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EFEMP1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol EFEMP1
Entrez Gene ID 2202
Full Name EGF containing fibulin like extracellular matrix protein 1
Synonyms DHRD,DRAD,FBLN3,FBNL,FIBL-3,MLVT,MTLV,S1-5
General protein information
Preferred Names

EGF containing fibulin like extracellular matrix protein 1

Names

EGF-containing fibulin-like extracellular matrix protein 1
extracellular protein S1-5
fibulin-3

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

2

2p16.1

Summary This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.[provided by RefSeq, Nov 2009].
Disorder MIM:

601548

Disorder Html: Doyne honeycomb degeneration of retina, 126600 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_001039348.2 NP_001034437.1 EGF-containing fibulin-like extracellular matrix protein 1 precursor
XM_005264205.4 XP_005264262.1 EGF-containing fibulin-like extracellular matrix protein 1 isoform X1
XM_017003586.1 XP_016859075.1 EGF-containing fibulin-like extracellular matrix protein 1 isoform X2
NM_001039349.2 NP_001034438.1 EGF-containing fibulin-like extracellular matrix protein 1 precursor



Canis lupus familiaris (dog) EFEMP1 XP_005626175.1
Gallus gallus (chicken) EFEMP1 XP_426097.3
Danio rerio (zebrafish) LOC100332567 XP_002664117.2
Mus musculus (house mouse) Efemp1 NP_666127.2
Homo sapiens (human) EFEMP1 NP_001034438.1
Bos taurus (cattle) EFEMP1 NP_001075186.1
Rattus norvegicus (Norway rat) Efemp1 NP_001012039.1
Xenopus tropicalis (tropical clawed frog) efemp1 XP_002936872.1


Related articles in PubMed

Comparison of the Diagnostic Performance of Fibulin-3 and Mesothelin in Patients with Pleural Effusions from Malignant Mesothelioma.
Battolla E, Canessa PA, Ferro P, Franceschini MC, Fontana V, Dessanti P, Pinelli V, Morabito A, Fedeli F, Pistillo MP, Roncella S
Anticancer research37(3)1387-1391(2017 03)

Assessing the association between EFEMP1 rs3791679 polymorphism and risk of glioma in a Chinese Han population.
Jiang N, Peng YP, Wang XY, Dou CW, He WM
Genetics and molecular research : GMR15(3)(2016 Aug)

EFEMP1 inhibits migration of hepatocellular carcinoma by regulating MMP2 and MMP9 via ERK1/2 activity.
Dou CY, Cao CJ, Wang Z, Zhang RH, Huang LL, Lian JY, Xie WL, Wang LT
Oncology reports35(6)3489-95(2016 Jun)

Transcriptional regulation and expression of the dominant drusen gene FBLN3 (EFEMP1) in mammalian retina.
Blackburn J, Tarttelin EE, Gregory-Evans CY, Moosajee M, Gregory-Evans K
Investigative ophthalmology & visual science44(11)4613-21(2003 Nov)

Aberrant accumulation of EFEMP1 underlies drusen formation in Malattia Leventinese and age-related macular degeneration.
Marmorstein LY, Munier FL, Arsenijevic Y, Schorderet DF, McLaughlin PJ, Chung D, Traboulsi E, Marmorstein AD
Proceedings of the National Academy of Sciences of the United States of America99(20)13067-72(2002 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SMRP but not FBLN3 has a role in pleural effusions in in malignant pleural mesothelioma
Title: Comparison of the Diagnostic Performance of Fibulin-3 and Mesothelin in Patients with Pleural Effusions from Malignant Mesothelioma.

Serum EFEMP1 is down-regulated and involved in the progression of Prostate Cancer. It may serve as a useful diagnostic biomarker, with better diagnostic accuracy than PSA in Prostate Cancer.
Title: Epidermal Growth Factor-Containing Fibulin-Like Extracellular Matrix Protein 1 (EFEMP1) Acts as a Potential Diagnostic Biomarker for Prostate Cancer.

The EFEMP1 rs3791679 polymorphism influences glioma susceptibility in the Chinese Han population.
Title: Assessing the association between EFEMP1 rs3791679 polymorphism and risk of glioma in a Chinese Han population.

Extensive methylation of the EFEMP1 gene promoter can discriminate invasive from benign IPMNs with superior accuracy owing to their stepwise accumulation of tumor progression
Title: Expansion of epigenetic alterations in EFEMP1 promoter predicts malignant formation in pancreatobiliary intraductal papillary mucinous neoplasms.

EFEMP1 negatively modulates the migration ability in hepatocellular carcinoma. Reduction of EFEMP1 promotes the migration ability though increasing Matrix Metalloproteinase 2 and Matrix Metalloproteinase 9 expression via Extracellular Signal-Regulated MAP Kinase 1/2 activity.
Title: EFEMP1 inhibits migration of hepatocellular carcinoma by regulating MMP2 and MMP9 via ERK1/2 activity.

