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FLI1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol FLI1
Entrez Gene ID 2313
Full Name Fli-1 proto-oncogene, ETS transcription factor
Synonyms EWSR2, SIC-1
General protein information
Preferred Names
Friend leukemia integration 1 transcription factor
Names
Friend leukemia integration 1 transcription factor
proto-oncogene Fli-1
transcription factor ERGB
Ewing sarcoma breakpoint region 2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11q24.1-q24.3

Summary This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]. lac of sum
Disorder MIM:

193067


mRNA and Protein(s)

mRNA Protein Name
XM_011542701 XP_011541003 Friend leukemia integration 1 transcription factor isoform X1
XM_011542702 XP_011541004 Friend leukemia integration 1 transcription factor isoform X2
NM_002017 NP_002008 Friend leukemia integration 1 transcription factor isoform 1
NM_001271010 NP_001257939 Friend leukemia integration 1 transcription factor isoform 3
NM_001271012 NP_001257941 Friend leukemia integration 1 transcription factor isoform 4
NM_001167681 NP_001161153 Friend leukemia integration 1 transcription factor isoform 2



Homo sapiens (human) FLI1 NP_002008.2
Pan troglodytes (chimpanzee) FLI1 XP_508856.3
Macaca mulatta (Rhesus monkey) FLI1 XP_002799924.1
Canis lupus familiaris (dog) FLI1 XP_546404.2
Bos taurus (cattle) FLI1 NP_001039763.1
Mus musculus (house mouse) Fli1 NP_032052.1
Rattus norvegicus (Norway rat) Fli1 NP_001017381.1
Gallus gallus (chicken) FLI1 NP_001026079.1
Danio rerio (zebrafish) fli1a NP_571423.1
Xenopus (Silurana) tropicalis (western clawed frog) fli1 NP_001096189.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following FLI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FLI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu20678
XM_011542701 PREDICTED: Homo sapiens Fli-1 proto-oncogene, ETS transcription factor (FLI1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu57800 XM_011542702 PREDICTED: Homo sapiens Fli-1 proto-oncogene, ETS transcription factor (FLI1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu20383
NM_002017 Homo sapiens Fli-1 proto-oncogene, ETS transcription factor (FLI1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu20623 NM_001271010 Homo sapiens Fli-1 proto-oncogene, ETS transcription factor (FLI1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu21072 NM_001271012 Homo sapiens Fli-1 proto-oncogene, ETS transcription factor (FLI1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu20678
NM_001167681 Homo sapiens Fli-1 proto-oncogene, ETS transcription factor (FLI1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu20678
Clone ID Related Accession (Same CDS sequence) NM_001167681 , XM_011542701
Accession Version XM_011542701.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1260bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product Friend leukemia integration 1 transcription factor isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_033899.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGACTGCCT CGGGGAGTCC TGACTACGGG CAGCCCCACA AGATCAACCC CCTCCCACCA 
CAGCAGGAGT GGATCAATCA GCCAGTGAGG GTCAACGTCA AGCGGGAGTA TGACCACATG
AATGGATCCA GGGAGTCTCC GGTGGACTGC AGCGTTAGCA AATGCAGCAA GCTGGTGGGC
GGAGGCGAGT CCAACCCCAT GAACTACAAC AGCTATATGG ACGAGAAGAA TGGCCCCCCT
CCTCCCAACA TGACCACCAA CGAGAGGAGA GTCATCGTCC CCGCAGACCC CACACTGTGG
ACACAGGAGC ATGTGAGGCA ATGGCTGGAG TGGGCCATAA AGGAGTACAG CTTGATGGAG
ATCGACACAT CCTTTTTCCA GAACATGGAT GGCAAGGAAC TGTGTAAAAT GAACAAGGAG
GACTTCCTCC GCGCCACCAC CCTCTACAAC ACGGAAGTGC TGTTGTCACA CCTCAGTTAC
CTCAGGGAAA GTTCACTGCT GGCCTATAAT ACAACCTCCC ACACCGACCA ATCCTCACGA
TTGAGTGTCA AAGAAGACCC TTCTTATGAC TCAGTCAGAA GAGGAGCTTG GGGCAATAAC
ATGAATTCTG GCCTCAACAA AAGTCCTCCC CTTGGAGGGG CACAAACGAT CAGTAAGAAT
ACAGAGCAAC GGCCCCAGCC AGATCCGTAT CAGATCCTGG GCCCGACCAG CAGTCGCCTA
GCCAACCCTG GAAGCGGGCA GATCCAGCTG TGGCAATTCC TCCTGGAGCT GCTCTCCGAC
AGCGCCAACG CCAGCTGTAT CACCTGGGAG GGGACCAACG GGGAGTTCAA AATGACGGAC
CCCGATGAGG TGGCCAGGCG CTGGGGCGAG CGGAAAAGCA AGCCCAACAT GAATTACGAC
AAGCTGAGCC GGGCCCTCCG TTATTACTAT GATAAAAACA TTATGACCAA AGTGCACGGC
AAAAGATATG CTTACAAATT TGACTTCCAC GGCATTGCCC AGGCTCTGCA GCCACATCCG
ACCGAGTCGT CCATGTACAA GTACCCTTCT GACATCTCCT ACATGCCTTC CTACCATGCC
CACCAGCAGA AGGTGAACTT TGTCCCTCCC CATCCATCCT CCATGCCTGT CACTTCCTCC
AGCTTCTTTG GAGCCGCATC ACAATACTGG ACCTCCCCCA CGGGGGGAAT CTACCCCAAC
CCCAACGTCC CCCGCCATCC TAACACCCAC GTGCCTTCAC ACTTAGGCAG CTACTACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011541003.1
CDS159..1418
Misc Feature(1)399..671(+)
Misc Feature(2)897..1148(+)
Translation

