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FHL1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol FHL1
Entrez Gene ID 2273
Full Name four and a half LIM domains 1
Synonyms FHL-1, FHL1A, FHL1B, FLH1A, KYOT, SLIM, SLIM-1, SLIM1, SLIMMER, XMPMA
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xq26

Summary This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.[provided by RefSeq, Nov 2009]. lac of sum
Disorder MIM:

300163

Disorder Html: Scapuloperoneal myopathy, X-linked dominant, 300695 (3); Myopathy,

mRNA and Protein(s)

mRNA Protein Name
NM_001159699 NP_001153171 four and a half LIM domains protein 1 isoform 5
NM_001159700 NP_001153172 four and a half LIM domains protein 1 isoform 2
NM_001159701 NP_001153173 four and a half LIM domains protein 1 isoform 3 precursor
NM_001159702 NP_001153174 four and a half LIM domains protein 1 isoform 1
NM_001159703 NP_001153175 four and a half LIM domains protein 1 isoform 4
NM_001159704 NP_001153176 four and a half LIM domains protein 1 isoform 2
NM_001167819 NP_001161291 four and a half LIM domains protein 1 isoform 2
NM_001449 NP_001440 four and a half LIM domains protein 1 isoform 2
XM_006724743 XP_006724806 four and a half LIM domains protein 1 isoform X3
XM_006724744 XP_006724807 four and a half LIM domains protein 1 isoform X2
XM_006724745 XP_006724808 four and a half LIM domains protein 1 isoform X2
XM_006724746 XP_006724809 four and a half LIM domains protein 1 isoform X2
XM_006724747 XP_006724810 four and a half LIM domains protein 1 isoform X1
XM_011531316 XP_011529618 four and a half LIM domains protein 1 isoform X4



Homo sapiens (human) FHL1 NP_001153173.1
Macaca mulatta (Rhesus monkey) FHL1 XP_002806455.1
Canis lupus familiaris (dog) FHL1 XP_866275.2
Bos taurus (cattle) FHL1 NP_001106729.1
Mus musculus (house mouse) Fhl1 NP_001070830.1
Rattus norvegicus (Norway rat) Fhl1 NP_001029098.1
Gallus gallus (chicken) FHL1 XP_001234114.2
Danio rerio (zebrafish) fhl1a NP_001007288.1
Xenopus (Silurana) tropicalis (western clawed frog) fhl1 NP_001006703.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following FHL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FHL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu16596 NM_001159699 Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $216.30
$309.00
OHu16400
NM_001159700 Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu16541 NM_001159701 Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $216.30
$309.00
OHu19535 NM_001159702 Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu21007 NM_001159703 Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu16400
NM_001159704 Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu16400
NM_001167819 Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant 9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu16400
NM_001449 Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu54313 XM_006724743 PREDICTED: Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu19535 XM_006724744 PREDICTED: Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu19535 XM_006724745 PREDICTED: Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu19535 XM_006724746 PREDICTED: Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu16400
XM_006724747 PREDICTED: Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu57783 XM_011531316 PREDICTED: Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu16596
Clone ID Related Accession (Same CDS sequence) NM_001159699
Accession Version NM_001159699.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 891bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product four and a half LIM domains protein 1 isoform 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC373386.1, BC088369.1 and BM995585.1. Transcript Variant: This variant (7) differs in the 5' UTR, 3' UTR, and 3' coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded protein (isoform 5) has distinct N- and C-termini and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC088369.1, AK299381.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCTTCCC ATAGACACTC AGGTCCCTCC AGCTACAAGG TGGGCACCAT GGCGGAGAAG 
TTTGACTGCC ACTACTGCAG GGATCCCTTG CAGGGGAAGA AGTATGTGCA AAAGGATGGC
CACCACTGCT GCCTGAAATG CTTTGACAAG TTCTGTGCCA ACACCTGTGT GGAATGCCGC
AAGCCCATCG GTGCGGACTC CAAGGAGGTG CACTATAAGA ACCGCTTCTG GCATGACACC
TGCTTCCGCT GTGCCAAGTG CCTTCACCCC TTGGCCAATG AGACCTTTGT GGCCAAGGAC
AACAAGATCC TGTGCAACAA GTGCACCACT CGGGAGGACT CCCCCAAGTG CAAGGGGTGC
TTCAAGGCCA TTGTGGCAGG AGATCAAAAC GTGGAGTACA AGGGGACCGT CTGGCACAAA
GACTGCTTCA CCTGTAGTAA CTGCAAGCAA GTCATCGGGA CTGGAAGCTT CTTCCCTAAA
GGGGAGGACT TCTACTGCGT GACTTGCCAT GAGACCAAGT TTGCCAAGCA TTGCGTGAAG
TGCAACAAGG CCATCACATC TGGAGGAATC ACTTACCAGG ATCAGCCCTG GCATGCCGAT
TGCTTTGTGT GTGTTACCTG CTCTAAGAAG CTGGCTGGGC AGCGTTTCAC CGCTGTGGAG
GACCAGTATT ACTGCGTGGA TTGCTACAAG AACTTTGTGG CCAAGAAGTG TGCTGGATGC
AAGAACCCCA TCACTGGGTT TGGTAAAGGC TCCAGTGTGG TGGCCTATGA AGGACAATCC
TGGCACGACT ACTGCTTCCA CTGCAAAAAA TGCTCCGTGA ATCTGGCCAA CAAGCGCTTT
GTTTTCCACC AGGAGCAAGT GTATTGTCCC GACTGTGCCA AAAAGCTGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001153171.1
CDS218..1108
Misc Feature(1)212..214(+)
Misc Feature(2)278..364(+)
Misc Feature(3)284..358(+)
Misc Feature(4)383..544(+)
Misc Feature(5)383..541(+)
Misc Feature(6)566..739(+)
Misc Feature(7)566..724(+)
Misc Feature(8)749..907(+)
Misc Feature(9)749..901(+)
Misc Feature(10)914..1105(+)
Misc Feature(11)926..1093(+)
Exon (1)1..239
Gene:FHL1
Gene Synonym:
Exon (2)240..421
Gene:FHL1
Gene Synonym:
Exon (3)422..596
Gene:FHL1
Gene Synonym:
Exon (4)597..766
Gene:FHL1
Gene Synonym:
Exon (5)767..953
Gene:FHL1
Gene Synonym:
Exon (6)954..2442
Gene:FHL1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001159699
Organism Homo sapiens (human)
Definition Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant 7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001159699

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCTTCCC ATAGACACTC AGGTCCCTCC AGCTACAAGG TGGGCACCAT GGCGGAGAAG 
TTTGACTGCC ACTACTGCAG GGATCCCTTG CAGGGGAAGA AGTATGTGCA AAAGGATGGC
CACCACTGCT GCCTGAAATG CTTTGACAAG TTCTGTGCCA ACACCTGTGT GGAATGCCGC
AAGCCCATCG GTGCGGACTC CAAGGAGGTG CACTATAAGA ACCGCTTCTG GCATGACACC
TGCTTCCGCT GTGCCAAGTG CCTTCACCCC TTGGCCAATG AGACCTTTGT GGCCAAGGAC
AACAAGATCC TGTGCAACAA GTGCACCACT CGGGAGGACT CCCCCAAGTG CAAGGGGTGC
TTCAAGGCCA TTGTGGCAGG AGATCAAAAC GTGGAGTACA AGGGGACCGT CTGGCACAAA
GACTGCTTCA CCTGTAGTAA CTGCAAGCAA GTCATCGGGA CTGGAAGCTT CTTCCCTAAA
GGGGAGGACT TCTACTGCGT GACTTGCCAT GAGACCAAGT TTGCCAAGCA TTGCGTGAAG
TGCAACAAGG CCATCACATC TGGAGGAATC ACTTACCAGG ATCAGCCCTG GCATGCCGAT
TGCTTTGTGT GTGTTACCTG CTCTAAGAAG CTGGCTGGGC AGCGTTTCAC CGCTGTGGAG
GACCAGTATT ACTGCGTGGA TTGCTACAAG AACTTTGTGG CCAAGAAGTG TGCTGGATGC
AAGAACCCCA TCACTGGGTT TGGTAAAGGC TCCAGTGTGG TGGCCTATGA AGGACAATCC
TGGCACGACT ACTGCTTCCA CTGCAAAAAA TGCTCCGTGA ATCTGGCCAA CAAGCGCTTT
GTTTTCCACC AGGAGCAAGT GTATTGTCCC GACTGTGCCA AAAAGCTGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16400
Clone ID Related Accession (Same CDS sequence) NM_001159700 , NM_001159704 , NM_001167819 , NM_001449 , XM_006724747
Accession Version NM_001159700.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 843bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product four and a half LIM domains protein 1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC363191.1, AK304680.1, AL078638.9, BC088369.1 and BM995585.1. Transcript Variant: This variant (3) differs in the 5' UTR, 3' UTR, and 3' coding region compared to variant 1. The encoded protein (isoform 2) has a distinct C-terminus and is shorter than isoform 1. Variants 2, 3, 4 and 9 encode the same protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304680.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCGGAGA AGTTTGACTG CCACTACTGC AGGGATCCCT TGCAGGGGAA GAAGTATGTG 
CAAAAGGATG GCCACCACTG CTGCCTGAAA TGCTTTGACA AGTTCTGTGC CAACACCTGT
GTGGAATGCC GCAAGCCCAT CGGTGCGGAC TCCAAGGAGG TGCACTATAA GAACCGCTTC
TGGCATGACA CCTGCTTCCG CTGTGCCAAG TGCCTTCACC CCTTGGCCAA TGAGACCTTT
GTGGCCAAGG ACAACAAGAT CCTGTGCAAC AAGTGCACCA CTCGGGAGGA CTCCCCCAAG
TGCAAGGGGT GCTTCAAGGC CATTGTGGCA GGAGATCAAA ACGTGGAGTA CAAGGGGACC
GTCTGGCACA AAGACTGCTT CACCTGTAGT AACTGCAAGC AAGTCATCGG GACTGGAAGC
TTCTTCCCTA AAGGGGAGGA CTTCTACTGC GTGACTTGCC ATGAGACCAA GTTTGCCAAG
CATTGCGTGA AGTGCAACAA GGCCATCACA TCTGGAGGAA TCACTTACCA GGATCAGCCC
TGGCATGCCG ATTGCTTTGT GTGTGTTACC TGCTCTAAGA AGCTGGCTGG GCAGCGTTTC
ACCGCTGTGG AGGACCAGTA TTACTGCGTG GATTGCTACA AGAACTTTGT GGCCAAGAAG
TGTGCTGGAT GCAAGAACCC CATCACTGGG TTTGGTAAAG GCTCCAGTGT GGTGGCCTAT
GAAGGACAAT CCTGGCACGA CTACTGCTTC CACTGCAAAA AATGCTCCGT GAATCTGGCC
AACAAGCGCT TTGTTTTCCA CCAGGAGCAA GTGTATTGTC CCGACTGTGC CAAAAAGCTG
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001153172.1
CDS261..1103
Misc Feature(1)231..233(+)
Misc Feature(2)273..359(+)
Misc Feature(3)279..353(+)
Misc Feature(4)378..539(+)
Misc Feature(5)378..536(+)
Misc Feature(6)561..734(+)
Misc Feature(7)561..719(+)
Misc Feature(8)744..902(+)
Misc Feature(9)744..896(+)
Misc Feature(10)909..1100(+)
Misc Feature(11)921..1088(+)
Exon (1)1..160
Gene:FHL1
Gene Synonym:
Exon (2)161..234
Gene:FHL1
Gene Synonym:
Exon (3)235..416
Gene:FHL1
Gene Synonym:
Exon (4)417..591
Gene:FHL1
Gene Synonym:
Exon (5)592..761
Gene:FHL1
Gene Synonym:
Exon (6)762..948
Gene:FHL1
Gene Synonym:
Exon (7)949..2437
Gene:FHL1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001159700
Organism Homo sapiens (human)
Definition Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001159700

