTCCCCTCCG AGGAGCTGAA CAGGCAGCTG
    GAGAAGCTGC TGAAGGAGGG CAGCAGTAAC CAGCGGGTGT TCGACTGGAT AGAGGCCAAC
    CTGAGTGAGC AGCAGATAGT ATCCAACACG TTAGTTCGAG CCCTCATGAC GGCTGTCTGC
    TATTCTGCAA TTATTTTTGA GACTCCCCTC CGAGTGGACG TTGCAGTGCT GAAAGCGCGA
    GCGAAGCTGC TGCAGAAATA CCTGTGTGAC GAGCAGAAGG AGCTACAGGC GCTCTACGCC
    CTCCAGGCCC TTGTAGTGAC CTTAGAACAG CCTCCCAACC TGCTGCGGAT GTTCTTTGAC
    GCACTGTATG ACGAGGACGT GGTGAAGGAG GATGCCTTCT ACAGTTGGGA GAGTAGCAAG
    GACCCCGCTG AGCAGCAGGG CAAGGGTGTG GCCCTTAAAT CTGTCACAGC CTTCTTCAAG
    TGGCTCCGTG AAGCAGAGGA GGAGTCTGAC CACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu15300
    Clone ID Related Accession (Same CDS sequence) NM_001291157.1
    Accession Version NM_001291157.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform 7
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC369776.1, AK226160.1 and BG177454.1. Transcript Variant: This variant (8) differs in the 5' UTR, includes an additional internal segment in the 5' region, which results in a downstream start codon, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (7) is shorter at the N-terminus, and lacks an internal residue, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK226160.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC 
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG
    GGGGCCTACT ACATCCCTGG ACAGGGGCGT TCCACATACG TTGTCCCGAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACAGA ATTTGGGACC
    TACGCTGGCG CCTACTATCC AGCCCAAGGG GTGCAGCAGT TTCCCACTGG CGTGGCCCCC
    GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG TAAGACGATC
    CGAATTCGAG ATCCAAACCA AGGAGGAAAG GATATCACAG AGGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC CCCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAAGCTAAT
    GGGGAGACGC CCCAGGTTGC TGTCATTGTC CGGCCAGATG ACCGGTCACA GGGAGCAATC
    ATTGCTGACC GGCCAGGGCT GCCTGGCCCA GAGCATAGCC CTTCAGAATC CCAGCCTTCG
    TCGCCTTCTC CGACCCCATC ACCATCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC
    GCAGTCCTCT CTATTCCTGG GGACACTATG ACAACTATAC AAATGTCTGT AGAAGAATCA
    ACCCCCATCT CCCGTGAAAC TGGGGAGCCA TATCGCCTCT CTCCAGAACC CACTCCTCTC
    GCCGAACCCA TACTGGAAGT AGAAGTGACA CTTAGCAAAC CGGTTCCAGA ATCTGAGTTT
    TCTTCCAGTC CTCTCCAGGC TCCCACCCCT TTGGCATCTC ACACAGTGGA AATTCATGAG
    CCTAATGGCA TGGTCCCATC TGAAGATCTG GAACCAGAGG TGGAGTCAAG CCCAGAGCTT
    GCTCCTCCCC CAGCTTGCCC CTCCGAATCC CCTGTGCCCA TTGCTCCAAC TGCCCAACCT
    GAGGAACTGC TCAACGGAGC CCCCTCGCCA CCAGCTGTGG ACTTAAGCCC AGTCAGTGAG
