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EPAS1 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol EPAS1
Entrez Gene ID 2034
Full Name endothelial PAS domain protein 1
Synonyms ECYT4,HIF2A,HLF,MOP2,PASD2,bHLHe73
General protein information
Preferred Names

endothelial PAS domain protein 1

Names

endothelial PAS domain-containing protein 1
EPAS-1
HIF-1-alpha-like factor
HIF-1alpha-like factor
HIF-2-alpha
HIF2-alpha
PAS domain-containing protein 2
basic-helix-loop-helix-PAS protein MOP2
class E basic helix-loop-helix protein 73
hypoxia-inducible factor 2 alpha
member of PAS protein 2
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

2

2p21
Summary This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009].
Disorder MIM:

603349

Disorder Html: Erythrocytosis, familial, 4, 611783 (3)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001430.4 NP_001421.2 endothelial PAS domain-containing protein 1
XM_011532698.2 XP_011531000.1 endothelial PAS domain-containing protein 1 isoform X1

Pathways from BioSystems


Homologies


Canis lupus familiaris (dog) EPAS1 XP_005626137.1
Rattus norvegicus (Norway rat) Epas1 NP_075578.1
EPAS1 XP_001112947.2
Mus musculus (house mouse) Epas1 NP_034267.3
Pan troglodytes (chimpanzee) EPAS1 XP_001147219.1
Gallus gallus (chicken) EPAS1 NP_990138.1
Danio rerio (zebrafish) epas1b NP_001034895.1
Xenopus tropicalis (tropical clawed frog) epas1 NP_001005647.1
Homo sapiens (human) EPAS1 NP_001421.2
Bos taurus (cattle) EPAS1 NP_777150.1

Gene Ontology


Bibliography

Related articles in PubMed

Activation of intestinal hypoxia-inducible factor 2? during obesity contributes to hepatic steatosis.
Xie C, Yagai T, Luo Y, Liang X, Chen T, Wang Q, Sun D, Zhao J, Ramakrishnan SK, Sun L, Jiang C, Xue X, Tian Y, Krausz KW, Patterson AD, Shah YM, Wu Y, Jiang C, Gonzalez FJ
Nature medicine23(11)1298-1308(2017 Nov)

PHGDH as a Key Enzyme for Serine Biosynthesis in HIF2?-Targeting Therapy for Renal Cell Carcinoma.
Yoshino H, Nohata N, Miyamoto K, Yonemori M, Sakaguchi T, Sugita S, Itesako T, Kofuji S, Nakagawa M, Dahiya R, Enokida H
Cancer research77(22)6321-6329(2017 Nov)

Hypoxia-inducible factor 2? (HIF-2?) promotes colon cancer growth by potentiating Yes-associated protein 1 (YAP1) activity.
Ma X, Zhang H, Xue X, Shah YM
The Journal of biological chemistry292(41)17046-17056(2017 10)

Preferential activation of HIF-2? adaptive signalling in neuronal-like cells in response to acute hypoxia.
Mart?n-Arag?n Baudel MAS, Rae MT, Darlison MG, Poole AV, Fraser JA
PloS one12(10)e0185664(2017)

Restoration of the prolyl-hydroxylase domain protein-3 oxygen-sensing mechanism is responsible for regulation of HIF2? expression and induction of sensitivity of myeloma cells to hypoxia-mediated apoptosis.
Gastelum G, Poteshkina A, Veena M, Artiga E, Weckstein G, Frost P
PloS one12(12)e0188438(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show there was a significant negative correlation between PHGDH copy-number alteration and EPAS1 (HIF2A) expression.
Title: PHGDH as a Key Enzyme for Serine Biosynthesis in HIF2?-Targeting Therapy for Renal Cell Carcinoma.

findings indicate that HIF-2alpha increases cancer cell growth by up-regulating YAP1 activity
Title: Hypoxia-inducible factor 2? (HIF-2?) promotes colon cancer growth by potentiating Yes-associated protein 1 (YAP1) activity.

LOX, a hypoxia-responsive gene that encodes lysyl oxidase, is activated by HIF-2-alpha more than HIF-1. Two new hypoxia response elements identified in the LOX promoter mediate most HIF responsiveness.
Title: Identification of functional hypoxia inducible factor response elements in the human lysyl oxidase gene promoter.

