CNOT1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CNOT1
Entrez Gene ID	23019
Full Name	CCR4-NOT transcription complex subunit 1
Synonyms	AD-005,CDC39,NOT1,NOT1H
General protein information	Preferred Names CCR4-NOT transcription complex subunit 1 Names CCR4-NOT transcription complex subunit 1 CCR4-associated factor 1 NOT1 (negative regulator of transcription 1, yeast) homolog adrenal gland protein AD-005 negative regulator of transcription subunit 1 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q21
Disorder MIM:	604917

mRNA and Protein(s)

mRNA	Protein	Name
NM_016284.4	NP_057368.3	CCR4-NOT transcription complex subunit 1 isoform a
NM_001265612.2	NP_001252541.1	CCR4-NOT transcription complex subunit 1 isoform c
NM_206999.2	NP_996882.1	CCR4-NOT transcription complex subunit 1 isoform b
NM_206999.3	NP_996882.1	CCR4-NOT transcription complex subunit 1 isoform b
NM_016284.5	NP_057368.3	CCR4-NOT transcription complex subunit 1 isoform a
NM_001265612.1	NP_001252541.1	CCR4-NOT transcription complex subunit 1 isoform c

Pathways from BioSystems

Homologies

Homo sapiens (human)	CNOT1	NP_057368.3
Mus musculus (house mouse)	Cnot1	NP_835179.1
Danio rerio (zebrafish)	cnot1	NP_001073420.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009057	XP_319808.4
Drosophila melanogaster (fruit fly)	Not1	NP_724798.3
Caenorhabditis elegans (roundworm)	let-711	NP_498516.2
Oryza sativa (rice)	Os10g0556801	NP_001176268.1
Pan troglodytes (chimpanzee)	CNOT1	XP_511007.2
Bos taurus (cattle)	CNOT1	NP_001192968.1
Gallus gallus (chicken)	CNOT1	NP_001264062.1
Rattus norvegicus (Norway rat)	Cnot1	NP_001128312.1
	CNOT1	XP_001102008.2
Canis lupus familiaris (dog)	CNOT1	XP_535279.4
Arabidopsis thaliana (thale cress)	AT1G02080	NP_171710.4
Xenopus tropicalis (tropical clawed frog)	cnot1	NP_001090658.1

Gene Ontology
Bibliography

Related articles in PubMed

The CCR4-NOT complex contributes to repression of Major Histocompatibility Complex class II transcription.
Rodr?guez-Gil A, Ritter O, Saul VV, Wilhelm J, Yang CY, Grosschedl R, Imai Y, Kuba K, Kracht M, Schmitz ML
Scientific reports7(1)3547(2017 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the CNOT1-LMNA-Hedgehog signaling pathway axis exerts an oncogenic role in osteosarcoma progression, which could be a potential target for gene therapy.
Title: CNOT1 cooperates with LMNA to aggravate osteosarcoma tumorigenesis through the Hedgehog signaling pathway.

we demonstrate that joint deletion of two short conserved motifs that bind UNR and DDX6 relieves repression of 4E-T-bound mRNA, in part reliant on the 4E-T-DDX6-CNOT1 axis.
Title: The DDX6-4E-T interaction mediates translational repression and P-body assembly.

miRNAs, AGOs, GW182, the CCR4-NOT complex, and DDX6/Me31B repress and degrade polyadenylated mRNA targets that are translated via scanning-independent mechanisms in both human and Drosophila melanogaster cells
Title: miRISC and the CCR4-NOT complex silence mRNA targets independently of 43S ribosomal scanning.

Single nucleotide polymorphism in CNOT1 gene is associated with osteosarcoma susceptibility.
Title: Polymorphisms in miRNA processing genes and their role in osteosarcoma risk.

CNOT1 facilitates recruitment of DDX6 to miRNA-targeted mRNAs, placing DDX6 as a downstream effector in the miRNA silencing pathway.
Title: Human DDX6 effects miRNA-mediated gene silencing via direct binding to CNOT1.

The following CNOT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CNOT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu07715	NM_206999.3 Latest version!	Homo sapiens CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu08018	NM_016284.5 Latest version!	Homo sapiens CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu08102	NM_001265612.2 Latest version!	Homo sapiens CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
View Old Accession Versions >>
OHu07715	NM_206999.2	Homo sapiens CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu08018	NM_016284.4	Homo sapiens CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu08102	NM_001265612.1	Homo sapiens CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CNOT1 ( NM_206999.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_996882.1 Homo sapiens CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant 2, mRNA.

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CloneID	OHu07715
Clone ID Related Accession (Same CDS sequence)	NM_206999.2 , NM_206999.3
Accession Version	NM_206999.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	CCR4-NOT transcription complex subunit 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB456343.1, BC040523.1, AC010287.9 and AC009118.11. On May 24, 2012 this sequence version replaced NM_206999.1. Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. The encoded isoform (b) is shorter and has a distinct C-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC040523.1, BX647815.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ATTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT 
CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTCCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTTG 
CTGATTACAA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC 
TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTGCTCAAA 
TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTGT TGAATTCTTC CAGCTCAGAT 
CTTAGAGGTT TCGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC 
ATTGACGCAG ACGTCAGTGG AAATCAAGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC 
CTACACCTCC TCCTCTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAA 
GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAAGA ACGCTGTCCC 
GTGGTGCTCG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTCATGCAA 
GAAGTAGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CGTGCAGTTT 
GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TGGGAATGAT GGCTCGAACT 
CATTCAGGAT TAACAGATGG CATTCCATTA CAGAGTATTT CTGCTCCGGG CAGTGGGATC 
TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC 
TTGATTGACG TTCTTAAAGA ACTGAATCCA AGTTTGAATT TCAAGGAAGT AACTTATGAA 
CTGGACCATC CTGGATTTCA AATTCGTGAC AGTAAAGGAC TTCATAATGT GGTTTATGGC 
ATTCAGAGGG GTTTGGGTAT GGAAGTGTTC CCAGTAGACC TCATATATAG ACCTTGGAAA 
CATGCTGAAG GCCAGCTCTC CTTCATTCAA CATTCCCTTA TAAATCCAGA GATCTTCTGT 
TTTGCTGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT 
GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTGAGGCTT 
GCAGAGGTTG GGCAGTATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT 
CCAGACATGC TGGTATTGGC CTTACTACAA ATTAACACCT CTTGGCATAC CTTGCGCCAT 
GAACTTATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ATCCTAACTC AGCTATTATT 
TTGCACTATG CATGGCATGG GCAGGGACAG TCTCCCTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTATGATC AGGCCAAATT GTCTCGAATA 
CTTGATGTGG CCCAGGACTT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGATAAGTGG 
CTCACAGATA AAATTCGAGA GCATGGGGAG CCTTTTATCC AGGCGTGTAT GACTTTTTTA 
AAGAGACGGT GTCCTTCTAT TTTGGGCGGA CTTGCCCCAG AAAAAGACCA GCCCAAAAGT 
GCTCAACTTC CTCCAGAAAC TTTGGCGACA ATGTTGGCCT GTCTGCAAGC TTGTGCAGGG 
AGTGTTTCTC AGGAGCTATC AGAAACTATC CTCACCATGG TAGCCAATTG CAGTAATGTT 
ATGAATAAGG CCAGACAACC ACCACCTGGA GTTATGCCAA AAGGACGTCC TCCTAGTGCT 
AGCAGCTTAG ATGCCATTTC TCCTGTTCAG ATTGACCCTC TTGCTGGAAT GACATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCAT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCC 
AATCAGACCA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT 
CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGTGCTC TTGGACTCCC TGCAGTGAAT 
AACGACCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAATCAGCC TACATTCCAG 
CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA CCAGCACTTT 
AGTAAAGAGA TAGATGATGA AGCAAACAGC TATTTCCAGC GAATATATAA TCATCCACCA 
CATCCAACCA TGTCTGTTGA TGAGGTATTA GAAATGCTGC AGAGATTTAA AGACTCTACT 
ATAAAGAGGG AACGAGAAGT ATTTAACTGT ATGCTAAGGA ACTTGTTTGA AGAATATCGT 
TTTTTTCCCC AGTATCCTGA TAAAGAGTTA CATATAACAG CCTGCCTATT TGGTGGTATA 
ATTGAGAAAG GACTGGTCAC TTACATGGCA CTAGGTCTGG CTCTACGATA TGTTCTTGAA 
GCCTTACGCA AGCCTTTTGG ATCCAAAATG TATTATTTCG GGATTGCTGC ACTAGATAGA 
TTTAAAAACA GATTGAAGGA CTATCCCCAG TATTGTCAGC ATTTGGCTTC TATCAGTCAC 
TTTATGCAAT TTCCACATCA TTTACAGGAG TATATTGAGT ATGGACAGCA GTCTAGAGAT 
CCTCCTGTGA AAATGCAAGG CTCTATCACA ACCCCTGGAA GTATTGCACT GGCTCAGGCC 
CAGGCTCAGG CCCAGGTTCC AGCAAAAGCT CCTCTTGCTG GTCAAGTTAG CACTATGGTA 
ACCACCTCAA CAACTACCAC TGTTGCTAAA ACGGTTACGG TCACCAGGCC AACTGGAGTC 
AGCTTTAAGA AAGATGTGCC ACCTTCTATT AATACTACAA ATATAGATAC GTTGCTTGTG 
GCCACAGATC AAACTGAGAG AATTGTGGAG CCCCCAGAAA ATATCCAGGA GAAAATTGCT 
TTTATTTTCA ATAATCTCTC ACAGTCAAAT ATGACACAAA AGGTTGAAGA GCTAAAGGAA 
ACGGTGAAAG AAGAATTTAT GCCTTGGGTT TCACAGTATC TGGTTATGAA GAGAGTCAGT 
ATTGAGCCAA ACTTTCATAG CCTGTATTCA AACTTCCTTG ACACGCTGAA GAATCCTGAA 
TTTAACAAGA TGGTTCTGAA TGAGACCTAC AGAAACATTA AAGTGCTCCT GACCTCTGAT 
AAAGCTGCAG CCAATTTCTC AGATCGTTCT TTGCTGAAGA ACTTGGGACA TTGGCTAGGA 
ATGATCACAT TAGCTAAAAA CAAACCCATC TTACACACTG ACTTGGATGT GAAATCATTG 
CTGCTAGAGG CTTATGTTAA AGGACAACAA GAATTGCTCT ATGTAGTGCC CTTTGTTGCC 
AAAGTCTTAG AATCTAGCAT TCGTAGTGTG GTTTTTAGGC CACCAAACCC TTGGACAATG 
GCAATTATGA ATGTATTAGC TGAGCTACAT CAGGAGCATG ACTTAAAGTT AAACTTGAAG 
TTTGAAATCG AGGTTCTCTG CAAGAACCTT GCATTAGACA TCAATGAGCT AAAACCTGGA 
AACCTCCTAA AGGATAAAGA TCGCCTGAAG AATTTAGATG AGCAACTCTC TGCTCCAAAG 
AAAGATGTCA AGCAGCCAGA AGAACTCCCT CCCATCACAA CCACAACAAC TTCTACTACA 
CCAGCTACCA ACACCACTTG TACAGCCACG GTTCCACCAC AGCCACAGTA CAGCTACCAC 
GACATCAATG TCTATTCCCT TGCGGGCTTG GCACCACACA TTACTCTGAA TCCAACAATT 
CCCTTGTTTC AGGCCCATCC ACAGTTGAAG CAGTGTGTGC GTCAGGCAAT TGAACGGGCT 
GTCCAGGAGC TGGTCCATCC TGTGGTGGAT CGATCAATTA AGATTGCCAT GACTACTTGT 
GAGCAAATAG TCAGGAAGGA TTTTGCCCTG GATTCGGAGG AATCTCGAAT GCGAATAGCA 
GCTCATCACA TGATGCGTAA CTTGACAGCT GGAATGGCTA TGATTACATG CAGGGAACCT 
TTGCTCATGA GCATATCTAC CAACTTAAAA AACAGTTTTG CCTCAGCCCT TCGTGTAAGT 
TGGCTATTTC CTTGGTATAG GTACAAAACG TATTACTGCT TGTCTGTAAT AATTTTTTTC 
TTTGTCTATA TATGGCACTG GGCGTTACCA CTTATTCTTA ATAATCACCA TATTTGTTTG 
ATGTCTTCCA TCATTTTAGA TTGTAATTCT GTGAGGCAAA GCATCATGTC TGTGTGTTTT 
TTTTTTTTTC TGTTATATTC TCAACACGAT GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996882.1
CDS	334..4989
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECALLITKPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVGYGFCASIEECRNIIVQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLHNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFSFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDPLAGMTSLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPVGGLGTGSLTGIGTGALGLPAVNNDPFVQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSTIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFGSKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFMQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTMVTTSTTTTVAKTVTVTRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENIQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSIRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDINELKPGNLLKDKDRLKNLDEQLSAPKKDVKQPEELPPITTTTTSTTPATNTTCTATVPPQPQYSYHDINVYSLAGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSISTNLKNSFASALRVSWLFPWYRYKTYYCLSVIIFFFVYIWHWALPLILNNHHICLMSSIILDCNSVRQSIMSVCFFFFLLYSQHDV

Target ORF information:

RefSeq Version	NM_206999.2
Organism	Homo sapiens(human)
Definition	Homo sapiens CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206999.2

