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CRB1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol CRB1
Entrez Gene ID 23418
Full Name crumbs family member 1, photoreceptor morphogenesis associated
Synonyms LCA8, RP12
General protein information
Preferred Names
protein crumbs homolog 1
Names
protein crumbs homolog 1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1q31.3

Summary This gene encodes a protein which is similar to the Drosophila crumbs protein and localizes to the inner segment of mammalian photoreceptors. In Drosophila crumbs localizes to the stalk of the fly photoreceptor and may be a component of the molecular scaffold that controls proper development of polarity in the eye. Mutations in this gene are associated with a severe form of retinitis pigmentosa, RP12, and with Leber congenital amaurosis. Alternate splicing results in multiple transcript variants, some protein coding and some non-protein coding.[provided by RefSeq, Apr 2012]. lac of sum
Disorder MIM:

604210

Disorder Html: Retinitis pigmentosa-12, autosomal recessive, 600105 (3);

mRNA and Protein(s)

mRNA Protein Name
XM_011509365 XP_011507667 protein crumbs homolog 1 isoform X1
XM_011509366 XP_011507668 protein crumbs homolog 1 isoform X2
XM_011509367 XP_011507669 protein crumbs homolog 1 isoform X3
XM_011509368 XP_011507670 protein crumbs homolog 1 isoform X4
XM_011509369 XP_011507671 protein crumbs homolog 1 isoform X5
NM_201253 NP_957705 protein crumbs homolog 1 isoform 1 precursor
NM_001193640 NP_001180569 protein crumbs homolog 1 isoform 2 precursor
NM_001257965 NP_001244894 protein crumbs homolog 1 isoform 3
NM_001257966 NP_001244895 protein crumbs homolog 1 isoform 4 precursor



Homo sapiens (human) CRB1 NP_957705.1
Pan troglodytes (chimpanzee) CRB1 XP_525009.2
Macaca mulatta (Rhesus monkey) CRB1 XP_001110912.1
Canis lupus familiaris (dog) CRB1 XP_005622350.1
Bos taurus (cattle) CRB1 NP_001179411.1
Mus musculus (house mouse) Crb1 NP_573502.2
Rattus norvegicus (Norway rat) Crb1 NP_001100652.1
Gallus gallus (chicken) CRB1 XP_003641718.2
Danio rerio (zebrafish) crb1 NP_001038408.1
Xenopus (Silurana) tropicalis (western clawed frog) crb1 XP_002932223.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CRB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu66165 XM_011509365 PREDICTED: Homo sapiens crumbs family member 1, photoreceptor morphogenesis associated (CRB1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu66166 XM_011509366 PREDICTED: Homo sapiens crumbs family member 1, photoreceptor morphogenesis associated (CRB1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu66167 XM_011509367 PREDICTED: Homo sapiens crumbs family member 1, photoreceptor morphogenesis associated (CRB1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu66168 XM_011509368 PREDICTED: Homo sapiens crumbs family member 1, photoreceptor morphogenesis associated (CRB1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu66169 XM_011509369 PREDICTED: Homo sapiens crumbs family member 1, photoreceptor morphogenesis associated (CRB1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $475.30
$679.00
OHu19399 NM_201253 Homo sapiens crumbs family member 1, photoreceptor morphogenesis associated (CRB1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 16 $727.30
$1039.00
OHu17475 NM_001193640 Homo sapiens crumbs family member 1, photoreceptor morphogenesis associated (CRB1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu22677 NM_001257965 Homo sapiens crumbs family member 1, photoreceptor morphogenesis associated (CRB1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu22808 NM_001257966 Homo sapiens crumbs family member 1, photoreceptor morphogenesis associated (CRB1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu66165
Clone ID Related Accession (Same CDS sequence) XM_011509365
Accession Version XM_011509365.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4050bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product protein crumbs homolog 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC
TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG
GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA
CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA
GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC
TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT
GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC
AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG
AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT
TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA
TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT
TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT
ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC
CAAGATGGCC AGCATGGATT CAGCTGCCTA TGTCCATCTG GCTACACCGG GTCCCTGTGT
GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA
GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG
GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC
TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC
AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT
ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA
AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG
ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA
GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT
GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT
TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG
CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC
TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC
ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT
CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC
AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC
AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT
ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG
ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA
AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT
GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGTCCA ACCCCTGTCA CAATGGAGGT
GTTTGCCATT CCCGGTGGGA TGACTTCTCC TGTTCCTGTC CTGCCCTCAC AAGTGGGAAA
GCCTGTGAGG AGGTTCAGTG GTGTGGATTC AGCCCGTGTC CTCACGGAGC CCAGTGCCAG
CCGGTGCTTC AAGGATTTGA ATGTATTGCA AATGCTGTTT TTAATGGACA AAGCGGTCAA
ATATTATTCA GAAGCAATGG GAATATTACC AGAGAACTCA CCAATATCAC ATTTGGTTTC
AGAACAAGGG ATGCAAATGT AATAATATTG CATGCAGAAA AAGAGCCTGA ATTTCTTAAT
ATTAGCATTC AAGATTCCAG ATTATTCTTT CAATTGCAAA GTGGCAACAG CTTTTATATG
CTAAGTCTGA CAAGTTTGCA GTCAGTGAAT GATGGCACAT GGCACGAAGT GACCCTTTCC
ATGACAGACC CACTGTCCCA GACCTCCAGG TGGCAAATGG AAGTGGACAA CGAAACACCT
TTTGTGACCA GCACAATTGC TACTGGAAGC CTCAACTTTT TGAAGGATAA TACAGATATT
TATGTGGGAG ACAGAGCTAT TGACAATATA AAGGGCCTGC AAGGGTGTCT AAGTACAATA
GAAATCGGAG GCATTTATCT CTCTTACTTT GAAAATGTTC ATGGTTTCAT TAATAAACCT
CAGGAAGAGC AATTTCTCAA AATCTCTACC AATTCAGTGG TCACTGGCTG TTTGCAGTTA
AATGTCTGCA ACTCCAACCC CTGTTTGCAT GGAGGAAACT GTGAAGACAT CTATAGCTCT
TATCATTGCT CCTGTCCCTT GGGATGGTCA GGGAAACACT GTGAACTCAA CATCGATGAA
TGCTTTTCAA ACCCCTGTAT CCATGGCAAC TGCTCTGACA GAGTTGCAGC CTACCACTGC
ACATGTGAGC CTGGATACAC TGGTGTGAAC TGTGAAGTGG ATATAGACAA CTGCCAGAGT
CACCAGTGTG CAAATGGAGC CACCTGCATT AGTCATACTA ATGGCTATTC TTGCCTCTGT
TTTGGAAATT TTACAGGAAA ATTTTGCAGA CAGAGCAGAT TACCCTCAAC AGTCTGTGGG
AATGAGAAGA CAAATCTCAC TTGCTACAAT GGAGGCAACT GCACAGAGTT CCAGACTGAA
TTAAAATGTA TGTGCCGGCC AGGTTTTACT GGAGAATGGT GTGAAAAGGA CATTGATGAG
TGTGCCTCTG ATCCGTGTGT CAATGGAGGT CTGTGCCAGG ACTTACTCAA CAAATTCCAG
TGCCTCTGTG ATGTTGCCTT TGCTGGCGAG CGCTGCGAGG TGGACATCTC TGGGGAAGAA
AAAGGAAATA AAAACCATCT CAATAATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011507667.1
CDS135..4184
Misc Feature(1)348..458(+)
Misc Feature(2)351..395(+)
Misc Feature(3)576..683(+)
Misc Feature(4)576..629(+)
Misc Feature(5)693..800(+)
Misc Feature(6)699..743(+)
Misc Feature(7)807..914(+)
Misc Feature(8)813..857(+)
Misc Feature(9)918..1031(+)
Misc Feature(10)918..971(+)
Misc Feature(11)1059..1145(+)
Misc Feature(12)1323..>1406(+)
Misc Feature(13)1323..1376(+)
Misc Feature(14)1587..2081(+)
Misc Feature(15)2160..2255(+)
Misc Feature(16)2292..2708(+)
Misc Feature(17)2799..2903(+)
Misc Feature(18)2802..2846(+)
Misc Feature(19)2988..3440(+)
Misc Feature(20)3570..3659(+)
Misc Feature(21)3663..3770(+)
Misc Feature(22)3663..3713(+)
Misc Feature(23)3774..3881(+)
Misc Feature(24)3774..3827(+)
Misc Feature(25)4023..4133(+)
Misc Feature(26)4023..4076(+)
Translation

Target ORF information:

RefSeq Version XM_011509365
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens crumbs family member 1, photoreceptor morphogenesis associated (CRB1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509365

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC
TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG
GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA
CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA
GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC
TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT
GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC
AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG
AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT
TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA
TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT
TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT
ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC
CAAGATGGCC AGCATGGATT CAGCTGCCTA TGTCCATCTG GCTACACCGG GTCCCTGTGT
GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA
GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG
GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC
TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC
AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT
ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA
AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG
ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA
GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT
GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT
TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG
CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC
TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC
ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT
CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC
AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC
AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT
ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG
ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA
AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT
GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGTCCA ACCCCTGTCA CAATGGAGGT
GTTTGCCATT CCCGGTGGGA TGACTTCTCC TGTTCCTGTC CTGCCCTCAC AAGTGGGAAA
GCCTGTGAGG AGGTTCAGTG GTGTGGATTC AGCCCGTGTC CTCACGGAGC CCAGTGCCAG
CCGGTGCTTC AAGGATTTGA ATGTATTGCA AATGCTGTTT TTAATGGACA AAGCGGTCAA
ATATTATTCA GAAGCAATGG GAATATTACC AGAGAACTCA CCAATATCAC ATTTGGTTTC
AGAACAAGGG ATGCAAATGT AATAATATTG CATGCAGAAA AAGAGCCTGA ATTTCTTAAT
ATTAGCATTC AAGATTCCAG ATTATTCTTT CAATTGCAAA GTGGCAACAG CTTTTATATG
CTAAGTCTGA CAAGTTTGCA GTCAGTGAAT GATGGCACAT GGCACGAAGT GACCCTTTCC
ATGACAGACC CACTGTCCCA GACCTCCAGG TGGCAAATGG AAGTGGACAA CGAAACACCT
TTTGTGACCA GCACAATTGC TACTGGAAGC CTCAACTTTT TGAAGGATAA TACAGATATT
TATGTGGGAG ACAGAGCTAT TGACAATATA AAGGGCCTGC AAGGGTGTCT AAGTACAATA
GAAATCGGAG GCATTTATCT CTCTTACTTT GAAAATGTTC ATGGTTTCAT TAATAAACCT
CAGGAAGAGC AATTTCTCAA AATCTCTACC AATTCAGTGG TCACTGGCTG TTTGCAGTTA
AATGTCTGCA ACTCCAACCC CTGTTTGCAT GGAGGAAACT GTGAAGACAT CTATAGCTCT
TATCATTGCT CCTGTCCCTT GGGATGGTCA GGGAAACACT GTGAACTCAA CATCGATGAA
TGCTTTTCAA ACCCCTGTAT CCATGGCAAC TGCTCTGACA GAGTTGCAGC CTACCACTGC
ACATGTGAGC CTGGATACAC TGGTGTGAAC TGTGAAGTGG ATATAGACAA CTGCCAGAGT
CACCAGTGTG CAAATGGAGC CACCTGCATT AGTCATACTA ATGGCTATTC TTGCCTCTGT
TTTGGAAATT TTACAGGAAA ATTTTGCAGA CAGAGCAGAT TACCCTCAAC AGTCTGTGGG
AATGAGAAGA CAAATCTCAC TTGCTACAAT GGAGGCAACT GCACAGAGTT CCAGACTGAA
TTAAAATGTA TGTGCCGGCC AGGTTTTACT GGAGAATGGT GTGAAAAGGA CATTGATGAG
TGTGCCTCTG ATCCGTGTGT CAATGGAGGT CTGTGCCAGG ACTTACTCAA CAAATTCCAG
TGCCTCTGTG ATGTTGCCTT TGCTGGCGAG CGCTGCGAGG TGGACATCTC TGGGGAAGAA
AAAGGAAATA AAAACCATCT CAATAATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu66166
Clone ID Related Accession (Same CDS sequence) XM_011509366
Accession Version XM_011509366.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4011bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product protein crumbs homolog 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC
TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG
GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA
CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA
GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC
TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT
GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC
AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG
AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT
TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA
TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT
TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT
ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC
CAAGATGGCC AGCATGGATT CAGCTGCCTA TGTCCATCTG GCTACACCGG GTCCCTGTGT
GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA
GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG
GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC
TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC
AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT
ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA
AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG
ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA
GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT
GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT
TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG
CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC
TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC
ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT
CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC
AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC
AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT
ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG
ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA
AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT
GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGTCCA ACCCCTGTCA CAATGGAGGT
GTTTGCCATT CCCGGTGGGA TGACTTCTCC TGTTCCTGTC CTGCCCTCAC AAGTGGGAAA
GCCTGTGAGG AGGTTCAGTG GTGTGGATTC AGCCCGTGTC CTCACGGAGC CCAGTGCCAG
CCGGTGCTTC AAGGATTTGA ATGTATTGCA AATGCTGTTT TTAATGGACA AAGCGGTCAA
ATATTATTCA GAAGCAATGG GAATATTACC AGAGAACTCA CCAATATCAC ATTTGGTTTC
AGAACAAGGG ATGCAAATGT AATAATATTG CATGCAGAAA AAGAGCCTGA ATTTCTTAAT
ATTAGCATTC AAGATTCCAG ATTATTCTTT CAATTGCAAA GTGGCAACAG CTTTTATATG
CTAAGTCTGA CAAGTTTGCA GTCAGTGAAT GATGGCACAT GGCACGAAGT GACCCTTTCC
ATGACAGACC CACTGTCCCA GACCTCCAGG TGGCAAATGG AAGTGGACAA CGAAACACCT
TTTGTGACCA GCACAATTGC TACTGGAAGC CTCAACTTTT TGAAGGATAA TACAGATATT
TATGTGGGAG ACAGAGCTAT TGACAATATA AAGGGCCTGC AAGGGTGTCT AAGTACAATA
GAAATCGGAG GCATTTATCT CTCTTACTTT GAAAATGTTC ATGGTTTCAT TAATAAACCT
CAGGAAGAGC AATTTCTCAA AATCTCTACC AATTCAGTGG TCACTGGCTG TTTGCAGTTA
AATGTCTGCA ACTCCAACCC CTGTTTGCAT GGAGGAAACT GTGAAGACAT CTATAGCTCT
TATCATTGCT CCTGTCCCTT GGGATGGTCA GGGAAACACT GTGAACTCAA CATCGATGAA
TGCTTTTCAA ACCCCTGTAT CCATGGCAAC TGCTCTGACA GAGTTGCAGC CTACCACTGC
ACATGTGAGC CTGGATACAC TGGTGTGAAC TGTGAAGTGG ATATAGACAA CTGCCAGAGT
CACCAGTGTG CAAATGGAGC CACCTGCATT AGTCATACTA ATGGCTATTC TTGCCTCTGT
TTTGGAAATT TTACAGGAAA ATTTTGCAGA CAGAGCAGAT TACCCTCAAC AGTCTGTGGG
AATGAGAAGA CAAATCTCAC TTGCTACAAT GGAGGCAACT GCACAGAGTT CCAGACTGAA
TTAAAATGTA TGTGCCGGCC AGGTTTTACT GGAGAATGGT GTGAAAAGGA CATTGATGAG
TGTGCCTCTG ATCCGTGTGT CAATGGAGGT CTGTGCCAGG ACTTACTCAA CAAATTCCAG
TGCCTCTGTG ATGTTGCCTT TGCTGGCGAG CGCTGCGAGG TGGACTACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011507668.1
CDS135..4145
Misc Feature(1)348..458(+)
Misc Feature(2)351..395(+)
Misc Feature(3)576..683(+)
Misc Feature(4)576..629(+)
Misc Feature(5)693..800(+)
Misc Feature(6)699..743(+)
Misc Feature(7)807..914(+)
Misc Feature(8)813..857(+)
Misc Feature(9)918..1031(+)
Misc Feature(10)918..971(+)
Misc Feature(11)1059..1145(+)
Misc Feature(12)1323..>1406(+)
Misc Feature(13)1323..1376(+)
Misc Feature(14)1587..2081(+)
Misc Feature(15)2160..2255(+)
Misc Feature(16)2292..2708(+)
Misc Feature(17)2799..2903(+)
Misc Feature(18)2802..2846(+)
Misc Feature(19)2988..3440(+)
Misc Feature(20)3570..3659(+)
Misc Feature(21)3663..3770(+)
Misc Feature(22)3663..3713(+)
Misc Feature(23)3774..3881(+)
Misc Feature(24)3774..3827(+)
Misc Feature(25)4023..4133(+)
Misc Feature(26)4023..4076(+)
Translation

