CASP14 cDNA ORF clone, Homo sapiens(human)
| Gene Symbol | CASP14 |
| Entrez Gene ID | 23581 |
| Full Name | caspase 14 |
| Synonyms | ARCI12 |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Homo sapiens(human) |
| Genome | |
| Summary | This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This caspase has been shown to be processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier. [provided by RefSeq, Jul 2008]. |
| Disorder MIM: | |
| Disorder Html: | Ichthyosis, congenital, autosomal recessive 12, 617320 (3) |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| XM_011527861.1 | XP_011526163.1 | caspase-14 isoform X1 |
| NM_012114.3 | NP_036246.1 | caspase-14 precursor |
| NM_012114.2 | NP_036246.1 | caspase-14 precursor |
| Pan troglodytes (chimpanzee) | CASP14 | XP_001159009.2 |
| Canis lupus familiaris (dog) | CASP14 | XP_853064.2 |
| Mus musculus (house mouse) | Casp14 | NP_033939.1 |
| Rattus norvegicus (Norway rat) | Casp14 | NP_001178705.1 |
| Bos taurus (cattle) | CASP14 | NP_001192772.1 |
| Homo sapiens (human) | CASP14 | NP_036246.1 |
| CASP14 | XP_001111598.1 |
Related articles in PubMed
Caspase14 expression is associated with triple negative phenotypes and cancer stem cell marker expression in breast cancer patients.
Handa T, Katayama A, Yokobori T, Yamane A, Horiguchi J, Kawabata-Iwakawa R, Rokudai S, Bao P, Gombodorj N, Altan B, Kaira K, Asao T, Kuwano H, Nishiyama M, Oyama T
Journal of surgical oncology116(6)706-715(2017 Nov)
S100A7 acts as a dual regulator in promoting proliferation and suppressing squamous differentiation through GATA-3/caspase-14 pathway in A431 cells.
Li T, Qi Z, Kong F, Li Y, Wang R, Zhang W, Shang Y, Huang L, He D, Xiao X
Experimental dermatology24(5)342-8(2015 May)
Pyrrolidone carboxylic acid levels or caspase-14 expression in the corneocytes of lesional skin correlates with clinical severity, skin barrier function and lesional inflammation in atopic dermatitis.
Jung M, Choi J, Lee SA, Kim H, Hwang J, Choi EH
Journal of dermatological science76(3)231-9(2014 Dec)
Mesotrypsin and caspase-14 participate in prosaposin processing: potential relevance to epidermal permeability barrier formation.
Yamamoto-Tanaka M, Motoyama A, Miyai M, Matsunaga Y, Matsuda J, Tsuboi R, Hibino T
The Journal of biological chemistry289(29)20026-38(2014 Jul)
Caspase-14 expression impairs retinal pigment epithelium barrier function: potential role in diabetic macular edema.
Beasley S, El-Sherbiny M, Megyerdi S, El-Shafey S, Choksi K, Kaddour-Djebbar I, Sheibani N, Hsu S, Al-Shabrawey M
BioMed research international2014417986(2014)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
High CASP14 expression is a marker of breast cancer aggressiveness in association with proliferation, TNBC phenotype, and cancer stemness.
Title: Caspase14 expression is associated with triple negative phenotypes and cancer stem cell marker expression in breast cancer patients.
overexpression of S100A7 in A431 skin squamous carcinoma cells significantly promoted cell proliferation in vitro and tumor growth in vivo, whereas it suppressed the expression of GATA-3 and caspase-14
Title: S100A7 acts as a dual regulator in promoting proliferation and suppressing squamous differentiation through GATA-3/caspase-14 pathway in A431 cells.
Caspase-14 was decreased in inflammatory lesions compared to non-lesion in atopic dermatitis. The amount of caspase-14 in the lesions correlated with clinical severity as determined by eczema area and severity index score and the skin barrier functions.
Title: Pyrrolidone carboxylic acid levels or caspase-14 expression in the corneocytes of lesional skin correlates with clinical severity, skin barrier function and lesional inflammation in atopic dermatitis.
Mesotrypsin generated saposins A-D from prosaposin, and mature caspase-14 contributed to this process by activating mesotrypsinogen to mesotrypsin. Knockdown of these proteases markedly down-regulated saposin A synthesis in skin equivalent models.
Title: Mesotrypsin and caspase-14 participate in prosaposin processing: potential relevance to epidermal permeability barrier formation.
caspase-14 contributes to retinal pigment epithelium cell barrier disruption under hyperglycemic conditions.
Title: Caspase-14 expression impairs retinal pigment epithelium barrier function: potential role in diabetic macular edema.
The following CASP14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CASP14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OHu12493 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_012114.3 , NM_012114.2 | |
| Accession Version | NM_012114.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 729bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-23 | |
| Organism | Homo sapiens(human) | |
| Product | caspase-14 precursor | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK313992.1, AC004699.1 and BM840054.1. On Nov 23, 2018 this sequence version replaced NM_012114.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU502748.1, AK313992.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145893, SAMEA2147596 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000427043.4/ ENSP00000393417.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. | |
1 | ATGAGCAATC CGCGGTCTTT GGAAGAGGAG AAATATGATA TGTCAGGTGC CCGCCTGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_036246.1 |
| CDS | 213..941 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_012114.3 |
ORF Insert Sequence:
1 | ATGAGCAATC CGCGGTCTTT GGAAGAGGAG AAATATGATA TGTCAGGTGC CCGCCTGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu12493 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_012114.3 , NM_012114.2 | |
| Accession Version | NM_012114.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 729bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-12-05 | |
| Organism | Homo sapiens(human) | |
| Product | caspase-14 precursor | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK313992.1, AC004699.1 and BM840054.1. On Nov 24, 2010 this sequence version replaced NM_012114.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU502748.1, AK313992.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145893, SAMEA2147596 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGAGCAATC CGCGGTCTTT GGAAGAGGAG AAATATGATA TGTCAGGTGC CCGCCTGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_036246.1 |
| CDS | 213..941 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_012114.2 |
ORF Insert Sequence:
1 | ATGAGCAATC CGCGGTCTTT GGAAGAGGAG AAATATGATA TGTCAGGTGC CCGCCTGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu66337 | |
| Clone ID Related Accession (Same CDS sequence) | XM_011527861.1 | |
| Accession Version | XM_011527861.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 693bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-05 | |
| Organism | Homo sapiens(human) | |
| Product | caspase-14 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGCAATC CGCGGTCTTT GGAAGAGGAG AAATATGATA TGTCAGGTGC CCGCCTGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_011526163.1 |
| CDS | 213..905 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_011527861.1 |
ORF Insert Sequence:
1 | ATGAGCAATC CGCGGTCTTT GGAAGAGGAG AAATATGATA TGTCAGGTGC CCGCCTGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
Caspase14 expression is associated with triple negative phenotypes and cancer stem cell marker expression in breast cancer patients. |
|
S100A7 acts as a dual regulator in promoting proliferation and suppressing squamous differentiation through GATA-3/caspase-14 pathway in A431 cells. |
|
Pyrrolidone carboxylic acid levels or caspase-14 expression in the corneocytes of lesional skin correlates with clinical severity, skin barrier function and lesional inflammation in atopic dermatitis. |
|
Mesotrypsin and caspase-14 participate in prosaposin processing: potential relevance to epidermal permeability barrier formation. |
|
Caspase-14 expression impairs retinal pigment epithelium barrier function: potential role in diabetic macular edema. |