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCGGAGA AGTTTGACTG CCACTACTGC AGGGATCCCT TGCAGGGGAA GAAGTATGTG 
CAAAAGGATG GCCACCACTG CTGCCTGAAA TGCTTTGACA AGTTCTGTGC CAACACCTGT
GTGGAATGCC GCAAGCCCAT CGGTGCGGAC TCCAAGGAGG TGCACTATAA GAACCGCTTC
TGGCATGACA CCTGCTTCCG CTGTGCCAAG TGCCTTCACC CCTTGGCCAA TGAGACCTTT
GTGGCCAAGG ACAACAAGAT CCTGTGCAAC AAGTGCACCA CTCGGGAGGA CTCCCCCAAG
TGCAAGGGGT GCTTCAAGGC CATTGTGGCA GGAGATCAAA ACGTGGAGTA CAAGGGGACC
GTCTGGCACA AAGACTGCTT CACCTGTAGT AACTGCAAGC AAGTCATCGG GACTGGAAGC
TTCTTCCCTA AAGGGGAGGA CTTCTACTGC GTGACTTGCC ATGAGACCAA GTTTGCCAAG
CATTGCGTGA AGTGCAACAA GGCCATCACA TCTGGAGGAA TCACTTACCA GGATCAGCCC
TGGCATGCCG ATTGCTTTGT GTGTGTTACC TGCTCTAAGA AGCTGGCTGG GCAGCGTTTC
ACCGCTGTGG AGGACCAGTA TTACTGCGTG GATTGCTACA AGAACTTTGT GGCCAAGAAG
TGTGCTGGAT GCAAGAACCC CATCACTGGG TTTGGTAAAG GCTCCAGTGT GGTGGCCTAT
GAAGGACAAT CCTGGCACGA CTACTGCTTC CACTGCAAAA AATGCTCCGT GAATCTGGCC
AACAAGCGCT TTGTTTTCCA CCAGGAGCAA GTGTATTGTC CCGACTGTGC CAAAAAGCTG
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16541
Clone ID Related Accession (Same CDS sequence) NM_001159701
Accession Version NM_001159701.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 930bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product four and a half LIM domains protein 1 isoform 3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC373304.1, AK301642.1, BC010998.2 and BM995585.1. Transcript Variant: This variant (5) differs in the 5' UTR, 3' UTR, and coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded protein (isoform 3) is shorter and has distinct N- and C-termini, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK301642.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGACTTTCT ATGTGGCCTC TCTGGCTCTG GAGCTAATTT GGATGCTGAG TAGCCCCGCA 
GGTCCCTCCA GCTACAAGGT GGGCACCATG GCGGAGAAGT TTGACTGCCA CTACTGCAGG
GATCCCTTGC AGGGGAAGAA GTATGTGCAA AAGGATGGCC ACCACTGCTG CCTGAAATGC
TTTGACAAGT TCTGTGCCAA CACCTGTGTG GAATGCCGCA AGCCCATCGG TGCGGACTCC
AAGGAGGTGC ACTATAAGAA CCGCTTCTGG CATGACACCT GCTTCCGCTG TGCCAAGTGC
CTTCACCCCT TGGCCAATGA GACCTTTGTG GCCAAGGACA ACAAGATCCT GTGCAACAAG
TGCACCACTC GGGAGGACTC CCCCAAGTGC AAGGGGTGCT TCAAGGCCAT TGTGGCAGGA
GATCAAAACG TGGAGTACAA GGGGACCGTC TGGCACAAAG ACTGCTTCAC CTGTAGTAAC
TGCAAGCAAG TCATCGGGAC TGGAAGCTTC TTCCCTAAAG GGGAGGACTT CTACTGCGTG
ACTTGCCATG AGACCAAGTT TGCCAAGCAT TGCGTGAAGT GCAACAAGGC CATCACATCT
GGAGGAATCA CTTACCAGGA TCAGCCCTGG CATGCCGATT GCTTTGTGTG TGTTACCTGC
TCTAAGAAGC TGGCTGGGCA GCGTTTCACC GCTGTGGAGG ACCAGTATTA CTGCGTGGAT
TGCTACAAGA ACTTTGTGGC CAAGAAGTGT GCTGGATGCA AGAACCCCAT CACTGGGTTT
GGTAAAGGCT CCAGTGTGGT GGCCTATGAA GGACAATCCT GGCACGACTA CTGCTTCCAC
TGCAAAAAAT GCTCCGTGAA TCTGGCCAAC AAGCGCTTTG TTTTCCACCA GGAGCAAGTG
TATTGTCCCG ACTGTGCCAA AAAGCTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001153173.1
CDS50..979
Misc Feature(1)149..235(+)
Misc Feature(2)155..229(+)
Misc Feature(3)254..415(+)
Misc Feature(4)254..412(+)
Misc Feature(5)437..610(+)
Misc Feature(6)437..595(+)
Misc Feature(7)620..778(+)
Misc Feature(8)620..772(+)
Misc Feature(9)785..976(+)
Misc Feature(10)797..964(+)
Exon (1)1..110
Gene:FHL1
Gene Synonym:
Exon (2)111..292
Gene:FHL1
Gene Synonym:
Exon (3)293..467
Gene:FHL1
Gene Synonym:
Exon (4)468..637
Gene:FHL1
Gene Synonym:
Exon (5)638..824
Gene:FHL1
Gene Synonym:
Exon (6)825..2313
Gene:FHL1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001159701
Organism Homo sapiens (human)
Definition Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001159701