Target ORF information:

RefSeq Version XM_011509366
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens crumbs family member 1, photoreceptor morphogenesis associated (CRB1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509366

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC
TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG
GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA
CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA
GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC
TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT
GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC
AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG
AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT
TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA
TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT
TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT
ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC
CAAGATGGCC AGCATGGATT CAGCTGCCTA TGTCCATCTG GCTACACCGG GTCCCTGTGT
GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA
GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG
GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC
TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC
AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT
ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA
AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG
ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA
GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT
GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT
TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG
CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC
TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC
ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT
CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC
AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC
AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT
ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG
ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA
AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT
GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGTCCA ACCCCTGTCA CAATGGAGGT
GTTTGCCATT CCCGGTGGGA TGACTTCTCC TGTTCCTGTC CTGCCCTCAC AAGTGGGAAA
GCCTGTGAGG AGGTTCAGTG GTGTGGATTC AGCCCGTGTC CTCACGGAGC CCAGTGCCAG
CCGGTGCTTC AAGGATTTGA ATGTATTGCA AATGCTGTTT TTAATGGACA AAGCGGTCAA
ATATTATTCA GAAGCAATGG GAATATTACC AGAGAACTCA CCAATATCAC ATTTGGTTTC
AGAACAAGGG ATGCAAATGT AATAATATTG CATGCAGAAA AAGAGCCTGA ATTTCTTAAT
ATTAGCATTC AAGATTCCAG ATTATTCTTT CAATTGCAAA GTGGCAACAG CTTTTATATG
CTAAGTCTGA CAAGTTTGCA GTCAGTGAAT GATGGCACAT GGCACGAAGT GACCCTTTCC
ATGACAGACC CACTGTCCCA GACCTCCAGG TGGCAAATGG AAGTGGACAA CGAAACACCT
TTTGTGACCA GCACAATTGC TACTGGAAGC CTCAACTTTT TGAAGGATAA TACAGATATT
TATGTGGGAG ACAGAGCTAT TGACAATATA AAGGGCCTGC AAGGGTGTCT AAGTACAATA
GAAATCGGAG GCATTTATCT CTCTTACTTT GAAAATGTTC ATGGTTTCAT TAATAAACCT
CAGGAAGAGC AATTTCTCAA AATCTCTACC AATTCAGTGG TCACTGGCTG TTTGCAGTTA
AATGTCTGCA ACTCCAACCC CTGTTTGCAT GGAGGAAACT GTGAAGACAT CTATAGCTCT
TATCATTGCT CCTGTCCCTT GGGATGGTCA GGGAAACACT GTGAACTCAA CATCGATGAA
TGCTTTTCAA ACCCCTGTAT CCATGGCAAC TGCTCTGACA GAGTTGCAGC CTACCACTGC
ACATGTGAGC CTGGATACAC TGGTGTGAAC TGTGAAGTGG ATATAGACAA CTGCCAGAGT
CACCAGTGTG CAAATGGAGC CACCTGCATT AGTCATACTA ATGGCTATTC TTGCCTCTGT
TTTGGAAATT TTACAGGAAA ATTTTGCAGA CAGAGCAGAT TACCCTCAAC AGTCTGTGGG
AATGAGAAGA CAAATCTCAC TTGCTACAAT GGAGGCAACT GCACAGAGTT CCAGACTGAA
TTAAAATGTA TGTGCCGGCC AGGTTTTACT GGAGAATGGT GTGAAAAGGA CATTGATGAG
TGTGCCTCTG ATCCGTGTGT CAATGGAGGT CTGTGCCAGG ACTTACTCAA CAAATTCCAG
TGCCTCTGTG ATGTTGCCTT TGCTGGCGAG CGCTGCGAGG TGGACTACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu66167
Clone ID Related Accession (Same CDS sequence) XM_011509367
Accession Version XM_011509367.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3894bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product protein crumbs homolog 1 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC
TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG
GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA
CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA
GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC
TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT
GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC
AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG
AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT
TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA
TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT
TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT
ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC
CAAGATGGCC AGCATGGATT CAGCTGCCTA TGTCCATCTG GCTACACCGG GTCCCTGTGT
GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA
GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG
GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC
TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC
AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT
ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA
AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG
ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA
GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT
GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT
TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG
CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC
TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC
ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT
CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC
AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC
AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT
ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG
ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA
AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT
GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGTCCA ACCCCTGTCA CAATGGAGGT
GTTTGCCATT CCCGGTGGGA TGACTTCTCC TGTTCCTGTC CTGCCCTCAC AAGTGGGAAA
GCCTGTGAGG AGGTTCAGTG GTGTGGATTC AGCCCGTGTC CTCACGGAGC CCAGTGCCAG
CCGGTGCTTC AAGGATTTGA ATGTATTGCA AATGCTGTTT TTAATGGACA AAGCGGTCAA
ATATTATTCA GAAGCAATGG GAATATTACC AGAGAACTCA CCAATATCAC ATTTGGTTTC
AGAACAAGGG ATGCAAATGT AATAATATTG CATGCAGAAA AAGAGCCTGA ATTTCTTAAT
ATTAGCATTC AAGATTCCAG ATTATTCTTT CAATTGCAAA GTGGCAACAG CTTTTATATG
CTAAGTCTGA CAAGTTTGCA GTCAGTGAAT GATGGCACAT GGCACGAAGT GACCCTTTCC
ATGACAGACC CACTGTCCCA GACCTCCAGG TGGCAAATGG AAGTGGACAA CGAAACACCT
TTTGTGACCA GCACAATTGC TACTGGAAGC CTCAACTTTT TGAAGGATAA TACAGATATT
TATGTGGGAG ACAGAGCTAT TGACAATATA AAGGGCCTGC AAGGGTGTCT AAGTACAATA
GAAATCGGAG GCATTTATCT CTCTTACTTT GAAAATGTTC ATGGTTTCAT TAATAAACCT
CAGGAAGAGC AATTTCTCAA AATCTCTACC AATTCAGTGG TCACTGGCTG TTTGCAGTTA
AATGTCTGCA ACTCCAACCC CTGTTTGCAT GGAGGAAACT GTGAAGACAT CTATAGCTCT
TATCATTGCT CCTGTCCCTT GGGATGGTCA GGGAAACACT GTGAACTCAA CATCGATGAA
TGCTTTTCAA ACCCCTGTAT CCATGGCAAC TGCTCTGACA GAGTTGCAGC CTACCACTGC
ACATGTGAGC CTGGATACAC TGGTGTGAAC TGTGAAGTGG ATATAGACAA CTGCCAGAGT
CACCAGTGTG CAAATGGAGC CACCTGCATT AGTCATACTA ATGGCTATTC TTGCCTCTGT
TTTGGAAATT TTACAGGAAA ATTTTGCAGA CAGAGCAGAT TACCCTCAAC AGTCTGTGGG
AATGAGAAGA CAAATCTCAC TTGCTACAAT GGAGGCAACT GCACAGAGTT CCAGACTGAA
TTAAAATGTA TGTGCCGGCC AGGTTTTACT GGAGAATGTT GGCAGATGAC TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011507669.1
CDS135..4028
Misc Feature(1)348..458(+)
Misc Feature(2)351..395(+)
Misc Feature(3)576..683(+)
Misc Feature(4)576..629(+)
Misc Feature(5)693..800(+)
Misc Feature(6)699..743(+)
Misc Feature(7)807..914(+)
Misc Feature(8)813..857(+)
Misc Feature(9)918..1031(+)
Misc Feature(10)918..971(+)
Misc Feature(11)1059..1145(+)
Misc Feature(12)1323..>1406(+)
Misc Feature(13)1323..1376(+)
Misc Feature(14)1587..2081(+)
Misc Feature(15)2160..2255(+)
Misc Feature(16)2292..2708(+)
Misc Feature(17)2799..2903(+)
Misc Feature(18)2802..2846(+)
Misc Feature(19)2988..3440(+)
Misc Feature(20)3570..3659(+)
Misc Feature(21)3663..3770(+)
Misc Feature(22)3663..3713(+)
Misc Feature(23)3774..3881(+)
Misc Feature(24)3774..3827(+)
Translation

Target ORF information:

RefSeq Version XM_011509367
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens crumbs family member 1, photoreceptor morphogenesis associated (CRB1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509367

