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FGF8 cDNA ORF clone, Homo sapiens (human)

Gene Symbol FGF8
Entrez Gene ID 2253
Full Name fibroblast growth factor 8 (androgen-induced)
Synonyms AIGF, FGF-8, HBGF-8, HH6, KAL6
General protein information
Preferred Names
fibroblast growth factor 8
Names
fibroblast growth factor 8
androgen-induced growth factor
heparin-binding growth factor 8
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

10

10q24

Summary The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is known to be a factor that supports androgen and anchorage independent growth of mammary tumor cells. Overexpression of this gene has been shown to increase tumor growth and angiogensis. The adult expression of this gene is restricted to testes and ovaries. Temporal and spatial pattern of this gene expression suggests its function as an embryonic epithelial factor. Studies of the mouse and chick homologs revealed roles in midbrain and limb development, organogenesis, embryo gastrulation and left-right axis determination. The alternative splicing of this gene results in four transcript variants. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

600483

Disorder Html: Kallmann syndrome 6, 612702 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_011539509 XP_011537811 fibroblast growth factor 8 isoform X1
NM_001206389 NP_001193318 fibroblast growth factor 8 isoform G
NM_033164 NP_149354 fibroblast growth factor 8 isoform E precursor
NM_033165 NP_149355 fibroblast growth factor 8 isoform A precursor
NM_033163 NP_149353 fibroblast growth factor 8 isoform F precursor
NM_006119 NP_006110 fibroblast growth factor 8 isoform B precursor



Homo sapiens (human) FGF8 NP_149353.1
Bos taurus (cattle) FGF8 NP_001193607.1
Mus musculus (house mouse) Fgf8 NP_001159833.1
Rattus norvegicus (Norway rat) Fgf8 NP_579820.1
Gallus gallus (chicken) FGF8 NP_001012785.1
Danio rerio (zebrafish) fgf8a NP_571356.2
Xenopus (Silurana) tropicalis (western clawed frog) fgf8 NP_001008163.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following FGF8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FGF8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu57754 XM_011539509 PREDICTED: Homo sapiens fibroblast growth factor 8 (androgen-induced) (FGF8), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu23156 NM_001206389 Homo sapiens fibroblast growth factor 8 (androgen-induced) (FGF8), transcript variant G, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
OHu22066
NM_033164 Homo sapiens fibroblast growth factor 8 (androgen-induced) (FGF8), transcript variant E, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$134.50-$188.30
$269.00
OHu22083
NM_033165 Homo sapiens fibroblast growth factor 8 (androgen-induced) (FGF8), transcript variant A, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$134.50-$188.30
$269.00
OHu25132
NM_033163 Homo sapiens fibroblast growth factor 8 (androgen-induced) (FGF8), transcript variant F, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu22678
NM_006119 Homo sapiens fibroblast growth factor 8 (androgen-induced) (FGF8), transcript variant B, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu57754
Clone ID Related Accession (Same CDS sequence) XM_011539509
Accession Version XM_011539509.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 657bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product fibroblast growth factor 8 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
ATGCCACTGA TCGCTCCTTG CATTTCTCGC CGGTTTTCCG GGCTGTTGCA CTTGCTGGTC 
CTCTGCCTCC AAGCCCAGGT AACTGTTCAG TCCTCACCTA ATTTTACACA GCATGTGAGG
GAGCAGAGCC TGGTGACGGA TCAGCTCAGC CGCCGCCTCA TCCGGACCTA CCAACTCTAC
AGCCGCACCA GCGGGAAGCA CGTGCAGGTC CTGGCCAACA AGCGCATCAA CGCCATGGCA
GAGGACGGCG ACCCCTTCGC AAAGCTCATC GTGGAGACGG ACACCTTTGG AAGCAGAGTT
CGAGTCCGAG GAGCCGAGAC GGGCCTCTAC ATCTGCATGA ACAAGAAGGG GAAGCTGATC
GCCAAGAGCA ACGGCAAAGG CAAGGACTGC GTCTTCACGG AGATTGTGCT GGAGAACAAC
TACACAGCGC TGCAGAATGC CAAGTACGAG GGCTGGTACA TGGCCTTCAC CCGCAAGGGC
CGGCCCCGCA AGGGCTCCAA GACGCGGCAG CACCAGCGTG AGGTCCACTT CATGAAGCGG
CTGCCCCGGG GCCACCACAC CACCGAGCAG AGCCTGCGCT TCGAGTTCCT CAACTACCCG
CCCTTCACGC GCAGCCTGCG CGGCAGCCAG AGGACTTGGG CCCCCGAGCC CCGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011537811.1
CDS5..661
Misc Feature(1)173..547(+)
Misc Feature(2)173..544(+)
Misc Feature(3)473..523(+)
Translation