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ATTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT 
CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTCCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTTG 
CTGATTACAA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC 
TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTGCTCAAA 
TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTGT TGAATTCTTC CAGCTCAGAT 
CTTAGAGGTT TCGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC 
ATTGACGCAG ACGTCAGTGG AAATCAAGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC 
CTACACCTCC TCCTCTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAA 
GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAAGA ACGCTGTCCC 
GTGGTGCTCG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTCATGCAA 
GAAGTAGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CGTGCAGTTT 
GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TGGGAATGAT GGCTCGAACT 
CATTCAGGAT TAACAGATGG CATTCCATTA CAGAGTATTT CTGCTCCGGG CAGTGGGATC 
TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC 
TTGATTGACG TTCTTAAAGA ACTGAATCCA AGTTTGAATT TCAAGGAAGT AACTTATGAA 
CTGGACCATC CTGGATTTCA AATTCGTGAC AGTAAAGGAC TTCATAATGT GGTTTATGGC 
ATTCAGAGGG GTTTGGGTAT GGAAGTGTTC CCAGTAGACC TCATATATAG ACCTTGGAAA 
CATGCTGAAG GCCAGCTCTC CTTCATTCAA CATTCCCTTA TAAATCCAGA GATCTTCTGT 
TTTGCTGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT 
GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTGAGGCTT 
GCAGAGGTTG GGCAGTATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT 
CCAGACATGC TGGTATTGGC CTTACTACAA ATTAACACCT CTTGGCATAC CTTGCGCCAT 
GAACTTATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ATCCTAACTC AGCTATTATT 
TTGCACTATG CATGGCATGG GCAGGGACAG TCTCCCTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTATGATC AGGCCAAATT GTCTCGAATA 
CTTGATGTGG CCCAGGACTT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGATAAGTGG 
CTCACAGATA AAATTCGAGA GCATGGGGAG CCTTTTATCC AGGCGTGTAT GACTTTTTTA 
AAGAGACGGT GTCCTTCTAT TTTGGGCGGA CTTGCCCCAG AAAAAGACCA GCCCAAAAGT 
GCTCAACTTC CTCCAGAAAC TTTGGCGACA ATGTTGGCCT GTCTGCAAGC TTGTGCAGGG 
AGTGTTTCTC AGGAGCTATC AGAAACTATC CTCACCATGG TAGCCAATTG CAGTAATGTT 
ATGAATAAGG CCAGACAACC ACCACCTGGA GTTATGCCAA AAGGACGTCC TCCTAGTGCT 
AGCAGCTTAG ATGCCATTTC TCCTGTTCAG ATTGACCCTC TTGCTGGAAT GACATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCAT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCC 
AATCAGACCA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT 
CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGTGCTC TTGGACTCCC TGCAGTGAAT 
AACGACCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAATCAGCC TACATTCCAG 
CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA CCAGCACTTT 
AGTAAAGAGA TAGATGATGA AGCAAACAGC TATTTCCAGC GAATATATAA TCATCCACCA 
CATCCAACCA TGTCTGTTGA TGAGGTATTA GAAATGCTGC AGAGATTTAA AGACTCTACT 
ATAAAGAGGG AACGAGAAGT ATTTAACTGT ATGCTAAGGA ACTTGTTTGA AGAATATCGT 
TTTTTTCCCC AGTATCCTGA TAAAGAGTTA CATATAACAG CCTGCCTATT TGGTGGTATA 
ATTGAGAAAG GACTGGTCAC TTACATGGCA CTAGGTCTGG CTCTACGATA TGTTCTTGAA 
GCCTTACGCA AGCCTTTTGG ATCCAAAATG TATTATTTCG GGATTGCTGC ACTAGATAGA 
TTTAAAAACA GATTGAAGGA CTATCCCCAG TATTGTCAGC ATTTGGCTTC TATCAGTCAC 
TTTATGCAAT TTCCACATCA TTTACAGGAG TATATTGAGT ATGGACAGCA GTCTAGAGAT 
CCTCCTGTGA AAATGCAAGG CTCTATCACA ACCCCTGGAA GTATTGCACT GGCTCAGGCC 
CAGGCTCAGG CCCAGGTTCC AGCAAAAGCT CCTCTTGCTG GTCAAGTTAG CACTATGGTA 
ACCACCTCAA CAACTACCAC TGTTGCTAAA ACGGTTACGG TCACCAGGCC AACTGGAGTC 
AGCTTTAAGA AAGATGTGCC ACCTTCTATT AATACTACAA ATATAGATAC GTTGCTTGTG 
GCCACAGATC AAACTGAGAG AATTGTGGAG CCCCCAGAAA ATATCCAGGA GAAAATTGCT 
TTTATTTTCA ATAATCTCTC ACAGTCAAAT ATGACACAAA AGGTTGAAGA GCTAAAGGAA 
ACGGTGAAAG AAGAATTTAT GCCTTGGGTT TCACAGTATC TGGTTATGAA GAGAGTCAGT 
ATTGAGCCAA ACTTTCATAG CCTGTATTCA AACTTCCTTG ACACGCTGAA GAATCCTGAA 
TTTAACAAGA TGGTTCTGAA TGAGACCTAC AGAAACATTA AAGTGCTCCT GACCTCTGAT 
AAAGCTGCAG CCAATTTCTC AGATCGTTCT TTGCTGAAGA ACTTGGGACA TTGGCTAGGA 
ATGATCACAT TAGCTAAAAA CAAACCCATC TTACACACTG ACTTGGATGT GAAATCATTG 
CTGCTAGAGG CTTATGTTAA AGGACAACAA GAATTGCTCT ATGTAGTGCC CTTTGTTGCC 
AAAGTCTTAG AATCTAGCAT TCGTAGTGTG GTTTTTAGGC CACCAAACCC TTGGACAATG 
GCAATTATGA ATGTATTAGC TGAGCTACAT CAGGAGCATG ACTTAAAGTT AAACTTGAAG 
TTTGAAATCG AGGTTCTCTG CAAGAACCTT GCATTAGACA TCAATGAGCT AAAACCTGGA 
AACCTCCTAA AGGATAAAGA TCGCCTGAAG AATTTAGATG AGCAACTCTC TGCTCCAAAG 
AAAGATGTCA AGCAGCCAGA AGAACTCCCT CCCATCACAA CCACAACAAC TTCTACTACA 
CCAGCTACCA ACACCACTTG TACAGCCACG GTTCCACCAC AGCCACAGTA CAGCTACCAC 
GACATCAATG TCTATTCCCT TGCGGGCTTG GCACCACACA TTACTCTGAA TCCAACAATT 
CCCTTGTTTC AGGCCCATCC ACAGTTGAAG CAGTGTGTGC GTCAGGCAAT TGAACGGGCT 
GTCCAGGAGC TGGTCCATCC TGTGGTGGAT CGATCAATTA AGATTGCCAT GACTACTTGT 
GAGCAAATAG TCAGGAAGGA TTTTGCCCTG GATTCGGAGG AATCTCGAAT GCGAATAGCA 
GCTCATCACA TGATGCGTAA CTTGACAGCT GGAATGGCTA TGATTACATG CAGGGAACCT 
TTGCTCATGA GCATATCTAC CAACTTAAAA AACAGTTTTG CCTCAGCCCT TCGTGTAAGT 
TGGCTATTTC CTTGGTATAG GTACAAAACG TATTACTGCT TGTCTGTAAT AATTTTTTTC 
TTTGTCTATA TATGGCACTG GGCGTTACCA CTTATTCTTA ATAATCACCA TATTTGTTTG 
ATGTCTTCCA TCATTTTAGA TTGTAATTCT GTGAGGCAAA GCATCATGTC TGTGTGTTTT 
TTTTTTTTTC TGTTATATTC TCAACACGAT GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( NM_206999.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_996882.1 Homo sapiens CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu07715
Clone ID Related Accession (Same CDS sequence)	NM_206999.2 , NM_206999.3
Accession Version	NM_206999.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-18
Organism	Homo sapiens(human)
Product	CCR4-NOT transcription complex subunit 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC040523.1, AC010287.9 and AC009118.11. On May 31, 2019 this sequence version replaced NM_206999.2. Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. The encoded isoform (b) is shorter and has a distinct C-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX647815.1, SRR1803616.149564.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ATTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT 
CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTCCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTTG 
CTGATTACAA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC 
TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTGCTCAAA 
TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTGT TGAATTCTTC CAGCTCAGAT 
CTTAGAGGTT TCGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC 
ATTGACGCAG ACGTCAGTGG AAATCAAGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC 
CTACACCTCC TCCTCTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAA 
GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAAGA ACGCTGTCCC 
GTGGTGCTCG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTCATGCAA 
GAAGTAGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CGTGCAGTTT 
GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TGGGAATGAT GGCTCGAACT 
CATTCAGGAT TAACAGATGG CATTCCATTA CAGAGTATTT CTGCTCCGGG CAGTGGGATC 
TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC 
TTGATTGACG TTCTTAAAGA ACTGAATCCA AGTTTGAATT TCAAGGAAGT AACTTATGAA 
CTGGACCATC CTGGATTTCA AATTCGTGAC AGTAAAGGAC TTCATAATGT GGTTTATGGC 
ATTCAGAGGG GTTTGGGTAT GGAAGTGTTC CCAGTAGACC TCATATATAG ACCTTGGAAA 
CATGCTGAAG GCCAGCTCTC CTTCATTCAA CATTCCCTTA TAAATCCAGA GATCTTCTGT 
TTTGCTGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT 
GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTGAGGCTT 
GCAGAGGTTG GGCAGTATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT 
CCAGACATGC TGGTATTGGC CTTACTACAA ATTAACACCT CTTGGCATAC CTTGCGCCAT 
GAACTTATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ATCCTAACTC AGCTATTATT 
TTGCACTATG CATGGCATGG GCAGGGACAG TCTCCCTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTATGATC AGGCCAAATT GTCTCGAATA 
CTTGATGTGG CCCAGGACTT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGATAAGTGG 
CTCACAGATA AAATTCGAGA GCATGGGGAG CCTTTTATCC AGGCGTGTAT GACTTTTTTA 
AAGAGACGGT GTCCTTCTAT TTTGGGCGGA CTTGCCCCAG AAAAAGACCA GCCCAAAAGT 
GCTCAACTTC CTCCAGAAAC TTTGGCGACA ATGTTGGCCT GTCTGCAAGC TTGTGCAGGG 
AGTGTTTCTC AGGAGCTATC AGAAACTATC CTCACCATGG TAGCCAATTG CAGTAATGTT 
ATGAATAAGG CCAGACAACC ACCACCTGGA GTTATGCCAA AAGGACGTCC TCCTAGTGCT 
AGCAGCTTAG ATGCCATTTC TCCTGTTCAG ATTGACCCTC TTGCTGGAAT GACATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCAT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCC 
AATCAGACCA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT 
CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGTGCTC TTGGACTCCC TGCAGTGAAT 
AACGACCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAATCAGCC TACATTCCAG 
CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA CCAGCACTTT 
AGTAAAGAGA TAGATGATGA AGCAAACAGC TATTTCCAGC GAATATATAA TCATCCACCA 
CATCCAACCA TGTCTGTTGA TGAGGTATTA GAAATGCTGC AGAGATTTAA AGACTCTACT 
ATAAAGAGGG AACGAGAAGT ATTTAACTGT ATGCTAAGGA ACTTGTTTGA AGAATATCGT 
TTTTTTCCCC AGTATCCTGA TAAAGAGTTA CATATAACAG CCTGCCTATT TGGTGGTATA 
ATTGAGAAAG GACTGGTCAC TTACATGGCA CTAGGTCTGG CTCTACGATA TGTTCTTGAA 
GCCTTACGCA AGCCTTTTGG ATCCAAAATG TATTATTTCG GGATTGCTGC ACTAGATAGA 
TTTAAAAACA GATTGAAGGA CTATCCCCAG TATTGTCAGC ATTTGGCTTC TATCAGTCAC 
TTTATGCAAT TTCCACATCA TTTACAGGAG TATATTGAGT ATGGACAGCA GTCTAGAGAT 
CCTCCTGTGA AAATGCAAGG CTCTATCACA ACCCCTGGAA GTATTGCACT GGCTCAGGCC 
CAGGCTCAGG CCCAGGTTCC AGCAAAAGCT CCTCTTGCTG GTCAAGTTAG CACTATGGTA 
ACCACCTCAA CAACTACCAC TGTTGCTAAA ACGGTTACGG TCACCAGGCC AACTGGAGTC 
AGCTTTAAGA AAGATGTGCC ACCTTCTATT AATACTACAA ATATAGATAC GTTGCTTGTG 
GCCACAGATC AAACTGAGAG AATTGTGGAG CCCCCAGAAA ATATCCAGGA GAAAATTGCT 
TTTATTTTCA ATAATCTCTC ACAGTCAAAT ATGACACAAA AGGTTGAAGA GCTAAAGGAA 
ACGGTGAAAG AAGAATTTAT GCCTTGGGTT TCACAGTATC TGGTTATGAA GAGAGTCAGT 
ATTGAGCCAA ACTTTCATAG CCTGTATTCA AACTTCCTTG ACACGCTGAA GAATCCTGAA 
TTTAACAAGA TGGTTCTGAA TGAGACCTAC AGAAACATTA AAGTGCTCCT GACCTCTGAT 
AAAGCTGCAG CCAATTTCTC AGATCGTTCT TTGCTGAAGA ACTTGGGACA TTGGCTAGGA 
ATGATCACAT TAGCTAAAAA CAAACCCATC TTACACACTG ACTTGGATGT GAAATCATTG 
CTGCTAGAGG CTTATGTTAA AGGACAACAA GAATTGCTCT ATGTAGTGCC CTTTGTTGCC 
AAAGTCTTAG AATCTAGCAT TCGTAGTGTG GTTTTTAGGC CACCAAACCC TTGGACAATG 
GCAATTATGA ATGTATTAGC TGAGCTACAT CAGGAGCATG ACTTAAAGTT AAACTTGAAG 
TTTGAAATCG AGGTTCTCTG CAAGAACCTT GCATTAGACA TCAATGAGCT AAAACCTGGA 
AACCTCCTAA AGGATAAAGA TCGCCTGAAG AATTTAGATG AGCAACTCTC TGCTCCAAAG 
AAAGATGTCA AGCAGCCAGA AGAACTCCCT CCCATCACAA CCACAACAAC TTCTACTACA 
CCAGCTACCA ACACCACTTG TACAGCCACG GTTCCACCAC AGCCACAGTA CAGCTACCAC 
GACATCAATG TCTATTCCCT TGCGGGCTTG GCACCACACA TTACTCTGAA TCCAACAATT 
CCCTTGTTTC AGGCCCATCC ACAGTTGAAG CAGTGTGTGC GTCAGGCAAT TGAACGGGCT 
GTCCAGGAGC TGGTCCATCC TGTGGTGGAT CGATCAATTA AGATTGCCAT GACTACTTGT 
GAGCAAATAG TCAGGAAGGA TTTTGCCCTG GATTCGGAGG AATCTCGAAT GCGAATAGCA 
GCTCATCACA TGATGCGTAA CTTGACAGCT GGAATGGCTA TGATTACATG CAGGGAACCT 
TTGCTCATGA GCATATCTAC CAACTTAAAA AACAGTTTTG CCTCAGCCCT TCGTGTAAGT 
TGGCTATTTC CTTGGTATAG GTACAAAACG TATTACTGCT TGTCTGTAAT AATTTTTTTC 
TTTGTCTATA TATGGCACTG GGCGTTACCA CTTATTCTTA ATAATCACCA TATTTGTTTG 
ATGTCTTCCA TCATTTTAGA TTGTAATTCT GTGAGGCAAA GCATCATGTC TGTGTGTTTT 
TTTTTTTTTC TGTTATATTC TCAACACGAT GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996882.1
CDS	274..4929
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECALLITKPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVGYGFCASIEECRNIIVQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLHNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFSFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDPLAGMTSLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPVGGLGTGSLTGIGTGALGLPAVNNDPFVQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSTIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFGSKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFMQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTMVTTSTTTTVAKTVTVTRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENIQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSIRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDINELKPGNLLKDKDRLKNLDEQLSAPKKDVKQPEELPPITTTTTSTTPATNTTCTATVPPQPQYSYHDINVYSLAGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSISTNLKNSFASALRVSWLFPWYRYKTYYCLSVIIFFFVYIWHWALPLILNNHHICLMSSIILDCNSVRQSIMSVCFFFFLLYSQHDV

Target ORF information:

RefSeq Version	NM_206999.3
Organism	Homo sapiens(human)
Definition	Homo sapiens CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206999.3

