FN1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FN1
Entrez Gene ID	2335
Full Name	fibronectin 1
Synonyms	CIG,ED-B,FINC,FN,FNZ,GFND,GFND2,LETS,MSF,SMDCF
General protein information	Preferred Names fibronectin 1 Names fibronectin cold-insoluble globulin migration-stimulating factor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q35
Summary	This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016].
Disorder MIM:	135600
Disorder Html:	Glomerulopathy with fibronectin deposits 2, 601894 (3); Plasma fibronectin deficiency, 614101 (1)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017003693.1	XP_016859182.1	fibronectin isoform X14
XM_005246405.1	XP_005246462.1	fibronectin isoform X8
XM_005246398.1	XP_005246455.1	fibronectin isoform X2
XM_005246399.1	XP_005246456.1	fibronectin isoform X3
XM_024452769.1	XP_024308537.1	fibronectin isoform X4
XM_005246403.1	XP_005246460.1	fibronectin isoform X7
XM_024452770.1	XP_024308538.1	fibronectin isoform X11
XM_005246410.1	XP_005246467.1	fibronectin isoform X18
XM_005246411.1	XP_005246468.1	fibronectin isoform X21
NM_212476.2	NP_997641.1	fibronectin isoform 5 precursor
NM_001306132.1	NP_001293061.1	fibronectin isoform 11 precursor
NM_054034.2	NP_473375.2	fibronectin isoform 7 preproprotein
NM_001306131.1	NP_001293060.1	fibronectin isoform 10 precursor
NM_001365518.1	NP_001352447.1	fibronectin isoform 13 precursor
NM_002026.3	NP_002017.1	fibronectin isoform 3 preproprotein
NM_001365520.1	NP_001352449.1	fibronectin isoform 15 precursor
NM_001365522.1	NP_001352451.1	fibronectin isoform 17 precursor
XM_005246412.1	XP_005246469.1	fibronectin isoform X19
XM_005246407.1	XP_005246464.1	fibronectin isoform X12
XM_005246408.1	XP_005246465.1	fibronectin isoform X13
XM_017003692.1	XP_016859181.1	fibronectin isoform X16
XM_017003695.1	XP_016859184.1	fibronectin isoform X20
NM_212478.2	NP_997643.1	fibronectin isoform 4 precursor
NM_212482.3	NP_997647.1	fibronectin isoform 1 precursor
NM_001365519.1	NP_001352448.1	fibronectin isoform 14 precursor
NM_212482.1	NP_997647.1	fibronectin isoform 1 preproprotein
XM_005246409.1	XP_005246466.1	fibronectin isoform X12
XM_005246401.1	XP_005246458.1	fibronectin isoform X5
XM_005246404.1	XP_005246461.1	fibronectin isoform X8
XM_017003694.1	XP_016859183.1	fibronectin isoform X16
XM_005246402.1	XP_005246459.1	fibronectin isoform X6
XM_005246416.1	XP_005246473.1	fibronectin isoform X24
NM_001306129.1	NP_001293058.1	fibronectin isoform 8 precursor
NM_001365524.1	NP_001352453.1	fibronectin isoform 19 precursor
NM_001365523.1	NP_001352452.1	fibronectin isoform 18 precursor
NM_001365517.1	NP_001352446.1	fibronectin isoform 12 precursor
XM_005246414.1	XP_005246471.1	fibronectin isoform X20
NM_212482.2	NP_997647.1	fibronectin isoform 1 precursor
XM_005246406.1	XP_005246463.1	fibronectin isoform X9
XM_005246397.1	XP_005246454.1	fibronectin isoform X1
NM_001365521.1	NP_001352450.1	fibronectin isoform 16 precursor
NM_001306130.1	NP_001293059.1	fibronectin isoform 9 precursor
NM_212474.2	NP_997639.1	fibronectin isoform 6 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FN1	XP_516072.3
	FN1	XP_001083548.2
Mus musculus (house mouse)	Fn1	NP_034363.1
Gallus gallus (chicken)	FN1	NP_001185641.1
Danio rerio (zebrafish)	fn1a	NP_571595.1
Homo sapiens (human)	FN1	NP_997647.1
Canis lupus familiaris (dog)	FN1	XP_005640798.1
Rattus norvegicus (Norway rat)	Fn1	NP_062016.2
Xenopus tropicalis (tropical clawed frog)	fn1	NP_001025695.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in Fibronectin Cause a Subtype of Spondylometaphyseal Dysplasia with "Corner Fractures".
Lee CS, Fu H, Baratang N, Rousseau J, Kumra H, Sutton VR, Niceta M, Ciolfi A, Yamamoto G, Bertola D, Marcelis CL, Lugtenberg D, Bartuli A, Kim C, Hoover-Fong J, Sobreira N, Pauli R, Bacino C, Krakow D, Parboosingh J, Yap P, Kariminejad A, McDonald MT, Aracena MI, Lausch E, Unger S, Superti-Furga A, Lu JT, , Cohn DH, Tartaglia M, Lee BH, Reinhardt DP, Campeau PM
American journal of human genetics101(5)815-823(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cancer-associated fibroblasts organize the fibronectin matrix and promote directional prostate cancer cell migration.
Title: Cancer-associated fibroblasts promote directional cancer cell migration by aligning fibronectin.

FN1 mutations that cause defective fibronectin secretion are found in SMD.
Title: Mutations in Fibronectin Cause a Subtype of Spondylometaphyseal Dysplasia with "Corner Fractures".

Thyroid hormone T3 induces fibronectin and HIF-1alpha synthesis via PI3K/AKT signaling pathway.
Title: Thyroid hormone regulates fibronectin expression through the activation of the hypoxia inducible factor 1.

If the expression of Capon is decreased, myeloma cells are adhered to fibronectin or bone marrow stromal cells (bone marrow mesenchymal stem cells). In addition, the sensitivity of the cell line to chemotherapeutic agents was reduced after silencing Capon in the myeloma cell line which was adhered to bone marrow mesenchymal stem cells.
Title: The role of Capon in multiple myeloma.

Study identifies four likely Tourette disorder risk genes with multiple de novo damaging variants in unrelated probands: WWC1 (WW and C2 domain containing 1), CELSR3 (Cadherin EGF LAG seven-pass G-type receptor 3), NIPBL (Nipped-B-like), and FN1 (fibronectin 1).
Title: De Novo Coding Variants Are Strongly Associated with Tourette Disorder.

The following FN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu24787	NM_212482.3 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu24809	NM_054034.2 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu107507	NM_001365519.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant 14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu89216	NM_001365521.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant 16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu89217	NM_001365522.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant 17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu104833	XM_024452769.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu104834	XM_024452770.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu89215	XM_017003692.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu89216	XM_017003693.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu89217	XM_017003694.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu89218	XM_017003695.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu24915	NM_212476.2 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu24997	NM_212474.2 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu25005	NM_002026.3 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu25016	NM_212478.2 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu36020	NM_001365517.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant 12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu36021	NM_001365518.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant 13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu36024	NM_001365520.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant 15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu36027	NM_001365523.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant 18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36029	NM_001365524.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant 19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu57835	NM_001306131.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu57836	NM_001306129.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu57837	NM_001306130.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu57838	NM_001306132.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36012	XM_005246397.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu36013	XM_005246398.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu36014	XM_005246399.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu36016	XM_005246401.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu36017	XM_005246402.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu36018	XM_005246403.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu36019	XM_005246404.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu36020	XM_005246405.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu36021	XM_005246406.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu36022	XM_005246407.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu36023	XM_005246408.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu36024	XM_005246409.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu36025	XM_005246410.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu36026	XM_005246411.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36027	XM_005246412.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36029	XM_005246414.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36031	XM_005246416.1 Latest version!	Homo sapiens fibronectin 1 (FN1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu24787	NM_212482.1	Homo sapiens fibronectin 1 (FN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu24787	NM_212482.2	Homo sapiens fibronectin 1 (FN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FN1 ( NM_212482.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_997647.1 Homo sapiens fibronectin 1 (FN1), transcript variant 1, mRNA.

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CloneID	OHu24787
Clone ID Related Accession (Same CDS sequence)	NM_212482.3 , NM_212482.1 , NM_212482.2
Accession Version	NM_212482.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	fibronectin isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX640803.1, AJ535086.1, BX640875.1, AI033037.1, U60068.1, BX640999.1, BQ005645.1 and CR749317.1. On Nov 22, 2018 this sequence version replaced NM_212482.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). The encoded isoform (1) may undergo proteolytic processing similar to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX640803.1, BX640875.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT 
CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT 
CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA 
CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA 
GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT 
CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT 
ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT 
CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT 
GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT 
GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA 
CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA 
AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA 
ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA 
ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT 
GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG 
CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG 
GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG 
TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC 
ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC 
CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA 
GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC 
ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC 
ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT 
CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC 
ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT 
GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG 
CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG 
ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT 
CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT 
ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA 
AAGACAGACG AGCTTCCCCA ACTGGTAACC CTTCCACACC CCAATCTTCA TGGACCAGAG 
ATCTTGGATG TTCCTTCCAC AGTTCAAAAG ACCCCTTTCG TCACCCACCC TGGGTATGAC 
ACTGGAAATG GTATTCAGCT TCCTGGCACT TCTGGTCAGC AACCCAGTGT TGGGCAACAA 
ATGATCTTTG AGGAACATGG TTTTAGGCGG ACCACACCGC CCACAACGGC CACCCCCATA 
AGGCATAGGC CAAGACCATA CCCGCCGAAT GTAGGTGAGG AAATCCAAAT TGGTCACATC 
CCCAGGGAAG ATGTAGACTA TCACCTGTAC CCACACGGTC CGGGACTCAA TCCAAATGCC 
TCTACAGGAC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGGCCCCATT CCAGGACACT 
TCTGAGTACA TCATTTCATG TCATCCTGTT GGCACTGATG AAGAACCCTT ACAGTTCAGG 
GTTCCTGGAA CTTCTACCAG TGCCACTCTG ACAGGCCTCA CCAGAGGTGC CACCTACAAC 
ATCATAGTGG AGGCACTGAA AGACCAGCAG AGGCATAAGG TTCGGGAAGA GGTTGTTACC 
GTGGGCAACT CTGTCAACGA AGGCTTGAAC CAACCTACGG ATGACTCGTG CTTTGACCCC 
TACACAGTTT CCCATTATGC CGTTGGAGAT GAGTGGGAAC GAATGTCTGA ATCAGGCTTT 
AAACTGTTGT GCCAGTGCTT AGGCTTTGGA AGTGGTCATT TCAGATGTGA TTCATCTAGA 
TGGTGCCATG ACAATGGTGT GAACTACAAG ATTGGAGAGA AGTGGGACCG TCAGGGAGAA 
AATGGCCAGA TGATGAGCTG CACATGTCTT GGGAACGGAA AAGGAGAATT CAAGTGTGAC 
CCTCATGAGG CAACGTGTTA TGATGATGGG AAGACATACC ACGTAGGAGA ACAGTGGCAG 
AAGGAATATC TCGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC 
TGTGACAACT GCCGCAGACC TGGGGGTGAA CCCAGTCCCG AAGGCACTAC TGGCCAGTCC 
TACAACCAGT ATTCTCAGAG ATACCATCAG AGAACAAACA CTAATGTTAA TTGCCCAATT 
GAGTGCTTCA TGCCTTTAGA TGTACAGGCT GACAGAGAAG ATTCCCGAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997647.1
CDS	267..7700
Translation	MLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDPTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSGESQPLVQTAVTNIDRPKGLAFTDVDVDSIKIAWESPQGQVSRYRVTYSSPEDGIHELFPAPDGEEDTAELQGLRPGSEYTVSVVALHDDMESQPLIGTQSTAIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPSTVQKTPFVTHPGYDTGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPIRHRPRPYPPNVGEEIQIGHIPREDVDYHLYPHGPGLNPNASTGQEALSQTTISWAPFQDTSEYIISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGATYNIIVEALKDQQRHKVREEVVTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWERMSESGFKLLCQCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVNCPIECFMPLDVQADREDSRE

Target ORF information:

RefSeq Version	NM_212482.3
Organism	Homo sapiens(human)
Definition	Homo sapiens fibronectin 1 (FN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212482.3

ORF Insert Sequence:

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT 
CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT 
CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA 
CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA 
GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT 
CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT 
ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT 
CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT 
GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT 
GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA 
CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA 
AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA 
ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA 
ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT 
GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG 
CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG 
GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG 
TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC 
ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC 
CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA 
GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC 
ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC 
ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT 
CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC 
ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT 
GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG 
CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG 
ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT 
CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT 
ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA 
AAGACAGACG AGCTTCCCCA ACTGGTAACC CTTCCACACC CCAATCTTCA TGGACCAGAG 
ATCTTGGATG TTCCTTCCAC AGTTCAAAAG ACCCCTTTCG TCACCCACCC TGGGTATGAC 
ACTGGAAATG GTATTCAGCT TCCTGGCACT TCTGGTCAGC AACCCAGTGT TGGGCAACAA 
ATGATCTTTG AGGAACATGG TTTTAGGCGG ACCACACCGC CCACAACGGC CACCCCCATA 
AGGCATAGGC CAAGACCATA CCCGCCGAAT GTAGGTGAGG AAATCCAAAT TGGTCACATC 
CCCAGGGAAG ATGTAGACTA TCACCTGTAC CCACACGGTC CGGGACTCAA TCCAAATGCC 
TCTACAGGAC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGGCCCCATT CCAGGACACT 
TCTGAGTACA TCATTTCATG TCATCCTGTT GGCACTGATG AAGAACCCTT ACAGTTCAGG 
GTTCCTGGAA CTTCTACCAG TGCCACTCTG ACAGGCCTCA CCAGAGGTGC CACCTACAAC 
ATCATAGTGG AGGCACTGAA AGACCAGCAG AGGCATAAGG TTCGGGAAGA GGTTGTTACC 
GTGGGCAACT CTGTCAACGA AGGCTTGAAC CAACCTACGG ATGACTCGTG CTTTGACCCC 
TACACAGTTT CCCATTATGC CGTTGGAGAT GAGTGGGAAC GAATGTCTGA ATCAGGCTTT 
AAACTGTTGT GCCAGTGCTT AGGCTTTGGA AGTGGTCATT TCAGATGTGA TTCATCTAGA 
TGGTGCCATG ACAATGGTGT GAACTACAAG ATTGGAGAGA AGTGGGACCG TCAGGGAGAA 
AATGGCCAGA TGATGAGCTG CACATGTCTT GGGAACGGAA AAGGAGAATT CAAGTGTGAC 
CCTCATGAGG CAACGTGTTA TGATGATGGG AAGACATACC ACGTAGGAGA ACAGTGGCAG 
AAGGAATATC TCGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC 
TGTGACAACT GCCGCAGACC TGGGGGTGAA CCCAGTCCCG AAGGCACTAC TGGCCAGTCC 
TACAACCAGT ATTCTCAGAG ATACCATCAG AGAACAAACA CTAATGTTAA TTGCCCAATT 
GAGTGCTTCA TGCCTTTAGA TGTACAGGCT GACAGAGAAG ATTCCCGAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( NM_212482.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_997647.1 Homo sapiens fibronectin 1 (FN1), transcript variant 1, mRNA.

