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EPAS1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol EPAS1
Entrez Gene ID 2034
Full Name endothelial PAS domain protein 1
Synonyms ECYT4,HIF2A,HLF,MOP2,PASD2,bHLHe73
General protein information
Preferred Names

endothelial PAS domain protein 1

Names

endothelial PAS domain-containing protein 1
EPAS-1
HIF-1-alpha-like factor
HIF-1alpha-like factor
HIF-2-alpha
HIF2-alpha
PAS domain-containing protein 2
basic-helix-loop-helix-PAS protein MOP2
class E basic helix-loop-helix protein 73
hypoxia-inducible factor 2 alpha
member of PAS protein 2

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

2

2p21

Disorder MIM:

603349

Disorder Html: Erythrocytosis, familial, 4, 611783 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_001430.4 NP_001421.2 endothelial PAS domain-containing protein 1
XM_011532698.2 XP_011531000.1 endothelial PAS domain-containing protein 1 isoform X1



Canis lupus familiaris (dog) EPAS1 XP_005626137.1
Rattus norvegicus (Norway rat) Epas1 NP_075578.1
Mus musculus (house mouse) Epas1 NP_034267.3
Macaca mulatta (rhesus monkey) EPAS1 XP_001112947.2
Pan troglodytes (chimpanzee) EPAS1 XP_001147219.1
Gallus gallus (chicken) EPAS1 NP_990138.1
Danio rerio (zebrafish) epas1b NP_001034895.1
Xenopus tropicalis (tropical clawed frog) epas1 NP_001005647.1
Homo sapiens (human) EPAS1 NP_001421.2
Bos taurus (cattle) EPAS1 NP_777150.1


Related articles in PubMed

MicroRNA-383 is a tumor suppressor in human lung cancer by targeting endothelial PAS domain-containing protein 1.
Ma H, Liu B, Wang S, Liu J
Cell biochemistry and function34(8)613-619(2016 Dec)

Correlation of HIF-1α/HIF-2α expression with FDG uptake in lung adenocarcinoma.
Higashi K, Yamagishi T, Ueda Y, Ishigaki Y, Shimasaki M, Nakamura Y, Oguchi M, Takegami T, Sagawa M, Tonami H
Annals of nuclear medicine30(10)708-715(2016 Dec)

Combined over-expression of the hypoxia-inducible factor 2α gene and its long non-coding RNA predicts unfavorable prognosis of patients with osteosarcoma.
Li W, He X, Xue R, Zhang Y, Zhang X, Lu J, Zhang Z, Xue L
Pathology, research and practice212(10)861-866(2016 Oct)

MBD3 mediates epigenetic regulation on EPAS1 promoter in cancer.
Cui J, Duan B, Zhao X, Chen Y, Sun S, Deng W, Zhang Y, Du J, Chen Y, Gu L
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(10)13455-13467(2016 Oct)

HIF1A and EPAS1 mRNA and protein expression during in vitro culture of human primary term cytotrophoblasts and effect of oxygen tension on their expression.
Depoix CL, Flabat O, Debiève F, Hubinont C
Reproductive biology16(3)203-211(2016 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results indicate that HPV16 E6/E7 indirectly upregulated the expression of VEGF by inhibition of liver kinase B1 expression and upregulation of hypoxia-inducible factor 2alpha expression,thus propose a human papillomavirus-liver kinase B1-hypoxia-inducible factor 2A-vascular endothelial growth factor axis for the tumorigenesis of lung cancer
Title: HPV16 E6/E7 upregulates HIF-2α and VEGF by inhibiting LKB1 in lung cancer cells.

Univariate analysis of the overall survival demonstrates that HIF-2a expression but not HIF-1a was related to poor outcome ( p = 0.005) and it retained significance in multivariate analysis ( p = 0.046). In conclusion, HIF-2a expression was related to tumor size, lymph node metastasis, tumor stage and histology. We also found a positive prognostic value of HIF-2a protein expression.
Title: HIF-2α not HIF-1α overexpression confers poor prognosis in non-small cell lung cancer.

The advantageous Tibetan EPAS1 haplotype shared many variants with the Denisovan genome, with an ancient gene tree divergence between the Tibetan and Denisovan haplotypes of about 1 million years ago. With the exception of EPAS1, we observed no evidence of positive selection on Denisovan-like haplotypes
Title: Evolutionary history of Tibetans inferred from whole-genome sequencing.

knockdown of HIF-2-alpha in the osteosarcoma cell line reduced the proliferation of cancer cells and increased apoptosis. These effects likely occurred through the MAPKp38 signaling pathway.
Title: HIF-2α affects proliferation and apoptosis of MG-63 osteosarcoma cells through MAPK signaling.