The following EFEMP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EFEMP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu22367 NM_001039348.2
Latest version!
Homo sapiens EGF containing fibulin like extracellular matrix protein 1 (EFEMP1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu22367 NM_001039349.2
Latest version!
Homo sapiens EGF containing fibulin like extracellular matrix protein 1 (EFEMP1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu89157 XM_017003586.1
Latest version!
Homo sapiens EGF containing fibulin like extracellular matrix protein 1 (EFEMP1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
OHu35325 XM_005264205.4
Latest version!
Homo sapiens EGF containing fibulin like extracellular matrix protein 1 (EFEMP1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22367
Clone ID Related Accession (Same CDS sequence) NM_001039348.2 , NM_001039349.2
Accession Version NM_001039348.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product EGF-containing fibulin-like extracellular matrix protein 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA264704.1, AK312639.1, AK290599.1, AC010895.8 and U03877.1. This sequence is a reference standard in the RefSeqGene project. On Jan 27, 2011 this sequence version replaced NM_001039348.1. Transcript Variant: This variant (2) represents the longer transcript. Variants 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC098561.1, AK290599.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:12242346) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTTGAAAG CCCTTTTCCT AACTATGCTG ACTCTGGCGC TGGTCAAGTC ACAGGACACC 
GAAGAAACCA TCACGTACAC GCAATGCACT GACGGATATG AGTGGGATCC TGTGAGACAG
CAATGCAAAG ATATTGATGA ATGTGACATT GTCCCAGACG CTTGTAAAGG TGGAATGAAG
TGTGTCAACC ACTATGGAGG ATACCTCTGC CTTCCGAAAA CAGCCCAGAT TATTGTCAAT
AATGAACAGC CTCAGCAGGA AACACAACCA GCAGAAGGAA CCTCAGGGGC AACCACCGGG
GTTGTAGCTG CCAGCAGCAT GGCAACCAGT GGAGTGTTGC CCGGGGGTGG TTTTGTGGCC
AGTGCTGCTG CAGTCGCAGG CCCTGAAATG CAGACTGGCC GAAATAACTT TGTCATCCGG
CGGAACCCAG CTGACCCTCA GCGCATTCCC TCCAACCCTT CCCACCGTAT CCAGTGTGCA
GCAGGCTACG AGCAAAGTGA ACACAACGTG TGCCAAGACA TAGACGAGTG CACTGCAGGG
ACGCACAACT GTAGAGCAGA CCAAGTGTGC ATCAATTTAC GGGGATCCTT TGCATGTCAG
TGCCCTCCTG GATATCAGAA GCGAGGGGAG CAGTGCGTAG ACATAGATGA ATGTACCATC
CCTCCATATT GCCACCAAAG ATGCGTGAAT ACACCAGGCT CATTTTATTG CCAGTGCAGT
CCTGGGTTTC AATTGGCAGC AAACAACTAT ACCTGCGTAG ATATAAATGA ATGTGATGCC
AGCAATCAAT GTGCTCAGCA GTGCTACAAC ATTCTTGGTT CATTCATCTG TCAGTGCAAT
CAAGGATATG AGCTAAGCAG TGACAGGCTC AACTGTGAAG ACATTGATGA ATGCAGAACC
TCAAGCTACC TGTGTCAATA TCAATGTGTC AATGAACCTG GGAAATTCTC ATGTATGTGC
CCCCAGGGAT ACCAAGTGGT GAGAAGTAGA ACATGTCAAG ATATAAATGA GTGTGAGACC
ACAAATGAAT GCCGGGAGGA TGAAATGTGT TGGAATTATC ATGGCGGCTT CCGTTGTTAT
CCACGAAATC CTTGTCAAGA TCCCTACATT CTAACACCAG AGAACCGATG TGTTTGCCCA
GTCTCAAATG CCATGTGCCG AGAACTGCCC CAGTCAATAG TCTACAAATA CATGAGCATC
CGATCTGATA GGTCTGTGCC ATCAGACATC TTCCAGATAC AGGCCACAAC TATTTATGCC
AACACCATCA ATACTTTTCG GATTAAATCT GGAAATGAAA ATGGAGAGTT CTACCTACGA
CAAACAAGTC CTGTAAGTGC AATGCTTGTG CTCGTGAAGT CATTATCAGG ACCAAGAGAA
CATATCGTGG ACCTGGAGAT GCTGACAGTC AGCAGTATAG GGACCTTCCG CACAAGCTCT
GTGTTAAGAT TGACAATAAT AGTGGGGCCA TTTTCATTTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001034437.1
CDS502..1983
Misc Feature(1)19..21
Misc Feature(2)1018..>1119
Misc Feature(3)order(1018..1020,1027..1029,1075..1077)
Misc Feature(4)1141..1233
Misc Feature(5)order(1141..1143,1150..1152,1189..1191)
Misc Feature(6)1201..1272
Misc Feature(7)1276..1980
Misc Feature(8)1321..1392
Misc Feature(9)<1375..1473
Misc Feature(10)1501..>1578
Exon (1)1..453
Exon (2)454..494
Exon (3)495..582
Exon (4)583..631
Exon (5)632..1018
Exon (6)1019..1141
Exon (7)1142..1261
Exon (8)1262..1381
Exon (9)1382..1501
Exon (10)1502..1625
Exon (11)1626..1821
Exon (12)1822..3094
Translation

