CRB1 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following CRB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu66169 XM_011509369.2
Latest version!
Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $475.30
$679.00
OHu22808 NM_001257966.1
Latest version!
Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu19399 NM_201253.2
Latest version!
Homo sapiens crumbs 1, cell polarity complex component (CRB1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $727.30
$1039.00
OHu107688 NM_201253.3
Latest version!
Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu108189 NM_001193640.2
Latest version!
Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu108193 NM_001257965.2
Latest version!
Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu87140 XM_017000851.1
Latest version!
Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu87141 XM_017000852.1
Latest version!
Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu17475 NM_001193640.1
Latest version!
Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu66167 XM_011509367.1
Latest version!
Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu22677 NM_001257965.1
Latest version!
Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu66165 XM_011509365.2
Latest version!
Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu66169
    Clone ID Related Accession (Same CDS sequence) XM_011509369.2
    Accession Version XM_011509369.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2664bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein crumbs homolog 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011509369.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCTCTTC TACTTTTCCG AAGCAACAGG GATGTGTTTG TGAAGCTGGA GCTGCTAAGT 
    GGCTACATTC ACTTATCAAT TCAGGTCAAT AATCAGTCAA AGGTGCTTCT GTTCATTTCC
    CACAACACCA GCGATGGAGA GTGGCATTTC GTGGAGGTAA TATTTGCAGA GGCTGTGACC
    CTTACCTTAA TCGACGACTC CTGTAAGGAG AAATGCATCG CGAAAGCTCC TACTCCACTT
    GAAAGTGATC AATCAATATG TGCTTTTCAG AACTCCTTTT TGGGTGGTTT ACCAGTGGGA
    ATGACCAGCA ATGGTGTTGC TCTGCTTAAC TTCTATAATA TGCCATCCAC ACCTTCGTTT
    GTAGGCTGTC TCCAAGACAT TAAAATTGAT TGGAATCACA TTACCCTGGA GAACATCTCG
    TCTGGCTCAT CATTAAATGT CAAGGCAGGC TGTGTGAGAA AGGATTGGTG TGAAAGCCAA
    CCTTGTCAAA GCAGAGGACG CTGCATCAAC TTGTGGCTGA GTTACCAGTG TGACTGCCAC
    AGGCCCTATG AAGGCCCCAA CTGTCTGAGA GAGTATGTGG CAGGCAGATT TGGCCAGGAT
    GACTCCACTG GTTATGTCAT CTTTACTCTT GATGAGAGCT ATGGAGACAC CATCAGCCTC
    TCCATGTTTG TCCGAACGCT TCAACCATCA GGCTTACTTC TAGCTTTGGA AAACAGCACT
    TATCAATATA TCCGTGTCTG GCTAGAGCGC GGCAGACTAG CAATGCTGAC TCCAAACTCT
    CCCAAATTAG TAGTAAAATT TGTTCTTAAT GATGGAAATG TCCACTTGAT ATCTTTGAAA
    ATCAAGCCAT ATAAAATTGA ACTGTATCAG TCTTCACAAA ACCTAGGATT TATTTCTGCT
    TCTACGTGGA AAATCGAAAA GGGAGATGTC ATCTACATTG GTGGCCTACC TGACAAGCAA
    GAGACTGAAC TTAATGGTGG ATTCTTCAAA GGCTGTATCC AAGATGTAAG ACTAAACAAC
    CAAAATCTGG AATTCTTTCC AAATCCAACA AACAATGCAT CTCTCAATCC AGTTCTTGTC
    AATGTAACCC AAGGCTGTGC TGGAGACAAC AGCTGCAAGT CCAACCCCTG TCACAATGGA
    GGTGTTTGCC ATTCCCGGTG GGATGACTTC TCCTGTTCCT GTCCTGCCCT CACAAGTGGG
    AAAGCCTGTG AGGAGGTTCA GTGGTGTGGA TTCAGCCCGT GTCCTCACGG AGCCCAGTGC
    CAGCCGGTGC TTCAAGGATT TGAATGTATT GCAAATGCTG TTTTTAATGG ACAAAGCGGT
    CAAATATTAT TCAGAAGCAA TGGGAATATT ACCAGAGAAC TCACCAATAT CACATTTGGT
    TTCAGAACAA GGGATGCAAA TGTAATAATA TTGCATGCAG AAAAAGAGCC TGAATTTCTT
    AATATTAGCA TTCAAGATTC CAGATTATTC TTTCAATTGC AAAGTGGCAA CAGCTTTTAT
    ATGCTAAGTC TGACAAGTTT GCAGTCAGTG AATGATGGCA CATGGCACGA AGTGACCCTT
    TCCATGACAG ACCCACTGTC CCAGACCTCC AGGTGGCAAA TGGAAGTGGA CAACGAAACA
    CCTTTTGTGA CCAGCACAAT TGCTACTGGA AGCCTCAACT TTTTGAAGGA TAATACAGAT
    ATTTATGTGG GAGACAGAGC TATTGACAAT ATAAAGGGCC TGCAAGGGTG TCTAAGTACA
    ATAGAAATCG GAGGCATTTA TCTCTCTTAC TTTGAAAATG TTCATGGTTT CATTAATAAA
    CCTCAGGAAG AGCAATTTCT CAAAATCTCT ACCAATTCAG TGGTCACTGG CTGTTTGCAG
    TTAAATGTCT GCAACTCCAA CCCCTGTTTG CATGGAGGAA ACTGTGAAGA CATCTATAGC
    TCTTATCATT GCTCCTGTCC CTTGGGATGG TCAGGGAAAC ACTGTGAACT CAACATCGAT
    GAATGCTTTT CAAACCCCTG TATCCATGGC AACTGCTCTG ACAGAGTTGC AGCCTACCAC
    TGCACATGTG AGCCTGGATA CACTGGTGTG AACTGTGAAG TGGATATAGA CAACTGCCAG
    AGTCACCAGT GTGCAAATGG AGCCACCTGC ATTAGTCATA CTAATGGCTA TTCTTGCCTC
    TGTTTTGGAA ATTTTACAGG AAAATTTTGC AGACAGAGCA GATTACCCTC AACAGTCTGT
    GGGAATGAGA AGACAAATCT CACTTGCTAC AATGGAGGCA ACTGCACAGA GTTCCAGACT
    GAATTAAAAT GTATGTGCCG GCCAGGTTTT ACTGGAGAAT GGTGTGAAAA GGACATTGAT
    GAGTGTGCCT CTGATCCGTG TGTCAATGGA GGTCTGTGCC AGGACTTACT CAACAAATTC
    CAGTGCCTCT GTGATGTTGC CTTTGCTGGC GAGCGCTGCG AGGTGGACTT GGCAGATGAC
    TTGATCTCCG ACATTTTCAC CACTATTGGC TCAGTGACTG TCGCCTTGTT ACTGATCCTC
    TTGCTGGCCA TTGTTGCTTC TGTTGTCACC TCCAACAAAA GGGCAACTCA GGGAACCTAC
    AGCCCCAGCC GTCAGGAGAA GGAGGGCTCC CGAGTGGAAA TGTGGAACTT GATGCCACCC
    CCTGCAATGG AGAGACTGAT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011507671.1
    CDS480..3143
    Translation

    Target ORF information:

    RefSeq Version XM_011509369.2
    Organism Homo sapiens(human)
    Definition Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011509369.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCTCTTC TACTTTTCCG AAGCAACAGG GATGTGTTTG TGAAGCTGGA GCTGCTAAGT 
    GGCTACATTC ACTTATCAAT TCAGGTCAAT AATCAGTCAA AGGTGCTTCT GTTCATTTCC
    CACAACACCA GCGATGGAGA GTGGCATTTC GTGGAGGTAA TATTTGCAGA GGCTGTGACC
    CTTACCTTAA TCGACGACTC CTGTAAGGAG AAATGCATCG CGAAAGCTCC TACTCCACTT
    GAAAGTGATC AATCAATATG TGCTTTTCAG AACTCCTTTT TGGGTGGTTT ACCAGTGGGA
    ATGACCAGCA ATGGTGTTGC TCTGCTTAAC TTCTATAATA TGCCATCCAC ACCTTCGTTT
    GTAGGCTGTC TCCAAGACAT TAAAATTGAT TGGAATCACA TTACCCTGGA GAACATCTCG
    TCTGGCTCAT CATTAAATGT CAAGGCAGGC TGTGTGAGAA AGGATTGGTG TGAAAGCCAA
    CCTTGTCAAA GCAGAGGACG CTGCATCAAC TTGTGGCTGA GTTACCAGTG TGACTGCCAC
    AGGCCCTATG AAGGCCCCAA CTGTCTGAGA GAGTATGTGG CAGGCAGATT TGGCCAGGAT
    GACTCCACTG GTTATGTCAT CTTTACTCTT GATGAGAGCT ATGGAGACAC CATCAGCCTC
    TCCATGTTTG TCCGAACGCT TCAACCATCA GGCTTACTTC TAGCTTTGGA AAACAGCACT
    TATCAATATA TCCGTGTCTG GCTAGAGCGC GGCAGACTAG CAATGCTGAC TCCAAACTCT
    CCCAAATTAG TAGTAAAATT TGTTCTTAAT GATGGAAATG TCCACTTGAT ATCTTTGAAA
    ATCAAGCCAT ATAAAATTGA ACTGTATCAG TCTTCACAAA ACCTAGGATT TATTTCTGCT
    TCTACGTGGA AAATCGAAAA GGGAGATGTC ATCTACATTG GTGGCCTACC TGACAAGCAA
    GAGACTGAAC TTAATGGTGG ATTCTTCAAA GGCTGTATCC AAGATGTAAG ACTAAACAAC
    CAAAATCTGG AATTCTTTCC AAATCCAACA AACAATGCAT CTCTCAATCC AGTTCTTGTC
    AATGTAACCC AAGGCTGTGC TGGAGACAAC AGCTGCAAGT CCAACCCCTG TCACAATGGA
    GGTGTTTGCC ATTCCCGGTG GGATGACTTC TCCTGTTCCT GTCCTGCCCT CACAAGTGGG
    AAAGCCTGTG AGGAGGTTCA GTGGTGTGGA TTCAGCCCGT GTCCTCACGG AGCCCAGTGC
    CAGCCGGTGC TTCAAGGATT TGAATGTATT GCAAATGCTG TTTTTAATGG ACAAAGCGGT
    CAAATATTAT TCAGAAGCAA TGGGAATATT ACCAGAGAAC TCACCAATAT CACATTTGGT
    TTCAGAACAA GGGATGCAAA TGTAATAATA TTGCATGCAG AAAAAGAGCC TGAATTTCTT
    AATATTAGCA TTCAAGATTC CAGATTATTC TTTCAATTGC AAAGTGGCAA CAGCTTTTAT
    ATGCTAAGTC TGACAAGTTT GCAGTCAGTG AATGATGGCA CATGGCACGA AGTGACCCTT
    TCCATGACAG ACCCACTGTC CCAGACCTCC AGGTGGCAAA TGGAAGTGGA CAACGAAACA
    CCTTTTGTGA CCAGCACAAT TGCTACTGGA AGCCTCAACT TTTTGAAGGA TAATACAGAT
    ATTTATGTGG GAGACAGAGC TATTGACAAT ATAAAGGGCC TGCAAGGGTG TCTAAGTACA
    ATAGAAATCG GAGGCATTTA TCTCTCTTAC TTTGAAAATG TTCATGGTTT CATTAATAAA
    CCTCAGGAAG AGCAATTTCT CAAAATCTCT ACCAATTCAG TGGTCACTGG CTGTTTGCAG
    TTAAATGTCT GCAACTCCAA CCCCTGTTTG CATGGAGGAA ACTGTGAAGA CATCTATAGC
    TCTTATCATT GCTCCTGTCC CTTGGGATGG TCAGGGAAAC ACTGTGAACT CAACATCGAT
    GAATGCTTTT CAAACCCCTG TATCCATGGC AACTGCTCTG ACAGAGTTGC AGCCTACCAC
    TGCACATGTG AGCCTGGATA CACTGGTGTG AACTGTGAAG TGGATATAGA CAACTGCCAG
    AGTCACCAGT GTGCAAATGG AGCCACCTGC ATTAGTCATA CTAATGGCTA TTCTTGCCTC
    TGTTTTGGAA ATTTTACAGG AAAATTTTGC AGACAGAGCA GATTACCCTC AACAGTCTGT
    GGGAATGAGA AGACAAATCT CACTTGCTAC AATGGAGGCA ACTGCACAGA GTTCCAGACT
    GAATTAAAAT GTATGTGCCG GCCAGGTTTT ACTGGAGAAT GGTGTGAAAA GGACATTGAT
    GAGTGTGCCT CTGATCCGTG TGTCAATGGA GGTCTGTGCC AGGACTTACT CAACAAATTC
    CAGTGCCTCT GTGATGTTGC CTTTGCTGGC GAGCGCTGCG AGGTGGACTT GGCAGATGAC
    TTGATCTCCG ACATTTTCAC CACTATTGGC TCAGTGACTG TCGCCTTGTT ACTGATCCTC
    TTGCTGGCCA TTGTTGCTTC TGTTGTCACC TCCAACAAAA GGGCAACTCA GGGAACCTAC
    AGCCCCAGCC GTCAGGAGAA GGAGGGCTCC CGAGTGGAAA TGTGGAACTT GATGCCACCC
    CCTGCAATGG AGAGACTGAT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22808
    Clone ID Related Accession (Same CDS sequence) NM_001257966.1
    Accession Version NM_001257966.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2613bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product protein crumbs homolog 1 isoform 4 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC370545.1, AL513325.13, AK299368.1 and AY043325.1. Transcript Variant: This variant (4) lacks an alternate in-frame segment of two coding exons and most of a third compared to variant 1. The resulting isoform (4) has the same N- and C-termini but lacks an alternate internal segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK299368.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC
    TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG
    GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA
    CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA
    GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC
    TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT
    GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC
    AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG
    AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT
    TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA
    TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT
    TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT
    ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC
    CAAGATGGCC AGCATGGATT CAGCTGCCTG TGTCCATCTG GCTACACCGG GTCCCTGTGT
    GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA
    GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG
    GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC
    TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC
    AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT
    ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA
    AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG
    ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA
    GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT
    GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT
    TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG
    CCCTATGAAG GCCCCAACTG TCTGAGAGGA AAATTTTGCA GACAGAGCAG ATTACCCTCA
    ACAGTCTGTG GGAATGAGAA GACAAATCTC ACTTGCTACA ATGGAGGCAA CTGCACAGAG
    TTCCAGACTG AATTAAAATG TATGTGCCGG CCAGGTTTTA CTGGAGAATG GTGTGAAAAG
    GACATTGATG AGTGTGCCTC TGATCCGTGT GTCAATGGAG GTCTGTGCCA GGACTTACTC
    AACAAATTCC AGTGCCTCTG TGATGTTGCC TTTGCTGGCG AGCGCTGCGA GGTGGACTTG
    GCAGATGACT TGATCTCCGA CATTTTCACC ACTATTGGCT CAGTGACTGT CGCCTTGTTA
    CTGATCCTCT TGCTGGCCAT TGTTGCTTCT GTTGTCACCT CCAACAAAAG GGCAACTCAG
    GGAACCTACA GCCCCAGCCG TCAGGAGAAG GAGGGCTCCC GAGTGGAAAT GTGGAACTTG
    ATGCCACCCC CTGCAATGGA GAGACTGATT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001244895.1
    CDS210..2822
    Translation

    Target ORF information:

    RefSeq Version NM_001257966.1
    Organism Homo sapiens(human)
    Definition Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001257966.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC
    TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG
    GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA
    CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA
    GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC
    TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT
    GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC
    AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG
    AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT
    TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA
    TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT
    TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT
    ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC
    CAAGATGGCC AGCATGGATT CAGCTGCCTG TGTCCATCTG GCTACACCGG GTCCCTGTGT
    GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA
    GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG
    GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC
    TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC
    AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT
    ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA
    AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG
    ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA
    GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT
    GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT
    TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG
    CCCTATGAAG GCCCCAACTG TCTGAGAGGA AAATTTTGCA GACAGAGCAG ATTACCCTCA
    ACAGTCTGTG GGAATGAGAA GACAAATCTC ACTTGCTACA ATGGAGGCAA CTGCACAGAG
    TTCCAGACTG AATTAAAATG TATGTGCCGG CCAGGTTTTA CTGGAGAATG GTGTGAAAAG
    GACATTGATG AGTGTGCCTC TGATCCGTGT GTCAATGGAG GTCTGTGCCA GGACTTACTC
    AACAAATTCC AGTGCCTCTG TGATGTTGCC TTTGCTGGCG AGCGCTGCGA GGTGGACTTG
    GCAGATGACT TGATCTCCGA CATTTTCACC ACTATTGGCT CAGTGACTGT CGCCTTGTTA
    CTGATCCTCT TGCTGGCCAT TGTTGCTTCT GTTGTCACCT CCAACAAAAG GGCAACTCAG
    GGAACCTACA GCCCCAGCCG TCAGGAGAAG GAGGGCTCCC GAGTGGAAAT GTGGAACTTG
    ATGCCACCCC CTGCAATGGA GAGACTGATT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19399
    Clone ID Related Accession (Same CDS sequence) NM_201253.2
    Accession Version NM_201253.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4221bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-05
    Organism Homo sapiens(human)
    Product protein crumbs homolog 1 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC370545.1, DC370777.1, BC136271.1 and AY043325.1. This sequence is a reference standard in the RefSeqGene project. On Aug 7, 2010 this sequence version replaced NM_201253.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY043325.1, SRR1803617.203976.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC
    TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG
    GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA
    CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA
    GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC
    TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT
    GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC
    AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG
    AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT
    TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA
    TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT
    TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT
    ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC
    CAAGATGGCC AGCATGGATT CAGCTGCCTG TGTCCATCTG GCTACACCGG GTCCCTGTGT
    GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA
    GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG
    GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC
    TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC
    AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT
    ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA
    AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG
    ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA
    GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT
    GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT
    TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG
    CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC
    TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC
    ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT
    CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC
    AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC
    AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT
    ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG
    ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA
    AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT
    GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGTCCA ACCCCTGTCA CAATGGAGGT
    GTTTGCCATT CCCGGTGGGA TGACTTCTCC TGTTCCTGTC CTGCCCTCAC AAGTGGGAAA
    GCCTGTGAGG AGGTTCAGTG GTGTGGATTC AGCCCGTGTC CTCACGGAGC CCAGTGCCAG
    CCGGTGCTTC AAGGATTTGA ATGTATTGCA AATGCTGTTT TTAATGGACA AAGCGGTCAA
    ATATTATTCA GAAGCAATGG GAATATTACC AGAGAACTCA CCAATATCAC ATTTGGTTTC
    AGAACAAGGG ATGCAAATGT AATAATATTG CATGCAGAAA AAGAGCCTGA ATTTCTTAAT
    ATTAGCATTC AAGATTCCAG ATTATTCTTT CAATTGCAAA GTGGCAACAG CTTTTATATG
    CTAAGTCTGA CAAGTTTGCA GTCAGTGAAT GATGGCACAT GGCACGAAGT GACCCTTTCC
    ATGACAGACC CACTGTCCCA GACCTCCAGG TGGCAAATGG AAGTGGACAA CGAAACACCT
    TTTGTGACCA GCACAATTGC TACTGGAAGC CTCAACTTTT TGAAGGATAA TACAGATATT
    TATGTGGGAG ACAGAGCTAT TGACAATATA AAGGGCCTGC AAGGGTGTCT AAGTACAATA
    GAAATCGGAG GCATTTATCT CTCTTACTTT GAAAATGTTC ATGGTTTCAT TAATAAACCT
    CAGGAAGAGC AATTTCTCAA AATCTCTACC AATTCAGTGG TCACTGGCTG TTTGCAGTTA
    AATGTCTGCA ACTCCAACCC CTGTTTGCAT GGAGGAAACT GTGAAGACAT CTATAGCTCT
    TATCATTGCT CCTGTCCCTT GGGATGGTCA GGGAAACACT GTGAACTCAA CATCGATGAA
    TGCTTTTCAA ACCCCTGTAT CCATGGCAAC TGCTCTGACA GAGTTGCAGC CTACCACTGC
    ACATGTGAGC CTGGATACAC TGGTGTGAAC TGTGAAGTGG ATATAGACAA CTGCCAGAGT
    CACCAGTGTG CAAATGGAGC CACCTGCATT AGTCATACTA ATGGCTATTC TTGCCTCTGT
    TTTGGAAATT TTACAGGAAA ATTTTGCAGA CAGAGCAGAT TACCCTCAAC AGTCTGTGGG
    AATGAGAAGA CAAATCTCAC TTGCTACAAT GGAGGCAACT GCACAGAGTT CCAGACTGAA
    TTAAAATGTA TGTGCCGGCC AGGTTTTACT GGAGAATGGT GTGAAAAGGA CATTGATGAG
    TGTGCCTCTG ATCCGTGTGT CAATGGAGGT CTGTGCCAGG ACTTACTCAA CAAATTCCAG
    TGCCTCTGTG ATGTTGCCTT TGCTGGCGAG CGCTGCGAGG TGGACTTGGC AGATGACTTG
    ATCTCCGACA TTTTCACCAC TATTGGCTCA GTGACTGTCG CCTTGTTACT GATCCTCTTG
    CTGGCCATTG TTGCTTCTGT TGTCACCTCC AACAAAAGGG CAACTCAGGG AACCTACAGC
    CCCAGCCGTC AGGAGAAGGA GGGCTCCCGA GTGGAAATGT GGAACTTGAT GCCACCCCCT
    GCAATGGAGA GACTGATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_957705.1
    CDS210..4430
    Translation

    Target ORF information:

    RefSeq Version NM_201253.2
    Organism Homo sapiens(human)
    Definition Homo sapiens crumbs 1, cell polarity complex component (CRB1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_201253.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC
    TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG
    GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA
    CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA
    GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC
    TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT
    GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC
    AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG
    AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT
    TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA
    TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT
    TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT
    ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC
    CAAGATGGCC AGCATGGATT CAGCTGCCTG TGTCCATCTG GCTACACCGG GTCCCTGTGT
    GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA
    GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG
    GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC
    TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC
    AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT
    ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA
    AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG
    ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA
    GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT
    GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT
    TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG
    CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC
    TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC
    ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT
    CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC
    AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC
    AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT
    ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG
    ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA
    AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT
    GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGTCCA ACCCCTGTCA CAATGGAGGT
    GTTTGCCATT CCCGGTGGGA TGACTTCTCC TGTTCCTGTC CTGCCCTCAC AAGTGGGAAA
    GCCTGTGAGG AGGTTCAGTG GTGTGGATTC AGCCCGTGTC CTCACGGAGC CCAGTGCCAG
    CCGGTGCTTC AAGGATTTGA ATGTATTGCA AATGCTGTTT TTAATGGACA AAGCGGTCAA
    ATATTATTCA GAAGCAATGG GAATATTACC AGAGAACTCA CCAATATCAC ATTTGGTTTC
    AGAACAAGGG ATGCAAATGT AATAATATTG CATGCAGAAA AAGAGCCTGA ATTTCTTAAT
    ATTAGCATTC AAGATTCCAG ATTATTCTTT CAATTGCAAA GTGGCAACAG CTTTTATATG
    CTAAGTCTGA CAAGTTTGCA GTCAGTGAAT GATGGCACAT GGCACGAAGT GACCCTTTCC
    ATGACAGACC CACTGTCCCA GACCTCCAGG TGGCAAATGG AAGTGGACAA CGAAACACCT
    TTTGTGACCA GCACAATTGC TACTGGAAGC CTCAACTTTT TGAAGGATAA TACAGATATT
    TATGTGGGAG ACAGAGCTAT TGACAATATA AAGGGCCTGC AAGGGTGTCT AAGTACAATA
    GAAATCGGAG GCATTTATCT CTCTTACTTT GAAAATGTTC ATGGTTTCAT TAATAAACCT
    CAGGAAGAGC AATTTCTCAA AATCTCTACC AATTCAGTGG TCACTGGCTG TTTGCAGTTA
    AATGTCTGCA ACTCCAACCC CTGTTTGCAT GGAGGAAACT GTGAAGACAT CTATAGCTCT
    TATCATTGCT CCTGTCCCTT GGGATGGTCA GGGAAACACT GTGAACTCAA CATCGATGAA
    TGCTTTTCAA ACCCCTGTAT CCATGGCAAC TGCTCTGACA GAGTTGCAGC CTACCACTGC
    ACATGTGAGC CTGGATACAC TGGTGTGAAC TGTGAAGTGG ATATAGACAA CTGCCAGAGT
    CACCAGTGTG CAAATGGAGC CACCTGCATT AGTCATACTA ATGGCTATTC TTGCCTCTGT
    TTTGGAAATT TTACAGGAAA ATTTTGCAGA CAGAGCAGAT TACCCTCAAC AGTCTGTGGG
    AATGAGAAGA CAAATCTCAC TTGCTACAAT GGAGGCAACT GCACAGAGTT CCAGACTGAA
    TTAAAATGTA TGTGCCGGCC AGGTTTTACT GGAGAATGGT GTGAAAAGGA CATTGATGAG
    TGTGCCTCTG ATCCGTGTGT CAATGGAGGT CTGTGCCAGG ACTTACTCAA CAAATTCCAG
    TGCCTCTGTG ATGTTGCCTT TGCTGGCGAG CGCTGCGAGG TGGACTTGGC AGATGACTTG
    ATCTCCGACA TTTTCACCAC TATTGGCTCA GTGACTGTCG CCTTGTTACT GATCCTCTTG
    CTGGCCATTG TTGCTTCTGT TGTCACCTCC AACAAAAGGG CAACTCAGGG AACCTACAGC
    CCCAGCCGTC AGGAGAAGGA GGGCTCCCGA GTGGAAATGT GGAACTTGAT GCCACCCCCT
    GCAATGGAGA GACTGATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu107688
    Clone ID Related Accession (Same CDS sequence) NM_201253.3
    Accession Version NM_201253.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4221bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-01
    Organism Homo sapiens(human)
    Product protein crumbs homolog 1 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL513325.13, AL136322.24 and AL139136.17. On Nov 23, 2018 this sequence version replaced NM_201253.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY043325.1, SRR1803613.203862.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000367400.8/ ENSP00000356370.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC
    TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG
    GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA
    CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA
    GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC
    TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT
    GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC
    AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG
    AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT
    TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA
    TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT
    TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT
    ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC
    CAAGATGGCC AGCATGGATT CAGCTGCCTA TGTCCATCTG GCTACACCGG GTCCCTGTGT
    GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA
    GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG
    GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC
    TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC
    AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT
    ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA
    AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG
    ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA
    GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT
    GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT
    TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG
    CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC
    TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC
    ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT
    CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC
    AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC
    AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT
    ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG
    ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA
    AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT
    GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGTCCA ACCCCTGTCA CAATGGAGGT
    GTTTGCCATT CCCGGTGGGA TGACTTCTCC TGTTCCTGTC CTGCCCTCAC AAGTGGGAAA
    GCCTGTGAGG AGGTTCAGTG GTGTGGATTC AGCCCGTGTC CTCACGGAGC CCAGTGCCAG
    CCGGTGCTTC AAGGATTTGA ATGTATTGCA AATGCTGTTT TTAATGGACA AAGCGGTCAA
    ATATTATTCA GAAGCAATGG GAATATTACC AGAGAACTCA CCAATATCAC ATTTGGTTTC
    AGAACAAGGG ATGCAAATGT AATAATATTG CATGCAGAAA AAGAGCCTGA ATTTCTTAAT
    ATTAGCATTC AAGATTCCAG ATTATTCTTT CAATTGCAAA GTGGCAACAG CTTTTATATG
    CTAAGTCTGA CAAGTTTGCA GTCAGTGAAT GATGGCACAT GGCACGAAGT GACCCTTTCC
    ATGACAGACC CACTGTCCCA GACCTCCAGG TGGCAAATGG AAGTGGACAA CGAAACACCT
    TTTGTGACCA GCACAATTGC TACTGGAAGC CTCAACTTTT TGAAGGATAA TACAGATATT
    TATGTGGGAG ACAGAGCTAT TGACAATATA AAGGGCCTGC AAGGGTGTCT AAGTACAATA
    GAAATCGGAG GCATTTATCT CTCTTACTTT GAAAATGTTC ATGGTTTCAT TAATAAACCT
    CAGGAAGAGC AATTTCTCAA AATCTCTACC AATTCAGTGG TCACTGGCTG TTTGCAGTTA
    AATGTCTGCA ACTCCAACCC CTGTTTGCAT GGAGGAAACT GTGAAGACAT CTATAGCTCT
    TATCATTGCT CCTGTCCCTT GGGATGGTCA GGGAAACACT GTGAACTCAA CATCGATGAA
    TGCTTTTCAA ACCCCTGTAT CCATGGCAAC TGCTCTGACA GAGTTGCAGC CTACCACTGC
    ACATGTGAGC CTGGATACAC TGGTGTGAAC TGTGAAGTGG ATATAGACAA CTGCCAGAGT
    CACCAGTGTG CAAATGGAGC CACCTGCATT AGTCATACTA ATGGCTATTC TTGCCTCTGT
    TTTGGAAATT TTACAGGAAA ATTTTGCAGA CAGAGCAGAT TACCCTCAAC AGTCTGTGGG
    AATGAGAAGA CAAATCTCAC TTGCTACAAT GGAGGCAACT GCACAGAGTT CCAGACTGAA
    TTAAAATGTA TGTGCCGGCC AGGTTTTACT GGAGAATGGT GTGAAAAGGA CATTGATGAG
    TGTGCCTCTG ATCCGTGTGT CAATGGAGGT CTGTGCCAGG ACTTACTCAA CAAATTCCAG
    TGCCTCTGTG ATGTTGCCTT TGCTGGCGAG CGCTGCGAGG TGGACTTGGC AGATGACTTG
    ATCTCCGACA TTTTCACCAC TATTGGCTCA GTGACTGTCG CCTTGTTACT GATCCTCTTG
    CTGGCCATTG TTGCTTCTGT TGTCACCTCC AACAAAAGGG CAACTCAGGG AACCTACAGC
    CCCAGCCGTC AGGAGAAGGA GGGCTCCCGA GTGGAAATGT GGAACTTGAT GCCACCCCCT
    GCAATGGAGA GACTGATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_957705.1
    CDS162..4382
    Translation

    Target ORF information:

    RefSeq Version NM_201253.3
    Organism Homo sapiens(human)
    Definition Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_201253.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC
    TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG
    GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA
    CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA
    GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC
    TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT
    GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC
    AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG
    AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT
    TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA
    TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT
    TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT
    ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC
    CAAGATGGCC AGCATGGATT CAGCTGCCTA TGTCCATCTG GCTACACCGG GTCCCTGTGT
    GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA
    GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG
    GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC
    TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC
    AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT
    ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA
    AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG
    ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA
    GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT
    GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT
    TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG
    CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC
    TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC
    ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT
    CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC
    AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC
    AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT
    ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG
    ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA
    AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT
    GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGTCCA ACCCCTGTCA CAATGGAGGT
    GTTTGCCATT CCCGGTGGGA TGACTTCTCC TGTTCCTGTC CTGCCCTCAC AAGTGGGAAA
    GCCTGTGAGG AGGTTCAGTG GTGTGGATTC AGCCCGTGTC CTCACGGAGC CCAGTGCCAG
    CCGGTGCTTC AAGGATTTGA ATGTATTGCA AATGCTGTTT TTAATGGACA AAGCGGTCAA
    ATATTATTCA GAAGCAATGG GAATATTACC AGAGAACTCA CCAATATCAC ATTTGGTTTC
    AGAACAAGGG ATGCAAATGT AATAATATTG CATGCAGAAA AAGAGCCTGA ATTTCTTAAT
    ATTAGCATTC AAGATTCCAG ATTATTCTTT CAATTGCAAA GTGGCAACAG CTTTTATATG
    CTAAGTCTGA CAAGTTTGCA GTCAGTGAAT GATGGCACAT GGCACGAAGT GACCCTTTCC
    ATGACAGACC CACTGTCCCA GACCTCCAGG TGGCAAATGG AAGTGGACAA CGAAACACCT
    TTTGTGACCA GCACAATTGC TACTGGAAGC CTCAACTTTT TGAAGGATAA TACAGATATT
    TATGTGGGAG ACAGAGCTAT TGACAATATA AAGGGCCTGC AAGGGTGTCT AAGTACAATA
    GAAATCGGAG GCATTTATCT CTCTTACTTT GAAAATGTTC ATGGTTTCAT TAATAAACCT
    CAGGAAGAGC AATTTCTCAA AATCTCTACC AATTCAGTGG TCACTGGCTG TTTGCAGTTA
    AATGTCTGCA ACTCCAACCC CTGTTTGCAT GGAGGAAACT GTGAAGACAT CTATAGCTCT
    TATCATTGCT CCTGTCCCTT GGGATGGTCA GGGAAACACT GTGAACTCAA CATCGATGAA
    TGCTTTTCAA ACCCCTGTAT CCATGGCAAC TGCTCTGACA GAGTTGCAGC CTACCACTGC
    ACATGTGAGC CTGGATACAC TGGTGTGAAC TGTGAAGTGG ATATAGACAA CTGCCAGAGT
    CACCAGTGTG CAAATGGAGC CACCTGCATT AGTCATACTA ATGGCTATTC TTGCCTCTGT
    TTTGGAAATT TTACAGGAAA ATTTTGCAGA CAGAGCAGAT TACCCTCAAC AGTCTGTGGG
    AATGAGAAGA CAAATCTCAC TTGCTACAAT GGAGGCAACT GCACAGAGTT CCAGACTGAA
    TTAAAATGTA TGTGCCGGCC AGGTTTTACT GGAGAATGGT GTGAAAAGGA CATTGATGAG
    TGTGCCTCTG ATCCGTGTGT CAATGGAGGT CTGTGCCAGG ACTTACTCAA CAAATTCCAG
    TGCCTCTGTG ATGTTGCCTT TGCTGGCGAG CGCTGCGAGG TGGACTTGGC AGATGACTTG
    ATCTCCGACA TTTTCACCAC TATTGGCTCA GTGACTGTCG CCTTGTTACT GATCCTCTTG
    CTGGCCATTG TTGCTTCTGT TGTCACCTCC AACAAAAGGG CAACTCAGGG AACCTACAGC
    CCCAGCCGTC AGGAGAAGGA GGGCTCCCGA GTGGAAATGT GGAACTTGAT GCCACCCCCT
    GCAATGGAGA GACTGATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108189
    Clone ID Related Accession (Same CDS sequence) NM_001193640.2
    Accession Version NM_001193640.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product protein crumbs homolog 1 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL513325.13, AL136322.24 and AL139136.17. On May 31, 2019 this sequence version replaced NM_001193640.1. Transcript Variant: This variant (2) lacks two in-frame exons compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.211656.1, SRR1803617.209649.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157437, SAMN03465402 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGATACACA
    GGTGCCCAGT GTGAGATCGA CCTCAATGAA TGCAATAGTA ACCCCTGCCA GTCCAATGGG
    GAATGTGTGG AGCTGTCCTC AGAGAAACAA TATGGACGCA TCACTGGACT GCCTTCTTCT
    TTCAGCTACC ATGAAGCCTC AGGTTATGTC TGTATCTGTC AGCCTGGATT CACAGGAATC
    CACTGCGAAG AAGACGTCAA TGAATGTTCT TCAAACCCTT GCCAAAATGG TGGTACTTGT
    GAGAACTTGC CTGGGAATTA TACTTGCCAT TGCCCATTTG ATAACCTTTC TAGAACTTTT
    TATGGAGGAA GGGACTGTTC TGATATTCTC CTGGGCTGTA CCCATCAGCA ATGTCTAAAT
    AATGGAACAT GCATCCCTCA CTTCCAAGAT GGCCAGCATG GATTCAGCTG CCTATGTCCA
    TCTGGCTACA CCGGGTCCCT GTGTGAAATC GCAACCACAC TTTCATTTGA GGGCGATGGC
    TTCCTGTGGG TCAAAAGTGG CTCAGTGACA ACCAAGGGCT CAGTTTGTAA CATAGCCCTC
    AGGTTTCAGA CTGTTCAGCC AATGGCTCTT CTACTTTTCC GAAGCAACAG GGATGTGTTT
    GTGAAGCTGG AGCTGCTAAG TGGCTACATT CACTTATCAA TTCAGGTCAA TAATCAGTCA
    AAGGTGCTTC TGTTCATTTC CCACAACACC AGCGATGGAG AGTGGCATTT CGTGGAGGTA
    ATATTTGCAG AGGCTGTGAC CCTTACCTTA ATCGACGACT CCTGTAAGGA GAAATGCATC
    GCGAAAGCTC CTACTCCACT TGAAAGTGAT CAATCAATAT GTGCTTTTCA GAACTCCTTT
    TTGGGTGGTT TACCAGTGGG AATGACCAGC AATGGTGTTG CTCTGCTTAA CTTCTATAAT
    ATGCCATCCA CACCTTCGTT TGTAGGCTGT CTCCAAGACA TTAAAATTGA TTGGAATCAC
    ATTACCCTGG AGAACATCTC GTCTGGCTCA TCATTAAATG TCAAGGCAGG CTGTGTGAGA
    AAGGATTGGT GTGAAAGCCA ACCTTGTCAA AGCAGAGGAC GCTGCATCAA CTTGTGGCTG
    AGTTACCAGT GTGACTGCCA CAGGCCCTAT GAAGGCCCCA ACTGTCTGAG AGAGTATGTG
    GCAGGCAGAT TTGGCCAGGA TGACTCCACT GGTTATGTCA TCTTTACTCT TGATGAGAGC
    TATGGAGACA CCATCAGCCT CTCCATGTTT GTCCGAACGC TTCAACCATC AGGCTTACTT
    CTAGCTTTGG AAAACAGCAC TTATCAATAT ATCCGTGTCT GGCTAGAGCG CGGCAGACTA
    GCAATGCTGA CTCCAAACTC TCCCAAATTA GTAGTAAAAT TTGTTCTTAA TGATGGAAAT
    GTCCACTTGA TATCTTTGAA AATCAAGCCA TATAAAATTG AACTGTATCA GTCTTCACAA
    AACCTAGGAT TTATTTCTGC TTCTACGTGG AAAATCGAAA AGGGAGATGT CATCTACATT
    GGTGGCCTAC CTGACAAGCA AGAGACTGAA CTTAATGGTG GATTCTTCAA AGGCTGTATC
    CAAGATGTAA GACTAAACAA CCAAAATCTG GAATTCTTTC CAAATCCAAC AAACAATGCA
    TCTCTCAATC CAGTTCTTGT CAATGTAACC CAAGGCTGTG CTGGAGACAA CAGCTGCAAG
    TCCAACCCCT GTCACAATGG AGGTGTTTGC CATTCCCGGT GGGATGACTT CTCCTGTTCC
    TGTCCTGCCC TCACAAGTGG GAAAGCCTGT GAGGAGGTTC AGTGGTGTGG ATTCAGCCCG
    TGTCCTCACG GAGCCCAGTG CCAGCCGGTG CTTCAAGGAT TTGAATGTAT TGCAAATGCT
    GTTTTTAATG GACAAAGCGG TCAAATATTA TTCAGAAGCA ATGGGAATAT TACCAGAGAA
    CTCACCAATA TCACATTTGG TTTCAGAACA AGGGATGCAA ATGTAATAAT ATTGCATGCA
    GAAAAAGAGC CTGAATTTCT TAATATTAGC ATTCAAGATT CCAGATTATT CTTTCAATTG
    CAAAGTGGCA ACAGCTTTTA TATGCTAAGT CTGACAAGTT TGCAGTCAGT GAATGATGGC
    ACATGGCACG AAGTGACCCT TTCCATGACA GACCCACTGT CCCAGACCTC CAGGTGGCAA
    ATGGAAGTGG ACAACGAAAC ACCTTTTGTG ACCAGCACAA TTGCTACTGG AAGCCTCAAC
    TTTTTGAAGG ATAATACAGA TATTTATGTG GGAGACAGAG CTATTGACAA TATAAAGGGC
    CTGCAAGGGT GTCTAAGTAC AATAGAAATC GGAGGCATTT ATCTCTCTTA CTTTGAAAAT
    GTTCATGGTT TCATTAATAA ACCTCAGGAA GAGCAATTTC TCAAAATCTC TACCAATTCA
    GTGGTCACTG GCTGTTTGCA GTTAAATGTC TGCAACTCCA ACCCCTGTTT GCATGGAGGA
    AACTGTGAAG ACATCTATAG CTCTTATCAT TGCTCCTGTC CCTTGGGATG GTCAGGGAAA
    CACTGTGAAC TCAACATCGA TGAATGCTTT TCAAACCCCT GTATCCATGG CAACTGCTCT
    GACAGAGTTG CAGCCTACCA CTGCACATGT GAGCCTGGAT ACACTGGTGT GAACTGTGAA
    GTGGATATAG ACAACTGCCA GAGTCACCAG TGTGCAAATG GAGCCACCTG CATTAGTCAT
    ACTAATGGCT ATTCTTGCCT CTGTTTTGGA AATTTTACAG GAAAATTTTG CAGACAGAGC
    AGATTACCCT CAACAGTCTG TGGGAATGAG AAGACAAATC TCACTTGCTA CAATGGAGGC
    AACTGCACAG AGTTCCAGAC TGAATTAAAA TGTATGTGCC GGCCAGGTTT TACTGGAGAA
    TGGTGTGAAA AGGACATTGA TGAGTGTGCC TCTGATCCGT GTGTCAATGG AGGTCTGTGC
    CAGGACTTAC TCAACAAATT CCAGTGCCTC TGTGATGTTG CCTTTGCTGG CGAGCGCTGC
    GAGGTGGACT TGGCAGATGA CTTGATCTCC GACATTTTCA CCACTATTGG CTCAGTGACT
    GTCGCCTTGT TACTGATCCT CTTGCTGGCC ATTGTTGCTT CTGTTGTCAC CTCCAACAAA
    AGGGCAACTC AGGGAACCTA CAGCCCCAGC CGTCAGGAGA AGGAGGGCTC CCGAGTGGAA
    ATGTGGAACT TGATGCCACC CCCTGCAATG GAGAGACTGA TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001180569.1
    CDS162..4046
    Translation

    Target ORF information:

    RefSeq Version NM_001193640.2
    Organism Homo sapiens(human)
    Definition Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001193640.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGATACACA
    GGTGCCCAGT GTGAGATCGA CCTCAATGAA TGCAATAGTA ACCCCTGCCA GTCCAATGGG
    GAATGTGTGG AGCTGTCCTC AGAGAAACAA TATGGACGCA TCACTGGACT GCCTTCTTCT
    TTCAGCTACC ATGAAGCCTC AGGTTATGTC TGTATCTGTC AGCCTGGATT CACAGGAATC
    CACTGCGAAG AAGACGTCAA TGAATGTTCT TCAAACCCTT GCCAAAATGG TGGTACTTGT
    GAGAACTTGC CTGGGAATTA TACTTGCCAT TGCCCATTTG ATAACCTTTC TAGAACTTTT
    TATGGAGGAA GGGACTGTTC TGATATTCTC CTGGGCTGTA CCCATCAGCA ATGTCTAAAT
    AATGGAACAT GCATCCCTCA CTTCCAAGAT GGCCAGCATG GATTCAGCTG CCTATGTCCA
    TCTGGCTACA CCGGGTCCCT GTGTGAAATC GCAACCACAC TTTCATTTGA GGGCGATGGC
    TTCCTGTGGG TCAAAAGTGG CTCAGTGACA ACCAAGGGCT CAGTTTGTAA CATAGCCCTC
    AGGTTTCAGA CTGTTCAGCC AATGGCTCTT CTACTTTTCC GAAGCAACAG GGATGTGTTT
    GTGAAGCTGG AGCTGCTAAG TGGCTACATT CACTTATCAA TTCAGGTCAA TAATCAGTCA
    AAGGTGCTTC TGTTCATTTC CCACAACACC AGCGATGGAG AGTGGCATTT CGTGGAGGTA
    ATATTTGCAG AGGCTGTGAC CCTTACCTTA ATCGACGACT CCTGTAAGGA GAAATGCATC
    GCGAAAGCTC CTACTCCACT TGAAAGTGAT CAATCAATAT GTGCTTTTCA GAACTCCTTT
    TTGGGTGGTT TACCAGTGGG AATGACCAGC AATGGTGTTG CTCTGCTTAA CTTCTATAAT
    ATGCCATCCA CACCTTCGTT TGTAGGCTGT CTCCAAGACA TTAAAATTGA TTGGAATCAC
    ATTACCCTGG AGAACATCTC GTCTGGCTCA TCATTAAATG TCAAGGCAGG CTGTGTGAGA
    AAGGATTGGT GTGAAAGCCA ACCTTGTCAA AGCAGAGGAC GCTGCATCAA CTTGTGGCTG
    AGTTACCAGT GTGACTGCCA CAGGCCCTAT GAAGGCCCCA ACTGTCTGAG AGAGTATGTG
    GCAGGCAGAT TTGGCCAGGA TGACTCCACT GGTTATGTCA TCTTTACTCT TGATGAGAGC
    TATGGAGACA CCATCAGCCT CTCCATGTTT GTCCGAACGC TTCAACCATC AGGCTTACTT
    CTAGCTTTGG AAAACAGCAC TTATCAATAT ATCCGTGTCT GGCTAGAGCG CGGCAGACTA
    GCAATGCTGA CTCCAAACTC TCCCAAATTA GTAGTAAAAT TTGTTCTTAA TGATGGAAAT
    GTCCACTTGA TATCTTTGAA AATCAAGCCA TATAAAATTG AACTGTATCA GTCTTCACAA
    AACCTAGGAT TTATTTCTGC TTCTACGTGG AAAATCGAAA AGGGAGATGT CATCTACATT
    GGTGGCCTAC CTGACAAGCA AGAGACTGAA CTTAATGGTG GATTCTTCAA AGGCTGTATC
    CAAGATGTAA GACTAAACAA CCAAAATCTG GAATTCTTTC CAAATCCAAC AAACAATGCA
    TCTCTCAATC CAGTTCTTGT CAATGTAACC CAAGGCTGTG CTGGAGACAA CAGCTGCAAG
    TCCAACCCCT GTCACAATGG AGGTGTTTGC CATTCCCGGT GGGATGACTT CTCCTGTTCC
    TGTCCTGCCC TCACAAGTGG GAAAGCCTGT GAGGAGGTTC AGTGGTGTGG ATTCAGCCCG
    TGTCCTCACG GAGCCCAGTG CCAGCCGGTG CTTCAAGGAT TTGAATGTAT TGCAAATGCT
    GTTTTTAATG GACAAAGCGG TCAAATATTA TTCAGAAGCA ATGGGAATAT TACCAGAGAA
    CTCACCAATA TCACATTTGG TTTCAGAACA AGGGATGCAA ATGTAATAAT ATTGCATGCA
    GAAAAAGAGC CTGAATTTCT TAATATTAGC ATTCAAGATT CCAGATTATT CTTTCAATTG
    CAAAGTGGCA ACAGCTTTTA TATGCTAAGT CTGACAAGTT TGCAGTCAGT GAATGATGGC
    ACATGGCACG AAGTGACCCT TTCCATGACA GACCCACTGT CCCAGACCTC CAGGTGGCAA
    ATGGAAGTGG ACAACGAAAC ACCTTTTGTG ACCAGCACAA TTGCTACTGG AAGCCTCAAC
    TTTTTGAAGG ATAATACAGA TATTTATGTG GGAGACAGAG CTATTGACAA TATAAAGGGC
    CTGCAAGGGT GTCTAAGTAC AATAGAAATC GGAGGCATTT ATCTCTCTTA CTTTGAAAAT
    GTTCATGGTT TCATTAATAA ACCTCAGGAA GAGCAATTTC TCAAAATCTC TACCAATTCA
    GTGGTCACTG GCTGTTTGCA GTTAAATGTC TGCAACTCCA ACCCCTGTTT GCATGGAGGA
    AACTGTGAAG ACATCTATAG CTCTTATCAT TGCTCCTGTC CCTTGGGATG GTCAGGGAAA
    CACTGTGAAC TCAACATCGA TGAATGCTTT TCAAACCCCT GTATCCATGG CAACTGCTCT
    GACAGAGTTG CAGCCTACCA CTGCACATGT GAGCCTGGAT ACACTGGTGT GAACTGTGAA
    GTGGATATAG ACAACTGCCA GAGTCACCAG TGTGCAAATG GAGCCACCTG CATTAGTCAT
    ACTAATGGCT ATTCTTGCCT CTGTTTTGGA AATTTTACAG GAAAATTTTG CAGACAGAGC
    AGATTACCCT CAACAGTCTG TGGGAATGAG AAGACAAATC TCACTTGCTA CAATGGAGGC
    AACTGCACAG AGTTCCAGAC TGAATTAAAA TGTATGTGCC GGCCAGGTTT TACTGGAGAA
    TGGTGTGAAA AGGACATTGA TGAGTGTGCC TCTGATCCGT GTGTCAATGG AGGTCTGTGC
    CAGGACTTAC TCAACAAATT CCAGTGCCTC TGTGATGTTG CCTTTGCTGG CGAGCGCTGC
    GAGGTGGACT TGGCAGATGA CTTGATCTCC GACATTTTCA CCACTATTGG CTCAGTGACT
    GTCGCCTTGT TACTGATCCT CTTGCTGGCC ATTGTTGCTT CTGTTGTCAC CTCCAACAAA
    AGGGCAACTC AGGGAACCTA CAGCCCCAGC CGTCAGGAGA AGGAGGGCTC CCGAGTGGAA
    ATGTGGAACT TGATGCCACC CCCTGCAATG GAGAGACTGA TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108193
    Clone ID Related Accession (Same CDS sequence) NM_001257965.2
    Accession Version NM_001257965.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product protein crumbs homolog 1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356315.12, AL513325.13, AL136322.24 and AL139136.17. On May 31, 2019 this sequence version replaced NM_001257965.1. Transcript Variant: This variant (3) contains three noncoding exons in place of the first exon and contains an alternate in-frame exon compared to variant 1. The resulting isoform (3) is shorter at the N- terminus and contains an alternate internal segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302826.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968832, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAAGACC CTTGCTTCTC CAATCCCTGT CAAGGAAGTG CCACTTGTGT GAACACCCCA 
    GGAGAAAGGA GCTTTCTGTG CAAATGTCCT CCTGGGTACA GTGGGACAAT CTGTGAAACT
    ACCATTGGTT CCTGTGGCAA GAACTCCTGC CAACATGGAG GTATTTGCCA TCAGGACCCT
    ATTTATCCTG TCTGCATCTG CCCTGCTGGA TATGCTGGAA GATTCTGTGA GATAGATCAC
    GATGAGTGTG CTTCCAGCCC TTGCCAAAAT GGGGCCGTGT GCCAGGATGG AATTGATGGT
    TACTCCTGCT TCTGTGTCCC AGGATATCAA GGCAGACACT GCGACTTGGA AGTGGATGAA
    TGTGCTTCAG ATCCCTGCAA GAACGAGGCT ACATGCCTCA ATGAAATAGG AAGATATACT
    TGTATCTGTC CCCACAATTA TTCTGGTGTA AACTGTGAAT TGGAAATTGA CGAATGTTGG
    TCCCAGCCTT GTTTAAATGG TGCAACTTGT CAGGATGCTC TGGGGGCCTA TTTCTGCGAC
    TGTGCCCCTG GATTCCTGGG GGATCACTGT GAACTCAACA CTGATGAGTG TGCCAGTCAA
    CCTTGTCTCC ATGGAGGGCT GTGTGTGGAT GGAGAAAACA GATATAGCTG TAACTGCACG
    GGTAGTGGAT TCACAGGGAC ACACTGTGAG ACCTTGATGC CTCTTTGTTG GTCAAAACCT
    TGTCACAATA ATGCTACATG TGAGGACAGT GTTGACAATT ACACTTGTCA CTGCTGGCCT
    GGATACACAG GTGCCCAGTG TGAGATCGAC CTCAATGAAT GCAATAGTAA CCCCTGCCAG
    TCCAATGGGG AATGTGTGGA GCTGTCCTCA GAGAAACAAT ATGGACGCAT CACTGGACTG
    CCTTCTTCTT TCAGCTACCA TGAAGCCTCA GGTTATGTCT GTATCTGTCA GCCTGGATTC
    ACAGGAATCC ACTGCGAAGA AGACGTCAAT GAATGTTCTT CAAACCCTTG CCAAAATGGT
    GGTACTTGTG AGAACTTGCC TGGGAATTAT ACTTGCCATT GCCCATTTGA TAACCTTTCT
    AGAACTTTTT ATGGAGGAAG GGACTGTTCT GATATTCTCC TGGGCTGTAC CCATCAGCAA
    TGTCTAAATA ATGGAACATG CATCCCTCAC TTCCAAGATG GCCAGCATGG ATTCAGCTGC
    CTATGTCCAT CTGGCTACAC CGGGTCCCTG TGTGAAATCG CAACCACACT TTCATTTGAG
    GGCGATGGCT TCCTGTGGGT CAAAAGTGGC TCAGTGACAA CCAAGGGCTC AGTTTGTAAC
    ATAGCCCTCA GGTTTCAGAC TGTTCAGCCA ATGGCTCTTC TACTTTTCCG AAGCAACAGG
    GATGTGTTTG TGAAGCTGGA GCTGCTAAGT GGCTACATTC ACTTATCAAT TCAGGTCAAT
    AATCAGTCAA AGGTGCTTCT GTTCATTTCC CACAACACCA GCGATGGAGA GTGGCATTTC
    GTGGAGGTAA TATTTGCAGA GGCTGTGACC CTTACCTTAA TCGACGACTC CTGTAAGGAG
    AAATGCATCG CGAAAGCTCC TACTCCACTT GAAAGTGATC AATCAATATG TGCTTTTCAG
    AACTCCTTTT TGGGTGGTTT ACCAGTGGGA ATGACCAGCA ATGGTGTTGC TCTGCTTAAC
    TTCTATAATA TGCCATCCAC ACCTTCGTTT GTAGGCTGTC TCCAAGACAT TAAAATTGAT
    TGGAATCACA TTACCCTGGA GAACATCTCG TCTGGCTCAT CATTAAATGT CAAGGCAGGC
    TGTGTGAGAA AGGATTGGTG TGAAAGCCAA CCTTGTCAAA GCAGAGGACG CTGCATCAAC
    TTGTGGCTGA GTTACCAGTG TGACTGCCAC AGGCCCTATG AAGGCCCCAA CTGTCTGAGA
    GAGTATGTGG CAGGCAGATT TGGCCAGGAT GACTCCACTG GTTATGTCAT CTTTACTCTT
    GATGAGAGCT ATGGAGACAC CATCAGCCTC TCCATGTTTG TCCGAACGCT TCAACCATCA
    GGCTTACTTC TAGCTTTGGA AAACAGCACT TATCAATATA TCCGTGTCTG GCTAGAGCGC
    GGCAGACTAG CAATGCTGAC TCCAAACTCT CCCAAATTAG TAGTAAAATT TGTTCTTAAT
    GATGGAAATG TCCACTTGAT ATCTTTGAAA ATCAAGCCAT ATAAAATTGA ACTGTATCAG
    TCTTCACAAA ACCTAGGATT TATTTCTGCT TCTACGTGGA AAATCGAAAA GGGAGATGTC
    ATCTACATTG GTGGCCTACC TGACAAGCAA GAGACTGAAC TTAATGGTGG ATTCTTCAAA
    GGCTGTATCC AAGATGTAAG ACTAAACAAC CAAAATCTGG AATTCTTTCC AAATCCAACA
    AACAATGCAT CTCTCAATCC AGTTCTTGTC AATGTAACCC AAGGCTGTGC TGGAGACAAC
    AGCTGCAAGA GGCAGACCAA TGTGGGAAGG GCACTCACTG AGTTGGGATC CAGAGGACCT
    AAGTACCAAG TTTCACTGTT TCGCTTCTGT GTAGGATCTT GGGCAACTGG AAACACCTTC
    TTTTTATCAT CTATAAAACC AGGATCCAAC CCCTGTCACA ATGGAGGTGT TTGCCATTCC
    CGGTGGGATG ACTTCTCCTG TTCCTGTCCT GCCCTCACAA GTGGGAAAGC CTGTGAGGAG
    GTTCAGTGGT GTGGATTCAG CCCGTGTCCT CACGGAGCCC AGTGCCAGCC GGTGCTTCAA
    GGATTTGAAT GTATTGCAAA TGCTGTTTTT AATGGACAAA GCGGTCAAAT ATTATTCAGA
    AGCAATGGGA ATATTACCAG AGAACTCACC AATATCACAT TTGGTTTCAG AACAAGGGAT
    GCAAATGTAA TAATATTGCA TGCAGAAAAA GAGCCTGAAT TTCTTAATAT TAGCATTCAA
    GATTCCAGAT TATTCTTTCA ATTGCAAAGT GGCAACAGCT TTTATATGCT AAGTCTGACA
    AGTTTGCAGT CAGTGAATGA TGGCACATGG CACGAAGTGA CCCTTTCCAT GACAGACCCA
    CTGTCCCAGA CCTCCAGGTG GCAAATGGAA GTGGACAACG AAACACCTTT TGTGACCAGC
    ACAATTGCTA CTGGAAGCCT CAACTTTTTG AAGGATAATA CAGATATTTA TGTGGGAGAC
    AGAGCTATTG ACAATATAAA GGGCCTGCAA GGGTGTCTAA GTACAATAGA AATCGGAGGC
    ATTTATCTCT CTTACTTTGA AAATGTTCAT GGTTTCATTA ATAAACCTCA GGAAGAGCAA
    TTTCTCAAAA TCTCTACCAA TTCAGTGGTC ACTGGCTGTT TGCAGTTAAA TGTCTGCAAC
    TCCAACCCCT GTTTGCATGG AGGAAACTGT GAAGACATCT ATAGCTCTTA TCATTGCTCC
    TGTCCCTTGG GATGGTCAGG GAAACACTGT GAACTCAACA TCGATGAATG CTTTTCAAAC
    CCCTGTATCC ATGGCAACTG CTCTGACAGA GTTGCAGCCT ACCACTGCAC ATGTGAGCCT
    GGATACACTG GTGTGAACTG TGAAGTGGAT ATAGACAACT GCCAGAGTCA CCAGTGTGCA
    AATGGAGCCA CCTGCATTAG TCATACTAAT GGCTATTCTT GCCTCTGTTT TGGAAATTTT
    ACAGGAAAAT TTTGCAGACA GAGCAGATTA CCCTCAACAG TCTGTGGGAA TGAGAAGACA
    AATCTCACTT GCTACAATGG AGGCAACTGC ACAGAGTTCC AGACTGAATT AAAATGTATG
    TGCCGGCCAG GTTTTACTGG AGAATGGTGT GAAAAGGACA TTGATGAGTG TGCCTCTGAT
    CCGTGTGTCA ATGGAGGTCT GTGCCAGGAC TTACTCAACA AATTCCAGTG CCTCTGTGAT
    GTTGCCTTTG CTGGCGAGCG CTGCGAGGTG GACTTGGCAG ATGACTTGAT CTCCGACATT
    TTCACCACTA TTGGCTCAGT GACTGTCGCC TTGTTACTGA TCCTCTTGCT GGCCATTGTT
    GCTTCTGTTG TCACCTCCAA CAAAAGGGCA ACTCAGGGAA CCTACAGCCC CAGCCGTCAG
    GAGAAGGAGG GCTCCCGAGT GGAAATGTGG AACTTGATGC CACCCCCTGC AATGGAGAGA
    CTGATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001244894.1
    CDS298..4446
    Translation

