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VMA21 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol VMA21
Entrez Gene ID 203547
Full Name VMA21, vacuolar ATPase assembly factor
Synonyms MEAX,XMEA
General protein information
Preferred Names

VMA21, vacuolar ATPase assembly factor

Names

vacuolar ATPase assembly integral membrane protein VMA21
VMA21 vacuolar H+-ATPase homolog
myopathy with excessive autophagy protein

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

X

Xq28

Summary This gene encodes a chaperone for assembly of lysosomal vacuolar ATPase.[provided by RefSeq, Jul 2012].
Disorder MIM:

300913


mRNA and Protein(s)

mRNA Protein Name
NM_001017980.3 NP_001017980.1 vacuolar ATPase assembly integral membrane protein VMA21
XM_011531125.2 XP_011529427.1 vacuolar ATPase assembly integral membrane protein VMA21 isoform X1



Bos taurus (cattle) VMA21 NP_001075085.1
Pan troglodytes (chimpanzee) VMA21 XP_001136167.1
Homo sapiens (human) VMA21 NP_001017980.1
Canis lupus familiaris (dog) VMA21 NP_001239241.1


Related articles in PubMed

X-linked myopathy with excessive autophagy: a failure of self-eating.
Dowling JJ, Moore SA, Kalimo H, Minassian BA
Acta neuropathologica129(3)383-90(2015 Mar)

Elevated urinary β2 microglobulin in the first identified Japanese family afflicted by X-linked myopathy with excessive autophagy.
Kurashige T, Takahashi T, Yamazaki Y, Nagano Y, Kondo K, Nakamura T, Yamawaki T, Tsuburaya R, Hayashi YK, Nonaka I, Nishino I, Matsumoto M
Neuromuscular disorders : NMD23(11)911-6(2013 Nov)

VMA21 deficiency prevents vacuolar ATPase assembly and causes autophagic vacuolar myopathy.
Ramachandran N, Munteanu I, Wang P, Ruggieri A, Rilstone JJ, Israelian N, Naranian T, Paroutis P, Guo R, Ren ZP, Nishino I, Chabrol B, Pellissier JF, Minetti C, Udd B, Fardeau M, Tailor CS, Mahuran DJ, Kissel JT, Kalimo H, Levy N, Manolson MF, Ackerley CA, Minassian BA
Acta neuropathologica125(3)439-57(2013 Mar)

Impaired autophagy in sporadic inclusion-body myositis and in endoplasmic reticulum stress-provoked cultured human muscle fibers.
Nogalska A, D'Agostino C, Terracciano C, Engel WK, Askanas V
The American journal of pathology177(3)1377-87(2010 Sep)

VMA21 deficiency: a case of myocyte indigestion.
Hirano M, DiMauro S
Cell137(2)213-5(2009 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following VMA21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VMA21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu23612 NM_001017980.3
Latest version!
Homo sapiens VMA21, vacuolar ATPase assembly factor (VMA21), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu77854 XM_011531125.2
Latest version!
Homo sapiens VMA21 vacuolar H+-ATPase homolog (S. cerevisiae) (VMA21), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00

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Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu23612
Clone ID Related Accession (Same CDS sequence) NM_001017980.3
Accession Version NM_001017980.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 306bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-30
Organism Homo sapiens(Human)
Product vacuolar ATPase assembly integral membrane protein VMA21
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA224893.1, AL833596.1 and BC110800.1. This sequence is a reference standard in the RefSeqGene project. On Oct 3, 2009 this sequence version replaced NM_001017980.2. ##Evidence-Data-START## Transcript exon combination :: AL833596.1, SRR1660805.252891.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGGAGCGCC CGGATAAGGC GGCGCTGAAC GCACTGCAGC CTCCTGAGTT CAGAAATGAA 
AGCTCATTAG CATCTACACT GAAGACGCTC CTGTTCTTCA CAGCTTTAAT GATCACTGTT
CCTATTGGGT TATATTTCAC AACTAAATCT TACATATTTG AAGGCGCCCT TGGGATGTCC
AATAGGGACA GCTATTTTTA CGCTGCTATT GTTGCAGTGG TCGCCGTCCA TGTGGTGCTG
GCCCTCTTTG TGTATGTGGC CTGGAATGAA GGCTCACGAC AGTGGCGTGA AGGCAAACAG
GATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001017980.1
CDS125..430
Misc Feature(1)197..388
Misc Feature(2)200..262
Misc Feature(3)320..382
Exon (1)1..177
Exon (2)178..287
Exon (3)288..4736
Translation

Target ORF information:

RefSeq Version NM_001017980.3
Organism Homo sapiens(Human)
Definition Homo sapiens VMA21, vacuolar ATPase assembly factor (VMA21), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001017980.3

ORF Insert Sequence:

1
61
121
181
241
301
ATGGAGCGCC CGGATAAGGC GGCGCTGAAC GCACTGCAGC CTCCTGAGTT CAGAAATGAA 
AGCTCATTAG CATCTACACT GAAGACGCTC CTGTTCTTCA CAGCTTTAAT GATCACTGTT
CCTATTGGGT TATATTTCAC AACTAAATCT TACATATTTG AAGGCGCCCT TGGGATGTCC
AATAGGGACA GCTATTTTTA CGCTGCTATT GTTGCAGTGG TCGCCGTCCA TGTGGTGCTG
GCCCTCTTTG TGTATGTGGC CTGGAATGAA GGCTCACGAC AGTGGCGTGA AGGCAAACAG
GATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu77854
Clone ID Related Accession (Same CDS sequence) XM_011531125.2
Accession Version XM_011531125.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 471bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product vacuolar ATPase assembly integral membrane protein VMA21 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011531125.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
ATGCTCGGGT CTCCTTGCGG CCCCCAGCTC AGCGACCGAG ACGCAGACGA GGACCAGTGT 
TCACGCGAGT TCAGGGGGCG GCGTAGCCGC CGCCCGCCCA GGAGGACCAT GTTGCGCGGC
AAGTCCCGGC TCAACGTGGA GTGGCTGGGC TACTCGCCAG GCCTGCTCCT CGAGCACAGG
CCCCTCCTGG CAGGGCGCAC GCCGCGGAGC CACCGCCGAA ATGAAAGCTC ATTAGCATCT
ACACTGAAGA CGCTCCTGTT CTTCACAGCT TTAATGATCA CTGTTCCTAT TGGGTTATAT
TTCACAACTA AATCTTACAT ATTTGAAGGC GCCCTTGGGA TGTCCAATAG GGACAGCTAT
TTTTACGCTG CTATTGTTGC AGTGGTCGCC GTCCATGTGG TGCTGGCCCT CTTTGTGTAT
GTGGCCTGGA ATGAAGGCTC ACGACAGTGG CGTGAAGGCA AACAGGATTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011529427.1
CDS286..756
Misc Feature(1)523..>651
Translation

Target ORF information:

RefSeq Version XM_011531125.2
Organism Homo sapiens(Human)
Definition Homo sapiens VMA21 vacuolar H+-ATPase homolog (S. cerevisiae) (VMA21), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011531125.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGCTCGGGT CTCCTTGCGG CCCCCAGCTC AGCGACCGAG ACGCAGACGA GGACCAGTGT 
TCACGCGAGT TCAGGGGGCG GCGTAGCCGC CGCCCGCCCA GGAGGACCAT GTTGCGCGGC
AAGTCCCGGC TCAACGTGGA GTGGCTGGGC TACTCGCCAG GCCTGCTCCT CGAGCACAGG
CCCCTCCTGG CAGGGCGCAC GCCGCGGAGC CACCGCCGAA ATGAAAGCTC ATTAGCATCT
ACACTGAAGA CGCTCCTGTT CTTCACAGCT TTAATGATCA CTGTTCCTAT TGGGTTATAT
TTCACAACTA AATCTTACAT ATTTGAAGGC GCCCTTGGGA TGTCCAATAG GGACAGCTAT
TTTTACGCTG CTATTGTTGC AGTGGTCGCC GTCCATGTGG TGCTGGCCCT CTTTGTGTAT
GTGGCCTGGA ATGAAGGCTC ACGACAGTGG CGTGAAGGCA AACAGGATTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

X-linked myopathy with excessive autophagy: a failure of self-eating.
Acta neuropathologica129(3)383-90(2015 Mar)
Dowling JJ,Moore SA,Kalimo H,Minassian BA


book

Elevated urinary β2 microglobulin in the first identified Japanese family afflicted by X-linked myopathy with excessive autophagy.
Neuromuscular disorders : NMD23(11)911-6(2013 Nov)
Kurashige T,Takahashi T,Yamazaki Y,Nagano Y,Kondo K,Nakamura T,Yamawaki T,Tsuburaya R,Hayashi YK,Nonaka I,Nishino I,Matsumoto M


book

VMA21 deficiency prevents vacuolar ATPase assembly and causes autophagic vacuolar myopathy.
Acta neuropathologica125(3)439-57(2013 Mar)
Ramachandran N,Munteanu I,Wang P,Ruggieri A,Rilstone JJ,Israelian N,Naranian T,Paroutis P,Guo R,Ren ZP,Nishino I,Chabrol B,Pellissier JF,Minetti C,Udd B,Fardeau M,Tailor CS,Mahuran DJ,Kissel JT,Kalimo H,Levy N,Manolson MF,Ackerley CA,Minassian BA


book

Impaired autophagy in sporadic inclusion-body myositis and in endoplasmic reticulum stress-provoked cultured human muscle fibers.
The American journal of pathology177(3)1377-87(2010 Sep)
Nogalska A,D'Agostino C,Terracciano C,Engel WK,Askanas V


book

VMA21 deficiency: a case of myocyte indigestion.
Cell137(2)213-5(2009 Apr)
Hirano M,DiMauro S


 
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