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LEMD3 cDNA ORF clone, Homo sapiens (human)

Gene Symbol LEMD3
Entrez Gene ID 23592
Full Name LEM domain containing 3
Synonyms MAN1
General protein information
Preferred Names
inner nuclear membrane protein Man1
Names
inner nuclear membrane protein Man1
LEM domain-containing protein 3
integral inner nuclear membrane protein
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12q14

Summary This locus encodes a LEM domain-containing protein. The encoded protein functions to antagonize transforming growth factor-beta signaling at the inner nuclear membrane. Two transcript variants encoding different isoforms have been found for this gene. Mutations in this gene have been associated with osteopoikilosis, Buschke-Ollendorff syndrome and melorheostosis.[provided by RefSeq, Nov 2009]. lac of sum
Disorder MIM:

607844

Disorder Html: Osteopoikilosis, 166700 (3); Buschke-Ollendorff syndrome, 166700 (3);

mRNA and Protein(s)

mRNA Protein Name
NM_014319 NP_055134 inner nuclear membrane protein Man1 isoform 1
NM_001167614 NP_001161086 inner nuclear membrane protein Man1 isoform 2



Homo sapiens (human) LEMD3 NP_055134.2
Pan troglodytes (chimpanzee) LEMD3 XP_509197.2
Macaca mulatta (Rhesus monkey) LEMD3 XP_001116983.1
Canis lupus familiaris (dog) LEMD3 XP_005625712.1
Bos taurus (cattle) LEMD3 NP_001179628.1
Mus musculus (house mouse) Lemd3 NP_001074662.2
Rattus norvegicus (Norway rat) Lemd3 NP_001177929.1
Gallus gallus (chicken) LEMD3 XP_416073.3
Danio rerio (zebrafish) lemd3 NP_001038329.1
Xenopus (Silurana) tropicalis (western clawed frog) lemd3 NP_001136130.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LEMD3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LEMD3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu19032 NM_014319 Homo sapiens LEM domain containing 3 (LEMD3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $643.30
$919.00
OHu04065 NM_001167614 Homo sapiens LEM domain containing 3 (LEMD3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $643.30
$919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19032
Clone ID Related Accession (Same CDS sequence) NM_014319
Accession Version NM_014319.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product inner nuclear membrane protein Man1 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK312473.1, AF112299.2 and BX390128.2. This sequence is a reference standard in the RefSeqGene project. On Nov 10, 2009 this sequence version replaced gi:36287116. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF112299.2, AK024954.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGCGGCGG CAGCAGCTTC GGCGCCTCAG CAGCTCTCGG ATGAGGAGCT TTTCTCTCAG 
CTCCGCCGTT ACGGCCTGTC TCCCGGACCA GTGACGGAGA GCACCCGCCC GGTCTACCTC
AAGAAGCTGA AGAAGCTTCG AGAGGAAGAG CAGCAACAGC ACCGGTCAGG GGGCCGCGGC
AACAAGACGC GGAACAGTAA TAACAATAAC ACGGCAGCCG CCACGGTCGC AGCCGCGGGA
CCAGCGGCGG CGGCGGCCGC GGGGATGGGG GTCCGGCCGG TCTCGGGCGA CCTCTCCTAC
TTACGGACTC CTGGGGGCCT GTGCCGAATC TCGGCCTCTG GCCCAGAGAG CCTCCTGGGA
GGGCCCGGGG GCGCCTCCGC CGCCCCCGCG GCTGGCAGCA AAGTGCTGCT GGGCTTCAGC
TCGGACGAGT CGGACGTGGA GGCCAGTCCC CGGGACCAGG CCGGCGGCGG CGGGAGGAAA
GACCGGGCTT CGCTCCAGTA CCGCGGGCTC AAAGCGCCGC CGGCGCCCCT GGCCGCCAGC
GAGGTGACTA ACAGCAACTC TGCAGAGCGA AGGAAGCCCC ACTCGTGGTG GGGGGCCAGG
AGGCCGGCGG GCCCCGAGCT GCAGACCCCG CCGGGGAAAG ATGGAGCAGT GGAGGACGAG
GAAGGGGAGG GAGAGGACGG TGAGGAGAGG GACCCGGAGA CCGAGGAGCC GCTCTGGGCG
AGCCGGACCG TGAATGGCAG CCGGCTTGTC CCCTACAGCT GCCGGGAAAA CTATTCGGAC
TCAGAGGAAG AGGACGACGA CGACGTGGCC TCCAGCAGAC AGGTATTAAA GGACGACTCC
