ORF cDNA Clones and Custom Clones » Species Summary » Homo sapiens » BRD4 cDNA ORF clone

BRD4 cDNA ORF clone, Homo sapiens(human)

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Summary

Gene Symbol	BRD4
Entrez Gene ID	23476
Full Name	bromodomain containing 4
Synonyms	CAP,HUNK1,HUNKI,MCAP
General protein information	Preferred Names bromodomain containing 4 Names bromodomain-containing protein 4 chromosome-associated protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.12
Summary	The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008].
Disorder MIM:	608749

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011527856.1	XP_011526158.1	bromodomain-containing protein 4 isoform X2
XM_011527854.1	XP_011526156.1	bromodomain-containing protein 4 isoform X1
NM_001330384.1	NP_001317313.1	bromodomain-containing protein 4 isoform 3
XM_011527856.2	XP_011526158.1	bromodomain-containing protein 4 isoform X3
XM_011527854.2	XP_011526156.1	bromodomain-containing protein 4 isoform X1
XM_011527856.3	XP_011526158.1	bromodomain-containing protein 4 isoform X2
NM_014299.2	NP_055114.1	bromodomain-containing protein 4 isoform short
NM_058243.2	NP_490597.1	bromodomain-containing protein 4 isoform long
NM_001330384.2	NP_001317313.1	bromodomain-containing protein 4 isoform 3

Homologies

Gallus gallus (chicken)	LOC100859056	XP_003643891.2
Pan troglodytes (chimpanzee)	BRD4	XP_512452.4
Homo sapiens (human)	BRD4	NP_490597.1

The following BRD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26888	NM_014299.2 Latest version!	Homo sapiens bromodomain containing 4 (BRD4), transcript variant short, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	in pcDNA3.1⁺/C-(K)DYK	$279.50-$391.30 $559.00
OHu26888	XM_011527856.3 Latest version!	Homo sapiens bromodomain containing 4 (BRD4), transcript variant X2, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	in pcDNA3.1⁺/C-(K)DYK	$279.50-$391.30 $559.00
OHu66214	NM_001330384.2 Latest version!	Homo sapiens bromodomain containing 4 (BRD4), transcript variant 3, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	12-14	$391.30 $559.00
OHu27190	NM_058243.2 Latest version!	Homo sapiens bromodomain containing 4 (BRD4), transcript variant long, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	TBD	$923.30 $1319.00
OHu27190	XM_011527854.2 Latest version!	Homo sapiens bromodomain containing 4 (BRD4), transcript variant X1, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	TBD	$923.30 $1319.00
View Old Accession Versions >>
OHu26888	XM_011527856.1	Homo sapiens bromodomain containing 4 (BRD4), transcript variant X3, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	in pcDNA3.1⁺/C-(K)DYK	$279.50-$391.30 $559.00
OHu27190	XM_011527854.1	Homo sapiens bromodomain containing 4 (BRD4), transcript variant X1, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	TBD	$923.30 $1319.00
OHu26888	XM_011527856.2	Homo sapiens bromodomain containing 4 (BRD4), transcript variant X3, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	in pcDNA3.1⁺/C-(K)DYK	$279.50-$391.30 $559.00
OHu66214	NM_001330384.1	Homo sapiens bromodomain containing 4 (BRD4), transcript variant 3, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRD4 ( NM_014299.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_055114.1 Homo sapiens bromodomain containing 4 (BRD4), transcript variant short, mRNA.

Price & Availability↑

CloneID	OHu26888
Clone ID Related Accession (Same CDS sequence)	XM_011527856.1 , XM_011527856.2 , XM_011527856.3 , NM_014299.2
Accession Version	NM_014299.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2169bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Homo sapiens(human)
Product	bromodomain-containing protein 4 isoform short
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Y12059.1, BC035266.1 and AC004798.1. On Aug 18, 2006 this sequence version replaced NM_014299.1. Transcript Variant: This variant (short) differs in the 3' UTR and coding region compared to variant long. The resulting isoform (short) is shorter and has a distinct C-terminus compared to isoform long. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y12059.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCGG AGAGCGGCCC TGGGACGAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACTT CCCAAATGTC TACAACACAG GCCCAGGCCC AACCCCAGCC AGCCAACGCA 
GCCAGCACCA ACCCCCCGCC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATACCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCA 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATC 
ATTAAAACGC CTATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTACAAC 
AAGCCTGGAG ATGACATAGT CTTAATGGCA GAAGCTCTGG AAAAGCTCTT CTTGCAAAAA 
ATAAATGAGC TACCCACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGTGGGAGGA AAGAAACAGG GACAGCAAAA CCTGGCGTTT CCACGGTACC AAACACAACT 
CAAGCATCGA CTCCTCCGCA GACCCAGACC CCTCAGCCGA ATCCTCCTCC TGTGCAGGCC 
ACGCCTCACC CCTTCCCTGC CGTCACCCCG GACCTCATCG TCCAGACCCC TGTCATGACA 
GTGGTGCCTC CCCAGCCACT GCAGACGCCC CCGCCAGTGC CCCCCCAGCC ACAACCCCCA 
CCCGCTCCAG CTCCCCAGCC CGTACAGAGC CACCCACCCA TCATCGCGGC CACCCCACAG 
CCTGTGAAGA CAAAGAAGGG AGTGAAGAGG AAAGCAGACA CCACCACCCC CACCACCATT 
GACCCCATTC ACGAGCCACC CTCGCTGCCC CCGGAGCCCA AGACCACCAA GCTGGGCCAG 
CGGCGGGAGA GCAGCCGGCC TGTGAAACCT CCAAAGAAGG ACGTGCCCGA CTCTCAGCAG 
CACCCAGCAC CAGAGAAGAG CAGCAAGGTC TCGGAGCAGC TCAAGTGCTG CAGCGGCATC 
CTCAAGGAGA TGTTTGCCAA GAAGCACGCC GCCTACGCCT GGCCCTTCTA CAAGCCTGTG 
GACGTGGAGG CACTGGGCCT ACACGACTAC TGTGACATCA TCAAGCACCC CATGGACATG 
AGCACAATCA AGTCTAAACT GGAGGCCCGT GAGTACCGTG ATGCTCAGGA GTTTGGTGCT 
GACGTCCGAT TGATGTTCTC CAACTGCTAT AAGTACAACC CTCCTGACCA TGAGGTGGTG 
GCCATGGCCC GCAAGCTCCA GGATGTGTTC GAAATGCGCT TTGCCAAGAT GCCGGACGAG 
CCTGAGGAGC CAGTGGTGGC CGTGTCCTCC CCGGCAGTGC CCCCTCCCAC CAAGGTTGTG 
GCCCCGCCCT CATCCAGCGA CAGCAGCAGC GATAGCTCCT CGGACAGTGA CAGTTCGACT 
GATGACTCTG AGGAGGAGCG AGCCCAGCGG CTGGCTGAGC TCCAGGAGCA GCTCAAAGCC 
GTGCACGAGC AGCTTGCAGC CCTCTCTCAG CCCCAGCAGA ACAAACCAAA GAAAAAGGAG 
AAAGACAAGA AGGAAAAGAA AAAAGAAAAG CACAAAAGGA AAGAGGAAGT GGAAGAGAAT 
AAAAAAAGCA AAGCCAAGGA ACCTCCTCCT AAAAAGACGA AGAAAAATAA TAGCAGCAAC 
AGCAATGTGA GCAAGAAGGA GCCAGCGCCC ATGAAGAGCA AGCCCCCTCC CACGTATGAG 
TCGGAGGAAG AGGACAAGTG CAAGCCTATG TCCTATGAGG AGAAGCGGCA GCTCAGCTTG 
GACATCAACA AGCTCCCCGG CGAGAAGCTG GGCCGCGTGG TGCACATCAT CCAGTCACGG 
GAGCCCTCCC TGAAGAATTC CAACCCCGAC GAGATTGAAA TCGACTTTGA GACCCTGAAG 
CCGTCCACAC TGCGTGAGCT GGAGCGCTAT GTCACCTCCT GTTTGCGGAA GAAAAGGAAA 
CCTCAAGCTG AGAAAGTTGA TGTGATTGCC GGCTCCTCCA AGATGAAGGG CTTCTCGTCC 
TCAGAGTCGG AGAGCTCCAG TGAGTCCAGC TCCTCTGACA GCGAAGACTC CGAAACAGGT 
CCTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055114.1
CDS	223..2391
Translation	MSAESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGTAKPGVSTVPNTTQASTPPQTQTPQPNPPPVQATPHPFPAVTPDLIVQTPVMTVVPPQPLQTPPPVPPQPQPPPAPAPQPVQSHPPIIAATPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLPPEPKTTKLGQRRESSRPVKPPKKDVPDSQQHPAPEKSSKVSEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLEAREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVAVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKRKEEVEENKKSKAKEPPPKKTKKNNSSNSNVSKKEPAPMKSKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESSSESSSSDSEDSETGPA

Target ORF information:

RefSeq Version	NM_014299.2
Organism	Homo sapiens(human)
Definition	Homo sapiens bromodomain containing 4 (BRD4), transcript variant short, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014299.2

ORF Insert Sequence:

ATGTCTGCGG AGAGCGGCCC TGGGACGAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACTT CCCAAATGTC TACAACACAG GCCCAGGCCC AACCCCAGCC AGCCAACGCA 
GCCAGCACCA ACCCCCCGCC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATACCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCA 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATC 
ATTAAAACGC CTATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTACAAC 
AAGCCTGGAG ATGACATAGT CTTAATGGCA GAAGCTCTGG AAAAGCTCTT CTTGCAAAAA 
ATAAATGAGC TACCCACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGTGGGAGGA AAGAAACAGG GACAGCAAAA CCTGGCGTTT CCACGGTACC AAACACAACT 
CAAGCATCGA CTCCTCCGCA GACCCAGACC CCTCAGCCGA ATCCTCCTCC TGTGCAGGCC 
ACGCCTCACC CCTTCCCTGC CGTCACCCCG GACCTCATCG TCCAGACCCC TGTCATGACA 
GTGGTGCCTC CCCAGCCACT GCAGACGCCC CCGCCAGTGC CCCCCCAGCC ACAACCCCCA 
CCCGCTCCAG CTCCCCAGCC CGTACAGAGC CACCCACCCA TCATCGCGGC CACCCCACAG 
CCTGTGAAGA CAAAGAAGGG AGTGAAGAGG AAAGCAGACA CCACCACCCC CACCACCATT 
GACCCCATTC ACGAGCCACC CTCGCTGCCC CCGGAGCCCA AGACCACCAA GCTGGGCCAG 
CGGCGGGAGA GCAGCCGGCC TGTGAAACCT CCAAAGAAGG ACGTGCCCGA CTCTCAGCAG 
CACCCAGCAC CAGAGAAGAG CAGCAAGGTC TCGGAGCAGC TCAAGTGCTG CAGCGGCATC 
CTCAAGGAGA TGTTTGCCAA GAAGCACGCC GCCTACGCCT GGCCCTTCTA CAAGCCTGTG 
GACGTGGAGG CACTGGGCCT ACACGACTAC TGTGACATCA TCAAGCACCC CATGGACATG 
AGCACAATCA AGTCTAAACT GGAGGCCCGT GAGTACCGTG ATGCTCAGGA GTTTGGTGCT 
GACGTCCGAT TGATGTTCTC CAACTGCTAT AAGTACAACC CTCCTGACCA TGAGGTGGTG 
GCCATGGCCC GCAAGCTCCA GGATGTGTTC GAAATGCGCT TTGCCAAGAT GCCGGACGAG 
CCTGAGGAGC CAGTGGTGGC CGTGTCCTCC CCGGCAGTGC CCCCTCCCAC CAAGGTTGTG 
GCCCCGCCCT CATCCAGCGA CAGCAGCAGC GATAGCTCCT CGGACAGTGA CAGTTCGACT 
GATGACTCTG AGGAGGAGCG AGCCCAGCGG CTGGCTGAGC TCCAGGAGCA GCTCAAAGCC 
GTGCACGAGC AGCTTGCAGC CCTCTCTCAG CCCCAGCAGA ACAAACCAAA GAAAAAGGAG 
AAAGACAAGA AGGAAAAGAA AAAAGAAAAG CACAAAAGGA AAGAGGAAGT GGAAGAGAAT 
AAAAAAAGCA AAGCCAAGGA ACCTCCTCCT AAAAAGACGA AGAAAAATAA TAGCAGCAAC 
AGCAATGTGA GCAAGAAGGA GCCAGCGCCC ATGAAGAGCA AGCCCCCTCC CACGTATGAG 
TCGGAGGAAG AGGACAAGTG CAAGCCTATG TCCTATGAGG AGAAGCGGCA GCTCAGCTTG 
GACATCAACA AGCTCCCCGG CGAGAAGCTG GGCCGCGTGG TGCACATCAT CCAGTCACGG 
GAGCCCTCCC TGAAGAATTC CAACCCCGAC GAGATTGAAA TCGACTTTGA GACCCTGAAG 
CCGTCCACAC TGCGTGAGCT GGAGCGCTAT GTCACCTCCT GTTTGCGGAA GAAAAGGAAA 
CCTCAAGCTG AGAAAGTTGA TGTGATTGCC GGCTCCTCCA AGATGAAGGG CTTCTCGTCC 
TCAGAGTCGG AGAGCTCCAG TGAGTCCAGC TCCTCTGACA GCGAAGACTC CGAAACAGGT 
CCTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRD4 ( XM_011527856.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526158.1 Homo sapiens bromodomain containing 4 (BRD4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu26888
Clone ID Related Accession (Same CDS sequence)	XM_011527856.1 , XM_011527856.2 , XM_011527856.3 , NM_014299.2
Accession Version	XM_011527856.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2169bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	bromodomain-containing protein 4 isoform X2
Comment