Univariate analysis of the overall survival demonstrates that HIF-2a expression but not HIF-1a was related to poor outcome ( p = 0.005) and it retained significance in multivariate analysis ( p = 0.046). In conclusion, HIF-2a expression was related to tumor size, lymph node metastasis, tumor stage and histology. We also found a positive prognostic value of HIF-2a protein expression.
Title: HIF-2? not HIF-1? overexpression confers poor prognosis in non-small cell lung cancer.

findings suggest the HIF-2alpha pathway predominates over HIF-1alpha signalling in neuronal-like cells following acute hypoxia
Title: Preferential activation of HIF-2? adaptive signalling in neuronal-like cells in response to acute hypoxia.

The following EPAS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EPAS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu23001 NM_001430.4
Latest version!		 Homo sapiens endothelial PAS domain protein 1 (EPAS1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $189.50-$265.30
$379.00
OHu57508 XM_011532698.2
Latest version!		 Homo sapiens endothelial PAS domain protein 1 (EPAS1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone 40% OFF
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All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu23001
Clone ID Related Accession (Same CDS sequence) NM_001430.4
Accession Version NM_001430.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2613bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2018-01-01
Organism Homo sapiens(human)
Product endothelial PAS domain-containing protein 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC016696.8, CD109908.1, BC051338.1, AK123845.1 and BM671511.1. This sequence is a reference standard in the RefSeqGene project. On Nov 6, 2009 this sequence version replaced NM_001430.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.67820.1, SRR1660803.153586.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGACAGCTG ACAAGGAGAA GAAAAGGAGT AGCTCGGAGA GGAGGAAGGA GAAGTCCCGG 
GATGCTGCGC GGTGCCGGCG GAGCAAGGAG ACGGAGGTGT TCTATGAGCT GGCCCATGAG 
CTGCCTCTGC CCCACAGTGT GAGCTCCCAT CTGGACAAGG CCTCCATCAT GCGACTGGCA 
ATCAGCTTCC TGCGAACACA CAAGCTCCTC TCCTCAGTTT GCTCTGAAAA CGAGTCCGAA 
GCCGAAGCTG ACCAGCAGAT GGACAACTTG TACCTGAAAG CCTTGGAGGG TTTCATTGCC 
GTGGTGACCC AAGATGGCGA CATGATCTTT CTGTCAGAAA ACATCAGCAA GTTCATGGGA 
CTTACACAGG TGGAGCTAAC AGGACATAGT ATCTTTGACT TCACTCATCC CTGCGACCAT 
GAGGAGATTC GTGAGAACCT GAGTCTCAAA AATGGCTCTG GTTTTGGGAA AAAAAGCAAA 
GACATGTCCA CAGAGCGGGA CTTCTTCATG AGGATGAAGT GCACGGTCAC CAACAGAGGC 
CGTACTGTCA ACCTCAAGTC AGCCACCTGG AAGGTCTTGC ACTGCACGGG CCAGGTGAAA 
GTCTACAACA ACTGCCCTCC TCACAATAGT CTGTGTGGCT ACAAGGAGCC CCTGCTGTCC 
TGCCTCATCA TCATGTGTGA ACCAATCCAG CACCCATCCC ACATGGACAT CCCCCTGGAT 
AGCAAGACCT TCCTGAGCCG CCACAGCATG GACATGAAGT TCACCTACTG TGATGACAGA 
ATCACAGAAC TGATTGGTTA CCACCCTGAG GAGCTGCTTG GCCGCTCAGC CTATGAATTC 
TACCATGCGC TAGACTCCGA GAACATGACC AAGAGTCACC AGAACTTGTG CACCAAGGGT 