Target ORF information:

RefSeq Version NM_001039348.2
Organism Homo sapiens(Human)
Definition Homo sapiens EGF containing fibulin like extracellular matrix protein 1 (EFEMP1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001039348.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTTGAAAG CCCTTTTCCT AACTATGCTG ACTCTGGCGC TGGTCAAGTC ACAGGACACC 
GAAGAAACCA TCACGTACAC GCAATGCACT GACGGATATG AGTGGGATCC TGTGAGACAG
CAATGCAAAG ATATTGATGA ATGTGACATT GTCCCAGACG CTTGTAAAGG TGGAATGAAG
TGTGTCAACC ACTATGGAGG ATACCTCTGC CTTCCGAAAA CAGCCCAGAT TATTGTCAAT
AATGAACAGC CTCAGCAGGA AACACAACCA GCAGAAGGAA CCTCAGGGGC AACCACCGGG
GTTGTAGCTG CCAGCAGCAT GGCAACCAGT GGAGTGTTGC CCGGGGGTGG TTTTGTGGCC
AGTGCTGCTG CAGTCGCAGG CCCTGAAATG CAGACTGGCC GAAATAACTT TGTCATCCGG
CGGAACCCAG CTGACCCTCA GCGCATTCCC TCCAACCCTT CCCACCGTAT CCAGTGTGCA
GCAGGCTACG AGCAAAGTGA ACACAACGTG TGCCAAGACA TAGACGAGTG CACTGCAGGG
ACGCACAACT GTAGAGCAGA CCAAGTGTGC ATCAATTTAC GGGGATCCTT TGCATGTCAG
TGCCCTCCTG GATATCAGAA GCGAGGGGAG CAGTGCGTAG ACATAGATGA ATGTACCATC
CCTCCATATT GCCACCAAAG ATGCGTGAAT ACACCAGGCT CATTTTATTG CCAGTGCAGT
CCTGGGTTTC AATTGGCAGC AAACAACTAT ACCTGCGTAG ATATAAATGA ATGTGATGCC
AGCAATCAAT GTGCTCAGCA GTGCTACAAC ATTCTTGGTT CATTCATCTG TCAGTGCAAT
CAAGGATATG AGCTAAGCAG TGACAGGCTC AACTGTGAAG ACATTGATGA ATGCAGAACC
TCAAGCTACC TGTGTCAATA TCAATGTGTC AATGAACCTG GGAAATTCTC ATGTATGTGC
CCCCAGGGAT ACCAAGTGGT GAGAAGTAGA ACATGTCAAG ATATAAATGA GTGTGAGACC
ACAAATGAAT GCCGGGAGGA TGAAATGTGT TGGAATTATC ATGGCGGCTT CCGTTGTTAT
CCACGAAATC CTTGTCAAGA TCCCTACATT CTAACACCAG AGAACCGATG TGTTTGCCCA
GTCTCAAATG CCATGTGCCG AGAACTGCCC CAGTCAATAG TCTACAAATA CATGAGCATC
CGATCTGATA GGTCTGTGCC ATCAGACATC TTCCAGATAC AGGCCACAAC TATTTATGCC
AACACCATCA ATACTTTTCG GATTAAATCT GGAAATGAAA ATGGAGAGTT CTACCTACGA
CAAACAAGTC CTGTAAGTGC AATGCTTGTG CTCGTGAAGT CATTATCAGG ACCAAGAGAA
CATATCGTGG ACCTGGAGAT GCTGACAGTC AGCAGTATAG GGACCTTCCG CACAAGCTCT
GTGTTAAGAT TGACAATAAT AGTGGGGCCA TTTTCATTTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22367
Clone ID Related Accession (Same CDS sequence) NM_001039348.2 , NM_001039349.2
Accession Version NM_001039349.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product EGF-containing fibulin-like extracellular matrix protein 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA264704.1, BC014410.1, AC010895.8 and U03877.1. On Jan 27, 2011 this sequence version replaced NM_001039349.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 2. Variants 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293058.1, BC014410.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTTGAAAG CCCTTTTCCT AACTATGCTG ACTCTGGCGC TGGTCAAGTC ACAGGACACC 