ATGTCTGCGG AGAGCGGCCC TGGGACGAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACTT CCCAAATGTC TACAACACAG GCCCAGGCCC AACCCCAGCC AGCCAACGCA 
GCCAGCACCA ACCCCCCGCC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATACCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCA 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATC 
ATTAAAACGC CTATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTACAAC 
AAGCCTGGAG ATGACATAGT CTTAATGGCA GAAGCTCTGG AAAAGCTCTT CTTGCAAAAA 
ATAAATGAGC TACCCACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGTGGGAGGA AAGAAACAGG GACAGCAAAA CCTGGCGTTT CCACGGTACC AAACACAACT 
CAAGCATCGA CTCCTCCGCA GACCCAGACC CCTCAGCCGA ATCCTCCTCC TGTGCAGGCC 
ACGCCTCACC CCTTCCCTGC CGTCACCCCG GACCTCATCG TCCAGACCCC TGTCATGACA 
GTGGTGCCTC CCCAGCCACT GCAGACGCCC CCGCCAGTGC CCCCCCAGCC ACAACCCCCA 
CCCGCTCCAG CTCCCCAGCC CGTACAGAGC CACCCACCCA TCATCGCGGC CACCCCACAG 
CCTGTGAAGA CAAAGAAGGG AGTGAAGAGG AAAGCAGACA CCACCACCCC CACCACCATT 
GACCCCATTC ACGAGCCACC CTCGCTGCCC CCGGAGCCCA AGACCACCAA GCTGGGCCAG 
CGGCGGGAGA GCAGCCGGCC TGTGAAACCT CCAAAGAAGG ACGTGCCCGA CTCTCAGCAG 
CACCCAGCAC CAGAGAAGAG CAGCAAGGTC TCGGAGCAGC TCAAGTGCTG CAGCGGCATC 
CTCAAGGAGA TGTTTGCCAA GAAGCACGCC GCCTACGCCT GGCCCTTCTA CAAGCCTGTG 
GACGTGGAGG CACTGGGCCT ACACGACTAC TGTGACATCA TCAAGCACCC CATGGACATG 
AGCACAATCA AGTCTAAACT GGAGGCCCGT GAGTACCGTG ATGCTCAGGA GTTTGGTGCT 
GACGTCCGAT TGATGTTCTC CAACTGCTAT AAGTACAACC CTCCTGACCA TGAGGTGGTG 
GCCATGGCCC GCAAGCTCCA GGATGTGTTC GAAATGCGCT TTGCCAAGAT GCCGGACGAG 
CCTGAGGAGC CAGTGGTGGC CGTGTCCTCC CCGGCAGTGC CCCCTCCCAC CAAGGTTGTG 
GCCCCGCCCT CATCCAGCGA CAGCAGCAGC GATAGCTCCT CGGACAGTGA CAGTTCGACT 
GATGACTCTG AGGAGGAGCG AGCCCAGCGG CTGGCTGAGC TCCAGGAGCA GCTCAAAGCC 
GTGCACGAGC AGCTTGCAGC CCTCTCTCAG CCCCAGCAGA ACAAACCAAA GAAAAAGGAG 
AAAGACAAGA AGGAAAAGAA AAAAGAAAAG CACAAAAGGA AAGAGGAAGT GGAAGAGAAT 
AAAAAAAGCA AAGCCAAGGA ACCTCCTCCT AAAAAGACGA AGAAAAATAA TAGCAGCAAC 
AGCAATGTGA GCAAGAAGGA GCCAGCGCCC ATGAAGAGCA AGCCCCCTCC CACGTATGAG 
TCGGAGGAAG AGGACAAGTG CAAGCCTATG TCCTATGAGG AGAAGCGGCA GCTCAGCTTG 
GACATCAACA AGCTCCCCGG CGAGAAGCTG GGCCGCGTGG TGCACATCAT CCAGTCACGG 
GAGCCCTCCC TGAAGAATTC CAACCCCGAC GAGATTGAAA TCGACTTTGA GACCCTGAAG 
CCGTCCACAC TGCGTGAGCT GGAGCGCTAT GTCACCTCCT GTTTGCGGAA GAAAAGGAAA 
CCTCAAGCTG AGAAAGTTGA TGTGATTGCC GGCTCCTCCA AGATGAAGGG CTTCTCGTCC 
TCAGAGTCGG AGAGCTCCAG TGAGTCCAGC TCCTCTGACA GCGAAGACTC CGAAACAGGT 
CCTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011526158.1

Translation

MSAESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGTAKPGVSTVPNTTQASTPPQTQTPQPNPPPVQATPHPFPAVTPDLIVQTPVMTVVPPQPLQTPPPVPPQPQPPPAPAPQPVQSHPPIIAATPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLPPEPKTTKLGQRRESSRPVKPPKKDVPDSQQHPAPEKSSKVSEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLEAREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVAVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKRKEEVEENKKSKAKEPPPKKTKKNNSSNSNVSKKEPAPMKSKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESSSESSSSDSEDSETGPA

Target ORF information:

RefSeq Version	XM_011527856.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens bromodomain containing 4 (BRD4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527856.1

ORF Insert Sequence:

ATGTCTGCGG AGAGCGGCCC TGGGACGAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACTT CCCAAATGTC TACAACACAG GCCCAGGCCC AACCCCAGCC AGCCAACGCA 
GCCAGCACCA ACCCCCCGCC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATACCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCA 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATC 
ATTAAAACGC CTATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTACAAC 
AAGCCTGGAG ATGACATAGT CTTAATGGCA GAAGCTCTGG AAAAGCTCTT CTTGCAAAAA 
ATAAATGAGC TACCCACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGTGGGAGGA AAGAAACAGG GACAGCAAAA CCTGGCGTTT CCACGGTACC AAACACAACT 
CAAGCATCGA CTCCTCCGCA GACCCAGACC CCTCAGCCGA ATCCTCCTCC TGTGCAGGCC 
ACGCCTCACC CCTTCCCTGC CGTCACCCCG GACCTCATCG TCCAGACCCC TGTCATGACA 
GTGGTGCCTC CCCAGCCACT GCAGACGCCC CCGCCAGTGC CCCCCCAGCC ACAACCCCCA 
CCCGCTCCAG CTCCCCAGCC CGTACAGAGC CACCCACCCA TCATCGCGGC CACCCCACAG 
CCTGTGAAGA CAAAGAAGGG AGTGAAGAGG AAAGCAGACA CCACCACCCC CACCACCATT 
GACCCCATTC ACGAGCCACC CTCGCTGCCC CCGGAGCCCA AGACCACCAA GCTGGGCCAG 
CGGCGGGAGA GCAGCCGGCC TGTGAAACCT CCAAAGAAGG ACGTGCCCGA CTCTCAGCAG 
CACCCAGCAC CAGAGAAGAG CAGCAAGGTC TCGGAGCAGC TCAAGTGCTG CAGCGGCATC 
CTCAAGGAGA TGTTTGCCAA GAAGCACGCC GCCTACGCCT GGCCCTTCTA CAAGCCTGTG 
GACGTGGAGG CACTGGGCCT ACACGACTAC TGTGACATCA TCAAGCACCC CATGGACATG 
AGCACAATCA AGTCTAAACT GGAGGCCCGT GAGTACCGTG ATGCTCAGGA GTTTGGTGCT 
GACGTCCGAT TGATGTTCTC CAACTGCTAT AAGTACAACC CTCCTGACCA TGAGGTGGTG 
GCCATGGCCC GCAAGCTCCA GGATGTGTTC GAAATGCGCT TTGCCAAGAT GCCGGACGAG 
CCTGAGGAGC CAGTGGTGGC CGTGTCCTCC CCGGCAGTGC CCCCTCCCAC CAAGGTTGTG 
GCCCCGCCCT CATCCAGCGA CAGCAGCAGC GATAGCTCCT CGGACAGTGA CAGTTCGACT 
GATGACTCTG AGGAGGAGCG AGCCCAGCGG CTGGCTGAGC TCCAGGAGCA GCTCAAAGCC 
GTGCACGAGC AGCTTGCAGC CCTCTCTCAG CCCCAGCAGA ACAAACCAAA GAAAAAGGAG 
AAAGACAAGA AGGAAAAGAA AAAAGAAAAG CACAAAAGGA AAGAGGAAGT GGAAGAGAAT 
AAAAAAAGCA AAGCCAAGGA ACCTCCTCCT AAAAAGACGA AGAAAAATAA TAGCAGCAAC 
AGCAATGTGA GCAAGAAGGA GCCAGCGCCC ATGAAGAGCA AGCCCCCTCC CACGTATGAG 
TCGGAGGAAG AGGACAAGTG CAAGCCTATG TCCTATGAGG AGAAGCGGCA GCTCAGCTTG 
GACATCAACA AGCTCCCCGG CGAGAAGCTG GGCCGCGTGG TGCACATCAT CCAGTCACGG 
GAGCCCTCCC TGAAGAATTC CAACCCCGAC GAGATTGAAA TCGACTTTGA GACCCTGAAG 
CCGTCCACAC TGCGTGAGCT GGAGCGCTAT GTCACCTCCT GTTTGCGGAA GAAAAGGAAA 
CCTCAAGCTG AGAAAGTTGA TGTGATTGCC GGCTCCTCCA AGATGAAGGG CTTCTCGTCC 
TCAGAGTCGG AGAGCTCCAG TGAGTCCAGC TCCTCTGACA GCGAAGACTC CGAAACAGGT 
CCTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRD4 ( XM_011527856.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526158.1 Homo sapiens bromodomain containing 4 (BRD4), transcript variant X3, mRNA.

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CloneID	OHu26888
Clone ID Related Accession (Same CDS sequence)	XM_011527856.1 , XM_011527856.2 , XM_011527856.3 , NM_014299.2
Accession Version	XM_011527856.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2169bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	bromodomain-containing protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527856.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCGG AGAGCGGCCC TGGGACGAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACTT CCCAAATGTC TACAACACAG GCCCAGGCCC AACCCCAGCC AGCCAACGCA 
GCCAGCACCA ACCCCCCGCC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATACCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCA 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATC 
ATTAAAACGC CTATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTACAAC 
AAGCCTGGAG ATGACATAGT CTTAATGGCA GAAGCTCTGG AAAAGCTCTT CTTGCAAAAA 
ATAAATGAGC TACCCACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGTGGGAGGA AAGAAACAGG GACAGCAAAA CCTGGCGTTT CCACGGTACC AAACACAACT 
CAAGCATCGA CTCCTCCGCA GACCCAGACC CCTCAGCCGA ATCCTCCTCC TGTGCAGGCC 
ACGCCTCACC CCTTCCCTGC CGTCACCCCG GACCTCATCG TCCAGACCCC TGTCATGACA 
GTGGTGCCTC CCCAGCCACT GCAGACGCCC CCGCCAGTGC CCCCCCAGCC ACAACCCCCA 
CCCGCTCCAG CTCCCCAGCC CGTACAGAGC CACCCACCCA TCATCGCGGC CACCCCACAG 
CCTGTGAAGA CAAAGAAGGG AGTGAAGAGG AAAGCAGACA CCACCACCCC CACCACCATT 
GACCCCATTC ACGAGCCACC CTCGCTGCCC CCGGAGCCCA AGACCACCAA GCTGGGCCAG 
CGGCGGGAGA GCAGCCGGCC TGTGAAACCT CCAAAGAAGG ACGTGCCCGA CTCTCAGCAG 
CACCCAGCAC CAGAGAAGAG CAGCAAGGTC TCGGAGCAGC TCAAGTGCTG CAGCGGCATC 
CTCAAGGAGA TGTTTGCCAA GAAGCACGCC GCCTACGCCT GGCCCTTCTA CAAGCCTGTG 
GACGTGGAGG CACTGGGCCT ACACGACTAC TGTGACATCA TCAAGCACCC CATGGACATG 
AGCACAATCA AGTCTAAACT GGAGGCCCGT GAGTACCGTG ATGCTCAGGA GTTTGGTGCT 
GACGTCCGAT TGATGTTCTC CAACTGCTAT AAGTACAACC CTCCTGACCA TGAGGTGGTG 
GCCATGGCCC GCAAGCTCCA GGATGTGTTC GAAATGCGCT TTGCCAAGAT GCCGGACGAG 
CCTGAGGAGC CAGTGGTGGC CGTGTCCTCC CCGGCAGTGC CCCCTCCCAC CAAGGTTGTG 
GCCCCGCCCT CATCCAGCGA CAGCAGCAGC GATAGCTCCT CGGACAGTGA CAGTTCGACT 
GATGACTCTG AGGAGGAGCG AGCCCAGCGG CTGGCTGAGC TCCAGGAGCA GCTCAAAGCC 
GTGCACGAGC AGCTTGCAGC CCTCTCTCAG CCCCAGCAGA ACAAACCAAA GAAAAAGGAG 
AAAGACAAGA AGGAAAAGAA AAAAGAAAAG CACAAAAGGA AAGAGGAAGT GGAAGAGAAT 
AAAAAAAGCA AAGCCAAGGA ACCTCCTCCT AAAAAGACGA AGAAAAATAA TAGCAGCAAC 
AGCAATGTGA GCAAGAAGGA GCCAGCGCCC ATGAAGAGCA AGCCCCCTCC CACGTATGAG 
TCGGAGGAAG AGGACAAGTG CAAGCCTATG TCCTATGAGG AGAAGCGGCA GCTCAGCTTG 
GACATCAACA AGCTCCCCGG CGAGAAGCTG GGCCGCGTGG TGCACATCAT CCAGTCACGG 
GAGCCCTCCC TGAAGAATTC CAACCCCGAC GAGATTGAAA TCGACTTTGA GACCCTGAAG 
CCGTCCACAC TGCGTGAGCT GGAGCGCTAT GTCACCTCCT GTTTGCGGAA GAAAAGGAAA 
CCTCAAGCTG AGAAAGTTGA TGTGATTGCC GGCTCCTCCA AGATGAAGGG CTTCTCGTCC 
TCAGAGTCGG AGAGCTCCAG TGAGTCCAGC TCCTCTGACA GCGAAGACTC CGAAACAGGT 
CCTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526158.1
CDS	288..2456
Translation	MSAESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGTAKPGVSTVPNTTQASTPPQTQTPQPNPPPVQATPHPFPAVTPDLIVQTPVMTVVPPQPLQTPPPVPPQPQPPPAPAPQPVQSHPPIIAATPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLPPEPKTTKLGQRRESSRPVKPPKKDVPDSQQHPAPEKSSKVSEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLEAREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVAVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKRKEEVEENKKSKAKEPPPKKTKKNNSSNSNVSKKEPAPMKSKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESSSESSSSDSEDSETGPA

Target ORF information:

RefSeq Version	XM_011527856.2
Organism	Homo sapiens(human)
Definition	Homo sapiens bromodomain containing 4 (BRD4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527856.2

ORF Insert Sequence:

ATGTCTGCGG AGAGCGGCCC TGGGACGAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACTT CCCAAATGTC TACAACACAG GCCCAGGCCC AACCCCAGCC AGCCAACGCA 
GCCAGCACCA ACCCCCCGCC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATACCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCA 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATC 
ATTAAAACGC CTATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTACAAC 
AAGCCTGGAG ATGACATAGT CTTAATGGCA GAAGCTCTGG AAAAGCTCTT CTTGCAAAAA 
ATAAATGAGC TACCCACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGTGGGAGGA AAGAAACAGG GACAGCAAAA CCTGGCGTTT CCACGGTACC AAACACAACT 
CAAGCATCGA CTCCTCCGCA GACCCAGACC CCTCAGCCGA ATCCTCCTCC TGTGCAGGCC 
ACGCCTCACC CCTTCCCTGC CGTCACCCCG GACCTCATCG TCCAGACCCC TGTCATGACA 
GTGGTGCCTC CCCAGCCACT GCAGACGCCC CCGCCAGTGC CCCCCCAGCC ACAACCCCCA 
CCCGCTCCAG CTCCCCAGCC CGTACAGAGC CACCCACCCA TCATCGCGGC CACCCCACAG 
CCTGTGAAGA CAAAGAAGGG AGTGAAGAGG AAAGCAGACA CCACCACCCC CACCACCATT 
GACCCCATTC ACGAGCCACC CTCGCTGCCC CCGGAGCCCA AGACCACCAA GCTGGGCCAG 
CGGCGGGAGA GCAGCCGGCC TGTGAAACCT CCAAAGAAGG ACGTGCCCGA CTCTCAGCAG 
CACCCAGCAC CAGAGAAGAG CAGCAAGGTC TCGGAGCAGC TCAAGTGCTG CAGCGGCATC 
CTCAAGGAGA TGTTTGCCAA GAAGCACGCC GCCTACGCCT GGCCCTTCTA CAAGCCTGTG 
GACGTGGAGG CACTGGGCCT ACACGACTAC TGTGACATCA TCAAGCACCC CATGGACATG 
AGCACAATCA AGTCTAAACT GGAGGCCCGT GAGTACCGTG ATGCTCAGGA GTTTGGTGCT 
GACGTCCGAT TGATGTTCTC CAACTGCTAT AAGTACAACC CTCCTGACCA TGAGGTGGTG 
GCCATGGCCC GCAAGCTCCA GGATGTGTTC GAAATGCGCT TTGCCAAGAT GCCGGACGAG 
CCTGAGGAGC CAGTGGTGGC CGTGTCCTCC CCGGCAGTGC CCCCTCCCAC CAAGGTTGTG 
GCCCCGCCCT CATCCAGCGA CAGCAGCAGC GATAGCTCCT CGGACAGTGA CAGTTCGACT 
GATGACTCTG AGGAGGAGCG AGCCCAGCGG CTGGCTGAGC TCCAGGAGCA GCTCAAAGCC 
GTGCACGAGC AGCTTGCAGC CCTCTCTCAG CCCCAGCAGA ACAAACCAAA GAAAAAGGAG 
AAAGACAAGA AGGAAAAGAA AAAAGAAAAG CACAAAAGGA AAGAGGAAGT GGAAGAGAAT 
AAAAAAAGCA AAGCCAAGGA ACCTCCTCCT AAAAAGACGA AGAAAAATAA TAGCAGCAAC 
AGCAATGTGA GCAAGAAGGA GCCAGCGCCC ATGAAGAGCA AGCCCCCTCC CACGTATGAG 
TCGGAGGAAG AGGACAAGTG CAAGCCTATG TCCTATGAGG AGAAGCGGCA GCTCAGCTTG 
GACATCAACA AGCTCCCCGG CGAGAAGCTG GGCCGCGTGG TGCACATCAT CCAGTCACGG 
GAGCCCTCCC TGAAGAATTC CAACCCCGAC GAGATTGAAA TCGACTTTGA GACCCTGAAG 
CCGTCCACAC TGCGTGAGCT GGAGCGCTAT GTCACCTCCT GTTTGCGGAA GAAAAGGAAA 
CCTCAAGCTG AGAAAGTTGA TGTGATTGCC GGCTCCTCCA AGATGAAGGG CTTCTCGTCC 
TCAGAGTCGG AGAGCTCCAG TGAGTCCAGC TCCTCTGACA GCGAAGACTC CGAAACAGGT 
CCTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRD4 ( XM_011527856.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526158.1 Homo sapiens bromodomain containing 4 (BRD4), transcript variant X2, mRNA.