CTTTCCCGGC ATCGGCCCAG ACGAACCCAT AGTAAGCCTC TCCCCCCGCT GACTGCTAAA
TCGGCCGGCG GCAGGCTGGA GACTTCAGTT CAGGGAGGGG GAGGACTCGC GATGAATGAC
AGGGCGGCGG CTGCCGGGAG TCTAGACAGG AGCCGAAACC TCGAAGAGGC GGCGGCCGCG
GAGCAGGGAG GAGGGTGTGA TCAAGTGGAC TCCAGCCCCG TTCCTAGATA CCGTGTTAAC
GCTAAGAAAC TGACCCCTCT CCTGCCCCCG CCACTTACTG ACATGGACTC AACCTTGGAT
TCGTCAACAG GCTCCCTTCT GAAAACCAAT AATCATATTG GCGGTGGGGC CTTCAGTGTG
GACTCCCCCA GGATTTATTC TAACAGTCTC CCTCCCAGTG CGGCGGTGGC CGCCTCTAGT
TCACTCAGGA TCAATCACGC CAATCATACG GGCTCCAATC ATACCTACCT GAAAAACACA
TACAACAAAC CGAAGCTTTC CGAACCCGAA GAGGAACTTC TCCAGCAATT TAAACGGGAG
GAGGTGTCCC CAACAGGGAG TTTCAGTGCC CACTACTTGT CGATGTTTCT CTTAACTGCT
GCCTGCTTAT TTTTCCTAAT ACTGGGACTG ACTTACCTAG GAATGAGAGG GACAGGAGTA
TCTGAGGATG GAGAACTCAG CATAGAAAAC CCCTTTGGTG AAACATTTGG AAAAATACAA
GAAAGTGAAA AAACTCTTAT GATGAACACA TTATATAAGC TTCATGATCG ATTGGCACAG
CTTGCAGGAG ATCATGAATG TGGCAGTTCT AGTCAAAGAA CGCTTTCTGT TCAAGAGGCA
GCTGCGTATT TAAAAGATTT AGGTCCTGAA TATGAAGGTA TATTTAACAC TTCATTGCAG
TGGATCTTAG AAAATGGAAA AGATGTTGGA ATAAGGTGTG TTGGTTTTGG CCCTGAGGAA
GAATTGACAA ATATAACTGA TGTGCAGTTT TTACAGTCCA CAAGACCACT GATGTCTTTT
TGGTGTCGTT TTCGACGTGC TTTTGTTACT GTAACTCACA GATTATTGTT GTTATGCTTA
GGTGTAGTGA TGGTTTGTGT CGTTCTGCGT TACATGAAAT ATCGATGGAC AAAAGAAGAG
GAGGAAACAA GGCAGATGTA TGATATGGTG GTAAAGATTA TAGATGTTTT ACGAAGTCAT
AATGAAGCCT GCCAGGAAAA CAAAGATTTA CAACCTTACA TGCCTATTCC ACATGTACGC
GATTCCTTAA TACAGCCTCA TGACAGGAAA AAAATGAAGA AAGTCTGGGA TAGAGCTGTT
GACTTCCTTG CTGCTAATGA GTCTAGAGTT CGCACGGAAA CACGAAGAAT AGGTGGTGCA
GATTTTCTGG TTTGGCGGTG GATCCAGCCT TCTGCATCCT GTGACAAAAT ATTAGTTATA
CCTTCTAAAG TATGGCAAGG TCAAGCATTT CATTTAGATA GAAGAAATTC ACCACCAAAT
AGTTTGACAC CGTGTCTAAA GATTCGGAAT ATGTTTGATC CCGTTATGGA AATAGGGGAT
CAGTGGCATT TGGCAATTCA AGAAGCAATT TTAGAAAAAT GCAGTGATAA TGATGGCATT
GTTCACATTG CAGTAGACAA AAATTCACGT GAGGGTTGTG TATATGTTAA ATGTCTGTCT
CCAGAATATG CTGGAAAGGC TTTTAAAGCA TTGCATGGCT CTTGGTTTGA TGGGAAATTG
GTTACAGTAA AATATTTACG ACTAGATAGA TACCACCATC GCTTTCCCCA GGCTCTCACT
TCCAACACTC CATTGAAGCC ATCAAATAAA CATATGAACT CCATGTCTCA TCTTCGTCTT
CGGACTGGCC TAACCAATTC TCAAGGAAGT TCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_055134.2
CDS27..2762
Misc Feature(1)30..32(+)
Misc Feature(2)51..179(+)
Misc Feature(3)105..152(+)
Misc Feature(4)444..446(+)
Misc Feature(5)447..449(+)
Misc Feature(6)456..458(+)
Misc Feature(7)579..581(+)
Misc Feature(8)585..587(+)
Misc Feature(9)801..803(+)
Misc Feature(10)807..809(+)
Misc Feature(11)864..866(+)
Misc Feature(12)1080..1082(+)
Misc Feature(13)1119..1121(+)
Misc Feature(14)1230..1232(+)
Misc Feature(15)1449..1511(+)
Misc Feature(16)1452..2279(+)
Misc Feature(17)1908..1970(+)
Misc Feature(18)2121..2759(+)
Misc Feature(19)2355..2357(+)
Misc Feature(20)2379..2654(+)
Misc Feature(21)2673..2675(+)
Misc Feature(22)2757..2759(+)
Exon (1)1..1548
Gene:LEMD3
Gene Synonym:
Exon (2)1549..1586
Gene:LEMD3
Gene Synonym:
Exon (3)1587..1653
Gene:LEMD3
Gene Synonym:
Exon (4)1654..1721
Gene:LEMD3
Gene Synonym:
Exon (5)1722..1801
Gene:LEMD3
Gene Synonym:
Exon (6)1802..1947
Gene:LEMD3
Gene Synonym:
Exon (7)1948..2049
Gene:LEMD3
Gene Synonym:
Exon (8)2050..2152
Gene:LEMD3
Gene Synonym:
Exon (9)2153..2331
Gene:LEMD3
Gene Synonym:
Exon (10)2332..2413
Gene:LEMD3
Gene Synonym:
Exon (11)2414..2519
Gene:LEMD3
Gene Synonym:
Exon (12)2520..2598
Gene:LEMD3
Gene Synonym:
Exon (13)2599..4798
Gene:LEMD3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_014319
Organism Homo sapiens (human)
Definition Homo sapiens LEM domain containing 3 (LEMD3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_014319