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGACTTTCT ATGTGGCCTC TCTGGCTCTG GAGCTAATTT GGATGCTGAG TAGCCCCGCA 
GGTCCCTCCA GCTACAAGGT GGGCACCATG GCGGAGAAGT TTGACTGCCA CTACTGCAGG
GATCCCTTGC AGGGGAAGAA GTATGTGCAA AAGGATGGCC ACCACTGCTG CCTGAAATGC
TTTGACAAGT TCTGTGCCAA CACCTGTGTG GAATGCCGCA AGCCCATCGG TGCGGACTCC
AAGGAGGTGC ACTATAAGAA CCGCTTCTGG CATGACACCT GCTTCCGCTG TGCCAAGTGC
CTTCACCCCT TGGCCAATGA GACCTTTGTG GCCAAGGACA ACAAGATCCT GTGCAACAAG
TGCACCACTC GGGAGGACTC CCCCAAGTGC AAGGGGTGCT TCAAGGCCAT TGTGGCAGGA
GATCAAAACG TGGAGTACAA GGGGACCGTC TGGCACAAAG ACTGCTTCAC CTGTAGTAAC
TGCAAGCAAG TCATCGGGAC TGGAAGCTTC TTCCCTAAAG GGGAGGACTT CTACTGCGTG
ACTTGCCATG AGACCAAGTT TGCCAAGCAT TGCGTGAAGT GCAACAAGGC CATCACATCT
GGAGGAATCA CTTACCAGGA TCAGCCCTGG CATGCCGATT GCTTTGTGTG TGTTACCTGC
TCTAAGAAGC TGGCTGGGCA GCGTTTCACC GCTGTGGAGG ACCAGTATTA CTGCGTGGAT
TGCTACAAGA ACTTTGTGGC CAAGAAGTGT GCTGGATGCA AGAACCCCAT CACTGGGTTT
GGTAAAGGCT CCAGTGTGGT GGCCTATGAA GGACAATCCT GGCACGACTA CTGCTTCCAC
TGCAAAAAAT GCTCCGTGAA TCTGGCCAAC AAGCGCTTTG TTTTCCACCA GGAGCAAGTG
TATTGTCCCG ACTGTGCCAA AAAGCTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19535
Clone ID Related Accession (Same CDS sequence) NM_001159702 , XM_006724744 , XM_006724745 , XM_006724746
Accession Version NM_001159702.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 972bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product four and a half LIM domains protein 1 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Valerie Delague, Johan den Dunnen, Isabelle Richard, James Ware. The reference sequence was derived from DA497183.1, BC010998.2, AF098518.1, AF063002.1 and BM995585.1. This sequence is a reference standard in the RefSeqGene project. On Nov 18, 2009 this sequence version replaced gi:228480210. Transcript Variant: This variant (1) encodes the longest protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF063002.1, AF098518.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCGGAGA AGTTTGACTG CCACTACTGC AGGGATCCCT TGCAGGGGAA GAAGTATGTG 
CAAAAGGATG GCCACCACTG CTGCCTGAAA TGCTTTGACA AGTTCTGTGC CAACACCTGT
GTGGAATGCC GCAAGCCCAT CGGTGCGGAC TCCAAGGAGG TGCACTATAA GAACCGCTTC
TGGCATGACA CCTGCTTCCG CTGTGCCAAG TGCCTTCACC CCTTGGCCAA TGAGACCTTT
GTGGCCAAGG ACAACAAGAT CCTGTGCAAC AAGTGCACCA CTCGGGAGGA CTCCCCCAAG
TGCAAGGGGT GCTTCAAGGC CATTGTGGCA GGAGATCAAA ACGTGGAGTA CAAGGGGACC
GTCTGGCACA AAGACTGCTT CACCTGTAGT AACTGCAAGC AAGTCATCGG GACTGGAAGC
TTCTTCCCTA AAGGGGAGGA CTTCTACTGC GTGACTTGCC ATGAGACCAA GTTTGCCAAG
CATTGCGTGA AGTGCAACAA GGCCATCACA TCTGGAGGAA TCACTTACCA GGATCAGCCC
TGGCATGCCG ATTGCTTTGT GTGTGTTACC TGCTCTAAGA AGCTGGCTGG GCAGCGTTTC
ACCGCTGTGG AGGACCAGTA TTACTGCGTG GATTGCTACA AGAACTTTGT GGCCAAGAAG
TGTGCTGGAT GCAAGAACCC CATCACTGGG AAAAGGACTG TGTCAAGAGT GAGCCACCCA
GTCTCTAAAG CTAGGAAGCC CCCAGTGTGC CACGGGAAAC GCTTGCCTCT CACCCTGTTT
CCCAGCGCCA ACCTCCGGGG CAGGCATCCG GGTGGAGAGA GGACTTGTCC CTCGTGGGTG
GTGGTTCTTT ATAGAAAAAA TCGAAGCTTA GCAGCTCCTC GAGGCCCGGG TTTGGTAAAG
GCTCCAGTGT GGTGGCCTAT GAAGGACAAT CCTGGCACGA CTACTGCTTC CACTGCAAAA
AATGCTCCGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001153174.1
CDS330..1301
Misc Feature(1)300..302(+)
Misc Feature(2)342..428(+)
Misc Feature(3)348..422(+)
Misc Feature(4)447..608(+)
Misc Feature(5)447..605(+)
Misc Feature(6)630..803(+)
Misc Feature(7)630..788(+)
Misc Feature(8)813..971(+)
Misc Feature(9)813..965(+)
Exon (1)1..229
Gene:FHL1
Gene Synonym:
Exon (2)230..303
Gene:FHL1
Gene Synonym:
Exon (3)304..485
Gene:FHL1
Gene Synonym:
Exon (4)486..660
Gene:FHL1
Gene Synonym:
Exon (5)661..830
Gene:FHL1
Gene Synonym:
Exon (6)831..1017
Gene:FHL1
Gene Synonym:
Exon (7)1018..1217
Gene:FHL1
Gene Synonym:
Exon (8)1218..2706
Gene:FHL1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001159702
Organism Homo sapiens (human)
Definition Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001159702

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCGGAGA AGTTTGACTG CCACTACTGC AGGGATCCCT TGCAGGGGAA GAAGTATGTG 
CAAAAGGATG GCCACCACTG CTGCCTGAAA TGCTTTGACA AGTTCTGTGC CAACACCTGT
GTGGAATGCC GCAAGCCCAT CGGTGCGGAC TCCAAGGAGG TGCACTATAA GAACCGCTTC
TGGCATGACA CCTGCTTCCG CTGTGCCAAG TGCCTTCACC CCTTGGCCAA TGAGACCTTT
GTGGCCAAGG ACAACAAGAT CCTGTGCAAC AAGTGCACCA CTCGGGAGGA CTCCCCCAAG
TGCAAGGGGT GCTTCAAGGC CATTGTGGCA GGAGATCAAA ACGTGGAGTA CAAGGGGACC
GTCTGGCACA AAGACTGCTT CACCTGTAGT AACTGCAAGC AAGTCATCGG GACTGGAAGC
TTCTTCCCTA AAGGGGAGGA CTTCTACTGC GTGACTTGCC ATGAGACCAA GTTTGCCAAG
CATTGCGTGA AGTGCAACAA GGCCATCACA TCTGGAGGAA TCACTTACCA GGATCAGCCC
TGGCATGCCG ATTGCTTTGT GTGTGTTACC TGCTCTAAGA AGCTGGCTGG GCAGCGTTTC
ACCGCTGTGG AGGACCAGTA TTACTGCGTG GATTGCTACA AGAACTTTGT GGCCAAGAAG
TGTGCTGGAT GCAAGAACCC CATCACTGGG AAAAGGACTG TGTCAAGAGT GAGCCACCCA
GTCTCTAAAG CTAGGAAGCC CCCAGTGTGC CACGGGAAAC GCTTGCCTCT CACCCTGTTT
CCCAGCGCCA ACCTCCGGGG CAGGCATCCG GGTGGAGAGA GGACTTGTCC CTCGTGGGTG
GTGGTTCTTT ATAGAAAAAA TCGAAGCTTA GCAGCTCCTC GAGGCCCGGG TTTGGTAAAG
GCTCCAGTGT GGTGGCCTAT GAAGGACAAT CCTGGCACGA CTACTGCTTC CACTGCAAAA
AATGCTCCGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21007
Clone ID Related Accession (Same CDS sequence) NM_001159703
Accession Version NM_001159703.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 585bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product four and a half LIM domains protein 1 isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA497183.1, AL078638.9, BE965488.2, AK301642.1, AF220153.1, BC010998.2 and BM995585.1. Transcript Variant: This variant (6) differs in the 5' UTR and coding region, compared to variant 1. The encoded protein (isoform 4) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BE965488.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGGCGGAGA AGTTTGACTG CCACTACTGC AGGGATCCCT TGCAGGGGAA GAAGTATGTG 
CAAAAGGATG GCCACCACTG CTGCCTGAAA TGCTTTGACA AGTTCTGTGC CAACACCTGT
GTGGAATGCC GCAAGCCCAT CGGTGCGGAC TCCAAGGAGG TGCACTATAA GAACCGCTTC
TGGCATGACA CCTGCTTCCG CTGTGCCAAG TGCCTTCACC CCTTGGCCAA TGAGACCTTT
GTGGCCAAGG ACAACAAGAT CCTGTGCAAC AAGTGCACCA CTCGGGAGGA CTCCCCCAAG
TGCAAGGGGT GCTTCAAGGC CATTGTGGCA GGAGATCAAA ACGTGGAGTA CAAGGGGACC
GTCTGGCACA AAGACTGCTT CACCTGTAGT AACTGCAAGC AAGTCATCGG GACTGGAAGC
TTCTTCCCTA AAGGGGAGGA CTTCTACTGC GTGACTTGCC ATGAGACCAA GTTTGCCAAG
CATTGCGTGA AGTGCAACAA GGGTTTGGTA AAGGCTCCAG TGTGGTGGCC TATGAAGGAC
AATCCTGGCA CGACTACTGC TTCCACTGCA AAAAATGCTC CGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001153175.1
CDS330..914
Misc Feature(1)300..302(+)
Misc Feature(2)342..428(+)
Misc Feature(3)348..422(+)
Misc Feature(4)447..608(+)
Misc Feature(5)447..605(+)
Misc Feature(6)630..803(+)
Misc Feature(7)630..788(+)
Exon (1)1..229
Gene:FHL1
Gene Synonym:
Exon (2)230..303
Gene:FHL1
Gene Synonym:
Exon (3)304..485
Gene:FHL1
Gene Synonym:
Exon (4)486..660
Gene:FHL1
Gene Synonym:
Exon (5)661..830
Gene:FHL1
Gene Synonym:
Exon (6)831..2319
Gene:FHL1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001159703
Organism Homo sapiens (human)
Definition Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001159703