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CloneID	OHu26888
Clone ID Related Accession (Same CDS sequence)	XM_011527856.1 , XM_011527856.2 , XM_011527856.3 , NM_014299.2
Accession Version	XM_011527856.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2169bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bromodomain-containing protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011527856.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCGG AGAGCGGCCC TGGGACGAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACTT CCCAAATGTC TACAACACAG GCCCAGGCCC AACCCCAGCC AGCCAACGCA 
GCCAGCACCA ACCCCCCGCC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATACCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCA 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATC 
ATTAAAACGC CTATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTACAAC 
AAGCCTGGAG ATGACATAGT CTTAATGGCA GAAGCTCTGG AAAAGCTCTT CTTGCAAAAA 
ATAAATGAGC TACCCACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGTGGGAGGA AAGAAACAGG GACAGCAAAA CCTGGCGTTT CCACGGTACC AAACACAACT 
CAAGCATCGA CTCCTCCGCA GACCCAGACC CCTCAGCCGA ATCCTCCTCC TGTGCAGGCC 
ACGCCTCACC CCTTCCCTGC CGTCACCCCG GACCTCATCG TCCAGACCCC TGTCATGACA 
GTGGTGCCTC CCCAGCCACT GCAGACGCCC CCGCCAGTGC CCCCCCAGCC ACAACCCCCA 
CCCGCTCCAG CTCCCCAGCC CGTACAGAGC CACCCACCCA TCATCGCGGC CACCCCACAG 
CCTGTGAAGA CAAAGAAGGG AGTGAAGAGG AAAGCAGACA CCACCACCCC CACCACCATT 
GACCCCATTC ACGAGCCACC CTCGCTGCCC CCGGAGCCCA AGACCACCAA GCTGGGCCAG 
CGGCGGGAGA GCAGCCGGCC TGTGAAACCT CCAAAGAAGG ACGTGCCCGA CTCTCAGCAG 
CACCCAGCAC CAGAGAAGAG CAGCAAGGTC TCGGAGCAGC TCAAGTGCTG CAGCGGCATC 
CTCAAGGAGA TGTTTGCCAA GAAGCACGCC GCCTACGCCT GGCCCTTCTA CAAGCCTGTG 
GACGTGGAGG CACTGGGCCT ACACGACTAC TGTGACATCA TCAAGCACCC CATGGACATG 
AGCACAATCA AGTCTAAACT GGAGGCCCGT GAGTACCGTG ATGCTCAGGA GTTTGGTGCT 
GACGTCCGAT TGATGTTCTC CAACTGCTAT AAGTACAACC CTCCTGACCA TGAGGTGGTG 
GCCATGGCCC GCAAGCTCCA GGATGTGTTC GAAATGCGCT TTGCCAAGAT GCCGGACGAG 
CCTGAGGAGC CAGTGGTGGC CGTGTCCTCC CCGGCAGTGC CCCCTCCCAC CAAGGTTGTG 
GCCCCGCCCT CATCCAGCGA CAGCAGCAGC GATAGCTCCT CGGACAGTGA CAGTTCGACT 
GATGACTCTG AGGAGGAGCG AGCCCAGCGG CTGGCTGAGC TCCAGGAGCA GCTCAAAGCC 
GTGCACGAGC AGCTTGCAGC CCTCTCTCAG CCCCAGCAGA ACAAACCAAA GAAAAAGGAG 
AAAGACAAGA AGGAAAAGAA AAAAGAAAAG CACAAAAGGA AAGAGGAAGT GGAAGAGAAT 
AAAAAAAGCA AAGCCAAGGA ACCTCCTCCT AAAAAGACGA AGAAAAATAA TAGCAGCAAC 
AGCAATGTGA GCAAGAAGGA GCCAGCGCCC ATGAAGAGCA AGCCCCCTCC CACGTATGAG 
TCGGAGGAAG AGGACAAGTG CAAGCCTATG TCCTATGAGG AGAAGCGGCA GCTCAGCTTG 
GACATCAACA AGCTCCCCGG CGAGAAGCTG GGCCGCGTGG TGCACATCAT CCAGTCACGG 
GAGCCCTCCC TGAAGAATTC CAACCCCGAC GAGATTGAAA TCGACTTTGA GACCCTGAAG 
CCGTCCACAC TGCGTGAGCT GGAGCGCTAT GTCACCTCCT GTTTGCGGAA GAAAAGGAAA 
CCTCAAGCTG AGAAAGTTGA TGTGATTGCC GGCTCCTCCA AGATGAAGGG CTTCTCGTCC 
TCAGAGTCGG AGAGCTCCAG TGAGTCCAGC TCCTCTGACA GCGAAGACTC CGAAACAGGT 
CCTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526158.1
CDS	72..2240
Translation	MSAESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGTAKPGVSTVPNTTQASTPPQTQTPQPNPPPVQATPHPFPAVTPDLIVQTPVMTVVPPQPLQTPPPVPPQPQPPPAPAPQPVQSHPPIIAATPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLPPEPKTTKLGQRRESSRPVKPPKKDVPDSQQHPAPEKSSKVSEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLEAREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVAVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKRKEEVEENKKSKAKEPPPKKTKKNNSSNSNVSKKEPAPMKSKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESSSESSSSDSEDSETGPA

Target ORF information:

RefSeq Version	XM_011527856.3
Organism	Homo sapiens(human)
Definition	Homo sapiens bromodomain containing 4 (BRD4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527856.3

ORF Insert Sequence:

ATGTCTGCGG AGAGCGGCCC TGGGACGAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACTT CCCAAATGTC TACAACACAG GCCCAGGCCC AACCCCAGCC AGCCAACGCA 
GCCAGCACCA ACCCCCCGCC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATACCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCA 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATC 
ATTAAAACGC CTATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTACAAC 
AAGCCTGGAG ATGACATAGT CTTAATGGCA GAAGCTCTGG AAAAGCTCTT CTTGCAAAAA 
ATAAATGAGC TACCCACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGTGGGAGGA AAGAAACAGG GACAGCAAAA CCTGGCGTTT CCACGGTACC AAACACAACT 
CAAGCATCGA CTCCTCCGCA GACCCAGACC CCTCAGCCGA ATCCTCCTCC TGTGCAGGCC 
ACGCCTCACC CCTTCCCTGC CGTCACCCCG GACCTCATCG TCCAGACCCC TGTCATGACA 
GTGGTGCCTC CCCAGCCACT GCAGACGCCC CCGCCAGTGC CCCCCCAGCC ACAACCCCCA 
CCCGCTCCAG CTCCCCAGCC CGTACAGAGC CACCCACCCA TCATCGCGGC CACCCCACAG 
CCTGTGAAGA CAAAGAAGGG AGTGAAGAGG AAAGCAGACA CCACCACCCC CACCACCATT 
GACCCCATTC ACGAGCCACC CTCGCTGCCC CCGGAGCCCA AGACCACCAA GCTGGGCCAG 
CGGCGGGAGA GCAGCCGGCC TGTGAAACCT CCAAAGAAGG ACGTGCCCGA CTCTCAGCAG 
CACCCAGCAC CAGAGAAGAG CAGCAAGGTC TCGGAGCAGC TCAAGTGCTG CAGCGGCATC 
CTCAAGGAGA TGTTTGCCAA GAAGCACGCC GCCTACGCCT GGCCCTTCTA CAAGCCTGTG 
GACGTGGAGG CACTGGGCCT ACACGACTAC TGTGACATCA TCAAGCACCC CATGGACATG 
AGCACAATCA AGTCTAAACT GGAGGCCCGT GAGTACCGTG ATGCTCAGGA GTTTGGTGCT 
GACGTCCGAT TGATGTTCTC CAACTGCTAT AAGTACAACC CTCCTGACCA TGAGGTGGTG 
GCCATGGCCC GCAAGCTCCA GGATGTGTTC GAAATGCGCT TTGCCAAGAT GCCGGACGAG 
CCTGAGGAGC CAGTGGTGGC CGTGTCCTCC CCGGCAGTGC CCCCTCCCAC CAAGGTTGTG 
GCCCCGCCCT CATCCAGCGA CAGCAGCAGC GATAGCTCCT CGGACAGTGA CAGTTCGACT 
GATGACTCTG AGGAGGAGCG AGCCCAGCGG CTGGCTGAGC TCCAGGAGCA GCTCAAAGCC 
GTGCACGAGC AGCTTGCAGC CCTCTCTCAG CCCCAGCAGA ACAAACCAAA GAAAAAGGAG 
AAAGACAAGA AGGAAAAGAA AAAAGAAAAG CACAAAAGGA AAGAGGAAGT GGAAGAGAAT 
AAAAAAAGCA AAGCCAAGGA ACCTCCTCCT AAAAAGACGA AGAAAAATAA TAGCAGCAAC 
AGCAATGTGA GCAAGAAGGA GCCAGCGCCC ATGAAGAGCA AGCCCCCTCC CACGTATGAG 
TCGGAGGAAG AGGACAAGTG CAAGCCTATG TCCTATGAGG AGAAGCGGCA GCTCAGCTTG 
GACATCAACA AGCTCCCCGG CGAGAAGCTG GGCCGCGTGG TGCACATCAT CCAGTCACGG 
GAGCCCTCCC TGAAGAATTC CAACCCCGAC GAGATTGAAA TCGACTTTGA GACCCTGAAG 
CCGTCCACAC TGCGTGAGCT GGAGCGCTAT GTCACCTCCT GTTTGCGGAA GAAAAGGAAA 
CCTCAAGCTG AGAAAGTTGA TGTGATTGCC GGCTCCTCCA AGATGAAGGG CTTCTCGTCC 
TCAGAGTCGG AGAGCTCCAG TGAGTCCAGC TCCTCTGACA GCGAAGACTC CGAAACAGGT 
CCTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRD4 ( NM_001330384.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317313.1 Homo sapiens bromodomain containing 4 (BRD4), transcript variant 3, mRNA.

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CloneID	OHu66214
Clone ID Related Accession (Same CDS sequence)	NM_001330384.1 , NM_001330384.2
Accession Version	NM_001330384.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	bromodomain-containing protein 4 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005776.1 and BC035266.1. On Aug 24, 2016 this sequence version replaced XM_011527855.1. Transcript Variant: This variant (short) differs in the 3' UTR and coding region compared to variant long. The resulting isoform (short) is shorter and has a distinct C-terminus compared to isoform long. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC035266.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCGG AGAGCGGCCC TGGGACGAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACTT CCCAAATGTC TACAACACAG GCCCAGGCCC AACCCCAGCC AGCCAACGCA 
GCCAGCACCA ACCCCCCGCC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATACCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCA 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATC 
ATTAAAACGC CTATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTACAAC 
AAGCCTGGAG ATGACATAGT CTTAATGGCA GAAGCTCTGG AAAAGCTCTT CTTGCAAAAA 
ATAAATGAGC TACCCACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGTGGGAGGA AAGAAACAGG GACAGCAAAA CCTGGCGTTT CCACGGTACC AAACACAACT 
CAAGCATCGA CTCCTCCGCA GACCCAGACC CCTCAGCCGA ATCCTCCTCC TGTGCAGGCC 
ACGCCTCACC CCTTCCCTGC CGTCACCCCG GACCTCATCG TCCAGACCCC TGTCATGACA 
GTGGTGCCTC CCCAGCCACT GCAGACGCCC CCGCCAGTGC CCCCCCAGCC ACAACCCCCA 
CCCGCTCCAG CTCCCCAGCC CGTACAGAGC CACCCACCCA TCATCGCGGC CACCCCACAG 
CCTGTGAAGA CAAAGAAGGG AGTGAAGAGG AAAGCAGACA CCACCACCCC CACCACCATT 
GACCCCATTC ACGAGCCACC CTCGCTGCCC CCGGAGCCCA AGACCACCAA GCTGGGCCAG 
CGGCGGGAGA GCAGCCGGCC TGTGAAACCT CCAAAGAAGG ACGTGCCCGA CTCTCAGCAG 
CACCCAGCAC CAGAGAAGAG CAGCAAGGTC TCGGAGCAGC TCAAGTGCTG CAGCGGCATC 
CTCAAGGAGA TGTTTGCCAA GAAGCACGCC GCCTACGCCT GGCCCTTCTA CAAGCCTGTG 
GACGTGGAGG CACTGGGCCT ACACGACTAC TGTGACATCA TCAAGCACCC CATGGACATG 
AGCACAATCA AGTCTAAACT GGAGGCCCGT GAGTACCGTG ATGCTCAGGA GTTTGGTGCT 
GACGTCCGAT TGATGTTCTC CAACTGCTAT AAGTACAACC CTCCTGACCA TGAGGTGGTG 
GCCATGGCCC GCAAGCTCCA GGATGTGTTC GAAATGCGCT TTGCCAAGAT GCCGGACGAG 
CCTGAGGAGC CAGTGGTGGC CGTGTCCTCC CCGGCAGTGC CCCCTCCCAC CAAGGTTGTG 
GCCCCGCCCT CATCCAGCGA CAGCAGCAGC GATAGCTCCT CGGACAGTGA CAGTTCGACT 
GATGACTCTG AGGAGGAGCG AGCCCAGCGG CTGGCTGAGC TCCAGGAGCA GCTCAAAGCC 
GTGCACGAGC AGCTTGCAGC CCTCTCTCAG CCCCAGCAGA ACAAACCAAA GAAAAAGGAG 
AAAGACAAGA AGGAAAAGAA AAAAGAAAAG CACAAAAGGA AAGAGGAAGT GGAAGAGAAT 
AAAAAAAGCA AAGCCAAGGA ACCTCCTCCT AAAAAGACGA AGAAAAATAA TAGCAGCAAC 
AGCAATGTGA GCAAGAAGGA GCCAGCGCCC ATGAAGAGCA AGCCCCCTCC CACGTATGAG 
TCGGAGGAAG AGGACAAGTG CAAGCCTATG TCCTATGAGG AGAAGCGGCA GCTCAGCTTG 
GACATCAACA AGCTCCCCGG CGAGAAGCTG GGCCGCGTGG TGCACATCAT CCAGTCACGG 
GAGCCCTCCC TGAAGAATTC CAACCCCGAC GAGATTGAAA TCGACTTTGA GACCCTGAAG 
CCGTCCACAC TGCGTGAGCT GGAGCGCTAT GTCACCTCCT GTTTGCGGAA GAAAAGGAAA 
CCTCAAGCTG AGAAAGTTGA TGTGATTGCC GGCTCCTCCA AGATGAAGGG CTTCTCGTCC 
TCAGAGTCGG AGAGCTCCAG TGAGTCCAGC TCCTCTGACA GCGAAGACTC CGAAACAGCT 
TTCTGCACCA GTGGAGACTT CGTGTCCCCA GGGCCTTCCC CGTATCACAG TCACGTGCAG 
TGCGGCCGCT TCAGGGAGAT GCTTCGCTGG TTTCTGGTGG ATGTGGAGCA GACTGCAGCT 
GGCCAGCCGC ATCGCCAGTC TGCTGCTGGC CCTGCCATCA CCTGGGCCCC AGCCATTGCC 
TACCCCTCCC CAGAGTGTGC TCGTTGCTGT GTTGGCTGCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317313.1
CDS	289..2673
Translation	MSAESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGTAKPGVSTVPNTTQASTPPQTQTPQPNPPPVQATPHPFPAVTPDLIVQTPVMTVVPPQPLQTPPPVPPQPQPPPAPAPQPVQSHPPIIAATPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLPPEPKTTKLGQRRESSRPVKPPKKDVPDSQQHPAPEKSSKVSEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLEAREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVAVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKRKEEVEENKKSKAKEPPPKKTKKNNSSNSNVSKKEPAPMKSKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESSSESSSSDSEDSETAFCTSGDFVSPGPSPYHSHVQCGRFREMLRWFLVDVEQTAAGQPHRQSAAGPAITWAPAIAYPSPECARCCVGCS