Target ORF information:

RefSeq Version XM_011542701
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Fli-1 proto-oncogene, ETS transcription factor (FLI1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011542701

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGACTGCCT CGGGGAGTCC TGACTACGGG CAGCCCCACA AGATCAACCC CCTCCCACCA 
CAGCAGGAGT GGATCAATCA GCCAGTGAGG GTCAACGTCA AGCGGGAGTA TGACCACATG
AATGGATCCA GGGAGTCTCC GGTGGACTGC AGCGTTAGCA AATGCAGCAA GCTGGTGGGC
GGAGGCGAGT CCAACCCCAT GAACTACAAC AGCTATATGG ACGAGAAGAA TGGCCCCCCT
CCTCCCAACA TGACCACCAA CGAGAGGAGA GTCATCGTCC CCGCAGACCC CACACTGTGG
ACACAGGAGC ATGTGAGGCA ATGGCTGGAG TGGGCCATAA AGGAGTACAG CTTGATGGAG
ATCGACACAT CCTTTTTCCA GAACATGGAT GGCAAGGAAC TGTGTAAAAT GAACAAGGAG
GACTTCCTCC GCGCCACCAC CCTCTACAAC ACGGAAGTGC TGTTGTCACA CCTCAGTTAC
CTCAGGGAAA GTTCACTGCT GGCCTATAAT ACAACCTCCC ACACCGACCA ATCCTCACGA
TTGAGTGTCA AAGAAGACCC TTCTTATGAC TCAGTCAGAA GAGGAGCTTG GGGCAATAAC
ATGAATTCTG GCCTCAACAA AAGTCCTCCC CTTGGAGGGG CACAAACGAT CAGTAAGAAT
ACAGAGCAAC GGCCCCAGCC AGATCCGTAT CAGATCCTGG GCCCGACCAG CAGTCGCCTA
GCCAACCCTG GAAGCGGGCA GATCCAGCTG TGGCAATTCC TCCTGGAGCT GCTCTCCGAC
AGCGCCAACG CCAGCTGTAT CACCTGGGAG GGGACCAACG GGGAGTTCAA AATGACGGAC
CCCGATGAGG TGGCCAGGCG CTGGGGCGAG CGGAAAAGCA AGCCCAACAT GAATTACGAC
AAGCTGAGCC GGGCCCTCCG TTATTACTAT GATAAAAACA TTATGACCAA AGTGCACGGC
AAAAGATATG CTTACAAATT TGACTTCCAC GGCATTGCCC AGGCTCTGCA GCCACATCCG
ACCGAGTCGT CCATGTACAA GTACCCTTCT GACATCTCCT ACATGCCTTC CTACCATGCC
CACCAGCAGA AGGTGAACTT TGTCCCTCCC CATCCATCCT CCATGCCTGT CACTTCCTCC
AGCTTCTTTG GAGCCGCATC ACAATACTGG ACCTCCCCCA CGGGGGGAAT CTACCCCAAC
CCCAACGTCC CCCGCCATCC TAACACCCAC GTGCCTTCAC ACTTAGGCAG CTACTACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57800
Clone ID Related Accession (Same CDS sequence) XM_011542702
Accession Version XM_011542702.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1233bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product Friend leukemia integration 1 transcription factor isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_033899.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGACGGGA CTATTAAGGA GGCTCTGTCG GTGGTGAGCG ACGACCAGTC CCTCTTTGAC 
TCAGCGTACG GAGCGGCAGC CCATCTCCCC AAGGCCGACA TGACTGCCTC GGGGAGTCCT
GACTACGGGC AGCCCCACAA GATCAACCCC CTCCCACCAC AGCAGGAGTG GATCAATCAG
CCAGTGAGGG TCAACGTCAA GCGGGAGTAT GACCACATGA ATGGATCCAG GGAGTCTCCG
GTGGACTGCA GCGTTAGCAA ATGCAGCAAG CTGGTGGGCG GAGGCGAGTC CAACCCCATG
AACTACAACA GCTATATGGA CGAGAAGAAT GGCCCCCCTC CTCCCAACAT GACCACCAAC
GAGAGGAGAG TCATCGTCCC CGCAGACCCC ACACTGTGGA CACAGGAGCA TGTGAGGCAA
TGGCTGGAGT GGGCCATAAA GGAGTACAGC TTGATGGAGA TCGACACATC CTTTTTCCAG
AACATGGATG GCAAGGAACT GTGTAAAATG AACAAGGAGG ACTTCCTCCG CGCCACCACC
CTCTACAACA CGGAAGTGCT GTTGTCACAC CTCAGTTACC TCAGGGAAAG TTCACTGCTG
GCCTATAATA CAACCTCCCA CACCGACCAA TCCTCACGAT TGAGTGTCAA AGAAGATCCG
TATCAGATCC TGGGCCCGAC CAGCAGTCGC CTAGCCAACC CTGGAAGCGG GCAGATCCAG
CTGTGGCAAT TCCTCCTGGA GCTGCTCTCC GACAGCGCCA ACGCCAGCTG TATCACCTGG
GAGGGGACCA ACGGGGAGTT CAAAATGACG GACCCCGATG AGGTGGCCAG GCGCTGGGGC
GAGCGGAAAA GCAAGCCCAA CATGAATTAC GACAAGCTGA GCCGGGCCCT CCGTTATTAC
TATGATAAAA ACATTATGAC CAAAGTGCAC GGCAAAAGAT ATGCTTACAA ATTTGACTTC
CACGGCATTG CCCAGGCTCT GCAGCCACAT CCGACCGAGT CGTCCATGTA CAAGTACCCT
TCTGACATCT CCTACATGCC TTCCTACCAT GCCCACCAGC AGAAGGTGAA CTTTGTCCCT
CCCCATCCAT CCTCCATGCC TGTCACTTCC TCCAGCTTCT TTGGAGCCGC ATCACAATAC
TGGACCTCCC CCACGGGGGG AATCTACCCC AACCCCAACG TCCCCCGCCA TCCTAACACC
CACGTGCCTT CACACTTAGG CAGCTACTAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011541004.1
CDS490..1722
Misc Feature(1)829..1101(+)
Misc Feature(2)1201..1452(+)
Translation