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGCGGAGA AGTTTGACTG CCACTACTGC AGGGATCCCT TGCAGGGGAA GAAGTATGTG 
CAAAAGGATG GCCACCACTG CTGCCTGAAA TGCTTTGACA AGTTCTGTGC CAACACCTGT
GTGGAATGCC GCAAGCCCAT CGGTGCGGAC TCCAAGGAGG TGCACTATAA GAACCGCTTC
TGGCATGACA CCTGCTTCCG CTGTGCCAAG TGCCTTCACC CCTTGGCCAA TGAGACCTTT
GTGGCCAAGG ACAACAAGAT CCTGTGCAAC AAGTGCACCA CTCGGGAGGA CTCCCCCAAG
TGCAAGGGGT GCTTCAAGGC CATTGTGGCA GGAGATCAAA ACGTGGAGTA CAAGGGGACC
GTCTGGCACA AAGACTGCTT CACCTGTAGT AACTGCAAGC AAGTCATCGG GACTGGAAGC
TTCTTCCCTA AAGGGGAGGA CTTCTACTGC GTGACTTGCC ATGAGACCAA GTTTGCCAAG
CATTGCGTGA AGTGCAACAA GGGTTTGGTA AAGGCTCCAG TGTGGTGGCC TATGAAGGAC
AATCCTGGCA CGACTACTGC TTCCACTGCA AAAAATGCTC CGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16400
Clone ID Related Accession (Same CDS sequence) NM_001159700 , NM_001159704 , NM_001167819 , NM_001449 , XM_006724747
Accession Version NM_001159704.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 843bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product four and a half LIM domains protein 1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK122708.1, AL078638.9 and BM995585.1. Transcript Variant: This variant (4) differs in the 5' UTR, 3' UTR, and 3' coding region compared to variant 1. The encoded protein (isoform 2) has a distinct C-terminus and is shorter than isoform 1. Variants 2, 3, 4 and 9 encode the same protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK122708.1, U60115.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCGGAGA AGTTTGACTG CCACTACTGC AGGGATCCCT TGCAGGGGAA GAAGTATGTG 
CAAAAGGATG GCCACCACTG CTGCCTGAAA TGCTTTGACA AGTTCTGTGC CAACACCTGT
GTGGAATGCC GCAAGCCCAT CGGTGCGGAC TCCAAGGAGG TGCACTATAA GAACCGCTTC
TGGCATGACA CCTGCTTCCG CTGTGCCAAG TGCCTTCACC CCTTGGCCAA TGAGACCTTT
GTGGCCAAGG ACAACAAGAT CCTGTGCAAC AAGTGCACCA CTCGGGAGGA CTCCCCCAAG
TGCAAGGGGT GCTTCAAGGC CATTGTGGCA GGAGATCAAA ACGTGGAGTA CAAGGGGACC
GTCTGGCACA AAGACTGCTT CACCTGTAGT AACTGCAAGC AAGTCATCGG GACTGGAAGC
TTCTTCCCTA AAGGGGAGGA CTTCTACTGC GTGACTTGCC ATGAGACCAA GTTTGCCAAG
CATTGCGTGA AGTGCAACAA GGCCATCACA TCTGGAGGAA TCACTTACCA GGATCAGCCC
TGGCATGCCG ATTGCTTTGT GTGTGTTACC TGCTCTAAGA AGCTGGCTGG GCAGCGTTTC
ACCGCTGTGG AGGACCAGTA TTACTGCGTG GATTGCTACA AGAACTTTGT GGCCAAGAAG
TGTGCTGGAT GCAAGAACCC CATCACTGGG TTTGGTAAAG GCTCCAGTGT GGTGGCCTAT
GAAGGACAAT CCTGGCACGA CTACTGCTTC CACTGCAAAA AATGCTCCGT GAATCTGGCC
AACAAGCGCT TTGTTTTCCA CCAGGAGCAA GTGTATTGTC CCGACTGTGC CAAAAAGCTG
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001153176.1
CDS712..1554
Misc Feature(1)682..684(+)
Misc Feature(2)724..810(+)
Misc Feature(3)730..804(+)
Misc Feature(4)829..990(+)
Misc Feature(5)829..987(+)
Misc Feature(6)1012..1185(+)
Misc Feature(7)1012..1170(+)
Misc Feature(8)1195..1353(+)
Misc Feature(9)1195..1347(+)
Misc Feature(10)1360..1551(+)
Misc Feature(11)1372..1539(+)
Exon (1)1..685
Gene:FHL1
Gene Synonym:
Exon (2)686..867
Gene:FHL1
Gene Synonym:
Exon (3)868..1042
Gene:FHL1
Gene Synonym:
Exon (4)1043..1212
Gene:FHL1
Gene Synonym:
Exon (5)1213..1399
Gene:FHL1
Gene Synonym:
Exon (6)1400..2888
Gene:FHL1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001159704
Organism Homo sapiens (human)
Definition Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001159704

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCGGAGA AGTTTGACTG CCACTACTGC AGGGATCCCT TGCAGGGGAA GAAGTATGTG 
CAAAAGGATG GCCACCACTG CTGCCTGAAA TGCTTTGACA AGTTCTGTGC CAACACCTGT
GTGGAATGCC GCAAGCCCAT CGGTGCGGAC TCCAAGGAGG TGCACTATAA GAACCGCTTC
TGGCATGACA CCTGCTTCCG CTGTGCCAAG TGCCTTCACC CCTTGGCCAA TGAGACCTTT
GTGGCCAAGG ACAACAAGAT CCTGTGCAAC AAGTGCACCA CTCGGGAGGA CTCCCCCAAG
TGCAAGGGGT GCTTCAAGGC CATTGTGGCA GGAGATCAAA ACGTGGAGTA CAAGGGGACC
GTCTGGCACA AAGACTGCTT CACCTGTAGT AACTGCAAGC AAGTCATCGG GACTGGAAGC
TTCTTCCCTA AAGGGGAGGA CTTCTACTGC GTGACTTGCC ATGAGACCAA GTTTGCCAAG
CATTGCGTGA AGTGCAACAA GGCCATCACA TCTGGAGGAA TCACTTACCA GGATCAGCCC
TGGCATGCCG ATTGCTTTGT GTGTGTTACC TGCTCTAAGA AGCTGGCTGG GCAGCGTTTC
ACCGCTGTGG AGGACCAGTA TTACTGCGTG GATTGCTACA AGAACTTTGT GGCCAAGAAG
TGTGCTGGAT GCAAGAACCC CATCACTGGG TTTGGTAAAG GCTCCAGTGT GGTGGCCTAT
GAAGGACAAT CCTGGCACGA CTACTGCTTC CACTGCAAAA AATGCTCCGT GAATCTGGCC
AACAAGCGCT TTGTTTTCCA CCAGGAGCAA GTGTATTGTC CCGACTGTGC CAAAAAGCTG
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16400
Clone ID Related Accession (Same CDS sequence) NM_001159700 , NM_001159704 , NM_001167819 , NM_001449 , XM_006724747
Accession Version NM_001167819.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 843bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product four and a half LIM domains protein 1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA982372.1, DA917361.1, BC010998.2 and BM995585.1. Transcript Variant: This variant (9) uses a different segment for its 5' UTR and lacks an alternate exon in the 3' coding region, compared to variant 1. The encoded protein (isoform 2) has a distinct C-terminus and is shorter than isoform 1. Variants 2, 3, 4 and 9 encode the same protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC010998.2, AK223299.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCGGAGA AGTTTGACTG CCACTACTGC AGGGATCCCT TGCAGGGGAA GAAGTATGTG 
CAAAAGGATG GCCACCACTG CTGCCTGAAA TGCTTTGACA AGTTCTGTGC CAACACCTGT
GTGGAATGCC GCAAGCCCAT CGGTGCGGAC TCCAAGGAGG TGCACTATAA GAACCGCTTC
TGGCATGACA CCTGCTTCCG CTGTGCCAAG TGCCTTCACC CCTTGGCCAA TGAGACCTTT
GTGGCCAAGG ACAACAAGAT CCTGTGCAAC AAGTGCACCA CTCGGGAGGA CTCCCCCAAG
TGCAAGGGGT GCTTCAAGGC CATTGTGGCA GGAGATCAAA ACGTGGAGTA CAAGGGGACC
GTCTGGCACA AAGACTGCTT CACCTGTAGT AACTGCAAGC AAGTCATCGG GACTGGAAGC
TTCTTCCCTA AAGGGGAGGA CTTCTACTGC GTGACTTGCC ATGAGACCAA GTTTGCCAAG
CATTGCGTGA AGTGCAACAA GGCCATCACA TCTGGAGGAA TCACTTACCA GGATCAGCCC
TGGCATGCCG ATTGCTTTGT GTGTGTTACC TGCTCTAAGA AGCTGGCTGG GCAGCGTTTC
ACCGCTGTGG AGGACCAGTA TTACTGCGTG GATTGCTACA AGAACTTTGT GGCCAAGAAG
TGTGCTGGAT GCAAGAACCC CATCACTGGG TTTGGTAAAG GCTCCAGTGT GGTGGCCTAT
GAAGGACAAT CCTGGCACGA CTACTGCTTC CACTGCAAAA AATGCTCCGT GAATCTGGCC
AACAAGCGCT TTGTTTTCCA CCAGGAGCAA GTGTATTGTC CCGACTGTGC CAAAAAGCTG
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001161291.1
CDS198..1040
Misc Feature(1)168..170(+)
Misc Feature(2)210..296(+)
Misc Feature(3)216..290(+)
Misc Feature(4)315..476(+)
Misc Feature(5)315..473(+)
Misc Feature(6)498..671(+)
Misc Feature(7)498..656(+)
Misc Feature(8)681..839(+)
Misc Feature(9)681..833(+)
Misc Feature(10)846..1037(+)
Misc Feature(11)858..1025(+)
Exon (1)1..97
Gene:FHL1
Gene Synonym:
Exon (2)98..171
Gene:FHL1
Gene Synonym:
Exon (3)172..353
Gene:FHL1
Gene Synonym:
Exon (4)354..528
Gene:FHL1
Gene Synonym:
Exon (5)529..698
Gene:FHL1
Gene Synonym:
Exon (6)699..885
Gene:FHL1
Gene Synonym:
Exon (7)886..2374
Gene:FHL1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001167819
Organism Homo sapiens (human)
Definition Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant 9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001167819

