ANO5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ANO5
Entrez Gene ID	203859
Full Name	anoctamin 5
Synonyms	GDD1,LGMD2L,TMEM16E
General protein information	Preferred Names anoctamin 5 Names anoctamin-5 gnathodiaphyseal dysplasia 1 protein integral membrane protein GDD1 transmembrane protein 16E
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p14.3
Summary	This gene encodes a member of the anoctamin family of transmembrane proteins. The encoded protein is likely a calcium activated chloride channel. Mutations in this gene have been associated with gnathodiaphyseal dysplasia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009].
Disorder MIM:	608662
Disorder Html:	Gnathodiaphyseal dysplasia, 166260 (3); Muscular dystrophy, limb-girdle, type 2L, 611307 (3); Miyoshi muscular dystrophy 3, 613319 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_213599.2	NP_998764.1	anoctamin-5 isoform a
NM_001142649.1	NP_001136121.1	anoctamin-5 isoform b
XM_005252821.3	XP_005252878.2	anoctamin-5 isoform X2
XM_011519949.2	XP_011518251.1	anoctamin-5 isoform X3
XM_005252820.3	XP_005252877.2	anoctamin-5 isoform X1
XM_005252822.4	XP_005252879.1	anoctamin-5 isoform X4
NM_001142649.2	NP_001136121.1	anoctamin-5 isoform b
NM_213599.3	NP_998764.1	anoctamin-5 isoform a

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ano5	XP_006229328.1
Danio rerio (zebrafish)	ano5b	NP_001073452.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010474	XP_311470.4
	LOC704166	XP_002799660.1
Canis lupus familiaris (dog)	ANO5	NP_001161885.1
Homo sapiens (human)	ANO5	NP_001136121.1
Mus musculus (house mouse)	Ano5	NP_808362.2
Danio rerio (zebrafish)	ano5a	XP_005163279.1
Drosophila melanogaster (fruit fly)	CG6938	NP_648535.2
Bos taurus (cattle)	ANO5	NP_001161878.1
Xenopus tropicalis (tropical clawed frog)	ano5	XP_002933920.2
Pan troglodytes (chimpanzee)	ANO5	XP_003313017.1
Gallus gallus (chicken)	ANO5	NP_001161856.1

Gene Ontology
Bibliography

Related articles in PubMed

Gain of function of TMEM16E/ANO5 scrambling activity caused by a mutation associated with gnathodiaphyseal dysplasia.
Di Zanni E, Gradogna A, Scholz-Starke J, Boccaccio A
Cellular and molecular life sciences : CMLS(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Asymptomatic, sometimes mild hyperCKemia or exercise intolerance is a presentation of ANO5-related myopathy.
Title: Hyperckemia and myalgia are common presentations of anoctamin-5-related myopathy in French patients.

Here we show that Ano5-deficient mice have reduced capacity to repair the sarcolemma following laser-induced damage, exhibit delayed regeneration after cardiotoxin injury and suffer from defective myoblast fusion necessary for the proper repair and regeneration of multinucleated myotubes. these data suggest that ANO5 plays an important role in sarcolemmal membrane dynamics.
Title: Defective membrane fusion and repair in Anoctamin5-deficient muscular dystrophy.

Study screened the ANO5 gene in 786 myopathic patients and 52 controls by combining NGS and Sanger sequencing. In the cohort of patients, thirty-three are homozygous or compound heterozygous for causative mutations in ANO5
Title: Next generation sequencing on patients with LGMD and nonspecific myopathies: Findings associated with ANO5 mutations.

supervised aerobic exercise training is safe and effective in improving oxidative capacity and muscle function in patients with anoctamin 5 deficiency
Title: Aerobic training in patients with anoctamin 5 myopathy and hyperckemia.

The present report describes an association between LGMD2L consequent upon mutation in ANO5 and macular dystrophy in one affected person.
Title: Macular dystrophy presenting in one of two siblings with limb-girdle muscular dystrophy type 2L due to mutation of ANO5.

The following ANO5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANO5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26368	NM_213599.3 Latest version!	Homo sapiens anoctamin 5 (ANO5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu26420	NM_001142649.2 Latest version!	Homo sapiens anoctamin 5 (ANO5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$643.30 $919.00
OHu44795	XM_005252822.4 Latest version!	Homo sapiens anoctamin 5 (ANO5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu77856	XM_005252820.3 Latest version!	Homo sapiens anoctamin 5 (ANO5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu77857	XM_005252821.3 Latest version!	Homo sapiens anoctamin 5 (ANO5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu77858	XM_011519949.2 Latest version!	Homo sapiens anoctamin 5 (ANO5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
View Old Accession Versions >>
OHu26420	NM_001142649.1	Homo sapiens anoctamin 5 (ANO5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$643.30 $919.00
OHu26368	NM_213599.2	Homo sapiens anoctamin 5 (ANO5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ANO5 ( NM_213599.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_998764.1 Homo sapiens anoctamin 5 (ANO5), transcript variant 1, mRNA.

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CloneID	OHu26368
Clone ID Related Accession (Same CDS sequence)	NM_213599.2 , NM_213599.3
Accession Version	NM_213599.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	anoctamin-5 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB125267.1, AC107886.2, AK125046.1, AC104009.7, AL833271.1, BU742779.1 and N89918.1. This sequence is a reference standard in the RefSeqGene project. On Dec 16, 2008 this sequence version replaced NM_213599.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL833271.1, SRR1803617.12953.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCGACC CGGATCTCCT GGAAGTGTTG GCGGAGGAAG GGGAAAAAGT CAATAAGCAT 
ATAGACTACT CTTTCCAAAT GAGTGAGCAG AGCCTGAGCA GCAGAGAGAC CAGCTTTCTC 
ATCAATGAAG AAACAATGCC TGCAAAGCGA TTCAATTTGT TCCTGAGGCG GCGGCTTATG 
TTTCAAAAAA ATCAGCAAAG CAAAGATTCT ATCTTCTTCC GAGATGGGAT TAGGCAAATT 
GATTTTGTGC TTTCCTACGT TGATGATGTA AAGAAAGACG CAGAGTTAAA GGCGGAAAGA 
AGAAAAGAGT TTGAAACTAA TCTCAGAAAA ACAGGTCTTG AGTTGGAAAT AGAAGACAAA 
AGGGACTCGG AAGATGGAAG AACTTATTTT GTCAAGATCC ATGCCCCTTG GGAGGTATTA 
GTTACCTATG CTGAAGTCTT GGGAATCAAA ATGCCTATTA AGGAGAGTGA TATTCCCCGC 
CCTAAGCACA CTCCTATAAG CTATGTGCTT GGACCTGTAA GACTCCCACT GAGTGTGAAG 
TATCCCCATC CTGAATATTT TACTGCACAA TTCAGCAGAC ATCGGCAGGA GCTCTTCCTC 
ATCGAAGATC AGGCAACCTT CTTTCCATCC TCATCAAGAA ACAGAATTGT GTACTATATT 
CTCTCAAGAT GTCCTTTTGG CATAGAAGAT GGGAAGAAAA GGTTTGGGAT TGAAAGACTG 
CTAAACTCTA ACACTTACTC ATCTGCCTAT CCACTCCATG ATGGCCAATA TTGGAAGCCA 
TCAGAACCTC CCAATCCTAC CAATGAAAGA TACACACTTC ACCAGAATTG GGCTCGATTT 
TCCTATTTCT ACAAGGAGCA GCCTTTAGAC TTGATTAAGA ATTATTATGG AGAAAAAATT 
GGTATCTATT TTGTCTTTCT TGGATTTTAC ACAGAAATGC TATTCTTTGC AGCTGTAGTT 
GGCTTAGCTT GTTTTATTTA TGGCTTATTA TCAATGGAAC ATAACACAAG CAGCACTGAA 
ATCTGTGACC CTGAGATTGG TGGTCAGATG ATCATGTGCC CACTCTGTGA TCAAGTGTGT 
GATTATTGGA GACTAAATAG TACGTGTTTG GCTTCAAAGT TCTCCCATTT GTTTGATAAT 
GAGTCAACAG TGTTCTTTGC AATATTCATG GGAATTTGGG TCACCTTATT TTTGGAGTTT 
TGGAAACAAC GACAAGCCAG ACTGGAATAT GAATGGGACC TGGTGGACTT TGAAGAGGAA 
CAGCAGCAGC TTCAGCTGAG ACCAGAATTT GAAGCTATGT GTAAACACAG GAAATTGAAT 
GCAGTGACTA AGGAGATGGA ACCTTACATG CCTCTATACA CGCGTATTCC ATGGTACTTT 
CTTTCAGGAG CCACAGTGAC ATTATGGATG TCTCTTGTCG TCACCAGTAT GGTAGCTGTA 
ATTGTGTACC GCCTGTCAGT CTTTGCTACA TTTGCTAGTT TCATGGAAAG TGATGCATCC 
TTAAAGCAGG TCAAAAGCTT CCTTACTCCT CAGATAACCA CATCACTCAC AGGATCATGC 
TTGAACTTTA TTGTCATCTT GATCTTGAAT TTCTTTTATG AAAAGATATC TGCCTGGATC 
ACAAAAATGG AAATTCCTCG AACATACCAG GAGTATGAGA GCAGTCTTAC CTTGAAAATG 
TTCCTGTTTC AGTTTGTAAA TTTTTACTCA TCCTGCTTCT ACGTAGCTTT CTTTAAAGGG 
AAGTTCGTAG GCTATCCTGG AAAATACACA TATTTATTTA ATGAGTGGAG AAGTGAAGAG 
TGTGATCCTG GAGGCTGTCT TATAGAATTG ACAACCCAAT TGACCATTAT AATGACCGGG 
AAACAGATTT TTGGAAACAT TAAAGAAGCC ATTTATCCCT TGGCTTTGAA TTGGTGGAGA 
CGCCGAAAAG CTCGGACAAA CTCTGAGAAG CTGTATAGTC GATGGGAGCA GGATCATGAC 
CTTGAAAGTT TTGGACCCCT TGGGCTTTTC TATGAGTACT TAGAAACAGT TACTCAATTT 
GGATTTGTTA CACTATTTGT GGCCTCTTTT CCTTTGGCTC CTCTTCTTGC TCTCATAAAT 
AATATTGTAG AGATTCGAGT GGATGCCTGG AAACTTACCA CTCAATACAG GAGAACTGTA 
GCTTCTAAAG CTCATAGCAT AGGTGTTTGG CAAGACATTC TTTATGGAAT GGCTGTCCTT 
TCTGTTGCAA CTAATGCCTT TATTGTTGCA TTTACGTCAG ACATCATTCC CCGTCTAGTT 
TACTACTATG CTTACTCAAC AAATGCCACA CAGCCTATGA CAGGATATGT GAATAATAGC 
CTGTCAGTAT TCCTGATAGC TGATTTTCCA AACCACACTG CACCTTCGGA AAAACGAGAC 
TTCATCACTT GCAGGTACAG AGATTACAGA TATCCTCCTG ATGACGAGAA TAAATATTTT 
CATAATATGC AATTCTGGCA TGTCCTTGCT GCCAAGATGA CCTTCATCAT TGTTATGGAA 
CATGTTGTGT TTTTAGTTAA ATTTTTGCTG GCCTGGATGA TACCTGATGT TCCAAAAGAT 
GTTGTGGAGA GAATCAAGAG AGAAAAGTTA ATGACTATCA AGATTCTCCA TGATTTTGAG 
CTCAACAAAT TAAAAGAGAA CTTGGGAATT AATTCTAATG AATTTGCCAA GCATGTCATG 
ATTGAGGAAA ACAAAGCACA GCTGGCTAAA TCAACACTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998764.1
CDS	318..3059
Translation	MGDPDLLEVLAEEGEKVNKHIDYSFQMSEQSLSSRETSFLINEETMPAKRFNLFLRRRLMFQKNQQSKDSIFFRDGIRQIDFVLSYVDDVKKDAELKAERRKEFETNLRKTGLELEIEDKRDSEDGRTYFVKIHAPWEVLVTYAEVLGIKMPIKESDIPRPKHTPISYVLGPVRLPLSVKYPHPEYFTAQFSRHRQELFLIEDQATFFPSSSRNRIVYYILSRCPFGIEDGKKRFGIERLLNSNTYSSAYPLHDGQYWKPSEPPNPTNERYTLHQNWARFSYFYKEQPLDLIKNYYGEKIGIYFVFLGFYTEMLFFAAVVGLACFIYGLLSMEHNTSSTEICDPEIGGQMIMCPLCDQVCDYWRLNSTCLASKFSHLFDNESTVFFAIFMGIWVTLFLEFWKQRQARLEYEWDLVDFEEEQQQLQLRPEFEAMCKHRKLNAVTKEMEPYMPLYTRIPWYFLSGATVTLWMSLVVTSMVAVIVYRLSVFATFASFMESDASLKQVKSFLTPQITTSLTGSCLNFIVILILNFFYEKISAWITKMEIPRTYQEYESSLTLKMFLFQFVNFYSSCFYVAFFKGKFVGYPGKYTYLFNEWRSEECDPGGCLIELTTQLTIIMTGKQIFGNIKEAIYPLALNWWRRRKARTNSEKLYSRWEQDHDLESFGPLGLFYEYLETVTQFGFVTLFVASFPLAPLLALINNIVEIRVDAWKLTTQYRRTVASKAHSIGVWQDILYGMAVLSVATNAFIVAFTSDIIPRLVYYYAYSTNATQPMTGYVNNSLSVFLIADFPNHTAPSEKRDFITCRYRDYRYPPDDENKYFHNMQFWHVLAAKMTFIIVMEHVVFLVKFLLAWMIPDVPKDVVERIKREKLMTIKILHDFELNKLKENLGINSNEFAKHVMIEENKAQLAKSTL

Target ORF information:

RefSeq Version	NM_213599.2
Organism	Homo sapiens(human)
Definition	Homo sapiens anoctamin 5 (ANO5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213599.2

ORF Insert Sequence:

ATGGGCGACC CGGATCTCCT GGAAGTGTTG GCGGAGGAAG GGGAAAAAGT CAATAAGCAT 
ATAGACTACT CTTTCCAAAT GAGTGAGCAG AGCCTGAGCA GCAGAGAGAC CAGCTTTCTC 
ATCAATGAAG AAACAATGCC TGCAAAGCGA TTCAATTTGT TCCTGAGGCG GCGGCTTATG 
TTTCAAAAAA ATCAGCAAAG CAAAGATTCT ATCTTCTTCC GAGATGGGAT TAGGCAAATT 
GATTTTGTGC TTTCCTACGT TGATGATGTA AAGAAAGACG CAGAGTTAAA GGCGGAAAGA 
AGAAAAGAGT TTGAAACTAA TCTCAGAAAA ACAGGTCTTG AGTTGGAAAT AGAAGACAAA 
AGGGACTCGG AAGATGGAAG AACTTATTTT GTCAAGATCC ATGCCCCTTG GGAGGTATTA 
GTTACCTATG CTGAAGTCTT GGGAATCAAA ATGCCTATTA AGGAGAGTGA TATTCCCCGC 
CCTAAGCACA CTCCTATAAG CTATGTGCTT GGACCTGTAA GACTCCCACT GAGTGTGAAG 
TATCCCCATC CTGAATATTT TACTGCACAA TTCAGCAGAC ATCGGCAGGA GCTCTTCCTC 
ATCGAAGATC AGGCAACCTT CTTTCCATCC TCATCAAGAA ACAGAATTGT GTACTATATT 
CTCTCAAGAT GTCCTTTTGG CATAGAAGAT GGGAAGAAAA GGTTTGGGAT TGAAAGACTG 
CTAAACTCTA ACACTTACTC ATCTGCCTAT CCACTCCATG ATGGCCAATA TTGGAAGCCA 
TCAGAACCTC CCAATCCTAC CAATGAAAGA TACACACTTC ACCAGAATTG GGCTCGATTT 
TCCTATTTCT ACAAGGAGCA GCCTTTAGAC TTGATTAAGA ATTATTATGG AGAAAAAATT 
GGTATCTATT TTGTCTTTCT TGGATTTTAC ACAGAAATGC TATTCTTTGC AGCTGTAGTT 
GGCTTAGCTT GTTTTATTTA TGGCTTATTA TCAATGGAAC ATAACACAAG CAGCACTGAA 
ATCTGTGACC CTGAGATTGG TGGTCAGATG ATCATGTGCC CACTCTGTGA TCAAGTGTGT 
GATTATTGGA GACTAAATAG TACGTGTTTG GCTTCAAAGT TCTCCCATTT GTTTGATAAT 
GAGTCAACAG TGTTCTTTGC AATATTCATG GGAATTTGGG TCACCTTATT TTTGGAGTTT 
TGGAAACAAC GACAAGCCAG ACTGGAATAT GAATGGGACC TGGTGGACTT TGAAGAGGAA 
CAGCAGCAGC TTCAGCTGAG ACCAGAATTT GAAGCTATGT GTAAACACAG GAAATTGAAT 
GCAGTGACTA AGGAGATGGA ACCTTACATG CCTCTATACA CGCGTATTCC ATGGTACTTT 
CTTTCAGGAG CCACAGTGAC ATTATGGATG TCTCTTGTCG TCACCAGTAT GGTAGCTGTA 
ATTGTGTACC GCCTGTCAGT CTTTGCTACA TTTGCTAGTT TCATGGAAAG TGATGCATCC 
TTAAAGCAGG TCAAAAGCTT CCTTACTCCT CAGATAACCA CATCACTCAC AGGATCATGC 
TTGAACTTTA TTGTCATCTT GATCTTGAAT TTCTTTTATG AAAAGATATC TGCCTGGATC 
ACAAAAATGG AAATTCCTCG AACATACCAG GAGTATGAGA GCAGTCTTAC CTTGAAAATG 
TTCCTGTTTC AGTTTGTAAA TTTTTACTCA TCCTGCTTCT ACGTAGCTTT CTTTAAAGGG 
AAGTTCGTAG GCTATCCTGG AAAATACACA TATTTATTTA ATGAGTGGAG AAGTGAAGAG 
TGTGATCCTG GAGGCTGTCT TATAGAATTG ACAACCCAAT TGACCATTAT AATGACCGGG 
AAACAGATTT TTGGAAACAT TAAAGAAGCC ATTTATCCCT TGGCTTTGAA TTGGTGGAGA 
CGCCGAAAAG CTCGGACAAA CTCTGAGAAG CTGTATAGTC GATGGGAGCA GGATCATGAC 
CTTGAAAGTT TTGGACCCCT TGGGCTTTTC TATGAGTACT TAGAAACAGT TACTCAATTT 
GGATTTGTTA CACTATTTGT GGCCTCTTTT CCTTTGGCTC CTCTTCTTGC TCTCATAAAT 
AATATTGTAG AGATTCGAGT GGATGCCTGG AAACTTACCA CTCAATACAG GAGAACTGTA 
GCTTCTAAAG CTCATAGCAT AGGTGTTTGG CAAGACATTC TTTATGGAAT GGCTGTCCTT 
TCTGTTGCAA CTAATGCCTT TATTGTTGCA TTTACGTCAG ACATCATTCC CCGTCTAGTT 
TACTACTATG CTTACTCAAC AAATGCCACA CAGCCTATGA CAGGATATGT GAATAATAGC 
CTGTCAGTAT TCCTGATAGC TGATTTTCCA AACCACACTG CACCTTCGGA AAAACGAGAC 
TTCATCACTT GCAGGTACAG AGATTACAGA TATCCTCCTG ATGACGAGAA TAAATATTTT 
CATAATATGC AATTCTGGCA TGTCCTTGCT GCCAAGATGA CCTTCATCAT TGTTATGGAA 
CATGTTGTGT TTTTAGTTAA ATTTTTGCTG GCCTGGATGA TACCTGATGT TCCAAAAGAT 
GTTGTGGAGA GAATCAAGAG AGAAAAGTTA ATGACTATCA AGATTCTCCA TGATTTTGAG 
CTCAACAAAT TAAAAGAGAA CTTGGGAATT AATTCTAATG AATTTGCCAA GCATGTCATG 
ATTGAGGAAA ACAAAGCACA GCTGGCTAAA TCAACACTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANO5 ( NM_213599.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_998764.1 Homo sapiens anoctamin 5 (ANO5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26368
Clone ID Related Accession (Same CDS sequence)	NM_213599.2 , NM_213599.3
Accession Version	NM_213599.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	anoctamin-5 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107886.2, AB125267.1, AK125046.1, AC104009.7, AL833271.1 and BU742779.1. On Dec 4, 2019 this sequence version replaced NM_213599.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL833271.1, AB125267.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCGACC CGGATCTCCT GGAAGTGTTG GCGGAGGAAG GGGAAAAAGT CAATAAGCAT 
ATAGACTACT CTTTCCAAAT GAGTGAGCAG AGCCTGAGCA GCAGAGAGAC CAGCTTTCTC 
ATCAATGAAG AAACAATGCC TGCAAAGCGA TTCAATTTGT TCCTGAGGCG GCGGCTTATG 
TTTCAAAAAA ATCAGCAAAG CAAAGATTCT ATCTTCTTCC GAGATGGGAT TAGGCAAATT 
GATTTTGTGC TTTCCTACGT TGATGATGTA AAGAAAGACG CAGAGTTAAA GGCGGAAAGA 
AGAAAAGAGT TTGAAACTAA TCTCAGAAAA ACAGGTCTTG AGTTGGAAAT AGAAGACAAA 
AGGGACTCGG AAGATGGAAG AACTTATTTT GTCAAGATCC ATGCCCCTTG GGAGGTATTA 
GTTACCTATG CTGAAGTCTT GGGAATCAAA ATGCCTATTA AGGAGAGTGA TATTCCCCGC 
CCTAAGCACA CTCCTATAAG CTATGTGCTT GGACCTGTAA GACTCCCACT GAGTGTGAAG 
TATCCCCATC CTGAATATTT TACTGCACAA TTCAGCAGAC ATCGGCAGGA GCTCTTCCTC 
ATCGAAGATC AGGCAACCTT CTTTCCATCC TCATCAAGAA ACAGAATTGT GTACTATATT 
CTCTCAAGAT GTCCTTTTGG CATAGAAGAT GGGAAGAAAA GGTTTGGGAT TGAAAGACTG 
CTAAACTCTA ACACTTACTC ATCTGCCTAT CCACTCCATG ATGGCCAATA TTGGAAGCCA 
TCAGAACCTC CCAATCCTAC CAATGAAAGA TACACACTTC ACCAGAATTG GGCTCGATTT 
TCCTATTTCT ACAAGGAGCA GCCTTTAGAC TTGATTAAGA ATTATTATGG AGAAAAAATT 
GGTATCTATT TTGTCTTTCT TGGATTTTAC ACAGAAATGC TATTCTTTGC AGCTGTAGTT 
GGCTTAGCTT GTTTTATTTA TGGCTTATTA TCAATGGAAC ATAACACAAG CAGCACTGAA 
ATCTGTGACC CTGAGATTGG TGGTCAGATG ATCATGTGCC CACTCTGTGA TCAAGTGTGT 
GATTATTGGA GACTAAATAG TACGTGTTTG GCTTCAAAGT TCTCCCATTT GTTTGATAAT 
GAGTCAACAG TGTTCTTTGC AATATTCATG GGAATTTGGG TCACCTTATT TTTGGAGTTT 
TGGAAACAAC GACAAGCCAG ACTGGAATAT GAATGGGACC TGGTGGACTT TGAAGAGGAA 
CAGCAGCAGC TTCAGCTGAG ACCAGAATTT GAAGCTATGT GTAAACACAG GAAATTGAAT 
GCAGTGACTA AGGAGATGGA ACCTTACATG CCTCTATACA CGCGTATTCC ATGGTACTTT 
CTTTCAGGAG CCACAGTGAC ATTATGGATG TCTCTTGTCG TCACCAGTAT GGTAGCTGTA 
ATTGTGTACC GCCTGTCAGT CTTTGCTACA TTTGCTAGTT TCATGGAAAG TGATGCATCC 
TTAAAGCAGG TCAAAAGCTT CCTTACTCCT CAGATAACCA CATCACTCAC AGGATCATGC 
TTGAACTTTA TTGTCATCTT GATCTTGAAT TTCTTTTATG AAAAGATATC TGCCTGGATC 
ACAAAAATGG AAATTCCTCG AACATACCAG GAGTATGAGA GCAGTCTTAC CTTGAAAATG 
TTCCTGTTTC AGTTTGTAAA TTTTTACTCA TCCTGCTTCT ACGTAGCTTT CTTTAAAGGG 
AAGTTCGTAG GCTATCCTGG AAAATACACA TATTTATTTA ATGAGTGGAG AAGTGAAGAG 
TGTGATCCTG GAGGCTGTCT TATAGAATTG ACAACCCAAT TGACCATTAT AATGACCGGG 
AAACAGATTT TTGGAAACAT TAAAGAAGCC ATTTATCCCT TGGCTTTGAA TTGGTGGAGA 
CGCCGAAAAG CTCGGACAAA CTCTGAGAAG CTGTATAGTC GATGGGAGCA GGATCATGAC 
CTTGAAAGTT TTGGACCCCT TGGGCTTTTC TATGAGTACT TAGAAACAGT TACTCAATTT 
GGATTTGTTA CACTATTTGT GGCCTCTTTT CCTTTGGCTC CTCTTCTTGC TCTCATAAAT 
AATATTGTAG AGATTCGAGT GGATGCCTGG AAACTTACCA CTCAATACAG GAGAACTGTA 
GCTTCTAAAG CTCATAGCAT AGGTGTTTGG CAAGACATTC TTTATGGAAT GGCTGTCCTT 
TCTGTTGCAA CTAATGCCTT TATTGTTGCA TTTACGTCAG ACATCATTCC CCGTCTAGTT 
TACTACTATG CTTACTCAAC AAATGCCACA CAGCCTATGA CAGGATATGT GAATAATAGC 
CTGTCAGTAT TCCTGATAGC TGATTTTCCA AACCACACTG CACCTTCGGA AAAACGAGAC 
TTCATCACTT GCAGGTACAG AGATTACAGA TATCCTCCTG ATGACGAGAA TAAATATTTT 
CATAATATGC AATTCTGGCA TGTCCTTGCT GCCAAGATGA CCTTCATCAT TGTTATGGAA 
CATGTTGTGT TTTTAGTTAA ATTTTTGCTG GCCTGGATGA TACCTGATGT TCCAAAAGAT 
GTTGTGGAGA GAATCAAGAG AGAAAAGTTA ATGACTATCA AGATTCTCCA TGATTTTGAG 
CTCAACAAAT TAAAAGAGAA CTTGGGAATT AATTCTAATG AATTTGCCAA GCATGTCATG 
ATTGAGGAAA ACAAAGCACA GCTGGCTAAA TCAACACTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998764.1
CDS	409..3150
Translation	MGDPDLLEVLAEEGEKVNKHIDYSFQMSEQSLSSRETSFLINEETMPAKRFNLFLRRRLMFQKNQQSKDSIFFRDGIRQIDFVLSYVDDVKKDAELKAERRKEFETNLRKTGLELEIEDKRDSEDGRTYFVKIHAPWEVLVTYAEVLGIKMPIKESDIPRPKHTPISYVLGPVRLPLSVKYPHPEYFTAQFSRHRQELFLIEDQATFFPSSSRNRIVYYILSRCPFGIEDGKKRFGIERLLNSNTYSSAYPLHDGQYWKPSEPPNPTNERYTLHQNWARFSYFYKEQPLDLIKNYYGEKIGIYFVFLGFYTEMLFFAAVVGLACFIYGLLSMEHNTSSTEICDPEIGGQMIMCPLCDQVCDYWRLNSTCLASKFSHLFDNESTVFFAIFMGIWVTLFLEFWKQRQARLEYEWDLVDFEEEQQQLQLRPEFEAMCKHRKLNAVTKEMEPYMPLYTRIPWYFLSGATVTLWMSLVVTSMVAVIVYRLSVFATFASFMESDASLKQVKSFLTPQITTSLTGSCLNFIVILILNFFYEKISAWITKMEIPRTYQEYESSLTLKMFLFQFVNFYSSCFYVAFFKGKFVGYPGKYTYLFNEWRSEECDPGGCLIELTTQLTIIMTGKQIFGNIKEAIYPLALNWWRRRKARTNSEKLYSRWEQDHDLESFGPLGLFYEYLETVTQFGFVTLFVASFPLAPLLALINNIVEIRVDAWKLTTQYRRTVASKAHSIGVWQDILYGMAVLSVATNAFIVAFTSDIIPRLVYYYAYSTNATQPMTGYVNNSLSVFLIADFPNHTAPSEKRDFITCRYRDYRYPPDDENKYFHNMQFWHVLAAKMTFIIVMEHVVFLVKFLLAWMIPDVPKDVVERIKREKLMTIKILHDFELNKLKENLGINSNEFAKHVMIEENKAQLAKSTL

Target ORF information:

RefSeq Version	NM_213599.3
Organism	Homo sapiens(human)
Definition	Homo sapiens anoctamin 5 (ANO5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213599.3

ORF Insert Sequence:

ATGGGCGACC CGGATCTCCT GGAAGTGTTG GCGGAGGAAG GGGAAAAAGT CAATAAGCAT 
ATAGACTACT CTTTCCAAAT GAGTGAGCAG AGCCTGAGCA GCAGAGAGAC CAGCTTTCTC 
ATCAATGAAG AAACAATGCC TGCAAAGCGA TTCAATTTGT TCCTGAGGCG GCGGCTTATG 
TTTCAAAAAA ATCAGCAAAG CAAAGATTCT ATCTTCTTCC GAGATGGGAT TAGGCAAATT 
GATTTTGTGC TTTCCTACGT TGATGATGTA AAGAAAGACG CAGAGTTAAA GGCGGAAAGA 
AGAAAAGAGT TTGAAACTAA TCTCAGAAAA ACAGGTCTTG AGTTGGAAAT AGAAGACAAA 
AGGGACTCGG AAGATGGAAG AACTTATTTT GTCAAGATCC ATGCCCCTTG GGAGGTATTA 
GTTACCTATG CTGAAGTCTT GGGAATCAAA ATGCCTATTA AGGAGAGTGA TATTCCCCGC 
CCTAAGCACA CTCCTATAAG CTATGTGCTT GGACCTGTAA GACTCCCACT GAGTGTGAAG 
TATCCCCATC CTGAATATTT TACTGCACAA TTCAGCAGAC ATCGGCAGGA GCTCTTCCTC 
ATCGAAGATC AGGCAACCTT CTTTCCATCC TCATCAAGAA ACAGAATTGT GTACTATATT 
CTCTCAAGAT GTCCTTTTGG CATAGAAGAT GGGAAGAAAA GGTTTGGGAT TGAAAGACTG 
CTAAACTCTA ACACTTACTC ATCTGCCTAT CCACTCCATG ATGGCCAATA TTGGAAGCCA 
TCAGAACCTC CCAATCCTAC CAATGAAAGA TACACACTTC ACCAGAATTG GGCTCGATTT 
TCCTATTTCT ACAAGGAGCA GCCTTTAGAC TTGATTAAGA ATTATTATGG AGAAAAAATT 
GGTATCTATT TTGTCTTTCT TGGATTTTAC ACAGAAATGC TATTCTTTGC AGCTGTAGTT 
GGCTTAGCTT GTTTTATTTA TGGCTTATTA TCAATGGAAC ATAACACAAG CAGCACTGAA 
ATCTGTGACC CTGAGATTGG TGGTCAGATG ATCATGTGCC CACTCTGTGA TCAAGTGTGT 
GATTATTGGA GACTAAATAG TACGTGTTTG GCTTCAAAGT TCTCCCATTT GTTTGATAAT 
GAGTCAACAG TGTTCTTTGC AATATTCATG GGAATTTGGG TCACCTTATT TTTGGAGTTT 
TGGAAACAAC GACAAGCCAG ACTGGAATAT GAATGGGACC TGGTGGACTT TGAAGAGGAA 
CAGCAGCAGC TTCAGCTGAG ACCAGAATTT GAAGCTATGT GTAAACACAG GAAATTGAAT 
GCAGTGACTA AGGAGATGGA ACCTTACATG CCTCTATACA CGCGTATTCC ATGGTACTTT 
CTTTCAGGAG CCACAGTGAC ATTATGGATG TCTCTTGTCG TCACCAGTAT GGTAGCTGTA 
ATTGTGTACC GCCTGTCAGT CTTTGCTACA TTTGCTAGTT TCATGGAAAG TGATGCATCC 
TTAAAGCAGG TCAAAAGCTT CCTTACTCCT CAGATAACCA CATCACTCAC AGGATCATGC 
TTGAACTTTA TTGTCATCTT GATCTTGAAT TTCTTTTATG AAAAGATATC TGCCTGGATC 
ACAAAAATGG AAATTCCTCG AACATACCAG GAGTATGAGA GCAGTCTTAC CTTGAAAATG 
TTCCTGTTTC AGTTTGTAAA TTTTTACTCA TCCTGCTTCT ACGTAGCTTT CTTTAAAGGG 
AAGTTCGTAG GCTATCCTGG AAAATACACA TATTTATTTA ATGAGTGGAG AAGTGAAGAG 
TGTGATCCTG GAGGCTGTCT TATAGAATTG ACAACCCAAT TGACCATTAT AATGACCGGG 
AAACAGATTT TTGGAAACAT TAAAGAAGCC ATTTATCCCT TGGCTTTGAA TTGGTGGAGA 
CGCCGAAAAG CTCGGACAAA CTCTGAGAAG CTGTATAGTC GATGGGAGCA GGATCATGAC 
CTTGAAAGTT TTGGACCCCT TGGGCTTTTC TATGAGTACT TAGAAACAGT TACTCAATTT 
GGATTTGTTA CACTATTTGT GGCCTCTTTT CCTTTGGCTC CTCTTCTTGC TCTCATAAAT 
AATATTGTAG AGATTCGAGT GGATGCCTGG AAACTTACCA CTCAATACAG GAGAACTGTA 
GCTTCTAAAG CTCATAGCAT AGGTGTTTGG CAAGACATTC TTTATGGAAT GGCTGTCCTT 
TCTGTTGCAA CTAATGCCTT TATTGTTGCA TTTACGTCAG ACATCATTCC CCGTCTAGTT 
TACTACTATG CTTACTCAAC AAATGCCACA CAGCCTATGA CAGGATATGT GAATAATAGC 
CTGTCAGTAT TCCTGATAGC TGATTTTCCA AACCACACTG CACCTTCGGA AAAACGAGAC 
TTCATCACTT GCAGGTACAG AGATTACAGA TATCCTCCTG ATGACGAGAA TAAATATTTT 
CATAATATGC AATTCTGGCA TGTCCTTGCT GCCAAGATGA CCTTCATCAT TGTTATGGAA 
CATGTTGTGT TTTTAGTTAA ATTTTTGCTG GCCTGGATGA TACCTGATGT TCCAAAAGAT 
GTTGTGGAGA GAATCAAGAG AGAAAAGTTA ATGACTATCA AGATTCTCCA TGATTTTGAG 
CTCAACAAAT TAAAAGAGAA CTTGGGAATT AATTCTAATG AATTTGCCAA GCATGTCATG 
ATTGAGGAAA ACAAAGCACA GCTGGCTAAA TCAACACTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANO5 ( NM_001142649.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001136121.1 Homo sapiens anoctamin 5 (ANO5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26420
Clone ID Related Accession (Same CDS sequence)	NM_001142649.1 , NM_001142649.2
Accession Version	NM_001142649.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Homo sapiens(human)
Product	anoctamin-5 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL832374.1, AB125267.1, AC107886.2, AK125046.1, AC104009.7, AL833271.1, BU742779.1 and N89918.1. Transcript Variant: This variant (2) lacks an alternate in-frame segment, compared to variant 1, resulting in a shorter protein (isoform b), compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL832374.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGACC CGGATCTCCT GGAAGTGTTG GCGGAGGAAG GGGAAAAAGT CAATAAGCAT 
ATAGACTACT CTTTCCAAAT GAGTGAGAGC CTGAGCAGCA GAGAGACCAG CTTTCTCATC 
AATGAAGAAA CAATGCCTGC AAAGCGATTC AATTTGTTCC TGAGGCGGCG GCTTATGTTT 
CAAAAAAATC AGCAAAGCAA AGATTCTATC TTCTTCCGAG ATGGGATTAG GCAAATTGAT 
TTTGTGCTTT CCTACGTTGA TGATGTAAAG AAAGACGCAG AGTTAAAGGC GGAAAGAAGA 
AAAGAGTTTG AAACTAATCT CAGAAAAACA GGTCTTGAGT TGGAAATAGA AGACAAAAGG 
GACTCGGAAG ATGGAAGAAC TTATTTTGTC AAGATCCATG CCCCTTGGGA GGTATTAGTT 
ACCTATGCTG AAGTCTTGGG AATCAAAATG CCTATTAAGG AGAGTGATAT TCCCCGCCCT 
AAGCACACTC CTATAAGCTA TGTGCTTGGA CCTGTAAGAC TCCCACTGAG TGTGAAGTAT 
CCCCATCCTG AATATTTTAC TGCACAATTC AGCAGACATC GGCAGGAGCT CTTCCTCATC 
GAAGATCAGG CAACCTTCTT TCCATCCTCA TCAAGAAACA GAATTGTGTA CTATATTCTC 
TCAAGATGTC CTTTTGGCAT AGAAGATGGG AAGAAAAGGT TTGGGATTGA AAGACTGCTA 
AACTCTAACA CTTACTCATC TGCCTATCCA CTCCATGATG GCCAATATTG GAAGCCATCA 
GAACCTCCCA ATCCTACCAA TGAAAGATAC ACACTTCACC AGAATTGGGC TCGATTTTCC 
TATTTCTACA AGGAGCAGCC TTTAGACTTG ATTAAGAATT ATTATGGAGA AAAAATTGGT 
ATCTATTTTG TCTTTCTTGG ATTTTACACA GAAATGCTAT TCTTTGCAGC TGTAGTTGGC 
TTAGCTTGTT TTATTTATGG CTTATTATCA ATGGAACATA ACACAAGCAG CACTGAAATC 
TGTGACCCTG AGATTGGTGG TCAGATGATC ATGTGCCCAC TCTGTGATCA AGTGTGTGAT 
TATTGGAGAC TAAATAGTAC GTGTTTGGCT TCAAAGTTCT CCCATTTGTT TGATAATGAG 
TCAACAGTGT TCTTTGCAAT ATTCATGGGA ATTTGGGTCA CCTTATTTTT GGAGTTTTGG 
AAACAACGAC AAGCCAGACT GGAATATGAA TGGGACCTGG TGGACTTTGA AGAGGAACAG 
CAGCAGCTTC AGCTGAGACC AGAATTTGAA GCTATGTGTA AACACAGGAA ATTGAATGCA 
GTGACTAAGG AGATGGAACC TTACATGCCT CTATACACGC GTATTCCATG GTACTTTCTT 
TCAGGAGCCA CAGTGACATT ATGGATGTCT CTTGTCGTCA CCAGTATGGT AGCTGTAATT 
GTGTACCGCC TGTCAGTCTT TGCTACATTT GCTAGTTTCA TGGAAAGTGA TGCATCCTTA 
AAGCAGGTCA AAAGCTTCCT TACTCCTCAG ATAACCACAT CACTCACAGG ATCATGCTTG 
AACTTTATTG TCATCTTGAT CTTGAATTTC TTTTATGAAA AGATATCTGC CTGGATCACA 
AAAATGGAAA TTCCTCGAAC ATACCAGGAG TATGAGAGCA GTCTTACCTT GAAAATGTTC 
CTGTTTCAGT TTGTAAATTT TTACTCATCC TGCTTCTACG TAGCTTTCTT TAAAGGGAAG 
TTCGTAGGCT ATCCTGGAAA ATACACATAT TTATTTAATG AGTGGAGAAG TGAAGAGTGT 
GATCCTGGAG GCTGTCTTAT AGAATTGACA ACCCAATTGA CCATTATAAT GACCGGGAAA 
CAGATTTTTG GAAACATTAA AGAAGCCATT TATCCCTTGG CTTTGAATTG GTGGAGACGC 
CGAAAAGCTC GGACAAACTC TGAGAAGCTG TATAGTCGAT GGGAGCAGGA TCATGACCTT 
GAAAGTTTTG GACCCCTTGG GCTTTTCTAT GAGTACTTAG AAACAGTTAC TCAATTTGGA 
TTTGTTACAC TATTTGTGGC CTCTTTTCCT TTGGCTCCTC TTCTTGCTCT CATAAATAAT 
ATTGTAGAGA TTCGAGTGGA TGCCTGGAAA CTTACCACTC AATACAGGAG AACTGTAGCT 
TCTAAAGCTC ATAGCATAGG TGTTTGGCAA GACATTCTTT ATGGAATGGC TGTCCTTTCT 
GTTGCAACTA ATGCCTTTAT TGTTGCATTT ACGTCAGACA TCATTCCCCG TCTAGTTTAC 
TACTATGCTT ACTCAACAAA TGCCACACAG CCTATGACAG GATATGTGAA TAATAGCCTG 
TCAGTATTCC TGATAGCTGA TTTTCCAAAC CACACTGCAC CTTCGGAAAA ACGAGACTTC 
ATCACTTGCA GGTACAGAGA TTACAGATAT CCTCCTGATG ACGAGAATAA ATATTTTCAT 
AATATGCAAT TCTGGCATGT CCTTGCTGCC AAGATGACCT TCATCATTGT TATGGAACAT 
GTTGTGTTTT TAGTTAAATT TTTGCTGGCC TGGATGATAC CTGATGTTCC AAAAGATGTT 
GTGGAGAGAA TCAAGAGAGA AAAGTTAATG ACTATCAAGA TTCTCCATGA TTTTGAGCTC 
AACAAATTAA AAGAGAACTT GGGAATTAAT TCTAATGAAT TTGCCAAGCA TGTCATGATT 
GAGGAAAACA AAGCACAGCT GGCTAAATCA ACACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136121.1
CDS	318..3056
Translation	MGDPDLLEVLAEEGEKVNKHIDYSFQMSESLSSRETSFLINEETMPAKRFNLFLRRRLMFQKNQQSKDSIFFRDGIRQIDFVLSYVDDVKKDAELKAERRKEFETNLRKTGLELEIEDKRDSEDGRTYFVKIHAPWEVLVTYAEVLGIKMPIKESDIPRPKHTPISYVLGPVRLPLSVKYPHPEYFTAQFSRHRQELFLIEDQATFFPSSSRNRIVYYILSRCPFGIEDGKKRFGIERLLNSNTYSSAYPLHDGQYWKPSEPPNPTNERYTLHQNWARFSYFYKEQPLDLIKNYYGEKIGIYFVFLGFYTEMLFFAAVVGLACFIYGLLSMEHNTSSTEICDPEIGGQMIMCPLCDQVCDYWRLNSTCLASKFSHLFDNESTVFFAIFMGIWVTLFLEFWKQRQARLEYEWDLVDFEEEQQQLQLRPEFEAMCKHRKLNAVTKEMEPYMPLYTRIPWYFLSGATVTLWMSLVVTSMVAVIVYRLSVFATFASFMESDASLKQVKSFLTPQITTSLTGSCLNFIVILILNFFYEKISAWITKMEIPRTYQEYESSLTLKMFLFQFVNFYSSCFYVAFFKGKFVGYPGKYTYLFNEWRSEECDPGGCLIELTTQLTIIMTGKQIFGNIKEAIYPLALNWWRRRKARTNSEKLYSRWEQDHDLESFGPLGLFYEYLETVTQFGFVTLFVASFPLAPLLALINNIVEIRVDAWKLTTQYRRTVASKAHSIGVWQDILYGMAVLSVATNAFIVAFTSDIIPRLVYYYAYSTNATQPMTGYVNNSLSVFLIADFPNHTAPSEKRDFITCRYRDYRYPPDDENKYFHNMQFWHVLAAKMTFIIVMEHVVFLVKFLLAWMIPDVPKDVVERIKREKLMTIKILHDFELNKLKENLGINSNEFAKHVMIEENKAQLAKSTL

Target ORF information:

RefSeq Version	NM_001142649.1
Organism	Homo sapiens(human)
Definition	Homo sapiens anoctamin 5 (ANO5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142649.1

ORF Insert Sequence:

ATGGGCGACC CGGATCTCCT GGAAGTGTTG GCGGAGGAAG GGGAAAAAGT CAATAAGCAT 
ATAGACTACT CTTTCCAAAT GAGTGAGAGC CTGAGCAGCA GAGAGACCAG CTTTCTCATC 
AATGAAGAAA CAATGCCTGC AAAGCGATTC AATTTGTTCC TGAGGCGGCG GCTTATGTTT 
CAAAAAAATC AGCAAAGCAA AGATTCTATC TTCTTCCGAG ATGGGATTAG GCAAATTGAT 
TTTGTGCTTT CCTACGTTGA TGATGTAAAG AAAGACGCAG AGTTAAAGGC GGAAAGAAGA 
AAAGAGTTTG AAACTAATCT CAGAAAAACA GGTCTTGAGT TGGAAATAGA AGACAAAAGG 
GACTCGGAAG ATGGAAGAAC TTATTTTGTC AAGATCCATG CCCCTTGGGA GGTATTAGTT 
ACCTATGCTG AAGTCTTGGG AATCAAAATG CCTATTAAGG AGAGTGATAT TCCCCGCCCT 
AAGCACACTC CTATAAGCTA TGTGCTTGGA CCTGTAAGAC TCCCACTGAG TGTGAAGTAT 
CCCCATCCTG AATATTTTAC TGCACAATTC AGCAGACATC GGCAGGAGCT CTTCCTCATC 
GAAGATCAGG CAACCTTCTT TCCATCCTCA TCAAGAAACA GAATTGTGTA CTATATTCTC 
TCAAGATGTC CTTTTGGCAT AGAAGATGGG AAGAAAAGGT TTGGGATTGA AAGACTGCTA 
AACTCTAACA CTTACTCATC TGCCTATCCA CTCCATGATG GCCAATATTG GAAGCCATCA 
GAACCTCCCA ATCCTACCAA TGAAAGATAC ACACTTCACC AGAATTGGGC TCGATTTTCC 
TATTTCTACA AGGAGCAGCC TTTAGACTTG ATTAAGAATT ATTATGGAGA AAAAATTGGT 
ATCTATTTTG TCTTTCTTGG ATTTTACACA GAAATGCTAT TCTTTGCAGC TGTAGTTGGC 
TTAGCTTGTT TTATTTATGG CTTATTATCA ATGGAACATA ACACAAGCAG CACTGAAATC 
TGTGACCCTG AGATTGGTGG TCAGATGATC ATGTGCCCAC TCTGTGATCA AGTGTGTGAT 
TATTGGAGAC TAAATAGTAC GTGTTTGGCT TCAAAGTTCT CCCATTTGTT TGATAATGAG 
TCAACAGTGT TCTTTGCAAT ATTCATGGGA ATTTGGGTCA CCTTATTTTT GGAGTTTTGG 
AAACAACGAC AAGCCAGACT GGAATATGAA TGGGACCTGG TGGACTTTGA AGAGGAACAG 
CAGCAGCTTC AGCTGAGACC AGAATTTGAA GCTATGTGTA AACACAGGAA ATTGAATGCA 
GTGACTAAGG AGATGGAACC TTACATGCCT CTATACACGC GTATTCCATG GTACTTTCTT 
TCAGGAGCCA CAGTGACATT ATGGATGTCT CTTGTCGTCA CCAGTATGGT AGCTGTAATT 
GTGTACCGCC TGTCAGTCTT TGCTACATTT GCTAGTTTCA TGGAAAGTGA TGCATCCTTA 
AAGCAGGTCA AAAGCTTCCT TACTCCTCAG ATAACCACAT CACTCACAGG ATCATGCTTG 
AACTTTATTG TCATCTTGAT CTTGAATTTC TTTTATGAAA AGATATCTGC CTGGATCACA 
AAAATGGAAA TTCCTCGAAC ATACCAGGAG TATGAGAGCA GTCTTACCTT GAAAATGTTC 
CTGTTTCAGT TTGTAAATTT TTACTCATCC TGCTTCTACG TAGCTTTCTT TAAAGGGAAG 
TTCGTAGGCT ATCCTGGAAA ATACACATAT TTATTTAATG AGTGGAGAAG TGAAGAGTGT 
GATCCTGGAG GCTGTCTTAT AGAATTGACA ACCCAATTGA CCATTATAAT GACCGGGAAA 
CAGATTTTTG GAAACATTAA AGAAGCCATT TATCCCTTGG CTTTGAATTG GTGGAGACGC 
CGAAAAGCTC GGACAAACTC TGAGAAGCTG TATAGTCGAT GGGAGCAGGA TCATGACCTT 
GAAAGTTTTG GACCCCTTGG GCTTTTCTAT GAGTACTTAG AAACAGTTAC TCAATTTGGA 
TTTGTTACAC TATTTGTGGC CTCTTTTCCT TTGGCTCCTC TTCTTGCTCT CATAAATAAT 
ATTGTAGAGA TTCGAGTGGA TGCCTGGAAA CTTACCACTC AATACAGGAG AACTGTAGCT 
TCTAAAGCTC ATAGCATAGG TGTTTGGCAA GACATTCTTT ATGGAATGGC TGTCCTTTCT 
GTTGCAACTA ATGCCTTTAT TGTTGCATTT ACGTCAGACA TCATTCCCCG TCTAGTTTAC 
TACTATGCTT ACTCAACAAA TGCCACACAG CCTATGACAG GATATGTGAA TAATAGCCTG 
TCAGTATTCC TGATAGCTGA TTTTCCAAAC CACACTGCAC CTTCGGAAAA ACGAGACTTC 
ATCACTTGCA GGTACAGAGA TTACAGATAT CCTCCTGATG ACGAGAATAA ATATTTTCAT 
AATATGCAAT TCTGGCATGT CCTTGCTGCC AAGATGACCT TCATCATTGT TATGGAACAT 
GTTGTGTTTT TAGTTAAATT TTTGCTGGCC TGGATGATAC CTGATGTTCC AAAAGATGTT 
GTGGAGAGAA TCAAGAGAGA AAAGTTAATG ACTATCAAGA TTCTCCATGA TTTTGAGCTC 
AACAAATTAA AAGAGAACTT GGGAATTAAT TCTAATGAAT TTGCCAAGCA TGTCATGATT 
GAGGAAAACA AAGCACAGCT GGCTAAATCA ACACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANO5 ( NM_001142649.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001136121.1 Homo sapiens anoctamin 5 (ANO5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26420
Clone ID Related Accession (Same CDS sequence)	NM_001142649.1 , NM_001142649.2
Accession Version	NM_001142649.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	anoctamin-5 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107886.2, AL832374.1, AB125267.1, AK125046.1, AC104009.7, AL833271.1 and BU742779.1. On Jun 2, 2019 this sequence version replaced NM_001142649.1. Transcript Variant: This variant (2) lacks an alternate in-frame segment, compared to variant 1, resulting in a shorter protein (isoform b), compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL832374.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGACC CGGATCTCCT GGAAGTGTTG GCGGAGGAAG GGGAAAAAGT CAATAAGCAT 
ATAGACTACT CTTTCCAAAT GAGTGAGAGC CTGAGCAGCA GAGAGACCAG CTTTCTCATC 
AATGAAGAAA CAATGCCTGC AAAGCGATTC AATTTGTTCC TGAGGCGGCG GCTTATGTTT 
CAAAAAAATC AGCAAAGCAA AGATTCTATC TTCTTCCGAG ATGGGATTAG GCAAATTGAT 
TTTGTGCTTT CCTACGTTGA TGATGTAAAG AAAGACGCAG AGTTAAAGGC GGAAAGAAGA 
AAAGAGTTTG AAACTAATCT CAGAAAAACA GGTCTTGAGT TGGAAATAGA AGACAAAAGG 
GACTCGGAAG ATGGAAGAAC TTATTTTGTC AAGATCCATG CCCCTTGGGA GGTATTAGTT 
ACCTATGCTG AAGTCTTGGG AATCAAAATG CCTATTAAGG AGAGTGATAT TCCCCGCCCT 
AAGCACACTC CTATAAGCTA TGTGCTTGGA CCTGTAAGAC TCCCACTGAG TGTGAAGTAT 
CCCCATCCTG AATATTTTAC TGCACAATTC AGCAGACATC GGCAGGAGCT CTTCCTCATC 
GAAGATCAGG CAACCTTCTT TCCATCCTCA TCAAGAAACA GAATTGTGTA CTATATTCTC 
TCAAGATGTC CTTTTGGCAT AGAAGATGGG AAGAAAAGGT TTGGGATTGA AAGACTGCTA 
AACTCTAACA CTTACTCATC TGCCTATCCA CTCCATGATG GCCAATATTG GAAGCCATCA 
GAACCTCCCA ATCCTACCAA TGAAAGATAC ACACTTCACC AGAATTGGGC TCGATTTTCC 
TATTTCTACA AGGAGCAGCC TTTAGACTTG ATTAAGAATT ATTATGGAGA AAAAATTGGT 
ATCTATTTTG TCTTTCTTGG ATTTTACACA GAAATGCTAT TCTTTGCAGC TGTAGTTGGC 
TTAGCTTGTT TTATTTATGG CTTATTATCA ATGGAACATA ACACAAGCAG CACTGAAATC 
TGTGACCCTG AGATTGGTGG TCAGATGATC ATGTGCCCAC TCTGTGATCA AGTGTGTGAT 
TATTGGAGAC TAAATAGTAC GTGTTTGGCT TCAAAGTTCT CCCATTTGTT TGATAATGAG 
TCAACAGTGT TCTTTGCAAT ATTCATGGGA ATTTGGGTCA CCTTATTTTT GGAGTTTTGG 
AAACAACGAC AAGCCAGACT GGAATATGAA TGGGACCTGG TGGACTTTGA AGAGGAACAG 
CAGCAGCTTC AGCTGAGACC AGAATTTGAA GCTATGTGTA AACACAGGAA ATTGAATGCA 
GTGACTAAGG AGATGGAACC TTACATGCCT CTATACACGC GTATTCCATG GTACTTTCTT 
TCAGGAGCCA CAGTGACATT ATGGATGTCT CTTGTCGTCA CCAGTATGGT AGCTGTAATT 
GTGTACCGCC TGTCAGTCTT TGCTACATTT GCTAGTTTCA TGGAAAGTGA TGCATCCTTA 
AAGCAGGTCA AAAGCTTCCT TACTCCTCAG ATAACCACAT CACTCACAGG ATCATGCTTG 
AACTTTATTG TCATCTTGAT CTTGAATTTC TTTTATGAAA AGATATCTGC CTGGATCACA 
AAAATGGAAA TTCCTCGAAC ATACCAGGAG TATGAGAGCA GTCTTACCTT GAAAATGTTC 
CTGTTTCAGT TTGTAAATTT TTACTCATCC TGCTTCTACG TAGCTTTCTT TAAAGGGAAG 
TTCGTAGGCT ATCCTGGAAA ATACACATAT TTATTTAATG AGTGGAGAAG TGAAGAGTGT 
GATCCTGGAG GCTGTCTTAT AGAATTGACA ACCCAATTGA CCATTATAAT GACCGGGAAA 
CAGATTTTTG GAAACATTAA AGAAGCCATT TATCCCTTGG CTTTGAATTG GTGGAGACGC 
CGAAAAGCTC GGACAAACTC TGAGAAGCTG TATAGTCGAT GGGAGCAGGA TCATGACCTT 
GAAAGTTTTG GACCCCTTGG GCTTTTCTAT GAGTACTTAG AAACAGTTAC TCAATTTGGA 
TTTGTTACAC TATTTGTGGC CTCTTTTCCT TTGGCTCCTC TTCTTGCTCT CATAAATAAT 
ATTGTAGAGA TTCGAGTGGA TGCCTGGAAA CTTACCACTC AATACAGGAG AACTGTAGCT 
TCTAAAGCTC ATAGCATAGG TGTTTGGCAA GACATTCTTT ATGGAATGGC TGTCCTTTCT 
GTTGCAACTA ATGCCTTTAT TGTTGCATTT ACGTCAGACA TCATTCCCCG TCTAGTTTAC 
TACTATGCTT ACTCAACAAA TGCCACACAG CCTATGACAG GATATGTGAA TAATAGCCTG 
TCAGTATTCC TGATAGCTGA TTTTCCAAAC CACACTGCAC CTTCGGAAAA ACGAGACTTC 
ATCACTTGCA GGTACAGAGA TTACAGATAT CCTCCTGATG ACGAGAATAA ATATTTTCAT 
AATATGCAAT TCTGGCATGT CCTTGCTGCC AAGATGACCT TCATCATTGT TATGGAACAT 
GTTGTGTTTT TAGTTAAATT TTTGCTGGCC TGGATGATAC CTGATGTTCC AAAAGATGTT 
GTGGAGAGAA TCAAGAGAGA AAAGTTAATG ACTATCAAGA TTCTCCATGA TTTTGAGCTC 
AACAAATTAA AAGAGAACTT GGGAATTAAT TCTAATGAAT TTGCCAAGCA TGTCATGATT 
GAGGAAAACA AAGCACAGCT GGCTAAATCA ACACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136121.1
CDS	409..3147
Translation	MGDPDLLEVLAEEGEKVNKHIDYSFQMSESLSSRETSFLINEETMPAKRFNLFLRRRLMFQKNQQSKDSIFFRDGIRQIDFVLSYVDDVKKDAELKAERRKEFETNLRKTGLELEIEDKRDSEDGRTYFVKIHAPWEVLVTYAEVLGIKMPIKESDIPRPKHTPISYVLGPVRLPLSVKYPHPEYFTAQFSRHRQELFLIEDQATFFPSSSRNRIVYYILSRCPFGIEDGKKRFGIERLLNSNTYSSAYPLHDGQYWKPSEPPNPTNERYTLHQNWARFSYFYKEQPLDLIKNYYGEKIGIYFVFLGFYTEMLFFAAVVGLACFIYGLLSMEHNTSSTEICDPEIGGQMIMCPLCDQVCDYWRLNSTCLASKFSHLFDNESTVFFAIFMGIWVTLFLEFWKQRQARLEYEWDLVDFEEEQQQLQLRPEFEAMCKHRKLNAVTKEMEPYMPLYTRIPWYFLSGATVTLWMSLVVTSMVAVIVYRLSVFATFASFMESDASLKQVKSFLTPQITTSLTGSCLNFIVILILNFFYEKISAWITKMEIPRTYQEYESSLTLKMFLFQFVNFYSSCFYVAFFKGKFVGYPGKYTYLFNEWRSEECDPGGCLIELTTQLTIIMTGKQIFGNIKEAIYPLALNWWRRRKARTNSEKLYSRWEQDHDLESFGPLGLFYEYLETVTQFGFVTLFVASFPLAPLLALINNIVEIRVDAWKLTTQYRRTVASKAHSIGVWQDILYGMAVLSVATNAFIVAFTSDIIPRLVYYYAYSTNATQPMTGYVNNSLSVFLIADFPNHTAPSEKRDFITCRYRDYRYPPDDENKYFHNMQFWHVLAAKMTFIIVMEHVVFLVKFLLAWMIPDVPKDVVERIKREKLMTIKILHDFELNKLKENLGINSNEFAKHVMIEENKAQLAKSTL

Target ORF information:

RefSeq Version	NM_001142649.2
Organism	Homo sapiens(human)
Definition	Homo sapiens anoctamin 5 (ANO5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142649.2

ORF Insert Sequence:

ATGGGCGACC CGGATCTCCT GGAAGTGTTG GCGGAGGAAG GGGAAAAAGT CAATAAGCAT 
ATAGACTACT CTTTCCAAAT GAGTGAGAGC CTGAGCAGCA GAGAGACCAG CTTTCTCATC 
AATGAAGAAA CAATGCCTGC AAAGCGATTC AATTTGTTCC TGAGGCGGCG GCTTATGTTT 
CAAAAAAATC AGCAAAGCAA AGATTCTATC TTCTTCCGAG ATGGGATTAG GCAAATTGAT 
TTTGTGCTTT CCTACGTTGA TGATGTAAAG AAAGACGCAG AGTTAAAGGC GGAAAGAAGA 
AAAGAGTTTG AAACTAATCT CAGAAAAACA GGTCTTGAGT TGGAAATAGA AGACAAAAGG 
GACTCGGAAG ATGGAAGAAC TTATTTTGTC AAGATCCATG CCCCTTGGGA GGTATTAGTT 
ACCTATGCTG AAGTCTTGGG AATCAAAATG CCTATTAAGG AGAGTGATAT TCCCCGCCCT 
AAGCACACTC CTATAAGCTA TGTGCTTGGA CCTGTAAGAC TCCCACTGAG TGTGAAGTAT 
CCCCATCCTG AATATTTTAC TGCACAATTC AGCAGACATC GGCAGGAGCT CTTCCTCATC 
GAAGATCAGG CAACCTTCTT TCCATCCTCA TCAAGAAACA GAATTGTGTA CTATATTCTC 
TCAAGATGTC CTTTTGGCAT AGAAGATGGG AAGAAAAGGT TTGGGATTGA AAGACTGCTA 
AACTCTAACA CTTACTCATC TGCCTATCCA CTCCATGATG GCCAATATTG GAAGCCATCA 
GAACCTCCCA ATCCTACCAA TGAAAGATAC ACACTTCACC AGAATTGGGC TCGATTTTCC 
TATTTCTACA AGGAGCAGCC TTTAGACTTG ATTAAGAATT ATTATGGAGA AAAAATTGGT 
ATCTATTTTG TCTTTCTTGG ATTTTACACA GAAATGCTAT TCTTTGCAGC TGTAGTTGGC 
TTAGCTTGTT TTATTTATGG CTTATTATCA ATGGAACATA ACACAAGCAG CACTGAAATC 
TGTGACCCTG AGATTGGTGG TCAGATGATC ATGTGCCCAC TCTGTGATCA AGTGTGTGAT 
TATTGGAGAC TAAATAGTAC GTGTTTGGCT TCAAAGTTCT CCCATTTGTT TGATAATGAG 
TCAACAGTGT TCTTTGCAAT ATTCATGGGA ATTTGGGTCA CCTTATTTTT GGAGTTTTGG 
AAACAACGAC AAGCCAGACT GGAATATGAA TGGGACCTGG TGGACTTTGA AGAGGAACAG 
CAGCAGCTTC AGCTGAGACC AGAATTTGAA GCTATGTGTA AACACAGGAA ATTGAATGCA 
GTGACTAAGG AGATGGAACC TTACATGCCT CTATACACGC GTATTCCATG GTACTTTCTT 
TCAGGAGCCA CAGTGACATT ATGGATGTCT CTTGTCGTCA CCAGTATGGT AGCTGTAATT 
GTGTACCGCC TGTCAGTCTT TGCTACATTT GCTAGTTTCA TGGAAAGTGA TGCATCCTTA 
AAGCAGGTCA AAAGCTTCCT TACTCCTCAG ATAACCACAT CACTCACAGG ATCATGCTTG 
AACTTTATTG TCATCTTGAT CTTGAATTTC TTTTATGAAA AGATATCTGC CTGGATCACA 
AAAATGGAAA TTCCTCGAAC ATACCAGGAG TATGAGAGCA GTCTTACCTT GAAAATGTTC 
CTGTTTCAGT TTGTAAATTT TTACTCATCC TGCTTCTACG TAGCTTTCTT TAAAGGGAAG 
TTCGTAGGCT ATCCTGGAAA ATACACATAT TTATTTAATG AGTGGAGAAG TGAAGAGTGT 
GATCCTGGAG GCTGTCTTAT AGAATTGACA ACCCAATTGA CCATTATAAT GACCGGGAAA 
CAGATTTTTG GAAACATTAA AGAAGCCATT TATCCCTTGG CTTTGAATTG GTGGAGACGC 
CGAAAAGCTC GGACAAACTC TGAGAAGCTG TATAGTCGAT GGGAGCAGGA TCATGACCTT 
GAAAGTTTTG GACCCCTTGG GCTTTTCTAT GAGTACTTAG AAACAGTTAC TCAATTTGGA 
TTTGTTACAC TATTTGTGGC CTCTTTTCCT TTGGCTCCTC TTCTTGCTCT CATAAATAAT 
ATTGTAGAGA TTCGAGTGGA TGCCTGGAAA CTTACCACTC AATACAGGAG AACTGTAGCT 
TCTAAAGCTC ATAGCATAGG TGTTTGGCAA GACATTCTTT ATGGAATGGC TGTCCTTTCT 
GTTGCAACTA ATGCCTTTAT TGTTGCATTT ACGTCAGACA TCATTCCCCG TCTAGTTTAC 
TACTATGCTT ACTCAACAAA TGCCACACAG CCTATGACAG GATATGTGAA TAATAGCCTG 
TCAGTATTCC TGATAGCTGA TTTTCCAAAC CACACTGCAC CTTCGGAAAA ACGAGACTTC 
ATCACTTGCA GGTACAGAGA TTACAGATAT CCTCCTGATG ACGAGAATAA ATATTTTCAT 
AATATGCAAT TCTGGCATGT CCTTGCTGCC AAGATGACCT TCATCATTGT TATGGAACAT 
GTTGTGTTTT TAGTTAAATT TTTGCTGGCC TGGATGATAC CTGATGTTCC AAAAGATGTT 
GTGGAGAGAA TCAAGAGAGA AAAGTTAATG ACTATCAAGA TTCTCCATGA TTTTGAGCTC 
AACAAATTAA AAGAGAACTT GGGAATTAAT TCTAATGAAT TTGCCAAGCA TGTCATGATT 
GAGGAAAACA AAGCACAGCT GGCTAAATCA ACACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANO5 ( XM_005252822.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005252879.1 Homo sapiens anoctamin 5 (ANO5), transcript variant X4, mRNA.

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CloneID	OHu44795
Clone ID Related Accession (Same CDS sequence)	XM_005252822.4
Accession Version	XM_005252822.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	anoctamin-5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005252822.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGC AGAGCCTGAG CAGCAGAGAG ACCAGCTTTC TCATCAATGA AGAAACAATG 
CCTGCAAAGC GATTCAATTT GTTCCTGAGG CGGCGGCTTA TGTTTCAAAA AAATCAGCAA 
AGCAAAGATT CTATCTTCTT CCGAGATGGG ATTAGGCAAA TTGATTTTGT GCTTTCCTAC 
GTTGATGATG TAAAGAAAGA CGCAGAGTTA AAGGCGGAAA GAAGAAAAGA GTTTGAAACT 
AATCTCAGAA AAACAGGTCT TGAGTTGGAA ATAGAAGACA AAAGGGACTC GGAAGATGGA 
AGAACTTATT TTGTCAAGAT CCATGCCCCT TGGGAGGTAT TAGTTACCTA TGCTGAAGTC 
TTGGGAATCA AAATGCCTAT TAAGGAGAGT GATATTCCCC GCCCTAAGCA CACTCCTATA 
AGCTATGTGC TTGGACCTGT AAGACTCCCA CTGAGTGTGA AGTATCCCCA TCCTGAATAT 
TTTACTGCAC AATTCAGCAG ACATCGGCAG GAGCTCTTCC TCATCGAAGA TCAGGCAACC 
TTCTTTCCAT CCTCATCAAG AAACAGAATT GTGTACTATA TTCTCTCAAG ATGTCCTTTT 
GGCATAGAAG ATGGGAAGAA AAGGTTTGGG ATTGAAAGAC TGCTAAACTC TAACACTTAC 
TCATCTGCCT ATCCACTCCA TGATGGCCAA TATTGGAAGC CATCAGAACC TCCCAATCCT 
ACCAATGAAA GATACACACT TCACCAGAAT TGGGCTCGAT TTTCCTATTT CTACAAGGAG 
CAGCCTTTAG ACTTGATTAA GAATTATTAT GGAGAAAAAA TTGGTATCTA TTTTGTCTTT 
CTTGGATTTT ACACAGAAAT GCTATTCTTT GCAGCTGTAG TTGGCTTAGC TTGTTTTATT 
TATGGCTTAT TATCAATGGA ACATAACACA AGCAGCACTG AAATCTGTGA CCCTGAGATT 
GGTGGTCAGA TGATCATGTG CCCACTCTGT GATCAAGTGT GTGATTATTG GAGACTAAAT 
AGTACGTGTT TGGCTTCAAA GTTCTCCCAT TTGTTTGATA ATGAGTCAAC AGTGTTCTTT 
GCAATATTCA TGGGAATTTG GGTCACCTTA TTTTTGGAGT TTTGGAAACA ACGACAAGCC 
AGACTGGAAT ATGAATGGGA CCTGGTGGAC TTTGAAGAGG AACAGCAGCA GCTTCAGCTG 
AGACCAGAAT TTGAAGCTAT GTGTAAACAC AGGAAATTGA ATGCAGTGAC TAAGGAGATG 
GAACCTTACA TGCCTCTATA CACGCGTATT CCATGGTACT TTCTTTCAGG AGCCACAGTG 
ACATTATGGA TGTCTCTTGT CGTCACCAGT ATGGTAGCTG TAATTGTGTA CCGCCTGTCA 
GTCTTTGCTA CATTTGCTAG TTTCATGGAA AGTGATGCAT CCTTAAAGCA GGTCAAAAGC 
TTCCTTACTC CTCAGATAAC CACATCACTC ACAGGATCAT GCTTGAACTT TATTGTCATC 
TTGATCTTGA ATTTCTTTTA TGAAAAGATA TCTGCCTGGA TCACAAAAAT GGAAATTCCT 
CGAACATACC AGGAGTATGA GAGCAGTCTT ACCTTGAAAA TGTTCCTGTT TCAGTTTGTA 
AATTTTTACT CATCCTGCTT CTACGTAGCT TTCTTTAAAG GGAAGTTCGT AGGCTATCCT 
GGAAAATACA CATATTTATT TAATGAGTGG AGAAGTGAAG AGTGTGATCC TGGAGGCTGT 
CTTATAGAAT TGACAACCCA ATTGACCATT ATAATGACCG GGAAACAGAT TTTTGGAAAC 
ATTAAAGAAG CCATTTATCC CTTGGCTTTG AATTGGTGGA GACGCCGAAA AGCTCGGACA 
AACTCTGAGA AGCTGTATAG TCGATGGGAG CAGGATCATG ACCTTGAAAG TTTTGGACCC 
CTTGGGCTTT TCTATGAGTA CTTAGAAACA GTTACTCAAT TTGGATTTGT TACACTATTT 
GTGGCCTCTT TTCCTTTGGC TCCTCTTCTT GCTCTCATAA ATAATATTGT AGAGATTCGA 
GTGGATGCCT GGAAACTTAC CACTCAATAC AGGAGAACTG TAGCTTCTAA AGCTCATAGC 
ATAGGTGTTT GGCAAGACAT TCTTTATGGA ATGGCTGTCC TTTCTGTTGC AACTAATGCC 
TTTATTGTTG CATTTACGTC AGACATCATT CCCCGTCTAG TTTACTACTA TGCTTACTCA 
ACAAATGCCA CACAGCCTAT GACAGGATAT GTGAATAATA GCCTGTCAGT ATTCCTGATA 
GCTGATTTTC CAAACCACAC TGCACCTTCG GAAAAACGAG ACTTCATCAC TTGCAGGTAC 
AGAGATTACA GATATCCTCC TGATGACGAG AATAAATATT TTCATAATAT GCAATTCTGG 
CATGTCCTTG CTGCCAAGAT GACCTTCATC ATTGTTATGG AACATGTTGT GTTTTTAGTT 
AAATTTTTGC TGGCCTGGAT GATACCTGAT GTTCCAAAAG ATGTTGTGGA GAGAATCAAG 
AGAGAAAAGT TAATGACTAT CAAGATTCTC CATGATTTTG AGCTCAACAA ATTAAAAGAG 
AACTTGGGAA TTAATTCTAA TGAATTTGCC AAGCATGTCA TGATTGAGGA AAACAAAGCA 
CAGCTGGCTA AATCAACACT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005252879.1
CDS	281..2944
Translation	MSEQSLSSRETSFLINEETMPAKRFNLFLRRRLMFQKNQQSKDSIFFRDGIRQIDFVLSYVDDVKKDAELKAERRKEFETNLRKTGLELEIEDKRDSEDGRTYFVKIHAPWEVLVTYAEVLGIKMPIKESDIPRPKHTPISYVLGPVRLPLSVKYPHPEYFTAQFSRHRQELFLIEDQATFFPSSSRNRIVYYILSRCPFGIEDGKKRFGIERLLNSNTYSSAYPLHDGQYWKPSEPPNPTNERYTLHQNWARFSYFYKEQPLDLIKNYYGEKIGIYFVFLGFYTEMLFFAAVVGLACFIYGLLSMEHNTSSTEICDPEIGGQMIMCPLCDQVCDYWRLNSTCLASKFSHLFDNESTVFFAIFMGIWVTLFLEFWKQRQARLEYEWDLVDFEEEQQQLQLRPEFEAMCKHRKLNAVTKEMEPYMPLYTRIPWYFLSGATVTLWMSLVVTSMVAVIVYRLSVFATFASFMESDASLKQVKSFLTPQITTSLTGSCLNFIVILILNFFYEKISAWITKMEIPRTYQEYESSLTLKMFLFQFVNFYSSCFYVAFFKGKFVGYPGKYTYLFNEWRSEECDPGGCLIELTTQLTIIMTGKQIFGNIKEAIYPLALNWWRRRKARTNSEKLYSRWEQDHDLESFGPLGLFYEYLETVTQFGFVTLFVASFPLAPLLALINNIVEIRVDAWKLTTQYRRTVASKAHSIGVWQDILYGMAVLSVATNAFIVAFTSDIIPRLVYYYAYSTNATQPMTGYVNNSLSVFLIADFPNHTAPSEKRDFITCRYRDYRYPPDDENKYFHNMQFWHVLAAKMTFIIVMEHVVFLVKFLLAWMIPDVPKDVVERIKREKLMTIKILHDFELNKLKENLGINSNEFAKHVMIEENKAQLAKSTL

Target ORF information:

RefSeq Version	XM_005252822.4
Organism	Homo sapiens(human)
Definition	Homo sapiens anoctamin 5 (ANO5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005252822.4

ORF Insert Sequence:

ATGAGTGAGC AGAGCCTGAG CAGCAGAGAG ACCAGCTTTC TCATCAATGA AGAAACAATG 
CCTGCAAAGC GATTCAATTT GTTCCTGAGG CGGCGGCTTA TGTTTCAAAA AAATCAGCAA 
AGCAAAGATT CTATCTTCTT CCGAGATGGG ATTAGGCAAA TTGATTTTGT GCTTTCCTAC 
GTTGATGATG TAAAGAAAGA CGCAGAGTTA AAGGCGGAAA GAAGAAAAGA GTTTGAAACT 
AATCTCAGAA AAACAGGTCT TGAGTTGGAA ATAGAAGACA AAAGGGACTC GGAAGATGGA 
AGAACTTATT TTGTCAAGAT CCATGCCCCT TGGGAGGTAT TAGTTACCTA TGCTGAAGTC 
TTGGGAATCA AAATGCCTAT TAAGGAGAGT GATATTCCCC GCCCTAAGCA CACTCCTATA 
AGCTATGTGC TTGGACCTGT AAGACTCCCA CTGAGTGTGA AGTATCCCCA TCCTGAATAT 
TTTACTGCAC AATTCAGCAG ACATCGGCAG GAGCTCTTCC TCATCGAAGA TCAGGCAACC 
TTCTTTCCAT CCTCATCAAG AAACAGAATT GTGTACTATA TTCTCTCAAG ATGTCCTTTT 
GGCATAGAAG ATGGGAAGAA AAGGTTTGGG ATTGAAAGAC TGCTAAACTC TAACACTTAC 
TCATCTGCCT ATCCACTCCA TGATGGCCAA TATTGGAAGC CATCAGAACC TCCCAATCCT 
ACCAATGAAA GATACACACT TCACCAGAAT TGGGCTCGAT TTTCCTATTT CTACAAGGAG 
CAGCCTTTAG ACTTGATTAA GAATTATTAT GGAGAAAAAA TTGGTATCTA TTTTGTCTTT 
CTTGGATTTT ACACAGAAAT GCTATTCTTT GCAGCTGTAG TTGGCTTAGC TTGTTTTATT 
TATGGCTTAT TATCAATGGA ACATAACACA AGCAGCACTG AAATCTGTGA CCCTGAGATT 
GGTGGTCAGA TGATCATGTG CCCACTCTGT GATCAAGTGT GTGATTATTG GAGACTAAAT 
AGTACGTGTT TGGCTTCAAA GTTCTCCCAT TTGTTTGATA ATGAGTCAAC AGTGTTCTTT 
GCAATATTCA TGGGAATTTG GGTCACCTTA TTTTTGGAGT TTTGGAAACA ACGACAAGCC 
AGACTGGAAT ATGAATGGGA CCTGGTGGAC TTTGAAGAGG AACAGCAGCA GCTTCAGCTG 
AGACCAGAAT TTGAAGCTAT GTGTAAACAC AGGAAATTGA ATGCAGTGAC TAAGGAGATG 
GAACCTTACA TGCCTCTATA CACGCGTATT CCATGGTACT TTCTTTCAGG AGCCACAGTG 
ACATTATGGA TGTCTCTTGT CGTCACCAGT ATGGTAGCTG TAATTGTGTA CCGCCTGTCA 
GTCTTTGCTA CATTTGCTAG TTTCATGGAA AGTGATGCAT CCTTAAAGCA GGTCAAAAGC 
TTCCTTACTC CTCAGATAAC CACATCACTC ACAGGATCAT GCTTGAACTT TATTGTCATC 
TTGATCTTGA ATTTCTTTTA TGAAAAGATA TCTGCCTGGA TCACAAAAAT GGAAATTCCT 
CGAACATACC AGGAGTATGA GAGCAGTCTT ACCTTGAAAA TGTTCCTGTT TCAGTTTGTA 
AATTTTTACT CATCCTGCTT CTACGTAGCT TTCTTTAAAG GGAAGTTCGT AGGCTATCCT 
GGAAAATACA CATATTTATT TAATGAGTGG AGAAGTGAAG AGTGTGATCC TGGAGGCTGT 
CTTATAGAAT TGACAACCCA ATTGACCATT ATAATGACCG GGAAACAGAT TTTTGGAAAC 
ATTAAAGAAG CCATTTATCC CTTGGCTTTG AATTGGTGGA GACGCCGAAA AGCTCGGACA 
AACTCTGAGA AGCTGTATAG TCGATGGGAG CAGGATCATG ACCTTGAAAG TTTTGGACCC 
CTTGGGCTTT TCTATGAGTA CTTAGAAACA GTTACTCAAT TTGGATTTGT TACACTATTT 
GTGGCCTCTT TTCCTTTGGC TCCTCTTCTT GCTCTCATAA ATAATATTGT AGAGATTCGA 
GTGGATGCCT GGAAACTTAC CACTCAATAC AGGAGAACTG TAGCTTCTAA AGCTCATAGC 
ATAGGTGTTT GGCAAGACAT TCTTTATGGA ATGGCTGTCC TTTCTGTTGC AACTAATGCC 
TTTATTGTTG CATTTACGTC AGACATCATT CCCCGTCTAG TTTACTACTA TGCTTACTCA 
ACAAATGCCA CACAGCCTAT GACAGGATAT GTGAATAATA GCCTGTCAGT ATTCCTGATA 
GCTGATTTTC CAAACCACAC TGCACCTTCG GAAAAACGAG ACTTCATCAC TTGCAGGTAC 
AGAGATTACA GATATCCTCC TGATGACGAG AATAAATATT TTCATAATAT GCAATTCTGG 
CATGTCCTTG CTGCCAAGAT GACCTTCATC ATTGTTATGG AACATGTTGT GTTTTTAGTT 
AAATTTTTGC TGGCCTGGAT GATACCTGAT GTTCCAAAAG ATGTTGTGGA GAGAATCAAG 
AGAGAAAAGT TAATGACTAT CAAGATTCTC CATGATTTTG AGCTCAACAA ATTAAAAGAG 
AACTTGGGAA TTAATTCTAA TGAATTTGCC AAGCATGTCA TGATTGAGGA AAACAAAGCA 
CAGCTGGCTA AATCAACACT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANO5 ( XM_005252820.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005252877.2 Homo sapiens anoctamin 5 (ANO5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu77856
Clone ID Related Accession (Same CDS sequence)	XM_005252820.3
Accession Version	XM_005252820.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	anoctamin-5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005252820.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CGGATCTCCT GGAAGTGTTG GCGGAGGAAG GGGAAAAAGT CAATAAGCAT 
ATAGACTACT CTTTCCAAAT GAGTGAGCAG AGCCTGAGCA GCAGAGAGAC CAGCTTTCTC 
ATCAATGAAG AAACAATGTT TCAAAAAAAT CAGCAAAGCA AAGATTCTAT CTTCTTCCGA 
GATGGGATTA GGCAAATTGA TTTTGTGCTT TCCTACGTTG ATGATGTAAA GAAAGACGCA 
GAGTTAAAGG CGGAAAGAAG AAAAGAGTTT GAAACTAATC TCAGAAAAAC AGGTCTTGAG 
TTGGAAATAG AAGACAAAAG GGACTCGGAA GATGGAAGAA CTTATTTTGT CAAGATCCAT 
GCCCCTTGGG AGGTATTAGT TACCTATGCT GAAGTCTTGG GAATCAAAAT GCCTATTAAG 
GAGAGTGATA TTCCCCGCCC TAAGCACACT CCTATAAGCT ATGTGCTTGG ACCTGTAAGA 
CTCCCACTGA GTGTGAAGTA TCCCCATCCT GAATATTTTA CTGCACAATT CAGCAGACAT 
CGGCAGGAGC TCTTCCTCAT CGAAGATCAG GCAACCTTCT TTCCATCCTC ATCAAGAAAC 
AGAATTGTGT ACTATATTCT CTCAAGATGT CCTTTTGGCA TAGAAGATGG GAAGAAAAGG 
TTTGGGATTG AAAGACTGCT AAACTCTAAC ACTTACTCAT CTGCCTATCC ACTCCATGAT 
GGCCAATATT GGAAGCCATC AGAACCTCCC AATCCTACCA ATGAAAGATA CACACTTCAC 
CAGAATTGGG CTCGATTTTC CTATTTCTAC AAGGAGCAGC CTTTAGACTT GATTAAGAAT 
TATTATGGAG AAAAAATTGG TATCTATTTT GTCTTTCTTG GATTTTACAC AGAAATGCTA 
TTCTTTGCAG CTGTAGTTGG CTTAGCTTGT TTTATTTATG GCTTATTATC AATGGAACAT 
AACACAAGCA GCACTGAAAT CTGTGACCCT GAGATTGGTG GTCAGATGAT CATGTGCCCA 
CTCTGTGATC AAGTGTGTGA TTATTGGAGA CTAAATAGTA CGTGTTTGGC TTCAAAGTTC 
TCCCATTTGT TTGATAATGA GTCAACAGTG TTCTTTGCAA TATTCATGGG AATTTGGGTC 
ACCTTATTTT TGGAGTTTTG GAAACAACGA CAAGCCAGAC TGGAATATGA ATGGGACCTG 
GTGGACTTTG AAGAGGAACA GCAGCAGCTT CAGCTGAGAC CAGAATTTGA AGCTATGTGT 
AAACACAGGA AATTGAATGC AGTGACTAAG GAGATGGAAC CTTACATGCC TCTATACACG 
CGTATTCCAT GGTACTTTCT TTCAGGAGCC ACAGTGACAT TATGGATGTC TCTTGTCGTC 
ACCAGTATGG TAGCTGTAAT TGTGTACCGC CTGTCAGTCT TTGCTACATT TGCTAGTTTC 
ATGGAAAGTG ATGCATCCTT AAAGCAGGTC AAAAGCTTCC TTACTCCTCA GATAACCACA 
TCACTCACAG GATCATGCTT GAACTTTATT GTCATCTTGA TCTTGAATTT CTTTTATGAA 
AAGATATCTG CCTGGATCAC AAAAATGGAA ATTCCTCGAA CATACCAGGA GTATGAGAGC 
AGTCTTACCT TGAAAATGTT CCTGTTTCAG TTTGTAAATT TTTACTCATC CTGCTTCTAC 
GTAGCTTTCT TTAAAGGGAA GTTCGTAGGC TATCCTGGAA AATACACATA TTTATTTAAT 
GAGTGGAGAA GTGAAGAGTG TGATCCTGGA GGCTGTCTTA TAGAATTGAC AACCCAATTG 
ACCATTATAA TGACCGGGAA ACAGATTTTT GGAAACATTA AAGAAGCCAT TTATCCCTTG 
GCTTTGAATT GGTGGAGACG CCGAAAAGCT CGGACAAACT CTGAGAAGCT GTATAGTCGA 
TGGGAGCAGG ATCATGACCT TGAAAGTTTT GGACCCCTTG GGCTTTTCTA TGAGTACTTA 
GAAACAGTTA CTCAATTTGG ATTTGTTACA CTATTTGTGG CCTCTTTTCC TTTGGCTCCT 
CTTCTTGCTC TCATAAATAA TATTGTAGAG ATTCGAGTGG ATGCCTGGAA ACTTACCACT 
CAATACAGGA GAACTGTAGC TTCTAAAGCT CATAGCATAG GTGTTTGGCA AGACATTCTT 
TATGGAATGG CTGTCCTTTC TGTTGCAACT AATGCCTTTA TTGTTGCATT TACGTCAGAC 
ATCATTCCCC GTCTAGTTTA CTACTATGCT TACTCAACAA ATGCCACACA GCCTATGACA 
GGATATGTGA ATAATAGCCT GTCAGTATTC CTGATAGCTG ATTTTCCAAA CCACACTGCA 
CCTTCGGAAA AACGAGACTT CATCACTTGC AGGTACAGAG ATTACAGATA TCCTCCTGAT 
GACGAGAATA AATATTTTCA TAATATGCAA TTCTGGCATG TCCTTGCTGC CAAGATGACC 
TTCATCATTG TTATGGAACA TGTTGTGTTT TTAGTTAAAT TTTTGCTGGC CTGGATGATA 
CCTGATGTTC CAAAAGATGT TGTGGAGAGA ATCAAGAGAG AAAAGTTAAT GACTATCAAG 
ATTCTCCATG ATTTTGAGCT CAACAAATTA AAAGAGAACT TGGGAATTAA TTCTAATGAA 
TTTGCCAAGC ATGTCATGAT TGAGGAAAAC AAAGCACAGC TGGCTAAATC AACACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005252877.2
CDS	464..3163
Translation	MGDPDLLEVLAEEGEKVNKHIDYSFQMSEQSLSSRETSFLINEETMFQKNQQSKDSIFFRDGIRQIDFVLSYVDDVKKDAELKAERRKEFETNLRKTGLELEIEDKRDSEDGRTYFVKIHAPWEVLVTYAEVLGIKMPIKESDIPRPKHTPISYVLGPVRLPLSVKYPHPEYFTAQFSRHRQELFLIEDQATFFPSSSRNRIVYYILSRCPFGIEDGKKRFGIERLLNSNTYSSAYPLHDGQYWKPSEPPNPTNERYTLHQNWARFSYFYKEQPLDLIKNYYGEKIGIYFVFLGFYTEMLFFAAVVGLACFIYGLLSMEHNTSSTEICDPEIGGQMIMCPLCDQVCDYWRLNSTCLASKFSHLFDNESTVFFAIFMGIWVTLFLEFWKQRQARLEYEWDLVDFEEEQQQLQLRPEFEAMCKHRKLNAVTKEMEPYMPLYTRIPWYFLSGATVTLWMSLVVTSMVAVIVYRLSVFATFASFMESDASLKQVKSFLTPQITTSLTGSCLNFIVILILNFFYEKISAWITKMEIPRTYQEYESSLTLKMFLFQFVNFYSSCFYVAFFKGKFVGYPGKYTYLFNEWRSEECDPGGCLIELTTQLTIIMTGKQIFGNIKEAIYPLALNWWRRRKARTNSEKLYSRWEQDHDLESFGPLGLFYEYLETVTQFGFVTLFVASFPLAPLLALINNIVEIRVDAWKLTTQYRRTVASKAHSIGVWQDILYGMAVLSVATNAFIVAFTSDIIPRLVYYYAYSTNATQPMTGYVNNSLSVFLIADFPNHTAPSEKRDFITCRYRDYRYPPDDENKYFHNMQFWHVLAAKMTFIIVMEHVVFLVKFLLAWMIPDVPKDVVERIKREKLMTIKILHDFELNKLKENLGINSNEFAKHVMIEENKAQLAKSTL

Target ORF information:

RefSeq Version	XM_005252820.3
Organism	Homo sapiens(human)
Definition	Homo sapiens anoctamin 5 (ANO5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005252820.3

ORF Insert Sequence:

ATGGGCGACC CGGATCTCCT GGAAGTGTTG GCGGAGGAAG GGGAAAAAGT CAATAAGCAT 
ATAGACTACT CTTTCCAAAT GAGTGAGCAG AGCCTGAGCA GCAGAGAGAC CAGCTTTCTC 
ATCAATGAAG AAACAATGTT TCAAAAAAAT CAGCAAAGCA AAGATTCTAT CTTCTTCCGA 
GATGGGATTA GGCAAATTGA TTTTGTGCTT TCCTACGTTG ATGATGTAAA GAAAGACGCA 
GAGTTAAAGG CGGAAAGAAG AAAAGAGTTT GAAACTAATC TCAGAAAAAC AGGTCTTGAG 
TTGGAAATAG AAGACAAAAG GGACTCGGAA GATGGAAGAA CTTATTTTGT CAAGATCCAT 
GCCCCTTGGG AGGTATTAGT TACCTATGCT GAAGTCTTGG GAATCAAAAT GCCTATTAAG 
GAGAGTGATA TTCCCCGCCC TAAGCACACT CCTATAAGCT ATGTGCTTGG ACCTGTAAGA 
CTCCCACTGA GTGTGAAGTA TCCCCATCCT GAATATTTTA CTGCACAATT CAGCAGACAT 
CGGCAGGAGC TCTTCCTCAT CGAAGATCAG GCAACCTTCT TTCCATCCTC ATCAAGAAAC 
AGAATTGTGT ACTATATTCT CTCAAGATGT CCTTTTGGCA TAGAAGATGG GAAGAAAAGG 
TTTGGGATTG AAAGACTGCT AAACTCTAAC ACTTACTCAT CTGCCTATCC ACTCCATGAT 
GGCCAATATT GGAAGCCATC AGAACCTCCC AATCCTACCA ATGAAAGATA CACACTTCAC 
CAGAATTGGG CTCGATTTTC CTATTTCTAC AAGGAGCAGC CTTTAGACTT GATTAAGAAT 
TATTATGGAG AAAAAATTGG TATCTATTTT GTCTTTCTTG GATTTTACAC AGAAATGCTA 
TTCTTTGCAG CTGTAGTTGG CTTAGCTTGT TTTATTTATG GCTTATTATC AATGGAACAT 
AACACAAGCA GCACTGAAAT CTGTGACCCT GAGATTGGTG GTCAGATGAT CATGTGCCCA 
CTCTGTGATC AAGTGTGTGA TTATTGGAGA CTAAATAGTA CGTGTTTGGC TTCAAAGTTC 
TCCCATTTGT TTGATAATGA GTCAACAGTG TTCTTTGCAA TATTCATGGG AATTTGGGTC 
ACCTTATTTT TGGAGTTTTG GAAACAACGA CAAGCCAGAC TGGAATATGA ATGGGACCTG 
GTGGACTTTG AAGAGGAACA GCAGCAGCTT CAGCTGAGAC CAGAATTTGA AGCTATGTGT 
AAACACAGGA AATTGAATGC AGTGACTAAG GAGATGGAAC CTTACATGCC TCTATACACG 
CGTATTCCAT GGTACTTTCT TTCAGGAGCC ACAGTGACAT TATGGATGTC TCTTGTCGTC 
ACCAGTATGG TAGCTGTAAT TGTGTACCGC CTGTCAGTCT TTGCTACATT TGCTAGTTTC 
ATGGAAAGTG ATGCATCCTT AAAGCAGGTC AAAAGCTTCC TTACTCCTCA GATAACCACA 
TCACTCACAG GATCATGCTT GAACTTTATT GTCATCTTGA TCTTGAATTT CTTTTATGAA 
AAGATATCTG CCTGGATCAC AAAAATGGAA ATTCCTCGAA CATACCAGGA GTATGAGAGC 
AGTCTTACCT TGAAAATGTT CCTGTTTCAG TTTGTAAATT TTTACTCATC CTGCTTCTAC 
GTAGCTTTCT TTAAAGGGAA GTTCGTAGGC TATCCTGGAA AATACACATA TTTATTTAAT 
GAGTGGAGAA GTGAAGAGTG TGATCCTGGA GGCTGTCTTA TAGAATTGAC AACCCAATTG 
ACCATTATAA TGACCGGGAA ACAGATTTTT GGAAACATTA AAGAAGCCAT TTATCCCTTG 
GCTTTGAATT GGTGGAGACG CCGAAAAGCT CGGACAAACT CTGAGAAGCT GTATAGTCGA 
TGGGAGCAGG ATCATGACCT TGAAAGTTTT GGACCCCTTG GGCTTTTCTA TGAGTACTTA 
GAAACAGTTA CTCAATTTGG ATTTGTTACA CTATTTGTGG CCTCTTTTCC TTTGGCTCCT 
CTTCTTGCTC TCATAAATAA TATTGTAGAG ATTCGAGTGG ATGCCTGGAA ACTTACCACT 
CAATACAGGA GAACTGTAGC TTCTAAAGCT CATAGCATAG GTGTTTGGCA AGACATTCTT 
TATGGAATGG CTGTCCTTTC TGTTGCAACT AATGCCTTTA TTGTTGCATT TACGTCAGAC 
ATCATTCCCC GTCTAGTTTA CTACTATGCT TACTCAACAA ATGCCACACA GCCTATGACA 
GGATATGTGA ATAATAGCCT GTCAGTATTC CTGATAGCTG ATTTTCCAAA CCACACTGCA 
CCTTCGGAAA AACGAGACTT CATCACTTGC AGGTACAGAG ATTACAGATA TCCTCCTGAT 
GACGAGAATA AATATTTTCA TAATATGCAA TTCTGGCATG TCCTTGCTGC CAAGATGACC 
TTCATCATTG TTATGGAACA TGTTGTGTTT TTAGTTAAAT TTTTGCTGGC CTGGATGATA 
CCTGATGTTC CAAAAGATGT TGTGGAGAGA ATCAAGAGAG AAAAGTTAAT GACTATCAAG 
ATTCTCCATG ATTTTGAGCT CAACAAATTA AAAGAGAACT TGGGAATTAA TTCTAATGAA 
TTTGCCAAGC ATGTCATGAT TGAGGAAAAC AAAGCACAGC TGGCTAAATC AACACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANO5 ( XM_005252821.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005252878.2 Homo sapiens anoctamin 5 (ANO5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu77857
Clone ID Related Accession (Same CDS sequence)	XM_005252821.3
Accession Version	XM_005252821.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	anoctamin-5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005252821.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CGGATCTCCT GGAAGTGTTG GCGGAGGAAG GGGAAAAAGT CAATAAGCAT 
ATAGACTACT CTTTCCAAAT GAGTGAGAGC CTGAGCAGCA GAGAGACCAG CTTTCTCATC 
AATGAAGAAA CAATGTTTCA AAAAAATCAG CAAAGCAAAG ATTCTATCTT CTTCCGAGAT 
GGGATTAGGC AAATTGATTT TGTGCTTTCC TACGTTGATG ATGTAAAGAA AGACGCAGAG 
TTAAAGGCGG AAAGAAGAAA AGAGTTTGAA ACTAATCTCA GAAAAACAGG TCTTGAGTTG 
GAAATAGAAG ACAAAAGGGA CTCGGAAGAT GGAAGAACTT ATTTTGTCAA GATCCATGCC 
CCTTGGGAGG TATTAGTTAC CTATGCTGAA GTCTTGGGAA TCAAAATGCC TATTAAGGAG 
AGTGATATTC CCCGCCCTAA GCACACTCCT ATAAGCTATG TGCTTGGACC TGTAAGACTC 
CCACTGAGTG TGAAGTATCC CCATCCTGAA TATTTTACTG CACAATTCAG CAGACATCGG 
CAGGAGCTCT TCCTCATCGA AGATCAGGCA ACCTTCTTTC CATCCTCATC AAGAAACAGA 
ATTGTGTACT ATATTCTCTC AAGATGTCCT TTTGGCATAG AAGATGGGAA GAAAAGGTTT 
GGGATTGAAA GACTGCTAAA CTCTAACACT TACTCATCTG CCTATCCACT CCATGATGGC 
CAATATTGGA AGCCATCAGA ACCTCCCAAT CCTACCAATG AAAGATACAC ACTTCACCAG 
AATTGGGCTC GATTTTCCTA TTTCTACAAG GAGCAGCCTT TAGACTTGAT TAAGAATTAT 
TATGGAGAAA AAATTGGTAT CTATTTTGTC TTTCTTGGAT TTTACACAGA AATGCTATTC 
TTTGCAGCTG TAGTTGGCTT AGCTTGTTTT ATTTATGGCT TATTATCAAT GGAACATAAC 
ACAAGCAGCA CTGAAATCTG TGACCCTGAG ATTGGTGGTC AGATGATCAT GTGCCCACTC 
TGTGATCAAG TGTGTGATTA TTGGAGACTA AATAGTACGT GTTTGGCTTC AAAGTTCTCC 
CATTTGTTTG ATAATGAGTC AACAGTGTTC TTTGCAATAT TCATGGGAAT TTGGGTCACC 
TTATTTTTGG AGTTTTGGAA ACAACGACAA GCCAGACTGG AATATGAATG GGACCTGGTG 
GACTTTGAAG AGGAACAGCA GCAGCTTCAG CTGAGACCAG AATTTGAAGC TATGTGTAAA 
CACAGGAAAT TGAATGCAGT GACTAAGGAG ATGGAACCTT ACATGCCTCT ATACACGCGT 
ATTCCATGGT ACTTTCTTTC AGGAGCCACA GTGACATTAT GGATGTCTCT TGTCGTCACC 
AGTATGGTAG CTGTAATTGT GTACCGCCTG TCAGTCTTTG CTACATTTGC TAGTTTCATG 
GAAAGTGATG CATCCTTAAA GCAGGTCAAA AGCTTCCTTA CTCCTCAGAT AACCACATCA 
CTCACAGGAT CATGCTTGAA CTTTATTGTC ATCTTGATCT TGAATTTCTT TTATGAAAAG 
ATATCTGCCT GGATCACAAA AATGGAAATT CCTCGAACAT ACCAGGAGTA TGAGAGCAGT 
CTTACCTTGA AAATGTTCCT GTTTCAGTTT GTAAATTTTT ACTCATCCTG CTTCTACGTA 
GCTTTCTTTA AAGGGAAGTT CGTAGGCTAT CCTGGAAAAT ACACATATTT ATTTAATGAG 
TGGAGAAGTG AAGAGTGTGA TCCTGGAGGC TGTCTTATAG AATTGACAAC CCAATTGACC 
ATTATAATGA CCGGGAAACA GATTTTTGGA AACATTAAAG AAGCCATTTA TCCCTTGGCT 
TTGAATTGGT GGAGACGCCG AAAAGCTCGG ACAAACTCTG AGAAGCTGTA TAGTCGATGG 
GAGCAGGATC ATGACCTTGA AAGTTTTGGA CCCCTTGGGC TTTTCTATGA GTACTTAGAA 
ACAGTTACTC AATTTGGATT TGTTACACTA TTTGTGGCCT CTTTTCCTTT GGCTCCTCTT 
CTTGCTCTCA TAAATAATAT TGTAGAGATT CGAGTGGATG CCTGGAAACT TACCACTCAA 
TACAGGAGAA CTGTAGCTTC TAAAGCTCAT AGCATAGGTG TTTGGCAAGA CATTCTTTAT 
GGAATGGCTG TCCTTTCTGT TGCAACTAAT GCCTTTATTG TTGCATTTAC GTCAGACATC 
ATTCCCCGTC TAGTTTACTA CTATGCTTAC TCAACAAATG CCACACAGCC TATGACAGGA 
TATGTGAATA ATAGCCTGTC AGTATTCCTG ATAGCTGATT TTCCAAACCA CACTGCACCT 
TCGGAAAAAC GAGACTTCAT CACTTGCAGG TACAGAGATT ACAGATATCC TCCTGATGAC 
GAGAATAAAT ATTTTCATAA TATGCAATTC TGGCATGTCC TTGCTGCCAA GATGACCTTC 
ATCATTGTTA TGGAACATGT TGTGTTTTTA GTTAAATTTT TGCTGGCCTG GATGATACCT 
GATGTTCCAA AAGATGTTGT GGAGAGAATC AAGAGAGAAA AGTTAATGAC TATCAAGATT 
CTCCATGATT TTGAGCTCAA CAAATTAAAA GAGAACTTGG GAATTAATTC TAATGAATTT 
GCCAAGCATG TCATGATTGA GGAAAACAAA GCACAGCTGG CTAAATCAAC ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005252878.2
CDS	464..3160
Translation	MGDPDLLEVLAEEGEKVNKHIDYSFQMSESLSSRETSFLINEETMFQKNQQSKDSIFFRDGIRQIDFVLSYVDDVKKDAELKAERRKEFETNLRKTGLELEIEDKRDSEDGRTYFVKIHAPWEVLVTYAEVLGIKMPIKESDIPRPKHTPISYVLGPVRLPLSVKYPHPEYFTAQFSRHRQELFLIEDQATFFPSSSRNRIVYYILSRCPFGIEDGKKRFGIERLLNSNTYSSAYPLHDGQYWKPSEPPNPTNERYTLHQNWARFSYFYKEQPLDLIKNYYGEKIGIYFVFLGFYTEMLFFAAVVGLACFIYGLLSMEHNTSSTEICDPEIGGQMIMCPLCDQVCDYWRLNSTCLASKFSHLFDNESTVFFAIFMGIWVTLFLEFWKQRQARLEYEWDLVDFEEEQQQLQLRPEFEAMCKHRKLNAVTKEMEPYMPLYTRIPWYFLSGATVTLWMSLVVTSMVAVIVYRLSVFATFASFMESDASLKQVKSFLTPQITTSLTGSCLNFIVILILNFFYEKISAWITKMEIPRTYQEYESSLTLKMFLFQFVNFYSSCFYVAFFKGKFVGYPGKYTYLFNEWRSEECDPGGCLIELTTQLTIIMTGKQIFGNIKEAIYPLALNWWRRRKARTNSEKLYSRWEQDHDLESFGPLGLFYEYLETVTQFGFVTLFVASFPLAPLLALINNIVEIRVDAWKLTTQYRRTVASKAHSIGVWQDILYGMAVLSVATNAFIVAFTSDIIPRLVYYYAYSTNATQPMTGYVNNSLSVFLIADFPNHTAPSEKRDFITCRYRDYRYPPDDENKYFHNMQFWHVLAAKMTFIIVMEHVVFLVKFLLAWMIPDVPKDVVERIKREKLMTIKILHDFELNKLKENLGINSNEFAKHVMIEENKAQLAKSTL

Target ORF information:

RefSeq Version	XM_005252821.3
Organism	Homo sapiens(human)
Definition	Homo sapiens anoctamin 5 (ANO5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005252821.3

ORF Insert Sequence:

ATGGGCGACC CGGATCTCCT GGAAGTGTTG GCGGAGGAAG GGGAAAAAGT CAATAAGCAT 
ATAGACTACT CTTTCCAAAT GAGTGAGAGC CTGAGCAGCA GAGAGACCAG CTTTCTCATC 
AATGAAGAAA CAATGTTTCA AAAAAATCAG CAAAGCAAAG ATTCTATCTT CTTCCGAGAT 
GGGATTAGGC AAATTGATTT TGTGCTTTCC TACGTTGATG ATGTAAAGAA AGACGCAGAG 
TTAAAGGCGG AAAGAAGAAA AGAGTTTGAA ACTAATCTCA GAAAAACAGG TCTTGAGTTG 
GAAATAGAAG ACAAAAGGGA CTCGGAAGAT GGAAGAACTT ATTTTGTCAA GATCCATGCC 
CCTTGGGAGG TATTAGTTAC CTATGCTGAA GTCTTGGGAA TCAAAATGCC TATTAAGGAG 
AGTGATATTC CCCGCCCTAA GCACACTCCT ATAAGCTATG TGCTTGGACC TGTAAGACTC 
CCACTGAGTG TGAAGTATCC CCATCCTGAA TATTTTACTG CACAATTCAG CAGACATCGG 
CAGGAGCTCT TCCTCATCGA AGATCAGGCA ACCTTCTTTC CATCCTCATC AAGAAACAGA 
ATTGTGTACT ATATTCTCTC AAGATGTCCT TTTGGCATAG AAGATGGGAA GAAAAGGTTT 
GGGATTGAAA GACTGCTAAA CTCTAACACT TACTCATCTG CCTATCCACT CCATGATGGC 
CAATATTGGA AGCCATCAGA ACCTCCCAAT CCTACCAATG AAAGATACAC ACTTCACCAG 
AATTGGGCTC GATTTTCCTA TTTCTACAAG GAGCAGCCTT TAGACTTGAT TAAGAATTAT 
TATGGAGAAA AAATTGGTAT CTATTTTGTC TTTCTTGGAT TTTACACAGA AATGCTATTC 
TTTGCAGCTG TAGTTGGCTT AGCTTGTTTT ATTTATGGCT TATTATCAAT GGAACATAAC 
ACAAGCAGCA CTGAAATCTG TGACCCTGAG ATTGGTGGTC AGATGATCAT GTGCCCACTC 
TGTGATCAAG TGTGTGATTA TTGGAGACTA AATAGTACGT GTTTGGCTTC AAAGTTCTCC 
CATTTGTTTG ATAATGAGTC AACAGTGTTC TTTGCAATAT TCATGGGAAT TTGGGTCACC 
TTATTTTTGG AGTTTTGGAA ACAACGACAA GCCAGACTGG AATATGAATG GGACCTGGTG 
GACTTTGAAG AGGAACAGCA GCAGCTTCAG CTGAGACCAG AATTTGAAGC TATGTGTAAA 
CACAGGAAAT TGAATGCAGT GACTAAGGAG ATGGAACCTT ACATGCCTCT ATACACGCGT 
ATTCCATGGT ACTTTCTTTC AGGAGCCACA GTGACATTAT GGATGTCTCT TGTCGTCACC 
AGTATGGTAG CTGTAATTGT GTACCGCCTG TCAGTCTTTG CTACATTTGC TAGTTTCATG 
GAAAGTGATG CATCCTTAAA GCAGGTCAAA AGCTTCCTTA CTCCTCAGAT AACCACATCA 
CTCACAGGAT CATGCTTGAA CTTTATTGTC ATCTTGATCT TGAATTTCTT TTATGAAAAG 
ATATCTGCCT GGATCACAAA AATGGAAATT CCTCGAACAT ACCAGGAGTA TGAGAGCAGT 
CTTACCTTGA AAATGTTCCT GTTTCAGTTT GTAAATTTTT ACTCATCCTG CTTCTACGTA 
GCTTTCTTTA AAGGGAAGTT CGTAGGCTAT CCTGGAAAAT ACACATATTT ATTTAATGAG 
TGGAGAAGTG AAGAGTGTGA TCCTGGAGGC TGTCTTATAG AATTGACAAC CCAATTGACC 
ATTATAATGA CCGGGAAACA GATTTTTGGA AACATTAAAG AAGCCATTTA TCCCTTGGCT 
TTGAATTGGT GGAGACGCCG AAAAGCTCGG ACAAACTCTG AGAAGCTGTA TAGTCGATGG 
GAGCAGGATC ATGACCTTGA AAGTTTTGGA CCCCTTGGGC TTTTCTATGA GTACTTAGAA 
ACAGTTACTC AATTTGGATT TGTTACACTA TTTGTGGCCT CTTTTCCTTT GGCTCCTCTT 
CTTGCTCTCA TAAATAATAT TGTAGAGATT CGAGTGGATG CCTGGAAACT TACCACTCAA 
TACAGGAGAA CTGTAGCTTC TAAAGCTCAT AGCATAGGTG TTTGGCAAGA CATTCTTTAT 
GGAATGGCTG TCCTTTCTGT TGCAACTAAT GCCTTTATTG TTGCATTTAC GTCAGACATC 
ATTCCCCGTC TAGTTTACTA CTATGCTTAC TCAACAAATG CCACACAGCC TATGACAGGA 
TATGTGAATA ATAGCCTGTC AGTATTCCTG ATAGCTGATT TTCCAAACCA CACTGCACCT 
TCGGAAAAAC GAGACTTCAT CACTTGCAGG TACAGAGATT ACAGATATCC TCCTGATGAC 
GAGAATAAAT ATTTTCATAA TATGCAATTC TGGCATGTCC TTGCTGCCAA GATGACCTTC 
ATCATTGTTA TGGAACATGT TGTGTTTTTA GTTAAATTTT TGCTGGCCTG GATGATACCT 
GATGTTCCAA AAGATGTTGT GGAGAGAATC AAGAGAGAAA AGTTAATGAC TATCAAGATT 
CTCCATGATT TTGAGCTCAA CAAATTAAAA GAGAACTTGG GAATTAATTC TAATGAATTT 
GCCAAGCATG TCATGATTGA GGAAAACAAA GCACAGCTGG CTAAATCAAC ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANO5 ( XM_011519949.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518251.1 Homo sapiens anoctamin 5 (ANO5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu77858
Clone ID Related Accession (Same CDS sequence)	XM_011519949.2
Accession Version	XM_011519949.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	anoctamin-5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519949.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CGGATCTCCT GGAAGTGTTG GCGGAGGAAG GGGAAAAAGT CAATAAGCAT 
ATAGACTACT CTTTCCAAAT GAGTGAGTTT CAAAAAAATC AGCAAAGCAA AGATTCTATC 
TTCTTCCGAG ATGGGATTAG GCAAATTGAT TTTGTGCTTT CCTACGTTGA TGATGTAAAG 
AAAGACGCAG AGTTAAAGGC GGAAAGAAGA AAAGAGTTTG AAACTAATCT CAGAAAAACA 
GGTCTTGAGT TGGAAATAGA AGACAAAAGG GACTCGGAAG ATGGAAGAAC TTATTTTGTC 
AAGATCCATG CCCCTTGGGA GGTATTAGTT ACCTATGCTG AAGTCTTGGG AATCAAAATG 
CCTATTAAGG AGAGTGATAT TCCCCGCCCT AAGCACACTC CTATAAGCTA TGTGCTTGGA 
CCTGTAAGAC TCCCACTGAG TGTGAAGTAT CCCCATCCTG AATATTTTAC TGCACAATTC 
AGCAGACATC GGCAGGAGCT CTTCCTCATC GAAGATCAGG CAACCTTCTT TCCATCCTCA 
TCAAGAAACA GAATTGTGTA CTATATTCTC TCAAGATGTC CTTTTGGCAT AGAAGATGGG 
AAGAAAAGGT TTGGGATTGA AAGACTGCTA AACTCTAACA CTTACTCATC TGCCTATCCA 
CTCCATGATG GCCAATATTG GAAGCCATCA GAACCTCCCA ATCCTACCAA TGAAAGATAC 
ACACTTCACC AGAATTGGGC TCGATTTTCC TATTTCTACA AGGAGCAGCC TTTAGACTTG 
ATTAAGAATT ATTATGGAGA AAAAATTGGT ATCTATTTTG TCTTTCTTGG ATTTTACACA 
GAAATGCTAT TCTTTGCAGC TGTAGTTGGC TTAGCTTGTT TTATTTATGG CTTATTATCA 
ATGGAACATA ACACAAGCAG CACTGAAATC TGTGACCCTG AGATTGGTGG TCAGATGATC 
ATGTGCCCAC TCTGTGATCA AGTGTGTGAT TATTGGAGAC TAAATAGTAC GTGTTTGGCT 
TCAAAGTTCT CCCATTTGTT TGATAATGAG TCAACAGTGT TCTTTGCAAT ATTCATGGGA 
ATTTGGGTCA CCTTATTTTT GGAGTTTTGG AAACAACGAC AAGCCAGACT GGAATATGAA 
TGGGACCTGG TGGACTTTGA AGAGGAACAG CAGCAGCTTC AGCTGAGACC AGAATTTGAA 
GCTATGTGTA AACACAGGAA ATTGAATGCA GTGACTAAGG AGATGGAACC TTACATGCCT 
CTATACACGC GTATTCCATG GTACTTTCTT TCAGGAGCCA CAGTGACATT ATGGATGTCT 
CTTGTCGTCA CCAGTATGGT AGCTGTAATT GTGTACCGCC TGTCAGTCTT TGCTACATTT 
GCTAGTTTCA TGGAAAGTGA TGCATCCTTA AAGCAGGTCA AAAGCTTCCT TACTCCTCAG 
ATAACCACAT CACTCACAGG ATCATGCTTG AACTTTATTG TCATCTTGAT CTTGAATTTC 
TTTTATGAAA AGATATCTGC CTGGATCACA AAAATGGAAA TTCCTCGAAC ATACCAGGAG 
TATGAGAGCA GTCTTACCTT GAAAATGTTC CTGTTTCAGT TTGTAAATTT TTACTCATCC 
TGCTTCTACG TAGCTTTCTT TAAAGGGAAG TTCGTAGGCT ATCCTGGAAA ATACACATAT 
TTATTTAATG AGTGGAGAAG TGAAGAGTGT GATCCTGGAG GCTGTCTTAT AGAATTGACA 
ACCCAATTGA CCATTATAAT GACCGGGAAA CAGATTTTTG GAAACATTAA AGAAGCCATT 
TATCCCTTGG CTTTGAATTG GTGGAGACGC CGAAAAGCTC GGACAAACTC TGAGAAGCTG 
TATAGTCGAT GGGAGCAGGA TCATGACCTT GAAAGTTTTG GACCCCTTGG GCTTTTCTAT 
GAGTACTTAG AAACAGTTAC TCAATTTGGA TTTGTTACAC TATTTGTGGC CTCTTTTCCT 
TTGGCTCCTC TTCTTGCTCT CATAAATAAT ATTGTAGAGA TTCGAGTGGA TGCCTGGAAA 
CTTACCACTC AATACAGGAG AACTGTAGCT TCTAAAGCTC ATAGCATAGG TGTTTGGCAA 
GACATTCTTT ATGGAATGGC TGTCCTTTCT GTTGCAACTA ATGCCTTTAT TGTTGCATTT 
ACGTCAGACA TCATTCCCCG TCTAGTTTAC TACTATGCTT ACTCAACAAA TGCCACACAG 
CCTATGACAG GATATGTGAA TAATAGCCTG TCAGTATTCC TGATAGCTGA TTTTCCAAAC 
CACACTGCAC CTTCGGAAAA ACGAGACTTC ATCACTTGCA GGTACAGAGA TTACAGATAT 
CCTCCTGATG ACGAGAATAA ATATTTTCAT AATATGCAAT TCTGGCATGT CCTTGCTGCC 
AAGATGACCT TCATCATTGT TATGGAACAT GTTGTGTTTT TAGTTAAATT TTTGCTGGCC 
TGGATGATAC CTGATGTTCC AAAAGATGTT GTGGAGAGAA TCAAGAGAGA AAAGTTAATG 
ACTATCAAGA TTCTCCATGA TTTTGAGCTC AACAAATTAA AAGAGAACTT GGGAATTAAT 
TCTAATGAAT TTGCCAAGCA TGTCATGATT GAGGAAAACA AAGCACAGCT GGCTAAATCA 
ACACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518251.1
CDS	464..3112
Translation	MGDPDLLEVLAEEGEKVNKHIDYSFQMSEFQKNQQSKDSIFFRDGIRQIDFVLSYVDDVKKDAELKAERRKEFETNLRKTGLELEIEDKRDSEDGRTYFVKIHAPWEVLVTYAEVLGIKMPIKESDIPRPKHTPISYVLGPVRLPLSVKYPHPEYFTAQFSRHRQELFLIEDQATFFPSSSRNRIVYYILSRCPFGIEDGKKRFGIERLLNSNTYSSAYPLHDGQYWKPSEPPNPTNERYTLHQNWARFSYFYKEQPLDLIKNYYGEKIGIYFVFLGFYTEMLFFAAVVGLACFIYGLLSMEHNTSSTEICDPEIGGQMIMCPLCDQVCDYWRLNSTCLASKFSHLFDNESTVFFAIFMGIWVTLFLEFWKQRQARLEYEWDLVDFEEEQQQLQLRPEFEAMCKHRKLNAVTKEMEPYMPLYTRIPWYFLSGATVTLWMSLVVTSMVAVIVYRLSVFATFASFMESDASLKQVKSFLTPQITTSLTGSCLNFIVILILNFFYEKISAWITKMEIPRTYQEYESSLTLKMFLFQFVNFYSSCFYVAFFKGKFVGYPGKYTYLFNEWRSEECDPGGCLIELTTQLTIIMTGKQIFGNIKEAIYPLALNWWRRRKARTNSEKLYSRWEQDHDLESFGPLGLFYEYLETVTQFGFVTLFVASFPLAPLLALINNIVEIRVDAWKLTTQYRRTVASKAHSIGVWQDILYGMAVLSVATNAFIVAFTSDIIPRLVYYYAYSTNATQPMTGYVNNSLSVFLIADFPNHTAPSEKRDFITCRYRDYRYPPDDENKYFHNMQFWHVLAAKMTFIIVMEHVVFLVKFLLAWMIPDVPKDVVERIKREKLMTIKILHDFELNKLKENLGINSNEFAKHVMIEENKAQLAKSTL

Target ORF information:

RefSeq Version	XM_011519949.2
Organism	Homo sapiens(human)
Definition	Homo sapiens anoctamin 5 (ANO5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519949.2

ORF Insert Sequence:

ATGGGCGACC CGGATCTCCT GGAAGTGTTG GCGGAGGAAG GGGAAAAAGT CAATAAGCAT 
ATAGACTACT CTTTCCAAAT GAGTGAGTTT CAAAAAAATC AGCAAAGCAA AGATTCTATC 
TTCTTCCGAG ATGGGATTAG GCAAATTGAT TTTGTGCTTT CCTACGTTGA TGATGTAAAG 
AAAGACGCAG AGTTAAAGGC GGAAAGAAGA AAAGAGTTTG AAACTAATCT CAGAAAAACA 
GGTCTTGAGT TGGAAATAGA AGACAAAAGG GACTCGGAAG ATGGAAGAAC TTATTTTGTC 
AAGATCCATG CCCCTTGGGA GGTATTAGTT ACCTATGCTG AAGTCTTGGG AATCAAAATG 
CCTATTAAGG AGAGTGATAT TCCCCGCCCT AAGCACACTC CTATAAGCTA TGTGCTTGGA 
CCTGTAAGAC TCCCACTGAG TGTGAAGTAT CCCCATCCTG AATATTTTAC TGCACAATTC 
AGCAGACATC GGCAGGAGCT CTTCCTCATC GAAGATCAGG CAACCTTCTT TCCATCCTCA 
TCAAGAAACA GAATTGTGTA CTATATTCTC TCAAGATGTC CTTTTGGCAT AGAAGATGGG 
AAGAAAAGGT TTGGGATTGA AAGACTGCTA AACTCTAACA CTTACTCATC TGCCTATCCA 
CTCCATGATG GCCAATATTG GAAGCCATCA GAACCTCCCA ATCCTACCAA TGAAAGATAC 
ACACTTCACC AGAATTGGGC TCGATTTTCC TATTTCTACA AGGAGCAGCC TTTAGACTTG 
ATTAAGAATT ATTATGGAGA AAAAATTGGT ATCTATTTTG TCTTTCTTGG ATTTTACACA 
GAAATGCTAT TCTTTGCAGC TGTAGTTGGC TTAGCTTGTT TTATTTATGG CTTATTATCA 
ATGGAACATA ACACAAGCAG CACTGAAATC TGTGACCCTG AGATTGGTGG TCAGATGATC 
ATGTGCCCAC TCTGTGATCA AGTGTGTGAT TATTGGAGAC TAAATAGTAC GTGTTTGGCT 
TCAAAGTTCT CCCATTTGTT TGATAATGAG TCAACAGTGT TCTTTGCAAT ATTCATGGGA 
ATTTGGGTCA CCTTATTTTT GGAGTTTTGG AAACAACGAC AAGCCAGACT GGAATATGAA 
TGGGACCTGG TGGACTTTGA AGAGGAACAG CAGCAGCTTC AGCTGAGACC AGAATTTGAA 
GCTATGTGTA AACACAGGAA ATTGAATGCA GTGACTAAGG AGATGGAACC TTACATGCCT 
CTATACACGC GTATTCCATG GTACTTTCTT TCAGGAGCCA CAGTGACATT ATGGATGTCT 
CTTGTCGTCA CCAGTATGGT AGCTGTAATT GTGTACCGCC TGTCAGTCTT TGCTACATTT 
GCTAGTTTCA TGGAAAGTGA TGCATCCTTA AAGCAGGTCA AAAGCTTCCT TACTCCTCAG 
ATAACCACAT CACTCACAGG ATCATGCTTG AACTTTATTG TCATCTTGAT CTTGAATTTC 
TTTTATGAAA AGATATCTGC CTGGATCACA AAAATGGAAA TTCCTCGAAC ATACCAGGAG 
TATGAGAGCA GTCTTACCTT GAAAATGTTC CTGTTTCAGT TTGTAAATTT TTACTCATCC 
TGCTTCTACG TAGCTTTCTT TAAAGGGAAG TTCGTAGGCT ATCCTGGAAA ATACACATAT 
TTATTTAATG AGTGGAGAAG TGAAGAGTGT GATCCTGGAG GCTGTCTTAT AGAATTGACA 
ACCCAATTGA CCATTATAAT GACCGGGAAA CAGATTTTTG GAAACATTAA AGAAGCCATT 
TATCCCTTGG CTTTGAATTG GTGGAGACGC CGAAAAGCTC GGACAAACTC TGAGAAGCTG 
TATAGTCGAT GGGAGCAGGA TCATGACCTT GAAAGTTTTG GACCCCTTGG GCTTTTCTAT 
GAGTACTTAG AAACAGTTAC TCAATTTGGA TTTGTTACAC TATTTGTGGC CTCTTTTCCT 
TTGGCTCCTC TTCTTGCTCT CATAAATAAT ATTGTAGAGA TTCGAGTGGA TGCCTGGAAA 
CTTACCACTC AATACAGGAG AACTGTAGCT TCTAAAGCTC ATAGCATAGG TGTTTGGCAA 
GACATTCTTT ATGGAATGGC TGTCCTTTCT GTTGCAACTA ATGCCTTTAT TGTTGCATTT 
ACGTCAGACA TCATTCCCCG TCTAGTTTAC TACTATGCTT ACTCAACAAA TGCCACACAG 
CCTATGACAG GATATGTGAA TAATAGCCTG TCAGTATTCC TGATAGCTGA TTTTCCAAAC 
CACACTGCAC CTTCGGAAAA ACGAGACTTC ATCACTTGCA GGTACAGAGA TTACAGATAT 
CCTCCTGATG ACGAGAATAA ATATTTTCAT AATATGCAAT TCTGGCATGT CCTTGCTGCC 
AAGATGACCT TCATCATTGT TATGGAACAT GTTGTGTTTT TAGTTAAATT TTTGCTGGCC 
TGGATGATAC CTGATGTTCC AAAAGATGTT GTGGAGAGAA TCAAGAGAGA AAAGTTAATG 
ACTATCAAGA TTCTCCATGA TTTTGAGCTC AACAAATTAA AAGAGAACTT GGGAATTAAT 
TCTAATGAAT TTGCCAAGCA TGTCATGATT GAGGAAAACA AAGCACAGCT GGCTAAATCA 
ACACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gain of function of TMEM16E/ANO5 scrambling activity caused by a mutation associated with gnathodiaphyseal dysplasia.
Cellular and molecular life sciences : CMLS(2017 Nov)
Di Zanni E,Gradogna A,Scholz-Starke J,Boccaccio A

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ANO5 cDNA ORF clone, Homo sapiens(human)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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