Target ORF information:

RefSeq Version XM_011542702
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Fli-1 proto-oncogene, ETS transcription factor (FLI1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011542702

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGACGGGA CTATTAAGGA GGCTCTGTCG GTGGTGAGCG ACGACCAGTC CCTCTTTGAC 
TCAGCGTACG GAGCGGCAGC CCATCTCCCC AAGGCCGACA TGACTGCCTC GGGGAGTCCT
GACTACGGGC AGCCCCACAA GATCAACCCC CTCCCACCAC AGCAGGAGTG GATCAATCAG
CCAGTGAGGG TCAACGTCAA GCGGGAGTAT GACCACATGA ATGGATCCAG GGAGTCTCCG
GTGGACTGCA GCGTTAGCAA ATGCAGCAAG CTGGTGGGCG GAGGCGAGTC CAACCCCATG
AACTACAACA GCTATATGGA CGAGAAGAAT GGCCCCCCTC CTCCCAACAT GACCACCAAC
GAGAGGAGAG TCATCGTCCC CGCAGACCCC ACACTGTGGA CACAGGAGCA TGTGAGGCAA
TGGCTGGAGT GGGCCATAAA GGAGTACAGC TTGATGGAGA TCGACACATC CTTTTTCCAG
AACATGGATG GCAAGGAACT GTGTAAAATG AACAAGGAGG ACTTCCTCCG CGCCACCACC
CTCTACAACA CGGAAGTGCT GTTGTCACAC CTCAGTTACC TCAGGGAAAG TTCACTGCTG
GCCTATAATA CAACCTCCCA CACCGACCAA TCCTCACGAT TGAGTGTCAA AGAAGATCCG
TATCAGATCC TGGGCCCGAC CAGCAGTCGC CTAGCCAACC CTGGAAGCGG GCAGATCCAG
CTGTGGCAAT TCCTCCTGGA GCTGCTCTCC GACAGCGCCA ACGCCAGCTG TATCACCTGG
GAGGGGACCA ACGGGGAGTT CAAAATGACG GACCCCGATG AGGTGGCCAG GCGCTGGGGC
GAGCGGAAAA GCAAGCCCAA CATGAATTAC GACAAGCTGA GCCGGGCCCT CCGTTATTAC
TATGATAAAA ACATTATGAC CAAAGTGCAC GGCAAAAGAT ATGCTTACAA ATTTGACTTC
CACGGCATTG CCCAGGCTCT GCAGCCACAT CCGACCGAGT CGTCCATGTA CAAGTACCCT
TCTGACATCT CCTACATGCC TTCCTACCAT GCCCACCAGC AGAAGGTGAA CTTTGTCCCT
CCCCATCCAT CCTCCATGCC TGTCACTTCC TCCAGCTTCT TTGGAGCCGC ATCACAATAC
TGGACCTCCC CCACGGGGGG AATCTACCCC AACCCCAACG TCCCCCGCCA TCCTAACACC
CACGTGCCTT CACACTTAGG CAGCTACTAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20383
Clone ID Related Accession (Same CDS sequence) NM_002017
Accession Version NM_002017.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1359bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product Friend leukemia integration 1 transcription factor isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC381599.1, AK313370.1, AP001122.5 and AI354789.1. This sequence is a reference standard in the RefSeqGene project. On Aug 22, 2012 this sequence version replaced gi:194018460. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M98833.3, X67001.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGACGGGA CTATTAAGGA GGCTCTGTCG GTGGTGAGCG ACGACCAGTC CCTCTTTGAC 
