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EPAS1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol EPAS1
Entrez Gene ID 2034
Full Name endothelial PAS domain protein 1
Synonyms ECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73
General protein information
Preferred Names
endothelial PAS domain-containing protein 1
Names
endothelial PAS domain-containing protein 1
EPAS-1
HIF2-alpha
HIF-2-alpha
HIF-1alpha-like factor
HIF-1-alpha-like factor
member of PAS protein 2
PAS domain-containing protein 2
hypoxia-inducible factor 2 alpha
hypoxia-inducible factor 2-alpha
basic-helix-loop-helix-PAS protein MOP2
class E basic helix-loop-helix protein 73
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2p21-p16

Summary This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]. lac of sum
Disorder MIM:

603349

Disorder Html: Erythrocytosis, familial, 4, 611783 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_011532698 XP_011531000 endothelial PAS domain-containing protein 1 isoform X1
NM_001430 NP_001421 endothelial PAS domain-containing protein 1



Homo sapiens (human) EPAS1 NP_001421.2
Pan troglodytes (chimpanzee) EPAS1 XP_001147219.1
Macaca mulatta (Rhesus monkey) EPAS1 XP_001112947.2
Canis lupus familiaris (dog) EPAS1 XP_005626137.1
Bos taurus (cattle) EPAS1 NP_777150.1
Mus musculus (house mouse) Epas1 NP_034267.3
Rattus norvegicus (Norway rat) Epas1 NP_075578.1
Gallus gallus (chicken) EPAS1 NP_990138.1
Danio rerio (zebrafish) epas1b NP_001034895.1
Xenopus (Silurana) tropicalis (western clawed frog) epas1 NP_001005647.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EPAS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EPAS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu57508 XM_011532698 PREDICTED: Homo sapiens endothelial PAS domain protein 1 (EPAS1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu23001
NM_001430 Homo sapiens endothelial PAS domain protein 1 (EPAS1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu57508
Clone ID Related Accession (Same CDS sequence) XM_011532698
Accession Version XM_011532698.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2652bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product endothelial PAS domain-containing protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022184.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGTTAGGGC TTTTTGTTTT TAAAACAGGG ACACAAAGAA GAAACAGAAG GCTGCCATAT 