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGCGGCGG CAGCAGCTTC GGCGCCTCAG CAGCTCTCGG ATGAGGAGCT TTTCTCTCAG 
CTCCGCCGTT ACGGCCTGTC TCCCGGACCA GTGACGGAGA GCACCCGCCC GGTCTACCTC
AAGAAGCTGA AGAAGCTTCG AGAGGAAGAG CAGCAACAGC ACCGGTCAGG GGGCCGCGGC
AACAAGACGC GGAACAGTAA TAACAATAAC ACGGCAGCCG CCACGGTCGC AGCCGCGGGA
CCAGCGGCGG CGGCGGCCGC GGGGATGGGG GTCCGGCCGG TCTCGGGCGA CCTCTCCTAC
TTACGGACTC CTGGGGGCCT GTGCCGAATC TCGGCCTCTG GCCCAGAGAG CCTCCTGGGA
GGGCCCGGGG GCGCCTCCGC CGCCCCCGCG GCTGGCAGCA AAGTGCTGCT GGGCTTCAGC
TCGGACGAGT CGGACGTGGA GGCCAGTCCC CGGGACCAGG CCGGCGGCGG CGGGAGGAAA
GACCGGGCTT CGCTCCAGTA CCGCGGGCTC AAAGCGCCGC CGGCGCCCCT GGCCGCCAGC
GAGGTGACTA ACAGCAACTC TGCAGAGCGA AGGAAGCCCC ACTCGTGGTG GGGGGCCAGG
AGGCCGGCGG GCCCCGAGCT GCAGACCCCG CCGGGGAAAG ATGGAGCAGT GGAGGACGAG
GAAGGGGAGG GAGAGGACGG TGAGGAGAGG GACCCGGAGA CCGAGGAGCC GCTCTGGGCG
AGCCGGACCG TGAATGGCAG CCGGCTTGTC CCCTACAGCT GCCGGGAAAA CTATTCGGAC
TCAGAGGAAG AGGACGACGA CGACGTGGCC TCCAGCAGAC AGGTATTAAA GGACGACTCC
CTTTCCCGGC ATCGGCCCAG ACGAACCCAT AGTAAGCCTC TCCCCCCGCT GACTGCTAAA
TCGGCCGGCG GCAGGCTGGA GACTTCAGTT CAGGGAGGGG GAGGACTCGC GATGAATGAC
AGGGCGGCGG CTGCCGGGAG TCTAGACAGG AGCCGAAACC TCGAAGAGGC GGCGGCCGCG
GAGCAGGGAG GAGGGTGTGA TCAAGTGGAC TCCAGCCCCG TTCCTAGATA CCGTGTTAAC
GCTAAGAAAC TGACCCCTCT CCTGCCCCCG CCACTTACTG ACATGGACTC AACCTTGGAT
TCGTCAACAG GCTCCCTTCT GAAAACCAAT AATCATATTG GCGGTGGGGC CTTCAGTGTG
GACTCCCCCA GGATTTATTC TAACAGTCTC CCTCCCAGTG CGGCGGTGGC CGCCTCTAGT
TCACTCAGGA TCAATCACGC CAATCATACG GGCTCCAATC ATACCTACCT GAAAAACACA
TACAACAAAC CGAAGCTTTC CGAACCCGAA GAGGAACTTC TCCAGCAATT TAAACGGGAG
GAGGTGTCCC CAACAGGGAG TTTCAGTGCC CACTACTTGT CGATGTTTCT CTTAACTGCT
GCCTGCTTAT TTTTCCTAAT ACTGGGACTG ACTTACCTAG GAATGAGAGG GACAGGAGTA
TCTGAGGATG GAGAACTCAG CATAGAAAAC CCCTTTGGTG AAACATTTGG AAAAATACAA
GAAAGTGAAA AAACTCTTAT GATGAACACA TTATATAAGC TTCATGATCG ATTGGCACAG
CTTGCAGGAG ATCATGAATG TGGCAGTTCT AGTCAAAGAA CGCTTTCTGT TCAAGAGGCA
GCTGCGTATT TAAAAGATTT AGGTCCTGAA TATGAAGGTA TATTTAACAC TTCATTGCAG
TGGATCTTAG AAAATGGAAA AGATGTTGGA ATAAGGTGTG TTGGTTTTGG CCCTGAGGAA
GAATTGACAA ATATAACTGA TGTGCAGTTT TTACAGTCCA CAAGACCACT GATGTCTTTT
TGGTGTCGTT TTCGACGTGC TTTTGTTACT GTAACTCACA GATTATTGTT GTTATGCTTA
GGTGTAGTGA TGGTTTGTGT CGTTCTGCGT TACATGAAAT ATCGATGGAC AAAAGAAGAG
GAGGAAACAA GGCAGATGTA TGATATGGTG GTAAAGATTA TAGATGTTTT ACGAAGTCAT
AATGAAGCCT GCCAGGAAAA CAAAGATTTA CAACCTTACA TGCCTATTCC ACATGTACGC
GATTCCTTAA TACAGCCTCA TGACAGGAAA AAAATGAAGA AAGTCTGGGA TAGAGCTGTT
GACTTCCTTG CTGCTAATGA GTCTAGAGTT CGCACGGAAA CACGAAGAAT AGGTGGTGCA
GATTTTCTGG TTTGGCGGTG GATCCAGCCT TCTGCATCCT GTGACAAAAT ATTAGTTATA
CCTTCTAAAG TATGGCAAGG TCAAGCATTT CATTTAGATA GAAGAAATTC ACCACCAAAT
AGTTTGACAC CGTGTCTAAA GATTCGGAAT ATGTTTGATC CCGTTATGGA AATAGGGGAT
