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ALPL cDNA ORF clone, Homo sapiens(Human)

Gene Symbol ALPL
Entrez Gene ID 249
Full Name alkaline phosphatase, liver/bone/kidney
Synonyms AP-TNAP,APTNAP,HOPS,TNAP,TNSALP
General protein information
Preferred Names

alkaline phosphatase, liver/bone/kidney

Names

alkaline phosphatase, tissue-nonspecific isozyme
alkaline phosphatase liver/bone/kidney isozyme
liver/bone/kidney-type alkaline phosphatase
tissue-nonspecific ALP

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

1

1p36.12

Summary This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. [provided by RefSeq, Oct 2015].
Disorder MIM:

171760

Disorder Html: Hypophosphatasia, infantile, 241500 (3); Hypophosphatasia, childhood,

mRNA and Protein(s)

mRNA Protein Name
NM_001127501.3 NP_001120973.2 alkaline phosphatase, tissue-nonspecific isozyme isoform 2
NM_001177520.2 NP_001170991.1 alkaline phosphatase, tissue-nonspecific isozyme isoform 3
XM_006710546.2 XP_006710609.1 alkaline phosphatase, tissue-nonspecific isozyme isoform X1
XM_017000903.1 XP_016856392.1 alkaline phosphatase, tissue-nonspecific isozyme isoform X2
NM_000478.5 NP_000469.3 alkaline phosphatase, tissue-nonspecific isozyme isoform 1 preproprotein
XM_005245818.1 XP_005245875.1 alkaline phosphatase, tissue-nonspecific isozyme isoform X1



Bos taurus (cattle) ALPL NP_789828.2
Canis lupus familiaris (dog) ALPL NP_001184066.1
Rattus norvegicus (Norway rat) Alpl NP_037191.1
Gallus gallus (chicken) ALPL NP_990691.1
Drosophila melanogaster (fruit fly) CG8147 NP_649897.4
Macaca mulatta (rhesus monkey) ALPL XP_001109717.2
Homo sapiens (human) ALPL NP_000469.3
Anopheles gambiae (African malaria mosquito) AgaP_AGAP001684 XP_321411.3
Pan troglodytes (chimpanzee) ALPL XP_003307915.1
Xenopus tropicalis (tropical clawed frog) LOC100485467 XP_002943485.2
Mus musculus (house mouse) Alpl NP_001274101.1
Danio rerio (zebrafish) alp3 NP_001265768.1


Related articles in PubMed

Human gingiva transcriptome during wound healing.
Wang Y, Tatakis DN
Journal of clinical periodontology44(4)394-402(2017 Apr)

Molecular defect of tissue-nonspecific alkaline phosphatase bearing a substitution at position 426 associated with hypophosphatasia.
Al-Shawafi HA, Komaru K, Oda K
Molecular and cellular biochemistry427(1-2)169-176(2017 Mar)

Serum Alkaline Phosphatase Levels are Not Associated with Increased Death Risk in Prevalent Hemodialysis Patients: 5-Year Experience in a Single Hemodialysis Center.
Zhu JG, Cheng BC, Lee WC, Li LC, Lee CH, Long G, Chen JB
Kidney & blood pressure research41(4)498-506(2016)

Immunofluorescence Labeling of a Mutant of Tissue Non-Specific Alkaline Phosphatase Lacking the Glysosylphosphatidylinositol Anchor.
Matsumura T, Saito S, Orimo H
Journal of Nippon Medical School = Nippon Ika Daigaku zasshi83(4)140-1(2016)

The role of phosphatases in the initiation of skeletal mineralization.
Millán JL
Calcified tissue international93(4)299-306(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

His ALPL gene mutation came from c.228delG mutation in his mother and c.407G>A compound heterozygous mutation in his father
Title: [Infantile hypophosphatasia caused by a novel compound heterozygous mutation: a case report and pedigree analysis].

Both PPARgamma gene expression and TNALP activity increased during intracellular lipid accumulation in HepG2 and 3T3-L1 cells. Inhibition of TNALP blocked intracellular lipid accumulation but did not alter expression of the PPARgamma gene.
Title: The role of alkaline phosphatase in intracellular lipid accumulation in the human hepatocarcinoma cell line, HepG2.

ALPL expression is significantly upregulated in human masticatory mucosa during wound healing
Title: Human gingiva transcriptome during wound healing.

results reveal that the amino acid substitutions at position 426 of TNSALP differentially affect the structure and function of TNSALP, leading to understanding of the molecular and cellular basis of hypophosphatasia.
Title: Molecular defect of tissue-nonspecific alkaline phosphatase bearing a substitution at position 426 associated with hypophosphatasia.

ALPL is a major contributor to the pathogenesis of Prostate cancer progression.
Title: Tumour-derived alkaline phosphatase regulates tumour growth, epithelial plasticity and disease-free survival in metastatic prostate cancer.

The following ALPL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALPL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu22555 NM_001127501.3
Latest version!
Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $265.30
$379.00
OHu23066 NM_000478.5
Latest version!
Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $265.30
$379.00
OHu23043 NM_001177520.2
Latest version!
Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu33310 XM_006710546.2
Latest version!
Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu33310 XM_005245818.1
Latest version!
Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu87169 XM_017000903.1
Latest version!
Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22555
Clone ID Related Accession (Same CDS sequence) NM_001127501.3
Accession Version NM_001127501.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1410bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product alkaline phosphatase, tissue-nonspecific isozyme isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY052024.1, AK298085.1, BC021289.2 and AL359815.26. On Jan 8, 2016 this sequence version replaced NM_001127501.2. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) lacks a predicted signal peptide and is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298085.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