CAGGTAGTAA GTGGCCAGTA CCGGATGCTC GCAAAGCATG GGGGCTACGT GTGGCTGGAG 
ACCCAGGGGA CGGTCATCTA CAACCCTCGC AACCTGCAGC CCCAGTGCAT CATGTGTGTC 
AACTACGTCC TGAGTGAGAT TGAGAAGAAT GACGTGGTGT TCTCCATGGA CCAGACTGAA 
TCCCTGTTCA AGCCCCACCT GATGGCCATG AACAGCATCT TTGATAGCAG TGGCAAGGGG 
GCTGTGTCTG AGAAGAGTAA CTTCCTATTC ACCAAGCTAA AGGAGGAGCC CGAGGAGCTG 
GCCCAGCTGG CTCCCACCCC AGGAGACGCC ATCATCTCTC TGGATTTCGG GAATCAGAAC 
TTCGAGGAGT CCTCAGCCTA TGGCAAGGCC ATCCTGCCCC CGAGCCAGCC ATGGGCCACG 
GAGTTGAGGA GCCACAGCAC CCAGAGCGAG GCTGGGAGCC TGCCTGCCTT CACCGTGCCC 
CAGGCAGCTG CCCCGGGCAG CACCACCCCC AGTGCCACCA GCAGCAGCAG CAGCTGCTCC 
ACGCCCAATA GCCCTGAAGA CTATTACACA TCTTTGGATA ACGACCTGAA GATTGAAGTG 
ATTGAGAAGC TCTTCGCCAT GGACACAGAG GCCAAGGACC AATGCAGTAC CCAGACGGAT 
TTCAATGAGC TGGACTTGGA GACACTGGCA CCCTATATCC CCATGGACGG GGAAGACTTC 
CAGCTAAGCC CCATCTGCCC CGAGGAGCGG CTCTTGGCGG AGAACCCACA GTCCACCCCC 
CAGCACTGCT TCAGTGCCAT GACAAACATC TTCCAGCCAC TGGCCCCTGT AGCCCCGCAC 
AGTCCCTTCC TCCTGGACAA GTTTCAGCAG CAGCTGGAGA GCAAGAAGAC AGAGCCCGAG 
CACCGGCCCA TGTCCTCCAT CTTCTTTGAT GCCGGAAGCA AAGCATCCCT GCCACCGTGC 
TGTGGCCAGG CCAGCACCCC TCTCTCTTCC ATGGGGGGCA GATCCAATAC CCAGTGGCCC 
CCAGATCCAC CATTACATTT TGGGCCCACA AAGTGGGCCG TCGGGGATCA GCGCACAGAG 
TTCTTGGGAG CAGCGCCGTT GGGGCCCCCT GTCTCTCCAC CCCATGTCTC CACCTTCAAG 
ACAAGGTCTG CAAAGGGTTT TGGGGCTCGA GGCCCAGACG TGCTGAGTCC GGCCATGGTA 
GCCCTCTCCA ACAAGCTGAA GCTGAAGCGA CAGCTGGAGT ATGAAGAGCA AGCCTTCCAG 
GACCTGAGCG GGGGGGACCC ACCTGGTGGC AGCACCTCAC ATTTGATGTG GAAACGGATG 
AAGAACCTCA GGGGTGGGAG CTGCCCTTTG ATGCCGGACA AGCCACTGAG CGCAAATGTA 
CCCAATGATA AGTTCACCCA AAACCCCATG AGGGGCCTGG GCCATCCCCT GAGACATCTG 
CCGCTGCCAC AGCCTCCATC TGCCATCAGT CCCGGGGAGA ACAGCAAGAG CAGGTTCCCC 
CCACAGTGCT ACGCCACCCA GTACCAGGAC TACAGCCTGT CGTCAGCCCA CAAGGTGTCA 
GGCATGGCAA GCCGGCTGCT CGGGCCCTCA TTTGAGTCCT ACCTGCTGCC CGAACTGACC 
AGATATGACT GTGAGGTGAA CGTGCCCGTG CTGGGAAGCT CCACGCTCCT GCAAGGAGGG 
GACCTCCTCA GAGCCCTGGA CCAGGCCACC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001421.2
CDS511..3123
Misc Feature(1)484..486
Misc Feature(2)544..714
Misc Feature(3)order(553..558,574..582,586..588,658..660,667..669)
Misc Feature(4)577..579
Misc Feature(5)order(595..600,607..612,616..621,670..672,679..681,691..693,697..702,712..714)
Misc Feature(6)766..951
Misc Feature(7)1231..1530
Misc Feature(8)1270..1533
Misc Feature(9)order(1279..1281,1291..1293,1309..1311,1348..1359,1435..1437,1450..1452)
Misc Feature(10)order(1339..1341,1351..1353,1375..1377,1384..1389,1471..1473,1477..1479)
Misc Feature(11)1723..1725
Misc Feature(12)1996..2136
Misc Feature(13)2059..2157
Misc Feature(14)2101..2103
Misc Feature(15)2998..3120
Misc Feature(16)3007..3117
Misc Feature(17)3028..3030
Misc Feature(18)3049..3051
Exon (1)1..536
Exon (2)537..727
Exon (3)728..879
Exon (4)880..964
Exon (5)965..1083
Exon (6)1084..1289
Exon (7)1290..1396
Exon (8)1397..1544
Exon (9)1545..1759
Exon (10)1760..1953
Exon (11)1954..2064
Exon (12)2065..2555
Exon (13)2556..2682
Exon (14)2683..2797
Exon (15)2798..2971
Exon (16)2972..5166
Translation