CAGTGGCATT TGGCAATTCA AGAAGCAATT TTAGAAAAAT GCAGTGATAA TGATGGCATT
GTTCACATTG CAGTAGACAA AAATTCACGT GAGGGTTGTG TATATGTTAA ATGTCTGTCT
CCAGAATATG CTGGAAAGGC TTTTAAAGCA TTGCATGGCT CTTGGTTTGA TGGGAAATTG
GTTACAGTAA AATATTTACG ACTAGATAGA TACCACCATC GCTTTCCCCA GGCTCTCACT
TCCAACACTC CATTGAAGCC ATCAAATAAA CATATGAACT CCATGTCTCA TCTTCGTCTT
CGGACTGGCC TAACCAATTC TCAAGGAAGT TCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu04065
Clone ID Related Accession (Same CDS sequence) NM_001167614
Accession Version NM_001167614.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2733bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product inner nuclear membrane protein Man1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK312473.1, AF112299.2 and BX390128.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK312473.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144335, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGCGGCGG CAGCAGCTTC GGCGCCTCAG CAGCTCTCGG ATGAGGAGCT TTTCTCTCAG 
CTCCGCCGTT ACGGCCTGTC TCCCGGACCA GTGACGGAGA GCACCCGCCC GGTCTACCTC
AAGAAGCTGA AGAAGCTTCG AGAGGAAGAG CAGCAACAGC ACCGGTCAGG GGGCCGCGGC
AACAAGACGC GGAACAGTAA TAACAATAAC ACGGCAGCCG CCACGGTCGC AGCCGCGGGA
CCAGCGGCGG CGGCGGCCGC GGGGATGGGG GTCCGGCCGG TCTCGGGCGA CCTCTCCTAC
TTACGGACTC CTGGGGGCCT GTGCCGAATC TCGGCCTCTG GCCCAGAGAG CCTCCTGGGA
GGGCCCGGGG GCGCCTCCGC CGCCCCCGCG GCTGGCAGCA AAGTGCTGCT GGGCTTCAGC
TCGGACGAGT CGGACGTGGA GGCCAGTCCC CGGGACCAGG CCGGCGGCGG CGGGAGGAAA
GACCGGGCTT CGCTCCAGTA CCGCGGGCTC AAAGCGCCGC CGGCGCCCCT GGCCGCCAGC
GAGGTGACTA ACAGCAACTC TGCAGAGCGA AGGAAGCCCC ACTCGTGGTG GGGGGCCAGG
AGGCCGGCGG GCCCCGAGCT GCAGACCCCG CCGGGGAAAG ATGGAGCAGT GGAGGACGAG
GAAGGGGAGG GAGAGGACGG TGAGGAGAGG GACCCGGAGA CCGAGGAGCC GCTCTGGGCG
AGCCGGACCG TGAATGGCAG CCGGCTTGTC CCCTACAGCT GCCGGGAAAA CTATTCGGAC
TCAGAGGAAG AGGACGACGA CGACGTGGCC TCCAGCAGAC AGGTATTAAA GGACGACTCC
CTTTCCCGGC ATCGGCCCAG ACGAACCCAT AGTAAGCCTC TCCCCCCGCT GACTGCTAAA
TCGGCCGGCG GCAGGCTGGA GACTTCAGTT CAGGGAGGGG GAGGACTCGC GATGAATGAC
AGGGCGGCGG CTGCCGGGAG TCTAGACAGG AGCCGAAACC TCGAAGAGGC GGCGGCCGCG
GAGCAGGGAG GAGGGTGTGA TCAAGTGGAC TCCAGCCCCG TTCCTAGATA CCGTGTTAAC
GCTAAGAAAC TGACCCCTCT CCTGCCCCCG CCACTTACTG ACATGGACTC AACCTTGGAT
TCGTCAACAG GCTCCCTTCT GAAAACCAAT AATCATATTG GCGGTGGGGC CTTCAGTGTG
GACTCCCCCA GGATTTATTC TAACAGTCTC CCTCCCAGTG CGGCGGTGGC CGCCTCTAGT
TCACTCAGGA TCAATCACGC CAATCATACG GGCTCCAATC ATACCTACCT GAAAAACACA
TACAACAAAC CGAAGCTTTC CGAACCCGAA GAGGAACTTC TCCAGCAATT TAAACGGGAG
GAGGTGTCCC CAACAGGGAG TTTCAGTGCC CACTACTTGT CGATGTTTCT CTTAACTGCT
GCCTGCTTAT TTTTCCTAAT ACTGGGACTG ACTTACCTAG GAATGAGAGG GACAGGAGTA
TCTGAGGATG GAGAACTCAG CAAAAACCCC TTTGGTGAAA CATTTGGAAA AATACAAGAA
AGTGAAAAAA CTCTTATGAT GAACACATTA TATAAGCTTC ATGATCGATT GGCACAGCTT