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361
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481
541
601
661
721
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841
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961
1021
1081
1141
1201
1261
1321
1381
ATGTTCCTGG GAGATGGGAT GGGTGTCTCC ACAGTGACGG CTGCCCGCAT CCTCAAGGGT 
CAGCTCCACC ACAACCCTGG GGAGGAGACC AGGCTGGAGA TGGACAAGTT CCCCTTCGTG
GCCCTCTCCA AGACGTACAA CACCAATGCC CAGGTCCCTG ACAGCGCCGG CACCGCCACC
GCCTACCTGT GTGGGGTGAA GGCCAATGAG GGCACCGTGG GGGTAAGCGC AGCCACTGAG
CGTTCCCGGT GCAACACCAC CCAGGGGAAC GAGGTCACCT CCATCCTGCG CTGGGCCAAG
GACGCTGGGA AATCTGTGGG CATTGTGACC ACCACGAGAG TGAACCATGC CACCCCCAGC
GCCGCCTACG CCCACTCGGC TGACCGGGAC TGGTACTCAG ACAACGAGAT GCCCCCTGAG
GCCTTGAGCC AGGGCTGTAA GGACATCGCC TACCAGCTCA TGCATAACAT CAGGGACATT
GACGTGATCA TGGGGGGTGG CCGGAAATAC ATGTACCCCA AGAATAAAAC TGATGTGGAG
TATGAGAGTG ACGAGAAAGC CAGGGGCACG AGGCTGGACG GCCTGGACCT CGTTGACACC
TGGAAGAGCT TCAAACCGAG ATACAAGCAC TCCCACTTCA TCTGGAACCG CACGGAACTC
CTGACCCTTG ACCCCCACAA TGTGGACTAC CTATTGGGTC TCTTCGAGCC AGGGGACATG
CAGTACGAGC TGAACAGGAA CAACGTGACG GACCCGTCAC TCTCCGAGAT GGTGGTGGTG
GCCATCCAGA TCCTGCGGAA GAACCCCAAA GGCTTCTTCT TGCTGGTGGA AGGAGGCAGA
ATTGACCACG GGCACCATGA AGGAAAAGCC AAGCAGGCCC TGCATGAGGC GGTGGAGATG
GACCGGGCCA TCGGGCAGGC AGGCAGCTTG ACCTCCTCGG AAGACACTCT GACCGTGGTC
ACTGCGGACC ATTCCCACGT CTTCACATTT GGTGGATACA CCCCCCGTGG CAACTCTATC
TTTGGTCTGG CCCCCATGCT GAGTGACACA GACAAGAAGC CCTTCACTGC CATCCTGTAT
GGCAATGGGC CTGGCTACAA GGTGGTGGGC GGTGAACGAG AGAATGTCTC CATGGTGGAC
TATGCTCACA ACAACTACCA GGCGCAGTCT GCTGTGCCCC TGCGCCACGA GACCCACGGC
GGGGAGGACG TGGCCGTCTT CTCCAAGGGC CCCATGGCGC ACCTGCTGCA CGGCGTCCAC
GAGCAGAACT ACGTCCCCCA CGTGATGGCG TATGCAGCCT GCATCGGGGC CAACCTCGGC
CACTGTGCTC CTGCCAGCTC GGCAGGCAGC CTTGCTGCAG GCCCCCTGCT GCTCGCGCTG
GCCCTCTACC CCCTGAGCGT CCTGTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001120973.2
CDS265..1674
Misc Feature(1)265..1569
Misc Feature(2)order(277..279,424..429,616..618,649..651,1093..1095,1108..1110,1120..1122,1231..1236,1459..1461)
Misc Feature(3)order(277..279,424..429,649..651,1108..1110,1120..1122,1234..1236,1459..1461)
Misc Feature(4)order(277..279,427..429,616..618,1093..1095,1108..1110,1120..1122,1231..1236,1459..1461)
Misc Feature(5)order(289..294,301..303,310..315,382..390,394..396,400..402,406..408,418..420,457..465,493..495,538..540,1456..1458,1462..1473,1477..1479,1507..1509,1513..1527,1531..1536,1543..1545)
Exon (1)1..160
Exon (2)161..280
Exon (3)281..396
Exon (4)397..571
Exon (5)572..747
Exon (6)748..891
Exon (7)892..961
Exon (8)962..1096
Exon (9)1097..1288
Exon (10)1289..1408
Exon (11)1409..2438
Translation

Target ORF information:

RefSeq Version NM_001127501.3
Organism Homo sapiens(Human)
Definition Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001127501.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGTTCCTGG GAGATGGGAT GGGTGTCTCC ACAGTGACGG CTGCCCGCAT CCTCAAGGGT 
CAGCTCCACC ACAACCCTGG GGAGGAGACC AGGCTGGAGA TGGACAAGTT CCCCTTCGTG
GCCCTCTCCA AGACGTACAA CACCAATGCC CAGGTCCCTG ACAGCGCCGG CACCGCCACC
GCCTACCTGT GTGGGGTGAA GGCCAATGAG GGCACCGTGG GGGTAAGCGC AGCCACTGAG
CGTTCCCGGT GCAACACCAC CCAGGGGAAC GAGGTCACCT CCATCCTGCG CTGGGCCAAG
GACGCTGGGA AATCTGTGGG CATTGTGACC ACCACGAGAG TGAACCATGC CACCCCCAGC
GCCGCCTACG CCCACTCGGC TGACCGGGAC TGGTACTCAG ACAACGAGAT GCCCCCTGAG
GCCTTGAGCC AGGGCTGTAA GGACATCGCC TACCAGCTCA TGCATAACAT CAGGGACATT
GACGTGATCA TGGGGGGTGG CCGGAAATAC ATGTACCCCA AGAATAAAAC TGATGTGGAG
TATGAGAGTG ACGAGAAAGC CAGGGGCACG AGGCTGGACG GCCTGGACCT CGTTGACACC
TGGAAGAGCT TCAAACCGAG ATACAAGCAC TCCCACTTCA TCTGGAACCG CACGGAACTC
CTGACCCTTG ACCCCCACAA TGTGGACTAC CTATTGGGTC TCTTCGAGCC AGGGGACATG
CAGTACGAGC TGAACAGGAA CAACGTGACG GACCCGTCAC TCTCCGAGAT GGTGGTGGTG
GCCATCCAGA TCCTGCGGAA GAACCCCAAA GGCTTCTTCT TGCTGGTGGA AGGAGGCAGA
ATTGACCACG GGCACCATGA AGGAAAAGCC AAGCAGGCCC TGCATGAGGC GGTGGAGATG
GACCGGGCCA TCGGGCAGGC AGGCAGCTTG ACCTCCTCGG AAGACACTCT GACCGTGGTC
ACTGCGGACC ATTCCCACGT CTTCACATTT GGTGGATACA CCCCCCGTGG CAACTCTATC
TTTGGTCTGG CCCCCATGCT GAGTGACACA GACAAGAAGC CCTTCACTGC CATCCTGTAT
GGCAATGGGC CTGGCTACAA GGTGGTGGGC GGTGAACGAG AGAATGTCTC CATGGTGGAC
TATGCTCACA ACAACTACCA GGCGCAGTCT GCTGTGCCCC TGCGCCACGA GACCCACGGC
GGGGAGGACG TGGCCGTCTT CTCCAAGGGC CCCATGGCGC ACCTGCTGCA CGGCGTCCAC
GAGCAGAACT ACGTCCCCCA CGTGATGGCG TATGCAGCCT GCATCGGGGC CAACCTCGGC
CACTGTGCTC CTGCCAGCTC GGCAGGCAGC CTTGCTGCAG GCCCCCTGCT GCTCGCGCTG
GCCCTCTACC CCCTGAGCGT CCTGTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23066
Clone ID Related Accession (Same CDS sequence) NM_000478.5
Accession Version NM_000478.5 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1575bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product alkaline phosphatase, tissue-nonspecific isozyme isoform 1 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY052024.1, BC021289.2 and AL359815.26. This sequence is a reference standard in the RefSeqGene project. On Jan 8, 2016 this sequence version replaced NM_000478.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC021289.2, AB209814.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
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361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGATTTCAC CATTCTTAGT ACTGGCCATT GGCACCTGCC TTACTAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTACTGGCGA GACCAAGCGC AAGAGACACT GAAATATGCC
CTGGAGCTTC AGAAGCTCAA CACCAACGTG GCTAAGAATG TCATCATGTT CCTGGGAGAT
GGGATGGGTG TCTCCACAGT GACGGCTGCC CGCATCCTCA AGGGTCAGCT CCACCACAAC
CCTGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCCT TCGTGGCCCT CTCCAAGACG
TACAACACCA ATGCCCAGGT CCCTGACAGC GCCGGCACCG CCACCGCCTA CCTGTGTGGG
GTGAAGGCCA ATGAGGGCAC CGTGGGGGTA AGCGCAGCCA CTGAGCGTTC CCGGTGCAAC
ACCACCCAGG GGAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT
GTGGGCATTG TGACCACCAC GAGAGTGAAC CATGCCACCC CCAGCGCCGC CTACGCCCAC
TCGGCTGACC GGGACTGGTA CTCAGACAAC GAGATGCCCC CTGAGGCCTT GAGCCAGGGC
TGTAAGGACA TCGCCTACCA GCTCATGCAT AACATCAGGG ACATTGACGT GATCATGGGG
GGTGGCCGGA AATACATGTA CCCCAAGAAT AAAACTGATG TGGAGTATGA GAGTGACGAG
AAAGCCAGGG GCACGAGGCT GGACGGCCTG GACCTCGTTG ACACCTGGAA GAGCTTCAAA
CCGAGATACA AGCACTCCCA CTTCATCTGG AACCGCACGG AACTCCTGAC CCTTGACCCC
CACAATGTGG ACTACCTATT GGGTCTCTTC GAGCCAGGGG ACATGCAGTA CGAGCTGAAC
AGGAACAACG TGACGGACCC GTCACTCTCC GAGATGGTGG TGGTGGCCAT CCAGATCCTG
CGGAAGAACC CCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGAATTGA CCACGGGCAC
CATGAAGGAA AAGCCAAGCA GGCCCTGCAT GAGGCGGTGG AGATGGACCG GGCCATCGGG
CAGGCAGGCA GCTTGACCTC CTCGGAAGAC ACTCTGACCG TGGTCACTGC GGACCATTCC
CACGTCTTCA CATTTGGTGG ATACACCCCC CGTGGCAACT CTATCTTTGG TCTGGCCCCC
ATGCTGAGTG ACACAGACAA GAAGCCCTTC ACTGCCATCC TGTATGGCAA TGGGCCTGGC
TACAAGGTGG TGGGCGGTGA ACGAGAGAAT GTCTCCATGG TGGACTATGC TCACAACAAC
TACCAGGCGC AGTCTGCTGT GCCCCTGCGC CACGAGACCC ACGGCGGGGA GGACGTGGCC
GTCTTCTCCA AGGGCCCCAT GGCGCACCTG CTGCACGGCG TCCACGAGCA GAACTACGTC
CCCCACGTGA TGGCGTATGC AGCCTGCATC GGGGCCAACC TCGGCCACTG TGCTCCTGCC
AGCTCGGCAG GCAGCCTTGC TGCAGGCCCC CTGCTGCTCG CGCTGGCCCT CTACCCCCTG
AGCGTCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000469.3
CDS265..1839
Misc Feature(1)415..1734
Misc Feature(2)order(442..444,589..594,781..783,814..816,1258..1260,1273..1275,1285..1287,1396..1401,1624..1626)
Misc Feature(3)order(442..444,589..594,814..816,1273..1275,1285..1287,1399..1401,1624..1626)
Misc Feature(4)order(442..444,592..594,781..783,1258..1260,1273..1275,1285..1287,1396..1401,1624..1626)
Misc Feature(5)order(454..459,466..468,475..480,547..555,559..561,565..567,571..573,583..585,622..630,658..660,703..705,1621..1623,1627..1638,1642..1644,1672..1674,1678..1692,1696..1701,1708..1710)
Misc Feature(6)592..594
Exon (1)1..160
Exon (2)161..325
Exon (3)326..445
Exon (4)446..561
Exon (5)562..736
Exon (6)737..912
Exon (7)913..1056
Exon (8)1057..1126
Exon (9)1127..1261
Exon (10)1262..1453
Exon (11)1454..1573
Exon (12)1574..2603
Translation

Target ORF information:

RefSeq Version NM_000478.5
Organism Homo sapiens(Human)
Definition Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000478.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGATTTCAC CATTCTTAGT ACTGGCCATT GGCACCTGCC TTACTAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTACTGGCGA GACCAAGCGC AAGAGACACT GAAATATGCC
CTGGAGCTTC AGAAGCTCAA CACCAACGTG GCTAAGAATG TCATCATGTT CCTGGGAGAT
GGGATGGGTG TCTCCACAGT GACGGCTGCC CGCATCCTCA AGGGTCAGCT CCACCACAAC
CCTGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCCT TCGTGGCCCT CTCCAAGACG
TACAACACCA ATGCCCAGGT CCCTGACAGC GCCGGCACCG CCACCGCCTA CCTGTGTGGG
GTGAAGGCCA ATGAGGGCAC CGTGGGGGTA AGCGCAGCCA CTGAGCGTTC CCGGTGCAAC
ACCACCCAGG GGAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT
GTGGGCATTG TGACCACCAC GAGAGTGAAC CATGCCACCC CCAGCGCCGC CTACGCCCAC
TCGGCTGACC GGGACTGGTA CTCAGACAAC GAGATGCCCC CTGAGGCCTT GAGCCAGGGC
TGTAAGGACA TCGCCTACCA GCTCATGCAT AACATCAGGG ACATTGACGT GATCATGGGG
GGTGGCCGGA AATACATGTA CCCCAAGAAT AAAACTGATG TGGAGTATGA GAGTGACGAG
AAAGCCAGGG GCACGAGGCT GGACGGCCTG GACCTCGTTG ACACCTGGAA GAGCTTCAAA
CCGAGATACA AGCACTCCCA CTTCATCTGG AACCGCACGG AACTCCTGAC CCTTGACCCC
CACAATGTGG ACTACCTATT GGGTCTCTTC GAGCCAGGGG ACATGCAGTA CGAGCTGAAC
AGGAACAACG TGACGGACCC GTCACTCTCC GAGATGGTGG TGGTGGCCAT CCAGATCCTG
CGGAAGAACC CCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGAATTGA CCACGGGCAC
CATGAAGGAA AAGCCAAGCA GGCCCTGCAT GAGGCGGTGG AGATGGACCG GGCCATCGGG
CAGGCAGGCA GCTTGACCTC CTCGGAAGAC ACTCTGACCG TGGTCACTGC GGACCATTCC
CACGTCTTCA CATTTGGTGG ATACACCCCC CGTGGCAACT CTATCTTTGG TCTGGCCCCC
ATGCTGAGTG ACACAGACAA GAAGCCCTTC ACTGCCATCC TGTATGGCAA TGGGCCTGGC
TACAAGGTGG TGGGCGGTGA ACGAGAGAAT GTCTCCATGG TGGACTATGC TCACAACAAC
TACCAGGCGC AGTCTGCTGT GCCCCTGCGC CACGAGACCC ACGGCGGGGA GGACGTGGCC
GTCTTCTCCA AGGGCCCCAT GGCGCACCTG CTGCACGGCG TCCACGAGCA GAACTACGTC
CCCCACGTGA TGGCGTATGC AGCCTGCATC GGGGCCAACC TCGGCCACTG TGCTCCTGCC
AGCTCGGCAG GCAGCCTTGC TGCAGGCCCC CTGCTGCTCG CGCTGGCCCT CTACCCCCTG
AGCGTCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23043
Clone ID Related Accession (Same CDS sequence) NM_001177520.2
Accession Version NM_001177520.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1344bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product alkaline phosphatase, tissue-nonspecific isozyme isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY052024.1, AK295608.1, BC021289.2 and AL359815.26. On Jan 8, 2016 this sequence version replaced NM_001177520.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. This variant also lacks an exon in the 5' coding region, compared to variant 1. The encoded isoform (3) has a distinct N-terminus, lacks a predicted signal peptide, and is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK295608.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146236, SAMEA2159764 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCCCTGGA GCTTCAGAAG CTCAACACCA ACGTGGCTAA GAATGTCATC ATGTTCCTGG 
GAGATGACGT ACAACACCAA TGCCCAGGTC CCTGACAGCG CCGGCACCGC CACCGCCTAC
CTGTGTGGGG TGAAGGCCAA TGAGGGCACC GTGGGGGTAA GCGCAGCCAC TGAGCGTTCC
CGGTGCAACA CCACCCAGGG GAACGAGGTC ACCTCCATCC TGCGCTGGGC CAAGGACGCT
GGGAAATCTG TGGGCATTGT GACCACCACG AGAGTGAACC ATGCCACCCC CAGCGCCGCC
TACGCCCACT CGGCTGACCG GGACTGGTAC TCAGACAACG AGATGCCCCC TGAGGCCTTG
AGCCAGGGCT GTAAGGACAT CGCCTACCAG CTCATGCATA ACATCAGGGA CATTGACGTG
ATCATGGGGG GTGGCCGGAA ATACATGTAC CCCAAGAATA AAACTGATGT GGAGTATGAG
AGTGACGAGA AAGCCAGGGG CACGAGGCTG GACGGCCTGG ACCTCGTTGA CACCTGGAAG
AGCTTCAAAC CGAGATACAA GCACTCCCAC TTCATCTGGA ACCGCACGGA ACTCCTGACC
CTTGACCCCC ACAATGTGGA CTACCTATTG GGTCTCTTCG AGCCAGGGGA CATGCAGTAC
GAGCTGAACA GGAACAACGT GACGGACCCG TCACTCTCCG AGATGGTGGT GGTGGCCATC
CAGATCCTGC GGAAGAACCC CAAAGGCTTC TTCTTGCTGG TGGAAGGAGG CAGAATTGAC
CACGGGCACC ATGAAGGAAA AGCCAAGCAG GCCCTGCATG AGGCGGTGGA GATGGACCGG
GCCATCGGGC AGGCAGGCAG CTTGACCTCC TCGGAAGACA CTCTGACCGT GGTCACTGCG
GACCATTCCC ACGTCTTCAC ATTTGGTGGA TACACCCCCC GTGGCAACTC TATCTTTGGT
CTGGCCCCCA TGCTGAGTGA CACAGACAAG AAGCCCTTCA CTGCCATCCT GTATGGCAAT
GGGCCTGGCT ACAAGGTGGT GGGCGGTGAA CGAGAGAATG TCTCCATGGT GGACTATGCT
CACAACAACT ACCAGGCGCA GTCTGCTGTG CCCCTGCGCC ACGAGACCCA CGGCGGGGAG
GACGTGGCCG TCTTCTCCAA GGGCCCCATG GCGCACCTGC TGCACGGCGT CCACGAGCAG
AACTACGTCC CCCACGTGAT GGCGTATGCA GCCTGCATCG GGGCCAACCT CGGCCACTGT
GCTCCTGCCA GCTCGGCAGG CAGCCTTGCT GCAGGCCCCC TGCTGCTCGC GCTGGCCCTC
TACCCCCTGA GCGTCCTGTT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001170991.1
CDS215..1558
Misc Feature(1)209..211
Misc Feature(2)order(266..274,278..280,284..286,290..292,302..304,341..349,377..379,422..424,1340..1342,1346..1357,1361..1363,1391..1393,1397..1411,1415..1420,1427..1429)
Misc Feature(3)278..1453
Misc Feature(4)order(308..313,500..502,533..535,977..979,992..994,1004..1006,1115..1120,1343..1345)
Misc Feature(5)order(308..313,533..535,992..994,1004..1006,1118..1120,1343..1345)
Exon (1)1..160
Exon (2)161..280
Exon (3)281..455
Exon (4)456..631
Exon (5)632..775
Exon (6)776..845
Exon (7)846..980
Exon (8)981..1172
Exon (9)1173..1292
Exon (10)1293..2322
Translation

Target ORF information:

RefSeq Version NM_001177520.2
Organism Homo sapiens(Human)
Definition Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001177520.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCCCTGGA GCTTCAGAAG CTCAACACCA ACGTGGCTAA GAATGTCATC ATGTTCCTGG 
GAGATGACGT ACAACACCAA TGCCCAGGTC CCTGACAGCG CCGGCACCGC CACCGCCTAC
CTGTGTGGGG TGAAGGCCAA TGAGGGCACC GTGGGGGTAA GCGCAGCCAC TGAGCGTTCC
CGGTGCAACA CCACCCAGGG GAACGAGGTC ACCTCCATCC TGCGCTGGGC CAAGGACGCT
GGGAAATCTG TGGGCATTGT GACCACCACG AGAGTGAACC ATGCCACCCC CAGCGCCGCC
TACGCCCACT CGGCTGACCG GGACTGGTAC TCAGACAACG AGATGCCCCC TGAGGCCTTG
AGCCAGGGCT GTAAGGACAT CGCCTACCAG CTCATGCATA ACATCAGGGA CATTGACGTG
ATCATGGGGG GTGGCCGGAA ATACATGTAC CCCAAGAATA AAACTGATGT GGAGTATGAG
AGTGACGAGA AAGCCAGGGG CACGAGGCTG GACGGCCTGG ACCTCGTTGA CACCTGGAAG
AGCTTCAAAC CGAGATACAA GCACTCCCAC TTCATCTGGA ACCGCACGGA ACTCCTGACC
CTTGACCCCC ACAATGTGGA CTACCTATTG GGTCTCTTCG AGCCAGGGGA CATGCAGTAC
GAGCTGAACA GGAACAACGT GACGGACCCG TCACTCTCCG AGATGGTGGT GGTGGCCATC
CAGATCCTGC GGAAGAACCC CAAAGGCTTC TTCTTGCTGG TGGAAGGAGG CAGAATTGAC
CACGGGCACC ATGAAGGAAA AGCCAAGCAG GCCCTGCATG AGGCGGTGGA GATGGACCGG
GCCATCGGGC AGGCAGGCAG CTTGACCTCC TCGGAAGACA CTCTGACCGT GGTCACTGCG
GACCATTCCC ACGTCTTCAC ATTTGGTGGA TACACCCCCC GTGGCAACTC TATCTTTGGT
CTGGCCCCCA TGCTGAGTGA CACAGACAAG AAGCCCTTCA CTGCCATCCT GTATGGCAAT
GGGCCTGGCT ACAAGGTGGT GGGCGGTGAA CGAGAGAATG TCTCCATGGT GGACTATGCT
CACAACAACT ACCAGGCGCA GTCTGCTGTG CCCCTGCGCC ACGAGACCCA CGGCGGGGAG
GACGTGGCCG TCTTCTCCAA GGGCCCCATG GCGCACCTGC TGCACGGCGT CCACGAGCAG
AACTACGTCC CCCACGTGAT GGCGTATGCA GCCTGCATCG GGGCCAACCT CGGCCACTGT
GCTCCTGCCA GCTCGGCAGG CAGCCTTGCT GCAGGCCCCC TGCTGCTCGC GCTGGCCCTC
TACCCCCTGA GCGTCCTGTT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu33310
Clone ID Related Accession (Same CDS sequence) XM_006710546.2 , XM_005245818.1
Accession Version XM_006710546.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1575bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product alkaline phosphatase, tissue-nonspecific isozyme isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006710546.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGATTTCAC CATTCTTAGT ACTGGCCATT GGCACCTGCC TTACTAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTACTGGCGA GACCAAGCGC AAGAGACACT GAAATATGCC
CTGGAGCTTC AGAAGCTCAA CACCAACGTG GCTAAGAATG TCATCATGTT CCTGGGAGAT
GGGATGGGTG TCTCCACAGT GACGGCTGCC CGCATCCTCA AGGGTCAGCT CCACCACAAC
CCTGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCCT TCGTGGCCCT CTCCAAGACG
TACAACACCA ATGCCCAGGT CCCTGACAGT GCCGGCACCG CCACCGCCTA CCTGTGTGGG
GTGAAGGCCA ATGAGGGCAC CGTGGGGGTA AGCGCAGCCA CTGAGCGTTC CCGGTGCAAC
ACCACCCAGG GGAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT
GTGGGCATTG TGACCACCAC GAGAGTGAAC CATGCCACCC CCAGCGCCGC CTACGCCCAC
TCGGCTGACC GGGACTGGTA CTCAGACAAC GAGATGCCCC CTGAGGCCTT GAGCCAGGGC
TGTAAGGACA TCGCCTACCA GCTCATGCAT AACATCAGGG ACATTGACGT GATCATGGGG
GGTGGCCGGA AATACATGTA CCCCAAGAAT AAAACTGATG TGGAGTATGA GAGTGACGAG
AAAGCCAGGG GCACGAGGCT GGACGGCCTG GACCTCGTTG ACACCTGGAA GAGCTTCAAA
CCGAGATACA AGCACTCCCA CTTCATCTGG AACCGCACGG AACTCCTGAC CCTTGACCCC
CACAATGTGG ACTACCTATT GGGTCTCTTC GAGCCAGGGG ACATGCAGTA CGAGCTGAAC
AGGAACAACG TGACGGACCC GTCACTCTCC GAGATGGTGG TGGTGGCCAT CCAGATCCTG
CGGAAGAACC CCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGAATTGA CCACGGGCAC
CATGAAGGAA AAGCCAAGCA GGCCCTGCAT GAGGCGGTGG AGATGGACCG GGCCATCGGG
CAGGCAGGCA GCTTGACCTC CTCGGAAGAC ACTCTGACCG TGGTCACTGC GGACCATTCC
CACGTCTTCA CATTTGGTGG ATACACCCCC CGTGGCAACT CTATCTTTGG TCTGGCCCCC
ATGCTGAGTG ACACAGACAA GAAGCCCTTC ACTGCCATCC TGTATGGCAA TGGGCCTGGC
TACAAGGTGG TGGGCGGTGA ACGAGAGAAT GTCTCCATGG TGGACTATGC TCACAACAAC
TACCAGGCGC AGTCTGCTGT GCCCCTGCGC CACGAGACCC ACGGCGGGGA GGACGTGGCC
GTCTTCTCCA AGGGCCCCAT GGCGCACCTG CTGCACGGCG TCCACGAGCA GAACTACGTC
CCCCACGTGA TGGCGTATGC AGCCTGCATC GGGGCCAACC TCGGCCACTG TGCTCCTGCC
AGCTCGGCAG GCAGCCTTGC TGCAGGCCCC CTGCTGCTCG CGCTGGCCCT CTACCCCCTG
AGCGTCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006710609.1
CDS149..1723
Misc Feature(1)299..1618
Misc Feature(2)order(326..328,473..478,665..667,698..700,1142..1144,1157..1159,1169..1171,1280..1285,1508..1510)
Misc Feature(3)order(326..328,473..478,698..700,1157..1159,1169..1171,1283..1285,1508..1510)
Misc Feature(4)order(326..328,476..478,665..667,1142..1144,1157..1159,1169..1171,1280..1285,1508..1510)
Misc Feature(5)order(338..343,350..352,359..364,431..439,443..445,449..451,455..457,467..469,506..514,542..544,587..589,1505..1507,1511..1522,1526..1528,1556..1558,1562..1576,1580..1585,1592..1594)
Translation