Target ORF information:

RefSeq Version	NM_001330384.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bromodomain containing 4 (BRD4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330384.1

ORF Insert Sequence:

ATGTCTGCGG AGAGCGGCCC TGGGACGAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACTT CCCAAATGTC TACAACACAG GCCCAGGCCC AACCCCAGCC AGCCAACGCA 
GCCAGCACCA ACCCCCCGCC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATACCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCA 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATC 
ATTAAAACGC CTATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTACAAC 
AAGCCTGGAG ATGACATAGT CTTAATGGCA GAAGCTCTGG AAAAGCTCTT CTTGCAAAAA 
ATAAATGAGC TACCCACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGTGGGAGGA AAGAAACAGG GACAGCAAAA CCTGGCGTTT CCACGGTACC AAACACAACT 
CAAGCATCGA CTCCTCCGCA GACCCAGACC CCTCAGCCGA ATCCTCCTCC TGTGCAGGCC 
ACGCCTCACC CCTTCCCTGC CGTCACCCCG GACCTCATCG TCCAGACCCC TGTCATGACA 
GTGGTGCCTC CCCAGCCACT GCAGACGCCC CCGCCAGTGC CCCCCCAGCC ACAACCCCCA 
CCCGCTCCAG CTCCCCAGCC CGTACAGAGC CACCCACCCA TCATCGCGGC CACCCCACAG 
CCTGTGAAGA CAAAGAAGGG AGTGAAGAGG AAAGCAGACA CCACCACCCC CACCACCATT 
GACCCCATTC ACGAGCCACC CTCGCTGCCC CCGGAGCCCA AGACCACCAA GCTGGGCCAG 
CGGCGGGAGA GCAGCCGGCC TGTGAAACCT CCAAAGAAGG ACGTGCCCGA CTCTCAGCAG 
CACCCAGCAC CAGAGAAGAG CAGCAAGGTC TCGGAGCAGC TCAAGTGCTG CAGCGGCATC 
CTCAAGGAGA TGTTTGCCAA GAAGCACGCC GCCTACGCCT GGCCCTTCTA CAAGCCTGTG 
GACGTGGAGG CACTGGGCCT ACACGACTAC TGTGACATCA TCAAGCACCC CATGGACATG 
AGCACAATCA AGTCTAAACT GGAGGCCCGT GAGTACCGTG ATGCTCAGGA GTTTGGTGCT 
GACGTCCGAT TGATGTTCTC CAACTGCTAT AAGTACAACC CTCCTGACCA TGAGGTGGTG 
GCCATGGCCC GCAAGCTCCA GGATGTGTTC GAAATGCGCT TTGCCAAGAT GCCGGACGAG 
CCTGAGGAGC CAGTGGTGGC CGTGTCCTCC CCGGCAGTGC CCCCTCCCAC CAAGGTTGTG 
GCCCCGCCCT CATCCAGCGA CAGCAGCAGC GATAGCTCCT CGGACAGTGA CAGTTCGACT 
GATGACTCTG AGGAGGAGCG AGCCCAGCGG CTGGCTGAGC TCCAGGAGCA GCTCAAAGCC 
GTGCACGAGC AGCTTGCAGC CCTCTCTCAG CCCCAGCAGA ACAAACCAAA GAAAAAGGAG 
AAAGACAAGA AGGAAAAGAA AAAAGAAAAG CACAAAAGGA AAGAGGAAGT GGAAGAGAAT 
AAAAAAAGCA AAGCCAAGGA ACCTCCTCCT AAAAAGACGA AGAAAAATAA TAGCAGCAAC 
AGCAATGTGA GCAAGAAGGA GCCAGCGCCC ATGAAGAGCA AGCCCCCTCC CACGTATGAG 
TCGGAGGAAG AGGACAAGTG CAAGCCTATG TCCTATGAGG AGAAGCGGCA GCTCAGCTTG 
GACATCAACA AGCTCCCCGG CGAGAAGCTG GGCCGCGTGG TGCACATCAT CCAGTCACGG 
GAGCCCTCCC TGAAGAATTC CAACCCCGAC GAGATTGAAA TCGACTTTGA GACCCTGAAG 
CCGTCCACAC TGCGTGAGCT GGAGCGCTAT GTCACCTCCT GTTTGCGGAA GAAAAGGAAA 
CCTCAAGCTG AGAAAGTTGA TGTGATTGCC GGCTCCTCCA AGATGAAGGG CTTCTCGTCC 
TCAGAGTCGG AGAGCTCCAG TGAGTCCAGC TCCTCTGACA GCGAAGACTC CGAAACAGCT 
TTCTGCACCA GTGGAGACTT CGTGTCCCCA GGGCCTTCCC CGTATCACAG TCACGTGCAG 
TGCGGCCGCT TCAGGGAGAT GCTTCGCTGG TTTCTGGTGG ATGTGGAGCA GACTGCAGCT 
GGCCAGCCGC ATCGCCAGTC TGCTGCTGGC CCTGCCATCA CCTGGGCCCC AGCCATTGCC 
TACCCCTCCC CAGAGTGTGC TCGTTGCTGT GTTGGCTGCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRD4 ( NM_001330384.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317313.1 Homo sapiens bromodomain containing 4 (BRD4), transcript variant 3, mRNA.

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CloneID	OHu66214
Clone ID Related Accession (Same CDS sequence)	NM_001330384.1 , NM_001330384.2
Accession Version	NM_001330384.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	bromodomain-containing protein 4 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005776.1, BC035266.1 and AC004798.1. On Jun 2, 2019 this sequence version replaced NM_001330384.1. Transcript Variant: This variant (short) differs in the 3' UTR and coding region compared to variant long. The resulting isoform (short) is shorter and has a distinct C-terminus compared to isoform long. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC035266.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCGG AGAGCGGCCC TGGGACGAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACTT CCCAAATGTC TACAACACAG GCCCAGGCCC AACCCCAGCC AGCCAACGCA 
GCCAGCACCA ACCCCCCGCC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATACCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCA 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATC 
ATTAAAACGC CTATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTACAAC 
AAGCCTGGAG ATGACATAGT CTTAATGGCA GAAGCTCTGG AAAAGCTCTT CTTGCAAAAA 
ATAAATGAGC TACCCACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGTGGGAGGA AAGAAACAGG GACAGCAAAA CCTGGCGTTT CCACGGTACC AAACACAACT 
CAAGCATCGA CTCCTCCGCA GACCCAGACC CCTCAGCCGA ATCCTCCTCC TGTGCAGGCC 
ACGCCTCACC CCTTCCCTGC CGTCACCCCG GACCTCATCG TCCAGACCCC TGTCATGACA 
GTGGTGCCTC CCCAGCCACT GCAGACGCCC CCGCCAGTGC CCCCCCAGCC ACAACCCCCA 
CCCGCTCCAG CTCCCCAGCC CGTACAGAGC CACCCACCCA TCATCGCGGC CACCCCACAG 
CCTGTGAAGA CAAAGAAGGG AGTGAAGAGG AAAGCAGACA CCACCACCCC CACCACCATT 
GACCCCATTC ACGAGCCACC CTCGCTGCCC CCGGAGCCCA AGACCACCAA GCTGGGCCAG 
CGGCGGGAGA GCAGCCGGCC TGTGAAACCT CCAAAGAAGG ACGTGCCCGA CTCTCAGCAG 
CACCCAGCAC CAGAGAAGAG CAGCAAGGTC TCGGAGCAGC TCAAGTGCTG CAGCGGCATC 
CTCAAGGAGA TGTTTGCCAA GAAGCACGCC GCCTACGCCT GGCCCTTCTA CAAGCCTGTG 
GACGTGGAGG CACTGGGCCT ACACGACTAC TGTGACATCA TCAAGCACCC CATGGACATG 
AGCACAATCA AGTCTAAACT GGAGGCCCGT GAGTACCGTG ATGCTCAGGA GTTTGGTGCT 
GACGTCCGAT TGATGTTCTC CAACTGCTAT AAGTACAACC CTCCTGACCA TGAGGTGGTG 
GCCATGGCCC GCAAGCTCCA GGATGTGTTC GAAATGCGCT TTGCCAAGAT GCCGGACGAG 
CCTGAGGAGC CAGTGGTGGC CGTGTCCTCC CCGGCAGTGC CCCCTCCCAC CAAGGTTGTG 
GCCCCGCCCT CATCCAGCGA CAGCAGCAGC GATAGCTCCT CGGACAGTGA CAGTTCGACT 
GATGACTCTG AGGAGGAGCG AGCCCAGCGG CTGGCTGAGC TCCAGGAGCA GCTCAAAGCC 
GTGCACGAGC AGCTTGCAGC CCTCTCTCAG CCCCAGCAGA ACAAACCAAA GAAAAAGGAG 
AAAGACAAGA AGGAAAAGAA AAAAGAAAAG CACAAAAGGA AAGAGGAAGT GGAAGAGAAT 
AAAAAAAGCA AAGCCAAGGA ACCTCCTCCT AAAAAGACGA AGAAAAATAA TAGCAGCAAC 
AGCAATGTGA GCAAGAAGGA GCCAGCGCCC ATGAAGAGCA AGCCCCCTCC CACGTATGAG 
TCGGAGGAAG AGGACAAGTG CAAGCCTATG TCCTATGAGG AGAAGCGGCA GCTCAGCTTG 
GACATCAACA AGCTCCCCGG CGAGAAGCTG GGCCGCGTGG TGCACATCAT CCAGTCACGG 
GAGCCCTCCC TGAAGAATTC CAACCCCGAC GAGATTGAAA TCGACTTTGA GACCCTGAAG 
CCGTCCACAC TGCGTGAGCT GGAGCGCTAT GTCACCTCCT GTTTGCGGAA GAAAAGGAAA 
CCTCAAGCTG AGAAAGTTGA TGTGATTGCC GGCTCCTCCA AGATGAAGGG CTTCTCGTCC 
TCAGAGTCGG AGAGCTCCAG TGAGTCCAGC TCCTCTGACA GCGAAGACTC CGAAACAGCT 
TTCTGCACCA GTGGAGACTT CGTGTCCCCA GGGCCTTCCC CGTATCACAG TCACGTGCAG 
TGCGGCCGCT TCAGGGAGAT GCTTCGCTGG TTTCTGGTGG ATGTGGAGCA GACTGCAGCT 
GGCCAGCCGC ATCGCCAGTC TGCTGCTGGC CCTGCCATCA CCTGGGCCCC AGCCATTGCC 
TACCCCTCCC CAGAGTGTGC TCGTTGCTGT GTTGGCTGCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317313.1
CDS	285..2669
Translation	MSAESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGTAKPGVSTVPNTTQASTPPQTQTPQPNPPPVQATPHPFPAVTPDLIVQTPVMTVVPPQPLQTPPPVPPQPQPPPAPAPQPVQSHPPIIAATPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLPPEPKTTKLGQRRESSRPVKPPKKDVPDSQQHPAPEKSSKVSEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLEAREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVAVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKRKEEVEENKKSKAKEPPPKKTKKNNSSNSNVSKKEPAPMKSKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESSSESSSSDSEDSETAFCTSGDFVSPGPSPYHSHVQCGRFREMLRWFLVDVEQTAAGQPHRQSAAGPAITWAPAIAYPSPECARCCVGCS

Target ORF information:

RefSeq Version	NM_001330384.2
Organism	Homo sapiens(human)
Definition	Homo sapiens bromodomain containing 4 (BRD4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330384.2

ORF Insert Sequence:

ATGTCTGCGG AGAGCGGCCC TGGGACGAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACTT CCCAAATGTC TACAACACAG GCCCAGGCCC AACCCCAGCC AGCCAACGCA 
GCCAGCACCA ACCCCCCGCC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATACCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCA 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATC 
ATTAAAACGC CTATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTACAAC 
AAGCCTGGAG ATGACATAGT CTTAATGGCA GAAGCTCTGG AAAAGCTCTT CTTGCAAAAA 
ATAAATGAGC TACCCACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGTGGGAGGA AAGAAACAGG GACAGCAAAA CCTGGCGTTT CCACGGTACC AAACACAACT 
CAAGCATCGA CTCCTCCGCA GACCCAGACC CCTCAGCCGA ATCCTCCTCC TGTGCAGGCC 
ACGCCTCACC CCTTCCCTGC CGTCACCCCG GACCTCATCG TCCAGACCCC TGTCATGACA 
GTGGTGCCTC CCCAGCCACT GCAGACGCCC CCGCCAGTGC CCCCCCAGCC ACAACCCCCA 
CCCGCTCCAG CTCCCCAGCC CGTACAGAGC CACCCACCCA TCATCGCGGC CACCCCACAG 
CCTGTGAAGA CAAAGAAGGG AGTGAAGAGG AAAGCAGACA CCACCACCCC CACCACCATT 
GACCCCATTC ACGAGCCACC CTCGCTGCCC CCGGAGCCCA AGACCACCAA GCTGGGCCAG 
CGGCGGGAGA GCAGCCGGCC TGTGAAACCT CCAAAGAAGG ACGTGCCCGA CTCTCAGCAG 
CACCCAGCAC CAGAGAAGAG CAGCAAGGTC TCGGAGCAGC TCAAGTGCTG CAGCGGCATC 
CTCAAGGAGA TGTTTGCCAA GAAGCACGCC GCCTACGCCT GGCCCTTCTA CAAGCCTGTG 
GACGTGGAGG CACTGGGCCT ACACGACTAC TGTGACATCA TCAAGCACCC CATGGACATG 
AGCACAATCA AGTCTAAACT GGAGGCCCGT GAGTACCGTG ATGCTCAGGA GTTTGGTGCT 
GACGTCCGAT TGATGTTCTC CAACTGCTAT AAGTACAACC CTCCTGACCA TGAGGTGGTG 
GCCATGGCCC GCAAGCTCCA GGATGTGTTC GAAATGCGCT TTGCCAAGAT GCCGGACGAG 
CCTGAGGAGC CAGTGGTGGC CGTGTCCTCC CCGGCAGTGC CCCCTCCCAC CAAGGTTGTG 
GCCCCGCCCT CATCCAGCGA CAGCAGCAGC GATAGCTCCT CGGACAGTGA CAGTTCGACT 
GATGACTCTG AGGAGGAGCG AGCCCAGCGG CTGGCTGAGC TCCAGGAGCA GCTCAAAGCC 
GTGCACGAGC AGCTTGCAGC CCTCTCTCAG CCCCAGCAGA ACAAACCAAA GAAAAAGGAG 
AAAGACAAGA AGGAAAAGAA AAAAGAAAAG CACAAAAGGA AAGAGGAAGT GGAAGAGAAT 
AAAAAAAGCA AAGCCAAGGA ACCTCCTCCT AAAAAGACGA AGAAAAATAA TAGCAGCAAC 
AGCAATGTGA GCAAGAAGGA GCCAGCGCCC ATGAAGAGCA AGCCCCCTCC CACGTATGAG 
TCGGAGGAAG AGGACAAGTG CAAGCCTATG TCCTATGAGG AGAAGCGGCA GCTCAGCTTG 
GACATCAACA AGCTCCCCGG CGAGAAGCTG GGCCGCGTGG TGCACATCAT CCAGTCACGG 
GAGCCCTCCC TGAAGAATTC CAACCCCGAC GAGATTGAAA TCGACTTTGA GACCCTGAAG 
CCGTCCACAC TGCGTGAGCT GGAGCGCTAT GTCACCTCCT GTTTGCGGAA GAAAAGGAAA 
CCTCAAGCTG AGAAAGTTGA TGTGATTGCC GGCTCCTCCA AGATGAAGGG CTTCTCGTCC 
TCAGAGTCGG AGAGCTCCAG TGAGTCCAGC TCCTCTGACA GCGAAGACTC CGAAACAGCT 
TTCTGCACCA GTGGAGACTT CGTGTCCCCA GGGCCTTCCC CGTATCACAG TCACGTGCAG 
TGCGGCCGCT TCAGGGAGAT GCTTCGCTGG TTTCTGGTGG ATGTGGAGCA GACTGCAGCT 
GGCCAGCCGC ATCGCCAGTC TGCTGCTGGC CCTGCCATCA CCTGGGCCCC AGCCATTGCC 
TACCCCTCCC CAGAGTGTGC TCGTTGCTGT GTTGGCTGCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRD4 ( NM_058243.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_490597.1 Homo sapiens bromodomain containing 4 (BRD4), transcript variant long, mRNA.

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CloneID	OHu27190
Clone ID Related Accession (Same CDS sequence)	XM_011527854.1 , XM_011527854.2 , NM_058243.2
Accession Version	NM_058243.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4089bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Homo sapiens(human)
Product	bromodomain-containing protein 4 isoform long
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF386649.1 and BC035266.1. On Aug 18, 2006 this sequence version replaced NM_058243.1. Transcript Variant: This variant (long) encodes the full length isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF386649.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END##

ATGTCTGCGG AGAGCGGCCC TGGGACGAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACTT CCCAAATGTC TACAACACAG GCCCAGGCCC AACCCCAGCC AGCCAACGCA 
GCCAGCACCA ACCCCCCGCC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATACCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCA 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATC 
ATTAAAACGC CTATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTACAAC 
AAGCCTGGAG ATGACATAGT CTTAATGGCA GAAGCTCTGG AAAAGCTCTT CTTGCAAAAA 
ATAAATGAGC TACCCACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGTGGGAGGA AAGAAACAGG GACAGCAAAA CCTGGCGTTT CCACGGTACC AAACACAACT 
CAAGCATCGA CTCCTCCGCA GACCCAGACC CCTCAGCCGA ATCCTCCTCC TGTGCAGGCC 
ACGCCTCACC CCTTCCCTGC CGTCACCCCG GACCTCATCG TCCAGACCCC TGTCATGACA 
GTGGTGCCTC CCCAGCCACT GCAGACGCCC CCGCCAGTGC CCCCCCAGCC ACAACCCCCA 
CCCGCTCCAG CTCCCCAGCC CGTACAGAGC CACCCACCCA TCATCGCGGC CACCCCACAG 
CCTGTGAAGA CAAAGAAGGG AGTGAAGAGG AAAGCAGACA CCACCACCCC CACCACCATT 
GACCCCATTC ACGAGCCACC CTCGCTGCCC CCGGAGCCCA AGACCACCAA GCTGGGCCAG 
CGGCGGGAGA GCAGCCGGCC TGTGAAACCT CCAAAGAAGG ACGTGCCCGA CTCTCAGCAG 
CACCCAGCAC CAGAGAAGAG CAGCAAGGTC TCGGAGCAGC TCAAGTGCTG CAGCGGCATC 
CTCAAGGAGA TGTTTGCCAA GAAGCACGCC GCCTACGCCT GGCCCTTCTA CAAGCCTGTG 
GACGTGGAGG CACTGGGCCT ACACGACTAC TGTGACATCA TCAAGCACCC CATGGACATG 
AGCACAATCA AGTCTAAACT GGAGGCCCGT GAGTACCGTG ATGCTCAGGA GTTTGGTGCT 
GACGTCCGAT TGATGTTCTC CAACTGCTAT AAGTACAACC CTCCTGACCA TGAGGTGGTG 
GCCATGGCCC GCAAGCTCCA GGATGTGTTC GAAATGCGCT TTGCCAAGAT GCCGGACGAG 
CCTGAGGAGC CAGTGGTGGC CGTGTCCTCC CCGGCAGTGC CCCCTCCCAC CAAGGTTGTG 
GCCCCGCCCT CATCCAGCGA CAGCAGCAGC GATAGCTCCT CGGACAGTGA CAGTTCGACT 
GATGACTCTG AGGAGGAGCG AGCCCAGCGG CTGGCTGAGC TCCAGGAGCA GCTCAAAGCC 
GTGCACGAGC AGCTTGCAGC CCTCTCTCAG CCCCAGCAGA ACAAACCAAA GAAAAAGGAG 
AAAGACAAGA AGGAAAAGAA AAAAGAAAAG CACAAAAGGA AAGAGGAAGT GGAAGAGAAT 
AAAAAAAGCA AAGCCAAGGA ACCTCCTCCT AAAAAGACGA AGAAAAATAA TAGCAGCAAC 
AGCAATGTGA GCAAGAAGGA GCCAGCGCCC ATGAAGAGCA AGCCCCCTCC CACGTATGAG 
TCGGAGGAAG AGGACAAGTG CAAGCCTATG TCCTATGAGG AGAAGCGGCA GCTCAGCTTG 
GACATCAACA AGCTCCCCGG CGAGAAGCTG GGCCGCGTGG TGCACATCAT CCAGTCACGG 
GAGCCCTCCC TGAAGAATTC CAACCCCGAC GAGATTGAAA TCGACTTTGA GACCCTGAAG 
CCGTCCACAC TGCGTGAGCT GGAGCGCTAT GTCACCTCCT GTTTGCGGAA GAAAAGGAAA 
CCTCAAGCTG AGAAAGTTGA TGTGATTGCC GGCTCCTCCA AGATGAAGGG CTTCTCGTCC 
TCAGAGTCGG AGAGCTCCAG TGAGTCCAGC TCCTCTGACA GCGAAGACTC CGAAACAGAG 
ATGGCTCCGA AGTCAAAAAA GAAGGGGCAC CCCGGGAGGG AGCAGAAGAA GCACCATCAT 
CACCACCATC AGCAGATGCA GCAGGCCCCG GCTCCTGTGC CCCAGCAGCC GCCCCCGCCT 
CCCCAGCAGC CCCCACCGCC TCCACCTCCG CAGCAGCAAC AGCAGCCGCC ACCCCCGCCT 
CCCCCACCCT CCATGCCGCA GCAGGCAGCC CCGGCGATGA AGTCCTCGCC CCCACCCTTC 
ATTGCCACCC AGGTGCCCGT CCTGGAGCCC CAGCTCCCAG GCAGCGTCTT TGACCCCATC 
GGCCACTTCA CCCAGCCCAT CCTGCACCTG CCGCAGCCTG AGCTGCCCCC TCACCTGCCC 
CAGCCGCCTG AGCACAGCAC TCCACCCCAT CTCAACCAGC ACGCAGTGGT CTCTCCTCCA 
GCTTTGCACA ACGCACTACC CCAGCAGCCA TCACGGCCCA GCAACCGAGC CGCTGCCCTG 
CCTCCCAAGC CCGCCCGGCC CCCAGCCGTG TCACCAGCCT TGACCCAAAC ACCCCTGCTC 
CCACAGCCCC CCATGGCCCA ACCCCCCCAA GTGCTGCTGG AGGATGAAGA GCCACCTGCC 
CCACCCCTCA CCTCCATGCA GATGCAGCTG TACCTGCAGC AGCTGCAGAA GGTGCAGCCC 
CCTACGCCGC TACTCCCTTC CGTGAAGGTG CAGTCCCAGC CCCCACCCCC CCTGCCGCCC 
CCACCCCACC CCTCTGTGCA GCAGCAGCTG CAGCAGCAGC CGCCACCACC CCCACCACCC 
CAGCCCCAGC CTCCACCCCA GCAGCAGCAT CAGCCCCCTC CACGGCCCGT GCACTTGCAG 
CCCATGCAGT TTTCCACCCA CATCCAACAG CCCCCGCCAC CCCAGGGCCA GCAGCCCCCC 
CATCCGCCCC CAGGCCAGCA GCCACCCCCG CCGCAGCCTG CCAAGCCTCA GCAAGTCATC 
CAGCACCACC ATTCACCCCG GCACCACAAG TCGGACCCCT ACTCAACCGG TCACCTCCGC 
GAAGCCCCCT CCCCGCTTAT GATACATTCC CCCCAGATGT CACAGTTCCA GAGCCTGACC 
CACCAGTCTC CACCCCAGCA AAACGTCCAG CCTAAGAAAC AGGAGCTGCG TGCTGCCTCC 
GTGGTCCAGC CCCAGCCCCT CGTGGTGGTG AAGGAGGAGA AGATCCACTC ACCCATCATC 
CGCAGCGAGC CCTTCAGCCC CTCGCTGCGG CCGGAGCCCC CCAAGCACCC GGAGAGCATC 
AAGGCCCCCG TCCACCTGCC CCAGCGGCCG GAAATGAAGC CTGTGGATGT CGGGAGGCCT 
GTGATCCGGC CCCCAGAGCA GAACGCACCG CCACCAGGGG CCCCTGACAA GGACAAACAG 
AAACAGGAGC CGAAGACTCC AGTTGCGCCC AAAAAGGACC TGAAAATCAA GAACATGGGC 
TCCTGGGCCA GCCTAGTGCA GAAGCATCCG ACCACCCCCT CCTCCACAGC CAAGTCATCC 
AGCGACAGCT TCGAGCAGTT CCGCCGCGCC GCTCGGGAGA AAGAGGAGCG TGAGAAGGCC 
CTGAAGGCTC AGGCCGAGCA CGCTGAGAAG GAGAAGGAGC GGCTGCGGCA GGAGCGCATG 
AGGAGCCGAG AGGACGAGGA TGCGCTGGAG CAGGCCCGGC GGGCCCATGA GGAGGCACGT 
CGGCGCCAGG AGCAGCAGCA GCAGCAGCGC CAGGAGCAAC AGCAGCAGCA GCAACAGCAA 
GCAGCTGCGG TGGCTGCCGC CGCCACCCCA CAGGCCCAGA GCTCCCAGCC CCAGTCCATG 
CTGGACCAGC AGAGGGAGTT GGCCCGGAAG CGGGAGCAGG AGCGAAGACG CCGGGAAGCC 
ATGGCAGCTA CCATTGACAT GAATTTCCAG AGTGATCTAT TGTCAATATT TGAAGAAAAT 
CTTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_490597.1
CDS	223..4311
Translation	MSAESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGTAKPGVSTVPNTTQASTPPQTQTPQPNPPPVQATPHPFPAVTPDLIVQTPVMTVVPPQPLQTPPPVPPQPQPPPAPAPQPVQSHPPIIAATPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLPPEPKTTKLGQRRESSRPVKPPKKDVPDSQQHPAPEKSSKVSEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLEAREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVAVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKRKEEVEENKKSKAKEPPPKKTKKNNSSNSNVSKKEPAPMKSKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESSSESSSSDSEDSETEMAPKSKKKGHPGREQKKHHHHHHQQMQQAPAPVPQQPPPPPQQPPPPPPPQQQQQPPPPPPPPSMPQQAAPAMKSSPPPFIATQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPARPPAVSPALTQTPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQLQQQPPPPPPPQPQPPPQQQHQPPPRPVHLQPMQFSTHIQQPPPPQGQQPPHPPPGQQPPPPQPAKPQQVIQHHHSPRHHKSDPYSTGHLREAPSPLMIHSPQMSQFQSLTHQSPPQQNVQPKKQELRAASVVQPQPLVVVKEEKIHSPIIRSEPFSPSLRPEPPKHPESIKAPVHLPQRPEMKPVDVGRPVIRPPEQNAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEQFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQRQEQQQQQQQQAAAVAAAATPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	NM_058243.2
Organism	Homo sapiens(human)
Definition	Homo sapiens bromodomain containing 4 (BRD4), transcript variant long, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058243.2

ORF Insert Sequence:

ATGTCTGCGG AGAGCGGCCC TGGGACGAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACTT CCCAAATGTC TACAACACAG GCCCAGGCCC AACCCCAGCC AGCCAACGCA 
GCCAGCACCA ACCCCCCGCC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATACCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCA 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATC 
ATTAAAACGC CTATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTACAAC 
AAGCCTGGAG ATGACATAGT CTTAATGGCA GAAGCTCTGG AAAAGCTCTT CTTGCAAAAA 
ATAAATGAGC TACCCACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGTGGGAGGA AAGAAACAGG GACAGCAAAA CCTGGCGTTT CCACGGTACC AAACACAACT 
CAAGCATCGA CTCCTCCGCA GACCCAGACC CCTCAGCCGA ATCCTCCTCC TGTGCAGGCC 
ACGCCTCACC CCTTCCCTGC CGTCACCCCG GACCTCATCG TCCAGACCCC TGTCATGACA 
GTGGTGCCTC CCCAGCCACT GCAGACGCCC CCGCCAGTGC CCCCCCAGCC ACAACCCCCA 
CCCGCTCCAG CTCCCCAGCC CGTACAGAGC CACCCACCCA TCATCGCGGC CACCCCACAG 
CCTGTGAAGA CAAAGAAGGG AGTGAAGAGG AAAGCAGACA CCACCACCCC CACCACCATT 
GACCCCATTC ACGAGCCACC CTCGCTGCCC CCGGAGCCCA AGACCACCAA GCTGGGCCAG 
CGGCGGGAGA GCAGCCGGCC TGTGAAACCT CCAAAGAAGG ACGTGCCCGA CTCTCAGCAG 
CACCCAGCAC CAGAGAAGAG CAGCAAGGTC TCGGAGCAGC TCAAGTGCTG CAGCGGCATC 
CTCAAGGAGA TGTTTGCCAA GAAGCACGCC GCCTACGCCT GGCCCTTCTA CAAGCCTGTG 
GACGTGGAGG CACTGGGCCT ACACGACTAC TGTGACATCA TCAAGCACCC CATGGACATG 
AGCACAATCA AGTCTAAACT GGAGGCCCGT GAGTACCGTG ATGCTCAGGA GTTTGGTGCT 
GACGTCCGAT TGATGTTCTC CAACTGCTAT AAGTACAACC CTCCTGACCA TGAGGTGGTG 
GCCATGGCCC GCAAGCTCCA GGATGTGTTC GAAATGCGCT TTGCCAAGAT GCCGGACGAG 
CCTGAGGAGC CAGTGGTGGC CGTGTCCTCC CCGGCAGTGC CCCCTCCCAC CAAGGTTGTG 
GCCCCGCCCT CATCCAGCGA CAGCAGCAGC GATAGCTCCT CGGACAGTGA CAGTTCGACT 
GATGACTCTG AGGAGGAGCG AGCCCAGCGG CTGGCTGAGC TCCAGGAGCA GCTCAAAGCC 
GTGCACGAGC AGCTTGCAGC CCTCTCTCAG CCCCAGCAGA ACAAACCAAA GAAAAAGGAG 
AAAGACAAGA AGGAAAAGAA AAAAGAAAAG CACAAAAGGA AAGAGGAAGT GGAAGAGAAT 
AAAAAAAGCA AAGCCAAGGA ACCTCCTCCT AAAAAGACGA AGAAAAATAA TAGCAGCAAC 
AGCAATGTGA GCAAGAAGGA GCCAGCGCCC ATGAAGAGCA AGCCCCCTCC CACGTATGAG 
TCGGAGGAAG AGGACAAGTG CAAGCCTATG TCCTATGAGG AGAAGCGGCA GCTCAGCTTG 
GACATCAACA AGCTCCCCGG CGAGAAGCTG GGCCGCGTGG TGCACATCAT CCAGTCACGG 
GAGCCCTCCC TGAAGAATTC CAACCCCGAC GAGATTGAAA TCGACTTTGA GACCCTGAAG 
CCGTCCACAC TGCGTGAGCT GGAGCGCTAT GTCACCTCCT GTTTGCGGAA GAAAAGGAAA 
CCTCAAGCTG AGAAAGTTGA TGTGATTGCC GGCTCCTCCA AGATGAAGGG CTTCTCGTCC 
TCAGAGTCGG AGAGCTCCAG TGAGTCCAGC TCCTCTGACA GCGAAGACTC CGAAACAGAG 
ATGGCTCCGA AGTCAAAAAA GAAGGGGCAC CCCGGGAGGG AGCAGAAGAA GCACCATCAT 
CACCACCATC AGCAGATGCA GCAGGCCCCG GCTCCTGTGC CCCAGCAGCC GCCCCCGCCT 
CCCCAGCAGC CCCCACCGCC TCCACCTCCG CAGCAGCAAC AGCAGCCGCC ACCCCCGCCT 
CCCCCACCCT CCATGCCGCA GCAGGCAGCC CCGGCGATGA AGTCCTCGCC CCCACCCTTC 
ATTGCCACCC AGGTGCCCGT CCTGGAGCCC CAGCTCCCAG GCAGCGTCTT TGACCCCATC 
GGCCACTTCA CCCAGCCCAT CCTGCACCTG CCGCAGCCTG AGCTGCCCCC TCACCTGCCC 
CAGCCGCCTG AGCACAGCAC TCCACCCCAT CTCAACCAGC ACGCAGTGGT CTCTCCTCCA 
GCTTTGCACA ACGCACTACC CCAGCAGCCA TCACGGCCCA GCAACCGAGC CGCTGCCCTG 
CCTCCCAAGC CCGCCCGGCC CCCAGCCGTG TCACCAGCCT TGACCCAAAC ACCCCTGCTC 
CCACAGCCCC CCATGGCCCA ACCCCCCCAA GTGCTGCTGG AGGATGAAGA GCCACCTGCC 
CCACCCCTCA CCTCCATGCA GATGCAGCTG TACCTGCAGC AGCTGCAGAA GGTGCAGCCC 
CCTACGCCGC TACTCCCTTC CGTGAAGGTG CAGTCCCAGC CCCCACCCCC CCTGCCGCCC 
CCACCCCACC CCTCTGTGCA GCAGCAGCTG CAGCAGCAGC CGCCACCACC CCCACCACCC 
CAGCCCCAGC CTCCACCCCA GCAGCAGCAT CAGCCCCCTC CACGGCCCGT GCACTTGCAG 
CCCATGCAGT TTTCCACCCA CATCCAACAG CCCCCGCCAC CCCAGGGCCA GCAGCCCCCC 
CATCCGCCCC CAGGCCAGCA GCCACCCCCG CCGCAGCCTG CCAAGCCTCA GCAAGTCATC 
CAGCACCACC ATTCACCCCG GCACCACAAG TCGGACCCCT ACTCAACCGG TCACCTCCGC 
GAAGCCCCCT CCCCGCTTAT GATACATTCC CCCCAGATGT CACAGTTCCA GAGCCTGACC 
CACCAGTCTC CACCCCAGCA AAACGTCCAG CCTAAGAAAC AGGAGCTGCG TGCTGCCTCC 
GTGGTCCAGC CCCAGCCCCT CGTGGTGGTG AAGGAGGAGA AGATCCACTC ACCCATCATC 
CGCAGCGAGC CCTTCAGCCC CTCGCTGCGG CCGGAGCCCC CCAAGCACCC GGAGAGCATC 
AAGGCCCCCG TCCACCTGCC CCAGCGGCCG GAAATGAAGC CTGTGGATGT CGGGAGGCCT 
GTGATCCGGC CCCCAGAGCA GAACGCACCG CCACCAGGGG CCCCTGACAA GGACAAACAG 
AAACAGGAGC CGAAGACTCC AGTTGCGCCC AAAAAGGACC TGAAAATCAA GAACATGGGC 
TCCTGGGCCA GCCTAGTGCA GAAGCATCCG ACCACCCCCT CCTCCACAGC CAAGTCATCC 
AGCGACAGCT TCGAGCAGTT CCGCCGCGCC GCTCGGGAGA AAGAGGAGCG TGAGAAGGCC 
CTGAAGGCTC AGGCCGAGCA CGCTGAGAAG GAGAAGGAGC GGCTGCGGCA GGAGCGCATG 
AGGAGCCGAG AGGACGAGGA TGCGCTGGAG CAGGCCCGGC GGGCCCATGA GGAGGCACGT 
CGGCGCCAGG AGCAGCAGCA GCAGCAGCGC CAGGAGCAAC AGCAGCAGCA GCAACAGCAA 
GCAGCTGCGG TGGCTGCCGC CGCCACCCCA CAGGCCCAGA GCTCCCAGCC CCAGTCCATG 
CTGGACCAGC AGAGGGAGTT GGCCCGGAAG CGGGAGCAGG AGCGAAGACG CCGGGAAGCC 
ATGGCAGCTA CCATTGACAT GAATTTCCAG AGTGATCTAT TGTCAATATT TGAAGAAAAT 
CTTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRD4 ( XM_011527854.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526156.1 Homo sapiens bromodomain containing 4 (BRD4), transcript variant X1, mRNA.

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CloneID	OHu27190
Clone ID Related Accession (Same CDS sequence)	XM_011527854.1 , XM_011527854.2 , NM_058243.2
Accession Version	XM_011527854.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4089bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	bromodomain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCGG AGAGCGGCCC TGGGACGAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACTT CCCAAATGTC TACAACACAG GCCCAGGCCC AACCCCAGCC AGCCAACGCA 
GCCAGCACCA ACCCCCCGCC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATACCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCA 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATC 
ATTAAAACGC CTATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTACAAC 
AAGCCTGGAG ATGACATAGT CTTAATGGCA GAAGCTCTGG AAAAGCTCTT CTTGCAAAAA 
ATAAATGAGC TACCCACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGTGGGAGGA AAGAAACAGG GACAGCAAAA CCTGGCGTTT CCACGGTACC AAACACAACT 
CAAGCATCGA CTCCTCCGCA GACCCAGACC CCTCAGCCGA ATCCTCCTCC TGTGCAGGCC 
ACGCCTCACC CCTTCCCTGC CGTCACCCCG GACCTCATCG TCCAGACCCC TGTCATGACA 
GTGGTGCCTC CCCAGCCACT GCAGACGCCC CCGCCAGTGC CCCCCCAGCC ACAACCCCCA 
CCCGCTCCAG CTCCCCAGCC CGTACAGAGC CACCCACCCA TCATCGCGGC CACCCCACAG 
CCTGTGAAGA CAAAGAAGGG AGTGAAGAGG AAAGCAGACA CCACCACCCC CACCACCATT 
GACCCCATTC ACGAGCCACC CTCGCTGCCC CCGGAGCCCA AGACCACCAA GCTGGGCCAG 
CGGCGGGAGA GCAGCCGGCC TGTGAAACCT CCAAAGAAGG ACGTGCCCGA CTCTCAGCAG 
CACCCAGCAC CAGAGAAGAG CAGCAAGGTC TCGGAGCAGC TCAAGTGCTG CAGCGGCATC 
CTCAAGGAGA TGTTTGCCAA GAAGCACGCC GCCTACGCCT GGCCCTTCTA CAAGCCTGTG 
GACGTGGAGG CACTGGGCCT ACACGACTAC TGTGACATCA TCAAGCACCC CATGGACATG 
AGCACAATCA AGTCTAAACT GGAGGCCCGT GAGTACCGTG ATGCTCAGGA GTTTGGTGCT 
GACGTCCGAT TGATGTTCTC CAACTGCTAT AAGTACAACC CTCCTGACCA TGAGGTGGTG 
GCCATGGCCC GCAAGCTCCA GGATGTGTTC GAAATGCGCT TTGCCAAGAT GCCGGACGAG 
CCTGAGGAGC CAGTGGTGGC CGTGTCCTCC CCGGCAGTGC CCCCTCCCAC CAAGGTTGTG 
GCCCCGCCCT CATCCAGCGA CAGCAGCAGC GATAGCTCCT CGGACAGTGA CAGTTCGACT 
GATGACTCTG AGGAGGAGCG AGCCCAGCGG CTGGCTGAGC TCCAGGAGCA GCTCAAAGCC 
GTGCACGAGC AGCTTGCAGC CCTCTCTCAG CCCCAGCAGA ACAAACCAAA GAAAAAGGAG 
AAAGACAAGA AGGAAAAGAA AAAAGAAAAG CACAAAAGGA AAGAGGAAGT GGAAGAGAAT 
AAAAAAAGCA AAGCCAAGGA ACCTCCTCCT AAAAAGACGA AGAAAAATAA TAGCAGCAAC 
AGCAATGTGA GCAAGAAGGA GCCAGCGCCC ATGAAGAGCA AGCCCCCTCC CACGTATGAG 
TCGGAGGAAG AGGACAAGTG CAAGCCTATG TCCTATGAGG AGAAGCGGCA GCTCAGCTTG 
GACATCAACA AGCTCCCCGG CGAGAAGCTG GGCCGCGTGG TGCACATCAT CCAGTCACGG 
GAGCCCTCCC TGAAGAATTC CAACCCCGAC GAGATTGAAA TCGACTTTGA GACCCTGAAG 
CCGTCCACAC TGCGTGAGCT GGAGCGCTAT GTCACCTCCT GTTTGCGGAA GAAAAGGAAA 
CCTCAAGCTG AGAAAGTTGA TGTGATTGCC GGCTCCTCCA AGATGAAGGG CTTCTCGTCC 
TCAGAGTCGG AGAGCTCCAG TGAGTCCAGC TCCTCTGACA GCGAAGACTC CGAAACAGAG 
ATGGCTCCGA AGTCAAAAAA GAAGGGGCAC CCCGGGAGGG AGCAGAAGAA GCACCATCAT 
CACCACCATC AGCAGATGCA GCAGGCCCCG GCTCCTGTGC CCCAGCAGCC GCCCCCGCCT 
CCCCAGCAGC CCCCACCGCC TCCACCTCCG CAGCAGCAAC AGCAGCCGCC ACCCCCGCCT 
CCCCCACCCT CCATGCCGCA GCAGGCAGCC CCGGCGATGA AGTCCTCGCC CCCACCCTTC 
ATTGCCACCC AGGTGCCCGT CCTGGAGCCC CAGCTCCCAG GCAGCGTCTT TGACCCCATC 
GGCCACTTCA CCCAGCCCAT CCTGCACCTG CCGCAGCCTG AGCTGCCCCC TCACCTGCCC 
CAGCCGCCTG AGCACAGCAC TCCACCCCAT CTCAACCAGC ACGCAGTGGT CTCTCCTCCA 
GCTTTGCACA ACGCACTACC CCAGCAGCCA TCACGGCCCA GCAACCGAGC CGCTGCCCTG 
CCTCCCAAGC CCGCCCGGCC CCCAGCCGTG TCACCAGCCT TGACCCAAAC ACCCCTGCTC 
CCACAGCCCC CCATGGCCCA ACCCCCCCAA GTGCTGCTGG AGGATGAAGA GCCACCTGCC 
CCACCCCTCA CCTCCATGCA GATGCAGCTG TACCTGCAGC AGCTGCAGAA GGTGCAGCCC 
CCTACGCCGC TACTCCCTTC CGTGAAGGTG CAGTCCCAGC CCCCACCCCC CCTGCCGCCC 
CCACCCCACC CCTCTGTGCA GCAGCAGCTG CAGCAGCAGC CGCCACCACC CCCACCACCC 
CAGCCCCAGC CTCCACCCCA GCAGCAGCAT CAGCCCCCTC CACGGCCCGT GCACTTGCAG 
CCCATGCAGT TTTCCACCCA CATCCAACAG CCCCCGCCAC CCCAGGGCCA GCAGCCCCCC 
CATCCGCCCC CAGGCCAGCA GCCACCCCCG CCGCAGCCTG CCAAGCCTCA GCAAGTCATC 
CAGCACCACC ATTCACCCCG GCACCACAAG TCGGACCCCT ACTCAACCGG TCACCTCCGC 
GAAGCCCCCT CCCCGCTTAT GATACATTCC CCCCAGATGT CACAGTTCCA GAGCCTGACC 
CACCAGTCTC CACCCCAGCA AAACGTCCAG CCTAAGAAAC AGGAGCTGCG TGCTGCCTCC 
GTGGTCCAGC CCCAGCCCCT CGTGGTGGTG AAGGAGGAGA AGATCCACTC ACCCATCATC 
CGCAGCGAGC CCTTCAGCCC CTCGCTGCGG CCGGAGCCCC CCAAGCACCC GGAGAGCATC 
AAGGCCCCCG TCCACCTGCC CCAGCGGCCG GAAATGAAGC CTGTGGATGT CGGGAGGCCT 
GTGATCCGGC CCCCAGAGCA GAACGCACCG CCACCAGGGG CCCCTGACAA GGACAAACAG 
AAACAGGAGC CGAAGACTCC AGTTGCGCCC AAAAAGGACC TGAAAATCAA GAACATGGGC 
TCCTGGGCCA GCCTAGTGCA GAAGCATCCG ACCACCCCCT CCTCCACAGC CAAGTCATCC 
AGCGACAGCT TCGAGCAGTT CCGCCGCGCC GCTCGGGAGA AAGAGGAGCG TGAGAAGGCC 
CTGAAGGCTC AGGCCGAGCA CGCTGAGAAG GAGAAGGAGC GGCTGCGGCA GGAGCGCATG 
AGGAGCCGAG AGGACGAGGA TGCGCTGGAG CAGGCCCGGC GGGCCCATGA GGAGGCACGT 
CGGCGCCAGG AGCAGCAGCA GCAGCAGCGC CAGGAGCAAC AGCAGCAGCA GCAACAGCAA 
GCAGCTGCGG TGGCTGCCGC CGCCACCCCA CAGGCCCAGA GCTCCCAGCC CCAGTCCATG 
CTGGACCAGC AGAGGGAGTT GGCCCGGAAG CGGGAGCAGG AGCGAAGACG CCGGGAAGCC 
ATGGCAGCTA CCATTGACAT GAATTTCCAG AGTGATCTAT TGTCAATATT TGAAGAAAAT 
CTTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526156.1
CDS	288..4376
Translation	MSAESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGTAKPGVSTVPNTTQASTPPQTQTPQPNPPPVQATPHPFPAVTPDLIVQTPVMTVVPPQPLQTPPPVPPQPQPPPAPAPQPVQSHPPIIAATPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLPPEPKTTKLGQRRESSRPVKPPKKDVPDSQQHPAPEKSSKVSEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLEAREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVAVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKRKEEVEENKKSKAKEPPPKKTKKNNSSNSNVSKKEPAPMKSKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESSSESSSSDSEDSETEMAPKSKKKGHPGREQKKHHHHHHQQMQQAPAPVPQQPPPPPQQPPPPPPPQQQQQPPPPPPPPSMPQQAAPAMKSSPPPFIATQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPARPPAVSPALTQTPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQLQQQPPPPPPPQPQPPPQQQHQPPPRPVHLQPMQFSTHIQQPPPPQGQQPPHPPPGQQPPPPQPAKPQQVIQHHHSPRHHKSDPYSTGHLREAPSPLMIHSPQMSQFQSLTHQSPPQQNVQPKKQELRAASVVQPQPLVVVKEEKIHSPIIRSEPFSPSLRPEPPKHPESIKAPVHLPQRPEMKPVDVGRPVIRPPEQNAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEQFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQRQEQQQQQQQQAAAVAAAATPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_011527854.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bromodomain containing 4 (BRD4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527854.1