Target ORF information:

RefSeq Version XM_011539509
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens fibroblast growth factor 8 (androgen-induced) (FGF8), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011539509

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGCCACTGA TCGCTCCTTG CATTTCTCGC CGGTTTTCCG GGCTGTTGCA CTTGCTGGTC 
CTCTGCCTCC AAGCCCAGGT AACTGTTCAG TCCTCACCTA ATTTTACACA GCATGTGAGG
GAGCAGAGCC TGGTGACGGA TCAGCTCAGC CGCCGCCTCA TCCGGACCTA CCAACTCTAC
AGCCGCACCA GCGGGAAGCA CGTGCAGGTC CTGGCCAACA AGCGCATCAA CGCCATGGCA
GAGGACGGCG ACCCCTTCGC AAAGCTCATC GTGGAGACGG ACACCTTTGG AAGCAGAGTT
CGAGTCCGAG GAGCCGAGAC GGGCCTCTAC ATCTGCATGA ACAAGAAGGG GAAGCTGATC
GCCAAGAGCA ACGGCAAAGG CAAGGACTGC GTCTTCACGG AGATTGTGCT GGAGAACAAC
TACACAGCGC TGCAGAATGC CAAGTACGAG GGCTGGTACA TGGCCTTCAC CCGCAAGGGC
CGGCCCCGCA AGGGCTCCAA GACGCGGCAG CACCAGCGTG AGGTCCACTT CATGAAGCGG
CTGCCCCGGG GCCACCACAC CACCGAGCAG AGCCTGCGCT TCGAGTTCCT CAACTACCCG
CCCTTCACGC GCAGCCTGCG CGGCAGCCAG AGGACTTGGG CCCCCGAGCC CCGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23156
Clone ID Related Accession (Same CDS sequence) NM_001206389
Accession Version NM_001206389.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 423bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product fibroblast growth factor 8 isoform G
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN369230.1 and AC010789.9. Transcript Variant: This variant (G) has an alternate 5' exon and lacks an internal segment in the 5' region, compared to variant F. The encoded isoform (G) is shorter at the N-terminus, compared to isoform F. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CN369230.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2154665 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
ATGGCAGAGG ACGGCGACCC CTTCGCAAAG CTCATCGTGG AGACGGACAC CTTTGGAAGC 
AGAGTTCGAG TCCGAGGAGC CGAGACGGGC CTCTACATCT GCATGAACAA GAAGGGGAAG
CTGATCGCCA AGAGCAACGG CAAAGGCAAG GACTGCGTCT TCACGGAGAT TGTGCTGGAG
AACAACTACA CAGCGCTGCA GAATGCCAAG TACGAGGGCT GGTACATGGC CTTCACCCGC
AAGGGCCGGC CCCGCAAGGG CTCCAAGACG CGGCAGCACC AGCGTGAGGT CCACTTCATG
AAGCGGCTGC CCCGGGGCCA CCACACCACC GAGCAGAGCC TGCGCTTCGA GTTCCTCAAC
TACCCGCCCT TCACGCGCAG CCTGCGCGGC AGCCAGAGGA CTTGGGCCCC CGAGCCCCGA
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001193318.1
CDS235..657
Misc Feature(1)49..51(+)
Misc Feature(2)235..543(+)
Misc Feature(3)274..540(+)
Misc Feature(4)469..519(+)
Exon (1)1..74
Gene:FGF8
Gene Synonym:
Exon (2)75..111
Gene:FGF8
Gene Synonym:
Exon (3)112..259
Gene:FGF8
Gene Synonym:
Exon (4)260..366
Gene:FGF8
Gene Synonym:
Exon (5)367..856
Gene:FGF8
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001206389
Organism Homo sapiens (human)
Definition Homo sapiens fibroblast growth factor 8 (androgen-induced) (FGF8), transcript variant G, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001206389