GAAGAAACCA TCACGTACAC GCAATGCACT GACGGATATG AGTGGGATCC TGTGAGACAG
CAATGCAAAG ATATTGATGA ATGTGACATT GTCCCAGACG CTTGTAAAGG TGGAATGAAG
TGTGTCAACC ACTATGGAGG ATACCTCTGC CTTCCGAAAA CAGCCCAGAT TATTGTCAAT
AATGAACAGC CTCAGCAGGA AACACAACCA GCAGAAGGAA CCTCAGGGGC AACCACCGGG
GTTGTAGCTG CCAGCAGCAT GGCAACCAGT GGAGTGTTGC CCGGGGGTGG TTTTGTGGCC
AGTGCTGCTG CAGTCGCAGG CCCTGAAATG CAGACTGGCC GAAATAACTT TGTCATCCGG
CGGAACCCAG CTGACCCTCA GCGCATTCCC TCCAACCCTT CCCACCGTAT CCAGTGTGCA
GCAGGCTACG AGCAAAGTGA ACACAACGTG TGCCAAGACA TAGACGAGTG CACTGCAGGG
ACGCACAACT GTAGAGCAGA CCAAGTGTGC ATCAATTTAC GGGGATCCTT TGCATGTCAG
TGCCCTCCTG GATATCAGAA GCGAGGGGAG CAGTGCGTAG ACATAGATGA ATGTACCATC
CCTCCATATT GCCACCAAAG ATGCGTGAAT ACACCAGGCT CATTTTATTG CCAGTGCAGT
CCTGGGTTTC AATTGGCAGC AAACAACTAT ACCTGCGTAG ATATAAATGA ATGTGATGCC
AGCAATCAAT GTGCTCAGCA GTGCTACAAC ATTCTTGGTT CATTCATCTG TCAGTGCAAT
CAAGGATATG AGCTAAGCAG TGACAGGCTC AACTGTGAAG ACATTGATGA ATGCAGAACC
TCAAGCTACC TGTGTCAATA TCAATGTGTC AATGAACCTG GGAAATTCTC ATGTATGTGC
CCCCAGGGAT ACCAAGTGGT GAGAAGTAGA ACATGTCAAG ATATAAATGA GTGTGAGACC
ACAAATGAAT GCCGGGAGGA TGAAATGTGT TGGAATTATC ATGGCGGCTT CCGTTGTTAT
CCACGAAATC CTTGTCAAGA TCCCTACATT CTAACACCAG AGAACCGATG TGTTTGCCCA
GTCTCAAATG CCATGTGCCG AGAACTGCCC CAGTCAATAG TCTACAAATA CATGAGCATC
CGATCTGATA GGTCTGTGCC ATCAGACATC TTCCAGATAC AGGCCACAAC TATTTATGCC
AACACCATCA ATACTTTTCG GATTAAATCT GGAAATGAAA ATGGAGAGTT CTACCTACGA
CAAACAAGTC CTGTAAGTGC AATGCTTGTG CTCGTGAAGT CATTATCAGG ACCAAGAGAA
CATATCGTGG ACCTGGAGAT GCTGACAGTC AGCAGTATAG GGACCTTCCG CACAAGCTCT
GTGTTAAGAT TGACAATAAT AGTGGGGCCA TTTTCATTTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001034438.1
CDS461..1942
Misc Feature(1)65..67
Misc Feature(2)977..>1078
Misc Feature(3)order(977..979,986..988,1034..1036)
Misc Feature(4)1100..1192
Misc Feature(5)order(1100..1102,1109..1111,1148..1150)
Misc Feature(6)1160..1231
Misc Feature(7)1235..1939
Misc Feature(8)1280..1351
Misc Feature(9)<1334..1432
Misc Feature(10)1460..>1537
Exon (1)1..453
Exon (2)454..541
Exon (3)542..590
Exon (4)591..977
Exon (5)978..1100
Exon (6)1101..1220
Exon (7)1221..1340
Exon (8)1341..1460
Exon (9)1461..1584
Exon (10)1585..1780
Exon (11)1781..3053
Translation