    Target ORF information:

    RefSeq Version NM_001257965.2
    Organism Homo sapiens(human)
    Definition Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001257965.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAAGACC CTTGCTTCTC CAATCCCTGT CAAGGAAGTG CCACTTGTGT GAACACCCCA 
    GGAGAAAGGA GCTTTCTGTG CAAATGTCCT CCTGGGTACA GTGGGACAAT CTGTGAAACT
    ACCATTGGTT CCTGTGGCAA GAACTCCTGC CAACATGGAG GTATTTGCCA TCAGGACCCT
    ATTTATCCTG TCTGCATCTG CCCTGCTGGA TATGCTGGAA GATTCTGTGA GATAGATCAC
    GATGAGTGTG CTTCCAGCCC TTGCCAAAAT GGGGCCGTGT GCCAGGATGG AATTGATGGT
    TACTCCTGCT TCTGTGTCCC AGGATATCAA GGCAGACACT GCGACTTGGA AGTGGATGAA
    TGTGCTTCAG ATCCCTGCAA GAACGAGGCT ACATGCCTCA ATGAAATAGG AAGATATACT
    TGTATCTGTC CCCACAATTA TTCTGGTGTA AACTGTGAAT TGGAAATTGA CGAATGTTGG
    TCCCAGCCTT GTTTAAATGG TGCAACTTGT CAGGATGCTC TGGGGGCCTA TTTCTGCGAC
    TGTGCCCCTG GATTCCTGGG GGATCACTGT GAACTCAACA CTGATGAGTG TGCCAGTCAA
    CCTTGTCTCC ATGGAGGGCT GTGTGTGGAT GGAGAAAACA GATATAGCTG TAACTGCACG
    GGTAGTGGAT TCACAGGGAC ACACTGTGAG ACCTTGATGC CTCTTTGTTG GTCAAAACCT
    TGTCACAATA ATGCTACATG TGAGGACAGT GTTGACAATT ACACTTGTCA CTGCTGGCCT
    GGATACACAG GTGCCCAGTG TGAGATCGAC CTCAATGAAT GCAATAGTAA CCCCTGCCAG
    TCCAATGGGG AATGTGTGGA GCTGTCCTCA GAGAAACAAT ATGGACGCAT CACTGGACTG
    CCTTCTTCTT TCAGCTACCA TGAAGCCTCA GGTTATGTCT GTATCTGTCA GCCTGGATTC
    ACAGGAATCC ACTGCGAAGA AGACGTCAAT GAATGTTCTT CAAACCCTTG CCAAAATGGT
    GGTACTTGTG AGAACTTGCC TGGGAATTAT ACTTGCCATT GCCCATTTGA TAACCTTTCT
    AGAACTTTTT ATGGAGGAAG GGACTGTTCT GATATTCTCC TGGGCTGTAC CCATCAGCAA
    TGTCTAAATA ATGGAACATG CATCCCTCAC TTCCAAGATG GCCAGCATGG ATTCAGCTGC
    CTATGTCCAT CTGGCTACAC CGGGTCCCTG TGTGAAATCG CAACCACACT TTCATTTGAG
    GGCGATGGCT TCCTGTGGGT CAAAAGTGGC TCAGTGACAA CCAAGGGCTC AGTTTGTAAC
    ATAGCCCTCA GGTTTCAGAC TGTTCAGCCA ATGGCTCTTC TACTTTTCCG AAGCAACAGG
    GATGTGTTTG TGAAGCTGGA GCTGCTAAGT GGCTACATTC ACTTATCAAT TCAGGTCAAT
    AATCAGTCAA AGGTGCTTCT GTTCATTTCC CACAACACCA GCGATGGAGA GTGGCATTTC
    GTGGAGGTAA TATTTGCAGA GGCTGTGACC CTTACCTTAA TCGACGACTC CTGTAAGGAG
    AAATGCATCG CGAAAGCTCC TACTCCACTT GAAAGTGATC AATCAATATG TGCTTTTCAG
    AACTCCTTTT TGGGTGGTTT ACCAGTGGGA ATGACCAGCA ATGGTGTTGC TCTGCTTAAC
    TTCTATAATA TGCCATCCAC ACCTTCGTTT GTAGGCTGTC TCCAAGACAT TAAAATTGAT
    TGGAATCACA TTACCCTGGA GAACATCTCG TCTGGCTCAT CATTAAATGT CAAGGCAGGC
    TGTGTGAGAA AGGATTGGTG TGAAAGCCAA CCTTGTCAAA GCAGAGGACG CTGCATCAAC
    TTGTGGCTGA GTTACCAGTG TGACTGCCAC AGGCCCTATG AAGGCCCCAA CTGTCTGAGA
    GAGTATGTGG CAGGCAGATT TGGCCAGGAT GACTCCACTG GTTATGTCAT CTTTACTCTT
    GATGAGAGCT ATGGAGACAC CATCAGCCTC TCCATGTTTG TCCGAACGCT TCAACCATCA
    GGCTTACTTC TAGCTTTGGA AAACAGCACT TATCAATATA TCCGTGTCTG GCTAGAGCGC
    GGCAGACTAG CAATGCTGAC TCCAAACTCT CCCAAATTAG TAGTAAAATT TGTTCTTAAT
    GATGGAAATG TCCACTTGAT ATCTTTGAAA ATCAAGCCAT ATAAAATTGA ACTGTATCAG
    TCTTCACAAA ACCTAGGATT TATTTCTGCT TCTACGTGGA AAATCGAAAA GGGAGATGTC
    ATCTACATTG GTGGCCTACC TGACAAGCAA GAGACTGAAC TTAATGGTGG ATTCTTCAAA
    GGCTGTATCC AAGATGTAAG ACTAAACAAC CAAAATCTGG AATTCTTTCC AAATCCAACA
    AACAATGCAT CTCTCAATCC AGTTCTTGTC AATGTAACCC AAGGCTGTGC TGGAGACAAC
    AGCTGCAAGA GGCAGACCAA TGTGGGAAGG GCACTCACTG AGTTGGGATC CAGAGGACCT
    AAGTACCAAG TTTCACTGTT TCGCTTCTGT GTAGGATCTT GGGCAACTGG AAACACCTTC
    TTTTTATCAT CTATAAAACC AGGATCCAAC CCCTGTCACA ATGGAGGTGT TTGCCATTCC
    CGGTGGGATG ACTTCTCCTG TTCCTGTCCT GCCCTCACAA GTGGGAAAGC CTGTGAGGAG
    GTTCAGTGGT GTGGATTCAG CCCGTGTCCT CACGGAGCCC AGTGCCAGCC GGTGCTTCAA
    GGATTTGAAT GTATTGCAAA TGCTGTTTTT AATGGACAAA GCGGTCAAAT ATTATTCAGA
    AGCAATGGGA ATATTACCAG AGAACTCACC AATATCACAT TTGGTTTCAG AACAAGGGAT
    GCAAATGTAA TAATATTGCA TGCAGAAAAA GAGCCTGAAT TTCTTAATAT TAGCATTCAA
    GATTCCAGAT TATTCTTTCA ATTGCAAAGT GGCAACAGCT TTTATATGCT AAGTCTGACA
    AGTTTGCAGT CAGTGAATGA TGGCACATGG CACGAAGTGA CCCTTTCCAT GACAGACCCA
    CTGTCCCAGA CCTCCAGGTG GCAAATGGAA GTGGACAACG AAACACCTTT TGTGACCAGC
    ACAATTGCTA CTGGAAGCCT CAACTTTTTG AAGGATAATA CAGATATTTA TGTGGGAGAC
    AGAGCTATTG ACAATATAAA GGGCCTGCAA GGGTGTCTAA GTACAATAGA AATCGGAGGC
    ATTTATCTCT CTTACTTTGA AAATGTTCAT GGTTTCATTA ATAAACCTCA GGAAGAGCAA
    TTTCTCAAAA TCTCTACCAA TTCAGTGGTC ACTGGCTGTT TGCAGTTAAA TGTCTGCAAC
    TCCAACCCCT GTTTGCATGG AGGAAACTGT GAAGACATCT ATAGCTCTTA TCATTGCTCC
    TGTCCCTTGG GATGGTCAGG GAAACACTGT GAACTCAACA TCGATGAATG CTTTTCAAAC
    CCCTGTATCC ATGGCAACTG CTCTGACAGA GTTGCAGCCT ACCACTGCAC ATGTGAGCCT
    GGATACACTG GTGTGAACTG TGAAGTGGAT ATAGACAACT GCCAGAGTCA CCAGTGTGCA
    AATGGAGCCA CCTGCATTAG TCATACTAAT GGCTATTCTT GCCTCTGTTT TGGAAATTTT
    ACAGGAAAAT TTTGCAGACA GAGCAGATTA CCCTCAACAG TCTGTGGGAA TGAGAAGACA
    AATCTCACTT GCTACAATGG AGGCAACTGC ACAGAGTTCC AGACTGAATT AAAATGTATG
    TGCCGGCCAG GTTTTACTGG AGAATGGTGT GAAAAGGACA TTGATGAGTG TGCCTCTGAT
    CCGTGTGTCA ATGGAGGTCT GTGCCAGGAC TTACTCAACA AATTCCAGTG CCTCTGTGAT
    GTTGCCTTTG CTGGCGAGCG CTGCGAGGTG GACTTGGCAG ATGACTTGAT CTCCGACATT
    TTCACCACTA TTGGCTCAGT GACTGTCGCC TTGTTACTGA TCCTCTTGCT GGCCATTGTT
    GCTTCTGTTG TCACCTCCAA CAAAAGGGCA ACTCAGGGAA CCTACAGCCC CAGCCGTCAG
    GAGAAGGAGG GCTCCCGAGT GGAAATGTGG AACTTGATGC CACCCCCTGC AATGGAGAGA
    CTGATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu87140
    Clone ID Related Accession (Same CDS sequence) XM_017000851.1
    Accession Version XM_017000851.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3378bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein crumbs homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTGCAAC TTGTCAGGAT GCTCTGGGGG CCTATTTCTG CGACTGTGCC CCTGGATTCC 
    TGGGGGATCA CTGTGAACTC AACACTGATG AGTGTGCCAG TCAACCTTGT CTCCATGGAG
    GGCTGTGTGT GGATGGAGAA AACAGGATAC ACAGGTGCCC AGTGTGAGAT CGACCTCAAT
    GAATGCAATA GTAACCCCTG CCAGTCCAAT GGGGAATGTG TGGAGCTGTC CTCAGAGAAA
    CAATATGGAC GCATCACTGG ACTGCCTTCT TCTTTCAGCT ACCATGAAGC CTCAGGTTAT
    GTCTGTATCT GTCAGCCTGG ATTCACAGGA ATCCACTGCG AAGAAGACGT CAATGAATGT
    TCTTCAAACC CTTGCCAAAA TGGTGGTACT TGTGAGAACT TGCCTGGGAA TTATACTTGC
    CATTGCCCAT TTGATAACCT TTCTAGAACT TTTTATGGAG GAAGGGACTG TTCTGATATT
    CTCCTGGGCT GTACCCATCA GCAATGTCTA AATAATGGAA CATGCATCCC TCACTTCCAA
    GATGGCCAGC ATGGATTCAG CTGCCTATGT CCATCTGGCT ACACCGGGTC CCTGTGTGAA
    ATCGCAACCA CACTTTCATT TGAGGGCGAT GGCTTCCTGT GGGTCAAAAG TGGCTCAGTG
    ACAACCAAGG GCTCAGTTTG TAACATAGCC CTCAGGTTTC AGACTGTTCA GCCAATGGCT
    CTTCTACTTT TCCGAAGCAA CAGGGATGTG TTTGTGAAGC TGGAGCTGCT AAGTGGCTAC
    ATTCACTTAT CAATTCAGGT CAATAATCAG TCAAAGGTGC TTCTGTTCAT TTCCCACAAC
    ACCAGCGATG GAGAGTGGCA TTTCGTGGAG GTAATATTTG CAGAGGCTGT GACCCTTACC
    TTAATCGACG ACTCCTGTAA GGAGAAATGC ATCGCGAAAG CTCCTACTCC ACTTGAAAGT
    GATCAATCAA TATGTGCTTT TCAGAACTCC TTTTTGGGTG GTTTACCAGT GGGAATGACC
    AGCAATGGTG TTGCTCTGCT TAACTTCTAT AATATGCCAT CCACACCTTC GTTTGTAGGC
    TGTCTCCAAG ACATTAAAAT TGATTGGAAT CACATTACCC TGGAGAACAT CTCGTCTGGC
    TCATCATTAA ATGTCAAGGC AGGCTGTGTG AGAAAGGATT GGTGTGAAAG CCAACCTTGT
    CAAAGCAGAG GACGCTGCAT CAACTTGTGG CTGAGTTACC AGTGTGACTG CCACAGGCCC
    TATGAAGGCC CCAACTGTCT GAGAGAGTAT GTGGCAGGCA GATTTGGCCA GGATGACTCC
    ACTGGTTATG TCATCTTTAC TCTTGATGAG AGCTATGGAG ACACCATCAG CCTCTCCATG
    TTTGTCCGAA CGCTTCAACC ATCAGGCTTA CTTCTAGCTT TGGAAAACAG CACTTATCAA
    TATATCCGTG TCTGGCTAGA GCGCGGCAGA CTAGCAATGC TGACTCCAAA CTCTCCCAAA
    TTAGTAGTAA AATTTGTTCT TAATGATGGA AATGTCCACT TGATATCTTT GAAAATCAAG
    CCATATAAAA TTGAACTGTA TCAGTCTTCA CAAAACCTAG GATTTATTTC TGCTTCTACG
    TGGAAAATCG AAAAGGGAGA TGTCATCTAC ATTGGTGGCC TACCTGACAA GCAAGAGACT
    GAACTTAATG GTGGATTCTT CAAAGGCTGT ATCCAAGATG TAAGACTAAA CAACCAAAAT
    CTGGAATTCT TTCCAAATCC AACAAACAAT GCATCTCTCA ATCCAGTTCT TGTCAATGTA
    ACCCAAGGCT GTGCTGGAGA CAACAGCTGC AAGTCCAACC CCTGTCACAA TGGAGGTGTT
    TGCCATTCCC GGTGGGATGA CTTCTCCTGT TCCTGTCCTG CCCTCACAAG TGGGAAAGCC
    TGTGAGGAGG TTCAGTGGTG TGGATTCAGC CCGTGTCCTC ACGGAGCCCA GTGCCAGCCG
    GTGCTTCAAG GATTTGAATG TATTGCAAAT GCTGTTTTTA ATGGACAAAG CGGTCAAATA
    TTATTCAGAA GCAATGGGAA TATTACCAGA GAACTCACCA ATATCACATT TGGTTTCAGA
    ACAAGGGATG CAAATGTAAT AATATTGCAT GCAGAAAAAG AGCCTGAATT TCTTAATATT
    AGCATTCAAG ATTCCAGATT ATTCTTTCAA TTGCAAAGTG GCAACAGCTT TTATATGCTA
    AGTCTGACAA GTTTGCAGTC AGTGAATGAT GGCACATGGC ACGAAGTGAC CCTTTCCATG
    ACAGACCCAC TGTCCCAGAC CTCCAGGTGG CAAATGGAAG TGGACAACGA AACACCTTTT
    GTGACCAGCA CAATTGCTAC TGGAAGCCTC AACTTTTTGA AGGATAATAC AGATATTTAT
    GTGGGAGACA GAGCTATTGA CAATATAAAG GGCCTGCAAG GGTGTCTAAG TACAATAGAA
    ATCGGAGGCA TTTATCTCTC TTACTTTGAA AATGTTCATG GTTTCATTAA TAAACCTCAG
    GAAGAGCAAT TTCTCAAAAT CTCTACCAAT TCAGTGGTCA CTGGCTGTTT GCAGTTAAAT
    GTCTGCAACT CCAACCCCTG TTTGCATGGA GGAAACTGTG AAGACATCTA TAGCTCTTAT
    CATTGCTCCT GTCCCTTGGG ATGGTCAGGG AAACACTGTG AACTCAACAT CGATGAATGC
    TTTTCAAACC CCTGTATCCA TGGCAACTGC TCTGACAGAG TTGCAGCCTA CCACTGCACA
    TGTGAGCCTG GATACACTGG TGTGAACTGT GAAGTGGATA TAGACAACTG CCAGAGTCAC
    CAGTGTGCAA ATGGAGCCAC CTGCATTAGT CATACTAATG GCTATTCTTG CCTCTGTTTT
    GGAAATTTTA CAGGAAAATT TTGCAGACAG AGCAGATTAC CCTCAACAGT CTGTGGGAAT
    GAGAAGACAA ATCTCACTTG CTACAATGGA GGCAACTGCA CAGAGTTCCA GACTGAATTA
    AAATGTATGT GCCGGCCAGG TTTTACTGGA GAATGGTGTG AAAAGGACAT TGATGAGTGT
    GCCTCTGATC CGTGTGTCAA TGGAGGTCTG TGCCAGGACT TACTCAACAA ATTCCAGTGC
    CTCTGTGATG TTGCCTTTGC TGGCGAGCGC TGCGAGGTGG ACTTGGCAGA TGACTTGATC
    TCCGACATTT TCACCACTAT TGGCTCAGTG ACTGTCGCCT TGTTACTGAT CCTCTTGCTG
    GCCATTGTTG CTTCTGTTGT CACCTCCAAC AAAAGGGCAA CTCAGGGAAC CTACAGCCCC
    AGCCGTCAGG AGAAGGAGGG CTCCCGAGTG GAAATGTGGA ACTTGATGCC ACCCCCTGCA
    ATGGAGAGAC TGATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016856340.1
    CDS745..4122
    Translation