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGCAGAGG ACGGCGACCC CTTCGCAAAG CTCATCGTGG AGACGGACAC CTTTGGAAGC 
AGAGTTCGAG TCCGAGGAGC CGAGACGGGC CTCTACATCT GCATGAACAA GAAGGGGAAG
CTGATCGCCA AGAGCAACGG CAAAGGCAAG GACTGCGTCT TCACGGAGAT TGTGCTGGAG
AACAACTACA CAGCGCTGCA GAATGCCAAG TACGAGGGCT GGTACATGGC CTTCACCCGC
AAGGGCCGGC CCCGCAAGGG CTCCAAGACG CGGCAGCACC AGCGTGAGGT CCACTTCATG
AAGCGGCTGC CCCGGGGCCA CCACACCACC GAGCAGAGCC TGCGCTTCGA GTTCCTCAAC
TACCCGCCCT TCACGCGCAG CCTGCGCGGC AGCCAGAGGA CTTGGGCCCC CGAGCCCCGA
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22066
Clone ID Related Accession (Same CDS sequence) NM_033164
Accession Version NM_033164.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 702bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product fibroblast growth factor 8 isoform E precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC128236.1, U46213.1 and AC010789.9. On Jun 22, 2010 this sequence version replaced gi:170763473. Transcript Variant: This variant (E) uses an alternate splice site, compared to variant F. The encoded isoform (E) is shorter than isoform F. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U46213.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2144333, SAMEA2145743 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGGGCAGCC CCCGCTCCGC GCTGAGCTGC CTGCTGTTGC ACTTGCTGGT CCTCTGCCTC 
CAAGCCCAGG AAGGCCCGGG CAGGGGCCCT GCGCTGGGCA GGGAGCTCGC TTCCCTGTTC
CGGGCTGGCC GGGAGCCCCA GGGTGTCTCC CAACAGCATG TGAGGGAGCA GAGCCTGGTG
ACGGATCAGC TCAGCCGCCG CCTCATCCGG ACCTACCAAC TCTACAGCCG CACCAGCGGG
AAGCACGTGC AGGTCCTGGC CAACAAGCGC ATCAACGCCA TGGCAGAGGA CGGCGACCCC
TTCGCAAAGC TCATCGTGGA GACGGACACC TTTGGAAGCA GAGTTCGAGT CCGAGGAGCC
GAGACGGGCC TCTACATCTG CATGAACAAG AAGGGGAAGC TGATCGCCAA GAGCAACGGC
AAAGGCAAGG ACTGCGTCTT CACGGAGATT GTGCTGGAGA ACAACTACAC AGCGCTGCAG
AATGCCAAGT ACGAGGGCTG GTACATGGCC TTCACCCGCA AGGGCCGGCC CCGCAAGGGC
TCCAAGACGC GGCAGCACCA GCGTGAGGTC CACTTCATGA AGCGGCTGCC CCGGGGCCAC
CACACCACCG AGCAGAGCCT GCGCTTCGAG TTCCTCAACT ACCCGCCCTT CACGCGCAGC
CTGCGCGGCA GCCAGAGGAC TTGGGCCCCC GAGCCCCGAT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_149354.1
CDS103..804
Misc Feature(1)316..690(+)
Misc Feature(2)316..687(+)
Misc Feature(3)616..666(+)
Exon (1)1..134
Gene:FGF8
Gene Synonym:
Exon (2)135..171
Gene:FGF8
Gene Synonym:
Exon (3)172..258
Gene:FGF8
Gene Synonym:
Exon (4)259..406
Gene:FGF8
Gene Synonym:
Exon (5)407..513
Gene:FGF8
Gene Synonym:
Exon (6)514..1003
Gene:FGF8
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_033164
Organism Homo sapiens (human)
Definition Homo sapiens fibroblast growth factor 8 (androgen-induced) (FGF8), transcript variant E, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_033164