GCAGGAGATC ATGAATGTGG CAGTTCTAGT CAAAGAACGC TTTCTGTTCA AGAGGCAGCT
GCGTATTTAA AAGATTTAGG TCCTGAATAT GAAGGTATAT TTAACACTTC ATTGCAGTGG
ATCTTAGAAA ATGGAAAAGA TGTTGGAATA AGGTGTGTTG GTTTTGGCCC TGAGGAAGAA
TTGACAAATA TAACTGATGT GCAGTTTTTA CAGTCCACAA GACCACTGAT GTCTTTTTGG
TGTCGTTTTC GACGTGCTTT TGTTACTGTA ACTCACAGAT TATTGTTGTT ATGCTTAGGT
GTAGTGATGG TTTGTGTCGT TCTGCGTTAC ATGAAATATC GATGGACAAA AGAAGAGGAG
GAAACAAGGC AGATGTATGA TATGGTGGTA AAGATTATAG ATGTTTTACG AAGTCATAAT
GAAGCCTGCC AGGAAAACAA AGATTTACAA CCTTACATGC CTATTCCACA TGTACGCGAT
TCCTTAATAC AGCCTCATGA CAGGAAAAAA ATGAAGAAAG TCTGGGATAG AGCTGTTGAC
TTCCTTGCTG CTAATGAGTC TAGAGTTCGC ACGGAAACAC GAAGAATAGG TGGTGCAGAT
TTTCTGGTTT GGCGGTGGAT CCAGCCTTCT GCATCCTGTG ACAAAATATT AGTTATACCT
TCTAAAGTAT GGCAAGGTCA AGCATTTCAT TTAGATAGAA GAAATTCACC ACCAAATAGT
TTGACACCGT GTCTAAAGAT TCGGAATATG TTTGATCCCG TTATGGAAAT AGGGGATCAG
TGGCATTTGG CAATTCAAGA AGCAATTTTA GAAAAATGCA GTGATAATGA TGGCATTGTT
CACATTGCAG TAGACAAAAA TTCACGTGAG GGTTGTGTAT ATGTTAAATG TCTGTCTCCA
GAATATGCTG GAAAGGCTTT TAAAGCATTG CATGGCTCTT GGTTTGATGG GAAATTGGTT
ACAGTAAAAT ATTTACGACT AGATAGATAC CACCATCGCT TTCCCCAGGC TCTCACTTCC
AACACTCCAT TGAAGCCATC AAATAAACAT ATGAACTCCA TGTCTCATCT TCGTCTTCGG
ACTGGCCTAA CCAATTCTCA AGGAAGTTCC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001161086.1
CDS27..2759
Misc Feature(1)30..32(+)
Misc Feature(2)51..179(+)
Misc Feature(3)105..152(+)
Misc Feature(4)444..446(+)
Misc Feature(5)447..449(+)
Misc Feature(6)456..458(+)
Misc Feature(7)579..581(+)
Misc Feature(8)585..587(+)
Misc Feature(9)801..803(+)
Misc Feature(10)807..809(+)
Misc Feature(11)864..866(+)
Misc Feature(12)1080..1082(+)
Misc Feature(13)1119..1121(+)
Misc Feature(14)1230..1232(+)
Misc Feature(15)1449..1511(+)
Misc Feature(16)1578..2276(+)
Misc Feature(17)1905..1967(+)
Misc Feature(18)2118..2756(+)
Misc Feature(19)2352..2354(+)
Misc Feature(20)2376..2651(+)
Misc Feature(21)2670..2672(+)
Misc Feature(22)2754..2756(+)
Exon (1)1..1548
Gene:LEMD3
Gene Synonym:
Exon (2)1549..1583
Gene:LEMD3
Gene Synonym:
Exon (3)1584..1650
Gene:LEMD3
Gene Synonym:
Exon (4)1651..1718
Gene:LEMD3
Gene Synonym:
Exon (5)1719..1798
Gene:LEMD3
Gene Synonym:
Exon (6)1799..1944
Gene:LEMD3
Gene Synonym:
Exon (7)1945..2046
Gene:LEMD3
Gene Synonym:
Exon (8)2047..2149
Gene:LEMD3
Gene Synonym:
Exon (9)2150..2328
Gene:LEMD3
Gene Synonym:
Exon (10)2329..2410
Gene:LEMD3
Gene Synonym:
Exon (11)2411..2516
Gene:LEMD3
Gene Synonym:
Exon (12)2517..2595
Gene:LEMD3
Gene Synonym:
Exon (13)2596..4795
Gene:LEMD3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001167614
Organism Homo sapiens (human)
Definition Homo sapiens LEM domain containing 3 (LEMD3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001167614