    CCAGAGGAGC AGGCCAAGGA GGTGACAGCA TCAATGGCGC CCCCCACCAT CCCCTCTGCT
    ACTCCAGCTA CGGCTCCTTC AGCTACTTCC CCAGCTCAGG AGGAGGAAAT GGAAGAAGAA
    GAAGAAGAGG AAGAAGGAGA AGCAGGAGAA GCAGGAGAAG CTGAGAGTGA GAAAGGAGGA
    GAGGAACTGC TCCCCCCAGA GAGTACCCCT ATTCCAGCCA ACTTGTCTCA GAATTTGGAG
    GCAGCAGCAG CCACTCAAGT GGCAGTATCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG
    CTAAATAAGA AGGAGGCTGT TGGAGACCTT CTGGATGCCT TCAAGGAGGC GAACCCGGCA
    GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCAGCAATC CAGGCCCAGA GTCTGAGGGC
    AGTGGTGTGC CCCCACGTCC TGAGGAAGCA GATGAGACCT GGGACTCAAA GGAAGACAAA
    ATTCACAATG CTGAGAACAT CCAGCCCGGG GAACAGAAGT ATGAATATAA GTCAGATCAG
    TGGAAGCCTC TAAACCTAGA GGAGAAAAAA CGTTACGACC GTGAGTTCCT GCTTGGTTTT
    CAGTTCATCT TTGCCAGTAT GCAGAAGCCA GAGGGATTGC CACATATCAG TGACGTGGTG
    CTGGACAAGG CCAATAAAAC ACCACTGCGG CCACTGGATC CCACTAGACT ACAAGGCATA
    AATTGTGGCC CAGACTTCAC TCCATCCTTT GCCAACCTTG GCCGGACAAC CCTTAGCACC
    CGTGGGCCCC CAAGGGGTGG GCCAGGTGGG GAGCTGCCCC GTGGGCCGGC TGGCCTGGGA
    CCCCGGCGCT CTCAGCAGGG ACCCCGAAAA GAACCACGCA AGATCATTGC CACAGTGTTA
    ATGACCGAAG ATATAAAACT GAACAAAGCA GAGAAAGCCT GGAAACCCAG CAGCAAGCGG
    ACGGCGGCTG ATAAGGATCG AGGGGAAGAA GATGCTGATG GCAGCAAAAC CCAGGACCTA
    TTCCGCAGGG TGCGCTCCAT CCTGAATAAA CTGACACCCC AGATGTTCCA GCAGCTGATG
    AAGCAAGTGA CGCAGCTGGC CATCGACACC GAGGAACGCC TCAAAGGGGT CATTGACCTC
    ATTTTTGAGA AGGCCATTTC AGAGCCCAAC TTCTCTGTGG CCTATGCCAA CATGTGCCGC
    TGCCTCATGG CGCTGAAAGT GCCCACTACG GAAAAGCCAA CAGTGACTGT GAACTTCCGA
    AAGCTGTTGT TGAATCGATG TCAGAAGGAG TTTGAGAAAG ACAAAGATGA TGATGAGGTT
    TTTGAGAAGA AGCAAAAAGA GATGGATGAA GCTGCTACGG CAGAGGAACG AGGACGCCTG
    AAGGAAGAGC TGGAAGAGGC TCGGGACATA GCCCGGCGGC GCTCTTTAGG GAATATCAAG
    TTTATTGGAG AGTTGTTCAA ACTGAAGATG TTAACAGAGG CAATAATGCA TGACTGTGTG
    GTCAAACTGC TTAAGAACCA TGATGAAGAG TCCCTTGAGT GCCTTTGTCG TCTGCTCACC
    ACCATTGGCA AAGACCTGGA CTTTGAAAAA GCCAAGCCCC GAATGGATCA GTATTTCAAC
    CAGATGGAAA AAATCATTAA AGAAAAGAAG ACGTCATCCC GCATCCGCTT TATGCTGCAG
    GACGTGCTGG ATCTGCGAGG GAGCAATTGG GTGCCACGCC GAGGGGATCA GGGTCCCAAG
    ACCATTGACC AGATCCATAA GGAGGCTGAG ATGGAAGAAC ATCGAGAGCA CATCAAAGTG
    CAGCAGCTCA TGGCCAAGGG CAGTGACAAG CGTCGGGGCG GTCCTCCAGG CCCTCCCATC
    AGCCGTGGAC TTCCCCTTGT GGATGATGGT GGCTGGAACA CAGTTCCCAT CAGCAAAGGT
    AGCCGCCCCA TTGACACCTC ACGACTCACC AAGATCACCA AGCCTGGCTC CATCGATTCT
    AACAACCAGC TCTTTGCACC TGGAGGGCGA CTGAGCTGGG GCAAGGGCAG CAGCGGAGGC
    TCAGGAGCCA AGCCCTCAGA CGCAGCATCA GAAGCTGCTC GCCCAGCTAC TAGTACTTTG