Target ORF information:

RefSeq Version NM_001430.4
Organism Homo sapiens(human)
Definition Homo sapiens endothelial PAS domain protein 1 (EPAS1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001430.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGACAGCTG ACAAGGAGAA GAAAAGGAGT AGCTCGGAGA GGAGGAAGGA GAAGTCCCGG 
GATGCTGCGC GGTGCCGGCG GAGCAAGGAG ACGGAGGTGT TCTATGAGCT GGCCCATGAG 
CTGCCTCTGC CCCACAGTGT GAGCTCCCAT CTGGACAAGG CCTCCATCAT GCGACTGGCA 
ATCAGCTTCC TGCGAACACA CAAGCTCCTC TCCTCAGTTT GCTCTGAAAA CGAGTCCGAA 
GCCGAAGCTG ACCAGCAGAT GGACAACTTG TACCTGAAAG CCTTGGAGGG TTTCATTGCC 
GTGGTGACCC AAGATGGCGA CATGATCTTT CTGTCAGAAA ACATCAGCAA GTTCATGGGA 
CTTACACAGG TGGAGCTAAC AGGACATAGT ATCTTTGACT TCACTCATCC CTGCGACCAT 
GAGGAGATTC GTGAGAACCT GAGTCTCAAA AATGGCTCTG GTTTTGGGAA AAAAAGCAAA 
GACATGTCCA CAGAGCGGGA CTTCTTCATG AGGATGAAGT GCACGGTCAC CAACAGAGGC 
CGTACTGTCA ACCTCAAGTC AGCCACCTGG AAGGTCTTGC ACTGCACGGG CCAGGTGAAA 
GTCTACAACA ACTGCCCTCC TCACAATAGT CTGTGTGGCT ACAAGGAGCC CCTGCTGTCC 
TGCCTCATCA TCATGTGTGA ACCAATCCAG CACCCATCCC ACATGGACAT CCCCCTGGAT 
AGCAAGACCT TCCTGAGCCG CCACAGCATG GACATGAAGT TCACCTACTG TGATGACAGA 
ATCACAGAAC TGATTGGTTA CCACCCTGAG GAGCTGCTTG GCCGCTCAGC CTATGAATTC 
TACCATGCGC TAGACTCCGA GAACATGACC AAGAGTCACC AGAACTTGTG CACCAAGGGT 
CAGGTAGTAA GTGGCCAGTA CCGGATGCTC GCAAAGCATG GGGGCTACGT GTGGCTGGAG 
ACCCAGGGGA CGGTCATCTA CAACCCTCGC AACCTGCAGC CCCAGTGCAT CATGTGTGTC 
AACTACGTCC TGAGTGAGAT TGAGAAGAAT GACGTGGTGT TCTCCATGGA CCAGACTGAA 
TCCCTGTTCA AGCCCCACCT GATGGCCATG AACAGCATCT TTGATAGCAG TGGCAAGGGG 
GCTGTGTCTG AGAAGAGTAA CTTCCTATTC ACCAAGCTAA AGGAGGAGCC CGAGGAGCTG 
GCCCAGCTGG CTCCCACCCC AGGAGACGCC ATCATCTCTC TGGATTTCGG GAATCAGAAC 
TTCGAGGAGT CCTCAGCCTA TGGCAAGGCC ATCCTGCCCC CGAGCCAGCC ATGGGCCACG 
GAGTTGAGGA GCCACAGCAC CCAGAGCGAG GCTGGGAGCC TGCCTGCCTT CACCGTGCCC 
CAGGCAGCTG CCCCGGGCAG CACCACCCCC AGTGCCACCA GCAGCAGCAG CAGCTGCTCC 
ACGCCCAATA GCCCTGAAGA CTATTACACA TCTTTGGATA ACGACCTGAA GATTGAAGTG 
ATTGAGAAGC TCTTCGCCAT GGACACAGAG GCCAAGGACC AATGCAGTAC CCAGACGGAT 
TTCAATGAGC TGGACTTGGA GACACTGGCA CCCTATATCC CCATGGACGG GGAAGACTTC 
CAGCTAAGCC CCATCTGCCC CGAGGAGCGG CTCTTGGCGG AGAACCCACA GTCCACCCCC 
CAGCACTGCT TCAGTGCCAT GACAAACATC TTCCAGCCAC TGGCCCCTGT AGCCCCGCAC 
AGTCCCTTCC TCCTGGACAA GTTTCAGCAG CAGCTGGAGA GCAAGAAGAC AGAGCCCGAG 
CACCGGCCCA TGTCCTCCAT CTTCTTTGAT GCCGGAAGCA AAGCATCCCT GCCACCGTGC 
TGTGGCCAGG CCAGCACCCC TCTCTCTTCC ATGGGGGGCA GATCCAATAC CCAGTGGCCC 