Target ORF information:

RefSeq Version XM_006710546.2
Organism Homo sapiens(Human)
Definition Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006710546.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGATTTCAC CATTCTTAGT ACTGGCCATT GGCACCTGCC TTACTAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTACTGGCGA GACCAAGCGC AAGAGACACT GAAATATGCC
CTGGAGCTTC AGAAGCTCAA CACCAACGTG GCTAAGAATG TCATCATGTT CCTGGGAGAT
GGGATGGGTG TCTCCACAGT GACGGCTGCC CGCATCCTCA AGGGTCAGCT CCACCACAAC
CCTGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCCT TCGTGGCCCT CTCCAAGACG
TACAACACCA ATGCCCAGGT CCCTGACAGT GCCGGCACCG CCACCGCCTA CCTGTGTGGG
GTGAAGGCCA ATGAGGGCAC CGTGGGGGTA AGCGCAGCCA CTGAGCGTTC CCGGTGCAAC
ACCACCCAGG GGAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT
GTGGGCATTG TGACCACCAC GAGAGTGAAC CATGCCACCC CCAGCGCCGC CTACGCCCAC
TCGGCTGACC GGGACTGGTA CTCAGACAAC GAGATGCCCC CTGAGGCCTT GAGCCAGGGC
TGTAAGGACA TCGCCTACCA GCTCATGCAT AACATCAGGG ACATTGACGT GATCATGGGG
GGTGGCCGGA AATACATGTA CCCCAAGAAT AAAACTGATG TGGAGTATGA GAGTGACGAG
AAAGCCAGGG GCACGAGGCT GGACGGCCTG GACCTCGTTG ACACCTGGAA GAGCTTCAAA
CCGAGATACA AGCACTCCCA CTTCATCTGG AACCGCACGG AACTCCTGAC CCTTGACCCC
CACAATGTGG ACTACCTATT GGGTCTCTTC GAGCCAGGGG ACATGCAGTA CGAGCTGAAC
AGGAACAACG TGACGGACCC GTCACTCTCC GAGATGGTGG TGGTGGCCAT CCAGATCCTG
CGGAAGAACC CCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGAATTGA CCACGGGCAC
CATGAAGGAA AAGCCAAGCA GGCCCTGCAT GAGGCGGTGG AGATGGACCG GGCCATCGGG
CAGGCAGGCA GCTTGACCTC CTCGGAAGAC ACTCTGACCG TGGTCACTGC GGACCATTCC
CACGTCTTCA CATTTGGTGG ATACACCCCC CGTGGCAACT CTATCTTTGG TCTGGCCCCC
ATGCTGAGTG ACACAGACAA GAAGCCCTTC ACTGCCATCC TGTATGGCAA TGGGCCTGGC
TACAAGGTGG TGGGCGGTGA ACGAGAGAAT GTCTCCATGG TGGACTATGC TCACAACAAC
TACCAGGCGC AGTCTGCTGT GCCCCTGCGC CACGAGACCC ACGGCGGGGA GGACGTGGCC
GTCTTCTCCA AGGGCCCCAT GGCGCACCTG CTGCACGGCG TCCACGAGCA GAACTACGTC
CCCCACGTGA TGGCGTATGC AGCCTGCATC GGGGCCAACC TCGGCCACTG TGCTCCTGCC
AGCTCGGCAG GCAGCCTTGC TGCAGGCCCC CTGCTGCTCG CGCTGGCCCT CTACCCCCTG
AGCGTCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu33310
Clone ID Related Accession (Same CDS sequence) XM_006710546.2 , XM_005245818.1
Accession Version XM_005245818.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1575bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product alkaline phosphatase, tissue-nonspecific isozyme isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGATTTCAC CATTCTTAGT ACTGGCCATT GGCACCTGCC TTACTAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTACTGGCGA GACCAAGCGC AAGAGACACT GAAATATGCC
CTGGAGCTTC AGAAGCTCAA CACCAACGTG GCTAAGAATG TCATCATGTT CCTGGGAGAT
GGGATGGGTG TCTCCACAGT GACGGCTGCC CGCATCCTCA AGGGTCAGCT CCACCACAAC
CCTGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCCT TCGTGGCCCT CTCCAAGACG
TACAACACCA ATGCCCAGGT CCCTGACAGT GCCGGCACCG CCACCGCCTA CCTGTGTGGG
GTGAAGGCCA ATGAGGGCAC CGTGGGGGTA AGCGCAGCCA CTGAGCGTTC CCGGTGCAAC
ACCACCCAGG GGAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT
GTGGGCATTG TGACCACCAC GAGAGTGAAC CATGCCACCC CCAGCGCCGC CTACGCCCAC
TCGGCTGACC GGGACTGGTA CTCAGACAAC GAGATGCCCC CTGAGGCCTT GAGCCAGGGC
TGTAAGGACA TCGCCTACCA GCTCATGCAT AACATCAGGG ACATTGACGT GATCATGGGG
GGTGGCCGGA AATACATGTA CCCCAAGAAT AAAACTGATG TGGAGTATGA GAGTGACGAG
AAAGCCAGGG GCACGAGGCT GGACGGCCTG GACCTCGTTG ACACCTGGAA GAGCTTCAAA
CCGAGATACA AGCACTCCCA CTTCATCTGG AACCGCACGG AACTCCTGAC CCTTGACCCC
CACAATGTGG ACTACCTATT GGGTCTCTTC GAGCCAGGGG ACATGCAGTA CGAGCTGAAC
AGGAACAACG TGACGGACCC GTCACTCTCC GAGATGGTGG TGGTGGCCAT CCAGATCCTG
CGGAAGAACC CCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGAATTGA CCACGGGCAC
CATGAAGGAA AAGCCAAGCA GGCCCTGCAT GAGGCGGTGG AGATGGACCG GGCCATCGGG
CAGGCAGGCA GCTTGACCTC CTCGGAAGAC ACTCTGACCG TGGTCACTGC GGACCATTCC
CACGTCTTCA CATTTGGTGG ATACACCCCC CGTGGCAACT CTATCTTTGG TCTGGCCCCC
ATGCTGAGTG ACACAGACAA GAAGCCCTTC ACTGCCATCC TGTATGGCAA TGGGCCTGGC
TACAAGGTGG TGGGCGGTGA ACGAGAGAAT GTCTCCATGG TGGACTATGC TCACAACAAC
TACCAGGCGC AGTCTGCTGT GCCCCTGCGC CACGAGACCC ACGGCGGGGA GGACGTGGCC
GTCTTCTCCA AGGGCCCCAT GGCGCACCTG CTGCACGGCG TCCACGAGCA GAACTACGTC
CCCCACGTGA TGGCGTATGC AGCCTGCATC GGGGCCAACC TCGGCCACTG TGCTCCTGCC
AGCTCGGCAG GCAGCCTTGC TGCAGGCCCC CTGCTGCTCG CGCTGGCCCT CTACCCCCTG
AGCGTCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005245875.1
CDS235..1809
Misc Feature(1)385..1704
Misc Feature(2)order(412..414,559..564,751..753,784..786,1228..1230,1243..1245,1255..1257,1366..1371,1594..1596)
Misc Feature(3)order(412..414,559..564,784..786,1243..1245,1255..1257,1369..1371,1594..1596)
Misc Feature(4)order(412..414,562..564,751..753,1228..1230,1243..1245,1255..1257,1366..1371,1594..1596)
Misc Feature(5)order(424..429,436..438,445..450,517..525,529..531,535..537,541..543,553..555,592..600,628..630,673..675,1591..1593,1597..1608,1612..1614,1642..1644,1648..1662,1666..1671,1678..1680)
Translation