    Target ORF information:

    RefSeq Version XM_017000851.1
    Organism Homo sapiens(human)
    Definition Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017000851.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTGCAAC TTGTCAGGAT GCTCTGGGGG CCTATTTCTG CGACTGTGCC CCTGGATTCC 
    TGGGGGATCA CTGTGAACTC AACACTGATG AGTGTGCCAG TCAACCTTGT CTCCATGGAG
    GGCTGTGTGT GGATGGAGAA AACAGGATAC ACAGGTGCCC AGTGTGAGAT CGACCTCAAT
    GAATGCAATA GTAACCCCTG CCAGTCCAAT GGGGAATGTG TGGAGCTGTC CTCAGAGAAA
    CAATATGGAC GCATCACTGG ACTGCCTTCT TCTTTCAGCT ACCATGAAGC CTCAGGTTAT
    GTCTGTATCT GTCAGCCTGG ATTCACAGGA ATCCACTGCG AAGAAGACGT CAATGAATGT
    TCTTCAAACC CTTGCCAAAA TGGTGGTACT TGTGAGAACT TGCCTGGGAA TTATACTTGC
    CATTGCCCAT TTGATAACCT TTCTAGAACT TTTTATGGAG GAAGGGACTG TTCTGATATT
    CTCCTGGGCT GTACCCATCA GCAATGTCTA AATAATGGAA CATGCATCCC TCACTTCCAA
    GATGGCCAGC ATGGATTCAG CTGCCTATGT CCATCTGGCT ACACCGGGTC CCTGTGTGAA
    ATCGCAACCA CACTTTCATT TGAGGGCGAT GGCTTCCTGT GGGTCAAAAG TGGCTCAGTG
    ACAACCAAGG GCTCAGTTTG TAACATAGCC CTCAGGTTTC AGACTGTTCA GCCAATGGCT
    CTTCTACTTT TCCGAAGCAA CAGGGATGTG TTTGTGAAGC TGGAGCTGCT AAGTGGCTAC
    ATTCACTTAT CAATTCAGGT CAATAATCAG TCAAAGGTGC TTCTGTTCAT TTCCCACAAC
    ACCAGCGATG GAGAGTGGCA TTTCGTGGAG GTAATATTTG CAGAGGCTGT GACCCTTACC
    TTAATCGACG ACTCCTGTAA GGAGAAATGC ATCGCGAAAG CTCCTACTCC ACTTGAAAGT
    GATCAATCAA TATGTGCTTT TCAGAACTCC TTTTTGGGTG GTTTACCAGT GGGAATGACC
    AGCAATGGTG TTGCTCTGCT TAACTTCTAT AATATGCCAT CCACACCTTC GTTTGTAGGC
    TGTCTCCAAG ACATTAAAAT TGATTGGAAT CACATTACCC TGGAGAACAT CTCGTCTGGC
    TCATCATTAA ATGTCAAGGC AGGCTGTGTG AGAAAGGATT GGTGTGAAAG CCAACCTTGT
    CAAAGCAGAG GACGCTGCAT CAACTTGTGG CTGAGTTACC AGTGTGACTG CCACAGGCCC
    TATGAAGGCC CCAACTGTCT GAGAGAGTAT GTGGCAGGCA GATTTGGCCA GGATGACTCC
    ACTGGTTATG TCATCTTTAC TCTTGATGAG AGCTATGGAG ACACCATCAG CCTCTCCATG
    TTTGTCCGAA CGCTTCAACC ATCAGGCTTA CTTCTAGCTT TGGAAAACAG CACTTATCAA
    TATATCCGTG TCTGGCTAGA GCGCGGCAGA CTAGCAATGC TGACTCCAAA CTCTCCCAAA
    TTAGTAGTAA AATTTGTTCT TAATGATGGA AATGTCCACT TGATATCTTT GAAAATCAAG
    CCATATAAAA TTGAACTGTA TCAGTCTTCA CAAAACCTAG GATTTATTTC TGCTTCTACG
    TGGAAAATCG AAAAGGGAGA TGTCATCTAC ATTGGTGGCC TACCTGACAA GCAAGAGACT
    GAACTTAATG GTGGATTCTT CAAAGGCTGT ATCCAAGATG TAAGACTAAA CAACCAAAAT
    CTGGAATTCT TTCCAAATCC AACAAACAAT GCATCTCTCA ATCCAGTTCT TGTCAATGTA
    ACCCAAGGCT GTGCTGGAGA CAACAGCTGC AAGTCCAACC CCTGTCACAA TGGAGGTGTT
    TGCCATTCCC GGTGGGATGA CTTCTCCTGT TCCTGTCCTG CCCTCACAAG TGGGAAAGCC
    TGTGAGGAGG TTCAGTGGTG TGGATTCAGC CCGTGTCCTC ACGGAGCCCA GTGCCAGCCG
    GTGCTTCAAG GATTTGAATG TATTGCAAAT GCTGTTTTTA ATGGACAAAG CGGTCAAATA
    TTATTCAGAA GCAATGGGAA TATTACCAGA GAACTCACCA ATATCACATT TGGTTTCAGA
    ACAAGGGATG CAAATGTAAT AATATTGCAT GCAGAAAAAG AGCCTGAATT TCTTAATATT
    AGCATTCAAG ATTCCAGATT ATTCTTTCAA TTGCAAAGTG GCAACAGCTT TTATATGCTA
    AGTCTGACAA GTTTGCAGTC AGTGAATGAT GGCACATGGC ACGAAGTGAC CCTTTCCATG
    ACAGACCCAC TGTCCCAGAC CTCCAGGTGG CAAATGGAAG TGGACAACGA AACACCTTTT
    GTGACCAGCA CAATTGCTAC TGGAAGCCTC AACTTTTTGA AGGATAATAC AGATATTTAT
    GTGGGAGACA GAGCTATTGA CAATATAAAG GGCCTGCAAG GGTGTCTAAG TACAATAGAA
    ATCGGAGGCA TTTATCTCTC TTACTTTGAA AATGTTCATG GTTTCATTAA TAAACCTCAG
    GAAGAGCAAT TTCTCAAAAT CTCTACCAAT TCAGTGGTCA CTGGCTGTTT GCAGTTAAAT
    GTCTGCAACT CCAACCCCTG TTTGCATGGA GGAAACTGTG AAGACATCTA TAGCTCTTAT
    CATTGCTCCT GTCCCTTGGG ATGGTCAGGG AAACACTGTG AACTCAACAT CGATGAATGC
    TTTTCAAACC CCTGTATCCA TGGCAACTGC TCTGACAGAG TTGCAGCCTA CCACTGCACA
    TGTGAGCCTG GATACACTGG TGTGAACTGT GAAGTGGATA TAGACAACTG CCAGAGTCAC
    CAGTGTGCAA ATGGAGCCAC CTGCATTAGT CATACTAATG GCTATTCTTG CCTCTGTTTT
    GGAAATTTTA CAGGAAAATT TTGCAGACAG AGCAGATTAC CCTCAACAGT CTGTGGGAAT
    GAGAAGACAA ATCTCACTTG CTACAATGGA GGCAACTGCA CAGAGTTCCA GACTGAATTA
    AAATGTATGT GCCGGCCAGG TTTTACTGGA GAATGGTGTG AAAAGGACAT TGATGAGTGT
    GCCTCTGATC CGTGTGTCAA TGGAGGTCTG TGCCAGGACT TACTCAACAA ATTCCAGTGC
    CTCTGTGATG TTGCCTTTGC TGGCGAGCGC TGCGAGGTGG ACTTGGCAGA TGACTTGATC
    TCCGACATTT TCACCACTAT TGGCTCAGTG ACTGTCGCCT TGTTACTGAT CCTCTTGCTG
    GCCATTGTTG CTTCTGTTGT CACCTCCAAC AAAAGGGCAA CTCAGGGAAC CTACAGCCCC
    AGCCGTCAGG AGAAGGAGGG CTCCCGAGTG GAAATGTGGA ACTTGATGCC ACCCCCTGCA
    ATGGAGAGAC TGATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu87141
    Clone ID Related Accession (Same CDS sequence) XM_017000852.1
    Accession Version XM_017000852.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein crumbs homolog 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC
    TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG
    GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA
    CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA
    GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC
    TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT
    GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC
    AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG
    AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT
    TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA
    TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT
    TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT
    ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC
    CAAGATGGCC AGCATGGATT CAGCTGCCTA TGTCCATCTG GCTACACCGG GTCCCTGTGT
    GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA
    GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG
    GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC
    TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC
    AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT
    ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA
    AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG
    ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA
    GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT
    GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT
    TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG
    CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC
    TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC
    ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT
    CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC
    AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC
    AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT
    ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG
    ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA
    AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT
    GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGAGGC AGACCAATGT GGGAAGGGCA
    CTCACTGAGT TGGGATCCAG AGGACCTAAG TACCAAGTTT CACTGTTTCG CTTCTGTGTA
    GGATCTTGGG CAACTGGAAA CACCTTCTTT TTATCATCTA TAAAACCAGG ATCCAACCCC
    TGTCACAATG GAGGTGTTTG CCATTCCCGG TGGGATGACT TCTCCTGTTC CTGTCCTGCC
    CTCACAAGTG GGAAAGCCTG TGAGGAGGTT CAGTGGTGTG GATTCAGCCC GTGTCCTCAC
    GGAGCCCAGT GCCAGCCGGT GCTTCAAGGA TTTGAATGTA TTGCAAATGC TGTTTTTAAT
    GGACAAAGCG GTCAAATATT ATTCAGAAGC AATGGGAATA TTACCAGAGA ACTCACCAAT
    ATCACATTTG GTTTCAGAAC AAGGGATGCA AATGTAATAA TATTGCATGC AGAAAAAGAG
    CCTGAATTTC TTAATATTAG CATTCAAGAT TCCAGATTAT TCTTTCAATT GCAAAGTGGC
    AACAGCTTTT ATATGCTAAG TCTGACAAGT TTGCAGTCAG TGAATGATGG CACATGGCAC
    GAAGTGACCC TTTCCATGAC AGACCCACTG TCCCAGACCT CCAGGTGGCA AATGGAAGTG
    GACAACGAAA CACCTTTTGT GACCAGCACA ATTGCTACTG GAAGCCTCAA CTTTTTGAAG
    GATAATACAG ATATTTATGT GGGAGACAGA GCTATTGACA ATATAAAGGG CCTGCAAGGG
    TGTCTAAGTA CAATAGAAAT CGGAGGCATT TATCTCTCTT ACTTTGAAAA TGTTCATGGT
    TTCATTAATA AACCTCAGGA AGAGCAATTT CTCAAAATCT CTACCAATTC AGTGGTCACT
    GGCTGTTTGC AGTTAAATGT CTGCAACTCC AACCCCTGTT TGCATGGAGG AAACTGTGAA
    GACATCTATA GCTCTTATCA TTGCTCCTGT CCCTTGGGAT GGTCAGGGAA ACACTGTGAA
    CTCAACATCG ATGAATGCTT TTCAAACCCC TGTATCCATG GCAACTGCTC TGACAGAGTT
    GCAGCCTACC ACTGCACATG TGAGCCTGGA TACACTGGTG TGAACTGTGA AGTGGATATA
    GACAACTGCC AGAGTCACCA GTGTGCAAAT GGAGCCACCT GCATTAGTCA TACTAATGGC
    TATTCTTGCC TCTGTTTTGG AAATTTTACA GGAAAATTTT GCAGACAGAG CAGATTACCC
    TCAACAGTCT GTGGGAATGA GAAGACAAAT CTCACTTGCT ACAATGGAGG CAACTGCACA
    GAGTTCCAGA CTGAATTAAA ATGTATGTGC CGGCCAGGTT TTACTGGAGA ATGGTGTGAA
    AAGGACATTG ATGAGTGTGC CTCTGATCCG TGTGTCAATG GAGGTCTGTG CCAGGACTTA
    CTCAACAAAT TCCAGTGCCT CTGTGATGTT GCCTTTGCTG GCGAGCGCTG CGAGGTGGAC
    TTGGCAGATG ACTTGATCTC CGACATTTTC ACCACTATTG GCTCAGTGAC TGTCGCCTTG
    TTACTGATCC TCTTGCTGGC CATTGTTGCT TCTGTTGTCA CCTCCAACAA AAGGGCAACT
    CAGGGAACCT ACAGCCCCAG CCGTCAGGAG AAGGAGGGCT CCCGAGTGGA AATGTGGAAC
    TTGATGCCAC CCCCTGCAAT GGAGAGACTG ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016856341.1
    CDS135..4490
    Translation

    Target ORF information:

    RefSeq Version XM_017000852.1
    Organism Homo sapiens(human)
    Definition Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017000852.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC
    TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG
    GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA
    CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA
    GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC
    TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT
    GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC
    AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG
    AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT
    TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA
    TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT
    TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT
    ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC
    CAAGATGGCC AGCATGGATT CAGCTGCCTA TGTCCATCTG GCTACACCGG GTCCCTGTGT
    GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA
    GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG
    GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC
    TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC
    AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT
    ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA
    AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG
    ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA
    GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT
    GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT
    TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG
    CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC
    TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC
    ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT
    CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC
    AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC
    AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT
    ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG
    ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA
    AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT
    GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGAGGC AGACCAATGT GGGAAGGGCA
    CTCACTGAGT TGGGATCCAG AGGACCTAAG TACCAAGTTT CACTGTTTCG CTTCTGTGTA
    GGATCTTGGG CAACTGGAAA CACCTTCTTT TTATCATCTA TAAAACCAGG ATCCAACCCC
    TGTCACAATG GAGGTGTTTG CCATTCCCGG TGGGATGACT TCTCCTGTTC CTGTCCTGCC
    CTCACAAGTG GGAAAGCCTG TGAGGAGGTT CAGTGGTGTG GATTCAGCCC GTGTCCTCAC
    GGAGCCCAGT GCCAGCCGGT GCTTCAAGGA TTTGAATGTA TTGCAAATGC TGTTTTTAAT
    GGACAAAGCG GTCAAATATT ATTCAGAAGC AATGGGAATA TTACCAGAGA ACTCACCAAT
    ATCACATTTG GTTTCAGAAC AAGGGATGCA AATGTAATAA TATTGCATGC AGAAAAAGAG
    CCTGAATTTC TTAATATTAG CATTCAAGAT TCCAGATTAT TCTTTCAATT GCAAAGTGGC
    AACAGCTTTT ATATGCTAAG TCTGACAAGT TTGCAGTCAG TGAATGATGG CACATGGCAC
    GAAGTGACCC TTTCCATGAC AGACCCACTG TCCCAGACCT CCAGGTGGCA AATGGAAGTG
    GACAACGAAA CACCTTTTGT GACCAGCACA ATTGCTACTG GAAGCCTCAA CTTTTTGAAG
    GATAATACAG ATATTTATGT GGGAGACAGA GCTATTGACA ATATAAAGGG CCTGCAAGGG
    TGTCTAAGTA CAATAGAAAT CGGAGGCATT TATCTCTCTT ACTTTGAAAA TGTTCATGGT
    TTCATTAATA AACCTCAGGA AGAGCAATTT CTCAAAATCT CTACCAATTC AGTGGTCACT
    GGCTGTTTGC AGTTAAATGT CTGCAACTCC AACCCCTGTT TGCATGGAGG AAACTGTGAA
    GACATCTATA GCTCTTATCA TTGCTCCTGT CCCTTGGGAT GGTCAGGGAA ACACTGTGAA
    CTCAACATCG ATGAATGCTT TTCAAACCCC TGTATCCATG GCAACTGCTC TGACAGAGTT
    GCAGCCTACC ACTGCACATG TGAGCCTGGA TACACTGGTG TGAACTGTGA AGTGGATATA
    GACAACTGCC AGAGTCACCA GTGTGCAAAT GGAGCCACCT GCATTAGTCA TACTAATGGC
    TATTCTTGCC TCTGTTTTGG AAATTTTACA GGAAAATTTT GCAGACAGAG CAGATTACCC
    TCAACAGTCT GTGGGAATGA GAAGACAAAT CTCACTTGCT ACAATGGAGG CAACTGCACA
    GAGTTCCAGA CTGAATTAAA ATGTATGTGC CGGCCAGGTT TTACTGGAGA ATGGTGTGAA
    AAGGACATTG ATGAGTGTGC CTCTGATCCG TGTGTCAATG GAGGTCTGTG CCAGGACTTA
    CTCAACAAAT TCCAGTGCCT CTGTGATGTT GCCTTTGCTG GCGAGCGCTG CGAGGTGGAC
    TTGGCAGATG ACTTGATCTC CGACATTTTC ACCACTATTG GCTCAGTGAC TGTCGCCTTG
    TTACTGATCC TCTTGCTGGC CATTGTTGCT TCTGTTGTCA CCTCCAACAA AAGGGCAACT
    CAGGGAACCT ACAGCCCCAG CCGTCAGGAG AAGGAGGGCT CCCGAGTGGA AATGTGGAAC
    TTGATGCCAC CCCCTGCAAT GGAGAGACTG ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17475
    Clone ID Related Accession (Same CDS sequence) NM_001193640.1
    Accession Version NM_001193640.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-21
    Organism Homo sapiens(human)
    Product protein crumbs homolog 1 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC370545.1, DC370777.1, AJ748821.1 and AY043325.1. Transcript Variant: This variant (2) lacks two in-frame exons compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.211656.1, SRR1803617.209649.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157437, SAMN03465402 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGATACACA
    GGTGCCCAGT GTGAGATCGA CCTCAATGAA TGCAATAGTA ACCCCTGCCA GTCCAATGGG
    GAATGTGTGG AGCTGTCCTC AGAGAAACAA TATGGACGCA TCACTGGACT GCCTTCTTCT
    TTCAGCTACC ATGAAGCCTC AGGTTATGTC TGTATCTGTC AGCCTGGATT CACAGGAATC
    CACTGCGAAG AAGACGTCAA TGAATGTTCT TCAAACCCTT GCCAAAATGG TGGTACTTGT
    GAGAACTTGC CTGGGAATTA TACTTGCCAT TGCCCATTTG ATAACCTTTC TAGAACTTTT
    TATGGAGGAA GGGACTGTTC TGATATTCTC CTGGGCTGTA CCCATCAGCA ATGTCTAAAT
    AATGGAACAT GCATCCCTCA CTTCCAAGAT GGCCAGCATG GATTCAGCTG CCTGTGTCCA
    TCTGGCTACA CCGGGTCCCT GTGTGAAATC GCAACCACAC TTTCATTTGA GGGCGATGGC
    TTCCTGTGGG TCAAAAGTGG CTCAGTGACA ACCAAGGGCT CAGTTTGTAA CATAGCCCTC
    AGGTTTCAGA CTGTTCAGCC AATGGCTCTT CTACTTTTCC GAAGCAACAG GGATGTGTTT
    GTGAAGCTGG AGCTGCTAAG TGGCTACATT CACTTATCAA TTCAGGTCAA TAATCAGTCA
    AAGGTGCTTC TGTTCATTTC CCACAACACC AGCGATGGAG AGTGGCATTT CGTGGAGGTA
    ATATTTGCAG AGGCTGTGAC CCTTACCTTA ATCGACGACT CCTGTAAGGA GAAATGCATC
    GCGAAAGCTC CTACTCCACT TGAAAGTGAT CAATCAATAT GTGCTTTTCA GAACTCCTTT
    TTGGGTGGTT TACCAGTGGG AATGACCAGC AATGGTGTTG CTCTGCTTAA CTTCTATAAT
    ATGCCATCCA CACCTTCGTT TGTAGGCTGT CTCCAAGACA TTAAAATTGA TTGGAATCAC
    ATTACCCTGG AGAACATCTC GTCTGGCTCA TCATTAAATG TCAAGGCAGG CTGTGTGAGA
    AAGGATTGGT GTGAAAGCCA ACCTTGTCAA AGCAGAGGAC GCTGCATCAA CTTGTGGCTG
    AGTTACCAGT GTGACTGCCA CAGGCCCTAT GAAGGCCCCA ACTGTCTGAG AGAGTATGTG
    GCAGGCAGAT TTGGCCAGGA TGACTCCACT GGTTATGTCA TCTTTACTCT TGATGAGAGC
    TATGGAGACA CCATCAGCCT CTCCATGTTT GTCCGAACGC TTCAACCATC AGGCTTACTT
    CTAGCTTTGG AAAACAGCAC TTATCAATAT ATCCGTGTCT GGCTAGAGCG CGGCAGACTA
    GCAATGCTGA CTCCAAACTC TCCCAAATTA GTAGTAAAAT TTGTTCTTAA TGATGGAAAT
    GTCCACTTGA TATCTTTGAA AATCAAGCCA TATAAAATTG AACTGTATCA GTCTTCACAA
    AACCTAGGAT TTATTTCTGC TTCTACGTGG AAAATCGAAA AGGGAGATGT CATCTACATT
    GGTGGCCTAC CTGACAAGCA AGAGACTGAA CTTAATGGTG GATTCTTCAA AGGCTGTATC
    CAAGATGTAA GACTAAACAA CCAAAATCTG GAATTCTTTC CAAATCCAAC AAACAATGCA
    TCTCTCAATC CAGTTCTTGT CAATGTAACC CAAGGCTGTG CTGGAGACAA CAGCTGCAAG
    TCCAACCCCT GTCACAATGG AGGTGTTTGC CATTCCCGGT GGGATGACTT CTCCTGTTCC
    TGTCCTGCCC TCACAAGTGG GAAAGCCTGT GAGGAGGTTC AGTGGTGTGG ATTCAGCCCG
    TGTCCTCACG GAGCCCAGTG CCAGCCGGTG CTTCAAGGAT TTGAATGTAT TGCAAATGCT
    GTTTTTAATG GACAAAGCGG TCAAATATTA TTCAGAAGCA ATGGGAATAT TACCAGAGAA
    CTCACCAATA TCACATTTGG TTTCAGAACA AGGGATGCAA ATGTAATAAT ATTGCATGCA
    GAAAAAGAGC CTGAATTTCT TAATATTAGC ATTCAAGATT CCAGATTATT CTTTCAATTG
    CAAAGTGGCA ACAGCTTTTA TATGCTAAGT CTGACAAGTT TGCAGTCAGT GAATGATGGC
    ACATGGCACG AAGTGACCCT TTCCATGACA GACCCACTGT CCCAGACCTC CAGGTGGCAA
    ATGGAAGTGG ACAACGAAAC ACCTTTTGTG ACCAGCACAA TTGCTACTGG AAGCCTCAAC
    TTTTTGAAGG ATAATACAGA TATTTATGTG GGAGACAGAG CTATTGACAA TATAAAGGGC
    CTGCAAGGGT GTCTAAGTAC AATAGAAATC GGAGGCATTT ATCTCTCTTA CTTTGAAAAT
    GTTCATGGTT TCATTAATAA ACCTCAGGAA GAGCAATTTC TCAAAATCTC TACCAATTCA
    GTGGTCACTG GCTGTTTGCA GTTAAATGTC TGCAACTCCA ACCCCTGTTT GCATGGAGGA
    AACTGTGAAG ACATCTATAG CTCTTATCAT TGCTCCTGTC CCTTGGGATG GTCAGGGAAA
    CACTGTGAAC TCAACATCGA TGAATGCTTT TCAAACCCCT GTATCCATGG CAACTGCTCT
    GACAGAGTTG CAGCCTACCA CTGCACATGT GAGCCTGGAT ACACTGGTGT GAACTGTGAA
    GTGGATATAG ACAACTGCCA GAGTCACCAG TGTGCAAATG GAGCCACCTG CATTAGTCAT
    ACTAATGGCT ATTCTTGCCT CTGTTTTGGA AATTTTACAG GAAAATTTTG CAGACAGAGC
    AGATTACCCT CAACAGTCTG TGGGAATGAG AAGACAAATC TCACTTGCTA CAATGGAGGC
    AACTGCACAG AGTTCCAGAC TGAATTAAAA TGTATGTGCC GGCCAGGTTT TACTGGAGAA
    TGGTGTGAAA AGGACATTGA TGAGTGTGCC TCTGATCCGT GTGTCAATGG AGGTCTGTGC
    CAGGACTTAC TCAACAAATT CCAGTGCCTC TGTGATGTTG CCTTTGCTGG CGAGCGCTGC
    GAGGTGGACT TGGCAGATGA CTTGATCTCC GACATTTTCA CCACTATTGG CTCAGTGACT
    GTCGCCTTGT TACTGATCCT CTTGCTGGCC ATTGTTGCTT CTGTTGTCAC CTCCAACAAA
    AGGGCAACTC AGGGAACCTA CAGCCCCAGC CGTCAGGAGA AGGAGGGCTC CCGAGTGGAA
    ATGTGGAACT TGATGCCACC CCCTGCAATG GAGAGACTGA TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001180569.1
    CDS210..4094
    Translation

    Target ORF information:

    RefSeq Version NM_001193640.1
    Organism Homo sapiens(human)
    Definition Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001193640.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGATACACA
    GGTGCCCAGT GTGAGATCGA CCTCAATGAA TGCAATAGTA ACCCCTGCCA GTCCAATGGG
    GAATGTGTGG AGCTGTCCTC AGAGAAACAA TATGGACGCA TCACTGGACT GCCTTCTTCT
    TTCAGCTACC ATGAAGCCTC AGGTTATGTC TGTATCTGTC AGCCTGGATT CACAGGAATC
    CACTGCGAAG AAGACGTCAA TGAATGTTCT TCAAACCCTT GCCAAAATGG TGGTACTTGT
    GAGAACTTGC CTGGGAATTA TACTTGCCAT TGCCCATTTG ATAACCTTTC TAGAACTTTT
    TATGGAGGAA GGGACTGTTC TGATATTCTC CTGGGCTGTA CCCATCAGCA ATGTCTAAAT
    AATGGAACAT GCATCCCTCA CTTCCAAGAT GGCCAGCATG GATTCAGCTG CCTGTGTCCA
    TCTGGCTACA CCGGGTCCCT GTGTGAAATC GCAACCACAC TTTCATTTGA GGGCGATGGC
    TTCCTGTGGG TCAAAAGTGG CTCAGTGACA ACCAAGGGCT CAGTTTGTAA CATAGCCCTC
    AGGTTTCAGA CTGTTCAGCC AATGGCTCTT CTACTTTTCC GAAGCAACAG GGATGTGTTT
    GTGAAGCTGG AGCTGCTAAG TGGCTACATT CACTTATCAA TTCAGGTCAA TAATCAGTCA
    AAGGTGCTTC TGTTCATTTC CCACAACACC AGCGATGGAG AGTGGCATTT CGTGGAGGTA
    ATATTTGCAG AGGCTGTGAC CCTTACCTTA ATCGACGACT CCTGTAAGGA GAAATGCATC
    GCGAAAGCTC CTACTCCACT TGAAAGTGAT CAATCAATAT GTGCTTTTCA GAACTCCTTT
    TTGGGTGGTT TACCAGTGGG AATGACCAGC AATGGTGTTG CTCTGCTTAA CTTCTATAAT
    ATGCCATCCA CACCTTCGTT TGTAGGCTGT CTCCAAGACA TTAAAATTGA TTGGAATCAC
    ATTACCCTGG AGAACATCTC GTCTGGCTCA TCATTAAATG TCAAGGCAGG CTGTGTGAGA
    AAGGATTGGT GTGAAAGCCA ACCTTGTCAA AGCAGAGGAC GCTGCATCAA CTTGTGGCTG
    AGTTACCAGT GTGACTGCCA CAGGCCCTAT GAAGGCCCCA ACTGTCTGAG AGAGTATGTG
    GCAGGCAGAT TTGGCCAGGA TGACTCCACT GGTTATGTCA TCTTTACTCT TGATGAGAGC
    TATGGAGACA CCATCAGCCT CTCCATGTTT GTCCGAACGC TTCAACCATC AGGCTTACTT
    CTAGCTTTGG AAAACAGCAC TTATCAATAT ATCCGTGTCT GGCTAGAGCG CGGCAGACTA
    GCAATGCTGA CTCCAAACTC TCCCAAATTA GTAGTAAAAT TTGTTCTTAA TGATGGAAAT
    GTCCACTTGA TATCTTTGAA AATCAAGCCA TATAAAATTG AACTGTATCA GTCTTCACAA
    AACCTAGGAT TTATTTCTGC TTCTACGTGG AAAATCGAAA AGGGAGATGT CATCTACATT
    GGTGGCCTAC CTGACAAGCA AGAGACTGAA CTTAATGGTG GATTCTTCAA AGGCTGTATC
    CAAGATGTAA GACTAAACAA CCAAAATCTG GAATTCTTTC CAAATCCAAC AAACAATGCA
    TCTCTCAATC CAGTTCTTGT CAATGTAACC CAAGGCTGTG CTGGAGACAA CAGCTGCAAG
    TCCAACCCCT GTCACAATGG AGGTGTTTGC CATTCCCGGT GGGATGACTT CTCCTGTTCC
    TGTCCTGCCC TCACAAGTGG GAAAGCCTGT GAGGAGGTTC AGTGGTGTGG ATTCAGCCCG
    TGTCCTCACG GAGCCCAGTG CCAGCCGGTG CTTCAAGGAT TTGAATGTAT TGCAAATGCT
    GTTTTTAATG GACAAAGCGG TCAAATATTA TTCAGAAGCA ATGGGAATAT TACCAGAGAA
    CTCACCAATA TCACATTTGG TTTCAGAACA AGGGATGCAA ATGTAATAAT ATTGCATGCA
    GAAAAAGAGC CTGAATTTCT TAATATTAGC ATTCAAGATT CCAGATTATT CTTTCAATTG
    CAAAGTGGCA ACAGCTTTTA TATGCTAAGT CTGACAAGTT TGCAGTCAGT GAATGATGGC
    ACATGGCACG AAGTGACCCT TTCCATGACA GACCCACTGT CCCAGACCTC CAGGTGGCAA
    ATGGAAGTGG ACAACGAAAC ACCTTTTGTG ACCAGCACAA TTGCTACTGG AAGCCTCAAC
    TTTTTGAAGG ATAATACAGA TATTTATGTG GGAGACAGAG CTATTGACAA TATAAAGGGC
    CTGCAAGGGT GTCTAAGTAC AATAGAAATC GGAGGCATTT ATCTCTCTTA CTTTGAAAAT
    GTTCATGGTT TCATTAATAA ACCTCAGGAA GAGCAATTTC TCAAAATCTC TACCAATTCA
    GTGGTCACTG GCTGTTTGCA GTTAAATGTC TGCAACTCCA ACCCCTGTTT GCATGGAGGA
    AACTGTGAAG ACATCTATAG CTCTTATCAT TGCTCCTGTC CCTTGGGATG GTCAGGGAAA
    CACTGTGAAC TCAACATCGA TGAATGCTTT TCAAACCCCT GTATCCATGG CAACTGCTCT
    GACAGAGTTG CAGCCTACCA CTGCACATGT GAGCCTGGAT ACACTGGTGT GAACTGTGAA
    GTGGATATAG ACAACTGCCA GAGTCACCAG TGTGCAAATG GAGCCACCTG CATTAGTCAT
    ACTAATGGCT ATTCTTGCCT CTGTTTTGGA AATTTTACAG GAAAATTTTG CAGACAGAGC
    AGATTACCCT CAACAGTCTG TGGGAATGAG AAGACAAATC TCACTTGCTA CAATGGAGGC
    AACTGCACAG AGTTCCAGAC TGAATTAAAA TGTATGTGCC GGCCAGGTTT TACTGGAGAA
    TGGTGTGAAA AGGACATTGA TGAGTGTGCC TCTGATCCGT GTGTCAATGG AGGTCTGTGC
    CAGGACTTAC TCAACAAATT CCAGTGCCTC TGTGATGTTG CCTTTGCTGG CGAGCGCTGC
    GAGGTGGACT TGGCAGATGA CTTGATCTCC GACATTTTCA CCACTATTGG CTCAGTGACT
    GTCGCCTTGT TACTGATCCT CTTGCTGGCC ATTGTTGCTT CTGTTGTCAC CTCCAACAAA
    AGGGCAACTC AGGGAACCTA CAGCCCCAGC CGTCAGGAGA AGGAGGGCTC CCGAGTGGAA
    ATGTGGAACT TGATGCCACC CCCTGCAATG GAGAGACTGA TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu66167
    Clone ID Related Accession (Same CDS sequence) XM_011509367.1
    Accession Version XM_011509367.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein crumbs homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC
    TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG
    GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA
    CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA
    GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC
    TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT
    GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC
    AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG
    AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT
    TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA
    TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT
    TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT
    ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC
    CAAGATGGCC AGCATGGATT CAGCTGCCTA TGTCCATCTG GCTACACCGG GTCCCTGTGT
    GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA
    GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG
    GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC
    TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC
    AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT
    ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA
    AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG
    ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA
    GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT
    GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT
    TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG
    CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC
    TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC
    ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT
    CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC
    AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC
    AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT
    ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG
    ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA
    AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT
    GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGTCCA ACCCCTGTCA CAATGGAGGT
    GTTTGCCATT CCCGGTGGGA TGACTTCTCC TGTTCCTGTC CTGCCCTCAC AAGTGGGAAA
    GCCTGTGAGG AGGTTCAGTG GTGTGGATTC AGCCCGTGTC CTCACGGAGC CCAGTGCCAG
    CCGGTGCTTC AAGGATTTGA ATGTATTGCA AATGCTGTTT TTAATGGACA AAGCGGTCAA
    ATATTATTCA GAAGCAATGG GAATATTACC AGAGAACTCA CCAATATCAC ATTTGGTTTC
    AGAACAAGGG ATGCAAATGT AATAATATTG CATGCAGAAA AAGAGCCTGA ATTTCTTAAT
    ATTAGCATTC AAGATTCCAG ATTATTCTTT CAATTGCAAA GTGGCAACAG CTTTTATATG
    CTAAGTCTGA CAAGTTTGCA GTCAGTGAAT GATGGCACAT GGCACGAAGT GACCCTTTCC
    ATGACAGACC CACTGTCCCA GACCTCCAGG TGGCAAATGG AAGTGGACAA CGAAACACCT
    TTTGTGACCA GCACAATTGC TACTGGAAGC CTCAACTTTT TGAAGGATAA TACAGATATT
    TATGTGGGAG ACAGAGCTAT TGACAATATA AAGGGCCTGC AAGGGTGTCT AAGTACAATA
    GAAATCGGAG GCATTTATCT CTCTTACTTT GAAAATGTTC ATGGTTTCAT TAATAAACCT
    CAGGAAGAGC AATTTCTCAA AATCTCTACC AATTCAGTGG TCACTGGCTG TTTGCAGTTA
    AATGTCTGCA ACTCCAACCC CTGTTTGCAT GGAGGAAACT GTGAAGACAT CTATAGCTCT
    TATCATTGCT CCTGTCCCTT GGGATGGTCA GGGAAACACT GTGAACTCAA CATCGATGAA
    TGCTTTTCAA ACCCCTGTAT CCATGGCAAC TGCTCTGACA GAGTTGCAGC CTACCACTGC
    ACATGTGAGC CTGGATACAC TGGTGTGAAC TGTGAAGTGG ATATAGACAA CTGCCAGAGT
    CACCAGTGTG CAAATGGAGC CACCTGCATT AGTCATACTA ATGGCTATTC TTGCCTCTGT
    TTTGGAAATT TTACAGGAAA ATTTTGCAGA CAGAGCAGAT TACCCTCAAC AGTCTGTGGG
    AATGAGAAGA CAAATCTCAC TTGCTACAAT GGAGGCAACT GCACAGAGTT CCAGACTGAA
    TTAAAATGTA TGTGCCGGCC AGGTTTTACT GGAGAATGTT GGCAGATGAC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011507669.1
    CDS135..4028
    Translation