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ATTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT 
CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTCCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTTG 
CTGATTACAA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC 
TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTGCTCAAA 
TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTGT TGAATTCTTC CAGCTCAGAT 
CTTAGAGGTT TCGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC 
ATTGACGCAG ACGTCAGTGG AAATCAAGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC 
CTACACCTCC TCCTCTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAA 
GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAAGA ACGCTGTCCC 
GTGGTGCTCG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTCATGCAA 
GAAGTAGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CGTGCAGTTT 
GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TGGGAATGAT GGCTCGAACT 
CATTCAGGAT TAACAGATGG CATTCCATTA CAGAGTATTT CTGCTCCGGG CAGTGGGATC 
TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC 
TTGATTGACG TTCTTAAAGA ACTGAATCCA AGTTTGAATT TCAAGGAAGT AACTTATGAA 
CTGGACCATC CTGGATTTCA AATTCGTGAC AGTAAAGGAC TTCATAATGT GGTTTATGGC 
ATTCAGAGGG GTTTGGGTAT GGAAGTGTTC CCAGTAGACC TCATATATAG ACCTTGGAAA 
CATGCTGAAG GCCAGCTCTC CTTCATTCAA CATTCCCTTA TAAATCCAGA GATCTTCTGT 
TTTGCTGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT 
GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTGAGGCTT 
GCAGAGGTTG GGCAGTATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT 
CCAGACATGC TGGTATTGGC CTTACTACAA ATTAACACCT CTTGGCATAC CTTGCGCCAT 
GAACTTATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ATCCTAACTC AGCTATTATT 
TTGCACTATG CATGGCATGG GCAGGGACAG TCTCCCTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTATGATC AGGCCAAATT GTCTCGAATA 
CTTGATGTGG CCCAGGACTT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGATAAGTGG 
CTCACAGATA AAATTCGAGA GCATGGGGAG CCTTTTATCC AGGCGTGTAT GACTTTTTTA 
AAGAGACGGT GTCCTTCTAT TTTGGGCGGA CTTGCCCCAG AAAAAGACCA GCCCAAAAGT 
GCTCAACTTC CTCCAGAAAC TTTGGCGACA ATGTTGGCCT GTCTGCAAGC TTGTGCAGGG 
AGTGTTTCTC AGGAGCTATC AGAAACTATC CTCACCATGG TAGCCAATTG CAGTAATGTT 
ATGAATAAGG CCAGACAACC ACCACCTGGA GTTATGCCAA AAGGACGTCC TCCTAGTGCT 
AGCAGCTTAG ATGCCATTTC TCCTGTTCAG ATTGACCCTC TTGCTGGAAT GACATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCAT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCC 
AATCAGACCA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT 
CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGTGCTC TTGGACTCCC TGCAGTGAAT 
AACGACCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAATCAGCC TACATTCCAG 
CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA CCAGCACTTT 
AGTAAAGAGA TAGATGATGA AGCAAACAGC TATTTCCAGC GAATATATAA TCATCCACCA 
CATCCAACCA TGTCTGTTGA TGAGGTATTA GAAATGCTGC AGAGATTTAA AGACTCTACT 
ATAAAGAGGG AACGAGAAGT ATTTAACTGT ATGCTAAGGA ACTTGTTTGA AGAATATCGT 
TTTTTTCCCC AGTATCCTGA TAAAGAGTTA CATATAACAG CCTGCCTATT TGGTGGTATA 
ATTGAGAAAG GACTGGTCAC TTACATGGCA CTAGGTCTGG CTCTACGATA TGTTCTTGAA 
GCCTTACGCA AGCCTTTTGG ATCCAAAATG TATTATTTCG GGATTGCTGC ACTAGATAGA 
TTTAAAAACA GATTGAAGGA CTATCCCCAG TATTGTCAGC ATTTGGCTTC TATCAGTCAC 
TTTATGCAAT TTCCACATCA TTTACAGGAG TATATTGAGT ATGGACAGCA GTCTAGAGAT 
CCTCCTGTGA AAATGCAAGG CTCTATCACA ACCCCTGGAA GTATTGCACT GGCTCAGGCC 
CAGGCTCAGG CCCAGGTTCC AGCAAAAGCT CCTCTTGCTG GTCAAGTTAG CACTATGGTA 
ACCACCTCAA CAACTACCAC TGTTGCTAAA ACGGTTACGG TCACCAGGCC AACTGGAGTC 
AGCTTTAAGA AAGATGTGCC ACCTTCTATT AATACTACAA ATATAGATAC GTTGCTTGTG 
GCCACAGATC AAACTGAGAG AATTGTGGAG CCCCCAGAAA ATATCCAGGA GAAAATTGCT 
TTTATTTTCA ATAATCTCTC ACAGTCAAAT ATGACACAAA AGGTTGAAGA GCTAAAGGAA 
ACGGTGAAAG AAGAATTTAT GCCTTGGGTT TCACAGTATC TGGTTATGAA GAGAGTCAGT 
ATTGAGCCAA ACTTTCATAG CCTGTATTCA AACTTCCTTG ACACGCTGAA GAATCCTGAA 
TTTAACAAGA TGGTTCTGAA TGAGACCTAC AGAAACATTA AAGTGCTCCT GACCTCTGAT 
AAAGCTGCAG CCAATTTCTC AGATCGTTCT TTGCTGAAGA ACTTGGGACA TTGGCTAGGA 
ATGATCACAT TAGCTAAAAA CAAACCCATC TTACACACTG ACTTGGATGT GAAATCATTG 
CTGCTAGAGG CTTATGTTAA AGGACAACAA GAATTGCTCT ATGTAGTGCC CTTTGTTGCC 
AAAGTCTTAG AATCTAGCAT TCGTAGTGTG GTTTTTAGGC CACCAAACCC TTGGACAATG 
GCAATTATGA ATGTATTAGC TGAGCTACAT CAGGAGCATG ACTTAAAGTT AAACTTGAAG 
TTTGAAATCG AGGTTCTCTG CAAGAACCTT GCATTAGACA TCAATGAGCT AAAACCTGGA 
AACCTCCTAA AGGATAAAGA TCGCCTGAAG AATTTAGATG AGCAACTCTC TGCTCCAAAG 
AAAGATGTCA AGCAGCCAGA AGAACTCCCT CCCATCACAA CCACAACAAC TTCTACTACA 
CCAGCTACCA ACACCACTTG TACAGCCACG GTTCCACCAC AGCCACAGTA CAGCTACCAC 
GACATCAATG TCTATTCCCT TGCGGGCTTG GCACCACACA TTACTCTGAA TCCAACAATT 
CCCTTGTTTC AGGCCCATCC ACAGTTGAAG CAGTGTGTGC GTCAGGCAAT TGAACGGGCT 
GTCCAGGAGC TGGTCCATCC TGTGGTGGAT CGATCAATTA AGATTGCCAT GACTACTTGT 
GAGCAAATAG TCAGGAAGGA TTTTGCCCTG GATTCGGAGG AATCTCGAAT GCGAATAGCA 
GCTCATCACA TGATGCGTAA CTTGACAGCT GGAATGGCTA TGATTACATG CAGGGAACCT 
TTGCTCATGA GCATATCTAC CAACTTAAAA AACAGTTTTG CCTCAGCCCT TCGTGTAAGT 
TGGCTATTTC CTTGGTATAG GTACAAAACG TATTACTGCT TGTCTGTAAT AATTTTTTTC 
TTTGTCTATA TATGGCACTG GGCGTTACCA CTTATTCTTA ATAATCACCA TATTTGTTTG 
ATGTCTTCCA TCATTTTAGA TTGTAATTCT GTGAGGCAAA GCATCATGTC TGTGTGTTTT 
TTTTTTTTTC TGTTATATTC TCAACACGAT GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( NM_016284.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057368.3 Homo sapiens CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu08018
Clone ID Related Accession (Same CDS sequence)	NM_016284.4 , NM_016284.5
Accession Version	NM_016284.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-11
Organism	Homo sapiens(human)
Product	CCR4-NOT transcription complex subunit 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB456343.1, AL833549.1, AC010287.9, BX537840.1, AL117492.1, AC009118.11 and BM023419.1. On May 24, 2012 this sequence version replaced NM_016284.3. Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.37693.1, SRR1803613.169791.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ATTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT 
CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTCCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTTG 
CTGATTACAA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC 
TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTGCTCAAA 
TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTGT TGAATTCTTC CAGCTCAGAT 
CTTAGAGGTT TCGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC 
ATTGACGCAG ACGTCAGTGG AAATCAAGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC 
CTACACCTCC TCCTCTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAA 
GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAAGA ACGCTGTCCC 
GTGGTGCTCG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTCATGCAA 
GAAGTAGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CGTGCAGTTT 
GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TGGGAATGAT GGCTCGAACT 
CATTCAGGAT TAACAGATGG CATTCCATTA CAGAGTATTT CTGCTCCGGG CAGTGGGATC 
TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC 
TTGATTGACG TTCTTAAAGA ACTGAATCCA AGTTTGAATT TCAAGGAAGT AACTTATGAA 
CTGGACCATC CTGGATTTCA AATTCGTGAC AGTAAAGGAC TTCATAATGT GGTTTATGGC 
ATTCAGAGGG GTTTGGGTAT GGAAGTGTTC CCAGTAGACC TCATATATAG ACCTTGGAAA 
CATGCTGAAG GCCAGCTCTC CTTCATTCAA CATTCCCTTA TAAATCCAGA GATCTTCTGT 
TTTGCTGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT 
GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTGAGGCTT 
GCAGAGGTTG GGCAGTATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT 
CCAGACATGC TGGTATTGGC CTTACTACAA ATTAACACCT CTTGGCATAC CTTGCGCCAT 
GAACTTATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ATCCTAACTC AGCTATTATT 
TTGCACTATG CATGGCATGG GCAGGGACAG TCTCCCTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTATGATC AGGCCAAATT GTCTCGAATA 
CTTGATGTGG CCCAGGACTT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGATAAGTGG 
CTCACAGATA AAATTCGAGA GCATGGGGAG CCTTTTATCC AGGCGTGTAT GACTTTTTTA 
AAGAGACGGT GTCCTTCTAT TTTGGGCGGA CTTGCCCCAG AAAAAGACCA GCCCAAAAGT 
GCTCAACTTC CTCCAGAAAC TTTGGCGACA ATGTTGGCCT GTCTGCAAGC TTGTGCAGGG 
AGTGTTTCTC AGGAGCTATC AGAAACTATC CTCACCATGG TAGCCAATTG CAGTAATGTT 
ATGAATAAGG CCAGACAACC ACCACCTGGA GTTATGCCAA AAGGACGTCC TCCTAGTGCT 
AGCAGCTTAG ATGCCATTTC TCCTGTTCAG ATTGACCCTC TTGCTGGAAT GACATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCAT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCC 
AATCAGACCA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT 
CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGTGCTC TTGGACTCCC TGCAGTGAAT 
AACGACCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAATCAGCC TACATTCCAG 
CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA CCAGCACTTT 
AGTAAAGAGA TAGATGATGA AGCAAACAGC TATTTCCAGC GAATATATAA TCATCCACCA 
CATCCAACCA TGTCTGTTGA TGAGGTATTA GAAATGCTGC AGAGATTTAA AGACTCTACT 
ATAAAGAGGG AACGAGAAGT ATTTAACTGT ATGCTAAGGA ACTTGTTTGA AGAATATCGT 
TTTTTTCCCC AGTATCCTGA TAAAGAGTTA CATATAACAG CCTGCCTATT TGGTGGTATA 
ATTGAGAAAG GACTGGTCAC TTACATGGCA CTAGGTCTGG CTCTACGATA TGTTCTTGAA 
GCCTTACGCA AGCCTTTTGG ATCCAAAATG TATTATTTCG GGATTGCTGC ACTAGATAGA 
TTTAAAAACA GATTGAAGGA CTATCCCCAG TATTGTCAGC ATTTGGCTTC TATCAGTCAC 
TTTATGCAAT TTCCACATCA TTTACAGGAG TATATTGAGT ATGGACAGCA GTCTAGAGAT 
CCTCCTGTGA AAATGCAAGG CTCTATCACA ACCCCTGGAA GTATTGCACT GGCTCAGGCC 
CAGGCTCAGG CCCAGGTTCC AGCAAAAGCT CCTCTTGCTG GTCAAGTTAG CACTATGGTA 
ACCACCTCAA CAACTACCAC TGTTGCTAAA ACGGTTACGG TCACCAGGCC AACTGGAGTC 
AGCTTTAAGA AAGATGTGCC ACCTTCTATT AATACTACAA ATATAGATAC GTTGCTTGTG 
GCCACAGATC AAACTGAGAG AATTGTGGAG CCCCCAGAAA ATATCCAGGA GAAAATTGCT 
TTTATTTTCA ATAATCTCTC ACAGTCAAAT ATGACACAAA AGGTTGAAGA GCTAAAGGAA 
ACGGTGAAAG AAGAATTTAT GCCTTGGGTT TCACAGTATC TGGTTATGAA GAGAGTCAGT 
ATTGAGCCAA ACTTTCATAG CCTGTATTCA AACTTCCTTG ACACGCTGAA GAATCCTGAA 
TTTAACAAGA TGGTTCTGAA TGAGACCTAC AGAAACATTA AAGTGCTCCT GACCTCTGAT 
AAAGCTGCAG CCAATTTCTC AGATCGTTCT TTGCTGAAGA ACTTGGGACA TTGGCTAGGA 
ATGATCACAT TAGCTAAAAA CAAACCCATC TTACACACTG ACTTGGATGT GAAATCATTG 
CTGCTAGAGG CTTATGTTAA AGGACAACAA GAATTGCTCT ATGTAGTGCC CTTTGTTGCC 
AAAGTCTTAG AATCTAGCAT TCGTAGTGTG GTTTTTAGGC CACCAAACCC TTGGACAATG 
GCAATTATGA ATGTATTAGC TGAGCTACAT CAGGAGCATG ACTTAAAGTT AAACTTGAAG 
TTTGAAATCG AGGTTCTCTG CAAGAACCTT GCATTAGACA TCAATGAGCT AAAACCTGGA 
AACCTCCTAA AGGATAAAGA TCGCCTGAAG AATTTAGATG AGCAACTCTC TGCTCCAAAG 
AAAGATGTCA AGCAGCCAGA AGAACTCCCT CCCATCACAA CCACAACAAC TTCTACTACA 
CCAGCTACCA ACACCACTTG TACAGCCACG GTTCCACCAC AGCCACAGTA CAGCTACCAC 
GACATCAATG TCTATTCCCT TGCGGGCTTG GCACCACACA TTACTCTGAA TCCAACAATT 
CCCTTGTTTC AGGCCCATCC ACAGTTGAAG CAGTGTGTGC GTCAGGCAAT TGAACGGGCT 
GTCCAGGAGC TGGTCCATCC TGTGGTGGAT CGATCAATTA AGATTGCCAT GACTACTTGT 
GAGCAAATAG TCAGGAAGGA TTTTGCCCTG GATTCGGAGG AATCTCGAAT GCGAATAGCA 
GCTCATCACA TGATGCGTAA CTTGACAGCT GGAATGGCTA TGATTACATG CAGGGAACCT 
TTGCTCATGA GCATATCTAC CAACTTAAAA AACAGTTTTG CCTCAGCCCT TCGTACTGCT 
TCCCCACAAC AAAGAGAAAT GATGGATCAG GCAGCTGCTC AATTAGCTCA GGACAATTGT 
GAGTTGGCTT GCTGTTTTAT TCAGAAGACT GCAGTAGAAA AAGCAGGCCC TGAGATGGAC 
AAGAGATTAG CAACTGAATT TGAGCTGAGA AAACATGCTA GGCAAGAAGG ACGCAGATAC 
TGTGATCCTG TTGTTTTAAC ATATCAAGCT GAACGGATGC CAGAGCAAAT CAGGCTGAAA 
GTTGGTGGTG TGGACCCAAA GCAGTTGGCT GTTTACGAAG AGTTTGCACG CAATGTTCCT 
GGCTTCTTAC CTACAAATGA CTTAAGTCAG CCCACGGGAT TTTTAGCCCA GCCCATGAAG 
CAAGCTTGGG CAACAGATGA TGTAGCTCAG ATTTATGATA AGTGTATTAC AGAACTGGAG 
CAACATCTAC ATGCCATCCC ACCAACTTTG GCCATGAACC CTCAAGCTCA GGCTCTTCGA 
AGTCTCTTGG AGGTTGTAGT TTTATCTCGA AACTCTCGGG ATGCCATAGC TGCTCTTGGA 
TTGCTCCAAA AGGCTGTAGA GGGCTTACTA GATGCCACAA GTGGTGCTGA TGCTGACCTT 
CTGCTGCGCT ACAGGGAATG CCACCTCTTG GTCCTAAAAG CTCTGCAGGA TGGCCGGGCA 
TATGGGTCTC CATGGTGCAA CAAACAGATC ACAAGGTGCC TAATTGAATG TCGAGATGAA 
TATAAATATA ATGTGGAGGC TGTGGAGCTG CTAATTCGCA ATCATTTGGT TAATATGCAG 
CAGTATGATC TTCACCTAGC GCAGTCAATG GAGAATGGCT TAAACTACAT GGCTGTGGCA 
TTTGCTATGC AGTTAGTAAA AATCCTGCTG GTGGATGAAA GGAGTGTTGC TCATGTTACT 
GAGGCAGATC TGTTCCACAC CATTGAAACC CTCATGAGGA TTAATGCTCA TTCCAGAGGC 
AATGCTCCAG AAGGATTGCC CCAGCTGATG GAAGTAGTGC GATCCAACTA TGAAGCAATG 
ATTGATCGTG CTCATGGAGG CCCAAACTTT ATGATGCATT CTGGGATCTC TCAAGCCTCA 
GAGTATGATG ACCCTCCAGG CCTGAGGGAG AAGGCAGAGT ATCTTCTGAG GGAATGGGTG 
AATCTCTACC ATTCAGCAGC AGCTGGCCGC GACAGTACCA AAGCTTTCTC TGCATTTGTT 
GGACAGATGC ACCAGCAAGG AATACTGAAG ACCGATGATC TCATAACAAG GTTCTTTCGT 
CTGTGTACTG AAATGTGTGT TGAAATCAGT TACCGTGCTC AGGCTGAGCA GCAGCACAAT 
CCTGCTGCCA ATCCCACCAT GATCCGAGCC AAGTGCTATC ACAACCTGGA TGCCTTTGTT 
CGACTCATTG CACTGCTCGT GAAACACTCA GGGGAGGCCA CCAACACTGT CACAAAGATT 
AATCTGCTGA ACAAGGTCCT TGGTATAGTA GTGGGAGTTC TCCTTCAGGA TCATGATGTT 
CGTCAGAGTG AATTTCAGCA ACTTCCCTAC CATCGAATTT TTATCATGCT TCTCTTGGAA 
CTCAATGCAC CTGAGCATGT GTTGGAAACC ATTAATTTCC AGACACTTAC AGCTTTCTGC 
AATACATTCC ACATCTTGAG GCCTACCAAA GCTCCTGGCT TTGTATATGC CTGGCTTGAA 
CTGATTTCCC ATCGGATATT TATTGCAAGA ATGCTGGCAC ATACGCCACA GCAGAAGGGG 
TGGCCTATGT ATGCACAGCT ACTGATTGAT TTATTCAAAT ATTTAGCGCC TTTCCTTAGA 
AATGTGGAAC TCACCAAACC TATGCAAATC CTCTACAAGG GCACTTTAAG AGTGCTGCTG 
GTTCTTTTGC ATGATTTCCC AGAGTTCCTT TGTGATTACC ATTATGGGTT CTGTGATGTG 
ATCCCACCTA ATTGTATCCA GTTAAGAAAT TTGATCCTGA GTGCCTTTCC AAGAAACATG 
AGGCTCCCCG ACCCATTCAC TCCTAATCTA AAGGTGGACA TGTTGAGTGA AATTAACATT 
GCTCCCCGGA TTCTCACCAA TTTCACTGGA GTAATGCCAC CTCAGTTCAA AAAGGATTTG 
GATTCCTATC TTAAAACTCG ATCACCAGTC ACTTTCCTGT CTGATCTGCG CAGCAACCTA 
CAGGTATCCA ATGAACCTGG GAATCGCTAC AACCTCCAGC TCATCAATGC ACTGGTGCTC 
TATGTCGGGA CTCAGGCCAT TGCGCACATC CACAACAAGG GCAGCACACC TTCAATGAGC 
ACCATCACTC ACTCAGCACA CATGGATATC TTCCAGAATT TGGCTGTGGA CTTGGACACT 
GAGGGTCGCT ATCTCTTTTT GAATGCAATT GCAAATCAGC TCCGGTACCC AAATAGCCAC 
ACTCACTACT TCAGTTGCAC CATGCTGTAC CTTTTTGCAG AGGCCAATAC GGAAGCCATC 
CAAGAACAGA TCACAAGAGT TCTCTTGGAA CGGTTGATTG TAAATAGGCC ACATCCTTGG 
GGTCTTCTTA TTACCTTCAT TGAGCTGATT AAAAACCCAG CGTTTAAGTT CTGGAACCAT 
GAATTTGTAC ACTGTGCCCC AGAAATCGAA AAGTTATTCC AGTCGGTCGC ACAGTGCTGC 
ATGGGACAGA AGCAGGCCCA GCAAGTAATG GAAGGGACAG GTGCCAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057368.3
CDS	334..7464
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECALLITKPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVGYGFCASIEECRNIIVQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLHNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFSFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDPLAGMTSLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPVGGLGTGSLTGIGTGALGLPAVNNDPFVQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSTIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFGSKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFMQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTMVTTSTTTTVAKTVTVTRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENIQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSIRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDINELKPGNLLKDKDRLKNLDEQLSAPKKDVKQPEELPPITTTTTSTTPATNTTCTATVPPQPQYSYHDINVYSLAGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSISTNLKNSFASALRTASPQQREMMDQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLSQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPTLAMNPQAQALRSLLEVVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERSVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRSNYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQSEFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNLQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	NM_016284.4
Organism	Homo sapiens(human)
Definition	Homo sapiens CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016284.4