Results suggested that the oncogenic transcription factor HIF-2alpha stabilized VHL disease suppressor B-Myb, which is also a transcription factor, by physical interaction. Some B-Myb-dependent gene expression was similarly affected by B-Myb or HIF-2alpha knockdown, suggesting that stabilization of B-Myb by HIF-2alpha may play a role in specific gene expressions.
Title: Hypoxia-inducible factor-2α stabilizes the von Hippel-Lindau (VHL) disease suppressor, Myb-related protein 2.

The following EPAS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EPAS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu23001
NM_001430.4
Latest version!
Homo sapiens endothelial PAS domain protein 1 (EPAS1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu57508 XM_011532698.2
Latest version!
Homo sapiens endothelial PAS domain protein 1 (EPAS1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu23001
Clone ID Related Accession (Same CDS sequence) NM_001430.4
Accession Version NM_001430.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2613bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-09
Organism Homo sapiens(Human)
Product endothelial PAS domain-containing protein 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC016696.8, CD109908.1, BC051338.1, AK123845.1 and BM671511.1. This sequence is a reference standard in the RefSeqGene project. On Nov 6, 2009 this sequence version replaced NM_001430.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC051338.1, U81984.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGACAGCTG ACAAGGAGAA GAAAAGGAGT AGCTCGGAGA GGAGGAAGGA GAAGTCCCGG 
GATGCTGCGC GGTGCCGGCG GAGCAAGGAG ACGGAGGTGT TCTATGAGCT GGCCCATGAG
CTGCCTCTGC CCCACAGTGT GAGCTCCCAT CTGGACAAGG CCTCCATCAT GCGACTGGCA
ATCAGCTTCC TGCGAACACA CAAGCTCCTC TCCTCAGTTT GCTCTGAAAA CGAGTCCGAA
GCCGAAGCTG ACCAGCAGAT GGACAACTTG TACCTGAAAG CCTTGGAGGG TTTCATTGCC
GTGGTGACCC AAGATGGCGA CATGATCTTT CTGTCAGAAA ACATCAGCAA GTTCATGGGA
CTTACACAGG TGGAGCTAAC AGGACATAGT ATCTTTGACT TCACTCATCC CTGCGACCAT
GAGGAGATTC GTGAGAACCT GAGTCTCAAA AATGGCTCTG GTTTTGGGAA AAAAAGCAAA
GACATGTCCA CAGAGCGGGA CTTCTTCATG AGGATGAAGT GCACGGTCAC CAACAGAGGC
CGTACTGTCA ACCTCAAGTC AGCCACCTGG AAGGTCTTGC ACTGCACGGG CCAGGTGAAA
GTCTACAACA ACTGCCCTCC TCACAATAGT CTGTGTGGCT ACAAGGAGCC CCTGCTGTCC
TGCCTCATCA TCATGTGTGA ACCAATCCAG CACCCATCCC ACATGGACAT CCCCCTGGAT
AGCAAGACCT TCCTGAGCCG CCACAGCATG GACATGAAGT TCACCTACTG TGATGACAGA
ATCACAGAAC TGATTGGTTA CCACCCTGAG GAGCTGCTTG GCCGCTCAGC CTATGAATTC
TACCATGCGC TAGACTCCGA GAACATGACC AAGAGTCACC AGAACTTGTG CACCAAGGGT
CAGGTAGTAA GTGGCCAGTA CCGGATGCTC GCAAAGCATG GGGGCTACGT GTGGCTGGAG
ACCCAGGGGA CGGTCATCTA CAACCCTCGC AACCTGCAGC CCCAGTGCAT CATGTGTGTC
AACTACGTCC TGAGTGAGAT TGAGAAGAAT GACGTGGTGT TCTCCATGGA CCAGACTGAA
TCCCTGTTCA AGCCCCACCT GATGGCCATG AACAGCATCT TTGATAGCAG TGGCAAGGGG
