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BRAT1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol BRAT1
Entrez Gene ID 221927
Full Name BRCA1 associated ATM activator 1
Synonyms BAAT1,C7orf27,RMFSL
General protein information
Preferred Names

BRCA1 associated ATM activator 1

Names

BRCA1-associated ATM activator 1
BRCA1-associated protein required for ATM activation protein 1
HEAT repeat-containing protein C7orf27

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

7

7p22.3

Summary The protein encoded by this ubiquitously expressed gene interacts with the tumor suppressing BRCA1 (breast cancer 1) protein and and the ATM (ataxia telangiectasia mutated) protein. ATM is thought to be a master controller of cell cycle checkpoint signalling pathways that are required for cellular responses to DNA damage such as double-strand breaks that are induced by ionizing radiation and complexes with BRCA1 in the multi-protein complex BASC (BRAC1-associated genome surveillance complex). The protein encoded by this gene is thought to play a role in the DNA damage pathway regulated by BRCA1 and ATM. [provided by RefSeq, Mar 2012].
Disorder MIM:

614506


mRNA and Protein(s)

mRNA Protein Name
NM_001350627.1 NP_001337556.1 BRCA1-associated ATM activator 1 isoform 3
NM_152743.3 NP_689956.2 BRCA1-associated ATM activator 1 isoform 2
XM_017011834.1 XP_016867323.1 BRCA1-associated ATM activator 1 isoform X6
XM_011515184.2 XP_011513486.1 BRCA1-associated ATM activator 1 isoform X7
XM_017011836.1 XP_016867325.1 BRCA1-associated ATM activator 1 isoform X10
XM_011515178.1 XP_011513480.1 BRCA1-associated ATM activator 1 isoform X1
XM_011515179.2 XP_011513481.1 BRCA1-associated ATM activator 1 isoform X2
NM_001350626.1 NP_001337555.1 BRCA1-associated ATM activator 1 isoform 1
XM_011515182.2 XP_011513484.1 BRCA1-associated ATM activator 1 isoform X1
XM_017011833.1 XP_016867322.1 BRCA1-associated ATM activator 1 isoform X4
XM_011515177.2 XP_011513479.1 BRCA1-associated ATM activator 1 isoform X1
XM_011515186.2 XP_011513488.1 BRCA1-associated ATM activator 1 isoform X9
XM_011515181.2 XP_011513483.1 BRCA1-associated ATM activator 1 isoform X5
XM_011515183.1 XP_011513485.1 BRCA1-associated ATM activator 1 isoform X7



Homo sapiens (human) BRAT1 NP_689956.2
Mus musculus (house mouse) Brat1 NP_001263216.1
Danio rerio (zebrafish) brat1 NP_001035395.1
Xenopus tropicalis (tropical clawed frog) brat1 XP_002932270.2
Bos taurus (cattle) BRAT1 NP_001193384.1
Macaca mulatta (rhesus monkey) LOC699605 XP_001087105.1
Pan troglodytes (chimpanzee) BRAT1 XP_518937.3
Rattus norvegicus (Norway rat) Brat1 XP_006249032.1
Gallus gallus (chicken) BRAT1 XP_001233206.3
Canis lupus familiaris (dog) BRAT1 XP_005621213.1


Related articles in PubMed

Early-Onset Severe Encephalopathy with Epilepsy: The BRAT1 Gene Should Be Added to the List of Causes.
van de Pol LA, Wolf NI, van Weissenbruch MM, Stam CJ, Weiss JM, Waisfisz Q, Kevelam SH, Bugiani M, van de Kamp JM, van der Knaap MS
Neuropediatrics46(6)392-400(2015 Dec)

Nedd4 family interacting protein 1 (Ndfip1) is required for ubiquitination and nuclear trafficking of BRCA1-associated ATM activator 1 (BRAT1) during the DNA damage response.
Low LH, Chow YL, Li Y, Goh CP, Putz U, Silke J, Ouchi T, Howitt J, Tan SS
The Journal of biological chemistry290(11)7141-50(2015 Mar)

Compound heterozygous BRAT1 mutations cause familial Ohtahara syndrome with hypertonia and microcephaly.
Saitsu H, Yamashita S, Tanaka Y, Tsurusaki Y, Nakashima M, Miyake N, Matsumoto N
Journal of human genetics59(12)687-90(2014 Dec)

BRAT1 deficiency causes increased glucose metabolism and mitochondrial malfunction.
So EY, Ouchi T
BMC cancer14548(2014 Jul)

The Potential Role of BRCA1-Associated ATM Activator-1 (BRAT1) in Regulation of mTOR.
So EY, Ouchi T
Journal of cancer biology & research1(1)(2013 Jul-Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We conclude that BRAT1 should be added to the growing list of genes related to early-onset severe encephalopathy with epilepsy.
Title: Early-Onset Severe Encephalopathy with Epilepsy: The BRAT1 Gene Should Be Added to the List of Causes.

Ndfip1 is required during stress for ubiquitinating and trafficking BRAT1 into the nucleus.
Title: Nedd4 family interacting protein 1 (Ndfip1) is required for ubiquitination and nuclear trafficking of BRCA1-associated ATM activator 1 (BRAT1) during the DNA damage response.

Our results further support that mutations of BRAT1 could lead to epileptic encephalopathy.
Title: Compound heterozygous BRAT1 mutations cause familial Ohtahara syndrome with hypertonia and microcephaly.

Findings suggest novel roles of BRAT1 in cell proliferation and mitochondrial functions.
Title: BRAT1 deficiency causes increased glucose metabolism and mitochondrial malfunction.

Data on crystal structure of BRCA1 binding with phosphopeptides suggest that C-terminal domain of BRCA1 interacts with BAAT1 and ATRIP with preferences for specific side chains; in BAAT1, phospho-Ser269 and Phe272 are the main interacting residues.
Title: Structural basis for the BRCA1 BRCT interaction with the proteins ATRIP and BAAT1.

The following BRAT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu78068 XM_011515186.2
Latest version!
Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant X12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu101583 NM_001350627.1
Latest version!
Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 10-12 $307.30
$439.00
OHu22650 NM_152743.3
Latest version!
Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $265.30
$379.00
OHu41095 NM_001350626.1
Latest version!
Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu78063 XM_011515182.2
Latest version!
Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu78063 XM_011515178.1
Latest version!
Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu78063 XM_011515177.2
Latest version!
Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu78064 XM_011515179.2
Latest version!
Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu78066 XM_011515181.2
Latest version!
Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu78067 XM_011515183.1
Latest version!
Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu78067 XM_011515184.2
Latest version!
Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu97084 XM_017011833.1
Latest version!
Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $475.30
$679.00
OHu97085 XM_017011834.1
Latest version!
Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OHu97086 XM_017011836.1
Latest version!
Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant X13, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu78068
Clone ID Related Accession (Same CDS sequence) XM_011515186.2
Accession Version XM_011515186.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1764bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product BRCA1-associated ATM activator 1 isoform X9
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515186.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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ATGGACCCAG AATGCGCCCA GCTGCTCCCG GCTCTCTGTG CTGTTCTGGT AGATCCCAGG 
CAGCCGGTGG CAGATGACAC CTGTTTGGAG AAGCTCCTGG ACTGGTTTAA AACGGTCACT
GAAGGAGAGT CCAGTGTCGT GCTGCTGCAG GAGCACCCCT GCCTGGTGGA GCTGCTGTCC
CATGTGCTGA AAGTCCAGGA CCTGAGTTCT GGGGTCCTCT CCTTCTCACT GCGCCTGGCA
GGAACCTTCG CAGCCCAGGA AAACTGCTTC CAGTATCTTC AGCAGGGGGA GTTACTACCA
GGGCTCTTTG GGGAGCCAGG ACCCCTCGGC CGAGCAACCT GGGCCGTCCC CACCGTGCGC
AGCGGCTGGA TCCAGGGCCT GCGCTCCCTG GCACAGCACC CCAGCGCCCT GCGCTTCCTG
GCCGACCATG GTGCGGTCGA CACCATCTTC TCCCTGCAGG GAGACTCCAG CCTGTTTGTG
GCCTCGGCGG CCAGTCAGCT CCTGGTGCAC GTCCTGGCTT TGTCCATGCG AGGTGGAGCC
GAGGGGCAGC CCTGCCTGCC GGGGGGTGAC TGGCCCGCGT GTGCCCAGAA GATCATGGAT
CACGTTGAAG AGTCCTTGTG CTCCGCGGCC ACCCCCAAGG TCACTCAGGC CCTGAACGTC
CTGACCACGA CCTTCGGGCG CTGCCAGAGC CCCTGGACGG AAGCCCTGTG GGTGCGGCTG
AGTCCCCGCG TGGCCTGTCT GCTGGAGAGA GACCCCATCC CCGCCGCACA CTCGTTCGTG
GACCTGCTTC TCTGTGTGGC TCGTTCTCCC GTGTTCAGTT CTTCCGACGG CAGCCTGTGG
GAGACAGTGG CGCGGGCTCT GAGCTGCCTG GGTCCCACCC ACATGGGACC CCTGGCTTTG
GGGATCCTGA AGCTCGAGCA CTGTCCACAG GCACTGAGGA CCCAGGCCTT CCAGGTCCTT
CTCCAGCCCC TGGCCTGTGT CCTGAAGGCC ACGGTTCAGG CCCCCGGACC CCCAGGCTTG
CTGGACGGGA CGGCAGACGA TGCCACGACG GTGGACACAC TCCTGGCCTC CAAGTCGTCC
TGCGCCGGCC TCCTGTGCCG CACCCTGGCT CACCTGGAGG AGCTGCAGCC GCTGCCCCAG
CGCCCTTCAC CGTGGCCCCA GGCGTCTCTA CTGGGGGCTA CAGTGACTGT CCTGCGGCTC
TGTGACGGCT CGGCTGCCCC TGCCTCCAGT GTGGGGGGCC ACCTCTGTGG GACCCTGGCG
GGCTGCGTCC GGGTCCAGCG AGCAGCCCTC GACTTCCTGG GGACGCTGTC ACAGGGGACA
GGCCCCCAGG AGCTGGTGAC GCAGGCGCTT GCTGTCCTCC TGGAGTGCCT CGAGAGCCCC
GGCTCCAGCC CCACGGCCTC TGCGGATGGG GCCTTCACAC AGGGCCACAG AGCTGCTGCT
CAGAGAGCTT CACGATGCAG CCCACCCTAT CTGCTCCAGG TTCTGAAGAA GGCCTTCCAG
GCCACGCTCA GGTGGCTCCT GAGCTCACCC AAGACCCCCG GCTGCTCTGA TCTCGGCCCC
CTCATCCCGC AGTTCCTCAG AGAGCTGTTC CCTGTGCTGC AGAAACGCCT GTGCCACCCC
TGCTGGGAGG TGAGGGACTC CGCCCTCGAG TTCCTGACCC AGCTGAGCAG GCACTGGGGA
GAGCCTGTTC CTGGAGCTCC TGCACATCCT CTCCGTAGAC TCGGAGGGCT TCCCACGGCG
GGCGGTCATG CAAGTCTTCA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011513488.1
CDS248..2011
Misc Feature(1)order(1526..1531,1538..1540,1547..1552,1559..1561,1649..1654,1661..1663,1670..1672,1676..1678,1685..1687,1766..1771,1778..1780,1787..1792,1883..1888,1895..1897,1904..1909)
Misc Feature(2)1589..1690
Misc Feature(3)1715..1792
Misc Feature(4)1823..1912
Translation

Target ORF information:

RefSeq Version XM_011515186.2
Organism Homo sapiens(Human)
Definition Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant X12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011515186.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGACCCAG AATGCGCCCA GCTGCTCCCG GCTCTCTGTG CTGTTCTGGT AGATCCCAGG 
CAGCCGGTGG CAGATGACAC CTGTTTGGAG AAGCTCCTGG ACTGGTTTAA AACGGTCACT
GAAGGAGAGT CCAGTGTCGT GCTGCTGCAG GAGCACCCCT GCCTGGTGGA GCTGCTGTCC
CATGTGCTGA AAGTCCAGGA CCTGAGTTCT GGGGTCCTCT CCTTCTCACT GCGCCTGGCA
GGAACCTTCG CAGCCCAGGA AAACTGCTTC CAGTATCTTC AGCAGGGGGA GTTACTACCA
GGGCTCTTTG GGGAGCCAGG ACCCCTCGGC CGAGCAACCT GGGCCGTCCC CACCGTGCGC
AGCGGCTGGA TCCAGGGCCT GCGCTCCCTG GCACAGCACC CCAGCGCCCT GCGCTTCCTG
GCCGACCATG GTGCGGTCGA CACCATCTTC TCCCTGCAGG GAGACTCCAG CCTGTTTGTG
GCCTCGGCGG CCAGTCAGCT CCTGGTGCAC GTCCTGGCTT TGTCCATGCG AGGTGGAGCC
GAGGGGCAGC CCTGCCTGCC GGGGGGTGAC TGGCCCGCGT GTGCCCAGAA GATCATGGAT
CACGTTGAAG AGTCCTTGTG CTCCGCGGCC ACCCCCAAGG TCACTCAGGC CCTGAACGTC
CTGACCACGA CCTTCGGGCG CTGCCAGAGC CCCTGGACGG AAGCCCTGTG GGTGCGGCTG
AGTCCCCGCG TGGCCTGTCT GCTGGAGAGA GACCCCATCC CCGCCGCACA CTCGTTCGTG
GACCTGCTTC TCTGTGTGGC TCGTTCTCCC GTGTTCAGTT CTTCCGACGG CAGCCTGTGG
GAGACAGTGG CGCGGGCTCT GAGCTGCCTG GGTCCCACCC ACATGGGACC CCTGGCTTTG
GGGATCCTGA AGCTCGAGCA CTGTCCACAG GCACTGAGGA CCCAGGCCTT CCAGGTCCTT
CTCCAGCCCC TGGCCTGTGT CCTGAAGGCC ACGGTTCAGG CCCCCGGACC CCCAGGCTTG
CTGGACGGGA CGGCAGACGA TGCCACGACG GTGGACACAC TCCTGGCCTC CAAGTCGTCC
TGCGCCGGCC TCCTGTGCCG CACCCTGGCT CACCTGGAGG AGCTGCAGCC GCTGCCCCAG
CGCCCTTCAC CGTGGCCCCA GGCGTCTCTA CTGGGGGCTA CAGTGACTGT CCTGCGGCTC
TGTGACGGCT CGGCTGCCCC TGCCTCCAGT GTGGGGGGCC ACCTCTGTGG GACCCTGGCG
GGCTGCGTCC GGGTCCAGCG AGCAGCCCTC GACTTCCTGG GGACGCTGTC ACAGGGGACA
GGCCCCCAGG AGCTGGTGAC GCAGGCGCTT GCTGTCCTCC TGGAGTGCCT CGAGAGCCCC
GGCTCCAGCC CCACGGCCTC TGCGGATGGG GCCTTCACAC AGGGCCACAG AGCTGCTGCT
CAGAGAGCTT CACGATGCAG CCCACCCTAT CTGCTCCAGG TTCTGAAGAA GGCCTTCCAG
GCCACGCTCA GGTGGCTCCT GAGCTCACCC AAGACCCCCG GCTGCTCTGA TCTCGGCCCC
CTCATCCCGC AGTTCCTCAG AGAGCTGTTC CCTGTGCTGC AGAAACGCCT GTGCCACCCC
TGCTGGGAGG TGAGGGACTC CGCCCTCGAG TTCCTGACCC AGCTGAGCAG GCACTGGGGA
GAGCCTGTTC CTGGAGCTCC TGCACATCCT CTCCGTAGAC TCGGAGGGCT TCCCACGGCG
GGCGGTCATG CAAGTCTTCA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu101583
Clone ID Related Accession (Same CDS sequence) NM_001350627.1
Accession Version NM_001350627.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1941bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-20
Organism Homo sapiens(Human)
Product BRCA1-associated ATM activator 1 isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092488.3. On Apr 20, 2017 this sequence version replaced XM_017011835.1. Transcript Variant: This variant (3) lacks an alternate exon and uses an alternate in-frame splice junction in the 3' coding sequence compared to variant 1. The resulting isoform (3) is shorter at the N-terminus and lacks an internal segment compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.31012.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
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961
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1501
1561
1621
1681
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1801
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1921
ATGCGAGGTG GAGCCGAGGG GCAGCCCTGC CTGCCGGGGG GTGACTGGCC CGCGTGTGCC 
CAGAAGATCA TGGATCACGT TGAAGAGTCC TTGTGCTCCG CGGCCACCCC CAAGGTCACT
CAGGCCCTGA ACGTCCTGAC CACGACCTTC GGGCGCTGCC AGAGCCCCTG GACGGAAGCC
CTGTGGGTGC GGCTGAGTCC CCGCGTGGCC TGTCTGCTGG AGAGAGACCC CATCCCCGCC
GCACACTCGT TCGTGGACCT GCTTCTCTGT GTGGCTCGTT CTCCCGTGTT CAGTTCTTCC
GACGGCAGCC TGTGGGAGAC AGTGGCGCGG GCTCTGAGCT GCCTGGGTCC CACCCACATG
GGACCCCTGG CTTTGGGGAT CCTGAAGCTC GAGCACTGTC CACAGGCACT GAGGACCCAG
GCCTTCCAGG TCCTTCTCCA GCCCCTGGCC TGTGTCCTGA AGGCCACGGT TCAGGCCCCC
GGACCCCCAG GCTTGCTGGA CGGGACGGCA GACGATGCCA CGACGGTGGA CACACTCCTG
GCCTCCAAGT CGTCCTGCGC CGGCCTCCTG TGCCGCACCC TGGCTCACCT GGAGGAGCTG
CAGCCGCTGC CCCAGCGCCC TTCACCGTGG CCCCAGGCGT CTCTACTGGG GGCTACAGTG
ACTGTCCTGC GGCTCTGTGA CGGCTCGGCT GCCCCTGCCT CCAGTGTGGG GGGCCACCTC
TGTGGGACCC TGGCGGGCTG CGTCCGGGTC CAGCGAGCAG CCCTCGACTT CCTGGGGACG
CTGTCACAGG GGACAGGCCC CCAGGAGCTG GTGACGCAGG CGCTTGCTGT CCTCCTGGAG
TGCCTCGAGA GCCCCGGCTC CAGCCCCACG GTTCTGAAGA AGGCCTTCCA GGCCACGCTC
AGGTGGCTCC TGAGCTCACC CAAGACCCCC GGCTGCTCTG ATCTCGGCCC CCTCATCCCG
CAGTTCCTCA GAGAGCTGTT CCCTGTGCTG CAGAAACGCC TGTGCCACCC CTGCTGGGAG
GTGAGGGACT CCGCCCTCGA GTTCCTGACC CAGCTGAGCA GGCACTGGGG AGGACAGGCT
GACTTCAGAT GCGCACTCTT GGCTTCAGAG GTGCCTCAGC TGGCCCTGCA GCTCCTCCAG
GACCCTGAGA GTTATGTCCG AGCGAGTGCA GTGACCGCCA TGGGGCAGCT GTCCAGCCAG
GGCCTGCACG CCCCCACCAG CCCTGAGCAT GCAGAGGCCC GGCAGAGCCT GTTCCTGGAG
CTCCTGCACA TCCTCTCCGT AGACTCGGAG GGCTTCCCAC GGCGGGCGGT CATGCAAGTC
TTCACTGAGT GGCTGCGGGA CGGCCACGCC GACGCGGCCC AGGACACGGA GCAGTTCGTG
GCCACTGTGC TGCAGGCGGC GAGCCGAGAC CTGGACTGGG AGGTCCGCGC CCAGGGCCTG
GAGCTGGCCC TCGTGTTCCT GGGCCAGACT TTGGGGCCGC CGCGTACCCA CTGCCCCTAT
GCCGTGGCCC TACCCGAGGT GGCCCCAGCC CAGCCACTCA CCGAGGCACT GAGGGCTCTC
TGCCACGTGG GGCTCTTTGA CTTCGCCTTT TGTGCCTTGT TTGACTGCGA CCGCCCTGTG
GCGCAGAAGT CTTGTGACCT CCTTCTCTTC CTGAGGGACA AGATTGCTTC CTACAGCAGC
CTGCGGGAGG CCAGGGGCAG CCCCAACACT GCCTCCGCAG AGGCCACCCT GCCGAGGTGG
CGGGCGGGTG AGCAGGCCCA GCCCCCAGGG GACCAGGAGC CTGAGGCTGT GCTGGCCATG
CTCAGGTCCC TAGACCTGGA GGGCCTGCGG AGCACGCTGG CCGAGAGCAG CGACCACGTG
GAAAAGAGTC CCCAGTCCCT CCTGCAGGAC ATGCTGGCCA CGGGAGGCTT CCTGCAGGGG
GACGAGGCCG ACTGCTACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001337556.1
CDS666..2606
Misc Feature(1)408..410
Exon (1)1..272
Exon (2)273..415
Exon (3)416..570
Exon (4)571..943
Exon (5)944..1063
Exon (6)1064..1155
Exon (7)1156..1274
Exon (8)1275..1461
Exon (9)1462..1535
Exon (10)1536..1638
Exon (11)1639..1737
Exon (12)1738..1910
Exon (13)1911..2865
Translation

Target ORF information:

RefSeq Version NM_001350627.1
Organism Homo sapiens(Human)
Definition Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001350627.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGCGAGGTG GAGCCGAGGG GCAGCCCTGC CTGCCGGGGG GTGACTGGCC CGCGTGTGCC 
CAGAAGATCA TGGATCACGT TGAAGAGTCC TTGTGCTCCG CGGCCACCCC CAAGGTCACT
CAGGCCCTGA ACGTCCTGAC CACGACCTTC GGGCGCTGCC AGAGCCCCTG GACGGAAGCC
CTGTGGGTGC GGCTGAGTCC CCGCGTGGCC TGTCTGCTGG AGAGAGACCC CATCCCCGCC
GCACACTCGT TCGTGGACCT GCTTCTCTGT GTGGCTCGTT CTCCCGTGTT CAGTTCTTCC
GACGGCAGCC TGTGGGAGAC AGTGGCGCGG GCTCTGAGCT GCCTGGGTCC CACCCACATG
GGACCCCTGG CTTTGGGGAT CCTGAAGCTC GAGCACTGTC CACAGGCACT GAGGACCCAG
GCCTTCCAGG TCCTTCTCCA GCCCCTGGCC TGTGTCCTGA AGGCCACGGT TCAGGCCCCC
GGACCCCCAG GCTTGCTGGA CGGGACGGCA GACGATGCCA CGACGGTGGA CACACTCCTG
GCCTCCAAGT CGTCCTGCGC CGGCCTCCTG TGCCGCACCC TGGCTCACCT GGAGGAGCTG
CAGCCGCTGC CCCAGCGCCC TTCACCGTGG CCCCAGGCGT CTCTACTGGG GGCTACAGTG
ACTGTCCTGC GGCTCTGTGA CGGCTCGGCT GCCCCTGCCT CCAGTGTGGG GGGCCACCTC
TGTGGGACCC TGGCGGGCTG CGTCCGGGTC CAGCGAGCAG CCCTCGACTT CCTGGGGACG
CTGTCACAGG GGACAGGCCC CCAGGAGCTG GTGACGCAGG CGCTTGCTGT CCTCCTGGAG
TGCCTCGAGA GCCCCGGCTC CAGCCCCACG GTTCTGAAGA AGGCCTTCCA GGCCACGCTC
AGGTGGCTCC TGAGCTCACC CAAGACCCCC GGCTGCTCTG ATCTCGGCCC CCTCATCCCG
CAGTTCCTCA GAGAGCTGTT CCCTGTGCTG CAGAAACGCC TGTGCCACCC CTGCTGGGAG
GTGAGGGACT CCGCCCTCGA GTTCCTGACC CAGCTGAGCA GGCACTGGGG AGGACAGGCT
GACTTCAGAT GCGCACTCTT GGCTTCAGAG GTGCCTCAGC TGGCCCTGCA GCTCCTCCAG
GACCCTGAGA GTTATGTCCG AGCGAGTGCA GTGACCGCCA TGGGGCAGCT GTCCAGCCAG
GGCCTGCACG CCCCCACCAG CCCTGAGCAT GCAGAGGCCC GGCAGAGCCT GTTCCTGGAG
CTCCTGCACA TCCTCTCCGT AGACTCGGAG GGCTTCCCAC GGCGGGCGGT CATGCAAGTC
TTCACTGAGT GGCTGCGGGA CGGCCACGCC GACGCGGCCC AGGACACGGA GCAGTTCGTG
GCCACTGTGC TGCAGGCGGC GAGCCGAGAC CTGGACTGGG AGGTCCGCGC CCAGGGCCTG
GAGCTGGCCC TCGTGTTCCT GGGCCAGACT TTGGGGCCGC CGCGTACCCA CTGCCCCTAT
GCCGTGGCCC TACCCGAGGT GGCCCCAGCC CAGCCACTCA CCGAGGCACT GAGGGCTCTC
TGCCACGTGG GGCTCTTTGA CTTCGCCTTT TGTGCCTTGT TTGACTGCGA CCGCCCTGTG
GCGCAGAAGT CTTGTGACCT CCTTCTCTTC CTGAGGGACA AGATTGCTTC CTACAGCAGC
CTGCGGGAGG CCAGGGGCAG CCCCAACACT GCCTCCGCAG AGGCCACCCT GCCGAGGTGG
CGGGCGGGTG AGCAGGCCCA GCCCCCAGGG GACCAGGAGC CTGAGGCTGT GCTGGCCATG
CTCAGGTCCC TAGACCTGGA GGGCCTGCGG AGCACGCTGG CCGAGAGCAG CGACCACGTG
GAAAAGAGTC CCCAGTCCCT CCTGCAGGAC ATGCTGGCCA CGGGAGGCTT CCTGCAGGGG
GACGAGGCCG ACTGCTACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22650
Clone ID Related Accession (Same CDS sequence) NM_152743.3
Accession Version NM_152743.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2466bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-20
Organism Homo sapiens(Human)
Product BRCA1-associated ATM activator 1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092488.3, BP363179.1, BC015632.2 and AI963076.1. This sequence is a reference standard in the RefSeqGene project. On Aug 26, 2010 this sequence version replaced NM_152743.2. Transcript Variant: This variant (2) uses an alternate in-frame splice junction in the 3' end of the coding sequence compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC015632.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGACCCAG AATGCGCCCA GCTGCTCCCG GCTCTCTGTG CTGTTCTGGT AGATCCCAGG 
CAGCCGGTGG CAGATGACAC CTGTTTGGAG AAGCTCCTGG ACTGGTTTAA AACGGTCACT
GAAGGAGAGT CCAGTGTCGT GCTGCTGCAG GAGCACCCCT GCCTGGTGGA GCTGCTGTCC
CATGTGCTGA AAGTCCAGGA CCTGAGTTCT GGGGTCCTCT CCTTCTCACT GCGCCTGGCA
GGAACCTTCG CAGCCCAGGA AAACTGCTTC CAGTATCTTC AGCAGGGGGA GTTACTACCA
GGGCTCTTTG GGGAGCCAGG ACCCCTCGGC CGAGCAACCT GGGCCGTCCC CACCGTGCGC
AGCGGCTGGA TCCAGGGCCT GCGCTCCCTG GCACAGCACC CCAGCGCCCT GCGCTTCCTG
GCCGACCATG GTGCGGTCGA CACCATCTTC TCCCTGCAGG GAGACTCCAG CCTGTTTGTG
GCCTCGGCGG CCAGTCAGCT CCTGGTGCAC GTCCTGGCTT TGTCCATGCG AGGTGGAGCC
GAGGGGCAGC CCTGCCTGCC GGGGGGTGAC TGGCCCGCGT GTGCCCAGAA GATCATGGAT
CACGTTGAAG AGTCCTTGTG CTCCGCGGCC ACCCCCAAGG TCACTCAGGC CCTGAACGTC
CTGACCACGA CCTTCGGGCG CTGCCAGAGC CCCTGGACGG AAGCCCTGTG GGTGCGGCTG
AGTCCCCGCG TGGCCTGTCT GCTGGAGAGA GACCCCATCC CCGCCGCACA CTCGTTCGTG
GACCTGCTTC TCTGTGTGGC TCGTTCTCCC GTGTTCAGTT CTTCCGACGG CAGCCTGTGG
GAGACAGTGG CGCGGGCTCT GAGCTGCCTG GGTCCCACCC ACATGGGACC CCTGGCTTTG
GGGATCCTGA AGCTCGAGCA CTGTCCACAG GCACTGAGGA CCCAGGCCTT CCAGGTCCTT
CTCCAGCCCC TGGCCTGTGT CCTGAAGGCC ACGGTTCAGG CCCCCGGACC CCCAGGCTTG
CTGGACGGGA CGGCAGACGA TGCCACGACG GTGGACACAC TCCTGGCCTC CAAGTCGTCC
TGCGCCGGCC TCCTGTGCCG CACCCTGGCT CACCTGGAGG AGCTGCAGCC GCTGCCCCAG
CGCCCTTCAC CGTGGCCCCA GGCGTCTCTA CTGGGGGCTA CAGTGACTGT CCTGCGGCTC
TGTGACGGCT CGGCTGCCCC TGCCTCCAGT GTGGGGGGCC ACCTCTGTGG GACCCTGGCG
GGCTGCGTCC GGGTCCAGCG AGCAGCCCTC GACTTCCTGG GGACGCTGTC ACAGGGGACA
GGCCCCCAGG AGCTGGTGAC GCAGGCGCTT GCTGTCCTCC TGGAGTGCCT CGAGAGCCCC
GGCTCCAGCC CCACGGTTCT GAAGAAGGCC TTCCAGGCCA CGCTCAGGTG GCTCCTGAGC
TCACCCAAGA CCCCCGGCTG CTCTGATCTC GGCCCCCTCA TCCCGCAGTT CCTCAGAGAG
CTGTTCCCTG TGCTGCAGAA ACGCCTGTGC CACCCCTGCT GGGAGGTGAG GGACTCCGCC
CTCGAGTTCC TGACCCAGCT GAGCAGGCAC TGGGGAGGAC AGGCTGACTT CAGATGCGCA
CTCTTGGCTT CAGAGGTGCC TCAGCTGGCC CTGCAGCTCC TCCAGGACCC TGAGAGTTAT
GTCCGAGCGA GTGCAGTGAC CGCCATGGGG CAGCTGTCCA GCCAGGGCCT GCACGCCCCC
ACCAGCCCTG AGCATGCAGA GGCCCGGCAG AGCCTGTTCC TGGAGCTCCT GCACATCCTC
TCCGTAGACT CGGAGGGCTT CCCACGGCGG GCGGTCATGC AAGTCTTCAC TGAGTGGCTG
CGGGACGGCC ACGCCGACGC GGCCCAGGAC ACGGAGCAGT TCGTGGCCAC TGTGCTGCAG
GCGGCGAGCC GAGACCTGGA CTGGGAGGTC CGCGCCCAGG GCCTGGAGCT GGCCCTCGTG
TTCCTGGGCC AGACTTTGGG GCCGCCGCGT ACCCACTGCC CCTATGCCGT GGCCCTACCC
GAGGTGGCCC CAGCCCAGCC ACTCACCGAG GCACTGAGGG CTCTCTGCCA CGTGGGGCTC
TTTGACTTCG CCTTTTGTGC CTTGTTTGAC TGCGACCGCC CTGTGGCGCA GAAGTCTTGT
GACCTCCTTC TCTTCCTGAG GGACAAGATT GCTTCCTACA GCAGCCTGCG GGAGGCCAGG
GGCAGCCCCA ACACTGCCTC CGCAGAGGCC ACCCTGCCGA GGTGGCGGGC GGGTGAGCAG
GCCCAGCCCC CAGGGGACCA GGAGCCTGAG GCTGTGCTGG CCATGCTCAG GTCCCTAGAC
CTGGAGGGCC TGCGGAGCAC GCTGGCCGAG AGCAGCGACC ACGTGGAAAA GAGTCCCCAG
TCCCTCCTGC AGGACATGCT GGCCACGGGA GGCTTCCTGC AGGGGGACGA GGCCGACTGC
TACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_689956.2
CDS289..2754
Misc Feature(1)16..18
Misc Feature(2)586..888
Misc Feature(3)order(1567..1572,1579..1581,1588..1593,1690..1695,1702..1704,1744..1749,1756..1758,1840..1845,1852..1854,1861..1866,1891..1893,1975..1980,1987..1989,1996..2001)
Misc Feature(4)1633..1758
Misc Feature(5)1771..1881
Misc Feature(6)1786..1896
Misc Feature(7)1918..2016
Misc Feature(8)1921..2001
Misc Feature(9)2173..2256
Misc Feature(10)2512..2514
Exon (1)1..272
Exon (2)273..415
Exon (3)416..570
Exon (4)571..718
Exon (5)719..1091
Exon (6)1092..1211
Exon (7)1212..1303
Exon (8)1304..1422
Exon (9)1423..1609
Exon (10)1610..1683
Exon (11)1684..1786
Exon (12)1787..1885
Exon (13)1886..2058
Exon (14)2059..3013
Translation

Target ORF information:

RefSeq Version NM_152743.3
Organism Homo sapiens(Human)
Definition Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_152743.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGACCCAG AATGCGCCCA GCTGCTCCCG GCTCTCTGTG CTGTTCTGGT AGATCCCAGG 
CAGCCGGTGG CAGATGACAC CTGTTTGGAG AAGCTCCTGG ACTGGTTTAA AACGGTCACT
GAAGGAGAGT CCAGTGTCGT GCTGCTGCAG GAGCACCCCT GCCTGGTGGA GCTGCTGTCC
CATGTGCTGA AAGTCCAGGA CCTGAGTTCT GGGGTCCTCT CCTTCTCACT GCGCCTGGCA
GGAACCTTCG CAGCCCAGGA AAACTGCTTC CAGTATCTTC AGCAGGGGGA GTTACTACCA
GGGCTCTTTG GGGAGCCAGG ACCCCTCGGC CGAGCAACCT GGGCCGTCCC CACCGTGCGC
AGCGGCTGGA TCCAGGGCCT GCGCTCCCTG GCACAGCACC CCAGCGCCCT GCGCTTCCTG
GCCGACCATG GTGCGGTCGA CACCATCTTC TCCCTGCAGG GAGACTCCAG CCTGTTTGTG
GCCTCGGCGG CCAGTCAGCT CCTGGTGCAC GTCCTGGCTT TGTCCATGCG AGGTGGAGCC
GAGGGGCAGC CCTGCCTGCC GGGGGGTGAC TGGCCCGCGT GTGCCCAGAA GATCATGGAT
CACGTTGAAG AGTCCTTGTG CTCCGCGGCC ACCCCCAAGG TCACTCAGGC CCTGAACGTC
CTGACCACGA CCTTCGGGCG CTGCCAGAGC CCCTGGACGG AAGCCCTGTG GGTGCGGCTG
AGTCCCCGCG TGGCCTGTCT GCTGGAGAGA GACCCCATCC CCGCCGCACA CTCGTTCGTG
GACCTGCTTC TCTGTGTGGC TCGTTCTCCC GTGTTCAGTT CTTCCGACGG CAGCCTGTGG
GAGACAGTGG CGCGGGCTCT GAGCTGCCTG GGTCCCACCC ACATGGGACC CCTGGCTTTG
GGGATCCTGA AGCTCGAGCA CTGTCCACAG GCACTGAGGA CCCAGGCCTT CCAGGTCCTT
CTCCAGCCCC TGGCCTGTGT CCTGAAGGCC ACGGTTCAGG CCCCCGGACC CCCAGGCTTG
CTGGACGGGA CGGCAGACGA TGCCACGACG GTGGACACAC TCCTGGCCTC CAAGTCGTCC
TGCGCCGGCC TCCTGTGCCG CACCCTGGCT CACCTGGAGG AGCTGCAGCC GCTGCCCCAG
CGCCCTTCAC CGTGGCCCCA GGCGTCTCTA CTGGGGGCTA CAGTGACTGT CCTGCGGCTC
TGTGACGGCT CGGCTGCCCC TGCCTCCAGT GTGGGGGGCC ACCTCTGTGG GACCCTGGCG
GGCTGCGTCC GGGTCCAGCG AGCAGCCCTC GACTTCCTGG GGACGCTGTC ACAGGGGACA
GGCCCCCAGG AGCTGGTGAC GCAGGCGCTT GCTGTCCTCC TGGAGTGCCT CGAGAGCCCC
GGCTCCAGCC CCACGGTTCT GAAGAAGGCC TTCCAGGCCA CGCTCAGGTG GCTCCTGAGC
TCACCCAAGA CCCCCGGCTG CTCTGATCTC GGCCCCCTCA TCCCGCAGTT CCTCAGAGAG
CTGTTCCCTG TGCTGCAGAA ACGCCTGTGC CACCCCTGCT GGGAGGTGAG GGACTCCGCC
CTCGAGTTCC TGACCCAGCT GAGCAGGCAC TGGGGAGGAC AGGCTGACTT CAGATGCGCA
CTCTTGGCTT CAGAGGTGCC TCAGCTGGCC CTGCAGCTCC TCCAGGACCC TGAGAGTTAT
GTCCGAGCGA GTGCAGTGAC CGCCATGGGG CAGCTGTCCA GCCAGGGCCT GCACGCCCCC
ACCAGCCCTG AGCATGCAGA GGCCCGGCAG AGCCTGTTCC TGGAGCTCCT GCACATCCTC
TCCGTAGACT CGGAGGGCTT CCCACGGCGG GCGGTCATGC AAGTCTTCAC TGAGTGGCTG
CGGGACGGCC ACGCCGACGC GGCCCAGGAC ACGGAGCAGT TCGTGGCCAC TGTGCTGCAG
GCGGCGAGCC GAGACCTGGA CTGGGAGGTC CGCGCCCAGG GCCTGGAGCT GGCCCTCGTG
TTCCTGGGCC AGACTTTGGG GCCGCCGCGT ACCCACTGCC CCTATGCCGT GGCCCTACCC
GAGGTGGCCC CAGCCCAGCC ACTCACCGAG GCACTGAGGG CTCTCTGCCA CGTGGGGCTC
TTTGACTTCG CCTTTTGTGC CTTGTTTGAC TGCGACCGCC CTGTGGCGCA GAAGTCTTGT
GACCTCCTTC TCTTCCTGAG GGACAAGATT GCTTCCTACA GCAGCCTGCG GGAGGCCAGG
GGCAGCCCCA ACACTGCCTC CGCAGAGGCC ACCCTGCCGA GGTGGCGGGC GGGTGAGCAG
GCCCAGCCCC CAGGGGACCA GGAGCCTGAG GCTGTGCTGG CCATGCTCAG GTCCCTAGAC
CTGGAGGGCC TGCGGAGCAC GCTGGCCGAG AGCAGCGACC ACGTGGAAAA GAGTCCCCAG
TCCCTCCTGC AGGACATGCT GGCCACGGGA GGCTTCCTGC AGGGGGACGA GGCCGACTGC
TACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu41095
Clone ID Related Accession (Same CDS sequence) NM_001350626.1
Accession Version NM_001350626.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2646bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-20
Organism Homo sapiens(Human)
Product BRCA1-associated ATM activator 1 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092488.3. On Apr 20, 2017 this sequence version replaced XM_005249643.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.54500.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGGACCCAG AATGCGCCCA GCTGCTCCCG GCTCTCTGTG CTGTTCTGGT AGATCCCAGG 
CAGCCGGTGG CAGATGACAC CTGTTTGGAG AAGCTCCTGG ACTGGTTTAA AACGGTCACT
GAAGGAGAGT CCAGTGTCGT GCTGCTGCAG GAGCACCCCT GCCTGGTGGA GCTGCTGTCC
CATGTGCTGA AAGTCCAGGA CCTGAGTTCT GGGGTCCTCT CCTTCTCACT GCGCCTGGCA
GGAACCTTCG CAGCCCAGGA AAACTGCTTC CAGTATCTTC AGCAGGGGGA GTTACTACCA
GGGCTCTTTG GGGAGCCAGG ACCCCTCGGC CGAGCAACCT GGGCCGTCCC CACCGTGCGC
AGCGGCTGGA TCCAGGGCCT GCGCTCCCTG GCACAGCACC CCAGCGCCCT GCGCTTCCTG
GCCGACCATG GTGCGGTCGA CACCATCTTC TCCCTGCAGG GAGACTCCAG CCTGTTTGTG
GCCTCGGCGG CCAGTCAGCT CCTGGTGCAC GTCCTGGCTT TGTCCATGCG AGGTGGAGCC
GAGGGGCAGC CCTGCCTGCC GGGGGGTGAC TGGCCCGCGT GTGCCCAGAA GATCATGGAT
CACGTTGAAG AGTCCTTGTG CTCCGCGGCC ACCCCCAAGG TCACTCAGGC CCTGAACGTC
CTGACCACGA CCTTCGGGCG CTGCCAGAGC CCCTGGACGG AAGCCCTGTG GGTGCGGCTG
AGTCCCCGCG TGGCCTGTCT GCTGGAGAGA GACCCCATCC CCGCCGCACA CTCGTTCGTG
GACCTGCTTC TCTGTGTGGC TCGTTCTCCC GTGTTCAGTT CTTCCGACGG CAGCCTGTGG
GAGACAGTGG CGCGGGCTCT GAGCTGCCTG GGTCCCACCC ACATGGGACC CCTGGCTTTG
GGGATCCTGA AGCTCGAGCA CTGTCCACAG GCACTGAGGA CCCAGGCCTT CCAGGTCCTT
CTCCAGCCCC TGGCCTGTGT CCTGAAGGCC ACGGTTCAGG CCCCCGGACC CCCAGGCTTG
CTGGACGGGA CGGCAGACGA TGCCACGACG GTGGACACAC TCCTGGCCTC CAAGTCGTCC
TGCGCCGGCC TCCTGTGCCG CACCCTGGCT CACCTGGAGG AGCTGCAGCC GCTGCCCCAG
CGCCCTTCAC CGTGGCCCCA GGCGTCTCTA CTGGGGGCTA CAGTGACTGT CCTGCGGCTC
TGTGACGGCT CGGCTGCCCC TGCCTCCAGT GTGGGGGGCC ACCTCTGTGG GACCCTGGCG
GGCTGCGTCC GGGTCCAGCG AGCAGCCCTC GACTTCCTGG GGACGCTGTC ACAGGGGACA
GGCCCCCAGG AGCTGGTGAC GCAGGCGCTT GCTGTCCTCC TGGAGTGCCT CGAGAGCCCC
GGCTCCAGCC CCACGGTTCT GAAGAAGGCC TTCCAGGCCA CGCTCAGGTG GCTCCTGAGC
TCACCCAAGA CCCCCGGCTG CTCTGATCTC GGCCCCCTCA TCCCGCAGTT CCTCAGAGAG
CTGTTCCCTG TGCTGCAGAA ACGCCTGTGC CACCCCTGCT GGGAGGTGAG GGACTCCGCC
CTCGAGTTCC TGACCCAGCT GAGCAGGCAC TGGGGAGGAC AGGCTGACTT CAGATGCGCA
CTCTTGGCTT CAGAGGTGCC TCAGCTGGCC CTGCAGCTCC TCCAGGACCC TGAGAGTTAT
GTCCGAGCGA GTGCAGTGAC CGCCATGGGG CAGCTGTCCA GCCAGGGCCT GCACGCCCCC
ACCAGCCCTG AGCATGCAGA GGCCCGGCAG ATGGGTAAAC TGAGGATTGG AGGGCCGTGT
GCTCACTGCG CAGCATGGGA AGGTGTCAGA GCGGGCTGTG GCCCTAGACT GCATGTCCGG
GGCCAGCCCC CATCATGTAC CGGAGTCCTG TTGCGGGAGC CTCGCTCCTG CCACCCCACC
AACCATCCGC ACTTGCTCCC TGTCCCCCAG AGCCTGTTCC TGGAGCTCCT GCACATCCTC
TCCGTAGACT CGGAGGGCTT CCCACGGCGG GCGGTCATGC AAGTCTTCAC TGAGTGGCTG
CGGGACGGCC ACGCCGACGC GGCCCAGGAC ACGGAGCAGT TCGTGGCCAC TGTGCTGCAG
GCGGCGAGCC GAGACCTGGA CTGGGAGGTC CGCGCCCAGG GCCTGGAGCT GGCCCTCGTG
TTCCTGGGCC AGACTTTGGG GCCGCCGCGT ACCCACTGCC CCTATGCCGT GGCCCTACCC
GAGGTGGCCC CAGCCCAGCC ACTCACCGAG GCACTGAGGG CTCTCTGCCA CGTGGGGCTC
TTTGACTTCG CCTTTTGTGC CTTGTTTGAC TGCGACCGCC CTGTGGCGCA GAAGTCTTGT
GACCTCCTTC TCTTCCTGAG GGACAAGATT GCTTCCTACA GCAGCCTGCG GGAGGCCAGG
GGCAGCCCCA ACACTGCCTC CGCAGAGGCC ACCCTGCCGA GGTGGCGGGC GGGTGAGCAG
GCCCAGCCCC CAGGGGACCA GGAGCCTGAG GCTGTGCTGG CCATGCTCAG GTCCCTAGAC
CTGGAGGGCC TGCGGAGCAC GCTGGCCGAG AGCAGCGACC ACGTGGAAAA GAGTCCCCAG
TCCCTCCTGC AGGACATGCT GGCCACGGGA GGCTTCCTGC AGGGGGACGA GGCCGACTGC
TACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001337555.1
CDS289..2934
Misc Feature(1)16..18
Exon (1)1..272
Exon (2)273..415
Exon (3)416..570
Exon (4)571..718
Exon (5)719..1091
Exon (6)1092..1211
Exon (7)1212..1303
Exon (8)1304..1422
Exon (9)1423..1609
Exon (10)1610..1683
Exon (11)1684..1786
Exon (12)1787..1885
Exon (13)1886..2058
Exon (14)2059..3193
Translation