TCAGCGTACG GAGCGGCAGC CCATCTCCCC AAGGCCGACA TGACTGCCTC GGGGAGTCCT
GACTACGGGC AGCCCCACAA GATCAACCCC CTCCCACCAC AGCAGGAGTG GATCAATCAG
CCAGTGAGGG TCAACGTCAA GCGGGAGTAT GACCACATGA ATGGATCCAG GGAGTCTCCG
GTGGACTGCA GCGTTAGCAA ATGCAGCAAG CTGGTGGGCG GAGGCGAGTC CAACCCCATG
AACTACAACA GCTATATGGA CGAGAAGAAT GGCCCCCCTC CTCCCAACAT GACCACCAAC
GAGAGGAGAG TCATCGTCCC CGCAGACCCC ACACTGTGGA CACAGGAGCA TGTGAGGCAA
TGGCTGGAGT GGGCCATAAA GGAGTACAGC TTGATGGAGA TCGACACATC CTTTTTCCAG
AACATGGATG GCAAGGAACT GTGTAAAATG AACAAGGAGG ACTTCCTCCG CGCCACCACC
CTCTACAACA CGGAAGTGCT GTTGTCACAC CTCAGTTACC TCAGGGAAAG TTCACTGCTG
GCCTATAATA CAACCTCCCA CACCGACCAA TCCTCACGAT TGAGTGTCAA AGAAGACCCT
TCTTATGACT CAGTCAGAAG AGGAGCTTGG GGCAATAACA TGAATTCTGG CCTCAACAAA
AGTCCTCCCC TTGGAGGGGC ACAAACGATC AGTAAGAATA CAGAGCAACG GCCCCAGCCA
GATCCGTATC AGATCCTGGG CCCGACCAGC AGTCGCCTAG CCAACCCTGG AAGCGGGCAG
ATCCAGCTGT GGCAATTCCT CCTGGAGCTG CTCTCCGACA GCGCCAACGC CAGCTGTATC
ACCTGGGAGG GGACCAACGG GGAGTTCAAA ATGACGGACC CCGATGAGGT GGCCAGGCGC
TGGGGCGAGC GGAAAAGCAA GCCCAACATG AATTACGACA AGCTGAGCCG GGCCCTCCGT
TATTACTATG ATAAAAACAT TATGACCAAA GTGCACGGCA AAAGATATGC TTACAAATTT
GACTTCCACG GCATTGCCCA GGCTCTGCAG CCACATCCGA CCGAGTCGTC CATGTACAAG
TACCCTTCTG ACATCTCCTA CATGCCTTCC TACCATGCCC ACCAGCAGAA GGTGAACTTT
GTCCCTCCCC ATCCATCCTC CATGCCTGTC ACTTCCTCCA GCTTCTTTGG AGCCGCATCA
CAATACTGGA CCTCCCCCAC GGGGGGAATC TACCCCAACC CCAACGTCCC CCGCCATCCT
AACACCCACG TGCCTTCACA CTTAGGCAGC TACTACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002008.2
CDS344..1702
Misc Feature(1)263..265(+)
Misc Feature(2)683..955(+)
Misc Feature(3)1181..1432(+)
Exon (1)1..361
Gene:FLI1
Gene Synonym:
Exon (2)362..573
Gene:FLI1
Gene Synonym:
Exon (3)574..728
Gene:FLI1
Gene Synonym:
Exon (4)729..932
Gene:FLI1
Gene Synonym:
Exon (5)933..998
Gene:FLI1
Gene Synonym:
Exon (6)999..1064
Gene:FLI1
Gene Synonym:
Exon (7)1065..1124
Gene:FLI1
Gene Synonym:
Exon (8)1125..1172
Gene:FLI1
Gene Synonym:
Exon (9)1173..3981
Gene:FLI1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002017
Organism Homo sapiens (human)
Definition Homo sapiens Fli-1 proto-oncogene, ETS transcription factor (FLI1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002017