GCAAGGAGTA GCTCGGAGAG GAGGAAGGAG AAGTCCCGGG ATGCTGCGCG GTGCCGGCGG
AGCAAGGAGA CGGAGGTGTT CTATGAGCTG GCCCATGAGC TGCCTCTGCC CCACAGTGTG
AGCTCCCATC TGGACAAGGC CTCCATCATG CGACTGGCAA TCAGCTTCCT GCGAACACAC
AAGCTCCTCT CCTCAGTTTG CTCTGAAAAC GAGTCCGAAG CCGAAGCTGA CCAGCAGATG
GACAACTTGT ACCTGAAAGC CTTGGAGGGT TTCATTGCCG TGGTGACCCA AGATGGCGAC
ATGATCTTTC TGTCAGAAAA CATCAGCAAG TTCATGGGAC TTACACAGGT GGAGCTAACA
GGACATAGTA TCTTTGACTT CACTCATCCC TGCGACCATG AGGAGATTCG TGAGAACCTG
AGTCTCAAAA ATGGCTCTGG TTTTGGGAAA AAAAGCAAAG ACATGTCCAC AGAGCGGGAC
TTCTTCATGA GGATGAAGTG CACGGTCACC AACAGAGGCC GTACTGTCAA CCTCAAGTCA
GCCACCTGGA AGGTCTTGCA CTGCACGGGC CAGGTGAAAG TCTACAACAA CTGCCCTCCT
CACAATAGTC TGTGTGGCTA CAAGGAGCCC CTGCTGTCCT GCCTCATCAT CATGTGTGAA
CCAATCCAGC ACCCATCCCA CATGGACATC CCCCTGGATA GCAAGACCTT CCTGAGCCGC
CACAGCATGG ACATGAAGTT CACCTACTGT GATGACAGAA TCACAGAACT GATTGGTTAC
CACCCTGAGG AGCTGCTTGG CCGCTCAGCC TATGAATTCT ACCATGCGCT AGACTCCGAG
AACATGACCA AGAGTCACCA GAACTTGTGC ACCAAGGGTC AGGTAGTAAG TGGCCAGTAC
CGGATGCTCG CAAAGCATGG GGGCTACGTG TGGCTGGAGA CCCAGGGGAC GGTCATCTAC
AACCCTCGCA ACCTGCAGCC CCAGTGCATC ATGTGTGTCA ACTACGTCCT GAGTGAGATT
GAGAAGAATG ACGTGGTGTT CTCCATGGAC CAGACTGAAT CCCTGTTCAA GCCCCACCTG
ATGGCCATGA ACAGCATCTT TGATAGCAGT GGCAAGGGGG CTGTGTCTGA GAAGAGTAAC
TTCCTATTCA CCAAGCTAAA GGAGGAGCCC GAGGAGCTGG CCCAGCTGGC TCCCACCCCA
GGAGACGCCA TCATCTCTCT GGATTTCGGG AATCAGAACT TCGAGGAGTC CTCAGCCTAT
GGCAAGGCCA TCCTGCCCCC GAGCCAGCCA TGGGCCACGG AGTTGAGGAG CCACAGCACC
CAGAGCGAGG CTGGGAGCCT GCCTGCCTTC ACCGTGCCCC AGGCAGCTGC CCCGGGCAGC
ACCACCCCCA GTGCCACCAG CAGCAGCAGC AGCTGCTCCA CGCCCAATAG CCCTGAAGAC
TATTACACAT CTTTGGATAA CGACCTGAAG ATTGAAGTGA TTGAGAAGCT CTTCGCCATG
GACACAGAGG CCAAGGACCA ATGCAGTACC CAGACGGATT TCAATGAGCT GGACTTGGAG
ACACTGGCAC CCTATATCCC CATGGACGGG GAAGACTTCC AGCTAAGCCC CATCTGCCCC
GAGGAGCGGC TCTTGGCGGA GAACCCACAG TCCACCCCCC AGCACTGCTT CAGTGCCATG
ACAAACATCT TCCAGCCACT GGCCCCTGTA GCCCCGCACA GTCCCTTCCT CCTGGACAAG
TTTCAGCAGC AGCTGGAGAG CAAGAAGACA GAGCCCGAGC ACCGGCCCAT GTCCTCCATC
TTCTTTGATG CCGGAAGCAA AGCATCCCTG CCACCGTGCT GTGGCCAGGC CAGCACCCCT
CTCTCTTCCA TGGGGGGCAG ATCCAATACC CAGTGGCCCC CAGATCCACC ATTACATTTT
GGGCCCACAA AGTGGGCCGT CGGGGATCAG CGCACAGAGT TCTTGGGAGC AGCGCCGTTG
GGGCCCCCTG TCTCTCCACC CCATGTCTCC ACCTTCAAGA CAAGGTCTGC AAAGGGTTTT
GGGGCTCGAG GCCCAGACGT GCTGAGTCCG GCCATGGTAG CCCTCTCCAA CAAGCTGAAG
CTGAAGCGAC AGCTGGAGTA TGAAGAGCAA GCCTTCCAGG ACCTGAGCGG GGGGGACCCA
CCTGGTGGCA GCACCTCACA TTTGATGTGG AAACGGATGA AGAACCTCAG GGGTGGGAGC