    AATCGCTTCT CAGCCCTTCA ACAAGCGGTA CCCACAGAAA GCACAGATAA TAGACGTGTG
    GTGCAGAGGA GTAGCTTGAG CCGAGAACGA GGCGAGAAAG CTGGAGACCG AGGAGACCGC
    CTAGAGCGGA GTGAACGGGG AGGGGACCGT GGGGACCGGC TTGATCGTGC GCGGACACCT
    GCTACCAAGC GGAGCTTCAG CAAGGAAGTG GAGGAGCGGA GTAGAGAACG GCCCTCCCAG
    CCTGAGGGGC TGCGCAAGGC AGCTAGCCTC ACGGAGGATC GGGACCGTGG GCGGGATGCC
    GTGAAGCGAG AAGCTGCCCT ACCCCCAGTG AGCCCCCTGA AGGCGGCTCT CTCTGAGGAG
    GAGTTAGAGA AGAAATCCAA GGCTATCATT GAGGAATATC TCCATCTCAA TGACATGAAA
    GAGGCAGTCC AGTGCGTGCA GGAGCTGGCC TCACCCTCCT TGCTCTTCAT CTTTGTACGG
    CATGGTGTCG AGTCTACGCT GGAGCGCAGT GCCATTGCTC GTGAGCATAT GGGGCAGCTG
    CTGCACCAGC TGCTCTGTGC TGGGCATCTG TCTACTGCTC AGTACTACCA AGGGTTGTAT
    GAAATCTTGG AATTGGCTGA GGACATGGAA ATTGACATCC CCCACGTGTG GCTCTACCTA
    GCGGAACTGG TAACACCCAT TCTGCAGGAA GGTGGGGTGC CCATGGGGGA GCTGTTCAGG
    GAGATTACAA AGCCTCTGAG ACCGTTGGGC AAAGCTGCTT CCCTGTTGCT GGAGATCCTG
    GGCCTCCTGT GCAAAAGCAT GGGTCCTAAA AAGGTGGGGA CGCTGTGGCG AGAAGCCGGG
    CTTAGCTGGA AGGAATTTCT ACCTGAAGGC CAGGACATTG GTGCATTCGT CGCTGAACAG
    AAGGTGGAGT ATACCCTGGG AGAGGAGTCG GAAGCCCCTG GCCAGAGGGC ACTCCCCTCC
    GAGGAGCTGA ACAGGCAGCT GGAGAAGCTG CTGAAGGAGG GCAGCAGTAA CCAGCGGGTG
    TTCGACTGGA TAGAGGCCAA CCTGAGTGAG CAGCAGATAG TATCCAACAC GTTAGTTCGA
    GCCCTCATGA CGGCTGTCTG CTATTCTGCA ATTATTTTTG AGACTCCCCT CCGAGTGGAC
    GTTGCAGTGC TGAAAGCGCG AGCGAAGCTG CTGCAGAAAT ACCTGTGTGA CGAGCAGAAG
    GAGCTACAGG CGCTCTACGC CCTCCAGGCC CTTGTAGTGA CCTTAGAACA GCCTCCCAAC
    CTGCTGCGGA TGTTCTTTGA CGCACTGTAT GACGAGGACG TGGTGAAGGA GGATGCCTTC
    TACAGTTGGG AGAGTAGCAA GGACCCCGCT GAGCAGCAGG GCAAGGGTGT GGCCCTTAAA
    TCTGTCACAG CCTTCTTCAA GTGGCTCCGT GAAGCAGAGG AGGAGTCTGA CCACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001278086.1
    CDS445..5124
    Translation

    Target ORF information:

    RefSeq Version NM_001291157.1
    Organism Homo sapiens(human)
    Definition Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001291157.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC 
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG
    GGGGCCTACT ACATCCCTGG ACAGGGGCGT TCCACATACG TTGTCCCGAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACAGA ATTTGGGACC
    TACGCTGGCG CCTACTATCC AGCCCAAGGG GTGCAGCAGT TTCCCACTGG CGTGGCCCCC
    GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG TAAGACGATC
    CGAATTCGAG ATCCAAACCA AGGAGGAAAG GATATCACAG AGGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC CCCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAAGCTAAT
    GGGGAGACGC CCCAGGTTGC TGTCATTGTC CGGCCAGATG ACCGGTCACA GGGAGCAATC
    ATTGCTGACC GGCCAGGGCT GCCTGGCCCA GAGCATAGCC CTTCAGAATC CCAGCCTTCG
    TCGCCTTCTC CGACCCCATC ACCATCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC
    GCAGTCCTCT CTATTCCTGG GGACACTATG ACAACTATAC AAATGTCTGT AGAAGAATCA
    ACCCCCATCT CCCGTGAAAC TGGGGAGCCA TATCGCCTCT CTCCAGAACC CACTCCTCTC
    GCCGAACCCA TACTGGAAGT AGAAGTGACA CTTAGCAAAC CGGTTCCAGA ATCTGAGTTT
    TCTTCCAGTC CTCTCCAGGC TCCCACCCCT TTGGCATCTC ACACAGTGGA AATTCATGAG
    CCTAATGGCA TGGTCCCATC TGAAGATCTG GAACCAGAGG TGGAGTCAAG CCCAGAGCTT
    GCTCCTCCCC CAGCTTGCCC CTCCGAATCC CCTGTGCCCA TTGCTCCAAC TGCCCAACCT
    GAGGAACTGC TCAACGGAGC CCCCTCGCCA CCAGCTGTGG ACTTAAGCCC AGTCAGTGAG
    CCAGAGGAGC AGGCCAAGGA GGTGACAGCA TCAATGGCGC CCCCCACCAT CCCCTCTGCT
    ACTCCAGCTA CGGCTCCTTC AGCTACTTCC CCAGCTCAGG AGGAGGAAAT GGAAGAAGAA
    GAAGAAGAGG AAGAAGGAGA AGCAGGAGAA GCAGGAGAAG CTGAGAGTGA GAAAGGAGGA
    GAGGAACTGC TCCCCCCAGA GAGTACCCCT ATTCCAGCCA ACTTGTCTCA GAATTTGGAG
    GCAGCAGCAG CCACTCAAGT GGCAGTATCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG
    CTAAATAAGA AGGAGGCTGT TGGAGACCTT CTGGATGCCT TCAAGGAGGC GAACCCGGCA
    GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCAGCAATC CAGGCCCAGA GTCTGAGGGC
    AGTGGTGTGC CCCCACGTCC TGAGGAAGCA GATGAGACCT GGGACTCAAA GGAAGACAAA
    ATTCACAATG CTGAGAACAT CCAGCCCGGG GAACAGAAGT ATGAATATAA GTCAGATCAG
    TGGAAGCCTC TAAACCTAGA GGAGAAAAAA CGTTACGACC GTGAGTTCCT GCTTGGTTTT
    CAGTTCATCT TTGCCAGTAT GCAGAAGCCA GAGGGATTGC CACATATCAG TGACGTGGTG
    CTGGACAAGG CCAATAAAAC ACCACTGCGG CCACTGGATC CCACTAGACT ACAAGGCATA
    AATTGTGGCC CAGACTTCAC TCCATCCTTT GCCAACCTTG GCCGGACAAC CCTTAGCACC
    CGTGGGCCCC CAAGGGGTGG GCCAGGTGGG GAGCTGCCCC GTGGGCCGGC TGGCCTGGGA
    CCCCGGCGCT CTCAGCAGGG ACCCCGAAAA GAACCACGCA AGATCATTGC CACAGTGTTA
    ATGACCGAAG ATATAAAACT GAACAAAGCA GAGAAAGCCT GGAAACCCAG CAGCAAGCGG
    ACGGCGGCTG ATAAGGATCG AGGGGAAGAA GATGCTGATG GCAGCAAAAC CCAGGACCTA
    TTCCGCAGGG TGCGCTCCAT CCTGAATAAA CTGACACCCC AGATGTTCCA GCAGCTGATG
    AAGCAAGTGA CGCAGCTGGC CATCGACACC GAGGAACGCC TCAAAGGGGT CATTGACCTC
    ATTTTTGAGA AGGCCATTTC AGAGCCCAAC TTCTCTGTGG CCTATGCCAA CATGTGCCGC
    TGCCTCATGG CGCTGAAAGT GCCCACTACG GAAAAGCCAA CAGTGACTGT GAACTTCCGA
    AAGCTGTTGT TGAATCGATG TCAGAAGGAG TTTGAGAAAG ACAAAGATGA TGATGAGGTT
    TTTGAGAAGA AGCAAAAAGA GATGGATGAA GCTGCTACGG CAGAGGAACG AGGACGCCTG
    AAGGAAGAGC TGGAAGAGGC TCGGGACATA GCCCGGCGGC GCTCTTTAGG GAATATCAAG
    TTTATTGGAG AGTTGTTCAA ACTGAAGATG TTAACAGAGG CAATAATGCA TGACTGTGTG