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGGCAGCC CCCGCTCCGC GCTGAGCTGC CTGCTGTTGC ACTTGCTGGT CCTCTGCCTC 
CAAGCCCAGG AAGGCCCGGG CAGGGGCCCT GCGCTGGGCA GGGAGCTCGC TTCCCTGTTC
CGGGCTGGCC GGGAGCCCCA GGGTGTCTCC CAACAGCATG TGAGGGAGCA GAGCCTGGTG
ACGGATCAGC TCAGCCGCCG CCTCATCCGG ACCTACCAAC TCTACAGCCG CACCAGCGGG
AAGCACGTGC AGGTCCTGGC CAACAAGCGC ATCAACGCCA TGGCAGAGGA CGGCGACCCC
TTCGCAAAGC TCATCGTGGA GACGGACACC TTTGGAAGCA GAGTTCGAGT CCGAGGAGCC
GAGACGGGCC TCTACATCTG CATGAACAAG AAGGGGAAGC TGATCGCCAA GAGCAACGGC
AAAGGCAAGG ACTGCGTCTT CACGGAGATT GTGCTGGAGA ACAACTACAC AGCGCTGCAG
AATGCCAAGT ACGAGGGCTG GTACATGGCC TTCACCCGCA AGGGCCGGCC CCGCAAGGGC
TCCAAGACGC GGCAGCACCA GCGTGAGGTC CACTTCATGA AGCGGCTGCC CCGGGGCCAC
CACACCACCG AGCAGAGCCT GCGCTTCGAG TTCCTCAACT ACCCGCCCTT CACGCGCAGC
CTGCGCGGCA GCCAGAGGAC TTGGGCCCCC GAGCCCCGAT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22083
Clone ID Related Accession (Same CDS sequence) NM_033165
Accession Version NM_033165.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 615bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product fibroblast growth factor 8 isoform A precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC128236.1 and AC010789.9. On Jun 22, 2010 this sequence version replaced gi:170763475. Transcript Variant: This variant (A) lacks an in-frame exon and uses an alternate splice site, compared to variant F. The encoded isoform (A) is shorter than isoform F. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC128236.1, U46211.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154665 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
ATGGGCAGCC CCCGCTCCGC GCTGAGCTGC CTGCTGTTGC ACTTGCTGGT CCTCTGCCTC 
CAAGCCCAGC ATGTGAGGGA GCAGAGCCTG GTGACGGATC AGCTCAGCCG CCGCCTCATC
CGGACCTACC AACTCTACAG CCGCACCAGC GGGAAGCACG TGCAGGTCCT GGCCAACAAG
CGCATCAACG CCATGGCAGA GGACGGCGAC CCCTTCGCAA AGCTCATCGT GGAGACGGAC
ACCTTTGGAA GCAGAGTTCG AGTCCGAGGA GCCGAGACGG GCCTCTACAT CTGCATGAAC
AAGAAGGGGA AGCTGATCGC CAAGAGCAAC GGCAAAGGCA AGGACTGCGT CTTCACGGAG
ATTGTGCTGG AGAACAACTA CACAGCGCTG CAGAATGCCA AGTACGAGGG CTGGTACATG
GCCTTCACCC GCAAGGGCCG GCCCCGCAAG GGCTCCAAGA CGCGGCAGCA CCAGCGTGAG
GTCCACTTCA TGAAGCGGCT GCCCCGGGGC CACCACACCA CCGAGCAGAG CCTGCGCTTC
GAGTTCCTCA ACTACCCGCC CTTCACGCGC AGCCTGCGCG GCAGCCAGAG GACTTGGGCC
CCCGAGCCCC GATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_149355.1
CDS103..717
Misc Feature(1)229..603(+)
Misc Feature(2)229..600(+)
Misc Feature(3)529..579(+)
Exon (1)1..134
Gene:FGF8
Gene Synonym:
Exon (2)135..171
Gene:FGF8
Gene Synonym:
Exon (3)172..319
Gene:FGF8
Gene Synonym:
Exon (4)320..426
Gene:FGF8
Gene Synonym:
Exon (5)427..916
Gene:FGF8
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_033165
Organism Homo sapiens (human)
Definition Homo sapiens fibroblast growth factor 8 (androgen-induced) (FGF8), transcript variant A, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_033165