TGCCCTTTGA TGCCGGACAA GCCACTGAGC GCAAATGTAC CCAATGATAA GTTCACCCAA
AACCCCATGA GGGGCCTGGG CCATCCCCTG AGACATCTGC CGCTGCCACA GCCTCCATCT
GCCATCAGTC CCGGGGAGAA CAGCAAGAGC AGGTTCCCCC CACAGTGCTA CGCCACCCAG
TACCAGGACT ACAGCCTGTC GTCAGCCCAC AAGGTGTCAG GCATGGCAAG CCGGCTGCTC
GGGCCCTCAT TTGAGTCCTA CCTGCTGCCC GAACTGACCA GATATGACTG TGAGGTGAAC
GTGCCCGTGC TGGGAAGCTC CACGCTCCTG CAAGGAGGGG ACCTCCTCAG AGCCCTGGAC
CAGGCCACCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011531000.1
CDS16..2667
Misc Feature(1)88..258(+)
Misc Feature(2)97..213(+)
Misc Feature(3)121..123(+)
Misc Feature(4)139..258(+)
Misc Feature(5)343..>495(+)
Misc Feature(6)385..465(+)
Misc Feature(7)445..495(+)
Misc Feature(8)775..1074(+)
Misc Feature(9)784..1092(+)
Misc Feature(10)823..996(+)
Misc Feature(11)883..1023(+)
Misc Feature(12)1600..1704(+)
Misc Feature(13)2545..2664(+)
Translation

Target ORF information:

RefSeq Version XM_011532698
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens endothelial PAS domain protein 1 (EPAS1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011532698

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGTTAGGGC TTTTTGTTTT TAAAACAGGG ACACAAAGAA GAAACAGAAG GCTGCCATAT 
GCAAGGAGTA GCTCGGAGAG GAGGAAGGAG AAGTCCCGGG ATGCTGCGCG GTGCCGGCGG
AGCAAGGAGA CGGAGGTGTT CTATGAGCTG GCCCATGAGC TGCCTCTGCC CCACAGTGTG
AGCTCCCATC TGGACAAGGC CTCCATCATG CGACTGGCAA TCAGCTTCCT GCGAACACAC
AAGCTCCTCT CCTCAGTTTG CTCTGAAAAC GAGTCCGAAG CCGAAGCTGA CCAGCAGATG
GACAACTTGT ACCTGAAAGC CTTGGAGGGT TTCATTGCCG TGGTGACCCA AGATGGCGAC
ATGATCTTTC TGTCAGAAAA CATCAGCAAG TTCATGGGAC TTACACAGGT GGAGCTAACA
GGACATAGTA TCTTTGACTT CACTCATCCC TGCGACCATG AGGAGATTCG TGAGAACCTG
AGTCTCAAAA ATGGCTCTGG TTTTGGGAAA AAAAGCAAAG ACATGTCCAC AGAGCGGGAC
TTCTTCATGA GGATGAAGTG CACGGTCACC AACAGAGGCC GTACTGTCAA CCTCAAGTCA
GCCACCTGGA AGGTCTTGCA CTGCACGGGC CAGGTGAAAG TCTACAACAA CTGCCCTCCT
CACAATAGTC TGTGTGGCTA CAAGGAGCCC CTGCTGTCCT GCCTCATCAT CATGTGTGAA
CCAATCCAGC ACCCATCCCA CATGGACATC CCCCTGGATA GCAAGACCTT CCTGAGCCGC
CACAGCATGG ACATGAAGTT CACCTACTGT GATGACAGAA TCACAGAACT GATTGGTTAC
CACCCTGAGG AGCTGCTTGG CCGCTCAGCC TATGAATTCT ACCATGCGCT AGACTCCGAG
AACATGACCA AGAGTCACCA GAACTTGTGC ACCAAGGGTC AGGTAGTAAG TGGCCAGTAC
CGGATGCTCG CAAAGCATGG GGGCTACGTG TGGCTGGAGA CCCAGGGGAC GGTCATCTAC
AACCCTCGCA ACCTGCAGCC CCAGTGCATC ATGTGTGTCA ACTACGTCCT GAGTGAGATT
GAGAAGAATG ACGTGGTGTT CTCCATGGAC CAGACTGAAT CCCTGTTCAA GCCCCACCTG