    Target ORF information:

    RefSeq Version XM_011509367.1
    Organism Homo sapiens(human)
    Definition Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011509367.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC
    TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG
    GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA
    CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA
    GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC
    TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT
    GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC
    AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG
    AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT
    TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA
    TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT
    TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT
    ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC
    CAAGATGGCC AGCATGGATT CAGCTGCCTA TGTCCATCTG GCTACACCGG GTCCCTGTGT
    GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA
    GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG
    GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC
    TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC
    AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT
    ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA
    AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG
    ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA
    GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT
    GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT
    TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG
    CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC
    TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC
    ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT
    CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC
    AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC
    AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT
    ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG
    ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA
    AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT
    GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGTCCA ACCCCTGTCA CAATGGAGGT
    GTTTGCCATT CCCGGTGGGA TGACTTCTCC TGTTCCTGTC CTGCCCTCAC AAGTGGGAAA
    GCCTGTGAGG AGGTTCAGTG GTGTGGATTC AGCCCGTGTC CTCACGGAGC CCAGTGCCAG
    CCGGTGCTTC AAGGATTTGA ATGTATTGCA AATGCTGTTT TTAATGGACA AAGCGGTCAA
    ATATTATTCA GAAGCAATGG GAATATTACC AGAGAACTCA CCAATATCAC ATTTGGTTTC
    AGAACAAGGG ATGCAAATGT AATAATATTG CATGCAGAAA AAGAGCCTGA ATTTCTTAAT
    ATTAGCATTC AAGATTCCAG ATTATTCTTT CAATTGCAAA GTGGCAACAG CTTTTATATG
    CTAAGTCTGA CAAGTTTGCA GTCAGTGAAT GATGGCACAT GGCACGAAGT GACCCTTTCC
    ATGACAGACC CACTGTCCCA GACCTCCAGG TGGCAAATGG AAGTGGACAA CGAAACACCT
    TTTGTGACCA GCACAATTGC TACTGGAAGC CTCAACTTTT TGAAGGATAA TACAGATATT
    TATGTGGGAG ACAGAGCTAT TGACAATATA AAGGGCCTGC AAGGGTGTCT AAGTACAATA
    GAAATCGGAG GCATTTATCT CTCTTACTTT GAAAATGTTC ATGGTTTCAT TAATAAACCT
    CAGGAAGAGC AATTTCTCAA AATCTCTACC AATTCAGTGG TCACTGGCTG TTTGCAGTTA
    AATGTCTGCA ACTCCAACCC CTGTTTGCAT GGAGGAAACT GTGAAGACAT CTATAGCTCT
    TATCATTGCT CCTGTCCCTT GGGATGGTCA GGGAAACACT GTGAACTCAA CATCGATGAA
    TGCTTTTCAA ACCCCTGTAT CCATGGCAAC TGCTCTGACA GAGTTGCAGC CTACCACTGC
    ACATGTGAGC CTGGATACAC TGGTGTGAAC TGTGAAGTGG ATATAGACAA CTGCCAGAGT
    CACCAGTGTG CAAATGGAGC CACCTGCATT AGTCATACTA ATGGCTATTC TTGCCTCTGT
    TTTGGAAATT TTACAGGAAA ATTTTGCAGA CAGAGCAGAT TACCCTCAAC AGTCTGTGGG
    AATGAGAAGA CAAATCTCAC TTGCTACAAT GGAGGCAACT GCACAGAGTT CCAGACTGAA
    TTAAAATGTA TGTGCCGGCC AGGTTTTACT GGAGAATGTT GGCAGATGAC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22677
    Clone ID Related Accession (Same CDS sequence) NM_001257965.1
    Accession Version NM_001257965.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product protein crumbs homolog 1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302826.1, AK289733.1, AL136322.24 and AY043325.1. Transcript Variant: This variant (3) contains three noncoding exons in place of the first exon and contains an alternate in-frame exon compared to variant 1. The resulting isoform (3) is shorter at the N- terminus and contains an alternate internal segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302826.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968832, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAAGACC CTTGCTTCTC CAATCCCTGT CAAGGAAGTG CCACTTGTGT GAACACCCCA 
    GGAGAAAGGA GCTTTCTGTG CAAATGTCCT CCTGGGTACA GTGGGACAAT CTGTGAAACT
    ACCATTGGTT CCTGTGGCAA GAACTCCTGC CAACATGGAG GTATTTGCCA TCAGGACCCT
    ATTTATCCTG TCTGCATCTG CCCTGCTGGA TATGCTGGAA GATTCTGTGA GATAGATCAC
    GATGAGTGTG CTTCCAGCCC TTGCCAAAAT GGGGCCGTGT GCCAGGATGG AATTGATGGT
    TACTCCTGCT TCTGTGTCCC AGGATATCAA GGCAGACACT GCGACTTGGA AGTGGATGAA
    TGTGCTTCAG ATCCCTGCAA GAACGAGGCT ACATGCCTCA ATGAAATAGG AAGATATACT
    TGTATCTGTC CCCACAATTA TTCTGGTGTA AACTGTGAAT TGGAAATTGA CGAATGTTGG
    TCCCAGCCTT GTTTAAATGG TGCAACTTGT CAGGATGCTC TGGGGGCCTA TTTCTGCGAC
    TGTGCCCCTG GATTCCTGGG GGATCACTGT GAACTCAACA CTGATGAGTG TGCCAGTCAA
    CCTTGTCTCC ATGGAGGGCT GTGTGTGGAT GGAGAAAACA GATATAGCTG TAACTGCACG
    GGTAGTGGAT TCACAGGGAC ACACTGTGAG ACCTTGATGC CTCTTTGTTG GTCAAAACCT
    TGTCACAATA ATGCTACATG TGAGGACAGT GTTGACAATT ACACTTGTCA CTGCTGGCCT
    GGATACACAG GTGCCCAGTG TGAGATCGAC CTCAATGAAT GCAATAGTAA CCCCTGCCAG
    TCCAATGGGG AATGTGTGGA GCTGTCCTCA GAGAAACAAT ATGGACGCAT CACTGGACTG
    CCTTCTTCTT TCAGCTACCA TGAAGCCTCA GGTTATGTCT GTATCTGTCA GCCTGGATTC
    ACAGGAATCC ACTGCGAAGA AGACGTCAAT GAATGTTCTT CAAACCCTTG CCAAAATGGT
    GGTACTTGTG AGAACTTGCC TGGGAATTAT ACTTGCCATT GCCCATTTGA TAACCTTTCT
    AGAACTTTTT ATGGAGGAAG GGACTGTTCT GATATTCTCC TGGGCTGTAC CCATCAGCAA
    TGTCTAAATA ATGGAACATG CATCCCTCAC TTCCAAGATG GCCAGCATGG ATTCAGCTGC
    CTGTGTCCAT CTGGCTACAC CGGGTCCCTG TGTGAAATCG CAACCACACT TTCATTTGAG
    GGCGATGGCT TCCTGTGGGT CAAAAGTGGC TCAGTGACAA CCAAGGGCTC AGTTTGTAAC
    ATAGCCCTCA GGTTTCAGAC TGTTCAGCCA ATGGCTCTTC TACTTTTCCG AAGCAACAGG
    GATGTGTTTG TGAAGCTGGA GCTGCTAAGT GGCTACATTC ACTTATCAAT TCAGGTCAAT
    AATCAGTCAA AGGTGCTTCT GTTCATTTCC CACAACACCA GCGATGGAGA GTGGCATTTC
    GTGGAGGTAA TATTTGCAGA GGCTGTGACC CTTACCTTAA TCGACGACTC CTGTAAGGAG
    AAATGCATCG CGAAAGCTCC TACTCCACTT GAAAGTGATC AATCAATATG TGCTTTTCAG
    AACTCCTTTT TGGGTGGTTT ACCAGTGGGA ATGACCAGCA ATGGTGTTGC TCTGCTTAAC
    TTCTATAATA TGCCATCCAC ACCTTCGTTT GTAGGCTGTC TCCAAGACAT TAAAATTGAT
    TGGAATCACA TTACCCTGGA GAACATCTCG TCTGGCTCAT CATTAAATGT CAAGGCAGGC
    TGTGTGAGAA AGGATTGGTG TGAAAGCCAA CCTTGTCAAA GCAGAGGACG CTGCATCAAC
    TTGTGGCTGA GTTACCAGTG TGACTGCCAC AGGCCCTATG AAGGCCCCAA CTGTCTGAGA
    GAGTATGTGG CAGGCAGATT TGGCCAGGAT GACTCCACTG GTTATGTCAT CTTTACTCTT
    GATGAGAGCT ATGGAGACAC CATCAGCCTC TCCATGTTTG TCCGAACGCT TCAACCATCA
    GGCTTACTTC TAGCTTTGGA AAACAGCACT TATCAATATA TCCGTGTCTG GCTAGAGCGC
    GGCAGACTAG CAATGCTGAC TCCAAACTCT CCCAAATTAG TAGTAAAATT TGTTCTTAAT
    GATGGAAATG TCCACTTGAT ATCTTTGAAA ATCAAGCCAT ATAAAATTGA ACTGTATCAG
    TCTTCACAAA ACCTAGGATT TATTTCTGCT TCTACGTGGA AAATCGAAAA GGGAGATGTC
    ATCTACATTG GTGGCCTACC TGACAAGCAA GAGACTGAAC TTAATGGTGG ATTCTTCAAA
    GGCTGTATCC AAGATGTAAG ACTAAACAAC CAAAATCTGG AATTCTTTCC AAATCCAACA
    AACAATGCAT CTCTCAATCC AGTTCTTGTC AATGTAACCC AAGGCTGTGC TGGAGACAAC
    AGCTGCAAGA GGCAGACCAA TGTGGGAAGG GCACTCACTG AGTTGGGATC CAGAGGACCT
    AAGTACCAAG TTTCACTGTT TCGCTTCTGT GTAGGATCTT GGGCAACTGG AAACACCTTC
    TTTTTATCAT CTATAAAACC AGGATCCAAC CCCTGTCACA ATGGAGGTGT TTGCCATTCC
    CGGTGGGATG ACTTCTCCTG TTCCTGTCCT GCCCTCACAA GTGGGAAAGC CTGTGAGGAG
    GTTCAGTGGT GTGGATTCAG CCCGTGTCCT CACGGAGCCC AGTGCCAGCC GGTGCTTCAA
    GGATTTGAAT GTATTGCAAA TGCTGTTTTT AATGGACAAA GCGGTCAAAT ATTATTCAGA
    AGCAATGGGA ATATTACCAG AGAACTCACC AATATCACAT TTGGTTTCAG AACAAGGGAT
    GCAAATGTAA TAATATTGCA TGCAGAAAAA GAGCCTGAAT TTCTTAATAT TAGCATTCAA
    GATTCCAGAT TATTCTTTCA ATTGCAAAGT GGCAACAGCT TTTATATGCT AAGTCTGACA
    AGTTTGCAGT CAGTGAATGA TGGCACATGG CACGAAGTGA CCCTTTCCAT GACAGACCCA
    CTGTCCCAGA CCTCCAGGTG GCAAATGGAA GTGGACAACG AAACACCTTT TGTGACCAGC
    ACAATTGCTA CTGGAAGCCT CAACTTTTTG AAGGATAATA CAGATATTTA TGTGGGAGAC
    AGAGCTATTG ACAATATAAA GGGCCTGCAA GGGTGTCTAA GTACAATAGA AATCGGAGGC
    ATTTATCTCT CTTACTTTGA AAATGTTCAT GGTTTCATTA ATAAACCTCA GGAAGAGCAA
    TTTCTCAAAA TCTCTACCAA TTCAGTGGTC ACTGGCTGTT TGCAGTTAAA TGTCTGCAAC
    TCCAACCCCT GTTTGCATGG AGGAAACTGT GAAGACATCT ATAGCTCTTA TCATTGCTCC
    TGTCCCTTGG GATGGTCAGG GAAACACTGT GAACTCAACA TCGATGAATG CTTTTCAAAC
    CCCTGTATCC ATGGCAACTG CTCTGACAGA GTTGCAGCCT ACCACTGCAC ATGTGAGCCT
    GGATACACTG GTGTGAACTG TGAAGTGGAT ATAGACAACT GCCAGAGTCA CCAGTGTGCA
    AATGGAGCCA CCTGCATTAG TCATACTAAT GGCTATTCTT GCCTCTGTTT TGGAAATTTT
    ACAGGAAAAT TTTGCAGACA GAGCAGATTA CCCTCAACAG TCTGTGGGAA TGAGAAGACA
    AATCTCACTT GCTACAATGG AGGCAACTGC ACAGAGTTCC AGACTGAATT AAAATGTATG
    TGCCGGCCAG GTTTTACTGG AGAATGGTGT GAAAAGGACA TTGATGAGTG TGCCTCTGAT
    CCGTGTGTCA ATGGAGGTCT GTGCCAGGAC TTACTCAACA AATTCCAGTG CCTCTGTGAT
    GTTGCCTTTG CTGGCGAGCG CTGCGAGGTG GACTTGGCAG ATGACTTGAT CTCCGACATT
    TTCACCACTA TTGGCTCAGT GACTGTCGCC TTGTTACTGA TCCTCTTGCT GGCCATTGTT
    GCTTCTGTTG TCACCTCCAA CAAAAGGGCA ACTCAGGGAA CCTACAGCCC CAGCCGTCAG
    GAGAAGGAGG GCTCCCGAGT GGAAATGTGG AACTTGATGC CACCCCCTGC AATGGAGAGA
    CTGATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001244894.1
    CDS340..4488
    Translation