Target ORF information:

RefSeq Version NM_001350626.1
Organism Homo sapiens(Human)
Definition Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001350626.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGGACCCAG AATGCGCCCA GCTGCTCCCG GCTCTCTGTG CTGTTCTGGT AGATCCCAGG 
CAGCCGGTGG CAGATGACAC CTGTTTGGAG AAGCTCCTGG ACTGGTTTAA AACGGTCACT
GAAGGAGAGT CCAGTGTCGT GCTGCTGCAG GAGCACCCCT GCCTGGTGGA GCTGCTGTCC
CATGTGCTGA AAGTCCAGGA CCTGAGTTCT GGGGTCCTCT CCTTCTCACT GCGCCTGGCA
GGAACCTTCG CAGCCCAGGA AAACTGCTTC CAGTATCTTC AGCAGGGGGA GTTACTACCA
GGGCTCTTTG GGGAGCCAGG ACCCCTCGGC CGAGCAACCT GGGCCGTCCC CACCGTGCGC
AGCGGCTGGA TCCAGGGCCT GCGCTCCCTG GCACAGCACC CCAGCGCCCT GCGCTTCCTG
GCCGACCATG GTGCGGTCGA CACCATCTTC TCCCTGCAGG GAGACTCCAG CCTGTTTGTG
GCCTCGGCGG CCAGTCAGCT CCTGGTGCAC GTCCTGGCTT TGTCCATGCG AGGTGGAGCC
GAGGGGCAGC CCTGCCTGCC GGGGGGTGAC TGGCCCGCGT GTGCCCAGAA GATCATGGAT
CACGTTGAAG AGTCCTTGTG CTCCGCGGCC ACCCCCAAGG TCACTCAGGC CCTGAACGTC
CTGACCACGA CCTTCGGGCG CTGCCAGAGC CCCTGGACGG AAGCCCTGTG GGTGCGGCTG
AGTCCCCGCG TGGCCTGTCT GCTGGAGAGA GACCCCATCC CCGCCGCACA CTCGTTCGTG
GACCTGCTTC TCTGTGTGGC TCGTTCTCCC GTGTTCAGTT CTTCCGACGG CAGCCTGTGG
GAGACAGTGG CGCGGGCTCT GAGCTGCCTG GGTCCCACCC ACATGGGACC CCTGGCTTTG
GGGATCCTGA AGCTCGAGCA CTGTCCACAG GCACTGAGGA CCCAGGCCTT CCAGGTCCTT
CTCCAGCCCC TGGCCTGTGT CCTGAAGGCC ACGGTTCAGG CCCCCGGACC CCCAGGCTTG
CTGGACGGGA CGGCAGACGA TGCCACGACG GTGGACACAC TCCTGGCCTC CAAGTCGTCC
TGCGCCGGCC TCCTGTGCCG CACCCTGGCT CACCTGGAGG AGCTGCAGCC GCTGCCCCAG
CGCCCTTCAC CGTGGCCCCA GGCGTCTCTA CTGGGGGCTA CAGTGACTGT CCTGCGGCTC
TGTGACGGCT CGGCTGCCCC TGCCTCCAGT GTGGGGGGCC ACCTCTGTGG GACCCTGGCG
GGCTGCGTCC GGGTCCAGCG AGCAGCCCTC GACTTCCTGG GGACGCTGTC ACAGGGGACA
GGCCCCCAGG AGCTGGTGAC GCAGGCGCTT GCTGTCCTCC TGGAGTGCCT CGAGAGCCCC
GGCTCCAGCC CCACGGTTCT GAAGAAGGCC TTCCAGGCCA CGCTCAGGTG GCTCCTGAGC
TCACCCAAGA CCCCCGGCTG CTCTGATCTC GGCCCCCTCA TCCCGCAGTT CCTCAGAGAG
CTGTTCCCTG TGCTGCAGAA ACGCCTGTGC CACCCCTGCT GGGAGGTGAG GGACTCCGCC
CTCGAGTTCC TGACCCAGCT GAGCAGGCAC TGGGGAGGAC AGGCTGACTT CAGATGCGCA
CTCTTGGCTT CAGAGGTGCC TCAGCTGGCC CTGCAGCTCC TCCAGGACCC TGAGAGTTAT
GTCCGAGCGA GTGCAGTGAC CGCCATGGGG CAGCTGTCCA GCCAGGGCCT GCACGCCCCC
ACCAGCCCTG AGCATGCAGA GGCCCGGCAG ATGGGTAAAC TGAGGATTGG AGGGCCGTGT
GCTCACTGCG CAGCATGGGA AGGTGTCAGA GCGGGCTGTG GCCCTAGACT GCATGTCCGG
GGCCAGCCCC CATCATGTAC CGGAGTCCTG TTGCGGGAGC CTCGCTCCTG CCACCCCACC
AACCATCCGC ACTTGCTCCC TGTCCCCCAG AGCCTGTTCC TGGAGCTCCT GCACATCCTC
TCCGTAGACT CGGAGGGCTT CCCACGGCGG GCGGTCATGC AAGTCTTCAC TGAGTGGCTG
CGGGACGGCC ACGCCGACGC GGCCCAGGAC ACGGAGCAGT TCGTGGCCAC TGTGCTGCAG
GCGGCGAGCC GAGACCTGGA CTGGGAGGTC CGCGCCCAGG GCCTGGAGCT GGCCCTCGTG
TTCCTGGGCC AGACTTTGGG GCCGCCGCGT ACCCACTGCC CCTATGCCGT GGCCCTACCC
GAGGTGGCCC CAGCCCAGCC ACTCACCGAG GCACTGAGGG CTCTCTGCCA CGTGGGGCTC
TTTGACTTCG CCTTTTGTGC CTTGTTTGAC TGCGACCGCC CTGTGGCGCA GAAGTCTTGT
GACCTCCTTC TCTTCCTGAG GGACAAGATT GCTTCCTACA GCAGCCTGCG GGAGGCCAGG
GGCAGCCCCA ACACTGCCTC CGCAGAGGCC ACCCTGCCGA GGTGGCGGGC GGGTGAGCAG
GCCCAGCCCC CAGGGGACCA GGAGCCTGAG GCTGTGCTGG CCATGCTCAG GTCCCTAGAC
CTGGAGGGCC TGCGGAGCAC GCTGGCCGAG AGCAGCGACC ACGTGGAAAA GAGTCCCCAG
TCCCTCCTGC AGGACATGCT GGCCACGGGA GGCTTCCTGC AGGGGGACGA GGCCGACTGC
TACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu78063
Clone ID Related Accession (Same CDS sequence) XM_011515178.1 , XM_011515182.2 , XM_011515177.2
Accession Version XM_011515178.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2730bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product BRCA1-associated ATM activator 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGACCCAG AATGCGCCCA GCTGCTCCCG GCTCTCTGTG CTGTTCTGGT AGATCCCAGG 
CAGCCGGTGG CAGATGACAC CTGTTTGGAG AAGCTCCTGG ACTGGTTTAA AACGGTCACT
GAAGGAGAGT CCAGTGTCGT GCTGCTGCAG GAGCACCCCT GCCTGGTGGA GCTGCTGTCC
CATGTGCTGA AAGTCCAGGA CCTGAGTTCT GGGGTCCTCT CCTTCTCACT GCGCCTGGCA
GGAACCTTCG CAGCCCAGGA AAACTGCTTC CAGTATCTTC AGCAGGGGGA GTTACTACCA
GGGCTCTTTG GGGAGCCAGG ACCCCTCGGC CGAGCAACCT GGGCCGTCCC CACCGTGCGC
AGCGGCTGGA TCCAGGGCCT GCGCTCCCTG GCACAGCACC CCAGCGCCCT GCGCTTCCTG
GCCGACCATG GTGCGGTCGA CACCATCTTC TCCCTGCAGG GAGACTCCAG CCTGTTTGTG
GCCTCGGCGG CCAGTCAGCT CCTGGTGCAC GTCCTGGCTT TGTCCATGCG AGGTGGAGCC
GAGGGGCAGC CCTGCCTGCC GGGGGGTGAC TGGCCCGCGT GTGCCCAGAA GATCATGGAT
CACGTTGAAG AGTCCTTGTG CTCCGCGGCC ACCCCCAAGG TCACTCAGGC CCTGAACGTC
CTGACCACGA CCTTCGGGCG CTGCCAGAGC CCCTGGACGG AAGCCCTGTG GGTGCGGCTG
AGTCCCCGCG TGGCCTGTCT GCTGGAGAGA GACCCCATCC CCGCCGCACA CTCGTTCGTG
GACCTGCTTC TCTGTGTGGC TCGTTCTCCC GTGTTCAGTT CTTCCGACGG CAGCCTGTGG
GAGACAGTGG CGCGGGCTCT GAGCTGCCTG GGTCCCACCC ACATGGGACC CCTGGCTTTG
GGGATCCTGA AGCTCGAGCA CTGTCCACAG GCACTGAGGA CCCAGGCCTT CCAGGTCCTT
CTCCAGCCCC TGGCCTGTGT CCTGAAGGCC ACGGTTCAGG CCCCCGGACC CCCAGGCTTG
CTGGACGGGA CGGCAGACGA TGCCACGACG GTGGACACAC TCCTGGCCTC CAAGTCGTCC
TGCGCCGGCC TCCTGTGCCG CACCCTGGCT CACCTGGAGG AGCTGCAGCC GCTGCCCCAG
CGCCCTTCAC CGTGGCCCCA GGCGTCTCTA CTGGGGGCTA CAGTGACTGT CCTGCGGCTC
TGTGACGGCT CGGCTGCCCC TGCCTCCAGT GTGGGGGGCC ACCTCTGTGG GACCCTGGCG
GGCTGCGTCC GGGTCCAGCG AGCAGCCCTC GACTTCCTGG GGACGCTGTC ACAGGGGACA
GGCCCCCAGG AGCTGGTGAC GCAGGCGCTT GCTGTCCTCC TGGAGTGCCT CGAGAGCCCC
GGCTCCAGCC CCACGGCCTC TGCGGATGGG GCCTTCACAC AGGGCCACAG AGCTGCTGCT
CAGAGAGCTT CACGATGCAG CCCACCCTAT CTGCTCCAGG TTCTGAAGAA GGCCTTCCAG
GCCACGCTCA GGTGGCTCCT GAGCTCACCC AAGACCCCCG GCTGCTCTGA TCTCGGCCCC
CTCATCCCGC AGTTCCTCAG AGAGCTGTTC CCTGTGCTGC AGAAACGCCT GTGCCACCCC
TGCTGGGAGG TGAGGGACTC CGCCCTCGAG TTCCTGACCC AGCTGAGCAG GCACTGGGGA
GGACAGGCTG ACTTCAGATG CGCACTCTTG GCTTCAGAGG TGCCTCAGCT GGCCCTGCAG
CTCCTCCAGG ACCCTGAGAG TTATGTCCGA GCGAGTGCAG TGACCGCCAT GGGGCAGCTG
TCCAGCCAGG GCCTGCACGC CCCCACCAGC CCTGAGCATG CAGAGGCCCG GCAGATGGGT
AAACTGAGGA TTGGAGGGCC GTGTGCTCAC TGCGCAGCAT GGGAAGGTGT CAGAGCGGGC
TGTGGCCCTA GACTGCATGT CCGGGGCCAG CCCCCATCAT GTACCGGAGT CCTGTTGCGG
GAGCCTCGCT CCTGCCACCC CACCAACCAT CCGCACTTGC TCCCTGTCCC CCAGAGCCTG
TTCCTGGAGC TCCTGCACAT CCTCTCCGTA GACTCGGAGG GCTTCCCACG GCGGGCGGTC
ATGCAAGTCT TCACTGAGTG GCTGCGGGAC GGCCACGCCG ACGCGGCCCA GGACACGGAG
CAGTTCGTGG CCACTGTGCT GCAGGCGGCG AGCCGAGACC TGGACTGGGA GGTCCGCGCC
CAGGGCCTGG AGCTGGCCCT CGTGTTCCTG GGCCAGACTT TGGGGCCGCC GCGTACCCAC
TGCCCCTATG CCGTGGCCCT ACCCGAGGTG GCCCCAGCCC AGCCACTCAC CGAGGCACTG
AGGGCTCTCT GCCACGTGGG GCTCTTTGAC TTCGCCTTTT GTGCCTTGTT TGACTGCGAC
CGCCCTGTGG CGCAGAAGTC TTGTGACCTC CTTCTCTTCC TGAGGGACAA GATTGCTTCC
TACAGCAGCC TGCGGGAGGC CAGGGGCAGC CCCAACACTG CCTCCGCAGA GGCCACCCTG
CCGAGGTGGC GGGCGGGTGA GCAGGCCCAG CCCCCAGGGG ACCAGGAGCC TGAGGCTGTG
CTGGCCATGC TCAGGTCCCT AGACCTGGAG GGCCTGCGGA GCACGCTGGC CGAGAGCAGC
GACCACGTGG AAAAGAGTCC CCAGTCCCTC CTGCAGGACA TGCTGGCCAC GGGAGGCTTC
CTGCAGGGGG ACGAGGCCGA CTGCTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011513480.1
CDS467..3196
Misc Feature(1)1814..1894
Misc Feature(2)order(1868..1873,1880..1882,1889..1894,1979..1984,1991..1993,2006..2011,2018..2020,2102..2107,2114..2116,2123..2128,2153..2155,2237..2242,2249..2251,2258..2263)
Misc Feature(3)1937..2020
Misc Feature(4)2048..2158
Misc Feature(5)2183..2263
Translation

Target ORF information:

RefSeq Version XM_011515178.1
Organism Homo sapiens(Human)
Definition Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011515178.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGACCCAG AATGCGCCCA GCTGCTCCCG GCTCTCTGTG CTGTTCTGGT AGATCCCAGG 
CAGCCGGTGG CAGATGACAC CTGTTTGGAG AAGCTCCTGG ACTGGTTTAA AACGGTCACT
GAAGGAGAGT CCAGTGTCGT GCTGCTGCAG GAGCACCCCT GCCTGGTGGA GCTGCTGTCC
CATGTGCTGA AAGTCCAGGA CCTGAGTTCT GGGGTCCTCT CCTTCTCACT GCGCCTGGCA
GGAACCTTCG CAGCCCAGGA AAACTGCTTC CAGTATCTTC AGCAGGGGGA GTTACTACCA
GGGCTCTTTG GGGAGCCAGG ACCCCTCGGC CGAGCAACCT GGGCCGTCCC CACCGTGCGC
AGCGGCTGGA TCCAGGGCCT GCGCTCCCTG GCACAGCACC CCAGCGCCCT GCGCTTCCTG
GCCGACCATG GTGCGGTCGA CACCATCTTC TCCCTGCAGG GAGACTCCAG CCTGTTTGTG
GCCTCGGCGG CCAGTCAGCT CCTGGTGCAC GTCCTGGCTT TGTCCATGCG AGGTGGAGCC
GAGGGGCAGC CCTGCCTGCC GGGGGGTGAC TGGCCCGCGT GTGCCCAGAA GATCATGGAT
CACGTTGAAG AGTCCTTGTG CTCCGCGGCC ACCCCCAAGG TCACTCAGGC CCTGAACGTC
CTGACCACGA CCTTCGGGCG CTGCCAGAGC CCCTGGACGG AAGCCCTGTG GGTGCGGCTG
AGTCCCCGCG TGGCCTGTCT GCTGGAGAGA GACCCCATCC CCGCCGCACA CTCGTTCGTG
GACCTGCTTC TCTGTGTGGC TCGTTCTCCC GTGTTCAGTT CTTCCGACGG CAGCCTGTGG
GAGACAGTGG CGCGGGCTCT GAGCTGCCTG GGTCCCACCC ACATGGGACC CCTGGCTTTG
GGGATCCTGA AGCTCGAGCA CTGTCCACAG GCACTGAGGA CCCAGGCCTT CCAGGTCCTT
CTCCAGCCCC TGGCCTGTGT CCTGAAGGCC ACGGTTCAGG CCCCCGGACC CCCAGGCTTG
CTGGACGGGA CGGCAGACGA TGCCACGACG GTGGACACAC TCCTGGCCTC CAAGTCGTCC
TGCGCCGGCC TCCTGTGCCG CACCCTGGCT CACCTGGAGG AGCTGCAGCC GCTGCCCCAG
CGCCCTTCAC CGTGGCCCCA GGCGTCTCTA CTGGGGGCTA CAGTGACTGT CCTGCGGCTC
TGTGACGGCT CGGCTGCCCC TGCCTCCAGT GTGGGGGGCC ACCTCTGTGG GACCCTGGCG
GGCTGCGTCC GGGTCCAGCG AGCAGCCCTC GACTTCCTGG GGACGCTGTC ACAGGGGACA
GGCCCCCAGG AGCTGGTGAC GCAGGCGCTT GCTGTCCTCC TGGAGTGCCT CGAGAGCCCC
GGCTCCAGCC CCACGGCCTC TGCGGATGGG GCCTTCACAC AGGGCCACAG AGCTGCTGCT
CAGAGAGCTT CACGATGCAG CCCACCCTAT CTGCTCCAGG TTCTGAAGAA GGCCTTCCAG
GCCACGCTCA GGTGGCTCCT GAGCTCACCC AAGACCCCCG GCTGCTCTGA TCTCGGCCCC
CTCATCCCGC AGTTCCTCAG AGAGCTGTTC CCTGTGCTGC AGAAACGCCT GTGCCACCCC
TGCTGGGAGG TGAGGGACTC CGCCCTCGAG TTCCTGACCC AGCTGAGCAG GCACTGGGGA
GGACAGGCTG ACTTCAGATG CGCACTCTTG GCTTCAGAGG TGCCTCAGCT GGCCCTGCAG
CTCCTCCAGG ACCCTGAGAG TTATGTCCGA GCGAGTGCAG TGACCGCCAT GGGGCAGCTG
TCCAGCCAGG GCCTGCACGC CCCCACCAGC CCTGAGCATG CAGAGGCCCG GCAGATGGGT
AAACTGAGGA TTGGAGGGCC GTGTGCTCAC TGCGCAGCAT GGGAAGGTGT CAGAGCGGGC
TGTGGCCCTA GACTGCATGT CCGGGGCCAG CCCCCATCAT GTACCGGAGT CCTGTTGCGG
GAGCCTCGCT CCTGCCACCC CACCAACCAT CCGCACTTGC TCCCTGTCCC CCAGAGCCTG
TTCCTGGAGC TCCTGCACAT CCTCTCCGTA GACTCGGAGG GCTTCCCACG GCGGGCGGTC
ATGCAAGTCT TCACTGAGTG GCTGCGGGAC GGCCACGCCG ACGCGGCCCA GGACACGGAG
CAGTTCGTGG CCACTGTGCT GCAGGCGGCG AGCCGAGACC TGGACTGGGA GGTCCGCGCC
CAGGGCCTGG AGCTGGCCCT CGTGTTCCTG GGCCAGACTT TGGGGCCGCC GCGTACCCAC
TGCCCCTATG CCGTGGCCCT ACCCGAGGTG GCCCCAGCCC AGCCACTCAC CGAGGCACTG
AGGGCTCTCT GCCACGTGGG GCTCTTTGAC TTCGCCTTTT GTGCCTTGTT TGACTGCGAC
CGCCCTGTGG CGCAGAAGTC TTGTGACCTC CTTCTCTTCC TGAGGGACAA GATTGCTTCC
TACAGCAGCC TGCGGGAGGC CAGGGGCAGC CCCAACACTG CCTCCGCAGA GGCCACCCTG
CCGAGGTGGC GGGCGGGTGA GCAGGCCCAG CCCCCAGGGG ACCAGGAGCC TGAGGCTGTG
CTGGCCATGC TCAGGTCCCT AGACCTGGAG GGCCTGCGGA GCACGCTGGC CGAGAGCAGC
GACCACGTGG AAAAGAGTCC CCAGTCCCTC CTGCAGGACA TGCTGGCCAC GGGAGGCTTC
CTGCAGGGGG ACGAGGCCGA CTGCTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu78063
Clone ID Related Accession (Same CDS sequence) XM_011515178.1 , XM_011515182.2 , XM_011515177.2
Accession Version XM_011515182.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2730bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product BRCA1-associated ATM activator 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515182.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGACCCAG AATGCGCCCA GCTGCTCCCG GCTCTCTGTG CTGTTCTGGT AGATCCCAGG 
CAGCCGGTGG CAGATGACAC CTGTTTGGAG AAGCTCCTGG ACTGGTTTAA AACGGTCACT
GAAGGAGAGT CCAGTGTCGT GCTGCTGCAG GAGCACCCCT GCCTGGTGGA GCTGCTGTCC
CATGTGCTGA AAGTCCAGGA CCTGAGTTCT GGGGTCCTCT CCTTCTCACT GCGCCTGGCA
GGAACCTTCG CAGCCCAGGA AAACTGCTTC CAGTATCTTC AGCAGGGGGA GTTACTACCA
GGGCTCTTTG GGGAGCCAGG ACCCCTCGGC CGAGCAACCT GGGCCGTCCC CACCGTGCGC
AGCGGCTGGA TCCAGGGCCT GCGCTCCCTG GCACAGCACC CCAGCGCCCT GCGCTTCCTG
GCCGACCATG GTGCGGTCGA CACCATCTTC TCCCTGCAGG GAGACTCCAG CCTGTTTGTG
GCCTCGGCGG CCAGTCAGCT CCTGGTGCAC GTCCTGGCTT TGTCCATGCG AGGTGGAGCC
GAGGGGCAGC CCTGCCTGCC GGGGGGTGAC TGGCCCGCGT GTGCCCAGAA GATCATGGAT
CACGTTGAAG AGTCCTTGTG CTCCGCGGCC ACCCCCAAGG TCACTCAGGC CCTGAACGTC
CTGACCACGA CCTTCGGGCG CTGCCAGAGC CCCTGGACGG AAGCCCTGTG GGTGCGGCTG
AGTCCCCGCG TGGCCTGTCT GCTGGAGAGA GACCCCATCC CCGCCGCACA CTCGTTCGTG
GACCTGCTTC TCTGTGTGGC TCGTTCTCCC GTGTTCAGTT CTTCCGACGG CAGCCTGTGG
GAGACAGTGG CGCGGGCTCT GAGCTGCCTG GGTCCCACCC ACATGGGACC CCTGGCTTTG
GGGATCCTGA AGCTCGAGCA CTGTCCACAG GCACTGAGGA CCCAGGCCTT CCAGGTCCTT
CTCCAGCCCC TGGCCTGTGT CCTGAAGGCC ACGGTTCAGG CCCCCGGACC CCCAGGCTTG
CTGGACGGGA CGGCAGACGA TGCCACGACG GTGGACACAC TCCTGGCCTC CAAGTCGTCC
TGCGCCGGCC TCCTGTGCCG CACCCTGGCT CACCTGGAGG AGCTGCAGCC GCTGCCCCAG
CGCCCTTCAC CGTGGCCCCA GGCGTCTCTA CTGGGGGCTA CAGTGACTGT CCTGCGGCTC
TGTGACGGCT CGGCTGCCCC TGCCTCCAGT GTGGGGGGCC ACCTCTGTGG GACCCTGGCG
GGCTGCGTCC GGGTCCAGCG AGCAGCCCTC GACTTCCTGG GGACGCTGTC ACAGGGGACA
GGCCCCCAGG AGCTGGTGAC GCAGGCGCTT GCTGTCCTCC TGGAGTGCCT CGAGAGCCCC
GGCTCCAGCC CCACGGCCTC TGCGGATGGG GCCTTCACAC AGGGCCACAG AGCTGCTGCT
CAGAGAGCTT CACGATGCAG CCCACCCTAT CTGCTCCAGG TTCTGAAGAA GGCCTTCCAG
GCCACGCTCA GGTGGCTCCT GAGCTCACCC AAGACCCCCG GCTGCTCTGA TCTCGGCCCC
CTCATCCCGC AGTTCCTCAG AGAGCTGTTC CCTGTGCTGC AGAAACGCCT GTGCCACCCC
TGCTGGGAGG TGAGGGACTC CGCCCTCGAG TTCCTGACCC AGCTGAGCAG GCACTGGGGA
GGACAGGCTG ACTTCAGATG CGCACTCTTG GCTTCAGAGG TGCCTCAGCT GGCCCTGCAG
CTCCTCCAGG ACCCTGAGAG TTATGTCCGA GCGAGTGCAG TGACCGCCAT GGGGCAGCTG
TCCAGCCAGG GCCTGCACGC CCCCACCAGC CCTGAGCATG CAGAGGCCCG GCAGATGGGT
AAACTGAGGA TTGGAGGGCC GTGTGCTCAC TGCGCAGCAT GGGAAGGTGT CAGAGCGGGC
TGTGGCCCTA GACTGCATGT CCGGGGCCAG CCCCCATCAT GTACCGGAGT CCTGTTGCGG
GAGCCTCGCT CCTGCCACCC CACCAACCAT CCGCACTTGC TCCCTGTCCC CCAGAGCCTG
TTCCTGGAGC TCCTGCACAT CCTCTCCGTA GACTCGGAGG GCTTCCCACG GCGGGCGGTC
ATGCAAGTCT TCACTGAGTG GCTGCGGGAC GGCCACGCCG ACGCGGCCCA GGACACGGAG
CAGTTCGTGG CCACTGTGCT GCAGGCGGCG AGCCGAGACC TGGACTGGGA GGTCCGCGCC
CAGGGCCTGG AGCTGGCCCT CGTGTTCCTG GGCCAGACTT TGGGGCCGCC GCGTACCCAC
TGCCCCTATG CCGTGGCCCT ACCCGAGGTG GCCCCAGCCC AGCCACTCAC CGAGGCACTG
AGGGCTCTCT GCCACGTGGG GCTCTTTGAC TTCGCCTTTT GTGCCTTGTT TGACTGCGAC
CGCCCTGTGG CGCAGAAGTC TTGTGACCTC CTTCTCTTCC TGAGGGACAA GATTGCTTCC
TACAGCAGCC TGCGGGAGGC CAGGGGCAGC CCCAACACTG CCTCCGCAGA GGCCACCCTG
CCGAGGTGGC GGGCGGGTGA GCAGGCCCAG CCCCCAGGGG ACCAGGAGCC TGAGGCTGTG
CTGGCCATGC TCAGGTCCCT AGACCTGGAG GGCCTGCGGA GCACGCTGGC CGAGAGCAGC
GACCACGTGG AAAAGAGTCC CCAGTCCCTC CTGCAGGACA TGCTGGCCAC GGGAGGCTTC
CTGCAGGGGG ACGAGGCCGA CTGCTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011513484.1
CDS42..2771
Misc Feature(1)1389..1469
Misc Feature(2)order(1443..1448,1455..1457,1464..1469,1554..1559,1566..1568,1581..1586,1593..1595,1677..1682,1689..1691,1698..1703,1728..1730,1812..1817,1824..1826,1833..1838)
Misc Feature(3)1512..1595
Misc Feature(4)1623..1733
Misc Feature(5)1758..1838
Translation

Target ORF information:

RefSeq Version XM_011515182.2
Organism Homo sapiens(Human)
Definition Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011515182.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGACCCAG AATGCGCCCA GCTGCTCCCG GCTCTCTGTG CTGTTCTGGT AGATCCCAGG 
CAGCCGGTGG CAGATGACAC CTGTTTGGAG AAGCTCCTGG ACTGGTTTAA AACGGTCACT
GAAGGAGAGT CCAGTGTCGT GCTGCTGCAG GAGCACCCCT GCCTGGTGGA GCTGCTGTCC
CATGTGCTGA AAGTCCAGGA CCTGAGTTCT GGGGTCCTCT CCTTCTCACT GCGCCTGGCA
GGAACCTTCG CAGCCCAGGA AAACTGCTTC CAGTATCTTC AGCAGGGGGA GTTACTACCA
GGGCTCTTTG GGGAGCCAGG ACCCCTCGGC CGAGCAACCT GGGCCGTCCC CACCGTGCGC
AGCGGCTGGA TCCAGGGCCT GCGCTCCCTG GCACAGCACC CCAGCGCCCT GCGCTTCCTG
GCCGACCATG GTGCGGTCGA CACCATCTTC TCCCTGCAGG GAGACTCCAG CCTGTTTGTG
GCCTCGGCGG CCAGTCAGCT CCTGGTGCAC GTCCTGGCTT TGTCCATGCG AGGTGGAGCC
GAGGGGCAGC CCTGCCTGCC GGGGGGTGAC TGGCCCGCGT GTGCCCAGAA GATCATGGAT
CACGTTGAAG AGTCCTTGTG CTCCGCGGCC ACCCCCAAGG TCACTCAGGC CCTGAACGTC
CTGACCACGA CCTTCGGGCG CTGCCAGAGC CCCTGGACGG AAGCCCTGTG GGTGCGGCTG
AGTCCCCGCG TGGCCTGTCT GCTGGAGAGA GACCCCATCC CCGCCGCACA CTCGTTCGTG
GACCTGCTTC TCTGTGTGGC TCGTTCTCCC GTGTTCAGTT CTTCCGACGG CAGCCTGTGG
GAGACAGTGG CGCGGGCTCT GAGCTGCCTG GGTCCCACCC ACATGGGACC CCTGGCTTTG
GGGATCCTGA AGCTCGAGCA CTGTCCACAG GCACTGAGGA CCCAGGCCTT CCAGGTCCTT
CTCCAGCCCC TGGCCTGTGT CCTGAAGGCC ACGGTTCAGG CCCCCGGACC CCCAGGCTTG
CTGGACGGGA CGGCAGACGA TGCCACGACG GTGGACACAC TCCTGGCCTC CAAGTCGTCC
TGCGCCGGCC TCCTGTGCCG CACCCTGGCT CACCTGGAGG AGCTGCAGCC GCTGCCCCAG
CGCCCTTCAC CGTGGCCCCA GGCGTCTCTA CTGGGGGCTA CAGTGACTGT CCTGCGGCTC
TGTGACGGCT CGGCTGCCCC TGCCTCCAGT GTGGGGGGCC ACCTCTGTGG GACCCTGGCG
GGCTGCGTCC GGGTCCAGCG AGCAGCCCTC GACTTCCTGG GGACGCTGTC ACAGGGGACA
GGCCCCCAGG AGCTGGTGAC GCAGGCGCTT GCTGTCCTCC TGGAGTGCCT CGAGAGCCCC
GGCTCCAGCC CCACGGCCTC TGCGGATGGG GCCTTCACAC AGGGCCACAG AGCTGCTGCT
CAGAGAGCTT CACGATGCAG CCCACCCTAT CTGCTCCAGG TTCTGAAGAA GGCCTTCCAG
GCCACGCTCA GGTGGCTCCT GAGCTCACCC AAGACCCCCG GCTGCTCTGA TCTCGGCCCC
CTCATCCCGC AGTTCCTCAG AGAGCTGTTC CCTGTGCTGC AGAAACGCCT GTGCCACCCC
TGCTGGGAGG TGAGGGACTC CGCCCTCGAG TTCCTGACCC AGCTGAGCAG GCACTGGGGA
GGACAGGCTG ACTTCAGATG CGCACTCTTG GCTTCAGAGG TGCCTCAGCT GGCCCTGCAG
CTCCTCCAGG ACCCTGAGAG TTATGTCCGA GCGAGTGCAG TGACCGCCAT GGGGCAGCTG
TCCAGCCAGG GCCTGCACGC CCCCACCAGC CCTGAGCATG CAGAGGCCCG GCAGATGGGT
AAACTGAGGA TTGGAGGGCC GTGTGCTCAC TGCGCAGCAT GGGAAGGTGT CAGAGCGGGC
TGTGGCCCTA GACTGCATGT CCGGGGCCAG CCCCCATCAT GTACCGGAGT CCTGTTGCGG
GAGCCTCGCT CCTGCCACCC CACCAACCAT CCGCACTTGC TCCCTGTCCC CCAGAGCCTG
TTCCTGGAGC TCCTGCACAT CCTCTCCGTA GACTCGGAGG GCTTCCCACG GCGGGCGGTC
ATGCAAGTCT TCACTGAGTG GCTGCGGGAC GGCCACGCCG ACGCGGCCCA GGACACGGAG
CAGTTCGTGG CCACTGTGCT GCAGGCGGCG AGCCGAGACC TGGACTGGGA GGTCCGCGCC
CAGGGCCTGG AGCTGGCCCT CGTGTTCCTG GGCCAGACTT TGGGGCCGCC GCGTACCCAC
TGCCCCTATG CCGTGGCCCT ACCCGAGGTG GCCCCAGCCC AGCCACTCAC CGAGGCACTG
AGGGCTCTCT GCCACGTGGG GCTCTTTGAC TTCGCCTTTT GTGCCTTGTT TGACTGCGAC
CGCCCTGTGG CGCAGAAGTC TTGTGACCTC CTTCTCTTCC TGAGGGACAA GATTGCTTCC
TACAGCAGCC TGCGGGAGGC CAGGGGCAGC CCCAACACTG CCTCCGCAGA GGCCACCCTG
CCGAGGTGGC GGGCGGGTGA GCAGGCCCAG CCCCCAGGGG ACCAGGAGCC TGAGGCTGTG
CTGGCCATGC TCAGGTCCCT AGACCTGGAG GGCCTGCGGA GCACGCTGGC CGAGAGCAGC
GACCACGTGG AAAAGAGTCC CCAGTCCCTC CTGCAGGACA TGCTGGCCAC GGGAGGCTTC
CTGCAGGGGG ACGAGGCCGA CTGCTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu78063
Clone ID Related Accession (Same CDS sequence) XM_011515178.1 , XM_011515182.2 , XM_011515177.2
Accession Version XM_011515177.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2730bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product BRCA1-associated ATM activator 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515177.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGACCCAG AATGCGCCCA GCTGCTCCCG GCTCTCTGTG CTGTTCTGGT AGATCCCAGG 
CAGCCGGTGG CAGATGACAC CTGTTTGGAG AAGCTCCTGG ACTGGTTTAA AACGGTCACT
GAAGGAGAGT CCAGTGTCGT GCTGCTGCAG GAGCACCCCT GCCTGGTGGA GCTGCTGTCC
CATGTGCTGA AAGTCCAGGA CCTGAGTTCT GGGGTCCTCT CCTTCTCACT GCGCCTGGCA
GGAACCTTCG CAGCCCAGGA AAACTGCTTC CAGTATCTTC AGCAGGGGGA GTTACTACCA
GGGCTCTTTG GGGAGCCAGG ACCCCTCGGC CGAGCAACCT GGGCCGTCCC CACCGTGCGC
AGCGGCTGGA TCCAGGGCCT GCGCTCCCTG GCACAGCACC CCAGCGCCCT GCGCTTCCTG
GCCGACCATG GTGCGGTCGA CACCATCTTC TCCCTGCAGG GAGACTCCAG CCTGTTTGTG
GCCTCGGCGG CCAGTCAGCT CCTGGTGCAC GTCCTGGCTT TGTCCATGCG AGGTGGAGCC
GAGGGGCAGC CCTGCCTGCC GGGGGGTGAC TGGCCCGCGT GTGCCCAGAA GATCATGGAT
CACGTTGAAG AGTCCTTGTG CTCCGCGGCC ACCCCCAAGG TCACTCAGGC CCTGAACGTC
CTGACCACGA CCTTCGGGCG CTGCCAGAGC CCCTGGACGG AAGCCCTGTG GGTGCGGCTG
AGTCCCCGCG TGGCCTGTCT GCTGGAGAGA GACCCCATCC CCGCCGCACA CTCGTTCGTG
GACCTGCTTC TCTGTGTGGC TCGTTCTCCC GTGTTCAGTT CTTCCGACGG CAGCCTGTGG
GAGACAGTGG CGCGGGCTCT GAGCTGCCTG GGTCCCACCC ACATGGGACC CCTGGCTTTG
GGGATCCTGA AGCTCGAGCA CTGTCCACAG GCACTGAGGA CCCAGGCCTT CCAGGTCCTT
CTCCAGCCCC TGGCCTGTGT CCTGAAGGCC ACGGTTCAGG CCCCCGGACC CCCAGGCTTG
CTGGACGGGA CGGCAGACGA TGCCACGACG GTGGACACAC TCCTGGCCTC CAAGTCGTCC
TGCGCCGGCC TCCTGTGCCG CACCCTGGCT CACCTGGAGG AGCTGCAGCC GCTGCCCCAG
CGCCCTTCAC CGTGGCCCCA GGCGTCTCTA CTGGGGGCTA CAGTGACTGT CCTGCGGCTC
TGTGACGGCT CGGCTGCCCC TGCCTCCAGT GTGGGGGGCC ACCTCTGTGG GACCCTGGCG
GGCTGCGTCC GGGTCCAGCG AGCAGCCCTC GACTTCCTGG GGACGCTGTC ACAGGGGACA
GGCCCCCAGG AGCTGGTGAC GCAGGCGCTT GCTGTCCTCC TGGAGTGCCT CGAGAGCCCC
GGCTCCAGCC CCACGGCCTC TGCGGATGGG GCCTTCACAC AGGGCCACAG AGCTGCTGCT
CAGAGAGCTT CACGATGCAG CCCACCCTAT CTGCTCCAGG TTCTGAAGAA GGCCTTCCAG
GCCACGCTCA GGTGGCTCCT GAGCTCACCC AAGACCCCCG GCTGCTCTGA TCTCGGCCCC
CTCATCCCGC AGTTCCTCAG AGAGCTGTTC CCTGTGCTGC AGAAACGCCT GTGCCACCCC
TGCTGGGAGG TGAGGGACTC CGCCCTCGAG TTCCTGACCC AGCTGAGCAG GCACTGGGGA
GGACAGGCTG ACTTCAGATG CGCACTCTTG GCTTCAGAGG TGCCTCAGCT GGCCCTGCAG
CTCCTCCAGG ACCCTGAGAG TTATGTCCGA GCGAGTGCAG TGACCGCCAT GGGGCAGCTG
TCCAGCCAGG GCCTGCACGC CCCCACCAGC CCTGAGCATG CAGAGGCCCG GCAGATGGGT
AAACTGAGGA TTGGAGGGCC GTGTGCTCAC TGCGCAGCAT GGGAAGGTGT CAGAGCGGGC
TGTGGCCCTA GACTGCATGT CCGGGGCCAG CCCCCATCAT GTACCGGAGT CCTGTTGCGG
GAGCCTCGCT CCTGCCACCC CACCAACCAT CCGCACTTGC TCCCTGTCCC CCAGAGCCTG
TTCCTGGAGC TCCTGCACAT CCTCTCCGTA GACTCGGAGG GCTTCCCACG GCGGGCGGTC
ATGCAAGTCT TCACTGAGTG GCTGCGGGAC GGCCACGCCG ACGCGGCCCA GGACACGGAG
CAGTTCGTGG CCACTGTGCT GCAGGCGGCG AGCCGAGACC TGGACTGGGA GGTCCGCGCC
CAGGGCCTGG AGCTGGCCCT CGTGTTCCTG GGCCAGACTT TGGGGCCGCC GCGTACCCAC
TGCCCCTATG CCGTGGCCCT ACCCGAGGTG GCCCCAGCCC AGCCACTCAC CGAGGCACTG
AGGGCTCTCT GCCACGTGGG GCTCTTTGAC TTCGCCTTTT GTGCCTTGTT TGACTGCGAC
CGCCCTGTGG CGCAGAAGTC TTGTGACCTC CTTCTCTTCC TGAGGGACAA GATTGCTTCC
TACAGCAGCC TGCGGGAGGC CAGGGGCAGC CCCAACACTG CCTCCGCAGA GGCCACCCTG
CCGAGGTGGC GGGCGGGTGA GCAGGCCCAG CCCCCAGGGG ACCAGGAGCC TGAGGCTGTG
CTGGCCATGC TCAGGTCCCT AGACCTGGAG GGCCTGCGGA GCACGCTGGC CGAGAGCAGC
GACCACGTGG AAAAGAGTCC CCAGTCCCTC CTGCAGGACA TGCTGGCCAC GGGAGGCTTC
CTGCAGGGGG ACGAGGCCGA CTGCTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011513479.1
CDS256..2985
Misc Feature(1)1603..1683
Misc Feature(2)order(1657..1662,1669..1671,1678..1683,1768..1773,1780..1782,1795..1800,1807..1809,1891..1896,1903..1905,1912..1917,1942..1944,2026..2031,2038..2040,2047..2052)
Misc Feature(3)1726..1809
Misc Feature(4)1837..1947
Misc Feature(5)1972..2052
Translation

Target ORF information:

RefSeq Version XM_011515177.2
Organism Homo sapiens(Human)
Definition Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011515177.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGACCCAG AATGCGCCCA GCTGCTCCCG GCTCTCTGTG CTGTTCTGGT AGATCCCAGG 
CAGCCGGTGG CAGATGACAC CTGTTTGGAG AAGCTCCTGG ACTGGTTTAA AACGGTCACT
GAAGGAGAGT CCAGTGTCGT GCTGCTGCAG GAGCACCCCT GCCTGGTGGA GCTGCTGTCC
CATGTGCTGA AAGTCCAGGA CCTGAGTTCT GGGGTCCTCT CCTTCTCACT GCGCCTGGCA
GGAACCTTCG CAGCCCAGGA AAACTGCTTC CAGTATCTTC AGCAGGGGGA GTTACTACCA
GGGCTCTTTG GGGAGCCAGG ACCCCTCGGC CGAGCAACCT GGGCCGTCCC CACCGTGCGC
AGCGGCTGGA TCCAGGGCCT GCGCTCCCTG GCACAGCACC CCAGCGCCCT GCGCTTCCTG
GCCGACCATG GTGCGGTCGA CACCATCTTC TCCCTGCAGG GAGACTCCAG CCTGTTTGTG
GCCTCGGCGG CCAGTCAGCT CCTGGTGCAC GTCCTGGCTT TGTCCATGCG AGGTGGAGCC
GAGGGGCAGC CCTGCCTGCC GGGGGGTGAC TGGCCCGCGT GTGCCCAGAA GATCATGGAT
CACGTTGAAG AGTCCTTGTG CTCCGCGGCC ACCCCCAAGG TCACTCAGGC CCTGAACGTC
CTGACCACGA CCTTCGGGCG CTGCCAGAGC CCCTGGACGG AAGCCCTGTG GGTGCGGCTG
AGTCCCCGCG TGGCCTGTCT GCTGGAGAGA GACCCCATCC CCGCCGCACA CTCGTTCGTG
GACCTGCTTC TCTGTGTGGC TCGTTCTCCC GTGTTCAGTT CTTCCGACGG CAGCCTGTGG
GAGACAGTGG CGCGGGCTCT GAGCTGCCTG GGTCCCACCC ACATGGGACC CCTGGCTTTG
GGGATCCTGA AGCTCGAGCA CTGTCCACAG GCACTGAGGA CCCAGGCCTT CCAGGTCCTT
CTCCAGCCCC TGGCCTGTGT CCTGAAGGCC ACGGTTCAGG CCCCCGGACC CCCAGGCTTG
CTGGACGGGA CGGCAGACGA TGCCACGACG GTGGACACAC TCCTGGCCTC CAAGTCGTCC
TGCGCCGGCC TCCTGTGCCG CACCCTGGCT CACCTGGAGG AGCTGCAGCC GCTGCCCCAG
CGCCCTTCAC CGTGGCCCCA GGCGTCTCTA CTGGGGGCTA CAGTGACTGT CCTGCGGCTC
TGTGACGGCT CGGCTGCCCC TGCCTCCAGT GTGGGGGGCC ACCTCTGTGG GACCCTGGCG
GGCTGCGTCC GGGTCCAGCG AGCAGCCCTC GACTTCCTGG GGACGCTGTC ACAGGGGACA
GGCCCCCAGG AGCTGGTGAC GCAGGCGCTT GCTGTCCTCC TGGAGTGCCT CGAGAGCCCC
GGCTCCAGCC CCACGGCCTC TGCGGATGGG GCCTTCACAC AGGGCCACAG AGCTGCTGCT
CAGAGAGCTT CACGATGCAG CCCACCCTAT CTGCTCCAGG TTCTGAAGAA GGCCTTCCAG
GCCACGCTCA GGTGGCTCCT GAGCTCACCC AAGACCCCCG GCTGCTCTGA TCTCGGCCCC
CTCATCCCGC AGTTCCTCAG AGAGCTGTTC CCTGTGCTGC AGAAACGCCT GTGCCACCCC
TGCTGGGAGG TGAGGGACTC CGCCCTCGAG TTCCTGACCC AGCTGAGCAG GCACTGGGGA
GGACAGGCTG ACTTCAGATG CGCACTCTTG GCTTCAGAGG TGCCTCAGCT GGCCCTGCAG
CTCCTCCAGG ACCCTGAGAG TTATGTCCGA GCGAGTGCAG TGACCGCCAT GGGGCAGCTG
TCCAGCCAGG GCCTGCACGC CCCCACCAGC CCTGAGCATG CAGAGGCCCG GCAGATGGGT
AAACTGAGGA TTGGAGGGCC GTGTGCTCAC TGCGCAGCAT GGGAAGGTGT CAGAGCGGGC
TGTGGCCCTA GACTGCATGT CCGGGGCCAG CCCCCATCAT GTACCGGAGT CCTGTTGCGG
GAGCCTCGCT CCTGCCACCC CACCAACCAT CCGCACTTGC TCCCTGTCCC CCAGAGCCTG
TTCCTGGAGC TCCTGCACAT CCTCTCCGTA GACTCGGAGG GCTTCCCACG GCGGGCGGTC
ATGCAAGTCT TCACTGAGTG GCTGCGGGAC GGCCACGCCG ACGCGGCCCA GGACACGGAG
CAGTTCGTGG CCACTGTGCT GCAGGCGGCG AGCCGAGACC TGGACTGGGA GGTCCGCGCC
CAGGGCCTGG AGCTGGCCCT CGTGTTCCTG GGCCAGACTT TGGGGCCGCC GCGTACCCAC
TGCCCCTATG CCGTGGCCCT ACCCGAGGTG GCCCCAGCCC AGCCACTCAC CGAGGCACTG
AGGGCTCTCT GCCACGTGGG GCTCTTTGAC TTCGCCTTTT GTGCCTTGTT TGACTGCGAC
CGCCCTGTGG CGCAGAAGTC TTGTGACCTC CTTCTCTTCC TGAGGGACAA GATTGCTTCC
TACAGCAGCC TGCGGGAGGC CAGGGGCAGC CCCAACACTG CCTCCGCAGA GGCCACCCTG
CCGAGGTGGC GGGCGGGTGA GCAGGCCCAG CCCCCAGGGG ACCAGGAGCC TGAGGCTGTG
CTGGCCATGC TCAGGTCCCT AGACCTGGAG GGCCTGCGGA GCACGCTGGC CGAGAGCAGC
GACCACGTGG AAAAGAGTCC CCAGTCCCTC CTGCAGGACA TGCTGGCCAC GGGAGGCTTC
CTGCAGGGGG ACGAGGCCGA CTGCTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu78064
Clone ID Related Accession (Same CDS sequence) XM_011515179.2
Accession Version XM_011515179.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2727bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product BRCA1-associated ATM activator 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515179.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGACCCAG AATGCGCCCA GCTGCTCCCG GCTCTCTGTG CTGTTCTGGT AGATCCCAGG 
CAGCCGGTGG CAGATGACAC CTGTTTGGAG AAGCTCCTGG ACTGGTTTAA AACGGTCACT
GAAGGAGAGT CCAGTGTCGT GCTGCTGCAG GAGCACCCCT GCCTGGTGGA GCTGCTGTCC
CATGTGCTGA AAGTCCAGGA CCTGAGTTCT GGGGTCCTCT CCTTCTCACT GCGCCTGGCA
GGAACCTTCG CAGCCCAGGA AAACTGCTTC CAGTATCTTC AGGGGGAGTT ACTACCAGGG
CTCTTTGGGG AGCCAGGACC CCTCGGCCGA GCAACCTGGG CCGTCCCCAC CGTGCGCAGC
GGCTGGATCC AGGGCCTGCG CTCCCTGGCA CAGCACCCCA GCGCCCTGCG CTTCCTGGCC
GACCATGGTG CGGTCGACAC CATCTTCTCC CTGCAGGGAG ACTCCAGCCT GTTTGTGGCC
TCGGCGGCCA GTCAGCTCCT GGTGCACGTC CTGGCTTTGT CCATGCGAGG TGGAGCCGAG
GGGCAGCCCT GCCTGCCGGG GGGTGACTGG CCCGCGTGTG CCCAGAAGAT CATGGATCAC
GTTGAAGAGT CCTTGTGCTC CGCGGCCACC CCCAAGGTCA CTCAGGCCCT GAACGTCCTG
ACCACGACCT TCGGGCGCTG CCAGAGCCCC TGGACGGAAG CCCTGTGGGT GCGGCTGAGT
CCCCGCGTGG CCTGTCTGCT GGAGAGAGAC CCCATCCCCG CCGCACACTC GTTCGTGGAC
CTGCTTCTCT GTGTGGCTCG TTCTCCCGTG TTCAGTTCTT CCGACGGCAG CCTGTGGGAG
ACAGTGGCGC GGGCTCTGAG CTGCCTGGGT CCCACCCACA TGGGACCCCT GGCTTTGGGG
ATCCTGAAGC TCGAGCACTG TCCACAGGCA CTGAGGACCC AGGCCTTCCA GGTCCTTCTC
CAGCCCCTGG CCTGTGTCCT GAAGGCCACG GTTCAGGCCC CCGGACCCCC AGGCTTGCTG
GACGGGACGG CAGACGATGC CACGACGGTG GACACACTCC TGGCCTCCAA GTCGTCCTGC
GCCGGCCTCC TGTGCCGCAC CCTGGCTCAC CTGGAGGAGC TGCAGCCGCT GCCCCAGCGC
CCTTCACCGT GGCCCCAGGC GTCTCTACTG GGGGCTACAG TGACTGTCCT GCGGCTCTGT
GACGGCTCGG CTGCCCCTGC CTCCAGTGTG GGGGGCCACC TCTGTGGGAC CCTGGCGGGC
TGCGTCCGGG TCCAGCGAGC AGCCCTCGAC TTCCTGGGGA CGCTGTCACA GGGGACAGGC
CCCCAGGAGC TGGTGACGCA GGCGCTTGCT GTCCTCCTGG AGTGCCTCGA GAGCCCCGGC
TCCAGCCCCA CGGCCTCTGC GGATGGGGCC TTCACACAGG GCCACAGAGC TGCTGCTCAG
AGAGCTTCAC GATGCAGCCC ACCCTATCTG CTCCAGGTTC TGAAGAAGGC CTTCCAGGCC
ACGCTCAGGT GGCTCCTGAG CTCACCCAAG ACCCCCGGCT GCTCTGATCT CGGCCCCCTC
ATCCCGCAGT TCCTCAGAGA GCTGTTCCCT GTGCTGCAGA AACGCCTGTG CCACCCCTGC
TGGGAGGTGA GGGACTCCGC CCTCGAGTTC CTGACCCAGC TGAGCAGGCA CTGGGGAGGA
CAGGCTGACT TCAGATGCGC ACTCTTGGCT TCAGAGGTGC CTCAGCTGGC CCTGCAGCTC
CTCCAGGACC CTGAGAGTTA TGTCCGAGCG AGTGCAGTGA CCGCCATGGG GCAGCTGTCC
AGCCAGGGCC TGCACGCCCC CACCAGCCCT GAGCATGCAG AGGCCCGGCA GATGGGTAAA
CTGAGGATTG GAGGGCCGTG TGCTCACTGC GCAGCATGGG AAGGTGTCAG AGCGGGCTGT
GGCCCTAGAC TGCATGTCCG GGGCCAGCCC CCATCATGTA CCGGAGTCCT GTTGCGGGAG
CCTCGCTCCT GCCACCCCAC CAACCATCCG CACTTGCTCC CTGTCCCCCA GAGCCTGTTC
CTGGAGCTCC TGCACATCCT CTCCGTAGAC TCGGAGGGCT TCCCACGGCG GGCGGTCATG
CAAGTCTTCA CTGAGTGGCT GCGGGACGGC CACGCCGACG CGGCCCAGGA CACGGAGCAG
TTCGTGGCCA CTGTGCTGCA GGCGGCGAGC CGAGACCTGG ACTGGGAGGT CCGCGCCCAG
GGCCTGGAGC TGGCCCTCGT GTTCCTGGGC CAGACTTTGG GGCCGCCGCG TACCCACTGC
CCCTATGCCG TGGCCCTACC CGAGGTGGCC CCAGCCCAGC CACTCACCGA GGCACTGAGG
GCTCTCTGCC ACGTGGGGCT CTTTGACTTC GCCTTTTGTG CCTTGTTTGA CTGCGACCGC
CCTGTGGCGC AGAAGTCTTG TGACCTCCTT CTCTTCCTGA GGGACAAGAT TGCTTCCTAC
AGCAGCCTGC GGGAGGCCAG GGGCAGCCCC AACACTGCCT CCGCAGAGGC CACCCTGCCG
AGGTGGCGGG CGGGTGAGCA GGCCCAGCCC CCAGGGGACC AGGAGCCTGA GGCTGTGCTG
GCCATGCTCA GGTCCCTAGA CCTGGAGGGC CTGCGGAGCA CGCTGGCCGA GAGCAGCGAC
CACGTGGAAA AGAGTCCCCA GTCCCTCCTG CAGGACATGC TGGCCACGGG AGGCTTCCTG
CAGGGGGACG AGGCCGACTG CTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011513481.1
CDS250..2976
Misc Feature(1)1594..1674
Misc Feature(2)order(1648..1653,1660..1662,1669..1674,1759..1764,1771..1773,1786..1791,1798..1800,1882..1887,1894..1896,1903..1908,1933..1935,2017..2022,2029..2031,2038..2043)
Misc Feature(3)1717..1800
Misc Feature(4)1828..1938
Misc Feature(5)1963..2043
Translation

Target ORF information:

RefSeq Version XM_011515179.2
Organism Homo sapiens(Human)
Definition Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011515179.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGACCCAG AATGCGCCCA GCTGCTCCCG GCTCTCTGTG CTGTTCTGGT AGATCCCAGG 
CAGCCGGTGG CAGATGACAC CTGTTTGGAG AAGCTCCTGG ACTGGTTTAA AACGGTCACT
GAAGGAGAGT CCAGTGTCGT GCTGCTGCAG GAGCACCCCT GCCTGGTGGA GCTGCTGTCC
CATGTGCTGA AAGTCCAGGA CCTGAGTTCT GGGGTCCTCT CCTTCTCACT GCGCCTGGCA
GGAACCTTCG CAGCCCAGGA AAACTGCTTC CAGTATCTTC AGGGGGAGTT ACTACCAGGG
CTCTTTGGGG AGCCAGGACC CCTCGGCCGA GCAACCTGGG CCGTCCCCAC CGTGCGCAGC
GGCTGGATCC AGGGCCTGCG CTCCCTGGCA CAGCACCCCA GCGCCCTGCG CTTCCTGGCC
GACCATGGTG CGGTCGACAC CATCTTCTCC CTGCAGGGAG ACTCCAGCCT GTTTGTGGCC
TCGGCGGCCA GTCAGCTCCT GGTGCACGTC CTGGCTTTGT CCATGCGAGG TGGAGCCGAG
GGGCAGCCCT GCCTGCCGGG GGGTGACTGG CCCGCGTGTG CCCAGAAGAT CATGGATCAC
GTTGAAGAGT CCTTGTGCTC CGCGGCCACC CCCAAGGTCA CTCAGGCCCT GAACGTCCTG
ACCACGACCT TCGGGCGCTG CCAGAGCCCC TGGACGGAAG CCCTGTGGGT GCGGCTGAGT
CCCCGCGTGG CCTGTCTGCT GGAGAGAGAC CCCATCCCCG CCGCACACTC GTTCGTGGAC
CTGCTTCTCT GTGTGGCTCG TTCTCCCGTG TTCAGTTCTT CCGACGGCAG CCTGTGGGAG
ACAGTGGCGC GGGCTCTGAG CTGCCTGGGT CCCACCCACA TGGGACCCCT GGCTTTGGGG
ATCCTGAAGC TCGAGCACTG TCCACAGGCA CTGAGGACCC AGGCCTTCCA GGTCCTTCTC
CAGCCCCTGG CCTGTGTCCT GAAGGCCACG GTTCAGGCCC CCGGACCCCC AGGCTTGCTG
GACGGGACGG CAGACGATGC CACGACGGTG GACACACTCC TGGCCTCCAA GTCGTCCTGC
GCCGGCCTCC TGTGCCGCAC CCTGGCTCAC CTGGAGGAGC TGCAGCCGCT GCCCCAGCGC
CCTTCACCGT GGCCCCAGGC GTCTCTACTG GGGGCTACAG TGACTGTCCT GCGGCTCTGT
GACGGCTCGG CTGCCCCTGC CTCCAGTGTG GGGGGCCACC TCTGTGGGAC CCTGGCGGGC
TGCGTCCGGG TCCAGCGAGC AGCCCTCGAC TTCCTGGGGA CGCTGTCACA GGGGACAGGC
CCCCAGGAGC TGGTGACGCA GGCGCTTGCT GTCCTCCTGG AGTGCCTCGA GAGCCCCGGC
TCCAGCCCCA CGGCCTCTGC GGATGGGGCC TTCACACAGG GCCACAGAGC TGCTGCTCAG
AGAGCTTCAC GATGCAGCCC ACCCTATCTG CTCCAGGTTC TGAAGAAGGC CTTCCAGGCC
ACGCTCAGGT GGCTCCTGAG CTCACCCAAG ACCCCCGGCT GCTCTGATCT CGGCCCCCTC
ATCCCGCAGT TCCTCAGAGA GCTGTTCCCT GTGCTGCAGA AACGCCTGTG CCACCCCTGC
TGGGAGGTGA GGGACTCCGC CCTCGAGTTC CTGACCCAGC TGAGCAGGCA CTGGGGAGGA
CAGGCTGACT TCAGATGCGC ACTCTTGGCT TCAGAGGTGC CTCAGCTGGC CCTGCAGCTC
CTCCAGGACC CTGAGAGTTA TGTCCGAGCG AGTGCAGTGA CCGCCATGGG GCAGCTGTCC
AGCCAGGGCC TGCACGCCCC CACCAGCCCT GAGCATGCAG AGGCCCGGCA GATGGGTAAA
CTGAGGATTG GAGGGCCGTG TGCTCACTGC GCAGCATGGG AAGGTGTCAG AGCGGGCTGT
GGCCCTAGAC TGCATGTCCG GGGCCAGCCC CCATCATGTA CCGGAGTCCT GTTGCGGGAG
CCTCGCTCCT GCCACCCCAC CAACCATCCG CACTTGCTCC CTGTCCCCCA GAGCCTGTTC
CTGGAGCTCC TGCACATCCT CTCCGTAGAC TCGGAGGGCT TCCCACGGCG GGCGGTCATG
CAAGTCTTCA CTGAGTGGCT GCGGGACGGC CACGCCGACG CGGCCCAGGA CACGGAGCAG
TTCGTGGCCA CTGTGCTGCA GGCGGCGAGC CGAGACCTGG ACTGGGAGGT CCGCGCCCAG
GGCCTGGAGC TGGCCCTCGT GTTCCTGGGC CAGACTTTGG GGCCGCCGCG TACCCACTGC
CCCTATGCCG TGGCCCTACC CGAGGTGGCC CCAGCCCAGC CACTCACCGA GGCACTGAGG
GCTCTCTGCC ACGTGGGGCT CTTTGACTTC GCCTTTTGTG CCTTGTTTGA CTGCGACCGC
CCTGTGGCGC AGAAGTCTTG TGACCTCCTT CTCTTCCTGA GGGACAAGAT TGCTTCCTAC
AGCAGCCTGC GGGAGGCCAG GGGCAGCCCC AACACTGCCT CCGCAGAGGC CACCCTGCCG
AGGTGGCGGG CGGGTGAGCA GGCCCAGCCC CCAGGGGACC AGGAGCCTGA GGCTGTGCTG
GCCATGCTCA GGTCCCTAGA CCTGGAGGGC CTGCGGAGCA CGCTGGCCGA GAGCAGCGAC
CACGTGGAAA AGAGTCCCCA GTCCCTCCTG CAGGACATGC TGGCCACGGG AGGCTTCCTG
CAGGGGGACG AGGCCGACTG CTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu78066
Clone ID Related Accession (Same CDS sequence) XM_011515181.2
Accession Version XM_011515181.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product BRCA1-associated ATM activator 1 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515181.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGGACCCAG AATGCGCCCA GCTGCTCCCG GCTCTCTGTG CTGTTCTGGT AGATCCCAGG 
CAGCCGGTGG CAGATGACAC CTGTTTGGAG AAGCTCCTGG ACTGGTTTAA AACGGTCACT
GAAGGAGAGT CCAGTGTCGT GCTGCTGCAG GAGCACCCCT GCCTGGTGGA GCTGCTGTCC
CATGTGCTGA AAGTCCAGGA CCTGAGTTCT GGGGTCCTCT CCTTCTCACT GCGCCTGGCA
GGAACCTTCG CAGCCCAGGA AAACTGCTTC CAGTATCTTC AGCAGGGGGA GTTACTACCA
GGGCTCTTTG GGGAGCCAGG ACCCCTCGGC CGAGCAACCT GGGCCGTCCC CACCGTGCGC
AGCGGCTGGA TCCAGGGCCT GCGCTCCCTG GCACAGCACC CCAGCGCCCT GCGCTTCCTG
GCCGACCATG GTGCGGTCGA CACCATCTTC TCCCTGCAGG GAGACTCCAG CCTGTTTGTG
GCCTCGGCGG CCAGTCAGCT CCTGGTGCAC GTCCTGGCTT TGTCCATGCG AGGTGGAGCC
GAGGGGCAGC CCTGCCTGCC GGGGGGTGAC TGGCCCGCGT GTGCCCAGAA GATCATGGAT
CACGTTGAAG AGTCCTTGTG CTCCGCGGCC ACCCCCAAGG TCACTCAGGC CCTGAACGTC
CTGACCACGA CCTTCGGGCG CTGCCAGAGC CCCTGGACGG AAGCCCTGTG GGTGCGGCTG
AGTCCCCGCG TGGCCTGTCT GCTGGAGAGA GACCCCATCC CCGCCGCACA CTCGTTCGTG
GACCTGCTTC TCTGTGTGGC TCGTTCTCCC GTGTTCAGTT CTTCCGACGG CAGCCTGTGG
GAGACAGTGG CGCGGGCTCT GAGCTGCCTG GGTCCCACCC ACATGGGACC CCTGGCTTTG
GGGATCCTGA AGCTCGAGCA CTGTCCACAG GCACTGAGGA CCCAGGCCTT CCAGGTCCTT
CTCCAGCCCC TGGCCTGTGT CCTGAAGGCC ACGGTTCAGG CCCCCGGACC CCCAGGCTTG
CTGGACGGGA CGGCAGACGA TGCCACGACG GTGGACACAC TCCTGGCCTC CAAGTCGTCC
TGCGCCGGCC TCCTGTGCCG CACCCTGGCT CACCTGGAGG AGCTGCAGCC GCTGCCCCAG
CGCCCTTCAC CGTGGCCCCA GGCGTCTCTA CTGGGGGCTA CAGTGACTGT CCTGCGGCTC
TGTGACGGCT CGGCTGCCCC TGCCTCCAGT GTGGGGGGCC ACCTCTGTGG GACCCTGGCG
GGCTGCGTCC GGGTCCAGCG AGCAGCCCTC GACTTCCTGG GGACGCTGTC ACAGGGGACA
GGCCCCCAGG AGCTGGTGAC GCAGGCGCTT GCTGTCCTCC TGGAGTGCCT CGAGAGCCCC
GGCTCCAGCC CCACGGCCTC TGCGGATGGG GCCTTCACAC AGGGCCACAG AGCTGCTGCT
CAGAGAGCTT CACGATGCAG CCCACCCTAT CTGCTCCAGG TTCTGAAGAA GGCCTTCCAG
GCCACGCTCA GGTGGCTCCT GAGCTCACCC AAGACCCCCG GCTGCTCTGA TCTCGGCCCC
CTCATCCCGC AGTTCCTCAG AGAGCTGTTC CCTGTGCTGC AGAAACGCCT GTGCCACCCC
TGCTGGGAGG TGAGGGACTC CGCCCTCGAG TTCCTGACCC AGCTGAGCAG GCACTGGGGA
GGACAGGCTG ACTTCAGATG CGCACTCTTG GCTTCAGAGG TGCCTCAGCT GGCCCTGCAG
CTCCTCCAGG ACCCTGAGAG TTATGTCCGA GCGAGTGCAG TGACCGCCAT GGGGCAGCTG
TCCAGCCAGG GCCTGCACGC CCCCACCAGC CCTGAGCATG CAGAGGCCCG GCAGAGCCTG
TTCCTGGAGC TCCTGCACAT CCTCTCCGTA GACTCGGAGG GCTTCCCACG GCGGGCGGTC
ATGCAAGTCT TCACTGAGTG GCTGCGGGAC GGCCACGCCG ACGCGGCCCA GGACACGGAG
CAGTTCGTGG CCACTGTGCT GCAGGCGGCG AGCCGAGACC TGGACTGGGA GGTCCGCGCC
CAGGGCCTGG AGCTGGCCCT CGTGTTCCTG GGCCAGACTT TGGGGCCGCC GCGTACCCAC
TGCCCCTATG CCGTGGCCCT ACCCGAGGTG GCCCCAGCCC AGCCACTCAC CGAGGCACTG
AGGGCTCTCT GCCACGTGGG GCTCTTTGAC TTCGCCTTTT GTGCCTTGTT TGACTGCGAC
CGCCCTGTGG CGCAGAAGTC TTGTGACCTC CTTCTCTTCC TGAGGGACAA GATTGCTTCC
TACAGCAGCC TGCGGGAGGC CAGGGGCAGC CCCAACACTG CCTCCGCAGA GGCCACCCTG
CCGAGGTGGC GGGCGGGTGA GCAGGCCCAG CCCCCAGGGG ACCAGGAGCC TGAGGCTGTG
CTGGCCATGC TCAGGTCCCT AGACCTGGAG GGCCTGCGGA GCACGCTGGC CGAGAGCAGC
GACCACGTGG AAAAGAGTCC CCAGTCCCTC CTGCAGGACA TGCTGGCCAC GGGAGGCTTC
CTGCAGGGGG ACGAGGCCGA CTGCTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011513483.1
CDS247..2796
Misc Feature(1)1594..1674
Misc Feature(2)order(1648..1653,1660..1662,1669..1674,1759..1764,1771..1773,1786..1791,1798..1800,1882..1887,1894..1896,1903..1908,1933..1935,2017..2022,2029..2031,2038..2043)
Misc Feature(3)1717..1800
Misc Feature(4)1828..1938
Misc Feature(5)1963..2043
Misc Feature(6)2215..2298
Translation

