NCSTN cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following NCSTN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCSTN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu23623 NM_015331.3
Latest version!
Homo sapiens nicastrin (NCSTN), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$354.50-$496.30
$709.00
OHu08810 NM_001290186.2
Latest version!
Homo sapiens nicastrin (NCSTN), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu66123 NM_001349729.2
Latest version!
Homo sapiens nicastrin (NCSTN), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu34278 XM_005245053.5
Latest version!
Homo sapiens nicastrin (NCSTN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu08813 NM_001290184.2
Latest version!
Homo sapiens nicastrin (NCSTN), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
View Old Accession Versions >>
OHu08810 NM_001290186.1 Homo sapiens nicastrin (NCSTN), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu23623 NM_015331.2 Homo sapiens nicastrin (NCSTN), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$354.50-$496.30
$709.00
OHu08813 NM_001290184.1 Homo sapiens nicastrin (NCSTN), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu66123 NM_001349729.1 Homo sapiens nicastrin (NCSTN), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu34278 XM_005245053.4 Homo sapiens nicastrin (NCSTN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu23623
    Clone ID Related Accession (Same CDS sequence) NM_015331.2 , NM_015331.3
    Accession Version NM_015331.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2130bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-15
    Organism Homo sapiens(human)
    Product nicastrin isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF240468.1, BC047621.1 and CN429672.1. This sequence is a reference standard in the RefSeqGene project. On Apr 14, 2005 this sequence version replaced NM_015331.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.58775.1, AF240468.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTACGG CAGGGGGTGG CTCTGGGGCT GACCCGGGAA GTCGGGGTCT CCTTCGCCTT 
    CTGTCTTTCT GCGTCCTACT AGCAGGTTTG TGCAGGGGAA ACTCAGTGGA GAGGAAGATA
    TATATCCCCT TAAATAAAAC AGCTCCCTGT GTTCGCCTGC TCAACGCCAC TCATCAGATT
    GGCTGCCAGT CTTCAATTAG TGGAGACACA GGGGTTATCC ACGTAGTAGA GAAAGAGGAG
    GACCTACAGT GGGTATTGAC TGATGGCCCC AACCCCCCTT ACATGGTTCT GCTGGAGAGC
    AAGCATTTTA CCAGGGATTT AATGGAGAAG CTGAAAGGGA GAACCAGCCG AATTGCTGGT
    CTTGCAGTGT CCTTGACCAA GCCCAGTCCT GCCTCAGGCT TCTCTCCTAG TGTACAGTGC
    CCAAATGATG GGTTTGGTGT TTACTCCAAT TCCTATGGGC CAGAGTTTGC TCACTGCAGA
    GAAATACAGT GGAATTCGCT GGGCAATGGT TTGGCTTATG AAGACTTTAG TTTCCCCATC
    TTTCTTCTTG AAGATGAAAA TGAAACCAAA GTCATCAAGC AGTGCTATCA AGATCACAAC
    CTGAGTCAGA ATGGCTCAGC ACCAACCTTC CCACTATGTG CCATGCAGCT CTTTTCACAC
    ATGCATGCTG TCATCAGCAC TGCCACCTGC ATGCGGCGCA GCTCCATCCA AAGCACCTTC
    AGCATCAACC CAGAAATCGT CTGTGACCCC CTGTCTGATT ACAATGTGTG GAGCATGCTA
    AAGCCTATAA ATACAACTGG GACATTAAAG CCTGACGACA GGGTTGTGGT TGCTGCCACC
    CGGCTGGATA GTCGTTCCTT TTTCTGGAAT GTGGCCCCAG GGGCTGAAAG CGCAGTGGCT
    TCCTTTGTCA CCCAGCTGGC TGCTGCTGAA GCTTTGCAAA AGGCACCTGA TGTGACCACC
    CTGCCCCGCA ATGTCATGTT TGTCTTCTTT CAAGGGGAAA CTTTTGACTA CATTGGCAGC
    TCGAGGATGG TCTACGATAT GGAGAAGGGC AAGTTTCCCG TGCAGTTAGA GAATGTTGAC
    TCATTTGTGG AGCTGGGACA GGTGGCCTTA AGAACTTCAT TAGAGCTTTG GATGCACACA
    GATCCTGTTT CTCAGAAAAA TGAGTCTGTA CGGAACCAGG TGGAGGATCT CCTGGCCACA
    TTGGAGAAGA GTGGTGCTGG TGTCCCTGCT GTCATCCTCA GGAGGCCAAA TCAGTCCCAG
    CCTCTCCCAC CATCTTCCCT GCAGCGATTT CTTCGAGCTC GAAACATCTC TGGCGTTGTT
    CTGGCTGACC ACTCTGGTGC CTTCCATAAC AAATATTACC AGAGTATTTA CGACACTGCT
    GAGAACATTA ATGTGAGCTA TCCCGAATGG CTGAGCCCTG AAGAGGACCT GAACTTTGTA
    ACAGACACTG CCAAGGCCCT GGCAGATGTG GCCACGGTGC TGGGACGTGC TCTGTATGAG
    CTTGCAGGAG GAACCAACTT CAGCGACACA GTTCAGGCTG ATCCCCAAAC GGTTACCCGC
    CTGCTCTATG GGTTCCTGAT TAAAGCCAAC AACTCATGGT TCCAGTCTAT CCTCAGGCAG
    GACCTAAGGT CCTACTTGGG TGACGGGCCT CTTCAACATT ACATCGCTGT CTCCAGCCCC
    ACCAACACCA CTTATGTTGT ACAGTATGCC TTGGCAAATT TGACTGGCAC AGTGGTCAAC
    CTCACCCGAG AGCAGTGCCA GGATCCAAGT AAAGTCCCAA GTGAAAACAA GGATCTGTAT
    GAGTACTCAT GGGTCCAGGG CCCTTTGCAT TCTAATGAGA CGGACCGACT CCCCCGGTGT
    GTGCGTTCTA CTGCACGATT AGCCAGGGCC TTGTCTCCTG CCTTTGAACT GAGTCAGTGG
    AGCTCTACTG AATACTCTAC ATGGACTGAG AGCCGCTGGA AAGATATCCG TGCCCGGATA
    TTTCTCATCG CCAGCAAAGA GCTTGAGTTG ATCACCCTGA CAGTGGGCTT CGGCATCCTC
    ATCTTCTCCC TCATCGTCAC CTACTGCATC AATGCCAAAG CTGATGTCCT TTTCATTGCT
    CCCCGGGAGC CAGGAGCTGT GTCATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_056146.1
    CDS125..2254
    Translation

    Target ORF information:

    RefSeq Version NM_015331.2
    Organism Homo sapiens(human)
    Definition Homo sapiens nicastrin (NCSTN), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_015331.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTACGG CAGGGGGTGG CTCTGGGGCT GACCCGGGAA GTCGGGGTCT CCTTCGCCTT 
    CTGTCTTTCT GCGTCCTACT AGCAGGTTTG TGCAGGGGAA ACTCAGTGGA GAGGAAGATA
    TATATCCCCT TAAATAAAAC AGCTCCCTGT GTTCGCCTGC TCAACGCCAC TCATCAGATT
    GGCTGCCAGT CTTCAATTAG TGGAGACACA GGGGTTATCC ACGTAGTAGA GAAAGAGGAG
    GACCTACAGT GGGTATTGAC TGATGGCCCC AACCCCCCTT ACATGGTTCT GCTGGAGAGC
    AAGCATTTTA CCAGGGATTT AATGGAGAAG CTGAAAGGGA GAACCAGCCG AATTGCTGGT
    CTTGCAGTGT CCTTGACCAA GCCCAGTCCT GCCTCAGGCT TCTCTCCTAG TGTACAGTGC
    CCAAATGATG GGTTTGGTGT TTACTCCAAT TCCTATGGGC CAGAGTTTGC TCACTGCAGA
    GAAATACAGT GGAATTCGCT GGGCAATGGT TTGGCTTATG AAGACTTTAG TTTCCCCATC
    TTTCTTCTTG AAGATGAAAA TGAAACCAAA GTCATCAAGC AGTGCTATCA AGATCACAAC
    CTGAGTCAGA ATGGCTCAGC ACCAACCTTC CCACTATGTG CCATGCAGCT CTTTTCACAC
    ATGCATGCTG TCATCAGCAC TGCCACCTGC ATGCGGCGCA GCTCCATCCA AAGCACCTTC
    AGCATCAACC CAGAAATCGT CTGTGACCCC CTGTCTGATT ACAATGTGTG GAGCATGCTA
    AAGCCTATAA ATACAACTGG GACATTAAAG CCTGACGACA GGGTTGTGGT TGCTGCCACC
    CGGCTGGATA GTCGTTCCTT TTTCTGGAAT GTGGCCCCAG GGGCTGAAAG CGCAGTGGCT
    TCCTTTGTCA CCCAGCTGGC TGCTGCTGAA GCTTTGCAAA AGGCACCTGA TGTGACCACC
    CTGCCCCGCA ATGTCATGTT TGTCTTCTTT CAAGGGGAAA CTTTTGACTA CATTGGCAGC
    TCGAGGATGG TCTACGATAT GGAGAAGGGC AAGTTTCCCG TGCAGTTAGA GAATGTTGAC
    TCATTTGTGG AGCTGGGACA GGTGGCCTTA AGAACTTCAT TAGAGCTTTG GATGCACACA
    GATCCTGTTT CTCAGAAAAA TGAGTCTGTA CGGAACCAGG TGGAGGATCT CCTGGCCACA
    TTGGAGAAGA GTGGTGCTGG TGTCCCTGCT GTCATCCTCA GGAGGCCAAA TCAGTCCCAG
    CCTCTCCCAC CATCTTCCCT GCAGCGATTT CTTCGAGCTC GAAACATCTC TGGCGTTGTT
    CTGGCTGACC ACTCTGGTGC CTTCCATAAC AAATATTACC AGAGTATTTA CGACACTGCT
    GAGAACATTA ATGTGAGCTA TCCCGAATGG CTGAGCCCTG AAGAGGACCT GAACTTTGTA
    ACAGACACTG CCAAGGCCCT GGCAGATGTG GCCACGGTGC TGGGACGTGC TCTGTATGAG
    CTTGCAGGAG GAACCAACTT CAGCGACACA GTTCAGGCTG ATCCCCAAAC GGTTACCCGC
    CTGCTCTATG GGTTCCTGAT TAAAGCCAAC AACTCATGGT TCCAGTCTAT CCTCAGGCAG
    GACCTAAGGT CCTACTTGGG TGACGGGCCT CTTCAACATT ACATCGCTGT CTCCAGCCCC
    ACCAACACCA CTTATGTTGT ACAGTATGCC TTGGCAAATT TGACTGGCAC AGTGGTCAAC
    CTCACCCGAG AGCAGTGCCA GGATCCAAGT AAAGTCCCAA GTGAAAACAA GGATCTGTAT
    GAGTACTCAT GGGTCCAGGG CCCTTTGCAT TCTAATGAGA CGGACCGACT CCCCCGGTGT
    GTGCGTTCTA CTGCACGATT AGCCAGGGCC TTGTCTCCTG CCTTTGAACT GAGTCAGTGG
    AGCTCTACTG AATACTCTAC ATGGACTGAG AGCCGCTGGA AAGATATCCG TGCCCGGATA
    TTTCTCATCG CCAGCAAAGA GCTTGAGTTG ATCACCCTGA CAGTGGGCTT CGGCATCCTC
    ATCTTCTCCC TCATCGTCAC CTACTGCATC AATGCCAAAG CTGATGTCCT TTTCATTGCT
    CCCCGGGAGC CAGGAGCTGT GTCATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23623
    Clone ID Related Accession (Same CDS sequence) NM_015331.2 , NM_015331.3
    Accession Version NM_015331.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2130bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product nicastrin isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF240468.1, BC047621.1 and CN429672.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_015331.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.580341.1, AF240468.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000294785.10/ ENSP00000294785.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTACGG CAGGGGGTGG CTCTGGGGCT GACCCGGGAA GTCGGGGTCT CCTTCGCCTT 
    CTGTCTTTCT GCGTCCTACT AGCAGGTTTG TGCAGGGGAA ACTCAGTGGA GAGGAAGATA
    TATATCCCCT TAAATAAAAC AGCTCCCTGT GTTCGCCTGC TCAACGCCAC TCATCAGATT
    GGCTGCCAGT CTTCAATTAG TGGAGACACA GGGGTTATCC ACGTAGTAGA GAAAGAGGAG
    GACCTACAGT GGGTATTGAC TGATGGCCCC AACCCCCCTT ACATGGTTCT GCTGGAGAGC
    AAGCATTTTA CCAGGGATTT AATGGAGAAG CTGAAAGGGA GAACCAGCCG AATTGCTGGT
    CTTGCAGTGT CCTTGACCAA GCCCAGTCCT GCCTCAGGCT TCTCTCCTAG TGTACAGTGC
    CCAAATGATG GGTTTGGTGT TTACTCCAAT TCCTATGGGC CAGAGTTTGC TCACTGCAGA
    GAAATACAGT GGAATTCGCT GGGCAATGGT TTGGCTTATG AAGACTTTAG TTTCCCCATC
    TTTCTTCTTG AAGATGAAAA TGAAACCAAA GTCATCAAGC AGTGCTATCA AGATCACAAC
    CTGAGTCAGA ATGGCTCAGC ACCAACCTTC CCACTATGTG CCATGCAGCT CTTTTCACAC
    ATGCATGCTG TCATCAGCAC TGCCACCTGC ATGCGGCGCA GCTCCATCCA AAGCACCTTC
    AGCATCAACC CAGAAATCGT CTGTGACCCC CTGTCTGATT ACAATGTGTG GAGCATGCTA
    AAGCCTATAA ATACAACTGG GACATTAAAG CCTGACGACA GGGTTGTGGT TGCTGCCACC
    CGGCTGGATA GTCGTTCCTT TTTCTGGAAT GTGGCCCCAG GGGCTGAAAG CGCAGTGGCT
    TCCTTTGTCA CCCAGCTGGC TGCTGCTGAA GCTTTGCAAA AGGCACCTGA TGTGACCACC
    CTGCCCCGCA ATGTCATGTT TGTCTTCTTT CAAGGGGAAA CTTTTGACTA CATTGGCAGC
    TCGAGGATGG TCTACGATAT GGAGAAGGGC AAGTTTCCCG TGCAGTTAGA GAATGTTGAC
    TCATTTGTGG AGCTGGGACA GGTGGCCTTA AGAACTTCAT TAGAGCTTTG GATGCACACA
    GATCCTGTTT CTCAGAAAAA TGAGTCTGTA CGGAACCAGG TGGAGGATCT CCTGGCCACA
    TTGGAGAAGA GTGGTGCTGG TGTCCCTGCT GTCATCCTCA GGAGGCCAAA TCAGTCCCAG
    CCTCTCCCAC CATCTTCCCT GCAGCGATTT CTTCGAGCTC GAAACATCTC TGGCGTTGTT
    CTGGCTGACC ACTCTGGTGC CTTCCATAAC AAATATTACC AGAGTATTTA CGACACTGCT
    GAGAACATTA ATGTGAGCTA TCCCGAATGG CTGAGCCCTG AAGAGGACCT GAACTTTGTA
    ACAGACACTG CCAAGGCCCT GGCAGATGTG GCCACGGTGC TGGGACGTGC TCTGTATGAG
    CTTGCAGGAG GAACCAACTT CAGCGACACA GTTCAGGCTG ATCCCCAAAC GGTTACCCGC
    CTGCTCTATG GGTTCCTGAT TAAAGCCAAC AACTCATGGT TCCAGTCTAT CCTCAGGCAG
    GACCTAAGGT CCTACTTGGG TGACGGGCCT CTTCAACATT ACATCGCTGT CTCCAGCCCC
    ACCAACACCA CTTATGTTGT ACAGTATGCC TTGGCAAATT TGACTGGCAC AGTGGTCAAC
    CTCACCCGAG AGCAGTGCCA GGATCCAAGT AAAGTCCCAA GTGAAAACAA GGATCTGTAT
    GAGTACTCAT GGGTCCAGGG CCCTTTGCAT TCTAATGAGA CGGACCGACT CCCCCGGTGT
    GTGCGTTCTA CTGCACGATT AGCCAGGGCC TTGTCTCCTG CCTTTGAACT GAGTCAGTGG
    AGCTCTACTG AATACTCTAC ATGGACTGAG AGCCGCTGGA AAGATATCCG TGCCCGGATA
    TTTCTCATCG CCAGCAAAGA GCTTGAGTTG ATCACCCTGA CAGTGGGCTT CGGCATCCTC
    ATCTTCTCCC TCATCGTCAC CTACTGCATC AATGCCAAAG CTGATGTCCT TTTCATTGCT
    CCCCGGGAGC CAGGAGCTGT GTCATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_056146.1
    CDS15..2144
    Translation

    Target ORF information:

    RefSeq Version NM_015331.3
    Organism Homo sapiens(human)
    Definition Homo sapiens nicastrin (NCSTN), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_015331.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTACGG CAGGGGGTGG CTCTGGGGCT GACCCGGGAA GTCGGGGTCT CCTTCGCCTT 
    CTGTCTTTCT GCGTCCTACT AGCAGGTTTG TGCAGGGGAA ACTCAGTGGA GAGGAAGATA
    TATATCCCCT TAAATAAAAC AGCTCCCTGT GTTCGCCTGC TCAACGCCAC TCATCAGATT
    GGCTGCCAGT CTTCAATTAG TGGAGACACA GGGGTTATCC ACGTAGTAGA GAAAGAGGAG
    GACCTACAGT GGGTATTGAC TGATGGCCCC AACCCCCCTT ACATGGTTCT GCTGGAGAGC
    AAGCATTTTA CCAGGGATTT AATGGAGAAG CTGAAAGGGA GAACCAGCCG AATTGCTGGT
    CTTGCAGTGT CCTTGACCAA GCCCAGTCCT GCCTCAGGCT TCTCTCCTAG TGTACAGTGC
    CCAAATGATG GGTTTGGTGT TTACTCCAAT TCCTATGGGC CAGAGTTTGC TCACTGCAGA
    GAAATACAGT GGAATTCGCT GGGCAATGGT TTGGCTTATG AAGACTTTAG TTTCCCCATC
    TTTCTTCTTG AAGATGAAAA TGAAACCAAA GTCATCAAGC AGTGCTATCA AGATCACAAC
    CTGAGTCAGA ATGGCTCAGC ACCAACCTTC CCACTATGTG CCATGCAGCT CTTTTCACAC
    ATGCATGCTG TCATCAGCAC TGCCACCTGC ATGCGGCGCA GCTCCATCCA AAGCACCTTC
    AGCATCAACC CAGAAATCGT CTGTGACCCC CTGTCTGATT ACAATGTGTG GAGCATGCTA
    AAGCCTATAA ATACAACTGG GACATTAAAG CCTGACGACA GGGTTGTGGT TGCTGCCACC
    CGGCTGGATA GTCGTTCCTT TTTCTGGAAT GTGGCCCCAG GGGCTGAAAG CGCAGTGGCT
    TCCTTTGTCA CCCAGCTGGC TGCTGCTGAA GCTTTGCAAA AGGCACCTGA TGTGACCACC
    CTGCCCCGCA ATGTCATGTT TGTCTTCTTT CAAGGGGAAA CTTTTGACTA CATTGGCAGC
    TCGAGGATGG TCTACGATAT GGAGAAGGGC AAGTTTCCCG TGCAGTTAGA GAATGTTGAC
    TCATTTGTGG AGCTGGGACA GGTGGCCTTA AGAACTTCAT TAGAGCTTTG GATGCACACA
    GATCCTGTTT CTCAGAAAAA TGAGTCTGTA CGGAACCAGG TGGAGGATCT CCTGGCCACA
    TTGGAGAAGA GTGGTGCTGG TGTCCCTGCT GTCATCCTCA GGAGGCCAAA TCAGTCCCAG
    CCTCTCCCAC CATCTTCCCT GCAGCGATTT CTTCGAGCTC GAAACATCTC TGGCGTTGTT
    CTGGCTGACC ACTCTGGTGC CTTCCATAAC AAATATTACC AGAGTATTTA CGACACTGCT
    GAGAACATTA ATGTGAGCTA TCCCGAATGG CTGAGCCCTG AAGAGGACCT GAACTTTGTA
    ACAGACACTG CCAAGGCCCT GGCAGATGTG GCCACGGTGC TGGGACGTGC TCTGTATGAG
    CTTGCAGGAG GAACCAACTT CAGCGACACA GTTCAGGCTG ATCCCCAAAC GGTTACCCGC
    CTGCTCTATG GGTTCCTGAT TAAAGCCAAC AACTCATGGT TCCAGTCTAT CCTCAGGCAG
    GACCTAAGGT CCTACTTGGG TGACGGGCCT CTTCAACATT ACATCGCTGT CTCCAGCCCC
    ACCAACACCA CTTATGTTGT ACAGTATGCC TTGGCAAATT TGACTGGCAC AGTGGTCAAC
    CTCACCCGAG AGCAGTGCCA GGATCCAAGT AAAGTCCCAA GTGAAAACAA GGATCTGTAT
    GAGTACTCAT GGGTCCAGGG CCCTTTGCAT TCTAATGAGA CGGACCGACT CCCCCGGTGT
    GTGCGTTCTA CTGCACGATT AGCCAGGGCC TTGTCTCCTG CCTTTGAACT GAGTCAGTGG
    AGCTCTACTG AATACTCTAC ATGGACTGAG AGCCGCTGGA AAGATATCCG TGCCCGGATA
    TTTCTCATCG CCAGCAAAGA GCTTGAGTTG ATCACCCTGA CAGTGGGCTT CGGCATCCTC
    ATCTTCTCCC TCATCGTCAC CTACTGCATC AATGCCAAAG CTGATGTCCT TTTCATTGCT
    CCCCGGGAGC CAGGAGCTGT GTCATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08810
    Clone ID Related Accession (Same CDS sequence) NM_001290186.2 , NM_001290186.1
    Accession Version NM_001290186.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1716bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product nicastrin isoform 3 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF240468.1, AK299142.1, AL445230.11 and CN429672.1. On May 31, 2019 this sequence version replaced NM_001290186.1. Transcript Variant: This variant (3) lacks three alternate in-frame exons in the coding region compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.918758.1, SRR1660805.11658.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCTACGG CAGGGGGTGG CTCTGGGGCT GACCCGGGAA GTCGGGGTCT CCTTCGCCTT 
    CTGTCTTTCT GCGTCCTACT AGCAGGTTTG TGCAGGGGAA ACTCAGTGGA GAGGAAGATA
    TATATCCCCT TAAATAAAAC AGCTCCCTGT GTTCGCCTGC TCAACGCCAC TCATCAGATT
    GGCTGCCAGT CTTCAATTAG TGGAGACACA GGGGTTATCC ACGTAGTAGA GAAAGAGGAG
    GACCTACAGT GGGTATTGAC TGATGGCCCC AACCCCCCTT ACATGGTTCT GCTGGAGAGC
    AAGCATTTTA CCAGGGATTT AATGGAGAAG CTGAAAGGGA GAACCAGCCG AATTGCTGGT
    CTTGCAGTGT CCTTGACCAA GCCCAGTCCT GCCTCAGGCT TCTCTCCTAG TGTACAGTGC
    CCAAATGATG GGTTTGGTGT TTACTCCAAT TCCTATGGGC CAGAGTTTGC TCACTGCAGA
    GAAATACAGT GGAATTCGCT GGGCAATGGT TTGGCTTATG AAGACTTTAG TTTCCCCATC
    TTTCTTCTTG AAGATGAAAA TGAAACCAAA GTCATCAAGC AGGAAACTTT TGACTACATT
    GGCAGCTCGA GGATGGTCTA CGATATGGAG AAGGGCAAGT TTCCCGTGCA GTTAGAGAAT
    GTTGACTCAT TTGTGGAGCT GGGACAGGTG GCCTTAAGAA CTTCATTAGA GCTTTGGATG
    CACACAGATC CTGTTTCTCA GAAAAATGAG TCTGTACGGA ACCAGGTGGA GGATCTCCTG
    GCCACATTGG AGAAGAGTGG TGCTGGTGTC CCTGCTGTCA TCCTCAGGAG GCCAAATCAG
    TCCCAGCCTC TCCCACCATC TTCCCTGCAG CGATTTCTTC GAGCTCGAAA CATCTCTGGC
    GTTGTTCTGG CTGACCACTC TGGTGCCTTC CATAACAAAT ATTACCAGAG TATTTACGAC
    ACTGCTGAGA ACATTAATGT GAGCTATCCC GAATGGCTGA GCCCTGAAGA GGACCTGAAC
    TTTGTAACAG ACACTGCCAA GGCCCTGGCA GATGTGGCCA CGGTGCTGGG ACGTGCTCTG
    TATGAGCTTG CAGGAGGAAC CAACTTCAGC GACACAGTTC AGGCTGATCC CCAAACGGTT
    ACCCGCCTGC TCTATGGGTT CCTGATTAAA GCCAACAACT CATGGTTCCA GTCTATCCTC
    AGGCAGGACC TAAGGTCCTA CTTGGGTGAC GGGCCTCTTC AACATTACAT CGCTGTCTCC
    AGCCCCACCA ACACCACTTA TGTTGTACAG TATGCCTTGG CAAATTTGAC TGGCACAGTG
    GTCAACCTCA CCCGAGAGCA GTGCCAGGAT CCAAGTAAAG TCCCAAGTGA AAACAAGGAT
    CTGTATGAGT ACTCATGGGT CCAGGGCCCT TTGCATTCTA ATGAGACGGA CCGACTCCCC
    CGGTGTGTGC GTTCTACTGC ACGATTAGCC AGGGCCTTGT CTCCTGCCTT TGAACTGAGT
    CAGTGGAGCT CTACTGAATA CTCTACATGG ACTGAGAGCC GCTGGAAAGA TATCCGTGCC
    CGGATATTTC TCATCGCCAG CAAAGAGCTT GAGTTGATCA CCCTGACAGT GGGCTTCGGC
    ATCCTCATCT TCTCCCTCAT CGTCACCTAC TGCATCAATG CCAAAGCTGA TGTCCTTTTC
    ATTGCTCCCC GGGAGCCAGG AGCTGTGTCA TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001277115.1
    CDS15..1730
    Translation