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGGCAGCC CCCGCTCCGC GCTGAGCTGC CTGCTGTTGC ACTTGCTGGT CCTCTGCCTC 
CAAGCCCAGC ATGTGAGGGA GCAGAGCCTG GTGACGGATC AGCTCAGCCG CCGCCTCATC
CGGACCTACC AACTCTACAG CCGCACCAGC GGGAAGCACG TGCAGGTCCT GGCCAACAAG
CGCATCAACG CCATGGCAGA GGACGGCGAC CCCTTCGCAA AGCTCATCGT GGAGACGGAC
ACCTTTGGAA GCAGAGTTCG AGTCCGAGGA GCCGAGACGG GCCTCTACAT CTGCATGAAC
AAGAAGGGGA AGCTGATCGC CAAGAGCAAC GGCAAAGGCA AGGACTGCGT CTTCACGGAG
ATTGTGCTGG AGAACAACTA CACAGCGCTG CAGAATGCCA AGTACGAGGG CTGGTACATG
GCCTTCACCC GCAAGGGCCG GCCCCGCAAG GGCTCCAAGA CGCGGCAGCA CCAGCGTGAG
GTCCACTTCA TGAAGCGGCT GCCCCGGGGC CACCACACCA CCGAGCAGAG CCTGCGCTTC
GAGTTCCTCA ACTACCCGCC CTTCACGCGC AGCCTGCGCG GCAGCCAGAG GACTTGGGCC
CCCGAGCCCC GATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25132
Clone ID Related Accession (Same CDS sequence) NM_033163
Accession Version NM_033163.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 735bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product fibroblast growth factor 8 isoform F precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC128236.1, BC069106.1 and AC010789.9. This sequence is a reference standard in the RefSeqGene project. On Jun 22, 2010 this sequence version replaced gi:170763468. Transcript Variant: This variant (F) encodes the longest isoform (F). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC069106.1, AB014615.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2142680, SAMEA2144333 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGGCAGCC CCCGCTCCGC GCTGAGCTGC CTGCTGTTGC ACTTGCTGGT CCTCTGCCTC 
CAAGCCCAGG AAGGCCCGGG CAGGGGCCCT GCGCTGGGCA GGGAGCTCGC TTCCCTGTTC
CGGGCTGGCC GGGAGCCCCA GGGTGTCTCC CAACAGGTAA CTGTTCAGTC CTCACCTAAT
TTTACACAGC ATGTGAGGGA GCAGAGCCTG GTGACGGATC AGCTCAGCCG CCGCCTCATC
CGGACCTACC AACTCTACAG CCGCACCAGC GGGAAGCACG TGCAGGTCCT GGCCAACAAG
CGCATCAACG CCATGGCAGA GGACGGCGAC CCCTTCGCAA AGCTCATCGT GGAGACGGAC
ACCTTTGGAA GCAGAGTTCG AGTCCGAGGA GCCGAGACGG GCCTCTACAT CTGCATGAAC
AAGAAGGGGA AGCTGATCGC CAAGAGCAAC GGCAAAGGCA AGGACTGCGT CTTCACGGAG
ATTGTGCTGG AGAACAACTA CACAGCGCTG CAGAATGCCA AGTACGAGGG CTGGTACATG
GCCTTCACCC GCAAGGGCCG GCCCCGCAAG GGCTCCAAGA CGCGGCAGCA CCAGCGTGAG
GTCCACTTCA TGAAGCGGCT GCCCCGGGGC CACCACACCA CCGAGCAGAG CCTGCGCTTC
GAGTTCCTCA ACTACCCGCC CTTCACGCGC AGCCTGCGCG GCAGCCAGAG GACTTGGGCC
CCCGAGCCCC GATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_149353.1
CDS103..837
Misc Feature(1)349..723(+)
Misc Feature(2)349..720(+)
Misc Feature(3)649..699(+)
Exon (1)1..134
Gene:FGF8
Gene Synonym:
Exon (2)135..171
Gene:FGF8
Gene Synonym:
Exon (3)172..258
Gene:FGF8
Gene Synonym:
Exon (4)259..439
Gene:FGF8
Gene Synonym:
Exon (5)440..546
Gene:FGF8
Gene Synonym:
Exon (6)547..1036
Gene:FGF8
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_033163
Organism Homo sapiens (human)
Definition Homo sapiens fibroblast growth factor 8 (androgen-induced) (FGF8), transcript variant F, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_033163