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC
TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG
GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA
CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA
GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC
TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT
GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC
AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG
AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT
TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA
TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT
TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT
ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC
CAAGATGGCC AGCATGGATT CAGCTGCCTA TGTCCATCTG GCTACACCGG GTCCCTGTGT
GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA
GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG
GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC
TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC
AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT
ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA
AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG
ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA
GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT
GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT
TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG
CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC
TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC
ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT
CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC
AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC
AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT
ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG
ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA
AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT
GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGTCCA ACCCCTGTCA CAATGGAGGT
GTTTGCCATT CCCGGTGGGA TGACTTCTCC TGTTCCTGTC CTGCCCTCAC AAGTGGGAAA
GCCTGTGAGG AGGTTCAGTG GTGTGGATTC AGCCCGTGTC CTCACGGAGC CCAGTGCCAG
CCGGTGCTTC AAGGATTTGA ATGTATTGCA AATGCTGTTT TTAATGGACA AAGCGGTCAA
ATATTATTCA GAAGCAATGG GAATATTACC AGAGAACTCA CCAATATCAC ATTTGGTTTC
AGAACAAGGG ATGCAAATGT AATAATATTG CATGCAGAAA AAGAGCCTGA ATTTCTTAAT
ATTAGCATTC AAGATTCCAG ATTATTCTTT CAATTGCAAA GTGGCAACAG CTTTTATATG
CTAAGTCTGA CAAGTTTGCA GTCAGTGAAT GATGGCACAT GGCACGAAGT GACCCTTTCC
ATGACAGACC CACTGTCCCA GACCTCCAGG TGGCAAATGG AAGTGGACAA CGAAACACCT
TTTGTGACCA GCACAATTGC TACTGGAAGC CTCAACTTTT TGAAGGATAA TACAGATATT
TATGTGGGAG ACAGAGCTAT TGACAATATA AAGGGCCTGC AAGGGTGTCT AAGTACAATA
GAAATCGGAG GCATTTATCT CTCTTACTTT GAAAATGTTC ATGGTTTCAT TAATAAACCT
CAGGAAGAGC AATTTCTCAA AATCTCTACC AATTCAGTGG TCACTGGCTG TTTGCAGTTA
AATGTCTGCA ACTCCAACCC CTGTTTGCAT GGAGGAAACT GTGAAGACAT CTATAGCTCT
TATCATTGCT CCTGTCCCTT GGGATGGTCA GGGAAACACT GTGAACTCAA CATCGATGAA
TGCTTTTCAA ACCCCTGTAT CCATGGCAAC TGCTCTGACA GAGTTGCAGC CTACCACTGC
ACATGTGAGC CTGGATACAC TGGTGTGAAC TGTGAAGTGG ATATAGACAA CTGCCAGAGT
CACCAGTGTG CAAATGGAGC CACCTGCATT AGTCATACTA ATGGCTATTC TTGCCTCTGT
TTTGGAAATT TTACAGGAAA ATTTTGCAGA CAGAGCAGAT TACCCTCAAC AGTCTGTGGG
AATGAGAAGA CAAATCTCAC TTGCTACAAT GGAGGCAACT GCACAGAGTT CCAGACTGAA
TTAAAATGTA TGTGCCGGCC AGGTTTTACT GGAGAATGTT GGCAGATGAC TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu66168
Clone ID Related Accession (Same CDS sequence) XM_011509368
Accession Version XM_011509368.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3639bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product protein crumbs homolog 1 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
TACATAAAAA GTGTAAACTG TGAATTGGAA ATTGACGAAT GTTGGTCCCA GCCTTGTTTA
AATGGTGCAA CTTGTCAGGA TGCTCTGGGG GCCTATTTCT GCGACTGTGC CCCTGGATTC
CTGGGGGATC ACTGTGAACT CAACACTGAT GAGTGTGCCA GTCAACCTTG TCTCCATGGA
GGGCTGTGTG TGGATGGAGA AAACAGATAT AGCTGTAACT GCACGGGTAG TGGATTCACA
GGGACACACT GTGAGACCTT GATGCCTCTT TGTTGGTCAA AACCTTGTCA CAATAATGCT
ACATGTGAGG ACAGTGTTGA CAATTACACT TGTCACTGCT GGCCTGGATA CACAGGTGCC
CAGTGTGAGA TCGACCTCAA TGAATGCAAT AGTAACCCCT GCCAGTCCAA TGGGGAATGT
GTGGAGCTGT CCTCAGAGAA ACAATATGGA CGCATCACTG GACTGCCTTC TTCTTTCAGC
TACCATGAAG CCTCAGGTTA TGTCTGTATC TGTCAGCCTG GATTCACAGG AATCCACTGC
GAAGAAGACG TCAATGAATG TTCTTCAAAC CCTTGCCAAA ATGGTGGTAC TTGTGAGAAC
TTGCCTGGGA ATTATACTTG CCATTGCCCA TTTGATAACC TTTCTAGAAC TTTTTATGGA
GGAAGGGACT GTTCTGATAT TCTCCTGGGC TGTACCCATC AGCAATGTCT AAATAATGGA
ACATGCATCC CTCACTTCCA AGATGGCCAG CATGGATTCA GCTGCCTATG TCCATCTGGC
TACACCGGGT CCCTGTGTGA AATCGCAACC ACACTTTCAT TTGAGGGCGA TGGCTTCCTG
TGGGTCAAAA GTGGCTCAGT GACAACCAAG GGCTCAGTTT GTAACATAGC CCTCAGGTTT
CAGACTGTTC AGCCAATGGC TCTTCTACTT TTCCGAAGCA ACAGGGATGT GTTTGTGAAG
CTGGAGCTGC TAAGTGGCTA CATTCACTTA TCAATTCAGG TCAATAATCA GTCAAAGGTG
CTTCTGTTCA TTTCCCACAA CACCAGCGAT GGAGAGTGGC ATTTCGTGGA GGTAATATTT
GCAGAGGCTG TGACCCTTAC CTTAATCGAC GACTCCTGTA AGGAGAAATG CATCGCGAAA
GCTCCTACTC CACTTGAAAG TGATCAATCA ATATGTGCTT TTCAGAACTC CTTTTTGGGT
GGTTTACCAG TGGGAATGAC CAGCAATGGT GTTGCTCTGC TTAACTTCTA TAATATGCCA
TCCACACCTT CGTTTGTAGG CTGTCTCCAA GACATTAAAA TTGATTGGAA TCACATTACC
CTGGAGAACA TCTCGTCTGG CTCATCATTA AATGTCAAGG CAGGCTGTGT GAGAAAGGAT
TGGTGTGAAA GCCAACCTTG TCAAAGCAGA GGACGCTGCA TCAACTTGTG GCTGAGTTAC
CAGTGTGACT GCCACAGGCC CTATGAAGGC CCCAACTGTC TGAGAGAGTA TGTGGCAGGC
AGATTTGGCC AGGATGACTC CACTGGTTAT GTCATCTTTA CTCTTGATGA GAGCTATGGA
GACACCATCA GCCTCTCCAT GTTTGTCCGA ACGCTTCAAC CATCAGGCTT ACTTCTAGCT
TTGGAAAACA GCACTTATCA ATATATCCGT GTCTGGCTAG AGCGCGGCAG ACTAGCAATG
CTGACTCCAA ACTCTCCCAA ATTAGTAGTA AAATTTGTTC TTAATGATGG AAATGTCCAC
TTGATATCTT TGAAAATCAA GCCATATAAA ATTGAACTGT ATCAGTCTTC ACAAAACCTA
GGATTTATTT CTGCTTCTAC GTGGAAAATC GAAAAGGGAG ATGTCATCTA CATTGGTGGC
CTACCTGACA AGCAAGAGAC TGAACTTAAT GGTGGATTCT TCAAAGGCTG TATCCAAGAT
GTAAGACTAA ACAACCAAAA TCTGGAATTC TTTCCAAATC CAACAAACAA TGCATCTCTC
AATCCAGTTC TTGTCAATGT AACCCAAGGC TGTGCTGGAG ACAACAGCTG CAAGTCCAAC
CCCTGTCACA ATGGAGGTGT TTGCCATTCC CGGTGGGATG ACTTCTCCTG TTCCTGTCCT
GCCCTCACAA GTGGGAAAGC CTGTGAGGAG GTTCAGTGGT GTGGATTCAG CCCGTGTCCT
CACGGAGCCC AGTGCCAGCC GGTGCTTCAA GGATTTGAAT GTATTGCAAA TGCTGTTTTT
AATGGACAAA GCGGTCAAAT ATTATTCAGA AGCAATGGGA ATATTACCAG AGAACTCACC
AATATCACAT TTGGTTTCAG AACAAGGGAT GCAAATGTAA TAATATTGCA TGCAGAAAAA
GAGCCTGAAT TTCTTAATAT TAGCATTCAA GATTCCAGAT TATTCTTTCA ATTGCAAAGT
GGCAACAGCT TTTATATGCT AAGTCTGACA AGTTTGCAGT CAGTGAATGA TGGCACATGG
CACGAAGTGA CCCTTTCCAT GACAGACCCA CTGTCCCAGA CCTCCAGGTG GCAAATGGAA
GTGGACAACG AAACACCTTT TGTGACCAGC ACAATTGCTA CTGGAAGCCT CAACTTTTTG
AAGGATAATA CAGATATTTA TGTGGGAGAC AGAGCTATTG ACAATATAAA GGGCCTGCAA
GGGTGTCTAA GTACAATAGA AATCGGAGGC ATTTATCTCT CTTACTTTGA AAATGTTCAT
GGTTTCATTA ATAAACCTCA GGAAGAGCAA TTTCTCAAAA TCTCTACCAA TTCAGTGGTC
ACTGGCTGTT TGCAGTTAAA TGTCTGCAAC TCCAACCCCT GTTTGCATGG AGGAAACTGT
GAAGACATCT ATAGCTCTTA TCATTGCTCC TGTCCCTTGG GATGGTCAGG GAAACACTGT
GAACTCAACA TCGATGAATG CTTTTCAAAC CCCTGTATCC ATGGCAACTG CTCTGACAGA
GTTGCAGCCT ACCACTGCAC ATGTGAGCCT GGATACACTG GTGTGAACTG TGAAGTGGAT
ATAGACAACT GCCAGAGTCA CCAGTGTGCA AATGGAGCCA CCTGCATTAG TCATACTAAT
GGCTATTCTT GCCTCTGTTT TGGAAATTTT ACAGGAAAAT TTTGCAGACA GAGCAGATTA
CCCTCAACAG TCTGTGGGAA TGAGAAGACA AATCTCACTT GCTACAATGG AGGCAACTGC
ACAGAGTTCC AGACTGAATT AAAATGTATG TGCCGGCCAG GTTTTACTGG AGAATGGTGT
GAAAAGGACA TTGATGAGTG TGCCTCTGAT CCGTGTGTCA ATGGAGGTCT GTGCCAGGAC
TTACTCAACA AATTCCAGTG CCTCTGTGAT GTTGCCTTTG CTGGCGAGCG CTGCGAGGTG
GACTTGGCAG ATGACTTGAT CTCCGACATT TTCACCACTA TTGGCTCAGT GACTGTCGCC
TTGTTACTGA TCCTCTTGCT GGCCATTGTT GCTTCTGTTG TCACCTCCAA CAAAAGGGCA
ACTCAGGGAA CCTACAGCCC CAGCCGTCAG GAGAAGGAGG GCTCCCGAGT GGAAATGTGG
AACTTGATGC CACCCCCTGC AATGGAGAGA CTGATTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011507670.1
CDS166..3804
Misc Feature(1)256..363(+)
Misc Feature(2)262..306(+)
Misc Feature(3)367..480(+)
Misc Feature(4)367..420(+)
Misc Feature(5)508..594(+)
Misc Feature(6)598..768(+)
Misc Feature(7)598..651(+)
Misc Feature(8)772..>855(+)
Misc Feature(9)772..825(+)
Misc Feature(10)1036..1530(+)
Misc Feature(11)1609..1704(+)
Misc Feature(12)1741..2157(+)
Misc Feature(13)2248..2352(+)
Misc Feature(14)2251..2295(+)
Misc Feature(15)2437..2889(+)
Misc Feature(16)3019..3108(+)
Misc Feature(17)3112..3219(+)
Misc Feature(18)3112..3162(+)
Misc Feature(19)3223..3330(+)
Misc Feature(20)3223..3276(+)
Misc Feature(21)3472..3582(+)
Misc Feature(22)3472..3525(+)
Translation

Target ORF information:

RefSeq Version XM_011509368
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens crumbs family member 1, photoreceptor morphogenesis associated (CRB1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509368

