FN1 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following FN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu24787 NM_212482.3
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$659.50-$923.30
$1319.00
OHu24809 NM_054034.2
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu107507 NM_001365519.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant 14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OHu89216 NM_001365521.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant 16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OHu89217 NM_001365522.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant 17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHu104833 XM_024452769.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OHu104834 XM_024452770.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OHu89215 XM_017003692.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OHu89216 XM_017003693.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OHu89217 XM_017003694.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHu89218 XM_017003695.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHu24915 NM_212476.2
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $923.30
$1319.00
OHu24997 NM_212474.2
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $923.30
$1319.00
OHu25005 NM_002026.3
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu25016 NM_212478.2
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $923.30
$1319.00
OHu36020 NM_001365517.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant 12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu36021 NM_001365518.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant 13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu36024 NM_001365520.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant 15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu36027 NM_001365523.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant 18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu36029 NM_001365524.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant 19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu57835 NM_001306131.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu57836 NM_001306129.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu57837 NM_001306130.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu57838 NM_001306132.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant 11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu36012 XM_005246397.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu36013 XM_005246398.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu36014 XM_005246399.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu36016 XM_005246401.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu36017 XM_005246402.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu36018 XM_005246403.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu36019 XM_005246404.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu36020 XM_005246405.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu36021 XM_005246406.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu36022 XM_005246407.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu36023 XM_005246408.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu36024 XM_005246409.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu36025 XM_005246410.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu36026 XM_005246411.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu36027 XM_005246412.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu36029 XM_005246414.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu36031 XM_005246416.1
Latest version!
Homo sapiens fibronectin 1 (FN1), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
View Old Accession Versions >>
OHu24787 NM_212482.1 Homo sapiens fibronectin 1 (FN1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$659.50-$923.30
$1319.00
OHu24787 NM_212482.2 Homo sapiens fibronectin 1 (FN1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$659.50-$923.30
$1319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu24787
    Clone ID Related Accession (Same CDS sequence) NM_212482.3 , NM_212482.1 , NM_212482.2
    Accession Version NM_212482.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7434bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product fibronectin isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX640803.1, AJ535086.1, BX640875.1, AI033037.1, U60068.1, BX640999.1, BQ005645.1 and CR749317.1. On Nov 22, 2018 this sequence version replaced NM_212482.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). The encoded isoform (1) may undergo proteolytic processing similar to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX640803.1, BX640875.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA
    ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT
    GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG
    CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG
    GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG
    TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC
    ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC
    CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC
    ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT
    CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC
    ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT
    GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG
    ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT
    ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA
    AAGACAGACG AGCTTCCCCA ACTGGTAACC CTTCCACACC CCAATCTTCA TGGACCAGAG
    ATCTTGGATG TTCCTTCCAC AGTTCAAAAG ACCCCTTTCG TCACCCACCC TGGGTATGAC
    ACTGGAAATG GTATTCAGCT TCCTGGCACT TCTGGTCAGC AACCCAGTGT TGGGCAACAA
    ATGATCTTTG AGGAACATGG TTTTAGGCGG ACCACACCGC CCACAACGGC CACCCCCATA
    AGGCATAGGC CAAGACCATA CCCGCCGAAT GTAGGTGAGG AAATCCAAAT TGGTCACATC
    CCCAGGGAAG ATGTAGACTA TCACCTGTAC CCACACGGTC CGGGACTCAA TCCAAATGCC
    TCTACAGGAC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGGCCCCATT CCAGGACACT
    TCTGAGTACA TCATTTCATG TCATCCTGTT GGCACTGATG AAGAACCCTT ACAGTTCAGG
    GTTCCTGGAA CTTCTACCAG TGCCACTCTG ACAGGCCTCA CCAGAGGTGC CACCTACAAC
    ATCATAGTGG AGGCACTGAA AGACCAGCAG AGGCATAAGG TTCGGGAAGA GGTTGTTACC
    GTGGGCAACT CTGTCAACGA AGGCTTGAAC CAACCTACGG ATGACTCGTG CTTTGACCCC
    TACACAGTTT CCCATTATGC CGTTGGAGAT GAGTGGGAAC GAATGTCTGA ATCAGGCTTT
    AAACTGTTGT GCCAGTGCTT AGGCTTTGGA AGTGGTCATT TCAGATGTGA TTCATCTAGA
    TGGTGCCATG ACAATGGTGT GAACTACAAG ATTGGAGAGA AGTGGGACCG TCAGGGAGAA
    AATGGCCAGA TGATGAGCTG CACATGTCTT GGGAACGGAA AAGGAGAATT CAAGTGTGAC
    CCTCATGAGG CAACGTGTTA TGATGATGGG AAGACATACC ACGTAGGAGA ACAGTGGCAG
    AAGGAATATC TCGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC
    TGTGACAACT GCCGCAGACC TGGGGGTGAA CCCAGTCCCG AAGGCACTAC TGGCCAGTCC
    TACAACCAGT ATTCTCAGAG ATACCATCAG AGAACAAACA CTAATGTTAA TTGCCCAATT
    GAGTGCTTCA TGCCTTTAGA TGTACAGGCT GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_997647.1
    CDS267..7700
    Translation

    Target ORF information:

    RefSeq Version NM_212482.3
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_212482.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA
    ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT
    GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG
    CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG
    GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG
    TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC
    ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC
    CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC
    ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT
    CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC
    ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT
    GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG
    ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT
    ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA
    AAGACAGACG AGCTTCCCCA ACTGGTAACC CTTCCACACC CCAATCTTCA TGGACCAGAG
    ATCTTGGATG TTCCTTCCAC AGTTCAAAAG ACCCCTTTCG TCACCCACCC TGGGTATGAC
    ACTGGAAATG GTATTCAGCT TCCTGGCACT TCTGGTCAGC AACCCAGTGT TGGGCAACAA
    ATGATCTTTG AGGAACATGG TTTTAGGCGG ACCACACCGC CCACAACGGC CACCCCCATA
    AGGCATAGGC CAAGACCATA CCCGCCGAAT GTAGGTGAGG AAATCCAAAT TGGTCACATC
    CCCAGGGAAG ATGTAGACTA TCACCTGTAC CCACACGGTC CGGGACTCAA TCCAAATGCC
    TCTACAGGAC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGGCCCCATT CCAGGACACT
    TCTGAGTACA TCATTTCATG TCATCCTGTT GGCACTGATG AAGAACCCTT ACAGTTCAGG
    GTTCCTGGAA CTTCTACCAG TGCCACTCTG ACAGGCCTCA CCAGAGGTGC CACCTACAAC
    ATCATAGTGG AGGCACTGAA AGACCAGCAG AGGCATAAGG TTCGGGAAGA GGTTGTTACC
    GTGGGCAACT CTGTCAACGA AGGCTTGAAC CAACCTACGG ATGACTCGTG CTTTGACCCC
    TACACAGTTT CCCATTATGC CGTTGGAGAT GAGTGGGAAC GAATGTCTGA ATCAGGCTTT
    AAACTGTTGT GCCAGTGCTT AGGCTTTGGA AGTGGTCATT TCAGATGTGA TTCATCTAGA
    TGGTGCCATG ACAATGGTGT GAACTACAAG ATTGGAGAGA AGTGGGACCG TCAGGGAGAA
    AATGGCCAGA TGATGAGCTG CACATGTCTT GGGAACGGAA AAGGAGAATT CAAGTGTGAC
    CCTCATGAGG CAACGTGTTA TGATGATGGG AAGACATACC ACGTAGGAGA ACAGTGGCAG
    AAGGAATATC TCGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC
    TGTGACAACT GCCGCAGACC TGGGGGTGAA CCCAGTCCCG AAGGCACTAC TGGCCAGTCC
    TACAACCAGT ATTCTCAGAG ATACCATCAG AGAACAAACA CTAATGTTAA TTGCCCAATT
    GAGTGCTTCA TGCCTTTAGA TGTACAGGCT GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24787
    Clone ID Related Accession (Same CDS sequence) NM_212482.3 , NM_212482.1 , NM_212482.2
    Accession Version NM_212482.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7434bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-04-14
    Organism Homo sapiens(Human)
    Product fibronectin isoform 1 preproprotein
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA
    ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT
    GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG
    CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG
    GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG
    TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC
    ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC
    CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC
    ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT
    CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC
    ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT
    GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG
    ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT
    ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA
    AAGACAGACG AGCTTCCCCA ACTGGTAACC CTTCCACACC CCAATCTTCA TGGACCAGAG
    ATCTTGGATG TTCCTTCCAC AGTTCAAAAG ACCCCTTTCG TCACCCACCC TGGGTATGAC
    ACTGGAAATG GTATTCAGCT TCCTGGCACT TCTGGTCAGC AACCCAGTGT TGGGCAACAA
    ATGATCTTTG AGGAACATGG TTTTAGGCGG ACCACACCGC CCACAACGGC CACCCCCATA
    AGGCATAGGC CAAGACCATA CCCGCCGAAT GTAGGTGAGG AAATCCAAAT TGGTCACATC
    CCCAGGGAAG ATGTAGACTA TCACCTGTAC CCACACGGTC CGGGACTCAA TCCAAATGCC
    TCTACAGGAC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGGCCCCATT CCAGGACACT
    TCTGAGTACA TCATTTCATG TCATCCTGTT GGCACTGATG AAGAACCCTT ACAGTTCAGG
    GTTCCTGGAA CTTCTACCAG TGCCACTCTG ACAGGCCTCA CCAGAGGTGC CACCTACAAC
    ATCATAGTGG AGGCACTGAA AGACCAGCAG AGGCATAAGG TTCGGGAAGA GGTTGTTACC
    GTGGGCAACT CTGTCAACGA AGGCTTGAAC CAACCTACGG ATGACTCGTG CTTTGACCCC
    TACACAGTTT CCCATTATGC CGTTGGAGAT GAGTGGGAAC GAATGTCTGA ATCAGGCTTT
    AAACTGTTGT GCCAGTGCTT AGGCTTTGGA AGTGGTCATT TCAGATGTGA TTCATCTAGA
    TGGTGCCATG ACAATGGTGT GAACTACAAG ATTGGAGAGA AGTGGGACCG TCAGGGAGAA
    AATGGCCAGA TGATGAGCTG CACATGTCTT GGGAACGGAA AAGGAGAATT CAAGTGTGAC
    CCTCATGAGG CAACGTGTTA TGATGATGGG AAGACATACC ACGTAGGAGA ACAGTGGCAG
    AAGGAATATC TCGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC
    TGTGACAACT GCCGCAGACC TGGGGGTGAA CCCAGTCCCG AAGGCACTAC TGGCCAGTCC
    TACAACCAGT ATTCTCAGAG ATACCATCAG AGAACAAACA CTAATGTTAA TTGCCCAATT
    GAGTGCTTCA TGCCTTTAGA TGTACAGGCT GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_997647.1
    Translation

    Target ORF information:

    RefSeq Version NM_212482.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_212482.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA
    ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT
    GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG
    CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG
    GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG
    TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC
    ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC
    CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC
    ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT
    CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC
    ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT
    GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG
    ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT
    ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA
    AAGACAGACG AGCTTCCCCA ACTGGTAACC CTTCCACACC CCAATCTTCA TGGACCAGAG
    ATCTTGGATG TTCCTTCCAC AGTTCAAAAG ACCCCTTTCG TCACCCACCC TGGGTATGAC
    ACTGGAAATG GTATTCAGCT TCCTGGCACT TCTGGTCAGC AACCCAGTGT TGGGCAACAA
    ATGATCTTTG AGGAACATGG TTTTAGGCGG ACCACACCGC CCACAACGGC CACCCCCATA
    AGGCATAGGC CAAGACCATA CCCGCCGAAT GTAGGTGAGG AAATCCAAAT TGGTCACATC
    CCCAGGGAAG ATGTAGACTA TCACCTGTAC CCACACGGTC CGGGACTCAA TCCAAATGCC
    TCTACAGGAC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGGCCCCATT CCAGGACACT
    TCTGAGTACA TCATTTCATG TCATCCTGTT GGCACTGATG AAGAACCCTT ACAGTTCAGG
    GTTCCTGGAA CTTCTACCAG TGCCACTCTG ACAGGCCTCA CCAGAGGTGC CACCTACAAC
    ATCATAGTGG AGGCACTGAA AGACCAGCAG AGGCATAAGG TTCGGGAAGA GGTTGTTACC
    GTGGGCAACT CTGTCAACGA AGGCTTGAAC CAACCTACGG ATGACTCGTG CTTTGACCCC
    TACACAGTTT CCCATTATGC CGTTGGAGAT GAGTGGGAAC GAATGTCTGA ATCAGGCTTT
    AAACTGTTGT GCCAGTGCTT AGGCTTTGGA AGTGGTCATT TCAGATGTGA TTCATCTAGA
    TGGTGCCATG ACAATGGTGT GAACTACAAG ATTGGAGAGA AGTGGGACCG TCAGGGAGAA
    AATGGCCAGA TGATGAGCTG CACATGTCTT GGGAACGGAA AAGGAGAATT CAAGTGTGAC
    CCTCATGAGG CAACGTGTTA TGATGATGGG AAGACATACC ACGTAGGAGA ACAGTGGCAG
    AAGGAATATC TCGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC
    TGTGACAACT GCCGCAGACC TGGGGGTGAA CCCAGTCCCG AAGGCACTAC TGGCCAGTCC
    TACAACCAGT ATTCTCAGAG ATACCATCAG AGAACAAACA CTAATGTTAA TTGCCCAATT
    GAGTGCTTCA TGCCTTTAGA TGTACAGGCT GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24787
    Clone ID Related Accession (Same CDS sequence) NM_212482.3 , NM_212482.1 , NM_212482.2
    Accession Version NM_212482.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7434bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-17
    Organism Homo sapiens(human)
    Product fibronectin isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX640803.1, AJ535086.1, BX640875.1, AI033037.1, U60068.1, BX640999.1, BQ005645.1 and CR749317.1. This sequence is a reference standard in the RefSeqGene project. On Jan 9, 2016 this sequence version replaced NM_212482.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). The encoded isoform (1) may undergo proteolytic processing similar to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX640803.1, BX640875.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA
    ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT
    GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG
    CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG
    GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG
    TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC
    ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC
    CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC
    ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT
    CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC
    ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT
    GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG
    ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT
    ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA
    AAGACAGACG AGCTTCCCCA ACTGGTAACC CTTCCACACC CCAATCTTCA TGGACCAGAG
    ATCTTGGATG TTCCTTCCAC AGTTCAAAAG ACCCCTTTCG TCACCCACCC TGGGTATGAC
    ACTGGAAATG GTATTCAGCT TCCTGGCACT TCTGGTCAGC AACCCAGTGT TGGGCAACAA
    ATGATCTTTG AGGAACATGG TTTTAGGCGG ACCACACCGC CCACAACGGC CACCCCCATA
    AGGCATAGGC CAAGACCATA CCCGCCGAAT GTAGGTGAGG AAATCCAAAT TGGTCACATC
    CCCAGGGAAG ATGTAGACTA TCACCTGTAC CCACACGGTC CGGGACTCAA TCCAAATGCC
    TCTACAGGAC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGGCCCCATT CCAGGACACT
    TCTGAGTACA TCATTTCATG TCATCCTGTT GGCACTGATG AAGAACCCTT ACAGTTCAGG
    GTTCCTGGAA CTTCTACCAG TGCCACTCTG ACAGGCCTCA CCAGAGGTGC CACCTACAAC
    ATCATAGTGG AGGCACTGAA AGACCAGCAG AGGCATAAGG TTCGGGAAGA GGTTGTTACC
    GTGGGCAACT CTGTCAACGA AGGCTTGAAC CAACCTACGG ATGACTCGTG CTTTGACCCC
    TACACAGTTT CCCATTATGC CGTTGGAGAT GAGTGGGAAC GAATGTCTGA ATCAGGCTTT
    AAACTGTTGT GCCAGTGCTT AGGCTTTGGA AGTGGTCATT TCAGATGTGA TTCATCTAGA
    TGGTGCCATG ACAATGGTGT GAACTACAAG ATTGGAGAGA AGTGGGACCG TCAGGGAGAA
    AATGGCCAGA TGATGAGCTG CACATGTCTT GGGAACGGAA AAGGAGAATT CAAGTGTGAC
    CCTCATGAGG CAACGTGTTA TGATGATGGG AAGACATACC ACGTAGGAGA ACAGTGGCAG
    AAGGAATATC TCGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC
    TGTGACAACT GCCGCAGACC TGGGGGTGAA CCCAGTCCCG AAGGCACTAC TGGCCAGTCC
    TACAACCAGT ATTCTCAGAG ATACCATCAG AGAACAAACA CTAATGTTAA TTGCCCAATT
    GAGTGCTTCA TGCCTTTAGA TGTACAGGCT GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_997647.1
    CDS267..7700
    Translation

    Target ORF information:

    RefSeq Version NM_212482.2
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_212482.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA
    ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT
    GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG
    CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG
    GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG
    TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC
    ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC
    CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC
    ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT
    CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC
    ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT
    GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG
    ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT
    ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA
    AAGACAGACG AGCTTCCCCA ACTGGTAACC CTTCCACACC CCAATCTTCA TGGACCAGAG
    ATCTTGGATG TTCCTTCCAC AGTTCAAAAG ACCCCTTTCG TCACCCACCC TGGGTATGAC
    ACTGGAAATG GTATTCAGCT TCCTGGCACT TCTGGTCAGC AACCCAGTGT TGGGCAACAA
    ATGATCTTTG AGGAACATGG TTTTAGGCGG ACCACACCGC CCACAACGGC CACCCCCATA
    AGGCATAGGC CAAGACCATA CCCGCCGAAT GTAGGTGAGG AAATCCAAAT TGGTCACATC
    CCCAGGGAAG ATGTAGACTA TCACCTGTAC CCACACGGTC CGGGACTCAA TCCAAATGCC
    TCTACAGGAC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGGCCCCATT CCAGGACACT
    TCTGAGTACA TCATTTCATG TCATCCTGTT GGCACTGATG AAGAACCCTT ACAGTTCAGG
    GTTCCTGGAA CTTCTACCAG TGCCACTCTG ACAGGCCTCA CCAGAGGTGC CACCTACAAC
    ATCATAGTGG AGGCACTGAA AGACCAGCAG AGGCATAAGG TTCGGGAAGA GGTTGTTACC
    GTGGGCAACT CTGTCAACGA AGGCTTGAAC CAACCTACGG ATGACTCGTG CTTTGACCCC
    TACACAGTTT CCCATTATGC CGTTGGAGAT GAGTGGGAAC GAATGTCTGA ATCAGGCTTT
    AAACTGTTGT GCCAGTGCTT AGGCTTTGGA AGTGGTCATT TCAGATGTGA TTCATCTAGA
    TGGTGCCATG ACAATGGTGT GAACTACAAG ATTGGAGAGA AGTGGGACCG TCAGGGAGAA
    AATGGCCAGA TGATGAGCTG CACATGTCTT GGGAACGGAA AAGGAGAATT CAAGTGTGAC
    CCTCATGAGG CAACGTGTTA TGATGATGGG AAGACATACC ACGTAGGAGA ACAGTGGCAG
    AAGGAATATC TCGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC
    TGTGACAACT GCCGCAGACC TGGGGGTGAA CCCAGTCCCG AAGGCACTAC TGGCCAGTCC
    TACAACCAGT ATTCTCAGAG ATACCATCAG AGAACAAACA CTAATGTTAA TTGCCCAATT
    GAGTGCTTCA TGCCTTTAGA TGTACAGGCT GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24809
    Clone ID Related Accession (Same CDS sequence) NM_054034.2
    Accession Version NM_054034.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1974bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Homo sapiens(human)
    Product fibronectin isoform 7 preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX640608.1, AJ276395.1 and AJ535086.1. On May 12, 2004 this sequence version replaced NM_054034.1. Transcript Variant: This variant (7) lacks multiple 3' exons and has an alternate 3' end, as compared to variant 1. Isoform 7 encoded by this variant is much shorter and has a distinct C-terminus, as compared isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ535086.1, BX538045.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467147, SAMEA2467148 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TGTGAGTATC CCACCCAGAA ACCTTGGATA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_473375.2
    CDS267..2240
    Translation

    Target ORF information:

    RefSeq Version NM_054034.2
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_054034.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TGTGAGTATC CCACCCAGAA ACCTTGGATA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu107507
    Clone ID Related Accession (Same CDS sequence) NM_001365519.1
    Accession Version NM_001365519.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7089bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-29
    Organism Homo sapiens(human)
    Product fibronectin isoform 14 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012462.5 and AC073284.5. On Aug 30, 2018 this sequence version replaced XM_024452771.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.52979.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC
    GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG
    GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA
    TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG
    GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG
    ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA
    ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC
    AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG
    CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG
    CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT
    ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT
    CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGTTC AAAAGACCCC TTTCGTCACC
    CACCCTGGGT ATGACACTGG AAATGGTATT CAGCTTCCTG GCACTTCTGG TCAGCAACCC
    AGTGTTGGGC AACAAATGAT CTTTGAGGAA CATGGTTTTA GGCGGACCAC ACCGCCCACA
    ACGGCCACCC CCATAAGGCA TAGGCCAAGA CCATACCCGC CGAATGTAGG TGAGGAAATC
    CAAATTGGTC ACATCCCCAG GGAAGATGTA GACTATCACC TGTACCCACA CGGTCCGGGA
    CTCAATCCAA ATGCCTCTAC AGGACAAGAA GCTCTCTCTC AGACAACCAT CTCATGGGCC
    CCATTCCAGG ACACTTCTGA GTACATCATT TCATGTCATC CTGTTGGCAC TGATGAAGAA
    CCCTTACAGT TCAGGGTTCC TGGAACTTCT ACCAGTGCCA CTCTGACAGG CCTCACCAGA
    GGTGCCACCT ACAACGTCAT AGTGGAGGCA CTGAAAGACC AGCAGAGGCA TAAGGTTCGG
    GAAGAGGTTG TTACCGTGGG CAACTCTGTC AACGAAGGCT TGAACCAACC TACGGATGAC
    TCGTGCTTTG ACCCCTACAC AGTTTCCCAT TATGCCGTTG GAGATGAGTG GGAACGAATG
    TCTGAATCAG GCTTTAAACT GTTGTGCCAG TGCTTAGGCT TTGGAAGTGG TCATTTCAGA
    TGTGATTCAT CTAGATGGTG CCATGACAAT GGTGTGAACT ACAAGATTGG AGAGAAGTGG
    GACCGTCAGG GAGAAAATGG CCAGATGATG AGCTGCACAT GTCTTGGGAA CGGAAAAGGA
    GAATTCAAGT GTGACCCTCA TGAGGCAACG TGTTATGATG ATGGGAAGAC ATACCACGTA
    GGAGAACAGT GGCAGAAGGA ATATCTCGGT GCCATTTGCT CCTGCACATG CTTTGGAGGC
    CAGCGGGGCT GGCGCTGTGA CAACTGCCGC AGACCTGGGG GTGAACCCAG TCCCGAAGGC
    ACTACTGGCC AGTCCTACAA CCAGTATTCT CAGAGATACC ATCAGAGAAC AAACACTAAT
    GTTAATTGCC CAATTGAGTG CTTCATGCCT TTAGATGTAC AGGCTGACAG AGAAGATTCC
    CGAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001352448.1
    CDS267..7355
    Translation

    Target ORF information:

    RefSeq Version NM_001365519.1
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant 14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001365519.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC
    GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG
    GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA
    TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG
    GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG
    ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA
    ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC
    AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG
    CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG
    CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT
    ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT
    CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGTTC AAAAGACCCC TTTCGTCACC
    CACCCTGGGT ATGACACTGG AAATGGTATT CAGCTTCCTG GCACTTCTGG TCAGCAACCC
    AGTGTTGGGC AACAAATGAT CTTTGAGGAA CATGGTTTTA GGCGGACCAC ACCGCCCACA
    ACGGCCACCC CCATAAGGCA TAGGCCAAGA CCATACCCGC CGAATGTAGG TGAGGAAATC
    CAAATTGGTC ACATCCCCAG GGAAGATGTA GACTATCACC TGTACCCACA CGGTCCGGGA
    CTCAATCCAA ATGCCTCTAC AGGACAAGAA GCTCTCTCTC AGACAACCAT CTCATGGGCC
    CCATTCCAGG ACACTTCTGA GTACATCATT TCATGTCATC CTGTTGGCAC TGATGAAGAA
    CCCTTACAGT TCAGGGTTCC TGGAACTTCT ACCAGTGCCA CTCTGACAGG CCTCACCAGA
    GGTGCCACCT ACAACGTCAT AGTGGAGGCA CTGAAAGACC AGCAGAGGCA TAAGGTTCGG
    GAAGAGGTTG TTACCGTGGG CAACTCTGTC AACGAAGGCT TGAACCAACC TACGGATGAC
    TCGTGCTTTG ACCCCTACAC AGTTTCCCAT TATGCCGTTG GAGATGAGTG GGAACGAATG
    TCTGAATCAG GCTTTAAACT GTTGTGCCAG TGCTTAGGCT TTGGAAGTGG TCATTTCAGA
    TGTGATTCAT CTAGATGGTG CCATGACAAT GGTGTGAACT ACAAGATTGG AGAGAAGTGG
    GACCGTCAGG GAGAAAATGG CCAGATGATG AGCTGCACAT GTCTTGGGAA CGGAAAAGGA
    GAATTCAAGT GTGACCCTCA TGAGGCAACG TGTTATGATG ATGGGAAGAC ATACCACGTA
    GGAGAACAGT GGCAGAAGGA ATATCTCGGT GCCATTTGCT CCTGCACATG CTTTGGAGGC
    CAGCGGGGCT GGCGCTGTGA CAACTGCCGC AGACCTGGGG GTGAACCCAG TCCCGAAGGC
    ACTACTGGCC AGTCCTACAA CCAGTATTCT CAGAGATACC ATCAGAGAAC AAACACTAAT
    GTTAATTGCC CAATTGAGTG CTTCATGCCT TTAGATGTAC AGGCTGACAG AGAAGATTCC
    CGAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu89216
    Clone ID Related Accession (Same CDS sequence) XM_017003693.1 , NM_001365521.1
    Accession Version NM_001365521.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7071bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Homo sapiens(human)
    Product fibronectin isoform 16 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012462.5 and AC073284.5. On Aug 30, 2018 this sequence version replaced XM_017003693.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.226196.1, SRR1803613.233494.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