GCTGTGTCTG AGAAGAGTAA CTTCCTATTC ACCAAGCTAA AGGAGGAGCC CGAGGAGCTG
GCCCAGCTGG CTCCCACCCC AGGAGACGCC ATCATCTCTC TGGATTTCGG GAATCAGAAC
TTCGAGGAGT CCTCAGCCTA TGGCAAGGCC ATCCTGCCCC CGAGCCAGCC ATGGGCCACG
GAGTTGAGGA GCCACAGCAC CCAGAGCGAG GCTGGGAGCC TGCCTGCCTT CACCGTGCCC
CAGGCAGCTG CCCCGGGCAG CACCACCCCC AGTGCCACCA GCAGCAGCAG CAGCTGCTCC
ACGCCCAATA GCCCTGAAGA CTATTACACA TCTTTGGATA ACGACCTGAA GATTGAAGTG
ATTGAGAAGC TCTTCGCCAT GGACACAGAG GCCAAGGACC AATGCAGTAC CCAGACGGAT
TTCAATGAGC TGGACTTGGA GACACTGGCA CCCTATATCC CCATGGACGG GGAAGACTTC
CAGCTAAGCC CCATCTGCCC CGAGGAGCGG CTCTTGGCGG AGAACCCACA GTCCACCCCC
CAGCACTGCT TCAGTGCCAT GACAAACATC TTCCAGCCAC TGGCCCCTGT AGCCCCGCAC
AGTCCCTTCC TCCTGGACAA GTTTCAGCAG CAGCTGGAGA GCAAGAAGAC AGAGCCCGAG
CACCGGCCCA TGTCCTCCAT CTTCTTTGAT GCCGGAAGCA AAGCATCCCT GCCACCGTGC
TGTGGCCAGG CCAGCACCCC TCTCTCTTCC ATGGGGGGCA GATCCAATAC CCAGTGGCCC
CCAGATCCAC CATTACATTT TGGGCCCACA AAGTGGGCCG TCGGGGATCA GCGCACAGAG
TTCTTGGGAG CAGCGCCGTT GGGGCCCCCT GTCTCTCCAC CCCATGTCTC CACCTTCAAG
ACAAGGTCTG CAAAGGGTTT TGGGGCTCGA GGCCCAGACG TGCTGAGTCC GGCCATGGTA
GCCCTCTCCA ACAAGCTGAA GCTGAAGCGA CAGCTGGAGT ATGAAGAGCA AGCCTTCCAG
GACCTGAGCG GGGGGGACCC ACCTGGTGGC AGCACCTCAC ATTTGATGTG GAAACGGATG
AAGAACCTCA GGGGTGGGAG CTGCCCTTTG ATGCCGGACA AGCCACTGAG CGCAAATGTA
CCCAATGATA AGTTCACCCA AAACCCCATG AGGGGCCTGG GCCATCCCCT GAGACATCTG
CCGCTGCCAC AGCCTCCATC TGCCATCAGT CCCGGGGAGA ACAGCAAGAG CAGGTTCCCC
CCACAGTGCT ACGCCACCCA GTACCAGGAC TACAGCCTGT CGTCAGCCCA CAAGGTGTCA
GGCATGGCAA GCCGGCTGCT CGGGCCCTCA TTTGAGTCCT ACCTGCTGCC CGAACTGACC
AGATATGACT GTGAGGTGAA CGTGCCCGTG CTGGGAAGCT CCACGCTCCT GCAAGGAGGG
GACCTCCTCA GAGCCCTGGA CCAGGCCACC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001421.2
CDS511..3123
Misc Feature(1)484..486
Misc Feature(2)544..714
Misc Feature(3)order(553..558,574..582,586..588,658..660,667..669)
Misc Feature(4)577..579
Misc Feature(5)order(595..600,607..612,616..621,670..672,679..681,691..693,697..702,712..714)
Misc Feature(6)766..951
Misc Feature(7)1231..1530
Misc Feature(8)1270..1533
Misc Feature(9)order(1279..1281,1291..1293,1309..1311,1348..1359,1435..1437,1450..1452)
Misc Feature(10)order(1339..1341,1351..1353,1375..1377,1384..1389,1471..1473,1477..1479)
Misc Feature(11)1480..1482
Misc Feature(12)1723..1725
Misc Feature(13)1996..2136
Misc Feature(14)2059..2157
Misc Feature(15)2101..2103
Misc Feature(16)2998..3120
Misc Feature(17)3007..3117
Misc Feature(18)3028..3030
Misc Feature(19)3049..3051
Exon (1)1..536
Exon (2)537..727
Exon (3)728..879
Exon (4)880..964
Exon (5)965..1083
Exon (6)1084..1289
Exon (7)1290..1396
Exon (8)1397..1544
Exon (9)1545..1759
Exon (10)1760..1953
Exon (11)1954..2064
Exon (12)2065..2555
Exon (13)2556..2682
Exon (14)2683..2797
Exon (15)2798..2971
Exon (16)2972..5166
Translation