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ATTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT 
CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTCCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTTG 
CTGATTACAA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC 
TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTGCTCAAA 
TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTGT TGAATTCTTC CAGCTCAGAT 
CTTAGAGGTT TCGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC 
ATTGACGCAG ACGTCAGTGG AAATCAAGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC 
CTACACCTCC TCCTCTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAA 
GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAAGA ACGCTGTCCC 
GTGGTGCTCG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTCATGCAA 
GAAGTAGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CGTGCAGTTT 
GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TGGGAATGAT GGCTCGAACT 
CATTCAGGAT TAACAGATGG CATTCCATTA CAGAGTATTT CTGCTCCGGG CAGTGGGATC 
TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC 
TTGATTGACG TTCTTAAAGA ACTGAATCCA AGTTTGAATT TCAAGGAAGT AACTTATGAA 
CTGGACCATC CTGGATTTCA AATTCGTGAC AGTAAAGGAC TTCATAATGT GGTTTATGGC 
ATTCAGAGGG GTTTGGGTAT GGAAGTGTTC CCAGTAGACC TCATATATAG ACCTTGGAAA 
CATGCTGAAG GCCAGCTCTC CTTCATTCAA CATTCCCTTA TAAATCCAGA GATCTTCTGT 
TTTGCTGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT 
GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTGAGGCTT 
GCAGAGGTTG GGCAGTATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT 
CCAGACATGC TGGTATTGGC CTTACTACAA ATTAACACCT CTTGGCATAC CTTGCGCCAT 
GAACTTATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ATCCTAACTC AGCTATTATT 
TTGCACTATG CATGGCATGG GCAGGGACAG TCTCCCTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTATGATC AGGCCAAATT GTCTCGAATA 
CTTGATGTGG CCCAGGACTT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGATAAGTGG 
CTCACAGATA AAATTCGAGA GCATGGGGAG CCTTTTATCC AGGCGTGTAT GACTTTTTTA 
AAGAGACGGT GTCCTTCTAT TTTGGGCGGA CTTGCCCCAG AAAAAGACCA GCCCAAAAGT 
GCTCAACTTC CTCCAGAAAC TTTGGCGACA ATGTTGGCCT GTCTGCAAGC TTGTGCAGGG 
AGTGTTTCTC AGGAGCTATC AGAAACTATC CTCACCATGG TAGCCAATTG CAGTAATGTT 
ATGAATAAGG CCAGACAACC ACCACCTGGA GTTATGCCAA AAGGACGTCC TCCTAGTGCT 
AGCAGCTTAG ATGCCATTTC TCCTGTTCAG ATTGACCCTC TTGCTGGAAT GACATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCAT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCC 
AATCAGACCA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT 
CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGTGCTC TTGGACTCCC TGCAGTGAAT 
AACGACCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAATCAGCC TACATTCCAG 
CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA CCAGCACTTT 
AGTAAAGAGA TAGATGATGA AGCAAACAGC TATTTCCAGC GAATATATAA TCATCCACCA 
CATCCAACCA TGTCTGTTGA TGAGGTATTA GAAATGCTGC AGAGATTTAA AGACTCTACT 
ATAAAGAGGG AACGAGAAGT ATTTAACTGT ATGCTAAGGA ACTTGTTTGA AGAATATCGT 
TTTTTTCCCC AGTATCCTGA TAAAGAGTTA CATATAACAG CCTGCCTATT TGGTGGTATA 
ATTGAGAAAG GACTGGTCAC TTACATGGCA CTAGGTCTGG CTCTACGATA TGTTCTTGAA 
GCCTTACGCA AGCCTTTTGG ATCCAAAATG TATTATTTCG GGATTGCTGC ACTAGATAGA 
TTTAAAAACA GATTGAAGGA CTATCCCCAG TATTGTCAGC ATTTGGCTTC TATCAGTCAC 
TTTATGCAAT TTCCACATCA TTTACAGGAG TATATTGAGT ATGGACAGCA GTCTAGAGAT 
CCTCCTGTGA AAATGCAAGG CTCTATCACA ACCCCTGGAA GTATTGCACT GGCTCAGGCC 
CAGGCTCAGG CCCAGGTTCC AGCAAAAGCT CCTCTTGCTG GTCAAGTTAG CACTATGGTA 
ACCACCTCAA CAACTACCAC TGTTGCTAAA ACGGTTACGG TCACCAGGCC AACTGGAGTC 
AGCTTTAAGA AAGATGTGCC ACCTTCTATT AATACTACAA ATATAGATAC GTTGCTTGTG 
GCCACAGATC AAACTGAGAG AATTGTGGAG CCCCCAGAAA ATATCCAGGA GAAAATTGCT 
TTTATTTTCA ATAATCTCTC ACAGTCAAAT ATGACACAAA AGGTTGAAGA GCTAAAGGAA 
ACGGTGAAAG AAGAATTTAT GCCTTGGGTT TCACAGTATC TGGTTATGAA GAGAGTCAGT 
ATTGAGCCAA ACTTTCATAG CCTGTATTCA AACTTCCTTG ACACGCTGAA GAATCCTGAA 
TTTAACAAGA TGGTTCTGAA TGAGACCTAC AGAAACATTA AAGTGCTCCT GACCTCTGAT 
AAAGCTGCAG CCAATTTCTC AGATCGTTCT TTGCTGAAGA ACTTGGGACA TTGGCTAGGA 
ATGATCACAT TAGCTAAAAA CAAACCCATC TTACACACTG ACTTGGATGT GAAATCATTG 
CTGCTAGAGG CTTATGTTAA AGGACAACAA GAATTGCTCT ATGTAGTGCC CTTTGTTGCC 
AAAGTCTTAG AATCTAGCAT TCGTAGTGTG GTTTTTAGGC CACCAAACCC TTGGACAATG 
GCAATTATGA ATGTATTAGC TGAGCTACAT CAGGAGCATG ACTTAAAGTT AAACTTGAAG 
TTTGAAATCG AGGTTCTCTG CAAGAACCTT GCATTAGACA TCAATGAGCT AAAACCTGGA 
AACCTCCTAA AGGATAAAGA TCGCCTGAAG AATTTAGATG AGCAACTCTC TGCTCCAAAG 
AAAGATGTCA AGCAGCCAGA AGAACTCCCT CCCATCACAA CCACAACAAC TTCTACTACA 
CCAGCTACCA ACACCACTTG TACAGCCACG GTTCCACCAC AGCCACAGTA CAGCTACCAC 
GACATCAATG TCTATTCCCT TGCGGGCTTG GCACCACACA TTACTCTGAA TCCAACAATT 
CCCTTGTTTC AGGCCCATCC ACAGTTGAAG CAGTGTGTGC GTCAGGCAAT TGAACGGGCT 
GTCCAGGAGC TGGTCCATCC TGTGGTGGAT CGATCAATTA AGATTGCCAT GACTACTTGT 
GAGCAAATAG TCAGGAAGGA TTTTGCCCTG GATTCGGAGG AATCTCGAAT GCGAATAGCA 
GCTCATCACA TGATGCGTAA CTTGACAGCT GGAATGGCTA TGATTACATG CAGGGAACCT 
TTGCTCATGA GCATATCTAC CAACTTAAAA AACAGTTTTG CCTCAGCCCT TCGTACTGCT 
TCCCCACAAC AAAGAGAAAT GATGGATCAG GCAGCTGCTC AATTAGCTCA GGACAATTGT 
GAGTTGGCTT GCTGTTTTAT TCAGAAGACT GCAGTAGAAA AAGCAGGCCC TGAGATGGAC 
AAGAGATTAG CAACTGAATT TGAGCTGAGA AAACATGCTA GGCAAGAAGG ACGCAGATAC 
TGTGATCCTG TTGTTTTAAC ATATCAAGCT GAACGGATGC CAGAGCAAAT CAGGCTGAAA 
GTTGGTGGTG TGGACCCAAA GCAGTTGGCT GTTTACGAAG AGTTTGCACG CAATGTTCCT 
GGCTTCTTAC CTACAAATGA CTTAAGTCAG CCCACGGGAT TTTTAGCCCA GCCCATGAAG 
CAAGCTTGGG CAACAGATGA TGTAGCTCAG ATTTATGATA AGTGTATTAC AGAACTGGAG 
CAACATCTAC ATGCCATCCC ACCAACTTTG GCCATGAACC CTCAAGCTCA GGCTCTTCGA 
AGTCTCTTGG AGGTTGTAGT TTTATCTCGA AACTCTCGGG ATGCCATAGC TGCTCTTGGA 
TTGCTCCAAA AGGCTGTAGA GGGCTTACTA GATGCCACAA GTGGTGCTGA TGCTGACCTT 
CTGCTGCGCT ACAGGGAATG CCACCTCTTG GTCCTAAAAG CTCTGCAGGA TGGCCGGGCA 
TATGGGTCTC CATGGTGCAA CAAACAGATC ACAAGGTGCC TAATTGAATG TCGAGATGAA 
TATAAATATA ATGTGGAGGC TGTGGAGCTG CTAATTCGCA ATCATTTGGT TAATATGCAG 
CAGTATGATC TTCACCTAGC GCAGTCAATG GAGAATGGCT TAAACTACAT GGCTGTGGCA 
TTTGCTATGC AGTTAGTAAA AATCCTGCTG GTGGATGAAA GGAGTGTTGC TCATGTTACT 
GAGGCAGATC TGTTCCACAC CATTGAAACC CTCATGAGGA TTAATGCTCA TTCCAGAGGC 
AATGCTCCAG AAGGATTGCC CCAGCTGATG GAAGTAGTGC GATCCAACTA TGAAGCAATG 
ATTGATCGTG CTCATGGAGG CCCAAACTTT ATGATGCATT CTGGGATCTC TCAAGCCTCA 
GAGTATGATG ACCCTCCAGG CCTGAGGGAG AAGGCAGAGT ATCTTCTGAG GGAATGGGTG 
AATCTCTACC ATTCAGCAGC AGCTGGCCGC GACAGTACCA AAGCTTTCTC TGCATTTGTT 
GGACAGATGC ACCAGCAAGG AATACTGAAG ACCGATGATC TCATAACAAG GTTCTTTCGT 
CTGTGTACTG AAATGTGTGT TGAAATCAGT TACCGTGCTC AGGCTGAGCA GCAGCACAAT 
CCTGCTGCCA ATCCCACCAT GATCCGAGCC AAGTGCTATC ACAACCTGGA TGCCTTTGTT 
CGACTCATTG CACTGCTCGT GAAACACTCA GGGGAGGCCA CCAACACTGT CACAAAGATT 
AATCTGCTGA ACAAGGTCCT TGGTATAGTA GTGGGAGTTC TCCTTCAGGA TCATGATGTT 
CGTCAGAGTG AATTTCAGCA ACTTCCCTAC CATCGAATTT TTATCATGCT TCTCTTGGAA 
CTCAATGCAC CTGAGCATGT GTTGGAAACC ATTAATTTCC AGACACTTAC AGCTTTCTGC 
AATACATTCC ACATCTTGAG GCCTACCAAA GCTCCTGGCT TTGTATATGC CTGGCTTGAA 
CTGATTTCCC ATCGGATATT TATTGCAAGA ATGCTGGCAC ATACGCCACA GCAGAAGGGG 
TGGCCTATGT ATGCACAGCT ACTGATTGAT TTATTCAAAT ATTTAGCGCC TTTCCTTAGA 
AATGTGGAAC TCACCAAACC TATGCAAATC CTCTACAAGG GCACTTTAAG AGTGCTGCTG 
GTTCTTTTGC ATGATTTCCC AGAGTTCCTT TGTGATTACC ATTATGGGTT CTGTGATGTG 
ATCCCACCTA ATTGTATCCA GTTAAGAAAT TTGATCCTGA GTGCCTTTCC AAGAAACATG 
AGGCTCCCCG ACCCATTCAC TCCTAATCTA AAGGTGGACA TGTTGAGTGA AATTAACATT 
GCTCCCCGGA TTCTCACCAA TTTCACTGGA GTAATGCCAC CTCAGTTCAA AAAGGATTTG 
GATTCCTATC TTAAAACTCG ATCACCAGTC ACTTTCCTGT CTGATCTGCG CAGCAACCTA 
CAGGTATCCA ATGAACCTGG GAATCGCTAC AACCTCCAGC TCATCAATGC ACTGGTGCTC 
TATGTCGGGA CTCAGGCCAT TGCGCACATC CACAACAAGG GCAGCACACC TTCAATGAGC 
ACCATCACTC ACTCAGCACA CATGGATATC TTCCAGAATT TGGCTGTGGA CTTGGACACT 
GAGGGTCGCT ATCTCTTTTT GAATGCAATT GCAAATCAGC TCCGGTACCC AAATAGCCAC 
ACTCACTACT TCAGTTGCAC CATGCTGTAC CTTTTTGCAG AGGCCAATAC GGAAGCCATC 
CAAGAACAGA TCACAAGAGT TCTCTTGGAA CGGTTGATTG TAAATAGGCC ACATCCTTGG 
GGTCTTCTTA TTACCTTCAT TGAGCTGATT AAAAACCCAG CGTTTAAGTT CTGGAACCAT 
GAATTTGTAC ACTGTGCCCC AGAAATCGAA AAGTTATTCC AGTCGGTCGC ACAGTGCTGC 
ATGGGACAGA AGCAGGCCCA GCAAGTAATG GAAGGGACAG GTGCCAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( NM_016284.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_057368.3 Homo sapiens CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant 1, mRNA.

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CloneID	OHu08018
Clone ID Related Accession (Same CDS sequence)	NM_016284.4 , NM_016284.5
Accession Version	NM_016284.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	CCR4-NOT transcription complex subunit 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL833549.1, AC010287.9, BX537840.1, AL117492.1, AC009118.11 and BM023419.1. On Nov 23, 2018 this sequence version replaced NM_016284.4. Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.37693.1, SRR1803613.169791.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000317147.10/ ENSP00000320949.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ATTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT 
CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTCCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTTG 
CTGATTACAA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC 
TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTGCTCAAA 
TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTGT TGAATTCTTC CAGCTCAGAT 
CTTAGAGGTT TCGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC 
ATTGACGCAG ACGTCAGTGG AAATCAAGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC 
CTACACCTCC TCCTCTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAA 
GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAAGA ACGCTGTCCC 
GTGGTGCTCG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTCATGCAA 
GAAGTAGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CGTGCAGTTT 
GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TGGGAATGAT GGCTCGAACT 
CATTCAGGAT TAACAGATGG CATTCCATTA CAGAGTATTT CTGCTCCGGG CAGTGGGATC 
TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC 
TTGATTGACG TTCTTAAAGA ACTGAATCCA AGTTTGAATT TCAAGGAAGT AACTTATGAA 
CTGGACCATC CTGGATTTCA AATTCGTGAC AGTAAAGGAC TTCATAATGT GGTTTATGGC 
ATTCAGAGGG GTTTGGGTAT GGAAGTGTTC CCAGTAGACC TCATATATAG ACCTTGGAAA 
CATGCTGAAG GCCAGCTCTC CTTCATTCAA CATTCCCTTA TAAATCCAGA GATCTTCTGT 
TTTGCTGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT 
GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTGAGGCTT 
GCAGAGGTTG GGCAGTATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT 
CCAGACATGC TGGTATTGGC CTTACTACAA ATTAACACCT CTTGGCATAC CTTGCGCCAT 
GAACTTATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ATCCTAACTC AGCTATTATT 
TTGCACTATG CATGGCATGG GCAGGGACAG TCTCCCTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTATGATC AGGCCAAATT GTCTCGAATA 
CTTGATGTGG CCCAGGACTT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGATAAGTGG 
CTCACAGATA AAATTCGAGA GCATGGGGAG CCTTTTATCC AGGCGTGTAT GACTTTTTTA 
AAGAGACGGT GTCCTTCTAT TTTGGGCGGA CTTGCCCCAG AAAAAGACCA GCCCAAAAGT 
GCTCAACTTC CTCCAGAAAC TTTGGCGACA ATGTTGGCCT GTCTGCAAGC TTGTGCAGGG 
AGTGTTTCTC AGGAGCTATC AGAAACTATC CTCACCATGG TAGCCAATTG CAGTAATGTT 
ATGAATAAGG CCAGACAACC ACCACCTGGA GTTATGCCAA AAGGACGTCC TCCTAGTGCT 
AGCAGCTTAG ATGCCATTTC TCCTGTTCAG ATTGACCCTC TTGCTGGAAT GACATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCAT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCC 
AATCAGACCA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT 
CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGTGCTC TTGGACTCCC TGCAGTGAAT 
AACGACCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAATCAGCC TACATTCCAG 
CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA CCAGCACTTT 
AGTAAAGAGA TAGATGATGA AGCAAACAGC TATTTCCAGC GAATATATAA TCATCCACCA 
CATCCAACCA TGTCTGTTGA TGAGGTATTA GAAATGCTGC AGAGATTTAA AGACTCTACT 
ATAAAGAGGG AACGAGAAGT ATTTAACTGT ATGCTAAGGA ACTTGTTTGA AGAATATCGT 
TTTTTTCCCC AGTATCCTGA TAAAGAGTTA CATATAACAG CCTGCCTATT TGGTGGTATA 
ATTGAGAAAG GACTGGTCAC TTACATGGCA CTAGGTCTGG CTCTACGATA TGTTCTTGAA 
GCCTTACGCA AGCCTTTTGG ATCCAAAATG TATTATTTCG GGATTGCTGC ACTAGATAGA 
TTTAAAAACA GATTGAAGGA CTATCCCCAG TATTGTCAGC ATTTGGCTTC TATCAGTCAC 
TTTATGCAAT TTCCACATCA TTTACAGGAG TATATTGAGT ATGGACAGCA GTCTAGAGAT 
CCTCCTGTGA AAATGCAAGG CTCTATCACA ACCCCTGGAA GTATTGCACT GGCTCAGGCC 
CAGGCTCAGG CCCAGGTTCC AGCAAAAGCT CCTCTTGCTG GTCAAGTTAG CACTATGGTA 
ACCACCTCAA CAACTACCAC TGTTGCTAAA ACGGTTACGG TCACCAGGCC AACTGGAGTC 
AGCTTTAAGA AAGATGTGCC ACCTTCTATT AATACTACAA ATATAGATAC GTTGCTTGTG 
GCCACAGATC AAACTGAGAG AATTGTGGAG CCCCCAGAAA ATATCCAGGA GAAAATTGCT 
TTTATTTTCA ATAATCTCTC ACAGTCAAAT ATGACACAAA AGGTTGAAGA GCTAAAGGAA 
ACGGTGAAAG AAGAATTTAT GCCTTGGGTT TCACAGTATC TGGTTATGAA GAGAGTCAGT 
ATTGAGCCAA ACTTTCATAG CCTGTATTCA AACTTCCTTG ACACGCTGAA GAATCCTGAA 
TTTAACAAGA TGGTTCTGAA TGAGACCTAC AGAAACATTA AAGTGCTCCT GACCTCTGAT 
AAAGCTGCAG CCAATTTCTC AGATCGTTCT TTGCTGAAGA ACTTGGGACA TTGGCTAGGA 
ATGATCACAT TAGCTAAAAA CAAACCCATC TTACACACTG ACTTGGATGT GAAATCATTG 
CTGCTAGAGG CTTATGTTAA AGGACAACAA GAATTGCTCT ATGTAGTGCC CTTTGTTGCC 
AAAGTCTTAG AATCTAGCAT TCGTAGTGTG GTTTTTAGGC CACCAAACCC TTGGACAATG 
GCAATTATGA ATGTATTAGC TGAGCTACAT CAGGAGCATG ACTTAAAGTT AAACTTGAAG 
TTTGAAATCG AGGTTCTCTG CAAGAACCTT GCATTAGACA TCAATGAGCT AAAACCTGGA 
AACCTCCTAA AGGATAAAGA TCGCCTGAAG AATTTAGATG AGCAACTCTC TGCTCCAAAG 
AAAGATGTCA AGCAGCCAGA AGAACTCCCT CCCATCACAA CCACAACAAC TTCTACTACA 
CCAGCTACCA ACACCACTTG TACAGCCACG GTTCCACCAC AGCCACAGTA CAGCTACCAC 
GACATCAATG TCTATTCCCT TGCGGGCTTG GCACCACACA TTACTCTGAA TCCAACAATT 
CCCTTGTTTC AGGCCCATCC ACAGTTGAAG CAGTGTGTGC GTCAGGCAAT TGAACGGGCT 
GTCCAGGAGC TGGTCCATCC TGTGGTGGAT CGATCAATTA AGATTGCCAT GACTACTTGT 
GAGCAAATAG TCAGGAAGGA TTTTGCCCTG GATTCGGAGG AATCTCGAAT GCGAATAGCA 
GCTCATCACA TGATGCGTAA CTTGACAGCT GGAATGGCTA TGATTACATG CAGGGAACCT 
TTGCTCATGA GCATATCTAC CAACTTAAAA AACAGTTTTG CCTCAGCCCT TCGTACTGCT 
TCCCCACAAC AAAGAGAAAT GATGGATCAG GCAGCTGCTC AATTAGCTCA GGACAATTGT 
GAGTTGGCTT GCTGTTTTAT TCAGAAGACT GCAGTAGAAA AAGCAGGCCC TGAGATGGAC 
AAGAGATTAG CAACTGAATT TGAGCTGAGA AAACATGCTA GGCAAGAAGG ACGCAGATAC 
TGTGATCCTG TTGTTTTAAC ATATCAAGCT GAACGGATGC CAGAGCAAAT CAGGCTGAAA 
GTTGGTGGTG TGGACCCAAA GCAGTTGGCT GTTTACGAAG AGTTTGCACG CAATGTTCCT 
GGCTTCTTAC CTACAAATGA CTTAAGTCAG CCCACGGGAT TTTTAGCCCA GCCCATGAAG 
CAAGCTTGGG CAACAGATGA TGTAGCTCAG ATTTATGATA AGTGTATTAC AGAACTGGAG 
CAACATCTAC ATGCCATCCC ACCAACTTTG GCCATGAACC CTCAAGCTCA GGCTCTTCGA 
AGTCTCTTGG AGGTTGTAGT TTTATCTCGA AACTCTCGGG ATGCCATAGC TGCTCTTGGA 
TTGCTCCAAA AGGCTGTAGA GGGCTTACTA GATGCCACAA GTGGTGCTGA TGCTGACCTT 
CTGCTGCGCT ACAGGGAATG CCACCTCTTG GTCCTAAAAG CTCTGCAGGA TGGCCGGGCA 
TATGGGTCTC CATGGTGCAA CAAACAGATC ACAAGGTGCC TAATTGAATG TCGAGATGAA 
TATAAATATA ATGTGGAGGC TGTGGAGCTG CTAATTCGCA ATCATTTGGT TAATATGCAG 
CAGTATGATC TTCACCTAGC GCAGTCAATG GAGAATGGCT TAAACTACAT GGCTGTGGCA 
TTTGCTATGC AGTTAGTAAA AATCCTGCTG GTGGATGAAA GGAGTGTTGC TCATGTTACT 
GAGGCAGATC TGTTCCACAC CATTGAAACC CTCATGAGGA TTAATGCTCA TTCCAGAGGC 
AATGCTCCAG AAGGATTGCC CCAGCTGATG GAAGTAGTGC GATCCAACTA TGAAGCAATG 
ATTGATCGTG CTCATGGAGG CCCAAACTTT ATGATGCATT CTGGGATCTC TCAAGCCTCA 
GAGTATGATG ACCCTCCAGG CCTGAGGGAG AAGGCAGAGT ATCTTCTGAG GGAATGGGTG 
AATCTCTACC ATTCAGCAGC AGCTGGCCGC GACAGTACCA AAGCTTTCTC TGCATTTGTT 
GGACAGATGC ACCAGCAAGG AATACTGAAG ACCGATGATC TCATAACAAG GTTCTTTCGT 
CTGTGTACTG AAATGTGTGT TGAAATCAGT TACCGTGCTC AGGCTGAGCA GCAGCACAAT 
CCTGCTGCCA ATCCCACCAT GATCCGAGCC AAGTGCTATC ACAACCTGGA TGCCTTTGTT 
CGACTCATTG CACTGCTCGT GAAACACTCA GGGGAGGCCA CCAACACTGT CACAAAGATT 
AATCTGCTGA ACAAGGTCCT TGGTATAGTA GTGGGAGTTC TCCTTCAGGA TCATGATGTT 
CGTCAGAGTG AATTTCAGCA ACTTCCCTAC CATCGAATTT TTATCATGCT TCTCTTGGAA 
CTCAATGCAC CTGAGCATGT GTTGGAAACC ATTAATTTCC AGACACTTAC AGCTTTCTGC 
AATACATTCC ACATCTTGAG GCCTACCAAA GCTCCTGGCT TTGTATATGC CTGGCTTGAA 
CTGATTTCCC ATCGGATATT TATTGCAAGA ATGCTGGCAC ATACGCCACA GCAGAAGGGG 
TGGCCTATGT ATGCACAGCT ACTGATTGAT TTATTCAAAT ATTTAGCGCC TTTCCTTAGA 
AATGTGGAAC TCACCAAACC TATGCAAATC CTCTACAAGG GCACTTTAAG AGTGCTGCTG 
GTTCTTTTGC ATGATTTCCC AGAGTTCCTT TGTGATTACC ATTATGGGTT CTGTGATGTG 
ATCCCACCTA ATTGTATCCA GTTAAGAAAT TTGATCCTGA GTGCCTTTCC AAGAAACATG 
AGGCTCCCCG ACCCATTCAC TCCTAATCTA AAGGTGGACA TGTTGAGTGA AATTAACATT 
GCTCCCCGGA TTCTCACCAA TTTCACTGGA GTAATGCCAC CTCAGTTCAA AAAGGATTTG 
GATTCCTATC TTAAAACTCG ATCACCAGTC ACTTTCCTGT CTGATCTGCG CAGCAACCTA 
CAGGTATCCA ATGAACCTGG GAATCGCTAC AACCTCCAGC TCATCAATGC ACTGGTGCTC 
TATGTCGGGA CTCAGGCCAT TGCGCACATC CACAACAAGG GCAGCACACC TTCAATGAGC 
ACCATCACTC ACTCAGCACA CATGGATATC TTCCAGAATT TGGCTGTGGA CTTGGACACT 
GAGGGTCGCT ATCTCTTTTT GAATGCAATT GCAAATCAGC TCCGGTACCC AAATAGCCAC 
ACTCACTACT TCAGTTGCAC CATGCTGTAC CTTTTTGCAG AGGCCAATAC GGAAGCCATC 
CAAGAACAGA TCACAAGAGT TCTCTTGGAA CGGTTGATTG TAAATAGGCC ACATCCTTGG 
GGTCTTCTTA TTACCTTCAT TGAGCTGATT AAAAACCCAG CGTTTAAGTT CTGGAACCAT 
GAATTTGTAC ACTGTGCCCC AGAAATCGAA AAGTTATTCC AGTCGGTCGC ACAGTGCTGC 
ATGGGACAGA AGCAGGCCCA GCAAGTAATG GAAGGGACAG GTGCCAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057368.3
CDS	274..7404
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECALLITKPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVGYGFCASIEECRNIIVQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLHNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFSFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDPLAGMTSLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPVGGLGTGSLTGIGTGALGLPAVNNDPFVQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSTIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFGSKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFMQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTMVTTSTTTTVAKTVTVTRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENIQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSIRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDINELKPGNLLKDKDRLKNLDEQLSAPKKDVKQPEELPPITTTTTSTTPATNTTCTATVPPQPQYSYHDINVYSLAGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSISTNLKNSFASALRTASPQQREMMDQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLSQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPTLAMNPQAQALRSLLEVVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERSVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRSNYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQSEFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNLQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	NM_016284.5
Organism	Homo sapiens(human)
Definition	Homo sapiens CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016284.5

