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EN2 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol EN2
Entrez Gene ID 2020
Full Name engrailed homeobox 2
General protein information
Preferred Names

engrailed homeobox 2

Names

homeobox protein engrailed-2
engrailed homolog 2
engrailed-2
homeobox protein en-2
hu-En-2

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

7

7q36.3

Summary Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008].
Disorder MIM:

131310

Disorder Html: {Autism susceptibility 10}, 611016 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_001427.3 NP_001418.2 homeobox protein engrailed-2



Gallus gallus (chicken) LOC100859435 NP_001254648.1
Danio rerio (zebrafish) eng2a NP_571119.2
Xenopus tropicalis (tropical clawed frog) LOC100498251 XP_002932525.1
Pan troglodytes (chimpanzee) EN2 XP_001146034.2
Mus musculus (house mouse) En2 NP_034264.1
Danio rerio (zebrafish) eng2b NP_571115.1
Homo sapiens (human) EN2 NP_001418.2
Rattus norvegicus (Norway rat) En2 NP_001102684.1
Macaca mulatta (rhesus monkey) EN2 XP_001106130.1


Related articles in PubMed

Repression of engrailed 2 inhibits the proliferation and invasion of human bladder cancer in vitro and in vivo.
Li Y, Liu H, Lai C, Su Z, Heng B, Gao S
Oncology reports33(5)2319-30(2015 May)

Engrailed-2 protein as a potential urinary prostate cancer biomarker: a comparison study before and after digital rectal examination.
Marszałł MP, Sroka W, Adamowski M, Słupski P, Jarzemski P, Siódmiak J, Odrowąż-Sypniewska G
European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP)24(1)51-6(2015 Jan)

Elevated 5-hydroxymethylcytosine in the Engrailed-2 (EN-2) promoter is associated with increased gene expression and decreased MeCP2 binding in autism cerebellum.
James SJ, Shpyleva S, Melnyk S, Pavliv O, Pogribny IP
Translational psychiatry4e460(2014 Oct)

Engrailed-2 is down-regulated but also ectopically expressed in clear cell renal cell carcinoma.
Lai CY, Pan B, Luo Y, Liang WB, Chen J, Ye DM, Guo JN, Li L, Su ZX
Molecular biology reports41(6)3651-7(2014 Jun)

Lineage-specific function of Engrailed-2 in the progression of chronic myelogenous leukemia to T-cell blast crisis.
Abollo-Jiménez F, Campos-Sánchez E, Toboso-Navasa A, Vicente-Dueñas C, González-Herrero I, Alonso-Escudero E, González M, Segura V, Blanco O, Martínez-Climent JA, Sánchez-García I, Cobaleda C
Cell cycle (Georgetown, Tex.)13(11)1717-26(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our data suggest that the functional interaction of miR-33a and EN-2 is involved in tumorigenesis of prostate cancer
Title: Biological function and mechanism of miR-33a in prostate cancer survival and metastasis: via downregulating Engrailed-2.

the results demonstrated that EN2 might play an anti-oncogenic role in oncogenesis and development of CCRCC, thereby maintaining the normal growth of human renal tubular epithelial cells.
Title: Engrailed-2 might play an anti-oncogenic role in clear-cell renal cell carcinoma.

EN2 may be a candidate oncogene in bladder cancer by activating the PI3K/Akt pathway and inhibiting PTEN.
Title: Repression of engrailed 2 inhibits the proliferation and invasion of human bladder cancer in vitro and in vivo.

The mean EN2 levels in prostate cancer patients were 3.76-fold higher than those in non-prostate patients after prostate massage.
Title: Engrailed-2 protein as a potential urinary prostate cancer biomarker: a comparison study before and after digital rectal examination.

Engrailed-2 (EN-2) is a developmentally regulated homeobox gene that is essential for Purkinje cell maturation and normal cerebellar development and patterning.
Title: Elevated 5-hydroxymethylcytosine in the Engrailed-2 (EN-2) promoter is associated with increased gene expression and decreased MeCP2 binding in autism cerebellum.

