×

EN2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol EN2
Entrez Gene ID 2020
Full Name engrailed homeobox 2
General protein information
Preferred Names
homeobox protein engrailed-2
Names
homeobox protein engrailed-2
hu-En-2
engrailed-2
engrailed homolog 2
homeobox protein en-2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

7

7q36

Summary Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

131310

Disorder Html: {Autism susceptibility 10}, 611016 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_001427 NP_001418 homeobox protein engrailed-2


Homo sapiens (human) EN2 NP_001418.2
Pan troglodytes (chimpanzee) EN2 XP_001146034.2
Macaca mulatta (Rhesus monkey) EN2 XP_001106130.1
Mus musculus (house mouse) En2 NP_034264.1
Rattus norvegicus (Norway rat) En2 NP_001102684.1
Gallus gallus (chicken) LOC100859435 NP_001254648.1
Danio rerio (zebrafish) eng2b NP_571115.1
Danio rerio (zebrafish) eng2a NP_571119.2
Xenopus (Silurana) tropicalis (western clawed frog) LOC100498251 XP_002932525.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu17809
NM_001427 Homo sapiens engrailed homeobox 2 (EN2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu17809
Clone ID Related Accession (Same CDS sequence) NM_001427
Accession Version NM_001427.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1002bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product homeobox protein engrailed-2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008060.5 and H16179.1. This sequence is a reference standard in the RefSeqGene project. On Feb 23, 2007 this sequence version replaced gi:7710120. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC104970.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGAGGAGA ATGACCCCAA GCCTGGCGAA GCAGCGGCGG CGGTGGAGGG ACAGCGGCAG 
CCGGAATCCA GCCCCGGCGG CGGCTCGGGC GGCGGCGGCG GTAGCAGCCC GGGCGAAGCG
GACACCGGGC GCCGGCGGGC TCTGATGCTG CCCGCGGTCC TGCAGGCGCC CGGCAACCAC
CAGCACCCGC ACCGCATCAC CAACTTCTTC ATCGACAACA TCCTGCGGCC CGAGTTCGGC
CGGCGAAAGG ACGCGGGGAC CTGCTGTGCG GGCGCGGGAG GAGGAAGGGG CGGCGGAGCC
GGCGGCGAAG GCGGCGCGAG CGGTGCGGAG GGAGGCGGCG GCGCGGGCGG CTCGGAGCAG
CTCTTGGGCT CGGGCTCCCG AGAGCCCCGG CAGAACCCGC CATGTGCGCC CGGCGCGGGC
GGGCCGCTCC CAGCCGCCGG CAGCGACTCT CCGGGTGACG GGGAAGGCGG CTCCAAGACG
CTCTCGCTGC ACGGTGGCGC CAAGAAAGGC GGCGACCCCG GCGGCCCCCT GGACGGGTCG
CTCAAGGCCC GCGGCTTGGG CGGCGGCGAC CTGTCGGTGA GCTCGGACTC GGACAGCTCG
CAAGCCGGCG CCAACCTGGG CGCGCAGCCC ATGCTCTGGC CGGCGTGGGT CTACTGTACG
CGCTACTCGG ACCGGCCTTC TTCAGGTCCC AGGTCTCGAA AACCAAAGAA GAAGAACCCG
AACAAAGAGG ACAAGCGGCC GCGCACGGCC TTTACCGCCG AGCAGCTGCA GAGGCTCAAG
GCCGAGTTCC AGACCAACAG GTACCTGACG GAGCAGCGGC GCCAGAGCCT GGCGCAGGAG
CTGAGCCTCA ACGAGTCACA GATCAAGATT TGGTTCCAGA ACAAGCGCGC CAAGATCAAG
AAGGCCACGG GCAACAAGAA CACGCTGGCC GTGCACCTCA TGGCACAGGG CTTGTACAAC
CACTCCACCA CAGCCAAGGA GGGCAAGTCG GACAGCGAGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001418.2
CDS250..1251
Misc Feature(1)97..99(+)
Misc Feature(2)976..1155(+)
Misc Feature(3)982..1152(+)
Misc Feature(4)982..1152(+)
Misc Feature(5)988..1140(+)
Misc Feature(6)1075..1077(+)
Misc Feature(7)1153..1248(+)
Exon (1)1..934
Gene:EN2
Gene Synonym:
Exon (2)935..3395
Gene:EN2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001427
Organism Homo sapiens (human)
Definition Homo sapiens engrailed homeobox 2 (EN2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001427