Target ORF information:

RefSeq Version NM_001430.4
Organism Homo sapiens(Human)
Definition Homo sapiens endothelial PAS domain protein 1 (EPAS1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001430.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGACAGCTG ACAAGGAGAA GAAAAGGAGT AGCTCGGAGA GGAGGAAGGA GAAGTCCCGG 
GATGCTGCGC GGTGCCGGCG GAGCAAGGAG ACGGAGGTGT TCTATGAGCT GGCCCATGAG
CTGCCTCTGC CCCACAGTGT GAGCTCCCAT CTGGACAAGG CCTCCATCAT GCGACTGGCA
ATCAGCTTCC TGCGAACACA CAAGCTCCTC TCCTCAGTTT GCTCTGAAAA CGAGTCCGAA
GCCGAAGCTG ACCAGCAGAT GGACAACTTG TACCTGAAAG CCTTGGAGGG TTTCATTGCC
GTGGTGACCC AAGATGGCGA CATGATCTTT CTGTCAGAAA ACATCAGCAA GTTCATGGGA
CTTACACAGG TGGAGCTAAC AGGACATAGT ATCTTTGACT TCACTCATCC CTGCGACCAT
GAGGAGATTC GTGAGAACCT GAGTCTCAAA AATGGCTCTG GTTTTGGGAA AAAAAGCAAA
GACATGTCCA CAGAGCGGGA CTTCTTCATG AGGATGAAGT GCACGGTCAC CAACAGAGGC
CGTACTGTCA ACCTCAAGTC AGCCACCTGG AAGGTCTTGC ACTGCACGGG CCAGGTGAAA
GTCTACAACA ACTGCCCTCC TCACAATAGT CTGTGTGGCT ACAAGGAGCC CCTGCTGTCC
TGCCTCATCA TCATGTGTGA ACCAATCCAG CACCCATCCC ACATGGACAT CCCCCTGGAT
AGCAAGACCT TCCTGAGCCG CCACAGCATG GACATGAAGT TCACCTACTG TGATGACAGA
ATCACAGAAC TGATTGGTTA CCACCCTGAG GAGCTGCTTG GCCGCTCAGC CTATGAATTC
TACCATGCGC TAGACTCCGA GAACATGACC AAGAGTCACC AGAACTTGTG CACCAAGGGT
CAGGTAGTAA GTGGCCAGTA CCGGATGCTC GCAAAGCATG GGGGCTACGT GTGGCTGGAG
ACCCAGGGGA CGGTCATCTA CAACCCTCGC AACCTGCAGC CCCAGTGCAT CATGTGTGTC
AACTACGTCC TGAGTGAGAT TGAGAAGAAT GACGTGGTGT TCTCCATGGA CCAGACTGAA
TCCCTGTTCA AGCCCCACCT GATGGCCATG AACAGCATCT TTGATAGCAG TGGCAAGGGG
GCTGTGTCTG AGAAGAGTAA CTTCCTATTC ACCAAGCTAA AGGAGGAGCC CGAGGAGCTG
GCCCAGCTGG CTCCCACCCC AGGAGACGCC ATCATCTCTC TGGATTTCGG GAATCAGAAC
TTCGAGGAGT CCTCAGCCTA TGGCAAGGCC ATCCTGCCCC CGAGCCAGCC ATGGGCCACG
GAGTTGAGGA GCCACAGCAC CCAGAGCGAG GCTGGGAGCC TGCCTGCCTT CACCGTGCCC
CAGGCAGCTG CCCCGGGCAG CACCACCCCC AGTGCCACCA GCAGCAGCAG CAGCTGCTCC
ACGCCCAATA GCCCTGAAGA CTATTACACA TCTTTGGATA ACGACCTGAA GATTGAAGTG
ATTGAGAAGC TCTTCGCCAT GGACACAGAG GCCAAGGACC AATGCAGTAC CCAGACGGAT
TTCAATGAGC TGGACTTGGA GACACTGGCA CCCTATATCC CCATGGACGG GGAAGACTTC
CAGCTAAGCC CCATCTGCCC CGAGGAGCGG CTCTTGGCGG AGAACCCACA GTCCACCCCC
CAGCACTGCT TCAGTGCCAT GACAAACATC TTCCAGCCAC TGGCCCCTGT AGCCCCGCAC
AGTCCCTTCC TCCTGGACAA GTTTCAGCAG CAGCTGGAGA GCAAGAAGAC AGAGCCCGAG
CACCGGCCCA TGTCCTCCAT CTTCTTTGAT GCCGGAAGCA AAGCATCCCT GCCACCGTGC
TGTGGCCAGG CCAGCACCCC TCTCTCTTCC ATGGGGGGCA GATCCAATAC CCAGTGGCCC