CCAGATCCAC CATTACATTT TGGGCCCACA AAGTGGGCCG TCGGGGATCA GCGCACAGAG 
TTCTTGGGAG CAGCGCCGTT GGGGCCCCCT GTCTCTCCAC CCCATGTCTC CACCTTCAAG 
ACAAGGTCTG CAAAGGGTTT TGGGGCTCGA GGCCCAGACG TGCTGAGTCC GGCCATGGTA 
GCCCTCTCCA ACAAGCTGAA GCTGAAGCGA CAGCTGGAGT ATGAAGAGCA AGCCTTCCAG 
GACCTGAGCG GGGGGGACCC ACCTGGTGGC AGCACCTCAC ATTTGATGTG GAAACGGATG 
AAGAACCTCA GGGGTGGGAG CTGCCCTTTG ATGCCGGACA AGCCACTGAG CGCAAATGTA 
CCCAATGATA AGTTCACCCA AAACCCCATG AGGGGCCTGG GCCATCCCCT GAGACATCTG 
CCGCTGCCAC AGCCTCCATC TGCCATCAGT CCCGGGGAGA ACAGCAAGAG CAGGTTCCCC 
CCACAGTGCT ACGCCACCCA GTACCAGGAC TACAGCCTGT CGTCAGCCCA CAAGGTGTCA 
GGCATGGCAA GCCGGCTGCT CGGGCCCTCA TTTGAGTCCT ACCTGCTGCC CGAACTGACC 
AGATATGACT GTGAGGTGAA CGTGCCCGTG CTGGGAAGCT CCACGCTCCT GCAAGGAGGG 
GACCTCCTCA GAGCCCTGGA CCAGGCCACC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57508
Clone ID Related Accession (Same CDS sequence) XM_011532698.2
Accession Version XM_011532698.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2652bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product endothelial PAS domain-containing protein 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532698.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
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1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGTTAGGGC TTTTTGTTTT TAAAACAGGG ACACAAAGAA GAAACAGAAG GCTGCCATAT 
GCAAGGAGTA GCTCGGAGAG GAGGAAGGAG AAGTCCCGGG ATGCTGCGCG GTGCCGGCGG 
AGCAAGGAGA CGGAGGTGTT CTATGAGCTG GCCCATGAGC TGCCTCTGCC CCACAGTGTG 
AGCTCCCATC TGGACAAGGC CTCCATCATG CGACTGGCAA TCAGCTTCCT GCGAACACAC 
AAGCTCCTCT CCTCAGTTTG CTCTGAAAAC GAGTCCGAAG CCGAAGCTGA CCAGCAGATG 
GACAACTTGT ACCTGAAAGC CTTGGAGGGT TTCATTGCCG TGGTGACCCA AGATGGCGAC 
ATGATCTTTC TGTCAGAAAA CATCAGCAAG TTCATGGGAC TTACACAGGT GGAGCTAACA 
GGACATAGTA TCTTTGACTT CACTCATCCC TGCGACCATG AGGAGATTCG TGAGAACCTG 
AGTCTCAAAA ATGGCTCTGG TTTTGGGAAA AAAAGCAAAG ACATGTCCAC AGAGCGGGAC 
TTCTTCATGA GGATGAAGTG CACGGTCACC AACAGAGGCC GTACTGTCAA CCTCAAGTCA 
GCCACCTGGA AGGTCTTGCA CTGCACGGGC CAGGTGAAAG TCTACAACAA CTGCCCTCCT 
CACAATAGTC TGTGTGGCTA CAAGGAGCCC CTGCTGTCCT GCCTCATCAT CATGTGTGAA 
CCAATCCAGC ACCCATCCCA CATGGACATC CCCCTGGATA GCAAGACCTT CCTGAGCCGC 
CACAGCATGG ACATGAAGTT CACCTACTGT GATGACAGAA TCACAGAACT GATTGGTTAC 
CACCCTGAGG AGCTGCTTGG CCGCTCAGCC TATGAATTCT ACCATGCGCT AGACTCCGAG 
AACATGACCA AGAGTCACCA GAACTTGTGC ACCAAGGGTC AGGTAGTAAG TGGCCAGTAC 
CGGATGCTCG CAAAGCATGG GGGCTACGTG TGGCTGGAGA CCCAGGGGAC GGTCATCTAC 