    Target ORF information:

    RefSeq Version NM_001290186.2
    Organism Homo sapiens(human)
    Definition Homo sapiens nicastrin (NCSTN), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001290186.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCTACGG CAGGGGGTGG CTCTGGGGCT GACCCGGGAA GTCGGGGTCT CCTTCGCCTT 
    CTGTCTTTCT GCGTCCTACT AGCAGGTTTG TGCAGGGGAA ACTCAGTGGA GAGGAAGATA
    TATATCCCCT TAAATAAAAC AGCTCCCTGT GTTCGCCTGC TCAACGCCAC TCATCAGATT
    GGCTGCCAGT CTTCAATTAG TGGAGACACA GGGGTTATCC ACGTAGTAGA GAAAGAGGAG
    GACCTACAGT GGGTATTGAC TGATGGCCCC AACCCCCCTT ACATGGTTCT GCTGGAGAGC
    AAGCATTTTA CCAGGGATTT AATGGAGAAG CTGAAAGGGA GAACCAGCCG AATTGCTGGT
    CTTGCAGTGT CCTTGACCAA GCCCAGTCCT GCCTCAGGCT TCTCTCCTAG TGTACAGTGC
    CCAAATGATG GGTTTGGTGT TTACTCCAAT TCCTATGGGC CAGAGTTTGC TCACTGCAGA
    GAAATACAGT GGAATTCGCT GGGCAATGGT TTGGCTTATG AAGACTTTAG TTTCCCCATC
    TTTCTTCTTG AAGATGAAAA TGAAACCAAA GTCATCAAGC AGGAAACTTT TGACTACATT
    GGCAGCTCGA GGATGGTCTA CGATATGGAG AAGGGCAAGT TTCCCGTGCA GTTAGAGAAT
    GTTGACTCAT TTGTGGAGCT GGGACAGGTG GCCTTAAGAA CTTCATTAGA GCTTTGGATG
    CACACAGATC CTGTTTCTCA GAAAAATGAG TCTGTACGGA ACCAGGTGGA GGATCTCCTG
    GCCACATTGG AGAAGAGTGG TGCTGGTGTC CCTGCTGTCA TCCTCAGGAG GCCAAATCAG
    TCCCAGCCTC TCCCACCATC TTCCCTGCAG CGATTTCTTC GAGCTCGAAA CATCTCTGGC
    GTTGTTCTGG CTGACCACTC TGGTGCCTTC CATAACAAAT ATTACCAGAG TATTTACGAC
    ACTGCTGAGA ACATTAATGT GAGCTATCCC GAATGGCTGA GCCCTGAAGA GGACCTGAAC
    TTTGTAACAG ACACTGCCAA GGCCCTGGCA GATGTGGCCA CGGTGCTGGG ACGTGCTCTG
    TATGAGCTTG CAGGAGGAAC CAACTTCAGC GACACAGTTC AGGCTGATCC CCAAACGGTT
    ACCCGCCTGC TCTATGGGTT CCTGATTAAA GCCAACAACT CATGGTTCCA GTCTATCCTC
    AGGCAGGACC TAAGGTCCTA CTTGGGTGAC GGGCCTCTTC AACATTACAT CGCTGTCTCC
    AGCCCCACCA ACACCACTTA TGTTGTACAG TATGCCTTGG CAAATTTGAC TGGCACAGTG
    GTCAACCTCA CCCGAGAGCA GTGCCAGGAT CCAAGTAAAG TCCCAAGTGA AAACAAGGAT
    CTGTATGAGT ACTCATGGGT CCAGGGCCCT TTGCATTCTA ATGAGACGGA CCGACTCCCC
    CGGTGTGTGC GTTCTACTGC ACGATTAGCC AGGGCCTTGT CTCCTGCCTT TGAACTGAGT
    CAGTGGAGCT CTACTGAATA CTCTACATGG ACTGAGAGCC GCTGGAAAGA TATCCGTGCC
    CGGATATTTC TCATCGCCAG CAAAGAGCTT GAGTTGATCA CCCTGACAGT GGGCTTCGGC
    ATCCTCATCT TCTCCCTCAT CGTCACCTAC TGCATCAATG CCAAAGCTGA TGTCCTTTTC
    ATTGCTCCCC GGGAGCCAGG AGCTGTGTCA TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08810
    Clone ID Related Accession (Same CDS sequence) NM_001290186.2 , NM_001290186.1
    Accession Version NM_001290186.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1716bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product nicastrin isoform 3 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF240468.1, AK299142.1, AL445230.11 and CN429672.1. Transcript Variant: This variant (3) lacks three alternate in-frame exons in the coding region compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.11658.1, SRR1803613.125282.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCTACGG CAGGGGGTGG CTCTGGGGCT GACCCGGGAA GTCGGGGTCT CCTTCGCCTT 
    CTGTCTTTCT GCGTCCTACT AGCAGGTTTG TGCAGGGGAA ACTCAGTGGA GAGGAAGATA
    TATATCCCCT TAAATAAAAC AGCTCCCTGT GTTCGCCTGC TCAACGCCAC TCATCAGATT
    GGCTGCCAGT CTTCAATTAG TGGAGACACA GGGGTTATCC ACGTAGTAGA GAAAGAGGAG
    GACCTACAGT GGGTATTGAC TGATGGCCCC AACCCCCCTT ACATGGTTCT GCTGGAGAGC
    AAGCATTTTA CCAGGGATTT AATGGAGAAG CTGAAAGGGA GAACCAGCCG AATTGCTGGT
    CTTGCAGTGT CCTTGACCAA GCCCAGTCCT GCCTCAGGCT TCTCTCCTAG TGTACAGTGC
    CCAAATGATG GGTTTGGTGT TTACTCCAAT TCCTATGGGC CAGAGTTTGC TCACTGCAGA
    GAAATACAGT GGAATTCGCT GGGCAATGGT TTGGCTTATG AAGACTTTAG TTTCCCCATC
    TTTCTTCTTG AAGATGAAAA TGAAACCAAA GTCATCAAGC AGGAAACTTT TGACTACATT
    GGCAGCTCGA GGATGGTCTA CGATATGGAG AAGGGCAAGT TTCCCGTGCA GTTAGAGAAT
    GTTGACTCAT TTGTGGAGCT GGGACAGGTG GCCTTAAGAA CTTCATTAGA GCTTTGGATG
    CACACAGATC CTGTTTCTCA GAAAAATGAG TCTGTACGGA ACCAGGTGGA GGATCTCCTG
    GCCACATTGG AGAAGAGTGG TGCTGGTGTC CCTGCTGTCA TCCTCAGGAG GCCAAATCAG
    TCCCAGCCTC TCCCACCATC TTCCCTGCAG CGATTTCTTC GAGCTCGAAA CATCTCTGGC
    GTTGTTCTGG CTGACCACTC TGGTGCCTTC CATAACAAAT ATTACCAGAG TATTTACGAC
    ACTGCTGAGA ACATTAATGT GAGCTATCCC GAATGGCTGA GCCCTGAAGA GGACCTGAAC
    TTTGTAACAG ACACTGCCAA GGCCCTGGCA GATGTGGCCA CGGTGCTGGG ACGTGCTCTG
    TATGAGCTTG CAGGAGGAAC CAACTTCAGC GACACAGTTC AGGCTGATCC CCAAACGGTT
    ACCCGCCTGC TCTATGGGTT CCTGATTAAA GCCAACAACT CATGGTTCCA GTCTATCCTC
    AGGCAGGACC TAAGGTCCTA CTTGGGTGAC GGGCCTCTTC AACATTACAT CGCTGTCTCC
    AGCCCCACCA ACACCACTTA TGTTGTACAG TATGCCTTGG CAAATTTGAC TGGCACAGTG
    GTCAACCTCA CCCGAGAGCA GTGCCAGGAT CCAAGTAAAG TCCCAAGTGA AAACAAGGAT
    CTGTATGAGT ACTCATGGGT CCAGGGCCCT TTGCATTCTA ATGAGACGGA CCGACTCCCC
    CGGTGTGTGC GTTCTACTGC ACGATTAGCC AGGGCCTTGT CTCCTGCCTT TGAACTGAGT
    CAGTGGAGCT CTACTGAATA CTCTACATGG ACTGAGAGCC GCTGGAAAGA TATCCGTGCC
    CGGATATTTC TCATCGCCAG CAAAGAGCTT GAGTTGATCA CCCTGACAGT GGGCTTCGGC
    ATCCTCATCT TCTCCCTCAT CGTCACCTAC TGCATCAATG CCAAAGCTGA TGTCCTTTTC
    ATTGCTCCCC GGGAGCCAGG AGCTGTGTCA TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001277115.1
    CDS125..1840
    Translation