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    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
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    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC
    GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG
    GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA
    TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG
    GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG
    ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA
    ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC
    AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG
    CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG
    CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT
    ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT
    CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGACG AGCTTCCCCA ACTGGTAACC
    CTTCCACACC CCAATCTTCA TGGACCAGAG ATCTTGGATG TTCCTTCCAC AGTTCAAAAG
    ACCCCTTTCG TCACCCACCC TGGGTATGAC ACTGGAAATG GTATTCAGCT TCCTGGCACT
    TCTGGTCAGC AACCCAGTGT TGGGCAACAA ATGATCTTTG AGGAACATGG TTTTAGGCGG
    ACCACACCGC CCACAACGGC CACCCCCATA AGGCATAGGC CAAGACCATA CCCGCCGAAT
    GTAGGACAAG AAGCTCTCTC TCAGACAACC ATCTCATGGG CCCCATTCCA GGACACTTCT
    GAGTACATCA TTTCATGTCA TCCTGTTGGC ACTGATGAAG AACCCTTACA GTTCAGGGTT
    CCTGGAACTT CTACCAGTGC CACTCTGACA GGCCTCACCA GAGGTGCCAC CTACAACGTC
    ATAGTGGAGG CACTGAAAGA CCAGCAGAGG CATAAGGTTC GGGAAGAGGT TGTTACCGTG
    GGCAACTCTG TCAACGAAGG CTTGAACCAA CCTACGGATG ACTCGTGCTT TGACCCCTAC
    ACAGTTTCCC ATTATGCCGT TGGAGATGAG TGGGAACGAA TGTCTGAATC AGGCTTTAAA
    CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT GGTCATTTCA GATGTGATTC ATCTAGATGG
    TGCCATGACA ATGGTGTGAA CTACAAGATT GGAGAGAAGT GGGACCGTCA GGGAGAAAAT
    GGCCAGATGA TGAGCTGCAC ATGTCTTGGG AACGGAAAAG GAGAATTCAA GTGTGACCCT
    CATGAGGCAA CGTGTTATGA TGATGGGAAG ACATACCACG TAGGAGAACA GTGGCAGAAG
    GAATATCTCG GTGCCATTTG CTCCTGCACA TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT
    GACAACTGCC GCAGACCTGG GGGTGAACCC AGTCCCGAAG GCACTACTGG CCAGTCCTAC
    AACCAGTATT CTCAGAGATA CCATCAGAGA ACAAACACTA ATGTTAATTG CCCAATTGAG
    TGCTTCATGC CTTTAGATGT ACAGGCTGAC AGAGAAGATT CCCGAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001352450.1
    CDS267..7337
    Translation

    Target ORF information:

    RefSeq Version NM_001365521.1
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant 16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001365521.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC
    GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG
    GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA
    TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG
    GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG
    ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA
    ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC
    AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG
    CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG
    CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT
    ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT
    CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGACG AGCTTCCCCA ACTGGTAACC
    CTTCCACACC CCAATCTTCA TGGACCAGAG ATCTTGGATG TTCCTTCCAC AGTTCAAAAG
    ACCCCTTTCG TCACCCACCC TGGGTATGAC ACTGGAAATG GTATTCAGCT TCCTGGCACT
    TCTGGTCAGC AACCCAGTGT TGGGCAACAA ATGATCTTTG AGGAACATGG TTTTAGGCGG
    ACCACACCGC CCACAACGGC CACCCCCATA AGGCATAGGC CAAGACCATA CCCGCCGAAT
    GTAGGACAAG AAGCTCTCTC TCAGACAACC ATCTCATGGG CCCCATTCCA GGACACTTCT
    GAGTACATCA TTTCATGTCA TCCTGTTGGC ACTGATGAAG AACCCTTACA GTTCAGGGTT
    CCTGGAACTT CTACCAGTGC CACTCTGACA GGCCTCACCA GAGGTGCCAC CTACAACGTC
    ATAGTGGAGG CACTGAAAGA CCAGCAGAGG CATAAGGTTC GGGAAGAGGT TGTTACCGTG
    GGCAACTCTG TCAACGAAGG CTTGAACCAA CCTACGGATG ACTCGTGCTT TGACCCCTAC
    ACAGTTTCCC ATTATGCCGT TGGAGATGAG TGGGAACGAA TGTCTGAATC AGGCTTTAAA
    CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT GGTCATTTCA GATGTGATTC ATCTAGATGG
    TGCCATGACA ATGGTGTGAA CTACAAGATT GGAGAGAAGT GGGACCGTCA GGGAGAAAAT
    GGCCAGATGA TGAGCTGCAC ATGTCTTGGG AACGGAAAAG GAGAATTCAA GTGTGACCCT
    CATGAGGCAA CGTGTTATGA TGATGGGAAG ACATACCACG TAGGAGAACA GTGGCAGAAG
    GAATATCTCG GTGCCATTTG CTCCTGCACA TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT
    GACAACTGCC GCAGACCTGG GGGTGAACCC AGTCCCGAAG GCACTACTGG CCAGTCCTAC
    AACCAGTATT CTCAGAGATA CCATCAGAGA ACAAACACTA ATGTTAATTG CCCAATTGAG
    TGCTTCATGC CTTTAGATGT ACAGGCTGAC AGAGAAGATT CCCGAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu89217
    Clone ID Related Accession (Same CDS sequence) NM_001365522.1 , XM_017003694.1
    Accession Version NM_001365522.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-29
    Organism Homo sapiens(human)
    Product fibronectin isoform 17 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012462.5 and AC073284.5. On Aug 30, 2018 this sequence version replaced XM_017003694.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.14796.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC
    GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG
    GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA
    TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG
    GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG
    ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA
    ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC
    AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG
    CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG
    CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT
    ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT
    CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGTTC AAAAGACCCC TTTCGTCACC
    CACCCTGGGT ATGACACTGG AAATGGTATT CAGCTTCCTG GCACTTCTGG TCAGCAACCC
    AGTGTTGGGC AACAAATGAT CTTTGAGGAA CATGGTTTTA GGCGGACCAC ACCGCCCACA
    ACGGCCACCC CCATAAGGCA TAGGCCAAGA CCATACCCGC CGAATGTAGG ACAAGAAGCT
    CTCTCTCAGA CAACCATCTC ATGGGCCCCA TTCCAGGACA CTTCTGAGTA CATCATTTCA
    TGTCATCCTG TTGGCACTGA TGAAGAACCC TTACAGTTCA GGGTTCCTGG AACTTCTACC
    AGTGCCACTC TGACAGGCCT CACCAGAGGT GCCACCTACA ACGTCATAGT GGAGGCACTG
    AAAGACCAGC AGAGGCATAA GGTTCGGGAA GAGGTTGTTA CCGTGGGCAA CTCTGTCAAC
    GAAGGCTTGA ACCAACCTAC GGATGACTCG TGCTTTGACC CCTACACAGT TTCCCATTAT
    GCCGTTGGAG ATGAGTGGGA ACGAATGTCT GAATCAGGCT TTAAACTGTT GTGCCAGTGC
    TTAGGCTTTG GAAGTGGTCA TTTCAGATGT GATTCATCTA GATGGTGCCA TGACAATGGT
    GTGAACTACA AGATTGGAGA GAAGTGGGAC CGTCAGGGAG AAAATGGCCA GATGATGAGC
    TGCACATGTC TTGGGAACGG AAAAGGAGAA TTCAAGTGTG ACCCTCATGA GGCAACGTGT
    TATGATGATG GGAAGACATA CCACGTAGGA GAACAGTGGC AGAAGGAATA TCTCGGTGCC
    ATTTGCTCCT GCACATGCTT TGGAGGCCAG CGGGGCTGGC GCTGTGACAA CTGCCGCAGA
    CCTGGGGGTG AACCCAGTCC CGAAGGCACT ACTGGCCAGT CCTACAACCA GTATTCTCAG
    AGATACCATC AGAGAACAAA CACTAATGTT AATTGCCCAA TTGAGTGCTT CATGCCTTTA
    GATGTACAGG CTGACAGAGA AGATTCCCGA GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001352451.1
    CDS267..7262
    Translation

    Target ORF information:

    RefSeq Version NM_001365522.1
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant 17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001365522.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC
    GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG
    GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA
    TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG
    GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG
    ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA
    ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC
    AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG
    CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG
    CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT
    ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT
    CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGTTC AAAAGACCCC TTTCGTCACC
    CACCCTGGGT ATGACACTGG AAATGGTATT CAGCTTCCTG GCACTTCTGG TCAGCAACCC
    AGTGTTGGGC AACAAATGAT CTTTGAGGAA CATGGTTTTA GGCGGACCAC ACCGCCCACA
    ACGGCCACCC CCATAAGGCA TAGGCCAAGA CCATACCCGC CGAATGTAGG ACAAGAAGCT
    CTCTCTCAGA CAACCATCTC ATGGGCCCCA TTCCAGGACA CTTCTGAGTA CATCATTTCA
    TGTCATCCTG TTGGCACTGA TGAAGAACCC TTACAGTTCA GGGTTCCTGG AACTTCTACC
    AGTGCCACTC TGACAGGCCT CACCAGAGGT GCCACCTACA ACGTCATAGT GGAGGCACTG
    AAAGACCAGC AGAGGCATAA GGTTCGGGAA GAGGTTGTTA CCGTGGGCAA CTCTGTCAAC
    GAAGGCTTGA ACCAACCTAC GGATGACTCG TGCTTTGACC CCTACACAGT TTCCCATTAT
    GCCGTTGGAG ATGAGTGGGA ACGAATGTCT GAATCAGGCT TTAAACTGTT GTGCCAGTGC
    TTAGGCTTTG GAAGTGGTCA TTTCAGATGT GATTCATCTA GATGGTGCCA TGACAATGGT
    GTGAACTACA AGATTGGAGA GAAGTGGGAC CGTCAGGGAG AAAATGGCCA GATGATGAGC
    TGCACATGTC TTGGGAACGG AAAAGGAGAA TTCAAGTGTG ACCCTCATGA GGCAACGTGT
    TATGATGATG GGAAGACATA CCACGTAGGA GAACAGTGGC AGAAGGAATA TCTCGGTGCC
    ATTTGCTCCT GCACATGCTT TGGAGGCCAG CGGGGCTGGC GCTGTGACAA CTGCCGCAGA
    CCTGGGGGTG AACCCAGTCC CGAAGGCACT ACTGGCCAGT CCTACAACCA GTATTCTCAG
    AGATACCATC AGAGAACAAA CACTAATGTT AATTGCCCAA TTGAGTGCTT CATGCCTTTA
    GATGTACAGG CTGACAGAGA AGATTCCCGA GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu104833
    Clone ID Related Accession (Same CDS sequence) XM_024452769.1
    Accession Version XM_024452769.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product fibronectin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA
    ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT
    GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG
    CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG
    GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG
    TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC
    ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC
    CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC
    ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT
    CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC
    ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT
    GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG
    ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT
    ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA
    AAGACAGTTC AAAAGACCCC TTTCGTCACC CACCCTGGGT ATGACACTGG AAATGGTATT
    CAGCTTCCTG GCACTTCTGG TCAGCAACCC AGTGTTGGGC AACAAATGAT CTTTGAGGAA
    CATGGTTTTA GGCGGACCAC ACCGCCCACA ACGGCCACCC CCATAAGGCA TAGGCCAAGA
    CCATACCCGC CGAATGTAGG TGAGGAAATC CAAATTGGTC ACATCCCCAG GGAAGATGTA
    GACTATCACC TGTACCCACA CGGTCCGGGA CTCAATCCAA ATGCCTCTAC AGGACAAGAA
    GCTCTCTCTC AGACAACCAT CTCATGGGCC CCATTCCAGG ACACTTCTGA GTACATCATT
    TCATGTCATC CTGTTGGCAC TGATGAAGAA CCCTTACAGT TCAGGGTTCC TGGAACTTCT
    ACCAGTGCCA CTCTGACAGG CCTCACCAGA GGTGCCACCT ACAACGTCAT AGTGGAGGCA
    CTGAAAGACC AGCAGAGGCA TAAGGTTCGG GAAGAGGTTG TTACCGTGGG CAACTCTGTC
    AACGAAGGCT TGAACCAACC TACGGATGAC TCGTGCTTTG ACCCCTACAC AGTTTCCCAT
    TATGCCGTTG GAGATGAGTG GGAACGAATG TCTGAATCAG GCTTTAAACT GTTGTGCCAG
    TGCTTAGGCT TTGGAAGTGG TCATTTCAGA TGTGATTCAT CTAGATGGTG CCATGACAAT
    GGTGTGAACT ACAAGATTGG AGAGAAGTGG GACCGTCAGG GAGAAAATGG CCAGATGATG
    AGCTGCACAT GTCTTGGGAA CGGAAAAGGA GAATTCAAGT GTGACCCTCA TGAGGCAACG
    TGTTATGATG ATGGGAAGAC ATACCACGTA GGAGAACAGT GGCAGAAGGA ATATCTCGGT
    GCCATTTGCT CCTGCACATG CTTTGGAGGC CAGCGGGGCT GGCGCTGTGA CAACTGCCGC
    AGACCTGGGG GTGAACCCAG TCCCGAAGGC ACTACTGGCC AGTCCTACAA CCAGTATTCT
    CAGAGATACC ATCAGAGAAC AAACACTAAT GTTAATTGCC CAATTGAGTG CTTCATGCCT
    TTAGATGTAC AGGCTGACAG AGAAGATTCC CGAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024308537.1
    CDS366..7724
    Translation

    Target ORF information:

    RefSeq Version XM_024452769.1
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024452769.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA
    ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT
    GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG
    CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG
    GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG
    TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC
    ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC
    CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC
    ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT
    CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC
    ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT
    GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG
    ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT
    ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA
    AAGACAGTTC AAAAGACCCC TTTCGTCACC CACCCTGGGT ATGACACTGG AAATGGTATT
    CAGCTTCCTG GCACTTCTGG TCAGCAACCC AGTGTTGGGC AACAAATGAT CTTTGAGGAA
    CATGGTTTTA GGCGGACCAC ACCGCCCACA ACGGCCACCC CCATAAGGCA TAGGCCAAGA
    CCATACCCGC CGAATGTAGG TGAGGAAATC CAAATTGGTC ACATCCCCAG GGAAGATGTA
    GACTATCACC TGTACCCACA CGGTCCGGGA CTCAATCCAA ATGCCTCTAC AGGACAAGAA
    GCTCTCTCTC AGACAACCAT CTCATGGGCC CCATTCCAGG ACACTTCTGA GTACATCATT
    TCATGTCATC CTGTTGGCAC TGATGAAGAA CCCTTACAGT TCAGGGTTCC TGGAACTTCT
    ACCAGTGCCA CTCTGACAGG CCTCACCAGA GGTGCCACCT ACAACGTCAT AGTGGAGGCA
    CTGAAAGACC AGCAGAGGCA TAAGGTTCGG GAAGAGGTTG TTACCGTGGG CAACTCTGTC
    AACGAAGGCT TGAACCAACC TACGGATGAC TCGTGCTTTG ACCCCTACAC AGTTTCCCAT
    TATGCCGTTG GAGATGAGTG GGAACGAATG TCTGAATCAG GCTTTAAACT GTTGTGCCAG
    TGCTTAGGCT TTGGAAGTGG TCATTTCAGA TGTGATTCAT CTAGATGGTG CCATGACAAT
    GGTGTGAACT ACAAGATTGG AGAGAAGTGG GACCGTCAGG GAGAAAATGG CCAGATGATG
    AGCTGCACAT GTCTTGGGAA CGGAAAAGGA GAATTCAAGT GTGACCCTCA TGAGGCAACG
    TGTTATGATG ATGGGAAGAC ATACCACGTA GGAGAACAGT GGCAGAAGGA ATATCTCGGT
    GCCATTTGCT CCTGCACATG CTTTGGAGGC CAGCGGGGCT GGCGCTGTGA CAACTGCCGC
    AGACCTGGGG GTGAACCCAG TCCCGAAGGC ACTACTGGCC AGTCCTACAA CCAGTATTCT
    CAGAGATACC ATCAGAGAAC AAACACTAAT GTTAATTGCC CAATTGAGTG CTTCATGCCT
    TTAGATGTAC AGGCTGACAG AGAAGATTCC CGAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu104834
    Clone ID Related Accession (Same CDS sequence) XM_024452770.1
    Accession Version XM_024452770.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7107bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product fibronectin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC
    GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG
    GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA
    TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG
    GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG
    ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA
    ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC
    AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG
    CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG
    CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT
    ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT
    CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGTTC AAAAGACCCC TTTCGTCACC
    CACCCTGGGT ATGACACTGG AAATGGTATT CAGCTTCCTG GCACTTCTGG TCAGCAACCC
    AGTGTTGGGC AACAAATGAT CTTTGAGGAA CATGGTTTTA GGCGGACCAC ACCGCCCACA
    ACGGCCACCC CCATAAGGCA TAGGCCAAGA CCATACCCGC CGAATGTAGG TGAGGAAATC
    CAAATTGGTC ACATCCCCAG GGAAGATGTA GACTATCACC TGTACCCACA CGGTCCGGGA
    CTCAATCCAA ATGCCTCTAC AGGACAAGAA GCTCTCTCTC AGACAACCAT CTCATGGGCC
    CCATTCCAGG ACACTTCTGA GTACATCATT TCATGTCATC CTGTTGGCAC TGATGAAGAA
    CCCTTACAGT TCAGGGTTCC TGGAACTTCT ACCAGTGCCA CTCTGACAGG CCTCACCAGA
    GGTGCCACCT ACAACGTCAT AGTGGAGGCA CTGAAAGACC AGCAGAGGCA TAAGGTTCGG
    GAAGAGGTTG TTACCGTGGG CAACTCTGTC AACGAAGGCT TGAACCAACC TACGGATGAC
    TCGTGCTTTG ACCCCTACAC AGTTTCCCAT TATGCCGTTG GAGATGAGTG GGAACGAATG
    TCTGAATCAG GCTTTAAACT GTTGTGCCAG TGCTTAGGCT TTGGAAGTGG TCATTTCAGA
    TGTGATTCAT CTAGATGGTG CCATGACAAT GGTGTGAACT ACAAGATTGG AGAGAAGTGG
    GACCGTCAGG GAGAAAATGG CCAGATGATG AGCTGCACAT GTCTTGGGAA CGGAAAAGGA
    GAATTCAAGT GTGACCCTCA TGAGGCAACG TGTTATGATG ATGGGAAGAC ATACCACGTA
    GGAGAACAGT GGCAGAAGGA ATATCTCGGT GCCATTTGCT CCTGCACATG CTTTGGAGGC
    CAGCGGGGCT GGCGCTGTGA CAACTGCCGC AGACCTGGGG GTGAACCCAG TCCCGAAGGC
    ACTACTGGCC AGTCCTACAA CCAGTATTCT CAGAGATACC ATCAGAGAAC AAACACTGTA
    AGTGCATTAG CAGCACAAGT GTGTTCCCTC ATACTAGACA GTCTCTTTCT ACAGGTATCT
    TTCTTCAGAA TGAACCAAGT GTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024308538.1
    CDS366..7472
    Translation

    Target ORF information:

    RefSeq Version XM_024452770.1
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024452770.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC
    GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG
    GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA
    TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG
    GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG
    ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA
    ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC
    AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG
    CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG
    CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT
    ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT
    CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGTTC AAAAGACCCC TTTCGTCACC
    CACCCTGGGT ATGACACTGG AAATGGTATT CAGCTTCCTG GCACTTCTGG TCAGCAACCC
    AGTGTTGGGC AACAAATGAT CTTTGAGGAA CATGGTTTTA GGCGGACCAC ACCGCCCACA
    ACGGCCACCC CCATAAGGCA TAGGCCAAGA CCATACCCGC CGAATGTAGG TGAGGAAATC
    CAAATTGGTC ACATCCCCAG GGAAGATGTA GACTATCACC TGTACCCACA CGGTCCGGGA
    CTCAATCCAA ATGCCTCTAC AGGACAAGAA GCTCTCTCTC AGACAACCAT CTCATGGGCC
    CCATTCCAGG ACACTTCTGA GTACATCATT TCATGTCATC CTGTTGGCAC TGATGAAGAA
    CCCTTACAGT TCAGGGTTCC TGGAACTTCT ACCAGTGCCA CTCTGACAGG CCTCACCAGA
    GGTGCCACCT ACAACGTCAT AGTGGAGGCA CTGAAAGACC AGCAGAGGCA TAAGGTTCGG
    GAAGAGGTTG TTACCGTGGG CAACTCTGTC AACGAAGGCT TGAACCAACC TACGGATGAC
    TCGTGCTTTG ACCCCTACAC AGTTTCCCAT TATGCCGTTG GAGATGAGTG GGAACGAATG
    TCTGAATCAG GCTTTAAACT GTTGTGCCAG TGCTTAGGCT TTGGAAGTGG TCATTTCAGA
    TGTGATTCAT CTAGATGGTG CCATGACAAT GGTGTGAACT ACAAGATTGG AGAGAAGTGG
    GACCGTCAGG GAGAAAATGG CCAGATGATG AGCTGCACAT GTCTTGGGAA CGGAAAAGGA
    GAATTCAAGT GTGACCCTCA TGAGGCAACG TGTTATGATG ATGGGAAGAC ATACCACGTA
    GGAGAACAGT GGCAGAAGGA ATATCTCGGT GCCATTTGCT CCTGCACATG CTTTGGAGGC
    CAGCGGGGCT GGCGCTGTGA CAACTGCCGC AGACCTGGGG GTGAACCCAG TCCCGAAGGC
    ACTACTGGCC AGTCCTACAA CCAGTATTCT CAGAGATACC ATCAGAGAAC AAACACTGTA
    AGTGCATTAG CAGCACAAGT GTGTTCCCTC ATACTAGACA GTCTCTTTCT ACAGGTATCT
    TTCTTCAGAA TGAACCAAGT GTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu89215
    Clone ID Related Accession (Same CDS sequence) XM_017003692.1
    Accession Version XM_017003692.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product fibronectin isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA
    ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT
    GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG
    CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG
    GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG
    TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC
    ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC
    CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC
    ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT
    CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC
    ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT
    GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG
    ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT
    ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA
    AAGACAGGAC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGGCCCCATT CCAGGACACT
    TCTGAGTACA TCATTTCATG TCATCCTGTT GGCACTGATG AAGAACCCTT ACAGTTCAGG
    GTTCCTGGAA CTTCTACCAG TGCCACTCTG ACAGGCCTCA CCAGAGGTGC CACCTACAAC
    GTCATAGTGG AGGCACTGAA AGACCAGCAG AGGCATAAGG TTCGGGAAGA GGTTGTTACC
    GTGGGCAACT CTGTCAACGA AGGCTTGAAC CAACCTACGG ATGACTCGTG CTTTGACCCC
    TACACAGTTT CCCATTATGC CGTTGGAGAT GAGTGGGAAC GAATGTCTGA ATCAGGCTTT
    AAACTGTTGT GCCAGTGCTT AGGCTTTGGA AGTGGTCATT TCAGATGTGA TTCATCTAGA
    TGGTGCCATG ACAATGGTGT GAACTACAAG ATTGGAGAGA AGTGGGACCG TCAGGGAGAA
    AATGGCCAGA TGATGAGCTG CACATGTCTT GGGAACGGAA AAGGAGAATT CAAGTGTGAC
    CCTCATGAGG CAACGTGTTA TGATGATGGG AAGACATACC ACGTAGGAGA ACAGTGGCAG
    AAGGAATATC TCGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC
    TGTGACAACT GCCGCAGACC TGGGGGTGAA CCCAGTCCCG AAGGCACTAC TGGCCAGTCC
    TACAACCAGT ATTCTCAGAG ATACCATCAG AGAACAAACA CTAATGTTAA TTGCCCAATT
    GAGTGCTTCA TGCCTTTAGA TGTACAGGCT GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016859181.1
    CDS366..7439
    Translation

    Target ORF information:

    RefSeq Version XM_017003692.1
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017003692.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA
    ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT
    GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG
    CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG
    GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG
    TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC
    ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC
    CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC
    ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT
    CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC
    ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT
    GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG
    ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT
    ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA
    AAGACAGGAC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGGCCCCATT CCAGGACACT
    TCTGAGTACA TCATTTCATG TCATCCTGTT GGCACTGATG AAGAACCCTT ACAGTTCAGG
    GTTCCTGGAA CTTCTACCAG TGCCACTCTG ACAGGCCTCA CCAGAGGTGC CACCTACAAC
    GTCATAGTGG AGGCACTGAA AGACCAGCAG AGGCATAAGG TTCGGGAAGA GGTTGTTACC
    GTGGGCAACT CTGTCAACGA AGGCTTGAAC CAACCTACGG ATGACTCGTG CTTTGACCCC
    TACACAGTTT CCCATTATGC CGTTGGAGAT GAGTGGGAAC GAATGTCTGA ATCAGGCTTT
    AAACTGTTGT GCCAGTGCTT AGGCTTTGGA AGTGGTCATT TCAGATGTGA TTCATCTAGA
    TGGTGCCATG ACAATGGTGT GAACTACAAG ATTGGAGAGA AGTGGGACCG TCAGGGAGAA
    AATGGCCAGA TGATGAGCTG CACATGTCTT GGGAACGGAA AAGGAGAATT CAAGTGTGAC
    CCTCATGAGG CAACGTGTTA TGATGATGGG AAGACATACC ACGTAGGAGA ACAGTGGCAG
    AAGGAATATC TCGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC
    TGTGACAACT GCCGCAGACC TGGGGGTGAA CCCAGTCCCG AAGGCACTAC TGGCCAGTCC
    TACAACCAGT ATTCTCAGAG ATACCATCAG AGAACAAACA CTAATGTTAA TTGCCCAATT
    GAGTGCTTCA TGCCTTTAGA TGTACAGGCT GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu89216
    Clone ID Related Accession (Same CDS sequence) XM_017003693.1 , NM_001365521.1
    Accession Version XM_017003693.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7071bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product fibronectin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC
    GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG
    GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA
    TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG
    GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG
    ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA
    ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC
    AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG
    CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG
    CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT
    ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT
    CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGACG AGCTTCCCCA ACTGGTAACC
    CTTCCACACC CCAATCTTCA TGGACCAGAG ATCTTGGATG TTCCTTCCAC AGTTCAAAAG
    ACCCCTTTCG TCACCCACCC TGGGTATGAC ACTGGAAATG GTATTCAGCT TCCTGGCACT
    TCTGGTCAGC AACCCAGTGT TGGGCAACAA ATGATCTTTG AGGAACATGG TTTTAGGCGG
    ACCACACCGC CCACAACGGC CACCCCCATA AGGCATAGGC CAAGACCATA CCCGCCGAAT
    GTAGGACAAG AAGCTCTCTC TCAGACAACC ATCTCATGGG CCCCATTCCA GGACACTTCT
    GAGTACATCA TTTCATGTCA TCCTGTTGGC ACTGATGAAG AACCCTTACA GTTCAGGGTT
    CCTGGAACTT CTACCAGTGC CACTCTGACA GGCCTCACCA GAGGTGCCAC CTACAACGTC
    ATAGTGGAGG CACTGAAAGA CCAGCAGAGG CATAAGGTTC GGGAAGAGGT TGTTACCGTG
    GGCAACTCTG TCAACGAAGG CTTGAACCAA CCTACGGATG ACTCGTGCTT TGACCCCTAC
    ACAGTTTCCC ATTATGCCGT TGGAGATGAG TGGGAACGAA TGTCTGAATC AGGCTTTAAA
    CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT GGTCATTTCA GATGTGATTC ATCTAGATGG
    TGCCATGACA ATGGTGTGAA CTACAAGATT GGAGAGAAGT GGGACCGTCA GGGAGAAAAT
    GGCCAGATGA TGAGCTGCAC ATGTCTTGGG AACGGAAAAG GAGAATTCAA GTGTGACCCT
    CATGAGGCAA CGTGTTATGA TGATGGGAAG ACATACCACG TAGGAGAACA GTGGCAGAAG
    GAATATCTCG GTGCCATTTG CTCCTGCACA TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT
    GACAACTGCC GCAGACCTGG GGGTGAACCC AGTCCCGAAG GCACTACTGG CCAGTCCTAC
    AACCAGTATT CTCAGAGATA CCATCAGAGA ACAAACACTA ATGTTAATTG CCCAATTGAG
    TGCTTCATGC CTTTAGATGT ACAGGCTGAC AGAGAAGATT CCCGAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016859182.1
    CDS366..7436
    Translation