Target ORF information:

RefSeq Version NM_001039349.2
Organism Homo sapiens(Human)
Definition Homo sapiens EGF containing fibulin like extracellular matrix protein 1 (EFEMP1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001039349.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTTGAAAG CCCTTTTCCT AACTATGCTG ACTCTGGCGC TGGTCAAGTC ACAGGACACC 
GAAGAAACCA TCACGTACAC GCAATGCACT GACGGATATG AGTGGGATCC TGTGAGACAG
CAATGCAAAG ATATTGATGA ATGTGACATT GTCCCAGACG CTTGTAAAGG TGGAATGAAG
TGTGTCAACC ACTATGGAGG ATACCTCTGC CTTCCGAAAA CAGCCCAGAT TATTGTCAAT
AATGAACAGC CTCAGCAGGA AACACAACCA GCAGAAGGAA CCTCAGGGGC AACCACCGGG
GTTGTAGCTG CCAGCAGCAT GGCAACCAGT GGAGTGTTGC CCGGGGGTGG TTTTGTGGCC
AGTGCTGCTG CAGTCGCAGG CCCTGAAATG CAGACTGGCC GAAATAACTT TGTCATCCGG
CGGAACCCAG CTGACCCTCA GCGCATTCCC TCCAACCCTT CCCACCGTAT CCAGTGTGCA
GCAGGCTACG AGCAAAGTGA ACACAACGTG TGCCAAGACA TAGACGAGTG CACTGCAGGG
ACGCACAACT GTAGAGCAGA CCAAGTGTGC ATCAATTTAC GGGGATCCTT TGCATGTCAG
TGCCCTCCTG GATATCAGAA GCGAGGGGAG CAGTGCGTAG ACATAGATGA ATGTACCATC
CCTCCATATT GCCACCAAAG ATGCGTGAAT ACACCAGGCT CATTTTATTG CCAGTGCAGT
CCTGGGTTTC AATTGGCAGC AAACAACTAT ACCTGCGTAG ATATAAATGA ATGTGATGCC
AGCAATCAAT GTGCTCAGCA GTGCTACAAC ATTCTTGGTT CATTCATCTG TCAGTGCAAT
CAAGGATATG AGCTAAGCAG TGACAGGCTC AACTGTGAAG ACATTGATGA ATGCAGAACC
TCAAGCTACC TGTGTCAATA TCAATGTGTC AATGAACCTG GGAAATTCTC ATGTATGTGC
CCCCAGGGAT ACCAAGTGGT GAGAAGTAGA ACATGTCAAG ATATAAATGA GTGTGAGACC
ACAAATGAAT GCCGGGAGGA TGAAATGTGT TGGAATTATC ATGGCGGCTT CCGTTGTTAT
CCACGAAATC CTTGTCAAGA TCCCTACATT CTAACACCAG AGAACCGATG TGTTTGCCCA
GTCTCAAATG CCATGTGCCG AGAACTGCCC CAGTCAATAG TCTACAAATA CATGAGCATC
CGATCTGATA GGTCTGTGCC ATCAGACATC TTCCAGATAC AGGCCACAAC TATTTATGCC
AACACCATCA ATACTTTTCG GATTAAATCT GGAAATGAAA ATGGAGAGTT CTACCTACGA
CAAACAAGTC CTGTAAGTGC AATGCTTGTG CTCGTGAAGT CATTATCAGG ACCAAGAGAA
CATATCGTGG ACCTGGAGAT GCTGACAGTC AGCAGTATAG GGACCTTCCG CACAAGCTCT
GTGTTAAGAT TGACAATAAT AGTGGGGCCA TTTTCATTTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu89157
Clone ID Related Accession (Same CDS sequence) XM_017003586.1
Accession Version XM_017003586.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1242bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product EGF-containing fibulin-like extracellular matrix protein 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTTGAAAG CCCTTTTCCT AACTATGCTG ACTCTGGCGC TGGTCAAGTC ACAGGACACC 
GAAGAAACCA TCACGTACAC GCAATGCACT GACGGATATG AGTGGGATCC TGTGAGACAG
CAATGCAAAG ATATTGATGA ATGTGACATT GTCCCAGACG CTTGTAAAGG TGGAATGAAG
TGTGTCAACC ACTATGGAGG ATACCTCTGC CTTCCGAAAA CAGCCCAGAT TATTGTCAAT
AATGAACAGC CTCAGCAGGA AACACAACCA GCAGAAGGAA CCTCAGGGGC AACCACCGGG
GTTGTAGCTG CCAGCAGCAT GGCAACCAGT GGAGTGTTGC CCGGGGGTGG TTTTGTGGCC
AGTGCTGCTG CAGTCGCAGG CCCTGAAATG CAGACTGGCC GAAATAACTT TGTCATCCGG
CGGAACCCAG CTGACCCTCA GCGCATTCCC TCCAACCCTT CCCACCGTAT CCAGTGTGCA
GCAGGCTACG AGCAAAGTGA ACACAACGTG TGCCAAGACA TAGACGAGTG CACTGCAGGG
ACGCACAACT GTAGAGCAGA CCAAGTGTGC ATCAATTTAC GGGGATCCTT TGCATGTCAG
TGCCCTCCTG GATATCAGAA GCGAGGGGAG CAGTGCGTAG ACATTGATGA ATGCAGAACC
TCAAGCTACC TGTGTCAATA TCAATGTGTC AATGAACCTG GGAAATTCTC ATGTATGTGC
CCCCAGGGAT ACCAAGTGGT GAGAAGTAGA ACATGTCAAG ATATAAATGA GTGTGAGACC
ACAAATGAAT GCCGGGAGGA TGAAATGTGT TGGAATTATC ATGGCGGCTT CCGTTGTTAT
CCACGAAATC CTTGTCAAGA TCCCTACATT CTAACACCAG AGAACCGATG TGTTTGCCCA
GTCTCAAATG CCATGTGCCG AGAACTGCCC CAGTCAATAG TCTACAAATA CATGAGCATC
CGATCTGATA GGTCTGTGCC ATCAGACATC TTCCAGATAC AGGCCACAAC TATTTATGCC
AACACCATCA ATACTTTTCG GATTAAATCT GGAAATGAAA ATGGAGAGTT CTACCTACGA
CAAACAAGTC CTGTAAGTGC AATGCTTGTG CTCGTGAAGT CATTATCAGG ACCAAGAGAA
CATATCGTGG ACCTGGAGAT GCTGACAGTC AGCAGTATAG GGACCTTCCG CACAAGCTCT
GTGTTAAGAT TGACAATAAT AGTGGGGCCA TTTTCATTTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016859075.1
CDS437..1678
Misc Feature(1)953..>1054
Misc Feature(2)order(953..955,962..964,1010..1012)
Misc Feature(3)1076..1195
Misc Feature(4)1196..1330
Misc Feature(5)order(1196..1198,1205..1207,1250..1252)
Translation