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGACGGGA CTATTAAGGA GGCTCTGTCG GTGGTGAGCG ACGACCAGTC CCTCTTTGAC 
TCAGCGTACG GAGCGGCAGC CCATCTCCCC AAGGCCGACA TGACTGCCTC GGGGAGTCCT
GACTACGGGC AGCCCCACAA GATCAACCCC CTCCCACCAC AGCAGGAGTG GATCAATCAG
CCAGTGAGGG TCAACGTCAA GCGGGAGTAT GACCACATGA ATGGATCCAG GGAGTCTCCG
GTGGACTGCA GCGTTAGCAA ATGCAGCAAG CTGGTGGGCG GAGGCGAGTC CAACCCCATG
AACTACAACA GCTATATGGA CGAGAAGAAT GGCCCCCCTC CTCCCAACAT GACCACCAAC
GAGAGGAGAG TCATCGTCCC CGCAGACCCC ACACTGTGGA CACAGGAGCA TGTGAGGCAA
TGGCTGGAGT GGGCCATAAA GGAGTACAGC TTGATGGAGA TCGACACATC CTTTTTCCAG
AACATGGATG GCAAGGAACT GTGTAAAATG AACAAGGAGG ACTTCCTCCG CGCCACCACC
CTCTACAACA CGGAAGTGCT GTTGTCACAC CTCAGTTACC TCAGGGAAAG TTCACTGCTG
GCCTATAATA CAACCTCCCA CACCGACCAA TCCTCACGAT TGAGTGTCAA AGAAGACCCT
TCTTATGACT CAGTCAGAAG AGGAGCTTGG GGCAATAACA TGAATTCTGG CCTCAACAAA
AGTCCTCCCC TTGGAGGGGC ACAAACGATC AGTAAGAATA CAGAGCAACG GCCCCAGCCA
GATCCGTATC AGATCCTGGG CCCGACCAGC AGTCGCCTAG CCAACCCTGG AAGCGGGCAG
ATCCAGCTGT GGCAATTCCT CCTGGAGCTG CTCTCCGACA GCGCCAACGC CAGCTGTATC
ACCTGGGAGG GGACCAACGG GGAGTTCAAA ATGACGGACC CCGATGAGGT GGCCAGGCGC
TGGGGCGAGC GGAAAAGCAA GCCCAACATG AATTACGACA AGCTGAGCCG GGCCCTCCGT
TATTACTATG ATAAAAACAT TATGACCAAA GTGCACGGCA AAAGATATGC TTACAAATTT
GACTTCCACG GCATTGCCCA GGCTCTGCAG CCACATCCGA CCGAGTCGTC CATGTACAAG
TACCCTTCTG ACATCTCCTA CATGCCTTCC TACCATGCCC ACCAGCAGAA GGTGAACTTT
GTCCCTCCCC ATCCATCCTC CATGCCTGTC ACTTCCTCCA GCTTCTTTGG AGCCGCATCA
CAATACTGGA CCTCCCCCAC GGGGGGAATC TACCCCAACC CCAACGTCCC CCGCCATCCT
AACACCCACG TGCCTTCACA CTTAGGCAGC TACTACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20623
Clone ID Related Accession (Same CDS sequence) NM_001271010
Accession Version NM_001271010.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1161bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product Friend leukemia integration 1 transcription factor isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC381599.1, M93255.1, AK313370.1, AP001122.5 and AI354789.1. This sequence is a reference standard in the RefSeqGene project. On Aug 31, 2013 this sequence version replaced gi:530397910. Transcript Variant: This variant (3) contains a distinct 5' UTR and 5' coding region, and uses an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct and shorter N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M93255.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146236, SAMEA2149398 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGAGGGAG GACTGGCAGG CGAGCGGGCG AGGGAGTCTC CGGTGGACTG CAGCGTTAGC 
AAATGCAGCA AGCTGGTGGG CGGAGGCGAG TCCAACCCCA TGAACTACAA CAGCTATATG
GACGAGAAGA ATGGCCCCCC TCCTCCCAAC ATGACCACCA ACGAGAGGAG AGTCATCGTC
CCCGCAGACC CCACACTGTG GACACAGGAG CATGTGAGGC AATGGCTGGA GTGGGCCATA
AAGGAGTACA GCTTGATGGA GATCGACACA TCCTTTTTCC AGAACATGGA TGGCAAGGAA
CTGTGTAAAA TGAACAAGGA GGACTTCCTC CGCGCCACCA CCCTCTACAA CACGGAAGTG
CTGTTGTCAC ACCTCAGTTA CCTCAGGGAA AGTTCACTGC TGGCCTATAA TACAACCTCC
CACACCGACC AATCCTCACG ATTGAGTGTC AAAGAAGACC CTTCTTATGA CTCAGTCAGA
AGAGGAGCTT GGGGCAATAA CATGAATTCT GGCCTCAACA AAAGTCCTCC CCTTGGAGGG
GCACAAACGA TCAGTAAGAA TACAGAGCAA CGGCCCCAGC CAGATCCGTA TCAGATCCTG
GGCCCGACCA GCAGTCGCCT AGCCAACCCT GGAAGCGGGC AGATCCAGCT GTGGCAATTC
CTCCTGGAGC TGCTCTCCGA CAGCGCCAAC GCCAGCTGTA TCACCTGGGA GGGGACCAAC
GGGGAGTTCA AAATGACGGA CCCCGATGAG GTGGCCAGGC GCTGGGGCGA GCGGAAAAGC
AAGCCCAACA TGAATTACGA CAAGCTGAGC CGGGCCCTCC GTTATTACTA TGATAAAAAC
ATTATGACCA AAGTGCACGG CAAAAGATAT GCTTACAAAT TTGACTTCCA CGGCATTGCC
CAGGCTCTGC AGCCACATCC GACCGAGTCG TCCATGTACA AGTACCCTTC TGACATCTCC
TACATGCCTT CCTACCATGC CCACCAGCAG AAGGTGAACT TTGTCCCTCC CCATCCATCC
TCCATGCCTG TCACTTCCTC CAGCTTCTTT GGAGCCGCAT CACAATACTG GACCTCCCCC
ACGGGGGGAA TCTACCCCAA CCCCAACGTC CCCCGCCATC CTAACACCCA CGTGCCTTCA
CACTTAGGCA GCTACTACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001257939.1
CDS713..1873
Misc Feature(1)632..634(+)
Misc Feature(2)854..1126(+)
Misc Feature(3)1352..1603(+)
Exon (1)1..361
Gene:FLI1
Gene Synonym:
Exon (2)362..573
Gene:FLI1
Gene Synonym:
Exon (3)574..744
Gene:FLI1
Gene Synonym:
Exon (4)745..899
Gene:FLI1
Gene Synonym:
Exon (5)900..1103
Gene:FLI1
Gene Synonym:
Exon (6)1104..1169
Gene:FLI1
Gene Synonym:
Exon (7)1170..1235
Gene:FLI1
Gene Synonym:
Exon (8)1236..1295
Gene:FLI1
Gene Synonym:
Exon (9)1296..1343
Gene:FLI1
Gene Synonym:
Exon (10)1344..4152
Gene:FLI1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001271010
Organism Homo sapiens (human)
Definition Homo sapiens Fli-1 proto-oncogene, ETS transcription factor (FLI1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001271010