    Target ORF information:

    RefSeq Version XM_017003693.1
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017003693.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC
    GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG
    GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA
    TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG
    GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG
    ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA
    ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC
    AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG
    CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG
    CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT
    ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT
    CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGACG AGCTTCCCCA ACTGGTAACC
    CTTCCACACC CCAATCTTCA TGGACCAGAG ATCTTGGATG TTCCTTCCAC AGTTCAAAAG
    ACCCCTTTCG TCACCCACCC TGGGTATGAC ACTGGAAATG GTATTCAGCT TCCTGGCACT
    TCTGGTCAGC AACCCAGTGT TGGGCAACAA ATGATCTTTG AGGAACATGG TTTTAGGCGG
    ACCACACCGC CCACAACGGC CACCCCCATA AGGCATAGGC CAAGACCATA CCCGCCGAAT
    GTAGGACAAG AAGCTCTCTC TCAGACAACC ATCTCATGGG CCCCATTCCA GGACACTTCT
    GAGTACATCA TTTCATGTCA TCCTGTTGGC ACTGATGAAG AACCCTTACA GTTCAGGGTT
    CCTGGAACTT CTACCAGTGC CACTCTGACA GGCCTCACCA GAGGTGCCAC CTACAACGTC
    ATAGTGGAGG CACTGAAAGA CCAGCAGAGG CATAAGGTTC GGGAAGAGGT TGTTACCGTG
    GGCAACTCTG TCAACGAAGG CTTGAACCAA CCTACGGATG ACTCGTGCTT TGACCCCTAC
    ACAGTTTCCC ATTATGCCGT TGGAGATGAG TGGGAACGAA TGTCTGAATC AGGCTTTAAA
    CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT GGTCATTTCA GATGTGATTC ATCTAGATGG
    TGCCATGACA ATGGTGTGAA CTACAAGATT GGAGAGAAGT GGGACCGTCA GGGAGAAAAT
    GGCCAGATGA TGAGCTGCAC ATGTCTTGGG AACGGAAAAG GAGAATTCAA GTGTGACCCT
    CATGAGGCAA CGTGTTATGA TGATGGGAAG ACATACCACG TAGGAGAACA GTGGCAGAAG
    GAATATCTCG GTGCCATTTG CTCCTGCACA TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT
    GACAACTGCC GCAGACCTGG GGGTGAACCC AGTCCCGAAG GCACTACTGG CCAGTCCTAC
    AACCAGTATT CTCAGAGATA CCATCAGAGA ACAAACACTA ATGTTAATTG CCCAATTGAG
    TGCTTCATGC CTTTAGATGT ACAGGCTGAC AGAGAAGATT CCCGAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu89217
    Clone ID Related Accession (Same CDS sequence) NM_001365522.1 , XM_017003694.1
    Accession Version XM_017003694.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product fibronectin isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC
    GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG
    GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA
    TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG
    GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG
    ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA
    ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC
    AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG
    CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG
    CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT
    ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT
    CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGTTC AAAAGACCCC TTTCGTCACC
    CACCCTGGGT ATGACACTGG AAATGGTATT CAGCTTCCTG GCACTTCTGG TCAGCAACCC
    AGTGTTGGGC AACAAATGAT CTTTGAGGAA CATGGTTTTA GGCGGACCAC ACCGCCCACA
    ACGGCCACCC CCATAAGGCA TAGGCCAAGA CCATACCCGC CGAATGTAGG ACAAGAAGCT
    CTCTCTCAGA CAACCATCTC ATGGGCCCCA TTCCAGGACA CTTCTGAGTA CATCATTTCA
    TGTCATCCTG TTGGCACTGA TGAAGAACCC TTACAGTTCA GGGTTCCTGG AACTTCTACC
    AGTGCCACTC TGACAGGCCT CACCAGAGGT GCCACCTACA ACGTCATAGT GGAGGCACTG
    AAAGACCAGC AGAGGCATAA GGTTCGGGAA GAGGTTGTTA CCGTGGGCAA CTCTGTCAAC
    GAAGGCTTGA ACCAACCTAC GGATGACTCG TGCTTTGACC CCTACACAGT TTCCCATTAT
    GCCGTTGGAG ATGAGTGGGA ACGAATGTCT GAATCAGGCT TTAAACTGTT GTGCCAGTGC
    TTAGGCTTTG GAAGTGGTCA TTTCAGATGT GATTCATCTA GATGGTGCCA TGACAATGGT
    GTGAACTACA AGATTGGAGA GAAGTGGGAC CGTCAGGGAG AAAATGGCCA GATGATGAGC
    TGCACATGTC TTGGGAACGG AAAAGGAGAA TTCAAGTGTG ACCCTCATGA GGCAACGTGT
    TATGATGATG GGAAGACATA CCACGTAGGA GAACAGTGGC AGAAGGAATA TCTCGGTGCC
    ATTTGCTCCT GCACATGCTT TGGAGGCCAG CGGGGCTGGC GCTGTGACAA CTGCCGCAGA
    CCTGGGGGTG AACCCAGTCC CGAAGGCACT ACTGGCCAGT CCTACAACCA GTATTCTCAG
    AGATACCATC AGAGAACAAA CACTAATGTT AATTGCCCAA TTGAGTGCTT CATGCCTTTA
    GATGTACAGG CTGACAGAGA AGATTCCCGA GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016859183.1
    CDS366..7361
    Translation

    Target ORF information:

    RefSeq Version XM_017003694.1
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017003694.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC
    GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG
    GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA
    TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG
    GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG
    ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA
    ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC
    AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG
    CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG
    CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT
    ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT
    CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGTTC AAAAGACCCC TTTCGTCACC
    CACCCTGGGT ATGACACTGG AAATGGTATT CAGCTTCCTG GCACTTCTGG TCAGCAACCC
    AGTGTTGGGC AACAAATGAT CTTTGAGGAA CATGGTTTTA GGCGGACCAC ACCGCCCACA
    ACGGCCACCC CCATAAGGCA TAGGCCAAGA CCATACCCGC CGAATGTAGG ACAAGAAGCT
    CTCTCTCAGA CAACCATCTC ATGGGCCCCA TTCCAGGACA CTTCTGAGTA CATCATTTCA
    TGTCATCCTG TTGGCACTGA TGAAGAACCC TTACAGTTCA GGGTTCCTGG AACTTCTACC
    AGTGCCACTC TGACAGGCCT CACCAGAGGT GCCACCTACA ACGTCATAGT GGAGGCACTG
    AAAGACCAGC AGAGGCATAA GGTTCGGGAA GAGGTTGTTA CCGTGGGCAA CTCTGTCAAC
    GAAGGCTTGA ACCAACCTAC GGATGACTCG TGCTTTGACC CCTACACAGT TTCCCATTAT
    GCCGTTGGAG ATGAGTGGGA ACGAATGTCT GAATCAGGCT TTAAACTGTT GTGCCAGTGC
    TTAGGCTTTG GAAGTGGTCA TTTCAGATGT GATTCATCTA GATGGTGCCA TGACAATGGT
    GTGAACTACA AGATTGGAGA GAAGTGGGAC CGTCAGGGAG AAAATGGCCA GATGATGAGC
    TGCACATGTC TTGGGAACGG AAAAGGAGAA TTCAAGTGTG ACCCTCATGA GGCAACGTGT
    TATGATGATG GGAAGACATA CCACGTAGGA GAACAGTGGC AGAAGGAATA TCTCGGTGCC
    ATTTGCTCCT GCACATGCTT TGGAGGCCAG CGGGGCTGGC GCTGTGACAA CTGCCGCAGA
    CCTGGGGGTG AACCCAGTCC CGAAGGCACT ACTGGCCAGT CCTACAACCA GTATTCTCAG
    AGATACCATC AGAGAACAAA CACTAATGTT AATTGCCCAA TTGAGTGCTT CATGCCTTTA
    GATGTACAGG CTGACAGAGA AGATTCCCGA GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu89218
    Clone ID Related Accession (Same CDS sequence) XM_017003695.1
    Accession Version XM_017003695.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6909bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product fibronectin isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA
    GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG
    CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC
    AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT
    GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC
    CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT
    CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA
    CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA
    GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG
    ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC
    CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC
    ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG
    TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT
    GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA
    GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC
    AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT
    GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA
    GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG
    AGTCAGCCTC TGGTTCAGAC TGCAGTAACC ACTATTCCTG CACCAACTGA CCTGAAGTTC
    ACTCAGGTCA CACCCACAAG CCTGAGCGCC CAGTGGACAC CACCCAATGT TCAGCTCACT
    GGATATCGAG TGCGGGTGAC CCCCAAGGAG AAGACCGGAC CAATGAAAGA AATCAACCTT
    GCTCCTGACA GCTCATCCGT GGTTGTATCA GGACTTATGG TGGCCACCAA ATATGAAGTG
    AGTGTCTATG CTCTTAAGGA CACTTTGACA AGCAGACCAG CTCAGGGAGT TGTCACCACT
    CTGGAGAATG TCAGCCCACC AAGAAGGGCT CGTGTGACAG ATGCTACTGA GACCACCATC
    ACCATTAGCT GGAGAACCAA GACTGAGACG ATCACTGGCT TCCAAGTTGA TGCCGTTCCA
    GCCAATGGCC AGACTCCAAT CCAGAGAACC ATCAAGCCAG ATGTCAGAAG CTACACCATC
    ACAGGTTTAC AACCAGGCAC TGACTACAAG ATCTACCTGT ACACCTTGAA TGACAATGCT
    CGGAGCTCCC CTGTGGTCAT CGACGCCTCC ACTGCCATTG ATGCACCATC CAACCTGCGT
    TTCCTGGCCA CCACACCCAA TTCCTTGCTG GTATCATGGC AGCCGCCACG TGCCAGGATT
    ACCGGCTACA TCATCAAGTA TGAGAAGCCT GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACAGA GGCTACTATT ACTGGCCTGG AACCGGGAAC CGAATATACA
    ATTTATGTCA TTGCCCTGAA GAATAATCAG AAGAGCGAGC CCCTGATTGG AAGGAAAAAG
    ACAGACGAGC TTCCCCAACT GGTAACCCTT CCACACCCCA ATCTTCATGG ACCAGAGATC
    TTGGATGTTC CTTCCACAGT TCAAAAGACC CCTTTCGTCA CCCACCCTGG GTATGACACT
    GGAAATGGTA TTCAGCTTCC TGGCACTTCT GGTCAGCAAC CCAGTGTTGG GCAACAAATG
    ATCTTTGAGG AACATGGTTT TAGGCGGACC ACACCGCCCA CAACGGCCAC CCCCATAAGG
    CATAGGCCAA GACCATACCC GCCGAATGTA GGTGAGGAAA TCCAAATTGG TCACATCCCC
    AGGGAAGATG TAGACTATCA CCTGTACCCA CACGGTCCGG GACTCAATCC AAATGCCTCT
    ACAGGACAAG AAGCTCTCTC TCAGACAACC ATCTCATGGG CCCCATTCCA GGACACTTCT
    GAGTACATCA TTTCATGTCA TCCTGTTGGC ACTGATGAAG AACCCTTACA GTTCAGGGTT
    CCTGGAACTT CTACCAGTGC CACTCTGACA GGCCTCACCA GAGGTGCCAC CTACAACGTC
    ATAGTGGAGG CACTGAAAGA CCAGCAGAGG CATAAGGTTC GGGAAGAGGT TGTTACCGTG
    GGCAACTCTG TCAACGAAGG CTTGAACCAA CCTACGGATG ACTCGTGCTT TGACCCCTAC
    ACAGTTTCCC ATTATGCCGT TGGAGATGAG TGGGAACGAA TGTCTGAATC AGGCTTTAAA
    CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT GGTCATTTCA GATGTGATTC ATCTAGATGG
    TGCCATGACA ATGGTGTGAA CTACAAGATT GGAGAGAAGT GGGACCGTCA GGGAGAAAAT
    GGCCAGATGA TGAGCTGCAC ATGTCTTGGG AACGGAAAAG GAGAATTCAA GTGTGACCCT
    CATGAGGCAA CGTGTTATGA TGATGGGAAG ACATACCACG TAGGAGAACA GTGGCAGAAG
    GAATATCTCG GTGCCATTTG CTCCTGCACA TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT
    GACAACTGCC GCAGACCTGG GGGTGAACCC AGTCCCGAAG GCACTACTGG CCAGTCCTAC
    AACCAGTATT CTCAGAGATA CCATCAGAGA ACAAACACTG TAAGTGCATT AGCAGCACAA
    GTGTGTTCCC TCATACTAGA CAGTCTCTTT CTACAGGTAT CTTTCTTCAG AATGAACCAA
    GTGTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016859184.1
    CDS366..7274
    Translation

    Target ORF information:

    RefSeq Version XM_017003695.1
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017003695.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA
    GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG
    CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC
    AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT
    GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC
    CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT
    CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA
    CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA
    GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG
    ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC
    CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC
    ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG
    TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT
    GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA
    GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC
    AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT
    GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA
    GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG
    AGTCAGCCTC TGGTTCAGAC TGCAGTAACC ACTATTCCTG CACCAACTGA CCTGAAGTTC
    ACTCAGGTCA CACCCACAAG CCTGAGCGCC CAGTGGACAC CACCCAATGT TCAGCTCACT
    GGATATCGAG TGCGGGTGAC CCCCAAGGAG AAGACCGGAC CAATGAAAGA AATCAACCTT
    GCTCCTGACA GCTCATCCGT GGTTGTATCA GGACTTATGG TGGCCACCAA ATATGAAGTG
    AGTGTCTATG CTCTTAAGGA CACTTTGACA AGCAGACCAG CTCAGGGAGT TGTCACCACT
    CTGGAGAATG TCAGCCCACC AAGAAGGGCT CGTGTGACAG ATGCTACTGA GACCACCATC
    ACCATTAGCT GGAGAACCAA GACTGAGACG ATCACTGGCT TCCAAGTTGA TGCCGTTCCA
    GCCAATGGCC AGACTCCAAT CCAGAGAACC ATCAAGCCAG ATGTCAGAAG CTACACCATC
    ACAGGTTTAC AACCAGGCAC TGACTACAAG ATCTACCTGT ACACCTTGAA TGACAATGCT
    CGGAGCTCCC CTGTGGTCAT CGACGCCTCC ACTGCCATTG ATGCACCATC CAACCTGCGT
    TTCCTGGCCA CCACACCCAA TTCCTTGCTG GTATCATGGC AGCCGCCACG TGCCAGGATT
    ACCGGCTACA TCATCAAGTA TGAGAAGCCT GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACAGA GGCTACTATT ACTGGCCTGG AACCGGGAAC CGAATATACA
    ATTTATGTCA TTGCCCTGAA GAATAATCAG AAGAGCGAGC CCCTGATTGG AAGGAAAAAG
    ACAGACGAGC TTCCCCAACT GGTAACCCTT CCACACCCCA ATCTTCATGG ACCAGAGATC
    TTGGATGTTC CTTCCACAGT TCAAAAGACC CCTTTCGTCA CCCACCCTGG GTATGACACT
    GGAAATGGTA TTCAGCTTCC TGGCACTTCT GGTCAGCAAC CCAGTGTTGG GCAACAAATG
    ATCTTTGAGG AACATGGTTT TAGGCGGACC ACACCGCCCA CAACGGCCAC CCCCATAAGG
    CATAGGCCAA GACCATACCC GCCGAATGTA GGTGAGGAAA TCCAAATTGG TCACATCCCC
    AGGGAAGATG TAGACTATCA CCTGTACCCA CACGGTCCGG GACTCAATCC AAATGCCTCT
    ACAGGACAAG AAGCTCTCTC TCAGACAACC ATCTCATGGG CCCCATTCCA GGACACTTCT
    GAGTACATCA TTTCATGTCA TCCTGTTGGC ACTGATGAAG AACCCTTACA GTTCAGGGTT
    CCTGGAACTT CTACCAGTGC CACTCTGACA GGCCTCACCA GAGGTGCCAC CTACAACGTC
    ATAGTGGAGG CACTGAAAGA CCAGCAGAGG CATAAGGTTC GGGAAGAGGT TGTTACCGTG
    GGCAACTCTG TCAACGAAGG CTTGAACCAA CCTACGGATG ACTCGTGCTT TGACCCCTAC
    ACAGTTTCCC ATTATGCCGT TGGAGATGAG TGGGAACGAA TGTCTGAATC AGGCTTTAAA
    CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT GGTCATTTCA GATGTGATTC ATCTAGATGG
    TGCCATGACA ATGGTGTGAA CTACAAGATT GGAGAGAAGT GGGACCGTCA GGGAGAAAAT
    GGCCAGATGA TGAGCTGCAC ATGTCTTGGG AACGGAAAAG GAGAATTCAA GTGTGACCCT
    CATGAGGCAA CGTGTTATGA TGATGGGAAG ACATACCACG TAGGAGAACA GTGGCAGAAG
    GAATATCTCG GTGCCATTTG CTCCTGCACA TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT
    GACAACTGCC GCAGACCTGG GGGTGAACCC AGTCCCGAAG GCACTACTGG CCAGTCCTAC
    AACCAGTATT CTCAGAGATA CCATCAGAGA ACAAACACTG TAAGTGCATT AGCAGCACAA
    GTGTGTTCCC TCATACTAGA CAGTCTCTTT CTACAGGTAT CTTTCTTCAG AATGAACCAA
    GTGTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24915
    Clone ID Related Accession (Same CDS sequence) NM_212476.2
    Accession Version NM_212476.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-29
    Organism Homo sapiens(human)
    Product fibronectin isoform 5 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX640608.1, AJ535086.1, X02761.1, AI033037.1, U60068.1, BQ005645.1 and CR749317.1. On Jan 9, 2016 this sequence version replaced NM_212476.1. Transcript Variant: This variant (5) lacks two in-frame exons in the coding region, as compared to variant 1. Isoform 5 encoded by this variant thus lacks two internal segments, as compared to isoform 1. The encoded isoform (5) may undergo proteolytic processing similar to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX640608.1, DRR138524.403056.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA
    GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG
    CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC
    AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT
    GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC
    CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT
    CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA
    CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA
    GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG
    ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC
    CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC
    ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG
    TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT
    GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA
    GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC
    AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT
    GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA
    GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG
    AGTCAGCCTC TGGTTCAGAC TGCAGTAACC ACTATTCCTG CACCAACTGA CCTGAAGTTC
    ACTCAGGTCA CACCCACAAG CCTGAGCGCC CAGTGGACAC CACCCAATGT TCAGCTCACT
    GGATATCGAG TGCGGGTGAC CCCCAAGGAG AAGACCGGAC CAATGAAAGA AATCAACCTT
    GCTCCTGACA GCTCATCCGT GGTTGTATCA GGACTTATGG TGGCCACCAA ATATGAAGTG
    AGTGTCTATG CTCTTAAGGA CACTTTGACA AGCAGACCAG CTCAGGGAGT TGTCACCACT
    CTGGAGAATG TCAGCCCACC AAGAAGGGCT CGTGTGACAG ATGCTACTGA GACCACCATC
    ACCATTAGCT GGAGAACCAA GACTGAGACG ATCACTGGCT TCCAAGTTGA TGCCGTTCCA
    GCCAATGGCC AGACTCCAAT CCAGAGAACC ATCAAGCCAG ATGTCAGAAG CTACACCATC
    ACAGGTTTAC AACCAGGCAC TGACTACAAG ATCTACCTGT ACACCTTGAA TGACAATGCT
    CGGAGCTCCC CTGTGGTCAT CGACGCCTCC ACTGCCATTG ATGCACCATC CAACCTGCGT
    TTCCTGGCCA CCACACCCAA TTCCTTGCTG GTATCATGGC AGCCGCCACG TGCCAGGATT
    ACCGGCTACA TCATCAAGTA TGAGAAGCCT GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACAGA GGCTACTATT ACTGGCCTGG AACCGGGAAC CGAATATACA
    ATTTATGTCA TTGCCCTGAA GAATAATCAG AAGAGCGAGC CCCTGATTGG AAGGAAAAAG
    ACAGACGAGC TTCCCCAACT GGTAACCCTT CCACACCCCA ATCTTCATGG ACCAGAGATC
    TTGGATGTTC CTTCCACAGT TCAAAAGACC CCTTTCGTCA CCCACCCTGG GTATGACACT
    GGAAATGGTA TTCAGCTTCC TGGCACTTCT GGTCAGCAAC CCAGTGTTGG GCAACAAATG
    ATCTTTGAGG AACATGGTTT TAGGCGGACC ACACCGCCCA CAACGGCCAC CCCCATAAGG
    CATAGGCCAA GACCATACCC GCCGAATGTA GGTGAGGAAA TCCAAATTGG TCACATCCCC
    AGGGAAGATG TAGACTATCA CCTGTACCCA CACGGTCCGG GACTCAATCC AAATGCCTCT
    ACAGGACAAG AAGCTCTCTC TCAGACAACC ATCTCATGGG CCCCATTCCA GGACACTTCT
    GAGTACATCA TTTCATGTCA TCCTGTTGGC ACTGATGAAG AACCCTTACA GTTCAGGGTT
    CCTGGAACTT CTACCAGTGC CACTCTGACA GGCCTCACCA GAGGTGCCAC CTACAACATC
    ATAGTGGAGG CACTGAAAGA CCAGCAGAGG CATAAGGTTC GGGAAGAGGT TGTTACCGTG
    GGCAACTCTG TCAACGAAGG CTTGAACCAA CCTACGGATG ACTCGTGCTT TGACCCCTAC
    ACAGTTTCCC ATTATGCCGT TGGAGATGAG TGGGAACGAA TGTCTGAATC AGGCTTTAAA
    CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT GGTCATTTCA GATGTGATTC ATCTAGATGG
    TGCCATGACA ATGGTGTGAA CTACAAGATT GGAGAGAAGT GGGACCGTCA GGGAGAAAAT
    GGCCAGATGA TGAGCTGCAC ATGTCTTGGG AACGGAAAAG GAGAATTCAA GTGTGACCCT
    CATGAGGCAA CGTGTTATGA TGATGGGAAG ACATACCACG TAGGAGAACA GTGGCAGAAG
    GAATATCTCG GTGCCATTTG CTCCTGCACA TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT
    GACAACTGCC GCAGACCTGG GGGTGAACCC AGTCCCGAAG GCACTACTGG CCAGTCCTAC
    AACCAGTATT CTCAGAGATA CCATCAGAGA ACAAACACTA ATGTTAATTG CCCAATTGAG
    TGCTTCATGC CTTTAGATGT ACAGGCTGAC AGAGAAGATT CCCGAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_997641.1
    CDS267..7157
    Translation

    Target ORF information:

    RefSeq Version NM_212476.2
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_212476.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA
    GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG
    CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC
    AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT
    GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC
    CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT
    CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA
    CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA
    GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG
    ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC
    CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC
    ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG
    TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT
    GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA
    GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC
    AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT
    GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA
    GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG
    AGTCAGCCTC TGGTTCAGAC TGCAGTAACC ACTATTCCTG CACCAACTGA CCTGAAGTTC
    ACTCAGGTCA CACCCACAAG CCTGAGCGCC CAGTGGACAC CACCCAATGT TCAGCTCACT
    GGATATCGAG TGCGGGTGAC CCCCAAGGAG AAGACCGGAC CAATGAAAGA AATCAACCTT
    GCTCCTGACA GCTCATCCGT GGTTGTATCA GGACTTATGG TGGCCACCAA ATATGAAGTG
    AGTGTCTATG CTCTTAAGGA CACTTTGACA AGCAGACCAG CTCAGGGAGT TGTCACCACT
    CTGGAGAATG TCAGCCCACC AAGAAGGGCT CGTGTGACAG ATGCTACTGA GACCACCATC
    ACCATTAGCT GGAGAACCAA GACTGAGACG ATCACTGGCT TCCAAGTTGA TGCCGTTCCA
    GCCAATGGCC AGACTCCAAT CCAGAGAACC ATCAAGCCAG ATGTCAGAAG CTACACCATC
    ACAGGTTTAC AACCAGGCAC TGACTACAAG ATCTACCTGT ACACCTTGAA TGACAATGCT
    CGGAGCTCCC CTGTGGTCAT CGACGCCTCC ACTGCCATTG ATGCACCATC CAACCTGCGT
    TTCCTGGCCA CCACACCCAA TTCCTTGCTG GTATCATGGC AGCCGCCACG TGCCAGGATT
    ACCGGCTACA TCATCAAGTA TGAGAAGCCT GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACAGA GGCTACTATT ACTGGCCTGG AACCGGGAAC CGAATATACA
    ATTTATGTCA TTGCCCTGAA GAATAATCAG AAGAGCGAGC CCCTGATTGG AAGGAAAAAG
    ACAGACGAGC TTCCCCAACT GGTAACCCTT CCACACCCCA ATCTTCATGG ACCAGAGATC
    TTGGATGTTC CTTCCACAGT TCAAAAGACC CCTTTCGTCA CCCACCCTGG GTATGACACT
    GGAAATGGTA TTCAGCTTCC TGGCACTTCT GGTCAGCAAC CCAGTGTTGG GCAACAAATG
    ATCTTTGAGG AACATGGTTT TAGGCGGACC ACACCGCCCA CAACGGCCAC CCCCATAAGG
    CATAGGCCAA GACCATACCC GCCGAATGTA GGTGAGGAAA TCCAAATTGG TCACATCCCC
    AGGGAAGATG TAGACTATCA CCTGTACCCA CACGGTCCGG GACTCAATCC AAATGCCTCT
    ACAGGACAAG AAGCTCTCTC TCAGACAACC ATCTCATGGG CCCCATTCCA GGACACTTCT
    GAGTACATCA TTTCATGTCA TCCTGTTGGC ACTGATGAAG AACCCTTACA GTTCAGGGTT
    CCTGGAACTT CTACCAGTGC CACTCTGACA GGCCTCACCA GAGGTGCCAC CTACAACATC
    ATAGTGGAGG CACTGAAAGA CCAGCAGAGG CATAAGGTTC GGGAAGAGGT TGTTACCGTG
    GGCAACTCTG TCAACGAAGG CTTGAACCAA CCTACGGATG ACTCGTGCTT TGACCCCTAC
    ACAGTTTCCC ATTATGCCGT TGGAGATGAG TGGGAACGAA TGTCTGAATC AGGCTTTAAA
    CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT GGTCATTTCA GATGTGATTC ATCTAGATGG
    TGCCATGACA ATGGTGTGAA CTACAAGATT GGAGAGAAGT GGGACCGTCA GGGAGAAAAT
    GGCCAGATGA TGAGCTGCAC ATGTCTTGGG AACGGAAAAG GAGAATTCAA GTGTGACCCT
    CATGAGGCAA CGTGTTATGA TGATGGGAAG ACATACCACG TAGGAGAACA GTGGCAGAAG
    GAATATCTCG GTGCCATTTG CTCCTGCACA TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT
    GACAACTGCC GCAGACCTGG GGGTGAACCC AGTCCCGAAG GCACTACTGG CCAGTCCTAC
    AACCAGTATT CTCAGAGATA CCATCAGAGA ACAAACACTA ATGTTAATTG CCCAATTGAG
    TGCTTCATGC CTTTAGATGT ACAGGCTGAC AGAGAAGATT CCCGAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24997
    Clone ID Related Accession (Same CDS sequence) NM_212474.2
    Accession Version NM_212474.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Homo sapiens(human)
    Product fibronectin isoform 6 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL832202.1, AJ535086.1, X02761.1, AI033037.1, U60068.1, BQ005645.1 and CR749317.1. On Jan 9, 2016 this sequence version replaced NM_212474.1. Transcript Variant: This variant (6) lacks two in-frame exons and an in-frame segment in the coding region, as compared to variant 1. Isoform 6 encoded by this variant thus lacks three internal segments, as compared to isoform 1. The encoded isoform (6) may undergo proteolytic processing similar to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC078656.1, SRR1660803.174926.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA
    GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG
    CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC
    AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT
    GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC
    CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT
    CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA
    CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA
    GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG
    ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC
    CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC
    ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG
    TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT
    GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA
    GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC
    AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT
    GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA
    GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG
    AGTCAGCCTC TGGTTCAGAC TGCAGTAACC ACTATTCCTG CACCAACTGA CCTGAAGTTC
    ACTCAGGTCA CACCCACAAG CCTGAGCGCC CAGTGGACAC CACCCAATGT TCAGCTCACT
    GGATATCGAG TGCGGGTGAC CCCCAAGGAG AAGACCGGAC CAATGAAAGA AATCAACCTT
    GCTCCTGACA GCTCATCCGT GGTTGTATCA GGACTTATGG TGGCCACCAA ATATGAAGTG
    AGTGTCTATG CTCTTAAGGA CACTTTGACA AGCAGACCAG CTCAGGGAGT TGTCACCACT
    CTGGAGAATG TCAGCCCACC AAGAAGGGCT CGTGTGACAG ATGCTACTGA GACCACCATC
    ACCATTAGCT GGAGAACCAA GACTGAGACG ATCACTGGCT TCCAAGTTGA TGCCGTTCCA
    GCCAATGGCC AGACTCCAAT CCAGAGAACC ATCAAGCCAG ATGTCAGAAG CTACACCATC
    ACAGGTTTAC AACCAGGCAC TGACTACAAG ATCTACCTGT ACACCTTGAA TGACAATGCT
    CGGAGCTCCC CTGTGGTCAT CGACGCCTCC ACTGCCATTG ATGCACCATC CAACCTGCGT
    TTCCTGGCCA CCACACCCAA TTCCTTGCTG GTATCATGGC AGCCGCCACG TGCCAGGATT
    ACCGGCTACA TCATCAAGTA TGAGAAGCCT GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACAGA GGCTACTATT ACTGGCCTGG AACCGGGAAC CGAATATACA
    ATTTATGTCA TTGCCCTGAA GAATAATCAG AAGAGCGAGC CCCTGATTGG AAGGAAAAAG
    ACAGGACAAG AAGCTCTCTC TCAGACAACC ATCTCATGGG CCCCATTCCA GGACACTTCT
    GAGTACATCA TTTCATGTCA TCCTGTTGGC ACTGATGAAG AACCCTTACA GTTCAGGGTT
    CCTGGAACTT CTACCAGTGC CACTCTGACA GGCCTCACCA GAGGTGCCAC CTACAACATC
    ATAGTGGAGG CACTGAAAGA CCAGCAGAGG CATAAGGTTC GGGAAGAGGT TGTTACCGTG
    GGCAACTCTG TCAACGAAGG CTTGAACCAA CCTACGGATG ACTCGTGCTT TGACCCCTAC
    ACAGTTTCCC ATTATGCCGT TGGAGATGAG TGGGAACGAA TGTCTGAATC AGGCTTTAAA
    CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT GGTCATTTCA GATGTGATTC ATCTAGATGG
    TGCCATGACA ATGGTGTGAA CTACAAGATT GGAGAGAAGT GGGACCGTCA GGGAGAAAAT
    GGCCAGATGA TGAGCTGCAC ATGTCTTGGG AACGGAAAAG GAGAATTCAA GTGTGACCCT
    CATGAGGCAA CGTGTTATGA TGATGGGAAG ACATACCACG TAGGAGAACA GTGGCAGAAG
    GAATATCTCG GTGCCATTTG CTCCTGCACA TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT
    GACAACTGCC GCAGACCTGG GGGTGAACCC AGTCCCGAAG GCACTACTGG CCAGTCCTAC
    AACCAGTATT CTCAGAGATA CCATCAGAGA ACAAACACTA ATGTTAATTG CCCAATTGAG
    TGCTTCATGC CTTTAGATGT ACAGGCTGAC AGAGAAGATT CCCGAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_997639.1
    CDS267..6797
    Translation

    Target ORF information:

    RefSeq Version NM_212474.2
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_212474.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA
    GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG
    CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC
    AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT
    GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC
    CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT
    CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA
    CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA
    GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG
    ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC
    CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC
    ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG
    TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT
    GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA
    GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC
    AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT
    GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA
    GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG
    AGTCAGCCTC TGGTTCAGAC TGCAGTAACC ACTATTCCTG CACCAACTGA CCTGAAGTTC
    ACTCAGGTCA CACCCACAAG CCTGAGCGCC CAGTGGACAC CACCCAATGT TCAGCTCACT
    GGATATCGAG TGCGGGTGAC CCCCAAGGAG AAGACCGGAC CAATGAAAGA AATCAACCTT
    GCTCCTGACA GCTCATCCGT GGTTGTATCA GGACTTATGG TGGCCACCAA ATATGAAGTG
    AGTGTCTATG CTCTTAAGGA CACTTTGACA AGCAGACCAG CTCAGGGAGT TGTCACCACT
    CTGGAGAATG TCAGCCCACC AAGAAGGGCT CGTGTGACAG ATGCTACTGA GACCACCATC
    ACCATTAGCT GGAGAACCAA GACTGAGACG ATCACTGGCT TCCAAGTTGA TGCCGTTCCA
    GCCAATGGCC AGACTCCAAT CCAGAGAACC ATCAAGCCAG ATGTCAGAAG CTACACCATC
    ACAGGTTTAC AACCAGGCAC TGACTACAAG ATCTACCTGT ACACCTTGAA TGACAATGCT
    CGGAGCTCCC CTGTGGTCAT CGACGCCTCC ACTGCCATTG ATGCACCATC CAACCTGCGT
    TTCCTGGCCA CCACACCCAA TTCCTTGCTG GTATCATGGC AGCCGCCACG TGCCAGGATT
    ACCGGCTACA TCATCAAGTA TGAGAAGCCT GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACAGA GGCTACTATT ACTGGCCTGG AACCGGGAAC CGAATATACA
    ATTTATGTCA TTGCCCTGAA GAATAATCAG AAGAGCGAGC CCCTGATTGG AAGGAAAAAG
    ACAGGACAAG AAGCTCTCTC TCAGACAACC ATCTCATGGG CCCCATTCCA GGACACTTCT
    GAGTACATCA TTTCATGTCA TCCTGTTGGC ACTGATGAAG AACCCTTACA GTTCAGGGTT
    CCTGGAACTT CTACCAGTGC CACTCTGACA GGCCTCACCA GAGGTGCCAC CTACAACATC
    ATAGTGGAGG CACTGAAAGA CCAGCAGAGG CATAAGGTTC GGGAAGAGGT TGTTACCGTG
    GGCAACTCTG TCAACGAAGG CTTGAACCAA CCTACGGATG ACTCGTGCTT TGACCCCTAC
    ACAGTTTCCC ATTATGCCGT TGGAGATGAG TGGGAACGAA TGTCTGAATC AGGCTTTAAA
    CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT GGTCATTTCA GATGTGATTC ATCTAGATGG
    TGCCATGACA ATGGTGTGAA CTACAAGATT GGAGAGAAGT GGGACCGTCA GGGAGAAAAT
    GGCCAGATGA TGAGCTGCAC ATGTCTTGGG AACGGAAAAG GAGAATTCAA GTGTGACCCT
    CATGAGGCAA CGTGTTATGA TGATGGGAAG ACATACCACG TAGGAGAACA GTGGCAGAAG
    GAATATCTCG GTGCCATTTG CTCCTGCACA TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT
    GACAACTGCC GCAGACCTGG GGGTGAACCC AGTCCCGAAG GCACTACTGG CCAGTCCTAC
    AACCAGTATT CTCAGAGATA CCATCAGAGA ACAAACACTA ATGTTAATTG CCCAATTGAG
    TGCTTCATGC CTTTAGATGT ACAGGCTGAC AGAGAAGATT CCCGAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25005
    Clone ID Related Accession (Same CDS sequence) NM_002026.3
    Accession Version NM_002026.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7068bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Homo sapiens(human)
    Product fibronectin isoform 3 preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX640731.1, AJ535086.1, BX640802.1, X02761.1, AI033037.1, BX640638.1, BQ005645.1 and CR749317.1. On Jan 9, 2016 this sequence version replaced NM_002026.2. Transcript Variant: This variant (3) lacks an in-frame exon and an internal segment in the coding region, as compared to variant 1. Isoform 3 encoded by this variant thus lacks two internal segments, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX538017.1, BX538018.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA
    GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG
    CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC
    AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT
    GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC
    CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT
    CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA
    CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA
    GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG
    ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC
    CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC
    ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG
    TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT
    GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA
    GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC
    AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT
    GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA
    GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG
    AGTCAGCCTC TGGTTCAGAC TGCAGTAACC AACATTGATC GCCCTAAAGG ACTGGCATTC
    ACTGATGTGG ATGTCGATTC CATCAAAATT GCTTGGGAAA GCCCACAGGG GCAAGTTTCC
    AGGTACAGGG TGACCTACTC GAGCCCTGAG GATGGAATCC ATGAGCTATT CCCTGCACCT
    GATGGTGAAG AAGACACTGC AGAGCTGCAA GGCCTCAGAC CGGGTTCTGA GTACACAGTC
    AGTGTGGTTG CCTTGCACGA TGATATGGAG AGCCAGCCCC TGATTGGAAC CCAGTCCACA
    GCTATTCCTG CACCAACTGA CCTGAAGTTC ACTCAGGTCA CACCCACAAG CCTGAGCGCC
    CAGTGGACAC CACCCAATGT TCAGCTCACT GGATATCGAG TGCGGGTGAC CCCCAAGGAG
    AAGACCGGAC CAATGAAAGA AATCAACCTT GCTCCTGACA GCTCATCCGT GGTTGTATCA
    GGACTTATGG TGGCCACCAA ATATGAAGTG AGTGTCTATG CTCTTAAGGA CACTTTGACA
    AGCAGACCAG CTCAGGGAGT TGTCACCACT CTGGAGAATG TCAGCCCACC AAGAAGGGCT
    CGTGTGACAG ATGCTACTGA GACCACCATC ACCATTAGCT GGAGAACCAA GACTGAGACG
    ATCACTGGCT TCCAAGTTGA TGCCGTTCCA GCCAATGGCC AGACTCCAAT CCAGAGAACC
    ATCAAGCCAG ATGTCAGAAG CTACACCATC ACAGGTTTAC AACCAGGCAC TGACTACAAG
    ATCTACCTGT ACACCTTGAA TGACAATGCT CGGAGCTCCC CTGTGGTCAT CGACGCCTCC
    ACTGCCATTG ATGCACCATC CAACCTGCGT TTCCTGGCCA CCACACCCAA TTCCTTGCTG
    GTATCATGGC AGCCGCCACG TGCCAGGATT ACCGGCTACA TCATCAAGTA TGAGAAGCCT
    GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG CCCCGCCCTG GTGTCACAGA GGCTACTATT
    ACTGGCCTGG AACCGGGAAC CGAATATACA ATTTATGTCA TTGCCCTGAA GAATAATCAG
    AAGAGCGAGC CCCTGATTGG AAGGAAAAAG ACAGACGAGC TTCCCCAACT GGTAACCCTT
    CCACACCCCA ATCTTCATGG ACCAGAGATC TTGGATGTTC CTTCCACAGT TCAAAAGACC
    CCTTTCGTCA CCCACCCTGG GTATGACACT GGAAATGGTA TTCAGCTTCC TGGCACTTCT
    GGTCAGCAAC CCAGTGTTGG GCAACAAATG ATCTTTGAGG AACATGGTTT TAGGCGGACC
    ACACCGCCCA CAACGGCCAC CCCCATAAGG CATAGGCCAA GACCATACCC GCCGAATGTA
    GGACAAGAAG CTCTCTCTCA GACAACCATC TCATGGGCCC CATTCCAGGA CACTTCTGAG
    TACATCATTT CATGTCATCC TGTTGGCACT GATGAAGAAC CCTTACAGTT CAGGGTTCCT
    GGAACTTCTA CCAGTGCCAC TCTGACAGGC CTCACCAGAG GTGCCACCTA CAACATCATA
    GTGGAGGCAC TGAAAGACCA GCAGAGGCAT AAGGTTCGGG AAGAGGTTGT TACCGTGGGC
    AACTCTGTCA ACGAAGGCTT GAACCAACCT ACGGATGACT CGTGCTTTGA CCCCTACACA
    GTTTCCCATT ATGCCGTTGG AGATGAGTGG GAACGAATGT CTGAATCAGG CTTTAAACTG
    TTGTGCCAGT GCTTAGGCTT TGGAAGTGGT CATTTCAGAT GTGATTCATC TAGATGGTGC
    CATGACAATG GTGTGAACTA CAAGATTGGA GAGAAGTGGG ACCGTCAGGG AGAAAATGGC
    CAGATGATGA GCTGCACATG TCTTGGGAAC GGAAAAGGAG AATTCAAGTG TGACCCTCAT
    GAGGCAACGT GTTATGATGA TGGGAAGACA TACCACGTAG GAGAACAGTG GCAGAAGGAA
    TATCTCGGTG CCATTTGCTC CTGCACATGC TTTGGAGGCC AGCGGGGCTG GCGCTGTGAC
    AACTGCCGCA GACCTGGGGG TGAACCCAGT CCCGAAGGCA CTACTGGCCA GTCCTACAAC
    CAGTATTCTC AGAGATACCA TCAGAGAACA AACACTAATG TTAATTGCCC AATTGAGTGC
    TTCATGCCTT TAGATGTACA GGCTGACAGA GAAGATTCCC GAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002017.1
    CDS267..7334
    Translation

    Target ORF information:

    RefSeq Version NM_002026.3
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002026.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA
    GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG
    CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC
    AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT
    GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC
    CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT
    CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA
    CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA
    GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG
    ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC
    CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC
    ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG
    TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT
    GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA
    GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC
    AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT
    GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA
    GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG
    AGTCAGCCTC TGGTTCAGAC TGCAGTAACC AACATTGATC GCCCTAAAGG ACTGGCATTC
    ACTGATGTGG ATGTCGATTC CATCAAAATT GCTTGGGAAA GCCCACAGGG GCAAGTTTCC
    AGGTACAGGG TGACCTACTC GAGCCCTGAG GATGGAATCC ATGAGCTATT CCCTGCACCT
    GATGGTGAAG AAGACACTGC AGAGCTGCAA GGCCTCAGAC CGGGTTCTGA GTACACAGTC
    AGTGTGGTTG CCTTGCACGA TGATATGGAG AGCCAGCCCC TGATTGGAAC CCAGTCCACA
    GCTATTCCTG CACCAACTGA CCTGAAGTTC ACTCAGGTCA CACCCACAAG CCTGAGCGCC
    CAGTGGACAC CACCCAATGT TCAGCTCACT GGATATCGAG TGCGGGTGAC CCCCAAGGAG
    AAGACCGGAC CAATGAAAGA AATCAACCTT GCTCCTGACA GCTCATCCGT GGTTGTATCA
    GGACTTATGG TGGCCACCAA ATATGAAGTG AGTGTCTATG CTCTTAAGGA CACTTTGACA
    AGCAGACCAG CTCAGGGAGT TGTCACCACT CTGGAGAATG TCAGCCCACC AAGAAGGGCT
    CGTGTGACAG ATGCTACTGA GACCACCATC ACCATTAGCT GGAGAACCAA GACTGAGACG
    ATCACTGGCT TCCAAGTTGA TGCCGTTCCA GCCAATGGCC AGACTCCAAT CCAGAGAACC
    ATCAAGCCAG ATGTCAGAAG CTACACCATC ACAGGTTTAC AACCAGGCAC TGACTACAAG
    ATCTACCTGT ACACCTTGAA TGACAATGCT CGGAGCTCCC CTGTGGTCAT CGACGCCTCC
    ACTGCCATTG ATGCACCATC CAACCTGCGT TTCCTGGCCA CCACACCCAA TTCCTTGCTG
    GTATCATGGC AGCCGCCACG TGCCAGGATT ACCGGCTACA TCATCAAGTA TGAGAAGCCT
    GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG CCCCGCCCTG GTGTCACAGA GGCTACTATT
    ACTGGCCTGG AACCGGGAAC CGAATATACA ATTTATGTCA TTGCCCTGAA GAATAATCAG
    AAGAGCGAGC CCCTGATTGG AAGGAAAAAG ACAGACGAGC TTCCCCAACT GGTAACCCTT
    CCACACCCCA ATCTTCATGG ACCAGAGATC TTGGATGTTC CTTCCACAGT TCAAAAGACC
    CCTTTCGTCA CCCACCCTGG GTATGACACT GGAAATGGTA TTCAGCTTCC TGGCACTTCT
    GGTCAGCAAC CCAGTGTTGG GCAACAAATG ATCTTTGAGG AACATGGTTT TAGGCGGACC
    ACACCGCCCA CAACGGCCAC CCCCATAAGG CATAGGCCAA GACCATACCC GCCGAATGTA
    GGACAAGAAG CTCTCTCTCA GACAACCATC TCATGGGCCC CATTCCAGGA CACTTCTGAG
    TACATCATTT CATGTCATCC TGTTGGCACT GATGAAGAAC CCTTACAGTT CAGGGTTCCT
    GGAACTTCTA CCAGTGCCAC TCTGACAGGC CTCACCAGAG GTGCCACCTA CAACATCATA
    GTGGAGGCAC TGAAAGACCA GCAGAGGCAT AAGGTTCGGG AAGAGGTTGT TACCGTGGGC
    AACTCTGTCA ACGAAGGCTT GAACCAACCT ACGGATGACT CGTGCTTTGA CCCCTACACA
    GTTTCCCATT ATGCCGTTGG AGATGAGTGG GAACGAATGT CTGAATCAGG CTTTAAACTG
    TTGTGCCAGT GCTTAGGCTT TGGAAGTGGT CATTTCAGAT GTGATTCATC TAGATGGTGC
    CATGACAATG GTGTGAACTA CAAGATTGGA GAGAAGTGGG ACCGTCAGGG AGAAAATGGC
    CAGATGATGA GCTGCACATG TCTTGGGAAC GGAAAAGGAG AATTCAAGTG TGACCCTCAT
    GAGGCAACGT GTTATGATGA TGGGAAGACA TACCACGTAG GAGAACAGTG GCAGAAGGAA
    TATCTCGGTG CCATTTGCTC CTGCACATGC TTTGGAGGCC AGCGGGGCTG GCGCTGTGAC
    AACTGCCGCA GACCTGGGGG TGAACCCAGT CCCGAAGGCA CTACTGGCCA GTCCTACAAC
    CAGTATTCTC AGAGATACCA TCAGAGAACA AACACTAATG TTAATTGCCC AATTGAGTGC
    TTCATGCCTT TAGATGTACA GGCTGACAGA GAAGATTCCC GAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25016
    Clone ID Related Accession (Same CDS sequence) NM_212478.2
    Accession Version NM_212478.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6993bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Homo sapiens(human)
    Product fibronectin isoform 4 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX640802.1, AJ535086.1, X02761.1, AI033037.1, U60068.1, BQ005645.1 and CR749317.1. On Jan 9, 2016 this sequence version replaced NM_212478.1. Transcript Variant: This variant (4) lacks an in-frame exon and two in-frame segments in the coding region, as compared to variant 1. Isoform 4 encoded by this variant thus lacks three internal segments, as compared to isoform 1. The encoded isoform (4) may undergo proteolytic processing similar to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.149860.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA
    GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG
    CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC
    AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT
    GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC
    CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT
    CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA
    CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA
    GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG
    ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC
    CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC
    ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG
    TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT
    GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA
    GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC
    AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT
    GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA
    GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG
    AGTCAGCCTC TGGTTCAGAC TGCAGTAACC AACATTGATC GCCCTAAAGG ACTGGCATTC
    ACTGATGTGG ATGTCGATTC CATCAAAATT GCTTGGGAAA GCCCACAGGG GCAAGTTTCC
    AGGTACAGGG TGACCTACTC GAGCCCTGAG GATGGAATCC ATGAGCTATT CCCTGCACCT
    GATGGTGAAG AAGACACTGC AGAGCTGCAA GGCCTCAGAC CGGGTTCTGA GTACACAGTC
    AGTGTGGTTG CCTTGCACGA TGATATGGAG AGCCAGCCCC TGATTGGAAC CCAGTCCACA
    GCTATTCCTG CACCAACTGA CCTGAAGTTC ACTCAGGTCA CACCCACAAG CCTGAGCGCC
    CAGTGGACAC CACCCAATGT TCAGCTCACT GGATATCGAG TGCGGGTGAC CCCCAAGGAG
    AAGACCGGAC CAATGAAAGA AATCAACCTT GCTCCTGACA GCTCATCCGT GGTTGTATCA
    GGACTTATGG TGGCCACCAA ATATGAAGTG AGTGTCTATG CTCTTAAGGA CACTTTGACA
    AGCAGACCAG CTCAGGGAGT TGTCACCACT CTGGAGAATG TCAGCCCACC AAGAAGGGCT
    CGTGTGACAG ATGCTACTGA GACCACCATC ACCATTAGCT GGAGAACCAA GACTGAGACG
    ATCACTGGCT TCCAAGTTGA TGCCGTTCCA GCCAATGGCC AGACTCCAAT CCAGAGAACC
    ATCAAGCCAG ATGTCAGAAG CTACACCATC ACAGGTTTAC AACCAGGCAC TGACTACAAG
    ATCTACCTGT ACACCTTGAA TGACAATGCT CGGAGCTCCC CTGTGGTCAT CGACGCCTCC
    ACTGCCATTG ATGCACCATC CAACCTGCGT TTCCTGGCCA CCACACCCAA TTCCTTGCTG
    GTATCATGGC AGCCGCCACG TGCCAGGATT ACCGGCTACA TCATCAAGTA TGAGAAGCCT
    GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG CCCCGCCCTG GTGTCACAGA GGCTACTATT
    ACTGGCCTGG AACCGGGAAC CGAATATACA ATTTATGTCA TTGCCCTGAA GAATAATCAG
    AAGAGCGAGC CCCTGATTGG AAGGAAAAAG ACAGTTCAAA AGACCCCTTT CGTCACCCAC
    CCTGGGTATG ACACTGGAAA TGGTATTCAG CTTCCTGGCA CTTCTGGTCA GCAACCCAGT
    GTTGGGCAAC AAATGATCTT TGAGGAACAT GGTTTTAGGC GGACCACACC GCCCACAACG
    GCCACCCCCA TAAGGCATAG GCCAAGACCA TACCCGCCGA ATGTAGGACA AGAAGCTCTC
    TCTCAGACAA CCATCTCATG GGCCCCATTC CAGGACACTT CTGAGTACAT CATTTCATGT
    CATCCTGTTG GCACTGATGA AGAACCCTTA CAGTTCAGGG TTCCTGGAAC TTCTACCAGT
    GCCACTCTGA CAGGCCTCAC CAGAGGTGCC ACCTACAACA TCATAGTGGA GGCACTGAAA
    GACCAGCAGA GGCATAAGGT TCGGGAAGAG GTTGTTACCG TGGGCAACTC TGTCAACGAA
    GGCTTGAACC AACCTACGGA TGACTCGTGC TTTGACCCCT ACACAGTTTC CCATTATGCC
    GTTGGAGATG AGTGGGAACG AATGTCTGAA TCAGGCTTTA AACTGTTGTG CCAGTGCTTA
    GGCTTTGGAA GTGGTCATTT CAGATGTGAT TCATCTAGAT GGTGCCATGA CAATGGTGTG
    AACTACAAGA TTGGAGAGAA GTGGGACCGT CAGGGAGAAA ATGGCCAGAT GATGAGCTGC
    ACATGTCTTG GGAACGGAAA AGGAGAATTC AAGTGTGACC CTCATGAGGC AACGTGTTAT
    GATGATGGGA AGACATACCA CGTAGGAGAA CAGTGGCAGA AGGAATATCT CGGTGCCATT
    TGCTCCTGCA CATGCTTTGG AGGCCAGCGG GGCTGGCGCT GTGACAACTG CCGCAGACCT
    GGGGGTGAAC CCAGTCCCGA AGGCACTACT GGCCAGTCCT ACAACCAGTA TTCTCAGAGA
    TACCATCAGA GAACAAACAC TAATGTTAAT TGCCCAATTG AGTGCTTCAT GCCTTTAGAT
    GTACAGGCTG ACAGAGAAGA TTCCCGAGAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_997643.1
    CDS267..7259
    Translation

    Target ORF information:

    RefSeq Version NM_212478.2
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_212478.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA
    GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG
    CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC
    AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT
    GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC
    CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT
    CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA
    CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA
    GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG
    ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC
    CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC
    ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG
    TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT
    GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA
    GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC
    AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT
    GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA
    GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG
    AGTCAGCCTC TGGTTCAGAC TGCAGTAACC AACATTGATC GCCCTAAAGG ACTGGCATTC
    ACTGATGTGG ATGTCGATTC CATCAAAATT GCTTGGGAAA GCCCACAGGG GCAAGTTTCC
    AGGTACAGGG TGACCTACTC GAGCCCTGAG GATGGAATCC ATGAGCTATT CCCTGCACCT
    GATGGTGAAG AAGACACTGC AGAGCTGCAA GGCCTCAGAC CGGGTTCTGA GTACACAGTC
    AGTGTGGTTG CCTTGCACGA TGATATGGAG AGCCAGCCCC TGATTGGAAC CCAGTCCACA
    GCTATTCCTG CACCAACTGA CCTGAAGTTC ACTCAGGTCA CACCCACAAG CCTGAGCGCC
    CAGTGGACAC CACCCAATGT TCAGCTCACT GGATATCGAG TGCGGGTGAC CCCCAAGGAG
    AAGACCGGAC CAATGAAAGA AATCAACCTT GCTCCTGACA GCTCATCCGT GGTTGTATCA
    GGACTTATGG TGGCCACCAA ATATGAAGTG AGTGTCTATG CTCTTAAGGA CACTTTGACA
    AGCAGACCAG CTCAGGGAGT TGTCACCACT CTGGAGAATG TCAGCCCACC AAGAAGGGCT
    CGTGTGACAG ATGCTACTGA GACCACCATC ACCATTAGCT GGAGAACCAA GACTGAGACG
    ATCACTGGCT TCCAAGTTGA TGCCGTTCCA GCCAATGGCC AGACTCCAAT CCAGAGAACC
    ATCAAGCCAG ATGTCAGAAG CTACACCATC ACAGGTTTAC AACCAGGCAC TGACTACAAG
    ATCTACCTGT ACACCTTGAA TGACAATGCT CGGAGCTCCC CTGTGGTCAT CGACGCCTCC
    ACTGCCATTG ATGCACCATC CAACCTGCGT TTCCTGGCCA CCACACCCAA TTCCTTGCTG
    GTATCATGGC AGCCGCCACG TGCCAGGATT ACCGGCTACA TCATCAAGTA TGAGAAGCCT
    GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG CCCCGCCCTG GTGTCACAGA GGCTACTATT
    ACTGGCCTGG AACCGGGAAC CGAATATACA ATTTATGTCA TTGCCCTGAA GAATAATCAG
    AAGAGCGAGC CCCTGATTGG AAGGAAAAAG ACAGTTCAAA AGACCCCTTT CGTCACCCAC
    CCTGGGTATG ACACTGGAAA TGGTATTCAG CTTCCTGGCA CTTCTGGTCA GCAACCCAGT
    GTTGGGCAAC AAATGATCTT TGAGGAACAT GGTTTTAGGC GGACCACACC GCCCACAACG
    GCCACCCCCA TAAGGCATAG GCCAAGACCA TACCCGCCGA ATGTAGGACA AGAAGCTCTC
    TCTCAGACAA CCATCTCATG GGCCCCATTC CAGGACACTT CTGAGTACAT CATTTCATGT
    CATCCTGTTG GCACTGATGA AGAACCCTTA CAGTTCAGGG TTCCTGGAAC TTCTACCAGT
    GCCACTCTGA CAGGCCTCAC CAGAGGTGCC ACCTACAACA TCATAGTGGA GGCACTGAAA
    GACCAGCAGA GGCATAAGGT TCGGGAAGAG GTTGTTACCG TGGGCAACTC TGTCAACGAA
    GGCTTGAACC AACCTACGGA TGACTCGTGC TTTGACCCCT ACACAGTTTC CCATTATGCC
    GTTGGAGATG AGTGGGAACG AATGTCTGAA TCAGGCTTTA AACTGTTGTG CCAGTGCTTA
    GGCTTTGGAA GTGGTCATTT CAGATGTGAT TCATCTAGAT GGTGCCATGA CAATGGTGTG
    AACTACAAGA TTGGAGAGAA GTGGGACCGT CAGGGAGAAA ATGGCCAGAT GATGAGCTGC
    ACATGTCTTG GGAACGGAAA AGGAGAATTC AAGTGTGACC CTCATGAGGC AACGTGTTAT
    GATGATGGGA AGACATACCA CGTAGGAGAA CAGTGGCAGA AGGAATATCT CGGTGCCATT
    TGCTCCTGCA CATGCTTTGG AGGCCAGCGG GGCTGGCGCT GTGACAACTG CCGCAGACCT
    GGGGGTGAAC CCAGTCCCGA AGGCACTACT GGCCAGTCCT ACAACCAGTA TTCTCAGAGA
    TACCATCAGA GAACAAACAC TAATGTTAAT TGCCCAATTG AGTGCTTCAT GCCTTTAGAT
    GTACAGGCTG ACAGAGAAGA TTCCCGAGAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36020
    Clone ID Related Accession (Same CDS sequence) XM_005246405.1 , NM_001365517.1
    Accession Version NM_001365517.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7164bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product fibronectin isoform 12 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012462.5 and AC073284.5. On Aug 30, 2018 this sequence version replaced XM_005246405.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.254677.1, SRR1803611.47131.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC
    GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG
    GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA
    TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG
    GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG
    ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA
    ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC
    AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG
    CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG
    CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT
    ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT
    CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGACG AGCTTCCCCA ACTGGTAACC
    CTTCCACACC CCAATCTTCA TGGACCAGAG ATCTTGGATG TTCCTTCCAC AGTTCAAAAG
    ACCCCTTTCG TCACCCACCC TGGGTATGAC ACTGGAAATG GTATTCAGCT TCCTGGCACT
    TCTGGTCAGC AACCCAGTGT TGGGCAACAA ATGATCTTTG AGGAACATGG TTTTAGGCGG
    ACCACACCGC CCACAACGGC CACCCCCATA AGGCATAGGC CAAGACCATA CCCGCCGAAT
    GTAGGTGAGG AAATCCAAAT TGGTCACATC CCCAGGGAAG ATGTAGACTA TCACCTGTAC
    CCACACGGTC CGGGACTCAA TCCAAATGCC TCTACAGGAC AAGAAGCTCT CTCTCAGACA
    ACCATCTCAT GGGCCCCATT CCAGGACACT TCTGAGTACA TCATTTCATG TCATCCTGTT
    GGCACTGATG AAGAACCCTT ACAGTTCAGG GTTCCTGGAA CTTCTACCAG TGCCACTCTG
    ACAGGCCTCA CCAGAGGTGC CACCTACAAC GTCATAGTGG AGGCACTGAA AGACCAGCAG
    AGGCATAAGG TTCGGGAAGA GGTTGTTACC GTGGGCAACT CTGTCAACGA AGGCTTGAAC
    CAACCTACGG ATGACTCGTG CTTTGACCCC TACACAGTTT CCCATTATGC CGTTGGAGAT
    GAGTGGGAAC GAATGTCTGA ATCAGGCTTT AAACTGTTGT GCCAGTGCTT AGGCTTTGGA
    AGTGGTCATT TCAGATGTGA TTCATCTAGA TGGTGCCATG ACAATGGTGT GAACTACAAG
    ATTGGAGAGA AGTGGGACCG TCAGGGAGAA AATGGCCAGA TGATGAGCTG CACATGTCTT
    GGGAACGGAA AAGGAGAATT CAAGTGTGAC CCTCATGAGG CAACGTGTTA TGATGATGGG
    AAGACATACC ACGTAGGAGA ACAGTGGCAG AAGGAATATC TCGGTGCCAT TTGCTCCTGC
    ACATGCTTTG GAGGCCAGCG GGGCTGGCGC TGTGACAACT GCCGCAGACC TGGGGGTGAA
    CCCAGTCCCG AAGGCACTAC TGGCCAGTCC TACAACCAGT ATTCTCAGAG ATACCATCAG
    AGAACAAACA CTAATGTTAA TTGCCCAATT GAGTGCTTCA TGCCTTTAGA TGTACAGGCT
    GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001352446.1
    CDS267..7430
    Translation

    Target ORF information:

    RefSeq Version NM_001365517.1
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant 12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001365517.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC
    GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG
    GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA
    TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG
    GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG
    ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA
    ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC
    AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG
    CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG
    CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT
    ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT
    CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGACG AGCTTCCCCA ACTGGTAACC
    CTTCCACACC CCAATCTTCA TGGACCAGAG ATCTTGGATG TTCCTTCCAC AGTTCAAAAG
    ACCCCTTTCG TCACCCACCC TGGGTATGAC ACTGGAAATG GTATTCAGCT TCCTGGCACT
    TCTGGTCAGC AACCCAGTGT TGGGCAACAA ATGATCTTTG AGGAACATGG TTTTAGGCGG
    ACCACACCGC CCACAACGGC CACCCCCATA AGGCATAGGC CAAGACCATA CCCGCCGAAT
    GTAGGTGAGG AAATCCAAAT TGGTCACATC CCCAGGGAAG ATGTAGACTA TCACCTGTAC
    CCACACGGTC CGGGACTCAA TCCAAATGCC TCTACAGGAC AAGAAGCTCT CTCTCAGACA
    ACCATCTCAT GGGCCCCATT CCAGGACACT TCTGAGTACA TCATTTCATG TCATCCTGTT
    GGCACTGATG AAGAACCCTT ACAGTTCAGG GTTCCTGGAA CTTCTACCAG TGCCACTCTG
    ACAGGCCTCA CCAGAGGTGC CACCTACAAC GTCATAGTGG AGGCACTGAA AGACCAGCAG
    AGGCATAAGG TTCGGGAAGA GGTTGTTACC GTGGGCAACT CTGTCAACGA AGGCTTGAAC
    CAACCTACGG ATGACTCGTG CTTTGACCCC TACACAGTTT CCCATTATGC CGTTGGAGAT
    GAGTGGGAAC GAATGTCTGA ATCAGGCTTT AAACTGTTGT GCCAGTGCTT AGGCTTTGGA
    AGTGGTCATT TCAGATGTGA TTCATCTAGA TGGTGCCATG ACAATGGTGT GAACTACAAG
    ATTGGAGAGA AGTGGGACCG TCAGGGAGAA AATGGCCAGA TGATGAGCTG CACATGTCTT
    GGGAACGGAA AAGGAGAATT CAAGTGTGAC CCTCATGAGG CAACGTGTTA TGATGATGGG
    AAGACATACC ACGTAGGAGA ACAGTGGCAG AAGGAATATC TCGGTGCCAT TTGCTCCTGC
    ACATGCTTTG GAGGCCAGCG GGGCTGGCGC TGTGACAACT GCCGCAGACC TGGGGGTGAA
    CCCAGTCCCG AAGGCACTAC TGGCCAGTCC TACAACCAGT ATTCTCAGAG ATACCATCAG
    AGAACAAACA CTAATGTTAA TTGCCCAATT GAGTGCTTCA TGCCTTTAGA TGTACAGGCT
    GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36021
    Clone ID Related Accession (Same CDS sequence) NM_001365518.1 , XM_005246406.1
    Accession Version NM_001365518.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7161bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Homo sapiens(human)
    Product fibronectin isoform 13 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012462.5 and AC073284.5. On Aug 30, 2018 this sequence version replaced XM_005246406.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.203459.1, SRR1660805.45556.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA
    GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG
    CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC
    AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT
    GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC
    CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT
    CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA
    CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA
    GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG
    ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC
    CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC
    ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG
    TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT
    GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA
    GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC
    AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT
    GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA
    GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG
    AGTCAGCCTC TGGTTCAGAC TGCAGTAACC AACATTGATC GCCCTAAAGG ACTGGCATTC
    ACTGATGTGG ATGTCGATTC CATCAAAATT GCTTGGGAAA GCCCACAGGG GCAAGTTTCC
    AGGTACAGGG TGACCTACTC GAGCCCTGAG GATGGAATCC ATGAGCTATT CCCTGCACCT
    GATGGTGAAG AAGACACTGC AGAGCTGCAA GGCCTCAGAC CGGGTTCTGA GTACACAGTC
    AGTGTGGTTG CCTTGCACGA TGATATGGAG AGCCAGCCCC TGATTGGAAC CCAGTCCACA
    GCTATTCCTG CACCAACTGA CCTGAAGTTC ACTCAGGTCA CACCCACAAG CCTGAGCGCC
    CAGTGGACAC CACCCAATGT TCAGCTCACT GGATATCGAG TGCGGGTGAC CCCCAAGGAG
    AAGACCGGAC CAATGAAAGA AATCAACCTT GCTCCTGACA GCTCATCCGT GGTTGTATCA
    GGACTTATGG TGGCCACCAA ATATGAAGTG AGTGTCTATG CTCTTAAGGA CACTTTGACA
    AGCAGACCAG CTCAGGGAGT TGTCACCACT CTGGAGAATG TCAGCCCACC AAGAAGGGCT
    CGTGTGACAG ATGCTACTGA GACCACCATC ACCATTAGCT GGAGAACCAA GACTGAGACG
    ATCACTGGCT TCCAAGTTGA TGCCGTTCCA GCCAATGGCC AGACTCCAAT CCAGAGAACC
    ATCAAGCCAG ATGTCAGAAG CTACACCATC ACAGGTTTAC AACCAGGCAC TGACTACAAG
    ATCTACCTGT ACACCTTGAA TGACAATGCT CGGAGCTCCC CTGTGGTCAT CGACGCCTCC
    ACTGCCATTG ATGCACCATC CAACCTGCGT TTCCTGGCCA CCACACCCAA TTCCTTGCTG
    GTATCATGGC AGCCGCCACG TGCCAGGATT ACCGGCTACA TCATCAAGTA TGAGAAGCCT
    GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG CCCCGCCCTG GTGTCACAGA GGCTACTATT
    ACTGGCCTGG AACCGGGAAC CGAATATACA ATTTATGTCA TTGCCCTGAA GAATAATCAG
    AAGAGCGAGC CCCTGATTGG AAGGAAAAAG ACAGACGAGC TTCCCCAACT GGTAACCCTT
    CCACACCCCA ATCTTCATGG ACCAGAGATC TTGGATGTTC CTTCCACAGT TCAAAAGACC
    CCTTTCGTCA CCCACCCTGG GTATGACACT GGAAATGGTA TTCAGCTTCC TGGCACTTCT
    GGTCAGCAAC CCAGTGTTGG GCAACAAATG ATCTTTGAGG AACATGGTTT TAGGCGGACC
    ACACCGCCCA CAACGGCCAC CCCCATAAGG CATAGGCCAA GACCATACCC GCCGAATGTA
    GGTGAGGAAA TCCAAATTGG TCACATCCCC AGGGAAGATG TAGACTATCA CCTGTACCCA
    CACGGTCCGG GACTCAATCC AAATGCCTCT ACAGGACAAG AAGCTCTCTC TCAGACAACC
    ATCTCATGGG CCCCATTCCA GGACACTTCT GAGTACATCA TTTCATGTCA TCCTGTTGGC
    ACTGATGAAG AACCCTTACA GTTCAGGGTT CCTGGAACTT CTACCAGTGC CACTCTGACA
    GGCCTCACCA GAGGTGCCAC CTACAACGTC ATAGTGGAGG CACTGAAAGA CCAGCAGAGG
    CATAAGGTTC GGGAAGAGGT TGTTACCGTG GGCAACTCTG TCAACGAAGG CTTGAACCAA
    CCTACGGATG ACTCGTGCTT TGACCCCTAC ACAGTTTCCC ATTATGCCGT TGGAGATGAG
    TGGGAACGAA TGTCTGAATC AGGCTTTAAA CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT
    GGTCATTTCA GATGTGATTC ATCTAGATGG TGCCATGACA ATGGTGTGAA CTACAAGATT
    GGAGAGAAGT GGGACCGTCA GGGAGAAAAT GGCCAGATGA TGAGCTGCAC ATGTCTTGGG
    AACGGAAAAG GAGAATTCAA GTGTGACCCT CATGAGGCAA CGTGTTATGA TGATGGGAAG
    ACATACCACG TAGGAGAACA GTGGCAGAAG GAATATCTCG GTGCCATTTG CTCCTGCACA
    TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT GACAACTGCC GCAGACCTGG GGGTGAACCC
    AGTCCCGAAG GCACTACTGG CCAGTCCTAC AACCAGTATT CTCAGAGATA CCATCAGAGA
    ACAAACACTA ATGTTAATTG CCCAATTGAG TGCTTCATGC CTTTAGATGT ACAGGCTGAC
    AGAGAAGATT CCCGAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001352447.1
    CDS267..7427
    Translation

    Target ORF information:

    RefSeq Version NM_001365518.1
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant 13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001365518.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA
    GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG
    CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC
    AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT
    GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC
    CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT
    CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA
    CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA
    GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG
    ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC
    CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC
    ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG
    TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT
    GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA
    GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC
    AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT
    GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA
    GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG
    AGTCAGCCTC TGGTTCAGAC TGCAGTAACC AACATTGATC GCCCTAAAGG ACTGGCATTC
    ACTGATGTGG ATGTCGATTC CATCAAAATT GCTTGGGAAA GCCCACAGGG GCAAGTTTCC
    AGGTACAGGG TGACCTACTC GAGCCCTGAG GATGGAATCC ATGAGCTATT CCCTGCACCT
    GATGGTGAAG AAGACACTGC AGAGCTGCAA GGCCTCAGAC CGGGTTCTGA GTACACAGTC
    AGTGTGGTTG CCTTGCACGA TGATATGGAG AGCCAGCCCC TGATTGGAAC CCAGTCCACA
    GCTATTCCTG CACCAACTGA CCTGAAGTTC ACTCAGGTCA CACCCACAAG CCTGAGCGCC
    CAGTGGACAC CACCCAATGT TCAGCTCACT GGATATCGAG TGCGGGTGAC CCCCAAGGAG
    AAGACCGGAC CAATGAAAGA AATCAACCTT GCTCCTGACA GCTCATCCGT GGTTGTATCA
    GGACTTATGG TGGCCACCAA ATATGAAGTG AGTGTCTATG CTCTTAAGGA CACTTTGACA
    AGCAGACCAG CTCAGGGAGT TGTCACCACT CTGGAGAATG TCAGCCCACC AAGAAGGGCT
    CGTGTGACAG ATGCTACTGA GACCACCATC ACCATTAGCT GGAGAACCAA GACTGAGACG
    ATCACTGGCT TCCAAGTTGA TGCCGTTCCA GCCAATGGCC AGACTCCAAT CCAGAGAACC
    ATCAAGCCAG ATGTCAGAAG CTACACCATC ACAGGTTTAC AACCAGGCAC TGACTACAAG
    ATCTACCTGT ACACCTTGAA TGACAATGCT CGGAGCTCCC CTGTGGTCAT CGACGCCTCC
    ACTGCCATTG ATGCACCATC CAACCTGCGT TTCCTGGCCA CCACACCCAA TTCCTTGCTG
    GTATCATGGC AGCCGCCACG TGCCAGGATT ACCGGCTACA TCATCAAGTA TGAGAAGCCT
    GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG CCCCGCCCTG GTGTCACAGA GGCTACTATT
    ACTGGCCTGG AACCGGGAAC CGAATATACA ATTTATGTCA TTGCCCTGAA GAATAATCAG
    AAGAGCGAGC CCCTGATTGG AAGGAAAAAG ACAGACGAGC TTCCCCAACT GGTAACCCTT
    CCACACCCCA ATCTTCATGG ACCAGAGATC TTGGATGTTC CTTCCACAGT TCAAAAGACC
    CCTTTCGTCA CCCACCCTGG GTATGACACT GGAAATGGTA TTCAGCTTCC TGGCACTTCT
    GGTCAGCAAC CCAGTGTTGG GCAACAAATG ATCTTTGAGG AACATGGTTT TAGGCGGACC
    ACACCGCCCA CAACGGCCAC CCCCATAAGG CATAGGCCAA GACCATACCC GCCGAATGTA
    GGTGAGGAAA TCCAAATTGG TCACATCCCC AGGGAAGATG TAGACTATCA CCTGTACCCA
    CACGGTCCGG GACTCAATCC AAATGCCTCT ACAGGACAAG AAGCTCTCTC TCAGACAACC
    ATCTCATGGG CCCCATTCCA GGACACTTCT GAGTACATCA TTTCATGTCA TCCTGTTGGC
    ACTGATGAAG AACCCTTACA GTTCAGGGTT CCTGGAACTT CTACCAGTGC CACTCTGACA
    GGCCTCACCA GAGGTGCCAC CTACAACGTC ATAGTGGAGG CACTGAAAGA CCAGCAGAGG
    CATAAGGTTC GGGAAGAGGT TGTTACCGTG GGCAACTCTG TCAACGAAGG CTTGAACCAA
    CCTACGGATG ACTCGTGCTT TGACCCCTAC ACAGTTTCCC ATTATGCCGT TGGAGATGAG
    TGGGAACGAA TGTCTGAATC AGGCTTTAAA CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT
    GGTCATTTCA GATGTGATTC ATCTAGATGG TGCCATGACA ATGGTGTGAA CTACAAGATT
    GGAGAGAAGT GGGACCGTCA GGGAGAAAAT GGCCAGATGA TGAGCTGCAC ATGTCTTGGG
    AACGGAAAAG GAGAATTCAA GTGTGACCCT CATGAGGCAA CGTGTTATGA TGATGGGAAG
    ACATACCACG TAGGAGAACA GTGGCAGAAG GAATATCTCG GTGCCATTTG CTCCTGCACA
    TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT GACAACTGCC GCAGACCTGG GGGTGAACCC
    AGTCCCGAAG GCACTACTGG CCAGTCCTAC AACCAGTATT CTCAGAGATA CCATCAGAGA
    ACAAACACTA ATGTTAATTG CCCAATTGAG TGCTTCATGC CTTTAGATGT ACAGGCTGAC
    AGAGAAGATT CCCGAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36024
    Clone ID Related Accession (Same CDS sequence) NM_001365520.1 , XM_005246409.1
    Accession Version NM_001365520.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7086bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product fibronectin isoform 15 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012462.5 and AC073284.5. On Aug 30, 2018 this sequence version replaced XM_005246409.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.190776.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA
    GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG
    CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC
    AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT
    GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC
    CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT
    CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA
    CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA
    GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG
    ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC
    CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC
    ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG
    TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT
    GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA
    GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC
    AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT
    GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA
    GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG
    AGTCAGCCTC TGGTTCAGAC TGCAGTAACC AACATTGATC GCCCTAAAGG ACTGGCATTC
    ACTGATGTGG ATGTCGATTC CATCAAAATT GCTTGGGAAA GCCCACAGGG GCAAGTTTCC
    AGGTACAGGG TGACCTACTC GAGCCCTGAG GATGGAATCC ATGAGCTATT CCCTGCACCT
    GATGGTGAAG AAGACACTGC AGAGCTGCAA GGCCTCAGAC CGGGTTCTGA GTACACAGTC
    AGTGTGGTTG CCTTGCACGA TGATATGGAG AGCCAGCCCC TGATTGGAAC CCAGTCCACA
    GCTATTCCTG CACCAACTGA CCTGAAGTTC ACTCAGGTCA CACCCACAAG CCTGAGCGCC
    CAGTGGACAC CACCCAATGT TCAGCTCACT GGATATCGAG TGCGGGTGAC CCCCAAGGAG
    AAGACCGGAC CAATGAAAGA AATCAACCTT GCTCCTGACA GCTCATCCGT GGTTGTATCA
    GGACTTATGG TGGCCACCAA ATATGAAGTG AGTGTCTATG CTCTTAAGGA CACTTTGACA
    AGCAGACCAG CTCAGGGAGT TGTCACCACT CTGGAGAATG TCAGCCCACC AAGAAGGGCT
    CGTGTGACAG ATGCTACTGA GACCACCATC ACCATTAGCT GGAGAACCAA GACTGAGACG
    ATCACTGGCT TCCAAGTTGA TGCCGTTCCA GCCAATGGCC AGACTCCAAT CCAGAGAACC
    ATCAAGCCAG ATGTCAGAAG CTACACCATC ACAGGTTTAC AACCAGGCAC TGACTACAAG
    ATCTACCTGT ACACCTTGAA TGACAATGCT CGGAGCTCCC CTGTGGTCAT CGACGCCTCC
    ACTGCCATTG ATGCACCATC CAACCTGCGT TTCCTGGCCA CCACACCCAA TTCCTTGCTG
    GTATCATGGC AGCCGCCACG TGCCAGGATT ACCGGCTACA TCATCAAGTA TGAGAAGCCT
    GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG CCCCGCCCTG GTGTCACAGA GGCTACTATT
    ACTGGCCTGG AACCGGGAAC CGAATATACA ATTTATGTCA TTGCCCTGAA GAATAATCAG
    AAGAGCGAGC CCCTGATTGG AAGGAAAAAG ACAGTTCAAA AGACCCCTTT CGTCACCCAC
    CCTGGGTATG ACACTGGAAA TGGTATTCAG CTTCCTGGCA CTTCTGGTCA GCAACCCAGT
    GTTGGGCAAC AAATGATCTT TGAGGAACAT GGTTTTAGGC GGACCACACC GCCCACAACG
    GCCACCCCCA TAAGGCATAG GCCAAGACCA TACCCGCCGA ATGTAGGTGA GGAAATCCAA
    ATTGGTCACA TCCCCAGGGA AGATGTAGAC TATCACCTGT ACCCACACGG TCCGGGACTC
    AATCCAAATG CCTCTACAGG ACAAGAAGCT CTCTCTCAGA CAACCATCTC ATGGGCCCCA
    TTCCAGGACA CTTCTGAGTA CATCATTTCA TGTCATCCTG TTGGCACTGA TGAAGAACCC
    TTACAGTTCA GGGTTCCTGG AACTTCTACC AGTGCCACTC TGACAGGCCT CACCAGAGGT
    GCCACCTACA ACGTCATAGT GGAGGCACTG AAAGACCAGC AGAGGCATAA GGTTCGGGAA
    GAGGTTGTTA CCGTGGGCAA CTCTGTCAAC GAAGGCTTGA ACCAACCTAC GGATGACTCG
    TGCTTTGACC CCTACACAGT TTCCCATTAT GCCGTTGGAG ATGAGTGGGA ACGAATGTCT
    GAATCAGGCT TTAAACTGTT GTGCCAGTGC TTAGGCTTTG GAAGTGGTCA TTTCAGATGT
    GATTCATCTA GATGGTGCCA TGACAATGGT GTGAACTACA AGATTGGAGA GAAGTGGGAC
    CGTCAGGGAG AAAATGGCCA GATGATGAGC TGCACATGTC TTGGGAACGG AAAAGGAGAA
    TTCAAGTGTG ACCCTCATGA GGCAACGTGT TATGATGATG GGAAGACATA CCACGTAGGA
    GAACAGTGGC AGAAGGAATA TCTCGGTGCC ATTTGCTCCT GCACATGCTT TGGAGGCCAG
    CGGGGCTGGC GCTGTGACAA CTGCCGCAGA CCTGGGGGTG AACCCAGTCC CGAAGGCACT
    ACTGGCCAGT CCTACAACCA GTATTCTCAG AGATACCATC AGAGAACAAA CACTAATGTT
    AATTGCCCAA TTGAGTGCTT CATGCCTTTA GATGTACAGG CTGACAGAGA AGATTCCCGA
    GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001352449.1
    CDS267..7352
    Translation

    Target ORF information:

    RefSeq Version NM_001365520.1
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant 15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001365520.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA
    GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG
    CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC
    AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT
    GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC
    CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT
    CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA
    CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA
    GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG
    ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC
    CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC
    ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG
    TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT
    GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA
    GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC
    AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT
    GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA
    GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG
    AGTCAGCCTC TGGTTCAGAC TGCAGTAACC AACATTGATC GCCCTAAAGG ACTGGCATTC
    ACTGATGTGG ATGTCGATTC CATCAAAATT GCTTGGGAAA GCCCACAGGG GCAAGTTTCC
    AGGTACAGGG TGACCTACTC GAGCCCTGAG GATGGAATCC ATGAGCTATT CCCTGCACCT
    GATGGTGAAG AAGACACTGC AGAGCTGCAA GGCCTCAGAC CGGGTTCTGA GTACACAGTC
    AGTGTGGTTG CCTTGCACGA TGATATGGAG AGCCAGCCCC TGATTGGAAC CCAGTCCACA
    GCTATTCCTG CACCAACTGA CCTGAAGTTC ACTCAGGTCA CACCCACAAG CCTGAGCGCC
    CAGTGGACAC CACCCAATGT TCAGCTCACT GGATATCGAG TGCGGGTGAC CCCCAAGGAG
    AAGACCGGAC CAATGAAAGA AATCAACCTT GCTCCTGACA GCTCATCCGT GGTTGTATCA
    GGACTTATGG TGGCCACCAA ATATGAAGTG AGTGTCTATG CTCTTAAGGA CACTTTGACA
    AGCAGACCAG CTCAGGGAGT TGTCACCACT CTGGAGAATG TCAGCCCACC AAGAAGGGCT
    CGTGTGACAG ATGCTACTGA GACCACCATC ACCATTAGCT GGAGAACCAA GACTGAGACG
    ATCACTGGCT TCCAAGTTGA TGCCGTTCCA GCCAATGGCC AGACTCCAAT CCAGAGAACC
    ATCAAGCCAG ATGTCAGAAG CTACACCATC ACAGGTTTAC AACCAGGCAC TGACTACAAG
    ATCTACCTGT ACACCTTGAA TGACAATGCT CGGAGCTCCC CTGTGGTCAT CGACGCCTCC
    ACTGCCATTG ATGCACCATC CAACCTGCGT TTCCTGGCCA CCACACCCAA TTCCTTGCTG
    GTATCATGGC AGCCGCCACG TGCCAGGATT ACCGGCTACA TCATCAAGTA TGAGAAGCCT
    GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG CCCCGCCCTG GTGTCACAGA GGCTACTATT
    ACTGGCCTGG AACCGGGAAC CGAATATACA ATTTATGTCA TTGCCCTGAA GAATAATCAG
    AAGAGCGAGC CCCTGATTGG AAGGAAAAAG ACAGTTCAAA AGACCCCTTT CGTCACCCAC
    CCTGGGTATG ACACTGGAAA TGGTATTCAG CTTCCTGGCA CTTCTGGTCA GCAACCCAGT
    GTTGGGCAAC AAATGATCTT TGAGGAACAT GGTTTTAGGC GGACCACACC GCCCACAACG
    GCCACCCCCA TAAGGCATAG GCCAAGACCA TACCCGCCGA ATGTAGGTGA GGAAATCCAA
    ATTGGTCACA TCCCCAGGGA AGATGTAGAC TATCACCTGT ACCCACACGG TCCGGGACTC
    AATCCAAATG CCTCTACAGG ACAAGAAGCT CTCTCTCAGA CAACCATCTC ATGGGCCCCA
    TTCCAGGACA CTTCTGAGTA CATCATTTCA TGTCATCCTG TTGGCACTGA TGAAGAACCC
    TTACAGTTCA GGGTTCCTGG AACTTCTACC AGTGCCACTC TGACAGGCCT CACCAGAGGT
    GCCACCTACA ACGTCATAGT GGAGGCACTG AAAGACCAGC AGAGGCATAA GGTTCGGGAA
    GAGGTTGTTA CCGTGGGCAA CTCTGTCAAC GAAGGCTTGA ACCAACCTAC GGATGACTCG
    TGCTTTGACC CCTACACAGT TTCCCATTAT GCCGTTGGAG ATGAGTGGGA ACGAATGTCT
    GAATCAGGCT TTAAACTGTT GTGCCAGTGC TTAGGCTTTG GAAGTGGTCA TTTCAGATGT
    GATTCATCTA GATGGTGCCA TGACAATGGT GTGAACTACA AGATTGGAGA GAAGTGGGAC
    CGTCAGGGAG AAAATGGCCA GATGATGAGC TGCACATGTC TTGGGAACGG AAAAGGAGAA
    TTCAAGTGTG ACCCTCATGA GGCAACGTGT TATGATGATG GGAAGACATA CCACGTAGGA
    GAACAGTGGC AGAAGGAATA TCTCGGTGCC ATTTGCTCCT GCACATGCTT TGGAGGCCAG
    CGGGGCTGGC GCTGTGACAA CTGCCGCAGA CCTGGGGGTG AACCCAGTCC CGAAGGCACT
    ACTGGCCAGT CCTACAACCA GTATTCTCAG AGATACCATC AGAGAACAAA CACTAATGTT
    AATTGCCCAA TTGAGTGCTT CATGCCTTTA GATGTACAGG CTGACAGAGA AGATTCCCGA
    GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36027
    Clone ID Related Accession (Same CDS sequence) XM_005246412.1 , NM_001365523.1
    Accession Version NM_001365523.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6816bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-29
    Organism Homo sapiens(human)
    Product fibronectin isoform 18 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012462.5 and AC073284.5. On Aug 30, 2018 this sequence version replaced XM_005246412.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.187123.1, SRR1660809.155143.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA
    GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG
    CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC
    AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT
    GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC
    CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT
    CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA
    CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA
    GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG
    ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC
    CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC
    ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG
    TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT
    GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA
    GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC
    AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT
    GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA
    GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG
    AGTCAGCCTC TGGTTCAGAC TGCAGTAACC ACTATTCCTG CACCAACTGA CCTGAAGTTC
    ACTCAGGTCA CACCCACAAG CCTGAGCGCC CAGTGGACAC CACCCAATGT TCAGCTCACT
    GGATATCGAG TGCGGGTGAC CCCCAAGGAG AAGACCGGAC CAATGAAAGA AATCAACCTT
    GCTCCTGACA GCTCATCCGT GGTTGTATCA GGACTTATGG TGGCCACCAA ATATGAAGTG
    AGTGTCTATG CTCTTAAGGA CACTTTGACA AGCAGACCAG CTCAGGGAGT TGTCACCACT
    CTGGAGAATG TCAGCCCACC AAGAAGGGCT CGTGTGACAG ATGCTACTGA GACCACCATC
    ACCATTAGCT GGAGAACCAA GACTGAGACG ATCACTGGCT TCCAAGTTGA TGCCGTTCCA
    GCCAATGGCC AGACTCCAAT CCAGAGAACC ATCAAGCCAG ATGTCAGAAG CTACACCATC
    ACAGGTTTAC AACCAGGCAC TGACTACAAG ATCTACCTGT ACACCTTGAA TGACAATGCT
    CGGAGCTCCC CTGTGGTCAT CGACGCCTCC ACTGCCATTG ATGCACCATC CAACCTGCGT
    TTCCTGGCCA CCACACCCAA TTCCTTGCTG GTATCATGGC AGCCGCCACG TGCCAGGATT
    ACCGGCTACA TCATCAAGTA TGAGAAGCCT GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACAGA GGCTACTATT ACTGGCCTGG AACCGGGAAC CGAATATACA
    ATTTATGTCA TTGCCCTGAA GAATAATCAG AAGAGCGAGC CCCTGATTGG AAGGAAAAAG
    ACAGTTCAAA AGACCCCTTT CGTCACCCAC CCTGGGTATG ACACTGGAAA TGGTATTCAG
    CTTCCTGGCA CTTCTGGTCA GCAACCCAGT GTTGGGCAAC AAATGATCTT TGAGGAACAT
    GGTTTTAGGC GGACCACACC GCCCACAACG GCCACCCCCA TAAGGCATAG GCCAAGACCA
    TACCCGCCGA ATGTAGGTGA GGAAATCCAA ATTGGTCACA TCCCCAGGGA AGATGTAGAC
    TATCACCTGT ACCCACACGG TCCGGGACTC AATCCAAATG CCTCTACAGG ACAAGAAGCT
    CTCTCTCAGA CAACCATCTC ATGGGCCCCA TTCCAGGACA CTTCTGAGTA CATCATTTCA
    TGTCATCCTG TTGGCACTGA TGAAGAACCC TTACAGTTCA GGGTTCCTGG AACTTCTACC
    AGTGCCACTC TGACAGGCCT CACCAGAGGT GCCACCTACA ACGTCATAGT GGAGGCACTG
    AAAGACCAGC AGAGGCATAA GGTTCGGGAA GAGGTTGTTA CCGTGGGCAA CTCTGTCAAC
    GAAGGCTTGA ACCAACCTAC GGATGACTCG TGCTTTGACC CCTACACAGT TTCCCATTAT
    GCCGTTGGAG ATGAGTGGGA ACGAATGTCT GAATCAGGCT TTAAACTGTT GTGCCAGTGC
    TTAGGCTTTG GAAGTGGTCA TTTCAGATGT GATTCATCTA GATGGTGCCA TGACAATGGT
    GTGAACTACA AGATTGGAGA GAAGTGGGAC CGTCAGGGAG AAAATGGCCA GATGATGAGC
    TGCACATGTC TTGGGAACGG AAAAGGAGAA TTCAAGTGTG ACCCTCATGA GGCAACGTGT
    TATGATGATG GGAAGACATA CCACGTAGGA GAACAGTGGC AGAAGGAATA TCTCGGTGCC
    ATTTGCTCCT GCACATGCTT TGGAGGCCAG CGGGGCTGGC GCTGTGACAA CTGCCGCAGA
    CCTGGGGGTG AACCCAGTCC CGAAGGCACT ACTGGCCAGT CCTACAACCA GTATTCTCAG
    AGATACCATC AGAGAACAAA CACTAATGTT AATTGCCCAA TTGAGTGCTT CATGCCTTTA
    GATGTACAGG CTGACAGAGA AGATTCCCGA GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001352452.1
    CDS267..7082
    Translation

    Target ORF information:

    RefSeq Version NM_001365523.1
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant 18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001365523.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA
    GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG
    CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC
    AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT
    GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC
    CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT
    CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA
    CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA
    GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG
    ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC
    CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC
    ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG
    TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT
    GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA
    GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC
    AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT
    GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA
    GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG
    AGTCAGCCTC TGGTTCAGAC TGCAGTAACC ACTATTCCTG CACCAACTGA CCTGAAGTTC
    ACTCAGGTCA CACCCACAAG CCTGAGCGCC CAGTGGACAC CACCCAATGT TCAGCTCACT
    GGATATCGAG TGCGGGTGAC CCCCAAGGAG AAGACCGGAC CAATGAAAGA AATCAACCTT
    GCTCCTGACA GCTCATCCGT GGTTGTATCA GGACTTATGG TGGCCACCAA ATATGAAGTG
    AGTGTCTATG CTCTTAAGGA CACTTTGACA AGCAGACCAG CTCAGGGAGT TGTCACCACT
    CTGGAGAATG TCAGCCCACC AAGAAGGGCT CGTGTGACAG ATGCTACTGA GACCACCATC
    ACCATTAGCT GGAGAACCAA GACTGAGACG ATCACTGGCT TCCAAGTTGA TGCCGTTCCA
    GCCAATGGCC AGACTCCAAT CCAGAGAACC ATCAAGCCAG ATGTCAGAAG CTACACCATC
    ACAGGTTTAC AACCAGGCAC TGACTACAAG ATCTACCTGT ACACCTTGAA TGACAATGCT
    CGGAGCTCCC CTGTGGTCAT CGACGCCTCC ACTGCCATTG ATGCACCATC CAACCTGCGT
    TTCCTGGCCA CCACACCCAA TTCCTTGCTG GTATCATGGC AGCCGCCACG TGCCAGGATT
    ACCGGCTACA TCATCAAGTA TGAGAAGCCT GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACAGA GGCTACTATT ACTGGCCTGG AACCGGGAAC CGAATATACA
    ATTTATGTCA TTGCCCTGAA GAATAATCAG AAGAGCGAGC CCCTGATTGG AAGGAAAAAG
    ACAGTTCAAA AGACCCCTTT CGTCACCCAC CCTGGGTATG ACACTGGAAA TGGTATTCAG
    CTTCCTGGCA CTTCTGGTCA GCAACCCAGT GTTGGGCAAC AAATGATCTT TGAGGAACAT
    GGTTTTAGGC GGACCACACC GCCCACAACG GCCACCCCCA TAAGGCATAG GCCAAGACCA
    TACCCGCCGA ATGTAGGTGA GGAAATCCAA ATTGGTCACA TCCCCAGGGA AGATGTAGAC
    TATCACCTGT ACCCACACGG TCCGGGACTC AATCCAAATG CCTCTACAGG ACAAGAAGCT
    CTCTCTCAGA CAACCATCTC ATGGGCCCCA TTCCAGGACA CTTCTGAGTA CATCATTTCA
    TGTCATCCTG TTGGCACTGA TGAAGAACCC TTACAGTTCA GGGTTCCTGG AACTTCTACC
    AGTGCCACTC TGACAGGCCT CACCAGAGGT GCCACCTACA ACGTCATAGT GGAGGCACTG
    AAAGACCAGC AGAGGCATAA GGTTCGGGAA GAGGTTGTTA CCGTGGGCAA CTCTGTCAAC
    GAAGGCTTGA ACCAACCTAC GGATGACTCG TGCTTTGACC CCTACACAGT TTCCCATTAT
    GCCGTTGGAG ATGAGTGGGA ACGAATGTCT GAATCAGGCT TTAAACTGTT GTGCCAGTGC
    TTAGGCTTTG GAAGTGGTCA TTTCAGATGT GATTCATCTA GATGGTGCCA TGACAATGGT
    GTGAACTACA AGATTGGAGA GAAGTGGGAC CGTCAGGGAG AAAATGGCCA GATGATGAGC
    TGCACATGTC TTGGGAACGG AAAAGGAGAA TTCAAGTGTG ACCCTCATGA GGCAACGTGT
    TATGATGATG GGAAGACATA CCACGTAGGA GAACAGTGGC AGAAGGAATA TCTCGGTGCC
    ATTTGCTCCT GCACATGCTT TGGAGGCCAG CGGGGCTGGC GCTGTGACAA CTGCCGCAGA
    CCTGGGGGTG AACCCAGTCC CGAAGGCACT ACTGGCCAGT CCTACAACCA GTATTCTCAG
    AGATACCATC AGAGAACAAA CACTAATGTT AATTGCCCAA TTGAGTGCTT CATGCCTTTA
    GATGTACAGG CTGACAGAGA AGATTCCCGA GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36029
    Clone ID Related Accession (Same CDS sequence) NM_001365524.1 , XM_005246414.1
    Accession Version NM_001365524.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6801bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product fibronectin isoform 19 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012462.5 and AC073284.5. On Aug 30, 2018 this sequence version replaced XM_005246414.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.245537.1, SRR7346977.2900646.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA
    GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG
    CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC
    AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT
    GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC
    CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT
    CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA
    CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA
    GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG
    ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC
    CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC
    ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG
    TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT
    GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA
    GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC
    AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT
    GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA
    GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG
    AGTCAGCCTC TGGTTCAGAC TGCAGTAACC AACATTGATC GCCCTAAAGG ACTGGCATTC
    ACTGATGTGG ATGTCGATTC CATCAAAATT GCTTGGGAAA GCCCACAGGG GCAAGTTTCC
    AGGTACAGGG TGACCTACTC GAGCCCTGAG GATGGAATCC ATGAGCTATT CCCTGCACCT
    GATGGTGAAG AAGACACTGC AGAGCTGCAA GGCCTCAGAC CGGGTTCTGA GTACACAGTC
    AGTGTGGTTG CCTTGCACGA TGATATGGAG AGCCAGCCCC TGATTGGAAC CCAGTCCACA
    GCTATTCCTG CACCAACTGA CCTGAAGTTC ACTCAGGTCA CACCCACAAG CCTGAGCGCC
    CAGTGGACAC CACCCAATGT TCAGCTCACT GGATATCGAG TGCGGGTGAC CCCCAAGGAG
    AAGACCGGAC CAATGAAAGA AATCAACCTT GCTCCTGACA GCTCATCCGT GGTTGTATCA
    GGACTTATGG TGGCCACCAA ATATGAAGTG AGTGTCTATG CTCTTAAGGA CACTTTGACA
    AGCAGACCAG CTCAGGGAGT TGTCACCACT CTGGAGAATG TCAGCCCACC AAGAAGGGCT
    CGTGTGACAG ATGCTACTGA GACCACCATC ACCATTAGCT GGAGAACCAA GACTGAGACG
    ATCACTGGCT TCCAAGTTGA TGCCGTTCCA GCCAATGGCC AGACTCCAAT CCAGAGAACC
    ATCAAGCCAG ATGTCAGAAG CTACACCATC ACAGGTTTAC AACCAGGCAC TGACTACAAG
    ATCTACCTGT ACACCTTGAA TGACAATGCT CGGAGCTCCC CTGTGGTCAT CGACGCCTCC
    ACTGCCATTG ATGCACCATC CAACCTGCGT TTCCTGGCCA CCACACCCAA TTCCTTGCTG
    GTATCATGGC AGCCGCCACG TGCCAGGATT ACCGGCTACA TCATCAAGTA TGAGAAGCCT
    GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG CCCCGCCCTG GTGTCACAGA GGCTACTATT
    ACTGGCCTGG AACCGGGAAC CGAATATACA ATTTATGTCA TTGCCCTGAA GAATAATCAG
    AAGAGCGAGC CCCTGATTGG AAGGAAAAAG ACAGGACAAG AAGCTCTCTC TCAGACAACC
    ATCTCATGGG CCCCATTCCA GGACACTTCT GAGTACATCA TTTCATGTCA TCCTGTTGGC
    ACTGATGAAG AACCCTTACA GTTCAGGGTT CCTGGAACTT CTACCAGTGC CACTCTGACA
    GGCCTCACCA GAGGTGCCAC CTACAACGTC ATAGTGGAGG CACTGAAAGA CCAGCAGAGG
    CATAAGGTTC GGGAAGAGGT TGTTACCGTG GGCAACTCTG TCAACGAAGG CTTGAACCAA
    CCTACGGATG ACTCGTGCTT TGACCCCTAC ACAGTTTCCC ATTATGCCGT TGGAGATGAG
    TGGGAACGAA TGTCTGAATC AGGCTTTAAA CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT
    GGTCATTTCA GATGTGATTC ATCTAGATGG TGCCATGACA ATGGTGTGAA CTACAAGATT
    GGAGAGAAGT GGGACCGTCA GGGAGAAAAT GGCCAGATGA TGAGCTGCAC ATGTCTTGGG
    AACGGAAAAG GAGAATTCAA GTGTGACCCT CATGAGGCAA CGTGTTATGA TGATGGGAAG
    ACATACCACG TAGGAGAACA GTGGCAGAAG GAATATCTCG GTGCCATTTG CTCCTGCACA
    TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT GACAACTGCC GCAGACCTGG GGGTGAACCC
    AGTCCCGAAG GCACTACTGG CCAGTCCTAC AACCAGTATT CTCAGAGATA CCATCAGAGA
    ACAAACACTA ATGTTAATTG CCCAATTGAG TGCTTCATGC CTTTAGATGT ACAGGCTGAC
    AGAGAAGATT CCCGAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001352453.1
    CDS267..7067
    Translation

    Target ORF information:

    RefSeq Version NM_001365524.1
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant 19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001365524.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA
    GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG
    CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC
    AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT
    GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC
    CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT
    CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA
    CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA
    GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG
    ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC
    CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC
    ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG
    TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT
    GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA
    GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC
    AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT
    GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA
    GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG
    AGTCAGCCTC TGGTTCAGAC TGCAGTAACC AACATTGATC GCCCTAAAGG ACTGGCATTC
    ACTGATGTGG ATGTCGATTC CATCAAAATT GCTTGGGAAA GCCCACAGGG GCAAGTTTCC
    AGGTACAGGG TGACCTACTC GAGCCCTGAG GATGGAATCC ATGAGCTATT CCCTGCACCT
    GATGGTGAAG AAGACACTGC AGAGCTGCAA GGCCTCAGAC CGGGTTCTGA GTACACAGTC
    AGTGTGGTTG CCTTGCACGA TGATATGGAG AGCCAGCCCC TGATTGGAAC CCAGTCCACA
    GCTATTCCTG CACCAACTGA CCTGAAGTTC ACTCAGGTCA CACCCACAAG CCTGAGCGCC
    CAGTGGACAC CACCCAATGT TCAGCTCACT GGATATCGAG TGCGGGTGAC CCCCAAGGAG
    AAGACCGGAC CAATGAAAGA AATCAACCTT GCTCCTGACA GCTCATCCGT GGTTGTATCA
    GGACTTATGG TGGCCACCAA ATATGAAGTG AGTGTCTATG CTCTTAAGGA CACTTTGACA
    AGCAGACCAG CTCAGGGAGT TGTCACCACT CTGGAGAATG TCAGCCCACC AAGAAGGGCT
    CGTGTGACAG ATGCTACTGA GACCACCATC ACCATTAGCT GGAGAACCAA GACTGAGACG
    ATCACTGGCT TCCAAGTTGA TGCCGTTCCA GCCAATGGCC AGACTCCAAT CCAGAGAACC
    ATCAAGCCAG ATGTCAGAAG CTACACCATC ACAGGTTTAC AACCAGGCAC TGACTACAAG
    ATCTACCTGT ACACCTTGAA TGACAATGCT CGGAGCTCCC CTGTGGTCAT CGACGCCTCC
    ACTGCCATTG ATGCACCATC CAACCTGCGT TTCCTGGCCA CCACACCCAA TTCCTTGCTG
    GTATCATGGC AGCCGCCACG TGCCAGGATT ACCGGCTACA TCATCAAGTA TGAGAAGCCT
    GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG CCCCGCCCTG GTGTCACAGA GGCTACTATT
    ACTGGCCTGG AACCGGGAAC CGAATATACA ATTTATGTCA TTGCCCTGAA GAATAATCAG
    AAGAGCGAGC CCCTGATTGG AAGGAAAAAG ACAGGACAAG AAGCTCTCTC TCAGACAACC
    ATCTCATGGG CCCCATTCCA GGACACTTCT GAGTACATCA TTTCATGTCA TCCTGTTGGC
    ACTGATGAAG AACCCTTACA GTTCAGGGTT CCTGGAACTT CTACCAGTGC CACTCTGACA
    GGCCTCACCA GAGGTGCCAC CTACAACGTC ATAGTGGAGG CACTGAAAGA CCAGCAGAGG
    CATAAGGTTC GGGAAGAGGT TGTTACCGTG GGCAACTCTG TCAACGAAGG CTTGAACCAA
    CCTACGGATG ACTCGTGCTT TGACCCCTAC ACAGTTTCCC ATTATGCCGT TGGAGATGAG
    TGGGAACGAA TGTCTGAATC AGGCTTTAAA CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT
    GGTCATTTCA GATGTGATTC ATCTAGATGG TGCCATGACA ATGGTGTGAA CTACAAGATT
    GGAGAGAAGT GGGACCGTCA GGGAGAAAAT GGCCAGATGA TGAGCTGCAC ATGTCTTGGG
    AACGGAAAAG GAGAATTCAA GTGTGACCCT CATGAGGCAA CGTGTTATGA TGATGGGAAG
    ACATACCACG TAGGAGAACA GTGGCAGAAG GAATATCTCG GTGCCATTTG CTCCTGCACA
    TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT GACAACTGCC GCAGACCTGG GGGTGAACCC
    AGTCCCGAAG GCACTACTGG CCAGTCCTAC AACCAGTATT CTCAGAGATA CCATCAGAGA
    ACAAACACTA ATGTTAATTG CCCAATTGAG TGCTTCATGC CTTTAGATGT ACAGGCTGAC
    AGAGAAGATT CCCGAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57835
    Clone ID Related Accession (Same CDS sequence) NM_001306131.1
    Accession Version NM_001306131.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6798bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Homo sapiens(human)
    Product fibronectin isoform 10 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA851446.1, BX640803.1, AJ535086.1, BX640875.1, CR749281.1, AI033037.1, U60068.1, BX640999.1, BQ005645.1 and CR749317.1. On Apr 15, 2015 this sequence version replaced XM_005246415.1. Transcript Variant: This variant (10) lacks two alternate in-frame exons in the central and 3' coding regions, and contains a segment with an alternate splice structure in the 3' coding region, compared to variant 1. Isoform 10 encoded by this variant has a distinct internal amino acid and is shorter than isoform 1. The encoded isoform (10) may undergo proteolytic processing similar to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.143010.1, CR749281.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA
    GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG
    CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC
    AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT
    GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC
    CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT
    CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA
    CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA
    GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG
    ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC
    CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC
    ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG
    TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT
    GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA
    GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC
    AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT
    GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA
    GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG
    AGTCAGCCTC TGGTTCAGAC TGCAGTAACC ACTATTCCTG CACCAACTGA CCTGAAGTTC
    ACTCAGGTCA CACCCACAAG CCTGAGCGCC CAGTGGACAC CACCCAATGT TCAGCTCACT
    GGATATCGAG TGCGGGTGAC CCCCAAGGAG AAGACCGGAC CAATGAAAGA AATCAACCTT
    GCTCCTGACA GCTCATCCGT GGTTGTATCA GGACTTATGG TGGCCACCAA ATATGAAGTG
    AGTGTCTATG CTCTTAAGGA CACTTTGACA AGCAGACCAG CTCAGGGAGT TGTCACCACT
    CTGGAGAATG TCAGCCCACC AAGAAGGGCT CGTGTGACAG ATGCTACTGA GACCACCATC
    ACCATTAGCT GGAGAACCAA GACTGAGACG ATCACTGGCT TCCAAGTTGA TGCCGTTCCA
    GCCAATGGCC AGACTCCAAT CCAGAGAACC ATCAAGCCAG ATGTCAGAAG CTACACCATC
    ACAGGTTTAC AACCAGGCAC TGACTACAAG ATCTACCTGT ACACCTTGAA TGACAATGCT
    CGGAGCTCCC CTGTGGTCAT CGACGCCTCC ACTGCCATTG ATGCACCATC CAACCTGCGT
    TTCCTGGCCA CCACACCCAA TTCCTTGCTG GTATCATGGC AGCCGCCACG TGCCAGGATT
    ACCGGCTACA TCATCAAGTA TGAGAAGCCT GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACAGA GGCTACTATT ACTGGCCTGG AACCGGGAAC CGAATATACA
    ATTTATGTCA TTGCCCTGAA GAATAATCAG AAGAGCGAGC CCCTGATTGG AAGGAAAAAG
    ACAGACGAGC TTCCCCAACT GGTAACCCTT CCACACCCCA ATCTTCATGG ACCAGAGATC
    TTGGATGTTC CTTCCACAGT TCAAAAGACC CCTTTCGTCA CCCACCCTGG GTATGACACT
    GGAAATGGTA TTCAGCTTCC TGGCACTTCT GGTCAGCAAC CCAGTGTTGG GCAACAAATG
    ATCTTTGAGG AACATGGTTT TAGGCGGACC ACACCGCCCA CAACGGCCAC CCCCATAAGG
    CATAGGCCAA GACCATACCC GCCGAATGTA GGACAAGAAG CTCTCTCTCA GACAACCATC
    TCATGGGCCC CATTCCAGGA CACTTCTGAG TACATCATTT CATGTCATCC TGTTGGCACT
    GATGAAGAAC CCTTACAGTT CAGGGTTCCT GGAACTTCTA CCAGTGCCAC TCTGACAGGC
    CTCACCAGAG GTGCCACCTA CAACATCATA GTGGAGGCAC TGAAAGACCA GCAGAGGCAT
    AAGGTTCGGG AAGAGGTTGT TACCGTGGGC AACTCTGTCA ACGAAGGCTT GAACCAACCT
    ACGGATGACT CGTGCTTTGA CCCCTACACA GTTTCCCATT ATGCCGTTGG AGATGAGTGG
    GAACGAATGT CTGAATCAGG CTTTAAACTG TTGTGCCAGT GCTTAGGCTT TGGAAGTGGT
    CATTTCAGAT GTGATTCATC TAGATGGTGC CATGACAATG GTGTGAACTA CAAGATTGGA
    GAGAAGTGGG ACCGTCAGGG AGAAAATGGC CAGATGATGA GCTGCACATG TCTTGGGAAC
    GGAAAAGGAG AATTCAAGTG TGACCCTCAT GAGGCAACGT GTTATGATGA TGGGAAGACA
    TACCACGTAG GAGAACAGTG GCAGAAGGAA TATCTCGGTG CCATTTGCTC CTGCACATGC
    TTTGGAGGCC AGCGGGGCTG GCGCTGTGAC AACTGCCGCA GACCTGGGGG TGAACCCAGT
    CCCGAAGGCA CTACTGGCCA GTCCTACAAC CAGTATTCTC AGAGATACCA TCAGAGAACA
    AACACTAATG TTAATTGCCC AATTGAGTGC TTCATGCCTT TAGATGTACA GGCTGACAGA
    GAAGATTCCC GAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293060.1
    CDS269..7066
    Translation