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CloneID	OHu24787
Clone ID Related Accession (Same CDS sequence)	NM_212482.3 , NM_212482.1 , NM_212482.2
Accession Version	NM_212482.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-14
Organism	Homo sapiens(Human)
Product	fibronectin isoform 1 preproprotein
Comment

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT 
CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT 
CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA 
CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA 
GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT 
CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT 
ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT 
CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT 
GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT 
GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA 
CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA 
AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA 
ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA 
ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT 
GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG 
CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG 
GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG 
TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC 
ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC 
CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA 
GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC 
ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC 
ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT 
CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC 
ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT 
GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG 
CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG 
ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT 
CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT 
ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA 
AAGACAGACG AGCTTCCCCA ACTGGTAACC CTTCCACACC CCAATCTTCA TGGACCAGAG 
ATCTTGGATG TTCCTTCCAC AGTTCAAAAG ACCCCTTTCG TCACCCACCC TGGGTATGAC 
ACTGGAAATG GTATTCAGCT TCCTGGCACT TCTGGTCAGC AACCCAGTGT TGGGCAACAA 
ATGATCTTTG AGGAACATGG TTTTAGGCGG ACCACACCGC CCACAACGGC CACCCCCATA 
AGGCATAGGC CAAGACCATA CCCGCCGAAT GTAGGTGAGG AAATCCAAAT TGGTCACATC 
CCCAGGGAAG ATGTAGACTA TCACCTGTAC CCACACGGTC CGGGACTCAA TCCAAATGCC 
TCTACAGGAC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGGCCCCATT CCAGGACACT 
TCTGAGTACA TCATTTCATG TCATCCTGTT GGCACTGATG AAGAACCCTT ACAGTTCAGG 
GTTCCTGGAA CTTCTACCAG TGCCACTCTG ACAGGCCTCA CCAGAGGTGC CACCTACAAC 
ATCATAGTGG AGGCACTGAA AGACCAGCAG AGGCATAAGG TTCGGGAAGA GGTTGTTACC 
GTGGGCAACT CTGTCAACGA AGGCTTGAAC CAACCTACGG ATGACTCGTG CTTTGACCCC 
TACACAGTTT CCCATTATGC CGTTGGAGAT GAGTGGGAAC GAATGTCTGA ATCAGGCTTT 
AAACTGTTGT GCCAGTGCTT AGGCTTTGGA AGTGGTCATT TCAGATGTGA TTCATCTAGA 
TGGTGCCATG ACAATGGTGT GAACTACAAG ATTGGAGAGA AGTGGGACCG TCAGGGAGAA 
AATGGCCAGA TGATGAGCTG CACATGTCTT GGGAACGGAA AAGGAGAATT CAAGTGTGAC 
CCTCATGAGG CAACGTGTTA TGATGATGGG AAGACATACC ACGTAGGAGA ACAGTGGCAG 
AAGGAATATC TCGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC 
TGTGACAACT GCCGCAGACC TGGGGGTGAA CCCAGTCCCG AAGGCACTAC TGGCCAGTCC 
TACAACCAGT ATTCTCAGAG ATACCATCAG AGAACAAACA CTAATGTTAA TTGCCCAATT 
GAGTGCTTCA TGCCTTTAGA TGTACAGGCT GACAGAGAAG ATTCCCGAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_997647.1

Translation

MLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDPTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSGESQPLVQTAVTNIDRPKGLAFTDVDVDSIKIAWESPQGQVSRYRVTYSSPEDGIHELFPAPDGEEDTAELQGLRPGSEYTVSVVALHDDMESQPLIGTQSTAIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPSTVQKTPFVTHPGYDTGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPIRHRPRPYPPNVGEEIQIGHIPREDVDYHLYPHGPGLNPNASTGQEALSQTTISWAPFQDTSEYIISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGATYNIIVEALKDQQRHKVREEVVTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWERMSESGFKLLCQCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVNCPIECFMPLDVQADREDSRE

Target ORF information:

RefSeq Version	NM_212482.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens fibronectin 1 (FN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212482.1

ORF Insert Sequence:

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT 
CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT 
CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA 
CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA 
GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT 
CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT 
ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT 
CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT 
GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT 
GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA 
CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA 
AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA 
ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA 
ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT 
GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG 
CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG 
GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG 
TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC 
ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC 
CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA 
GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC 
ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC 
ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT 
CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC 
ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT 
GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG 
CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG 
ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT 
CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT 
ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA 
AAGACAGACG AGCTTCCCCA ACTGGTAACC CTTCCACACC CCAATCTTCA TGGACCAGAG 
ATCTTGGATG TTCCTTCCAC AGTTCAAAAG ACCCCTTTCG TCACCCACCC TGGGTATGAC 
ACTGGAAATG GTATTCAGCT TCCTGGCACT TCTGGTCAGC AACCCAGTGT TGGGCAACAA 
ATGATCTTTG AGGAACATGG TTTTAGGCGG ACCACACCGC CCACAACGGC CACCCCCATA 
AGGCATAGGC CAAGACCATA CCCGCCGAAT GTAGGTGAGG AAATCCAAAT TGGTCACATC 
CCCAGGGAAG ATGTAGACTA TCACCTGTAC CCACACGGTC CGGGACTCAA TCCAAATGCC 
TCTACAGGAC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGGCCCCATT CCAGGACACT 
TCTGAGTACA TCATTTCATG TCATCCTGTT GGCACTGATG AAGAACCCTT ACAGTTCAGG 
GTTCCTGGAA CTTCTACCAG TGCCACTCTG ACAGGCCTCA CCAGAGGTGC CACCTACAAC 
ATCATAGTGG AGGCACTGAA AGACCAGCAG AGGCATAAGG TTCGGGAAGA GGTTGTTACC 
GTGGGCAACT CTGTCAACGA AGGCTTGAAC CAACCTACGG ATGACTCGTG CTTTGACCCC 
TACACAGTTT CCCATTATGC CGTTGGAGAT GAGTGGGAAC GAATGTCTGA ATCAGGCTTT 
AAACTGTTGT GCCAGTGCTT AGGCTTTGGA AGTGGTCATT TCAGATGTGA TTCATCTAGA 
TGGTGCCATG ACAATGGTGT GAACTACAAG ATTGGAGAGA AGTGGGACCG TCAGGGAGAA 
AATGGCCAGA TGATGAGCTG CACATGTCTT GGGAACGGAA AAGGAGAATT CAAGTGTGAC 
CCTCATGAGG CAACGTGTTA TGATGATGGG AAGACATACC ACGTAGGAGA ACAGTGGCAG 
AAGGAATATC TCGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC 
TGTGACAACT GCCGCAGACC TGGGGGTGAA CCCAGTCCCG AAGGCACTAC TGGCCAGTCC 
TACAACCAGT ATTCTCAGAG ATACCATCAG AGAACAAACA CTAATGTTAA TTGCCCAATT 
GAGTGCTTCA TGCCTTTAGA TGTACAGGCT GACAGAGAAG ATTCCCGAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( NM_212482.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_997647.1 Homo sapiens fibronectin 1 (FN1), transcript variant 1, mRNA.

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CloneID	OHu24787
Clone ID Related Accession (Same CDS sequence)	NM_212482.3 , NM_212482.1 , NM_212482.2
Accession Version	NM_212482.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	fibronectin isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX640803.1, AJ535086.1, BX640875.1, AI033037.1, U60068.1, BX640999.1, BQ005645.1 and CR749317.1. This sequence is a reference standard in the RefSeqGene project. On Jan 9, 2016 this sequence version replaced NM_212482.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). The encoded isoform (1) may undergo proteolytic processing similar to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX640803.1, BX640875.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT 
CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT 
CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA 
CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA 
GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT 
CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT 
ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT 
CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT 
GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT 
GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA 
CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA 
AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA 
ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA 
ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT 
GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG 
CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG 
GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG 
TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC 
ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC 
CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA 
GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC 
ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC 
ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT 
CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC 
ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT 
GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG 
CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG 
ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT 
CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT 
ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA 
AAGACAGACG AGCTTCCCCA ACTGGTAACC CTTCCACACC CCAATCTTCA TGGACCAGAG 
ATCTTGGATG TTCCTTCCAC AGTTCAAAAG ACCCCTTTCG TCACCCACCC TGGGTATGAC 
ACTGGAAATG GTATTCAGCT TCCTGGCACT TCTGGTCAGC AACCCAGTGT TGGGCAACAA 
ATGATCTTTG AGGAACATGG TTTTAGGCGG ACCACACCGC CCACAACGGC CACCCCCATA 
AGGCATAGGC CAAGACCATA CCCGCCGAAT GTAGGTGAGG AAATCCAAAT TGGTCACATC 
CCCAGGGAAG ATGTAGACTA TCACCTGTAC CCACACGGTC CGGGACTCAA TCCAAATGCC 
TCTACAGGAC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGGCCCCATT CCAGGACACT 
TCTGAGTACA TCATTTCATG TCATCCTGTT GGCACTGATG AAGAACCCTT ACAGTTCAGG 
GTTCCTGGAA CTTCTACCAG TGCCACTCTG ACAGGCCTCA CCAGAGGTGC CACCTACAAC 
ATCATAGTGG AGGCACTGAA AGACCAGCAG AGGCATAAGG TTCGGGAAGA GGTTGTTACC 
GTGGGCAACT CTGTCAACGA AGGCTTGAAC CAACCTACGG ATGACTCGTG CTTTGACCCC 
TACACAGTTT CCCATTATGC CGTTGGAGAT GAGTGGGAAC GAATGTCTGA ATCAGGCTTT 
AAACTGTTGT GCCAGTGCTT AGGCTTTGGA AGTGGTCATT TCAGATGTGA TTCATCTAGA 
TGGTGCCATG ACAATGGTGT GAACTACAAG ATTGGAGAGA AGTGGGACCG TCAGGGAGAA 
AATGGCCAGA TGATGAGCTG CACATGTCTT GGGAACGGAA AAGGAGAATT CAAGTGTGAC 
CCTCATGAGG CAACGTGTTA TGATGATGGG AAGACATACC ACGTAGGAGA ACAGTGGCAG 
AAGGAATATC TCGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC 
TGTGACAACT GCCGCAGACC TGGGGGTGAA CCCAGTCCCG AAGGCACTAC TGGCCAGTCC 
TACAACCAGT ATTCTCAGAG ATACCATCAG AGAACAAACA CTAATGTTAA TTGCCCAATT 
GAGTGCTTCA TGCCTTTAGA TGTACAGGCT GACAGAGAAG ATTCCCGAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997647.1
CDS	267..7700
Translation	MLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDPTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSGESQPLVQTAVTNIDRPKGLAFTDVDVDSIKIAWESPQGQVSRYRVTYSSPEDGIHELFPAPDGEEDTAELQGLRPGSEYTVSVVALHDDMESQPLIGTQSTAIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPSTVQKTPFVTHPGYDTGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPIRHRPRPYPPNVGEEIQIGHIPREDVDYHLYPHGPGLNPNASTGQEALSQTTISWAPFQDTSEYIISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGATYNIIVEALKDQQRHKVREEVVTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWERMSESGFKLLCQCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVNCPIECFMPLDVQADREDSRE

Target ORF information:

RefSeq Version	NM_212482.2
Organism	Homo sapiens(human)
Definition	Homo sapiens fibronectin 1 (FN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212482.2

ORF Insert Sequence:

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT 
CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT 
CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA 
CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA 
GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT 
CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT 
ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT 
CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT 
GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT 
GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA 
CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA 
AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA 
ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA 
ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT 
GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG 
CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG 
GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG 
TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC 
ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC 
CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA 
GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC 
ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC 
ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT 
CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC 
ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT 
GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG 
CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG 
ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT 
CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT 
ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA 
AAGACAGACG AGCTTCCCCA ACTGGTAACC CTTCCACACC CCAATCTTCA TGGACCAGAG 
ATCTTGGATG TTCCTTCCAC AGTTCAAAAG ACCCCTTTCG TCACCCACCC TGGGTATGAC 
ACTGGAAATG GTATTCAGCT TCCTGGCACT TCTGGTCAGC AACCCAGTGT TGGGCAACAA 
ATGATCTTTG AGGAACATGG TTTTAGGCGG ACCACACCGC CCACAACGGC CACCCCCATA 
AGGCATAGGC CAAGACCATA CCCGCCGAAT GTAGGTGAGG AAATCCAAAT TGGTCACATC 
CCCAGGGAAG ATGTAGACTA TCACCTGTAC CCACACGGTC CGGGACTCAA TCCAAATGCC 
TCTACAGGAC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGGCCCCATT CCAGGACACT 
TCTGAGTACA TCATTTCATG TCATCCTGTT GGCACTGATG AAGAACCCTT ACAGTTCAGG 
GTTCCTGGAA CTTCTACCAG TGCCACTCTG ACAGGCCTCA CCAGAGGTGC CACCTACAAC 
ATCATAGTGG AGGCACTGAA AGACCAGCAG AGGCATAAGG TTCGGGAAGA GGTTGTTACC 
GTGGGCAACT CTGTCAACGA AGGCTTGAAC CAACCTACGG ATGACTCGTG CTTTGACCCC 
TACACAGTTT CCCATTATGC CGTTGGAGAT GAGTGGGAAC GAATGTCTGA ATCAGGCTTT 
AAACTGTTGT GCCAGTGCTT AGGCTTTGGA AGTGGTCATT TCAGATGTGA TTCATCTAGA 
TGGTGCCATG ACAATGGTGT GAACTACAAG ATTGGAGAGA AGTGGGACCG TCAGGGAGAA 
AATGGCCAGA TGATGAGCTG CACATGTCTT GGGAACGGAA AAGGAGAATT CAAGTGTGAC 
CCTCATGAGG CAACGTGTTA TGATGATGGG AAGACATACC ACGTAGGAGA ACAGTGGCAG 
AAGGAATATC TCGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC 
TGTGACAACT GCCGCAGACC TGGGGGTGAA CCCAGTCCCG AAGGCACTAC TGGCCAGTCC 
TACAACCAGT ATTCTCAGAG ATACCATCAG AGAACAAACA CTAATGTTAA TTGCCCAATT 
GAGTGCTTCA TGCCTTTAGA TGTACAGGCT GACAGAGAAG ATTCCCGAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( NM_054034.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_473375.2 Homo sapiens fibronectin 1 (FN1), transcript variant 7, mRNA.

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CloneID	OHu24809
Clone ID Related Accession (Same CDS sequence)	NM_054034.2
Accession Version	NM_054034.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	fibronectin isoform 7 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX640608.1, AJ276395.1 and AJ535086.1. On May 12, 2004 this sequence version replaced NM_054034.1. Transcript Variant: This variant (7) lacks multiple 3' exons and has an alternate 3' end, as compared to variant 1. Isoform 7 encoded by this variant is much shorter and has a distinct C-terminus, as compared isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ535086.1, BX538045.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467147, SAMEA2467148 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TGTGAGTATC CCACCCAGAA ACCTTGGATA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_473375.2
CDS	267..2240
Translation	MLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPVSIPPRNLGY

Target ORF information:

RefSeq Version	NM_054034.2
Organism	Homo sapiens(human)
Definition	Homo sapiens fibronectin 1 (FN1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_054034.2

ORF Insert Sequence:

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TGTGAGTATC CCACCCAGAA ACCTTGGATA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( NM_001365519.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001352448.1 Homo sapiens fibronectin 1 (FN1), transcript variant 14, mRNA.

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CloneID	OHu107507
Clone ID Related Accession (Same CDS sequence)	NM_001365519.1
Accession Version	NM_001365519.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	fibronectin isoform 14 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012462.5 and AC073284.5. On Aug 30, 2018 this sequence version replaced XM_024452771.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.52979.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT 
CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT 
CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA 
CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA 
GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT 
CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT 
ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT 
CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT 
GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT 
GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA 
CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA 
AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA 
ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC 
GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG 
GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA 
TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG 
ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG 
GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG 
ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA 
ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC 
AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC 
TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG 
CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG 
CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT 
ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT 
CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGTTC AAAAGACCCC TTTCGTCACC 
CACCCTGGGT ATGACACTGG AAATGGTATT CAGCTTCCTG GCACTTCTGG TCAGCAACCC 
AGTGTTGGGC AACAAATGAT CTTTGAGGAA CATGGTTTTA GGCGGACCAC ACCGCCCACA 
ACGGCCACCC CCATAAGGCA TAGGCCAAGA CCATACCCGC CGAATGTAGG TGAGGAAATC 
CAAATTGGTC ACATCCCCAG GGAAGATGTA GACTATCACC TGTACCCACA CGGTCCGGGA 
CTCAATCCAA ATGCCTCTAC AGGACAAGAA GCTCTCTCTC AGACAACCAT CTCATGGGCC 
CCATTCCAGG ACACTTCTGA GTACATCATT TCATGTCATC CTGTTGGCAC TGATGAAGAA 
CCCTTACAGT TCAGGGTTCC TGGAACTTCT ACCAGTGCCA CTCTGACAGG CCTCACCAGA 
GGTGCCACCT ACAACGTCAT AGTGGAGGCA CTGAAAGACC AGCAGAGGCA TAAGGTTCGG 
GAAGAGGTTG TTACCGTGGG CAACTCTGTC AACGAAGGCT TGAACCAACC TACGGATGAC 
TCGTGCTTTG ACCCCTACAC AGTTTCCCAT TATGCCGTTG GAGATGAGTG GGAACGAATG 
TCTGAATCAG GCTTTAAACT GTTGTGCCAG TGCTTAGGCT TTGGAAGTGG TCATTTCAGA 
TGTGATTCAT CTAGATGGTG CCATGACAAT GGTGTGAACT ACAAGATTGG AGAGAAGTGG 
GACCGTCAGG GAGAAAATGG CCAGATGATG AGCTGCACAT GTCTTGGGAA CGGAAAAGGA 
GAATTCAAGT GTGACCCTCA TGAGGCAACG TGTTATGATG ATGGGAAGAC ATACCACGTA 
GGAGAACAGT GGCAGAAGGA ATATCTCGGT GCCATTTGCT CCTGCACATG CTTTGGAGGC 
CAGCGGGGCT GGCGCTGTGA CAACTGCCGC AGACCTGGGG GTGAACCCAG TCCCGAAGGC 
ACTACTGGCC AGTCCTACAA CCAGTATTCT CAGAGATACC ATCAGAGAAC AAACACTAAT 
GTTAATTGCC CAATTGAGTG CTTCATGCCT TTAGATGTAC AGGCTGACAG AGAAGATTCC 
CGAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352448.1
CDS	267..7355
Translation	MLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSGESQPLVQTAVTTIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTVQKTPFVTHPGYDTGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPIRHRPRPYPPNVGEEIQIGHIPREDVDYHLYPHGPGLNPNASTGQEALSQTTISWAPFQDTSEYIISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGATYNVIVEALKDQQRHKVREEVVTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWERMSESGFKLLCQCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVNCPIECFMPLDVQADREDSRE

Target ORF information:

RefSeq Version	NM_001365519.1
Organism	Homo sapiens(human)
Definition	Homo sapiens fibronectin 1 (FN1), transcript variant 14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365519.1

ORF Insert Sequence:

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT 
CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT 
CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA 
CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA 
GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT 
CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT 
ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT 
CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT 
GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT 
GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA 
CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA 
AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA 
ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC 
GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG 
GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA 
TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG 
ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG 
GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG 
ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA 
ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC 
AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC 
TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG 
CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG 
CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT 
ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT 
CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGTTC AAAAGACCCC TTTCGTCACC 
CACCCTGGGT ATGACACTGG AAATGGTATT CAGCTTCCTG GCACTTCTGG TCAGCAACCC 
AGTGTTGGGC AACAAATGAT CTTTGAGGAA CATGGTTTTA GGCGGACCAC ACCGCCCACA 
ACGGCCACCC CCATAAGGCA TAGGCCAAGA CCATACCCGC CGAATGTAGG TGAGGAAATC 
CAAATTGGTC ACATCCCCAG GGAAGATGTA GACTATCACC TGTACCCACA CGGTCCGGGA 
CTCAATCCAA ATGCCTCTAC AGGACAAGAA GCTCTCTCTC AGACAACCAT CTCATGGGCC 
CCATTCCAGG ACACTTCTGA GTACATCATT TCATGTCATC CTGTTGGCAC TGATGAAGAA 
CCCTTACAGT TCAGGGTTCC TGGAACTTCT ACCAGTGCCA CTCTGACAGG CCTCACCAGA 
GGTGCCACCT ACAACGTCAT AGTGGAGGCA CTGAAAGACC AGCAGAGGCA TAAGGTTCGG 
GAAGAGGTTG TTACCGTGGG CAACTCTGTC AACGAAGGCT TGAACCAACC TACGGATGAC 
TCGTGCTTTG ACCCCTACAC AGTTTCCCAT TATGCCGTTG GAGATGAGTG GGAACGAATG 
TCTGAATCAG GCTTTAAACT GTTGTGCCAG TGCTTAGGCT TTGGAAGTGG TCATTTCAGA 
TGTGATTCAT CTAGATGGTG CCATGACAAT GGTGTGAACT ACAAGATTGG AGAGAAGTGG 
GACCGTCAGG GAGAAAATGG CCAGATGATG AGCTGCACAT GTCTTGGGAA CGGAAAAGGA 
GAATTCAAGT GTGACCCTCA TGAGGCAACG TGTTATGATG ATGGGAAGAC ATACCACGTA 
GGAGAACAGT GGCAGAAGGA ATATCTCGGT GCCATTTGCT CCTGCACATG CTTTGGAGGC 
CAGCGGGGCT GGCGCTGTGA CAACTGCCGC AGACCTGGGG GTGAACCCAG TCCCGAAGGC 
ACTACTGGCC AGTCCTACAA CCAGTATTCT CAGAGATACC ATCAGAGAAC AAACACTAAT 
GTTAATTGCC CAATTGAGTG CTTCATGCCT TTAGATGTAC AGGCTGACAG AGAAGATTCC 
CGAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( NM_001365521.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001352450.1 Homo sapiens fibronectin 1 (FN1), transcript variant 16, mRNA.