ORF Insert Sequence:

ATGTCTGCGG AGAGCGGCCC TGGGACGAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACTT CCCAAATGTC TACAACACAG GCCCAGGCCC AACCCCAGCC AGCCAACGCA 
GCCAGCACCA ACCCCCCGCC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATACCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCA 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATC 
ATTAAAACGC CTATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTACAAC 
AAGCCTGGAG ATGACATAGT CTTAATGGCA GAAGCTCTGG AAAAGCTCTT CTTGCAAAAA 
ATAAATGAGC TACCCACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGTGGGAGGA AAGAAACAGG GACAGCAAAA CCTGGCGTTT CCACGGTACC AAACACAACT 
CAAGCATCGA CTCCTCCGCA GACCCAGACC CCTCAGCCGA ATCCTCCTCC TGTGCAGGCC 
ACGCCTCACC CCTTCCCTGC CGTCACCCCG GACCTCATCG TCCAGACCCC TGTCATGACA 
GTGGTGCCTC CCCAGCCACT GCAGACGCCC CCGCCAGTGC CCCCCCAGCC ACAACCCCCA 
CCCGCTCCAG CTCCCCAGCC CGTACAGAGC CACCCACCCA TCATCGCGGC CACCCCACAG 
CCTGTGAAGA CAAAGAAGGG AGTGAAGAGG AAAGCAGACA CCACCACCCC CACCACCATT 
GACCCCATTC ACGAGCCACC CTCGCTGCCC CCGGAGCCCA AGACCACCAA GCTGGGCCAG 
CGGCGGGAGA GCAGCCGGCC TGTGAAACCT CCAAAGAAGG ACGTGCCCGA CTCTCAGCAG 
CACCCAGCAC CAGAGAAGAG CAGCAAGGTC TCGGAGCAGC TCAAGTGCTG CAGCGGCATC 
CTCAAGGAGA TGTTTGCCAA GAAGCACGCC GCCTACGCCT GGCCCTTCTA CAAGCCTGTG 
GACGTGGAGG CACTGGGCCT ACACGACTAC TGTGACATCA TCAAGCACCC CATGGACATG 
AGCACAATCA AGTCTAAACT GGAGGCCCGT GAGTACCGTG ATGCTCAGGA GTTTGGTGCT 
GACGTCCGAT TGATGTTCTC CAACTGCTAT AAGTACAACC CTCCTGACCA TGAGGTGGTG 
GCCATGGCCC GCAAGCTCCA GGATGTGTTC GAAATGCGCT TTGCCAAGAT GCCGGACGAG 
CCTGAGGAGC CAGTGGTGGC CGTGTCCTCC CCGGCAGTGC CCCCTCCCAC CAAGGTTGTG 
GCCCCGCCCT CATCCAGCGA CAGCAGCAGC GATAGCTCCT CGGACAGTGA CAGTTCGACT 
GATGACTCTG AGGAGGAGCG AGCCCAGCGG CTGGCTGAGC TCCAGGAGCA GCTCAAAGCC 
GTGCACGAGC AGCTTGCAGC CCTCTCTCAG CCCCAGCAGA ACAAACCAAA GAAAAAGGAG 
AAAGACAAGA AGGAAAAGAA AAAAGAAAAG CACAAAAGGA AAGAGGAAGT GGAAGAGAAT 
AAAAAAAGCA AAGCCAAGGA ACCTCCTCCT AAAAAGACGA AGAAAAATAA TAGCAGCAAC 
AGCAATGTGA GCAAGAAGGA GCCAGCGCCC ATGAAGAGCA AGCCCCCTCC CACGTATGAG 
TCGGAGGAAG AGGACAAGTG CAAGCCTATG TCCTATGAGG AGAAGCGGCA GCTCAGCTTG 
GACATCAACA AGCTCCCCGG CGAGAAGCTG GGCCGCGTGG TGCACATCAT CCAGTCACGG 
GAGCCCTCCC TGAAGAATTC CAACCCCGAC GAGATTGAAA TCGACTTTGA GACCCTGAAG 
CCGTCCACAC TGCGTGAGCT GGAGCGCTAT GTCACCTCCT GTTTGCGGAA GAAAAGGAAA 
CCTCAAGCTG AGAAAGTTGA TGTGATTGCC GGCTCCTCCA AGATGAAGGG CTTCTCGTCC 
TCAGAGTCGG AGAGCTCCAG TGAGTCCAGC TCCTCTGACA GCGAAGACTC CGAAACAGAG 
ATGGCTCCGA AGTCAAAAAA GAAGGGGCAC CCCGGGAGGG AGCAGAAGAA GCACCATCAT 
CACCACCATC AGCAGATGCA GCAGGCCCCG GCTCCTGTGC CCCAGCAGCC GCCCCCGCCT 
CCCCAGCAGC CCCCACCGCC TCCACCTCCG CAGCAGCAAC AGCAGCCGCC ACCCCCGCCT 
CCCCCACCCT CCATGCCGCA GCAGGCAGCC CCGGCGATGA AGTCCTCGCC CCCACCCTTC 
ATTGCCACCC AGGTGCCCGT CCTGGAGCCC CAGCTCCCAG GCAGCGTCTT TGACCCCATC 
GGCCACTTCA CCCAGCCCAT CCTGCACCTG CCGCAGCCTG AGCTGCCCCC TCACCTGCCC 
CAGCCGCCTG AGCACAGCAC TCCACCCCAT CTCAACCAGC ACGCAGTGGT CTCTCCTCCA 
GCTTTGCACA ACGCACTACC CCAGCAGCCA TCACGGCCCA GCAACCGAGC CGCTGCCCTG 
CCTCCCAAGC CCGCCCGGCC CCCAGCCGTG TCACCAGCCT TGACCCAAAC ACCCCTGCTC 
CCACAGCCCC CCATGGCCCA ACCCCCCCAA GTGCTGCTGG AGGATGAAGA GCCACCTGCC 
CCACCCCTCA CCTCCATGCA GATGCAGCTG TACCTGCAGC AGCTGCAGAA GGTGCAGCCC 
CCTACGCCGC TACTCCCTTC CGTGAAGGTG CAGTCCCAGC CCCCACCCCC CCTGCCGCCC 
CCACCCCACC CCTCTGTGCA GCAGCAGCTG CAGCAGCAGC CGCCACCACC CCCACCACCC 
CAGCCCCAGC CTCCACCCCA GCAGCAGCAT CAGCCCCCTC CACGGCCCGT GCACTTGCAG 
CCCATGCAGT TTTCCACCCA CATCCAACAG CCCCCGCCAC CCCAGGGCCA GCAGCCCCCC 
CATCCGCCCC CAGGCCAGCA GCCACCCCCG CCGCAGCCTG CCAAGCCTCA GCAAGTCATC 
CAGCACCACC ATTCACCCCG GCACCACAAG TCGGACCCCT ACTCAACCGG TCACCTCCGC 
GAAGCCCCCT CCCCGCTTAT GATACATTCC CCCCAGATGT CACAGTTCCA GAGCCTGACC 
CACCAGTCTC CACCCCAGCA AAACGTCCAG CCTAAGAAAC AGGAGCTGCG TGCTGCCTCC 
GTGGTCCAGC CCCAGCCCCT CGTGGTGGTG AAGGAGGAGA AGATCCACTC ACCCATCATC 
CGCAGCGAGC CCTTCAGCCC CTCGCTGCGG CCGGAGCCCC CCAAGCACCC GGAGAGCATC 
AAGGCCCCCG TCCACCTGCC CCAGCGGCCG GAAATGAAGC CTGTGGATGT CGGGAGGCCT 
GTGATCCGGC CCCCAGAGCA GAACGCACCG CCACCAGGGG CCCCTGACAA GGACAAACAG 
AAACAGGAGC CGAAGACTCC AGTTGCGCCC AAAAAGGACC TGAAAATCAA GAACATGGGC 
TCCTGGGCCA GCCTAGTGCA GAAGCATCCG ACCACCCCCT CCTCCACAGC CAAGTCATCC 
AGCGACAGCT TCGAGCAGTT CCGCCGCGCC GCTCGGGAGA AAGAGGAGCG TGAGAAGGCC 
CTGAAGGCTC AGGCCGAGCA CGCTGAGAAG GAGAAGGAGC GGCTGCGGCA GGAGCGCATG 
AGGAGCCGAG AGGACGAGGA TGCGCTGGAG CAGGCCCGGC GGGCCCATGA GGAGGCACGT 
CGGCGCCAGG AGCAGCAGCA GCAGCAGCGC CAGGAGCAAC AGCAGCAGCA GCAACAGCAA 
GCAGCTGCGG TGGCTGCCGC CGCCACCCCA CAGGCCCAGA GCTCCCAGCC CCAGTCCATG 
CTGGACCAGC AGAGGGAGTT GGCCCGGAAG CGGGAGCAGG AGCGAAGACG CCGGGAAGCC 
ATGGCAGCTA CCATTGACAT GAATTTCCAG AGTGATCTAT TGTCAATATT TGAAGAAAAT 
CTTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRD4 ( XM_011527854.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526156.1 Homo sapiens bromodomain containing 4 (BRD4), transcript variant X1, mRNA.