    Target ORF information:

    RefSeq Version NM_001306131.1
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001306131.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA
    GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG
    CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC
    AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT
    GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC
    CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT
    CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA
    CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA
    GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG
    ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC
    CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC
    ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG
    TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT
    GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA
    GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC
    AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT
    GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA
    GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG
    AGTCAGCCTC TGGTTCAGAC TGCAGTAACC ACTATTCCTG CACCAACTGA CCTGAAGTTC
    ACTCAGGTCA CACCCACAAG CCTGAGCGCC CAGTGGACAC CACCCAATGT TCAGCTCACT
    GGATATCGAG TGCGGGTGAC CCCCAAGGAG AAGACCGGAC CAATGAAAGA AATCAACCTT
    GCTCCTGACA GCTCATCCGT GGTTGTATCA GGACTTATGG TGGCCACCAA ATATGAAGTG
    AGTGTCTATG CTCTTAAGGA CACTTTGACA AGCAGACCAG CTCAGGGAGT TGTCACCACT
    CTGGAGAATG TCAGCCCACC AAGAAGGGCT CGTGTGACAG ATGCTACTGA GACCACCATC
    ACCATTAGCT GGAGAACCAA GACTGAGACG ATCACTGGCT TCCAAGTTGA TGCCGTTCCA
    GCCAATGGCC AGACTCCAAT CCAGAGAACC ATCAAGCCAG ATGTCAGAAG CTACACCATC
    ACAGGTTTAC AACCAGGCAC TGACTACAAG ATCTACCTGT ACACCTTGAA TGACAATGCT
    CGGAGCTCCC CTGTGGTCAT CGACGCCTCC ACTGCCATTG ATGCACCATC CAACCTGCGT
    TTCCTGGCCA CCACACCCAA TTCCTTGCTG GTATCATGGC AGCCGCCACG TGCCAGGATT
    ACCGGCTACA TCATCAAGTA TGAGAAGCCT GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACAGA GGCTACTATT ACTGGCCTGG AACCGGGAAC CGAATATACA
    ATTTATGTCA TTGCCCTGAA GAATAATCAG AAGAGCGAGC CCCTGATTGG AAGGAAAAAG
    ACAGACGAGC TTCCCCAACT GGTAACCCTT CCACACCCCA ATCTTCATGG ACCAGAGATC
    TTGGATGTTC CTTCCACAGT TCAAAAGACC CCTTTCGTCA CCCACCCTGG GTATGACACT
    GGAAATGGTA TTCAGCTTCC TGGCACTTCT GGTCAGCAAC CCAGTGTTGG GCAACAAATG
    ATCTTTGAGG AACATGGTTT TAGGCGGACC ACACCGCCCA CAACGGCCAC CCCCATAAGG
    CATAGGCCAA GACCATACCC GCCGAATGTA GGACAAGAAG CTCTCTCTCA GACAACCATC
    TCATGGGCCC CATTCCAGGA CACTTCTGAG TACATCATTT CATGTCATCC TGTTGGCACT
    GATGAAGAAC CCTTACAGTT CAGGGTTCCT GGAACTTCTA CCAGTGCCAC TCTGACAGGC
    CTCACCAGAG GTGCCACCTA CAACATCATA GTGGAGGCAC TGAAAGACCA GCAGAGGCAT
    AAGGTTCGGG AAGAGGTTGT TACCGTGGGC AACTCTGTCA ACGAAGGCTT GAACCAACCT
    ACGGATGACT CGTGCTTTGA CCCCTACACA GTTTCCCATT ATGCCGTTGG AGATGAGTGG
    GAACGAATGT CTGAATCAGG CTTTAAACTG TTGTGCCAGT GCTTAGGCTT TGGAAGTGGT
    CATTTCAGAT GTGATTCATC TAGATGGTGC CATGACAATG GTGTGAACTA CAAGATTGGA
    GAGAAGTGGG ACCGTCAGGG AGAAAATGGC CAGATGATGA GCTGCACATG TCTTGGGAAC
    GGAAAAGGAG AATTCAAGTG TGACCCTCAT GAGGCAACGT GTTATGATGA TGGGAAGACA
    TACCACGTAG GAGAACAGTG GCAGAAGGAA TATCTCGGTG CCATTTGCTC CTGCACATGC
    TTTGGAGGCC AGCGGGGCTG GCGCTGTGAC AACTGCCGCA GACCTGGGGG TGAACCCAGT
    CCCGAAGGCA CTACTGGCCA GTCCTACAAC CAGTATTCTC AGAGATACCA TCAGAGAACA
    AACACTAATG TTAATTGCCC AATTGAGTGC TTCATGCCTT TAGATGTACA GGCTGACAGA
    GAAGATTCCC GAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57836
    Clone ID Related Accession (Same CDS sequence) NM_001306129.1
    Accession Version NM_001306129.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7341bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Homo sapiens(human)
    Product fibronectin isoform 8 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA851446.1, BX640803.1, AJ535086.1, BX640875.1, CR749316.1, AI033037.1, BX537590.1, BC143754.1, CA422234.1 and CR749317.1. On Apr 15, 2015 this sequence version replaced XM_005246400.1. Transcript Variant: This variant (8) contains a segment with an alternate splice structure in the 3' coding region, compared to variant 1. Isoform 8 encoded by this variant is shorter than isoform 1. The encoded isoform (8) may undergo proteolytic processing similar to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX537590.1, SRR1660803.84543.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA
    ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT
    GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG
    CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG
    GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG
    TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC
    ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC
    CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC
    ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT
    CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC
    ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT
    GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG
    ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT
    ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA
    AAGACAGACG AGCTTCCCCA ACTGGTAACC CTTCCACACC CCAATCTTCA TGGACCAGAG
    ATCTTGGATG TTCCTTCCAC AGTTCAAAAG ACCCCTTTCG TCACCCACCC TGGGTATGAC
    ACTGGAAATG GTATTCAGCT TCCTGGCACT TCTGGTCAGC AACCCAGTGT TGGGCAACAA
    ATGATCTTTG AGGAACATGG TTTTAGGCGG ACCACACCGC CCACAACGGC CACCCCCATA
    AGGCATAGGC CAAGACCATA CCCGCCGAAT GTAGGACAAG AAGCTCTCTC TCAGACAACC
    ATCTCATGGG CCCCATTCCA GGACACTTCT GAGTACATCA TTTCATGTCA TCCTGTTGGC
    ACTGATGAAG AACCCTTACA GTTCAGGGTT CCTGGAACTT CTACCAGTGC CACTCTGACA
    GGCCTCACCA GAGGTGCCAC CTACAACATC ATAGTGGAGG CACTGAAAGA CCAGCAGAGG
    CATAAGGTTC GGGAAGAGGT TGTTACCGTG GGCAACTCTG TCAACGAAGG CTTGAACCAA
    CCTACGGATG ACTCGTGCTT TGACCCCTAC ACAGTTTCCC ATTATGCCGT TGGAGATGAG
    TGGGAACGAA TGTCTGAATC AGGCTTTAAA CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT
    GGTCATTTCA GATGTGATTC ATCTAGATGG TGCCATGACA ATGGTGTGAA CTACAAGATT
    GGAGAGAAGT GGGACCGTCA GGGAGAAAAT GGCCAGATGA TGAGCTGCAC ATGTCTTGGG
    AACGGAAAAG GAGAATTCAA GTGTGACCCT CATGAGGCAA CGTGTTATGA TGATGGGAAG
    ACATACCACG TAGGAGAACA GTGGCAGAAG GAATATCTCG GTGCCATTTG CTCCTGCACA
    TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT GACAACTGCC GCAGACCTGG GGGTGAACCC
    AGTCCCGAAG GCACTACTGG CCAGTCCTAC AACCAGTATT CTCAGAGATA CCATCAGAGA
    ACAAACACTA ATGTTAATTG CCCAATTGAG TGCTTCATGC CTTTAGATGT ACAGGCTGAC
    AGAGAAGATT CCCGAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293058.1
    CDS269..7609
    Translation

    Target ORF information:

    RefSeq Version NM_001306129.1
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001306129.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA
    ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT
    GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG
    CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG
    GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG
    TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC
    ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC
    CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC
    ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT
    CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC
    ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT
    GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG
    ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT
    ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA
    AAGACAGACG AGCTTCCCCA ACTGGTAACC CTTCCACACC CCAATCTTCA TGGACCAGAG
    ATCTTGGATG TTCCTTCCAC AGTTCAAAAG ACCCCTTTCG TCACCCACCC TGGGTATGAC
    ACTGGAAATG GTATTCAGCT TCCTGGCACT TCTGGTCAGC AACCCAGTGT TGGGCAACAA
    ATGATCTTTG AGGAACATGG TTTTAGGCGG ACCACACCGC CCACAACGGC CACCCCCATA
    AGGCATAGGC CAAGACCATA CCCGCCGAAT GTAGGACAAG AAGCTCTCTC TCAGACAACC
    ATCTCATGGG CCCCATTCCA GGACACTTCT GAGTACATCA TTTCATGTCA TCCTGTTGGC
    ACTGATGAAG AACCCTTACA GTTCAGGGTT CCTGGAACTT CTACCAGTGC CACTCTGACA
    GGCCTCACCA GAGGTGCCAC CTACAACATC ATAGTGGAGG CACTGAAAGA CCAGCAGAGG
    CATAAGGTTC GGGAAGAGGT TGTTACCGTG GGCAACTCTG TCAACGAAGG CTTGAACCAA
    CCTACGGATG ACTCGTGCTT TGACCCCTAC ACAGTTTCCC ATTATGCCGT TGGAGATGAG
    TGGGAACGAA TGTCTGAATC AGGCTTTAAA CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT
    GGTCATTTCA GATGTGATTC ATCTAGATGG TGCCATGACA ATGGTGTGAA CTACAAGATT
    GGAGAGAAGT GGGACCGTCA GGGAGAAAAT GGCCAGATGA TGAGCTGCAC ATGTCTTGGG
    AACGGAAAAG GAGAATTCAA GTGTGACCCT CATGAGGCAA CGTGTTATGA TGATGGGAAG
    ACATACCACG TAGGAGAACA GTGGCAGAAG GAATATCTCG GTGCCATTTG CTCCTGCACA
    TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT GACAACTGCC GCAGACCTGG GGGTGAACCC
    AGTCCCGAAG GCACTACTGG CCAGTCCTAC AACCAGTATT CTCAGAGATA CCATCAGAGA
    ACAAACACTA ATGTTAATTG CCCAATTGAG TGCTTCATGC CTTTAGATGT ACAGGCTGAC
    AGAGAAGATT CCCGAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57837
    Clone ID Related Accession (Same CDS sequence) NM_001306130.1
    Accession Version NM_001306130.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6804bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Homo sapiens(human)
    Product fibronectin isoform 9 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA851446.1, BX640803.1, AJ535086.1, BX640875.1, CR749316.1, AI033037.1, BC143754.1, CA422234.1 and CR749317.1. On Apr 15, 2015 this sequence version replaced XM_005246413.1. Transcript Variant: This variant (9) lacks an alternate in-frame exon and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. Isoform 9 encoded by this variant has a distinct internal amino acid and is shorter than isoform 1. The encoded isoform (9) may undergo proteolytic processing similar to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR749316.1, SRR1660803.216975.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC
    GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG
    GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA
    TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG
    GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG
    ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA
    ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC
    AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG
    CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG
    CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT
    ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT
    CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGGAC AAGAAGCTCT CTCTCAGACA
    ACCATCTCAT GGGCCCCATT CCAGGACACT TCTGAGTACA TCATTTCATG TCATCCTGTT
    GGCACTGATG AAGAACCCTT ACAGTTCAGG GTTCCTGGAA CTTCTACCAG TGCCACTCTG
    ACAGGCCTCA CCAGAGGTGC CACCTACAAC ATCATAGTGG AGGCACTGAA AGACCAGCAG
    AGGCATAAGG TTCGGGAAGA GGTTGTTACC GTGGGCAACT CTGTCAACGA AGGCTTGAAC
    CAACCTACGG ATGACTCGTG CTTTGACCCC TACACAGTTT CCCATTATGC CGTTGGAGAT
    GAGTGGGAAC GAATGTCTGA ATCAGGCTTT AAACTGTTGT GCCAGTGCTT AGGCTTTGGA
    AGTGGTCATT TCAGATGTGA TTCATCTAGA TGGTGCCATG ACAATGGTGT GAACTACAAG
    ATTGGAGAGA AGTGGGACCG TCAGGGAGAA AATGGCCAGA TGATGAGCTG CACATGTCTT
    GGGAACGGAA AAGGAGAATT CAAGTGTGAC CCTCATGAGG CAACGTGTTA TGATGATGGG
    AAGACATACC ACGTAGGAGA ACAGTGGCAG AAGGAATATC TCGGTGCCAT TTGCTCCTGC
    ACATGCTTTG GAGGCCAGCG GGGCTGGCGC TGTGACAACT GCCGCAGACC TGGGGGTGAA
    CCCAGTCCCG AAGGCACTAC TGGCCAGTCC TACAACCAGT ATTCTCAGAG ATACCATCAG
    AGAACAAACA CTAATGTTAA TTGCCCAATT GAGTGCTTCA TGCCTTTAGA TGTACAGGCT
    GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293059.1
    CDS269..7072
    Translation

    Target ORF information:

    RefSeq Version NM_001306130.1
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001306130.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCACTATTC CTGCACCAAC TGACCTGAAG TTCACTCAGG TCACACCCAC AAGCCTGAGC
    GCCCAGTGGA CACCACCCAA TGTTCAGCTC ACTGGATATC GAGTGCGGGT GACCCCCAAG
    GAGAAGACCG GACCAATGAA AGAAATCAAC CTTGCTCCTG ACAGCTCATC CGTGGTTGTA
    TCAGGACTTA TGGTGGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CAGCTCAGGG AGTTGTCACC ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG
    GCTCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC CAAGACTGAG
    ACGATCACTG GCTTCCAAGT TGATGCCGTT CCAGCCAATG GCCAGACTCC AATCCAGAGA
    ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TACAACCAGG CACTGACTAC
    AAGATCTACC TGTACACCTT GAATGACAAT GCTCGGAGCT CCCCTGTGGT CATCGACGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAATTCCTTG
    CTGGTATCAT GGCAGCCGCC ACGTGCCAGG ATTACCGGCT ACATCATCAA GTATGAGAAG
    CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAGGCTACT
    ATTACTGGCC TGGAACCGGG AACCGAATAT ACAATTTATG TCATTGCCCT GAAGAATAAT
    CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA AAGACAGGAC AAGAAGCTCT CTCTCAGACA
    ACCATCTCAT GGGCCCCATT CCAGGACACT TCTGAGTACA TCATTTCATG TCATCCTGTT
    GGCACTGATG AAGAACCCTT ACAGTTCAGG GTTCCTGGAA CTTCTACCAG TGCCACTCTG
    ACAGGCCTCA CCAGAGGTGC CACCTACAAC ATCATAGTGG AGGCACTGAA AGACCAGCAG
    AGGCATAAGG TTCGGGAAGA GGTTGTTACC GTGGGCAACT CTGTCAACGA AGGCTTGAAC
    CAACCTACGG ATGACTCGTG CTTTGACCCC TACACAGTTT CCCATTATGC CGTTGGAGAT
    GAGTGGGAAC GAATGTCTGA ATCAGGCTTT AAACTGTTGT GCCAGTGCTT AGGCTTTGGA
    AGTGGTCATT TCAGATGTGA TTCATCTAGA TGGTGCCATG ACAATGGTGT GAACTACAAG
    ATTGGAGAGA AGTGGGACCG TCAGGGAGAA AATGGCCAGA TGATGAGCTG CACATGTCTT
    GGGAACGGAA AAGGAGAATT CAAGTGTGAC CCTCATGAGG CAACGTGTTA TGATGATGGG
    AAGACATACC ACGTAGGAGA ACAGTGGCAG AAGGAATATC TCGGTGCCAT TTGCTCCTGC
    ACATGCTTTG GAGGCCAGCG GGGCTGGCGC TGTGACAACT GCCGCAGACC TGGGGGTGAA
    CCCAGTCCCG AAGGCACTAC TGGCCAGTCC TACAACCAGT ATTCTCAGAG ATACCATCAG
    AGAACAAACA CTAATGTTAA TTGCCCAATT GAGTGCTTCA TGCCTTTAGA TGTACAGGCT
    GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57838
    Clone ID Related Accession (Same CDS sequence) NM_001306132.1
    Accession Version NM_001306132.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6723bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Homo sapiens(human)
    Product fibronectin isoform 11 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA851446.1, BX640803.1, AJ535086.1, BX640875.1, BC143754.1, AI033037.1, U60068.1, CA422234.1 and CR749317.1. On Apr 15, 2015 this sequence version replaced XM_005246417.1. Transcript Variant: This variant (11) lacks two alternate in-frame exons in the central and 3' coding regions, and contains a segment with an alternate splice structure in the 3' coding region, compared to variant 1. Isoform 11 encoded by this variant has a distinct internal amino acid and is shorter than isoform 1. The encoded isoform (11) may undergo proteolytic processing similar to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR749317.1, SRR1803611.83883.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA
    GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG
    CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC
    AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT
    GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC
    CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT
    CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA
    CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA
    GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG
    ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC
    CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC
    ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG
    TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT
    GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA
    GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC
    AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT
    GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA
    GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG
    AGTCAGCCTC TGGTTCAGAC TGCAGTAACC ACTATTCCTG CACCAACTGA CCTGAAGTTC
    ACTCAGGTCA CACCCACAAG CCTGAGCGCC CAGTGGACAC CACCCAATGT TCAGCTCACT
    GGATATCGAG TGCGGGTGAC CCCCAAGGAG AAGACCGGAC CAATGAAAGA AATCAACCTT
    GCTCCTGACA GCTCATCCGT GGTTGTATCA GGACTTATGG TGGCCACCAA ATATGAAGTG
    AGTGTCTATG CTCTTAAGGA CACTTTGACA AGCAGACCAG CTCAGGGAGT TGTCACCACT
    CTGGAGAATG TCAGCCCACC AAGAAGGGCT CGTGTGACAG ATGCTACTGA GACCACCATC
    ACCATTAGCT GGAGAACCAA GACTGAGACG ATCACTGGCT TCCAAGTTGA TGCCGTTCCA
    GCCAATGGCC AGACTCCAAT CCAGAGAACC ATCAAGCCAG ATGTCAGAAG CTACACCATC
    ACAGGTTTAC AACCAGGCAC TGACTACAAG ATCTACCTGT ACACCTTGAA TGACAATGCT
    CGGAGCTCCC CTGTGGTCAT CGACGCCTCC ACTGCCATTG ATGCACCATC CAACCTGCGT
    TTCCTGGCCA CCACACCCAA TTCCTTGCTG GTATCATGGC AGCCGCCACG TGCCAGGATT
    ACCGGCTACA TCATCAAGTA TGAGAAGCCT GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACAGA GGCTACTATT ACTGGCCTGG AACCGGGAAC CGAATATACA
    ATTTATGTCA TTGCCCTGAA GAATAATCAG AAGAGCGAGC CCCTGATTGG AAGGAAAAAG
    ACAGTTCAAA AGACCCCTTT CGTCACCCAC CCTGGGTATG ACACTGGAAA TGGTATTCAG
    CTTCCTGGCA CTTCTGGTCA GCAACCCAGT GTTGGGCAAC AAATGATCTT TGAGGAACAT
    GGTTTTAGGC GGACCACACC GCCCACAACG GCCACCCCCA TAAGGCATAG GCCAAGACCA
    TACCCGCCGA ATGTAGGACA AGAAGCTCTC TCTCAGACAA CCATCTCATG GGCCCCATTC
    CAGGACACTT CTGAGTACAT CATTTCATGT CATCCTGTTG GCACTGATGA AGAACCCTTA
    CAGTTCAGGG TTCCTGGAAC TTCTACCAGT GCCACTCTGA CAGGCCTCAC CAGAGGTGCC
    ACCTACAACA TCATAGTGGA GGCACTGAAA GACCAGCAGA GGCATAAGGT TCGGGAAGAG
    GTTGTTACCG TGGGCAACTC TGTCAACGAA GGCTTGAACC AACCTACGGA TGACTCGTGC
    TTTGACCCCT ACACAGTTTC CCATTATGCC GTTGGAGATG AGTGGGAACG AATGTCTGAA
    TCAGGCTTTA AACTGTTGTG CCAGTGCTTA GGCTTTGGAA GTGGTCATTT CAGATGTGAT
    TCATCTAGAT GGTGCCATGA CAATGGTGTG AACTACAAGA TTGGAGAGAA GTGGGACCGT
    CAGGGAGAAA ATGGCCAGAT GATGAGCTGC ACATGTCTTG GGAACGGAAA AGGAGAATTC
    AAGTGTGACC CTCATGAGGC AACGTGTTAT GATGATGGGA AGACATACCA CGTAGGAGAA
    CAGTGGCAGA AGGAATATCT CGGTGCCATT TGCTCCTGCA CATGCTTTGG AGGCCAGCGG
    GGCTGGCGCT GTGACAACTG CCGCAGACCT GGGGGTGAAC CCAGTCCCGA AGGCACTACT
    GGCCAGTCCT ACAACCAGTA TTCTCAGAGA TACCATCAGA GAACAAACAC TAATGTTAAT
    TGCCCAATTG AGTGCTTCAT GCCTTTAGAT GTACAGGCTG ACAGAGAAGA TTCCCGAGAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293061.1
    CDS269..6991
    Translation

    Target ORF information:

    RefSeq Version NM_001306132.1
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant 11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001306132.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACCC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGCTGT TCCTCCTCCC ACTGACCTGC GATTCACCAA CATTGGTCCA
    GACACCATGC GTGTCACCTG GGCTCCACCC CCATCCATTG ATTTAACCAA CTTCCTGGTG
    CGTTACTCAC CTGTGAAAAA TGAGGAAGAT GTTGCAGAGT TGTCAATTTC TCCTTCAGAC
    AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGTACAGAAT ATGTAGTGAG TGTCTCCAGT
    GTCTACGAAC AACATGAGAG CACACCTCTT AGAGGAAGAC AGAAAACAGG TCTTGATTCC
    CCAACTGGCA TTGACTTTTC TGATATTACT GCCAACTCTT TTACTGTGCA CTGGATTGCT
    CCTCGAGCCA CCATCACTGG CTACAGGATC CGCCATCATC CCGAGCACTT CAGTGGGAGA
    CCTCGAGAAG ATCGGGTGCC CCACTCTCGG AATTCCATCA CCCTCACCAA CCTCACTCCA
    GGCACAGAGT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCCTTATTG
    ATTGGCCAAC AATCAACAGT TTCTGATGTT CCGAGGGACC TGGAAGTTGT TGCTGCGACC
    CCCACCAGCC TACTGATCAG CTGGGATGCT CCTGCTGTCA CAGTGAGATA TTACAGGATC
    ACTTACGGAG AGACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGGAGCAAG
    TCTACAGCTA CCATCAGCGG CCTTAAACCT GGAGTTGATT ATACCATCAC TGTGTATGCT
    GTCACTGGCC GTGGAGACAG CCCCGCAAGC AGCAAGCCAA TTTCCATTAA TTACCGAACA
    GAAATTGACA AACCATCCCA GATGCAAGTG ACCGATGTTC AGGACAACAG CATTAGTGTC
    AAGTGGCTGC CTTCAAGTTC CCCTGTTACT GGTTACAGAG TAACCACCAC TCCCAAAAAT
    GGACCAGGAC CAACAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACTATTGAA
    GGCTTGCAGC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCC AAGCGGAGAG
    AGTCAGCCTC TGGTTCAGAC TGCAGTAACC ACTATTCCTG CACCAACTGA CCTGAAGTTC
    ACTCAGGTCA CACCCACAAG CCTGAGCGCC CAGTGGACAC CACCCAATGT TCAGCTCACT
    GGATATCGAG TGCGGGTGAC CCCCAAGGAG AAGACCGGAC CAATGAAAGA AATCAACCTT
    GCTCCTGACA GCTCATCCGT GGTTGTATCA GGACTTATGG TGGCCACCAA ATATGAAGTG
    AGTGTCTATG CTCTTAAGGA CACTTTGACA AGCAGACCAG CTCAGGGAGT TGTCACCACT
    CTGGAGAATG TCAGCCCACC AAGAAGGGCT CGTGTGACAG ATGCTACTGA GACCACCATC
    ACCATTAGCT GGAGAACCAA GACTGAGACG ATCACTGGCT TCCAAGTTGA TGCCGTTCCA
    GCCAATGGCC AGACTCCAAT CCAGAGAACC ATCAAGCCAG ATGTCAGAAG CTACACCATC
    ACAGGTTTAC AACCAGGCAC TGACTACAAG ATCTACCTGT ACACCTTGAA TGACAATGCT
    CGGAGCTCCC CTGTGGTCAT CGACGCCTCC ACTGCCATTG ATGCACCATC CAACCTGCGT
    TTCCTGGCCA CCACACCCAA TTCCTTGCTG GTATCATGGC AGCCGCCACG TGCCAGGATT
    ACCGGCTACA TCATCAAGTA TGAGAAGCCT GGGTCTCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACAGA GGCTACTATT ACTGGCCTGG AACCGGGAAC CGAATATACA
    ATTTATGTCA TTGCCCTGAA GAATAATCAG AAGAGCGAGC CCCTGATTGG AAGGAAAAAG
    ACAGTTCAAA AGACCCCTTT CGTCACCCAC CCTGGGTATG ACACTGGAAA TGGTATTCAG
    CTTCCTGGCA CTTCTGGTCA GCAACCCAGT GTTGGGCAAC AAATGATCTT TGAGGAACAT
    GGTTTTAGGC GGACCACACC GCCCACAACG GCCACCCCCA TAAGGCATAG GCCAAGACCA
    TACCCGCCGA ATGTAGGACA AGAAGCTCTC TCTCAGACAA CCATCTCATG GGCCCCATTC
    CAGGACACTT CTGAGTACAT CATTTCATGT CATCCTGTTG GCACTGATGA AGAACCCTTA
    CAGTTCAGGG TTCCTGGAAC TTCTACCAGT GCCACTCTGA CAGGCCTCAC CAGAGGTGCC
    ACCTACAACA TCATAGTGGA GGCACTGAAA GACCAGCAGA GGCATAAGGT TCGGGAAGAG
    GTTGTTACCG TGGGCAACTC TGTCAACGAA GGCTTGAACC AACCTACGGA TGACTCGTGC
    TTTGACCCCT ACACAGTTTC CCATTATGCC GTTGGAGATG AGTGGGAACG AATGTCTGAA
    TCAGGCTTTA AACTGTTGTG CCAGTGCTTA GGCTTTGGAA GTGGTCATTT CAGATGTGAT
    TCATCTAGAT GGTGCCATGA CAATGGTGTG AACTACAAGA TTGGAGAGAA GTGGGACCGT
    CAGGGAGAAA ATGGCCAGAT GATGAGCTGC ACATGTCTTG GGAACGGAAA AGGAGAATTC
    AAGTGTGACC CTCATGAGGC AACGTGTTAT GATGATGGGA AGACATACCA CGTAGGAGAA
    CAGTGGCAGA AGGAATATCT CGGTGCCATT TGCTCCTGCA CATGCTTTGG AGGCCAGCGG
    GGCTGGCGCT GTGACAACTG CCGCAGACCT GGGGGTGAAC CCAGTCCCGA AGGCACTACT
    GGCCAGTCCT ACAACCAGTA TTCTCAGAGA TACCATCAGA GAACAAACAC TAATGTTAAT
    TGCCCAATTG AGTGCTTCAT GCCTTTAGAT GTACAGGCTG ACAGAGAAGA TTCCCGAGAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36012
    Clone ID Related Accession (Same CDS sequence) XM_005246397.1
    Accession Version XM_005246397.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7452bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product fibronectin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA
    ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT
    GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG
    CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG
    GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG
    TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC
    ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC
    CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC
    ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT
    CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC
    ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT
    GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG
    ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT
    ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA
    AAGACAGACG AGCTTCCCCA ACTGGTAACC CTTCCACACC CCAATCTTCA TGGACCAGAG
    ATCTTGGATG TTCCTTCCAC AGTTCAAAAG ACCCCTTTCG TCACCCACCC TGGGTATGAC
    ACTGGAAATG GTATTCAGCT TCCTGGCACT TCTGGTCAGC AACCCAGTGT TGGGCAACAA
    ATGATCTTTG AGGAACATGG TTTTAGGCGG ACCACACCGC CCACAACGGC CACCCCCATA
    AGGCATAGGC CAAGACCATA CCCGCCGAAT GTAGGTGAGG AAATCCAAAT TGGTCACATC
    CCCAGGGAAG ATGTAGACTA TCACCTGTAC CCACACGGTC CGGGACTCAA TCCAAATGCC
    TCTACAGGAC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGGCCCCATT CCAGGACACT
    TCTGAGTACA TCATTTCATG TCATCCTGTT GGCACTGATG AAGAACCCTT ACAGTTCAGG
    GTTCCTGGAA CTTCTACCAG TGCCACTCTG ACAGGCCTCA CCAGAGGTGC CACCTACAAC
    GTCATAGTGG AGGCACTGAA AGACCAGCAG AGGCATAAGG TTCGGGAAGA GGTTGTTACC
    GTGGGCAACT CTGTCAACGA AGGCTTGAAC CAACCTACGG ATGACTCGTG CTTTGACCCC
    TACACAGTTT CCCATTATGC CGTTGGAGAT GAGTGGGAAC GAATGTCTGA ATCAGGCTTT
    AAACTGTTGT GCCAGTGCTT AGGCTTTGGA AGTGGTCATT TCAGATGTGA TTCATCTAGA
    TGGTGCCATG ACAATGGTGT GAACTACAAG ATTGGAGAGA AGTGGGACCG TCAGGGAGAA
    AATGGCCAGA TGATGAGCTG CACATGTCTT GGGAACGGAA AAGGAGAATT CAAGTGTGAC
    CCTCATGAGG CAACGTGTTA TGATGATGGG AAGACATACC ACGTAGGAGA ACAGTGGCAG
    AAGGAATATC TCGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC
    TGTGACAACT GCCGCAGACC TGGGGGTGAA CCCAGTCCCG AAGGCACTAC TGGCCAGTCC
    TACAACCAGT ATTCTCAGAG ATACCATCAG AGAACAAACA CTGTAAGTGC ATTAGCAGCA
    CAAGTGTGTT CCCTCATACT AGACAGTCTC TTTCTACAGG TATCTTTCTT CAGAATGAAC
    CAAGTGTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005246454.1
    CDS366..7817
    Translation

