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EPB42 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol EPB42
Entrez Gene ID 2038
Full Name erythrocyte membrane protein band 4.2
Synonyms PA,SPH5
General protein information
Preferred Names

erythrocyte membrane protein band 4.2

Names

erythrocyte membrane protein band 4.2
P4.2
erythrocyte protein 4.2
erythrocyte surface protein band 4.2

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

15

15q15.2

Summary Erythrocyte membrane protein band 4.2 is an ATP-binding protein which may regulate the association of protein 3 with ankyrin. It probably has a role in erythrocyte shape and mechanical property regulation. Mutations in the EPB42 gene are associated with recessive spherocytic elliptocytosis and recessively transmitted hereditary hemolytic anemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:

177070

Disorder Html: Spherocytosis, hereditary, type 5, 612690 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_011521351.2 XP_011519653.1 erythrocyte membrane protein band 4.2 isoform X1
NM_001114134.1 NP_001107606.1 erythrocyte membrane protein band 4.2 isoform 2
XM_011521350.2 XP_011519652.1 erythrocyte membrane protein band 4.2 isoform X1
NM_000119.2 NP_000110.2 erythrocyte membrane protein band 4.2 isoform 1
XM_011521349.2 XP_011519651.1 erythrocyte membrane protein band 4.2 isoform X1
XM_011521353.2 XP_011519655.1 erythrocyte membrane protein band 4.2 isoform X3
XM_011521352.2 XP_011519654.1 erythrocyte membrane protein band 4.2 isoform X2
XM_011521354.2 XP_011519656.1 erythrocyte membrane protein band 4.2 isoform X5
XM_005254225.1 XP_005254282.1 erythrocyte membrane protein band 4.2 isoform X4



Mus musculus (house mouse) Epb4.2 NP_038541.1
Rattus norvegicus (Norway rat) Epb42 NP_001102060.1
Bos taurus (cattle) EPB42 NP_776737.1
Macaca mulatta (rhesus monkey) LOC709427 XP_002808491.1
Xenopus tropicalis (tropical clawed frog) epb42 XP_002935587.2
Homo sapiens (human) EPB42 NP_000110.2
Pan troglodytes (chimpanzee) EPB42 XP_510346.2
Canis lupus familiaris (dog) EPB42 XP_005638380.1
Gallus gallus (chicken) EPB42 XP_417393.2


Related articles in PubMed

Protein 4.2 interaction with hereditary spherocytosis mutants of the cytoplasmic domain of human anion exchanger 1.
Bustos SP, Reithmeier RA
The Biochemical journal433(2)313-22(2011 Jan)

Protein 4.2 binds to the carboxyl-terminal EF-hands of erythroid alpha-spectrin in a calcium- and calmodulin-dependent manner.
Korsgren C, Peters LL, Lux SE
The Journal of biological chemistry285(7)4757-70(2010 Feb)

Protein 4.2: a complex linker.
Satchwell TJ, Shoemark DK, Sessions RB, Toye AM
Blood cells, molecules & diseases42(3)201-10(2009 May-Jun)

Associations of protein 4.2 with band 3 and ankyrin.
Su Y, Ding Y, Jiang M, Jiang W, Hu X, Zhang Z
Molecular and cellular biochemistry289(1-2)159-66(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study shows that cytoplasmic hereditary spherocytosis mutants cause impaired binding of protein 4.2 to AE1, leaving protein 4.2 susceptible to loss during erythrocyte development
Title: Protein 4.2 interaction with hereditary spherocytosis mutants of the cytoplasmic domain of human anion exchanger 1.

Data suggest that one or both of proteins 4.1 and 4.2 cause a portion of band 3 to localize near the spectrin-actin junctions and provide another point of attachment between the membrane skeleton and the lipid bilayer.
Title: Protein 4.2 binds to the carboxyl-terminal EF-hands of erythroid alpha-spectrin in a calcium- and calmodulin-dependent manner.

Current understanding of protein 4.2, its known interactions & implications of protein 4.2 deficiency are reviewed. A new speculative "open" homology structure for the the active, membrane associated form is proposed. Review.
Title: Protein 4.2: a complex linker.

The interactions of three protein 4.2-derived recombinant proteins with CDB3 and ankyrin were investigated by using Far-Western blot and pull-down assay.
Title: Associations of protein 4.2 with band 3 and ankyrin.

protein 4.2 strongly influences CD47 levels as well as the extent of membrane skeleton attachment in erythrocytes
Title: Protein 4.2 is critical to CD47-membrane skeleton attachment in human red cells.

The following EPB42 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EPB42 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu17621 NM_001114134.1
Latest version!
Homo sapiens erythrocyte membrane protein band 4.2 (EPB42), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$279.50-$391.30
$559.00
OHu57540 XM_011521354.2
Latest version!
Homo sapiens erythrocyte membrane protein band 4.2 (EPB42), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $307.30
$439.00
OHu48168 XM_005254225.1
Latest version!
Homo sapiens erythrocyte membrane protein band 4.2 (EPB42), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu18437 NM_000119.2
Latest version!
Homo sapiens erythrocyte membrane protein band 4.2 (EPB42), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu18437 XM_011521350.2
Latest version!
Homo sapiens erythrocyte membrane protein band 4.2 (EPB42), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu18437 XM_011521349.2
Latest version!
Homo sapiens erythrocyte membrane protein band 4.2 (EPB42), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu18437 XM_011521351.2
Latest version!
Homo sapiens erythrocyte membrane protein band 4.2 (EPB42), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu57538 XM_011521352.2
Latest version!
Homo sapiens erythrocyte membrane protein band 4.2 (EPB42), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu57539 XM_011521353.2
Latest version!
Homo sapiens erythrocyte membrane protein band 4.2 (EPB42), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu17621
Clone ID Related Accession (Same CDS sequence) NM_001114134.1
Accession Version NM_001114134.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2076bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-10-06
Organism Homo sapiens(Human)
Product erythrocyte membrane protein band 4.2 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DW451374.1, BC096094.2 and BU741892.1. Transcript Variant: This variant (2) has an alternate splice site in the 5' region, as compared to variant 1. The resulting isoform 2 lacks an internal segment in the N-terminal region, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M30646.1, M29399.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2142680 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGGACAGG CCCTGGGTAT CAAGAGCTGT GACTTTCAGG CAGCAAGAAA CAATGAGGAG 
CACCACACCA AGGCCCTCAG CTCCCGGCGC CTCTTTGTGA GGAGGGGGCA GCCCTTCACC
ATCATCCTGT ACTTCCGCGC TCCAGTCCGT GCATTTCTGC CTGCCCTGAA GAAGGTGGCC
CTCACTGCAC AAACTGGAGA GCAGCCTTCC AAGATCAACA GGACCCAAGC CACATTCCCA
ATTTCCAGTC TGGGGGACCG AAAGTGGTGG AGTGCAGTGG TGGAGGAGAG AGATGCCCAG
TCCTGGACCA TCTCTGTGAC CACACCTGCG GACGCTGTCA TTGGCCACTA CTCGCTTCTG
CTGCAGGTCT CAGGCAGGAA GCAACTCCTC TTGGGTCAGT TCACACTGCT TTTTAACCCC
TGGAATAGAG AGGATGCTGT GTTCCTGAAG AATGAGGCTC AGCGCATGGA GTACTTGTTG
AACCAGAATG GTCTCATCTA CCTGGGTACA GCTGACTGCA TCCAGGCAGA GTCCTGGGAC
TTTGGCCAGT TCGAGGGGGA TGTCATTGAC CTCAGCCTGC GCTTGCTGAG CAAGGACAAG
CAGGTAGAGA AGTGGAGCCA GCCGGTGCAC GTGGCCCGTG TGTTGGGTGC CTTGCTGCAT
TTTCTCAAGG AGCAGAGGGT CCTGCCCACC CCGCAGACCC AGGCCACCCA GGAAGGGGCC
TTGCTGAACA AGCGCCGGGG CAGCGTGCCC ATCCTGCGGC AGTGGCTCAC CGGCCGAGGC
CGACCTGTGT ATGATGGCCA GGCCTGGGTG TTGGCTGCTG TTGCTTGCAC AGTGCTGCGA
TGCCTGGGAA TCCCTGCCCG CGTGGTGACC ACGTTTGCCT CAGCACAGGG CACCGGTGGG
CGTCTTCTCA TAGATGAATA CTATAATGAG GAGGGACTTC AGAACGGAGA AGGCCAGAGA
GGCAGAATCT GGATCTTCCA GACTTCCACA GAGTGCTGGA TGACGCGGCC TGCCTTGCCC
CAGGGTTATG ATGGATGGCA GATTCTGCAC CCAAGTGCTC CTAATGGAGG TGGAGTCCTG
GGGTCCTGTG ATCTGGTGCC GGTCAGAGCA GTCAAGGAGG GGACGCTGGG GCTGACCCCA
GCAGTGTCAG ACCTTTTTGC TGCCATAAAT GCCTCATGTG TGGTCTGGAA GTGCTGTGAG
GATGGGACAC TGGAGTTGAC TGACTCCAAC ACAAAGTATG TTGGCAACAA CATCAGCACC
AAGGGTGTGG GCAGTGACCG CTGCGAGGAC ATCACTCAGA ACTACAAGTA TCCTGAAGGG
TCTCTTCAGG AAAAAGAGGT GCTGGAGAGA GTCGAGAAAG AGAAAATGGA ACGTGAGAAA
GACAACGGCA TCCGTCCTCC CAGTCTCGAG ACTGCCAGTC CTCTGTACCT GCTCTTGAAA
GCACCCAGCT CCCTACCCCT GAGAGGGGAT GCCCAGATCT CAGTGACGCT GGTTAATCAC
AGTGAGCAGG AGAAGGCAGT GCAGCTGGCA ATTGGGGTCC AGGCTGTACA CTACAACGGT
GTCCTTGCTG CCAAGCTCTG GAGGAAGAAG CTGCACCTCA CGCTCAGTGC CAACCTGGAA
AAGATAATAA CCATCGGCCT GTTCTTCTCC AATTTTGAGC GAAACCCACC CGAGAACACC
TTCCTTAGAC TCACCGCCAT GGCAACACAC TCTGAATCCA ACCTTAGCTG CTTTGCTCAG
GAAGACATTG CCATTTGTAG ACCACACCTT GCCATCAAGA TGCCAGAGAA AGCAGAGCAG
TATCAACCCC TCACAGCCTC AGTCAGCCTC CAGAACTCCC TAGATGCCCC CATGGAGGAC
TGTGTGATCT CCATCCTGGG AAGGGGGCTC ATTCACAGAG AGAGGAGCTA CAGATTCCGT
TCAGTGTGGC CTGAAAACAC CATGTGTGCC AAGTTCCAGT TCACGCCAAC ACATGTGGGG
CTCCAGAGAC TCACTGTGGA AGTGGACTGC AACATGTTCC AGAACCTAAC CAACTATAAA
AGCGTCACCG TGGTAGCCCC TGAACTATCA GCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001107606.1
CDS301..2376
Misc Feature(1)280..282
Misc Feature(2)319..669
Misc Feature(3)391..417
Misc Feature(4)1042..1044
Misc Feature(5)1081..1356
Misc Feature(6)1723..2040
Misc Feature(7)2062..2358
Exon (1)1..310
Exon (2)311..496
Exon (3)497..730
Exon (4)731..849
Exon (5)850..954
Exon (6)955..1132
Exon (7)1133..1271
Exon (8)1272..1375
Exon (9)1376..1618
Exon (10)1619..1918
Exon (11)1919..2079
Exon (12)2080..2213
Exon (13)2214..2451
Translation

Target ORF information:

RefSeq Version NM_001114134.1
Organism Homo sapiens(Human)
Definition Homo sapiens erythrocyte membrane protein band 4.2 (EPB42), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001114134.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGGACAGG CCCTGGGTAT CAAGAGCTGT GACTTTCAGG CAGCAAGAAA CAATGAGGAG 
CACCACACCA AGGCCCTCAG CTCCCGGCGC CTCTTTGTGA GGAGGGGGCA GCCCTTCACC
ATCATCCTGT ACTTCCGCGC TCCAGTCCGT GCATTTCTGC CTGCCCTGAA GAAGGTGGCC
CTCACTGCAC AAACTGGAGA GCAGCCTTCC AAGATCAACA GGACCCAAGC CACATTCCCA
ATTTCCAGTC TGGGGGACCG AAAGTGGTGG AGTGCAGTGG TGGAGGAGAG AGATGCCCAG
TCCTGGACCA TCTCTGTGAC CACACCTGCG GACGCTGTCA TTGGCCACTA CTCGCTTCTG
CTGCAGGTCT CAGGCAGGAA GCAACTCCTC TTGGGTCAGT TCACACTGCT TTTTAACCCC
TGGAATAGAG AGGATGCTGT GTTCCTGAAG AATGAGGCTC AGCGCATGGA GTACTTGTTG
AACCAGAATG GTCTCATCTA CCTGGGTACA GCTGACTGCA TCCAGGCAGA GTCCTGGGAC
TTTGGCCAGT TCGAGGGGGA TGTCATTGAC CTCAGCCTGC GCTTGCTGAG CAAGGACAAG
CAGGTAGAGA AGTGGAGCCA GCCGGTGCAC GTGGCCCGTG TGTTGGGTGC CTTGCTGCAT
TTTCTCAAGG AGCAGAGGGT CCTGCCCACC CCGCAGACCC AGGCCACCCA GGAAGGGGCC
TTGCTGAACA AGCGCCGGGG CAGCGTGCCC ATCCTGCGGC AGTGGCTCAC CGGCCGAGGC
CGACCTGTGT ATGATGGCCA GGCCTGGGTG TTGGCTGCTG TTGCTTGCAC AGTGCTGCGA
TGCCTGGGAA TCCCTGCCCG CGTGGTGACC ACGTTTGCCT CAGCACAGGG CACCGGTGGG
CGTCTTCTCA TAGATGAATA CTATAATGAG GAGGGACTTC AGAACGGAGA AGGCCAGAGA
GGCAGAATCT GGATCTTCCA GACTTCCACA GAGTGCTGGA TGACGCGGCC TGCCTTGCCC
CAGGGTTATG ATGGATGGCA GATTCTGCAC CCAAGTGCTC CTAATGGAGG TGGAGTCCTG
GGGTCCTGTG ATCTGGTGCC GGTCAGAGCA GTCAAGGAGG GGACGCTGGG GCTGACCCCA
GCAGTGTCAG ACCTTTTTGC TGCCATAAAT GCCTCATGTG TGGTCTGGAA GTGCTGTGAG
GATGGGACAC TGGAGTTGAC TGACTCCAAC ACAAAGTATG TTGGCAACAA CATCAGCACC
AAGGGTGTGG GCAGTGACCG CTGCGAGGAC ATCACTCAGA ACTACAAGTA TCCTGAAGGG
TCTCTTCAGG AAAAAGAGGT GCTGGAGAGA GTCGAGAAAG AGAAAATGGA ACGTGAGAAA
GACAACGGCA TCCGTCCTCC CAGTCTCGAG ACTGCCAGTC CTCTGTACCT GCTCTTGAAA
GCACCCAGCT CCCTACCCCT GAGAGGGGAT GCCCAGATCT CAGTGACGCT GGTTAATCAC
AGTGAGCAGG AGAAGGCAGT GCAGCTGGCA ATTGGGGTCC AGGCTGTACA CTACAACGGT
GTCCTTGCTG CCAAGCTCTG GAGGAAGAAG CTGCACCTCA CGCTCAGTGC CAACCTGGAA
AAGATAATAA CCATCGGCCT GTTCTTCTCC AATTTTGAGC GAAACCCACC CGAGAACACC
TTCCTTAGAC TCACCGCCAT GGCAACACAC TCTGAATCCA ACCTTAGCTG CTTTGCTCAG
GAAGACATTG CCATTTGTAG ACCACACCTT GCCATCAAGA TGCCAGAGAA AGCAGAGCAG
TATCAACCCC TCACAGCCTC AGTCAGCCTC CAGAACTCCC TAGATGCCCC CATGGAGGAC
TGTGTGATCT CCATCCTGGG AAGGGGGCTC ATTCACAGAG AGAGGAGCTA CAGATTCCGT
TCAGTGTGGC CTGAAAACAC CATGTGTGCC AAGTTCCAGT TCACGCCAAC ACATGTGGGG
CTCCAGAGAC TCACTGTGGA AGTGGACTGC AACATGTTCC AGAACCTAAC CAACTATAAA
AGCGTCACCG TGGTAGCCCC TGAACTATCA GCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57540
Clone ID Related Accession (Same CDS sequence) XM_011521354.2
Accession Version XM_011521354.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1611bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product erythrocyte membrane protein band 4.2 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521354.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGAGTACT TGTTGAACCA GAATGGTCTC ATCTACCTGG GTACAGCTGA CTGCATCCAG 
GCAGAGTCCT GGGACTTTGG CCAGTTCGAG GGGGATGTCA TTGACCTCAG CCTGCGCTTG
CTGAGCAAGG ACAAGCAGGT AGAGAAGTGG AGCCAGCCGG TGCACGTGGC CCGTGTGTTG
GGTGCCTTGC TGCATTTTCT CAAGGAGCAG AGGGTCCTGC CCACCCCGCA GACCCAGGCC
ACCCAGGAAG GGGCCTTGCT GAACAAGCGC CGGGGCAGCG TGCCCATCCT GCGGCAGTGG
CTCACCGGCC GAGGCCGACC TGTGTATGAT GGCCAGGCCT GGGTGTTGGC TGCTGTTGCT
TGCACAGTGC TGCGATGCCT GGGAATCCCT GCCCGCGTGG TGACCACGTT TGCCTCAGCA
CAGGGCACCG GTGGGCGTCT TCTCATAGAT GAATACTATA ATGAGGAGGG ACTTCAGAAC
GGAGAAGGCC AGAGAGGCAG AATCTGGATC TTCCAGACTT CCACAGAGTG CTGGATGACG
CGGCCTGCCT TGCCCCAGGG TTATGATGGA TGGCAGATTC TGCACCCAAG TGCTCCTAAT
GGAGGTGGAG TCCTGGGGTC CTGTGATCTG GTGCCGGTCA GAGCAGTCAA GGAGGGGACG
CTGGGGCTGA CCCCAGCAGT GTCAGACCTT TTTGCTGCCA TAAATGCCTC ATGTGTGGTC
TGGAAGTGCT GTGAGGATGG GACACTGGAG TTGACTGACT CCAACACAAA GTATGTTGGC
AACAACATCA GCACCAAGGG TGTGGGCAGT GACCGCTGCG AGGACATCAC TCAGAACTAC
AAGTATCCTG AAGGGTCTCT TCAGGAAAAA GAGGTGCTGG AGAGAGTCGA GAAAGAGAAA
ATGGAACGTG AGAAAGACAA CGGCATCCGT CCTCCCAGTC TCGAGACTGC CAGTCCTCTG
TACCTGCTCT TGAAAGCACC CAGCTCCCTA CCCCTGAGAG GGGATGCCCA GATCTCAGTG
ACGCTGGTTA ATCACAGTGA GCAGGAGAAG GCAGTGCAGC TGGCAATTGG GGTCCAGGCT
GTACACTACA ACGGTGTCCT TGCTGCCAAG CTCTGGAGGA AGAAGCTGCA CCTCACGCTC
AGTGCCAACC TGGAAAAGAT AATAACCATC GGCCTGTTCT TCTCCAATTT TGAGCGAAAC
CCACCCGAGA ACACCTTCCT TAGACTCACC GCCATGGCAA CACACTCTGA ATCCAACCTT
AGCTGCTTTG CTCAGGAAGA CATTGCCATT TGTAGACCAC ACCTTGCCAT CAAGATGCCA
GAGAAAGCAG AGCAGTATCA ACCCCTCACA GCCTCAGTCA GCCTCCAGAA CTCCCTAGAT
GCCCCCATGG AGGACTGTGT GATCTCCATC CTGGGAAGGG GGCTCATTCA CAGAGAGAGG
AGCTACAGAT TCCGTTCAGT GTGGCCTGAA AACACCATGT GTGCCAAGTT CCAGTTCACG
CCAACACATG TGGGGCTCCA GAGACTCACT GTGGAAGTGG ACTGCAACAT GTTCCAGAAC
CTAACCAACT ATAAAAGCGT CACCGTGGTA GCCCCTGAAC TATCAGCTTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011519656.1
CDS170..1780
Misc Feature(1)485..760
Misc Feature(2)1127..1444
Misc Feature(3)1466..1762
Translation

Target ORF information:

RefSeq Version XM_011521354.2
Organism Homo sapiens(Human)
Definition Homo sapiens erythrocyte membrane protein band 4.2 (EPB42), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011521354.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGAGTACT TGTTGAACCA GAATGGTCTC ATCTACCTGG GTACAGCTGA CTGCATCCAG 
GCAGAGTCCT GGGACTTTGG CCAGTTCGAG GGGGATGTCA TTGACCTCAG CCTGCGCTTG
CTGAGCAAGG ACAAGCAGGT AGAGAAGTGG AGCCAGCCGG TGCACGTGGC CCGTGTGTTG
GGTGCCTTGC TGCATTTTCT CAAGGAGCAG AGGGTCCTGC CCACCCCGCA GACCCAGGCC
ACCCAGGAAG GGGCCTTGCT GAACAAGCGC CGGGGCAGCG TGCCCATCCT GCGGCAGTGG
CTCACCGGCC GAGGCCGACC TGTGTATGAT GGCCAGGCCT GGGTGTTGGC TGCTGTTGCT
TGCACAGTGC TGCGATGCCT GGGAATCCCT GCCCGCGTGG TGACCACGTT TGCCTCAGCA
CAGGGCACCG GTGGGCGTCT TCTCATAGAT GAATACTATA ATGAGGAGGG ACTTCAGAAC
GGAGAAGGCC AGAGAGGCAG AATCTGGATC TTCCAGACTT CCACAGAGTG CTGGATGACG
CGGCCTGCCT TGCCCCAGGG TTATGATGGA TGGCAGATTC TGCACCCAAG TGCTCCTAAT
GGAGGTGGAG TCCTGGGGTC CTGTGATCTG GTGCCGGTCA GAGCAGTCAA GGAGGGGACG
CTGGGGCTGA CCCCAGCAGT GTCAGACCTT TTTGCTGCCA TAAATGCCTC ATGTGTGGTC
TGGAAGTGCT GTGAGGATGG GACACTGGAG TTGACTGACT CCAACACAAA GTATGTTGGC
AACAACATCA GCACCAAGGG TGTGGGCAGT GACCGCTGCG AGGACATCAC TCAGAACTAC
AAGTATCCTG AAGGGTCTCT TCAGGAAAAA GAGGTGCTGG AGAGAGTCGA GAAAGAGAAA
ATGGAACGTG AGAAAGACAA CGGCATCCGT CCTCCCAGTC TCGAGACTGC CAGTCCTCTG
TACCTGCTCT TGAAAGCACC CAGCTCCCTA CCCCTGAGAG GGGATGCCCA GATCTCAGTG
ACGCTGGTTA ATCACAGTGA GCAGGAGAAG GCAGTGCAGC TGGCAATTGG GGTCCAGGCT
GTACACTACA ACGGTGTCCT TGCTGCCAAG CTCTGGAGGA AGAAGCTGCA CCTCACGCTC
AGTGCCAACC TGGAAAAGAT AATAACCATC GGCCTGTTCT TCTCCAATTT TGAGCGAAAC
CCACCCGAGA ACACCTTCCT TAGACTCACC GCCATGGCAA CACACTCTGA ATCCAACCTT
AGCTGCTTTG CTCAGGAAGA CATTGCCATT TGTAGACCAC ACCTTGCCAT CAAGATGCCA
GAGAAAGCAG AGCAGTATCA ACCCCTCACA GCCTCAGTCA GCCTCCAGAA CTCCCTAGAT
GCCCCCATGG AGGACTGTGT GATCTCCATC CTGGGAAGGG GGCTCATTCA CAGAGAGAGG
AGCTACAGAT TCCGTTCAGT GTGGCCTGAA AACACCATGT GTGCCAAGTT CCAGTTCACG
CCAACACATG TGGGGCTCCA GAGACTCACT GTGGAAGTGG ACTGCAACAT GTTCCAGAAC
CTAACCAACT ATAAAAGCGT CACCGTGGTA GCCCCTGAAC TATCAGCTTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu48168
Clone ID Related Accession (Same CDS sequence) XM_005254225.1
Accession Version XM_005254225.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1971bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product erythrocyte membrane protein band 4.2 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGGACAGG CCCTGGGTAT CAAGAGCTGT GACTTTCAGG CAGCAAGAAA CAATGAGGAG 
CACCACACCA AGGCCCTCAG CTCCCGGCGC CTCTTTGTGA GGAGGGGGCA GCCCTTCACC
ATCATCCTGT ACTTCCGCGC TCCAGTCCGT GCATTTCTGC CTGCCCTGAA GAAGGTGGCC
CTCACTGCAC AAACTGGAGA GCAGCCTTCC AAGATCAACA GGACCCAAGC CACATTCCCA
ATTTCCAGTC TGGGGGACCG AAAGTGGTGG AGTGCAGTGG TGGAGGAGAG AGATGCCCAG
TCCTGGACCA TCTCTGTGAC CACACCTGCG GACGCTGTCA TTGGCCACTA CTCGCTTCTG
CTGCAGGTCT CAGGCAGGAA GCAACTCCTC TTGGGTCAGT TCACACTGCT TTTTAACCCC
TGGAATAGAG AGGATGCTGT GTTCCTGAAG AATGAGGCTC AGCGCATGGA GTACTTGTTG
AACCAGAATG GTCTCATCTA CCTGGGTACA GCTGACTGCA TCCAGGCAGA GTCCTGGGAC
TTTGGCCAGC TGCATTTTCT CAAGGAGCAG AGGGTCCTGC CCACCCCGCA GACCCAGGCC
ACCCAGGAAG GGGCCTTGCT GAACAAGCGC CGGGGCAGCG TGCCCATCCT GCGGCAGTGG
CTCACCGGCC GAGGCCGACC TGTGTATGAT GGCCAGGCCT GGGTGTTGGC TGCTGTTGCT
TGCACAGTGC TGCGATGCCT GGGAATCCCT GCCCGCGTGG TGACCACGTT TGCCTCAGCA
CAGGGCACCG GTGGGCGTCT TCTCATAGAT GAATACTATA ATGAGGAGGG ACTTCAGAAC
GGAGAAGGCC AGAGAGGCAG AATCTGGATC TTCCAGACTT CCACAGAGTG CTGGATGACG
CGGCCTGCCT TGCCCCAGGG TTATGATGGA TGGCAGATTC TGCACCCAAG TGCTCCTAAT
GGAGGTGGAG TCCTGGGGTC CTGTGATCTG GTGCCGGTCA GAGCAGTCAA GGAGGGGACG
CTGGGGCTGA CCCCAGCAGT GTCAGACCTT TTTGCTGCCA TAAATGCCTC ATGTGTGGTC
TGGAAGTGCT GTGAGGATGG GACACTGGAG TTGACTGACT CCAACACAAA GTATGTTGGC
AACAACATCA GCACCAAGGG TGTGGGCAGT GACCGCTGCG AGGACATCAC TCAGAACTAC
AAGTATCCTG AAGGGTCTCT TCAGGAAAAA GAGGTGCTGG AGAGAGTCGA GAAAGAGAAA
ATGGAACGTG AGAAAGACAA CGGCATCCGT CCTCCCAGTC TCGAGACTGC CAGTCCTCTG
TACCTGCTCT TGAAAGCACC CAGCTCCCTA CCCCTGAGAG GGGATGCCCA GATCTCAGTG
ACGCTGGTTA ATCACAGTGA GCAGGAGAAG GCAGTGCAGC TGGCAATTGG GGTCCAGGCT
GTACACTACA ACGGTGTCCT TGCTGCCAAG CTCTGGAGGA AGAAGCTGCA CCTCACGCTC
AGTGCCAACC TGGAAAAGAT AATAACCATC GGCCTGTTCT TCTCCAATTT TGAGCGAAAC
CCACCCGAGA ACACCTTCCT TAGACTCACC GCCATGGCAA CACACTCTGA ATCCAACCTT
AGCTGCTTTG CTCAGGAAGA CATTGCCATT TGTAGACCAC ACCTTGCCAT CAAGATGCCA
GAGAAAGCAG AGCAGTATCA ACCCCTCACA GCCTCAGTCA GCCTCCAGAA CTCCCTAGAT
GCCCCCATGG AGGACTGTGT GATCTCCATC CTGGGAAGGG GGCTCATTCA CAGAGAGAGG
AGCTACAGAT TCCGTTCAGT GTGGCCTGAA AACACCATGT GTGCCAAGTT CCAGTTCACG
CCAACACATG TGGGGCTCCA GAGACTCACT GTGGAAGTGG ACTGCAACAT GTTCCAGAAC
CTAACCAACT ATAAAAGCGT CACCGTGGTA GCCCCTGAAC TATCAGCTTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254282.1
CDS138..2108
Misc Feature(1)156..506
Misc Feature(2)813..1088
Misc Feature(3)1455..1772
Misc Feature(4)1794..2090
Translation

Target ORF information:

RefSeq Version XM_005254225.1
Organism Homo sapiens(Human)
Definition Homo sapiens erythrocyte membrane protein band 4.2 (EPB42), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254225.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGGACAGG CCCTGGGTAT CAAGAGCTGT GACTTTCAGG CAGCAAGAAA CAATGAGGAG 
CACCACACCA AGGCCCTCAG CTCCCGGCGC CTCTTTGTGA GGAGGGGGCA GCCCTTCACC
ATCATCCTGT ACTTCCGCGC TCCAGTCCGT GCATTTCTGC CTGCCCTGAA GAAGGTGGCC
CTCACTGCAC AAACTGGAGA GCAGCCTTCC AAGATCAACA GGACCCAAGC CACATTCCCA
ATTTCCAGTC TGGGGGACCG AAAGTGGTGG AGTGCAGTGG TGGAGGAGAG AGATGCCCAG
TCCTGGACCA TCTCTGTGAC CACACCTGCG GACGCTGTCA TTGGCCACTA CTCGCTTCTG
CTGCAGGTCT CAGGCAGGAA GCAACTCCTC TTGGGTCAGT TCACACTGCT TTTTAACCCC
TGGAATAGAG AGGATGCTGT GTTCCTGAAG AATGAGGCTC AGCGCATGGA GTACTTGTTG
AACCAGAATG GTCTCATCTA CCTGGGTACA GCTGACTGCA TCCAGGCAGA GTCCTGGGAC
TTTGGCCAGC TGCATTTTCT CAAGGAGCAG AGGGTCCTGC CCACCCCGCA GACCCAGGCC
ACCCAGGAAG GGGCCTTGCT GAACAAGCGC CGGGGCAGCG TGCCCATCCT GCGGCAGTGG
CTCACCGGCC GAGGCCGACC TGTGTATGAT GGCCAGGCCT GGGTGTTGGC TGCTGTTGCT
TGCACAGTGC TGCGATGCCT GGGAATCCCT GCCCGCGTGG TGACCACGTT TGCCTCAGCA
CAGGGCACCG GTGGGCGTCT TCTCATAGAT GAATACTATA ATGAGGAGGG ACTTCAGAAC
GGAGAAGGCC AGAGAGGCAG AATCTGGATC TTCCAGACTT CCACAGAGTG CTGGATGACG
CGGCCTGCCT TGCCCCAGGG TTATGATGGA TGGCAGATTC TGCACCCAAG TGCTCCTAAT
GGAGGTGGAG TCCTGGGGTC CTGTGATCTG GTGCCGGTCA GAGCAGTCAA GGAGGGGACG
CTGGGGCTGA CCCCAGCAGT GTCAGACCTT TTTGCTGCCA TAAATGCCTC ATGTGTGGTC
TGGAAGTGCT GTGAGGATGG GACACTGGAG TTGACTGACT CCAACACAAA GTATGTTGGC
AACAACATCA GCACCAAGGG TGTGGGCAGT GACCGCTGCG AGGACATCAC TCAGAACTAC
AAGTATCCTG AAGGGTCTCT TCAGGAAAAA GAGGTGCTGG AGAGAGTCGA GAAAGAGAAA
ATGGAACGTG AGAAAGACAA CGGCATCCGT CCTCCCAGTC TCGAGACTGC CAGTCCTCTG
TACCTGCTCT TGAAAGCACC CAGCTCCCTA CCCCTGAGAG GGGATGCCCA GATCTCAGTG
ACGCTGGTTA ATCACAGTGA GCAGGAGAAG GCAGTGCAGC TGGCAATTGG GGTCCAGGCT
GTACACTACA ACGGTGTCCT TGCTGCCAAG CTCTGGAGGA AGAAGCTGCA CCTCACGCTC
AGTGCCAACC TGGAAAAGAT AATAACCATC GGCCTGTTCT TCTCCAATTT TGAGCGAAAC
CCACCCGAGA ACACCTTCCT TAGACTCACC GCCATGGCAA CACACTCTGA ATCCAACCTT
AGCTGCTTTG CTCAGGAAGA CATTGCCATT TGTAGACCAC ACCTTGCCAT CAAGATGCCA
GAGAAAGCAG AGCAGTATCA ACCCCTCACA GCCTCAGTCA GCCTCCAGAA CTCCCTAGAT
GCCCCCATGG AGGACTGTGT GATCTCCATC CTGGGAAGGG GGCTCATTCA CAGAGAGAGG
AGCTACAGAT TCCGTTCAGT GTGGCCTGAA AACACCATGT GTGCCAAGTT CCAGTTCACG
CCAACACATG TGGGGCTCCA GAGACTCACT GTGGAAGTGG ACTGCAACAT GTTCCAGAAC
CTAACCAACT ATAAAAGCGT CACCGTGGTA GCCCCTGAAC TATCAGCTTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18437
Clone ID Related Accession (Same CDS sequence) XM_011521351.2 , XM_011521350.2 , NM_000119.2 , XM_011521349.2
Accession Version NM_000119.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2166bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-10-06
Organism Homo sapiens(Human)
Product erythrocyte membrane protein band 4.2 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DW451374.1, M60298.1, BC096093.4 and BU741892.1. This sequence is a reference standard in the RefSeqGene project. On Jan 31, 2008 this sequence version replaced NM_000119.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M60298.1, M30647.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2142680 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGGACAGG GTGAGCCAAG CCAGCGCTCG ACAGGGCTTG CTGGACTGTA TGCAGCCCCC 
GCAGCATCAC CTGTTTTCAT TAAAGGAAGT GGGATGGATG CCCTGGGTAT CAAGAGCTGT
GACTTTCAGG CAGCAAGAAA CAATGAGGAG CACCACACCA AGGCCCTCAG CTCCCGGCGC
CTCTTTGTGA GGAGGGGGCA GCCCTTCACC ATCATCCTGT ACTTCCGCGC TCCAGTCCGT
GCATTTCTGC CTGCCCTGAA GAAGGTGGCC CTCACTGCAC AAACTGGAGA GCAGCCTTCC
AAGATCAACA GGACCCAAGC CACATTCCCA ATTTCCAGTC TGGGGGACCG AAAGTGGTGG
AGTGCAGTGG TGGAGGAGAG AGATGCCCAG TCCTGGACCA TCTCTGTGAC CACACCTGCG
GACGCTGTCA TTGGCCACTA CTCGCTTCTG CTGCAGGTCT CAGGCAGGAA GCAACTCCTC
TTGGGTCAGT TCACACTGCT TTTTAACCCC TGGAATAGAG AGGATGCTGT GTTCCTGAAG
AATGAGGCTC AGCGCATGGA GTACTTGTTG AACCAGAATG GTCTCATCTA CCTGGGTACA
GCTGACTGCA TCCAGGCAGA GTCCTGGGAC TTTGGCCAGT TCGAGGGGGA TGTCATTGAC
CTCAGCCTGC GCTTGCTGAG CAAGGACAAG CAGGTAGAGA AGTGGAGCCA GCCGGTGCAC
GTGGCCCGTG TGTTGGGTGC CTTGCTGCAT TTTCTCAAGG AGCAGAGGGT CCTGCCCACC
CCGCAGACCC AGGCCACCCA GGAAGGGGCC TTGCTGAACA AGCGCCGGGG CAGCGTGCCC
ATCCTGCGGC AGTGGCTCAC CGGCCGAGGC CGACCTGTGT ATGATGGCCA GGCCTGGGTG
TTGGCTGCTG TTGCTTGCAC AGTGCTGCGA TGCCTGGGAA TCCCTGCCCG CGTGGTGACC
ACGTTTGCCT CAGCACAGGG CACCGGTGGG CGTCTTCTCA TAGATGAATA CTATAATGAG
GAGGGACTTC AGAACGGAGA AGGCCAGAGA GGCAGAATCT GGATCTTCCA GACTTCCACA
GAGTGCTGGA TGACGCGGCC TGCCTTGCCC CAGGGTTATG ATGGATGGCA GATTCTGCAC
CCAAGTGCTC CTAATGGAGG TGGAGTCCTG GGGTCCTGTG ATCTGGTGCC GGTCAGAGCA
GTCAAGGAGG GGACGCTGGG GCTGACCCCA GCAGTGTCAG ACCTTTTTGC TGCCATAAAT
GCCTCATGTG TGGTCTGGAA GTGCTGTGAG GATGGGACAC TGGAGTTGAC TGACTCCAAC
ACAAAGTATG TTGGCAACAA CATCAGCACC AAGGGTGTGG GCAGTGACCG CTGCGAGGAC
ATCACTCAGA ACTACAAGTA TCCTGAAGGG TCTCTTCAGG AAAAAGAGGT GCTGGAGAGA
GTCGAGAAAG AGAAAATGGA ACGTGAGAAA GACAACGGCA TCCGTCCTCC CAGTCTCGAG
ACTGCCAGTC CTCTGTACCT GCTCTTGAAA GCACCCAGCT CCCTACCCCT GAGAGGGGAT
GCCCAGATCT CAGTGACGCT GGTTAATCAC AGTGAGCAGG AGAAGGCAGT GCAGCTGGCA
ATTGGGGTCC AGGCTGTACA CTACAACGGT GTCCTTGCTG CCAAGCTCTG GAGGAAGAAG
CTGCACCTCA CGCTCAGTGC CAACCTGGAA AAGATAATAA CCATCGGCCT GTTCTTCTCC
AATTTTGAGC GAAACCCACC CGAGAACACC TTCCTTAGAC TCACCGCCAT GGCAACACAC
TCTGAATCCA ACCTTAGCTG CTTTGCTCAG GAAGACATTG CCATTTGTAG ACCACACCTT
GCCATCAAGA TGCCAGAGAA AGCAGAGCAG TATCAACCCC TCACAGCCTC AGTCAGCCTC
CAGAACTCCC TAGATGCCCC CATGGAGGAC TGTGTGATCT CCATCCTGGG AAGGGGGCTC
ATTCACAGAG AGAGGAGCTA CAGATTCCGT TCAGTGTGGC CTGAAAACAC CATGTGTGCC
AAGTTCCAGT TCACGCCAAC ACATGTGGGG CTCCAGAGAC TCACTGTGGA AGTGGACTGC
AACATGTTCC AGAACCTAAC CAACTATAAA AGCGTCACCG TGGTAGCCCC TGAACTATCA
GCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000110.2
CDS301..2466
Misc Feature(1)280..282
Misc Feature(2)409..759
Misc Feature(3)1171..1446
Misc Feature(4)1813..2130
Misc Feature(5)2152..2448
Exon (1)1..400
Exon (2)401..586
Exon (3)587..820
Exon (4)821..939
Exon (5)940..1044
Exon (6)1045..1222
Exon (7)1223..1361
Exon (8)1362..1465
Exon (9)1466..1708
Exon (10)1709..2008
Exon (11)2009..2169
Exon (12)2170..2303
Exon (13)2304..2541
Translation