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGCGGCGG CAGCAGCTTC GGCGCCTCAG CAGCTCTCGG ATGAGGAGCT TTTCTCTCAG 
CTCCGCCGTT ACGGCCTGTC TCCCGGACCA GTGACGGAGA GCACCCGCCC GGTCTACCTC
AAGAAGCTGA AGAAGCTTCG AGAGGAAGAG CAGCAACAGC ACCGGTCAGG GGGCCGCGGC
AACAAGACGC GGAACAGTAA TAACAATAAC ACGGCAGCCG CCACGGTCGC AGCCGCGGGA
CCAGCGGCGG CGGCGGCCGC GGGGATGGGG GTCCGGCCGG TCTCGGGCGA CCTCTCCTAC
TTACGGACTC CTGGGGGCCT GTGCCGAATC TCGGCCTCTG GCCCAGAGAG CCTCCTGGGA
GGGCCCGGGG GCGCCTCCGC CGCCCCCGCG GCTGGCAGCA AAGTGCTGCT GGGCTTCAGC
TCGGACGAGT CGGACGTGGA GGCCAGTCCC CGGGACCAGG CCGGCGGCGG CGGGAGGAAA
GACCGGGCTT CGCTCCAGTA CCGCGGGCTC AAAGCGCCGC CGGCGCCCCT GGCCGCCAGC
GAGGTGACTA ACAGCAACTC TGCAGAGCGA AGGAAGCCCC ACTCGTGGTG GGGGGCCAGG
AGGCCGGCGG GCCCCGAGCT GCAGACCCCG CCGGGGAAAG ATGGAGCAGT GGAGGACGAG
GAAGGGGAGG GAGAGGACGG TGAGGAGAGG GACCCGGAGA CCGAGGAGCC GCTCTGGGCG
AGCCGGACCG TGAATGGCAG CCGGCTTGTC CCCTACAGCT GCCGGGAAAA CTATTCGGAC
TCAGAGGAAG AGGACGACGA CGACGTGGCC TCCAGCAGAC AGGTATTAAA GGACGACTCC
CTTTCCCGGC ATCGGCCCAG ACGAACCCAT AGTAAGCCTC TCCCCCCGCT GACTGCTAAA
TCGGCCGGCG GCAGGCTGGA GACTTCAGTT CAGGGAGGGG GAGGACTCGC GATGAATGAC
AGGGCGGCGG CTGCCGGGAG TCTAGACAGG AGCCGAAACC TCGAAGAGGC GGCGGCCGCG
GAGCAGGGAG GAGGGTGTGA TCAAGTGGAC TCCAGCCCCG TTCCTAGATA CCGTGTTAAC
GCTAAGAAAC TGACCCCTCT CCTGCCCCCG CCACTTACTG ACATGGACTC AACCTTGGAT
TCGTCAACAG GCTCCCTTCT GAAAACCAAT AATCATATTG GCGGTGGGGC CTTCAGTGTG
GACTCCCCCA GGATTTATTC TAACAGTCTC CCTCCCAGTG CGGCGGTGGC CGCCTCTAGT
TCACTCAGGA TCAATCACGC CAATCATACG GGCTCCAATC ATACCTACCT GAAAAACACA
TACAACAAAC CGAAGCTTTC CGAACCCGAA GAGGAACTTC TCCAGCAATT TAAACGGGAG
GAGGTGTCCC CAACAGGGAG TTTCAGTGCC CACTACTTGT CGATGTTTCT CTTAACTGCT
GCCTGCTTAT TTTTCCTAAT ACTGGGACTG ACTTACCTAG GAATGAGAGG GACAGGAGTA