AACCCTCGCA ACCTGCAGCC CCAGTGCATC ATGTGTGTCA ACTACGTCCT GAGTGAGATT 
GAGAAGAATG ACGTGGTGTT CTCCATGGAC CAGACTGAAT CCCTGTTCAA GCCCCACCTG 
ATGGCCATGA ACAGCATCTT TGATAGCAGT GGCAAGGGGG CTGTGTCTGA GAAGAGTAAC 
TTCCTATTCA CCAAGCTAAA GGAGGAGCCC GAGGAGCTGG CCCAGCTGGC TCCCACCCCA 
GGAGACGCCA TCATCTCTCT GGATTTCGGG AATCAGAACT TCGAGGAGTC CTCAGCCTAT 
GGCAAGGCCA TCCTGCCCCC GAGCCAGCCA TGGGCCACGG AGTTGAGGAG CCACAGCACC 
CAGAGCGAGG CTGGGAGCCT GCCTGCCTTC ACCGTGCCCC AGGCAGCTGC CCCGGGCAGC 
ACCACCCCCA GTGCCACCAG CAGCAGCAGC AGCTGCTCCA CGCCCAATAG CCCTGAAGAC 
TATTACACAT CTTTGGATAA CGACCTGAAG ATTGAAGTGA TTGAGAAGCT CTTCGCCATG 
GACACAGAGG CCAAGGACCA ATGCAGTACC CAGACGGATT TCAATGAGCT GGACTTGGAG 
ACACTGGCAC CCTATATCCC CATGGACGGG GAAGACTTCC AGCTAAGCCC CATCTGCCCC 
GAGGAGCGGC TCTTGGCGGA GAACCCACAG TCCACCCCCC AGCACTGCTT CAGTGCCATG 
ACAAACATCT TCCAGCCACT GGCCCCTGTA GCCCCGCACA GTCCCTTCCT CCTGGACAAG 
TTTCAGCAGC AGCTGGAGAG CAAGAAGACA GAGCCCGAGC ACCGGCCCAT GTCCTCCATC 
TTCTTTGATG CCGGAAGCAA AGCATCCCTG CCACCGTGCT GTGGCCAGGC CAGCACCCCT 
CTCTCTTCCA TGGGGGGCAG ATCCAATACC CAGTGGCCCC CAGATCCACC ATTACATTTT 
GGGCCCACAA AGTGGGCCGT CGGGGATCAG CGCACAGAGT TCTTGGGAGC AGCGCCGTTG 
GGGCCCCCTG TCTCTCCACC CCATGTCTCC ACCTTCAAGA CAAGGTCTGC AAAGGGTTTT 
GGGGCTCGAG GCCCAGACGT GCTGAGTCCG GCCATGGTAG CCCTCTCCAA CAAGCTGAAG 
CTGAAGCGAC AGCTGGAGTA TGAAGAGCAA GCCTTCCAGG ACCTGAGCGG GGGGGACCCA 
CCTGGTGGCA GCACCTCACA TTTGATGTGG AAACGGATGA AGAACCTCAG GGGTGGGAGC 
TGCCCTTTGA TGCCGGACAA GCCACTGAGC GCAAATGTAC CCAATGATAA GTTCACCCAA 
AACCCCATGA GGGGCCTGGG CCATCCCCTG AGACATCTGC CGCTGCCACA GCCTCCATCT 
GCCATCAGTC CCGGGGAGAA CAGCAAGAGC AGGTTCCCCC CACAGTGCTA CGCCACCCAG 
TACCAGGACT ACAGCCTGTC GTCAGCCCAC AAGGTGTCAG GCATGGCAAG CCGGCTGCTC 
GGGCCCTCAT TTGAGTCCTA CCTGCTGCCC GAACTGACCA GATATGACTG TGAGGTGAAC 
GTGCCCGTGC TGGGAAGCTC CACGCTCCTG CAAGGAGGGG ACCTCCTCAG AGCCCTGGAC 
CAGGCCACCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011531000.1
CDS230..2881
Misc Feature(1)302..472
Misc Feature(2)order(311..316,332..340,344..346,416..418,425..427)
Misc Feature(3)335..337
Misc Feature(4)order(353..358,365..370,374..379,428..430,437..439,449..451,455..460,470..472)
Misc Feature(5)524..709
Misc Feature(6)989..1288
Misc Feature(7)1028..1291
Misc Feature(8)order(1037..1039,1049..1051,1067..1069,1106..1117,1193..1195,1208..1210)
Misc Feature(9)order(1097..1099,1109..1111,1133..1135,1142..1147,1229..1231,1235..1237)
Misc Feature(10)1817..1915
Misc Feature(11)2765..2875
Translation