Target ORF information:

RefSeq Version XM_011515181.2
Organism Homo sapiens(Human)
Definition Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011515181.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGGACCCAG AATGCGCCCA GCTGCTCCCG GCTCTCTGTG CTGTTCTGGT AGATCCCAGG 
CAGCCGGTGG CAGATGACAC CTGTTTGGAG AAGCTCCTGG ACTGGTTTAA AACGGTCACT
GAAGGAGAGT CCAGTGTCGT GCTGCTGCAG GAGCACCCCT GCCTGGTGGA GCTGCTGTCC
CATGTGCTGA AAGTCCAGGA CCTGAGTTCT GGGGTCCTCT CCTTCTCACT GCGCCTGGCA
GGAACCTTCG CAGCCCAGGA AAACTGCTTC CAGTATCTTC AGCAGGGGGA GTTACTACCA
GGGCTCTTTG GGGAGCCAGG ACCCCTCGGC CGAGCAACCT GGGCCGTCCC CACCGTGCGC
AGCGGCTGGA TCCAGGGCCT GCGCTCCCTG GCACAGCACC CCAGCGCCCT GCGCTTCCTG
GCCGACCATG GTGCGGTCGA CACCATCTTC TCCCTGCAGG GAGACTCCAG CCTGTTTGTG
GCCTCGGCGG CCAGTCAGCT CCTGGTGCAC GTCCTGGCTT TGTCCATGCG AGGTGGAGCC
GAGGGGCAGC CCTGCCTGCC GGGGGGTGAC TGGCCCGCGT GTGCCCAGAA GATCATGGAT
CACGTTGAAG AGTCCTTGTG CTCCGCGGCC ACCCCCAAGG TCACTCAGGC CCTGAACGTC
CTGACCACGA CCTTCGGGCG CTGCCAGAGC CCCTGGACGG AAGCCCTGTG GGTGCGGCTG
AGTCCCCGCG TGGCCTGTCT GCTGGAGAGA GACCCCATCC CCGCCGCACA CTCGTTCGTG
GACCTGCTTC TCTGTGTGGC TCGTTCTCCC GTGTTCAGTT CTTCCGACGG CAGCCTGTGG
GAGACAGTGG CGCGGGCTCT GAGCTGCCTG GGTCCCACCC ACATGGGACC CCTGGCTTTG
GGGATCCTGA AGCTCGAGCA CTGTCCACAG GCACTGAGGA CCCAGGCCTT CCAGGTCCTT
CTCCAGCCCC TGGCCTGTGT CCTGAAGGCC ACGGTTCAGG CCCCCGGACC CCCAGGCTTG
CTGGACGGGA CGGCAGACGA TGCCACGACG GTGGACACAC TCCTGGCCTC CAAGTCGTCC
TGCGCCGGCC TCCTGTGCCG CACCCTGGCT CACCTGGAGG AGCTGCAGCC GCTGCCCCAG
CGCCCTTCAC CGTGGCCCCA GGCGTCTCTA CTGGGGGCTA CAGTGACTGT CCTGCGGCTC
TGTGACGGCT CGGCTGCCCC TGCCTCCAGT GTGGGGGGCC ACCTCTGTGG GACCCTGGCG
GGCTGCGTCC GGGTCCAGCG AGCAGCCCTC GACTTCCTGG GGACGCTGTC ACAGGGGACA
GGCCCCCAGG AGCTGGTGAC GCAGGCGCTT GCTGTCCTCC TGGAGTGCCT CGAGAGCCCC
GGCTCCAGCC CCACGGCCTC TGCGGATGGG GCCTTCACAC AGGGCCACAG AGCTGCTGCT
CAGAGAGCTT CACGATGCAG CCCACCCTAT CTGCTCCAGG TTCTGAAGAA GGCCTTCCAG
GCCACGCTCA GGTGGCTCCT GAGCTCACCC AAGACCCCCG GCTGCTCTGA TCTCGGCCCC
CTCATCCCGC AGTTCCTCAG AGAGCTGTTC CCTGTGCTGC AGAAACGCCT GTGCCACCCC
TGCTGGGAGG TGAGGGACTC CGCCCTCGAG TTCCTGACCC AGCTGAGCAG GCACTGGGGA
GGACAGGCTG ACTTCAGATG CGCACTCTTG GCTTCAGAGG TGCCTCAGCT GGCCCTGCAG
CTCCTCCAGG ACCCTGAGAG TTATGTCCGA GCGAGTGCAG TGACCGCCAT GGGGCAGCTG
TCCAGCCAGG GCCTGCACGC CCCCACCAGC CCTGAGCATG CAGAGGCCCG GCAGAGCCTG
TTCCTGGAGC TCCTGCACAT CCTCTCCGTA GACTCGGAGG GCTTCCCACG GCGGGCGGTC
ATGCAAGTCT TCACTGAGTG GCTGCGGGAC GGCCACGCCG ACGCGGCCCA GGACACGGAG
CAGTTCGTGG CCACTGTGCT GCAGGCGGCG AGCCGAGACC TGGACTGGGA GGTCCGCGCC
CAGGGCCTGG AGCTGGCCCT CGTGTTCCTG GGCCAGACTT TGGGGCCGCC GCGTACCCAC
TGCCCCTATG CCGTGGCCCT ACCCGAGGTG GCCCCAGCCC AGCCACTCAC CGAGGCACTG
AGGGCTCTCT GCCACGTGGG GCTCTTTGAC TTCGCCTTTT GTGCCTTGTT TGACTGCGAC
CGCCCTGTGG CGCAGAAGTC TTGTGACCTC CTTCTCTTCC TGAGGGACAA GATTGCTTCC
TACAGCAGCC TGCGGGAGGC CAGGGGCAGC CCCAACACTG CCTCCGCAGA GGCCACCCTG
CCGAGGTGGC GGGCGGGTGA GCAGGCCCAG CCCCCAGGGG ACCAGGAGCC TGAGGCTGTG
CTGGCCATGC TCAGGTCCCT AGACCTGGAG GGCCTGCGGA GCACGCTGGC CGAGAGCAGC
GACCACGTGG AAAAGAGTCC CCAGTCCCTC CTGCAGGACA TGCTGGCCAC GGGAGGCTTC
CTGCAGGGGG ACGAGGCCGA CTGCTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu78067
Clone ID Related Accession (Same CDS sequence) XM_011515184.2 , XM_011515183.1
Accession Version XM_011515184.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2205bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product BRCA1-associated ATM activator 1 isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515184.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGCGAGGTG GAGCCGAGGG GCAGCCCTGC CTGCCGGGGG GTGACTGGCC CGCGTGTGCC 
CAGAAGATCA TGGATCACGT TGAAGAGTCC TTGTGCTCCG CGGCCACCCC CAAGGTCACT
CAGGCCCTGA ACGTCCTGAC CACGACCTTC GGGCGCTGCC AGAGCCCCTG GACGGAAGCC
CTGTGGGTGC GGCTGAGTCC CCGCGTGGCC TGTCTGCTGG AGAGAGACCC CATCCCCGCC
GCACACTCGT TCGTGGACCT GCTTCTCTGT GTGGCTCGTT CTCCCGTGTT CAGTTCTTCC
GACGGCAGCC TGTGGGAGAC AGTGGCGCGG GCTCTGAGCT GCCTGGGTCC CACCCACATG
GGACCCCTGG CTTTGGGGAT CCTGAAGCTC GAGCACTGTC CACAGGCACT GAGGACCCAG
GCCTTCCAGG TCCTTCTCCA GCCCCTGGCC TGTGTCCTGA AGGCCACGGT TCAGGCCCCC
GGACCCCCAG GCTTGCTGGA CGGGACGGCA GACGATGCCA CGACGGTGGA CACACTCCTG
GCCTCCAAGT CGTCCTGCGC CGGCCTCCTG TGCCGCACCC TGGCTCACCT GGAGGAGCTG
CAGCCGCTGC CCCAGCGCCC TTCACCGTGG CCCCAGGCGT CTCTACTGGG GGCTACAGTG
ACTGTCCTGC GGCTCTGTGA CGGCTCGGCT GCCCCTGCCT CCAGTGTGGG GGGCCACCTC
TGTGGGACCC TGGCGGGCTG CGTCCGGGTC CAGCGAGCAG CCCTCGACTT CCTGGGGACG
CTGTCACAGG GGACAGGCCC CCAGGAGCTG GTGACGCAGG CGCTTGCTGT CCTCCTGGAG
TGCCTCGAGA GCCCCGGCTC CAGCCCCACG GCCTCTGCGG ATGGGGCCTT CACACAGGGC
CACAGAGCTG CTGCTCAGAG AGCTTCACGA TGCAGCCCAC CCTATCTGCT CCAGGTTCTG
AAGAAGGCCT TCCAGGCCAC GCTCAGGTGG CTCCTGAGCT CACCCAAGAC CCCCGGCTGC
TCTGATCTCG GCCCCCTCAT CCCGCAGTTC CTCAGAGAGC TGTTCCCTGT GCTGCAGAAA
CGCCTGTGCC ACCCCTGCTG GGAGGTGAGG GACTCCGCCC TCGAGTTCCT GACCCAGCTG
AGCAGGCACT GGGGAGGACA GGCTGACTTC AGATGCGCAC TCTTGGCTTC AGAGGTGCCT
CAGCTGGCCC TGCAGCTCCT CCAGGACCCT GAGAGTTATG TCCGAGCGAG TGCAGTGACC
GCCATGGGGC AGCTGTCCAG CCAGGGCCTG CACGCCCCCA CCAGCCCTGA GCATGCAGAG
GCCCGGCAGA TGGGTAAACT GAGGATTGGA GGGCCGTGTG CTCACTGCGC AGCATGGGAA
GGTGTCAGAG CGGGCTGTGG CCCTAGACTG CATGTCCGGG GCCAGCCCCC ATCATGTACC
GGAGTCCTGT TGCGGGAGCC TCGCTCCTGC CACCCCACCA ACCATCCGCA CTTGCTCCCT
GTCCCCCAGA GCCTGTTCCT GGAGCTCCTG CACATCCTCT CCGTAGACTC GGAGGGCTTC
CCACGGCGGG CGGTCATGCA AGTCTTCACT GAGTGGCTGC GGGACGGCCA CGCCGACGCG
GCCCAGGACA CGGAGCAGTT CGTGGCCACT GTGCTGCAGG CGGCGAGCCG AGACCTGGAC
TGGGAGGTCC GCGCCCAGGG CCTGGAGCTG GCCCTCGTGT TCCTGGGCCA GACTTTGGGG
CCGCCGCGTA CCCACTGCCC CTATGCCGTG GCCCTACCCG AGGTGGCCCC AGCCCAGCCA
CTCACCGAGG CACTGAGGGC TCTCTGCCAC GTGGGGCTCT TTGACTTCGC CTTTTGTGCC
TTGTTTGACT GCGACCGCCC TGTGGCGCAG AAGTCTTGTG ACCTCCTTCT CTTCCTGAGG
GACAAGATTG CTTCCTACAG CAGCCTGCGG GAGGCCAGGG GCAGCCCCAA CACTGCCTCC
GCAGAGGCCA CCCTGCCGAG GTGGCGGGCG GGTGAGCAGG CCCAGCCCCC AGGGGACCAG
GAGCCTGAGG CTGTGCTGGC CATGCTCAGG TCCCTAGACC TGGAGGGCCT GCGGAGCACG
CTGGCCGAGA GCAGCGACCA CGTGGAAAAG AGTCCCCAGT CCCTCCTGCA GGACATGCTG
GCCACGGGAG GCTTCCTGCA GGGGGACGAG GCCGACTGCT ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011513486.1
CDS567..2771
Misc Feature(1)1389..1469
Misc Feature(2)order(1443..1448,1455..1457,1464..1469,1554..1559,1566..1568,1581..1586,1593..1595,1677..1682,1689..1691,1698..1703,1728..1730,1812..1817,1824..1826,1833..1838)
Misc Feature(3)1512..1595
Misc Feature(4)1623..1733
Misc Feature(5)1758..1838
Translation

Target ORF information:

RefSeq Version XM_011515184.2
Organism Homo sapiens(Human)
Definition Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011515184.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGCGAGGTG GAGCCGAGGG GCAGCCCTGC CTGCCGGGGG GTGACTGGCC CGCGTGTGCC 
CAGAAGATCA TGGATCACGT TGAAGAGTCC TTGTGCTCCG CGGCCACCCC CAAGGTCACT
CAGGCCCTGA ACGTCCTGAC CACGACCTTC GGGCGCTGCC AGAGCCCCTG GACGGAAGCC
CTGTGGGTGC GGCTGAGTCC CCGCGTGGCC TGTCTGCTGG AGAGAGACCC CATCCCCGCC
GCACACTCGT TCGTGGACCT GCTTCTCTGT GTGGCTCGTT CTCCCGTGTT CAGTTCTTCC
GACGGCAGCC TGTGGGAGAC AGTGGCGCGG GCTCTGAGCT GCCTGGGTCC CACCCACATG
GGACCCCTGG CTTTGGGGAT CCTGAAGCTC GAGCACTGTC CACAGGCACT GAGGACCCAG
GCCTTCCAGG TCCTTCTCCA GCCCCTGGCC TGTGTCCTGA AGGCCACGGT TCAGGCCCCC
GGACCCCCAG GCTTGCTGGA CGGGACGGCA GACGATGCCA CGACGGTGGA CACACTCCTG
GCCTCCAAGT CGTCCTGCGC CGGCCTCCTG TGCCGCACCC TGGCTCACCT GGAGGAGCTG
CAGCCGCTGC CCCAGCGCCC TTCACCGTGG CCCCAGGCGT CTCTACTGGG GGCTACAGTG
ACTGTCCTGC GGCTCTGTGA CGGCTCGGCT GCCCCTGCCT CCAGTGTGGG GGGCCACCTC
TGTGGGACCC TGGCGGGCTG CGTCCGGGTC CAGCGAGCAG CCCTCGACTT CCTGGGGACG
CTGTCACAGG GGACAGGCCC CCAGGAGCTG GTGACGCAGG CGCTTGCTGT CCTCCTGGAG
TGCCTCGAGA GCCCCGGCTC CAGCCCCACG GCCTCTGCGG ATGGGGCCTT CACACAGGGC
CACAGAGCTG CTGCTCAGAG AGCTTCACGA TGCAGCCCAC CCTATCTGCT CCAGGTTCTG
AAGAAGGCCT TCCAGGCCAC GCTCAGGTGG CTCCTGAGCT CACCCAAGAC CCCCGGCTGC
TCTGATCTCG GCCCCCTCAT CCCGCAGTTC CTCAGAGAGC TGTTCCCTGT GCTGCAGAAA
CGCCTGTGCC ACCCCTGCTG GGAGGTGAGG GACTCCGCCC TCGAGTTCCT GACCCAGCTG
AGCAGGCACT GGGGAGGACA GGCTGACTTC AGATGCGCAC TCTTGGCTTC AGAGGTGCCT
CAGCTGGCCC TGCAGCTCCT CCAGGACCCT GAGAGTTATG TCCGAGCGAG TGCAGTGACC
GCCATGGGGC AGCTGTCCAG CCAGGGCCTG CACGCCCCCA CCAGCCCTGA GCATGCAGAG
GCCCGGCAGA TGGGTAAACT GAGGATTGGA GGGCCGTGTG CTCACTGCGC AGCATGGGAA
GGTGTCAGAG CGGGCTGTGG CCCTAGACTG CATGTCCGGG GCCAGCCCCC ATCATGTACC
GGAGTCCTGT TGCGGGAGCC TCGCTCCTGC CACCCCACCA ACCATCCGCA CTTGCTCCCT
GTCCCCCAGA GCCTGTTCCT GGAGCTCCTG CACATCCTCT CCGTAGACTC GGAGGGCTTC
CCACGGCGGG CGGTCATGCA AGTCTTCACT GAGTGGCTGC GGGACGGCCA CGCCGACGCG
GCCCAGGACA CGGAGCAGTT CGTGGCCACT GTGCTGCAGG CGGCGAGCCG AGACCTGGAC
TGGGAGGTCC GCGCCCAGGG CCTGGAGCTG GCCCTCGTGT TCCTGGGCCA GACTTTGGGG
CCGCCGCGTA CCCACTGCCC CTATGCCGTG GCCCTACCCG AGGTGGCCCC AGCCCAGCCA
CTCACCGAGG CACTGAGGGC TCTCTGCCAC GTGGGGCTCT TTGACTTCGC CTTTTGTGCC
TTGTTTGACT GCGACCGCCC TGTGGCGCAG AAGTCTTGTG ACCTCCTTCT CTTCCTGAGG
GACAAGATTG CTTCCTACAG CAGCCTGCGG GAGGCCAGGG GCAGCCCCAA CACTGCCTCC
GCAGAGGCCA CCCTGCCGAG GTGGCGGGCG GGTGAGCAGG CCCAGCCCCC AGGGGACCAG
GAGCCTGAGG CTGTGCTGGC CATGCTCAGG TCCCTAGACC TGGAGGGCCT GCGGAGCACG
CTGGCCGAGA GCAGCGACCA CGTGGAAAAG AGTCCCCAGT CCCTCCTGCA GGACATGCTG
GCCACGGGAG GCTTCCTGCA GGGGGACGAG GCCGACTGCT ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu78067
Clone ID Related Accession (Same CDS sequence) XM_011515184.2 , XM_011515183.1
Accession Version XM_011515183.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2205bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product BRCA1-associated ATM activator 1 isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGCGAGGTG GAGCCGAGGG GCAGCCCTGC CTGCCGGGGG GTGACTGGCC CGCGTGTGCC 
CAGAAGATCA TGGATCACGT TGAAGAGTCC TTGTGCTCCG CGGCCACCCC CAAGGTCACT
CAGGCCCTGA ACGTCCTGAC CACGACCTTC GGGCGCTGCC AGAGCCCCTG GACGGAAGCC
CTGTGGGTGC GGCTGAGTCC CCGCGTGGCC TGTCTGCTGG AGAGAGACCC CATCCCCGCC
GCACACTCGT TCGTGGACCT GCTTCTCTGT GTGGCTCGTT CTCCCGTGTT CAGTTCTTCC
GACGGCAGCC TGTGGGAGAC AGTGGCGCGG GCTCTGAGCT GCCTGGGTCC CACCCACATG
GGACCCCTGG CTTTGGGGAT CCTGAAGCTC GAGCACTGTC CACAGGCACT GAGGACCCAG
GCCTTCCAGG TCCTTCTCCA GCCCCTGGCC TGTGTCCTGA AGGCCACGGT TCAGGCCCCC
GGACCCCCAG GCTTGCTGGA CGGGACGGCA GACGATGCCA CGACGGTGGA CACACTCCTG
GCCTCCAAGT CGTCCTGCGC CGGCCTCCTG TGCCGCACCC TGGCTCACCT GGAGGAGCTG
CAGCCGCTGC CCCAGCGCCC TTCACCGTGG CCCCAGGCGT CTCTACTGGG GGCTACAGTG
ACTGTCCTGC GGCTCTGTGA CGGCTCGGCT GCCCCTGCCT CCAGTGTGGG GGGCCACCTC
TGTGGGACCC TGGCGGGCTG CGTCCGGGTC CAGCGAGCAG CCCTCGACTT CCTGGGGACG
CTGTCACAGG GGACAGGCCC CCAGGAGCTG GTGACGCAGG CGCTTGCTGT CCTCCTGGAG
TGCCTCGAGA GCCCCGGCTC CAGCCCCACG GCCTCTGCGG ATGGGGCCTT CACACAGGGC
CACAGAGCTG CTGCTCAGAG AGCTTCACGA TGCAGCCCAC CCTATCTGCT CCAGGTTCTG
AAGAAGGCCT TCCAGGCCAC GCTCAGGTGG CTCCTGAGCT CACCCAAGAC CCCCGGCTGC
TCTGATCTCG GCCCCCTCAT CCCGCAGTTC CTCAGAGAGC TGTTCCCTGT GCTGCAGAAA
CGCCTGTGCC ACCCCTGCTG GGAGGTGAGG GACTCCGCCC TCGAGTTCCT GACCCAGCTG
AGCAGGCACT GGGGAGGACA GGCTGACTTC AGATGCGCAC TCTTGGCTTC AGAGGTGCCT
CAGCTGGCCC TGCAGCTCCT CCAGGACCCT GAGAGTTATG TCCGAGCGAG TGCAGTGACC
GCCATGGGGC AGCTGTCCAG CCAGGGCCTG CACGCCCCCA CCAGCCCTGA GCATGCAGAG
GCCCGGCAGA TGGGTAAACT GAGGATTGGA GGGCCGTGTG CTCACTGCGC AGCATGGGAA
GGTGTCAGAG CGGGCTGTGG CCCTAGACTG CATGTCCGGG GCCAGCCCCC ATCATGTACC
GGAGTCCTGT TGCGGGAGCC TCGCTCCTGC CACCCCACCA ACCATCCGCA CTTGCTCCCT
GTCCCCCAGA GCCTGTTCCT GGAGCTCCTG CACATCCTCT CCGTAGACTC GGAGGGCTTC
CCACGGCGGG CGGTCATGCA AGTCTTCACT GAGTGGCTGC GGGACGGCCA CGCCGACGCG
GCCCAGGACA CGGAGCAGTT CGTGGCCACT GTGCTGCAGG CGGCGAGCCG AGACCTGGAC
TGGGAGGTCC GCGCCCAGGG CCTGGAGCTG GCCCTCGTGT TCCTGGGCCA GACTTTGGGG
CCGCCGCGTA CCCACTGCCC CTATGCCGTG GCCCTACCCG AGGTGGCCCC AGCCCAGCCA
CTCACCGAGG CACTGAGGGC TCTCTGCCAC GTGGGGCTCT TTGACTTCGC CTTTTGTGCC
TTGTTTGACT GCGACCGCCC TGTGGCGCAG AAGTCTTGTG ACCTCCTTCT CTTCCTGAGG
GACAAGATTG CTTCCTACAG CAGCCTGCGG GAGGCCAGGG GCAGCCCCAA CACTGCCTCC
GCAGAGGCCA CCCTGCCGAG GTGGCGGGCG GGTGAGCAGG CCCAGCCCCC AGGGGACCAG
GAGCCTGAGG CTGTGCTGGC CATGCTCAGG TCCCTAGACC TGGAGGGCCT GCGGAGCACG
CTGGCCGAGA GCAGCGACCA CGTGGAAAAG AGTCCCCAGT CCCTCCTGCA GGACATGCTG
GCCACGGGAG GCTTCCTGCA GGGGGACGAG GCCGACTGCT ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011513485.1
CDS661..2865
Misc Feature(1)1483..1563
Misc Feature(2)order(1537..1542,1549..1551,1558..1563,1648..1653,1660..1662,1675..1680,1687..1689,1771..1776,1783..1785,1792..1797,1822..1824,1906..1911,1918..1920,1927..1932)
Misc Feature(3)1606..1689
Misc Feature(4)1717..1827
Misc Feature(5)1852..1932
Translation