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGGCAGCC CCCGCTCCGC GCTGAGCTGC CTGCTGTTGC ACTTGCTGGT CCTCTGCCTC 
CAAGCCCAGG AAGGCCCGGG CAGGGGCCCT GCGCTGGGCA GGGAGCTCGC TTCCCTGTTC
CGGGCTGGCC GGGAGCCCCA GGGTGTCTCC CAACAGGTAA CTGTTCAGTC CTCACCTAAT
TTTACACAGC ATGTGAGGGA GCAGAGCCTG GTGACGGATC AGCTCAGCCG CCGCCTCATC
CGGACCTACC AACTCTACAG CCGCACCAGC GGGAAGCACG TGCAGGTCCT GGCCAACAAG
CGCATCAACG CCATGGCAGA GGACGGCGAC CCCTTCGCAA AGCTCATCGT GGAGACGGAC
ACCTTTGGAA GCAGAGTTCG AGTCCGAGGA GCCGAGACGG GCCTCTACAT CTGCATGAAC
AAGAAGGGGA AGCTGATCGC CAAGAGCAAC GGCAAAGGCA AGGACTGCGT CTTCACGGAG
ATTGTGCTGG AGAACAACTA CACAGCGCTG CAGAATGCCA AGTACGAGGG CTGGTACATG
GCCTTCACCC GCAAGGGCCG GCCCCGCAAG GGCTCCAAGA CGCGGCAGCA CCAGCGTGAG
GTCCACTTCA TGAAGCGGCT GCCCCGGGGC CACCACACCA CCGAGCAGAG CCTGCGCTTC
GAGTTCCTCA ACTACCCGCC CTTCACGCGC AGCCTGCGCG GCAGCCAGAG GACTTGGGCC
CCCGAGCCCC GATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22678
Clone ID Related Accession (Same CDS sequence) NM_006119
Accession Version NM_006119.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 648bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product fibroblast growth factor 8 isoform B precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC128236.1, U36223.1 and AC010789.9. On Jun 22, 2010 this sequence version replaced gi:170763474. Transcript Variant: This variant (B) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (B) is shorter than isoform F. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U36223.1, U46212.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2144333, SAMEA2145743 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
ATGGGCAGCC CCCGCTCCGC GCTGAGCTGC CTGCTGTTGC ACTTGCTGGT CCTCTGCCTC 
CAAGCCCAGG TAACTGTTCA GTCCTCACCT AATTTTACAC AGCATGTGAG GGAGCAGAGC
CTGGTGACGG ATCAGCTCAG CCGCCGCCTC ATCCGGACCT ACCAACTCTA CAGCCGCACC
AGCGGGAAGC ACGTGCAGGT CCTGGCCAAC AAGCGCATCA ACGCCATGGC AGAGGACGGC
GACCCCTTCG CAAAGCTCAT CGTGGAGACG GACACCTTTG GAAGCAGAGT TCGAGTCCGA
GGAGCCGAGA CGGGCCTCTA CATCTGCATG AACAAGAAGG GGAAGCTGAT CGCCAAGAGC
AACGGCAAAG GCAAGGACTG CGTCTTCACG GAGATTGTGC TGGAGAACAA CTACACAGCG
CTGCAGAATG CCAAGTACGA GGGCTGGTAC ATGGCCTTCA CCCGCAAGGG CCGGCCCCGC
AAGGGCTCCA AGACGCGGCA GCACCAGCGT GAGGTCCACT TCATGAAGCG GCTGCCCCGG
GGCCACCACA CCACCGAGCA GAGCCTGCGC TTCGAGTTCC TCAACTACCC GCCCTTCACG
CGCAGCCTGC GCGGCAGCCA GAGGACTTGG GCCCCCGAGC CCCGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_006110.1
CDS103..750
Misc Feature(1)262..636(+)
Misc Feature(2)262..633(+)
Misc Feature(3)562..612(+)
Exon (1)1..134
Gene:FGF8
Gene Synonym:
Exon (2)135..171
Gene:FGF8
Gene Synonym:
Exon (3)172..352
Gene:FGF8
Gene Synonym:
Exon (4)353..459
Gene:FGF8
Gene Synonym:
Exon (5)460..949
Gene:FGF8
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_006119
Organism Homo sapiens (human)
Definition Homo sapiens fibroblast growth factor 8 (androgen-induced) (FGF8), transcript variant B, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006119