    Target ORF information:

    RefSeq Version NM_001290186.1
    Organism Homo sapiens(human)
    Definition Homo sapiens nicastrin (NCSTN), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001290186.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCTACGG CAGGGGGTGG CTCTGGGGCT GACCCGGGAA GTCGGGGTCT CCTTCGCCTT 
    CTGTCTTTCT GCGTCCTACT AGCAGGTTTG TGCAGGGGAA ACTCAGTGGA GAGGAAGATA
    TATATCCCCT TAAATAAAAC AGCTCCCTGT GTTCGCCTGC TCAACGCCAC TCATCAGATT
    GGCTGCCAGT CTTCAATTAG TGGAGACACA GGGGTTATCC ACGTAGTAGA GAAAGAGGAG
    GACCTACAGT GGGTATTGAC TGATGGCCCC AACCCCCCTT ACATGGTTCT GCTGGAGAGC
    AAGCATTTTA CCAGGGATTT AATGGAGAAG CTGAAAGGGA GAACCAGCCG AATTGCTGGT
    CTTGCAGTGT CCTTGACCAA GCCCAGTCCT GCCTCAGGCT TCTCTCCTAG TGTACAGTGC
    CCAAATGATG GGTTTGGTGT TTACTCCAAT TCCTATGGGC CAGAGTTTGC TCACTGCAGA
    GAAATACAGT GGAATTCGCT GGGCAATGGT TTGGCTTATG AAGACTTTAG TTTCCCCATC
    TTTCTTCTTG AAGATGAAAA TGAAACCAAA GTCATCAAGC AGGAAACTTT TGACTACATT
    GGCAGCTCGA GGATGGTCTA CGATATGGAG AAGGGCAAGT TTCCCGTGCA GTTAGAGAAT
    GTTGACTCAT TTGTGGAGCT GGGACAGGTG GCCTTAAGAA CTTCATTAGA GCTTTGGATG
    CACACAGATC CTGTTTCTCA GAAAAATGAG TCTGTACGGA ACCAGGTGGA GGATCTCCTG
    GCCACATTGG AGAAGAGTGG TGCTGGTGTC CCTGCTGTCA TCCTCAGGAG GCCAAATCAG
    TCCCAGCCTC TCCCACCATC TTCCCTGCAG CGATTTCTTC GAGCTCGAAA CATCTCTGGC
    GTTGTTCTGG CTGACCACTC TGGTGCCTTC CATAACAAAT ATTACCAGAG TATTTACGAC
    ACTGCTGAGA ACATTAATGT GAGCTATCCC GAATGGCTGA GCCCTGAAGA GGACCTGAAC
    TTTGTAACAG ACACTGCCAA GGCCCTGGCA GATGTGGCCA CGGTGCTGGG ACGTGCTCTG
    TATGAGCTTG CAGGAGGAAC CAACTTCAGC GACACAGTTC AGGCTGATCC CCAAACGGTT
    ACCCGCCTGC TCTATGGGTT CCTGATTAAA GCCAACAACT CATGGTTCCA GTCTATCCTC
    AGGCAGGACC TAAGGTCCTA CTTGGGTGAC GGGCCTCTTC AACATTACAT CGCTGTCTCC
    AGCCCCACCA ACACCACTTA TGTTGTACAG TATGCCTTGG CAAATTTGAC TGGCACAGTG
    GTCAACCTCA CCCGAGAGCA GTGCCAGGAT CCAAGTAAAG TCCCAAGTGA AAACAAGGAT
    CTGTATGAGT ACTCATGGGT CCAGGGCCCT TTGCATTCTA ATGAGACGGA CCGACTCCCC
    CGGTGTGTGC GTTCTACTGC ACGATTAGCC AGGGCCTTGT CTCCTGCCTT TGAACTGAGT
    CAGTGGAGCT CTACTGAATA CTCTACATGG ACTGAGAGCC GCTGGAAAGA TATCCGTGCC
    CGGATATTTC TCATCGCCAG CAAAGAGCTT GAGTTGATCA CCCTGACAGT GGGCTTCGGC
    ATCCTCATCT TCTCCCTCAT CGTCACCTAC TGCATCAATG CCAAAGCTGA TGTCCTTTTC
    ATTGCTCCCC GGGAGCCAGG AGCTGTGTCA TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu66123
    Clone ID Related Accession (Same CDS sequence) NM_001349729.1 , NM_001349729.2
    Accession Version NM_001349729.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1917bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product nicastrin isoform 4 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF240468.1, AL445230.11 and H15133.1. On Apr 7, 2017 this sequence version replaced XM_011509363.2. Transcript Variant: This variant (5) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. It encodes isoform 5, which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.154849.1, SRR1660807.263787.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCTACGG CAGGGGGTGG CTCTGGGGCT GACCCGGGAA GTCGGGGTCT CCTTCGCCTT 
    CTGTCTTTCT GCGTCCTACT AGCAGGTTTG TGCAGGGGAA ACTCAGTGGA GAGGAAGATA
    TATATCCCCT TAAATAAAAC AGCTCCCTGT GTTCGCCTGC TCAACGCCAC TCATCAGATT
    GGCTGCCAGT CTTCAATTAG TGGAGACACA GGGGTTATCC ACGTAGTAGA GAAAGAGGAG
    GACCTACAGT GGGTATTGAC TGATGGCCCC AACCCCCCTT ACATGGTTCT GCTGGAGAGC
    AAGCATTTTA CCAGGGATTT AATGGAGAAG CTGAAAGGGA GAACCAGCCG AATTGCTGGT
    CTTGCAGTGT CCTTGACCAA GCCCAGTCCT GCCTCAGGCT TCTCTCCTAG TGTACAGTGC
    CCAAATGATG GGTTTGGTGT TTACTCCAAT TCCTATGGGC CAGAGTTTGC TCACTGCAGA
    GAAATACAGT GGAATTCGCT GGGCAATGGT TTGGCTTATG AAGACTTTAG TTTCCCCATC
    TTTCTTCTTG AAGATGAAAA TGAAACCAAA GTCATCAAGC AGTGCTATCA AGATCACAAC
    CTGAGTCAGA ATGGCTCAGC ACCAACCTTC CCACTATGTG CCATGCAGCT CTTTTCACAC
    ATGCATGCTG TCATCAGCAC TGCCACCTGC ATGCGGCGCA GCTCCATCCA AAGCACCTTC
    AGCATCAACC CAGAAATCGT CTGTGACCCC CTGTCTGATT ACAATGTGTG GAGCATGCTA
    AAGCCTATAA ATACAACTGG GACATTAAAG CCTGACGACA GGGTTGTGGT TGCTGCCACC
    CGGCTGGATA GTCGTTCCTT TTTCTGGAAT GTGGCCCCAG GGGCTGAAAG CGCAGTGGCT
    TCCTTTGTCA CCCAGCTGGC TGCTGCTGAA GCTTTGCAAA AGGCACCTGA TGTGACCACC
    CTGCCCCGCA ATGTCATGTT TGTCTTCTTT CAAGGGGAAA CTTTTGACTA CATTGGCAGC
    TCGAGGATGG TCTACGATAT GGAGAAGGGC AAGTTTCCCG TGCAGTTAGA GAATGTTGAC
    TCATTTGTGG AGCTGGGACA GGTGGCCTTA AGAACTTCAT TAGAGCTTTG GATGCACACA
    GATCCTGTTT CTCAGAAAAA TGAGTCTGTA CGGAACCAGG TGGAGGATCT CCTGGCCACA
    TTGGAGAAGA GTGGTGCTGG TGTCCCTGCT GTCATCCTCA GGAGGCCAAA TCAGTCCCAG
    CCTCTCCCAC CATCTTCCCT GCAGCGATTT CTTCGAGCTC GAAACATCTC TGGCGTTGTT
    CTGGCTGACC ACTCTGGTGC CTTCCATAAC AAATATTACC AGAGTATTTA CGACACTGCT
    GAGAACATTA ATGTGAGCTA TCCCGAATGG CTGAGCCCTG AAGAGGACCT GAACTTTGTA
    ACAGACACTG CCAAGGCCCT GGCAGATGTG GCCACGGTGC TGGGACGTGC TCTGTATGAG
    CTTGCAGGAG GAACCAACTT CAGCGACACA GTTCAGGCTG ATCCCCAAAC GGTTACCCGC
    CTGCTCTATG GGTTCCTGAT TAAAGCCAAC AACTCATGGT TCCAGTCTAT CCTCAGGCAG
    GACCTAAGGT CCTACTTGGG TGACGGGCCT CTTCAACATT ACATCGCTGT CTCCAGCCCC
    ACCAACACCA CTTATGTTGT ACAGTATGCC TTGGCAAATT TGACTGGCAC AGTGGTCAAC
    CTCACCCGAG AGCAGTGCCA GGATCCAAGT AAAGTCCCAA GTGAAAACAA GGATTTGATC
    ACCCTGACAG TGGGCTTCGG CATCCTCATC TTCTCCCTCA TCGTCACCTA CTGCATCAAT
    GCCAAAGCTG ATGTCCTTTT CATTGCTCCC CGGGAGCCAG GAGCTGTGTC ATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336658.1
    CDS125..2041
    Translation

    Target ORF information:

    RefSeq Version NM_001349729.1
    Organism Homo sapiens(human)
    Definition Homo sapiens nicastrin (NCSTN), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349729.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCTACGG CAGGGGGTGG CTCTGGGGCT GACCCGGGAA GTCGGGGTCT CCTTCGCCTT 
    CTGTCTTTCT GCGTCCTACT AGCAGGTTTG TGCAGGGGAA ACTCAGTGGA GAGGAAGATA
    TATATCCCCT TAAATAAAAC AGCTCCCTGT GTTCGCCTGC TCAACGCCAC TCATCAGATT
    GGCTGCCAGT CTTCAATTAG TGGAGACACA GGGGTTATCC ACGTAGTAGA GAAAGAGGAG
    GACCTACAGT GGGTATTGAC TGATGGCCCC AACCCCCCTT ACATGGTTCT GCTGGAGAGC
    AAGCATTTTA CCAGGGATTT AATGGAGAAG CTGAAAGGGA GAACCAGCCG AATTGCTGGT
    CTTGCAGTGT CCTTGACCAA GCCCAGTCCT GCCTCAGGCT TCTCTCCTAG TGTACAGTGC
    CCAAATGATG GGTTTGGTGT TTACTCCAAT TCCTATGGGC CAGAGTTTGC TCACTGCAGA
    GAAATACAGT GGAATTCGCT GGGCAATGGT TTGGCTTATG AAGACTTTAG TTTCCCCATC
    TTTCTTCTTG AAGATGAAAA TGAAACCAAA GTCATCAAGC AGTGCTATCA AGATCACAAC
    CTGAGTCAGA ATGGCTCAGC ACCAACCTTC CCACTATGTG CCATGCAGCT CTTTTCACAC
    ATGCATGCTG TCATCAGCAC TGCCACCTGC ATGCGGCGCA GCTCCATCCA AAGCACCTTC
    AGCATCAACC CAGAAATCGT CTGTGACCCC CTGTCTGATT ACAATGTGTG GAGCATGCTA
    AAGCCTATAA ATACAACTGG GACATTAAAG CCTGACGACA GGGTTGTGGT TGCTGCCACC
    CGGCTGGATA GTCGTTCCTT TTTCTGGAAT GTGGCCCCAG GGGCTGAAAG CGCAGTGGCT
    TCCTTTGTCA CCCAGCTGGC TGCTGCTGAA GCTTTGCAAA AGGCACCTGA TGTGACCACC
    CTGCCCCGCA ATGTCATGTT TGTCTTCTTT CAAGGGGAAA CTTTTGACTA CATTGGCAGC
    TCGAGGATGG TCTACGATAT GGAGAAGGGC AAGTTTCCCG TGCAGTTAGA GAATGTTGAC
    TCATTTGTGG AGCTGGGACA GGTGGCCTTA AGAACTTCAT TAGAGCTTTG GATGCACACA
    GATCCTGTTT CTCAGAAAAA TGAGTCTGTA CGGAACCAGG TGGAGGATCT CCTGGCCACA
    TTGGAGAAGA GTGGTGCTGG TGTCCCTGCT GTCATCCTCA GGAGGCCAAA TCAGTCCCAG
    CCTCTCCCAC CATCTTCCCT GCAGCGATTT CTTCGAGCTC GAAACATCTC TGGCGTTGTT
    CTGGCTGACC ACTCTGGTGC CTTCCATAAC AAATATTACC AGAGTATTTA CGACACTGCT
    GAGAACATTA ATGTGAGCTA TCCCGAATGG CTGAGCCCTG AAGAGGACCT GAACTTTGTA
    ACAGACACTG CCAAGGCCCT GGCAGATGTG GCCACGGTGC TGGGACGTGC TCTGTATGAG
    CTTGCAGGAG GAACCAACTT CAGCGACACA GTTCAGGCTG ATCCCCAAAC GGTTACCCGC
    CTGCTCTATG GGTTCCTGAT TAAAGCCAAC AACTCATGGT TCCAGTCTAT CCTCAGGCAG
    GACCTAAGGT CCTACTTGGG TGACGGGCCT CTTCAACATT ACATCGCTGT CTCCAGCCCC
    ACCAACACCA CTTATGTTGT ACAGTATGCC TTGGCAAATT TGACTGGCAC AGTGGTCAAC
    CTCACCCGAG AGCAGTGCCA GGATCCAAGT AAAGTCCCAA GTGAAAACAA GGATTTGATC
    ACCCTGACAG TGGGCTTCGG CATCCTCATC TTCTCCCTCA TCGTCACCTA CTGCATCAAT
    GCCAAAGCTG ATGTCCTTTT CATTGCTCCC CGGGAGCCAG GAGCTGTGTC ATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu66123
    Clone ID Related Accession (Same CDS sequence) NM_001349729.1 , NM_001349729.2
    Accession Version NM_001349729.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1917bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product nicastrin isoform 4 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL445230.11 and H15133.1. On May 31, 2019 this sequence version replaced NM_001349729.1. Transcript Variant: This variant (5) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. It encodes isoform 5, which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.179413.1, SRR1660807.263787.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCTACGG CAGGGGGTGG CTCTGGGGCT GACCCGGGAA GTCGGGGTCT CCTTCGCCTT 
    CTGTCTTTCT GCGTCCTACT AGCAGGTTTG TGCAGGGGAA ACTCAGTGGA GAGGAAGATA
    TATATCCCCT TAAATAAAAC AGCTCCCTGT GTTCGCCTGC TCAACGCCAC TCATCAGATT
    GGCTGCCAGT CTTCAATTAG TGGAGACACA GGGGTTATCC ACGTAGTAGA GAAAGAGGAG
    GACCTACAGT GGGTATTGAC TGATGGCCCC AACCCCCCTT ACATGGTTCT GCTGGAGAGC
    AAGCATTTTA CCAGGGATTT AATGGAGAAG CTGAAAGGGA GAACCAGCCG AATTGCTGGT
    CTTGCAGTGT CCTTGACCAA GCCCAGTCCT GCCTCAGGCT TCTCTCCTAG TGTACAGTGC
    CCAAATGATG GGTTTGGTGT TTACTCCAAT TCCTATGGGC CAGAGTTTGC TCACTGCAGA
    GAAATACAGT GGAATTCGCT GGGCAATGGT TTGGCTTATG AAGACTTTAG TTTCCCCATC
    TTTCTTCTTG AAGATGAAAA TGAAACCAAA GTCATCAAGC AGTGCTATCA AGATCACAAC
    CTGAGTCAGA ATGGCTCAGC ACCAACCTTC CCACTATGTG CCATGCAGCT CTTTTCACAC
    ATGCATGCTG TCATCAGCAC TGCCACCTGC ATGCGGCGCA GCTCCATCCA AAGCACCTTC
    AGCATCAACC CAGAAATCGT CTGTGACCCC CTGTCTGATT ACAATGTGTG GAGCATGCTA
    AAGCCTATAA ATACAACTGG GACATTAAAG CCTGACGACA GGGTTGTGGT TGCTGCCACC
    CGGCTGGATA GTCGTTCCTT TTTCTGGAAT GTGGCCCCAG GGGCTGAAAG CGCAGTGGCT
    TCCTTTGTCA CCCAGCTGGC TGCTGCTGAA GCTTTGCAAA AGGCACCTGA TGTGACCACC
    CTGCCCCGCA ATGTCATGTT TGTCTTCTTT CAAGGGGAAA CTTTTGACTA CATTGGCAGC
    TCGAGGATGG TCTACGATAT GGAGAAGGGC AAGTTTCCCG TGCAGTTAGA GAATGTTGAC
    TCATTTGTGG AGCTGGGACA GGTGGCCTTA AGAACTTCAT TAGAGCTTTG GATGCACACA
    GATCCTGTTT CTCAGAAAAA TGAGTCTGTA CGGAACCAGG TGGAGGATCT CCTGGCCACA
    TTGGAGAAGA GTGGTGCTGG TGTCCCTGCT GTCATCCTCA GGAGGCCAAA TCAGTCCCAG
    CCTCTCCCAC CATCTTCCCT GCAGCGATTT CTTCGAGCTC GAAACATCTC TGGCGTTGTT
    CTGGCTGACC ACTCTGGTGC CTTCCATAAC AAATATTACC AGAGTATTTA CGACACTGCT
    GAGAACATTA ATGTGAGCTA TCCCGAATGG CTGAGCCCTG AAGAGGACCT GAACTTTGTA
    ACAGACACTG CCAAGGCCCT GGCAGATGTG GCCACGGTGC TGGGACGTGC TCTGTATGAG
    CTTGCAGGAG GAACCAACTT CAGCGACACA GTTCAGGCTG ATCCCCAAAC GGTTACCCGC
    CTGCTCTATG GGTTCCTGAT TAAAGCCAAC AACTCATGGT TCCAGTCTAT CCTCAGGCAG
    GACCTAAGGT CCTACTTGGG TGACGGGCCT CTTCAACATT ACATCGCTGT CTCCAGCCCC
    ACCAACACCA CTTATGTTGT ACAGTATGCC TTGGCAAATT TGACTGGCAC AGTGGTCAAC
    CTCACCCGAG AGCAGTGCCA GGATCCAAGT AAAGTCCCAA GTGAAAACAA GGATTTGATC
    ACCCTGACAG TGGGCTTCGG CATCCTCATC TTCTCCCTCA TCGTCACCTA CTGCATCAAT
    GCCAAAGCTG ATGTCCTTTT CATTGCTCCC CGGGAGCCAG GAGCTGTGTC ATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336658.1
    CDS15..1931
    Translation

    Target ORF information:

    RefSeq Version NM_001349729.2
    Organism Homo sapiens(human)
    Definition Homo sapiens nicastrin (NCSTN), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349729.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCTACGG CAGGGGGTGG CTCTGGGGCT GACCCGGGAA GTCGGGGTCT CCTTCGCCTT 
    CTGTCTTTCT GCGTCCTACT AGCAGGTTTG TGCAGGGGAA ACTCAGTGGA GAGGAAGATA
    TATATCCCCT TAAATAAAAC AGCTCCCTGT GTTCGCCTGC TCAACGCCAC TCATCAGATT
    GGCTGCCAGT CTTCAATTAG TGGAGACACA GGGGTTATCC ACGTAGTAGA GAAAGAGGAG
    GACCTACAGT GGGTATTGAC TGATGGCCCC AACCCCCCTT ACATGGTTCT GCTGGAGAGC
    AAGCATTTTA CCAGGGATTT AATGGAGAAG CTGAAAGGGA GAACCAGCCG AATTGCTGGT
    CTTGCAGTGT CCTTGACCAA GCCCAGTCCT GCCTCAGGCT TCTCTCCTAG TGTACAGTGC
    CCAAATGATG GGTTTGGTGT TTACTCCAAT TCCTATGGGC CAGAGTTTGC TCACTGCAGA
    GAAATACAGT GGAATTCGCT GGGCAATGGT TTGGCTTATG AAGACTTTAG TTTCCCCATC
    TTTCTTCTTG AAGATGAAAA TGAAACCAAA GTCATCAAGC AGTGCTATCA AGATCACAAC
    CTGAGTCAGA ATGGCTCAGC ACCAACCTTC CCACTATGTG CCATGCAGCT CTTTTCACAC
    ATGCATGCTG TCATCAGCAC TGCCACCTGC ATGCGGCGCA GCTCCATCCA AAGCACCTTC
    AGCATCAACC CAGAAATCGT CTGTGACCCC CTGTCTGATT ACAATGTGTG GAGCATGCTA
    AAGCCTATAA ATACAACTGG GACATTAAAG CCTGACGACA GGGTTGTGGT TGCTGCCACC
    CGGCTGGATA GTCGTTCCTT TTTCTGGAAT GTGGCCCCAG GGGCTGAAAG CGCAGTGGCT
    TCCTTTGTCA CCCAGCTGGC TGCTGCTGAA GCTTTGCAAA AGGCACCTGA TGTGACCACC
    CTGCCCCGCA ATGTCATGTT TGTCTTCTTT CAAGGGGAAA CTTTTGACTA CATTGGCAGC
    TCGAGGATGG TCTACGATAT GGAGAAGGGC AAGTTTCCCG TGCAGTTAGA GAATGTTGAC
    TCATTTGTGG AGCTGGGACA GGTGGCCTTA AGAACTTCAT TAGAGCTTTG GATGCACACA
    GATCCTGTTT CTCAGAAAAA TGAGTCTGTA CGGAACCAGG TGGAGGATCT CCTGGCCACA
    TTGGAGAAGA GTGGTGCTGG TGTCCCTGCT GTCATCCTCA GGAGGCCAAA TCAGTCCCAG
    CCTCTCCCAC CATCTTCCCT GCAGCGATTT CTTCGAGCTC GAAACATCTC TGGCGTTGTT
    CTGGCTGACC ACTCTGGTGC CTTCCATAAC AAATATTACC AGAGTATTTA CGACACTGCT
    GAGAACATTA ATGTGAGCTA TCCCGAATGG CTGAGCCCTG AAGAGGACCT GAACTTTGTA
    ACAGACACTG CCAAGGCCCT GGCAGATGTG GCCACGGTGC TGGGACGTGC TCTGTATGAG
    CTTGCAGGAG GAACCAACTT CAGCGACACA GTTCAGGCTG ATCCCCAAAC GGTTACCCGC
    CTGCTCTATG GGTTCCTGAT TAAAGCCAAC AACTCATGGT TCCAGTCTAT CCTCAGGCAG
    GACCTAAGGT CCTACTTGGG TGACGGGCCT CTTCAACATT ACATCGCTGT CTCCAGCCCC
    ACCAACACCA CTTATGTTGT ACAGTATGCC TTGGCAAATT TGACTGGCAC AGTGGTCAAC
    CTCACCCGAG AGCAGTGCCA GGATCCAAGT AAAGTCCCAA GTGAAAACAA GGATTTGATC
    ACCCTGACAG TGGGCTTCGG CATCCTCATC TTCTCCCTCA TCGTCACCTA CTGCATCAAT
    GCCAAAGCTG ATGTCCTTTT CATTGCTCCC CGGGAGCCAG GAGCTGTGTC ATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34278
    Clone ID Related Accession (Same CDS sequence) XM_005245053.4 , XM_005245053.5
    Accession Version XM_005245053.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product nicastrin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005245053.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGCTACGG CAGGGGGTGG CTCTGGGGCT GACCCGGGAA GTCGGGGTCT CCTTCGCCTT 
    CTGTCTTTCT GCGTCCTACT AGCAGGTTTG TGCAGGGGAA ACTCAGTGGA GAGGAAGATA
    TATATCCCCT TAAATAAAAC AGCTCCCTGT GTTCGCCTGC TCAACGCCAC TCATCAGATT
    GGCTGCCAGT CTTCAATTAG TGGAGACACA GGGGTTATCC ACGTAGTAGA GAAAGAGGAG
    GACCTACAGT GGGTATTGAC TGATGGCCCC AACCCCCCTT ACATGGTTCT GCTGGAGAGC
    AAGCATTTTA CCAGGGATTT AATGGAGAAG CTGAAAGGGA GAACCAGCCG AATTGCTGGT
    CTTGCAGTGT CCTTGACCAA GCCCAGTCCT GCCTCAGGCT TCTCTCCTAG TGTACAGTGC
    CCAAATGATG GGTTTGGTGT TTACTCCAAT TCCTATGGGC CAGAGTTTGC TCACTGCAGA
    GAAATACAGT GGAATTCGCT GGGCAATGGT TTGGCTTATG AAGACTTTAG TTTCCCCATC
    TTTCTTCTTG AAGATGAAAA TGAAACCAAA GTCATCAAGC AGTGCTATCA AGATCACAAC
    CTGAGTCAGA ATGGCTCAGC ACCAACCTTC CCACTATGTG CCATGCAGCT CTTTTCACAC
    ATGCATGCTG TCATCAGCAC TGCCACCTGC ATGCGGCGCA GCTCCATCCA AAGCACCTTC
    AGCATCAACC CAGAAATCGT CTGTGACCCC CTGTCTGATT ACAATGTGTG GAGCATGCTA
    AAGCCTATAA ATACAACTGG GACATTAAAG CCTGACGACA GGGTTGTGGT TGCTGCCACC
    CGGCTGGATA GTCGTTCCTT TTTCTGGAAT GTGGCCCCAG GGGCTGAAAG CGCAGTGGCT
    TCCTTTGTCA CCCAGCTGGC TGCTGCTGAA GCTTTGCAAA AGGCACCTGA TGTGACCACC
    CTGCCCCGCA ATGTCATGTT TGTCTTCTTT CAAGGGGAAA CTTTTGACTA CATTGGCAGC
    TCGAGGATGG TCTACGATAT GGAGAAGGGC AAGTTTCCCG TGCAGTTAGA GAATGTTGAC
    TCATTTGTGG AGCTGGGACA GGTGGCCTTA AGAACTTCAT TAGAGCTTTG GATGCACACA
    GATCCTGTTT CTCAGAAAAA TGAGTCTGTA CGGAACCAGG GCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005245110.1
    CDS342..1529
    Translation

    Target ORF information:

    RefSeq Version XM_005245053.4
    Organism Homo sapiens(human)
    Definition Homo sapiens nicastrin (NCSTN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005245053.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGCTACGG CAGGGGGTGG CTCTGGGGCT GACCCGGGAA GTCGGGGTCT CCTTCGCCTT 
    CTGTCTTTCT GCGTCCTACT AGCAGGTTTG TGCAGGGGAA ACTCAGTGGA GAGGAAGATA
    TATATCCCCT TAAATAAAAC AGCTCCCTGT GTTCGCCTGC TCAACGCCAC TCATCAGATT
    GGCTGCCAGT CTTCAATTAG TGGAGACACA GGGGTTATCC ACGTAGTAGA GAAAGAGGAG
    GACCTACAGT GGGTATTGAC TGATGGCCCC AACCCCCCTT ACATGGTTCT GCTGGAGAGC
    AAGCATTTTA CCAGGGATTT AATGGAGAAG CTGAAAGGGA GAACCAGCCG AATTGCTGGT
    CTTGCAGTGT CCTTGACCAA GCCCAGTCCT GCCTCAGGCT TCTCTCCTAG TGTACAGTGC
    CCAAATGATG GGTTTGGTGT TTACTCCAAT TCCTATGGGC CAGAGTTTGC TCACTGCAGA
    GAAATACAGT GGAATTCGCT GGGCAATGGT TTGGCTTATG AAGACTTTAG TTTCCCCATC
    TTTCTTCTTG AAGATGAAAA TGAAACCAAA GTCATCAAGC AGTGCTATCA AGATCACAAC
    CTGAGTCAGA ATGGCTCAGC ACCAACCTTC CCACTATGTG CCATGCAGCT CTTTTCACAC
    ATGCATGCTG TCATCAGCAC TGCCACCTGC ATGCGGCGCA GCTCCATCCA AAGCACCTTC
    AGCATCAACC CAGAAATCGT CTGTGACCCC CTGTCTGATT ACAATGTGTG GAGCATGCTA
    AAGCCTATAA ATACAACTGG GACATTAAAG CCTGACGACA GGGTTGTGGT TGCTGCCACC
    CGGCTGGATA GTCGTTCCTT TTTCTGGAAT GTGGCCCCAG GGGCTGAAAG CGCAGTGGCT
    TCCTTTGTCA CCCAGCTGGC TGCTGCTGAA GCTTTGCAAA AGGCACCTGA TGTGACCACC
    CTGCCCCGCA ATGTCATGTT TGTCTTCTTT CAAGGGGAAA CTTTTGACTA CATTGGCAGC
    TCGAGGATGG TCTACGATAT GGAGAAGGGC AAGTTTCCCG TGCAGTTAGA GAATGTTGAC
    TCATTTGTGG AGCTGGGACA GGTGGCCTTA AGAACTTCAT TAGAGCTTTG GATGCACACA
    GATCCTGTTT CTCAGAAAAA TGAGTCTGTA CGGAACCAGG GCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34278
    Clone ID Related Accession (Same CDS sequence) XM_005245053.4 , XM_005245053.5
    Accession Version XM_005245053.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product nicastrin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005245053.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGCTACGG CAGGGGGTGG CTCTGGGGCT GACCCGGGAA GTCGGGGTCT CCTTCGCCTT 
    CTGTCTTTCT GCGTCCTACT AGCAGGTTTG TGCAGGGGAA ACTCAGTGGA GAGGAAGATA
    TATATCCCCT TAAATAAAAC AGCTCCCTGT GTTCGCCTGC TCAACGCCAC TCATCAGATT
    GGCTGCCAGT CTTCAATTAG TGGAGACACA GGGGTTATCC ACGTAGTAGA GAAAGAGGAG
    GACCTACAGT GGGTATTGAC TGATGGCCCC AACCCCCCTT ACATGGTTCT GCTGGAGAGC
    AAGCATTTTA CCAGGGATTT AATGGAGAAG CTGAAAGGGA GAACCAGCCG AATTGCTGGT
    CTTGCAGTGT CCTTGACCAA GCCCAGTCCT GCCTCAGGCT TCTCTCCTAG TGTACAGTGC
    CCAAATGATG GGTTTGGTGT TTACTCCAAT TCCTATGGGC CAGAGTTTGC TCACTGCAGA
    GAAATACAGT GGAATTCGCT GGGCAATGGT TTGGCTTATG AAGACTTTAG TTTCCCCATC
    TTTCTTCTTG AAGATGAAAA TGAAACCAAA GTCATCAAGC AGTGCTATCA AGATCACAAC
    CTGAGTCAGA ATGGCTCAGC ACCAACCTTC CCACTATGTG CCATGCAGCT CTTTTCACAC
    ATGCATGCTG TCATCAGCAC TGCCACCTGC ATGCGGCGCA GCTCCATCCA AAGCACCTTC
    AGCATCAACC CAGAAATCGT CTGTGACCCC CTGTCTGATT ACAATGTGTG GAGCATGCTA
    AAGCCTATAA ATACAACTGG GACATTAAAG CCTGACGACA GGGTTGTGGT TGCTGCCACC
    CGGCTGGATA GTCGTTCCTT TTTCTGGAAT GTGGCCCCAG GGGCTGAAAG CGCAGTGGCT
    TCCTTTGTCA CCCAGCTGGC TGCTGCTGAA GCTTTGCAAA AGGCACCTGA TGTGACCACC
    CTGCCCCGCA ATGTCATGTT TGTCTTCTTT CAAGGGGAAA CTTTTGACTA CATTGGCAGC
    TCGAGGATGG TCTACGATAT GGAGAAGGGC AAGTTTCCCG TGCAGTTAGA GAATGTTGAC
    TCATTTGTGG AGCTGGGACA GGTGGCCTTA AGAACTTCAT TAGAGCTTTG GATGCACACA
    GATCCTGTTT CTCAGAAAAA TGAGTCTGTA CGGAACCAGG GCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005245110.1
    CDS70..1257
    Translation

    Target ORF information:

    RefSeq Version XM_005245053.5
    Organism Homo sapiens(human)
    Definition Homo sapiens nicastrin (NCSTN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005245053.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGCTACGG CAGGGGGTGG CTCTGGGGCT GACCCGGGAA GTCGGGGTCT CCTTCGCCTT 
    CTGTCTTTCT GCGTCCTACT AGCAGGTTTG TGCAGGGGAA ACTCAGTGGA GAGGAAGATA
    TATATCCCCT TAAATAAAAC AGCTCCCTGT GTTCGCCTGC TCAACGCCAC TCATCAGATT
    GGCTGCCAGT CTTCAATTAG TGGAGACACA GGGGTTATCC ACGTAGTAGA GAAAGAGGAG
    GACCTACAGT GGGTATTGAC TGATGGCCCC AACCCCCCTT ACATGGTTCT GCTGGAGAGC
    AAGCATTTTA CCAGGGATTT AATGGAGAAG CTGAAAGGGA GAACCAGCCG AATTGCTGGT
    CTTGCAGTGT CCTTGACCAA GCCCAGTCCT GCCTCAGGCT TCTCTCCTAG TGTACAGTGC
    CCAAATGATG GGTTTGGTGT TTACTCCAAT TCCTATGGGC CAGAGTTTGC TCACTGCAGA
    GAAATACAGT GGAATTCGCT GGGCAATGGT TTGGCTTATG AAGACTTTAG TTTCCCCATC
    TTTCTTCTTG AAGATGAAAA TGAAACCAAA GTCATCAAGC AGTGCTATCA AGATCACAAC
    CTGAGTCAGA ATGGCTCAGC ACCAACCTTC CCACTATGTG CCATGCAGCT CTTTTCACAC
    ATGCATGCTG TCATCAGCAC TGCCACCTGC ATGCGGCGCA GCTCCATCCA AAGCACCTTC
    AGCATCAACC CAGAAATCGT CTGTGACCCC CTGTCTGATT ACAATGTGTG GAGCATGCTA
    AAGCCTATAA ATACAACTGG GACATTAAAG CCTGACGACA GGGTTGTGGT TGCTGCCACC
    CGGCTGGATA GTCGTTCCTT TTTCTGGAAT GTGGCCCCAG GGGCTGAAAG CGCAGTGGCT
    TCCTTTGTCA CCCAGCTGGC TGCTGCTGAA GCTTTGCAAA AGGCACCTGA TGTGACCACC
    CTGCCCCGCA ATGTCATGTT TGTCTTCTTT CAAGGGGAAA CTTTTGACTA CATTGGCAGC
    TCGAGGATGG TCTACGATAT GGAGAAGGGC AAGTTTCCCG TGCAGTTAGA GAATGTTGAC
    TCATTTGTGG AGCTGGGACA GGTGGCCTTA AGAACTTCAT TAGAGCTTTG GATGCACACA
    GATCCTGTTT CTCAGAAAAA TGAGTCTGTA CGGAACCAGG GCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08813
    Clone ID Related Accession (Same CDS sequence) NM_001290184.2 , NM_001290184.1
    Accession Version NM_001290184.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product nicastrin isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC047621.1, AL445230.11 and CN429672.1. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_001290184.1. Transcript Variant: This variant (2) has a different 5' structure resulting in translation initiation at an alternate AUG compared to variant 1; the 5'-most AUG, as used in variant 1, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the alternate AUG resulting in a shorter isoform (2) with a distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC047621.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820687 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACTTTA ATCTCATTTT AGAAAGTTTG TGCAGGGGAA ACTCAGTGGA GAGGAAGATA 
    TATATCCCCT TAAATAAAAC AGCTCCCTGT GTTCGCCTGC TCAACGCCAC TCATCAGATT
    GGCTGCCAGT CTTCAATTAG TGGAGACACA GGGGTTATCC ACGTAGTAGA GAAAGAGGAG
    GACCTACAGT GGGTATTGAC TGATGGCCCC AACCCCCCTT ACATGGTTCT GCTGGAGAGC
    AAGCATTTTA CCAGGGATTT AATGGAGAAG CTGAAAGGGA GAACCAGCCG AATTGCTGGT
    CTTGCAGTGT CCTTGACCAA GCCCAGTCCT GCCTCAGGCT TCTCTCCTAG TGTACAGTGC
    CCAAATGATG GGTTTGGTGT TTACTCCAAT TCCTATGGGC CAGAGTTTGC TCACTGCAGA
    GAAATACAGT GGAATTCGCT GGGCAATGGT TTGGCTTATG AAGACTTTAG TTTCCCCATC
    TTTCTTCTTG AAGATGAAAA TGAAACCAAA GTCATCAAGC AGTGCTATCA AGATCACAAC
    CTGAGTCAGA ATGGCTCAGC ACCAACCTTC CCACTATGTG CCATGCAGCT CTTTTCACAC
    ATGCATGCTG TCATCAGCAC TGCCACCTGC ATGCGGCGCA GCTCCATCCA AAGCACCTTC
    AGCATCAACC CAGAAATCGT CTGTGACCCC CTGTCTGATT ACAATGTGTG GAGCATGCTA
    AAGCCTATAA ATACAACTGG GACATTAAAG CCTGACGACA GGGTTGTGGT TGCTGCCACC
    CGGCTGGATA GTCGTTCCTT TTTCTGGAAT GTGGCCCCAG GGGCTGAAAG CGCAGTGGCT
    TCCTTTGTCA CCCAGCTGGC TGCTGCTGAA GCTTTGCAAA AGGCACCTGA TGTGACCACC
    CTGCCCCGCA ATGTCATGTT TGTCTTCTTT CAAGGGGAAA CTTTTGACTA CATTGGCAGC
    TCGAGGATGG TCTACGATAT GGAGAAGGGC AAGTTTCCCG TGCAGTTAGA GAATGTTGAC
    TCATTTGTGG AGCTGGGACA GGTGGCCTTA AGAACTTCAT TAGAGCTTTG GATGCACACA
    GATCCTGTTT CTCAGAAAAA TGAGTCTGTA CGGAACCAGG TGGAGGATCT CCTGGCCACA
    TTGGAGAAGA GTGGTGCTGG TGTCCCTGCT GTCATCCTCA GGAGGCCAAA TCAGTCCCAG
    CCTCTCCCAC CATCTTCCCT GCAGCGATTT CTTCGAGCTC GAAACATCTC TGGCGTTGTT
    CTGGCTGACC ACTCTGGTGC CTTCCATAAC AAATATTACC AGAGTATTTA CGACACTGCT
    GAGAACATTA ATGTGAGCTA TCCCGAATGG CTGAGCCCTG AAGAGGACCT GAACTTTGTA
    ACAGACACTG CCAAGGCCCT GGCAGATGTG GCCACGGTGC TGGGACGTGC TCTGTATGAG
    CTTGCAGGAG GAACCAACTT CAGCGACACA GTTCAGGCTG ATCCCCAAAC GGTTACCCGC
    CTGCTCTATG GGTTCCTGAT TAAAGCCAAC AACTCATGGT TCCAGTCTAT CCTCAGGCAG
    GACCTAAGGT CCTACTTGGG TGACGGGCCT CTTCAACATT ACATCGCTGT CTCCAGCCCC
    ACCAACACCA CTTATGTTGT ACAGTATGCC TTGGCAAATT TGACTGGCAC AGTGGTCAAC
    CTCACCCGAG AGCAGTGCCA GGATCCAAGT AAAGTCCCAA GTGAAAACAA GGATCTGTAT
    GAGTACTCAT GGGTCCAGGG CCCTTTGCAT TCTAATGAGA CGGACCGACT CCCCCGGTGT
    GTGCGTTCTA CTGCACGATT AGCCAGGGCC TTGTCTCCTG CCTTTGAACT GAGTCAGTGG
    AGCTCTACTG AATACTCTAC ATGGACTGAG AGCCGCTGGA AAGATATCCG TGCCCGGATA
    TTTCTCATCG CCAGCAAAGA GCTTGAGTTG ATCACCCTGA CAGTGGGCTT CGGCATCCTC
    ATCTTCTCCC TCATCGTCAC CTACTGCATC AATGCCAAAG CTGATGTCCT TTTCATTGCT
    CCCCGGGAGC CAGGAGCTGT GTCATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001277113.1
    CDS214..2283
    Translation

    Target ORF information:

    RefSeq Version NM_001290184.2
    Organism Homo sapiens(human)
    Definition Homo sapiens nicastrin (NCSTN), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001290184.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACTTTA ATCTCATTTT AGAAAGTTTG TGCAGGGGAA ACTCAGTGGA GAGGAAGATA 
    TATATCCCCT TAAATAAAAC AGCTCCCTGT GTTCGCCTGC TCAACGCCAC TCATCAGATT
    GGCTGCCAGT CTTCAATTAG TGGAGACACA GGGGTTATCC ACGTAGTAGA GAAAGAGGAG
    GACCTACAGT GGGTATTGAC TGATGGCCCC AACCCCCCTT ACATGGTTCT GCTGGAGAGC
    AAGCATTTTA CCAGGGATTT AATGGAGAAG CTGAAAGGGA GAACCAGCCG AATTGCTGGT
    CTTGCAGTGT CCTTGACCAA GCCCAGTCCT GCCTCAGGCT TCTCTCCTAG TGTACAGTGC
    CCAAATGATG GGTTTGGTGT TTACTCCAAT TCCTATGGGC CAGAGTTTGC TCACTGCAGA
    GAAATACAGT GGAATTCGCT GGGCAATGGT TTGGCTTATG AAGACTTTAG TTTCCCCATC
    TTTCTTCTTG AAGATGAAAA TGAAACCAAA GTCATCAAGC AGTGCTATCA AGATCACAAC
    CTGAGTCAGA ATGGCTCAGC ACCAACCTTC CCACTATGTG CCATGCAGCT CTTTTCACAC
    ATGCATGCTG TCATCAGCAC TGCCACCTGC ATGCGGCGCA GCTCCATCCA AAGCACCTTC
    AGCATCAACC CAGAAATCGT CTGTGACCCC CTGTCTGATT ACAATGTGTG GAGCATGCTA
    AAGCCTATAA ATACAACTGG GACATTAAAG CCTGACGACA GGGTTGTGGT TGCTGCCACC
    CGGCTGGATA GTCGTTCCTT TTTCTGGAAT GTGGCCCCAG GGGCTGAAAG CGCAGTGGCT
    TCCTTTGTCA CCCAGCTGGC TGCTGCTGAA GCTTTGCAAA AGGCACCTGA TGTGACCACC
    CTGCCCCGCA ATGTCATGTT TGTCTTCTTT CAAGGGGAAA CTTTTGACTA CATTGGCAGC
    TCGAGGATGG TCTACGATAT GGAGAAGGGC AAGTTTCCCG TGCAGTTAGA GAATGTTGAC
    TCATTTGTGG AGCTGGGACA GGTGGCCTTA AGAACTTCAT TAGAGCTTTG GATGCACACA
    GATCCTGTTT CTCAGAAAAA TGAGTCTGTA CGGAACCAGG TGGAGGATCT CCTGGCCACA
    TTGGAGAAGA GTGGTGCTGG TGTCCCTGCT GTCATCCTCA GGAGGCCAAA TCAGTCCCAG
    CCTCTCCCAC CATCTTCCCT GCAGCGATTT CTTCGAGCTC GAAACATCTC TGGCGTTGTT
    CTGGCTGACC ACTCTGGTGC CTTCCATAAC AAATATTACC AGAGTATTTA CGACACTGCT
    GAGAACATTA ATGTGAGCTA TCCCGAATGG CTGAGCCCTG AAGAGGACCT GAACTTTGTA
    ACAGACACTG CCAAGGCCCT GGCAGATGTG GCCACGGTGC TGGGACGTGC TCTGTATGAG
    CTTGCAGGAG GAACCAACTT CAGCGACACA GTTCAGGCTG ATCCCCAAAC GGTTACCCGC
    CTGCTCTATG GGTTCCTGAT TAAAGCCAAC AACTCATGGT TCCAGTCTAT CCTCAGGCAG
    GACCTAAGGT CCTACTTGGG TGACGGGCCT CTTCAACATT ACATCGCTGT CTCCAGCCCC
    ACCAACACCA CTTATGTTGT ACAGTATGCC TTGGCAAATT TGACTGGCAC AGTGGTCAAC
    CTCACCCGAG AGCAGTGCCA GGATCCAAGT AAAGTCCCAA GTGAAAACAA GGATCTGTAT
    GAGTACTCAT GGGTCCAGGG CCCTTTGCAT TCTAATGAGA CGGACCGACT CCCCCGGTGT
    GTGCGTTCTA CTGCACGATT AGCCAGGGCC TTGTCTCCTG CCTTTGAACT GAGTCAGTGG
    AGCTCTACTG AATACTCTAC ATGGACTGAG AGCCGCTGGA AAGATATCCG TGCCCGGATA
    TTTCTCATCG CCAGCAAAGA GCTTGAGTTG ATCACCCTGA CAGTGGGCTT CGGCATCCTC
    ATCTTCTCCC TCATCGTCAC CTACTGCATC AATGCCAAAG CTGATGTCCT TTTCATTGCT
    CCCCGGGAGC CAGGAGCTGT GTCATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08813
    Clone ID Related Accession (Same CDS sequence) NM_001290184.2 , NM_001290184.1
    Accession Version NM_001290184.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-22
    Organism Homo sapiens(human)
    Product nicastrin isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF240468.1, BC047621.1, AL445230.11 and CN429672.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) has a different 5' structure resulting in translation initiation at an alternate AUG compared to variant 1; the 5'-most AUG, as used in variant 1, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the alternate AUG resulting in a shorter isoform (2) with a distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC047621.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820687 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACTTTA ATCTCATTTT AGAAAGTTTG TGCAGGGGAA ACTCAGTGGA GAGGAAGATA 
    TATATCCCCT TAAATAAAAC AGCTCCCTGT GTTCGCCTGC TCAACGCCAC TCATCAGATT
    GGCTGCCAGT CTTCAATTAG TGGAGACACA GGGGTTATCC ACGTAGTAGA GAAAGAGGAG
    GACCTACAGT GGGTATTGAC TGATGGCCCC AACCCCCCTT ACATGGTTCT GCTGGAGAGC
    AAGCATTTTA CCAGGGATTT AATGGAGAAG CTGAAAGGGA GAACCAGCCG AATTGCTGGT
    CTTGCAGTGT CCTTGACCAA GCCCAGTCCT GCCTCAGGCT TCTCTCCTAG TGTACAGTGC
    CCAAATGATG GGTTTGGTGT TTACTCCAAT TCCTATGGGC CAGAGTTTGC TCACTGCAGA
    GAAATACAGT GGAATTCGCT GGGCAATGGT TTGGCTTATG AAGACTTTAG TTTCCCCATC
    TTTCTTCTTG AAGATGAAAA TGAAACCAAA GTCATCAAGC AGTGCTATCA AGATCACAAC
    CTGAGTCAGA ATGGCTCAGC ACCAACCTTC CCACTATGTG CCATGCAGCT CTTTTCACAC
    ATGCATGCTG TCATCAGCAC TGCCACCTGC ATGCGGCGCA GCTCCATCCA AAGCACCTTC
    AGCATCAACC CAGAAATCGT CTGTGACCCC CTGTCTGATT ACAATGTGTG GAGCATGCTA
    AAGCCTATAA ATACAACTGG GACATTAAAG CCTGACGACA GGGTTGTGGT TGCTGCCACC
    CGGCTGGATA GTCGTTCCTT TTTCTGGAAT GTGGCCCCAG GGGCTGAAAG CGCAGTGGCT
    TCCTTTGTCA CCCAGCTGGC TGCTGCTGAA GCTTTGCAAA AGGCACCTGA TGTGACCACC
    CTGCCCCGCA ATGTCATGTT TGTCTTCTTT CAAGGGGAAA CTTTTGACTA CATTGGCAGC
    TCGAGGATGG TCTACGATAT GGAGAAGGGC AAGTTTCCCG TGCAGTTAGA GAATGTTGAC
    TCATTTGTGG AGCTGGGACA GGTGGCCTTA AGAACTTCAT TAGAGCTTTG GATGCACACA
    GATCCTGTTT CTCAGAAAAA TGAGTCTGTA CGGAACCAGG TGGAGGATCT CCTGGCCACA
    TTGGAGAAGA GTGGTGCTGG TGTCCCTGCT GTCATCCTCA GGAGGCCAAA TCAGTCCCAG
    CCTCTCCCAC CATCTTCCCT GCAGCGATTT CTTCGAGCTC GAAACATCTC TGGCGTTGTT
    CTGGCTGACC ACTCTGGTGC CTTCCATAAC AAATATTACC AGAGTATTTA CGACACTGCT
    GAGAACATTA ATGTGAGCTA TCCCGAATGG CTGAGCCCTG AAGAGGACCT GAACTTTGTA
    ACAGACACTG CCAAGGCCCT GGCAGATGTG GCCACGGTGC TGGGACGTGC TCTGTATGAG
    CTTGCAGGAG GAACCAACTT CAGCGACACA GTTCAGGCTG ATCCCCAAAC GGTTACCCGC
    CTGCTCTATG GGTTCCTGAT TAAAGCCAAC AACTCATGGT TCCAGTCTAT CCTCAGGCAG
    GACCTAAGGT CCTACTTGGG TGACGGGCCT CTTCAACATT ACATCGCTGT CTCCAGCCCC
    ACCAACACCA CTTATGTTGT ACAGTATGCC TTGGCAAATT TGACTGGCAC AGTGGTCAAC
    CTCACCCGAG AGCAGTGCCA GGATCCAAGT AAAGTCCCAA GTGAAAACAA GGATCTGTAT
    GAGTACTCAT GGGTCCAGGG CCCTTTGCAT TCTAATGAGA CGGACCGACT CCCCCGGTGT
    GTGCGTTCTA CTGCACGATT AGCCAGGGCC TTGTCTCCTG CCTTTGAACT GAGTCAGTGG
    AGCTCTACTG AATACTCTAC ATGGACTGAG AGCCGCTGGA AAGATATCCG TGCCCGGATA
    TTTCTCATCG CCAGCAAAGA GCTTGAGTTG ATCACCCTGA CAGTGGGCTT CGGCATCCTC
    ATCTTCTCCC TCATCGTCAC CTACTGCATC AATGCCAAAG CTGATGTCCT TTTCATTGCT
    CCCCGGGAGC CAGGAGCTGT GTCATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001277113.1
    CDS324..2393
    Translation

    Target ORF information:

    RefSeq Version NM_001290184.1
    Organism Homo sapiens(human)
    Definition Homo sapiens nicastrin (NCSTN), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001290184.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACTTTA ATCTCATTTT AGAAAGTTTG TGCAGGGGAA ACTCAGTGGA GAGGAAGATA 
    TATATCCCCT TAAATAAAAC AGCTCCCTGT GTTCGCCTGC TCAACGCCAC TCATCAGATT
    GGCTGCCAGT CTTCAATTAG TGGAGACACA GGGGTTATCC ACGTAGTAGA GAAAGAGGAG
    GACCTACAGT GGGTATTGAC TGATGGCCCC AACCCCCCTT ACATGGTTCT GCTGGAGAGC
    AAGCATTTTA CCAGGGATTT AATGGAGAAG CTGAAAGGGA GAACCAGCCG AATTGCTGGT
    CTTGCAGTGT CCTTGACCAA GCCCAGTCCT GCCTCAGGCT TCTCTCCTAG TGTACAGTGC
    CCAAATGATG GGTTTGGTGT TTACTCCAAT TCCTATGGGC CAGAGTTTGC TCACTGCAGA
    GAAATACAGT GGAATTCGCT GGGCAATGGT TTGGCTTATG AAGACTTTAG TTTCCCCATC
    TTTCTTCTTG AAGATGAAAA TGAAACCAAA GTCATCAAGC AGTGCTATCA AGATCACAAC
    CTGAGTCAGA ATGGCTCAGC ACCAACCTTC CCACTATGTG CCATGCAGCT CTTTTCACAC
    ATGCATGCTG TCATCAGCAC TGCCACCTGC ATGCGGCGCA GCTCCATCCA AAGCACCTTC
    AGCATCAACC CAGAAATCGT CTGTGACCCC CTGTCTGATT ACAATGTGTG GAGCATGCTA
    AAGCCTATAA ATACAACTGG GACATTAAAG CCTGACGACA GGGTTGTGGT TGCTGCCACC
    CGGCTGGATA GTCGTTCCTT TTTCTGGAAT GTGGCCCCAG GGGCTGAAAG CGCAGTGGCT
    TCCTTTGTCA CCCAGCTGGC TGCTGCTGAA GCTTTGCAAA AGGCACCTGA TGTGACCACC
    CTGCCCCGCA ATGTCATGTT TGTCTTCTTT CAAGGGGAAA CTTTTGACTA CATTGGCAGC
    TCGAGGATGG TCTACGATAT GGAGAAGGGC AAGTTTCCCG TGCAGTTAGA GAATGTTGAC
    TCATTTGTGG AGCTGGGACA GGTGGCCTTA AGAACTTCAT TAGAGCTTTG GATGCACACA
    GATCCTGTTT CTCAGAAAAA TGAGTCTGTA CGGAACCAGG TGGAGGATCT CCTGGCCACA
    TTGGAGAAGA GTGGTGCTGG TGTCCCTGCT GTCATCCTCA GGAGGCCAAA TCAGTCCCAG
    CCTCTCCCAC CATCTTCCCT GCAGCGATTT CTTCGAGCTC GAAACATCTC TGGCGTTGTT
    CTGGCTGACC ACTCTGGTGC CTTCCATAAC AAATATTACC AGAGTATTTA CGACACTGCT
    GAGAACATTA ATGTGAGCTA TCCCGAATGG CTGAGCCCTG AAGAGGACCT GAACTTTGTA
    ACAGACACTG CCAAGGCCCT GGCAGATGTG GCCACGGTGC TGGGACGTGC TCTGTATGAG
    CTTGCAGGAG GAACCAACTT CAGCGACACA GTTCAGGCTG ATCCCCAAAC GGTTACCCGC
    CTGCTCTATG GGTTCCTGAT TAAAGCCAAC AACTCATGGT TCCAGTCTAT CCTCAGGCAG
    GACCTAAGGT CCTACTTGGG TGACGGGCCT CTTCAACATT ACATCGCTGT CTCCAGCCCC
    ACCAACACCA CTTATGTTGT ACAGTATGCC TTGGCAAATT TGACTGGCAC AGTGGTCAAC
    CTCACCCGAG AGCAGTGCCA GGATCCAAGT AAAGTCCCAA GTGAAAACAA GGATCTGTAT
    GAGTACTCAT GGGTCCAGGG CCCTTTGCAT TCTAATGAGA CGGACCGACT CCCCCGGTGT
    GTGCGTTCTA CTGCACGATT AGCCAGGGCC TTGTCTCCTG CCTTTGAACT GAGTCAGTGG
    AGCTCTACTG AATACTCTAC ATGGACTGAG AGCCGCTGGA AAGATATCCG TGCCCGGATA
    TTTCTCATCG CCAGCAAAGA GCTTGAGTTG ATCACCCTGA CAGTGGGCTT CGGCATCCTC
    ATCTTCTCCC TCATCGTCAC CTACTGCATC AATGCCAAAG CTGATGTCCT TTTCATTGCT
    CCCCGGGAGC CAGGAGCTGT GTCATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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