CCAGATCCAC CATTACATTT TGGGCCCACA AAGTGGGCCG TCGGGGATCA GCGCACAGAG
TTCTTGGGAG CAGCGCCGTT GGGGCCCCCT GTCTCTCCAC CCCATGTCTC CACCTTCAAG
ACAAGGTCTG CAAAGGGTTT TGGGGCTCGA GGCCCAGACG TGCTGAGTCC GGCCATGGTA
GCCCTCTCCA ACAAGCTGAA GCTGAAGCGA CAGCTGGAGT ATGAAGAGCA AGCCTTCCAG
GACCTGAGCG GGGGGGACCC ACCTGGTGGC AGCACCTCAC ATTTGATGTG GAAACGGATG
AAGAACCTCA GGGGTGGGAG CTGCCCTTTG ATGCCGGACA AGCCACTGAG CGCAAATGTA
CCCAATGATA AGTTCACCCA AAACCCCATG AGGGGCCTGG GCCATCCCCT GAGACATCTG
CCGCTGCCAC AGCCTCCATC TGCCATCAGT CCCGGGGAGA ACAGCAAGAG CAGGTTCCCC
CCACAGTGCT ACGCCACCCA GTACCAGGAC TACAGCCTGT CGTCAGCCCA CAAGGTGTCA
GGCATGGCAA GCCGGCTGCT CGGGCCCTCA TTTGAGTCCT ACCTGCTGCC CGAACTGACC
AGATATGACT GTGAGGTGAA CGTGCCCGTG CTGGGAAGCT CCACGCTCCT GCAAGGAGGG
GACCTCCTCA GAGCCCTGGA CCAGGCCACC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57508
Clone ID Related Accession (Same CDS sequence) XM_011532698.2
Accession Version XM_011532698.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2652bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product endothelial PAS domain-containing protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532698.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGTTAGGGC TTTTTGTTTT TAAAACAGGG ACACAAAGAA GAAACAGAAG GCTGCCATAT 
GCAAGGAGTA GCTCGGAGAG GAGGAAGGAG AAGTCCCGGG ATGCTGCGCG GTGCCGGCGG
AGCAAGGAGA CGGAGGTGTT CTATGAGCTG GCCCATGAGC TGCCTCTGCC CCACAGTGTG
AGCTCCCATC TGGACAAGGC CTCCATCATG CGACTGGCAA TCAGCTTCCT GCGAACACAC
AAGCTCCTCT CCTCAGTTTG CTCTGAAAAC GAGTCCGAAG CCGAAGCTGA CCAGCAGATG
GACAACTTGT ACCTGAAAGC CTTGGAGGGT TTCATTGCCG TGGTGACCCA AGATGGCGAC
ATGATCTTTC TGTCAGAAAA CATCAGCAAG TTCATGGGAC TTACACAGGT GGAGCTAACA
GGACATAGTA TCTTTGACTT CACTCATCCC TGCGACCATG AGGAGATTCG TGAGAACCTG
AGTCTCAAAA ATGGCTCTGG TTTTGGGAAA AAAAGCAAAG ACATGTCCAC AGAGCGGGAC
TTCTTCATGA GGATGAAGTG CACGGTCACC AACAGAGGCC GTACTGTCAA CCTCAAGTCA
GCCACCTGGA AGGTCTTGCA CTGCACGGGC CAGGTGAAAG TCTACAACAA CTGCCCTCCT
CACAATAGTC TGTGTGGCTA CAAGGAGCCC CTGCTGTCCT GCCTCATCAT CATGTGTGAA
CCAATCCAGC ACCCATCCCA CATGGACATC CCCCTGGATA GCAAGACCTT CCTGAGCCGC
CACAGCATGG ACATGAAGTT CACCTACTGT GATGACAGAA TCACAGAACT GATTGGTTAC
CACCCTGAGG AGCTGCTTGG CCGCTCAGCC TATGAATTCT ACCATGCGCT AGACTCCGAG
AACATGACCA AGAGTCACCA GAACTTGTGC ACCAAGGGTC AGGTAGTAAG TGGCCAGTAC
CGGATGCTCG CAAAGCATGG GGGCTACGTG TGGCTGGAGA CCCAGGGGAC GGTCATCTAC
AACCCTCGCA ACCTGCAGCC CCAGTGCATC ATGTGTGTCA ACTACGTCCT GAGTGAGATT