Target ORF information:

RefSeq Version XM_011532698.2
Organism Homo sapiens(human)
Definition Homo sapiens endothelial PAS domain protein 1 (EPAS1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011532698.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGTTAGGGC TTTTTGTTTT TAAAACAGGG ACACAAAGAA GAAACAGAAG GCTGCCATAT 
GCAAGGAGTA GCTCGGAGAG GAGGAAGGAG AAGTCCCGGG ATGCTGCGCG GTGCCGGCGG 
AGCAAGGAGA CGGAGGTGTT CTATGAGCTG GCCCATGAGC TGCCTCTGCC CCACAGTGTG 
AGCTCCCATC TGGACAAGGC CTCCATCATG CGACTGGCAA TCAGCTTCCT GCGAACACAC 
AAGCTCCTCT CCTCAGTTTG CTCTGAAAAC GAGTCCGAAG CCGAAGCTGA CCAGCAGATG 
GACAACTTGT ACCTGAAAGC CTTGGAGGGT TTCATTGCCG TGGTGACCCA AGATGGCGAC 
ATGATCTTTC TGTCAGAAAA CATCAGCAAG TTCATGGGAC TTACACAGGT GGAGCTAACA 
GGACATAGTA TCTTTGACTT CACTCATCCC TGCGACCATG AGGAGATTCG TGAGAACCTG 
AGTCTCAAAA ATGGCTCTGG TTTTGGGAAA AAAAGCAAAG ACATGTCCAC AGAGCGGGAC 
TTCTTCATGA GGATGAAGTG CACGGTCACC AACAGAGGCC GTACTGTCAA CCTCAAGTCA 
GCCACCTGGA AGGTCTTGCA CTGCACGGGC CAGGTGAAAG TCTACAACAA CTGCCCTCCT 
CACAATAGTC TGTGTGGCTA CAAGGAGCCC CTGCTGTCCT GCCTCATCAT CATGTGTGAA 
CCAATCCAGC ACCCATCCCA CATGGACATC CCCCTGGATA GCAAGACCTT CCTGAGCCGC 
CACAGCATGG ACATGAAGTT CACCTACTGT GATGACAGAA TCACAGAACT GATTGGTTAC 
CACCCTGAGG AGCTGCTTGG CCGCTCAGCC TATGAATTCT ACCATGCGCT AGACTCCGAG 
AACATGACCA AGAGTCACCA GAACTTGTGC ACCAAGGGTC AGGTAGTAAG TGGCCAGTAC 
CGGATGCTCG CAAAGCATGG GGGCTACGTG TGGCTGGAGA CCCAGGGGAC GGTCATCTAC 
AACCCTCGCA ACCTGCAGCC CCAGTGCATC ATGTGTGTCA ACTACGTCCT GAGTGAGATT 
GAGAAGAATG ACGTGGTGTT CTCCATGGAC CAGACTGAAT CCCTGTTCAA GCCCCACCTG 
ATGGCCATGA ACAGCATCTT TGATAGCAGT GGCAAGGGGG CTGTGTCTGA GAAGAGTAAC 
TTCCTATTCA CCAAGCTAAA GGAGGAGCCC GAGGAGCTGG CCCAGCTGGC TCCCACCCCA 
GGAGACGCCA TCATCTCTCT GGATTTCGGG AATCAGAACT TCGAGGAGTC CTCAGCCTAT 
GGCAAGGCCA TCCTGCCCCC GAGCCAGCCA TGGGCCACGG AGTTGAGGAG CCACAGCACC 
CAGAGCGAGG CTGGGAGCCT GCCTGCCTTC ACCGTGCCCC AGGCAGCTGC CCCGGGCAGC 
ACCACCCCCA GTGCCACCAG CAGCAGCAGC AGCTGCTCCA CGCCCAATAG CCCTGAAGAC 
TATTACACAT CTTTGGATAA CGACCTGAAG ATTGAAGTGA TTGAGAAGCT CTTCGCCATG 
GACACAGAGG CCAAGGACCA ATGCAGTACC CAGACGGATT TCAATGAGCT GGACTTGGAG 
ACACTGGCAC CCTATATCCC CATGGACGGG GAAGACTTCC AGCTAAGCCC CATCTGCCCC 
GAGGAGCGGC TCTTGGCGGA GAACCCACAG TCCACCCCCC AGCACTGCTT CAGTGCCATG 
ACAAACATCT TCCAGCCACT GGCCCCTGTA GCCCCGCACA GTCCCTTCCT CCTGGACAAG 
TTTCAGCAGC AGCTGGAGAG CAAGAAGACA GAGCCCGAGC ACCGGCCCAT GTCCTCCATC 
TTCTTTGATG CCGGAAGCAA AGCATCCCTG CCACCGTGCT GTGGCCAGGC CAGCACCCCT 
CTCTCTTCCA TGGGGGGCAG ATCCAATACC CAGTGGCCCC CAGATCCACC ATTACATTTT 
GGGCCCACAA AGTGGGCCGT CGGGGATCAG CGCACAGAGT TCTTGGGAGC AGCGCCGTTG 
GGGCCCCCTG TCTCTCCACC CCATGTCTCC ACCTTCAAGA CAAGGTCTGC AAAGGGTTTT 
GGGGCTCGAG GCCCAGACGT GCTGAGTCCG GCCATGGTAG CCCTCTCCAA CAAGCTGAAG 
CTGAAGCGAC AGCTGGAGTA TGAAGAGCAA GCCTTCCAGG ACCTGAGCGG GGGGGACCCA 
CCTGGTGGCA GCACCTCACA TTTGATGTGG AAACGGATGA AGAACCTCAG GGGTGGGAGC 
TGCCCTTTGA TGCCGGACAA GCCACTGAGC GCAAATGTAC CCAATGATAA GTTCACCCAA 
AACCCCATGA GGGGCCTGGG CCATCCCCTG AGACATCTGC CGCTGCCACA GCCTCCATCT 
GCCATCAGTC CCGGGGAGAA CAGCAAGAGC AGGTTCCCCC CACAGTGCTA CGCCACCCAG 
TACCAGGACT ACAGCCTGTC GTCAGCCCAC AAGGTGTCAG GCATGGCAAG CCGGCTGCTC 
GGGCCCTCAT TTGAGTCCTA CCTGCTGCCC GAACTGACCA GATATGACTG TGAGGTGAAC 
GTGCCCGTGC TGGGAAGCTC CACGCTCCTG CAAGGAGGGG ACCTCCTCAG AGCCCTGGAC 
CAGGCCACCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Activation of intestinal hypoxia-inducible factor 2? during obesity contributes to hepatic steatosis.
Nature medicine23(11)1298-1308(2017 Nov)
Xie C,Yagai T,Luo Y,Liang X,Chen T,Wang Q,Sun D,Zhao J,Ramakrishnan SK,Sun L,Jiang C,Xue X,Tian Y,Krausz KW,Patterson AD,Shah YM,Wu Y,Jiang C,Gonzalez FJ