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGAGGGAG GACTGGCAGG CGAGCGGGCG AGGGAGTCTC CGGTGGACTG CAGCGTTAGC 
AAATGCAGCA AGCTGGTGGG CGGAGGCGAG TCCAACCCCA TGAACTACAA CAGCTATATG
GACGAGAAGA ATGGCCCCCC TCCTCCCAAC ATGACCACCA ACGAGAGGAG AGTCATCGTC
CCCGCAGACC CCACACTGTG GACACAGGAG CATGTGAGGC AATGGCTGGA GTGGGCCATA
AAGGAGTACA GCTTGATGGA GATCGACACA TCCTTTTTCC AGAACATGGA TGGCAAGGAA
CTGTGTAAAA TGAACAAGGA GGACTTCCTC CGCGCCACCA CCCTCTACAA CACGGAAGTG
CTGTTGTCAC ACCTCAGTTA CCTCAGGGAA AGTTCACTGC TGGCCTATAA TACAACCTCC
CACACCGACC AATCCTCACG ATTGAGTGTC AAAGAAGACC CTTCTTATGA CTCAGTCAGA
AGAGGAGCTT GGGGCAATAA CATGAATTCT GGCCTCAACA AAAGTCCTCC CCTTGGAGGG
GCACAAACGA TCAGTAAGAA TACAGAGCAA CGGCCCCAGC CAGATCCGTA TCAGATCCTG
GGCCCGACCA GCAGTCGCCT AGCCAACCCT GGAAGCGGGC AGATCCAGCT GTGGCAATTC
CTCCTGGAGC TGCTCTCCGA CAGCGCCAAC GCCAGCTGTA TCACCTGGGA GGGGACCAAC
GGGGAGTTCA AAATGACGGA CCCCGATGAG GTGGCCAGGC GCTGGGGCGA GCGGAAAAGC
AAGCCCAACA TGAATTACGA CAAGCTGAGC CGGGCCCTCC GTTATTACTA TGATAAAAAC
ATTATGACCA AAGTGCACGG CAAAAGATAT GCTTACAAAT TTGACTTCCA CGGCATTGCC
CAGGCTCTGC AGCCACATCC GACCGAGTCG TCCATGTACA AGTACCCTTC TGACATCTCC
TACATGCCTT CCTACCATGC CCACCAGCAG AAGGTGAACT TTGTCCCTCC CCATCCATCC
TCCATGCCTG TCACTTCCTC CAGCTTCTTT GGAGCCGCAT CACAATACTG GACCTCCCCC
ACGGGGGGAA TCTACCCCAA CCCCAACGTC CCCCGCCATC CTAACACCCA CGTGCCTTCA
CACTTAGGCA GCTACTACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21072
Clone ID Related Accession (Same CDS sequence) NM_001271012
Accession Version NM_001271012.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 780bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product Friend leukemia integration 1 transcription factor isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK294279.1, AP001122.5 and AI354789.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region and two alternate internal exons, and uses an alternate start codon, compared to variant 1. The encoded isoform (4) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK294279.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2155751 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGATCCAG GTTCACTGCT GGCCTATAAT ACAACCTCCC ACACCGACCA ATCCTCACGA 
TTGAGTGTCA AAGAAGACCC TTCTTATGAC TCAGTCAGAA GAGGAGCTTG GGGCAATAAC
ATGAATTCTG GCCTCAACAA AAGTCCTCCC CTTGGAGGGG CACAAACGAT CAGTAAGAAT
ACAGAGCAAC GGCCCCAGCC AGATCCGTAT CAGATCCTGG GCCCGACCAG CAGTCGCCTA
GCCAACCCTG GAAGCGGGCA GATCCAGCTG TGGCAATTCC TCCTGGAGCT GCTCTCCGAC
AGCGCCAACG CCAGCTGTAT CACCTGGGAG GGGACCAACG GGGAGTTCAA AATGACGGAC
CCCGATGAGG TGGCCAGGCG CTGGGGCGAG CGGAAAAGCA AGCCCAACAT GAATTACGAC
AAGCTGAGCC GGGCCCTCCG TTATTACTAT GATAAAAACA TTATGACCAA AGTGCACGGC
AAAAGATATG CTTACAAATT TGACTTCCAC GGCATTGCCC AGGCTCTGCA GCCACATCCG
ACCGAGTCGT CCATGTACAA GTACCCTTCT GACATCTCCT ACATGCCTTC CTACCATGCC
CACCAGCAGA AGGTGAACTT TGTCCCTCCC CATCCATCCT CCATGCCTGT CACTTCCTCC
AGCTTCTTTG GAGCCGCATC ACAATACTGG ACCTCCCCCA CGGGGGGAAT CTACCCCAAC
CCCAACGTCC CCCGCCATCC TAACACCCAC GTGCCTTCAC ACTTAGGCAG CTACTACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001257941.1
CDS370..1149
Misc Feature(1)367..369(+)
Misc Feature(2)628..879(+)
Exon (1)1..167
Gene:FLI1
Gene Synonym:
Exon (2)168..379
Gene:FLI1
Gene Synonym:
Exon (3)380..445
Gene:FLI1
Gene Synonym:
Exon (4)446..511
Gene:FLI1
Gene Synonym:
Exon (5)512..571
Gene:FLI1
Gene Synonym:
Exon (6)572..619
Gene:FLI1
Gene Synonym:
Exon (7)620..3428
Gene:FLI1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001271012
Organism Homo sapiens (human)
Definition Homo sapiens Fli-1 proto-oncogene, ETS transcription factor (FLI1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001271012