TCTGAGGATG GAGAACTCAG CAAAAACCCC TTTGGTGAAA CATTTGGAAA AATACAAGAA
AGTGAAAAAA CTCTTATGAT GAACACATTA TATAAGCTTC ATGATCGATT GGCACAGCTT
GCAGGAGATC ATGAATGTGG CAGTTCTAGT CAAAGAACGC TTTCTGTTCA AGAGGCAGCT
GCGTATTTAA AAGATTTAGG TCCTGAATAT GAAGGTATAT TTAACACTTC ATTGCAGTGG
ATCTTAGAAA ATGGAAAAGA TGTTGGAATA AGGTGTGTTG GTTTTGGCCC TGAGGAAGAA
TTGACAAATA TAACTGATGT GCAGTTTTTA CAGTCCACAA GACCACTGAT GTCTTTTTGG
TGTCGTTTTC GACGTGCTTT TGTTACTGTA ACTCACAGAT TATTGTTGTT ATGCTTAGGT
GTAGTGATGG TTTGTGTCGT TCTGCGTTAC ATGAAATATC GATGGACAAA AGAAGAGGAG
GAAACAAGGC AGATGTATGA TATGGTGGTA AAGATTATAG ATGTTTTACG AAGTCATAAT
GAAGCCTGCC AGGAAAACAA AGATTTACAA CCTTACATGC CTATTCCACA TGTACGCGAT
TCCTTAATAC AGCCTCATGA CAGGAAAAAA ATGAAGAAAG TCTGGGATAG AGCTGTTGAC
TTCCTTGCTG CTAATGAGTC TAGAGTTCGC ACGGAAACAC GAAGAATAGG TGGTGCAGAT
TTTCTGGTTT GGCGGTGGAT CCAGCCTTCT GCATCCTGTG ACAAAATATT AGTTATACCT
TCTAAAGTAT GGCAAGGTCA AGCATTTCAT TTAGATAGAA GAAATTCACC ACCAAATAGT
TTGACACCGT GTCTAAAGAT TCGGAATATG TTTGATCCCG TTATGGAAAT AGGGGATCAG
TGGCATTTGG CAATTCAAGA AGCAATTTTA GAAAAATGCA GTGATAATGA TGGCATTGTT
CACATTGCAG TAGACAAAAA TTCACGTGAG GGTTGTGTAT ATGTTAAATG TCTGTCTCCA
GAATATGCTG GAAAGGCTTT TAAAGCATTG CATGGCTCTT GGTTTGATGG GAAATTGGTT
ACAGTAAAAT ATTTACGACT AGATAGATAC CACCATCGCT TTCCCCAGGC TCTCACTTCC
AACACTCCAT TGAAGCCATC AAATAAACAT ATGAACTCCA TGTCTCATCT TCGTCTTCGG
ACTGGCCTAA CCAATTCTCA AGGAAGTTCC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Inhibition of TGF-beta signaling at the nuclear envelope: characterization of interactions between MAN1, Smad2 and Smad3, and PPM1A
Sci Signal 6 (280), RA49 (2013)
Bourgeois B, Gilquin B, Tellier-Lebegue C, Ostlund C, Wu W, Perez J, El Hage P, Lallemand F, Worman HJ and Zinn-Justin S.