Target ORF information:

RefSeq Version XM_017003586.1
Organism Homo sapiens(Human)
Definition Homo sapiens EGF containing fibulin like extracellular matrix protein 1 (EFEMP1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017003586.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTTGAAAG CCCTTTTCCT AACTATGCTG ACTCTGGCGC TGGTCAAGTC ACAGGACACC 
GAAGAAACCA TCACGTACAC GCAATGCACT GACGGATATG AGTGGGATCC TGTGAGACAG
CAATGCAAAG ATATTGATGA ATGTGACATT GTCCCAGACG CTTGTAAAGG TGGAATGAAG
TGTGTCAACC ACTATGGAGG ATACCTCTGC CTTCCGAAAA CAGCCCAGAT TATTGTCAAT
AATGAACAGC CTCAGCAGGA AACACAACCA GCAGAAGGAA CCTCAGGGGC AACCACCGGG
GTTGTAGCTG CCAGCAGCAT GGCAACCAGT GGAGTGTTGC CCGGGGGTGG TTTTGTGGCC
AGTGCTGCTG CAGTCGCAGG CCCTGAAATG CAGACTGGCC GAAATAACTT TGTCATCCGG
CGGAACCCAG CTGACCCTCA GCGCATTCCC TCCAACCCTT CCCACCGTAT CCAGTGTGCA
GCAGGCTACG AGCAAAGTGA ACACAACGTG TGCCAAGACA TAGACGAGTG CACTGCAGGG
ACGCACAACT GTAGAGCAGA CCAAGTGTGC ATCAATTTAC GGGGATCCTT TGCATGTCAG
TGCCCTCCTG GATATCAGAA GCGAGGGGAG CAGTGCGTAG ACATTGATGA ATGCAGAACC
TCAAGCTACC TGTGTCAATA TCAATGTGTC AATGAACCTG GGAAATTCTC ATGTATGTGC
CCCCAGGGAT ACCAAGTGGT GAGAAGTAGA ACATGTCAAG ATATAAATGA GTGTGAGACC
ACAAATGAAT GCCGGGAGGA TGAAATGTGT TGGAATTATC ATGGCGGCTT CCGTTGTTAT
CCACGAAATC CTTGTCAAGA TCCCTACATT CTAACACCAG AGAACCGATG TGTTTGCCCA
GTCTCAAATG CCATGTGCCG AGAACTGCCC CAGTCAATAG TCTACAAATA CATGAGCATC
CGATCTGATA GGTCTGTGCC ATCAGACATC TTCCAGATAC AGGCCACAAC TATTTATGCC
AACACCATCA ATACTTTTCG GATTAAATCT GGAAATGAAA ATGGAGAGTT CTACCTACGA
CAAACAAGTC CTGTAAGTGC AATGCTTGTG CTCGTGAAGT CATTATCAGG ACCAAGAGAA
CATATCGTGG ACCTGGAGAT GCTGACAGTC AGCAGTATAG GGACCTTCCG CACAAGCTCT
GTGTTAAGAT TGACAATAAT AGTGGGGCCA TTTTCATTTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu35325
Clone ID Related Accession (Same CDS sequence) XM_005264205.4
Accession Version XM_005264205.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1632bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product EGF-containing fibulin-like extracellular matrix protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005264205.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTCAACGT GTCCTCTCTG GCGTGGGGTC CCCGCGAGTC TGGGAAACGA GGAGCTGGCA 