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ATTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT 
CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTCCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTTG 
CTGATTACAA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC 
TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTGCTCAAA 
TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTGT TGAATTCTTC CAGCTCAGAT 
CTTAGAGGTT TCGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC 
ATTGACGCAG ACGTCAGTGG AAATCAAGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC 
CTACACCTCC TCCTCTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAA 
GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAAGA ACGCTGTCCC 
GTGGTGCTCG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTCATGCAA 
GAAGTAGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CGTGCAGTTT 
GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TGGGAATGAT GGCTCGAACT 
CATTCAGGAT TAACAGATGG CATTCCATTA CAGAGTATTT CTGCTCCGGG CAGTGGGATC 
TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC 
TTGATTGACG TTCTTAAAGA ACTGAATCCA AGTTTGAATT TCAAGGAAGT AACTTATGAA 
CTGGACCATC CTGGATTTCA AATTCGTGAC AGTAAAGGAC TTCATAATGT GGTTTATGGC 
ATTCAGAGGG GTTTGGGTAT GGAAGTGTTC CCAGTAGACC TCATATATAG ACCTTGGAAA 
CATGCTGAAG GCCAGCTCTC CTTCATTCAA CATTCCCTTA TAAATCCAGA GATCTTCTGT 
TTTGCTGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT 
GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTGAGGCTT 
GCAGAGGTTG GGCAGTATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT 
CCAGACATGC TGGTATTGGC CTTACTACAA ATTAACACCT CTTGGCATAC CTTGCGCCAT 
GAACTTATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ATCCTAACTC AGCTATTATT 
TTGCACTATG CATGGCATGG GCAGGGACAG TCTCCCTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTATGATC AGGCCAAATT GTCTCGAATA 
CTTGATGTGG CCCAGGACTT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGATAAGTGG 
CTCACAGATA AAATTCGAGA GCATGGGGAG CCTTTTATCC AGGCGTGTAT GACTTTTTTA 
AAGAGACGGT GTCCTTCTAT TTTGGGCGGA CTTGCCCCAG AAAAAGACCA GCCCAAAAGT 
GCTCAACTTC CTCCAGAAAC TTTGGCGACA ATGTTGGCCT GTCTGCAAGC TTGTGCAGGG 
AGTGTTTCTC AGGAGCTATC AGAAACTATC CTCACCATGG TAGCCAATTG CAGTAATGTT 
ATGAATAAGG CCAGACAACC ACCACCTGGA GTTATGCCAA AAGGACGTCC TCCTAGTGCT 
AGCAGCTTAG ATGCCATTTC TCCTGTTCAG ATTGACCCTC TTGCTGGAAT GACATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCAT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCC 
AATCAGACCA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT 
CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGTGCTC TTGGACTCCC TGCAGTGAAT 
AACGACCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAATCAGCC TACATTCCAG 
CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA CCAGCACTTT 
AGTAAAGAGA TAGATGATGA AGCAAACAGC TATTTCCAGC GAATATATAA TCATCCACCA 
CATCCAACCA TGTCTGTTGA TGAGGTATTA GAAATGCTGC AGAGATTTAA AGACTCTACT 
ATAAAGAGGG AACGAGAAGT ATTTAACTGT ATGCTAAGGA ACTTGTTTGA AGAATATCGT 
TTTTTTCCCC AGTATCCTGA TAAAGAGTTA CATATAACAG CCTGCCTATT TGGTGGTATA 
ATTGAGAAAG GACTGGTCAC TTACATGGCA CTAGGTCTGG CTCTACGATA TGTTCTTGAA 
GCCTTACGCA AGCCTTTTGG ATCCAAAATG TATTATTTCG GGATTGCTGC ACTAGATAGA 
TTTAAAAACA GATTGAAGGA CTATCCCCAG TATTGTCAGC ATTTGGCTTC TATCAGTCAC 
TTTATGCAAT TTCCACATCA TTTACAGGAG TATATTGAGT ATGGACAGCA GTCTAGAGAT 
CCTCCTGTGA AAATGCAAGG CTCTATCACA ACCCCTGGAA GTATTGCACT GGCTCAGGCC 
CAGGCTCAGG CCCAGGTTCC AGCAAAAGCT CCTCTTGCTG GTCAAGTTAG CACTATGGTA 
ACCACCTCAA CAACTACCAC TGTTGCTAAA ACGGTTACGG TCACCAGGCC AACTGGAGTC 
AGCTTTAAGA AAGATGTGCC ACCTTCTATT AATACTACAA ATATAGATAC GTTGCTTGTG 
GCCACAGATC AAACTGAGAG AATTGTGGAG CCCCCAGAAA ATATCCAGGA GAAAATTGCT 
TTTATTTTCA ATAATCTCTC ACAGTCAAAT ATGACACAAA AGGTTGAAGA GCTAAAGGAA 
ACGGTGAAAG AAGAATTTAT GCCTTGGGTT TCACAGTATC TGGTTATGAA GAGAGTCAGT 
ATTGAGCCAA ACTTTCATAG CCTGTATTCA AACTTCCTTG ACACGCTGAA GAATCCTGAA 
TTTAACAAGA TGGTTCTGAA TGAGACCTAC AGAAACATTA AAGTGCTCCT GACCTCTGAT 
AAAGCTGCAG CCAATTTCTC AGATCGTTCT TTGCTGAAGA ACTTGGGACA TTGGCTAGGA 
ATGATCACAT TAGCTAAAAA CAAACCCATC TTACACACTG ACTTGGATGT GAAATCATTG 
CTGCTAGAGG CTTATGTTAA AGGACAACAA GAATTGCTCT ATGTAGTGCC CTTTGTTGCC 
AAAGTCTTAG AATCTAGCAT TCGTAGTGTG GTTTTTAGGC CACCAAACCC TTGGACAATG 
GCAATTATGA ATGTATTAGC TGAGCTACAT CAGGAGCATG ACTTAAAGTT AAACTTGAAG 
TTTGAAATCG AGGTTCTCTG CAAGAACCTT GCATTAGACA TCAATGAGCT AAAACCTGGA 
AACCTCCTAA AGGATAAAGA TCGCCTGAAG AATTTAGATG AGCAACTCTC TGCTCCAAAG 
AAAGATGTCA AGCAGCCAGA AGAACTCCCT CCCATCACAA CCACAACAAC TTCTACTACA 
CCAGCTACCA ACACCACTTG TACAGCCACG GTTCCACCAC AGCCACAGTA CAGCTACCAC 
GACATCAATG TCTATTCCCT TGCGGGCTTG GCACCACACA TTACTCTGAA TCCAACAATT 
CCCTTGTTTC AGGCCCATCC ACAGTTGAAG CAGTGTGTGC GTCAGGCAAT TGAACGGGCT 
GTCCAGGAGC TGGTCCATCC TGTGGTGGAT CGATCAATTA AGATTGCCAT GACTACTTGT 
GAGCAAATAG TCAGGAAGGA TTTTGCCCTG GATTCGGAGG AATCTCGAAT GCGAATAGCA 
GCTCATCACA TGATGCGTAA CTTGACAGCT GGAATGGCTA TGATTACATG CAGGGAACCT 
TTGCTCATGA GCATATCTAC CAACTTAAAA AACAGTTTTG CCTCAGCCCT TCGTACTGCT 
TCCCCACAAC AAAGAGAAAT GATGGATCAG GCAGCTGCTC AATTAGCTCA GGACAATTGT 
GAGTTGGCTT GCTGTTTTAT TCAGAAGACT GCAGTAGAAA AAGCAGGCCC TGAGATGGAC 
AAGAGATTAG CAACTGAATT TGAGCTGAGA AAACATGCTA GGCAAGAAGG ACGCAGATAC 
TGTGATCCTG TTGTTTTAAC ATATCAAGCT GAACGGATGC CAGAGCAAAT CAGGCTGAAA 
GTTGGTGGTG TGGACCCAAA GCAGTTGGCT GTTTACGAAG AGTTTGCACG CAATGTTCCT 
GGCTTCTTAC CTACAAATGA CTTAAGTCAG CCCACGGGAT TTTTAGCCCA GCCCATGAAG 
CAAGCTTGGG CAACAGATGA TGTAGCTCAG ATTTATGATA AGTGTATTAC AGAACTGGAG 
CAACATCTAC ATGCCATCCC ACCAACTTTG GCCATGAACC CTCAAGCTCA GGCTCTTCGA 
AGTCTCTTGG AGGTTGTAGT TTTATCTCGA AACTCTCGGG ATGCCATAGC TGCTCTTGGA 
TTGCTCCAAA AGGCTGTAGA GGGCTTACTA GATGCCACAA GTGGTGCTGA TGCTGACCTT 
CTGCTGCGCT ACAGGGAATG CCACCTCTTG GTCCTAAAAG CTCTGCAGGA TGGCCGGGCA 
TATGGGTCTC CATGGTGCAA CAAACAGATC ACAAGGTGCC TAATTGAATG TCGAGATGAA 
TATAAATATA ATGTGGAGGC TGTGGAGCTG CTAATTCGCA ATCATTTGGT TAATATGCAG 
CAGTATGATC TTCACCTAGC GCAGTCAATG GAGAATGGCT TAAACTACAT GGCTGTGGCA 
TTTGCTATGC AGTTAGTAAA AATCCTGCTG GTGGATGAAA GGAGTGTTGC TCATGTTACT 
GAGGCAGATC TGTTCCACAC CATTGAAACC CTCATGAGGA TTAATGCTCA TTCCAGAGGC 
AATGCTCCAG AAGGATTGCC CCAGCTGATG GAAGTAGTGC GATCCAACTA TGAAGCAATG 
ATTGATCGTG CTCATGGAGG CCCAAACTTT ATGATGCATT CTGGGATCTC TCAAGCCTCA 
GAGTATGATG ACCCTCCAGG CCTGAGGGAG AAGGCAGAGT ATCTTCTGAG GGAATGGGTG 
AATCTCTACC ATTCAGCAGC AGCTGGCCGC GACAGTACCA AAGCTTTCTC TGCATTTGTT 
GGACAGATGC ACCAGCAAGG AATACTGAAG ACCGATGATC TCATAACAAG GTTCTTTCGT 
CTGTGTACTG AAATGTGTGT TGAAATCAGT TACCGTGCTC AGGCTGAGCA GCAGCACAAT 
CCTGCTGCCA ATCCCACCAT GATCCGAGCC AAGTGCTATC ACAACCTGGA TGCCTTTGTT 
CGACTCATTG CACTGCTCGT GAAACACTCA GGGGAGGCCA CCAACACTGT CACAAAGATT 
AATCTGCTGA ACAAGGTCCT TGGTATAGTA GTGGGAGTTC TCCTTCAGGA TCATGATGTT 
CGTCAGAGTG AATTTCAGCA ACTTCCCTAC CATCGAATTT TTATCATGCT TCTCTTGGAA 
CTCAATGCAC CTGAGCATGT GTTGGAAACC ATTAATTTCC AGACACTTAC AGCTTTCTGC 
AATACATTCC ACATCTTGAG GCCTACCAAA GCTCCTGGCT TTGTATATGC CTGGCTTGAA 
CTGATTTCCC ATCGGATATT TATTGCAAGA ATGCTGGCAC ATACGCCACA GCAGAAGGGG 
TGGCCTATGT ATGCACAGCT ACTGATTGAT TTATTCAAAT ATTTAGCGCC TTTCCTTAGA 
AATGTGGAAC TCACCAAACC TATGCAAATC CTCTACAAGG GCACTTTAAG AGTGCTGCTG 
GTTCTTTTGC ATGATTTCCC AGAGTTCCTT TGTGATTACC ATTATGGGTT CTGTGATGTG 
ATCCCACCTA ATTGTATCCA GTTAAGAAAT TTGATCCTGA GTGCCTTTCC AAGAAACATG 
AGGCTCCCCG ACCCATTCAC TCCTAATCTA AAGGTGGACA TGTTGAGTGA AATTAACATT 
GCTCCCCGGA TTCTCACCAA TTTCACTGGA GTAATGCCAC CTCAGTTCAA AAAGGATTTG 
GATTCCTATC TTAAAACTCG ATCACCAGTC ACTTTCCTGT CTGATCTGCG CAGCAACCTA 
CAGGTATCCA ATGAACCTGG GAATCGCTAC AACCTCCAGC TCATCAATGC ACTGGTGCTC 
TATGTCGGGA CTCAGGCCAT TGCGCACATC CACAACAAGG GCAGCACACC TTCAATGAGC 
ACCATCACTC ACTCAGCACA CATGGATATC TTCCAGAATT TGGCTGTGGA CTTGGACACT 
GAGGGTCGCT ATCTCTTTTT GAATGCAATT GCAAATCAGC TCCGGTACCC AAATAGCCAC 
ACTCACTACT TCAGTTGCAC CATGCTGTAC CTTTTTGCAG AGGCCAATAC GGAAGCCATC 
CAAGAACAGA TCACAAGAGT TCTCTTGGAA CGGTTGATTG TAAATAGGCC ACATCCTTGG 
GGTCTTCTTA TTACCTTCAT TGAGCTGATT AAAAACCCAG CGTTTAAGTT CTGGAACCAT 
GAATTTGTAC ACTGTGCCCC AGAAATCGAA AAGTTATTCC AGTCGGTCGC ACAGTGCTGC 
ATGGGACAGA AGCAGGCCCA GCAAGTAATG GAAGGGACAG GTGCCAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( NM_001265612.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001252541.1 Homo sapiens CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant 3, mRNA.