ATGGCCATGA ACAGCATCTT TGATAGCAGT GGCAAGGGGG CTGTGTCTGA GAAGAGTAAC
TTCCTATTCA CCAAGCTAAA GGAGGAGCCC GAGGAGCTGG CCCAGCTGGC TCCCACCCCA
GGAGACGCCA TCATCTCTCT GGATTTCGGG AATCAGAACT TCGAGGAGTC CTCAGCCTAT
GGCAAGGCCA TCCTGCCCCC GAGCCAGCCA TGGGCCACGG AGTTGAGGAG CCACAGCACC
CAGAGCGAGG CTGGGAGCCT GCCTGCCTTC ACCGTGCCCC AGGCAGCTGC CCCGGGCAGC
ACCACCCCCA GTGCCACCAG CAGCAGCAGC AGCTGCTCCA CGCCCAATAG CCCTGAAGAC
TATTACACAT CTTTGGATAA CGACCTGAAG ATTGAAGTGA TTGAGAAGCT CTTCGCCATG
GACACAGAGG CCAAGGACCA ATGCAGTACC CAGACGGATT TCAATGAGCT GGACTTGGAG
ACACTGGCAC CCTATATCCC CATGGACGGG GAAGACTTCC AGCTAAGCCC CATCTGCCCC
GAGGAGCGGC TCTTGGCGGA GAACCCACAG TCCACCCCCC AGCACTGCTT CAGTGCCATG
ACAAACATCT TCCAGCCACT GGCCCCTGTA GCCCCGCACA GTCCCTTCCT CCTGGACAAG
TTTCAGCAGC AGCTGGAGAG CAAGAAGACA GAGCCCGAGC ACCGGCCCAT GTCCTCCATC
TTCTTTGATG CCGGAAGCAA AGCATCCCTG CCACCGTGCT GTGGCCAGGC CAGCACCCCT
CTCTCTTCCA TGGGGGGCAG ATCCAATACC CAGTGGCCCC CAGATCCACC ATTACATTTT
GGGCCCACAA AGTGGGCCGT CGGGGATCAG CGCACAGAGT TCTTGGGAGC AGCGCCGTTG
GGGCCCCCTG TCTCTCCACC CCATGTCTCC ACCTTCAAGA CAAGGTCTGC AAAGGGTTTT
GGGGCTCGAG GCCCAGACGT GCTGAGTCCG GCCATGGTAG CCCTCTCCAA CAAGCTGAAG
CTGAAGCGAC AGCTGGAGTA TGAAGAGCAA GCCTTCCAGG ACCTGAGCGG GGGGGACCCA
CCTGGTGGCA GCACCTCACA TTTGATGTGG AAACGGATGA AGAACCTCAG GGGTGGGAGC
TGCCCTTTGA TGCCGGACAA GCCACTGAGC GCAAATGTAC CCAATGATAA GTTCACCCAA
AACCCCATGA GGGGCCTGGG CCATCCCCTG AGACATCTGC CGCTGCCACA GCCTCCATCT
GCCATCAGTC CCGGGGAGAA CAGCAAGAGC AGGTTCCCCC CACAGTGCTA CGCCACCCAG
TACCAGGACT ACAGCCTGTC GTCAGCCCAC AAGGTGTCAG GCATGGCAAG CCGGCTGCTC
GGGCCCTCAT TTGAGTCCTA CCTGCTGCCC GAACTGACCA GATATGACTG TGAGGTGAAC
GTGCCCGTGC TGGGAAGCTC CACGCTCCTG CAAGGAGGGG ACCTCCTCAG AGCCCTGGAC
CAGGCCACCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23001
Clone ID Related Accession (Same CDS sequence) NM_001430
Accession Version NM_001430.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2613bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product endothelial PAS domain-containing protein 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC016696.8, CD109908.1, BC051338.1, AK123845.1 and BM671511.1. This sequence is a reference standard in the RefSeqGene project. On Nov 6, 2009 this sequence version replaced gi:41327154. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC051338.1, U81984.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGACAGCTG ACAAGGAGAA GAAAAGGAGT AGCTCGGAGA GGAGGAAGGA GAAGTCCCGG 
GATGCTGCGC GGTGCCGGCG GAGCAAGGAG ACGGAGGTGT TCTATGAGCT GGCCCATGAG