The following EN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu17809
NM_001427.3
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Homo sapiens engrailed homeobox 2 (EN2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu17809
Clone ID Related Accession (Same CDS sequence) NM_001427.3
Accession Version NM_001427.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1002bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-29
Organism Homo sapiens(Human)
Product homeobox protein engrailed-2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008060.5 and H16179.1. This sequence is a reference standard in the RefSeqGene project. On Feb 23, 2007 this sequence version replaced NM_001427.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC104970.1, SRR1660805.106245.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGAGGAGA ATGACCCCAA GCCTGGCGAA GCAGCGGCGG CGGTGGAGGG ACAGCGGCAG 
CCGGAATCCA GCCCCGGCGG CGGCTCGGGC GGCGGCGGCG GTAGCAGCCC GGGCGAAGCG
GACACCGGGC GCCGGCGGGC TCTGATGCTG CCCGCGGTCC TGCAGGCGCC CGGCAACCAC
CAGCACCCGC ACCGCATCAC CAACTTCTTC ATCGACAACA TCCTGCGGCC CGAGTTCGGC
CGGCGAAAGG ACGCGGGGAC CTGCTGTGCG GGCGCGGGAG GAGGAAGGGG CGGCGGAGCC
GGCGGCGAAG GCGGCGCGAG CGGTGCGGAG GGAGGCGGCG GCGCGGGCGG CTCGGAGCAG
CTCTTGGGCT CGGGCTCCCG AGAGCCCCGG CAGAACCCGC CATGTGCGCC CGGCGCGGGC
GGGCCGCTCC CAGCCGCCGG CAGCGACTCT CCGGGTGACG GGGAAGGCGG CTCCAAGACG
CTCTCGCTGC ACGGTGGCGC CAAGAAAGGC GGCGACCCCG GCGGCCCCCT GGACGGGTCG
CTCAAGGCCC GCGGCTTGGG CGGCGGCGAC CTGTCGGTGA GCTCGGACTC GGACAGCTCG
CAAGCCGGCG CCAACCTGGG CGCGCAGCCC ATGCTCTGGC CGGCGTGGGT CTACTGTACG
CGCTACTCGG ACCGGCCTTC TTCAGGTCCC AGGTCTCGAA AACCAAAGAA GAAGAACCCG
AACAAAGAGG ACAAGCGGCC GCGCACGGCC TTTACCGCCG AGCAGCTGCA GAGGCTCAAG
GCCGAGTTCC AGACCAACAG GTACCTGACG GAGCAGCGGC GCCAGAGCCT GGCGCAGGAG
CTGAGCCTCA ACGAGTCACA GATCAAGATT TGGTTCCAGA ACAAGCGCGC CAAGATCAAG
AAGGCCACGG GCAACAAGAA CACGCTGGCC GTGCACCTCA TGGCACAGGG CTTGTACAAC
CACTCCACCA CAGCCAAGGA GGGCAAGTCG GACAGCGAGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001418.2
CDS250..1251
Misc Feature(1)97..99
Misc Feature(2)976..1155
Misc Feature(3)order(982..996,1000..1002,1051..1053,1069..1071,1108..1110,1114..1119,1126..1131,1135..1143,1147..1152)
Misc Feature(4)988..1149
Misc Feature(5)order(988..990,997..999,1117..1119,1126..1131,1138..1140)
Misc Feature(6)1075..1077
Misc Feature(7)1153..1242
Exon (1)1..934
Exon (2)935..3395
Translation

Target ORF information:

RefSeq Version NM_001427.3
Organism Homo sapiens(Human)
Definition Homo sapiens engrailed homeobox 2 (EN2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001427.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGAGGAGA ATGACCCCAA GCCTGGCGAA GCAGCGGCGG CGGTGGAGGG ACAGCGGCAG 
CCGGAATCCA GCCCCGGCGG CGGCTCGGGC GGCGGCGGCG GTAGCAGCCC GGGCGAAGCG
GACACCGGGC GCCGGCGGGC TCTGATGCTG CCCGCGGTCC TGCAGGCGCC CGGCAACCAC
CAGCACCCGC ACCGCATCAC CAACTTCTTC ATCGACAACA TCCTGCGGCC CGAGTTCGGC
CGGCGAAAGG ACGCGGGGAC CTGCTGTGCG GGCGCGGGAG GAGGAAGGGG CGGCGGAGCC
GGCGGCGAAG GCGGCGCGAG CGGTGCGGAG GGAGGCGGCG GCGCGGGCGG CTCGGAGCAG
CTCTTGGGCT CGGGCTCCCG AGAGCCCCGG CAGAACCCGC CATGTGCGCC CGGCGCGGGC
GGGCCGCTCC CAGCCGCCGG CAGCGACTCT CCGGGTGACG GGGAAGGCGG CTCCAAGACG
CTCTCGCTGC ACGGTGGCGC CAAGAAAGGC GGCGACCCCG GCGGCCCCCT GGACGGGTCG
CTCAAGGCCC GCGGCTTGGG CGGCGGCGAC CTGTCGGTGA GCTCGGACTC GGACAGCTCG
CAAGCCGGCG CCAACCTGGG CGCGCAGCCC ATGCTCTGGC CGGCGTGGGT CTACTGTACG
CGCTACTCGG ACCGGCCTTC TTCAGGTCCC AGGTCTCGAA AACCAAAGAA GAAGAACCCG
AACAAAGAGG ACAAGCGGCC GCGCACGGCC TTTACCGCCG AGCAGCTGCA GAGGCTCAAG
GCCGAGTTCC AGACCAACAG GTACCTGACG GAGCAGCGGC GCCAGAGCCT GGCGCAGGAG
CTGAGCCTCA ACGAGTCACA GATCAAGATT TGGTTCCAGA ACAAGCGCGC CAAGATCAAG
AAGGCCACGG GCAACAAGAA CACGCTGGCC GTGCACCTCA TGGCACAGGG CTTGTACAAC
CACTCCACCA CAGCCAAGGA GGGCAAGTCG GACAGCGAGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Repression of engrailed 2 inhibits the proliferation and invasion of human bladder cancer in vitro and in vivo.
Oncology reports33(5)2319-30(2015 May)
Li Y,Liu H,Lai C,Su Z,Heng B,Gao S


book

Engrailed-2 protein as a potential urinary prostate cancer biomarker: a comparison study before and after digital rectal examination.
European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP)24(1)51-6(2015 Jan)
Marszałł MP,Sroka W,Adamowski M,Słupski P,Jarzemski P,Siódmiak J,Odrowąż-Sypniewska G


book

Elevated 5-hydroxymethylcytosine in the Engrailed-2 (EN-2) promoter is associated with increased gene expression and decreased MeCP2 binding in autism cerebellum.
Translational psychiatry4e460(2014 Oct)
James SJ,Shpyleva S,Melnyk S,Pavliv O,Pogribny IP


book

Engrailed-2 is down-regulated but also ectopically expressed in clear cell renal cell carcinoma.
Molecular biology reports41(6)3651-7(2014 Jun)
Lai CY,Pan B,Luo Y,Liang WB,Chen J,Ye DM,Guo JN,Li L,Su ZX


book

Lineage-specific function of Engrailed-2 in the progression of chronic myelogenous leukemia to T-cell blast crisis.
Cell cycle (Georgetown, Tex.)13(11)1717-26(2014)
Abollo-Jiménez F,Campos-Sánchez E,Toboso-Navasa A,Vicente-Dueñas C,González-Herrero I,Alonso-Escudero E,González M,Segura V,Blanco O,Martínez-Climent JA,Sánchez-García I,Cobaleda C


 
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