AGGGAGGGCA AGCGGGGGCG GCAAAGAGGG CCCCTGGAGC AGGGGGCGCG GACCTCGCTG
CGCTGCGCGC TCTACCGCCG GGCTCGCAAC GCTGGGCTCA GCGCTCGCGC CTCCCTCAGC
TCTCTCCTCC GCCCCCCTTC GCCCTCCCCC TTTCCCTCCC TTTCTCCTCC TCCTCCTGCC
GCCGCGGCCG CTGCCGGACT TCGCCAGATC AGACCCACGG GGCTGCCCTC CCCTGCGCAC
TCCCCTCGCT GCCCGGGCCC GGAGCGCAGC GCGGCCGCAC AGGTATTTTT GCTGTGCTGT
GCAAGGAACT CTGCTAGCTC AAGATTCACA ATGTTGAAAG CCCTTTTCCT AACTATGCTG
ACTCTGGCGC TGGTCAAGTC ACAGGACACC GAAGAAACCA TCACGTACAC GCAATGCACT
GACGGATATG AGTGGGATCC TGTGAGACAG CAATGCAAAG ATATTGATGA ATGTGACATT
GTCCCAGACG CTTGTAAAGG TGGAATGAAG TGTGTCAACC ACTATGGAGG ATACCTCTGC
CTTCCGAAAA CAGCCCAGAT TATTGTCAAT AATGAACAGC CTCAGCAGGA AACACAACCA
GCAGAAGGAA CCTCAGGGGC AACCACCGGG GTTGTAGCTG CCAGCAGCAT GGCAACCAGT
GGAGTGTTGC CCGGGGGTGG TTTTGTGGCC AGTGCTGCTG CAGTCGCAGG CCCTGAAATG
CAGACTGGCC GAAATAACTT TGTCATCCGG CGGAACCCAG CTGACCCTCA GCGCATTCCC
TCCAACCCTT CCCACCGTAT CCAGTGTGCA GCAGGCTACG AGCAAAGTGA ACACAACGTG
TGCCAAGACA TAGACGAGTG CACTGCAGGG ACGCACAACT GTAGAGCAGA CCAAGTGTGC
ATCAATTTAC GGGGATCCTT TGCATGTCAG TGCCCTCCTG GATATCAGAA GCGAGGGGAG
CAGTGCGTAG ACATTGATGA ATGCAGAACC TCAAGCTACC TGTGTCAATA TCAATGTGTC
AATGAACCTG GGAAATTCTC ATGTATGTGC CCCCAGGGAT ACCAAGTGGT GAGAAGTAGA
ACATGTCAAG ATATAAATGA GTGTGAGACC ACAAATGAAT GCCGGGAGGA TGAAATGTGT
TGGAATTATC ATGGCGGCTT CCGTTGTTAT CCACGAAATC CTTGTCAAGA TCCCTACATT
CTAACACCAG AGAACCGATG TGTTTGCCCA GTCTCAAATG CCATGTGCCG AGAACTGCCC
CAGTCAATAG TCTACAAATA CATGAGCATC CGATCTGATA GGTCTGTGCC ATCAGACATC
TTCCAGATAC AGGCCACAAC TATTTATGCC AACACCATCA ATACTTTTCG GATTAAATCT
GGAAATGAAA ATGGAGAGTT CTACCTACGA CAAACAAGTC CTGTAAGTGC AATGCTTGTG
CTCGTGAAGT CATTATCAGG ACCAAGAGAA CATATCGTGG ACCTGGAGAT GCTGACAGTC
AGCAGTATAG GGACCTTCCG CACAAGCTCT GTGTTAAGAT TGACAATAAT AGTGGGGCCA
TTTTCATTTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005264262.1
CDS112..1743
Misc Feature(1)631..>714
Misc Feature(2)1018..>1119
Misc Feature(3)order(1018..1020,1027..1029,1075..1077)
Misc Feature(4)1141..1260
Misc Feature(5)order(1141..1143,1150..1152,1192..1194)
Misc Feature(6)1261..1395
Misc Feature(7)order(1261..1263,1270..1272,1315..1317)
Translation

Target ORF information:

RefSeq Version XM_005264205.4
Organism Homo sapiens(Human)
Definition Homo sapiens EGF containing fibulin like extracellular matrix protein 1 (EFEMP1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005264205.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTCAACGT GTCCTCTCTG GCGTGGGGTC CCCGCGAGTC TGGGAAACGA GGAGCTGGCA 
AGGGAGGGCA AGCGGGGGCG GCAAAGAGGG CCCCTGGAGC AGGGGGCGCG GACCTCGCTG
CGCTGCGCGC TCTACCGCCG GGCTCGCAAC GCTGGGCTCA GCGCTCGCGC CTCCCTCAGC
TCTCTCCTCC GCCCCCCTTC GCCCTCCCCC TTTCCCTCCC TTTCTCCTCC TCCTCCTGCC
GCCGCGGCCG CTGCCGGACT TCGCCAGATC AGACCCACGG GGCTGCCCTC CCCTGCGCAC
TCCCCTCGCT GCCCGGGCCC GGAGCGCAGC GCGGCCGCAC AGGTATTTTT GCTGTGCTGT
GCAAGGAACT CTGCTAGCTC AAGATTCACA ATGTTGAAAG CCCTTTTCCT AACTATGCTG
ACTCTGGCGC TGGTCAAGTC ACAGGACACC GAAGAAACCA TCACGTACAC GCAATGCACT
GACGGATATG AGTGGGATCC TGTGAGACAG CAATGCAAAG ATATTGATGA ATGTGACATT
GTCCCAGACG CTTGTAAAGG TGGAATGAAG TGTGTCAACC ACTATGGAGG ATACCTCTGC
CTTCCGAAAA CAGCCCAGAT TATTGTCAAT AATGAACAGC CTCAGCAGGA AACACAACCA
GCAGAAGGAA CCTCAGGGGC AACCACCGGG GTTGTAGCTG CCAGCAGCAT GGCAACCAGT
GGAGTGTTGC CCGGGGGTGG TTTTGTGGCC AGTGCTGCTG CAGTCGCAGG CCCTGAAATG
CAGACTGGCC GAAATAACTT TGTCATCCGG CGGAACCCAG CTGACCCTCA GCGCATTCCC
TCCAACCCTT CCCACCGTAT CCAGTGTGCA GCAGGCTACG AGCAAAGTGA ACACAACGTG
TGCCAAGACA TAGACGAGTG CACTGCAGGG ACGCACAACT GTAGAGCAGA CCAAGTGTGC
ATCAATTTAC GGGGATCCTT TGCATGTCAG TGCCCTCCTG GATATCAGAA GCGAGGGGAG
CAGTGCGTAG ACATTGATGA ATGCAGAACC TCAAGCTACC TGTGTCAATA TCAATGTGTC
AATGAACCTG GGAAATTCTC ATGTATGTGC CCCCAGGGAT ACCAAGTGGT GAGAAGTAGA
ACATGTCAAG ATATAAATGA GTGTGAGACC ACAAATGAAT GCCGGGAGGA TGAAATGTGT
TGGAATTATC ATGGCGGCTT CCGTTGTTAT CCACGAAATC CTTGTCAAGA TCCCTACATT
CTAACACCAG AGAACCGATG TGTTTGCCCA GTCTCAAATG CCATGTGCCG AGAACTGCCC
CAGTCAATAG TCTACAAATA CATGAGCATC CGATCTGATA GGTCTGTGCC ATCAGACATC
TTCCAGATAC AGGCCACAAC TATTTATGCC AACACCATCA ATACTTTTCG GATTAAATCT
GGAAATGAAA ATGGAGAGTT CTACCTACGA CAAACAAGTC CTGTAAGTGC AATGCTTGTG
CTCGTGAAGT CATTATCAGG ACCAAGAGAA CATATCGTGG ACCTGGAGAT GCTGACAGTC
AGCAGTATAG GGACCTTCCG CACAAGCTCT GTGTTAAGAT TGACAATAAT AGTGGGGCCA
TTTTCATTTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Comparison of the Diagnostic Performance of Fibulin-3 and Mesothelin in Patients with Pleural Effusions from Malignant Mesothelioma.
Anticancer research37(3)1387-1391(2017 03)
Battolla E,Canessa PA,Ferro P,Franceschini MC,Fontana V,Dessanti P,Pinelli V,Morabito A,Fedeli F,Pistillo MP,Roncella S


book

Assessing the association between EFEMP1 rs3791679 polymorphism and risk of glioma in a Chinese Han population.
Genetics and molecular research : GMR15(3)(2016 Aug)
Jiang N,Peng YP,Wang XY,Dou CW,He WM


book

EFEMP1 inhibits migration of hepatocellular carcinoma by regulating MMP2 and MMP9 via ERK1/2 activity.
Oncology reports35(6)3489-95(2016 Jun)
Dou CY,Cao CJ,Wang Z,Zhang RH,Huang LL,Lian JY,Xie WL,Wang LT


book

Transcriptional regulation and expression of the dominant drusen gene FBLN3 (EFEMP1) in mammalian retina.
Investigative ophthalmology & visual science44(11)4613-21(2003 Nov)
Blackburn J,Tarttelin EE,Gregory-Evans CY,Moosajee M,Gregory-Evans K


book

Aberrant accumulation of EFEMP1 underlies drusen formation in Malattia Leventinese and age-related macular degeneration.
Proceedings of the National Academy of Sciences of the United States of America99(20)13067-72(2002 Oct)
Marmorstein LY,Munier FL,Arsenijevic Y,Schorderet DF,McLaughlin PJ,Chung D,Traboulsi E,Marmorstein AD


 
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