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
TACATAAAAA GTGTAAACTG TGAATTGGAA ATTGACGAAT GTTGGTCCCA GCCTTGTTTA
AATGGTGCAA CTTGTCAGGA TGCTCTGGGG GCCTATTTCT GCGACTGTGC CCCTGGATTC
CTGGGGGATC ACTGTGAACT CAACACTGAT GAGTGTGCCA GTCAACCTTG TCTCCATGGA
GGGCTGTGTG TGGATGGAGA AAACAGATAT AGCTGTAACT GCACGGGTAG TGGATTCACA
GGGACACACT GTGAGACCTT GATGCCTCTT TGTTGGTCAA AACCTTGTCA CAATAATGCT
ACATGTGAGG ACAGTGTTGA CAATTACACT TGTCACTGCT GGCCTGGATA CACAGGTGCC
CAGTGTGAGA TCGACCTCAA TGAATGCAAT AGTAACCCCT GCCAGTCCAA TGGGGAATGT
GTGGAGCTGT CCTCAGAGAA ACAATATGGA CGCATCACTG GACTGCCTTC TTCTTTCAGC
TACCATGAAG CCTCAGGTTA TGTCTGTATC TGTCAGCCTG GATTCACAGG AATCCACTGC
GAAGAAGACG TCAATGAATG TTCTTCAAAC CCTTGCCAAA ATGGTGGTAC TTGTGAGAAC
TTGCCTGGGA ATTATACTTG CCATTGCCCA TTTGATAACC TTTCTAGAAC TTTTTATGGA
GGAAGGGACT GTTCTGATAT TCTCCTGGGC TGTACCCATC AGCAATGTCT AAATAATGGA
ACATGCATCC CTCACTTCCA AGATGGCCAG CATGGATTCA GCTGCCTATG TCCATCTGGC
TACACCGGGT CCCTGTGTGA AATCGCAACC ACACTTTCAT TTGAGGGCGA TGGCTTCCTG
TGGGTCAAAA GTGGCTCAGT GACAACCAAG GGCTCAGTTT GTAACATAGC CCTCAGGTTT
CAGACTGTTC AGCCAATGGC TCTTCTACTT TTCCGAAGCA ACAGGGATGT GTTTGTGAAG
CTGGAGCTGC TAAGTGGCTA CATTCACTTA TCAATTCAGG TCAATAATCA GTCAAAGGTG
CTTCTGTTCA TTTCCCACAA CACCAGCGAT GGAGAGTGGC ATTTCGTGGA GGTAATATTT
GCAGAGGCTG TGACCCTTAC CTTAATCGAC GACTCCTGTA AGGAGAAATG CATCGCGAAA
GCTCCTACTC CACTTGAAAG TGATCAATCA ATATGTGCTT TTCAGAACTC CTTTTTGGGT
GGTTTACCAG TGGGAATGAC CAGCAATGGT GTTGCTCTGC TTAACTTCTA TAATATGCCA
TCCACACCTT CGTTTGTAGG CTGTCTCCAA GACATTAAAA TTGATTGGAA TCACATTACC
CTGGAGAACA TCTCGTCTGG CTCATCATTA AATGTCAAGG CAGGCTGTGT GAGAAAGGAT
TGGTGTGAAA GCCAACCTTG TCAAAGCAGA GGACGCTGCA TCAACTTGTG GCTGAGTTAC
CAGTGTGACT GCCACAGGCC CTATGAAGGC CCCAACTGTC TGAGAGAGTA TGTGGCAGGC
AGATTTGGCC AGGATGACTC CACTGGTTAT GTCATCTTTA CTCTTGATGA GAGCTATGGA
GACACCATCA GCCTCTCCAT GTTTGTCCGA ACGCTTCAAC CATCAGGCTT ACTTCTAGCT
TTGGAAAACA GCACTTATCA ATATATCCGT GTCTGGCTAG AGCGCGGCAG ACTAGCAATG
CTGACTCCAA ACTCTCCCAA ATTAGTAGTA AAATTTGTTC TTAATGATGG AAATGTCCAC
TTGATATCTT TGAAAATCAA GCCATATAAA ATTGAACTGT ATCAGTCTTC ACAAAACCTA
GGATTTATTT CTGCTTCTAC GTGGAAAATC GAAAAGGGAG ATGTCATCTA CATTGGTGGC
CTACCTGACA AGCAAGAGAC TGAACTTAAT GGTGGATTCT TCAAAGGCTG TATCCAAGAT
GTAAGACTAA ACAACCAAAA TCTGGAATTC TTTCCAAATC CAACAAACAA TGCATCTCTC
AATCCAGTTC TTGTCAATGT AACCCAAGGC TGTGCTGGAG ACAACAGCTG CAAGTCCAAC
CCCTGTCACA ATGGAGGTGT TTGCCATTCC CGGTGGGATG ACTTCTCCTG TTCCTGTCCT
GCCCTCACAA GTGGGAAAGC CTGTGAGGAG GTTCAGTGGT GTGGATTCAG CCCGTGTCCT
CACGGAGCCC AGTGCCAGCC GGTGCTTCAA GGATTTGAAT GTATTGCAAA TGCTGTTTTT
AATGGACAAA GCGGTCAAAT ATTATTCAGA AGCAATGGGA ATATTACCAG AGAACTCACC
AATATCACAT TTGGTTTCAG AACAAGGGAT GCAAATGTAA TAATATTGCA TGCAGAAAAA
GAGCCTGAAT TTCTTAATAT TAGCATTCAA GATTCCAGAT TATTCTTTCA ATTGCAAAGT
GGCAACAGCT TTTATATGCT AAGTCTGACA AGTTTGCAGT CAGTGAATGA TGGCACATGG
CACGAAGTGA CCCTTTCCAT GACAGACCCA CTGTCCCAGA CCTCCAGGTG GCAAATGGAA
GTGGACAACG AAACACCTTT TGTGACCAGC ACAATTGCTA CTGGAAGCCT CAACTTTTTG
AAGGATAATA CAGATATTTA TGTGGGAGAC AGAGCTATTG ACAATATAAA GGGCCTGCAA
GGGTGTCTAA GTACAATAGA AATCGGAGGC ATTTATCTCT CTTACTTTGA AAATGTTCAT
GGTTTCATTA ATAAACCTCA GGAAGAGCAA TTTCTCAAAA TCTCTACCAA TTCAGTGGTC
ACTGGCTGTT TGCAGTTAAA TGTCTGCAAC TCCAACCCCT GTTTGCATGG AGGAAACTGT
GAAGACATCT ATAGCTCTTA TCATTGCTCC TGTCCCTTGG GATGGTCAGG GAAACACTGT
GAACTCAACA TCGATGAATG CTTTTCAAAC CCCTGTATCC ATGGCAACTG CTCTGACAGA
GTTGCAGCCT ACCACTGCAC ATGTGAGCCT GGATACACTG GTGTGAACTG TGAAGTGGAT
ATAGACAACT GCCAGAGTCA CCAGTGTGCA AATGGAGCCA CCTGCATTAG TCATACTAAT
GGCTATTCTT GCCTCTGTTT TGGAAATTTT ACAGGAAAAT TTTGCAGACA GAGCAGATTA
CCCTCAACAG TCTGTGGGAA TGAGAAGACA AATCTCACTT GCTACAATGG AGGCAACTGC
ACAGAGTTCC AGACTGAATT AAAATGTATG TGCCGGCCAG GTTTTACTGG AGAATGGTGT
GAAAAGGACA TTGATGAGTG TGCCTCTGAT CCGTGTGTCA ATGGAGGTCT GTGCCAGGAC
TTACTCAACA AATTCCAGTG CCTCTGTGAT GTTGCCTTTG CTGGCGAGCG CTGCGAGGTG
GACTTGGCAG ATGACTTGAT CTCCGACATT TTCACCACTA TTGGCTCAGT GACTGTCGCC
TTGTTACTGA TCCTCTTGCT GGCCATTGTT GCTTCTGTTG TCACCTCCAA CAAAAGGGCA
ACTCAGGGAA CCTACAGCCC CAGCCGTCAG GAGAAGGAGG GCTCCCGAGT GGAAATGTGG
AACTTGATGC CACCCCCTGC AATGGAGAGA CTGATTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu66169
Clone ID Related Accession (Same CDS sequence) XM_011509369
Accession Version XM_011509369.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2664bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product protein crumbs homolog 1 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGGCTCTTC TACTTTTCCG AAGCAACAGG GATGTGTTTG TGAAGCTGGA GCTGCTAAGT 
GGCTACATTC ACTTATCAAT TCAGGTCAAT AATCAGTCAA AGGTGCTTCT GTTCATTTCC
CACAACACCA GCGATGGAGA GTGGCATTTC GTGGAGGTAA TATTTGCAGA GGCTGTGACC
CTTACCTTAA TCGACGACTC CTGTAAGGAG AAATGCATCG CGAAAGCTCC TACTCCACTT
GAAAGTGATC AATCAATATG TGCTTTTCAG AACTCCTTTT TGGGTGGTTT ACCAGTGGGA
ATGACCAGCA ATGGTGTTGC TCTGCTTAAC TTCTATAATA TGCCATCCAC ACCTTCGTTT
GTAGGCTGTC TCCAAGACAT TAAAATTGAT TGGAATCACA TTACCCTGGA GAACATCTCG
TCTGGCTCAT CATTAAATGT CAAGGCAGGC TGTGTGAGAA AGGATTGGTG TGAAAGCCAA
CCTTGTCAAA GCAGAGGACG CTGCATCAAC TTGTGGCTGA GTTACCAGTG TGACTGCCAC
AGGCCCTATG AAGGCCCCAA CTGTCTGAGA GAGTATGTGG CAGGCAGATT TGGCCAGGAT
GACTCCACTG GTTATGTCAT CTTTACTCTT GATGAGAGCT ATGGAGACAC CATCAGCCTC
TCCATGTTTG TCCGAACGCT TCAACCATCA GGCTTACTTC TAGCTTTGGA AAACAGCACT
TATCAATATA TCCGTGTCTG GCTAGAGCGC GGCAGACTAG CAATGCTGAC TCCAAACTCT
CCCAAATTAG TAGTAAAATT TGTTCTTAAT GATGGAAATG TCCACTTGAT ATCTTTGAAA
ATCAAGCCAT ATAAAATTGA ACTGTATCAG TCTTCACAAA ACCTAGGATT TATTTCTGCT
TCTACGTGGA AAATCGAAAA GGGAGATGTC ATCTACATTG GTGGCCTACC TGACAAGCAA
GAGACTGAAC TTAATGGTGG ATTCTTCAAA GGCTGTATCC AAGATGTAAG ACTAAACAAC
CAAAATCTGG AATTCTTTCC AAATCCAACA AACAATGCAT CTCTCAATCC AGTTCTTGTC
AATGTAACCC AAGGCTGTGC TGGAGACAAC AGCTGCAAGT CCAACCCCTG TCACAATGGA
GGTGTTTGCC ATTCCCGGTG GGATGACTTC TCCTGTTCCT GTCCTGCCCT CACAAGTGGG
AAAGCCTGTG AGGAGGTTCA GTGGTGTGGA TTCAGCCCGT GTCCTCACGG AGCCCAGTGC
CAGCCGGTGC TTCAAGGATT TGAATGTATT GCAAATGCTG TTTTTAATGG ACAAAGCGGT
CAAATATTAT TCAGAAGCAA TGGGAATATT ACCAGAGAAC TCACCAATAT CACATTTGGT
TTCAGAACAA GGGATGCAAA TGTAATAATA TTGCATGCAG AAAAAGAGCC TGAATTTCTT
AATATTAGCA TTCAAGATTC CAGATTATTC TTTCAATTGC AAAGTGGCAA CAGCTTTTAT
ATGCTAAGTC TGACAAGTTT GCAGTCAGTG AATGATGGCA CATGGCACGA AGTGACCCTT
TCCATGACAG ACCCACTGTC CCAGACCTCC AGGTGGCAAA TGGAAGTGGA CAACGAAACA
CCTTTTGTGA CCAGCACAAT TGCTACTGGA AGCCTCAACT TTTTGAAGGA TAATACAGAT
ATTTATGTGG GAGACAGAGC TATTGACAAT ATAAAGGGCC TGCAAGGGTG TCTAAGTACA
ATAGAAATCG GAGGCATTTA TCTCTCTTAC TTTGAAAATG TTCATGGTTT CATTAATAAA
CCTCAGGAAG AGCAATTTCT CAAAATCTCT ACCAATTCAG TGGTCACTGG CTGTTTGCAG
TTAAATGTCT GCAACTCCAA CCCCTGTTTG CATGGAGGAA ACTGTGAAGA CATCTATAGC
TCTTATCATT GCTCCTGTCC CTTGGGATGG TCAGGGAAAC ACTGTGAACT CAACATCGAT
GAATGCTTTT CAAACCCCTG TATCCATGGC AACTGCTCTG ACAGAGTTGC AGCCTACCAC
TGCACATGTG AGCCTGGATA CACTGGTGTG AACTGTGAAG TGGATATAGA CAACTGCCAG
AGTCACCAGT GTGCAAATGG AGCCACCTGC ATTAGTCATA CTAATGGCTA TTCTTGCCTC
TGTTTTGGAA ATTTTACAGG AAAATTTTGC AGACAGAGCA GATTACCCTC AACAGTCTGT
GGGAATGAGA AGACAAATCT CACTTGCTAC AATGGAGGCA ACTGCACAGA GTTCCAGACT
GAATTAAAAT GTATGTGCCG GCCAGGTTTT ACTGGAGAAT GGTGTGAAAA GGACATTGAT
GAGTGTGCCT CTGATCCGTG TGTCAATGGA GGTCTGTGCC AGGACTTACT CAACAAATTC
CAGTGCCTCT GTGATGTTGC CTTTGCTGGC GAGCGCTGCG AGGTGGACTT GGCAGATGAC
TTGATCTCCG ACATTTTCAC CACTATTGGC TCAGTGACTG TCGCCTTGTT ACTGATCCTC
TTGCTGGCCA TTGTTGCTTC TGTTGTCACC TCCAACAAAA GGGCAACTCA GGGAACCTAC
AGCCCCAGCC GTCAGGAGAA GGAGGGCTCC CGAGTGGAAA TGTGGAACTT GATGCCACCC
CCTGCAATGG AGAGACTGAT TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011507671.1
CDS674..3337
Misc Feature(1)677..1063(+)
Misc Feature(2)1142..1237(+)
Misc Feature(3)1274..1690(+)
Misc Feature(4)1781..1885(+)
Misc Feature(5)1784..1828(+)
Misc Feature(6)1970..2422(+)
Misc Feature(7)2552..2641(+)
Misc Feature(8)2645..2752(+)
Misc Feature(9)2645..2695(+)
Misc Feature(10)2756..2863(+)
Misc Feature(11)2756..2809(+)
Misc Feature(12)3005..3115(+)
Misc Feature(13)3005..3058(+)
Translation

Target ORF information:

RefSeq Version XM_011509369
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens crumbs family member 1, photoreceptor morphogenesis associated (CRB1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509369