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCGGAGA AGTTTGACTG CCACTACTGC AGGGATCCCT TGCAGGGGAA GAAGTATGTG 
CAAAAGGATG GCCACCACTG CTGCCTGAAA TGCTTTGACA AGTTCTGTGC CAACACCTGT
GTGGAATGCC GCAAGCCCAT CGGTGCGGAC TCCAAGGAGG TGCACTATAA GAACCGCTTC
TGGCATGACA CCTGCTTCCG CTGTGCCAAG TGCCTTCACC CCTTGGCCAA TGAGACCTTT
GTGGCCAAGG ACAACAAGAT CCTGTGCAAC AAGTGCACCA CTCGGGAGGA CTCCCCCAAG
TGCAAGGGGT GCTTCAAGGC CATTGTGGCA GGAGATCAAA ACGTGGAGTA CAAGGGGACC
GTCTGGCACA AAGACTGCTT CACCTGTAGT AACTGCAAGC AAGTCATCGG GACTGGAAGC
TTCTTCCCTA AAGGGGAGGA CTTCTACTGC GTGACTTGCC ATGAGACCAA GTTTGCCAAG
CATTGCGTGA AGTGCAACAA GGCCATCACA TCTGGAGGAA TCACTTACCA GGATCAGCCC
TGGCATGCCG ATTGCTTTGT GTGTGTTACC TGCTCTAAGA AGCTGGCTGG GCAGCGTTTC
ACCGCTGTGG AGGACCAGTA TTACTGCGTG GATTGCTACA AGAACTTTGT GGCCAAGAAG
TGTGCTGGAT GCAAGAACCC CATCACTGGG TTTGGTAAAG GCTCCAGTGT GGTGGCCTAT
GAAGGACAAT CCTGGCACGA CTACTGCTTC CACTGCAAAA AATGCTCCGT GAATCTGGCC
AACAAGCGCT TTGTTTTCCA CCAGGAGCAA GTGTATTGTC CCGACTGTGC CAAAAAGCTG
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16400
Clone ID Related Accession (Same CDS sequence) NM_001159700 , NM_001159704 , NM_001167819 , NM_001449 , XM_006724747
Accession Version NM_001449.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 843bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product four and a half LIM domains protein 1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA497183.1, BC010998.2 and BM995585.1. This sequence is a reference standard in the RefSeqGene project. On Apr 30, 2009 this sequence version replaced gi:34147646. Transcript Variant: This variant (2) differs in the 5' UTR, 3' UTR, and 3' coding region compared to variant 1. The encoded protein (isoform 2) has a distinct C-terminus and is shorter than isoform 1. Variants 2, 3, 4 and 9 encode the same protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC010998.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCGGAGA AGTTTGACTG CCACTACTGC AGGGATCCCT TGCAGGGGAA GAAGTATGTG 
CAAAAGGATG GCCACCACTG CTGCCTGAAA TGCTTTGACA AGTTCTGTGC CAACACCTGT
GTGGAATGCC GCAAGCCCAT CGGTGCGGAC TCCAAGGAGG TGCACTATAA GAACCGCTTC
TGGCATGACA CCTGCTTCCG CTGTGCCAAG TGCCTTCACC CCTTGGCCAA TGAGACCTTT
GTGGCCAAGG ACAACAAGAT CCTGTGCAAC AAGTGCACCA CTCGGGAGGA CTCCCCCAAG
TGCAAGGGGT GCTTCAAGGC CATTGTGGCA GGAGATCAAA ACGTGGAGTA CAAGGGGACC
GTCTGGCACA AAGACTGCTT CACCTGTAGT AACTGCAAGC AAGTCATCGG GACTGGAAGC
TTCTTCCCTA AAGGGGAGGA CTTCTACTGC GTGACTTGCC ATGAGACCAA GTTTGCCAAG
CATTGCGTGA AGTGCAACAA GGCCATCACA TCTGGAGGAA TCACTTACCA GGATCAGCCC
TGGCATGCCG ATTGCTTTGT GTGTGTTACC TGCTCTAAGA AGCTGGCTGG GCAGCGTTTC
ACCGCTGTGG AGGACCAGTA TTACTGCGTG GATTGCTACA AGAACTTTGT GGCCAAGAAG
TGTGCTGGAT GCAAGAACCC CATCACTGGG TTTGGTAAAG GCTCCAGTGT GGTGGCCTAT
GAAGGACAAT CCTGGCACGA CTACTGCTTC CACTGCAAAA AATGCTCCGT GAATCTGGCC
AACAAGCGCT TTGTTTTCCA CCAGGAGCAA GTGTATTGTC CCGACTGTGC CAAAAAGCTG
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001440.2
CDS330..1172
Misc Feature(1)300..302(+)
Misc Feature(2)342..428(+)
Misc Feature(3)348..422(+)
Misc Feature(4)447..608(+)
Misc Feature(5)447..605(+)
Misc Feature(6)630..803(+)
Misc Feature(7)630..788(+)
Misc Feature(8)813..971(+)
Misc Feature(9)813..965(+)
Misc Feature(10)978..1169(+)
Misc Feature(11)990..1157(+)
Exon (1)1..229
Gene:FHL1
Gene Synonym:
Exon (2)230..303
Gene:FHL1
Gene Synonym:
Exon (3)304..485
Gene:FHL1
Gene Synonym:
Exon (4)486..660
Gene:FHL1
Gene Synonym:
Exon (5)661..830
Gene:FHL1
Gene Synonym:
Exon (6)831..1017
Gene:FHL1
Gene Synonym:
Exon (7)1018..2506
Gene:FHL1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001449
Organism Homo sapiens (human)
Definition Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001449

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCGGAGA AGTTTGACTG CCACTACTGC AGGGATCCCT TGCAGGGGAA GAAGTATGTG 
CAAAAGGATG GCCACCACTG CTGCCTGAAA TGCTTTGACA AGTTCTGTGC CAACACCTGT
GTGGAATGCC GCAAGCCCAT CGGTGCGGAC TCCAAGGAGG TGCACTATAA GAACCGCTTC
TGGCATGACA CCTGCTTCCG CTGTGCCAAG TGCCTTCACC CCTTGGCCAA TGAGACCTTT
GTGGCCAAGG ACAACAAGAT CCTGTGCAAC AAGTGCACCA CTCGGGAGGA CTCCCCCAAG
TGCAAGGGGT GCTTCAAGGC CATTGTGGCA GGAGATCAAA ACGTGGAGTA CAAGGGGACC
GTCTGGCACA AAGACTGCTT CACCTGTAGT AACTGCAAGC AAGTCATCGG GACTGGAAGC
TTCTTCCCTA AAGGGGAGGA CTTCTACTGC GTGACTTGCC ATGAGACCAA GTTTGCCAAG
CATTGCGTGA AGTGCAACAA GGCCATCACA TCTGGAGGAA TCACTTACCA GGATCAGCCC
TGGCATGCCG ATTGCTTTGT GTGTGTTACC TGCTCTAAGA AGCTGGCTGG GCAGCGTTTC
ACCGCTGTGG AGGACCAGTA TTACTGCGTG GATTGCTACA AGAACTTTGT GGCCAAGAAG
TGTGCTGGAT GCAAGAACCC CATCACTGGG TTTGGTAAAG GCTCCAGTGT GGTGGCCTAT
GAAGGACAAT CCTGGCACGA CTACTGCTTC CACTGCAAAA AATGCTCCGT GAATCTGGCC
AACAAGCGCT TTGTTTTCCA CCAGGAGCAA GTGTATTGTC CCGACTGTGC CAAAAAGCTG
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu54313
Clone ID Related Accession (Same CDS sequence) XM_006724743
Accession Version XM_006724743.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1020bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product four and a half LIM domains protein 1 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011786.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578838644. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTTCCC ATAGACACTC AGGTCCCTCC AGCTACAAGG TGGGCACCAT GGCGGAGAAG 
TTTGACTGCC ACTACTGCAG GGATCCCTTG CAGGGGAAGA AGTATGTGCA AAAGGATGGC
CACCACTGCT GCCTGAAATG CTTTGACAAG TTCTGTGCCA ACACCTGTGT GGAATGCCGC
AAGCCCATCG GTGCGGACTC CAAGGAGGTG CACTATAAGA ACCGCTTCTG GCATGACACC
TGCTTCCGCT GTGCCAAGTG CCTTCACCCC TTGGCCAATG AGACCTTTGT GGCCAAGGAC
AACAAGATCC TGTGCAACAA GTGCACCACT CGGGAGGACT CCCCCAAGTG CAAGGGGTGC
TTCAAGGCCA TTGTGGCAGG AGATCAAAAC GTGGAGTACA AGGGGACCGT CTGGCACAAA
GACTGCTTCA CCTGTAGTAA CTGCAAGCAA GTCATCGGGA CTGGAAGCTT CTTCCCTAAA
GGGGAGGACT TCTACTGCGT GACTTGCCAT GAGACCAAGT TTGCCAAGCA TTGCGTGAAG
TGCAACAAGG CCATCACATC TGGAGGAATC ACTTACCAGG ATCAGCCCTG GCATGCCGAT
TGCTTTGTGT GTGTTACCTG CTCTAAGAAG CTGGCTGGGC AGCGTTTCAC CGCTGTGGAG
GACCAGTATT ACTGCGTGGA TTGCTACAAG AACTTTGTGG CCAAGAAGTG TGCTGGATGC
AAGAACCCCA TCACTGGGAA AAGGACTGTG TCAAGAGTGA GCCACCCAGT CTCTAAAGCT
AGGAAGCCCC CAGTGTGCCA CGGGAAACGC TTGCCTCTCA CCCTGTTTCC CAGCGCCAAC
CTCCGGGGCA GGCATCCGGG TGGAGAGAGG ACTTGTCCCT CGTGGGTGGT GGTTCTTTAT
AGAAAAAATC GAAGCTTAGC AGCTCCTCGA GGCCCGGGTT TGGTAAAGGC TCCAGTGTGG
TGGCCTATGA AGGACAATCC TGGCACGACT ACTGCTTCCA CTGCAAAAAA TGCTCCGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006724806.1
CDS86..1105
Misc Feature(1)146..232(+)
Misc Feature(2)152..226(+)
Misc Feature(3)251..412(+)
Misc Feature(4)251..409(+)
Misc Feature(5)434..607(+)
Misc Feature(6)434..592(+)
Misc Feature(7)617..775(+)
Misc Feature(8)617..769(+)
Translation