Target ORF information:

RefSeq Version XM_005245818.1
Organism Homo sapiens(Human)
Definition Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005245818.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGATTTCAC CATTCTTAGT ACTGGCCATT GGCACCTGCC TTACTAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTACTGGCGA GACCAAGCGC AAGAGACACT GAAATATGCC
CTGGAGCTTC AGAAGCTCAA CACCAACGTG GCTAAGAATG TCATCATGTT CCTGGGAGAT
GGGATGGGTG TCTCCACAGT GACGGCTGCC CGCATCCTCA AGGGTCAGCT CCACCACAAC
CCTGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCCT TCGTGGCCCT CTCCAAGACG
TACAACACCA ATGCCCAGGT CCCTGACAGT GCCGGCACCG CCACCGCCTA CCTGTGTGGG
GTGAAGGCCA ATGAGGGCAC CGTGGGGGTA AGCGCAGCCA CTGAGCGTTC CCGGTGCAAC
ACCACCCAGG GGAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT
GTGGGCATTG TGACCACCAC GAGAGTGAAC CATGCCACCC CCAGCGCCGC CTACGCCCAC
TCGGCTGACC GGGACTGGTA CTCAGACAAC GAGATGCCCC CTGAGGCCTT GAGCCAGGGC
TGTAAGGACA TCGCCTACCA GCTCATGCAT AACATCAGGG ACATTGACGT GATCATGGGG
GGTGGCCGGA AATACATGTA CCCCAAGAAT AAAACTGATG TGGAGTATGA GAGTGACGAG
AAAGCCAGGG GCACGAGGCT GGACGGCCTG GACCTCGTTG ACACCTGGAA GAGCTTCAAA
CCGAGATACA AGCACTCCCA CTTCATCTGG AACCGCACGG AACTCCTGAC CCTTGACCCC
CACAATGTGG ACTACCTATT GGGTCTCTTC GAGCCAGGGG ACATGCAGTA CGAGCTGAAC
AGGAACAACG TGACGGACCC GTCACTCTCC GAGATGGTGG TGGTGGCCAT CCAGATCCTG
CGGAAGAACC CCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGAATTGA CCACGGGCAC
CATGAAGGAA AAGCCAAGCA GGCCCTGCAT GAGGCGGTGG AGATGGACCG GGCCATCGGG
CAGGCAGGCA GCTTGACCTC CTCGGAAGAC ACTCTGACCG TGGTCACTGC GGACCATTCC
CACGTCTTCA CATTTGGTGG ATACACCCCC CGTGGCAACT CTATCTTTGG TCTGGCCCCC
ATGCTGAGTG ACACAGACAA GAAGCCCTTC ACTGCCATCC TGTATGGCAA TGGGCCTGGC
TACAAGGTGG TGGGCGGTGA ACGAGAGAAT GTCTCCATGG TGGACTATGC TCACAACAAC
TACCAGGCGC AGTCTGCTGT GCCCCTGCGC CACGAGACCC ACGGCGGGGA GGACGTGGCC
GTCTTCTCCA AGGGCCCCAT GGCGCACCTG CTGCACGGCG TCCACGAGCA GAACTACGTC
CCCCACGTGA TGGCGTATGC AGCCTGCATC GGGGCCAACC TCGGCCACTG TGCTCCTGCC
AGCTCGGCAG GCAGCCTTGC TGCAGGCCCC CTGCTGCTCG CGCTGGCCCT CTACCCCCTG
AGCGTCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu87169
Clone ID Related Accession (Same CDS sequence) XM_017000903.1
Accession Version XM_017000903.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1419bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product alkaline phosphatase, tissue-nonspecific isozyme isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCCCTGGA GCTTCAGAAG CTCAACACCA ACGTGGCTAA GAATGTCATC ATGTTCCTGG 
GAGATGGGTC AGCTCCACCA CAACCCTGGG GAGGAGACCA GGCTGGAGAT GGACAAGTTC
CCCTTCGTGG CCCTCTCCAA GACGTACAAC ACCAATGCCC AGGTCCCTGA CAGTGCCGGC
ACCGCCACCG CCTACCTGTG TGGGGTGAAG GCCAATGAGG GCACCGTGGG GGTAAGCGCA
GCCACTGAGC GTTCCCGGTG CAACACCACC CAGGGGAACG AGGTCACCTC CATCCTGCGC
TGGGCCAAGG ACGCTGGGAA ATCTGTGGGC ATTGTGACCA CCACGAGAGT GAACCATGCC
ACCCCCAGCG CCGCCTACGC CCACTCGGCT GACCGGGACT GGTACTCAGA CAACGAGATG
CCCCCTGAGG CCTTGAGCCA GGGCTGTAAG GACATCGCCT ACCAGCTCAT GCATAACATC
AGGGACATTG ACGTGATCAT GGGGGGTGGC CGGAAATACA TGTACCCCAA GAATAAAACT
GATGTGGAGT ATGAGAGTGA CGAGAAAGCC AGGGGCACGA GGCTGGACGG CCTGGACCTC
GTTGACACCT GGAAGAGCTT CAAACCGAGA TACAAGCACT CCCACTTCAT CTGGAACCGC
ACGGAACTCC TGACCCTTGA CCCCCACAAT GTGGACTACC TATTGGGTCT CTTCGAGCCA
GGGGACATGC AGTACGAGCT GAACAGGAAC AACGTGACGG ACCCGTCACT CTCCGAGATG
GTGGTGGTGG CCATCCAGAT CCTGCGGAAG AACCCCAAAG GCTTCTTCTT GCTGGTGGAA
GGAGGCAGAA TTGACCACGG GCACCATGAA GGAAAAGCCA AGCAGGCCCT GCATGAGGCG
GTGGAGATGG ACCGGGCCAT CGGGCAGGCA GGCAGCTTGA CCTCCTCGGA AGACACTCTG
ACCGTGGTCA CTGCGGACCA TTCCCACGTC TTCACATTTG GTGGATACAC CCCCCGTGGC
AACTCTATCT TTGGTCTGGC CCCCATGCTG AGTGACACAG ACAAGAAGCC CTTCACTGCC
ATCCTGTATG GCAATGGGCC TGGCTACAAG GTGGTGGGCG GTGAACGAGA GAATGTCTCC
ATGGTGGACT ATGCTCACAA CAACTACCAG GCGCAGTCTG CTGTGCCCCT GCGCCACGAG
ACCCACGGCG GGGAGGACGT GGCCGTCTTC TCCAAGGGCC CCATGGCGCA CCTGCTGCAC
GGCGTCCACG AGCAGAACTA CGTCCCCCAC GTGATGGCGT ATGCAGCCTG CATCGGGGCC
AACCTCGGCC ACTGTGCTCC TGCCAGCTCG GCAGGCAGCC TTGCTGCAGG CCCCCTGCTG
CTCGCGCTGG CCCTCTACCC CCTGAGCGTC CTGTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016856392.1
CDS1..1419
Translation