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CloneID	OHu08102
Clone ID Related Accession (Same CDS sequence)	NM_001265612.2 , NM_001265612.1
Accession Version	NM_001265612.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-18
Organism	Homo sapiens(human)
Product	CCR4-NOT transcription complex subunit 1 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EF553522.1, BC040523.1, AC009118.11 and BM023419.1. On May 31, 2019 this sequence version replaced NM_001265612.1. Transcript Variant: This variant (3) uses an alternate splice site in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.281971.1, SRR1803614.16474.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ATTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT 
CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTCCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTTG 
CTGATTACAA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC 
TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTGCTCAAA 
TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTGT TGAATTCTTC CAGCTCAGAT 
CTTAGAGGTT TCGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC 
ATTGACGCAG ACGTCAGTGG AAATCAAGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC 
CTACACCTCC TCCTCTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAA 
GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAAGA ACGCTGTCCC 
GTGGTGCTCG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTCATGCAA 
GAAGTAGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CGTGCAGTTT 
GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TGGGAATGAT GGCTCGAACT 
CATTCAGGAT TAACAGATGG CATTCCATTA CAGAGTATTT CTGCTCCGGG CAGTGGGATC 
TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC 
TTGATTGACG TTCTTAAAGA ACTGAATCCA AGTTTGAATT TCAAGGAAGT AACTTATGAA 
CTGGACCATC CTGGATTTCA AATTCGTGAC AGTAAAGGAC TTCATAATGT GGTTTATGGC 
ATTCAGAGGG GTTTGGGTAT GGAAGTGTTC CCAGTAGACC TCATATATAG ACCTTGGAAA 
CATGCTGAAG GCCAGCTCTC CTTCATTCAA CATTCCCTTA TAAATCCAGA GATCTTCTGT 
TTTGCTGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT 
GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTGAGGCTT 
GCAGAGGTTG GGCAGTATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT 
CCAGACATGC TGGTATTGGC CTTACTACAA ATTAACACCT CTTGGCATAC CTTGCGCCAT 
GAACTTATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ATCCTAACTC AGCTATTATT 
TTGCACTATG CATGGCATGG GCAGGGACAG TCTCCCTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTATGATC AGGCCAAATT GTCTCGAATA 
CTTGATGTGG CCCAGGACTT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGATAAGTGG 
CTCACAGATA AAATTCGAGA GCATGGGGAG CCTTTTATCC AGGCGTGTAT GACTTTTTTA 
AAGAGACGGT GTCCTTCTAT TTTGGGCGGA CTTGCCCCAG AAAAAGACCA GCCCAAAAGT 
GCTCAACTTC CTCCAGAAAC TTTGGCGACA ATGTTGGCCT GTCTGCAAGC TTGTGCAGGG 
AGTGTTTCTC AGGAGCTATC AGAAACTATC CTCACCATGG TAGCCAATTG CAGTAATGTT 
ATGAATAAGG CCAGACAACC ACCACCTGGA GTTATGCCAA AAGGACGTCC TCCTAGTGCT 
AGCAGCTTAG ATGCCATTTC TCCTGTTCAG ATTGACCCTC TTGCTGGAAT GACATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCAT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCC 
AATCAGACCA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT 
CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGTGCTC TTGGACTCCC TGCAGTGAAT 
AACGACCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAATCAGCC TACATTCCAG 
CAGACGGACT TGTCTCAGGT GTGGCCAGAG GCAAACCAGC ACTTTAGTAA AGAGATAGAT 
GATGAAGCAA ACAGCTATTT CCAGCGAATA TATAATCATC CACCACATCC AACCATGTCT 
GTTGATGAGG TATTAGAAAT GCTGCAGAGA TTTAAAGACT CTACTATAAA GAGGGAACGA 
GAAGTATTTA ACTGTATGCT AAGGAACTTG TTTGAAGAAT ATCGTTTTTT TCCCCAGTAT 
CCTGATAAAG AGTTACATAT AACAGCCTGC CTATTTGGTG GTATAATTGA GAAAGGACTG 
GTCACTTACA TGGCACTAGG TCTGGCTCTA CGATATGTTC TTGAAGCCTT ACGCAAGCCT 
TTTGGATCCA AAATGTATTA TTTCGGGATT GCTGCACTAG ATAGATTTAA AAACAGATTG 
AAGGACTATC CCCAGTATTG TCAGCATTTG GCTTCTATCA GTCACTTTAT GCAATTTCCA 
CATCATTTAC AGGAGTATAT TGAGTATGGA CAGCAGTCTA GAGATCCTCC TGTGAAAATG 
CAAGGCTCTA TCACAACCCC TGGAAGTATT GCACTGGCTC AGGCCCAGGC TCAGGCCCAG 
GTTCCAGCAA AAGCTCCTCT TGCTGGTCAA GTTAGCACTA TGGTAACCAC CTCAACAACT 
ACCACTGTTG CTAAAACGGT TACGGTCACC AGGCCAACTG GAGTCAGCTT TAAGAAAGAT 
GTGCCACCTT CTATTAATAC TACAAATATA GATACGTTGC TTGTGGCCAC AGATCAAACT 
GAGAGAATTG TGGAGCCCCC AGAAAATATC CAGGAGAAAA TTGCTTTTAT TTTCAATAAT 
CTCTCACAGT CAAATATGAC ACAAAAGGTT GAAGAGCTAA AGGAAACGGT GAAAGAAGAA 
TTTATGCCTT GGGTTTCACA GTATCTGGTT ATGAAGAGAG TCAGTATTGA GCCAAACTTT 
CATAGCCTGT ATTCAAACTT CCTTGACACG CTGAAGAATC CTGAATTTAA CAAGATGGTT 
CTGAATGAGA CCTACAGAAA CATTAAAGTG CTCCTGACCT CTGATAAAGC TGCAGCCAAT 
TTCTCAGATC GTTCTTTGCT GAAGAACTTG GGACATTGGC TAGGAATGAT CACATTAGCT 
AAAAACAAAC CCATCTTACA CACTGACTTG GATGTGAAAT CATTGCTGCT AGAGGCTTAT 
GTTAAAGGAC AACAAGAATT GCTCTATGTA GTGCCCTTTG TTGCCAAAGT CTTAGAATCT 
AGCATTCGTA GTGTGGTTTT TAGGCCACCA AACCCTTGGA CAATGGCAAT TATGAATGTA 
TTAGCTGAGC TACATCAGGA GCATGACTTA AAGTTAAACT TGAAGTTTGA AATCGAGGTT 
CTCTGCAAGA ACCTTGCATT AGACATCAAT GAGCTAAAAC CTGGAAACCT CCTAAAGGAT 
AAAGATCGCC TGAAGAATTT AGATGAGCAA CTCTCTGCTC CAAAGAAAGA TGTCAAGCAG 
CCAGAAGAAC TCCCTCCCAT CACAACCACA ACAACTTCTA CTACACCAGC TACCAACACC 
ACTTGTACAG CCACGGTTCC ACCACAGCCA CAGTACAGCT ACCACGACAT CAATGTCTAT 
TCCCTTGCGG GCTTGGCACC ACACATTACT CTGAATCCAA CAATTCCCTT GTTTCAGGCC 
CATCCACAGT TGAAGCAGTG TGTGCGTCAG GCAATTGAAC GGGCTGTCCA GGAGCTGGTC 
CATCCTGTGG TGGATCGATC AATTAAGATT GCCATGACTA CTTGTGAGCA AATAGTCAGG 
AAGGATTTTG CCCTGGATTC GGAGGAATCT CGAATGCGAA TAGCAGCTCA TCACATGATG 
CGTAACTTGA CAGCTGGAAT GGCTATGATT ACATGCAGGG AACCTTTGCT CATGAGCATA 
TCTACCAACT TAAAAAACAG TTTTGCCTCA GCCCTTCGTA CTGCTTCCCC ACAACAAAGA 
GAAATGATGG ATCAGGCAGC TGCTCAATTA GCTCAGGACA ATTGTGAGTT GGCTTGCTGT 
TTTATTCAGA AGACTGCAGT AGAAAAAGCA GGCCCTGAGA TGGACAAGAG ATTAGCAACT 
GAATTTGAGC TGAGAAAACA TGCTAGGCAA GAAGGACGCA GATACTGTGA TCCTGTTGTT 
TTAACATATC AAGCTGAACG GATGCCAGAG CAAATCAGGC TGAAAGTTGG TGGTGTGGAC 
CCAAAGCAGT TGGCTGTTTA CGAAGAGTTT GCACGCAATG TTCCTGGCTT CTTACCTACA 
AATGACTTAA GTCAGCCCAC GGGATTTTTA GCCCAGCCCA TGAAGCAAGC TTGGGCAACA 
GATGATGTAG CTCAGATTTA TGATAAGTGT ATTACAGAAC TGGAGCAACA TCTACATGCC 
ATCCCACCAA CTTTGGCCAT GAACCCTCAA GCTCAGGCTC TTCGAAGTCT CTTGGAGGTT 
GTAGTTTTAT CTCGAAACTC TCGGGATGCC ATAGCTGCTC TTGGATTGCT CCAAAAGGCT 
GTAGAGGGCT TACTAGATGC CACAAGTGGT GCTGATGCTG ACCTTCTGCT GCGCTACAGG 
GAATGCCACC TCTTGGTCCT AAAAGCTCTG CAGGATGGCC GGGCATATGG GTCTCCATGG 
TGCAACAAAC AGATCACAAG GTGCCTAATT GAATGTCGAG ATGAATATAA ATATAATGTG 
GAGGCTGTGG AGCTGCTAAT TCGCAATCAT TTGGTTAATA TGCAGCAGTA TGATCTTCAC 
CTAGCGCAGT CAATGGAGAA TGGCTTAAAC TACATGGCTG TGGCATTTGC TATGCAGTTA 
GTAAAAATCC TGCTGGTGGA TGAAAGGAGT GTTGCTCATG TTACTGAGGC AGATCTGTTC 
CACACCATTG AAACCCTCAT GAGGATTAAT GCTCATTCCA GAGGCAATGC TCCAGAAGGA 
TTGCCCCAGC TGATGGAAGT AGTGCGATCC AACTATGAAG CAATGATTGA TCGTGCTCAT 
GGAGGCCCAA ACTTTATGAT GCATTCTGGG ATCTCTCAAG CCTCAGAGTA TGATGACCCT 
CCAGGCCTGA GGGAGAAGGC AGAGTATCTT CTGAGGGAAT GGGTGAATCT CTACCATTCA 
GCAGCAGCTG GCCGCGACAG TACCAAAGCT TTCTCTGCAT TTGTTGGACA GATGCACCAG 
CAAGGAATAC TGAAGACCGA TGATCTCATA ACAAGGTTCT TTCGTCTGTG TACTGAAATG 
TGTGTTGAAA TCAGTTACCG TGCTCAGGCT GAGCAGCAGC ACAATCCTGC TGCCAATCCC 
ACCATGATCC GAGCCAAGTG CTATCACAAC CTGGATGCCT TTGTTCGACT CATTGCACTG 
CTCGTGAAAC ACTCAGGGGA GGCCACCAAC ACTGTCACAA AGATTAATCT GCTGAACAAG 
GTCCTTGGTA TAGTAGTGGG AGTTCTCCTT CAGGATCATG ATGTTCGTCA GAGTGAATTT 
CAGCAACTTC CCTACCATCG AATTTTTATC ATGCTTCTCT TGGAACTCAA TGCACCTGAG 
CATGTGTTGG AAACCATTAA TTTCCAGACA CTTACAGCTT TCTGCAATAC ATTCCACATC 
TTGAGGCCTA CCAAAGCTCC TGGCTTTGTA TATGCCTGGC TTGAACTGAT TTCCCATCGG 
ATATTTATTG CAAGAATGCT GGCACATACG CCACAGCAGA AGGGGTGGCC TATGTATGCA 
CAGCTACTGA TTGATTTATT CAAATATTTA GCGCCTTTCC TTAGAAATGT GGAACTCACC 
AAACCTATGC AAATCCTCTA CAAGGGCACT TTAAGAGTGC TGCTGGTTCT TTTGCATGAT 
TTCCCAGAGT TCCTTTGTGA TTACCATTAT GGGTTCTGTG ATGTGATCCC ACCTAATTGT 
ATCCAGTTAA GAAATTTGAT CCTGAGTGCC TTTCCAAGAA ACATGAGGCT CCCCGACCCA 
TTCACTCCTA ATCTAAAGGT GGACATGTTG AGTGAAATTA ACATTGCTCC CCGGATTCTC 
ACCAATTTCA CTGGAGTAAT GCCACCTCAG TTCAAAAAGG ATTTGGATTC CTATCTTAAA 
ACTCGATCAC CAGTCACTTT CCTGTCTGAT CTGCGCAGCA ACCTACAGGT ATCCAATGAA 
CCTGGGAATC GCTACAACCT CCAGCTCATC AATGCACTGG TGCTCTATGT CGGGACTCAG 
GCCATTGCGC ACATCCACAA CAAGGGCAGC ACACCTTCAA TGAGCACCAT CACTCACTCA 
GCACACATGG ATATCTTCCA GAATTTGGCT GTGGACTTGG ACACTGAGGG TCGCTATCTC 
TTTTTGAATG CAATTGCAAA TCAGCTCCGG TACCCAAATA GCCACACTCA CTACTTCAGT 
TGCACCATGC TGTACCTTTT TGCAGAGGCC AATACGGAAG CCATCCAAGA ACAGATCACA 
AGAGTTCTCT TGGAACGGTT GATTGTAAAT AGGCCACATC CTTGGGGTCT TCTTATTACC 
TTCATTGAGC TGATTAAAAA CCCAGCGTTT AAGTTCTGGA ACCATGAATT TGTACACTGT 
GCCCCAGAAA TCGAAAAGTT ATTCCAGTCG GTCGCACAGT GCTGCATGGG ACAGAAGCAG 
GCCCAGCAAG TAATGGAAGG GACAGGTGCC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001252541.1
CDS	274..7389
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECALLITKPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVGYGFCASIEECRNIIVQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLHNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFSFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDPLAGMTSLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPVGGLGTGSLTGIGTGALGLPAVNNDPFVQRKLGTSGLNQPTFQQTDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSTIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFGSKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFMQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTMVTTSTTTTVAKTVTVTRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENIQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSIRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDINELKPGNLLKDKDRLKNLDEQLSAPKKDVKQPEELPPITTTTTSTTPATNTTCTATVPPQPQYSYHDINVYSLAGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSISTNLKNSFASALRTASPQQREMMDQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLSQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPTLAMNPQAQALRSLLEVVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERSVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRSNYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQSEFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNLQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	NM_001265612.2
Organism	Homo sapiens(human)
Definition	Homo sapiens CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001265612.2