Target ORF information:

RefSeq Version XM_006724743
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006724743

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTTCCC ATAGACACTC AGGTCCCTCC AGCTACAAGG TGGGCACCAT GGCGGAGAAG 
TTTGACTGCC ACTACTGCAG GGATCCCTTG CAGGGGAAGA AGTATGTGCA AAAGGATGGC
CACCACTGCT GCCTGAAATG CTTTGACAAG TTCTGTGCCA ACACCTGTGT GGAATGCCGC
AAGCCCATCG GTGCGGACTC CAAGGAGGTG CACTATAAGA ACCGCTTCTG GCATGACACC
TGCTTCCGCT GTGCCAAGTG CCTTCACCCC TTGGCCAATG AGACCTTTGT GGCCAAGGAC
AACAAGATCC TGTGCAACAA GTGCACCACT CGGGAGGACT CCCCCAAGTG CAAGGGGTGC
TTCAAGGCCA TTGTGGCAGG AGATCAAAAC GTGGAGTACA AGGGGACCGT CTGGCACAAA
GACTGCTTCA CCTGTAGTAA CTGCAAGCAA GTCATCGGGA CTGGAAGCTT CTTCCCTAAA
GGGGAGGACT TCTACTGCGT GACTTGCCAT GAGACCAAGT TTGCCAAGCA TTGCGTGAAG
TGCAACAAGG CCATCACATC TGGAGGAATC ACTTACCAGG ATCAGCCCTG GCATGCCGAT
TGCTTTGTGT GTGTTACCTG CTCTAAGAAG CTGGCTGGGC AGCGTTTCAC CGCTGTGGAG
GACCAGTATT ACTGCGTGGA TTGCTACAAG AACTTTGTGG CCAAGAAGTG TGCTGGATGC
AAGAACCCCA TCACTGGGAA AAGGACTGTG TCAAGAGTGA GCCACCCAGT CTCTAAAGCT
AGGAAGCCCC CAGTGTGCCA CGGGAAACGC TTGCCTCTCA CCCTGTTTCC CAGCGCCAAC
CTCCGGGGCA GGCATCCGGG TGGAGAGAGG ACTTGTCCCT CGTGGGTGGT GGTTCTTTAT
AGAAAAAATC GAAGCTTAGC AGCTCCTCGA GGCCCGGGTT TGGTAAAGGC TCCAGTGTGG
TGGCCTATGA AGGACAATCC TGGCACGACT ACTGCTTCCA CTGCAAAAAA TGCTCCGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19535
Clone ID Related Accession (Same CDS sequence) NM_001159702 , XM_006724744 , XM_006724745 , XM_006724746
Accession Version XM_006724744.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 972bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product four and a half LIM domains protein 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011786.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578838646. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCGGAGA AGTTTGACTG CCACTACTGC AGGGATCCCT TGCAGGGGAA GAAGTATGTG 
CAAAAGGATG GCCACCACTG CTGCCTGAAA TGCTTTGACA AGTTCTGTGC CAACACCTGT
GTGGAATGCC GCAAGCCCAT CGGTGCGGAC TCCAAGGAGG TGCACTATAA GAACCGCTTC
TGGCATGACA CCTGCTTCCG CTGTGCCAAG TGCCTTCACC CCTTGGCCAA TGAGACCTTT
GTGGCCAAGG ACAACAAGAT CCTGTGCAAC AAGTGCACCA CTCGGGAGGA CTCCCCCAAG
TGCAAGGGGT GCTTCAAGGC CATTGTGGCA GGAGATCAAA ACGTGGAGTA CAAGGGGACC
GTCTGGCACA AAGACTGCTT CACCTGTAGT AACTGCAAGC AAGTCATCGG GACTGGAAGC
TTCTTCCCTA AAGGGGAGGA CTTCTACTGC GTGACTTGCC ATGAGACCAA GTTTGCCAAG
CATTGCGTGA AGTGCAACAA GGCCATCACA TCTGGAGGAA TCACTTACCA GGATCAGCCC
TGGCATGCCG ATTGCTTTGT GTGTGTTACC TGCTCTAAGA AGCTGGCTGG GCAGCGTTTC
ACCGCTGTGG AGGACCAGTA TTACTGCGTG GATTGCTACA AGAACTTTGT GGCCAAGAAG
TGTGCTGGAT GCAAGAACCC CATCACTGGG AAAAGGACTG TGTCAAGAGT GAGCCACCCA
GTCTCTAAAG CTAGGAAGCC CCCAGTGTGC CACGGGAAAC GCTTGCCTCT CACCCTGTTT
CCCAGCGCCA ACCTCCGGGG CAGGCATCCG GGTGGAGAGA GGACTTGTCC CTCGTGGGTG
GTGGTTCTTT ATAGAAAAAA TCGAAGCTTA GCAGCTCCTC GAGGCCCGGG TTTGGTAAAG
GCTCCAGTGT GGTGGCCTAT GAAGGACAAT CCTGGCACGA CTACTGCTTC CACTGCAAAA
AATGCTCCGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006724807.1
CDS212..1183
Misc Feature(1)224..310(+)
Misc Feature(2)230..304(+)
Misc Feature(3)329..490(+)
Misc Feature(4)329..487(+)
Misc Feature(5)512..685(+)
Misc Feature(6)512..670(+)
Misc Feature(7)695..853(+)
Misc Feature(8)695..847(+)
Translation

Target ORF information:

RefSeq Version XM_006724744
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006724744

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCGGAGA AGTTTGACTG CCACTACTGC AGGGATCCCT TGCAGGGGAA GAAGTATGTG 
CAAAAGGATG GCCACCACTG CTGCCTGAAA TGCTTTGACA AGTTCTGTGC CAACACCTGT
GTGGAATGCC GCAAGCCCAT CGGTGCGGAC TCCAAGGAGG TGCACTATAA GAACCGCTTC
TGGCATGACA CCTGCTTCCG CTGTGCCAAG TGCCTTCACC CCTTGGCCAA TGAGACCTTT
GTGGCCAAGG ACAACAAGAT CCTGTGCAAC AAGTGCACCA CTCGGGAGGA CTCCCCCAAG
TGCAAGGGGT GCTTCAAGGC CATTGTGGCA GGAGATCAAA ACGTGGAGTA CAAGGGGACC
GTCTGGCACA AAGACTGCTT CACCTGTAGT AACTGCAAGC AAGTCATCGG GACTGGAAGC
TTCTTCCCTA AAGGGGAGGA CTTCTACTGC GTGACTTGCC ATGAGACCAA GTTTGCCAAG
CATTGCGTGA AGTGCAACAA GGCCATCACA TCTGGAGGAA TCACTTACCA GGATCAGCCC
TGGCATGCCG ATTGCTTTGT GTGTGTTACC TGCTCTAAGA AGCTGGCTGG GCAGCGTTTC
ACCGCTGTGG AGGACCAGTA TTACTGCGTG GATTGCTACA AGAACTTTGT GGCCAAGAAG
TGTGCTGGAT GCAAGAACCC CATCACTGGG AAAAGGACTG TGTCAAGAGT GAGCCACCCA
GTCTCTAAAG CTAGGAAGCC CCCAGTGTGC CACGGGAAAC GCTTGCCTCT CACCCTGTTT
CCCAGCGCCA ACCTCCGGGG CAGGCATCCG GGTGGAGAGA GGACTTGTCC CTCGTGGGTG
GTGGTTCTTT ATAGAAAAAA TCGAAGCTTA GCAGCTCCTC GAGGCCCGGG TTTGGTAAAG
GCTCCAGTGT GGTGGCCTAT GAAGGACAAT CCTGGCACGA CTACTGCTTC CACTGCAAAA
AATGCTCCGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19535
Clone ID Related Accession (Same CDS sequence) NM_001159702 , XM_006724744 , XM_006724745 , XM_006724746
Accession Version XM_006724745.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 972bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product four and a half LIM domains protein 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011786.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578838648. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCGGAGA AGTTTGACTG CCACTACTGC AGGGATCCCT TGCAGGGGAA GAAGTATGTG 
CAAAAGGATG GCCACCACTG CTGCCTGAAA TGCTTTGACA AGTTCTGTGC CAACACCTGT
GTGGAATGCC GCAAGCCCAT CGGTGCGGAC TCCAAGGAGG TGCACTATAA GAACCGCTTC
TGGCATGACA CCTGCTTCCG CTGTGCCAAG TGCCTTCACC CCTTGGCCAA TGAGACCTTT
GTGGCCAAGG ACAACAAGAT CCTGTGCAAC AAGTGCACCA CTCGGGAGGA CTCCCCCAAG
TGCAAGGGGT GCTTCAAGGC CATTGTGGCA GGAGATCAAA ACGTGGAGTA CAAGGGGACC
GTCTGGCACA AAGACTGCTT CACCTGTAGT AACTGCAAGC AAGTCATCGG GACTGGAAGC
TTCTTCCCTA AAGGGGAGGA CTTCTACTGC GTGACTTGCC ATGAGACCAA GTTTGCCAAG
CATTGCGTGA AGTGCAACAA GGCCATCACA TCTGGAGGAA TCACTTACCA GGATCAGCCC
TGGCATGCCG ATTGCTTTGT GTGTGTTACC TGCTCTAAGA AGCTGGCTGG GCAGCGTTTC
ACCGCTGTGG AGGACCAGTA TTACTGCGTG GATTGCTACA AGAACTTTGT GGCCAAGAAG
TGTGCTGGAT GCAAGAACCC CATCACTGGG AAAAGGACTG TGTCAAGAGT GAGCCACCCA
GTCTCTAAAG CTAGGAAGCC CCCAGTGTGC CACGGGAAAC GCTTGCCTCT CACCCTGTTT
CCCAGCGCCA ACCTCCGGGG CAGGCATCCG GGTGGAGAGA GGACTTGTCC CTCGTGGGTG
GTGGTTCTTT ATAGAAAAAA TCGAAGCTTA GCAGCTCCTC GAGGCCCGGG TTTGGTAAAG
GCTCCAGTGT GGTGGCCTAT GAAGGACAAT CCTGGCACGA CTACTGCTTC CACTGCAAAA
AATGCTCCGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006724808.1
CDS167..1138
Misc Feature(1)179..265(+)
Misc Feature(2)185..259(+)
Misc Feature(3)284..445(+)
Misc Feature(4)284..442(+)
Misc Feature(5)467..640(+)
Misc Feature(6)467..625(+)
Misc Feature(7)650..808(+)
Misc Feature(8)650..802(+)
Translation