Target ORF information:

RefSeq Version XM_011515183.1
Organism Homo sapiens(Human)
Definition Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011515183.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGCGAGGTG GAGCCGAGGG GCAGCCCTGC CTGCCGGGGG GTGACTGGCC CGCGTGTGCC 
CAGAAGATCA TGGATCACGT TGAAGAGTCC TTGTGCTCCG CGGCCACCCC CAAGGTCACT
CAGGCCCTGA ACGTCCTGAC CACGACCTTC GGGCGCTGCC AGAGCCCCTG GACGGAAGCC
CTGTGGGTGC GGCTGAGTCC CCGCGTGGCC TGTCTGCTGG AGAGAGACCC CATCCCCGCC
GCACACTCGT TCGTGGACCT GCTTCTCTGT GTGGCTCGTT CTCCCGTGTT CAGTTCTTCC
GACGGCAGCC TGTGGGAGAC AGTGGCGCGG GCTCTGAGCT GCCTGGGTCC CACCCACATG
GGACCCCTGG CTTTGGGGAT CCTGAAGCTC GAGCACTGTC CACAGGCACT GAGGACCCAG
GCCTTCCAGG TCCTTCTCCA GCCCCTGGCC TGTGTCCTGA AGGCCACGGT TCAGGCCCCC
GGACCCCCAG GCTTGCTGGA CGGGACGGCA GACGATGCCA CGACGGTGGA CACACTCCTG
GCCTCCAAGT CGTCCTGCGC CGGCCTCCTG TGCCGCACCC TGGCTCACCT GGAGGAGCTG
CAGCCGCTGC CCCAGCGCCC TTCACCGTGG CCCCAGGCGT CTCTACTGGG GGCTACAGTG
ACTGTCCTGC GGCTCTGTGA CGGCTCGGCT GCCCCTGCCT CCAGTGTGGG GGGCCACCTC
TGTGGGACCC TGGCGGGCTG CGTCCGGGTC CAGCGAGCAG CCCTCGACTT CCTGGGGACG
CTGTCACAGG GGACAGGCCC CCAGGAGCTG GTGACGCAGG CGCTTGCTGT CCTCCTGGAG
TGCCTCGAGA GCCCCGGCTC CAGCCCCACG GCCTCTGCGG ATGGGGCCTT CACACAGGGC
CACAGAGCTG CTGCTCAGAG AGCTTCACGA TGCAGCCCAC CCTATCTGCT CCAGGTTCTG
AAGAAGGCCT TCCAGGCCAC GCTCAGGTGG CTCCTGAGCT CACCCAAGAC CCCCGGCTGC
TCTGATCTCG GCCCCCTCAT CCCGCAGTTC CTCAGAGAGC TGTTCCCTGT GCTGCAGAAA
CGCCTGTGCC ACCCCTGCTG GGAGGTGAGG GACTCCGCCC TCGAGTTCCT GACCCAGCTG
AGCAGGCACT GGGGAGGACA GGCTGACTTC AGATGCGCAC TCTTGGCTTC AGAGGTGCCT
CAGCTGGCCC TGCAGCTCCT CCAGGACCCT GAGAGTTATG TCCGAGCGAG TGCAGTGACC
GCCATGGGGC AGCTGTCCAG CCAGGGCCTG CACGCCCCCA CCAGCCCTGA GCATGCAGAG
GCCCGGCAGA TGGGTAAACT GAGGATTGGA GGGCCGTGTG CTCACTGCGC AGCATGGGAA
GGTGTCAGAG CGGGCTGTGG CCCTAGACTG CATGTCCGGG GCCAGCCCCC ATCATGTACC
GGAGTCCTGT TGCGGGAGCC TCGCTCCTGC CACCCCACCA ACCATCCGCA CTTGCTCCCT
GTCCCCCAGA GCCTGTTCCT GGAGCTCCTG CACATCCTCT CCGTAGACTC GGAGGGCTTC
CCACGGCGGG CGGTCATGCA AGTCTTCACT GAGTGGCTGC GGGACGGCCA CGCCGACGCG
GCCCAGGACA CGGAGCAGTT CGTGGCCACT GTGCTGCAGG CGGCGAGCCG AGACCTGGAC
TGGGAGGTCC GCGCCCAGGG CCTGGAGCTG GCCCTCGTGT TCCTGGGCCA GACTTTGGGG
CCGCCGCGTA CCCACTGCCC CTATGCCGTG GCCCTACCCG AGGTGGCCCC AGCCCAGCCA
CTCACCGAGG CACTGAGGGC TCTCTGCCAC GTGGGGCTCT TTGACTTCGC CTTTTGTGCC
TTGTTTGACT GCGACCGCCC TGTGGCGCAG AAGTCTTGTG ACCTCCTTCT CTTCCTGAGG
GACAAGATTG CTTCCTACAG CAGCCTGCGG GAGGCCAGGG GCAGCCCCAA CACTGCCTCC
GCAGAGGCCA CCCTGCCGAG GTGGCGGGCG GGTGAGCAGG CCCAGCCCCC AGGGGACCAG
GAGCCTGAGG CTGTGCTGGC CATGCTCAGG TCCCTAGACC TGGAGGGCCT GCGGAGCACG
CTGGCCGAGA GCAGCGACCA CGTGGAAAAG AGTCCCCAGT CCCTCCTGCA GGACATGCTG
GCCACGGGAG GCTTCCTGCA GGGGGACGAG GCCGACTGCT ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu97084
Clone ID Related Accession (Same CDS sequence) XM_017011833.1
Accession Version XM_017011833.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2643bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product BRCA1-associated ATM activator 1 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGGACCCAG AATGCGCCCA GCTGCTCCCG GCTCTCTGTG CTGTTCTGGT AGATCCCAGG 
CAGCCGGTGG CAGATGACAC CTGTTTGGAG AAGCTCCTGG ACTGGTTTAA AACGGTCACT
GAAGGAGAGT CCAGTGTCGT GCTGCTGCAG GAGCACCCCT GCCTGGTGGA GCTGCTGTCC
CATGTGCTGA AAGTCCAGGA CCTGAGTTCT GGGGTCCTCT CCTTCTCACT GCGCCTGGCA
GGAACCTTCG CAGCCCAGGA AAACTGCTTC CAGTATCTTC AGGGGGAGTT ACTACCAGGG
CTCTTTGGGG AGCCAGGACC CCTCGGCCGA GCAACCTGGG CCGTCCCCAC CGTGCGCAGC
GGCTGGATCC AGGGCCTGCG CTCCCTGGCA CAGCACCCCA GCGCCCTGCG CTTCCTGGCC
GACCATGGTG CGGTCGACAC CATCTTCTCC CTGCAGGGAG ACTCCAGCCT GTTTGTGGCC
TCGGCGGCCA GTCAGCTCCT GGTGCACGTC CTGGCTTTGT CCATGCGAGG TGGAGCCGAG
GGGCAGCCCT GCCTGCCGGG GGGTGACTGG CCCGCGTGTG CCCAGAAGAT CATGGATCAC
GTTGAAGAGT CCTTGTGCTC CGCGGCCACC CCCAAGGTCA CTCAGGCCCT GAACGTCCTG
ACCACGACCT TCGGGCGCTG CCAGAGCCCC TGGACGGAAG CCCTGTGGGT GCGGCTGAGT
CCCCGCGTGG CCTGTCTGCT GGAGAGAGAC CCCATCCCCG CCGCACACTC GTTCGTGGAC
CTGCTTCTCT GTGTGGCTCG TTCTCCCGTG TTCAGTTCTT CCGACGGCAG CCTGTGGGAG
ACAGTGGCGC GGGCTCTGAG CTGCCTGGGT CCCACCCACA TGGGACCCCT GGCTTTGGGG
ATCCTGAAGC TCGAGCACTG TCCACAGGCA CTGAGGACCC AGGCCTTCCA GGTCCTTCTC
CAGCCCCTGG CCTGTGTCCT GAAGGCCACG GTTCAGGCCC CCGGACCCCC AGGCTTGCTG
GACGGGACGG CAGACGATGC CACGACGGTG GACACACTCC TGGCCTCCAA GTCGTCCTGC
GCCGGCCTCC TGTGCCGCAC CCTGGCTCAC CTGGAGGAGC TGCAGCCGCT GCCCCAGCGC
CCTTCACCGT GGCCCCAGGC GTCTCTACTG GGGGCTACAG TGACTGTCCT GCGGCTCTGT
GACGGCTCGG CTGCCCCTGC CTCCAGTGTG GGGGGCCACC TCTGTGGGAC CCTGGCGGGC
TGCGTCCGGG TCCAGCGAGC AGCCCTCGAC TTCCTGGGGA CGCTGTCACA GGGGACAGGC
CCCCAGGAGC TGGTGACGCA GGCGCTTGCT GTCCTCCTGG AGTGCCTCGA GAGCCCCGGC
TCCAGCCCCA CGGTTCTGAA GAAGGCCTTC CAGGCCACGC TCAGGTGGCT CCTGAGCTCA
CCCAAGACCC CCGGCTGCTC TGATCTCGGC CCCCTCATCC CGCAGTTCCT CAGAGAGCTG
TTCCCTGTGC TGCAGAAACG CCTGTGCCAC CCCTGCTGGG AGGTGAGGGA CTCCGCCCTC
GAGTTCCTGA CCCAGCTGAG CAGGCACTGG GGAGGACAGG CTGACTTCAG ATGCGCACTC
TTGGCTTCAG AGGTGCCTCA GCTGGCCCTG CAGCTCCTCC AGGACCCTGA GAGTTATGTC
CGAGCGAGTG CAGTGACCGC CATGGGGCAG CTGTCCAGCC AGGGCCTGCA CGCCCCCACC
AGCCCTGAGC ATGCAGAGGC CCGGCAGATG GGTAAACTGA GGATTGGAGG GCCGTGTGCT
CACTGCGCAG CATGGGAAGG TGTCAGAGCG GGCTGTGGCC CTAGACTGCA TGTCCGGGGC
CAGCCCCCAT CATGTACCGG AGTCCTGTTG CGGGAGCCTC GCTCCTGCCA CCCCACCAAC
CATCCGCACT TGCTCCCTGT CCCCCAGAGC CTGTTCCTGG AGCTCCTGCA CATCCTCTCC
GTAGACTCGG AGGGCTTCCC ACGGCGGGCG GTCATGCAAG TCTTCACTGA GTGGCTGCGG
GACGGCCACG CCGACGCGGC CCAGGACACG GAGCAGTTCG TGGCCACTGT GCTGCAGGCG
GCGAGCCGAG ACCTGGACTG GGAGGTCCGC GCCCAGGGCC TGGAGCTGGC CCTCGTGTTC
CTGGGCCAGA CTTTGGGGCC GCCGCGTACC CACTGCCCCT ATGCCGTGGC CCTACCCGAG
GTGGCCCCAG CCCAGCCACT CACCGAGGCA CTGAGGGCTC TCTGCCACGT GGGGCTCTTT
GACTTCGCCT TTTGTGCCTT GTTTGACTGC GACCGCCCTG TGGCGCAGAA GTCTTGTGAC
CTCCTTCTCT TCCTGAGGGA CAAGATTGCT TCCTACAGCA GCCTGCGGGA GGCCAGGGGC
AGCCCCAACA CTGCCTCCGC AGAGGCCACC CTGCCGAGGT GGCGGGCGGG TGAGCAGGCC
CAGCCCCCAG GGGACCAGGA GCCTGAGGCT GTGCTGGCCA TGCTCAGGTC CCTAGACCTG
GAGGGCCTGC GGAGCACGCT GGCCGAGAGC AGCGACCACG TGGAAAAGAG TCCCCAGTCC
CTCCTGCAGG ACATGCTGGC CACGGGAGGC TTCCTGCAGG GGGACGAGGC CGACTGCTAC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016867322.1
CDS55..2697
Translation

Target ORF information:

RefSeq Version XM_017011833.1
Organism Homo sapiens(Human)
Definition Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017011833.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGGACCCAG AATGCGCCCA GCTGCTCCCG GCTCTCTGTG CTGTTCTGGT AGATCCCAGG 
CAGCCGGTGG CAGATGACAC CTGTTTGGAG AAGCTCCTGG ACTGGTTTAA AACGGTCACT
GAAGGAGAGT CCAGTGTCGT GCTGCTGCAG GAGCACCCCT GCCTGGTGGA GCTGCTGTCC
CATGTGCTGA AAGTCCAGGA CCTGAGTTCT GGGGTCCTCT CCTTCTCACT GCGCCTGGCA
GGAACCTTCG CAGCCCAGGA AAACTGCTTC CAGTATCTTC AGGGGGAGTT ACTACCAGGG
CTCTTTGGGG AGCCAGGACC CCTCGGCCGA GCAACCTGGG CCGTCCCCAC CGTGCGCAGC
GGCTGGATCC AGGGCCTGCG CTCCCTGGCA CAGCACCCCA GCGCCCTGCG CTTCCTGGCC
GACCATGGTG CGGTCGACAC CATCTTCTCC CTGCAGGGAG ACTCCAGCCT GTTTGTGGCC
TCGGCGGCCA GTCAGCTCCT GGTGCACGTC CTGGCTTTGT CCATGCGAGG TGGAGCCGAG
GGGCAGCCCT GCCTGCCGGG GGGTGACTGG CCCGCGTGTG CCCAGAAGAT CATGGATCAC
GTTGAAGAGT CCTTGTGCTC CGCGGCCACC CCCAAGGTCA CTCAGGCCCT GAACGTCCTG
ACCACGACCT TCGGGCGCTG CCAGAGCCCC TGGACGGAAG CCCTGTGGGT GCGGCTGAGT
CCCCGCGTGG CCTGTCTGCT GGAGAGAGAC CCCATCCCCG CCGCACACTC GTTCGTGGAC
CTGCTTCTCT GTGTGGCTCG TTCTCCCGTG TTCAGTTCTT CCGACGGCAG CCTGTGGGAG
ACAGTGGCGC GGGCTCTGAG CTGCCTGGGT CCCACCCACA TGGGACCCCT GGCTTTGGGG
ATCCTGAAGC TCGAGCACTG TCCACAGGCA CTGAGGACCC AGGCCTTCCA GGTCCTTCTC
CAGCCCCTGG CCTGTGTCCT GAAGGCCACG GTTCAGGCCC CCGGACCCCC AGGCTTGCTG
GACGGGACGG CAGACGATGC CACGACGGTG GACACACTCC TGGCCTCCAA GTCGTCCTGC
GCCGGCCTCC TGTGCCGCAC CCTGGCTCAC CTGGAGGAGC TGCAGCCGCT GCCCCAGCGC
CCTTCACCGT GGCCCCAGGC GTCTCTACTG GGGGCTACAG TGACTGTCCT GCGGCTCTGT
GACGGCTCGG CTGCCCCTGC CTCCAGTGTG GGGGGCCACC TCTGTGGGAC CCTGGCGGGC
TGCGTCCGGG TCCAGCGAGC AGCCCTCGAC TTCCTGGGGA CGCTGTCACA GGGGACAGGC
CCCCAGGAGC TGGTGACGCA GGCGCTTGCT GTCCTCCTGG AGTGCCTCGA GAGCCCCGGC
TCCAGCCCCA CGGTTCTGAA GAAGGCCTTC CAGGCCACGC TCAGGTGGCT CCTGAGCTCA
CCCAAGACCC CCGGCTGCTC TGATCTCGGC CCCCTCATCC CGCAGTTCCT CAGAGAGCTG
TTCCCTGTGC TGCAGAAACG CCTGTGCCAC CCCTGCTGGG AGGTGAGGGA CTCCGCCCTC
GAGTTCCTGA CCCAGCTGAG CAGGCACTGG GGAGGACAGG CTGACTTCAG ATGCGCACTC
TTGGCTTCAG AGGTGCCTCA GCTGGCCCTG CAGCTCCTCC AGGACCCTGA GAGTTATGTC
CGAGCGAGTG CAGTGACCGC CATGGGGCAG CTGTCCAGCC AGGGCCTGCA CGCCCCCACC
AGCCCTGAGC ATGCAGAGGC CCGGCAGATG GGTAAACTGA GGATTGGAGG GCCGTGTGCT
CACTGCGCAG CATGGGAAGG TGTCAGAGCG GGCTGTGGCC CTAGACTGCA TGTCCGGGGC
CAGCCCCCAT CATGTACCGG AGTCCTGTTG CGGGAGCCTC GCTCCTGCCA CCCCACCAAC
CATCCGCACT TGCTCCCTGT CCCCCAGAGC CTGTTCCTGG AGCTCCTGCA CATCCTCTCC
GTAGACTCGG AGGGCTTCCC ACGGCGGGCG GTCATGCAAG TCTTCACTGA GTGGCTGCGG
GACGGCCACG CCGACGCGGC CCAGGACACG GAGCAGTTCG TGGCCACTGT GCTGCAGGCG
GCGAGCCGAG ACCTGGACTG GGAGGTCCGC GCCCAGGGCC TGGAGCTGGC CCTCGTGTTC
CTGGGCCAGA CTTTGGGGCC GCCGCGTACC CACTGCCCCT ATGCCGTGGC CCTACCCGAG
GTGGCCCCAG CCCAGCCACT CACCGAGGCA CTGAGGGCTC TCTGCCACGT GGGGCTCTTT
GACTTCGCCT TTTGTGCCTT GTTTGACTGC GACCGCCCTG TGGCGCAGAA GTCTTGTGAC
CTCCTTCTCT TCCTGAGGGA CAAGATTGCT TCCTACAGCA GCCTGCGGGA GGCCAGGGGC
AGCCCCAACA CTGCCTCCGC AGAGGCCACC CTGCCGAGGT GGCGGGCGGG TGAGCAGGCC
CAGCCCCCAG GGGACCAGGA GCCTGAGGCT GTGCTGGCCA TGCTCAGGTC CCTAGACCTG
GAGGGCCTGC GGAGCACGCT GGCCGAGAGC AGCGACCACG TGGAAAAGAG TCCCCAGTCC
CTCCTGCAGG ACATGCTGGC CACGGGAGGC TTCCTGCAGG GGGACGAGGC CGACTGCTAC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu97085
Clone ID Related Accession (Same CDS sequence) XM_017011834.1
Accession Version XM_017011834.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2463bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product BRCA1-associated ATM activator 1 isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGACCCAG AATGCGCCCA GCTGCTCCCG GCTCTCTGTG CTGTTCTGGT AGATCCCAGG 
CAGCCGGTGG CAGATGACAC CTGTTTGGAG AAGCTCCTGG ACTGGTTTAA AACGGTCACT
GAAGGAGAGT CCAGTGTCGT GCTGCTGCAG GAGCACCCCT GCCTGGTGGA GCTGCTGTCC
CATGTGCTGA AAGTCCAGGA CCTGAGTTCT GGGGTCCTCT CCTTCTCACT GCGCCTGGCA
GGAACCTTCG CAGCCCAGGA AAACTGCTTC CAGTATCTTC AGGGGGAGTT ACTACCAGGG
CTCTTTGGGG AGCCAGGACC CCTCGGCCGA GCAACCTGGG CCGTCCCCAC CGTGCGCAGC
GGCTGGATCC AGGGCCTGCG CTCCCTGGCA CAGCACCCCA GCGCCCTGCG CTTCCTGGCC
GACCATGGTG CGGTCGACAC CATCTTCTCC CTGCAGGGAG ACTCCAGCCT GTTTGTGGCC
TCGGCGGCCA GTCAGCTCCT GGTGCACGTC CTGGCTTTGT CCATGCGAGG TGGAGCCGAG
GGGCAGCCCT GCCTGCCGGG GGGTGACTGG CCCGCGTGTG CCCAGAAGAT CATGGATCAC
GTTGAAGAGT CCTTGTGCTC CGCGGCCACC CCCAAGGTCA CTCAGGCCCT GAACGTCCTG
ACCACGACCT TCGGGCGCTG CCAGAGCCCC TGGACGGAAG CCCTGTGGGT GCGGCTGAGT
CCCCGCGTGG CCTGTCTGCT GGAGAGAGAC CCCATCCCCG CCGCACACTC GTTCGTGGAC
CTGCTTCTCT GTGTGGCTCG TTCTCCCGTG TTCAGTTCTT CCGACGGCAG CCTGTGGGAG
ACAGTGGCGC GGGCTCTGAG CTGCCTGGGT CCCACCCACA TGGGACCCCT GGCTTTGGGG
ATCCTGAAGC TCGAGCACTG TCCACAGGCA CTGAGGACCC AGGCCTTCCA GGTCCTTCTC
CAGCCCCTGG CCTGTGTCCT GAAGGCCACG GTTCAGGCCC CCGGACCCCC AGGCTTGCTG
GACGGGACGG CAGACGATGC CACGACGGTG GACACACTCC TGGCCTCCAA GTCGTCCTGC
GCCGGCCTCC TGTGCCGCAC CCTGGCTCAC CTGGAGGAGC TGCAGCCGCT GCCCCAGCGC
CCTTCACCGT GGCCCCAGGC GTCTCTACTG GGGGCTACAG TGACTGTCCT GCGGCTCTGT
GACGGCTCGG CTGCCCCTGC CTCCAGTGTG GGGGGCCACC TCTGTGGGAC CCTGGCGGGC
TGCGTCCGGG TCCAGCGAGC AGCCCTCGAC TTCCTGGGGA CGCTGTCACA GGGGACAGGC
CCCCAGGAGC TGGTGACGCA GGCGCTTGCT GTCCTCCTGG AGTGCCTCGA GAGCCCCGGC
TCCAGCCCCA CGGTTCTGAA GAAGGCCTTC CAGGCCACGC TCAGGTGGCT CCTGAGCTCA
CCCAAGACCC CCGGCTGCTC TGATCTCGGC CCCCTCATCC CGCAGTTCCT CAGAGAGCTG
TTCCCTGTGC TGCAGAAACG CCTGTGCCAC CCCTGCTGGG AGGTGAGGGA CTCCGCCCTC
GAGTTCCTGA CCCAGCTGAG CAGGCACTGG GGAGGACAGG CTGACTTCAG ATGCGCACTC
TTGGCTTCAG AGGTGCCTCA GCTGGCCCTG CAGCTCCTCC AGGACCCTGA GAGTTATGTC
CGAGCGAGTG CAGTGACCGC CATGGGGCAG CTGTCCAGCC AGGGCCTGCA CGCCCCCACC
AGCCCTGAGC ATGCAGAGGC CCGGCAGAGC CTGTTCCTGG AGCTCCTGCA CATCCTCTCC
GTAGACTCGG AGGGCTTCCC ACGGCGGGCG GTCATGCAAG TCTTCACTGA GTGGCTGCGG
GACGGCCACG CCGACGCGGC CCAGGACACG GAGCAGTTCG TGGCCACTGT GCTGCAGGCG
GCGAGCCGAG ACCTGGACTG GGAGGTCCGC GCCCAGGGCC TGGAGCTGGC CCTCGTGTTC
CTGGGCCAGA CTTTGGGGCC GCCGCGTACC CACTGCCCCT ATGCCGTGGC CCTACCCGAG
GTGGCCCCAG CCCAGCCACT CACCGAGGCA CTGAGGGCTC TCTGCCACGT GGGGCTCTTT
GACTTCGCCT TTTGTGCCTT GTTTGACTGC GACCGCCCTG TGGCGCAGAA GTCTTGTGAC
CTCCTTCTCT TCCTGAGGGA CAAGATTGCT TCCTACAGCA GCCTGCGGGA GGCCAGGGGC
AGCCCCAACA CTGCCTCCGC AGAGGCCACC CTGCCGAGGT GGCGGGCGGG TGAGCAGGCC
CAGCCCCCAG GGGACCAGGA GCCTGAGGCT GTGCTGGCCA TGCTCAGGTC CCTAGACCTG
GAGGGCCTGC GGAGCACGCT GGCCGAGAGC AGCGACCACG TGGAAAAGAG TCCCCAGTCC
CTCCTGCAGG ACATGCTGGC CACGGGAGGC TTCCTGCAGG GGGACGAGGC CGACTGCTAC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016867323.1
CDS246..2708
Translation

Target ORF information:

RefSeq Version XM_017011834.1
Organism Homo sapiens(Human)
Definition Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017011834.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGACCCAG AATGCGCCCA GCTGCTCCCG GCTCTCTGTG CTGTTCTGGT AGATCCCAGG 
CAGCCGGTGG CAGATGACAC CTGTTTGGAG AAGCTCCTGG ACTGGTTTAA AACGGTCACT
GAAGGAGAGT CCAGTGTCGT GCTGCTGCAG GAGCACCCCT GCCTGGTGGA GCTGCTGTCC
CATGTGCTGA AAGTCCAGGA CCTGAGTTCT GGGGTCCTCT CCTTCTCACT GCGCCTGGCA
GGAACCTTCG CAGCCCAGGA AAACTGCTTC CAGTATCTTC AGGGGGAGTT ACTACCAGGG
CTCTTTGGGG AGCCAGGACC CCTCGGCCGA GCAACCTGGG CCGTCCCCAC CGTGCGCAGC
GGCTGGATCC AGGGCCTGCG CTCCCTGGCA CAGCACCCCA GCGCCCTGCG CTTCCTGGCC
GACCATGGTG CGGTCGACAC CATCTTCTCC CTGCAGGGAG ACTCCAGCCT GTTTGTGGCC
TCGGCGGCCA GTCAGCTCCT GGTGCACGTC CTGGCTTTGT CCATGCGAGG TGGAGCCGAG
GGGCAGCCCT GCCTGCCGGG GGGTGACTGG CCCGCGTGTG CCCAGAAGAT CATGGATCAC
GTTGAAGAGT CCTTGTGCTC CGCGGCCACC CCCAAGGTCA CTCAGGCCCT GAACGTCCTG
ACCACGACCT TCGGGCGCTG CCAGAGCCCC TGGACGGAAG CCCTGTGGGT GCGGCTGAGT
CCCCGCGTGG CCTGTCTGCT GGAGAGAGAC CCCATCCCCG CCGCACACTC GTTCGTGGAC
CTGCTTCTCT GTGTGGCTCG TTCTCCCGTG TTCAGTTCTT CCGACGGCAG CCTGTGGGAG
ACAGTGGCGC GGGCTCTGAG CTGCCTGGGT CCCACCCACA TGGGACCCCT GGCTTTGGGG
ATCCTGAAGC TCGAGCACTG TCCACAGGCA CTGAGGACCC AGGCCTTCCA GGTCCTTCTC
CAGCCCCTGG CCTGTGTCCT GAAGGCCACG GTTCAGGCCC CCGGACCCCC AGGCTTGCTG
GACGGGACGG CAGACGATGC CACGACGGTG GACACACTCC TGGCCTCCAA GTCGTCCTGC
GCCGGCCTCC TGTGCCGCAC CCTGGCTCAC CTGGAGGAGC TGCAGCCGCT GCCCCAGCGC
CCTTCACCGT GGCCCCAGGC GTCTCTACTG GGGGCTACAG TGACTGTCCT GCGGCTCTGT
GACGGCTCGG CTGCCCCTGC CTCCAGTGTG GGGGGCCACC TCTGTGGGAC CCTGGCGGGC
TGCGTCCGGG TCCAGCGAGC AGCCCTCGAC TTCCTGGGGA CGCTGTCACA GGGGACAGGC
CCCCAGGAGC TGGTGACGCA GGCGCTTGCT GTCCTCCTGG AGTGCCTCGA GAGCCCCGGC
TCCAGCCCCA CGGTTCTGAA GAAGGCCTTC CAGGCCACGC TCAGGTGGCT CCTGAGCTCA
CCCAAGACCC CCGGCTGCTC TGATCTCGGC CCCCTCATCC CGCAGTTCCT CAGAGAGCTG
TTCCCTGTGC TGCAGAAACG CCTGTGCCAC CCCTGCTGGG AGGTGAGGGA CTCCGCCCTC
GAGTTCCTGA CCCAGCTGAG CAGGCACTGG GGAGGACAGG CTGACTTCAG ATGCGCACTC
TTGGCTTCAG AGGTGCCTCA GCTGGCCCTG CAGCTCCTCC AGGACCCTGA GAGTTATGTC
CGAGCGAGTG CAGTGACCGC CATGGGGCAG CTGTCCAGCC AGGGCCTGCA CGCCCCCACC
AGCCCTGAGC ATGCAGAGGC CCGGCAGAGC CTGTTCCTGG AGCTCCTGCA CATCCTCTCC
GTAGACTCGG AGGGCTTCCC ACGGCGGGCG GTCATGCAAG TCTTCACTGA GTGGCTGCGG
GACGGCCACG CCGACGCGGC CCAGGACACG GAGCAGTTCG TGGCCACTGT GCTGCAGGCG
GCGAGCCGAG ACCTGGACTG GGAGGTCCGC GCCCAGGGCC TGGAGCTGGC CCTCGTGTTC
CTGGGCCAGA CTTTGGGGCC GCCGCGTACC CACTGCCCCT ATGCCGTGGC CCTACCCGAG
GTGGCCCCAG CCCAGCCACT CACCGAGGCA CTGAGGGCTC TCTGCCACGT GGGGCTCTTT
GACTTCGCCT TTTGTGCCTT GTTTGACTGC GACCGCCCTG TGGCGCAGAA GTCTTGTGAC
CTCCTTCTCT TCCTGAGGGA CAAGATTGCT TCCTACAGCA GCCTGCGGGA GGCCAGGGGC
AGCCCCAACA CTGCCTCCGC AGAGGCCACC CTGCCGAGGT GGCGGGCGGG TGAGCAGGCC
CAGCCCCCAG GGGACCAGGA GCCTGAGGCT GTGCTGGCCA TGCTCAGGTC CCTAGACCTG
GAGGGCCTGC GGAGCACGCT GGCCGAGAGC AGCGACCACG TGGAAAAGAG TCCCCAGTCC
CTCCTGCAGG ACATGCTGGC CACGGGAGGC TTCCTGCAGG GGGACGAGGC CGACTGCTAC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu97086
Clone ID Related Accession (Same CDS sequence) XM_017011836.1
Accession Version XM_017011836.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1680bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product BRCA1-associated ATM activator 1 isoform X10
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGACCCAG AATGCGCCCA GCTGCTCCCG GCTCTCTGTG CTGTTCTGGT AGATCCCAGG 
CAGCCGGTGG CAGATGACAC CTGTTTGGAG AAGCTCCTGG ACTGGTTTAA AACGGTCACT
GAAGGAGAGT CCAGTGTCGT GCTGCTGCAG GAGCACCCCT GCCTGGTGGA GCTGCTGTCC
CATGTGCTGA AAGTCCAGGA CCTGAGTTCT GGGGTCCTCT CCTTCTCACT GCGCCTGGCA
GGAACCTTCG CAGCCCAGGA AAACTGCTTC CAGTATCTTC AGCAGGGGGA GTTACTACCA
GGGCTCTTTG GGGAGCCAGG ACCCCTCGGC CGAGCAACCT GGGCCGTCCC CACCGTGCGC
AGCGGCTGGA TCCAGGGCCT GCGCTCCCTG GCACAGCACC CCAGCGCCCT GCGCTTCCTG
GCCGACCATG GTGCGGTCGA CACCATCTTC TCCCTGCAGG GAGACTCCAG CCTGTTTGTG
GCCTCGGCGG CCAGTCAGCT CCTGGTGCAC GTCCTGGCTT TGTCCATGCG AGGTGGAGCC
GAGGGGCAGC CCTGCCTGCC GGGGGGTGAC TGGCCCGCGT GTGCCCAGAA GATCATGGAT
CACGTTGAAG AGTCCTTGTG CTCCGCGGCC ACCCCCAAGG TCACTCAGGC CCTGAACGTC
CTGACCACGA CCTTCGGGCG CTGCCAGAGC CCCTGGACGG AAGCCCTGTG GGTGCGGCTG
AGTCCCCGCG TGGCCTGTCT GCTGGAGAGA GACCCCATCC CCGCCGCACA CTCGTTCGTG
GACCTGCTTC TCTGTGTGGC TCGTTCTCCC GTGTTCAGTT CTTCCGACGG CAGCCTGTGG
GAGACAGTGG CGCGGGCTCT GAGCTGCCTG GGTCCCACCC ACATGGGACC CCTGGCTTTG
GGGATCCTGA AGCTCGAGCA CTGTCCACAG GCACTGAGGA CCCAGGCCTT CCAGGTCCTT
CTCCAGCCCC TGGCCTGTGT CCTGAAGGCC ACGGTTCAGG CCCCCGGACC CCCAGGCTTG
CTGGACGGGA CGGCAGACGA TGCCACGACG GTGGACACAC TCCTGGCCTC CAAGTCGTCC
TGCGCCGGCC TCCTGTGCCG CACCCTGGCT CACCTGGAGG AGCTGCAGCC GCTGCCCCAG
CGCCCTTCAC CGTGGCCCCA GGCGTCTCTA CTGGGGGCTA CAGTGACTGT CCTGCGGCTC
TGTGACGGCT CGGCTGCCCC TGCCTCCAGT GTGGGGGGCC ACCTCTGTGG GACCCTGGCG
GGCTGCGTCC GGGTCCAGCG AGCAGCCCTC GACTTCCTGG GGACGCTGTC ACAGGGGACA
GGCCCCCAGG AGCTGGTGAC GCAGGCGCTT GCTGTCCTCC TGGAGTGCCT CGAGAGCCCC
GGCTCCAGCC CCACGGTTCT GAAGAAGGCC TTCCAGGCCA CGCTCAGGTG GCTCCTGAGC
TCACCCAAGA CCCCCGGCTG CTCTGATCTC GGCCCCCTCA TCCCGCAGTT CCTCAGAGAG
CTGTTCCCTG TGCTGCAGAA ACGCCTGTGC CACCCCTGCT GGGAGGTGAG GGACTCCGCC
CTCGAGTTCC TGACCCAGCT GAGCAGGCAC TGGGGAGAGC CTGTTCCTGG AGCTCCTGCA
CATCCTCTCC GTAGACTCGG AGGGCTTCCC ACGGCGGGCG GTCATGCAAG TCTTCACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016867325.1
CDS245..1924
Translation

Target ORF information:

RefSeq Version XM_017011836.1
Organism Homo sapiens(Human)
Definition Homo sapiens BRCA1 associated ATM activator 1 (BRAT1), transcript variant X13, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017011836.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGACCCAG AATGCGCCCA GCTGCTCCCG GCTCTCTGTG CTGTTCTGGT AGATCCCAGG 
CAGCCGGTGG CAGATGACAC CTGTTTGGAG AAGCTCCTGG ACTGGTTTAA AACGGTCACT
GAAGGAGAGT CCAGTGTCGT GCTGCTGCAG GAGCACCCCT GCCTGGTGGA GCTGCTGTCC
CATGTGCTGA AAGTCCAGGA CCTGAGTTCT GGGGTCCTCT CCTTCTCACT GCGCCTGGCA
GGAACCTTCG CAGCCCAGGA AAACTGCTTC CAGTATCTTC AGCAGGGGGA GTTACTACCA
GGGCTCTTTG GGGAGCCAGG ACCCCTCGGC CGAGCAACCT GGGCCGTCCC CACCGTGCGC
AGCGGCTGGA TCCAGGGCCT GCGCTCCCTG GCACAGCACC CCAGCGCCCT GCGCTTCCTG
GCCGACCATG GTGCGGTCGA CACCATCTTC TCCCTGCAGG GAGACTCCAG CCTGTTTGTG
GCCTCGGCGG CCAGTCAGCT CCTGGTGCAC GTCCTGGCTT TGTCCATGCG AGGTGGAGCC
GAGGGGCAGC CCTGCCTGCC GGGGGGTGAC TGGCCCGCGT GTGCCCAGAA GATCATGGAT
CACGTTGAAG AGTCCTTGTG CTCCGCGGCC ACCCCCAAGG TCACTCAGGC CCTGAACGTC
CTGACCACGA CCTTCGGGCG CTGCCAGAGC CCCTGGACGG AAGCCCTGTG GGTGCGGCTG
AGTCCCCGCG TGGCCTGTCT GCTGGAGAGA GACCCCATCC CCGCCGCACA CTCGTTCGTG
GACCTGCTTC TCTGTGTGGC TCGTTCTCCC GTGTTCAGTT CTTCCGACGG CAGCCTGTGG
GAGACAGTGG CGCGGGCTCT GAGCTGCCTG GGTCCCACCC ACATGGGACC CCTGGCTTTG
GGGATCCTGA AGCTCGAGCA CTGTCCACAG GCACTGAGGA CCCAGGCCTT CCAGGTCCTT
CTCCAGCCCC TGGCCTGTGT CCTGAAGGCC ACGGTTCAGG CCCCCGGACC CCCAGGCTTG
CTGGACGGGA CGGCAGACGA TGCCACGACG GTGGACACAC TCCTGGCCTC CAAGTCGTCC
TGCGCCGGCC TCCTGTGCCG CACCCTGGCT CACCTGGAGG AGCTGCAGCC GCTGCCCCAG
CGCCCTTCAC CGTGGCCCCA GGCGTCTCTA CTGGGGGCTA CAGTGACTGT CCTGCGGCTC
TGTGACGGCT CGGCTGCCCC TGCCTCCAGT GTGGGGGGCC ACCTCTGTGG GACCCTGGCG
GGCTGCGTCC GGGTCCAGCG AGCAGCCCTC GACTTCCTGG GGACGCTGTC ACAGGGGACA
GGCCCCCAGG AGCTGGTGAC GCAGGCGCTT GCTGTCCTCC TGGAGTGCCT CGAGAGCCCC
GGCTCCAGCC CCACGGTTCT GAAGAAGGCC TTCCAGGCCA CGCTCAGGTG GCTCCTGAGC
TCACCCAAGA CCCCCGGCTG CTCTGATCTC GGCCCCCTCA TCCCGCAGTT CCTCAGAGAG
CTGTTCCCTG TGCTGCAGAA ACGCCTGTGC CACCCCTGCT GGGAGGTGAG GGACTCCGCC
CTCGAGTTCC TGACCCAGCT GAGCAGGCAC TGGGGAGAGC CTGTTCCTGG AGCTCCTGCA
CATCCTCTCC GTAGACTCGG AGGGCTTCCC ACGGCGGGCG GTCATGCAAG TCTTCACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Early-Onset Severe Encephalopathy with Epilepsy: The BRAT1 Gene Should Be Added to the List of Causes.
Neuropediatrics46(6)392-400(2015 Dec)
van de Pol LA,Wolf NI,van Weissenbruch MM,Stam CJ,Weiss JM,Waisfisz Q,Kevelam SH,Bugiani M,van de Kamp JM,van der Knaap MS


book

Nedd4 family interacting protein 1 (Ndfip1) is required for ubiquitination and nuclear trafficking of BRCA1-associated ATM activator 1 (BRAT1) during the DNA damage response.
The Journal of biological chemistry290(11)7141-50(2015 Mar)
Low LH,Chow YL,Li Y,Goh CP,Putz U,Silke J,Ouchi T,Howitt J,Tan SS


book

Compound heterozygous BRAT1 mutations cause familial Ohtahara syndrome with hypertonia and microcephaly.
Journal of human genetics59(12)687-90(2014 Dec)
Saitsu H,Yamashita S,Tanaka Y,Tsurusaki Y,Nakashima M,Miyake N,Matsumoto N


book

BRAT1 deficiency causes increased glucose metabolism and mitochondrial malfunction.
BMC cancer14548(2014 Jul)
So EY,Ouchi T


book

The Potential Role of BRCA1-Associated ATM Activator-1 (BRAT1) in Regulation of mTOR.
Journal of cancer biology & research1(1)(2013 Jul-Aug)
So EY,Ouchi T


 
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