    GTCAAACTGC TTAAGAACCA TGATGAAGAG TCCCTTGAGT GCCTTTGTCG TCTGCTCACC
    ACCATTGGCA AAGACCTGGA CTTTGAAAAA GCCAAGCCCC GAATGGATCA GTATTTCAAC
    CAGATGGAAA AAATCATTAA AGAAAAGAAG ACGTCATCCC GCATCCGCTT TATGCTGCAG
    GACGTGCTGG ATCTGCGAGG GAGCAATTGG GTGCCACGCC GAGGGGATCA GGGTCCCAAG
    ACCATTGACC AGATCCATAA GGAGGCTGAG ATGGAAGAAC ATCGAGAGCA CATCAAAGTG
    CAGCAGCTCA TGGCCAAGGG CAGTGACAAG CGTCGGGGCG GTCCTCCAGG CCCTCCCATC
    AGCCGTGGAC TTCCCCTTGT GGATGATGGT GGCTGGAACA CAGTTCCCAT CAGCAAAGGT
    AGCCGCCCCA TTGACACCTC ACGACTCACC AAGATCACCA AGCCTGGCTC CATCGATTCT
    AACAACCAGC TCTTTGCACC TGGAGGGCGA CTGAGCTGGG GCAAGGGCAG CAGCGGAGGC
    TCAGGAGCCA AGCCCTCAGA CGCAGCATCA GAAGCTGCTC GCCCAGCTAC TAGTACTTTG
    AATCGCTTCT CAGCCCTTCA ACAAGCGGTA CCCACAGAAA GCACAGATAA TAGACGTGTG
    GTGCAGAGGA GTAGCTTGAG CCGAGAACGA GGCGAGAAAG CTGGAGACCG AGGAGACCGC
    CTAGAGCGGA GTGAACGGGG AGGGGACCGT GGGGACCGGC TTGATCGTGC GCGGACACCT
    GCTACCAAGC GGAGCTTCAG CAAGGAAGTG GAGGAGCGGA GTAGAGAACG GCCCTCCCAG
    CCTGAGGGGC TGCGCAAGGC AGCTAGCCTC ACGGAGGATC GGGACCGTGG GCGGGATGCC
    GTGAAGCGAG AAGCTGCCCT ACCCCCAGTG AGCCCCCTGA AGGCGGCTCT CTCTGAGGAG
    GAGTTAGAGA AGAAATCCAA GGCTATCATT GAGGAATATC TCCATCTCAA TGACATGAAA
    GAGGCAGTCC AGTGCGTGCA GGAGCTGGCC TCACCCTCCT TGCTCTTCAT CTTTGTACGG
    CATGGTGTCG AGTCTACGCT GGAGCGCAGT GCCATTGCTC GTGAGCATAT GGGGCAGCTG
    CTGCACCAGC TGCTCTGTGC TGGGCATCTG TCTACTGCTC AGTACTACCA AGGGTTGTAT
    GAAATCTTGG AATTGGCTGA GGACATGGAA ATTGACATCC CCCACGTGTG GCTCTACCTA
    GCGGAACTGG TAACACCCAT TCTGCAGGAA GGTGGGGTGC CCATGGGGGA GCTGTTCAGG
    GAGATTACAA AGCCTCTGAG ACCGTTGGGC AAAGCTGCTT CCCTGTTGCT GGAGATCCTG
    GGCCTCCTGT GCAAAAGCAT GGGTCCTAAA AAGGTGGGGA CGCTGTGGCG AGAAGCCGGG
    CTTAGCTGGA AGGAATTTCT ACCTGAAGGC CAGGACATTG GTGCATTCGT CGCTGAACAG
    AAGGTGGAGT ATACCCTGGG AGAGGAGTCG GAAGCCCCTG GCCAGAGGGC ACTCCCCTCC
    GAGGAGCTGA ACAGGCAGCT GGAGAAGCTG CTGAAGGAGG GCAGCAGTAA CCAGCGGGTG
    TTCGACTGGA TAGAGGCCAA CCTGAGTGAG CAGCAGATAG TATCCAACAC GTTAGTTCGA
    GCCCTCATGA CGGCTGTCTG CTATTCTGCA ATTATTTTTG AGACTCCCCT CCGAGTGGAC
    GTTGCAGTGC TGAAAGCGCG AGCGAAGCTG CTGCAGAAAT ACCTGTGTGA CGAGCAGAAG
    GAGCTACAGG CGCTCTACGC CCTCCAGGCC CTTGTAGTGA CCTTAGAACA GCCTCCCAAC
    CTGCTGCGGA TGTTCTTTGA CGCACTGTAT GACGAGGACG TGGTGAAGGA GGATGCCTTC
    TACAGTTGGG AGAGTAGCAA GGACCCCGCT GAGCAGCAGG GCAAGGGTGT GGCCCTTAAA
    TCTGTCACAG CCTTCTTCAA GTGGCTCCGT GAAGCAGAGG AGGAGTCTGA CCACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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