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CloneID	OHu27190
Clone ID Related Accession (Same CDS sequence)	XM_011527854.1 , XM_011527854.2 , NM_058243.2
Accession Version	XM_011527854.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4089bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bromodomain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011527854.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCGG AGAGCGGCCC TGGGACGAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACTT CCCAAATGTC TACAACACAG GCCCAGGCCC AACCCCAGCC AGCCAACGCA 
GCCAGCACCA ACCCCCCGCC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATACCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCA 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATC 
ATTAAAACGC CTATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTACAAC 
AAGCCTGGAG ATGACATAGT CTTAATGGCA GAAGCTCTGG AAAAGCTCTT CTTGCAAAAA 
ATAAATGAGC TACCCACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGTGGGAGGA AAGAAACAGG GACAGCAAAA CCTGGCGTTT CCACGGTACC AAACACAACT 
CAAGCATCGA CTCCTCCGCA GACCCAGACC CCTCAGCCGA ATCCTCCTCC TGTGCAGGCC 
ACGCCTCACC CCTTCCCTGC CGTCACCCCG GACCTCATCG TCCAGACCCC TGTCATGACA 
GTGGTGCCTC CCCAGCCACT GCAGACGCCC CCGCCAGTGC CCCCCCAGCC ACAACCCCCA 
CCCGCTCCAG CTCCCCAGCC CGTACAGAGC CACCCACCCA TCATCGCGGC CACCCCACAG 
CCTGTGAAGA CAAAGAAGGG AGTGAAGAGG AAAGCAGACA CCACCACCCC CACCACCATT 
GACCCCATTC ACGAGCCACC CTCGCTGCCC CCGGAGCCCA AGACCACCAA GCTGGGCCAG 
CGGCGGGAGA GCAGCCGGCC TGTGAAACCT CCAAAGAAGG ACGTGCCCGA CTCTCAGCAG 
CACCCAGCAC CAGAGAAGAG CAGCAAGGTC TCGGAGCAGC TCAAGTGCTG CAGCGGCATC 
CTCAAGGAGA TGTTTGCCAA GAAGCACGCC GCCTACGCCT GGCCCTTCTA CAAGCCTGTG 
GACGTGGAGG CACTGGGCCT ACACGACTAC TGTGACATCA TCAAGCACCC CATGGACATG 
AGCACAATCA AGTCTAAACT GGAGGCCCGT GAGTACCGTG ATGCTCAGGA GTTTGGTGCT 
GACGTCCGAT TGATGTTCTC CAACTGCTAT AAGTACAACC CTCCTGACCA TGAGGTGGTG 
GCCATGGCCC GCAAGCTCCA GGATGTGTTC GAAATGCGCT TTGCCAAGAT GCCGGACGAG 
CCTGAGGAGC CAGTGGTGGC CGTGTCCTCC CCGGCAGTGC CCCCTCCCAC CAAGGTTGTG 
GCCCCGCCCT CATCCAGCGA CAGCAGCAGC GATAGCTCCT CGGACAGTGA CAGTTCGACT 
GATGACTCTG AGGAGGAGCG AGCCCAGCGG CTGGCTGAGC TCCAGGAGCA GCTCAAAGCC 
GTGCACGAGC AGCTTGCAGC CCTCTCTCAG CCCCAGCAGA ACAAACCAAA GAAAAAGGAG 
AAAGACAAGA AGGAAAAGAA AAAAGAAAAG CACAAAAGGA AAGAGGAAGT GGAAGAGAAT 
AAAAAAAGCA AAGCCAAGGA ACCTCCTCCT AAAAAGACGA AGAAAAATAA TAGCAGCAAC 
AGCAATGTGA GCAAGAAGGA GCCAGCGCCC ATGAAGAGCA AGCCCCCTCC CACGTATGAG 
TCGGAGGAAG AGGACAAGTG CAAGCCTATG TCCTATGAGG AGAAGCGGCA GCTCAGCTTG 
GACATCAACA AGCTCCCCGG CGAGAAGCTG GGCCGCGTGG TGCACATCAT CCAGTCACGG 
GAGCCCTCCC TGAAGAATTC CAACCCCGAC GAGATTGAAA TCGACTTTGA GACCCTGAAG 
CCGTCCACAC TGCGTGAGCT GGAGCGCTAT GTCACCTCCT GTTTGCGGAA GAAAAGGAAA 
CCTCAAGCTG AGAAAGTTGA TGTGATTGCC GGCTCCTCCA AGATGAAGGG CTTCTCGTCC 
TCAGAGTCGG AGAGCTCCAG TGAGTCCAGC TCCTCTGACA GCGAAGACTC CGAAACAGAG 
ATGGCTCCGA AGTCAAAAAA GAAGGGGCAC CCCGGGAGGG AGCAGAAGAA GCACCATCAT 
CACCACCATC AGCAGATGCA GCAGGCCCCG GCTCCTGTGC CCCAGCAGCC GCCCCCGCCT 
CCCCAGCAGC CCCCACCGCC TCCACCTCCG CAGCAGCAAC AGCAGCCGCC ACCCCCGCCT 
CCCCCACCCT CCATGCCGCA GCAGGCAGCC CCGGCGATGA AGTCCTCGCC CCCACCCTTC 
ATTGCCACCC AGGTGCCCGT CCTGGAGCCC CAGCTCCCAG GCAGCGTCTT TGACCCCATC 
GGCCACTTCA CCCAGCCCAT CCTGCACCTG CCGCAGCCTG AGCTGCCCCC TCACCTGCCC 
CAGCCGCCTG AGCACAGCAC TCCACCCCAT CTCAACCAGC ACGCAGTGGT CTCTCCTCCA 
GCTTTGCACA ACGCACTACC CCAGCAGCCA TCACGGCCCA GCAACCGAGC CGCTGCCCTG 
CCTCCCAAGC CCGCCCGGCC CCCAGCCGTG TCACCAGCCT TGACCCAAAC ACCCCTGCTC 
CCACAGCCCC CCATGGCCCA ACCCCCCCAA GTGCTGCTGG AGGATGAAGA GCCACCTGCC 
CCACCCCTCA CCTCCATGCA GATGCAGCTG TACCTGCAGC AGCTGCAGAA GGTGCAGCCC 
CCTACGCCGC TACTCCCTTC CGTGAAGGTG CAGTCCCAGC CCCCACCCCC CCTGCCGCCC 
CCACCCCACC CCTCTGTGCA GCAGCAGCTG CAGCAGCAGC CGCCACCACC CCCACCACCC 
CAGCCCCAGC CTCCACCCCA GCAGCAGCAT CAGCCCCCTC CACGGCCCGT GCACTTGCAG 
CCCATGCAGT TTTCCACCCA CATCCAACAG CCCCCGCCAC CCCAGGGCCA GCAGCCCCCC 
CATCCGCCCC CAGGCCAGCA GCCACCCCCG CCGCAGCCTG CCAAGCCTCA GCAAGTCATC 
CAGCACCACC ATTCACCCCG GCACCACAAG TCGGACCCCT ACTCAACCGG TCACCTCCGC 
GAAGCCCCCT CCCCGCTTAT GATACATTCC CCCCAGATGT CACAGTTCCA GAGCCTGACC 
CACCAGTCTC CACCCCAGCA AAACGTCCAG CCTAAGAAAC AGGAGCTGCG TGCTGCCTCC 
GTGGTCCAGC CCCAGCCCCT CGTGGTGGTG AAGGAGGAGA AGATCCACTC ACCCATCATC 
CGCAGCGAGC CCTTCAGCCC CTCGCTGCGG CCGGAGCCCC CCAAGCACCC GGAGAGCATC 
AAGGCCCCCG TCCACCTGCC CCAGCGGCCG GAAATGAAGC CTGTGGATGT CGGGAGGCCT 
GTGATCCGGC CCCCAGAGCA GAACGCACCG CCACCAGGGG CCCCTGACAA GGACAAACAG 
AAACAGGAGC CGAAGACTCC AGTTGCGCCC AAAAAGGACC TGAAAATCAA GAACATGGGC 
TCCTGGGCCA GCCTAGTGCA GAAGCATCCG ACCACCCCCT CCTCCACAGC CAAGTCATCC 
AGCGACAGCT TCGAGCAGTT CCGCCGCGCC GCTCGGGAGA AAGAGGAGCG TGAGAAGGCC 
CTGAAGGCTC AGGCCGAGCA CGCTGAGAAG GAGAAGGAGC GGCTGCGGCA GGAGCGCATG 
AGGAGCCGAG AGGACGAGGA TGCGCTGGAG CAGGCCCGGC GGGCCCATGA GGAGGCACGT 
CGGCGCCAGG AGCAGCAGCA GCAGCAGCGC CAGGAGCAAC AGCAGCAGCA GCAACAGCAA 
GCAGCTGCGG TGGCTGCCGC CGCCACCCCA CAGGCCCAGA GCTCCCAGCC CCAGTCCATG 
CTGGACCAGC AGAGGGAGTT GGCCCGGAAG CGGGAGCAGG AGCGAAGACG CCGGGAAGCC 
ATGGCAGCTA CCATTGACAT GAATTTCCAG AGTGATCTAT TGTCAATATT TGAAGAAAAT 
CTTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526156.1
CDS	72..4160
Translation	MSAESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGTAKPGVSTVPNTTQASTPPQTQTPQPNPPPVQATPHPFPAVTPDLIVQTPVMTVVPPQPLQTPPPVPPQPQPPPAPAPQPVQSHPPIIAATPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLPPEPKTTKLGQRRESSRPVKPPKKDVPDSQQHPAPEKSSKVSEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLEAREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVAVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKRKEEVEENKKSKAKEPPPKKTKKNNSSNSNVSKKEPAPMKSKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESSSESSSSDSEDSETEMAPKSKKKGHPGREQKKHHHHHHQQMQQAPAPVPQQPPPPPQQPPPPPPPQQQQQPPPPPPPPSMPQQAAPAMKSSPPPFIATQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPARPPAVSPALTQTPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQLQQQPPPPPPPQPQPPPQQQHQPPPRPVHLQPMQFSTHIQQPPPPQGQQPPHPPPGQQPPPPQPAKPQQVIQHHHSPRHHKSDPYSTGHLREAPSPLMIHSPQMSQFQSLTHQSPPQQNVQPKKQELRAASVVQPQPLVVVKEEKIHSPIIRSEPFSPSLRPEPPKHPESIKAPVHLPQRPEMKPVDVGRPVIRPPEQNAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEQFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQRQEQQQQQQQQAAAVAAAATPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_011527854.2
Organism	Homo sapiens(human)
Definition	Homo sapiens bromodomain containing 4 (BRD4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527854.2

ORF Insert Sequence:

ATGTCTGCGG AGAGCGGCCC TGGGACGAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACTT CCCAAATGTC TACAACACAG GCCCAGGCCC AACCCCAGCC AGCCAACGCA 
GCCAGCACCA ACCCCCCGCC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATACCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCA 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATC 
ATTAAAACGC CTATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTACAAC 
AAGCCTGGAG ATGACATAGT CTTAATGGCA GAAGCTCTGG AAAAGCTCTT CTTGCAAAAA 
ATAAATGAGC TACCCACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGTGGGAGGA AAGAAACAGG GACAGCAAAA CCTGGCGTTT CCACGGTACC AAACACAACT 
CAAGCATCGA CTCCTCCGCA GACCCAGACC CCTCAGCCGA ATCCTCCTCC TGTGCAGGCC 
ACGCCTCACC CCTTCCCTGC CGTCACCCCG GACCTCATCG TCCAGACCCC TGTCATGACA 
GTGGTGCCTC CCCAGCCACT GCAGACGCCC CCGCCAGTGC CCCCCCAGCC ACAACCCCCA 
CCCGCTCCAG CTCCCCAGCC CGTACAGAGC CACCCACCCA TCATCGCGGC CACCCCACAG 
CCTGTGAAGA CAAAGAAGGG AGTGAAGAGG AAAGCAGACA CCACCACCCC CACCACCATT 
GACCCCATTC ACGAGCCACC CTCGCTGCCC CCGGAGCCCA AGACCACCAA GCTGGGCCAG 
CGGCGGGAGA GCAGCCGGCC TGTGAAACCT CCAAAGAAGG ACGTGCCCGA CTCTCAGCAG 
CACCCAGCAC CAGAGAAGAG CAGCAAGGTC TCGGAGCAGC TCAAGTGCTG CAGCGGCATC 
CTCAAGGAGA TGTTTGCCAA GAAGCACGCC GCCTACGCCT GGCCCTTCTA CAAGCCTGTG 
GACGTGGAGG CACTGGGCCT ACACGACTAC TGTGACATCA TCAAGCACCC CATGGACATG 
AGCACAATCA AGTCTAAACT GGAGGCCCGT GAGTACCGTG ATGCTCAGGA GTTTGGTGCT 
GACGTCCGAT TGATGTTCTC CAACTGCTAT AAGTACAACC CTCCTGACCA TGAGGTGGTG 
GCCATGGCCC GCAAGCTCCA GGATGTGTTC GAAATGCGCT TTGCCAAGAT GCCGGACGAG 
CCTGAGGAGC CAGTGGTGGC CGTGTCCTCC CCGGCAGTGC CCCCTCCCAC CAAGGTTGTG 
GCCCCGCCCT CATCCAGCGA CAGCAGCAGC GATAGCTCCT CGGACAGTGA CAGTTCGACT 
GATGACTCTG AGGAGGAGCG AGCCCAGCGG CTGGCTGAGC TCCAGGAGCA GCTCAAAGCC 
GTGCACGAGC AGCTTGCAGC CCTCTCTCAG CCCCAGCAGA ACAAACCAAA GAAAAAGGAG 
AAAGACAAGA AGGAAAAGAA AAAAGAAAAG CACAAAAGGA AAGAGGAAGT GGAAGAGAAT 
AAAAAAAGCA AAGCCAAGGA ACCTCCTCCT AAAAAGACGA AGAAAAATAA TAGCAGCAAC 
AGCAATGTGA GCAAGAAGGA GCCAGCGCCC ATGAAGAGCA AGCCCCCTCC CACGTATGAG 
TCGGAGGAAG AGGACAAGTG CAAGCCTATG TCCTATGAGG AGAAGCGGCA GCTCAGCTTG 
GACATCAACA AGCTCCCCGG CGAGAAGCTG GGCCGCGTGG TGCACATCAT CCAGTCACGG 
GAGCCCTCCC TGAAGAATTC CAACCCCGAC GAGATTGAAA TCGACTTTGA GACCCTGAAG 
CCGTCCACAC TGCGTGAGCT GGAGCGCTAT GTCACCTCCT GTTTGCGGAA GAAAAGGAAA 
CCTCAAGCTG AGAAAGTTGA TGTGATTGCC GGCTCCTCCA AGATGAAGGG CTTCTCGTCC 
TCAGAGTCGG AGAGCTCCAG TGAGTCCAGC TCCTCTGACA GCGAAGACTC CGAAACAGAG 
ATGGCTCCGA AGTCAAAAAA GAAGGGGCAC CCCGGGAGGG AGCAGAAGAA GCACCATCAT 
CACCACCATC AGCAGATGCA GCAGGCCCCG GCTCCTGTGC CCCAGCAGCC GCCCCCGCCT 
CCCCAGCAGC CCCCACCGCC TCCACCTCCG CAGCAGCAAC AGCAGCCGCC ACCCCCGCCT 
CCCCCACCCT CCATGCCGCA GCAGGCAGCC CCGGCGATGA AGTCCTCGCC CCCACCCTTC 
ATTGCCACCC AGGTGCCCGT CCTGGAGCCC CAGCTCCCAG GCAGCGTCTT TGACCCCATC 
GGCCACTTCA CCCAGCCCAT CCTGCACCTG CCGCAGCCTG AGCTGCCCCC TCACCTGCCC 
CAGCCGCCTG AGCACAGCAC TCCACCCCAT CTCAACCAGC ACGCAGTGGT CTCTCCTCCA 
GCTTTGCACA ACGCACTACC CCAGCAGCCA TCACGGCCCA GCAACCGAGC CGCTGCCCTG 
CCTCCCAAGC CCGCCCGGCC CCCAGCCGTG TCACCAGCCT TGACCCAAAC ACCCCTGCTC 
CCACAGCCCC CCATGGCCCA ACCCCCCCAA GTGCTGCTGG AGGATGAAGA GCCACCTGCC 
CCACCCCTCA CCTCCATGCA GATGCAGCTG TACCTGCAGC AGCTGCAGAA GGTGCAGCCC 
CCTACGCCGC TACTCCCTTC CGTGAAGGTG CAGTCCCAGC CCCCACCCCC CCTGCCGCCC 
CCACCCCACC CCTCTGTGCA GCAGCAGCTG CAGCAGCAGC CGCCACCACC CCCACCACCC 
CAGCCCCAGC CTCCACCCCA GCAGCAGCAT CAGCCCCCTC CACGGCCCGT GCACTTGCAG 
CCCATGCAGT TTTCCACCCA CATCCAACAG CCCCCGCCAC CCCAGGGCCA GCAGCCCCCC 
CATCCGCCCC CAGGCCAGCA GCCACCCCCG CCGCAGCCTG CCAAGCCTCA GCAAGTCATC 
CAGCACCACC ATTCACCCCG GCACCACAAG TCGGACCCCT ACTCAACCGG TCACCTCCGC 
GAAGCCCCCT CCCCGCTTAT GATACATTCC CCCCAGATGT CACAGTTCCA GAGCCTGACC 
CACCAGTCTC CACCCCAGCA AAACGTCCAG CCTAAGAAAC AGGAGCTGCG TGCTGCCTCC 
GTGGTCCAGC CCCAGCCCCT CGTGGTGGTG AAGGAGGAGA AGATCCACTC ACCCATCATC 
CGCAGCGAGC CCTTCAGCCC CTCGCTGCGG CCGGAGCCCC CCAAGCACCC GGAGAGCATC 
AAGGCCCCCG TCCACCTGCC CCAGCGGCCG GAAATGAAGC CTGTGGATGT CGGGAGGCCT 
GTGATCCGGC CCCCAGAGCA GAACGCACCG CCACCAGGGG CCCCTGACAA GGACAAACAG 
AAACAGGAGC CGAAGACTCC AGTTGCGCCC AAAAAGGACC TGAAAATCAA GAACATGGGC 
TCCTGGGCCA GCCTAGTGCA GAAGCATCCG ACCACCCCCT CCTCCACAGC CAAGTCATCC 
AGCGACAGCT TCGAGCAGTT CCGCCGCGCC GCTCGGGAGA AAGAGGAGCG TGAGAAGGCC 
CTGAAGGCTC AGGCCGAGCA CGCTGAGAAG GAGAAGGAGC GGCTGCGGCA GGAGCGCATG 
AGGAGCCGAG AGGACGAGGA TGCGCTGGAG CAGGCCCGGC GGGCCCATGA GGAGGCACGT 
CGGCGCCAGG AGCAGCAGCA GCAGCAGCGC CAGGAGCAAC AGCAGCAGCA GCAACAGCAA 
GCAGCTGCGG TGGCTGCCGC CGCCACCCCA CAGGCCCAGA GCTCCCAGCC CCAGTCCATG 
CTGGACCAGC AGAGGGAGTT GGCCCGGAAG CGGGAGCAGG AGCGAAGACG CCGGGAAGCC 
ATGGCAGCTA CCATTGACAT GAATTTCCAG AGTGATCTAT TGTCAATATT TGAAGAAAAT 
CTTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRD4 cDNA ORF clone, Homo sapiens(human)

Summary

Reference Sequences (RefSeq)

Pathways from BioSystems

Homologies

Gene Ontology

Reference Sequences (Refseq)

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

PubMed

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