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGATCCAG GTTCACTGCT GGCCTATAAT ACAACCTCCC ACACCGACCA ATCCTCACGA 
TTGAGTGTCA AAGAAGACCC TTCTTATGAC TCAGTCAGAA GAGGAGCTTG GGGCAATAAC
ATGAATTCTG GCCTCAACAA AAGTCCTCCC CTTGGAGGGG CACAAACGAT CAGTAAGAAT
ACAGAGCAAC GGCCCCAGCC AGATCCGTAT CAGATCCTGG GCCCGACCAG CAGTCGCCTA
GCCAACCCTG GAAGCGGGCA GATCCAGCTG TGGCAATTCC TCCTGGAGCT GCTCTCCGAC
AGCGCCAACG CCAGCTGTAT CACCTGGGAG GGGACCAACG GGGAGTTCAA AATGACGGAC
CCCGATGAGG TGGCCAGGCG CTGGGGCGAG CGGAAAAGCA AGCCCAACAT GAATTACGAC
AAGCTGAGCC GGGCCCTCCG TTATTACTAT GATAAAAACA TTATGACCAA AGTGCACGGC
AAAAGATATG CTTACAAATT TGACTTCCAC GGCATTGCCC AGGCTCTGCA GCCACATCCG
ACCGAGTCGT CCATGTACAA GTACCCTTCT GACATCTCCT ACATGCCTTC CTACCATGCC
CACCAGCAGA AGGTGAACTT TGTCCCTCCC CATCCATCCT CCATGCCTGT CACTTCCTCC
AGCTTCTTTG GAGCCGCATC ACAATACTGG ACCTCCCCCA CGGGGGGAAT CTACCCCAAC
CCCAACGTCC CCCGCCATCC TAACACCCAC GTGCCTTCAC ACTTAGGCAG CTACTACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20678
Clone ID Related Accession (Same CDS sequence) NM_001167681 , XM_011542701
Accession Version NM_001167681.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1260bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product Friend leukemia integration 1 transcription factor isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC381599.1, AK300153.1, AP001122.5 and AI354789.1. This sequence is a reference standard in the RefSeqGene project. On Apr 8, 2011 this sequence version replaced gi:264681553. Transcript Variant: This variant (2) contains a distinct 5' UTR and uses a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300153.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGACTGCCT CGGGGAGTCC TGACTACGGG CAGCCCCACA AGATCAACCC CCTCCCACCA 
CAGCAGGAGT GGATCAATCA GCCAGTGAGG GTCAACGTCA AGCGGGAGTA TGACCACATG
AATGGATCCA GGGAGTCTCC GGTGGACTGC AGCGTTAGCA AATGCAGCAA GCTGGTGGGC
GGAGGCGAGT CCAACCCCAT GAACTACAAC AGCTATATGG ACGAGAAGAA TGGCCCCCCT
CCTCCCAACA TGACCACCAA CGAGAGGAGA GTCATCGTCC CCGCAGACCC CACACTGTGG
ACACAGGAGC ATGTGAGGCA ATGGCTGGAG TGGGCCATAA AGGAGTACAG CTTGATGGAG
ATCGACACAT CCTTTTTCCA GAACATGGAT GGCAAGGAAC TGTGTAAAAT GAACAAGGAG
GACTTCCTCC GCGCCACCAC CCTCTACAAC ACGGAAGTGC TGTTGTCACA CCTCAGTTAC
CTCAGGGAAA GTTCACTGCT GGCCTATAAT ACAACCTCCC ACACCGACCA ATCCTCACGA
TTGAGTGTCA AAGAAGACCC TTCTTATGAC TCAGTCAGAA GAGGAGCTTG GGGCAATAAC
ATGAATTCTG GCCTCAACAA AAGTCCTCCC CTTGGAGGGG CACAAACGAT CAGTAAGAAT
ACAGAGCAAC GGCCCCAGCC AGATCCGTAT CAGATCCTGG GCCCGACCAG CAGTCGCCTA
GCCAACCCTG GAAGCGGGCA GATCCAGCTG TGGCAATTCC TCCTGGAGCT GCTCTCCGAC
AGCGCCAACG CCAGCTGTAT CACCTGGGAG GGGACCAACG GGGAGTTCAA AATGACGGAC
CCCGATGAGG TGGCCAGGCG CTGGGGCGAG CGGAAAAGCA AGCCCAACAT GAATTACGAC
AAGCTGAGCC GGGCCCTCCG TTATTACTAT GATAAAAACA TTATGACCAA AGTGCACGGC
AAAAGATATG CTTACAAATT TGACTTCCAC GGCATTGCCC AGGCTCTGCA GCCACATCCG
ACCGAGTCGT CCATGTACAA GTACCCTTCT GACATCTCCT ACATGCCTTC CTACCATGCC
CACCAGCAGA AGGTGAACTT TGTCCCTCCC CATCCATCCT CCATGCCTGT CACTTCCTCC
AGCTTCTTTG GAGCCGCATC ACAATACTGG ACCTCCCCCA CGGGGGGAAT CTACCCCAAC
CCCAACGTCC CCCGCCATCC TAACACCCAC GTGCCTTCAC ACTTAGGCAG CTACTACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001161153.1
CDS497..1756
Misc Feature(1)389..391(+)
Misc Feature(2)737..1009(+)
Misc Feature(3)1235..1486(+)
Exon (1)1..361
Gene:FLI1
Gene Synonym:
Exon (2)362..415
Gene:FLI1
Gene Synonym:
Exon (3)416..627
Gene:FLI1
Gene Synonym:
Exon (4)628..782
Gene:FLI1
Gene Synonym:
Exon (5)783..986
Gene:FLI1
Gene Synonym:
Exon (6)987..1052
Gene:FLI1
Gene Synonym:
Exon (7)1053..1118
Gene:FLI1
Gene Synonym:
Exon (8)1119..1178
Gene:FLI1
Gene Synonym:
Exon (9)1179..1226
Gene:FLI1
Gene Synonym:
Exon (10)1227..4035
Gene:FLI1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001167681
Organism Homo sapiens (human)
Definition Homo sapiens Fli-1 proto-oncogene, ETS transcription factor (FLI1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001167681