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CloneID	OHu89216
Clone ID Related Accession (Same CDS sequence)	XM_017003693.1 , NM_001365521.1
Accession Version	NM_001365521.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Homo sapiens(human)
Product	fibronectin isoform 16 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012462.5 and AC073284.5. On Aug 30, 2018 this sequence version replaced XM_017003693.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.226196.1, SRR1803613.233494.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT 
CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT 
CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA 
CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA 
GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT 
CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT 
ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT 
CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT 
GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT 
GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA 
CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA 
AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA 
ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC 
GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG 
GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA 
TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG 
ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG 
GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG 
ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA 
ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC 
AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC 
TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG 
CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG 
CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT 
ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT 
CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGACG AGCTTCCCCA ACTGGTAACC 
CTTCCACACC CCAATCTTCA TGGACCAGAG ATCTTGGATG TTCCTTCCAC AGTTCAAAAG 
ACCCCTTTCG TCACCCACCC TGGGTATGAC ACTGGAAATG GTATTCAGCT TCCTGGCACT 
TCTGGTCAGC AACCCAGTGT TGGGCAACAA ATGATCTTTG AGGAACATGG TTTTAGGCGG 
ACCACACCGC CCACAACGGC CACCCCCATA AGGCATAGGC CAAGACCATA CCCGCCGAAT 
GTAGGACAAG AAGCTCTCTC TCAGACAACC ATCTCATGGG CCCCATTCCA GGACACTTCT 
GAGTACATCA TTTCATGTCA TCCTGTTGGC ACTGATGAAG AACCCTTACA GTTCAGGGTT 
CCTGGAACTT CTACCAGTGC CACTCTGACA GGCCTCACCA GAGGTGCCAC CTACAACGTC 
ATAGTGGAGG CACTGAAAGA CCAGCAGAGG CATAAGGTTC GGGAAGAGGT TGTTACCGTG 
GGCAACTCTG TCAACGAAGG CTTGAACCAA CCTACGGATG ACTCGTGCTT TGACCCCTAC 
ACAGTTTCCC ATTATGCCGT TGGAGATGAG TGGGAACGAA TGTCTGAATC AGGCTTTAAA 
CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT GGTCATTTCA GATGTGATTC ATCTAGATGG 
TGCCATGACA ATGGTGTGAA CTACAAGATT GGAGAGAAGT GGGACCGTCA GGGAGAAAAT 
GGCCAGATGA TGAGCTGCAC ATGTCTTGGG AACGGAAAAG GAGAATTCAA GTGTGACCCT 
CATGAGGCAA CGTGTTATGA TGATGGGAAG ACATACCACG TAGGAGAACA GTGGCAGAAG 
GAATATCTCG GTGCCATTTG CTCCTGCACA TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT 
GACAACTGCC GCAGACCTGG GGGTGAACCC AGTCCCGAAG GCACTACTGG CCAGTCCTAC 
AACCAGTATT CTCAGAGATA CCATCAGAGA ACAAACACTA ATGTTAATTG CCCAATTGAG 
TGCTTCATGC CTTTAGATGT ACAGGCTGAC AGAGAAGATT CCCGAGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352450.1
CDS	267..7337
Translation	MLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSGESQPLVQTAVTTIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPSTVQKTPFVTHPGYDTGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPIRHRPRPYPPNVGQEALSQTTISWAPFQDTSEYIISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGATYNVIVEALKDQQRHKVREEVVTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWERMSESGFKLLCQCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVNCPIECFMPLDVQADREDSRE

Target ORF information:

RefSeq Version	NM_001365521.1
Organism	Homo sapiens(human)
Definition	Homo sapiens fibronectin 1 (FN1), transcript variant 16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365521.1

ORF Insert Sequence:

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT 
CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT 
CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA 
CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA 
GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT 
CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT 
ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT 
CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT 
GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT 
GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA 
CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA 
AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA 
ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC 
GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG 
GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA 
TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG 
ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG 
GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG 
ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA 
ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC 
AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC 
TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG 
CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG 
CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT 
ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT 
CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGACG AGCTTCCCCA ACTGGTAACC 
CTTCCACACC CCAATCTTCA TGGACCAGAG ATCTTGGATG TTCCTTCCAC AGTTCAAAAG 
ACCCCTTTCG TCACCCACCC TGGGTATGAC ACTGGAAATG GTATTCAGCT TCCTGGCACT 
TCTGGTCAGC AACCCAGTGT TGGGCAACAA ATGATCTTTG AGGAACATGG TTTTAGGCGG 
ACCACACCGC CCACAACGGC CACCCCCATA AGGCATAGGC CAAGACCATA CCCGCCGAAT 
GTAGGACAAG AAGCTCTCTC TCAGACAACC ATCTCATGGG CCCCATTCCA GGACACTTCT 
GAGTACATCA TTTCATGTCA TCCTGTTGGC ACTGATGAAG AACCCTTACA GTTCAGGGTT 
CCTGGAACTT CTACCAGTGC CACTCTGACA GGCCTCACCA GAGGTGCCAC CTACAACGTC 
ATAGTGGAGG CACTGAAAGA CCAGCAGAGG CATAAGGTTC GGGAAGAGGT TGTTACCGTG 
GGCAACTCTG TCAACGAAGG CTTGAACCAA CCTACGGATG ACTCGTGCTT TGACCCCTAC 
ACAGTTTCCC ATTATGCCGT TGGAGATGAG TGGGAACGAA TGTCTGAATC AGGCTTTAAA 
CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT GGTCATTTCA GATGTGATTC ATCTAGATGG 
TGCCATGACA ATGGTGTGAA CTACAAGATT GGAGAGAAGT GGGACCGTCA GGGAGAAAAT 
GGCCAGATGA TGAGCTGCAC ATGTCTTGGG AACGGAAAAG GAGAATTCAA GTGTGACCCT 
CATGAGGCAA CGTGTTATGA TGATGGGAAG ACATACCACG TAGGAGAACA GTGGCAGAAG 
GAATATCTCG GTGCCATTTG CTCCTGCACA TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT 
GACAACTGCC GCAGACCTGG GGGTGAACCC AGTCCCGAAG GCACTACTGG CCAGTCCTAC 
AACCAGTATT CTCAGAGATA CCATCAGAGA ACAAACACTA ATGTTAATTG CCCAATTGAG 
TGCTTCATGC CTTTAGATGT ACAGGCTGAC AGAGAAGATT CCCGAGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( NM_001365522.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001352451.1 Homo sapiens fibronectin 1 (FN1), transcript variant 17, mRNA.

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CloneID	OHu89217
Clone ID Related Accession (Same CDS sequence)	NM_001365522.1 , XM_017003694.1
Accession Version	NM_001365522.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	fibronectin isoform 17 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012462.5 and AC073284.5. On Aug 30, 2018 this sequence version replaced XM_017003694.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.14796.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT 
CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT 
CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA 
CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA 
GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT 
CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT 
ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT 
CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT 
GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT 
GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA 
CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA 
AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA 
ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC 
GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG 
GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA 
TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG 
ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG 
GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG 
ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA 
ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC 
AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC 
TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG 
CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG 
CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT 
ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT 
CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGTTC AAAAGACCCC TTTCGTCACC 
CACCCTGGGT ATGACACTGG AAATGGTATT CAGCTTCCTG GCACTTCTGG TCAGCAACCC 
AGTGTTGGGC AACAAATGAT CTTTGAGGAA CATGGTTTTA GGCGGACCAC ACCGCCCACA 
ACGGCCACCC CCATAAGGCA TAGGCCAAGA CCATACCCGC CGAATGTAGG ACAAGAAGCT 
CTCTCTCAGA CAACCATCTC ATGGGCCCCA TTCCAGGACA CTTCTGAGTA CATCATTTCA 
TGTCATCCTG TTGGCACTGA TGAAGAACCC TTACAGTTCA GGGTTCCTGG AACTTCTACC 
AGTGCCACTC TGACAGGCCT CACCAGAGGT GCCACCTACA ACGTCATAGT GGAGGCACTG 
AAAGACCAGC AGAGGCATAA GGTTCGGGAA GAGGTTGTTA CCGTGGGCAA CTCTGTCAAC 
GAAGGCTTGA ACCAACCTAC GGATGACTCG TGCTTTGACC CCTACACAGT TTCCCATTAT 
GCCGTTGGAG ATGAGTGGGA ACGAATGTCT GAATCAGGCT TTAAACTGTT GTGCCAGTGC 
TTAGGCTTTG GAAGTGGTCA TTTCAGATGT GATTCATCTA GATGGTGCCA TGACAATGGT 
GTGAACTACA AGATTGGAGA GAAGTGGGAC CGTCAGGGAG AAAATGGCCA GATGATGAGC 
TGCACATGTC TTGGGAACGG AAAAGGAGAA TTCAAGTGTG ACCCTCATGA GGCAACGTGT 
TATGATGATG GGAAGACATA CCACGTAGGA GAACAGTGGC AGAAGGAATA TCTCGGTGCC 
ATTTGCTCCT GCACATGCTT TGGAGGCCAG CGGGGCTGGC GCTGTGACAA CTGCCGCAGA 
CCTGGGGGTG AACCCAGTCC CGAAGGCACT ACTGGCCAGT CCTACAACCA GTATTCTCAG 
AGATACCATC AGAGAACAAA CACTAATGTT AATTGCCCAA TTGAGTGCTT CATGCCTTTA 
GATGTACAGG CTGACAGAGA AGATTCCCGA GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352451.1
CDS	267..7262
Translation	MLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSGESQPLVQTAVTTIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTVQKTPFVTHPGYDTGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPIRHRPRPYPPNVGQEALSQTTISWAPFQDTSEYIISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGATYNVIVEALKDQQRHKVREEVVTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWERMSESGFKLLCQCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVNCPIECFMPLDVQADREDSRE

Target ORF information:

RefSeq Version	NM_001365522.1
Organism	Homo sapiens(human)
Definition	Homo sapiens fibronectin 1 (FN1), transcript variant 17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365522.1

ORF Insert Sequence:

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT 
CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT 
CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA 
CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA 
GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT 
CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT 
ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT 
CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT 
GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT 
GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA 
CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA 
AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA 
ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC 
GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG 
GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA 
TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG 
ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG 
GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG 
ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA 
ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC 
AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC 
TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG 
CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG 
CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT 
ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT 
CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGTTC AAAAGACCCC TTTCGTCACC 
CACCCTGGGT ATGACACTGG AAATGGTATT CAGCTTCCTG GCACTTCTGG TCAGCAACCC 
AGTGTTGGGC AACAAATGAT CTTTGAGGAA CATGGTTTTA GGCGGACCAC ACCGCCCACA 
ACGGCCACCC CCATAAGGCA TAGGCCAAGA CCATACCCGC CGAATGTAGG ACAAGAAGCT 
CTCTCTCAGA CAACCATCTC ATGGGCCCCA TTCCAGGACA CTTCTGAGTA CATCATTTCA 
TGTCATCCTG TTGGCACTGA TGAAGAACCC TTACAGTTCA GGGTTCCTGG AACTTCTACC 
AGTGCCACTC TGACAGGCCT CACCAGAGGT GCCACCTACA ACGTCATAGT GGAGGCACTG 
AAAGACCAGC AGAGGCATAA GGTTCGGGAA GAGGTTGTTA CCGTGGGCAA CTCTGTCAAC 
GAAGGCTTGA ACCAACCTAC GGATGACTCG TGCTTTGACC CCTACACAGT TTCCCATTAT 
GCCGTTGGAG ATGAGTGGGA ACGAATGTCT GAATCAGGCT TTAAACTGTT GTGCCAGTGC 
TTAGGCTTTG GAAGTGGTCA TTTCAGATGT GATTCATCTA GATGGTGCCA TGACAATGGT 
GTGAACTACA AGATTGGAGA GAAGTGGGAC CGTCAGGGAG AAAATGGCCA GATGATGAGC 
TGCACATGTC TTGGGAACGG AAAAGGAGAA TTCAAGTGTG ACCCTCATGA GGCAACGTGT 
TATGATGATG GGAAGACATA CCACGTAGGA GAACAGTGGC AGAAGGAATA TCTCGGTGCC 
ATTTGCTCCT GCACATGCTT TGGAGGCCAG CGGGGCTGGC GCTGTGACAA CTGCCGCAGA 
CCTGGGGGTG AACCCAGTCC CGAAGGCACT ACTGGCCAGT CCTACAACCA GTATTCTCAG 
AGATACCATC AGAGAACAAA CACTAATGTT AATTGCCCAA TTGAGTGCTT CATGCCTTTA 
GATGTACAGG CTGACAGAGA AGATTCCCGA GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( XM_024452769.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308537.1 Homo sapiens fibronectin 1 (FN1), transcript variant X4, mRNA.