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGGCAGCC CCCGCTCCGC GCTGAGCTGC CTGCTGTTGC ACTTGCTGGT CCTCTGCCTC 
CAAGCCCAGG TAACTGTTCA GTCCTCACCT AATTTTACAC AGCATGTGAG GGAGCAGAGC
CTGGTGACGG ATCAGCTCAG CCGCCGCCTC ATCCGGACCT ACCAACTCTA CAGCCGCACC
AGCGGGAAGC ACGTGCAGGT CCTGGCCAAC AAGCGCATCA ACGCCATGGC AGAGGACGGC
GACCCCTTCG CAAAGCTCAT CGTGGAGACG GACACCTTTG GAAGCAGAGT TCGAGTCCGA
GGAGCCGAGA CGGGCCTCTA CATCTGCATG AACAAGAAGG GGAAGCTGAT CGCCAAGAGC
AACGGCAAAG GCAAGGACTG CGTCTTCACG GAGATTGTGC TGGAGAACAA CTACACAGCG
CTGCAGAATG CCAAGTACGA GGGCTGGTAC ATGGCCTTCA CCCGCAAGGG CCGGCCCCGC
AAGGGCTCCA AGACGCGGCA GCACCAGCGT GAGGTCCACT TCATGAAGCG GCTGCCCCGG
GGCCACCACA CCACCGAGCA GAGCCTGCGC TTCGAGTTCC TCAACTACCC GCCCTTCACG
CGCAGCCTGC GCGGCAGCCA GAGGACTTGG GCCCCCGAGC CCCGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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