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGACTGCCT CGGGGAGTCC TGACTACGGG CAGCCCCACA AGATCAACCC CCTCCCACCA 
CAGCAGGAGT GGATCAATCA GCCAGTGAGG GTCAACGTCA AGCGGGAGTA TGACCACATG
AATGGATCCA GGGAGTCTCC GGTGGACTGC AGCGTTAGCA AATGCAGCAA GCTGGTGGGC
GGAGGCGAGT CCAACCCCAT GAACTACAAC AGCTATATGG ACGAGAAGAA TGGCCCCCCT
CCTCCCAACA TGACCACCAA CGAGAGGAGA GTCATCGTCC CCGCAGACCC CACACTGTGG
ACACAGGAGC ATGTGAGGCA ATGGCTGGAG TGGGCCATAA AGGAGTACAG CTTGATGGAG
ATCGACACAT CCTTTTTCCA GAACATGGAT GGCAAGGAAC TGTGTAAAAT GAACAAGGAG
GACTTCCTCC GCGCCACCAC CCTCTACAAC ACGGAAGTGC TGTTGTCACA CCTCAGTTAC
CTCAGGGAAA GTTCACTGCT GGCCTATAAT ACAACCTCCC ACACCGACCA ATCCTCACGA
TTGAGTGTCA AAGAAGACCC TTCTTATGAC TCAGTCAGAA GAGGAGCTTG GGGCAATAAC
ATGAATTCTG GCCTCAACAA AAGTCCTCCC CTTGGAGGGG CACAAACGAT CAGTAAGAAT
ACAGAGCAAC GGCCCCAGCC AGATCCGTAT CAGATCCTGG GCCCGACCAG CAGTCGCCTA
GCCAACCCTG GAAGCGGGCA GATCCAGCTG TGGCAATTCC TCCTGGAGCT GCTCTCCGAC
AGCGCCAACG CCAGCTGTAT CACCTGGGAG GGGACCAACG GGGAGTTCAA AATGACGGAC
CCCGATGAGG TGGCCAGGCG CTGGGGCGAG CGGAAAAGCA AGCCCAACAT GAATTACGAC
AAGCTGAGCC GGGCCCTCCG TTATTACTAT GATAAAAACA TTATGACCAA AGTGCACGGC
AAAAGATATG CTTACAAATT TGACTTCCAC GGCATTGCCC AGGCTCTGCA GCCACATCCG
ACCGAGTCGT CCATGTACAA GTACCCTTCT GACATCTCCT ACATGCCTTC CTACCATGCC
CACCAGCAGA AGGTGAACTT TGTCCCTCCC CATCCATCCT CCATGCCTGT CACTTCCTCC
AGCTTCTTTG GAGCCGCATC ACAATACTGG ACCTCCCCCA CGGGGGGAAT CTACCCCAAC
CCCAACGTCC CCCGCCATCC TAACACCCAC GTGCCTTCAC ACTTAGGCAG CTACTACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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