GAGAAGAATG ACGTGGTGTT CTCCATGGAC CAGACTGAAT CCCTGTTCAA GCCCCACCTG
ATGGCCATGA ACAGCATCTT TGATAGCAGT GGCAAGGGGG CTGTGTCTGA GAAGAGTAAC
TTCCTATTCA CCAAGCTAAA GGAGGAGCCC GAGGAGCTGG CCCAGCTGGC TCCCACCCCA
GGAGACGCCA TCATCTCTCT GGATTTCGGG AATCAGAACT TCGAGGAGTC CTCAGCCTAT
GGCAAGGCCA TCCTGCCCCC GAGCCAGCCA TGGGCCACGG AGTTGAGGAG CCACAGCACC
CAGAGCGAGG CTGGGAGCCT GCCTGCCTTC ACCGTGCCCC AGGCAGCTGC CCCGGGCAGC
ACCACCCCCA GTGCCACCAG CAGCAGCAGC AGCTGCTCCA CGCCCAATAG CCCTGAAGAC
TATTACACAT CTTTGGATAA CGACCTGAAG ATTGAAGTGA TTGAGAAGCT CTTCGCCATG
GACACAGAGG CCAAGGACCA ATGCAGTACC CAGACGGATT TCAATGAGCT GGACTTGGAG
ACACTGGCAC CCTATATCCC CATGGACGGG GAAGACTTCC AGCTAAGCCC CATCTGCCCC
GAGGAGCGGC TCTTGGCGGA GAACCCACAG TCCACCCCCC AGCACTGCTT CAGTGCCATG
ACAAACATCT TCCAGCCACT GGCCCCTGTA GCCCCGCACA GTCCCTTCCT CCTGGACAAG
TTTCAGCAGC AGCTGGAGAG CAAGAAGACA GAGCCCGAGC ACCGGCCCAT GTCCTCCATC
TTCTTTGATG CCGGAAGCAA AGCATCCCTG CCACCGTGCT GTGGCCAGGC CAGCACCCCT
CTCTCTTCCA TGGGGGGCAG ATCCAATACC CAGTGGCCCC CAGATCCACC ATTACATTTT
GGGCCCACAA AGTGGGCCGT CGGGGATCAG CGCACAGAGT TCTTGGGAGC AGCGCCGTTG
GGGCCCCCTG TCTCTCCACC CCATGTCTCC ACCTTCAAGA CAAGGTCTGC AAAGGGTTTT
GGGGCTCGAG GCCCAGACGT GCTGAGTCCG GCCATGGTAG CCCTCTCCAA CAAGCTGAAG
CTGAAGCGAC AGCTGGAGTA TGAAGAGCAA GCCTTCCAGG ACCTGAGCGG GGGGGACCCA
CCTGGTGGCA GCACCTCACA TTTGATGTGG AAACGGATGA AGAACCTCAG GGGTGGGAGC
TGCCCTTTGA TGCCGGACAA GCCACTGAGC GCAAATGTAC CCAATGATAA GTTCACCCAA
AACCCCATGA GGGGCCTGGG CCATCCCCTG AGACATCTGC CGCTGCCACA GCCTCCATCT
GCCATCAGTC CCGGGGAGAA CAGCAAGAGC AGGTTCCCCC CACAGTGCTA CGCCACCCAG
TACCAGGACT ACAGCCTGTC GTCAGCCCAC AAGGTGTCAG GCATGGCAAG CCGGCTGCTC
GGGCCCTCAT TTGAGTCCTA CCTGCTGCCC GAACTGACCA GATATGACTG TGAGGTGAAC
GTGCCCGTGC TGGGAAGCTC CACGCTCCTG CAAGGAGGGG ACCTCCTCAG AGCCCTGGAC
CAGGCCACCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011531000.1
CDS230..2881
Misc Feature(1)302..472
Misc Feature(2)order(311..316,332..340,344..346,416..418,425..427)
Misc Feature(3)335..337
Misc Feature(4)order(353..358,365..370,374..379,428..430,437..439,449..451,455..460,470..472)
Misc Feature(5)524..709
Misc Feature(6)989..1288
Misc Feature(7)1028..1291
Misc Feature(8)order(1037..1039,1049..1051,1067..1069,1106..1117,1193..1195,1208..1210)
Misc Feature(9)order(1097..1099,1109..1111,1133..1135,1142..1147,1229..1231,1235..1237)
Misc Feature(10)1817..1915
Misc Feature(11)2765..2875
Translation