    Target ORF information:

    RefSeq Version XM_005246397.1
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005246397.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA
    ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT
    GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG
    CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG
    GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG
    TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC
    ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC
    CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC
    ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT
    CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC
    ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT
    GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG
    ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT
    ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA
    AAGACAGACG AGCTTCCCCA ACTGGTAACC CTTCCACACC CCAATCTTCA TGGACCAGAG
    ATCTTGGATG TTCCTTCCAC AGTTCAAAAG ACCCCTTTCG TCACCCACCC TGGGTATGAC
    ACTGGAAATG GTATTCAGCT TCCTGGCACT TCTGGTCAGC AACCCAGTGT TGGGCAACAA
    ATGATCTTTG AGGAACATGG TTTTAGGCGG ACCACACCGC CCACAACGGC CACCCCCATA
    AGGCATAGGC CAAGACCATA CCCGCCGAAT GTAGGTGAGG AAATCCAAAT TGGTCACATC
    CCCAGGGAAG ATGTAGACTA TCACCTGTAC CCACACGGTC CGGGACTCAA TCCAAATGCC
    TCTACAGGAC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGGCCCCATT CCAGGACACT
    TCTGAGTACA TCATTTCATG TCATCCTGTT GGCACTGATG AAGAACCCTT ACAGTTCAGG
    GTTCCTGGAA CTTCTACCAG TGCCACTCTG ACAGGCCTCA CCAGAGGTGC CACCTACAAC
    GTCATAGTGG AGGCACTGAA AGACCAGCAG AGGCATAAGG TTCGGGAAGA GGTTGTTACC
    GTGGGCAACT CTGTCAACGA AGGCTTGAAC CAACCTACGG ATGACTCGTG CTTTGACCCC
    TACACAGTTT CCCATTATGC CGTTGGAGAT GAGTGGGAAC GAATGTCTGA ATCAGGCTTT
    AAACTGTTGT GCCAGTGCTT AGGCTTTGGA AGTGGTCATT TCAGATGTGA TTCATCTAGA
    TGGTGCCATG ACAATGGTGT GAACTACAAG ATTGGAGAGA AGTGGGACCG TCAGGGAGAA
    AATGGCCAGA TGATGAGCTG CACATGTCTT GGGAACGGAA AAGGAGAATT CAAGTGTGAC
    CCTCATGAGG CAACGTGTTA TGATGATGGG AAGACATACC ACGTAGGAGA ACAGTGGCAG
    AAGGAATATC TCGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC
    TGTGACAACT GCCGCAGACC TGGGGGTGAA CCCAGTCCCG AAGGCACTAC TGGCCAGTCC
    TACAACCAGT ATTCTCAGAG ATACCATCAG AGAACAAACA CTGTAAGTGC ATTAGCAGCA
    CAAGTGTGTT CCCTCATACT AGACAGTCTC TTTCTACAGG TATCTTTCTT CAGAATGAAC
    CAAGTGTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36013
    Clone ID Related Accession (Same CDS sequence) XM_005246398.1
    Accession Version XM_005246398.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7377bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product fibronectin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA
    ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT
    GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG
    CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG
    GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG
    TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC
    ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC
    CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC
    ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT
    CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC
    ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT
    GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG
    ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT
    ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA
    AAGACAGTTC AAAAGACCCC TTTCGTCACC CACCCTGGGT ATGACACTGG AAATGGTATT
    CAGCTTCCTG GCACTTCTGG TCAGCAACCC AGTGTTGGGC AACAAATGAT CTTTGAGGAA
    CATGGTTTTA GGCGGACCAC ACCGCCCACA ACGGCCACCC CCATAAGGCA TAGGCCAAGA
    CCATACCCGC CGAATGTAGG TGAGGAAATC CAAATTGGTC ACATCCCCAG GGAAGATGTA
    GACTATCACC TGTACCCACA CGGTCCGGGA CTCAATCCAA ATGCCTCTAC AGGACAAGAA
    GCTCTCTCTC AGACAACCAT CTCATGGGCC CCATTCCAGG ACACTTCTGA GTACATCATT
    TCATGTCATC CTGTTGGCAC TGATGAAGAA CCCTTACAGT TCAGGGTTCC TGGAACTTCT
    ACCAGTGCCA CTCTGACAGG CCTCACCAGA GGTGCCACCT ACAACGTCAT AGTGGAGGCA
    CTGAAAGACC AGCAGAGGCA TAAGGTTCGG GAAGAGGTTG TTACCGTGGG CAACTCTGTC
    AACGAAGGCT TGAACCAACC TACGGATGAC TCGTGCTTTG ACCCCTACAC AGTTTCCCAT
    TATGCCGTTG GAGATGAGTG GGAACGAATG TCTGAATCAG GCTTTAAACT GTTGTGCCAG
    TGCTTAGGCT TTGGAAGTGG TCATTTCAGA TGTGATTCAT CTAGATGGTG CCATGACAAT
    GGTGTGAACT ACAAGATTGG AGAGAAGTGG GACCGTCAGG GAGAAAATGG CCAGATGATG
    AGCTGCACAT GTCTTGGGAA CGGAAAAGGA GAATTCAAGT GTGACCCTCA TGAGGCAACG
    TGTTATGATG ATGGGAAGAC ATACCACGTA GGAGAACAGT GGCAGAAGGA ATATCTCGGT
    GCCATTTGCT CCTGCACATG CTTTGGAGGC CAGCGGGGCT GGCGCTGTGA CAACTGCCGC
    AGACCTGGGG GTGAACCCAG TCCCGAAGGC ACTACTGGCC AGTCCTACAA CCAGTATTCT
    CAGAGATACC ATCAGAGAAC AAACACTGTA AGTGCATTAG CAGCACAAGT GTGTTCCCTC
    ATACTAGACA GTCTCTTTCT ACAGGTATCT TTCTTCAGAA TGAACCAAGT GTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005246455.1
    CDS366..7742
    Translation

    Target ORF information:

    RefSeq Version XM_005246398.1
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005246398.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA
    ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT
    GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG
    CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG
    GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG
    TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC
    ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC
    CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC
    ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT
    CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC
    ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT
    GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG
    ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT
    ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA
    AAGACAGTTC AAAAGACCCC TTTCGTCACC CACCCTGGGT ATGACACTGG AAATGGTATT
    CAGCTTCCTG GCACTTCTGG TCAGCAACCC AGTGTTGGGC AACAAATGAT CTTTGAGGAA
    CATGGTTTTA GGCGGACCAC ACCGCCCACA ACGGCCACCC CCATAAGGCA TAGGCCAAGA
    CCATACCCGC CGAATGTAGG TGAGGAAATC CAAATTGGTC ACATCCCCAG GGAAGATGTA
    GACTATCACC TGTACCCACA CGGTCCGGGA CTCAATCCAA ATGCCTCTAC AGGACAAGAA
    GCTCTCTCTC AGACAACCAT CTCATGGGCC CCATTCCAGG ACACTTCTGA GTACATCATT
    TCATGTCATC CTGTTGGCAC TGATGAAGAA CCCTTACAGT TCAGGGTTCC TGGAACTTCT
    ACCAGTGCCA CTCTGACAGG CCTCACCAGA GGTGCCACCT ACAACGTCAT AGTGGAGGCA
    CTGAAAGACC AGCAGAGGCA TAAGGTTCGG GAAGAGGTTG TTACCGTGGG CAACTCTGTC
    AACGAAGGCT TGAACCAACC TACGGATGAC TCGTGCTTTG ACCCCTACAC AGTTTCCCAT
    TATGCCGTTG GAGATGAGTG GGAACGAATG TCTGAATCAG GCTTTAAACT GTTGTGCCAG
    TGCTTAGGCT TTGGAAGTGG TCATTTCAGA TGTGATTCAT CTAGATGGTG CCATGACAAT
    GGTGTGAACT ACAAGATTGG AGAGAAGTGG GACCGTCAGG GAGAAAATGG CCAGATGATG
    AGCTGCACAT GTCTTGGGAA CGGAAAAGGA GAATTCAAGT GTGACCCTCA TGAGGCAACG
    TGTTATGATG ATGGGAAGAC ATACCACGTA GGAGAACAGT GGCAGAAGGA ATATCTCGGT
    GCCATTTGCT CCTGCACATG CTTTGGAGGC CAGCGGGGCT GGCGCTGTGA CAACTGCCGC
    AGACCTGGGG GTGAACCCAG TCCCGAAGGC ACTACTGGCC AGTCCTACAA CCAGTATTCT
    CAGAGATACC ATCAGAGAAC AAACACTGTA AGTGCATTAG CAGCACAAGT GTGTTCCCTC
    ATACTAGACA GTCTCTTTCT ACAGGTATCT TTCTTCAGAA TGAACCAAGT GTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36014
    Clone ID Related Accession (Same CDS sequence) XM_005246399.1
    Accession Version XM_005246399.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product fibronectin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC AGGACACAAG GACATCCTAT
    AGAATTGGAG ACACCTGGAG CAAGAAGGAT AATCGAGGAA ACCTGCTCCA GTGCATCTGC
    ACAGGCAACG GCCGAGGAGA GTGGAAGTGT GAGAGGCACA CCTCTGTGCA GACCACATCG
    AGCGGATCTG GCCCCTTCAC CGATGTTCGT GCAGCTGTTT ACCAACCGCA GCCTCACCCC
    CAGCCTCCTC CCTATGGCCA CTGTGTCACA GACAGTGGTG TGGTCTACTC TGTGGGGATG
    CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CGTGCCTGGG CAACGGAGTC
    AGCTGCCAAG AGACAGCTGT AACCCAGACT TACGGTGGCA ACTCAAATGG AGAGCCATGT
    GTCTTACCAT TCACCTACAA TGGCAGGACG TTCTACTCCT GCACCACAGA AGGGCGACAG
    GACGGACATC TTTGGTGCAG CACAACTTCG AATTATGAGC AGGACCAGAA ATACTCTTTC
    TGCACAGACC ACACTGTTTT GGTTCAGACT CGAGGAGGAA ATTCCAATGG TGCCTTGTGC
    CACTTCCCCT TCCTATACAA CAACCACAAT TACACTGATT GCACTTCTGA GGGCAGAAGA
    GACAACATGA AGTGGTGTGG GACCACACAG AACTATGATG CCGACCAGAA GTTTGGGTTC
    TGCCCCATGG CTGCCCACGA GGAAATCTGC ACAACCAATG AAGGGGTCAT GTACCGCATT
    GGAGATCAGT GGGATAAGCA GCATGACATG GGTCACATGA TGAGGTGCAC GTGTGTTGGG
    AATGGTCGTG GGGAATGGAC ATGCATTGCC TACTCGCAGC TTCGAGATCA GTGCATTGTT
    GATGACATCA CTTACAATGT GAACGACACA TTCCACAAGC GTCATGAAGA GGGGCACATG
    CTGAACTGTA CATGCTTCGG TCAGGGTCGG GGCAGGTGGA AGTGTGATCC CGTCGACCAA
    TGCCAGGATT CAGAGACTGG GACGTTTTAT CAAATTGGAG ATTCATGGGA GAAGTATGTG
    CATGGTGTCA GATACCAGTG CTACTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA
    CCTTTACAGA CCTATCCAAG CTCAAGTGGT CCTGTCGAAG TATTTATCAC TGAGACTCCG
    AGTCAGCCCA ACTCCCACCC CATCCAGTGG AATGCACCAC AGCCATCTCA CATTTCCAAG
    TACATTCTCA GGTGGAGACC TAAAAATTCT GTAGGCCGTT GGAAGGAAGC TACCATACCA
    GGCCACTTAA ACTCCTACAC CATCAAAGGC CTGAAGCCTG GTGTGGTATA CGAGGGCCAG
    CTCATCAGCA TCCAGCAGTA CGGCCACCAA GAAGTGACTC GCTTTGACTT CACCACCACC
    AGCACCAGCA CACCTGTGAC CAGCAACACC GTGACAGGAG AGACGACTCC CTTTTCTCCT
    CTTGTGGCCA CTTCTGAATC TGTGACCGAA ATCACAGCCA GTAGCTTTGT GGTCTCCTGG
    GTCTCAGCTT CCGACACCGT GTCGGGATTC CGGGTGGAAT ATGAGCTGAG TGAGGAGGGA
    GATGAGCCAC AGTACCTGGA TCTTCCAAGC ACAGCCACTT CTGTGAACAT CCCTGACCTG
    CTTCCTGGCC GAAAATACAT TGTAAATGTC TATCAGATAT CTGAGGATGG GGAGCAGAGT
    TTGATCCTGT CTACTTCACA AACAACAGCG CCTGATGCCC CTCCTGACAC GACTGTGGAC
    CAAGTTGATG ACACCTCAAT TGTTGTTCGC TGGAGCAGAC CCCAGGCTCC CATCACAGGG
    TACAGAATAG TCTATTCGCC ATCAGTAGAA GGTAGCAGCA CAGAACTCAA CCTTCCTGAA
    ACTGCAAACT CCGTCACCCT CAGTGACTTG CAACCTGGTG TTCAGTATAA CATCACTATC
    TATGCTGTGG AAGAAAATCA AGAAAGTACA CCTGTTGTCA TTCAACAAGA AACCACTGGC
    ACCCCACGCT CAGATACAGT GCCCTCTCCC AGGGACCTGC AGTTTGTGGA AGTGACAGAC
    GTGAAGGTCA CCATCATGTG GACACCGCCT GAGAGTGCAG TGACCGGCTA CCGTGTGGAT
    GTGATCCCCG TCAACCTGCC TGGCGAGCAC GGGCAGAGGC TGCCCATCAG CAGGAACACC
    TTTGCAGAAG TCACCGGGCT GTCCCCTGGG GTCACCTATT ACTTCAAAGT CTTTGCAGTG
    AGCCATGGGA GGGAGAGCAA GCCTCTGACT GCTCAACAGA CAACCAAACT GGATGCTCCC
    ACTAACCTCC AGTTTGTCAA TGAAACTGAT TCTACTGTCC TGGTGAGATG GACTCCACCT
    CGGGCCCAGA TAACAGGATA CCGACTGACC GTGGGCCTTA CCCGAAGAGG ACAGCCCAGG
    CAGTACAATG TGGGTCCCTC TGTCTCCAAG TACCCACTGA GGAATCTGCA GCCTGCATCT
    GAGTACACCG TATCCCTCGT GGCCATAAAG GGCAACCAAG AGAGCCCCAA AGCCACTGGA
    GTCTTTACCA CACTGCAGCC TGGGAGCTCT ATTCCACCTT ACAACACCGA GGTGACTGAG
    ACCACCATTG TGATCACATG GACGCCTGCT CCAAGAATTG GTTTTAAGCT GGGTGTACGA
    CCAAGCCAGG GAGGAGAGGC ACCACGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG
    TCCGGCTTGA CTCCAGGAGT AGAATACGTC TACACCATCC AAGTCCTGAG AGATGGACAG
    GAAAGAGATG CGCCAATTGT AAACAAAGTG GTGACACCAT TGTCTCCACC AACAAACTTG
    CATCTGGAGG CAAACCCTGA CACTGGAGTG CTCACAGTCT CCTGGGAGAG GAGCACCACC
    CCAGACATTA CTGGTTATAG AATTACCACA ACCCCTACAA ACGGCCAGCA GGGAAATTCT
    TTGGAAGAAG TGGTCCATGC TGATCAGAGC TCCTGCACTT TTGATAACCT GAGTCCCGGC
    CTGGAGTACA ATGTCAGTGT TTACACTGTC AAGGATGACA AGGAAAGTGT CCCTATCTCT
    GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT
    TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTACCGCATC
    ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA
    GGATACTACA CAGTCACAGG GCTGGAGCCG GGCATTGACT ATGATATCAG CGTTATCACT
    CTCATTAATG GCGGCGAGAG TGCCCCTACT ACACTGACAC AACAAACGGC TGTTCCTCCT
    CCCACTGACC TGCGATTCAC CAACATTGGT CCAGACACCA TGCGTGTCAC CTGGGCTCCA
    CCCCCATCCA TTGATTTAAC CAACTTCCTG GTGCGTTACT CACCTGTGAA AAATGAGGAA
    GATGTTGCAG AGTTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG
    CCTGGTACAG AATATGTAGT GAGTGTCTCC AGTGTCTACG AACAACATGA GAGCACACCT
    CTTAGAGGAA GACAGAAAAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATT
    ACTGCCAACT CTTTTACTGT GCACTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG
    ATCCGCCATC ATCCCGAGCA CTTCAGTGGG AGACCTCGAG AAGATCGGGT GCCCCACTCT
    CGGAATTCCA TCACCCTCAC CAACCTCACT CCAGGCACAG AGTATGTGGT CAGCATCGTT
    GCTCTTAATG GCAGAGAGGA AAGTCCCTTA TTGATTGGCC AACAATCAAC AGTTTCTGAT
    GTTCCGAGGG ACCTGGAAGT TGTTGCTGCG ACCCCCACCA GCCTACTGAT CAGCTGGGAT
    GCTCCTGCTG TCACAGTGAG ATATTACAGG ATCACTTACG GAGAGACAGG AGGAAATAGC
    CCTGTCCAGG AGTTCACTGT GCCTGGGAGC AAGTCTACAG CTACCATCAG CGGCCTTAAA
    CCTGGAGTTG ATTATACCAT CACTGTGTAT GCTGTCACTG GCCGTGGAGA CAGCCCCGCA
    AGCAGCAAGC CAATTTCCAT TAATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA
    GTGACCGATG TTCAGGACAA CAGCATTAGT GTCAAGTGGC TGCCTTCAAG TTCCCCTGTT
    ACTGGTTACA GAGTAACCAC CACTCCCAAA AATGGACCAG GACCAACAAA AACTAAAACT
    GCAGGTCCAG ATCAAACAGA AATGACTATT GAAGGCTTGC AGCCCACAGT GGAGTATGTG
    GTTAGTGTCT ATGCTCAGAA TCCAAGCGGA GAGAGTCAGC CTCTGGTTCA GACTGCAGTA
    ACCAACATTG ATCGCCCTAA AGGACTGGCA TTCACTGATG TGGATGTCGA TTCCATCAAA
    ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT
    GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG
    CAAGGCCTCA GACCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG
    GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCTATTC CTGCACCAAC TGACCTGAAG
    TTCACTCAGG TCACACCCAC AAGCCTGAGC GCCCAGTGGA CACCACCCAA TGTTCAGCTC
    ACTGGATATC GAGTGCGGGT GACCCCCAAG GAGAAGACCG GACCAATGAA AGAAATCAAC
    CTTGCTCCTG ACAGCTCATC CGTGGTTGTA TCAGGACTTA TGGTGGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CAGCTCAGGG AGTTGTCACC
    ACTCTGGAGA ATGTCAGCCC ACCAAGAAGG GCTCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC CAAGACTGAG ACGATCACTG GCTTCCAAGT TGATGCCGTT
    CCAGCCAATG GCCAGACTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC
    ATCACAGGTT TACAACCAGG CACTGACTAC AAGATCTACC TGTACACCTT GAATGACAAT
    GCTCGGAGCT CCCCTGTGGT CATCGACGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAATTCCTTG CTGGTATCAT GGCAGCCGCC ACGTGCCAGG
    ATTACCGGCT ACATCATCAA GTATGAGAAG CCTGGGTCTC CTCCCAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAGGCTACT ATTACTGGCC TGGAACCGGG AACCGAATAT
    ACAATTTATG TCATTGCCCT GAAGAATAAT CAGAAGAGCG AGCCCCTGAT TGGAAGGAAA
    AAGACAGACG AGCTTCCCCA ACTGGTAACC CTTCCACACC CCAATCTTCA TGGACCAGAG
    ATCTTGGATG TTCCTTCCAC AGTTCAAAAG ACCCCTTTCG TCACCCACCC TGGGTATGAC
    ACTGGAAATG GTATTCAGCT TCCTGGCACT TCTGGTCAGC AACCCAGTGT TGGGCAACAA
    ATGATCTTTG AGGAACATGG TTTTAGGCGG ACCACACCGC CCACAACGGC CACCCCCATA
    AGGCATAGGC CAAGACCATA CCCGCCGAAT GTAGGACAAG AAGCTCTCTC TCAGACAACC
    ATCTCATGGG CCCCATTCCA GGACACTTCT GAGTACATCA TTTCATGTCA TCCTGTTGGC
    ACTGATGAAG AACCCTTACA GTTCAGGGTT CCTGGAACTT CTACCAGTGC CACTCTGACA
    GGCCTCACCA GAGGTGCCAC CTACAACGTC ATAGTGGAGG CACTGAAAGA CCAGCAGAGG
    CATAAGGTTC GGGAAGAGGT TGTTACCGTG GGCAACTCTG TCAACGAAGG CTTGAACCAA
    CCTACGGATG ACTCGTGCTT TGACCCCTAC ACAGTTTCCC ATTATGCCGT TGGAGATGAG
    TGGGAACGAA TGTCTGAATC AGGCTTTAAA CTGTTGTGCC AGTGCTTAGG CTTTGGAAGT
    GGTCATTTCA GATGTGATTC ATCTAGATGG TGCCATGACA ATGGTGTGAA CTACAAGATT
    GGAGAGAAGT GGGACCGTCA GGGAGAAAAT GGCCAGATGA TGAGCTGCAC ATGTCTTGGG
    AACGGAAAAG GAGAATTCAA GTGTGACCCT CATGAGGCAA CGTGTTATGA TGATGGGAAG
    ACATACCACG TAGGAGAACA GTGGCAGAAG GAATATCTCG GTGCCATTTG CTCCTGCACA
    TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT GACAACTGCC GCAGACCTGG GGGTGAACCC
    AGTCCCGAAG GCACTACTGG CCAGTCCTAC AACCAGTATT CTCAGAGATA CCATCAGAGA
    ACAAACACTG TAAGTGCATT AGCAGCACAA GTGTGTTCCC TCATACTAGA CAGTCTCTTT
    CTACAGGTAT CTTTCTTCAG AATGAACCAA GTGTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005246456.1
    CDS366..7724
    Translation

    Target ORF information:

    RefSeq Version XM_005246399.1
    Organism Homo sapiens(human)
    Definition Homo sapiens fibronectin 1 (FN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005246399.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGCTTAGGG GTCCGGGGCC CGGGCTGCTG CTGCTGGCCG TCCAGTGCCT GGGGACAGCG 
    GTGCCCTCCA CGGGAGCCTC GAAGAGCAAG AGGCAGGCTC AGCAAATGGT TCAGCCCCAG
    TCCCCGGTGG CTGTCAGTCA AAGCAAGCCC GGTTGTTATG ACAATGGAAA ACACTATCAG
    ATAAATCAAC AGTGGGAGCG GACCTACCTA GGCAATGCGT TGGTTTGTAC TTGTTATGGA
    GGAAGCCGAG GTTTTAACTG CGAGAGTAAA CCTGAAGCTG AAGAGACTTG CTTTGACAAG
    TACACTGGGA ACACTTACCG AGTGGGTGAC ACTTATGAGC GTCCTAAAGA CTCCATGATC
    TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATAA GCTGTACCAT CGCAAACCGC
    TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC ACATGAGACT
    GGTGGTTACA TGTTAGAGTG TGTGTGTCTT GGTAATGGAA AAGGAGAATG GACCTGCAAG
    CCCATAGCTG AGAAGTGTTT TGATCATGCT GCTGGGACTT CCTATGTGGT CGGAGAAACG
    TGGGAGAAGC CCTACCAAGG CTGGATGATG GTAGATTGTA CTTGCCTGGG AGAAGGCAGC
    GGACGCATCA CTTGCACTTC TAGAAATAGA TGCAACGATC