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGAGGAGA ATGACCCCAA GCCTGGCGAA GCAGCGGCGG CGGTGGAGGG ACAGCGGCAG 
CCGGAATCCA GCCCCGGCGG CGGCTCGGGC GGCGGCGGCG GTAGCAGCCC GGGCGAAGCG
GACACCGGGC GCCGGCGGGC TCTGATGCTG CCCGCGGTCC TGCAGGCGCC CGGCAACCAC
CAGCACCCGC ACCGCATCAC CAACTTCTTC ATCGACAACA TCCTGCGGCC CGAGTTCGGC
CGGCGAAAGG ACGCGGGGAC CTGCTGTGCG GGCGCGGGAG GAGGAAGGGG CGGCGGAGCC
GGCGGCGAAG GCGGCGCGAG CGGTGCGGAG GGAGGCGGCG GCGCGGGCGG CTCGGAGCAG
CTCTTGGGCT CGGGCTCCCG AGAGCCCCGG CAGAACCCGC CATGTGCGCC CGGCGCGGGC
GGGCCGCTCC CAGCCGCCGG CAGCGACTCT CCGGGTGACG GGGAAGGCGG CTCCAAGACG
CTCTCGCTGC ACGGTGGCGC CAAGAAAGGC GGCGACCCCG GCGGCCCCCT GGACGGGTCG
CTCAAGGCCC GCGGCTTGGG CGGCGGCGAC CTGTCGGTGA GCTCGGACTC GGACAGCTCG
CAAGCCGGCG CCAACCTGGG CGCGCAGCCC ATGCTCTGGC CGGCGTGGGT CTACTGTACG
CGCTACTCGG ACCGGCCTTC TTCAGGTCCC AGGTCTCGAA AACCAAAGAA GAAGAACCCG
AACAAAGAGG ACAAGCGGCC GCGCACGGCC TTTACCGCCG AGCAGCTGCA GAGGCTCAAG
GCCGAGTTCC AGACCAACAG GTACCTGACG GAGCAGCGGC GCCAGAGCCT GGCGCAGGAG
CTGAGCCTCA ACGAGTCACA GATCAAGATT TGGTTCCAGA ACAAGCGCGC CAAGATCAAG
AAGGCCACGG GCAACAAGAA CACGCTGGCC GTGCACCTCA TGGCACAGGG CTTGTACAAC
CACTCCACCA CAGCCAAGGA GGGCAAGTCG GACAGCGAGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Expression of Engrailed-2 (EN2) protein in bladder cancer and its potential utility as a urinary diagnostic biomarker
Eur. J. Cancer 49 (9), 2214-2222 (2013)
Morgan R, Bryan RT, Javed S, Launchbury F, Zeegers MP, Cheng KK, James ND, Wallace DM, Hurst CD, Ward DG, Knowles MA and Pandha H.


book

Role of Engrailed-2 (EN2) as a prostate cancer detection biomarker in genetically high risk men
Sci Rep 3, 2059 (2013)
Killick E, Morgan R, Launchbury F, Bancroft E, Page E, Castro E, Kote-Jarai Z, Aprikian A, Blanco I, Clowes V, Domchek S, Douglas F, Eccles D, Evans DG, Harris M, Kirk J, Lam J, Lindeman G, Mitchell G, Pachter N, Selkirk C, Tucker K, Zgajnar J, Eeles R and Pandha H.


book

Complex epigenetic regulation of engrailed-2 (EN-2) homeobox gene in the autism cerebellum
Transl Psychiatry 3, E232 (2013)
James SJ, Shpyleva S, Melnyk S, Pavliv O and Pogribny IP.


book

The WNT2 gene polymorphism associated with speech delay inherent to autism
Res Dev Disabil 33 (5), 1533-1540 (2012)
Lin PI, Chien YL, Wu YY, Chen CH, Gau SS, Huang YS, Liu SK, Tsai WC and Chiu YN.


book

Cut-like homeobox 1 and nuclear factor I/B mediate ENGRAILED2 autism spectrum disorder-associated haplotype function
Hum. Mol. Genet. 21 (7), 1566-1580 (2012)
Choi J, Ababon MR, Matteson PG and Millonig JH.


book

Deregulated expression of PAX5 in medulloblastoma
Proc. Natl. Acad. Sci. U.S.A. 92 (12), 5709-5713 (1995)
Kozmik Z, Sure U, Ruedi D, Busslinger M and Aguzzi A.


book

Cloning and sequence comparison of the mouse, human, and chicken engrailed genes reveal potential functional domains and regulatory regions
Dev. Genet. 13 (5), 345-358 (1992)
Logan C, Hanks MC, Noble-Topham S, Nallainathan D, Provart NJ and Joyner AL.


book

Subtle cerebellar phenotype in mice homozygous for a targeted deletion of the En-2 homeobox
Science 251 (4998), 1239-1243 (1991)
Joyner AL, Herrup K, Auerbach BA, Davis CA and Rossant J.


book

Isolation and chromosomal localization of the human En-2 gene
Genomics 4 (3), 225-231 (1989)
Poole SJ, Law ML, Kao FT and Lau YF.


book

Chromosomal localization of the human homeo box-containing genes, EN1 and EN2
Genomics 4 (2), 206-209 (1989)
Logan C, Willard HF, Rommens JM and Joyner AL.


 
*
*
*
*