Target ORF information:

RefSeq Version XM_011532698.2
Organism Homo sapiens(Human)
Definition Homo sapiens endothelial PAS domain protein 1 (EPAS1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011532698.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGTTAGGGC TTTTTGTTTT TAAAACAGGG ACACAAAGAA GAAACAGAAG GCTGCCATAT 
GCAAGGAGTA GCTCGGAGAG GAGGAAGGAG AAGTCCCGGG ATGCTGCGCG GTGCCGGCGG
AGCAAGGAGA CGGAGGTGTT CTATGAGCTG GCCCATGAGC TGCCTCTGCC CCACAGTGTG
AGCTCCCATC TGGACAAGGC CTCCATCATG CGACTGGCAA TCAGCTTCCT GCGAACACAC
AAGCTCCTCT CCTCAGTTTG CTCTGAAAAC GAGTCCGAAG CCGAAGCTGA CCAGCAGATG
GACAACTTGT ACCTGAAAGC CTTGGAGGGT TTCATTGCCG TGGTGACCCA AGATGGCGAC
ATGATCTTTC TGTCAGAAAA CATCAGCAAG TTCATGGGAC TTACACAGGT GGAGCTAACA
GGACATAGTA TCTTTGACTT CACTCATCCC TGCGACCATG AGGAGATTCG TGAGAACCTG
AGTCTCAAAA ATGGCTCTGG TTTTGGGAAA AAAAGCAAAG ACATGTCCAC AGAGCGGGAC
TTCTTCATGA GGATGAAGTG CACGGTCACC AACAGAGGCC GTACTGTCAA CCTCAAGTCA
GCCACCTGGA AGGTCTTGCA CTGCACGGGC CAGGTGAAAG TCTACAACAA CTGCCCTCCT
CACAATAGTC TGTGTGGCTA CAAGGAGCCC CTGCTGTCCT GCCTCATCAT CATGTGTGAA
CCAATCCAGC ACCCATCCCA CATGGACATC CCCCTGGATA GCAAGACCTT CCTGAGCCGC
CACAGCATGG ACATGAAGTT CACCTACTGT GATGACAGAA TCACAGAACT GATTGGTTAC
CACCCTGAGG AGCTGCTTGG CCGCTCAGCC TATGAATTCT ACCATGCGCT AGACTCCGAG
AACATGACCA AGAGTCACCA GAACTTGTGC ACCAAGGGTC AGGTAGTAAG TGGCCAGTAC
CGGATGCTCG CAAAGCATGG GGGCTACGTG TGGCTGGAGA CCCAGGGGAC GGTCATCTAC
AACCCTCGCA ACCTGCAGCC CCAGTGCATC ATGTGTGTCA ACTACGTCCT GAGTGAGATT
GAGAAGAATG ACGTGGTGTT CTCCATGGAC CAGACTGAAT CCCTGTTCAA GCCCCACCTG
ATGGCCATGA ACAGCATCTT TGATAGCAGT GGCAAGGGGG CTGTGTCTGA GAAGAGTAAC
TTCCTATTCA CCAAGCTAAA GGAGGAGCCC GAGGAGCTGG CCCAGCTGGC TCCCACCCCA
GGAGACGCCA TCATCTCTCT GGATTTCGGG AATCAGAACT TCGAGGAGTC CTCAGCCTAT
GGCAAGGCCA TCCTGCCCCC GAGCCAGCCA TGGGCCACGG AGTTGAGGAG CCACAGCACC
CAGAGCGAGG CTGGGAGCCT GCCTGCCTTC ACCGTGCCCC AGGCAGCTGC CCCGGGCAGC
ACCACCCCCA GTGCCACCAG CAGCAGCAGC AGCTGCTCCA CGCCCAATAG CCCTGAAGAC
TATTACACAT CTTTGGATAA CGACCTGAAG ATTGAAGTGA TTGAGAAGCT CTTCGCCATG
GACACAGAGG CCAAGGACCA ATGCAGTACC CAGACGGATT TCAATGAGCT GGACTTGGAG
ACACTGGCAC CCTATATCCC CATGGACGGG GAAGACTTCC AGCTAAGCCC CATCTGCCCC
GAGGAGCGGC TCTTGGCGGA GAACCCACAG TCCACCCCCC AGCACTGCTT CAGTGCCATG
ACAAACATCT TCCAGCCACT GGCCCCTGTA GCCCCGCACA GTCCCTTCCT CCTGGACAAG
TTTCAGCAGC AGCTGGAGAG CAAGAAGACA GAGCCCGAGC ACCGGCCCAT GTCCTCCATC
TTCTTTGATG CCGGAAGCAA AGCATCCCTG CCACCGTGCT GTGGCCAGGC CAGCACCCCT
CTCTCTTCCA TGGGGGGCAG ATCCAATACC CAGTGGCCCC CAGATCCACC ATTACATTTT
GGGCCCACAA AGTGGGCCGT CGGGGATCAG CGCACAGAGT TCTTGGGAGC AGCGCCGTTG
GGGCCCCCTG TCTCTCCACC CCATGTCTCC ACCTTCAAGA CAAGGTCTGC AAAGGGTTTT
GGGGCTCGAG GCCCAGACGT GCTGAGTCCG GCCATGGTAG CCCTCTCCAA CAAGCTGAAG
CTGAAGCGAC AGCTGGAGTA TGAAGAGCAA GCCTTCCAGG ACCTGAGCGG GGGGGACCCA
CCTGGTGGCA GCACCTCACA TTTGATGTGG AAACGGATGA AGAACCTCAG GGGTGGGAGC
TGCCCTTTGA TGCCGGACAA GCCACTGAGC GCAAATGTAC CCAATGATAA GTTCACCCAA
AACCCCATGA GGGGCCTGGG CCATCCCCTG AGACATCTGC CGCTGCCACA GCCTCCATCT
GCCATCAGTC CCGGGGAGAA CAGCAAGAGC AGGTTCCCCC CACAGTGCTA CGCCACCCAG
TACCAGGACT ACAGCCTGTC GTCAGCCCAC AAGGTGTCAG GCATGGCAAG CCGGCTGCTC
GGGCCCTCAT TTGAGTCCTA CCTGCTGCCC GAACTGACCA GATATGACTG TGAGGTGAAC
GTGCCCGTGC TGGGAAGCTC CACGCTCCTG CAAGGAGGGG ACCTCCTCAG AGCCCTGGAC
CAGGCCACCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