CTGCCTCTGC CCCACAGTGT GAGCTCCCAT CTGGACAAGG CCTCCATCAT GCGACTGGCA
ATCAGCTTCC TGCGAACACA CAAGCTCCTC TCCTCAGTTT GCTCTGAAAA CGAGTCCGAA
GCCGAAGCTG ACCAGCAGAT GGACAACTTG TACCTGAAAG CCTTGGAGGG TTTCATTGCC
GTGGTGACCC AAGATGGCGA CATGATCTTT CTGTCAGAAA ACATCAGCAA GTTCATGGGA
CTTACACAGG TGGAGCTAAC AGGACATAGT ATCTTTGACT TCACTCATCC CTGCGACCAT
GAGGAGATTC GTGAGAACCT GAGTCTCAAA AATGGCTCTG GTTTTGGGAA AAAAAGCAAA
GACATGTCCA CAGAGCGGGA CTTCTTCATG AGGATGAAGT GCACGGTCAC CAACAGAGGC
CGTACTGTCA ACCTCAAGTC AGCCACCTGG AAGGTCTTGC ACTGCACGGG CCAGGTGAAA
GTCTACAACA ACTGCCCTCC TCACAATAGT CTGTGTGGCT ACAAGGAGCC CCTGCTGTCC
TGCCTCATCA TCATGTGTGA ACCAATCCAG CACCCATCCC ACATGGACAT CCCCCTGGAT
AGCAAGACCT TCCTGAGCCG CCACAGCATG GACATGAAGT TCACCTACTG TGATGACAGA
ATCACAGAAC TGATTGGTTA CCACCCTGAG GAGCTGCTTG GCCGCTCAGC CTATGAATTC
TACCATGCGC TAGACTCCGA GAACATGACC AAGAGTCACC AGAACTTGTG CACCAAGGGT
CAGGTAGTAA GTGGCCAGTA CCGGATGCTC GCAAAGCATG GGGGCTACGT GTGGCTGGAG
ACCCAGGGGA CGGTCATCTA CAACCCTCGC AACCTGCAGC CCCAGTGCAT CATGTGTGTC
AACTACGTCC TGAGTGAGAT TGAGAAGAAT GACGTGGTGT TCTCCATGGA CCAGACTGAA
TCCCTGTTCA AGCCCCACCT GATGGCCATG AACAGCATCT TTGATAGCAG TGGCAAGGGG
GCTGTGTCTG AGAAGAGTAA CTTCCTATTC ACCAAGCTAA AGGAGGAGCC CGAGGAGCTG
GCCCAGCTGG CTCCCACCCC AGGAGACGCC ATCATCTCTC TGGATTTCGG GAATCAGAAC
TTCGAGGAGT CCTCAGCCTA TGGCAAGGCC ATCCTGCCCC CGAGCCAGCC ATGGGCCACG
GAGTTGAGGA GCCACAGCAC CCAGAGCGAG GCTGGGAGCC TGCCTGCCTT CACCGTGCCC
CAGGCAGCTG CCCCGGGCAG CACCACCCCC AGTGCCACCA GCAGCAGCAG CAGCTGCTCC
ACGCCCAATA GCCCTGAAGA CTATTACACA TCTTTGGATA ACGACCTGAA GATTGAAGTG
ATTGAGAAGC TCTTCGCCAT GGACACAGAG GCCAAGGACC AATGCAGTAC CCAGACGGAT
TTCAATGAGC TGGACTTGGA GACACTGGCA CCCTATATCC CCATGGACGG GGAAGACTTC
CAGCTAAGCC CCATCTGCCC CGAGGAGCGG CTCTTGGCGG AGAACCCACA GTCCACCCCC
CAGCACTGCT TCAGTGCCAT GACAAACATC TTCCAGCCAC TGGCCCCTGT AGCCCCGCAC
AGTCCCTTCC TCCTGGACAA GTTTCAGCAG CAGCTGGAGA GCAAGAAGAC AGAGCCCGAG
CACCGGCCCA TGTCCTCCAT CTTCTTTGAT GCCGGAAGCA AAGCATCCCT GCCACCGTGC
TGTGGCCAGG CCAGCACCCC TCTCTCTTCC ATGGGGGGCA GATCCAATAC CCAGTGGCCC
CCAGATCCAC CATTACATTT TGGGCCCACA AAGTGGGCCG TCGGGGATCA GCGCACAGAG
TTCTTGGGAG CAGCGCCGTT GGGGCCCCCT GTCTCTCCAC CCCATGTCTC CACCTTCAAG
ACAAGGTCTG CAAAGGGTTT TGGGGCTCGA GGCCCAGACG TGCTGAGTCC GGCCATGGTA
GCCCTCTCCA ACAAGCTGAA GCTGAAGCGA CAGCTGGAGT ATGAAGAGCA AGCCTTCCAG
GACCTGAGCG GGGGGGACCC ACCTGGTGGC AGCACCTCAC ATTTGATGTG GAAACGGATG
AAGAACCTCA GGGGTGGGAG CTGCCCTTTG ATGCCGGACA AGCCACTGAG CGCAAATGTA