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CloneID	OHu104833
Clone ID Related Accession (Same CDS sequence)	XM_024452769.1
Accession Version	XM_024452769.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	fibronectin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT 
CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT 
CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA 
CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA 
GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT 
CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT 
ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT 
CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT 
GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT 
GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA 
CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA 
AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA 
ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA 
ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT 
GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG 
CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG 
GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG 
TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC 
ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC 
CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA 
GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC 
ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC 
ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT 
CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC 
ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT 
GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG 
CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG 
ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT 
CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT 
ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA 
AAGACAGTTC AAAAGACCCC TTTCGTCACC CACCCTGGGT ATGACACTGG AAATGGTATT 
CAGCTTCCTG GCACTTCTGG TCAGCAACCC AGTGTTGGGC AACAAATGAT CTTTGAGGAA 
CATGGTTTTA GGCGGACCAC ACCGCCCACA ACGGCCACCC CCATAAGGCA TAGGCCAAGA 
CCATACCCGC CGAATGTAGG TGAGGAAATC CAAATTGGTC ACATCCCCAG GGAAGATGTA 
GACTATCACC TGTACCCACA CGGTCCGGGA CTCAATCCAA ATGCCTCTAC AGGACAAGAA 
GCTCTCTCTC AGACAACCAT CTCATGGGCC CCATTCCAGG ACACTTCTGA GTACATCATT 
TCATGTCATC CTGTTGGCAC TGATGAAGAA CCCTTACAGT TCAGGGTTCC TGGAACTTCT 
ACCAGTGCCA CTCTGACAGG CCTCACCAGA GGTGCCACCT ACAACGTCAT AGTGGAGGCA 
CTGAAAGACC AGCAGAGGCA TAAGGTTCGG GAAGAGGTTG TTACCGTGGG CAACTCTGTC 
AACGAAGGCT TGAACCAACC TACGGATGAC TCGTGCTTTG ACCCCTACAC AGTTTCCCAT 
TATGCCGTTG GAGATGAGTG GGAACGAATG TCTGAATCAG GCTTTAAACT GTTGTGCCAG 
TGCTTAGGCT TTGGAAGTGG TCATTTCAGA TGTGATTCAT CTAGATGGTG CCATGACAAT 
GGTGTGAACT ACAAGATTGG AGAGAAGTGG GACCGTCAGG GAGAAAATGG CCAGATGATG 
AGCTGCACAT GTCTTGGGAA CGGAAAAGGA GAATTCAAGT GTGACCCTCA TGAGGCAACG 
TGTTATGATG ATGGGAAGAC ATACCACGTA GGAGAACAGT GGCAGAAGGA ATATCTCGGT 
GCCATTTGCT CCTGCACATG CTTTGGAGGC CAGCGGGGCT GGCGCTGTGA CAACTGCCGC 
AGACCTGGGG GTGAACCCAG TCCCGAAGGC ACTACTGGCC AGTCCTACAA CCAGTATTCT 
CAGAGATACC ATCAGAGAAC AAACACTAAT GTTAATTGCC CAATTGAGTG CTTCATGCCT 
TTAGATGTAC AGGCTGACAG AGAAGATTCC CGAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308537.1
CDS	366..7724
Translation	MLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSGESQPLVQTAVTNIDRPKGLAFTDVDVDSIKIAWESPQGQVSRYRVTYSSPEDGIHELFPAPDGEEDTAELQGLRPGSEYTVSVVALHDDMESQPLIGTQSTAIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTVQKTPFVTHPGYDTGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPIRHRPRPYPPNVGEEIQIGHIPREDVDYHLYPHGPGLNPNASTGQEALSQTTISWAPFQDTSEYIISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGATYNVIVEALKDQQRHKVREEVVTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWERMSESGFKLLCQCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVNCPIECFMPLDVQADREDSRE

Target ORF information:

RefSeq Version	XM_024452769.1
Organism	Homo sapiens(human)
Definition	Homo sapiens fibronectin 1 (FN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452769.1

ORF Insert Sequence:

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT 
CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT 
CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA 
CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA 
GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT 
CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT 
ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT 
CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT 
GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT 
GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA 
CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA 
AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA 
ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA 
ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT 
GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG 
CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG 
GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG 
TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC 
ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC 
CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA 
GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC 
ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC 
ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT 
CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC 
ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT 
GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG 
CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG 
ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT 
CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT 
ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA 
AAGACAGTTC AAAAGACCCC TTTCGTCACC CACCCTGGGT ATGACACTGG AAATGGTATT 
CAGCTTCCTG GCACTTCTGG TCAGCAACCC AGTGTTGGGC AACAAATGAT CTTTGAGGAA 
CATGGTTTTA GGCGGACCAC ACCGCCCACA ACGGCCACCC CCATAAGGCA TAGGCCAAGA 
CCATACCCGC CGAATGTAGG TGAGGAAATC CAAATTGGTC ACATCCCCAG GGAAGATGTA 
GACTATCACC TGTACCCACA CGGTCCGGGA CTCAATCCAA ATGCCTCTAC AGGACAAGAA 
GCTCTCTCTC AGACAACCAT CTCATGGGCC CCATTCCAGG ACACTTCTGA GTACATCATT 
TCATGTCATC CTGTTGGCAC TGATGAAGAA CCCTTACAGT TCAGGGTTCC TGGAACTTCT 
ACCAGTGCCA CTCTGACAGG CCTCACCAGA GGTGCCACCT ACAACGTCAT AGTGGAGGCA 
CTGAAAGACC AGCAGAGGCA TAAGGTTCGG GAAGAGGTTG TTACCGTGGG CAACTCTGTC 
AACGAAGGCT TGAACCAACC TACGGATGAC TCGTGCTTTG ACCCCTACAC AGTTTCCCAT 
TATGCCGTTG GAGATGAGTG GGAACGAATG TCTGAATCAG GCTTTAAACT GTTGTGCCAG 
TGCTTAGGCT TTGGAAGTGG TCATTTCAGA TGTGATTCAT CTAGATGGTG CCATGACAAT 
GGTGTGAACT ACAAGATTGG AGAGAAGTGG GACCGTCAGG GAGAAAATGG CCAGATGATG 
AGCTGCACAT GTCTTGGGAA CGGAAAAGGA GAATTCAAGT GTGACCCTCA TGAGGCAACG 
TGTTATGATG ATGGGAAGAC ATACCACGTA GGAGAACAGT GGCAGAAGGA ATATCTCGGT 
GCCATTTGCT CCTGCACATG CTTTGGAGGC CAGCGGGGCT GGCGCTGTGA CAACTGCCGC 
AGACCTGGGG GTGAACCCAG TCCCGAAGGC ACTACTGGCC AGTCCTACAA CCAGTATTCT 
CAGAGATACC ATCAGAGAAC AAACACTAAT GTTAATTGCC CAATTGAGTG CTTCATGCCT 
TTAGATGTAC AGGCTGACAG AGAAGATTCC CGAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( XM_024452770.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308538.1 Homo sapiens fibronectin 1 (FN1), transcript variant X11, mRNA.

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CloneID	OHu104834
Clone ID Related Accession (Same CDS sequence)	XM_024452770.1
Accession Version	XM_024452770.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	fibronectin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT 
CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT 
CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA 
CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA 
GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT 
CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT 
ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT 
CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT 
GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT 
GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA 
CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA 
AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA 
ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC 
GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG 
GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA 
TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG 
ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG 
GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG 
ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA 
ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC 
AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC 
TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG 
CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG 
CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT 
ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT 
CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGTTC AAAAGACCCC TTTCGTCACC 
CACCCTGGGT ATGACACTGG AAATGGTATT CAGCTTCCTG GCACTTCTGG TCAGCAACCC 
AGTGTTGGGC AACAAATGAT CTTTGAGGAA CATGGTTTTA GGCGGACCAC ACCGCCCACA 
ACGGCCACCC CCATAAGGCA TAGGCCAAGA CCATACCCGC CGAATGTAGG TGAGGAAATC 
CAAATTGGTC ACATCCCCAG GGAAGATGTA GACTATCACC TGTACCCACA CGGTCCGGGA 
CTCAATCCAA ATGCCTCTAC AGGACAAGAA GCTCTCTCTC AGACAACCAT CTCATGGGCC 
CCATTCCAGG ACACTTCTGA GTACATCATT TCATGTCATC CTGTTGGCAC TGATGAAGAA 
CCCTTACAGT TCAGGGTTCC TGGAACTTCT ACCAGTGCCA CTCTGACAGG CCTCACCAGA 
GGTGCCACCT ACAACGTCAT AGTGGAGGCA CTGAAAGACC AGCAGAGGCA TAAGGTTCGG 
GAAGAGGTTG TTACCGTGGG CAACTCTGTC AACGAAGGCT TGAACCAACC TACGGATGAC 
TCGTGCTTTG ACCCCTACAC AGTTTCCCAT TATGCCGTTG GAGATGAGTG GGAACGAATG 
TCTGAATCAG GCTTTAAACT GTTGTGCCAG TGCTTAGGCT TTGGAAGTGG TCATTTCAGA 
TGTGATTCAT CTAGATGGTG CCATGACAAT GGTGTGAACT ACAAGATTGG AGAGAAGTGG 
GACCGTCAGG GAGAAAATGG CCAGATGATG AGCTGCACAT GTCTTGGGAA CGGAAAAGGA 
GAATTCAAGT GTGACCCTCA TGAGGCAACG TGTTATGATG ATGGGAAGAC ATACCACGTA 
GGAGAACAGT GGCAGAAGGA ATATCTCGGT GCCATTTGCT CCTGCACATG CTTTGGAGGC 
CAGCGGGGCT GGCGCTGTGA CAACTGCCGC AGACCTGGGG GTGAACCCAG TCCCGAAGGC 
ACTACTGGCC AGTCCTACAA CCAGTATTCT CAGAGATACC ATCAGAGAAC AAACACTGTA 
AGTGCATTAG CAGCACAAGT GTGTTCCCTC ATACTAGACA GTCTCTTTCT ACAGGTATCT 
TTCTTCAGAA TGAACCAAGT GTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308538.1
CDS	366..7472
Translation	MLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSGESQPLVQTAVTTIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTVQKTPFVTHPGYDTGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPIRHRPRPYPPNVGEEIQIGHIPREDVDYHLYPHGPGLNPNASTGQEALSQTTISWAPFQDTSEYIISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGATYNVIVEALKDQQRHKVREEVVTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWERMSESGFKLLCQCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTVSALAAQVCSLILDSLFLQVSFFRMNQVF

Target ORF information:

RefSeq Version	XM_024452770.1
Organism	Homo sapiens(human)
Definition	Homo sapiens fibronectin 1 (FN1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452770.1

ORF Insert Sequence:

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT 
CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT 
CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA 
CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA 
GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT 
CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT 
ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT 
CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT 
GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT 
GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA 
CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA 
AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA 
ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC 
GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG 
GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA 
TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG 
ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG 
GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG 
ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA 
ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC 
AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC 
TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG 
CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG 
CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT 
ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT 
CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGTTC AAAAGACCCC TTTCGTCACC 
CACCCTGGGT ATGACACTGG AAATGGTATT CAGCTTCCTG GCACTTCTGG TCAGCAACCC 
AGTGTTGGGC AACAAATGAT CTTTGAGGAA CATGGTTTTA GGCGGACCAC ACCGCCCACA 
ACGGCCACCC CCATAAGGCA TAGGCCAAGA CCATACCCGC CGAATGTAGG TGAGGAAATC 
CAAATTGGTC ACATCCCCAG GGAAGATGTA GACTATCACC TGTACCCACA CGGTCCGGGA 
CTCAATCCAA ATGCCTCTAC AGGACAAGAA GCTCTCTCTC AGACAACCAT CTCATGGGCC 
CCATTCCAGG ACACTTCTGA GTACATCATT TCATGTCATC CTGTTGGCAC TGATGAAGAA 
CCCTTACAGT TCAGGGTTCC TGGAACTTCT ACCAGTGCCA CTCTGACAGG CCTCACCAGA 
GGTGCCACCT ACAACGTCAT AGTGGAGGCA CTGAAAGACC AGCAGAGGCA TAAGGTTCGG 
GAAGAGGTTG TTACCGTGGG CAACTCTGTC AACGAAGGCT TGAACCAACC TACGGATGAC 
TCGTGCTTTG ACCCCTACAC AGTTTCCCAT TATGCCGTTG GAGATGAGTG GGAACGAATG 
TCTGAATCAG GCTTTAAACT GTTGTGCCAG TGCTTAGGCT TTGGAAGTGG TCATTTCAGA 
TGTGATTCAT CTAGATGGTG CCATGACAAT GGTGTGAACT ACAAGATTGG AGAGAAGTGG 
GACCGTCAGG GAGAAAATGG CCAGATGATG AGCTGCACAT GTCTTGGGAA CGGAAAAGGA 
GAATTCAAGT GTGACCCTCA TGAGGCAACG TGTTATGATG ATGGGAAGAC ATACCACGTA 
GGAGAACAGT GGCAGAAGGA ATATCTCGGT GCCATTTGCT CCTGCACATG CTTTGGAGGC 
CAGCGGGGCT GGCGCTGTGA CAACTGCCGC AGACCTGGGG GTGAACCCAG TCCCGAAGGC 
ACTACTGGCC AGTCCTACAA CCAGTATTCT CAGAGATACC ATCAGAGAAC AAACACTGTA 
AGTGCATTAG CAGCACAAGT GTGTTCCCTC ATACTAGACA GTCTCTTTCT ACAGGTATCT 
TTCTTCAGAA TGAACCAAGT GTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( XM_017003692.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859181.1 Homo sapiens fibronectin 1 (FN1), transcript variant X16, mRNA.

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CloneID	OHu89215
Clone ID Related Accession (Same CDS sequence)	XM_017003692.1
Accession Version	XM_017003692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	fibronectin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT 
CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT 
CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA 
CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA 
GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT 
CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT 
ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT 
CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT 
GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT 
GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA 
CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA 
AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA 
ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA 
ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT 
GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG 
CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG 
GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG 
TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC 
ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC 
CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA 
GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC 
ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC 
ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT 
CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC 
ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT 
GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG 
CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG 
ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT 
CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT 
ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA 
AAGACAGGAC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGGCCCCATT CCAGGACACT 
TCTGAGTACA TCATTTCATG TCATCCTGTT GGCACTGATG AAGAACCCTT ACAGTTCAGG 
GTTCCTGGAA CTTCTACCAG TGCCACTCTG ACAGGCCTCA CCAGAGGTGC CACCTACAAC 
GTCATAGTGG AGGCACTGAA AGACCAGCAG AGGCATAAGG TTCGGGAAGA GGTTGTTACC 
GTGGGCAACT CTGTCAACGA AGGCTTGAAC CAACCTACGG ATGACTCGTG CTTTGACCCC 
TACACAGTTT CCCATTATGC CGTTGGAGAT GAGTGGGAAC GAATGTCTGA ATCAGGCTTT 
AAACTGTTGT GCCAGTGCTT AGGCTTTGGA AGTGGTCATT TCAGATGTGA TTCATCTAGA 
TGGTGCCATG ACAATGGTGT GAACTACAAG ATTGGAGAGA AGTGGGACCG TCAGGGAGAA 
AATGGCCAGA TGATGAGCTG CACATGTCTT GGGAACGGAA AAGGAGAATT CAAGTGTGAC 
CCTCATGAGG CAACGTGTTA TGATGATGGG AAGACATACC ACGTAGGAGA ACAGTGGCAG 
AAGGAATATC TCGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC 
TGTGACAACT GCCGCAGACC TGGGGGTGAA CCCAGTCCCG AAGGCACTAC TGGCCAGTCC 
TACAACCAGT ATTCTCAGAG ATACCATCAG AGAACAAACA CTAATGTTAA TTGCCCAATT 
GAGTGCTTCA TGCCTTTAGA TGTACAGGCT GACAGAGAAG ATTCCCGAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859181.1
CDS	366..7439
Translation	MLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSGESQPLVQTAVTNIDRPKGLAFTDVDVDSIKIAWESPQGQVSRYRVTYSSPEDGIHELFPAPDGEEDTAELQGLRPGSEYTVSVVALHDDMESQPLIGTQSTAIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTGQEALSQTTISWAPFQDTSEYIISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGATYNVIVEALKDQQRHKVREEVVTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWERMSESGFKLLCQCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVNCPIECFMPLDVQADREDSRE

Target ORF information:

RefSeq Version	XM_017003692.1
Organism	Homo sapiens(human)
Definition	Homo sapiens fibronectin 1 (FN1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003692.1

ORF Insert Sequence:

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT 
CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT 
CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA 
CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA 
GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT 
CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT 
ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT 
CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT 
GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT 
GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA 
CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA 
AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA 
ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA 
ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT 
GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG 
CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG 
GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG 
TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC 
ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC 
CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA 
GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC 
ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC 
ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT 
CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC 
ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT 
GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG 
CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG 
ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT 
CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT 
ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA 
AAGACAGGAC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGGCCCCATT CCAGGACACT 
TCTGAGTACA TCATTTCATG TCATCCTGTT GGCACTGATG AAGAACCCTT ACAGTTCAGG 
GTTCCTGGAA CTTCTACCAG TGCCACTCTG ACAGGCCTCA CCAGAGGTGC CACCTACAAC 
GTCATAGTGG AGGCACTGAA AGACCAGCAG AGGCATAAGG TTCGGGAAGA GGTTGTTACC 
GTGGGCAACT CTGTCAACGA AGGCTTGAAC CAACCTACGG ATGACTCGTG CTTTGACCCC 
TACACAGTTT CCCATTATGC CGTTGGAGAT GAGTGGGAAC GAATGTCTGA ATCAGGCTTT 
AAACTGTTGT GCCAGTGCTT AGGCTTTGGA AGTGGTCATT TCAGATGTGA TTCATCTAGA 
TGGTGCCATG ACAATGGTGT GAACTACAAG ATTGGAGAGA AGTGGGACCG TCAGGGAGAA 
AATGGCCAGA TGATGAGCTG CACATGTCTT GGGAACGGAA AAGGAGAATT CAAGTGTGAC 
CCTCATGAGG CAACGTGTTA TGATGATGGG AAGACATACC ACGTAGGAGA ACAGTGGCAG 
AAGGAATATC TCGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC 
TGTGACAACT GCCGCAGACC TGGGGGTGAA CCCAGTCCCG AAGGCACTAC TGGCCAGTCC 
TACAACCAGT ATTCTCAGAG ATACCATCAG AGAACAAACA CTAATGTTAA TTGCCCAATT 
GAGTGCTTCA TGCCTTTAGA TGTACAGGCT GACAGAGAAG ATTCCCGAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( XM_017003693.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859182.1 Homo sapiens fibronectin 1 (FN1), transcript variant X14, mRNA.