book

PHGDH as a Key Enzyme for Serine Biosynthesis in HIF2?-Targeting Therapy for Renal Cell Carcinoma.
Cancer research77(22)6321-6329(2017 Nov)
Yoshino H,Nohata N,Miyamoto K,Yonemori M,Sakaguchi T,Sugita S,Itesako T,Kofuji S,Nakagawa M,Dahiya R,Enokida H


book

Hypoxia-inducible factor 2? (HIF-2?) promotes colon cancer growth by potentiating Yes-associated protein 1 (YAP1) activity.
The Journal of biological chemistry292(41)17046-17056(2017 10)
Ma X,Zhang H,Xue X,Shah YM


book

Preferential activation of HIF-2? adaptive signalling in neuronal-like cells in response to acute hypoxia.
PloS one12(10)e0185664(2017)
Mart?n-Arag?n Baudel MAS,Rae MT,Darlison MG,Poole AV,Fraser JA


book

Restoration of the prolyl-hydroxylase domain protein-3 oxygen-sensing mechanism is responsible for regulation of HIF2? expression and induction of sensitivity of myeloma cells to hypoxia-mediated apoptosis.
PloS one12(12)e0188438(2017)
Gastelum G,Poteshkina A,Veena M,Artiga E,Weckstein G,Frost P