CCCAATGATA AGTTCACCCA AAACCCCATG AGGGGCCTGG GCCATCCCCT GAGACATCTG
CCGCTGCCAC AGCCTCCATC TGCCATCAGT CCCGGGGAGA ACAGCAAGAG CAGGTTCCCC
CCACAGTGCT ACGCCACCCA GTACCAGGAC TACAGCCTGT CGTCAGCCCA CAAGGTGTCA
GGCATGGCAA GCCGGCTGCT CGGGCCCTCA TTTGAGTCCT ACCTGCTGCC CGAACTGACC
AGATATGACT GTGAGGTGAA CGTGCCCGTG CTGGGAAGCT CCACGCTCCT GCAAGGAGGG
GACCTCCTCA GAGCCCTGGA CCAGGCCACC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001421.2
CDS511..3123
Misc Feature(1)484..486(+)
Misc Feature(2)544..714(+)
Misc Feature(3)553..669(+)
Misc Feature(4)577..579(+)
Misc Feature(5)595..714(+)
Misc Feature(6)799..>951(+)
Misc Feature(7)841..921(+)
Misc Feature(8)901..951(+)
Misc Feature(9)1231..1530(+)
Misc Feature(10)1240..1548(+)
Misc Feature(11)1279..1452(+)
Misc Feature(12)1339..1479(+)
Misc Feature(13)1480..1482(+)
Misc Feature(14)1996..2136(+)
Misc Feature(15)2056..2160(+)
Misc Feature(16)2998..3120(+)
Misc Feature(17)3001..3120(+)
Exon (1)1..536
Gene:EPAS1
Gene Synonym:
Exon (2)537..727
Gene:EPAS1
Gene Synonym:
Exon (3)728..879
Gene:EPAS1
Gene Synonym:
Exon (4)880..964
Gene:EPAS1
Gene Synonym:
Exon (5)965..1083
Gene:EPAS1
Gene Synonym:
Exon (6)1084..1289
Gene:EPAS1
Gene Synonym:
Exon (7)1290..1396
Gene:EPAS1
Gene Synonym:
Exon (8)1397..1544
Gene:EPAS1
Gene Synonym:
Exon (9)1545..1759
Gene:EPAS1
Gene Synonym:
Exon (10)1760..1953
Gene:EPAS1
Gene Synonym:
Exon (11)1954..2064
Gene:EPAS1
Gene Synonym:
Exon (12)2065..2555
Gene:EPAS1
Gene Synonym:
Exon (13)2556..2682
Gene:EPAS1
Gene Synonym:
Exon (14)2683..2797
Gene:EPAS1
Gene Synonym:
Exon (15)2798..2971
Gene:EPAS1
Gene Synonym:
Exon (16)2972..5166
Gene:EPAS1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001430
Organism Homo sapiens (human)
Definition Homo sapiens endothelial PAS domain protein 1 (EPAS1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001430

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGACAGCTG ACAAGGAGAA GAAAAGGAGT AGCTCGGAGA GGAGGAAGGA GAAGTCCCGG 
GATGCTGCGC GGTGCCGGCG GAGCAAGGAG ACGGAGGTGT TCTATGAGCT GGCCCATGAG
CTGCCTCTGC CCCACAGTGT GAGCTCCCAT CTGGACAAGG CCTCCATCAT GCGACTGGCA
ATCAGCTTCC TGCGAACACA CAAGCTCCTC TCCTCAGTTT GCTCTGAAAA CGAGTCCGAA
GCCGAAGCTG ACCAGCAGAT GGACAACTTG TACCTGAAAG CCTTGGAGGG TTTCATTGCC
GTGGTGACCC AAGATGGCGA CATGATCTTT CTGTCAGAAA ACATCAGCAA GTTCATGGGA
CTTACACAGG TGGAGCTAAC AGGACATAGT ATCTTTGACT TCACTCATCC CTGCGACCAT
GAGGAGATTC GTGAGAACCT GAGTCTCAAA AATGGCTCTG GTTTTGGGAA AAAAAGCAAA