MicroRNA-383 is a tumor suppressor in human lung cancer by targeting endothelial PAS domain-containing protein 1.
Cell biochemistry and function34(8)613-619(2016 Dec)
Ma H,Liu B,Wang S,Liu J


book

Correlation of HIF-1α/HIF-2α expression with FDG uptake in lung adenocarcinoma.
Annals of nuclear medicine30(10)708-715(2016 Dec)
Higashi K,Yamagishi T,Ueda Y,Ishigaki Y,Shimasaki M,Nakamura Y,Oguchi M,Takegami T,Sagawa M,Tonami H


book

Combined over-expression of the hypoxia-inducible factor 2α gene and its long non-coding RNA predicts unfavorable prognosis of patients with osteosarcoma.
Pathology, research and practice212(10)861-866(2016 Oct)
Li W,He X,Xue R,Zhang Y,Zhang X,Lu J,Zhang Z,Xue L


book

MBD3 mediates epigenetic regulation on EPAS1 promoter in cancer.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(10)13455-13467(2016 Oct)
Cui J,Duan B,Zhao X,Chen Y,Sun S,Deng W,Zhang Y,Du J,Chen Y,Gu L


book

HIF1A and EPAS1 mRNA and protein expression during in vitro culture of human primary term cytotrophoblasts and effect of oxygen tension on their expression.
Reproductive biology16(3)203-211(2016 Sep)
Depoix CL,Flabat O,Debiève F,Hubinont C


 
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