Target ORF information:

RefSeq Version NM_000119.2
Organism Homo sapiens(Human)
Definition Homo sapiens erythrocyte membrane protein band 4.2 (EPB42), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000119.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGGACAGG GTGAGCCAAG CCAGCGCTCG ACAGGGCTTG CTGGACTGTA TGCAGCCCCC 
GCAGCATCAC CTGTTTTCAT TAAAGGAAGT GGGATGGATG CCCTGGGTAT CAAGAGCTGT
GACTTTCAGG CAGCAAGAAA CAATGAGGAG CACCACACCA AGGCCCTCAG CTCCCGGCGC
CTCTTTGTGA GGAGGGGGCA GCCCTTCACC ATCATCCTGT ACTTCCGCGC TCCAGTCCGT
GCATTTCTGC CTGCCCTGAA GAAGGTGGCC CTCACTGCAC AAACTGGAGA GCAGCCTTCC
AAGATCAACA GGACCCAAGC CACATTCCCA ATTTCCAGTC TGGGGGACCG AAAGTGGTGG
AGTGCAGTGG TGGAGGAGAG AGATGCCCAG TCCTGGACCA TCTCTGTGAC CACACCTGCG
GACGCTGTCA TTGGCCACTA CTCGCTTCTG CTGCAGGTCT CAGGCAGGAA GCAACTCCTC
TTGGGTCAGT TCACACTGCT TTTTAACCCC TGGAATAGAG AGGATGCTGT GTTCCTGAAG
AATGAGGCTC AGCGCATGGA GTACTTGTTG AACCAGAATG GTCTCATCTA CCTGGGTACA
GCTGACTGCA TCCAGGCAGA GTCCTGGGAC TTTGGCCAGT TCGAGGGGGA TGTCATTGAC
CTCAGCCTGC GCTTGCTGAG CAAGGACAAG CAGGTAGAGA AGTGGAGCCA GCCGGTGCAC
GTGGCCCGTG TGTTGGGTGC CTTGCTGCAT TTTCTCAAGG AGCAGAGGGT CCTGCCCACC
CCGCAGACCC AGGCCACCCA GGAAGGGGCC TTGCTGAACA AGCGCCGGGG CAGCGTGCCC
ATCCTGCGGC AGTGGCTCAC CGGCCGAGGC CGACCTGTGT ATGATGGCCA GGCCTGGGTG
TTGGCTGCTG TTGCTTGCAC AGTGCTGCGA TGCCTGGGAA TCCCTGCCCG CGTGGTGACC
ACGTTTGCCT CAGCACAGGG CACCGGTGGG CGTCTTCTCA TAGATGAATA CTATAATGAG
GAGGGACTTC AGAACGGAGA AGGCCAGAGA GGCAGAATCT GGATCTTCCA GACTTCCACA
GAGTGCTGGA TGACGCGGCC TGCCTTGCCC CAGGGTTATG ATGGATGGCA GATTCTGCAC
CCAAGTGCTC CTAATGGAGG TGGAGTCCTG GGGTCCTGTG ATCTGGTGCC GGTCAGAGCA
GTCAAGGAGG GGACGCTGGG GCTGACCCCA GCAGTGTCAG ACCTTTTTGC TGCCATAAAT
GCCTCATGTG TGGTCTGGAA GTGCTGTGAG GATGGGACAC TGGAGTTGAC TGACTCCAAC
ACAAAGTATG TTGGCAACAA CATCAGCACC AAGGGTGTGG GCAGTGACCG CTGCGAGGAC
ATCACTCAGA ACTACAAGTA TCCTGAAGGG TCTCTTCAGG AAAAAGAGGT GCTGGAGAGA
GTCGAGAAAG AGAAAATGGA ACGTGAGAAA GACAACGGCA TCCGTCCTCC CAGTCTCGAG
ACTGCCAGTC CTCTGTACCT GCTCTTGAAA GCACCCAGCT CCCTACCCCT GAGAGGGGAT
GCCCAGATCT CAGTGACGCT GGTTAATCAC AGTGAGCAGG AGAAGGCAGT GCAGCTGGCA
ATTGGGGTCC AGGCTGTACA CTACAACGGT GTCCTTGCTG CCAAGCTCTG GAGGAAGAAG
CTGCACCTCA CGCTCAGTGC CAACCTGGAA AAGATAATAA CCATCGGCCT GTTCTTCTCC
AATTTTGAGC GAAACCCACC CGAGAACACC TTCCTTAGAC TCACCGCCAT GGCAACACAC
TCTGAATCCA ACCTTAGCTG CTTTGCTCAG GAAGACATTG CCATTTGTAG ACCACACCTT
GCCATCAAGA TGCCAGAGAA AGCAGAGCAG TATCAACCCC TCACAGCCTC AGTCAGCCTC
CAGAACTCCC TAGATGCCCC CATGGAGGAC TGTGTGATCT CCATCCTGGG AAGGGGGCTC
ATTCACAGAG AGAGGAGCTA CAGATTCCGT TCAGTGTGGC CTGAAAACAC CATGTGTGCC
AAGTTCCAGT TCACGCCAAC ACATGTGGGG CTCCAGAGAC TCACTGTGGA AGTGGACTGC
AACATGTTCC AGAACCTAAC CAACTATAAA AGCGTCACCG TGGTAGCCCC TGAACTATCA
GCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18437
Clone ID Related Accession (Same CDS sequence) XM_011521351.2 , XM_011521350.2 , NM_000119.2 , XM_011521349.2
Accession Version XM_011521351.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2166bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product erythrocyte membrane protein band 4.2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521351.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGGACAGG GTGAGCCAAG CCAGCGCTCG ACAGGGCTTG CTGGACTGTA TGCAGCCCCC 
GCAGCATCAC CTGTTTTCAT TAAAGGAAGT GGGATGGATG CCCTGGGTAT CAAGAGCTGT
GACTTTCAGG CAGCAAGAAA CAATGAGGAG CACCACACCA AGGCCCTCAG CTCCCGGCGC
CTCTTTGTGA GGAGGGGGCA GCCCTTCACC ATCATCCTGT ACTTCCGCGC TCCAGTCCGT
GCATTTCTGC CTGCCCTGAA GAAGGTGGCC CTCACTGCAC AAACTGGAGA GCAGCCTTCC
AAGATCAACA GGACCCAAGC CACATTCCCA ATTTCCAGTC TGGGGGACCG AAAGTGGTGG
AGTGCAGTGG TGGAGGAGAG AGATGCCCAG TCCTGGACCA TCTCTGTGAC CACACCTGCG
GACGCTGTCA TTGGCCACTA CTCGCTTCTG CTGCAGGTCT CAGGCAGGAA GCAACTCCTC
TTGGGTCAGT TCACACTGCT TTTTAACCCC TGGAATAGAG AGGATGCTGT GTTCCTGAAG
AATGAGGCTC AGCGCATGGA GTACTTGTTG AACCAGAATG GTCTCATCTA CCTGGGTACA
GCTGACTGCA TCCAGGCAGA GTCCTGGGAC TTTGGCCAGT TCGAGGGGGA TGTCATTGAC
CTCAGCCTGC GCTTGCTGAG CAAGGACAAG CAGGTAGAGA AGTGGAGCCA GCCGGTGCAC
GTGGCCCGTG TGTTGGGTGC CTTGCTGCAT TTTCTCAAGG AGCAGAGGGT CCTGCCCACC
CCGCAGACCC AGGCCACCCA GGAAGGGGCC TTGCTGAACA AGCGCCGGGG CAGCGTGCCC
ATCCTGCGGC AGTGGCTCAC CGGCCGAGGC CGACCTGTGT ATGATGGCCA GGCCTGGGTG
TTGGCTGCTG TTGCTTGCAC AGTGCTGCGA TGCCTGGGAA TCCCTGCCCG CGTGGTGACC
ACGTTTGCCT CAGCACAGGG CACCGGTGGG CGTCTTCTCA TAGATGAATA CTATAATGAG
GAGGGACTTC AGAACGGAGA AGGCCAGAGA GGCAGAATCT GGATCTTCCA GACTTCCACA
GAGTGCTGGA TGACGCGGCC TGCCTTGCCC CAGGGTTATG ATGGATGGCA GATTCTGCAC
CCAAGTGCTC CTAATGGAGG TGGAGTCCTG GGGTCCTGTG ATCTGGTGCC GGTCAGAGCA
GTCAAGGAGG GGACGCTGGG GCTGACCCCA GCAGTGTCAG ACCTTTTTGC TGCCATAAAT
GCCTCATGTG TGGTCTGGAA GTGCTGTGAG GATGGGACAC TGGAGTTGAC TGACTCCAAC
ACAAAGTATG TTGGCAACAA CATCAGCACC AAGGGTGTGG GCAGTGACCG CTGCGAGGAC
ATCACTCAGA ACTACAAGTA TCCTGAAGGG TCTCTTCAGG AAAAAGAGGT GCTGGAGAGA
GTCGAGAAAG AGAAAATGGA ACGTGAGAAA GACAACGGCA TCCGTCCTCC CAGTCTCGAG
ACTGCCAGTC CTCTGTACCT GCTCTTGAAA GCACCCAGCT CCCTACCCCT GAGAGGGGAT
GCCCAGATCT CAGTGACGCT GGTTAATCAC AGTGAGCAGG AGAAGGCAGT GCAGCTGGCA
ATTGGGGTCC AGGCTGTACA CTACAACGGT GTCCTTGCTG CCAAGCTCTG GAGGAAGAAG
CTGCACCTCA CGCTCAGTGC CAACCTGGAA AAGATAATAA CCATCGGCCT GTTCTTCTCC
AATTTTGAGC GAAACCCACC CGAGAACACC TTCCTTAGAC TCACCGCCAT GGCAACACAC
TCTGAATCCA ACCTTAGCTG CTTTGCTCAG GAAGACATTG CCATTTGTAG ACCACACCTT
GCCATCAAGA TGCCAGAGAA AGCAGAGCAG TATCAACCCC TCACAGCCTC AGTCAGCCTC
CAGAACTCCC TAGATGCCCC CATGGAGGAC TGTGTGATCT CCATCCTGGG AAGGGGGCTC
ATTCACAGAG AGAGGAGCTA CAGATTCCGT TCAGTGTGGC CTGAAAACAC CATGTGTGCC
AAGTTCCAGT TCACGCCAAC ACATGTGGGG CTCCAGAGAC TCACTGTGGA AGTGGACTGC
AACATGTTCC AGAACCTAAC CAACTATAAA AGCGTCACCG TGGTAGCCCC TGAACTATCA
GCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011519653.1
CDS623..2788
Misc Feature(1)731..1081
Misc Feature(2)1493..1768
Misc Feature(3)2135..2452
Misc Feature(4)2474..2770
Translation