Target ORF information:

RefSeq Version XM_006724745
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006724745

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCGGAGA AGTTTGACTG CCACTACTGC AGGGATCCCT TGCAGGGGAA GAAGTATGTG 
CAAAAGGATG GCCACCACTG CTGCCTGAAA TGCTTTGACA AGTTCTGTGC CAACACCTGT
GTGGAATGCC GCAAGCCCAT CGGTGCGGAC TCCAAGGAGG TGCACTATAA GAACCGCTTC
TGGCATGACA CCTGCTTCCG CTGTGCCAAG TGCCTTCACC CCTTGGCCAA TGAGACCTTT
GTGGCCAAGG ACAACAAGAT CCTGTGCAAC AAGTGCACCA CTCGGGAGGA CTCCCCCAAG
TGCAAGGGGT GCTTCAAGGC CATTGTGGCA GGAGATCAAA ACGTGGAGTA CAAGGGGACC
GTCTGGCACA AAGACTGCTT CACCTGTAGT AACTGCAAGC AAGTCATCGG GACTGGAAGC
TTCTTCCCTA AAGGGGAGGA CTTCTACTGC GTGACTTGCC ATGAGACCAA GTTTGCCAAG
CATTGCGTGA AGTGCAACAA GGCCATCACA TCTGGAGGAA TCACTTACCA GGATCAGCCC
TGGCATGCCG ATTGCTTTGT GTGTGTTACC TGCTCTAAGA AGCTGGCTGG GCAGCGTTTC
ACCGCTGTGG AGGACCAGTA TTACTGCGTG GATTGCTACA AGAACTTTGT GGCCAAGAAG
TGTGCTGGAT GCAAGAACCC CATCACTGGG AAAAGGACTG TGTCAAGAGT GAGCCACCCA
GTCTCTAAAG CTAGGAAGCC CCCAGTGTGC CACGGGAAAC GCTTGCCTCT CACCCTGTTT
CCCAGCGCCA ACCTCCGGGG CAGGCATCCG GGTGGAGAGA GGACTTGTCC CTCGTGGGTG
GTGGTTCTTT ATAGAAAAAA TCGAAGCTTA GCAGCTCCTC GAGGCCCGGG TTTGGTAAAG
GCTCCAGTGT GGTGGCCTAT GAAGGACAAT CCTGGCACGA CTACTGCTTC CACTGCAAAA
AATGCTCCGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19535
Clone ID Related Accession (Same CDS sequence) NM_001159702 , XM_006724744 , XM_006724745 , XM_006724746
Accession Version XM_006724746.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 972bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product four and a half LIM domains protein 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011786.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578838650. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCGGAGA AGTTTGACTG CCACTACTGC AGGGATCCCT TGCAGGGGAA GAAGTATGTG 
CAAAAGGATG GCCACCACTG CTGCCTGAAA TGCTTTGACA AGTTCTGTGC CAACACCTGT
GTGGAATGCC GCAAGCCCAT CGGTGCGGAC TCCAAGGAGG TGCACTATAA GAACCGCTTC
TGGCATGACA CCTGCTTCCG CTGTGCCAAG TGCCTTCACC CCTTGGCCAA TGAGACCTTT
GTGGCCAAGG ACAACAAGAT CCTGTGCAAC AAGTGCACCA CTCGGGAGGA CTCCCCCAAG
TGCAAGGGGT GCTTCAAGGC CATTGTGGCA GGAGATCAAA ACGTGGAGTA CAAGGGGACC
GTCTGGCACA AAGACTGCTT CACCTGTAGT AACTGCAAGC AAGTCATCGG GACTGGAAGC
TTCTTCCCTA AAGGGGAGGA CTTCTACTGC GTGACTTGCC ATGAGACCAA GTTTGCCAAG
CATTGCGTGA AGTGCAACAA GGCCATCACA TCTGGAGGAA TCACTTACCA GGATCAGCCC
TGGCATGCCG ATTGCTTTGT GTGTGTTACC TGCTCTAAGA AGCTGGCTGG GCAGCGTTTC
ACCGCTGTGG AGGACCAGTA TTACTGCGTG GATTGCTACA AGAACTTTGT GGCCAAGAAG
TGTGCTGGAT GCAAGAACCC CATCACTGGG AAAAGGACTG TGTCAAGAGT GAGCCACCCA
GTCTCTAAAG CTAGGAAGCC CCCAGTGTGC CACGGGAAAC GCTTGCCTCT CACCCTGTTT
CCCAGCGCCA ACCTCCGGGG CAGGCATCCG GGTGGAGAGA GGACTTGTCC CTCGTGGGTG
GTGGTTCTTT ATAGAAAAAA TCGAAGCTTA GCAGCTCCTC GAGGCCCGGG TTTGGTAAAG
GCTCCAGTGT GGTGGCCTAT GAAGGACAAT CCTGGCACGA CTACTGCTTC CACTGCAAAA
AATGCTCCGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006724809.1
CDS85..1056
Misc Feature(1)97..183(+)
Misc Feature(2)103..177(+)
Misc Feature(3)202..363(+)
Misc Feature(4)202..360(+)
Misc Feature(5)385..558(+)
Misc Feature(6)385..543(+)
Misc Feature(7)568..726(+)
Misc Feature(8)568..720(+)
Translation

Target ORF information:

RefSeq Version XM_006724746
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006724746

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCGGAGA AGTTTGACTG CCACTACTGC AGGGATCCCT TGCAGGGGAA GAAGTATGTG 
CAAAAGGATG GCCACCACTG CTGCCTGAAA TGCTTTGACA AGTTCTGTGC CAACACCTGT
GTGGAATGCC GCAAGCCCAT CGGTGCGGAC TCCAAGGAGG TGCACTATAA GAACCGCTTC
TGGCATGACA CCTGCTTCCG CTGTGCCAAG TGCCTTCACC CCTTGGCCAA TGAGACCTTT
GTGGCCAAGG ACAACAAGAT CCTGTGCAAC AAGTGCACCA CTCGGGAGGA CTCCCCCAAG
TGCAAGGGGT GCTTCAAGGC CATTGTGGCA GGAGATCAAA ACGTGGAGTA CAAGGGGACC
GTCTGGCACA AAGACTGCTT CACCTGTAGT AACTGCAAGC AAGTCATCGG GACTGGAAGC
TTCTTCCCTA AAGGGGAGGA CTTCTACTGC GTGACTTGCC ATGAGACCAA GTTTGCCAAG
CATTGCGTGA AGTGCAACAA GGCCATCACA TCTGGAGGAA TCACTTACCA GGATCAGCCC
TGGCATGCCG ATTGCTTTGT GTGTGTTACC TGCTCTAAGA AGCTGGCTGG GCAGCGTTTC
ACCGCTGTGG AGGACCAGTA TTACTGCGTG GATTGCTACA AGAACTTTGT GGCCAAGAAG
TGTGCTGGAT GCAAGAACCC CATCACTGGG AAAAGGACTG TGTCAAGAGT GAGCCACCCA
GTCTCTAAAG CTAGGAAGCC CCCAGTGTGC CACGGGAAAC GCTTGCCTCT CACCCTGTTT
CCCAGCGCCA ACCTCCGGGG CAGGCATCCG GGTGGAGAGA GGACTTGTCC CTCGTGGGTG
GTGGTTCTTT ATAGAAAAAA TCGAAGCTTA GCAGCTCCTC GAGGCCCGGG TTTGGTAAAG
GCTCCAGTGT GGTGGCCTAT GAAGGACAAT CCTGGCACGA CTACTGCTTC CACTGCAAAA
AATGCTCCGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16400
Clone ID Related Accession (Same CDS sequence) NM_001159700 , NM_001159704 , NM_001167819 , NM_001449 , XM_006724747
Accession Version XM_006724747.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 843bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product four and a half LIM domains protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011786.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578838652. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCGGAGA AGTTTGACTG CCACTACTGC AGGGATCCCT TGCAGGGGAA GAAGTATGTG 
CAAAAGGATG GCCACCACTG CTGCCTGAAA TGCTTTGACA AGTTCTGTGC CAACACCTGT
GTGGAATGCC GCAAGCCCAT CGGTGCGGAC TCCAAGGAGG TGCACTATAA GAACCGCTTC
TGGCATGACA CCTGCTTCCG CTGTGCCAAG TGCCTTCACC CCTTGGCCAA TGAGACCTTT
GTGGCCAAGG ACAACAAGAT CCTGTGCAAC AAGTGCACCA CTCGGGAGGA CTCCCCCAAG
TGCAAGGGGT GCTTCAAGGC CATTGTGGCA GGAGATCAAA ACGTGGAGTA CAAGGGGACC
GTCTGGCACA AAGACTGCTT CACCTGTAGT AACTGCAAGC AAGTCATCGG GACTGGAAGC
TTCTTCCCTA AAGGGGAGGA CTTCTACTGC GTGACTTGCC ATGAGACCAA GTTTGCCAAG
CATTGCGTGA AGTGCAACAA GGCCATCACA TCTGGAGGAA TCACTTACCA GGATCAGCCC
TGGCATGCCG ATTGCTTTGT GTGTGTTACC TGCTCTAAGA AGCTGGCTGG GCAGCGTTTC
ACCGCTGTGG AGGACCAGTA TTACTGCGTG GATTGCTACA AGAACTTTGT GGCCAAGAAG
TGTGCTGGAT GCAAGAACCC CATCACTGGG TTTGGTAAAG GCTCCAGTGT GGTGGCCTAT
GAAGGACAAT CCTGGCACGA CTACTGCTTC CACTGCAAAA AATGCTCCGT GAATCTGGCC
AACAAGCGCT TTGTTTTCCA CCAGGAGCAA GTGTATTGTC CCGACTGTGC CAAAAAGCTG
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006724810.1
CDS85..927
Misc Feature(1)97..183(+)
Misc Feature(2)103..177(+)
Misc Feature(3)202..363(+)
Misc Feature(4)202..360(+)
Misc Feature(5)385..558(+)
Misc Feature(6)385..543(+)
Misc Feature(7)568..726(+)
Misc Feature(8)568..720(+)
Misc Feature(9)733..924(+)
Misc Feature(10)745..912(+)
Translation