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CloneID	OHu89216
Clone ID Related Accession (Same CDS sequence)	XM_017003693.1 , NM_001365521.1
Accession Version	XM_017003693.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	fibronectin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT 
CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT 
CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA 
CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA 
GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT 
CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT 
ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT 
CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT 
GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT 
GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA 
CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA 
AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA 
ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC 
GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG 
GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA 
TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG 
ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG 
GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG 
ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA 
ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC 
AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC 
TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG 
CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG 
CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT 
ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT 
CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGACG AGCTTCCCCA ACTGGTAACC 
CTTCCACACC CCAATCTTCA TGGACCAGAG ATCTTGGATG TTCCTTCCAC AGTTCAAAAG 
ACCCCTTTCG TCACCCACCC TGGGTATGAC ACTGGAAATG GTATTCAGCT TCCTGGCACT 
TCTGGTCAGC AACCCAGTGT TGGGCAACAA ATGATCTTTG AGGAACATGG TTTTAGGCGG 
ACCACACCGC CCACAACGGC CACCCCCATA AGGCATAGGC CAAGACCATA CCCGCCGAAT 
GTAGGACAAG AAGCTCTCTC TCAGACAACC ATCTCATGGG CCCCATTCCA GGACACTTCT 
GAGTACATCA TTTCATGTCA TCCTGTTGGC ACTGATGAAG AACCCTTACA GTTCAGGGTT 
CCTGGAACTT CTACCAGTGC CACTCTGACA GGCCTCACCA GAGGTGCCAC CTACAACGTC 
ATAGTGGAGG CACTGAAAGA CCAGCAGAGG CATAAGGTTC GGGAAGAGGT TGTTACCGTG 
GGCAACTCTG TCAACGAAGG CTTGAACCAA CCTACGGATG ACTCGTGCTT TGACCCCTAC 
ACAGTTTCCC ATTATGCCGT TGGAGATGAG TGGGAACGAA TGTCTGAATC AGGCTTTAAA 
CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT GGTCATTTCA GATGTGATTC ATCTAGATGG 
TGCCATGACA ATGGTGTGAA CTACAAGATT GGAGAGAAGT GGGACCGTCA GGGAGAAAAT 
GGCCAGATGA TGAGCTGCAC ATGTCTTGGG AACGGAAAAG GAGAATTCAA GTGTGACCCT 
CATGAGGCAA CGTGTTATGA TGATGGGAAG ACATACCACG TAGGAGAACA GTGGCAGAAG 
GAATATCTCG GTGCCATTTG CTCCTGCACA TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT 
GACAACTGCC GCAGACCTGG GGGTGAACCC AGTCCCGAAG GCACTACTGG CCAGTCCTAC 
AACCAGTATT CTCAGAGATA CCATCAGAGA ACAAACACTA ATGTTAATTG CCCAATTGAG 
TGCTTCATGC CTTTAGATGT ACAGGCTGAC AGAGAAGATT CCCGAGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859182.1
CDS	366..7436
Translation	MLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSGESQPLVQTAVTTIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPSTVQKTPFVTHPGYDTGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPIRHRPRPYPPNVGQEALSQTTISWAPFQDTSEYIISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGATYNVIVEALKDQQRHKVREEVVTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWERMSESGFKLLCQCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVNCPIECFMPLDVQADREDSRE

Target ORF information:

RefSeq Version	XM_017003693.1
Organism	Homo sapiens(human)
Definition	Homo sapiens fibronectin 1 (FN1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003693.1

ORF Insert Sequence:

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT 
CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT 
CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA 
CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA 
GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT 
CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT 
ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT 
CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT 
GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT 
GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA 
CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA 
AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA 
ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC 
GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG 
GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA 
TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG 
ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG 
GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG 
ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA 
ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC 
AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC 
TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG 
CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG 
CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT 
ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT 
CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGACG AGCTTCCCCA ACTGGTAACC 
CTTCCACACC CCAATCTTCA TGGACCAGAG ATCTTGGATG TTCCTTCCAC AGTTCAAAAG 
ACCCCTTTCG TCACCCACCC TGGGTATGAC ACTGGAAATG GTATTCAGCT TCCTGGCACT 
TCTGGTCAGC AACCCAGTGT TGGGCAACAA ATGATCTTTG AGGAACATGG TTTTAGGCGG 
ACCACACCGC CCACAACGGC CACCCCCATA AGGCATAGGC CAAGACCATA CCCGCCGAAT 
GTAGGACAAG AAGCTCTCTC TCAGACAACC ATCTCATGGG CCCCATTCCA GGACACTTCT 
GAGTACATCA TTTCATGTCA TCCTGTTGGC ACTGATGAAG AACCCTTACA GTTCAGGGTT 
CCTGGAACTT CTACCAGTGC CACTCTGACA GGCCTCACCA GAGGTGCCAC CTACAACGTC 
ATAGTGGAGG CACTGAAAGA CCAGCAGAGG CATAAGGTTC GGGAAGAGGT TGTTACCGTG 
GGCAACTCTG TCAACGAAGG CTTGAACCAA CCTACGGATG ACTCGTGCTT TGACCCCTAC 
ACAGTTTCCC ATTATGCCGT TGGAGATGAG TGGGAACGAA TGTCTGAATC AGGCTTTAAA 
CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT GGTCATTTCA GATGTGATTC ATCTAGATGG 
TGCCATGACA ATGGTGTGAA CTACAAGATT GGAGAGAAGT GGGACCGTCA GGGAGAAAAT 
GGCCAGATGA TGAGCTGCAC ATGTCTTGGG AACGGAAAAG GAGAATTCAA GTGTGACCCT 
CATGAGGCAA CGTGTTATGA TGATGGGAAG ACATACCACG TAGGAGAACA GTGGCAGAAG 
GAATATCTCG GTGCCATTTG CTCCTGCACA TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT 
GACAACTGCC GCAGACCTGG GGGTGAACCC AGTCCCGAAG GCACTACTGG CCAGTCCTAC 
AACCAGTATT CTCAGAGATA CCATCAGAGA ACAAACACTA ATGTTAATTG CCCAATTGAG 
TGCTTCATGC CTTTAGATGT ACAGGCTGAC AGAGAAGATT CCCGAGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( XM_017003694.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859183.1 Homo sapiens fibronectin 1 (FN1), transcript variant X16, mRNA.

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CloneID	OHu89217
Clone ID Related Accession (Same CDS sequence)	NM_001365522.1 , XM_017003694.1
Accession Version	XM_017003694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	fibronectin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT 
CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT 
CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA 
CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA 
GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT 
CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT 
ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT 
CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT 
GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT 
GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA 
CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA 
AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA 
ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC 
GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG 
GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA 
TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG 
ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG 
GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG 
ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA 
ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC 
AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC 
TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG 
CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG 
CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT 
ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT 
CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGTTC AAAAGACCCC TTTCGTCACC 
CACCCTGGGT ATGACACTGG AAATGGTATT CAGCTTCCTG GCACTTCTGG TCAGCAACCC 
AGTGTTGGGC AACAAATGAT CTTTGAGGAA CATGGTTTTA GGCGGACCAC ACCGCCCACA 
ACGGCCACCC CCATAAGGCA TAGGCCAAGA CCATACCCGC CGAATGTAGG ACAAGAAGCT 
CTCTCTCAGA CAACCATCTC ATGGGCCCCA TTCCAGGACA CTTCTGAGTA CATCATTTCA 
TGTCATCCTG TTGGCACTGA TGAAGAACCC TTACAGTTCA GGGTTCCTGG AACTTCTACC 
AGTGCCACTC TGACAGGCCT CACCAGAGGT GCCACCTACA ACGTCATAGT GGAGGCACTG 
AAAGACCAGC AGAGGCATAA GGTTCGGGAA GAGGTTGTTA CCGTGGGCAA CTCTGTCAAC 
GAAGGCTTGA ACCAACCTAC GGATGACTCG TGCTTTGACC CCTACACAGT TTCCCATTAT 
GCCGTTGGAG ATGAGTGGGA ACGAATGTCT GAATCAGGCT TTAAACTGTT GTGCCAGTGC 
TTAGGCTTTG GAAGTGGTCA TTTCAGATGT GATTCATCTA GATGGTGCCA TGACAATGGT 
GTGAACTACA AGATTGGAGA GAAGTGGGAC CGTCAGGGAG AAAATGGCCA GATGATGAGC 
TGCACATGTC TTGGGAACGG AAAAGGAGAA TTCAAGTGTG ACCCTCATGA GGCAACGTGT 
TATGATGATG GGAAGACATA CCACGTAGGA GAACAGTGGC AGAAGGAATA TCTCGGTGCC 
ATTTGCTCCT GCACATGCTT TGGAGGCCAG CGGGGCTGGC GCTGTGACAA CTGCCGCAGA 
CCTGGGGGTG AACCCAGTCC CGAAGGCACT ACTGGCCAGT CCTACAACCA GTATTCTCAG 
AGATACCATC AGAGAACAAA CACTAATGTT AATTGCCCAA TTGAGTGCTT CATGCCTTTA 
GATGTACAGG CTGACAGAGA AGATTCCCGA GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859183.1
CDS	366..7361
Translation	MLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSGESQPLVQTAVTTIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTVQKTPFVTHPGYDTGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPIRHRPRPYPPNVGQEALSQTTISWAPFQDTSEYIISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGATYNVIVEALKDQQRHKVREEVVTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWERMSESGFKLLCQCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVNCPIECFMPLDVQADREDSRE

Target ORF information:

RefSeq Version	XM_017003694.1
Organism	Homo sapiens(human)
Definition	Homo sapiens fibronectin 1 (FN1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003694.1

ORF Insert Sequence:

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT 
CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT 
CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA 
CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA 
GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT 
CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT 
ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT 
CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT 
GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT 
GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA 
CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA 
AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA 
ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC 
GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG 
GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA 
TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG 
ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG 
GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG 
ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA 
ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC 
AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC 
TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG 
CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG 
CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT 
ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT 
CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGTTC AAAAGACCCC TTTCGTCACC 
CACCCTGGGT ATGACACTGG AAATGGTATT CAGCTTCCTG GCACTTCTGG TCAGCAACCC 
AGTGTTGGGC AACAAATGAT CTTTGAGGAA CATGGTTTTA GGCGGACCAC ACCGCCCACA 
ACGGCCACCC CCATAAGGCA TAGGCCAAGA CCATACCCGC CGAATGTAGG ACAAGAAGCT 
CTCTCTCAGA CAACCATCTC ATGGGCCCCA TTCCAGGACA CTTCTGAGTA CATCATTTCA 
TGTCATCCTG TTGGCACTGA TGAAGAACCC TTACAGTTCA GGGTTCCTGG AACTTCTACC 
AGTGCCACTC TGACAGGCCT CACCAGAGGT GCCACCTACA ACGTCATAGT GGAGGCACTG 
AAAGACCAGC AGAGGCATAA GGTTCGGGAA GAGGTTGTTA CCGTGGGCAA CTCTGTCAAC 
GAAGGCTTGA ACCAACCTAC GGATGACTCG TGCTTTGACC CCTACACAGT TTCCCATTAT 
GCCGTTGGAG ATGAGTGGGA ACGAATGTCT GAATCAGGCT TTAAACTGTT GTGCCAGTGC 
TTAGGCTTTG GAAGTGGTCA TTTCAGATGT GATTCATCTA GATGGTGCCA TGACAATGGT 
GTGAACTACA AGATTGGAGA GAAGTGGGAC CGTCAGGGAG AAAATGGCCA GATGATGAGC 
TGCACATGTC TTGGGAACGG AAAAGGAGAA TTCAAGTGTG ACCCTCATGA GGCAACGTGT 
TATGATGATG GGAAGACATA CCACGTAGGA GAACAGTGGC AGAAGGAATA TCTCGGTGCC 
ATTTGCTCCT GCACATGCTT TGGAGGCCAG CGGGGCTGGC GCTGTGACAA CTGCCGCAGA 
CCTGGGGGTG AACCCAGTCC CGAAGGCACT ACTGGCCAGT CCTACAACCA GTATTCTCAG 
AGATACCATC AGAGAACAAA CACTAATGTT AATTGCCCAA TTGAGTGCTT CATGCCTTTA 
GATGTACAGG CTGACAGAGA AGATTCCCGA GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( XM_017003695.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859184.1 Homo sapiens fibronectin 1 (FN1), transcript variant X20, mRNA.

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CloneID	OHu89218
Clone ID Related Accession (Same CDS sequence)	XM_017003695.1
Accession Version	XM_017003695.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	fibronectin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA 
GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG 
CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC 
AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT 
GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC 
CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT 
CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA 
CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA 
GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG 
ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC 
CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC 
ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG 
TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT 
GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA 
GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC 
AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT 
GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA 
GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG 
AGTCAGCCTC TGGTTCAGAC TGCAGTAACC ACTATTCCTG CACCAACTGA CCTGAAGTTC 
ACTCAGGTCA CACCCACAAG CCTGAGCGCC CAGTGGACAC CACCCAATGT TCAGCTCACT 
GGATATCGAG TGCGGGTGAC CCCCAAGGAG AAGACCGGAC CAATGAAAGA AATCAACCTT 
GCTCCTGACA GCTCATCCGT GGTTGTATCA GGACTTATGG TGGCCACCAA ATATGAAGTG 
AGTGTCTATG CTCTTAAGGA CACTTTGACA AGCAGACCAG CTCAGGGAGT TGTCACCACT 
CTGGAGAATG TCAGCCCACC AAGAAGGGCT CGTGTGACAG ATGCTACTGA GACCACCATC 
ACCATTAGCT GGAGAACCAA GACTGAGACG ATCACTGGCT TCCAAGTTGA TGCCGTTCCA 
GCCAATGGCC AGACTCCAAT CCAGAGAACC ATCAAGCCAG ATGTCAGAAG CTACACCATC 
ACAGGTTTAC AACCAGGCAC TGACTACAAG ATCTACCTGT ACACCTTGAA TGACAATGCT 
CGGAGCTCCC CTGTGGTCAT CGACGCCTCC ACTGCCATTG ATGCACCATC CAACCTGCGT 
TTCCTGGCCA CCACACCCAA TTCCTTGCTG GTATCATGGC AGCCGCCACG TGCCAGGATT 
ACCGGCTACA TCATCAAGTA TGAGAAGCCT GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG 
CCCCGCCCTG GTGTCACAGA GGCTACTATT ACTGGCCTGG AACCGGGAAC CGAATATACA 
ATTTATGTCA TTGCCCTGAA GAATAATCAG AAGAGCGAGC CCCTGATTGG AAGGAAAAAG 
ACAGACGAGC TTCCCCAACT GGTAACCCTT CCACACCCCA ATCTTCATGG ACCAGAGATC 
TTGGATGTTC CTTCCACAGT TCAAAAGACC CCTTTCGTCA CCCACCCTGG GTATGACACT 
GGAAATGGTA TTCAGCTTCC TGGCACTTCT GGTCAGCAAC CCAGTGTTGG GCAACAAATG 
ATCTTTGAGG AACATGGTTT TAGGCGGACC ACACCGCCCA CAACGGCCAC CCCCATAAGG 
CATAGGCCAA GACCATACCC GCCGAATGTA GGTGAGGAAA TCCAAATTGG TCACATCCCC 
AGGGAAGATG TAGACTATCA CCTGTACCCA CACGGTCCGG GACTCAATCC AAATGCCTCT 
ACAGGACAAG AAGCTCTCTC TCAGACAACC ATCTCATGGG CCCCATTCCA GGACACTTCT 
GAGTACATCA TTTCATGTCA TCCTGTTGGC ACTGATGAAG AACCCTTACA GTTCAGGGTT 
CCTGGAACTT CTACCAGTGC CACTCTGACA GGCCTCACCA GAGGTGCCAC CTACAACGTC 
ATAGTGGAGG CACTGAAAGA CCAGCAGAGG CATAAGGTTC GGGAAGAGGT TGTTACCGTG 
GGCAACTCTG TCAACGAAGG CTTGAACCAA CCTACGGATG ACTCGTGCTT TGACCCCTAC 
ACAGTTTCCC ATTATGCCGT TGGAGATGAG TGGGAACGAA TGTCTGAATC AGGCTTTAAA 
CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT GGTCATTTCA GATGTGATTC ATCTAGATGG 
TGCCATGACA ATGGTGTGAA CTACAAGATT GGAGAGAAGT GGGACCGTCA GGGAGAAAAT 
GGCCAGATGA TGAGCTGCAC ATGTCTTGGG AACGGAAAAG GAGAATTCAA GTGTGACCCT 
CATGAGGCAA CGTGTTATGA TGATGGGAAG ACATACCACG TAGGAGAACA GTGGCAGAAG 
GAATATCTCG GTGCCATTTG CTCCTGCACA TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT 
GACAACTGCC GCAGACCTGG GGGTGAACCC AGTCCCGAAG GCACTACTGG CCAGTCCTAC 
AACCAGTATT CTCAGAGATA CCATCAGAGA ACAAACACTG TAAGTGCATT AGCAGCACAA 
GTGTGTTCCC TCATACTAGA CAGTCTCTTT CTACAGGTAT CTTTCTTCAG AATGAACCAA 
GTGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859184.1
CDS	366..7274
Translation	MLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIPAVPPPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSGESQPLVQTAVTTIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPSTVQKTPFVTHPGYDTGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPIRHRPRPYPPNVGEEIQIGHIPREDVDYHLYPHGPGLNPNASTGQEALSQTTISWAPFQDTSEYIISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGATYNVIVEALKDQQRHKVREEVVTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWERMSESGFKLLCQCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTVSALAAQVCSLILDSLFLQVSFFRMNQVF

Target ORF information:

RefSeq Version	XM_017003695.1
Organism	Homo sapiens(human)
Definition	Homo sapiens fibronectin 1 (FN1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003695.1

ORF Insert Sequence:

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA 
GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG 
CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC 
AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT 
GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC 
CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT 
CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA 
CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA 
GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG 
ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC 
CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC 
ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG 
TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT 
GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA 
GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC 
AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT 
GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA 
GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG 
AGTCAGCCTC TGGTTCAGAC TGCAGTAACC ACTATTCCTG CACCAACTGA CCTGAAGTTC 
ACTCAGGTCA CACCCACAAG CCTGAGCGCC CAGTGGACAC CACCCAATGT TCAGCTCACT 
GGATATCGAG TGCGGGTGAC CCCCAAGGAG AAGACCGGAC CAATGAAAGA AATCAACCTT 
GCTCCTGACA GCTCATCCGT GGTTGTATCA GGACTTATGG TGGCCACCAA ATATGAAGTG 
AGTGTCTATG CTCTTAAGGA CACTTTGACA AGCAGACCAG CTCAGGGAGT TGTCACCACT 
CTGGAGAATG TCAGCCCACC AAGAAGGGCT CGTGTGACAG ATGCTACTGA GACCACCATC 
ACCATTAGCT GGAGAACCAA GACTGAGACG ATCACTGGCT TCCAAGTTGA TGCCGTTCCA 
GCCAATGGCC AGACTCCAAT CCAGAGAACC ATCAAGCCAG ATGTCAGAAG CTACACCATC 
ACAGGTTTAC AACCAGGCAC TGACTACAAG ATCTACCTGT ACACCTTGAA TGACAATGCT 
CGGAGCTCCC CTGTGGTCAT CGACGCCTCC ACTGCCATTG ATGCACCATC CAACCTGCGT 
TTCCTGGCCA CCACACCCAA TTCCTTGCTG GTATCATGGC AGCCGCCACG TGCCAGGATT 
ACCGGCTACA TCATCAAGTA TGAGAAGCCT GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG 
CCCCGCCCTG GTGTCACAGA GGCTACTATT ACTGGCCTGG AACCGGGAAC CGAATATACA 
ATTTATGTCA TTGCCCTGAA GAATAATCAG AAGAGCGAGC CCCTGATTGG AAGGAAAAAG 
ACAGACGAGC TTCCCCAACT GGTAACCCTT CCACACCCCA ATCTTCATGG ACCAGAGATC 
TTGGATGTTC CTTCCACAGT TCAAAAGACC CCTTTCGTCA CCCACCCTGG GTATGACACT 
GGAAATGGTA TTCAGCTTCC TGGCACTTCT GGTCAGCAAC CCAGTGTTGG GCAACAAATG 
ATCTTTGAGG AACATGGTTT TAGGCGGACC ACACCGCCCA CAACGGCCAC CCCCATAAGG 
CATAGGCCAA GACCATACCC GCCGAATGTA GGTGAGGAAA TCCAAATTGG TCACATCCCC 
AGGGAAGATG TAGACTATCA CCTGTACCCA CACGGTCCGG GACTCAATCC AAATGCCTCT 
ACAGGACAAG AAGCTCTCTC TCAGACAACC ATCTCATGGG CCCCATTCCA GGACACTTCT 
GAGTACATCA TTTCATGTCA TCCTGTTGGC ACTGATGAAG AACCCTTACA GTTCAGGGTT 
CCTGGAACTT CTACCAGTGC CACTCTGACA GGCCTCACCA GAGGTGCCAC CTACAACGTC 
ATAGTGGAGG CACTGAAAGA CCAGCAGAGG CATAAGGTTC GGGAAGAGGT TGTTACCGTG 
GGCAACTCTG TCAACGAAGG CTTGAACCAA CCTACGGATG ACTCGTGCTT TGACCCCTAC 
ACAGTTTCCC ATTATGCCGT TGGAGATGAG TGGGAACGAA TGTCTGAATC AGGCTTTAAA 
CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT GGTCATTTCA GATGTGATTC ATCTAGATGG 
TGCCATGACA ATGGTGTGAA CTACAAGATT GGAGAGAAGT GGGACCGTCA GGGAGAAAAT 
GGCCAGATGA TGAGCTGCAC ATGTCTTGGG AACGGAAAAG GAGAATTCAA GTGTGACCCT 
CATGAGGCAA CGTGTTATGA TGATGGGAAG ACATACCACG TAGGAGAACA GTGGCAGAAG 
GAATATCTCG GTGCCATTTG CTCCTGCACA TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT 
GACAACTGCC GCAGACCTGG GGGTGAACCC AGTCCCGAAG GCACTACTGG CCAGTCCTAC 
AACCAGTATT CTCAGAGATA CCATCAGAGA ACAAACACTG TAAGTGCATT AGCAGCACAA 
GTGTGTTCCC TCATACTAGA CAGTCTCTTT CTACAGGTAT CTTTCTTCAG AATGAACCAA 
GTGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( NM_212476.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_997641.1 Homo sapiens fibronectin 1 (FN1), transcript variant 5, mRNA.

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CloneID	OHu24915
Clone ID Related Accession (Same CDS sequence)	NM_212476.2
Accession Version	NM_212476.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	fibronectin isoform 5 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX640608.1, AJ535086.1, X02761.1, AI033037.1, U60068.1, BQ005645.1 and CR749317.1. On Jan 9, 2016 this sequence version replaced NM_212476.1. Transcript Variant: This variant (5) lacks two in-frame exons in the coding region, as compared to variant 1. Isoform 5 encoded by this variant thus lacks two internal segments, as compared to isoform 1. The encoded isoform (5) may undergo proteolytic processing similar to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX640608.1, DRR138524.403056.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA 
GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG 
CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC 
AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT 
GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC 
CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT 
CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA 
CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA 
GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG 
ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC 
CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC 
ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG 
TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT 
GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA 
GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC 
AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT 
GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA 
GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG 
AGTCAGCCTC TGGTTCAGAC TGCAGTAACC ACTATTCCTG CACCAACTGA CCTGAAGTTC 
ACTCAGGTCA CACCCACAAG CCTGAGCGCC CAGTGGACAC CACCCAATGT TCAGCTCACT 
GGATATCGAG TGCGGGTGAC CCCCAAGGAG AAGACCGGAC CAATGAAAGA AATCAACCTT 
GCTCCTGACA GCTCATCCGT GGTTGTATCA GGACTTATGG TGGCCACCAA ATATGAAGTG 
AGTGTCTATG CTCTTAAGGA CACTTTGACA AGCAGACCAG CTCAGGGAGT TGTCACCACT 
CTGGAGAATG TCAGCCCACC AAGAAGGGCT CGTGTGACAG ATGCTACTGA GACCACCATC 
ACCATTAGCT GGAGAACCAA GACTGAGACG ATCACTGGCT TCCAAGTTGA TGCCGTTCCA 
GCCAATGGCC AGACTCCAAT CCAGAGAACC ATCAAGCCAG ATGTCAGAAG CTACACCATC 
ACAGGTTTAC AACCAGGCAC TGACTACAAG ATCTACCTGT ACACCTTGAA TGACAATGCT 
CGGAGCTCCC CTGTGGTCAT CGACGCCTCC ACTGCCATTG ATGCACCATC CAACCTGCGT 
TTCCTGGCCA CCACACCCAA TTCCTTGCTG GTATCATGGC AGCCGCCACG TGCCAGGATT 
ACCGGCTACA TCATCAAGTA TGAGAAGCCT GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG 
CCCCGCCCTG GTGTCACAGA GGCTACTATT ACTGGCCTGG AACCGGGAAC CGAATATACA 
ATTTATGTCA TTGCCCTGAA GAATAATCAG AAGAGCGAGC CCCTGATTGG AAGGAAAAAG 
ACAGACGAGC TTCCCCAACT GGTAACCCTT CCACACCCCA ATCTTCATGG ACCAGAGATC 
TTGGATGTTC CTTCCACAGT TCAAAAGACC CCTTTCGTCA CCCACCCTGG GTATGACACT 
GGAAATGGTA TTCAGCTTCC TGGCACTTCT GGTCAGCAAC CCAGTGTTGG GCAACAAATG 
ATCTTTGAGG AACATGGTTT TAGGCGGACC ACACCGCCCA CAACGGCCAC CCCCATAAGG 
CATAGGCCAA GACCATACCC GCCGAATGTA GGTGAGGAAA TCCAAATTGG TCACATCCCC 
AGGGAAGATG TAGACTATCA CCTGTACCCA CACGGTCCGG GACTCAATCC AAATGCCTCT 
ACAGGACAAG AAGCTCTCTC TCAGACAACC ATCTCATGGG CCCCATTCCA GGACACTTCT 
GAGTACATCA TTTCATGTCA TCCTGTTGGC ACTGATGAAG AACCCTTACA GTTCAGGGTT 
CCTGGAACTT CTACCAGTGC CACTCTGACA GGCCTCACCA GAGGTGCCAC CTACAACATC 
ATAGTGGAGG CACTGAAAGA CCAGCAGAGG CATAAGGTTC GGGAAGAGGT TGTTACCGTG 
GGCAACTCTG TCAACGAAGG CTTGAACCAA CCTACGGATG ACTCGTGCTT TGACCCCTAC 
ACAGTTTCCC ATTATGCCGT TGGAGATGAG TGGGAACGAA TGTCTGAATC AGGCTTTAAA 
CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT GGTCATTTCA GATGTGATTC ATCTAGATGG 
TGCCATGACA ATGGTGTGAA CTACAAGATT GGAGAGAAGT GGGACCGTCA GGGAGAAAAT 
GGCCAGATGA TGAGCTGCAC ATGTCTTGGG AACGGAAAAG GAGAATTCAA GTGTGACCCT 
CATGAGGCAA CGTGTTATGA TGATGGGAAG ACATACCACG TAGGAGAACA GTGGCAGAAG 
GAATATCTCG GTGCCATTTG CTCCTGCACA TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT 
GACAACTGCC GCAGACCTGG GGGTGAACCC AGTCCCGAAG GCACTACTGG CCAGTCCTAC 
AACCAGTATT CTCAGAGATA CCATCAGAGA ACAAACACTA ATGTTAATTG CCCAATTGAG 
TGCTTCATGC CTTTAGATGT ACAGGCTGAC AGAGAAGATT CCCGAGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997641.1
CDS	267..7157
Translation	MLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDPTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIPAVPPPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSGESQPLVQTAVTTIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPSTVQKTPFVTHPGYDTGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPIRHRPRPYPPNVGEEIQIGHIPREDVDYHLYPHGPGLNPNASTGQEALSQTTISWAPFQDTSEYIISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGATYNIIVEALKDQQRHKVREEVVTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWERMSESGFKLLCQCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVNCPIECFMPLDVQADREDSRE

Target ORF information:

RefSeq Version	NM_212476.2
Organism	Homo sapiens(human)
Definition	Homo sapiens fibronectin 1 (FN1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212476.2

ORF Insert Sequence:

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA 
GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG 
CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC 
AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT 
GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC 
CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT 
CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA 
CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA 
GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG 
ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC 
CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC 
ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG 
TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT 
GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA 
GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC 
AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT 
GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA 
GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG 
AGTCAGCCTC TGGTTCAGAC TGCAGTAACC ACTATTCCTG CACCAACTGA CCTGAAGTTC 
ACTCAGGTCA CACCCACAAG CCTGAGCGCC CAGTGGACAC CACCCAATGT TCAGCTCACT 
GGATATCGAG TGCGGGTGAC CCCCAAGGAG AAGACCGGAC CAATGAAAGA AATCAACCTT 
GCTCCTGACA GCTCATCCGT GGTTGTATCA GGACTTATGG TGGCCACCAA ATATGAAGTG 
AGTGTCTATG CTCTTAAGGA CACTTTGACA AGCAGACCAG CTCAGGGAGT TGTCACCACT 
CTGGAGAATG TCAGCCCACC AAGAAGGGCT CGTGTGACAG ATGCTACTGA GACCACCATC 
ACCATTAGCT GGAGAACCAA GACTGAGACG ATCACTGGCT TCCAAGTTGA TGCCGTTCCA 
GCCAATGGCC AGACTCCAAT CCAGAGAACC ATCAAGCCAG ATGTCAGAAG CTACACCATC 
ACAGGTTTAC AACCAGGCAC TGACTACAAG ATCTACCTGT ACACCTTGAA TGACAATGCT 
CGGAGCTCCC CTGTGGTCAT CGACGCCTCC ACTGCCATTG ATGCACCATC CAACCTGCGT 
TTCCTGGCCA CCACACCCAA TTCCTTGCTG GTATCATGGC AGCCGCCACG TGCCAGGATT 
ACCGGCTACA TCATCAAGTA TGAGAAGCCT GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG 
CCCCGCCCTG GTGTCACAGA GGCTACTATT ACTGGCCTGG AACCGGGAAC CGAATATACA 
ATTTATGTCA TTGCCCTGAA GAATAATCAG AAGAGCGAGC CCCTGATTGG AAGGAAAAAG 
ACAGACGAGC TTCCCCAACT GGTAACCCTT CCACACCCCA ATCTTCATGG ACCAGAGATC 
TTGGATGTTC CTTCCACAGT TCAAAAGACC CCTTTCGTCA CCCACCCTGG GTATGACACT 
GGAAATGGTA TTCAGCTTCC TGGCACTTCT GGTCAGCAAC CCAGTGTTGG GCAACAAATG 
ATCTTTGAGG AACATGGTTT TAGGCGGACC ACACCGCCCA CAACGGCCAC CCCCATAAGG 
CATAGGCCAA GACCATACCC GCCGAATGTA GGTGAGGAAA TCCAAATTGG TCACATCCCC 
AGGGAAGATG TAGACTATCA CCTGTACCCA CACGGTCCGG GACTCAATCC AAATGCCTCT 
ACAGGACAAG AAGCTCTCTC TCAGACAACC ATCTCATGGG CCCCATTCCA GGACACTTCT 
GAGTACATCA TTTCATGTCA TCCTGTTGGC ACTGATGAAG AACCCTTACA GTTCAGGGTT 
CCTGGAACTT CTACCAGTGC CACTCTGACA GGCCTCACCA GAGGTGCCAC CTACAACATC 
ATAGTGGAGG CACTGAAAGA CCAGCAGAGG CATAAGGTTC GGGAAGAGGT TGTTACCGTG 
GGCAACTCTG TCAACGAAGG CTTGAACCAA CCTACGGATG ACTCGTGCTT TGACCCCTAC 
ACAGTTTCCC ATTATGCCGT TGGAGATGAG TGGGAACGAA TGTCTGAATC AGGCTTTAAA 
CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT GGTCATTTCA GATGTGATTC ATCTAGATGG 
TGCCATGACA ATGGTGTGAA CTACAAGATT GGAGAGAAGT GGGACCGTCA GGGAGAAAAT 
GGCCAGATGA TGAGCTGCAC ATGTCTTGGG AACGGAAAAG GAGAATTCAA GTGTGACCCT 
CATGAGGCAA CGTGTTATGA TGATGGGAAG ACATACCACG TAGGAGAACA GTGGCAGAAG 
GAATATCTCG GTGCCATTTG CTCCTGCACA TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT 
GACAACTGCC GCAGACCTGG GGGTGAACCC AGTCCCGAAG GCACTACTGG CCAGTCCTAC 
AACCAGTATT CTCAGAGATA CCATCAGAGA ACAAACACTA ATGTTAATTG CCCAATTGAG 
TGCTTCATGC CTTTAGATGT ACAGGCTGAC AGAGAAGATT CCCGAGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( NM_212474.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_997639.1 Homo sapiens fibronectin 1 (FN1), transcript variant 6, mRNA.