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGGCTCTTC TACTTTTCCG AAGCAACAGG GATGTGTTTG TGAAGCTGGA GCTGCTAAGT 
GGCTACATTC ACTTATCAAT TCAGGTCAAT AATCAGTCAA AGGTGCTTCT GTTCATTTCC
CACAACACCA GCGATGGAGA GTGGCATTTC GTGGAGGTAA TATTTGCAGA GGCTGTGACC
CTTACCTTAA TCGACGACTC CTGTAAGGAG AAATGCATCG CGAAAGCTCC TACTCCACTT
GAAAGTGATC AATCAATATG TGCTTTTCAG AACTCCTTTT TGGGTGGTTT ACCAGTGGGA
ATGACCAGCA ATGGTGTTGC TCTGCTTAAC TTCTATAATA TGCCATCCAC ACCTTCGTTT
GTAGGCTGTC TCCAAGACAT TAAAATTGAT TGGAATCACA TTACCCTGGA GAACATCTCG
TCTGGCTCAT CATTAAATGT CAAGGCAGGC TGTGTGAGAA AGGATTGGTG TGAAAGCCAA
CCTTGTCAAA GCAGAGGACG CTGCATCAAC TTGTGGCTGA GTTACCAGTG TGACTGCCAC
AGGCCCTATG AAGGCCCCAA CTGTCTGAGA GAGTATGTGG CAGGCAGATT TGGCCAGGAT
GACTCCACTG GTTATGTCAT CTTTACTCTT GATGAGAGCT ATGGAGACAC CATCAGCCTC
TCCATGTTTG TCCGAACGCT TCAACCATCA GGCTTACTTC TAGCTTTGGA AAACAGCACT
TATCAATATA TCCGTGTCTG GCTAGAGCGC GGCAGACTAG CAATGCTGAC TCCAAACTCT
CCCAAATTAG TAGTAAAATT TGTTCTTAAT GATGGAAATG TCCACTTGAT ATCTTTGAAA
ATCAAGCCAT ATAAAATTGA ACTGTATCAG TCTTCACAAA ACCTAGGATT TATTTCTGCT
TCTACGTGGA AAATCGAAAA GGGAGATGTC ATCTACATTG GTGGCCTACC TGACAAGCAA
GAGACTGAAC TTAATGGTGG ATTCTTCAAA GGCTGTATCC AAGATGTAAG ACTAAACAAC
CAAAATCTGG AATTCTTTCC AAATCCAACA AACAATGCAT CTCTCAATCC AGTTCTTGTC
AATGTAACCC AAGGCTGTGC TGGAGACAAC AGCTGCAAGT CCAACCCCTG TCACAATGGA
GGTGTTTGCC ATTCCCGGTG GGATGACTTC TCCTGTTCCT GTCCTGCCCT CACAAGTGGG
AAAGCCTGTG AGGAGGTTCA GTGGTGTGGA TTCAGCCCGT GTCCTCACGG AGCCCAGTGC
CAGCCGGTGC TTCAAGGATT TGAATGTATT GCAAATGCTG TTTTTAATGG ACAAAGCGGT
CAAATATTAT TCAGAAGCAA TGGGAATATT ACCAGAGAAC TCACCAATAT CACATTTGGT
TTCAGAACAA GGGATGCAAA TGTAATAATA TTGCATGCAG AAAAAGAGCC TGAATTTCTT
AATATTAGCA TTCAAGATTC CAGATTATTC TTTCAATTGC AAAGTGGCAA CAGCTTTTAT
ATGCTAAGTC TGACAAGTTT GCAGTCAGTG AATGATGGCA CATGGCACGA AGTGACCCTT
TCCATGACAG ACCCACTGTC CCAGACCTCC AGGTGGCAAA TGGAAGTGGA CAACGAAACA
CCTTTTGTGA CCAGCACAAT TGCTACTGGA AGCCTCAACT TTTTGAAGGA TAATACAGAT
ATTTATGTGG GAGACAGAGC TATTGACAAT ATAAAGGGCC TGCAAGGGTG TCTAAGTACA
ATAGAAATCG GAGGCATTTA TCTCTCTTAC TTTGAAAATG TTCATGGTTT CATTAATAAA
CCTCAGGAAG AGCAATTTCT CAAAATCTCT ACCAATTCAG TGGTCACTGG CTGTTTGCAG
TTAAATGTCT GCAACTCCAA CCCCTGTTTG CATGGAGGAA ACTGTGAAGA CATCTATAGC
TCTTATCATT GCTCCTGTCC CTTGGGATGG TCAGGGAAAC ACTGTGAACT CAACATCGAT
GAATGCTTTT CAAACCCCTG TATCCATGGC AACTGCTCTG ACAGAGTTGC AGCCTACCAC
TGCACATGTG AGCCTGGATA CACTGGTGTG AACTGTGAAG TGGATATAGA CAACTGCCAG
AGTCACCAGT GTGCAAATGG AGCCACCTGC ATTAGTCATA CTAATGGCTA TTCTTGCCTC
TGTTTTGGAA ATTTTACAGG AAAATTTTGC AGACAGAGCA GATTACCCTC AACAGTCTGT
GGGAATGAGA AGACAAATCT CACTTGCTAC AATGGAGGCA ACTGCACAGA GTTCCAGACT
GAATTAAAAT GTATGTGCCG GCCAGGTTTT ACTGGAGAAT GGTGTGAAAA GGACATTGAT
GAGTGTGCCT CTGATCCGTG TGTCAATGGA GGTCTGTGCC AGGACTTACT CAACAAATTC
CAGTGCCTCT GTGATGTTGC CTTTGCTGGC GAGCGCTGCG AGGTGGACTT GGCAGATGAC
TTGATCTCCG ACATTTTCAC CACTATTGGC TCAGTGACTG TCGCCTTGTT ACTGATCCTC
TTGCTGGCCA TTGTTGCTTC TGTTGTCACC TCCAACAAAA GGGCAACTCA GGGAACCTAC
AGCCCCAGCC GTCAGGAGAA GGAGGGCTCC CGAGTGGAAA TGTGGAACTT GATGCCACCC
CCTGCAATGG AGAGACTGAT TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19399
Clone ID Related Accession (Same CDS sequence) NM_201253
Accession Version NM_201253.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4221bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product protein crumbs homolog 1 isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC370545.1, DC370777.1, BC136271.1 and AY043325.1. This sequence is a reference standard in the RefSeqGene project. On Aug 7, 2010 this sequence version replaced gi:41327707. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY043325.1, AK289733.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC
TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG
GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA
CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA
GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC
TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT
GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC
AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG
AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT
TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA
TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT
TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT
ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC
CAAGATGGCC AGCATGGATT CAGCTGCCTG TGTCCATCTG GCTACACCGG GTCCCTGTGT
GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA
GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG
GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC
TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC
AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT
ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA
AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG
ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA
GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT
GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT
TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG
CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC
TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC
ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT
CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC
AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC
AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT
ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG
ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA
AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT
GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGTCCA ACCCCTGTCA CAATGGAGGT
GTTTGCCATT CCCGGTGGGA TGACTTCTCC TGTTCCTGTC CTGCCCTCAC AAGTGGGAAA
GCCTGTGAGG AGGTTCAGTG GTGTGGATTC AGCCCGTGTC CTCACGGAGC CCAGTGCCAG
CCGGTGCTTC AAGGATTTGA ATGTATTGCA AATGCTGTTT TTAATGGACA AAGCGGTCAA
ATATTATTCA GAAGCAATGG GAATATTACC AGAGAACTCA CCAATATCAC ATTTGGTTTC
AGAACAAGGG ATGCAAATGT AATAATATTG CATGCAGAAA AAGAGCCTGA ATTTCTTAAT
ATTAGCATTC AAGATTCCAG ATTATTCTTT CAATTGCAAA GTGGCAACAG CTTTTATATG
CTAAGTCTGA CAAGTTTGCA GTCAGTGAAT GATGGCACAT GGCACGAAGT GACCCTTTCC
ATGACAGACC CACTGTCCCA GACCTCCAGG TGGCAAATGG AAGTGGACAA CGAAACACCT
TTTGTGACCA GCACAATTGC TACTGGAAGC CTCAACTTTT TGAAGGATAA TACAGATATT
TATGTGGGAG ACAGAGCTAT TGACAATATA AAGGGCCTGC AAGGGTGTCT AAGTACAATA
GAAATCGGAG GCATTTATCT CTCTTACTTT GAAAATGTTC ATGGTTTCAT TAATAAACCT
CAGGAAGAGC AATTTCTCAA AATCTCTACC AATTCAGTGG TCACTGGCTG TTTGCAGTTA
AATGTCTGCA ACTCCAACCC CTGTTTGCAT GGAGGAAACT GTGAAGACAT CTATAGCTCT
TATCATTGCT CCTGTCCCTT GGGATGGTCA GGGAAACACT GTGAACTCAA CATCGATGAA
TGCTTTTCAA ACCCCTGTAT CCATGGCAAC TGCTCTGACA GAGTTGCAGC CTACCACTGC
ACATGTGAGC CTGGATACAC TGGTGTGAAC TGTGAAGTGG ATATAGACAA CTGCCAGAGT
CACCAGTGTG CAAATGGAGC CACCTGCATT AGTCATACTA ATGGCTATTC TTGCCTCTGT
TTTGGAAATT TTACAGGAAA ATTTTGCAGA CAGAGCAGAT TACCCTCAAC AGTCTGTGGG
AATGAGAAGA CAAATCTCAC TTGCTACAAT GGAGGCAACT GCACAGAGTT CCAGACTGAA
TTAAAATGTA TGTGCCGGCC AGGTTTTACT GGAGAATGGT GTGAAAAGGA CATTGATGAG
TGTGCCTCTG ATCCGTGTGT CAATGGAGGT CTGTGCCAGG ACTTACTCAA CAAATTCCAG
TGCCTCTGTG ATGTTGCCTT TGCTGGCGAG CGCTGCGAGG TGGACTTGGC AGATGACTTG
ATCTCCGACA TTTTCACCAC TATTGGCTCA GTGACTGTCG CCTTGTTACT GATCCTCTTG
CTGGCCATTG TTGCTTCTGT TGTCACCTCC AACAAAAGGG CAACTCAGGG AACCTACAGC
CCCAGCCGTC AGGAGAAGGA GGGCTCCCGA GTGGAAATGT GGAACTTGAT GCCACCCCCT
GCAATGGAGA GACTGATTTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_957705.1
CDS210..4430
Misc Feature(1)132..134(+)
Misc Feature(2)423..533(+)
Misc Feature(3)426..470(+)
Misc Feature(4)651..758(+)
Misc Feature(5)651..704(+)
Misc Feature(6)768..875(+)
Misc Feature(7)774..818(+)
Misc Feature(8)882..989(+)
Misc Feature(9)888..932(+)
Misc Feature(10)993..1106(+)
Misc Feature(11)993..1046(+)
Misc Feature(12)1134..1220(+)
Misc Feature(13)1398..>1481(+)
Misc Feature(14)1398..1451(+)
Misc Feature(15)1662..2156(+)
Misc Feature(16)2235..2330(+)
Misc Feature(17)2367..2783(+)
Misc Feature(18)2874..2978(+)
Misc Feature(19)2877..2921(+)
Misc Feature(20)3063..3515(+)
Misc Feature(21)3645..3734(+)
Misc Feature(22)3738..3845(+)
Misc Feature(23)3738..3788(+)
Misc Feature(24)3849..3956(+)
Misc Feature(25)3849..3902(+)
Misc Feature(26)4098..4208(+)
Misc Feature(27)4098..4151(+)
Misc Feature(28)4251..4313(+)
Exon (1)1..279
Gene:CRB1
Gene Synonym:
Exon (2)280..861
Gene:CRB1
Gene Synonym:
Exon (3)862..1057
Gene:CRB1
Gene Synonym:
Exon (4)1058..1197
Gene:CRB1
Gene Synonym:
Exon (5)1198..1380
Gene:CRB1
Gene Synonym:
Exon (6)1381..2337
Gene:CRB1
Gene Synonym:
Exon (7)2338..2885
Gene:CRB1
Gene Synonym:
Exon (8)2886..3051
Gene:CRB1
Gene Synonym:
Exon (9)3052..3958
Gene:CRB1
Gene Synonym:
Exon (10)3959..4087
Gene:CRB1
Gene Synonym:
Exon (11)4088..4214
Gene:CRB1
Gene Synonym:
Exon (12)4215..5006
Gene:CRB1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_201253
Organism Homo sapiens (human)
Definition Homo sapiens crumbs family member 1, photoreceptor morphogenesis associated (CRB1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_201253

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC
TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG
GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA
CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA
GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC
TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT
GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC
AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG
AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT
TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA
TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT
TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT
ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC
CAAGATGGCC AGCATGGATT CAGCTGCCTG TGTCCATCTG GCTACACCGG GTCCCTGTGT
GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA
GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG
GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC
TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC
AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT
ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA
AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG
ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA
GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT
GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT
TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG
CCCTATGAAG GCCCCAACTG TCTGAGAGAG TATGTGGCAG GCAGATTTGG CCAGGATGAC
TCCACTGGTT ATGTCATCTT TACTCTTGAT GAGAGCTATG GAGACACCAT CAGCCTCTCC
ATGTTTGTCC GAACGCTTCA ACCATCAGGC TTACTTCTAG CTTTGGAAAA CAGCACTTAT
CAATATATCC GTGTCTGGCT AGAGCGCGGC AGACTAGCAA TGCTGACTCC AAACTCTCCC
AAATTAGTAG TAAAATTTGT TCTTAATGAT GGAAATGTCC ACTTGATATC TTTGAAAATC
AAGCCATATA AAATTGAACT GTATCAGTCT TCACAAAACC TAGGATTTAT TTCTGCTTCT
ACGTGGAAAA TCGAAAAGGG AGATGTCATC TACATTGGTG GCCTACCTGA CAAGCAAGAG
ACTGAACTTA ATGGTGGATT CTTCAAAGGC TGTATCCAAG ATGTAAGACT AAACAACCAA
AATCTGGAAT TCTTTCCAAA TCCAACAAAC AATGCATCTC TCAATCCAGT TCTTGTCAAT
GTAACCCAAG GCTGTGCTGG AGACAACAGC TGCAAGTCCA ACCCCTGTCA CAATGGAGGT
GTTTGCCATT CCCGGTGGGA TGACTTCTCC TGTTCCTGTC CTGCCCTCAC AAGTGGGAAA
GCCTGTGAGG AGGTTCAGTG GTGTGGATTC AGCCCGTGTC CTCACGGAGC CCAGTGCCAG
CCGGTGCTTC AAGGATTTGA ATGTATTGCA AATGCTGTTT TTAATGGACA AAGCGGTCAA
ATATTATTCA GAAGCAATGG GAATATTACC AGAGAACTCA CCAATATCAC ATTTGGTTTC
AGAACAAGGG ATGCAAATGT AATAATATTG CATGCAGAAA AAGAGCCTGA ATTTCTTAAT
ATTAGCATTC AAGATTCCAG ATTATTCTTT CAATTGCAAA GTGGCAACAG CTTTTATATG
CTAAGTCTGA CAAGTTTGCA GTCAGTGAAT GATGGCACAT GGCACGAAGT GACCCTTTCC
ATGACAGACC CACTGTCCCA GACCTCCAGG TGGCAAATGG AAGTGGACAA CGAAACACCT
TTTGTGACCA GCACAATTGC TACTGGAAGC CTCAACTTTT TGAAGGATAA TACAGATATT
TATGTGGGAG ACAGAGCTAT TGACAATATA AAGGGCCTGC AAGGGTGTCT AAGTACAATA
GAAATCGGAG GCATTTATCT CTCTTACTTT GAAAATGTTC ATGGTTTCAT TAATAAACCT
CAGGAAGAGC AATTTCTCAA AATCTCTACC AATTCAGTGG TCACTGGCTG TTTGCAGTTA
AATGTCTGCA ACTCCAACCC CTGTTTGCAT GGAGGAAACT GTGAAGACAT CTATAGCTCT
TATCATTGCT CCTGTCCCTT GGGATGGTCA GGGAAACACT GTGAACTCAA CATCGATGAA
TGCTTTTCAA ACCCCTGTAT CCATGGCAAC TGCTCTGACA GAGTTGCAGC CTACCACTGC
ACATGTGAGC CTGGATACAC TGGTGTGAAC TGTGAAGTGG ATATAGACAA CTGCCAGAGT
CACCAGTGTG CAAATGGAGC CACCTGCATT AGTCATACTA ATGGCTATTC TTGCCTCTGT
TTTGGAAATT TTACAGGAAA ATTTTGCAGA CAGAGCAGAT TACCCTCAAC AGTCTGTGGG
AATGAGAAGA CAAATCTCAC TTGCTACAAT GGAGGCAACT GCACAGAGTT CCAGACTGAA
TTAAAATGTA TGTGCCGGCC AGGTTTTACT GGAGAATGGT GTGAAAAGGA CATTGATGAG
TGTGCCTCTG ATCCGTGTGT CAATGGAGGT CTGTGCCAGG ACTTACTCAA CAAATTCCAG
TGCCTCTGTG ATGTTGCCTT TGCTGGCGAG CGCTGCGAGG TGGACTTGGC AGATGACTTG
ATCTCCGACA TTTTCACCAC TATTGGCTCA GTGACTGTCG CCTTGTTACT GATCCTCTTG
CTGGCCATTG TTGCTTCTGT TGTCACCTCC AACAAAAGGG CAACTCAGGG AACCTACAGC
CCCAGCCGTC AGGAGAAGGA GGGCTCCCGA GTGGAAATGT GGAACTTGAT GCCACCCCCT
GCAATGGAGA GACTGATTTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17475
Clone ID Related Accession (Same CDS sequence) NM_001193640
Accession Version NM_001193640.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3885bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product protein crumbs homolog 1 isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC370545.1, DC370777.1, AJ748821.1 and AY043325.1. Transcript Variant: This variant (2) lacks two in-frame exons compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ748821.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157437, SAMEA962344 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGATACACA
GGTGCCCAGT GTGAGATCGA CCTCAATGAA TGCAATAGTA ACCCCTGCCA GTCCAATGGG
GAATGTGTGG AGCTGTCCTC AGAGAAACAA TATGGACGCA TCACTGGACT GCCTTCTTCT
TTCAGCTACC ATGAAGCCTC AGGTTATGTC TGTATCTGTC AGCCTGGATT CACAGGAATC
CACTGCGAAG AAGACGTCAA TGAATGTTCT TCAAACCCTT GCCAAAATGG TGGTACTTGT
GAGAACTTGC CTGGGAATTA TACTTGCCAT TGCCCATTTG ATAACCTTTC TAGAACTTTT
TATGGAGGAA GGGACTGTTC TGATATTCTC CTGGGCTGTA CCCATCAGCA ATGTCTAAAT
AATGGAACAT GCATCCCTCA CTTCCAAGAT GGCCAGCATG GATTCAGCTG CCTGTGTCCA
TCTGGCTACA CCGGGTCCCT GTGTGAAATC GCAACCACAC TTTCATTTGA GGGCGATGGC
TTCCTGTGGG TCAAAAGTGG CTCAGTGACA ACCAAGGGCT CAGTTTGTAA CATAGCCCTC
AGGTTTCAGA CTGTTCAGCC AATGGCTCTT CTACTTTTCC GAAGCAACAG GGATGTGTTT
GTGAAGCTGG AGCTGCTAAG TGGCTACATT CACTTATCAA TTCAGGTCAA TAATCAGTCA
AAGGTGCTTC TGTTCATTTC CCACAACACC AGCGATGGAG AGTGGCATTT CGTGGAGGTA
ATATTTGCAG AGGCTGTGAC CCTTACCTTA ATCGACGACT CCTGTAAGGA GAAATGCATC
GCGAAAGCTC CTACTCCACT TGAAAGTGAT CAATCAATAT GTGCTTTTCA GAACTCCTTT
TTGGGTGGTT TACCAGTGGG AATGACCAGC AATGGTGTTG CTCTGCTTAA CTTCTATAAT
ATGCCATCCA CACCTTCGTT TGTAGGCTGT CTCCAAGACA TTAAAATTGA TTGGAATCAC
ATTACCCTGG AGAACATCTC GTCTGGCTCA TCATTAAATG TCAAGGCAGG CTGTGTGAGA
AAGGATTGGT GTGAAAGCCA ACCTTGTCAA AGCAGAGGAC GCTGCATCAA CTTGTGGCTG
AGTTACCAGT GTGACTGCCA CAGGCCCTAT GAAGGCCCCA ACTGTCTGAG AGAGTATGTG
GCAGGCAGAT TTGGCCAGGA TGACTCCACT GGTTATGTCA TCTTTACTCT TGATGAGAGC
TATGGAGACA CCATCAGCCT CTCCATGTTT GTCCGAACGC TTCAACCATC AGGCTTACTT
CTAGCTTTGG AAAACAGCAC TTATCAATAT ATCCGTGTCT GGCTAGAGCG CGGCAGACTA
GCAATGCTGA CTCCAAACTC TCCCAAATTA GTAGTAAAAT TTGTTCTTAA TGATGGAAAT
GTCCACTTGA TATCTTTGAA AATCAAGCCA TATAAAATTG AACTGTATCA GTCTTCACAA
AACCTAGGAT TTATTTCTGC TTCTACGTGG AAAATCGAAA AGGGAGATGT CATCTACATT
GGTGGCCTAC CTGACAAGCA AGAGACTGAA CTTAATGGTG GATTCTTCAA AGGCTGTATC
CAAGATGTAA GACTAAACAA CCAAAATCTG GAATTCTTTC CAAATCCAAC AAACAATGCA
TCTCTCAATC CAGTTCTTGT CAATGTAACC CAAGGCTGTG CTGGAGACAA CAGCTGCAAG
TCCAACCCCT GTCACAATGG AGGTGTTTGC CATTCCCGGT GGGATGACTT CTCCTGTTCC
TGTCCTGCCC TCACAAGTGG GAAAGCCTGT GAGGAGGTTC AGTGGTGTGG ATTCAGCCCG
TGTCCTCACG GAGCCCAGTG CCAGCCGGTG CTTCAAGGAT TTGAATGTAT TGCAAATGCT
GTTTTTAATG GACAAAGCGG TCAAATATTA TTCAGAAGCA ATGGGAATAT TACCAGAGAA
CTCACCAATA TCACATTTGG TTTCAGAACA AGGGATGCAA ATGTAATAAT ATTGCATGCA
GAAAAAGAGC CTGAATTTCT TAATATTAGC ATTCAAGATT CCAGATTATT CTTTCAATTG
CAAAGTGGCA ACAGCTTTTA TATGCTAAGT CTGACAAGTT TGCAGTCAGT GAATGATGGC
ACATGGCACG AAGTGACCCT TTCCATGACA GACCCACTGT CCCAGACCTC CAGGTGGCAA
ATGGAAGTGG ACAACGAAAC ACCTTTTGTG ACCAGCACAA TTGCTACTGG AAGCCTCAAC
TTTTTGAAGG ATAATACAGA TATTTATGTG GGAGACAGAG CTATTGACAA TATAAAGGGC
CTGCAAGGGT GTCTAAGTAC AATAGAAATC GGAGGCATTT ATCTCTCTTA CTTTGAAAAT
GTTCATGGTT TCATTAATAA ACCTCAGGAA GAGCAATTTC TCAAAATCTC TACCAATTCA
GTGGTCACTG GCTGTTTGCA GTTAAATGTC TGCAACTCCA ACCCCTGTTT GCATGGAGGA
AACTGTGAAG ACATCTATAG CTCTTATCAT TGCTCCTGTC CCTTGGGATG GTCAGGGAAA
CACTGTGAAC TCAACATCGA TGAATGCTTT TCAAACCCCT GTATCCATGG CAACTGCTCT
GACAGAGTTG CAGCCTACCA CTGCACATGT GAGCCTGGAT ACACTGGTGT GAACTGTGAA
GTGGATATAG ACAACTGCCA GAGTCACCAG TGTGCAAATG GAGCCACCTG CATTAGTCAT
ACTAATGGCT ATTCTTGCCT CTGTTTTGGA AATTTTACAG GAAAATTTTG CAGACAGAGC
AGATTACCCT CAACAGTCTG TGGGAATGAG AAGACAAATC TCACTTGCTA CAATGGAGGC
AACTGCACAG AGTTCCAGAC TGAATTAAAA TGTATGTGCC GGCCAGGTTT TACTGGAGAA
TGGTGTGAAA AGGACATTGA TGAGTGTGCC TCTGATCCGT GTGTCAATGG AGGTCTGTGC
CAGGACTTAC TCAACAAATT CCAGTGCCTC TGTGATGTTG CCTTTGCTGG CGAGCGCTGC
GAGGTGGACT TGGCAGATGA CTTGATCTCC GACATTTTCA CCACTATTGG CTCAGTGACT
GTCGCCTTGT TACTGATCCT CTTGCTGGCC ATTGTTGCTT CTGTTGTCAC CTCCAACAAA
AGGGCAACTC AGGGAACCTA CAGCCCCAGC CGTCAGGAGA AGGAGGGCTC CCGAGTGGAA
ATGTGGAACT TGATGCCACC CCCTGCAATG GAGAGACTGA TTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001180569.1
CDS210..4094
Misc Feature(1)132..134(+)
Misc Feature(2)423..533(+)
Misc Feature(3)426..470(+)
Misc Feature(4)651..758(+)
Misc Feature(5)651..704(+)
Misc Feature(6)768..884(+)
Misc Feature(7)774..818(+)
Misc Feature(8)888..1058(+)
Misc Feature(9)888..941(+)
Misc Feature(10)1062..>1145(+)
Misc Feature(11)1062..1115(+)
Misc Feature(12)1326..1820(+)
Misc Feature(13)1899..1994(+)
Misc Feature(14)2031..2447(+)
Misc Feature(15)2538..2642(+)
Misc Feature(16)2541..2585(+)
Misc Feature(17)2727..3179(+)
Misc Feature(18)3309..3398(+)
Misc Feature(19)3402..3509(+)
Misc Feature(20)3402..3452(+)
Misc Feature(21)3513..3620(+)
Misc Feature(22)3513..3566(+)
Misc Feature(23)3762..3872(+)
Misc Feature(24)3762..3815(+)
Exon (1)1..279
Gene:CRB1
Gene Synonym:
Exon (2)280..861
Gene:CRB1
Gene Synonym:
Exon (3)862..1044
Gene:CRB1
Gene Synonym:
Exon (4)1045..2001
Gene:CRB1
Gene Synonym:
Exon (5)2002..2549
Gene:CRB1
Gene Synonym:
Exon (6)2550..2715
Gene:CRB1
Gene Synonym:
Exon (7)2716..3622
Gene:CRB1
Gene Synonym:
Exon (8)3623..3751
Gene:CRB1
Gene Synonym:
Exon (9)3752..3878
Gene:CRB1
Gene Synonym:
Exon (10)3879..4670
Gene:CRB1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001193640
Organism Homo sapiens (human)
Definition Homo sapiens crumbs family member 1, photoreceptor morphogenesis associated (CRB1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001193640