Target ORF information:

RefSeq Version XM_006724747
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006724747

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCGGAGA AGTTTGACTG CCACTACTGC AGGGATCCCT TGCAGGGGAA GAAGTATGTG 
CAAAAGGATG GCCACCACTG CTGCCTGAAA TGCTTTGACA AGTTCTGTGC CAACACCTGT
GTGGAATGCC GCAAGCCCAT CGGTGCGGAC TCCAAGGAGG TGCACTATAA GAACCGCTTC
TGGCATGACA CCTGCTTCCG CTGTGCCAAG TGCCTTCACC CCTTGGCCAA TGAGACCTTT
GTGGCCAAGG ACAACAAGAT CCTGTGCAAC AAGTGCACCA CTCGGGAGGA CTCCCCCAAG
TGCAAGGGGT GCTTCAAGGC CATTGTGGCA GGAGATCAAA ACGTGGAGTA CAAGGGGACC
GTCTGGCACA AAGACTGCTT CACCTGTAGT AACTGCAAGC AAGTCATCGG GACTGGAAGC
TTCTTCCCTA AAGGGGAGGA CTTCTACTGC GTGACTTGCC ATGAGACCAA GTTTGCCAAG
CATTGCGTGA AGTGCAACAA GGCCATCACA TCTGGAGGAA TCACTTACCA GGATCAGCCC
TGGCATGCCG ATTGCTTTGT GTGTGTTACC TGCTCTAAGA AGCTGGCTGG GCAGCGTTTC
ACCGCTGTGG AGGACCAGTA TTACTGCGTG GATTGCTACA AGAACTTTGT GGCCAAGAAG
TGTGCTGGAT GCAAGAACCC CATCACTGGG TTTGGTAAAG GCTCCAGTGT GGTGGCCTAT
GAAGGACAAT CCTGGCACGA CTACTGCTTC CACTGCAAAA AATGCTCCGT GAATCTGGCC
AACAAGCGCT TTGTTTTCCA CCAGGAGCAA GTGTATTGTC CCGACTGTGC CAAAAAGCTG
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57783
Clone ID Related Accession (Same CDS sequence) XM_011531316
Accession Version XM_011531316.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 633bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product four and a half LIM domains protein 1 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011786.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
ATGGCTTCCC ATAGACACTC AGGTCCCTCC AGCTACAAGG TGGGCACCAT GGCGGAGAAG 
TTTGACTGCC ACTACTGCAG GGATCCCTTG CAGGGGAAGA AGTATGTGCA AAAGGATGGC
CACCACTGCT GCCTGAAATG CTTTGACAAG TTCTGTGCCA ACACCTGTGT GGAATGCCGC
AAGCCCATCG GTGCGGACTC CAAGGAGGTG CACTATAAGA ACCGCTTCTG GCATGACACC
TGCTTCCGCT GTGCCAAGTG CCTTCACCCC TTGGCCAATG AGACCTTTGT GGCCAAGGAC
AACAAGATCC TGTGCAACAA GTGCACCACT CGGGAGGACT CCCCCAAGTG CAAGGGGTGC
TTCAAGGCCA TTGTGGCAGG AGATCAAAAC GTGGAGTACA AGGGGACCGT CTGGCACAAA
GACTGCTTCA CCTGTAGTAA CTGCAAGCAA GTCATCGGGA CTGGAAGCTT CTTCCCTAAA
GGGGAGGACT TCTACTGCGT GACTTGCCAT GAGACCAAGT TTGCCAAGCA TTGCGTGAAG
TGCAACAAGG GTTTGGTAAA GGCTCCAGTG TGGTGGCCTA TGAAGGACAA TCCTGGCACG
ACTACTGCTT CCACTGCAAA AAATGCTCCG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011529618.1
CDS86..718
Misc Feature(1)146..232(+)
Misc Feature(2)152..226(+)
Misc Feature(3)251..412(+)
Misc Feature(4)251..409(+)
Misc Feature(5)434..607(+)
Misc Feature(6)434..592(+)
Translation

Target ORF information:

RefSeq Version XM_011531316
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011531316

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCTTCCC ATAGACACTC AGGTCCCTCC AGCTACAAGG TGGGCACCAT GGCGGAGAAG 
TTTGACTGCC ACTACTGCAG GGATCCCTTG CAGGGGAAGA AGTATGTGCA AAAGGATGGC
CACCACTGCT GCCTGAAATG CTTTGACAAG TTCTGTGCCA ACACCTGTGT GGAATGCCGC
AAGCCCATCG GTGCGGACTC CAAGGAGGTG CACTATAAGA ACCGCTTCTG GCATGACACC
TGCTTCCGCT GTGCCAAGTG CCTTCACCCC TTGGCCAATG AGACCTTTGT GGCCAAGGAC
AACAAGATCC TGTGCAACAA GTGCACCACT CGGGAGGACT CCCCCAAGTG CAAGGGGTGC
TTCAAGGCCA TTGTGGCAGG AGATCAAAAC GTGGAGTACA AGGGGACCGT CTGGCACAAA
GACTGCTTCA CCTGTAGTAA CTGCAAGCAA GTCATCGGGA CTGGAAGCTT CTTCCCTAAA
GGGGAGGACT TCTACTGCGT GACTTGCCAT GAGACCAAGT TTGCCAAGCA TTGCGTGAAG
TGCAACAAGG GTTTGGTAAA GGCTCCAGTG TGGTGGCCTA TGAAGGACAA TCCTGGCACG
ACTACTGCTT CCACTGCAAA AAATGCTCCG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Skeletal muscle biopsy analysis in reducing body myopathy and other FHL1-related disorders
J. Neuropathol. Exp. Neurol. 72 (9), 833-845 (2013)
Malfatti E, Olive M, Taratuto AL, Richard P, Brochier G, Bitoun M, Gueneau L, Laforet P, Stojkovic T, Maisonobe T, Monges S, Lubieniecki F, Vasquez G, Streichenberger N, Lacene E, Saccoliti M, Prudhon B, Alexianu M, Figarella-Branger D, Schessl J, Bonnemann C, Eymard B, Fardeau M, Bonne G and Romero NB.


book

Consequences of mutations within the C terminus of the FHL1 gene
Neurology 73 (7), 543-551 (2009)
Schoser B, Goebel HH, Janisch I, Quasthoff S, Rother J, Bergmann M, Muller-Felber W and Windpassinger C.


book

Human four-and-a-half LIM family members suppress tumor cell growth through a TGF-beta-like signaling pathway
J. Clin. Invest. 119 (2), 349-361 (2009)
Ding L, Wang Z, Yan J, Yang X, Liu A, Qiu W, Zhu J, Han J, Zhang H, Lin J, Cheng L, Qin X, Niu C, Yuan B, Wang X, Zhu C, Zhou Y, Li J, Song H, Huang C and Ye Q.


book

Genomic structure, tissue expression and chromosomal location of the LIM-only gene, SLIM1
Gene 232 (2), 203-207 (1999)
Greene WK, Baker E, Rabbitts TH and Kees UR.


book

The LIM proteins FHL1 and FHL3 are expressed differently in skeletal muscle
Biochem. Biophys. Res. Commun. 255 (2), 245-250 (1999)
Morgan MJ and Madgwick AJ.


book

Chromosomal mapping, tissue distribution and cDNA sequence of four-and-a-half LIM domain protein 1 (FHL1)
Gene 216 (1), 163-170 (1998)
Lee SM, Tsui SK, Chan KK, Garcia-Barcelo M, Waye MM, Fung KP, Liew CC and Lee CY.


book

The developmental regulation of a novel muscle LIM-protein
Biochem. Biophys. Res. Commun. 212 (3), 840-846 (1995)
Morgan MJ, Madgwick AJ, Charleston B, Pell JM and Loughna PT.


book

Myofibrillar Myopathy
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Selcen,D. and Engel,A.G.


book

Hemophagocytic Lymphohistiocytosis, Familial
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Zhang,K., Filipovich,A.H., Johnson,J., Marsh,R.A. and Villanueva,J.


book

Emery-Dreifuss Muscular Dystrophy
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Bonne,G., Leturcq,F. and Ben Yaou,R.


 
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