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ATTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT 
CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTCCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTTG 
CTGATTACAA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC 
TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTGCTCAAA 
TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTGT TGAATTCTTC CAGCTCAGAT 
CTTAGAGGTT TCGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC 
ATTGACGCAG ACGTCAGTGG AAATCAAGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC 
CTACACCTCC TCCTCTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAA 
GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAAGA ACGCTGTCCC 
GTGGTGCTCG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTCATGCAA 
GAAGTAGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CGTGCAGTTT 
GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TGGGAATGAT GGCTCGAACT 
CATTCAGGAT TAACAGATGG CATTCCATTA CAGAGTATTT CTGCTCCGGG CAGTGGGATC 
TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC 
TTGATTGACG TTCTTAAAGA ACTGAATCCA AGTTTGAATT TCAAGGAAGT AACTTATGAA 
CTGGACCATC CTGGATTTCA AATTCGTGAC AGTAAAGGAC TTCATAATGT GGTTTATGGC 
ATTCAGAGGG GTTTGGGTAT GGAAGTGTTC CCAGTAGACC TCATATATAG ACCTTGGAAA 
CATGCTGAAG GCCAGCTCTC CTTCATTCAA CATTCCCTTA TAAATCCAGA GATCTTCTGT 
TTTGCTGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT 
GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTGAGGCTT 
GCAGAGGTTG GGCAGTATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT 
CCAGACATGC TGGTATTGGC CTTACTACAA ATTAACACCT CTTGGCATAC CTTGCGCCAT 
GAACTTATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ATCCTAACTC AGCTATTATT 
TTGCACTATG CATGGCATGG GCAGGGACAG TCTCCCTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTATGATC AGGCCAAATT GTCTCGAATA 
CTTGATGTGG CCCAGGACTT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGATAAGTGG 
CTCACAGATA AAATTCGAGA GCATGGGGAG CCTTTTATCC AGGCGTGTAT GACTTTTTTA 
AAGAGACGGT GTCCTTCTAT TTTGGGCGGA CTTGCCCCAG AAAAAGACCA GCCCAAAAGT 
GCTCAACTTC CTCCAGAAAC TTTGGCGACA ATGTTGGCCT GTCTGCAAGC TTGTGCAGGG 
AGTGTTTCTC AGGAGCTATC AGAAACTATC CTCACCATGG TAGCCAATTG CAGTAATGTT 
ATGAATAAGG CCAGACAACC ACCACCTGGA GTTATGCCAA AAGGACGTCC TCCTAGTGCT 
AGCAGCTTAG ATGCCATTTC TCCTGTTCAG ATTGACCCTC TTGCTGGAAT GACATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCAT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCC 
AATCAGACCA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT 
CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGTGCTC TTGGACTCCC TGCAGTGAAT 
AACGACCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAATCAGCC TACATTCCAG 
CAGACGGACT TGTCTCAGGT GTGGCCAGAG GCAAACCAGC ACTTTAGTAA AGAGATAGAT 
GATGAAGCAA ACAGCTATTT CCAGCGAATA TATAATCATC CACCACATCC AACCATGTCT 
GTTGATGAGG TATTAGAAAT GCTGCAGAGA TTTAAAGACT CTACTATAAA GAGGGAACGA 
GAAGTATTTA ACTGTATGCT AAGGAACTTG TTTGAAGAAT ATCGTTTTTT TCCCCAGTAT 
CCTGATAAAG AGTTACATAT AACAGCCTGC CTATTTGGTG GTATAATTGA GAAAGGACTG 
GTCACTTACA TGGCACTAGG TCTGGCTCTA CGATATGTTC TTGAAGCCTT ACGCAAGCCT 
TTTGGATCCA AAATGTATTA TTTCGGGATT GCTGCACTAG ATAGATTTAA AAACAGATTG 
AAGGACTATC CCCAGTATTG TCAGCATTTG GCTTCTATCA GTCACTTTAT GCAATTTCCA 
CATCATTTAC AGGAGTATAT TGAGTATGGA CAGCAGTCTA GAGATCCTCC TGTGAAAATG 
CAAGGCTCTA TCACAACCCC TGGAAGTATT GCACTGGCTC AGGCCCAGGC TCAGGCCCAG 
GTTCCAGCAA AAGCTCCTCT TGCTGGTCAA GTTAGCACTA TGGTAACCAC CTCAACAACT 
ACCACTGTTG CTAAAACGGT TACGGTCACC AGGCCAACTG GAGTCAGCTT TAAGAAAGAT 
GTGCCACCTT CTATTAATAC TACAAATATA GATACGTTGC TTGTGGCCAC AGATCAAACT 
GAGAGAATTG TGGAGCCCCC AGAAAATATC CAGGAGAAAA TTGCTTTTAT TTTCAATAAT 
CTCTCACAGT CAAATATGAC ACAAAAGGTT GAAGAGCTAA AGGAAACGGT GAAAGAAGAA 
TTTATGCCTT GGGTTTCACA GTATCTGGTT ATGAAGAGAG TCAGTATTGA GCCAAACTTT 
CATAGCCTGT ATTCAAACTT CCTTGACACG CTGAAGAATC CTGAATTTAA CAAGATGGTT 
CTGAATGAGA CCTACAGAAA CATTAAAGTG CTCCTGACCT CTGATAAAGC TGCAGCCAAT 
TTCTCAGATC GTTCTTTGCT GAAGAACTTG GGACATTGGC TAGGAATGAT CACATTAGCT 
AAAAACAAAC CCATCTTACA CACTGACTTG GATGTGAAAT CATTGCTGCT AGAGGCTTAT 
GTTAAAGGAC AACAAGAATT GCTCTATGTA GTGCCCTTTG TTGCCAAAGT CTTAGAATCT 
AGCATTCGTA GTGTGGTTTT TAGGCCACCA AACCCTTGGA CAATGGCAAT TATGAATGTA 
TTAGCTGAGC TACATCAGGA GCATGACTTA AAGTTAAACT TGAAGTTTGA AATCGAGGTT 
CTCTGCAAGA ACCTTGCATT AGACATCAAT GAGCTAAAAC CTGGAAACCT CCTAAAGGAT 
AAAGATCGCC TGAAGAATTT AGATGAGCAA CTCTCTGCTC CAAAGAAAGA TGTCAAGCAG 
CCAGAAGAAC TCCCTCCCAT CACAACCACA ACAACTTCTA CTACACCAGC TACCAACACC 
ACTTGTACAG CCACGGTTCC ACCACAGCCA CAGTACAGCT ACCACGACAT CAATGTCTAT 
TCCCTTGCGG GCTTGGCACC ACACATTACT CTGAATCCAA CAATTCCCTT GTTTCAGGCC 
CATCCACAGT TGAAGCAGTG TGTGCGTCAG GCAATTGAAC GGGCTGTCCA GGAGCTGGTC 
CATCCTGTGG TGGATCGATC AATTAAGATT GCCATGACTA CTTGTGAGCA AATAGTCAGG 
AAGGATTTTG CCCTGGATTC GGAGGAATCT CGAATGCGAA TAGCAGCTCA TCACATGATG 
CGTAACTTGA CAGCTGGAAT GGCTATGATT ACATGCAGGG AACCTTTGCT CATGAGCATA 
TCTACCAACT TAAAAAACAG TTTTGCCTCA GCCCTTCGTA CTGCTTCCCC ACAACAAAGA 
GAAATGATGG ATCAGGCAGC TGCTCAATTA GCTCAGGACA ATTGTGAGTT GGCTTGCTGT 
TTTATTCAGA AGACTGCAGT AGAAAAAGCA GGCCCTGAGA TGGACAAGAG ATTAGCAACT 
GAATTTGAGC TGAGAAAACA TGCTAGGCAA GAAGGACGCA GATACTGTGA TCCTGTTGTT 
TTAACATATC AAGCTGAACG GATGCCAGAG CAAATCAGGC TGAAAGTTGG TGGTGTGGAC 
CCAAAGCAGT TGGCTGTTTA CGAAGAGTTT GCACGCAATG TTCCTGGCTT CTTACCTACA 
AATGACTTAA GTCAGCCCAC GGGATTTTTA GCCCAGCCCA TGAAGCAAGC TTGGGCAACA 
GATGATGTAG CTCAGATTTA TGATAAGTGT ATTACAGAAC TGGAGCAACA TCTACATGCC 
ATCCCACCAA CTTTGGCCAT GAACCCTCAA GCTCAGGCTC TTCGAAGTCT CTTGGAGGTT 
GTAGTTTTAT CTCGAAACTC TCGGGATGCC ATAGCTGCTC TTGGATTGCT CCAAAAGGCT 
GTAGAGGGCT TACTAGATGC CACAAGTGGT GCTGATGCTG ACCTTCTGCT GCGCTACAGG 
GAATGCCACC TCTTGGTCCT AAAAGCTCTG CAGGATGGCC GGGCATATGG GTCTCCATGG 
TGCAACAAAC AGATCACAAG GTGCCTAATT GAATGTCGAG ATGAATATAA ATATAATGTG 
GAGGCTGTGG AGCTGCTAAT TCGCAATCAT TTGGTTAATA TGCAGCAGTA TGATCTTCAC 
CTAGCGCAGT CAATGGAGAA TGGCTTAAAC TACATGGCTG TGGCATTTGC TATGCAGTTA 
GTAAAAATCC TGCTGGTGGA TGAAAGGAGT GTTGCTCATG TTACTGAGGC AGATCTGTTC 
CACACCATTG AAACCCTCAT GAGGATTAAT GCTCATTCCA GAGGCAATGC TCCAGAAGGA 
TTGCCCCAGC TGATGGAAGT AGTGCGATCC AACTATGAAG CAATGATTGA TCGTGCTCAT 
GGAGGCCCAA ACTTTATGAT GCATTCTGGG ATCTCTCAAG CCTCAGAGTA TGATGACCCT 
CCAGGCCTGA GGGAGAAGGC AGAGTATCTT CTGAGGGAAT GGGTGAATCT CTACCATTCA 
GCAGCAGCTG GCCGCGACAG TACCAAAGCT TTCTCTGCAT TTGTTGGACA GATGCACCAG 
CAAGGAATAC TGAAGACCGA TGATCTCATA ACAAGGTTCT TTCGTCTGTG TACTGAAATG 
TGTGTTGAAA TCAGTTACCG TGCTCAGGCT GAGCAGCAGC ACAATCCTGC TGCCAATCCC 
ACCATGATCC GAGCCAAGTG CTATCACAAC CTGGATGCCT TTGTTCGACT CATTGCACTG 
CTCGTGAAAC ACTCAGGGGA GGCCACCAAC ACTGTCACAA AGATTAATCT GCTGAACAAG 
GTCCTTGGTA TAGTAGTGGG AGTTCTCCTT CAGGATCATG ATGTTCGTCA GAGTGAATTT 
CAGCAACTTC CCTACCATCG AATTTTTATC ATGCTTCTCT TGGAACTCAA TGCACCTGAG 
CATGTGTTGG AAACCATTAA TTTCCAGACA CTTACAGCTT TCTGCAATAC ATTCCACATC 
TTGAGGCCTA CCAAAGCTCC TGGCTTTGTA TATGCCTGGC TTGAACTGAT TTCCCATCGG 
ATATTTATTG CAAGAATGCT GGCACATACG CCACAGCAGA AGGGGTGGCC TATGTATGCA 
CAGCTACTGA TTGATTTATT CAAATATTTA GCGCCTTTCC TTAGAAATGT GGAACTCACC 
AAACCTATGC AAATCCTCTA CAAGGGCACT TTAAGAGTGC TGCTGGTTCT TTTGCATGAT 
TTCCCAGAGT TCCTTTGTGA TTACCATTAT GGGTTCTGTG ATGTGATCCC ACCTAATTGT 
ATCCAGTTAA GAAATTTGAT CCTGAGTGCC TTTCCAAGAA ACATGAGGCT CCCCGACCCA 
TTCACTCCTA ATCTAAAGGT GGACATGTTG AGTGAAATTA ACATTGCTCC CCGGATTCTC 
ACCAATTTCA CTGGAGTAAT GCCACCTCAG TTCAAAAAGG ATTTGGATTC CTATCTTAAA 
ACTCGATCAC CAGTCACTTT CCTGTCTGAT CTGCGCAGCA ACCTACAGGT ATCCAATGAA 
CCTGGGAATC GCTACAACCT CCAGCTCATC AATGCACTGG TGCTCTATGT CGGGACTCAG 
GCCATTGCGC ACATCCACAA CAAGGGCAGC ACACCTTCAA TGAGCACCAT CACTCACTCA 
GCACACATGG ATATCTTCCA GAATTTGGCT GTGGACTTGG ACACTGAGGG TCGCTATCTC 
TTTTTGAATG CAATTGCAAA TCAGCTCCGG TACCCAAATA GCCACACTCA CTACTTCAGT 
TGCACCATGC TGTACCTTTT TGCAGAGGCC AATACGGAAG CCATCCAAGA ACAGATCACA 
AGAGTTCTCT TGGAACGGTT GATTGTAAAT AGGCCACATC CTTGGGGTCT TCTTATTACC 
TTCATTGAGC TGATTAAAAA CCCAGCGTTT AAGTTCTGGA ACCATGAATT TGTACACTGT 
GCCCCAGAAA TCGAAAAGTT ATTCCAGTCG GTCGCACAGT GCTGCATGGG ACAGAAGCAG 
GCCCAGCAAG TAATGGAAGG GACAGGTGCC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( NM_001265612.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001252541.1 Homo sapiens CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant 3, mRNA.

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CloneID	OHu08102
Clone ID Related Accession (Same CDS sequence)	NM_001265612.2 , NM_001265612.1
Accession Version	NM_001265612.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	CCR4-NOT transcription complex subunit 1 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB456343.1, EF553522.1, BC040523.1, AC009118.11 and BM023419.1. Transcript Variant: This variant (3) uses an alternate splice site in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.16474.1, SRR1803614.281971.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ATTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT 
CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTCCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTTG 
CTGATTACAA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC 
TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTGCTCAAA 
TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTGT TGAATTCTTC CAGCTCAGAT 
CTTAGAGGTT TCGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC 
ATTGACGCAG ACGTCAGTGG AAATCAAGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC 
CTACACCTCC TCCTCTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAA 
GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAAGA ACGCTGTCCC 
GTGGTGCTCG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTCATGCAA 
GAAGTAGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CGTGCAGTTT 
GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TGGGAATGAT GGCTCGAACT 
CATTCAGGAT TAACAGATGG CATTCCATTA CAGAGTATTT CTGCTCCGGG CAGTGGGATC 
TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC 
TTGATTGACG TTCTTAAAGA ACTGAATCCA AGTTTGAATT TCAAGGAAGT AACTTATGAA 
CTGGACCATC CTGGATTTCA AATTCGTGAC AGTAAAGGAC TTCATAATGT GGTTTATGGC 
ATTCAGAGGG GTTTGGGTAT GGAAGTGTTC CCAGTAGACC TCATATATAG ACCTTGGAAA 
CATGCTGAAG GCCAGCTCTC CTTCATTCAA CATTCCCTTA TAAATCCAGA GATCTTCTGT 
TTTGCTGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT 
GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTGAGGCTT 
GCAGAGGTTG GGCAGTATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT 
CCAGACATGC TGGTATTGGC CTTACTACAA ATTAACACCT CTTGGCATAC CTTGCGCCAT 
GAACTTATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ATCCTAACTC AGCTATTATT 
TTGCACTATG CATGGCATGG GCAGGGACAG TCTCCCTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTATGATC AGGCCAAATT GTCTCGAATA 
CTTGATGTGG CCCAGGACTT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGATAAGTGG 
CTCACAGATA AAATTCGAGA GCATGGGGAG CCTTTTATCC AGGCGTGTAT GACTTTTTTA 
AAGAGACGGT GTCCTTCTAT TTTGGGCGGA CTTGCCCCAG AAAAAGACCA GCCCAAAAGT 
GCTCAACTTC CTCCAGAAAC TTTGGCGACA ATGTTGGCCT GTCTGCAAGC TTGTGCAGGG 
AGTGTTTCTC AGGAGCTATC AGAAACTATC CTCACCATGG TAGCCAATTG CAGTAATGTT 
ATGAATAAGG CCAGACAACC ACCACCTGGA GTTATGCCAA AAGGACGTCC TCCTAGTGCT 
AGCAGCTTAG ATGCCATTTC TCCTGTTCAG ATTGACCCTC TTGCTGGAAT GACATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCAT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCC 
AATCAGACCA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT 
CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGTGCTC TTGGACTCCC TGCAGTGAAT 
AACGACCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAATCAGCC TACATTCCAG 
CAGACGGACT TGTCTCAGGT GTGGCCAGAG GCAAACCAGC ACTTTAGTAA AGAGATAGAT 
GATGAAGCAA ACAGCTATTT CCAGCGAATA TATAATCATC CACCACATCC AACCATGTCT 
GTTGATGAGG TATTAGAAAT GCTGCAGAGA TTTAAAGACT CTACTATAAA GAGGGAACGA 
GAAGTATTTA ACTGTATGCT AAGGAACTTG TTTGAAGAAT ATCGTTTTTT TCCCCAGTAT 
CCTGATAAAG AGTTACATAT AACAGCCTGC CTATTTGGTG GTATAATTGA GAAAGGACTG 
GTCACTTACA TGGCACTAGG TCTGGCTCTA CGATATGTTC TTGAAGCCTT ACGCAAGCCT 
TTTGGATCCA AAATGTATTA TTTCGGGATT GCTGCACTAG ATAGATTTAA AAACAGATTG 
AAGGACTATC CCCAGTATTG TCAGCATTTG GCTTCTATCA GTCACTTTAT GCAATTTCCA 
CATCATTTAC AGGAGTATAT TGAGTATGGA CAGCAGTCTA GAGATCCTCC TGTGAAAATG 
CAAGGCTCTA TCACAACCCC TGGAAGTATT GCACTGGCTC AGGCCCAGGC TCAGGCCCAG 
GTTCCAGCAA AAGCTCCTCT TGCTGGTCAA GTTAGCACTA TGGTAACCAC CTCAACAACT 
ACCACTGTTG CTAAAACGGT TACGGTCACC AGGCCAACTG GAGTCAGCTT TAAGAAAGAT 
GTGCCACCTT CTATTAATAC TACAAATATA GATACGTTGC TTGTGGCCAC AGATCAAACT 
GAGAGAATTG TGGAGCCCCC AGAAAATATC CAGGAGAAAA TTGCTTTTAT TTTCAATAAT 
CTCTCACAGT CAAATATGAC ACAAAAGGTT GAAGAGCTAA AGGAAACGGT GAAAGAAGAA 
TTTATGCCTT GGGTTTCACA GTATCTGGTT ATGAAGAGAG TCAGTATTGA GCCAAACTTT 
CATAGCCTGT ATTCAAACTT CCTTGACACG CTGAAGAATC CTGAATTTAA CAAGATGGTT 
CTGAATGAGA CCTACAGAAA CATTAAAGTG CTCCTGACCT CTGATAAAGC TGCAGCCAAT 
TTCTCAGATC GTTCTTTGCT GAAGAACTTG GGACATTGGC TAGGAATGAT CACATTAGCT 
AAAAACAAAC CCATCTTACA CACTGACTTG GATGTGAAAT CATTGCTGCT AGAGGCTTAT 
GTTAAAGGAC AACAAGAATT GCTCTATGTA GTGCCCTTTG TTGCCAAAGT CTTAGAATCT 
AGCATTCGTA GTGTGGTTTT TAGGCCACCA AACCCTTGGA CAATGGCAAT TATGAATGTA 
TTAGCTGAGC TACATCAGGA GCATGACTTA AAGTTAAACT TGAAGTTTGA AATCGAGGTT 
CTCTGCAAGA ACCTTGCATT AGACATCAAT GAGCTAAAAC CTGGAAACCT CCTAAAGGAT 
AAAGATCGCC TGAAGAATTT AGATGAGCAA CTCTCTGCTC CAAAGAAAGA TGTCAAGCAG 
CCAGAAGAAC TCCCTCCCAT CACAACCACA ACAACTTCTA CTACACCAGC TACCAACACC 
ACTTGTACAG CCACGGTTCC ACCACAGCCA CAGTACAGCT ACCACGACAT CAATGTCTAT 
TCCCTTGCGG GCTTGGCACC ACACATTACT CTGAATCCAA CAATTCCCTT GTTTCAGGCC 
CATCCACAGT TGAAGCAGTG TGTGCGTCAG GCAATTGAAC GGGCTGTCCA GGAGCTGGTC 
CATCCTGTGG TGGATCGATC AATTAAGATT GCCATGACTA CTTGTGAGCA AATAGTCAGG 
AAGGATTTTG CCCTGGATTC GGAGGAATCT CGAATGCGAA TAGCAGCTCA TCACATGATG 
CGTAACTTGA CAGCTGGAAT GGCTATGATT ACATGCAGGG AACCTTTGCT CATGAGCATA 
TCTACCAACT TAAAAAACAG TTTTGCCTCA GCCCTTCGTA CTGCTTCCCC ACAACAAAGA 
GAAATGATGG ATCAGGCAGC TGCTCAATTA GCTCAGGACA ATTGTGAGTT GGCTTGCTGT 
TTTATTCAGA AGACTGCAGT AGAAAAAGCA GGCCCTGAGA TGGACAAGAG ATTAGCAACT 
GAATTTGAGC TGAGAAAACA TGCTAGGCAA GAAGGACGCA GATACTGTGA TCCTGTTGTT 
TTAACATATC AAGCTGAACG GATGCCAGAG CAAATCAGGC TGAAAGTTGG TGGTGTGGAC 
CCAAAGCAGT TGGCTGTTTA CGAAGAGTTT GCACGCAATG TTCCTGGCTT CTTACCTACA 
AATGACTTAA GTCAGCCCAC GGGATTTTTA GCCCAGCCCA TGAAGCAAGC TTGGGCAACA 
GATGATGTAG CTCAGATTTA TGATAAGTGT ATTACAGAAC TGGAGCAACA TCTACATGCC 
ATCCCACCAA CTTTGGCCAT GAACCCTCAA GCTCAGGCTC TTCGAAGTCT CTTGGAGGTT 
GTAGTTTTAT CTCGAAACTC TCGGGATGCC ATAGCTGCTC TTGGATTGCT CCAAAAGGCT 
GTAGAGGGCT TACTAGATGC CACAAGTGGT GCTGATGCTG ACCTTCTGCT GCGCTACAGG 
GAATGCCACC TCTTGGTCCT AAAAGCTCTG CAGGATGGCC GGGCATATGG GTCTCCATGG 
TGCAACAAAC AGATCACAAG GTGCCTAATT GAATGTCGAG ATGAATATAA ATATAATGTG 
GAGGCTGTGG AGCTGCTAAT TCGCAATCAT TTGGTTAATA TGCAGCAGTA TGATCTTCAC 
CTAGCGCAGT CAATGGAGAA TGGCTTAAAC TACATGGCTG TGGCATTTGC TATGCAGTTA 
GTAAAAATCC TGCTGGTGGA TGAAAGGAGT GTTGCTCATG TTACTGAGGC AGATCTGTTC 
CACACCATTG AAACCCTCAT GAGGATTAAT GCTCATTCCA GAGGCAATGC TCCAGAAGGA 
TTGCCCCAGC TGATGGAAGT AGTGCGATCC AACTATGAAG CAATGATTGA TCGTGCTCAT 
GGAGGCCCAA ACTTTATGAT GCATTCTGGG ATCTCTCAAG CCTCAGAGTA TGATGACCCT 
CCAGGCCTGA GGGAGAAGGC AGAGTATCTT CTGAGGGAAT GGGTGAATCT CTACCATTCA 
GCAGCAGCTG GCCGCGACAG TACCAAAGCT TTCTCTGCAT TTGTTGGACA GATGCACCAG 
CAAGGAATAC TGAAGACCGA TGATCTCATA ACAAGGTTCT TTCGTCTGTG TACTGAAATG 
TGTGTTGAAA TCAGTTACCG TGCTCAGGCT GAGCAGCAGC ACAATCCTGC TGCCAATCCC 
ACCATGATCC GAGCCAAGTG CTATCACAAC CTGGATGCCT TTGTTCGACT CATTGCACTG 
CTCGTGAAAC ACTCAGGGGA GGCCACCAAC ACTGTCACAA AGATTAATCT GCTGAACAAG 
GTCCTTGGTA TAGTAGTGGG AGTTCTCCTT CAGGATCATG ATGTTCGTCA GAGTGAATTT 
CAGCAACTTC CCTACCATCG AATTTTTATC ATGCTTCTCT TGGAACTCAA TGCACCTGAG 
CATGTGTTGG AAACCATTAA TTTCCAGACA CTTACAGCTT TCTGCAATAC ATTCCACATC 
TTGAGGCCTA CCAAAGCTCC TGGCTTTGTA TATGCCTGGC TTGAACTGAT TTCCCATCGG 
ATATTTATTG CAAGAATGCT GGCACATACG CCACAGCAGA AGGGGTGGCC TATGTATGCA 
CAGCTACTGA TTGATTTATT CAAATATTTA GCGCCTTTCC TTAGAAATGT GGAACTCACC 
AAACCTATGC AAATCCTCTA CAAGGGCACT TTAAGAGTGC TGCTGGTTCT TTTGCATGAT 
TTCCCAGAGT TCCTTTGTGA TTACCATTAT GGGTTCTGTG ATGTGATCCC ACCTAATTGT 
ATCCAGTTAA GAAATTTGAT CCTGAGTGCC TTTCCAAGAA ACATGAGGCT CCCCGACCCA 
TTCACTCCTA ATCTAAAGGT GGACATGTTG AGTGAAATTA ACATTGCTCC CCGGATTCTC 
ACCAATTTCA CTGGAGTAAT GCCACCTCAG TTCAAAAAGG ATTTGGATTC CTATCTTAAA 
ACTCGATCAC CAGTCACTTT CCTGTCTGAT CTGCGCAGCA ACCTACAGGT ATCCAATGAA 
CCTGGGAATC GCTACAACCT CCAGCTCATC AATGCACTGG TGCTCTATGT CGGGACTCAG 
GCCATTGCGC ACATCCACAA CAAGGGCAGC ACACCTTCAA TGAGCACCAT CACTCACTCA 
GCACACATGG ATATCTTCCA GAATTTGGCT GTGGACTTGG ACACTGAGGG TCGCTATCTC 
TTTTTGAATG CAATTGCAAA TCAGCTCCGG TACCCAAATA GCCACACTCA CTACTTCAGT 
TGCACCATGC TGTACCTTTT TGCAGAGGCC AATACGGAAG CCATCCAAGA ACAGATCACA 
AGAGTTCTCT TGGAACGGTT GATTGTAAAT AGGCCACATC CTTGGGGTCT TCTTATTACC 
TTCATTGAGC TGATTAAAAA CCCAGCGTTT AAGTTCTGGA ACCATGAATT TGTACACTGT 
GCCCCAGAAA TCGAAAAGTT ATTCCAGTCG GTCGCACAGT GCTGCATGGG ACAGAAGCAG 
GCCCAGCAAG TAATGGAAGG GACAGGTGCC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001252541.1
CDS	334..7449
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECALLITKPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVGYGFCASIEECRNIIVQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLHNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFSFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDPLAGMTSLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPVGGLGTGSLTGIGTGALGLPAVNNDPFVQRKLGTSGLNQPTFQQTDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSTIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFGSKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFMQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTMVTTSTTTTVAKTVTVTRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENIQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSIRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDINELKPGNLLKDKDRLKNLDEQLSAPKKDVKQPEELPPITTTTTSTTPATNTTCTATVPPQPQYSYHDINVYSLAGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSISTNLKNSFASALRTASPQQREMMDQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLSQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPTLAMNPQAQALRSLLEVVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERSVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRSNYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQSEFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNLQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	NM_001265612.1
Organism	Homo sapiens(human)
Definition	Homo sapiens CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001265612.1