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CloneID	OHu24997
Clone ID Related Accession (Same CDS sequence)	NM_212474.2
Accession Version	NM_212474.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	fibronectin isoform 6 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL832202.1, AJ535086.1, X02761.1, AI033037.1, U60068.1, BQ005645.1 and CR749317.1. On Jan 9, 2016 this sequence version replaced NM_212474.1. Transcript Variant: This variant (6) lacks two in-frame exons and an in-frame segment in the coding region, as compared to variant 1. Isoform 6 encoded by this variant thus lacks three internal segments, as compared to isoform 1. The encoded isoform (6) may undergo proteolytic processing similar to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC078656.1, SRR1660803.174926.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA 
GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG 
CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC 
AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT 
GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC 
CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT 
CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA 
CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA 
GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG 
ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC 
CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC 
ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG 
TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT 
GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA 
GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC 
AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT 
GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA 
GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG 
AGTCAGCCTC TGGTTCAGAC TGCAGTAACC ACTATTCCTG CACCAACTGA CCTGAAGTTC 
ACTCAGGTCA CACCCACAAG CCTGAGCGCC CAGTGGACAC CACCCAATGT TCAGCTCACT 
GGATATCGAG TGCGGGTGAC CCCCAAGGAG AAGACCGGAC CAATGAAAGA AATCAACCTT 
GCTCCTGACA GCTCATCCGT GGTTGTATCA GGACTTATGG TGGCCACCAA ATATGAAGTG 
AGTGTCTATG CTCTTAAGGA CACTTTGACA AGCAGACCAG CTCAGGGAGT TGTCACCACT 
CTGGAGAATG TCAGCCCACC AAGAAGGGCT CGTGTGACAG ATGCTACTGA GACCACCATC 
ACCATTAGCT GGAGAACCAA GACTGAGACG ATCACTGGCT TCCAAGTTGA TGCCGTTCCA 
GCCAATGGCC AGACTCCAAT CCAGAGAACC ATCAAGCCAG ATGTCAGAAG CTACACCATC 
ACAGGTTTAC AACCAGGCAC TGACTACAAG ATCTACCTGT ACACCTTGAA TGACAATGCT 
CGGAGCTCCC CTGTGGTCAT CGACGCCTCC ACTGCCATTG ATGCACCATC CAACCTGCGT 
TTCCTGGCCA CCACACCCAA TTCCTTGCTG GTATCATGGC AGCCGCCACG TGCCAGGATT 
ACCGGCTACA TCATCAAGTA TGAGAAGCCT GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG 
CCCCGCCCTG GTGTCACAGA GGCTACTATT ACTGGCCTGG AACCGGGAAC CGAATATACA 
ATTTATGTCA TTGCCCTGAA GAATAATCAG AAGAGCGAGC CCCTGATTGG AAGGAAAAAG 
ACAGGACAAG AAGCTCTCTC TCAGACAACC ATCTCATGGG CCCCATTCCA GGACACTTCT 
GAGTACATCA TTTCATGTCA TCCTGTTGGC ACTGATGAAG AACCCTTACA GTTCAGGGTT 
CCTGGAACTT CTACCAGTGC CACTCTGACA GGCCTCACCA GAGGTGCCAC CTACAACATC 
ATAGTGGAGG CACTGAAAGA CCAGCAGAGG CATAAGGTTC GGGAAGAGGT TGTTACCGTG 
GGCAACTCTG TCAACGAAGG CTTGAACCAA CCTACGGATG ACTCGTGCTT TGACCCCTAC 
ACAGTTTCCC ATTATGCCGT TGGAGATGAG TGGGAACGAA TGTCTGAATC AGGCTTTAAA 
CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT GGTCATTTCA GATGTGATTC ATCTAGATGG 
TGCCATGACA ATGGTGTGAA CTACAAGATT GGAGAGAAGT GGGACCGTCA GGGAGAAAAT 
GGCCAGATGA TGAGCTGCAC ATGTCTTGGG AACGGAAAAG GAGAATTCAA GTGTGACCCT 
CATGAGGCAA CGTGTTATGA TGATGGGAAG ACATACCACG TAGGAGAACA GTGGCAGAAG 
GAATATCTCG GTGCCATTTG CTCCTGCACA TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT 
GACAACTGCC GCAGACCTGG GGGTGAACCC AGTCCCGAAG GCACTACTGG CCAGTCCTAC 
AACCAGTATT CTCAGAGATA CCATCAGAGA ACAAACACTA ATGTTAATTG CCCAATTGAG 
TGCTTCATGC CTTTAGATGT ACAGGCTGAC AGAGAAGATT CCCGAGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997639.1
CDS	267..6797
Translation	MLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDPTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIPAVPPPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSGESQPLVQTAVTTIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTGQEALSQTTISWAPFQDTSEYIISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGATYNIIVEALKDQQRHKVREEVVTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWERMSESGFKLLCQCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVNCPIECFMPLDVQADREDSRE

Target ORF information:

RefSeq Version	NM_212474.2
Organism	Homo sapiens(human)
Definition	Homo sapiens fibronectin 1 (FN1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212474.2

ORF Insert Sequence:

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA 
GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG 
CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC 
AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT 
GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC 
CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT 
CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA 
CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA 
GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG 
ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC 
CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC 
ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG 
TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT 
GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA 
GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC 
AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT 
GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA 
GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG 
AGTCAGCCTC TGGTTCAGAC TGCAGTAACC ACTATTCCTG CACCAACTGA CCTGAAGTTC 
ACTCAGGTCA CACCCACAAG CCTGAGCGCC CAGTGGACAC CACCCAATGT TCAGCTCACT 
GGATATCGAG TGCGGGTGAC CCCCAAGGAG AAGACCGGAC CAATGAAAGA AATCAACCTT 
GCTCCTGACA GCTCATCCGT GGTTGTATCA GGACTTATGG TGGCCACCAA ATATGAAGTG 
AGTGTCTATG CTCTTAAGGA CACTTTGACA AGCAGACCAG CTCAGGGAGT TGTCACCACT 
CTGGAGAATG TCAGCCCACC AAGAAGGGCT CGTGTGACAG ATGCTACTGA GACCACCATC 
ACCATTAGCT GGAGAACCAA GACTGAGACG ATCACTGGCT TCCAAGTTGA TGCCGTTCCA 
GCCAATGGCC AGACTCCAAT CCAGAGAACC ATCAAGCCAG ATGTCAGAAG CTACACCATC 
ACAGGTTTAC AACCAGGCAC TGACTACAAG ATCTACCTGT ACACCTTGAA TGACAATGCT 
CGGAGCTCCC CTGTGGTCAT CGACGCCTCC ACTGCCATTG ATGCACCATC CAACCTGCGT 
TTCCTGGCCA CCACACCCAA TTCCTTGCTG GTATCATGGC AGCCGCCACG TGCCAGGATT 
ACCGGCTACA TCATCAAGTA TGAGAAGCCT GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG 
CCCCGCCCTG GTGTCACAGA GGCTACTATT ACTGGCCTGG AACCGGGAAC CGAATATACA 
ATTTATGTCA TTGCCCTGAA GAATAATCAG AAGAGCGAGC CCCTGATTGG AAGGAAAAAG 
ACAGGACAAG AAGCTCTCTC TCAGACAACC ATCTCATGGG CCCCATTCCA GGACACTTCT 
GAGTACATCA TTTCATGTCA TCCTGTTGGC ACTGATGAAG AACCCTTACA GTTCAGGGTT 
CCTGGAACTT CTACCAGTGC CACTCTGACA GGCCTCACCA GAGGTGCCAC CTACAACATC 
ATAGTGGAGG CACTGAAAGA CCAGCAGAGG CATAAGGTTC GGGAAGAGGT TGTTACCGTG 
GGCAACTCTG TCAACGAAGG CTTGAACCAA CCTACGGATG ACTCGTGCTT TGACCCCTAC 
ACAGTTTCCC ATTATGCCGT TGGAGATGAG TGGGAACGAA TGTCTGAATC AGGCTTTAAA 
CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT GGTCATTTCA GATGTGATTC ATCTAGATGG 
TGCCATGACA ATGGTGTGAA CTACAAGATT GGAGAGAAGT GGGACCGTCA GGGAGAAAAT 
GGCCAGATGA TGAGCTGCAC ATGTCTTGGG AACGGAAAAG GAGAATTCAA GTGTGACCCT 
CATGAGGCAA CGTGTTATGA TGATGGGAAG ACATACCACG TAGGAGAACA GTGGCAGAAG 
GAATATCTCG GTGCCATTTG CTCCTGCACA TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT 
GACAACTGCC GCAGACCTGG GGGTGAACCC AGTCCCGAAG GCACTACTGG CCAGTCCTAC 
AACCAGTATT CTCAGAGATA CCATCAGAGA ACAAACACTA ATGTTAATTG CCCAATTGAG 
TGCTTCATGC CTTTAGATGT ACAGGCTGAC AGAGAAGATT CCCGAGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( NM_002026.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002017.1 Homo sapiens fibronectin 1 (FN1), transcript variant 3, mRNA.

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CloneID	OHu25005
Clone ID Related Accession (Same CDS sequence)	NM_002026.3
Accession Version	NM_002026.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Homo sapiens(human)
Product	fibronectin isoform 3 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX640731.1, AJ535086.1, BX640802.1, X02761.1, AI033037.1, BX640638.1, BQ005645.1 and CR749317.1. On Jan 9, 2016 this sequence version replaced NM_002026.2. Transcript Variant: This variant (3) lacks an in-frame exon and an internal segment in the coding region, as compared to variant 1. Isoform 3 encoded by this variant thus lacks two internal segments, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX538017.1, BX538018.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA 
GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG 
CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC 
AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT 
GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC 
CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT 
CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA 
CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA 
GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG 
ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC 
CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC 
ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG 
TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT 
GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA 
GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC 
AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT 
GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA 
GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG 
AGTCAGCCTC TGGTTCAGAC TGCAGTAACC AACATTGATC GCCCTAAAGG ACTGGCATTC 
ACTGATGTGG ATGTCGATTC CATCAAAATT GCTTGGGAAA GCCCACAGGG GCAAGTTTCC 
AGGTACAGGG TGACCTACTC GAGCCCTGAG GATGGAATCC ATGAGCTATT CCCTGCACCT 
GATGGTGAAG AAGACACTGC AGAGCTGCAA GGCCTCAGAC CGGGTTCTGA GTACACAGTC 
AGTGTGGTTG CCTTGCACGA TGATATGGAG AGCCAGCCCC TGATTGGAAC CCAGTCCACA 
GCTATTCCTG CACCAACTGA CCTGAAGTTC ACTCAGGTCA CACCCACAAG CCTGAGCGCC 
CAGTGGACAC CACCCAATGT TCAGCTCACT GGATATCGAG TGCGGGTGAC CCCCAAGGAG 
AAGACCGGAC CAATGAAAGA AATCAACCTT GCTCCTGACA GCTCATCCGT GGTTGTATCA 
GGACTTATGG TGGCCACCAA ATATGAAGTG AGTGTCTATG CTCTTAAGGA CACTTTGACA 
AGCAGACCAG CTCAGGGAGT TGTCACCACT CTGGAGAATG TCAGCCCACC AAGAAGGGCT 
CGTGTGACAG ATGCTACTGA GACCACCATC ACCATTAGCT GGAGAACCAA GACTGAGACG 
ATCACTGGCT TCCAAGTTGA TGCCGTTCCA GCCAATGGCC AGACTCCAAT CCAGAGAACC 
ATCAAGCCAG ATGTCAGAAG CTACACCATC ACAGGTTTAC AACCAGGCAC TGACTACAAG 
ATCTACCTGT ACACCTTGAA TGACAATGCT CGGAGCTCCC CTGTGGTCAT CGACGCCTCC 
ACTGCCATTG ATGCACCATC CAACCTGCGT TTCCTGGCCA CCACACCCAA TTCCTTGCTG 
GTATCATGGC AGCCGCCACG TGCCAGGATT ACCGGCTACA TCATCAAGTA TGAGAAGCCT 
GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG CCCCGCCCTG GTGTCACAGA GGCTACTATT 
ACTGGCCTGG AACCGGGAAC CGAATATACA ATTTATGTCA TTGCCCTGAA GAATAATCAG 
AAGAGCGAGC CCCTGATTGG AAGGAAAAAG ACAGACGAGC TTCCCCAACT GGTAACCCTT 
CCACACCCCA ATCTTCATGG ACCAGAGATC TTGGATGTTC CTTCCACAGT TCAAAAGACC 
CCTTTCGTCA CCCACCCTGG GTATGACACT GGAAATGGTA TTCAGCTTCC TGGCACTTCT 
GGTCAGCAAC CCAGTGTTGG GCAACAAATG ATCTTTGAGG AACATGGTTT TAGGCGGACC 
ACACCGCCCA CAACGGCCAC CCCCATAAGG CATAGGCCAA GACCATACCC GCCGAATGTA 
GGACAAGAAG CTCTCTCTCA GACAACCATC TCATGGGCCC CATTCCAGGA CACTTCTGAG 
TACATCATTT CATGTCATCC TGTTGGCACT GATGAAGAAC CCTTACAGTT CAGGGTTCCT 
GGAACTTCTA CCAGTGCCAC TCTGACAGGC CTCACCAGAG GTGCCACCTA CAACATCATA 
GTGGAGGCAC TGAAAGACCA GCAGAGGCAT AAGGTTCGGG AAGAGGTTGT TACCGTGGGC 
AACTCTGTCA ACGAAGGCTT GAACCAACCT ACGGATGACT CGTGCTTTGA CCCCTACACA 
GTTTCCCATT ATGCCGTTGG AGATGAGTGG GAACGAATGT CTGAATCAGG CTTTAAACTG 
TTGTGCCAGT GCTTAGGCTT TGGAAGTGGT CATTTCAGAT GTGATTCATC TAGATGGTGC 
CATGACAATG GTGTGAACTA CAAGATTGGA GAGAAGTGGG ACCGTCAGGG AGAAAATGGC 
CAGATGATGA GCTGCACATG TCTTGGGAAC GGAAAAGGAG AATTCAAGTG TGACCCTCAT 
GAGGCAACGT GTTATGATGA TGGGAAGACA TACCACGTAG GAGAACAGTG GCAGAAGGAA 
TATCTCGGTG CCATTTGCTC CTGCACATGC TTTGGAGGCC AGCGGGGCTG GCGCTGTGAC 
AACTGCCGCA GACCTGGGGG TGAACCCAGT CCCGAAGGCA CTACTGGCCA GTCCTACAAC 
CAGTATTCTC AGAGATACCA TCAGAGAACA AACACTAATG TTAATTGCCC AATTGAGTGC 
TTCATGCCTT TAGATGTACA GGCTGACAGA GAAGATTCCC GAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002017.1
CDS	267..7334
Translation	MLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDPTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIPAVPPPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSGESQPLVQTAVTNIDRPKGLAFTDVDVDSIKIAWESPQGQVSRYRVTYSSPEDGIHELFPAPDGEEDTAELQGLRPGSEYTVSVVALHDDMESQPLIGTQSTAIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPSTVQKTPFVTHPGYDTGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPIRHRPRPYPPNVGQEALSQTTISWAPFQDTSEYIISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGATYNIIVEALKDQQRHKVREEVVTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWERMSESGFKLLCQCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVNCPIECFMPLDVQADREDSRE

Target ORF information:

RefSeq Version	NM_002026.3
Organism	Homo sapiens(human)
Definition	Homo sapiens fibronectin 1 (FN1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002026.3

ORF Insert Sequence:

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA 
GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG 
CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC 
AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT 
GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC 
CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT 
CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA 
CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA 
GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG 
ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC 
CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC 
ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG 
TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT 
GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA 
GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC 
AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT 
GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA 
GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG 
AGTCAGCCTC TGGTTCAGAC TGCAGTAACC AACATTGATC GCCCTAAAGG ACTGGCATTC 
ACTGATGTGG ATGTCGATTC CATCAAAATT GCTTGGGAAA GCCCACAGGG GCAAGTTTCC 
AGGTACAGGG TGACCTACTC GAGCCCTGAG GATGGAATCC ATGAGCTATT CCCTGCACCT 
GATGGTGAAG AAGACACTGC AGAGCTGCAA GGCCTCAGAC CGGGTTCTGA GTACACAGTC 
AGTGTGGTTG CCTTGCACGA TGATATGGAG AGCCAGCCCC TGATTGGAAC CCAGTCCACA 
GCTATTCCTG CACCAACTGA CCTGAAGTTC ACTCAGGTCA CACCCACAAG CCTGAGCGCC 
CAGTGGACAC CACCCAATGT TCAGCTCACT GGATATCGAG TGCGGGTGAC CCCCAAGGAG 
AAGACCGGAC CAATGAAAGA AATCAACCTT GCTCCTGACA GCTCATCCGT GGTTGTATCA 
GGACTTATGG TGGCCACCAA ATATGAAGTG AGTGTCTATG CTCTTAAGGA CACTTTGACA 
AGCAGACCAG CTCAGGGAGT TGTCACCACT CTGGAGAATG TCAGCCCACC AAGAAGGGCT 
CGTGTGACAG ATGCTACTGA GACCACCATC ACCATTAGCT GGAGAACCAA GACTGAGACG 
ATCACTGGCT TCCAAGTTGA TGCCGTTCCA GCCAATGGCC AGACTCCAAT CCAGAGAACC 
ATCAAGCCAG ATGTCAGAAG CTACACCATC ACAGGTTTAC AACCAGGCAC TGACTACAAG 
ATCTACCTGT ACACCTTGAA TGACAATGCT CGGAGCTCCC CTGTGGTCAT CGACGCCTCC 
ACTGCCATTG ATGCACCATC CAACCTGCGT TTCCTGGCCA CCACACCCAA TTCCTTGCTG 
GTATCATGGC AGCCGCCACG TGCCAGGATT ACCGGCTACA TCATCAAGTA TGAGAAGCCT 
GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG CCCCGCCCTG GTGTCACAGA GGCTACTATT 
ACTGGCCTGG AACCGGGAAC CGAATATACA ATTTATGTCA TTGCCCTGAA GAATAATCAG 
AAGAGCGAGC CCCTGATTGG AAGGAAAAAG ACAGACGAGC TTCCCCAACT GGTAACCCTT 
CCACACCCCA ATCTTCATGG ACCAGAGATC TTGGATGTTC CTTCCACAGT TCAAAAGACC 
CCTTTCGTCA CCCACCCTGG GTATGACACT GGAAATGGTA TTCAGCTTCC TGGCACTTCT 
GGTCAGCAAC CCAGTGTTGG GCAACAAATG ATCTTTGAGG AACATGGTTT TAGGCGGACC 
ACACCGCCCA CAACGGCCAC CCCCATAAGG CATAGGCCAA GACCATACCC GCCGAATGTA 
GGACAAGAAG CTCTCTCTCA GACAACCATC TCATGGGCCC CATTCCAGGA CACTTCTGAG 
TACATCATTT CATGTCATCC TGTTGGCACT GATGAAGAAC CCTTACAGTT CAGGGTTCCT 
GGAACTTCTA CCAGTGCCAC TCTGACAGGC CTCACCAGAG GTGCCACCTA CAACATCATA 
GTGGAGGCAC TGAAAGACCA GCAGAGGCAT AAGGTTCGGG AAGAGGTTGT TACCGTGGGC 
AACTCTGTCA ACGAAGGCTT GAACCAACCT ACGGATGACT CGTGCTTTGA CCCCTACACA 
GTTTCCCATT ATGCCGTTGG AGATGAGTGG GAACGAATGT CTGAATCAGG CTTTAAACTG 
TTGTGCCAGT GCTTAGGCTT TGGAAGTGGT CATTTCAGAT GTGATTCATC TAGATGGTGC 
CATGACAATG GTGTGAACTA CAAGATTGGA GAGAAGTGGG ACCGTCAGGG AGAAAATGGC 
CAGATGATGA GCTGCACATG TCTTGGGAAC GGAAAAGGAG AATTCAAGTG TGACCCTCAT 
GAGGCAACGT GTTATGATGA TGGGAAGACA TACCACGTAG GAGAACAGTG GCAGAAGGAA 
TATCTCGGTG CCATTTGCTC CTGCACATGC TTTGGAGGCC AGCGGGGCTG GCGCTGTGAC 
AACTGCCGCA GACCTGGGGG TGAACCCAGT CCCGAAGGCA CTACTGGCCA GTCCTACAAC 
CAGTATTCTC AGAGATACCA TCAGAGAACA AACACTAATG TTAATTGCCC AATTGAGTGC 
TTCATGCCTT TAGATGTACA GGCTGACAGA GAAGATTCCC GAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( NM_212478.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_997643.1 Homo sapiens fibronectin 1 (FN1), transcript variant 4, mRNA.