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGATACACA
GGTGCCCAGT GTGAGATCGA CCTCAATGAA TGCAATAGTA ACCCCTGCCA GTCCAATGGG
GAATGTGTGG AGCTGTCCTC AGAGAAACAA TATGGACGCA TCACTGGACT GCCTTCTTCT
TTCAGCTACC ATGAAGCCTC AGGTTATGTC TGTATCTGTC AGCCTGGATT CACAGGAATC
CACTGCGAAG AAGACGTCAA TGAATGTTCT TCAAACCCTT GCCAAAATGG TGGTACTTGT
GAGAACTTGC CTGGGAATTA TACTTGCCAT TGCCCATTTG ATAACCTTTC TAGAACTTTT
TATGGAGGAA GGGACTGTTC TGATATTCTC CTGGGCTGTA CCCATCAGCA ATGTCTAAAT
AATGGAACAT GCATCCCTCA CTTCCAAGAT GGCCAGCATG GATTCAGCTG CCTGTGTCCA
TCTGGCTACA CCGGGTCCCT GTGTGAAATC GCAACCACAC TTTCATTTGA GGGCGATGGC
TTCCTGTGGG TCAAAAGTGG CTCAGTGACA ACCAAGGGCT CAGTTTGTAA CATAGCCCTC
AGGTTTCAGA CTGTTCAGCC AATGGCTCTT CTACTTTTCC GAAGCAACAG GGATGTGTTT
GTGAAGCTGG AGCTGCTAAG TGGCTACATT CACTTATCAA TTCAGGTCAA TAATCAGTCA
AAGGTGCTTC TGTTCATTTC CCACAACACC AGCGATGGAG AGTGGCATTT CGTGGAGGTA
ATATTTGCAG AGGCTGTGAC CCTTACCTTA ATCGACGACT CCTGTAAGGA GAAATGCATC
GCGAAAGCTC CTACTCCACT TGAAAGTGAT CAATCAATAT GTGCTTTTCA GAACTCCTTT
TTGGGTGGTT TACCAGTGGG AATGACCAGC AATGGTGTTG CTCTGCTTAA CTTCTATAAT
ATGCCATCCA CACCTTCGTT TGTAGGCTGT CTCCAAGACA TTAAAATTGA TTGGAATCAC
ATTACCCTGG AGAACATCTC GTCTGGCTCA TCATTAAATG TCAAGGCAGG CTGTGTGAGA
AAGGATTGGT GTGAAAGCCA ACCTTGTCAA AGCAGAGGAC GCTGCATCAA CTTGTGGCTG
AGTTACCAGT GTGACTGCCA CAGGCCCTAT GAAGGCCCCA ACTGTCTGAG AGAGTATGTG
GCAGGCAGAT TTGGCCAGGA TGACTCCACT GGTTATGTCA TCTTTACTCT TGATGAGAGC
TATGGAGACA CCATCAGCCT CTCCATGTTT GTCCGAACGC TTCAACCATC AGGCTTACTT
CTAGCTTTGG AAAACAGCAC TTATCAATAT ATCCGTGTCT GGCTAGAGCG CGGCAGACTA
GCAATGCTGA CTCCAAACTC TCCCAAATTA GTAGTAAAAT TTGTTCTTAA TGATGGAAAT
GTCCACTTGA TATCTTTGAA AATCAAGCCA TATAAAATTG AACTGTATCA GTCTTCACAA
AACCTAGGAT TTATTTCTGC TTCTACGTGG AAAATCGAAA AGGGAGATGT CATCTACATT
GGTGGCCTAC CTGACAAGCA AGAGACTGAA CTTAATGGTG GATTCTTCAA AGGCTGTATC
CAAGATGTAA GACTAAACAA CCAAAATCTG GAATTCTTTC CAAATCCAAC AAACAATGCA
TCTCTCAATC CAGTTCTTGT CAATGTAACC CAAGGCTGTG CTGGAGACAA CAGCTGCAAG
TCCAACCCCT GTCACAATGG AGGTGTTTGC CATTCCCGGT GGGATGACTT CTCCTGTTCC
TGTCCTGCCC TCACAAGTGG GAAAGCCTGT GAGGAGGTTC AGTGGTGTGG ATTCAGCCCG
TGTCCTCACG GAGCCCAGTG CCAGCCGGTG CTTCAAGGAT TTGAATGTAT TGCAAATGCT
GTTTTTAATG GACAAAGCGG TCAAATATTA TTCAGAAGCA ATGGGAATAT TACCAGAGAA
CTCACCAATA TCACATTTGG TTTCAGAACA AGGGATGCAA ATGTAATAAT ATTGCATGCA
GAAAAAGAGC CTGAATTTCT TAATATTAGC ATTCAAGATT CCAGATTATT CTTTCAATTG
CAAAGTGGCA ACAGCTTTTA TATGCTAAGT CTGACAAGTT TGCAGTCAGT GAATGATGGC
ACATGGCACG AAGTGACCCT TTCCATGACA GACCCACTGT CCCAGACCTC CAGGTGGCAA
ATGGAAGTGG ACAACGAAAC ACCTTTTGTG ACCAGCACAA TTGCTACTGG AAGCCTCAAC
TTTTTGAAGG ATAATACAGA TATTTATGTG GGAGACAGAG CTATTGACAA TATAAAGGGC
CTGCAAGGGT GTCTAAGTAC AATAGAAATC GGAGGCATTT ATCTCTCTTA CTTTGAAAAT
GTTCATGGTT TCATTAATAA ACCTCAGGAA GAGCAATTTC TCAAAATCTC TACCAATTCA
GTGGTCACTG GCTGTTTGCA GTTAAATGTC TGCAACTCCA ACCCCTGTTT GCATGGAGGA
AACTGTGAAG ACATCTATAG CTCTTATCAT TGCTCCTGTC CCTTGGGATG GTCAGGGAAA
CACTGTGAAC TCAACATCGA TGAATGCTTT TCAAACCCCT GTATCCATGG CAACTGCTCT
GACAGAGTTG CAGCCTACCA CTGCACATGT GAGCCTGGAT ACACTGGTGT GAACTGTGAA
GTGGATATAG ACAACTGCCA GAGTCACCAG TGTGCAAATG GAGCCACCTG CATTAGTCAT
ACTAATGGCT ATTCTTGCCT CTGTTTTGGA AATTTTACAG GAAAATTTTG CAGACAGAGC
AGATTACCCT CAACAGTCTG TGGGAATGAG AAGACAAATC TCACTTGCTA CAATGGAGGC
AACTGCACAG AGTTCCAGAC TGAATTAAAA TGTATGTGCC GGCCAGGTTT TACTGGAGAA
TGGTGTGAAA AGGACATTGA TGAGTGTGCC TCTGATCCGT GTGTCAATGG AGGTCTGTGC
CAGGACTTAC TCAACAAATT CCAGTGCCTC TGTGATGTTG CCTTTGCTGG CGAGCGCTGC
GAGGTGGACT TGGCAGATGA CTTGATCTCC GACATTTTCA CCACTATTGG CTCAGTGACT
GTCGCCTTGT TACTGATCCT CTTGCTGGCC ATTGTTGCTT CTGTTGTCAC CTCCAACAAA
AGGGCAACTC AGGGAACCTA CAGCCCCAGC CGTCAGGAGA AGGAGGGCTC CCGAGTGGAA
ATGTGGAACT TGATGCCACC CCCTGCAATG GAGAGACTGA TTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22677
Clone ID Related Accession (Same CDS sequence) NM_001257965
Accession Version NM_001257965.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product protein crumbs homolog 1 isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302826.1, AK289733.1, AL136322.24 and AY043325.1. Transcript Variant: This variant (3) contains three noncoding exons in place of the first exon and contains an alternate in-frame exon compared to variant 1. The resulting isoform (3) is shorter at the N- terminus and contains an alternate internal segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302826.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968832, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
ATGAAAGACC CTTGCTTCTC CAATCCCTGT CAAGGAAGTG CCACTTGTGT GAACACCCCA 
GGAGAAAGGA GCTTTCTGTG CAAATGTCCT CCTGGGTACA GTGGGACAAT CTGTGAAACT
ACCATTGGTT CCTGTGGCAA GAACTCCTGC CAACATGGAG GTATTTGCCA TCAGGACCCT
ATTTATCCTG TCTGCATCTG CCCTGCTGGA TATGCTGGAA GATTCTGTGA GATAGATCAC
GATGAGTGTG CTTCCAGCCC TTGCCAAAAT GGGGCCGTGT GCCAGGATGG AATTGATGGT
TACTCCTGCT TCTGTGTCCC AGGATATCAA GGCAGACACT GCGACTTGGA AGTGGATGAA
TGTGCTTCAG ATCCCTGCAA GAACGAGGCT ACATGCCTCA ATGAAATAGG AAGATATACT
TGTATCTGTC CCCACAATTA TTCTGGTGTA AACTGTGAAT TGGAAATTGA CGAATGTTGG
TCCCAGCCTT GTTTAAATGG TGCAACTTGT CAGGATGCTC TGGGGGCCTA TTTCTGCGAC
TGTGCCCCTG GATTCCTGGG GGATCACTGT GAACTCAACA CTGATGAGTG TGCCAGTCAA
CCTTGTCTCC ATGGAGGGCT GTGTGTGGAT GGAGAAAACA GATATAGCTG TAACTGCACG
GGTAGTGGAT TCACAGGGAC ACACTGTGAG ACCTTGATGC CTCTTTGTTG GTCAAAACCT
TGTCACAATA ATGCTACATG TGAGGACAGT GTTGACAATT ACACTTGTCA CTGCTGGCCT
GGATACACAG GTGCCCAGTG TGAGATCGAC CTCAATGAAT GCAATAGTAA CCCCTGCCAG
TCCAATGGGG AATGTGTGGA GCTGTCCTCA GAGAAACAAT ATGGACGCAT CACTGGACTG
CCTTCTTCTT TCAGCTACCA TGAAGCCTCA GGTTATGTCT GTATCTGTCA GCCTGGATTC
ACAGGAATCC ACTGCGAAGA AGACGTCAAT GAATGTTCTT CAAACCCTTG CCAAAATGGT
GGTACTTGTG AGAACTTGCC TGGGAATTAT ACTTGCCATT GCCCATTTGA TAACCTTTCT
AGAACTTTTT ATGGAGGAAG GGACTGTTCT GATATTCTCC TGGGCTGTAC CCATCAGCAA
TGTCTAAATA ATGGAACATG CATCCCTCAC TTCCAAGATG GCCAGCATGG ATTCAGCTGC
CTGTGTCCAT CTGGCTACAC CGGGTCCCTG TGTGAAATCG CAACCACACT TTCATTTGAG
GGCGATGGCT TCCTGTGGGT CAAAAGTGGC TCAGTGACAA CCAAGGGCTC AGTTTGTAAC
ATAGCCCTCA GGTTTCAGAC TGTTCAGCCA ATGGCTCTTC TACTTTTCCG AAGCAACAGG
GATGTGTTTG TGAAGCTGGA GCTGCTAAGT GGCTACATTC ACTTATCAAT TCAGGTCAAT
AATCAGTCAA AGGTGCTTCT GTTCATTTCC CACAACACCA GCGATGGAGA GTGGCATTTC
GTGGAGGTAA TATTTGCAGA GGCTGTGACC CTTACCTTAA TCGACGACTC CTGTAAGGAG
AAATGCATCG CGAAAGCTCC TACTCCACTT GAAAGTGATC AATCAATATG TGCTTTTCAG
AACTCCTTTT TGGGTGGTTT ACCAGTGGGA ATGACCAGCA ATGGTGTTGC TCTGCTTAAC
TTCTATAATA TGCCATCCAC ACCTTCGTTT GTAGGCTGTC TCCAAGACAT TAAAATTGAT
TGGAATCACA TTACCCTGGA GAACATCTCG TCTGGCTCAT CATTAAATGT CAAGGCAGGC
TGTGTGAGAA AGGATTGGTG TGAAAGCCAA CCTTGTCAAA GCAGAGGACG CTGCATCAAC
TTGTGGCTGA GTTACCAGTG TGACTGCCAC AGGCCCTATG AAGGCCCCAA CTGTCTGAGA
GAGTATGTGG CAGGCAGATT TGGCCAGGAT GACTCCACTG GTTATGTCAT CTTTACTCTT
GATGAGAGCT ATGGAGACAC CATCAGCCTC TCCATGTTTG TCCGAACGCT TCAACCATCA
GGCTTACTTC TAGCTTTGGA AAACAGCACT TATCAATATA TCCGTGTCTG GCTAGAGCGC
GGCAGACTAG CAATGCTGAC TCCAAACTCT CCCAAATTAG TAGTAAAATT TGTTCTTAAT
GATGGAAATG TCCACTTGAT ATCTTTGAAA ATCAAGCCAT ATAAAATTGA ACTGTATCAG
TCTTCACAAA ACCTAGGATT TATTTCTGCT TCTACGTGGA AAATCGAAAA GGGAGATGTC
ATCTACATTG GTGGCCTACC TGACAAGCAA GAGACTGAAC TTAATGGTGG ATTCTTCAAA
GGCTGTATCC AAGATGTAAG ACTAAACAAC CAAAATCTGG AATTCTTTCC AAATCCAACA
AACAATGCAT CTCTCAATCC AGTTCTTGTC AATGTAACCC AAGGCTGTGC TGGAGACAAC
AGCTGCAAGA GGCAGACCAA TGTGGGAAGG GCACTCACTG AGTTGGGATC CAGAGGACCT
AAGTACCAAG TTTCACTGTT TCGCTTCTGT GTAGGATCTT GGGCAACTGG AAACACCTTC
TTTTTATCAT CTATAAAACC AGGATCCAAC CCCTGTCACA ATGGAGGTGT TTGCCATTCC
CGGTGGGATG ACTTCTCCTG TTCCTGTCCT GCCCTCACAA GTGGGAAAGC CTGTGAGGAG
GTTCAGTGGT GTGGATTCAG CCCGTGTCCT CACGGAGCCC AGTGCCAGCC GGTGCTTCAA
GGATTTGAAT GTATTGCAAA TGCTGTTTTT AATGGACAAA GCGGTCAAAT ATTATTCAGA
AGCAATGGGA ATATTACCAG AGAACTCACC AATATCACAT TTGGTTTCAG AACAAGGGAT
GCAAATGTAA TAATATTGCA TGCAGAAAAA GAGCCTGAAT TTCTTAATAT TAGCATTCAA
GATTCCAGAT TATTCTTTCA ATTGCAAAGT GGCAACAGCT TTTATATGCT AAGTCTGACA
AGTTTGCAGT CAGTGAATGA TGGCACATGG CACGAAGTGA CCCTTTCCAT GACAGACCCA
CTGTCCCAGA CCTCCAGGTG GCAAATGGAA GTGGACAACG AAACACCTTT TGTGACCAGC
ACAATTGCTA CTGGAAGCCT CAACTTTTTG AAGGATAATA CAGATATTTA TGTGGGAGAC
AGAGCTATTG ACAATATAAA GGGCCTGCAA GGGTGTCTAA GTACAATAGA AATCGGAGGC
ATTTATCTCT CTTACTTTGA AAATGTTCAT GGTTTCATTA ATAAACCTCA GGAAGAGCAA
TTTCTCAAAA TCTCTACCAA TTCAGTGGTC ACTGGCTGTT TGCAGTTAAA TGTCTGCAAC
TCCAACCCCT GTTTGCATGG AGGAAACTGT GAAGACATCT ATAGCTCTTA TCATTGCTCC
TGTCCCTTGG GATGGTCAGG GAAACACTGT GAACTCAACA TCGATGAATG CTTTTCAAAC
CCCTGTATCC ATGGCAACTG CTCTGACAGA GTTGCAGCCT ACCACTGCAC ATGTGAGCCT
GGATACACTG GTGTGAACTG TGAAGTGGAT ATAGACAACT GCCAGAGTCA CCAGTGTGCA
AATGGAGCCA CCTGCATTAG TCATACTAAT GGCTATTCTT GCCTCTGTTT TGGAAATTTT
ACAGGAAAAT TTTGCAGACA GAGCAGATTA CCCTCAACAG TCTGTGGGAA TGAGAAGACA
AATCTCACTT GCTACAATGG AGGCAACTGC ACAGAGTTCC AGACTGAATT AAAATGTATG
TGCCGGCCAG GTTTTACTGG AGAATGGTGT GAAAAGGACA TTGATGAGTG TGCCTCTGAT
CCGTGTGTCA ATGGAGGTCT GTGCCAGGAC TTACTCAACA AATTCCAGTG CCTCTGTGAT
GTTGCCTTTG CTGGCGAGCG CTGCGAGGTG GACTTGGCAG ATGACTTGAT CTCCGACATT
TTCACCACTA TTGGCTCAGT GACTGTCGCC TTGTTACTGA TCCTCTTGCT GGCCATTGTT
GCTTCTGTTG TCACCTCCAA CAAAAGGGCA ACTCAGGGAA CCTACAGCCC CAGCCGTCAG
GAGAAGGAGG GCTCCCGAGT GGAAATGTGG AACTTGATGC CACCCCCTGC AATGGAGAGA
CTGATTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001244894.1
CDS340..4488
Misc Feature(1)178..180(+)
Misc Feature(2)346..456(+)
Misc Feature(3)349..393(+)
Misc Feature(4)574..681(+)
Misc Feature(5)574..627(+)
Misc Feature(6)691..798(+)
Misc Feature(7)697..741(+)
Misc Feature(8)805..912(+)
Misc Feature(9)811..855(+)
Misc Feature(10)916..1029(+)
Misc Feature(11)916..969(+)
Misc Feature(12)1057..1143(+)
Misc Feature(13)1321..>1404(+)
Misc Feature(14)1321..1374(+)
Misc Feature(15)1585..2079(+)
Misc Feature(16)2158..2253(+)
Misc Feature(17)2290..2706(+)
Misc Feature(18)2947..3036(+)
Misc Feature(19)3121..3573(+)
Misc Feature(20)3703..3792(+)
Misc Feature(21)3796..3903(+)
Misc Feature(22)3796..3846(+)
Misc Feature(23)3907..4014(+)
Misc Feature(24)3907..3960(+)
Misc Feature(25)4156..4266(+)
Misc Feature(26)4156..4209(+)
Translation