Target ORF information:

RefSeq Version XM_011521351.2
Organism Homo sapiens(Human)
Definition Homo sapiens erythrocyte membrane protein band 4.2 (EPB42), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011521351.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGGACAGG GTGAGCCAAG CCAGCGCTCG ACAGGGCTTG CTGGACTGTA TGCAGCCCCC 
GCAGCATCAC CTGTTTTCAT TAAAGGAAGT GGGATGGATG CCCTGGGTAT CAAGAGCTGT
GACTTTCAGG CAGCAAGAAA CAATGAGGAG CACCACACCA AGGCCCTCAG CTCCCGGCGC
CTCTTTGTGA GGAGGGGGCA GCCCTTCACC ATCATCCTGT ACTTCCGCGC TCCAGTCCGT
GCATTTCTGC CTGCCCTGAA GAAGGTGGCC CTCACTGCAC AAACTGGAGA GCAGCCTTCC
AAGATCAACA GGACCCAAGC CACATTCCCA ATTTCCAGTC TGGGGGACCG AAAGTGGTGG
AGTGCAGTGG TGGAGGAGAG AGATGCCCAG TCCTGGACCA TCTCTGTGAC CACACCTGCG
GACGCTGTCA TTGGCCACTA CTCGCTTCTG CTGCAGGTCT CAGGCAGGAA GCAACTCCTC
TTGGGTCAGT TCACACTGCT TTTTAACCCC TGGAATAGAG AGGATGCTGT GTTCCTGAAG
AATGAGGCTC AGCGCATGGA GTACTTGTTG AACCAGAATG GTCTCATCTA CCTGGGTACA
GCTGACTGCA TCCAGGCAGA GTCCTGGGAC TTTGGCCAGT TCGAGGGGGA TGTCATTGAC
CTCAGCCTGC GCTTGCTGAG CAAGGACAAG CAGGTAGAGA AGTGGAGCCA GCCGGTGCAC
GTGGCCCGTG TGTTGGGTGC CTTGCTGCAT TTTCTCAAGG AGCAGAGGGT CCTGCCCACC
CCGCAGACCC AGGCCACCCA GGAAGGGGCC TTGCTGAACA AGCGCCGGGG CAGCGTGCCC
ATCCTGCGGC AGTGGCTCAC CGGCCGAGGC CGACCTGTGT ATGATGGCCA GGCCTGGGTG
TTGGCTGCTG TTGCTTGCAC AGTGCTGCGA TGCCTGGGAA TCCCTGCCCG CGTGGTGACC
ACGTTTGCCT CAGCACAGGG CACCGGTGGG CGTCTTCTCA TAGATGAATA CTATAATGAG
GAGGGACTTC AGAACGGAGA AGGCCAGAGA GGCAGAATCT GGATCTTCCA GACTTCCACA
GAGTGCTGGA TGACGCGGCC TGCCTTGCCC CAGGGTTATG ATGGATGGCA GATTCTGCAC
CCAAGTGCTC CTAATGGAGG TGGAGTCCTG GGGTCCTGTG ATCTGGTGCC GGTCAGAGCA
GTCAAGGAGG GGACGCTGGG GCTGACCCCA GCAGTGTCAG ACCTTTTTGC TGCCATAAAT
GCCTCATGTG TGGTCTGGAA GTGCTGTGAG GATGGGACAC TGGAGTTGAC TGACTCCAAC
ACAAAGTATG TTGGCAACAA CATCAGCACC AAGGGTGTGG GCAGTGACCG CTGCGAGGAC
ATCACTCAGA ACTACAAGTA TCCTGAAGGG TCTCTTCAGG AAAAAGAGGT GCTGGAGAGA
GTCGAGAAAG AGAAAATGGA ACGTGAGAAA GACAACGGCA TCCGTCCTCC CAGTCTCGAG
ACTGCCAGTC CTCTGTACCT GCTCTTGAAA GCACCCAGCT CCCTACCCCT GAGAGGGGAT
GCCCAGATCT CAGTGACGCT GGTTAATCAC AGTGAGCAGG AGAAGGCAGT GCAGCTGGCA
ATTGGGGTCC AGGCTGTACA CTACAACGGT GTCCTTGCTG CCAAGCTCTG GAGGAAGAAG
CTGCACCTCA CGCTCAGTGC CAACCTGGAA AAGATAATAA CCATCGGCCT GTTCTTCTCC
AATTTTGAGC GAAACCCACC CGAGAACACC TTCCTTAGAC TCACCGCCAT GGCAACACAC
TCTGAATCCA ACCTTAGCTG CTTTGCTCAG GAAGACATTG CCATTTGTAG ACCACACCTT
GCCATCAAGA TGCCAGAGAA AGCAGAGCAG TATCAACCCC TCACAGCCTC AGTCAGCCTC
CAGAACTCCC TAGATGCCCC CATGGAGGAC TGTGTGATCT CCATCCTGGG AAGGGGGCTC
ATTCACAGAG AGAGGAGCTA CAGATTCCGT TCAGTGTGGC CTGAAAACAC CATGTGTGCC
AAGTTCCAGT TCACGCCAAC ACATGTGGGG CTCCAGAGAC TCACTGTGGA AGTGGACTGC
AACATGTTCC AGAACCTAAC CAACTATAAA AGCGTCACCG TGGTAGCCCC TGAACTATCA
GCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18437
Clone ID Related Accession (Same CDS sequence) XM_011521351.2 , XM_011521350.2 , NM_000119.2 , XM_011521349.2
Accession Version XM_011521350.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2166bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product erythrocyte membrane protein band 4.2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521350.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGGACAGG GTGAGCCAAG CCAGCGCTCG ACAGGGCTTG CTGGACTGTA TGCAGCCCCC 
GCAGCATCAC CTGTTTTCAT TAAAGGAAGT GGGATGGATG CCCTGGGTAT CAAGAGCTGT
GACTTTCAGG CAGCAAGAAA CAATGAGGAG CACCACACCA AGGCCCTCAG CTCCCGGCGC
CTCTTTGTGA GGAGGGGGCA GCCCTTCACC ATCATCCTGT ACTTCCGCGC TCCAGTCCGT
GCATTTCTGC CTGCCCTGAA GAAGGTGGCC CTCACTGCAC AAACTGGAGA GCAGCCTTCC
AAGATCAACA GGACCCAAGC CACATTCCCA ATTTCCAGTC TGGGGGACCG AAAGTGGTGG
AGTGCAGTGG TGGAGGAGAG AGATGCCCAG TCCTGGACCA TCTCTGTGAC CACACCTGCG
GACGCTGTCA TTGGCCACTA CTCGCTTCTG CTGCAGGTCT CAGGCAGGAA GCAACTCCTC
TTGGGTCAGT TCACACTGCT TTTTAACCCC TGGAATAGAG AGGATGCTGT GTTCCTGAAG
AATGAGGCTC AGCGCATGGA GTACTTGTTG AACCAGAATG GTCTCATCTA CCTGGGTACA
GCTGACTGCA TCCAGGCAGA GTCCTGGGAC TTTGGCCAGT TCGAGGGGGA TGTCATTGAC
CTCAGCCTGC GCTTGCTGAG CAAGGACAAG CAGGTAGAGA AGTGGAGCCA GCCGGTGCAC
GTGGCCCGTG TGTTGGGTGC CTTGCTGCAT TTTCTCAAGG AGCAGAGGGT CCTGCCCACC
CCGCAGACCC AGGCCACCCA GGAAGGGGCC TTGCTGAACA AGCGCCGGGG CAGCGTGCCC
ATCCTGCGGC AGTGGCTCAC CGGCCGAGGC CGACCTGTGT ATGATGGCCA GGCCTGGGTG
TTGGCTGCTG TTGCTTGCAC AGTGCTGCGA TGCCTGGGAA TCCCTGCCCG CGTGGTGACC
ACGTTTGCCT CAGCACAGGG CACCGGTGGG CGTCTTCTCA TAGATGAATA CTATAATGAG
GAGGGACTTC AGAACGGAGA AGGCCAGAGA GGCAGAATCT GGATCTTCCA GACTTCCACA
GAGTGCTGGA TGACGCGGCC TGCCTTGCCC CAGGGTTATG ATGGATGGCA GATTCTGCAC
CCAAGTGCTC CTAATGGAGG TGGAGTCCTG GGGTCCTGTG ATCTGGTGCC GGTCAGAGCA
GTCAAGGAGG GGACGCTGGG GCTGACCCCA GCAGTGTCAG ACCTTTTTGC TGCCATAAAT
GCCTCATGTG TGGTCTGGAA GTGCTGTGAG GATGGGACAC TGGAGTTGAC TGACTCCAAC
ACAAAGTATG TTGGCAACAA CATCAGCACC AAGGGTGTGG GCAGTGACCG CTGCGAGGAC
ATCACTCAGA ACTACAAGTA TCCTGAAGGG TCTCTTCAGG AAAAAGAGGT GCTGGAGAGA
GTCGAGAAAG AGAAAATGGA ACGTGAGAAA GACAACGGCA TCCGTCCTCC CAGTCTCGAG
ACTGCCAGTC CTCTGTACCT GCTCTTGAAA GCACCCAGCT CCCTACCCCT GAGAGGGGAT
GCCCAGATCT CAGTGACGCT GGTTAATCAC AGTGAGCAGG AGAAGGCAGT GCAGCTGGCA
ATTGGGGTCC AGGCTGTACA CTACAACGGT GTCCTTGCTG CCAAGCTCTG GAGGAAGAAG
CTGCACCTCA CGCTCAGTGC CAACCTGGAA AAGATAATAA CCATCGGCCT GTTCTTCTCC
AATTTTGAGC GAAACCCACC CGAGAACACC TTCCTTAGAC TCACCGCCAT GGCAACACAC
TCTGAATCCA ACCTTAGCTG CTTTGCTCAG GAAGACATTG CCATTTGTAG ACCACACCTT
GCCATCAAGA TGCCAGAGAA AGCAGAGCAG TATCAACCCC TCACAGCCTC AGTCAGCCTC
CAGAACTCCC TAGATGCCCC CATGGAGGAC TGTGTGATCT CCATCCTGGG AAGGGGGCTC
ATTCACAGAG AGAGGAGCTA CAGATTCCGT TCAGTGTGGC CTGAAAACAC CATGTGTGCC
AAGTTCCAGT TCACGCCAAC ACATGTGGGG CTCCAGAGAC TCACTGTGGA AGTGGACTGC
AACATGTTCC AGAACCTAAC CAACTATAAA AGCGTCACCG TGGTAGCCCC TGAACTATCA
GCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011519652.1
CDS761..2926
Misc Feature(1)869..1219
Misc Feature(2)1631..1906
Misc Feature(3)2273..2590
Misc Feature(4)2612..2908
Translation

Target ORF information:

RefSeq Version XM_011521350.2
Organism Homo sapiens(Human)
Definition Homo sapiens erythrocyte membrane protein band 4.2 (EPB42), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011521350.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGGACAGG GTGAGCCAAG CCAGCGCTCG ACAGGGCTTG CTGGACTGTA TGCAGCCCCC 
GCAGCATCAC CTGTTTTCAT TAAAGGAAGT GGGATGGATG CCCTGGGTAT CAAGAGCTGT
GACTTTCAGG CAGCAAGAAA CAATGAGGAG CACCACACCA AGGCCCTCAG CTCCCGGCGC
CTCTTTGTGA GGAGGGGGCA GCCCTTCACC ATCATCCTGT ACTTCCGCGC TCCAGTCCGT
GCATTTCTGC CTGCCCTGAA GAAGGTGGCC CTCACTGCAC AAACTGGAGA GCAGCCTTCC
AAGATCAACA GGACCCAAGC CACATTCCCA ATTTCCAGTC TGGGGGACCG AAAGTGGTGG
AGTGCAGTGG TGGAGGAGAG AGATGCCCAG TCCTGGACCA TCTCTGTGAC CACACCTGCG
GACGCTGTCA TTGGCCACTA CTCGCTTCTG CTGCAGGTCT CAGGCAGGAA GCAACTCCTC
TTGGGTCAGT TCACACTGCT TTTTAACCCC TGGAATAGAG AGGATGCTGT GTTCCTGAAG
AATGAGGCTC AGCGCATGGA GTACTTGTTG AACCAGAATG GTCTCATCTA CCTGGGTACA
GCTGACTGCA TCCAGGCAGA GTCCTGGGAC TTTGGCCAGT TCGAGGGGGA TGTCATTGAC
CTCAGCCTGC GCTTGCTGAG CAAGGACAAG CAGGTAGAGA AGTGGAGCCA GCCGGTGCAC
GTGGCCCGTG TGTTGGGTGC CTTGCTGCAT TTTCTCAAGG AGCAGAGGGT CCTGCCCACC
CCGCAGACCC AGGCCACCCA GGAAGGGGCC TTGCTGAACA AGCGCCGGGG CAGCGTGCCC
ATCCTGCGGC AGTGGCTCAC CGGCCGAGGC CGACCTGTGT ATGATGGCCA GGCCTGGGTG
TTGGCTGCTG TTGCTTGCAC AGTGCTGCGA TGCCTGGGAA TCCCTGCCCG CGTGGTGACC
ACGTTTGCCT CAGCACAGGG CACCGGTGGG CGTCTTCTCA TAGATGAATA CTATAATGAG
GAGGGACTTC AGAACGGAGA AGGCCAGAGA GGCAGAATCT GGATCTTCCA GACTTCCACA
GAGTGCTGGA TGACGCGGCC TGCCTTGCCC CAGGGTTATG ATGGATGGCA GATTCTGCAC
CCAAGTGCTC CTAATGGAGG TGGAGTCCTG GGGTCCTGTG ATCTGGTGCC GGTCAGAGCA
GTCAAGGAGG GGACGCTGGG GCTGACCCCA GCAGTGTCAG ACCTTTTTGC TGCCATAAAT
GCCTCATGTG TGGTCTGGAA GTGCTGTGAG GATGGGACAC TGGAGTTGAC TGACTCCAAC
ACAAAGTATG TTGGCAACAA CATCAGCACC AAGGGTGTGG GCAGTGACCG CTGCGAGGAC
ATCACTCAGA ACTACAAGTA TCCTGAAGGG TCTCTTCAGG AAAAAGAGGT GCTGGAGAGA
GTCGAGAAAG AGAAAATGGA ACGTGAGAAA GACAACGGCA TCCGTCCTCC CAGTCTCGAG
ACTGCCAGTC CTCTGTACCT GCTCTTGAAA GCACCCAGCT CCCTACCCCT GAGAGGGGAT
GCCCAGATCT CAGTGACGCT GGTTAATCAC AGTGAGCAGG AGAAGGCAGT GCAGCTGGCA
ATTGGGGTCC AGGCTGTACA CTACAACGGT GTCCTTGCTG CCAAGCTCTG GAGGAAGAAG
CTGCACCTCA CGCTCAGTGC CAACCTGGAA AAGATAATAA CCATCGGCCT GTTCTTCTCC
AATTTTGAGC GAAACCCACC CGAGAACACC TTCCTTAGAC TCACCGCCAT GGCAACACAC
TCTGAATCCA ACCTTAGCTG CTTTGCTCAG GAAGACATTG CCATTTGTAG ACCACACCTT
GCCATCAAGA TGCCAGAGAA AGCAGAGCAG TATCAACCCC TCACAGCCTC AGTCAGCCTC
CAGAACTCCC TAGATGCCCC CATGGAGGAC TGTGTGATCT CCATCCTGGG AAGGGGGCTC
ATTCACAGAG AGAGGAGCTA CAGATTCCGT TCAGTGTGGC CTGAAAACAC CATGTGTGCC
AAGTTCCAGT TCACGCCAAC ACATGTGGGG CTCCAGAGAC TCACTGTGGA AGTGGACTGC
AACATGTTCC AGAACCTAAC CAACTATAAA AGCGTCACCG TGGTAGCCCC TGAACTATCA
GCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18437
Clone ID Related Accession (Same CDS sequence) XM_011521351.2 , XM_011521350.2 , NM_000119.2 , XM_011521349.2
Accession Version XM_011521349.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2166bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product erythrocyte membrane protein band 4.2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521349.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGGACAGG GTGAGCCAAG CCAGCGCTCG ACAGGGCTTG CTGGACTGTA TGCAGCCCCC 
GCAGCATCAC CTGTTTTCAT TAAAGGAAGT GGGATGGATG CCCTGGGTAT CAAGAGCTGT
GACTTTCAGG CAGCAAGAAA CAATGAGGAG CACCACACCA AGGCCCTCAG CTCCCGGCGC
CTCTTTGTGA GGAGGGGGCA GCCCTTCACC ATCATCCTGT ACTTCCGCGC TCCAGTCCGT
GCATTTCTGC CTGCCCTGAA GAAGGTGGCC CTCACTGCAC AAACTGGAGA GCAGCCTTCC
AAGATCAACA GGACCCAAGC CACATTCCCA ATTTCCAGTC TGGGGGACCG AAAGTGGTGG
AGTGCAGTGG TGGAGGAGAG AGATGCCCAG TCCTGGACCA TCTCTGTGAC CACACCTGCG
GACGCTGTCA TTGGCCACTA CTCGCTTCTG CTGCAGGTCT CAGGCAGGAA GCAACTCCTC
TTGGGTCAGT TCACACTGCT TTTTAACCCC TGGAATAGAG AGGATGCTGT GTTCCTGAAG
AATGAGGCTC AGCGCATGGA GTACTTGTTG AACCAGAATG GTCTCATCTA CCTGGGTACA
GCTGACTGCA TCCAGGCAGA GTCCTGGGAC TTTGGCCAGT TCGAGGGGGA TGTCATTGAC
CTCAGCCTGC GCTTGCTGAG CAAGGACAAG CAGGTAGAGA AGTGGAGCCA GCCGGTGCAC
GTGGCCCGTG TGTTGGGTGC CTTGCTGCAT TTTCTCAAGG AGCAGAGGGT CCTGCCCACC
CCGCAGACCC AGGCCACCCA GGAAGGGGCC TTGCTGAACA AGCGCCGGGG CAGCGTGCCC
ATCCTGCGGC AGTGGCTCAC CGGCCGAGGC CGACCTGTGT ATGATGGCCA GGCCTGGGTG
TTGGCTGCTG TTGCTTGCAC AGTGCTGCGA TGCCTGGGAA TCCCTGCCCG CGTGGTGACC
ACGTTTGCCT CAGCACAGGG CACCGGTGGG CGTCTTCTCA TAGATGAATA CTATAATGAG
GAGGGACTTC AGAACGGAGA AGGCCAGAGA GGCAGAATCT GGATCTTCCA GACTTCCACA
GAGTGCTGGA TGACGCGGCC TGCCTTGCCC CAGGGTTATG ATGGATGGCA GATTCTGCAC
CCAAGTGCTC CTAATGGAGG TGGAGTCCTG GGGTCCTGTG ATCTGGTGCC GGTCAGAGCA
GTCAAGGAGG GGACGCTGGG GCTGACCCCA GCAGTGTCAG ACCTTTTTGC TGCCATAAAT
GCCTCATGTG TGGTCTGGAA GTGCTGTGAG GATGGGACAC TGGAGTTGAC TGACTCCAAC
ACAAAGTATG TTGGCAACAA CATCAGCACC AAGGGTGTGG GCAGTGACCG CTGCGAGGAC
ATCACTCAGA ACTACAAGTA TCCTGAAGGG TCTCTTCAGG AAAAAGAGGT GCTGGAGAGA
GTCGAGAAAG AGAAAATGGA ACGTGAGAAA GACAACGGCA TCCGTCCTCC CAGTCTCGAG
ACTGCCAGTC CTCTGTACCT GCTCTTGAAA GCACCCAGCT CCCTACCCCT GAGAGGGGAT
GCCCAGATCT CAGTGACGCT GGTTAATCAC AGTGAGCAGG AGAAGGCAGT GCAGCTGGCA
ATTGGGGTCC AGGCTGTACA CTACAACGGT GTCCTTGCTG CCAAGCTCTG GAGGAAGAAG
CTGCACCTCA CGCTCAGTGC CAACCTGGAA AAGATAATAA CCATCGGCCT GTTCTTCTCC
AATTTTGAGC GAAACCCACC CGAGAACACC TTCCTTAGAC TCACCGCCAT GGCAACACAC
TCTGAATCCA ACCTTAGCTG CTTTGCTCAG GAAGACATTG CCATTTGTAG ACCACACCTT
GCCATCAAGA TGCCAGAGAA AGCAGAGCAG TATCAACCCC TCACAGCCTC AGTCAGCCTC
CAGAACTCCC TAGATGCCCC CATGGAGGAC TGTGTGATCT CCATCCTGGG AAGGGGGCTC
ATTCACAGAG AGAGGAGCTA CAGATTCCGT TCAGTGTGGC CTGAAAACAC CATGTGTGCC
AAGTTCCAGT TCACGCCAAC ACATGTGGGG CTCCAGAGAC TCACTGTGGA AGTGGACTGC
AACATGTTCC AGAACCTAAC CAACTATAAA AGCGTCACCG TGGTAGCCCC TGAACTATCA
GCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011519651.1
CDS1029..3194
Misc Feature(1)1137..1487
Misc Feature(2)1899..2174
Misc Feature(3)2541..2858
Misc Feature(4)2880..3176
Translation

Target ORF information:

RefSeq Version XM_011521349.2
Organism Homo sapiens(Human)
Definition Homo sapiens erythrocyte membrane protein band 4.2 (EPB42), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011521349.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGGACAGG GTGAGCCAAG CCAGCGCTCG ACAGGGCTTG CTGGACTGTA TGCAGCCCCC 
GCAGCATCAC CTGTTTTCAT TAAAGGAAGT GGGATGGATG CCCTGGGTAT CAAGAGCTGT
GACTTTCAGG CAGCAAGAAA CAATGAGGAG CACCACACCA AGGCCCTCAG CTCCCGGCGC
CTCTTTGTGA GGAGGGGGCA GCCCTTCACC ATCATCCTGT ACTTCCGCGC TCCAGTCCGT
GCATTTCTGC CTGCCCTGAA GAAGGTGGCC CTCACTGCAC AAACTGGAGA GCAGCCTTCC
AAGATCAACA GGACCCAAGC CACATTCCCA ATTTCCAGTC TGGGGGACCG AAAGTGGTGG
AGTGCAGTGG TGGAGGAGAG AGATGCCCAG TCCTGGACCA TCTCTGTGAC CACACCTGCG
GACGCTGTCA TTGGCCACTA CTCGCTTCTG CTGCAGGTCT CAGGCAGGAA GCAACTCCTC
TTGGGTCAGT TCACACTGCT TTTTAACCCC TGGAATAGAG AGGATGCTGT GTTCCTGAAG
AATGAGGCTC AGCGCATGGA GTACTTGTTG AACCAGAATG GTCTCATCTA CCTGGGTACA
GCTGACTGCA TCCAGGCAGA GTCCTGGGAC TTTGGCCAGT TCGAGGGGGA TGTCATTGAC
CTCAGCCTGC GCTTGCTGAG CAAGGACAAG CAGGTAGAGA AGTGGAGCCA GCCGGTGCAC
GTGGCCCGTG TGTTGGGTGC CTTGCTGCAT TTTCTCAAGG AGCAGAGGGT CCTGCCCACC
CCGCAGACCC AGGCCACCCA GGAAGGGGCC TTGCTGAACA AGCGCCGGGG CAGCGTGCCC
ATCCTGCGGC AGTGGCTCAC CGGCCGAGGC CGACCTGTGT ATGATGGCCA GGCCTGGGTG
TTGGCTGCTG TTGCTTGCAC AGTGCTGCGA TGCCTGGGAA TCCCTGCCCG CGTGGTGACC
ACGTTTGCCT CAGCACAGGG CACCGGTGGG CGTCTTCTCA TAGATGAATA CTATAATGAG
GAGGGACTTC AGAACGGAGA AGGCCAGAGA GGCAGAATCT GGATCTTCCA GACTTCCACA
GAGTGCTGGA TGACGCGGCC TGCCTTGCCC CAGGGTTATG ATGGATGGCA GATTCTGCAC
CCAAGTGCTC CTAATGGAGG TGGAGTCCTG GGGTCCTGTG ATCTGGTGCC GGTCAGAGCA
GTCAAGGAGG GGACGCTGGG GCTGACCCCA GCAGTGTCAG ACCTTTTTGC TGCCATAAAT
GCCTCATGTG TGGTCTGGAA GTGCTGTGAG GATGGGACAC TGGAGTTGAC TGACTCCAAC
ACAAAGTATG TTGGCAACAA CATCAGCACC AAGGGTGTGG GCAGTGACCG CTGCGAGGAC
ATCACTCAGA ACTACAAGTA TCCTGAAGGG TCTCTTCAGG AAAAAGAGGT GCTGGAGAGA
GTCGAGAAAG AGAAAATGGA ACGTGAGAAA GACAACGGCA TCCGTCCTCC CAGTCTCGAG
ACTGCCAGTC CTCTGTACCT GCTCTTGAAA GCACCCAGCT CCCTACCCCT GAGAGGGGAT
GCCCAGATCT CAGTGACGCT GGTTAATCAC AGTGAGCAGG AGAAGGCAGT GCAGCTGGCA
ATTGGGGTCC AGGCTGTACA CTACAACGGT GTCCTTGCTG CCAAGCTCTG GAGGAAGAAG
CTGCACCTCA CGCTCAGTGC CAACCTGGAA AAGATAATAA CCATCGGCCT GTTCTTCTCC
AATTTTGAGC GAAACCCACC CGAGAACACC TTCCTTAGAC TCACCGCCAT GGCAACACAC
TCTGAATCCA ACCTTAGCTG CTTTGCTCAG GAAGACATTG CCATTTGTAG ACCACACCTT
GCCATCAAGA TGCCAGAGAA AGCAGAGCAG TATCAACCCC TCACAGCCTC AGTCAGCCTC
CAGAACTCCC TAGATGCCCC CATGGAGGAC TGTGTGATCT CCATCCTGGG AAGGGGGCTC
ATTCACAGAG AGAGGAGCTA CAGATTCCGT TCAGTGTGGC CTGAAAACAC CATGTGTGCC
AAGTTCCAGT TCACGCCAAC ACATGTGGGG CTCCAGAGAC TCACTGTGGA AGTGGACTGC
AACATGTTCC AGAACCTAAC CAACTATAAA AGCGTCACCG TGGTAGCCCC TGAACTATCA
GCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57538
Clone ID Related Accession (Same CDS sequence) XM_011521352.2
Accession Version XM_011521352.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2130bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product erythrocyte membrane protein band 4.2 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521352.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGGGACAGG GTGAGCCAAG CCAGCGCTCG ACAGGGCTTG CTGGACTGTA TGCAGCCCCC 
GCAGCATCAC CTGTTTTCAT TAAAGGAAGT GGGATGGATG CCCTGGGTAT CAAGAGCTGT
GACTTTCAGG CAGCAAGAAA CAATGAGGAG CACCACACCA AGGCCCTCAG CTCCCGGCGC
CTCTTTGTGA GGAGGGGGCA GCCCTTCACC ATCATCCTGT ACTTCCGCGC TCCAGTCCGT
GCATTTCTGC CTGCCCTGAA GAAGGTGGCC CTCACTGCAC AAACTGGAGA GCAGCCTTCC
AAGATCAACA GGACCCAAGC CACATTCCCA ATTTCCAGTC TGGGGGACCG AAAGTGGTGG
AGTGCAGTGG TGGAGGAGAG AGATGCCCAG TCCTGGACCA TCTCTGTGAC CACACCTGCG
GACGCTGTCA TTGGCCACTA CTCGCTTCTG CTGCAGGTCT CAGGCAGGAA GCAACTCCTC
TTGGAGGATG CTGTGTTCCT GAAGAATGAG GCTCAGCGCA TGGAGTACTT GTTGAACCAG
AATGGTCTCA TCTACCTGGG TACAGCTGAC TGCATCCAGG CAGAGTCCTG GGACTTTGGC
CAGTTCGAGG GGGATGTCAT TGACCTCAGC CTGCGCTTGC TGAGCAAGGA CAAGCAGGTA
GAGAAGTGGA GCCAGCCGGT GCACGTGGCC CGTGTGTTGG GTGCCTTGCT GCATTTTCTC
AAGGAGCAGA GGGTCCTGCC CACCCCGCAG ACCCAGGCCA CCCAGGAAGG GGCCTTGCTG
AACAAGCGCC GGGGCAGCGT GCCCATCCTG CGGCAGTGGC TCACCGGCCG AGGCCGACCT
GTGTATGATG GCCAGGCCTG GGTGTTGGCT GCTGTTGCTT GCACAGTGCT GCGATGCCTG
GGAATCCCTG CCCGCGTGGT GACCACGTTT GCCTCAGCAC AGGGCACCGG TGGGCGTCTT
CTCATAGATG AATACTATAA TGAGGAGGGA CTTCAGAACG GAGAAGGCCA GAGAGGCAGA
ATCTGGATCT TCCAGACTTC CACAGAGTGC TGGATGACGC GGCCTGCCTT GCCCCAGGGT
TATGATGGAT GGCAGATTCT GCACCCAAGT GCTCCTAATG GAGGTGGAGT CCTGGGGTCC
TGTGATCTGG TGCCGGTCAG AGCAGTCAAG GAGGGGACGC TGGGGCTGAC CCCAGCAGTG
TCAGACCTTT TTGCTGCCAT AAATGCCTCA TGTGTGGTCT GGAAGTGCTG TGAGGATGGG
ACACTGGAGT TGACTGACTC CAACACAAAG TATGTTGGCA ACAACATCAG CACCAAGGGT
GTGGGCAGTG ACCGCTGCGA GGACATCACT CAGAACTACA AGTATCCTGA AGGGTCTCTT
CAGGAAAAAG AGGTGCTGGA GAGAGTCGAG AAAGAGAAAA TGGAACGTGA GAAAGACAAC
GGCATCCGTC CTCCCAGTCT CGAGACTGCC AGTCCTCTGT ACCTGCTCTT GAAAGCACCC
AGCTCCCTAC CCCTGAGAGG GGATGCCCAG ATCTCAGTGA CGCTGGTTAA TCACAGTGAG
CAGGAGAAGG CAGTGCAGCT GGCAATTGGG GTCCAGGCTG TACACTACAA CGGTGTCCTT
GCTGCCAAGC TCTGGAGGAA GAAGCTGCAC CTCACGCTCA GTGCCAACCT GGAAAAGATA
ATAACCATCG GCCTGTTCTT CTCCAATTTT GAGCGAAACC CACCCGAGAA CACCTTCCTT
AGACTCACCG CCATGGCAAC ACACTCTGAA TCCAACCTTA GCTGCTTTGC TCAGGAAGAC
ATTGCCATTT GTAGACCACA CCTTGCCATC AAGATGCCAG AGAAAGCAGA GCAGTATCAA
CCCCTCACAG CCTCAGTCAG CCTCCAGAAC TCCCTAGATG CCCCCATGGA GGACTGTGTG
ATCTCCATCC TGGGAAGGGG GCTCATTCAC AGAGAGAGGA GCTACAGATT CCGTTCAGTG
TGGCCTGAAA ACACCATGTG TGCCAAGTTC CAGTTCACGC CAACACATGT GGGGCTCCAG
AGACTCACTG TGGAAGTGGA CTGCAACATG TTCCAGAACC TAACCAACTA TAAAAGCGTC
ACCGTGGTAG CCCCTGAACT ATCAGCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011519654.1
CDS240..2369
Misc Feature(1)348..698
Misc Feature(2)1074..1349
Misc Feature(3)1716..2033
Misc Feature(4)2055..2351
Translation

Target ORF information:

RefSeq Version XM_011521352.2
Organism Homo sapiens(Human)
Definition Homo sapiens erythrocyte membrane protein band 4.2 (EPB42), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011521352.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGGGACAGG GTGAGCCAAG CCAGCGCTCG ACAGGGCTTG CTGGACTGTA TGCAGCCCCC 
GCAGCATCAC CTGTTTTCAT TAAAGGAAGT GGGATGGATG CCCTGGGTAT CAAGAGCTGT
GACTTTCAGG CAGCAAGAAA CAATGAGGAG CACCACACCA AGGCCCTCAG CTCCCGGCGC
CTCTTTGTGA GGAGGGGGCA GCCCTTCACC ATCATCCTGT ACTTCCGCGC TCCAGTCCGT
GCATTTCTGC CTGCCCTGAA GAAGGTGGCC CTCACTGCAC AAACTGGAGA GCAGCCTTCC
AAGATCAACA GGACCCAAGC CACATTCCCA ATTTCCAGTC TGGGGGACCG AAAGTGGTGG
AGTGCAGTGG TGGAGGAGAG AGATGCCCAG TCCTGGACCA TCTCTGTGAC CACACCTGCG
GACGCTGTCA TTGGCCACTA CTCGCTTCTG CTGCAGGTCT CAGGCAGGAA GCAACTCCTC
TTGGAGGATG CTGTGTTCCT GAAGAATGAG GCTCAGCGCA TGGAGTACTT GTTGAACCAG
AATGGTCTCA TCTACCTGGG TACAGCTGAC TGCATCCAGG CAGAGTCCTG GGACTTTGGC
CAGTTCGAGG GGGATGTCAT TGACCTCAGC CTGCGCTTGC TGAGCAAGGA CAAGCAGGTA
GAGAAGTGGA GCCAGCCGGT GCACGTGGCC CGTGTGTTGG GTGCCTTGCT GCATTTTCTC
AAGGAGCAGA GGGTCCTGCC CACCCCGCAG ACCCAGGCCA CCCAGGAAGG GGCCTTGCTG
AACAAGCGCC GGGGCAGCGT GCCCATCCTG CGGCAGTGGC TCACCGGCCG AGGCCGACCT
GTGTATGATG GCCAGGCCTG GGTGTTGGCT GCTGTTGCTT GCACAGTGCT GCGATGCCTG
GGAATCCCTG CCCGCGTGGT GACCACGTTT GCCTCAGCAC AGGGCACCGG TGGGCGTCTT
CTCATAGATG AATACTATAA TGAGGAGGGA CTTCAGAACG GAGAAGGCCA GAGAGGCAGA
ATCTGGATCT TCCAGACTTC CACAGAGTGC TGGATGACGC GGCCTGCCTT GCCCCAGGGT
TATGATGGAT GGCAGATTCT GCACCCAAGT GCTCCTAATG GAGGTGGAGT CCTGGGGTCC
TGTGATCTGG TGCCGGTCAG AGCAGTCAAG GAGGGGACGC TGGGGCTGAC CCCAGCAGTG
TCAGACCTTT TTGCTGCCAT AAATGCCTCA TGTGTGGTCT GGAAGTGCTG TGAGGATGGG
ACACTGGAGT TGACTGACTC CAACACAAAG TATGTTGGCA ACAACATCAG CACCAAGGGT
GTGGGCAGTG ACCGCTGCGA GGACATCACT CAGAACTACA AGTATCCTGA AGGGTCTCTT
CAGGAAAAAG AGGTGCTGGA GAGAGTCGAG AAAGAGAAAA TGGAACGTGA GAAAGACAAC
GGCATCCGTC CTCCCAGTCT CGAGACTGCC AGTCCTCTGT ACCTGCTCTT GAAAGCACCC
AGCTCCCTAC CCCTGAGAGG GGATGCCCAG ATCTCAGTGA CGCTGGTTAA TCACAGTGAG
CAGGAGAAGG CAGTGCAGCT GGCAATTGGG GTCCAGGCTG TACACTACAA CGGTGTCCTT
GCTGCCAAGC TCTGGAGGAA GAAGCTGCAC CTCACGCTCA GTGCCAACCT GGAAAAGATA
ATAACCATCG GCCTGTTCTT CTCCAATTTT GAGCGAAACC CACCCGAGAA CACCTTCCTT
AGACTCACCG CCATGGCAAC ACACTCTGAA TCCAACCTTA GCTGCTTTGC TCAGGAAGAC
ATTGCCATTT GTAGACCACA CCTTGCCATC AAGATGCCAG AGAAAGCAGA GCAGTATCAA
CCCCTCACAG CCTCAGTCAG CCTCCAGAAC TCCCTAGATG CCCCCATGGA GGACTGTGTG
ATCTCCATCC TGGGAAGGGG GCTCATTCAC AGAGAGAGGA GCTACAGATT CCGTTCAGTG
TGGCCTGAAA ACACCATGTG TGCCAAGTTC CAGTTCACGC CAACACATGT GGGGCTCCAG
AGACTCACTG TGGAAGTGGA CTGCAACATG TTCCAGAACC TAACCAACTA TAAAAGCGTC
ACCGTGGTAG CCCCTGAACT ATCAGCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57539
Clone ID Related Accession (Same CDS sequence) XM_011521353.2
Accession Version XM_011521353.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2061bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product erythrocyte membrane protein band 4.2 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521353.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGGACAGG GTGAGCCAAG CCAGCGCTCG ACAGGGCTTG CTGGACTGTA TGCAGCCCCC 
GCAGCATCAC CTGTTTTCAT TAAAGGAAGT GGGATGGATG CCCTGGGTAT CAAGAGCTGT
GACTTTCAGG CAGCAAGAAA CAATGAGGAG CACCACACCA AGGCCCTCAG CTCCCGGCGC
CTCTTTGTGA GGAGGGGGCA GCCCTTCACC ATCATCCTGT ACTTCCGCGC TCCAGTCCGT
GCATTTCTGC CTGCCCTGAA GAAGGTGGCC CTCACTGCAC AAACTGGAGA GCAGCCTTCC
AAGATCAACA GGACCCAAGC CACATTCCCA ATTTCCAGTC TGGGGGACCG AAAGTGGTGG
AGTGCAGTGG TGGAGGAGAG AGATGCCCAG TCCTGGACCA TCTCTGTGAC CACACCTGCG
GACGCTGTCA TTGGCCACTA CTCGCTTCTG CTGCAGGTCT CAGGCAGGAA GCAACTCCTC
TTGGGTCAGT TCACACTGCT TTTTAACCCC TGGAATAGAG AGGATGCTGT GTTCCTGAAG
AATGAGGCTC AGCGCATGGA GTACTTGTTG AACCAGAATG GTCTCATCTA CCTGGGTACA
GCTGACTGCA TCCAGGCAGA GTCCTGGGAC TTTGGCCAGC TGCATTTTCT CAAGGAGCAG
AGGGTCCTGC CCACCCCGCA GACCCAGGCC ACCCAGGAAG GGGCCTTGCT GAACAAGCGC
CGGGGCAGCG TGCCCATCCT GCGGCAGTGG CTCACCGGCC GAGGCCGACC TGTGTATGAT
GGCCAGGCCT GGGTGTTGGC TGCTGTTGCT TGCACAGTGC TGCGATGCCT GGGAATCCCT
GCCCGCGTGG TGACCACGTT TGCCTCAGCA CAGGGCACCG GTGGGCGTCT TCTCATAGAT
GAATACTATA ATGAGGAGGG ACTTCAGAAC GGAGAAGGCC AGAGAGGCAG AATCTGGATC
TTCCAGACTT CCACAGAGTG CTGGATGACG CGGCCTGCCT TGCCCCAGGG TTATGATGGA
TGGCAGATTC TGCACCCAAG TGCTCCTAAT GGAGGTGGAG TCCTGGGGTC CTGTGATCTG
GTGCCGGTCA GAGCAGTCAA GGAGGGGACG CTGGGGCTGA CCCCAGCAGT GTCAGACCTT
TTTGCTGCCA TAAATGCCTC ATGTGTGGTC TGGAAGTGCT GTGAGGATGG GACACTGGAG
TTGACTGACT CCAACACAAA GTATGTTGGC AACAACATCA GCACCAAGGG TGTGGGCAGT
GACCGCTGCG AGGACATCAC TCAGAACTAC AAGTATCCTG AAGGGTCTCT TCAGGAAAAA
GAGGTGCTGG AGAGAGTCGA GAAAGAGAAA ATGGAACGTG AGAAAGACAA CGGCATCCGT
CCTCCCAGTC TCGAGACTGC CAGTCCTCTG TACCTGCTCT TGAAAGCACC CAGCTCCCTA
CCCCTGAGAG GGGATGCCCA GATCTCAGTG ACGCTGGTTA ATCACAGTGA GCAGGAGAAG
GCAGTGCAGC TGGCAATTGG GGTCCAGGCT GTACACTACA ACGGTGTCCT TGCTGCCAAG
CTCTGGAGGA AGAAGCTGCA CCTCACGCTC AGTGCCAACC TGGAAAAGAT AATAACCATC
GGCCTGTTCT TCTCCAATTT TGAGCGAAAC CCACCCGAGA ACACCTTCCT TAGACTCACC
GCCATGGCAA CACACTCTGA ATCCAACCTT AGCTGCTTTG CTCAGGAAGA CATTGCCATT
TGTAGACCAC ACCTTGCCAT CAAGATGCCA GAGAAAGCAG AGCAGTATCA ACCCCTCACA
GCCTCAGTCA GCCTCCAGAA CTCCCTAGAT GCCCCCATGG AGGACTGTGT GATCTCCATC
CTGGGAAGGG GGCTCATTCA CAGAGAGAGG AGCTACAGAT TCCGTTCAGT GTGGCCTGAA
AACACCATGT GTGCCAAGTT CCAGTTCACG CCAACACATG TGGGGCTCCA GAGACTCACT
GTGGAAGTGG ACTGCAACAT GTTCCAGAAC CTAACCAACT ATAAAAGCGT CACCGTGGTA
GCCCCTGAAC TATCAGCTTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011519655.1
CDS239..2299
Misc Feature(1)347..697
Misc Feature(2)1004..1279
Misc Feature(3)1646..1963
Misc Feature(4)1985..2281
Translation

Target ORF information:

RefSeq Version XM_011521353.2
Organism Homo sapiens(Human)
Definition Homo sapiens erythrocyte membrane protein band 4.2 (EPB42), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011521353.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGGACAGG GTGAGCCAAG CCAGCGCTCG ACAGGGCTTG CTGGACTGTA TGCAGCCCCC 
GCAGCATCAC CTGTTTTCAT TAAAGGAAGT GGGATGGATG CCCTGGGTAT CAAGAGCTGT
GACTTTCAGG CAGCAAGAAA CAATGAGGAG CACCACACCA AGGCCCTCAG CTCCCGGCGC
CTCTTTGTGA GGAGGGGGCA GCCCTTCACC ATCATCCTGT ACTTCCGCGC TCCAGTCCGT
GCATTTCTGC CTGCCCTGAA GAAGGTGGCC CTCACTGCAC AAACTGGAGA GCAGCCTTCC
AAGATCAACA GGACCCAAGC CACATTCCCA ATTTCCAGTC TGGGGGACCG AAAGTGGTGG
AGTGCAGTGG TGGAGGAGAG AGATGCCCAG TCCTGGACCA TCTCTGTGAC CACACCTGCG
GACGCTGTCA TTGGCCACTA CTCGCTTCTG CTGCAGGTCT CAGGCAGGAA GCAACTCCTC
TTGGGTCAGT TCACACTGCT TTTTAACCCC TGGAATAGAG AGGATGCTGT GTTCCTGAAG
AATGAGGCTC AGCGCATGGA GTACTTGTTG AACCAGAATG GTCTCATCTA CCTGGGTACA
GCTGACTGCA TCCAGGCAGA GTCCTGGGAC TTTGGCCAGC TGCATTTTCT CAAGGAGCAG
AGGGTCCTGC CCACCCCGCA GACCCAGGCC ACCCAGGAAG GGGCCTTGCT GAACAAGCGC
CGGGGCAGCG TGCCCATCCT GCGGCAGTGG CTCACCGGCC GAGGCCGACC TGTGTATGAT
GGCCAGGCCT GGGTGTTGGC TGCTGTTGCT TGCACAGTGC TGCGATGCCT GGGAATCCCT
GCCCGCGTGG TGACCACGTT TGCCTCAGCA CAGGGCACCG GTGGGCGTCT TCTCATAGAT
GAATACTATA ATGAGGAGGG ACTTCAGAAC GGAGAAGGCC AGAGAGGCAG AATCTGGATC
TTCCAGACTT CCACAGAGTG CTGGATGACG CGGCCTGCCT TGCCCCAGGG TTATGATGGA
TGGCAGATTC TGCACCCAAG TGCTCCTAAT GGAGGTGGAG TCCTGGGGTC CTGTGATCTG
GTGCCGGTCA GAGCAGTCAA GGAGGGGACG CTGGGGCTGA CCCCAGCAGT GTCAGACCTT
TTTGCTGCCA TAAATGCCTC ATGTGTGGTC TGGAAGTGCT GTGAGGATGG GACACTGGAG
TTGACTGACT CCAACACAAA GTATGTTGGC AACAACATCA GCACCAAGGG TGTGGGCAGT
GACCGCTGCG AGGACATCAC TCAGAACTAC AAGTATCCTG AAGGGTCTCT TCAGGAAAAA
GAGGTGCTGG AGAGAGTCGA GAAAGAGAAA ATGGAACGTG AGAAAGACAA CGGCATCCGT
CCTCCCAGTC TCGAGACTGC CAGTCCTCTG TACCTGCTCT TGAAAGCACC CAGCTCCCTA
CCCCTGAGAG GGGATGCCCA GATCTCAGTG ACGCTGGTTA ATCACAGTGA GCAGGAGAAG
GCAGTGCAGC TGGCAATTGG GGTCCAGGCT GTACACTACA ACGGTGTCCT TGCTGCCAAG
CTCTGGAGGA AGAAGCTGCA CCTCACGCTC AGTGCCAACC TGGAAAAGAT AATAACCATC
GGCCTGTTCT TCTCCAATTT TGAGCGAAAC CCACCCGAGA ACACCTTCCT TAGACTCACC
GCCATGGCAA CACACTCTGA ATCCAACCTT AGCTGCTTTG CTCAGGAAGA CATTGCCATT
TGTAGACCAC ACCTTGCCAT CAAGATGCCA GAGAAAGCAG AGCAGTATCA ACCCCTCACA
GCCTCAGTCA GCCTCCAGAA CTCCCTAGAT GCCCCCATGG AGGACTGTGT GATCTCCATC
CTGGGAAGGG GGCTCATTCA CAGAGAGAGG AGCTACAGAT TCCGTTCAGT GTGGCCTGAA
AACACCATGT GTGCCAAGTT CCAGTTCACG CCAACACATG TGGGGCTCCA GAGACTCACT
GTGGAAGTGG ACTGCAACAT GTTCCAGAAC CTAACCAACT ATAAAAGCGT CACCGTGGTA
GCCCCTGAAC TATCAGCTTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Protein 4.2 interaction with hereditary spherocytosis mutants of the cytoplasmic domain of human anion exchanger 1.
The Biochemical journal433(2)313-22(2011 Jan)
Bustos SP,Reithmeier RA


book

Protein 4.2 binds to the carboxyl-terminal EF-hands of erythroid alpha-spectrin in a calcium- and calmodulin-dependent manner.
The Journal of biological chemistry285(7)4757-70(2010 Feb)
Korsgren C,Peters LL,Lux SE


book

Protein 4.2: a complex linker.
Blood cells, molecules & diseases42(3)201-10(2009 May-Jun)
Satchwell TJ,Shoemark DK,Sessions RB,Toye AM


book

Associations of protein 4.2 with band 3 and ankyrin.
Molecular and cellular biochemistry289(1-2)159-66(2006 Sep)
Su Y,Ding Y,Jiang M,Jiang W,Hu X,Zhang Z


 
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