    Target ORF information:

    RefSeq Version NM_001257965.1
    Organism Homo sapiens(human)
    Definition Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001257965.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAAGACC CTTGCTTCTC CAATCCCTGT CAAGGAAGTG CCACTTGTGT GAACACCCCA 
    GGAGAAAGGA GCTTTCTGTG CAAATGTCCT CCTGGGTACA GTGGGACAAT CTGTGAAACT
    ACCATTGGTT CCTGTGGCAA GAACTCCTGC CAACATGGAG GTATTTGCCA TCAGGACCCT
    ATTTATCCTG TCTGCATCTG CCCTGCTGGA TATGCTGGAA GATTCTGTGA GATAGATCAC
    GATGAGTGTG CTTCCAGCCC TTGCCAAAAT GGGGCCGTGT GCCAGGATGG AATTGATGGT
    TACTCCTGCT TCTGTGTCCC AGGATATCAA GGCAGACACT GCGACTTGGA AGTGGATGAA
    TGTGCTTCAG ATCCCTGCAA GAACGAGGCT ACATGCCTCA ATGAAATAGG AAGATATACT
    TGTATCTGTC CCCACAATTA TTCTGGTGTA AACTGTGAAT TGGAAATTGA CGAATGTTGG
    TCCCAGCCTT GTTTAAATGG TGCAACTTGT CAGGATGCTC TGGGGGCCTA TTTCTGCGAC
    TGTGCCCCTG GATTCCTGGG GGATCACTGT GAACTCAACA CTGATGAGTG TGCCAGTCAA
    CCTTGTCTCC ATGGAGGGCT GTGTGTGGAT GGAGAAAACA GATATAGCTG TAACTGCACG
    GGTAGTGGAT TCACAGGGAC ACACTGTGAG ACCTTGATGC CTCTTTGTTG GTCAAAACCT
    TGTCACAATA ATGCTACATG TGAGGACAGT GTTGACAATT ACACTTGTCA CTGCTGGCCT
    GGATACACAG GTGCCCAGTG TGAGATCGAC CTCAATGAAT GCAATAGTAA CCCCTGCCAG
    TCCAATGGGG AATGTGTGGA GCTGTCCTCA GAGAAACAAT ATGGACGCAT CACTGGACTG
    CCTTCTTCTT TCAGCTACCA TGAAGCCTCA GGTTATGTCT GTATCTGTCA GCCTGGATTC
    ACAGGAATCC ACTGCGAAGA AGACGTCAAT GAATGTTCTT CAAACCCTTG CCAAAATGGT
    GGTACTTGTG AGAACTTGCC TGGGAATTAT ACTTGCCATT GCCCATTTGA TAACCTTTCT
    AGAACTTTTT ATGGAGGAAG GGACTGTTCT GATATTCTCC TGGGCTGTAC CCATCAGCAA
    TGTCTAAATA ATGGAACATG CATCCCTCAC TTCCAAGATG GCCAGCATGG ATTCAGCTGC
    CTGTGTCCAT CTGGCTACAC CGGGTCCCTG TGTGAAATCG CAACCACACT TTCATTTGAG
    GGCGATGGCT TCCTGTGGGT CAAAAGTGGC TCAGTGACAA CCAAGGGCTC AGTTTGTAAC
    ATAGCCCTCA GGTTTCAGAC TGTTCAGCCA ATGGCTCTTC TACTTTTCCG AAGCAACAGG
    GATGTGTTTG TGAAGCTGGA GCTGCTAAGT GGCTACATTC ACTTATCAAT TCAGGTCAAT
    AATCAGTCAA AGGTGCTTCT GTTCATTTCC CACAACACCA GCGATGGAGA GTGGCATTTC
    GTGGAGGTAA TATTTGCAGA GGCTGTGACC CTTACCTTAA TCGACGACTC CTGTAAGGAG
    AAATGCATCG CGAAAGCTCC TACTCCACTT GAAAGTGATC AATCAATATG TGCTTTTCAG
    AACTCCTTTT TGGGTGGTTT ACCAGTGGGA ATGACCAGCA ATGGTGTTGC TCTGCTTAAC
    TTCTATAATA TGCCATCCAC ACCTTCGTTT GTAGGCTGTC TCCAAGACAT TAAAATTGAT
    TGGAATCACA TTACCCTGGA GAACATCTCG TCTGGCTCAT CATTAAATGT CAAGGCAGGC
    TGTGTGAGAA AGGATTGGTG TGAAAGCCAA CCTTGTCAAA GCAGAGGACG CTGCATCAAC
    TTGTGGCTGA GTTACCAGTG TGACTGCCAC AGGCCCTATG AAGGCCCCAA CTGTCTGAGA
    GAGTATGTGG CAGGCAGATT TGGCCAGGAT GACTCCACTG GTTATGTCAT CTTTACTCTT
    GATGAGAGCT ATGGAGACAC CATCAGCCTC TCCATGTTTG TCCGAACGCT TCAACCATCA
    GGCTTACTTC TAGCTTTGGA AAACAGCACT TATCAATATA TCCGTGTCTG GCTAGAGCGC
    GGCAGACTAG CAATGCTGAC TCCAAACTCT CCCAAATTAG TAGTAAAATT TGTTCTTAAT
    GATGGAAATG TCCACTTGAT ATCTTTGAAA ATCAAGCCAT ATAAAATTGA ACTGTATCAG
    TCTTCACAAA ACCTAGGATT TATTTCTGCT TCTACGTGGA AAATCGAAAA GGGAGATGTC
    ATCTACATTG GTGGCCTACC TGACAAGCAA GAGACTGAAC TTAATGGTGG ATTCTTCAAA
    GGCTGTATCC AAGATGTAAG ACTAAACAAC CAAAATCTGG AATTCTTTCC AAATCCAACA
    AACAATGCAT CTCTCAATCC AGTTCTTGTC AATGTAACCC AAGGCTGTGC TGGAGACAAC
    AGCTGCAAGA GGCAGACCAA TGTGGGAAGG GCACTCACTG AGTTGGGATC CAGAGGACCT
    AAGTACCAAG TTTCACTGTT TCGCTTCTGT GTAGGATCTT GGGCAACTGG AAACACCTTC
    TTTTTATCAT CTATAAAACC AGGATCCAAC CCCTGTCACA ATGGAGGTGT TTGCCATTCC
    CGGTGGGATG ACTTCTCCTG TTCCTGTCCT GCCCTCACAA GTGGGAAAGC CTGTGAGGAG
    GTTCAGTGGT GTGGATTCAG CCCGTGTCCT CACGGAGCCC AGTGCCAGCC GGTGCTTCAA
    GGATTTGAAT GTATTGCAAA TGCTGTTTTT AATGGACAAA GCGGTCAAAT ATTATTCAGA
    AGCAATGGGA ATATTACCAG AGAACTCACC AATATCACAT TTGGTTTCAG AACAAGGGAT
    GCAAATGTAA TAATATTGCA TGCAGAAAAA GAGCCTGAAT TTCTTAATAT TAGCATTCAA
    GATTCCAGAT TATTCTTTCA ATTGCAAAGT GGCAACAGCT TTTATATGCT AAGTCTGACA
    AGTTTGCAGT CAGTGAATGA TGGCACATGG CACGAAGTGA CCCTTTCCAT GACAGACCCA
    CTGTCCCAGA CCTCCAGGTG GCAAATGGAA GTGGACAACG AAACACCTTT TGTGACCAGC
    ACAATTGCTA CTGGAAGCCT CAACTTTTTG AAGGATAATA CAGATATTTA TGTGGGAGAC
    AGAGCTATTG ACAATATAAA GGGCCTGCAA GGGTGTCTAA GTACAATAGA AATCGGAGGC
    ATTTATCTCT CTTACTTTGA AAATGTTCAT GGTTTCATTA ATAAACCTCA GGAAGAGCAA
    TTTCTCAAAA TCTCTACCAA TTCAGTGGTC ACTGGCTGTT TGCAGTTAAA TGTCTGCAAC
    TCCAACCCCT GTTTGCATGG AGGAAACTGT GAAGACATCT ATAGCTCTTA TCATTGCTCC
    TGTCCCTTGG GATGGTCAGG GAAACACTGT GAACTCAACA TCGATGAATG CTTTTCAAAC
    CCCTGTATCC ATGGCAACTG CTCTGACAGA GTTGCAGCCT ACCACTGCAC ATGTGAGCCT
    GGATACACTG GTGTGAACTG TGAAGTGGAT ATAGACAACT GCCAGAGTCA CCAGTGTGCA
    AATGGAGCCA CCTGCATTAG TCATACTAAT GGCTATTCTT GCCTCTGTTT TGGAAATTTT
    ACAGGAAAAT TTTGCAGACA GAGCAGATTA CCCTCAACAG TCTGTGGGAA TGAGAAGACA
    AATCTCACTT GCTACAATGG AGGCAACTGC ACAGAGTTCC AGACTGAATT AAAATGTATG
    TGCCGGCCAG GTTTTACTGG AGAATGGTGT GAAAAGGACA TTGATGAGTG TGCCTCTGAT
    CCGTGTGTCA ATGGAGGTCT GTGCCAGGAC TTACTCAACA AATTCCAGTG CCTCTGTGAT
    GTTGCCTTTG CTGGCGAGCG CTGCGAGGTG GACTTGGCAG ATGACTTGAT CTCCGACATT
    TTCACCACTA TTGGCTCAGT GACTGTCGCC TTGTTACTGA TCCTCTTGCT GGCCATTGTT
    GCTTCTGTTG TCACCTCCAA CAAAAGGGCA ACTCAGGGAA CCTACAGCCC CAGCCGTCAG
    GAGAAGGAGG GCTCCCGAGT GGAAATGTGG AACTTGATGC CACCCCCTGC AATGGAGAGA
    CTGATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu66165
    Clone ID Related Accession (Same CDS sequence) XM_011509365.2
    Accession Version XM_011509365.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4050bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein crumbs homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011509365.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC
    TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG
    GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA
    CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA
    GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC
    TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT
    GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC
    AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG
    AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT
    TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA
    TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT
    TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT
    ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC
    CAAGATGGCC AGCATGGATT CAGCTGCCTA TGTCCATCTG GCTACACCGG GTCCCTGTGT
    GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA
    GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG
    GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC
    TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC
    AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT
    ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA
    AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG
    ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA
    GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT
    GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT
    TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG
    CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC
    TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC
    ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT
    CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC
    AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC
    AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT
    ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG
    ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA
    AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT
    GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGTCCA ACCCCTGTCA CAATGGAGGT
    GTTTGCCATT CCCGGTGGGA TGACTTCTCC TGTTCCTGTC CTGCCCTCAC AAGTGGGAAA
    GCCTGTGAGG AGGTTCAGTG GTGTGGATTC AGCCCGTGTC CTCACGGAGC CCAGTGCCAG
    CCGGTGCTTC AAGGATTTGA ATGTATTGCA AATGCTGTTT TTAATGGACA AAGCGGTCAA
    ATATTATTCA GAAGCAATGG GAATATTACC AGAGAACTCA CCAATATCAC ATTTGGTTTC
    AGAACAAGGG ATGCAAATGT AATAATATTG CATGCAGAAA AAGAGCCTGA ATTTCTTAAT
    ATTAGCATTC AAGATTCCAG ATTATTCTTT CAATTGCAAA GTGGCAACAG CTTTTATATG
    CTAAGTCTGA CAAGTTTGCA GTCAGTGAAT GATGGCACAT GGCACGAAGT GACCCTTTCC
    ATGACAGACC CACTGTCCCA GACCTCCAGG TGGCAAATGG AAGTGGACAA CGAAACACCT
    TTTGTGACCA GCACAATTGC TACTGGAAGC CTCAACTTTT TGAAGGATAA TACAGATATT
    TATGTGGGAG ACAGAGCTAT TGACAATATA AAGGGCCTGC AAGGGTGTCT AAGTACAATA
    GAAATCGGAG GCATTTATCT CTCTTACTTT GAAAATGTTC ATGGTTTCAT TAATAAACCT
    CAGGAAGAGC AATTTCTCAA AATCTCTACC AATTCAGTGG TCACTGGCTG TTTGCAGTTA
    AATGTCTGCA ACTCCAACCC CTGTTTGCAT GGAGGAAACT GTGAAGACAT CTATAGCTCT
    TATCATTGCT CCTGTCCCTT GGGATGGTCA GGGAAACACT GTGAACTCAA CATCGATGAA
    TGCTTTTCAA ACCCCTGTAT CCATGGCAAC TGCTCTGACA GAGTTGCAGC CTACCACTGC
    ACATGTGAGC CTGGATACAC TGGTGTGAAC TGTGAAGTGG ATATAGACAA CTGCCAGAGT
    CACCAGTGTG CAAATGGAGC CACCTGCATT AGTCATACTA ATGGCTATTC TTGCCTCTGT
    TTTGGAAATT TTACAGGAAA ATTTTGCAGA CAGAGCAGAT TACCCTCAAC AGTCTGTGGG
    AATGAGAAGA CAAATCTCAC TTGCTACAAT GGAGGCAACT GCACAGAGTT CCAGACTGAA
    TTAAAATGTA TGTGCCGGCC AGGTTTTACT GGAGAATGGT GTGAAAAGGA CATTGATGAG
    TGTGCCTCTG ATCCGTGTGT CAATGGAGGT CTGTGCCAGG ACTTACTCAA CAAATTCCAG
    TGCCTCTGTG ATGTTGCCTT TGCTGGCGAG CGCTGCGAGG TGGACATCTC TGGGGAAGAA
    AAAGGAAATA AAAACCATCT CAATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011507667.1
    CDS135..4184
    Translation

    Target ORF information:

    RefSeq Version XM_011509365.2
    Organism Homo sapiens(human)
    Definition Homo sapiens crumbs cell polarity complex component 1 (CRB1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011509365.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
    TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
    AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
    AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
    GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
    GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
    CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
    GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
    GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
    AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
    TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC
    TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG
    GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA
    CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA
    GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC
    TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT
    GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC
    AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG
    AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT
    TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA
    TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT
    TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT
    ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC
    CAAGATGGCC AGCATGGATT CAGCTGCCTA TGTCCATCTG GCTACACCGG GTCCCTGTGT
    GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA
    GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG
    GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC
    TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC
    AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT
    ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA
    AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG
    ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA
    GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT
    GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT
    TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG
    CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC
    TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC
    ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT
    CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC
    AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC
    AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT
    ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG
    ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA
    AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT
    GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGTCCA ACCCCTGTCA CAATGGAGGT
    GTTTGCCATT CCCGGTGGGA TGACTTCTCC TGTTCCTGTC CTGCCCTCAC AAGTGGGAAA
    GCCTGTGAGG AGGTTCAGTG GTGTGGATTC AGCCCGTGTC CTCACGGAGC CCAGTGCCAG
    CCGGTGCTTC AAGGATTTGA ATGTATTGCA AATGCTGTTT TTAATGGACA AAGCGGTCAA
    ATATTATTCA GAAGCAATGG GAATATTACC AGAGAACTCA CCAATATCAC ATTTGGTTTC
    AGAACAAGGG ATGCAAATGT AATAATATTG CATGCAGAAA AAGAGCCTGA ATTTCTTAAT
    ATTAGCATTC AAGATTCCAG ATTATTCTTT CAATTGCAAA GTGGCAACAG CTTTTATATG
    CTAAGTCTGA CAAGTTTGCA GTCAGTGAAT GATGGCACAT GGCACGAAGT GACCCTTTCC
    ATGACAGACC CACTGTCCCA GACCTCCAGG TGGCAAATGG AAGTGGACAA CGAAACACCT
    TTTGTGACCA GCACAATTGC TACTGGAAGC CTCAACTTTT TGAAGGATAA TACAGATATT
    TATGTGGGAG ACAGAGCTAT TGACAATATA AAGGGCCTGC AAGGGTGTCT AAGTACAATA
    GAAATCGGAG GCATTTATCT CTCTTACTTT GAAAATGTTC ATGGTTTCAT TAATAAACCT
    CAGGAAGAGC AATTTCTCAA AATCTCTACC AATTCAGTGG TCACTGGCTG TTTGCAGTTA
    AATGTCTGCA ACTCCAACCC CTGTTTGCAT GGAGGAAACT GTGAAGACAT CTATAGCTCT
    TATCATTGCT CCTGTCCCTT GGGATGGTCA GGGAAACACT GTGAACTCAA CATCGATGAA
    TGCTTTTCAA ACCCCTGTAT CCATGGCAAC TGCTCTGACA GAGTTGCAGC CTACCACTGC
    ACATGTGAGC CTGGATACAC TGGTGTGAAC TGTGAAGTGG ATATAGACAA CTGCCAGAGT
    CACCAGTGTG CAAATGGAGC CACCTGCATT AGTCATACTA ATGGCTATTC TTGCCTCTGT
    TTTGGAAATT TTACAGGAAA ATTTTGCAGA CAGAGCAGAT TACCCTCAAC AGTCTGTGGG
    AATGAGAAGA CAAATCTCAC TTGCTACAAT GGAGGCAACT GCACAGAGTT CCAGACTGAA
    TTAAAATGTA TGTGCCGGCC AGGTTTTACT GGAGAATGGT GTGAAAAGGA CATTGATGAG
    TGTGCCTCTG ATCCGTGTGT CAATGGAGGT CTGTGCCAGG ACTTACTCAA CAAATTCCAG
    TGCCTCTGTG ATGTTGCCTT TGCTGGCGAG CGCTGCGAGG TGGACATCTC TGGGGAAGAA
    AAAGGAAATA AAAACCATCT CAATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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