book

Buschke-Ollendorff syndrome with striking phenotypic variation resulting from a novel c.2203C>T nonsense mutation in LEMD3
Pediatr Dermatol 28 (4), 447-450 (2011)
Yuste-Chaves M, Canueto J, Santos-Briz A, Ciria S, Gonzalez-Sarmiento R and Unamuno P.


book

No interaction of barrier-to-autointegration factor (BAF) with HIV-1 MA, cone-rod homeobox (Crx) or MAN1-C in absence of DNA
PLoS ONE 6 (9), E25123 (2011)
Huang Y, Cai M, Clore GM and Craigie R.


book

Buschke-Ollendorff syndrome in a three-generation family: influence of a novel LEMD3 mutation to tropoelastin expression
Eur J Dermatol 20 (6), 693-697 (2010)
Burger B, Hershkovitz D, Indelman M, Kovac M, Galambos J, Haeusermann P, Sprecher E and Itin PH.


book

The integral inner nuclear membrane protein MAN1 physically interacts with the R-Smad proteins to repress signaling by the transforming growth factor-{beta} superfamily of cytokines
J. Biol. Chem. 280 (16), 15992-16001 (2005)
Pan D, Estevez-Salmeron LD, Stroschein SL, Zhu X, He J, Zhou S and Luo K.


book

Direct binding of nuclear membrane protein MAN1 to emerin in vitro and two modes of binding to barrier-to-autointegration factor
J. Biol. Chem. 280 (14), 13863-13870 (2005)
Mansharamani M and Wilson KL.


book

MAN1, an integral protein of the inner nuclear membrane, binds Smad2 and Smad3 and antagonizes transforming growth factor-beta signaling
Hum. Mol. Genet. 14 (3), 437-445 (2005)
Lin F, Morrison JM, Wu W and Worman HJ.


book

Loss-of-function mutations in LEMD3 result in osteopoikilosis, Buschke-Ollendorff syndrome and melorheostosis
Nat. Genet. 36 (11), 1213-1218 (2004)
Hellemans J, Preobrazhenska O, Willaert A, Debeer P, Verdonk PC, Costa T, Janssens K, Menten B, Van Roy N, Vermeulen SJ, Savarirayan R, Van Hul W, Vanhoenacker F, Huylebroeck D, De Paepe A, Naeyaert JM, Vandesompele J, Speleman F, Verschueren K, Coucke PJ and Mortier GR.


book

Intracellular trafficking of MAN1, an integral protein of the nuclear envelope inner membrane
J. Cell. Sci. 115 (PT 7), 1361-1371 (2002)
Wu W, Lin F and Worman HJ.


book

MAN1, an inner nuclear membrane protein that shares the LEM domain with lamina-associated polypeptide 2 and emerin
J. Biol. Chem. 275 (7), 4840-4847 (2000)
Lin F, Blake DL, Callebaut I, Skerjanc IS, Holmer L, McBurney MW, Paulin-Levasseur M and Worman HJ.


 
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