Target ORF information:

RefSeq Version NM_001257965
Organism Homo sapiens (human)
Definition Homo sapiens crumbs family member 1, photoreceptor morphogenesis associated (CRB1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001257965

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
ATGAAAGACC CTTGCTTCTC CAATCCCTGT CAAGGAAGTG CCACTTGTGT GAACACCCCA 
GGAGAAAGGA GCTTTCTGTG CAAATGTCCT CCTGGGTACA GTGGGACAAT CTGTGAAACT
ACCATTGGTT CCTGTGGCAA GAACTCCTGC CAACATGGAG GTATTTGCCA TCAGGACCCT
ATTTATCCTG TCTGCATCTG CCCTGCTGGA TATGCTGGAA GATTCTGTGA GATAGATCAC
GATGAGTGTG CTTCCAGCCC TTGCCAAAAT GGGGCCGTGT GCCAGGATGG AATTGATGGT
TACTCCTGCT TCTGTGTCCC AGGATATCAA GGCAGACACT GCGACTTGGA AGTGGATGAA
TGTGCTTCAG ATCCCTGCAA GAACGAGGCT ACATGCCTCA ATGAAATAGG AAGATATACT
TGTATCTGTC CCCACAATTA TTCTGGTGTA AACTGTGAAT TGGAAATTGA CGAATGTTGG
TCCCAGCCTT GTTTAAATGG TGCAACTTGT CAGGATGCTC TGGGGGCCTA TTTCTGCGAC
TGTGCCCCTG GATTCCTGGG GGATCACTGT GAACTCAACA CTGATGAGTG TGCCAGTCAA
CCTTGTCTCC ATGGAGGGCT GTGTGTGGAT GGAGAAAACA GATATAGCTG TAACTGCACG
GGTAGTGGAT TCACAGGGAC ACACTGTGAG ACCTTGATGC CTCTTTGTTG GTCAAAACCT
TGTCACAATA ATGCTACATG TGAGGACAGT GTTGACAATT ACACTTGTCA CTGCTGGCCT
GGATACACAG GTGCCCAGTG TGAGATCGAC CTCAATGAAT GCAATAGTAA CCCCTGCCAG
TCCAATGGGG AATGTGTGGA GCTGTCCTCA GAGAAACAAT ATGGACGCAT CACTGGACTG
CCTTCTTCTT TCAGCTACCA TGAAGCCTCA GGTTATGTCT GTATCTGTCA GCCTGGATTC
ACAGGAATCC ACTGCGAAGA AGACGTCAAT GAATGTTCTT CAAACCCTTG CCAAAATGGT
GGTACTTGTG AGAACTTGCC TGGGAATTAT ACTTGCCATT GCCCATTTGA TAACCTTTCT
AGAACTTTTT ATGGAGGAAG GGACTGTTCT GATATTCTCC TGGGCTGTAC CCATCAGCAA
TGTCTAAATA ATGGAACATG CATCCCTCAC TTCCAAGATG GCCAGCATGG ATTCAGCTGC
CTGTGTCCAT CTGGCTACAC CGGGTCCCTG TGTGAAATCG CAACCACACT TTCATTTGAG
GGCGATGGCT TCCTGTGGGT CAAAAGTGGC TCAGTGACAA CCAAGGGCTC AGTTTGTAAC
ATAGCCCTCA GGTTTCAGAC TGTTCAGCCA ATGGCTCTTC TACTTTTCCG AAGCAACAGG
GATGTGTTTG TGAAGCTGGA GCTGCTAAGT GGCTACATTC ACTTATCAAT TCAGGTCAAT
AATCAGTCAA AGGTGCTTCT GTTCATTTCC CACAACACCA GCGATGGAGA GTGGCATTTC
GTGGAGGTAA TATTTGCAGA GGCTGTGACC CTTACCTTAA TCGACGACTC CTGTAAGGAG
AAATGCATCG CGAAAGCTCC TACTCCACTT GAAAGTGATC AATCAATATG TGCTTTTCAG
AACTCCTTTT TGGGTGGTTT ACCAGTGGGA ATGACCAGCA ATGGTGTTGC TCTGCTTAAC
TTCTATAATA TGCCATCCAC ACCTTCGTTT GTAGGCTGTC TCCAAGACAT TAAAATTGAT
TGGAATCACA TTACCCTGGA GAACATCTCG TCTGGCTCAT CATTAAATGT CAAGGCAGGC
TGTGTGAGAA AGGATTGGTG TGAAAGCCAA CCTTGTCAAA GCAGAGGACG CTGCATCAAC
TTGTGGCTGA GTTACCAGTG TGACTGCCAC AGGCCCTATG AAGGCCCCAA CTGTCTGAGA
GAGTATGTGG CAGGCAGATT TGGCCAGGAT GACTCCACTG GTTATGTCAT CTTTACTCTT
GATGAGAGCT ATGGAGACAC CATCAGCCTC TCCATGTTTG TCCGAACGCT TCAACCATCA
GGCTTACTTC TAGCTTTGGA AAACAGCACT TATCAATATA TCCGTGTCTG GCTAGAGCGC
GGCAGACTAG CAATGCTGAC TCCAAACTCT CCCAAATTAG TAGTAAAATT TGTTCTTAAT
GATGGAAATG TCCACTTGAT ATCTTTGAAA ATCAAGCCAT ATAAAATTGA ACTGTATCAG
TCTTCACAAA ACCTAGGATT TATTTCTGCT TCTACGTGGA AAATCGAAAA GGGAGATGTC
ATCTACATTG GTGGCCTACC TGACAAGCAA GAGACTGAAC TTAATGGTGG ATTCTTCAAA
GGCTGTATCC AAGATGTAAG ACTAAACAAC CAAAATCTGG AATTCTTTCC AAATCCAACA
AACAATGCAT CTCTCAATCC AGTTCTTGTC AATGTAACCC AAGGCTGTGC TGGAGACAAC
AGCTGCAAGA GGCAGACCAA TGTGGGAAGG GCACTCACTG AGTTGGGATC CAGAGGACCT
AAGTACCAAG TTTCACTGTT TCGCTTCTGT GTAGGATCTT GGGCAACTGG AAACACCTTC
TTTTTATCAT CTATAAAACC AGGATCCAAC CCCTGTCACA ATGGAGGTGT TTGCCATTCC
CGGTGGGATG ACTTCTCCTG TTCCTGTCCT GCCCTCACAA GTGGGAAAGC CTGTGAGGAG
GTTCAGTGGT GTGGATTCAG CCCGTGTCCT CACGGAGCCC AGTGCCAGCC GGTGCTTCAA
GGATTTGAAT GTATTGCAAA TGCTGTTTTT AATGGACAAA GCGGTCAAAT ATTATTCAGA
AGCAATGGGA ATATTACCAG AGAACTCACC AATATCACAT TTGGTTTCAG AACAAGGGAT
GCAAATGTAA TAATATTGCA TGCAGAAAAA GAGCCTGAAT TTCTTAATAT TAGCATTCAA
GATTCCAGAT TATTCTTTCA ATTGCAAAGT GGCAACAGCT TTTATATGCT AAGTCTGACA
AGTTTGCAGT CAGTGAATGA TGGCACATGG CACGAAGTGA CCCTTTCCAT GACAGACCCA
CTGTCCCAGA CCTCCAGGTG GCAAATGGAA GTGGACAACG AAACACCTTT TGTGACCAGC
ACAATTGCTA CTGGAAGCCT CAACTTTTTG AAGGATAATA CAGATATTTA TGTGGGAGAC
AGAGCTATTG ACAATATAAA GGGCCTGCAA GGGTGTCTAA GTACAATAGA AATCGGAGGC
ATTTATCTCT CTTACTTTGA AAATGTTCAT GGTTTCATTA ATAAACCTCA GGAAGAGCAA
TTTCTCAAAA TCTCTACCAA TTCAGTGGTC ACTGGCTGTT TGCAGTTAAA TGTCTGCAAC
TCCAACCCCT GTTTGCATGG AGGAAACTGT GAAGACATCT ATAGCTCTTA TCATTGCTCC
TGTCCCTTGG GATGGTCAGG GAAACACTGT GAACTCAACA TCGATGAATG CTTTTCAAAC
CCCTGTATCC ATGGCAACTG CTCTGACAGA GTTGCAGCCT ACCACTGCAC ATGTGAGCCT
GGATACACTG GTGTGAACTG TGAAGTGGAT ATAGACAACT GCCAGAGTCA CCAGTGTGCA
AATGGAGCCA CCTGCATTAG TCATACTAAT GGCTATTCTT GCCTCTGTTT TGGAAATTTT
ACAGGAAAAT TTTGCAGACA GAGCAGATTA CCCTCAACAG TCTGTGGGAA TGAGAAGACA
AATCTCACTT GCTACAATGG AGGCAACTGC ACAGAGTTCC AGACTGAATT AAAATGTATG
TGCCGGCCAG GTTTTACTGG AGAATGGTGT GAAAAGGACA TTGATGAGTG TGCCTCTGAT
CCGTGTGTCA ATGGAGGTCT GTGCCAGGAC TTACTCAACA AATTCCAGTG CCTCTGTGAT
GTTGCCTTTG CTGGCGAGCG CTGCGAGGTG GACTTGGCAG ATGACTTGAT CTCCGACATT
TTCACCACTA TTGGCTCAGT GACTGTCGCC TTGTTACTGA TCCTCTTGCT GGCCATTGTT
GCTTCTGTTG TCACCTCCAA CAAAAGGGCA ACTCAGGGAA CCTACAGCCC CAGCCGTCAG
GAGAAGGAGG GCTCCCGAGT GGAAATGTGG AACTTGATGC CACCCCCTGC AATGGAGAGA
CTGATTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22808
Clone ID Related Accession (Same CDS sequence) NM_001257966
Accession Version NM_001257966.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2613bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product protein crumbs homolog 1 isoform 4 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC370545.1, AL513325.13, AK299368.1 and AY043325.1. Transcript Variant: This variant (4) lacks an alternate in-frame segment of two coding exons and most of a third compared to variant 1. The resulting isoform (4) has the same N- and C-termini but lacks an alternate internal segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK299368.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC
TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG
GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA
CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA
GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC
TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT
GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC
AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG
AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT
TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA
TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT
TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT
ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC
CAAGATGGCC AGCATGGATT CAGCTGCCTG TGTCCATCTG GCTACACCGG GTCCCTGTGT
GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA
GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG
GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC
TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC
AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT
ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA
AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG
ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA
GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT
GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT
TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG
CCCTATGAAG GCCCCAACTG TCTGAGAGGA AAATTTTGCA GACAGAGCAG ATTACCCTCA
ACAGTCTGTG GGAATGAGAA GACAAATCTC ACTTGCTACA ATGGAGGCAA CTGCACAGAG
TTCCAGACTG AATTAAAATG TATGTGCCGG CCAGGTTTTA CTGGAGAATG GTGTGAAAAG
GACATTGATG AGTGTGCCTC TGATCCGTGT GTCAATGGAG GTCTGTGCCA GGACTTACTC
AACAAATTCC AGTGCCTCTG TGATGTTGCC TTTGCTGGCG AGCGCTGCGA GGTGGACTTG
GCAGATGACT TGATCTCCGA CATTTTCACC ACTATTGGCT CAGTGACTGT CGCCTTGTTA
CTGATCCTCT TGCTGGCCAT TGTTGCTTCT GTTGTCACCT CCAACAAAAG GGCAACTCAG
GGAACCTACA GCCCCAGCCG TCAGGAGAAG GAGGGCTCCC GAGTGGAAAT GTGGAACTTG
ATGCCACCCC CTGCAATGGA GAGACTGATT TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001244895.1
CDS210..2822
Misc Feature(1)132..134(+)
Misc Feature(2)423..533(+)
Misc Feature(3)426..470(+)
Misc Feature(4)651..758(+)
Misc Feature(5)651..704(+)
Misc Feature(6)768..875(+)
Misc Feature(7)774..818(+)
Misc Feature(8)882..989(+)
Misc Feature(9)888..932(+)
Misc Feature(10)993..1106(+)
Misc Feature(11)993..1046(+)
Misc Feature(12)1134..1220(+)
Misc Feature(13)1398..>1481(+)
Misc Feature(14)1398..1451(+)
Misc Feature(15)1662..2156(+)
Misc Feature(16)2238..2330(+)
Misc Feature(17)2490..2600(+)
Misc Feature(18)2490..2543(+)
Exon (1)1..279
Gene:CRB1
Gene Synonym:
Exon (2)280..861
Gene:CRB1
Gene Synonym:
Exon (3)862..1057
Gene:CRB1
Gene Synonym:
Exon (4)1058..1197
Gene:CRB1
Gene Synonym:
Exon (5)1198..1380
Gene:CRB1
Gene Synonym:
Exon (6)1381..2337
Gene:CRB1
Gene Synonym:
Exon (7)2338..2350
Gene:CRB1
Gene Synonym:
Exon (8)2351..2479
Gene:CRB1
Gene Synonym:
Exon (9)2480..2606
Gene:CRB1
Gene Synonym:
Exon (10)2607..3398
Gene:CRB1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001257966
Organism Homo sapiens (human)
Definition Homo sapiens crumbs family member 1, photoreceptor morphogenesis associated (CRB1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001257966

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGGCACTTA AGAACATTAA CTACCTTCTC ATCTTCTACC TCAGTTTCTC ACTGCTTATC 
TACATAAAAA ATTCCTTTTG CAATAAAAAC AACACCAGGT GCCTCTCAAA TTCTTGCCAA
AACAATTCTA CATGCAAAGA TTTTTCAAAA GACAATGATT GTTCTTGTTC AGACACAGCC
AATAATTTGG ACAAAGACTG TGACAACATG AAAGACCCTT GCTTCTCCAA TCCCTGTCAA
GGAAGTGCCA CTTGTGTGAA CACCCCAGGA GAAAGGAGCT TTCTGTGCAA ATGTCCTCCT
GGGTACAGTG GGACAATCTG TGAAACTACC ATTGGTTCCT GTGGCAAGAA CTCCTGCCAA
CATGGAGGTA TTTGCCATCA GGACCCTATT TATCCTGTCT GCATCTGCCC TGCTGGATAT
GCTGGAAGAT TCTGTGAGAT AGATCACGAT GAGTGTGCTT CCAGCCCTTG CCAAAATGGG
GCCGTGTGCC AGGATGGAAT TGATGGTTAC TCCTGCTTCT GTGTCCCAGG ATATCAAGGC
AGACACTGCG ACTTGGAAGT GGATGAATGT GCTTCAGATC CCTGCAAGAA CGAGGCTACA
TGCCTCAATG AAATAGGAAG ATATACTTGT ATCTGTCCCC ACAATTATTC TGGTGTAAAC
TGTGAATTGG AAATTGACGA ATGTTGGTCC CAGCCTTGTT TAAATGGTGC AACTTGTCAG
GATGCTCTGG GGGCCTATTT CTGCGACTGT GCCCCTGGAT TCCTGGGGGA TCACTGTGAA
CTCAACACTG ATGAGTGTGC CAGTCAACCT TGTCTCCATG GAGGGCTGTG TGTGGATGGA
GAAAACAGAT ATAGCTGTAA CTGCACGGGT AGTGGATTCA CAGGGACACA CTGTGAGACC
TTGATGCCTC TTTGTTGGTC AAAACCTTGT CACAATAATG CTACATGTGA GGACAGTGTT
GACAATTACA CTTGTCACTG CTGGCCTGGA TACACAGGTG CCCAGTGTGA GATCGACCTC
AATGAATGCA ATAGTAACCC CTGCCAGTCC AATGGGGAAT GTGTGGAGCT GTCCTCAGAG
AAACAATATG GACGCATCAC TGGACTGCCT TCTTCTTTCA GCTACCATGA AGCCTCAGGT
TATGTCTGTA TCTGTCAGCC TGGATTCACA GGAATCCACT GCGAAGAAGA CGTCAATGAA
TGTTCTTCAA ACCCTTGCCA AAATGGTGGT ACTTGTGAGA ACTTGCCTGG GAATTATACT
TGCCATTGCC CATTTGATAA CCTTTCTAGA ACTTTTTATG GAGGAAGGGA CTGTTCTGAT
ATTCTCCTGG GCTGTACCCA TCAGCAATGT CTAAATAATG GAACATGCAT CCCTCACTTC
CAAGATGGCC AGCATGGATT CAGCTGCCTG TGTCCATCTG GCTACACCGG GTCCCTGTGT
GAAATCGCAA CCACACTTTC ATTTGAGGGC GATGGCTTCC TGTGGGTCAA AAGTGGCTCA
GTGACAACCA AGGGCTCAGT TTGTAACATA GCCCTCAGGT TTCAGACTGT TCAGCCAATG
GCTCTTCTAC TTTTCCGAAG CAACAGGGAT GTGTTTGTGA AGCTGGAGCT GCTAAGTGGC
TACATTCACT TATCAATTCA GGTCAATAAT CAGTCAAAGG TGCTTCTGTT CATTTCCCAC
AACACCAGCG ATGGAGAGTG GCATTTCGTG GAGGTAATAT TTGCAGAGGC TGTGACCCTT
ACCTTAATCG ACGACTCCTG TAAGGAGAAA TGCATCGCGA AAGCTCCTAC TCCACTTGAA
AGTGATCAAT CAATATGTGC TTTTCAGAAC TCCTTTTTGG GTGGTTTACC AGTGGGAATG
ACCAGCAATG GTGTTGCTCT GCTTAACTTC TATAATATGC CATCCACACC TTCGTTTGTA
GGCTGTCTCC AAGACATTAA AATTGATTGG AATCACATTA CCCTGGAGAA CATCTCGTCT
GGCTCATCAT TAAATGTCAA GGCAGGCTGT GTGAGAAAGG ATTGGTGTGA AAGCCAACCT
TGTCAAAGCA GAGGACGCTG CATCAACTTG TGGCTGAGTT ACCAGTGTGA CTGCCACAGG
CCCTATGAAG GCCCCAACTG TCTGAGAGGA AAATTTTGCA GACAGAGCAG ATTACCCTCA
ACAGTCTGTG GGAATGAGAA GACAAATCTC ACTTGCTACA ATGGAGGCAA CTGCACAGAG
TTCCAGACTG AATTAAAATG TATGTGCCGG CCAGGTTTTA CTGGAGAATG GTGTGAAAAG
GACATTGATG AGTGTGCCTC TGATCCGTGT GTCAATGGAG GTCTGTGCCA GGACTTACTC
AACAAATTCC AGTGCCTCTG TGATGTTGCC TTTGCTGGCG AGCGCTGCGA GGTGGACTTG
GCAGATGACT TGATCTCCGA CATTTTCACC ACTATTGGCT CAGTGACTGT CGCCTTGTTA
CTGATCCTCT TGCTGGCCAT TGTTGCTTCT GTTGTCACCT CCAACAAAAG GGCAACTCAG
GGAACCTACA GCCCCAGCCG TCAGGAGAAG GAGGGCTCCC GAGTGGAAAT GTGGAACTTG
ATGCCACCCC CTGCAATGGA GAGACTGATT TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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