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CloneID	OHu25016
Clone ID Related Accession (Same CDS sequence)	NM_212478.2
Accession Version	NM_212478.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Homo sapiens(human)
Product	fibronectin isoform 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX640802.1, AJ535086.1, X02761.1, AI033037.1, U60068.1, BQ005645.1 and CR749317.1. On Jan 9, 2016 this sequence version replaced NM_212478.1. Transcript Variant: This variant (4) lacks an in-frame exon and two in-frame segments in the coding region, as compared to variant 1. Isoform 4 encoded by this variant thus lacks three internal segments, as compared to isoform 1. The encoded isoform (4) may undergo proteolytic processing similar to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.149860.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA 
GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG 
CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC 
AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT 
GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC 
CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT 
CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA 
CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA 
GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG 
ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC 
CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC 
ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG 
TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT 
GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA 
GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC 
AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT 
GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA 
GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG 
AGTCAGCCTC TGGTTCAGAC TGCAGTAACC AACATTGATC GCCCTAAAGG ACTGGCATTC 
ACTGATGTGG ATGTCGATTC CATCAAAATT GCTTGGGAAA GCCCACAGGG GCAAGTTTCC 
AGGTACAGGG TGACCTACTC GAGCCCTGAG GATGGAATCC ATGAGCTATT CCCTGCACCT 
GATGGTGAAG AAGACACTGC AGAGCTGCAA GGCCTCAGAC CGGGTTCTGA GTACACAGTC 
AGTGTGGTTG CCTTGCACGA TGATATGGAG AGCCAGCCCC TGATTGGAAC CCAGTCCACA 
GCTATTCCTG CACCAACTGA CCTGAAGTTC ACTCAGGTCA CACCCACAAG CCTGAGCGCC 
CAGTGGACAC CACCCAATGT TCAGCTCACT GGATATCGAG TGCGGGTGAC CCCCAAGGAG 
AAGACCGGAC CAATGAAAGA AATCAACCTT GCTCCTGACA GCTCATCCGT GGTTGTATCA 
GGACTTATGG TGGCCACCAA ATATGAAGTG AGTGTCTATG CTCTTAAGGA CACTTTGACA 
AGCAGACCAG CTCAGGGAGT TGTCACCACT CTGGAGAATG TCAGCCCACC AAGAAGGGCT 
CGTGTGACAG ATGCTACTGA GACCACCATC ACCATTAGCT GGAGAACCAA GACTGAGACG 
ATCACTGGCT TCCAAGTTGA TGCCGTTCCA GCCAATGGCC AGACTCCAAT CCAGAGAACC 
ATCAAGCCAG ATGTCAGAAG CTACACCATC ACAGGTTTAC AACCAGGCAC TGACTACAAG 
ATCTACCTGT ACACCTTGAA TGACAATGCT CGGAGCTCCC CTGTGGTCAT CGACGCCTCC 
ACTGCCATTG ATGCACCATC CAACCTGCGT TTCCTGGCCA CCACACCCAA TTCCTTGCTG 
GTATCATGGC AGCCGCCACG TGCCAGGATT ACCGGCTACA TCATCAAGTA TGAGAAGCCT 
GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG CCCCGCCCTG GTGTCACAGA GGCTACTATT 
ACTGGCCTGG AACCGGGAAC CGAATATACA ATTTATGTCA TTGCCCTGAA GAATAATCAG 
AAGAGCGAGC CCCTGATTGG AAGGAAAAAG ACAGTTCAAA AGACCCCTTT CGTCACCCAC 
CCTGGGTATG ACACTGGAAA TGGTATTCAG CTTCCTGGCA CTTCTGGTCA GCAACCCAGT 
GTTGGGCAAC AAATGATCTT TGAGGAACAT GGTTTTAGGC GGACCACACC GCCCACAACG 
GCCACCCCCA TAAGGCATAG GCCAAGACCA TACCCGCCGA ATGTAGGACA AGAAGCTCTC 
TCTCAGACAA CCATCTCATG GGCCCCATTC CAGGACACTT CTGAGTACAT CATTTCATGT 
CATCCTGTTG GCACTGATGA AGAACCCTTA CAGTTCAGGG TTCCTGGAAC TTCTACCAGT 
GCCACTCTGA CAGGCCTCAC CAGAGGTGCC ACCTACAACA TCATAGTGGA GGCACTGAAA 
GACCAGCAGA GGCATAAGGT TCGGGAAGAG GTTGTTACCG TGGGCAACTC TGTCAACGAA 
GGCTTGAACC AACCTACGGA TGACTCGTGC TTTGACCCCT ACACAGTTTC CCATTATGCC 
GTTGGAGATG AGTGGGAACG AATGTCTGAA TCAGGCTTTA AACTGTTGTG CCAGTGCTTA 
GGCTTTGGAA GTGGTCATTT CAGATGTGAT TCATCTAGAT GGTGCCATGA CAATGGTGTG 
AACTACAAGA TTGGAGAGAA GTGGGACCGT CAGGGAGAAA ATGGCCAGAT GATGAGCTGC 
ACATGTCTTG GGAACGGAAA AGGAGAATTC AAGTGTGACC CTCATGAGGC AACGTGTTAT 
GATGATGGGA AGACATACCA CGTAGGAGAA CAGTGGCAGA AGGAATATCT CGGTGCCATT 
TGCTCCTGCA CATGCTTTGG AGGCCAGCGG GGCTGGCGCT GTGACAACTG CCGCAGACCT 
GGGGGTGAAC CCAGTCCCGA AGGCACTACT GGCCAGTCCT ACAACCAGTA TTCTCAGAGA 
TACCATCAGA GAACAAACAC TAATGTTAAT TGCCCAATTG AGTGCTTCAT GCCTTTAGAT 
GTACAGGCTG ACAGAGAAGA TTCCCGAGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997643.1
CDS	267..7259
Translation	MLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDPTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIPAVPPPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSGESQPLVQTAVTNIDRPKGLAFTDVDVDSIKIAWESPQGQVSRYRVTYSSPEDGIHELFPAPDGEEDTAELQGLRPGSEYTVSVVALHDDMESQPLIGTQSTAIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTVQKTPFVTHPGYDTGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPIRHRPRPYPPNVGQEALSQTTISWAPFQDTSEYIISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGATYNIIVEALKDQQRHKVREEVVTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWERMSESGFKLLCQCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVNCPIECFMPLDVQADREDSRE

Target ORF information:

RefSeq Version	NM_212478.2
Organism	Homo sapiens(human)
Definition	Homo sapiens fibronectin 1 (FN1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212478.2

ORF Insert Sequence:

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA 
GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG 
CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC 
AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT 
GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC 
CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT 
CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA 
CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA 
GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG 
ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC 
CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC 
ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG 
TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT 
GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA 
GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC 
AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT 
GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA 
GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG 
AGTCAGCCTC TGGTTCAGAC TGCAGTAACC AACATTGATC GCCCTAAAGG ACTGGCATTC 
ACTGATGTGG ATGTCGATTC CATCAAAATT GCTTGGGAAA GCCCACAGGG GCAAGTTTCC 
AGGTACAGGG TGACCTACTC GAGCCCTGAG GATGGAATCC ATGAGCTATT CCCTGCACCT 
GATGGTGAAG AAGACACTGC AGAGCTGCAA GGCCTCAGAC CGGGTTCTGA GTACACAGTC 
AGTGTGGTTG CCTTGCACGA TGATATGGAG AGCCAGCCCC TGATTGGAAC CCAGTCCACA 
GCTATTCCTG CACCAACTGA CCTGAAGTTC ACTCAGGTCA CACCCACAAG CCTGAGCGCC 
CAGTGGACAC CACCCAATGT TCAGCTCACT GGATATCGAG TGCGGGTGAC CCCCAAGGAG 
AAGACCGGAC CAATGAAAGA AATCAACCTT GCTCCTGACA GCTCATCCGT GGTTGTATCA 
GGACTTATGG TGGCCACCAA ATATGAAGTG AGTGTCTATG CTCTTAAGGA CACTTTGACA 
AGCAGACCAG CTCAGGGAGT TGTCACCACT CTGGAGAATG TCAGCCCACC AAGAAGGGCT 
CGTGTGACAG ATGCTACTGA GACCACCATC ACCATTAGCT GGAGAACCAA GACTGAGACG 
ATCACTGGCT TCCAAGTTGA TGCCGTTCCA GCCAATGGCC AGACTCCAAT CCAGAGAACC 
ATCAAGCCAG ATGTCAGAAG CTACACCATC ACAGGTTTAC AACCAGGCAC TGACTACAAG 
ATCTACCTGT ACACCTTGAA TGACAATGCT CGGAGCTCCC CTGTGGTCAT CGACGCCTCC 
ACTGCCATTG ATGCACCATC CAACCTGCGT TTCCTGGCCA CCACACCCAA TTCCTTGCTG 
GTATCATGGC AGCCGCCACG TGCCAGGATT ACCGGCTACA TCATCAAGTA TGAGAAGCCT 
GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG CCCCGCCCTG GTGTCACAGA GGCTACTATT 
ACTGGCCTGG AACCGGGAAC CGAATATACA ATTTATGTCA TTGCCCTGAA GAATAATCAG 
AAGAGCGAGC CCCTGATTGG AAGGAAAAAG ACAGTTCAAA AGACCCCTTT CGTCACCCAC 
CCTGGGTATG ACACTGGAAA TGGTATTCAG CTTCCTGGCA CTTCTGGTCA GCAACCCAGT 
GTTGGGCAAC AAATGATCTT TGAGGAACAT GGTTTTAGGC GGACCACACC GCCCACAACG 
GCCACCCCCA TAAGGCATAG GCCAAGACCA TACCCGCCGA ATGTAGGACA AGAAGCTCTC 
TCTCAGACAA CCATCTCATG GGCCCCATTC CAGGACACTT CTGAGTACAT CATTTCATGT 
CATCCTGTTG GCACTGATGA AGAACCCTTA CAGTTCAGGG TTCCTGGAAC TTCTACCAGT 
GCCACTCTGA CAGGCCTCAC CAGAGGTGCC ACCTACAACA TCATAGTGGA GGCACTGAAA 
GACCAGCAGA GGCATAAGGT TCGGGAAGAG GTTGTTACCG TGGGCAACTC TGTCAACGAA 
GGCTTGAACC AACCTACGGA TGACTCGTGC TTTGACCCCT ACACAGTTTC CCATTATGCC 
GTTGGAGATG AGTGGGAACG AATGTCTGAA TCAGGCTTTA AACTGTTGTG CCAGTGCTTA 
GGCTTTGGAA GTGGTCATTT CAGATGTGAT TCATCTAGAT GGTGCCATGA CAATGGTGTG 
AACTACAAGA TTGGAGAGAA GTGGGACCGT CAGGGAGAAA ATGGCCAGAT GATGAGCTGC 
ACATGTCTTG GGAACGGAAA AGGAGAATTC AAGTGTGACC CTCATGAGGC AACGTGTTAT 
GATGATGGGA AGACATACCA CGTAGGAGAA CAGTGGCAGA AGGAATATCT CGGTGCCATT 
TGCTCCTGCA CATGCTTTGG AGGCCAGCGG GGCTGGCGCT GTGACAACTG CCGCAGACCT 
GGGGGTGAAC CCAGTCCCGA AGGCACTACT GGCCAGTCCT ACAACCAGTA TTCTCAGAGA 
TACCATCAGA GAACAAACAC TAATGTTAAT TGCCCAATTG AGTGCTTCAT GCCTTTAGAT 
GTACAGGCTG ACAGAGAAGA TTCCCGAGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( NM_001365517.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001352446.1 Homo sapiens fibronectin 1 (FN1), transcript variant 12, mRNA.

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CloneID	OHu36020
Clone ID Related Accession (Same CDS sequence)	XM_005246405.1 , NM_001365517.1
Accession Version	NM_001365517.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	fibronectin isoform 12 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012462.5 and AC073284.5. On Aug 30, 2018 this sequence version replaced XM_005246405.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.254677.1, SRR1803611.47131.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG 
TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG 
ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA 
GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG 
TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG 
CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC 
GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT 
AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG 
AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC 
CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC 
AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT 
GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG 
GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC 
TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA 
GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT 
GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG 
AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT 
GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG 
CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA 
TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG 
CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG 
AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG 
TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA 
GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG 
CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC 
AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT 
CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG 
GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT 
TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC 
CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG 
TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA 
ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC 
TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC 
ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC 
GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT 
GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC 
TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG 
AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC 
ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT 
CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG 
CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT 
GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA 
GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG 
ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA 
CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG 
GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG 
CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT 
TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT 
CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT 
CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA 
CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA 
GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT 
CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT 
ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT 
CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT 
GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT 
GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA 
CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA 
AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA 
ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC 
GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG 
GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA 
TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG 
ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG 
GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG 
ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA 
ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC 
AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC 
TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG 
CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG 
CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT 
ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT 
CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGACG AGCTTCCCCA ACTGGTAACC 
CTTCCACACC CCAATCTTCA TGGACCAGAG ATCTTGGATG TTCCTTCCAC AGTTCAAAAG 
ACCCCTTTCG TCACCCACCC TGGGTATGAC ACTGGAAATG GTATTCAGCT TCCTGGCACT 
TCTGGTCAGC AACCCAGTGT TGGGCAACAA ATGATCTTTG AGGAACATGG TTTTAGGCGG 
ACCACACCGC CCACAACGGC CACCCCCATA AGGCATAGGC CAAGACCATA CCCGCCGAAT 
GTAGGTGAGG AAATCCAAAT TGGTCACATC CCCAGGGAAG ATGTAGACTA TCACCTGTAC 
CCACACGGTC CGGGACTCAA TCCAAATGCC TCTACAGGAC AAGAAGCTCT CTCTCAGACA 
ACCATCTCAT GGGCCCCATT CCAGGACACT TCTGAGTACA TCATTTCATG TCATCCTGTT 
GGCACTGATG AAGAACCCTT ACAGTTCAGG GTTCCTGGAA CTTCTACCAG TGCCACTCTG 
ACAGGCCTCA CCAGAGGTGC CACCTACAAC GTCATAGTGG AGGCACTGAA AGACCAGCAG 
AGGCATAAGG TTCGGGAAGA GGTTGTTACC GTGGGCAACT CTGTCAACGA AGGCTTGAAC 
CAACCTACGG ATGACTCGTG CTTTGACCCC TACACAGTTT CCCATTATGC CGTTGGAGAT 
GAGTGGGAAC GAATGTCTGA ATCAGGCTTT AAACTGTTGT GCCAGTGCTT AGGCTTTGGA 
AGTGGTCATT TCAGATGTGA TTCATCTAGA TGGTGCCATG ACAATGGTGT GAACTACAAG 
ATTGGAGAGA AGTGGGACCG TCAGGGAGAA AATGGCCAGA TGATGAGCTG CACATGTCTT 
GGGAACGGAA AAGGAGAATT CAAGTGTGAC CCTCATGAGG CAACGTGTTA TGATGATGGG 
AAGACATACC ACGTAGGAGA ACAGTGGCAG AAGGAATATC TCGGTGCCAT TTGCTCCTGC 
ACATGCTTTG GAGGCCAGCG GGGCTGGCGC TGTGACAACT GCCGCAGACC TGGGGGTGAA 
CCCAGTCCCG AAGGCACTAC TGGCCAGTCC TACAACCAGT ATTCTCAGAG ATACCATCAG 
AGAACAAACA CTAATGTTAA TTGCCCAATT GAGTGCTTCA TGCCTTTAGA TGTACAGGCT 
GACAGAGAAG ATTCCCGAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352446.1
CDS	267..7430
Translation	MLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSGESQPLVQTAVTTIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPSTVQKTPFVTHPGYDTGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPIRHRPRPYPPNVGEEIQIGHIPREDVDYHLYPHGPGLNPNASTGQEALSQTTISWAPFQDTSEYIISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGATYNVIVEALKDQQRHKVREEVVTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWERMSESGFKLLCQCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVNCPIECFMPLDVQADREDSRE

FN1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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