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ATTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT 
CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTCCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTTG 
CTGATTACAA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC 
TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTGCTCAAA 
TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTGT TGAATTCTTC CAGCTCAGAT 
CTTAGAGGTT TCGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC 
ATTGACGCAG ACGTCAGTGG AAATCAAGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC 
CTACACCTCC TCCTCTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAA 
GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAAGA ACGCTGTCCC 
GTGGTGCTCG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTCATGCAA 
GAAGTAGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CGTGCAGTTT 
GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TGGGAATGAT GGCTCGAACT 
CATTCAGGAT TAACAGATGG CATTCCATTA CAGAGTATTT CTGCTCCGGG CAGTGGGATC 
TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC 
TTGATTGACG TTCTTAAAGA ACTGAATCCA AGTTTGAATT TCAAGGAAGT AACTTATGAA 
CTGGACCATC CTGGATTTCA AATTCGTGAC AGTAAAGGAC TTCATAATGT GGTTTATGGC 
ATTCAGAGGG GTTTGGGTAT GGAAGTGTTC CCAGTAGACC TCATATATAG ACCTTGGAAA 
CATGCTGAAG GCCAGCTCTC CTTCATTCAA CATTCCCTTA TAAATCCAGA GATCTTCTGT 
TTTGCTGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT 
GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTGAGGCTT 
GCAGAGGTTG GGCAGTATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT 
CCAGACATGC TGGTATTGGC CTTACTACAA ATTAACACCT CTTGGCATAC CTTGCGCCAT 
GAACTTATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ATCCTAACTC AGCTATTATT 
TTGCACTATG CATGGCATGG GCAGGGACAG TCTCCCTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTATGATC AGGCCAAATT GTCTCGAATA 
CTTGATGTGG CCCAGGACTT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGATAAGTGG 
CTCACAGATA AAATTCGAGA GCATGGGGAG CCTTTTATCC AGGCGTGTAT GACTTTTTTA 
AAGAGACGGT GTCCTTCTAT TTTGGGCGGA CTTGCCCCAG AAAAAGACCA GCCCAAAAGT 
GCTCAACTTC CTCCAGAAAC TTTGGCGACA ATGTTGGCCT GTCTGCAAGC TTGTGCAGGG 
AGTGTTTCTC AGGAGCTATC AGAAACTATC CTCACCATGG TAGCCAATTG CAGTAATGTT 
ATGAATAAGG CCAGACAACC ACCACCTGGA GTTATGCCAA AAGGACGTCC TCCTAGTGCT 
AGCAGCTTAG ATGCCATTTC TCCTGTTCAG ATTGACCCTC TTGCTGGAAT GACATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCAT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCC 
AATCAGACCA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT 
CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGTGCTC TTGGACTCCC TGCAGTGAAT 
AACGACCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAATCAGCC TACATTCCAG 
CAGACGGACT TGTCTCAGGT GTGGCCAGAG GCAAACCAGC ACTTTAGTAA AGAGATAGAT 
GATGAAGCAA ACAGCTATTT CCAGCGAATA TATAATCATC CACCACATCC AACCATGTCT 
GTTGATGAGG TATTAGAAAT GCTGCAGAGA TTTAAAGACT CTACTATAAA GAGGGAACGA 
GAAGTATTTA ACTGTATGCT AAGGAACTTG TTTGAAGAAT ATCGTTTTTT TCCCCAGTAT 
CCTGATAAAG AGTTACATAT AACAGCCTGC CTATTTGGTG GTATAATTGA GAAAGGACTG 
GTCACTTACA TGGCACTAGG TCTGGCTCTA CGATATGTTC TTGAAGCCTT ACGCAAGCCT 
TTTGGATCCA AAATGTATTA TTTCGGGATT GCTGCACTAG ATAGATTTAA AAACAGATTG 
AAGGACTATC CCCAGTATTG TCAGCATTTG GCTTCTATCA GTCACTTTAT GCAATTTCCA 
CATCATTTAC AGGAGTATAT TGAGTATGGA CAGCAGTCTA GAGATCCTCC TGTGAAAATG 
CAAGGCTCTA TCACAACCCC TGGAAGTATT GCACTGGCTC AGGCCCAGGC TCAGGCCCAG 
GTTCCAGCAA AAGCTCCTCT TGCTGGTCAA GTTAGCACTA TGGTAACCAC CTCAACAACT 
ACCACTGTTG CTAAAACGGT TACGGTCACC AGGCCAACTG GAGTCAGCTT TAAGAAAGAT 
GTGCCACCTT CTATTAATAC TACAAATATA GATACGTTGC TTGTGGCCAC AGATCAAACT 
GAGAGAATTG TGGAGCCCCC AGAAAATATC CAGGAGAAAA TTGCTTTTAT TTTCAATAAT 
CTCTCACAGT CAAATATGAC ACAAAAGGTT GAAGAGCTAA AGGAAACGGT GAAAGAAGAA 
TTTATGCCTT GGGTTTCACA GTATCTGGTT ATGAAGAGAG TCAGTATTGA GCCAAACTTT 
CATAGCCTGT ATTCAAACTT CCTTGACACG CTGAAGAATC CTGAATTTAA CAAGATGGTT 
CTGAATGAGA CCTACAGAAA CATTAAAGTG CTCCTGACCT CTGATAAAGC TGCAGCCAAT 
TTCTCAGATC GTTCTTTGCT GAAGAACTTG GGACATTGGC TAGGAATGAT CACATTAGCT 
AAAAACAAAC CCATCTTACA CACTGACTTG GATGTGAAAT CATTGCTGCT AGAGGCTTAT 
GTTAAAGGAC AACAAGAATT GCTCTATGTA GTGCCCTTTG TTGCCAAAGT CTTAGAATCT 
AGCATTCGTA GTGTGGTTTT TAGGCCACCA AACCCTTGGA CAATGGCAAT TATGAATGTA 
TTAGCTGAGC TACATCAGGA GCATGACTTA AAGTTAAACT TGAAGTTTGA AATCGAGGTT 
CTCTGCAAGA ACCTTGCATT AGACATCAAT GAGCTAAAAC CTGGAAACCT CCTAAAGGAT 
AAAGATCGCC TGAAGAATTT AGATGAGCAA CTCTCTGCTC CAAAGAAAGA TGTCAAGCAG 
CCAGAAGAAC TCCCTCCCAT CACAACCACA ACAACTTCTA CTACACCAGC TACCAACACC 
ACTTGTACAG CCACGGTTCC ACCACAGCCA CAGTACAGCT ACCACGACAT CAATGTCTAT 
TCCCTTGCGG GCTTGGCACC ACACATTACT CTGAATCCAA CAATTCCCTT GTTTCAGGCC 
CATCCACAGT TGAAGCAGTG TGTGCGTCAG GCAATTGAAC GGGCTGTCCA GGAGCTGGTC 
CATCCTGTGG TGGATCGATC AATTAAGATT GCCATGACTA CTTGTGAGCA AATAGTCAGG 
AAGGATTTTG CCCTGGATTC GGAGGAATCT CGAATGCGAA TAGCAGCTCA TCACATGATG 
CGTAACTTGA CAGCTGGAAT GGCTATGATT ACATGCAGGG AACCTTTGCT CATGAGCATA 
TCTACCAACT TAAAAAACAG TTTTGCCTCA GCCCTTCGTA CTGCTTCCCC ACAACAAAGA 
GAAATGATGG ATCAGGCAGC TGCTCAATTA GCTCAGGACA ATTGTGAGTT GGCTTGCTGT 
TTTATTCAGA AGACTGCAGT AGAAAAAGCA GGCCCTGAGA TGGACAAGAG ATTAGCAACT 
GAATTTGAGC TGAGAAAACA TGCTAGGCAA GAAGGACGCA GATACTGTGA TCCTGTTGTT 
TTAACATATC AAGCTGAACG GATGCCAGAG CAAATCAGGC TGAAAGTTGG TGGTGTGGAC 
CCAAAGCAGT TGGCTGTTTA CGAAGAGTTT GCACGCAATG TTCCTGGCTT CTTACCTACA 
AATGACTTAA GTCAGCCCAC GGGATTTTTA GCCCAGCCCA TGAAGCAAGC TTGGGCAACA 
GATGATGTAG CTCAGATTTA TGATAAGTGT ATTACAGAAC TGGAGCAACA TCTACATGCC 
ATCCCACCAA CTTTGGCCAT GAACCCTCAA GCTCAGGCTC TTCGAAGTCT CTTGGAGGTT 
GTAGTTTTAT CTCGAAACTC TCGGGATGCC ATAGCTGCTC TTGGATTGCT CCAAAAGGCT 
GTAGAGGGCT TACTAGATGC CACAAGTGGT GCTGATGCTG ACCTTCTGCT GCGCTACAGG 
GAATGCCACC TCTTGGTCCT AAAAGCTCTG CAGGATGGCC GGGCATATGG GTCTCCATGG 
TGCAACAAAC AGATCACAAG GTGCCTAATT GAATGTCGAG ATGAATATAA ATATAATGTG 
GAGGCTGTGG AGCTGCTAAT TCGCAATCAT TTGGTTAATA TGCAGCAGTA TGATCTTCAC 
CTAGCGCAGT CAATGGAGAA TGGCTTAAAC TACATGGCTG TGGCATTTGC TATGCAGTTA 
GTAAAAATCC TGCTGGTGGA TGAAAGGAGT GTTGCTCATG TTACTGAGGC AGATCTGTTC 
CACACCATTG AAACCCTCAT GAGGATTAAT GCTCATTCCA GAGGCAATGC TCCAGAAGGA 
TTGCCCCAGC TGATGGAAGT AGTGCGATCC AACTATGAAG CAATGATTGA TCGTGCTCAT 
GGAGGCCCAA ACTTTATGAT GCATTCTGGG ATCTCTCAAG CCTCAGAGTA TGATGACCCT 
CCAGGCCTGA GGGAGAAGGC AGAGTATCTT CTGAGGGAAT GGGTGAATCT CTACCATTCA 
GCAGCAGCTG GCCGCGACAG TACCAAAGCT TTCTCTGCAT TTGTTGGACA GATGCACCAG 
CAAGGAATAC TGAAGACCGA TGATCTCATA ACAAGGTTCT TTCGTCTGTG TACTGAAATG 
TGTGTTGAAA TCAGTTACCG TGCTCAGGCT GAGCAGCAGC ACAATCCTGC TGCCAATCCC 
ACCATGATCC GAGCCAAGTG CTATCACAAC CTGGATGCCT TTGTTCGACT CATTGCACTG 
CTCGTGAAAC ACTCAGGGGA GGCCACCAAC ACTGTCACAA AGATTAATCT GCTGAACAAG 
GTCCTTGGTA TAGTAGTGGG AGTTCTCCTT CAGGATCATG ATGTTCGTCA GAGTGAATTT 
CAGCAACTTC CCTACCATCG AATTTTTATC ATGCTTCTCT TGGAACTCAA TGCACCTGAG 
CATGTGTTGG AAACCATTAA TTTCCAGACA CTTACAGCTT TCTGCAATAC ATTCCACATC 
TTGAGGCCTA CCAAAGCTCC TGGCTTTGTA TATGCCTGGC TTGAACTGAT TTCCCATCGG 
ATATTTATTG CAAGAATGCT GGCACATACG CCACAGCAGA AGGGGTGGCC TATGTATGCA 
CAGCTACTGA TTGATTTATT CAAATATTTA GCGCCTTTCC TTAGAAATGT GGAACTCACC 
AAACCTATGC AAATCCTCTA CAAGGGCACT TTAAGAGTGC TGCTGGTTCT TTTGCATGAT 
TTCCCAGAGT TCCTTTGTGA TTACCATTAT GGGTTCTGTG ATGTGATCCC ACCTAATTGT 
ATCCAGTTAA GAAATTTGAT CCTGAGTGCC TTTCCAAGAA ACATGAGGCT CCCCGACCCA 
TTCACTCCTA ATCTAAAGGT GGACATGTTG AGTGAAATTA ACATTGCTCC CCGGATTCTC 
ACCAATTTCA CTGGAGTAAT GCCACCTCAG TTCAAAAAGG ATTTGGATTC CTATCTTAAA 
ACTCGATCAC CAGTCACTTT CCTGTCTGAT CTGCGCAGCA ACCTACAGGT ATCCAATGAA 
CCTGGGAATC GCTACAACCT CCAGCTCATC AATGCACTGG TGCTCTATGT CGGGACTCAG 
GCCATTGCGC ACATCCACAA CAAGGGCAGC ACACCTTCAA TGAGCACCAT CACTCACTCA 
GCACACATGG ATATCTTCCA GAATTTGGCT GTGGACTTGG ACACTGAGGG TCGCTATCTC 
TTTTTGAATG CAATTGCAAA TCAGCTCCGG TACCCAAATA GCCACACTCA CTACTTCAGT 
TGCACCATGC TGTACCTTTT TGCAGAGGCC AATACGGAAG CCATCCAAGA ACAGATCACA 
AGAGTTCTCT TGGAACGGTT GATTGTAAAT AGGCCACATC CTTGGGGTCT TCTTATTACC 
TTCATTGAGC TGATTAAAAA CCCAGCGTTT AAGTTCTGGA ACCATGAATT TGTACACTGT 
GCCCCAGAAA TCGAAAAGTT ATTCCAGTCG GTCGCACAGT GCTGCATGGG ACAGAAGCAG 
GCCCAGCAAG TAATGGAAGG GACAGGTGCC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The CCR4-NOT complex contributes to repression of Major Histocompatibility Complex class II transcription.
Scientific reports7(1)3547(2017 Jun)
Rodr?guez-Gil A,Ritter O,Saul VV,Wilhelm J,Yang CY,Grosschedl R,Imai Y,Kuba K,Kracht M,Schmitz ML

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CNOT1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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