GACATGTCCA CAGAGCGGGA CTTCTTCATG AGGATGAAGT GCACGGTCAC CAACAGAGGC
CGTACTGTCA ACCTCAAGTC AGCCACCTGG AAGGTCTTGC ACTGCACGGG CCAGGTGAAA
GTCTACAACA ACTGCCCTCC TCACAATAGT CTGTGTGGCT ACAAGGAGCC CCTGCTGTCC
TGCCTCATCA TCATGTGTGA ACCAATCCAG CACCCATCCC ACATGGACAT CCCCCTGGAT
AGCAAGACCT TCCTGAGCCG CCACAGCATG GACATGAAGT TCACCTACTG TGATGACAGA
ATCACAGAAC TGATTGGTTA CCACCCTGAG GAGCTGCTTG GCCGCTCAGC CTATGAATTC
TACCATGCGC TAGACTCCGA GAACATGACC AAGAGTCACC AGAACTTGTG CACCAAGGGT
CAGGTAGTAA GTGGCCAGTA CCGGATGCTC GCAAAGCATG GGGGCTACGT GTGGCTGGAG
ACCCAGGGGA CGGTCATCTA CAACCCTCGC AACCTGCAGC CCCAGTGCAT CATGTGTGTC
AACTACGTCC TGAGTGAGAT TGAGAAGAAT GACGTGGTGT TCTCCATGGA CCAGACTGAA
TCCCTGTTCA AGCCCCACCT GATGGCCATG AACAGCATCT TTGATAGCAG TGGCAAGGGG
GCTGTGTCTG AGAAGAGTAA CTTCCTATTC ACCAAGCTAA AGGAGGAGCC CGAGGAGCTG
GCCCAGCTGG CTCCCACCCC AGGAGACGCC ATCATCTCTC TGGATTTCGG GAATCAGAAC
TTCGAGGAGT CCTCAGCCTA TGGCAAGGCC ATCCTGCCCC CGAGCCAGCC ATGGGCCACG
GAGTTGAGGA GCCACAGCAC CCAGAGCGAG GCTGGGAGCC TGCCTGCCTT CACCGTGCCC
CAGGCAGCTG CCCCGGGCAG CACCACCCCC AGTGCCACCA GCAGCAGCAG CAGCTGCTCC
ACGCCCAATA GCCCTGAAGA CTATTACACA TCTTTGGATA ACGACCTGAA GATTGAAGTG
ATTGAGAAGC TCTTCGCCAT GGACACAGAG GCCAAGGACC AATGCAGTAC CCAGACGGAT
TTCAATGAGC TGGACTTGGA GACACTGGCA CCCTATATCC CCATGGACGG GGAAGACTTC
CAGCTAAGCC CCATCTGCCC CGAGGAGCGG CTCTTGGCGG AGAACCCACA GTCCACCCCC
CAGCACTGCT TCAGTGCCAT GACAAACATC TTCCAGCCAC TGGCCCCTGT AGCCCCGCAC
AGTCCCTTCC TCCTGGACAA GTTTCAGCAG CAGCTGGAGA GCAAGAAGAC AGAGCCCGAG
CACCGGCCCA TGTCCTCCAT CTTCTTTGAT GCCGGAAGCA AAGCATCCCT GCCACCGTGC
TGTGGCCAGG CCAGCACCCC TCTCTCTTCC ATGGGGGGCA GATCCAATAC CCAGTGGCCC
CCAGATCCAC CATTACATTT TGGGCCCACA AAGTGGGCCG TCGGGGATCA GCGCACAGAG
TTCTTGGGAG CAGCGCCGTT GGGGCCCCCT GTCTCTCCAC CCCATGTCTC CACCTTCAAG
ACAAGGTCTG CAAAGGGTTT TGGGGCTCGA GGCCCAGACG TGCTGAGTCC GGCCATGGTA
GCCCTCTCCA ACAAGCTGAA GCTGAAGCGA CAGCTGGAGT ATGAAGAGCA AGCCTTCCAG
GACCTGAGCG GGGGGGACCC ACCTGGTGGC AGCACCTCAC ATTTGATGTG GAAACGGATG
AAGAACCTCA GGGGTGGGAG CTGCCCTTTG ATGCCGGACA AGCCACTGAG CGCAAATGTA
CCCAATGATA AGTTCACCCA AAACCCCATG AGGGGCCTGG GCCATCCCCT GAGACATCTG
CCGCTGCCAC AGCCTCCATC TGCCATCAGT CCCGGGGAGA ACAGCAAGAG CAGGTTCCCC
CCACAGTGCT ACGCCACCCA GTACCAGGAC TACAGCCTGT CGTCAGCCCA CAAGGTGTCA
GGCATGGCAA GCCGGCTGCT CGGGCCCTCA TTTGAGTCCT ACCTGCTGCC CGAACTGACC
AGATATGACT GTGAGGTGAA CGTGCCCGTG CTGGGAAGCT CCACGCTCCT GCAAGGAGGG
GACCTCCTCA GAGCCCTGGA CCAGGCCACC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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