Target ORF information:

RefSeq Version XM_017000903.1
Organism Homo sapiens(Human)
Definition Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017000903.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCCCTGGA GCTTCAGAAG CTCAACACCA ACGTGGCTAA GAATGTCATC ATGTTCCTGG 
GAGATGGGTC AGCTCCACCA CAACCCTGGG GAGGAGACCA GGCTGGAGAT GGACAAGTTC
CCCTTCGTGG CCCTCTCCAA GACGTACAAC ACCAATGCCC AGGTCCCTGA CAGTGCCGGC
ACCGCCACCG CCTACCTGTG TGGGGTGAAG GCCAATGAGG GCACCGTGGG GGTAAGCGCA
GCCACTGAGC GTTCCCGGTG CAACACCACC CAGGGGAACG AGGTCACCTC CATCCTGCGC
TGGGCCAAGG ACGCTGGGAA ATCTGTGGGC ATTGTGACCA CCACGAGAGT GAACCATGCC
ACCCCCAGCG CCGCCTACGC CCACTCGGCT GACCGGGACT GGTACTCAGA CAACGAGATG
CCCCCTGAGG CCTTGAGCCA GGGCTGTAAG GACATCGCCT ACCAGCTCAT GCATAACATC
AGGGACATTG ACGTGATCAT GGGGGGTGGC CGGAAATACA TGTACCCCAA GAATAAAACT
GATGTGGAGT ATGAGAGTGA CGAGAAAGCC AGGGGCACGA GGCTGGACGG CCTGGACCTC
GTTGACACCT GGAAGAGCTT CAAACCGAGA TACAAGCACT CCCACTTCAT CTGGAACCGC
ACGGAACTCC TGACCCTTGA CCCCCACAAT GTGGACTACC TATTGGGTCT CTTCGAGCCA
GGGGACATGC AGTACGAGCT GAACAGGAAC AACGTGACGG ACCCGTCACT CTCCGAGATG
GTGGTGGTGG CCATCCAGAT CCTGCGGAAG AACCCCAAAG GCTTCTTCTT GCTGGTGGAA
GGAGGCAGAA TTGACCACGG GCACCATGAA GGAAAAGCCA AGCAGGCCCT GCATGAGGCG
GTGGAGATGG ACCGGGCCAT CGGGCAGGCA GGCAGCTTGA CCTCCTCGGA AGACACTCTG
ACCGTGGTCA CTGCGGACCA TTCCCACGTC TTCACATTTG GTGGATACAC CCCCCGTGGC
AACTCTATCT TTGGTCTGGC CCCCATGCTG AGTGACACAG ACAAGAAGCC CTTCACTGCC
ATCCTGTATG GCAATGGGCC TGGCTACAAG GTGGTGGGCG GTGAACGAGA GAATGTCTCC
ATGGTGGACT ATGCTCACAA CAACTACCAG GCGCAGTCTG CTGTGCCCCT GCGCCACGAG
ACCCACGGCG GGGAGGACGT GGCCGTCTTC TCCAAGGGCC CCATGGCGCA CCTGCTGCAC
GGCGTCCACG AGCAGAACTA CGTCCCCCAC GTGATGGCGT ATGCAGCCTG CATCGGGGCC
AACCTCGGCC ACTGTGCTCC TGCCAGCTCG GCAGGCAGCC TTGCTGCAGG CCCCCTGCTG
CTCGCGCTGG CCCTCTACCC CCTGAGCGTC CTGTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Human gingiva transcriptome during wound healing.
Journal of clinical periodontology44(4)394-402(2017 Apr)
Wang Y,Tatakis DN


book

Molecular defect of tissue-nonspecific alkaline phosphatase bearing a substitution at position 426 associated with hypophosphatasia.
Molecular and cellular biochemistry427(1-2)169-176(2017 Mar)
Al-Shawafi HA,Komaru K,Oda K


book

Serum Alkaline Phosphatase Levels are Not Associated with Increased Death Risk in Prevalent Hemodialysis Patients: 5-Year Experience in a Single Hemodialysis Center.
Kidney & blood pressure research41(4)498-506(2016)
Zhu JG,Cheng BC,Lee WC,Li LC,Lee CH,Long G,Chen JB


book

Immunofluorescence Labeling of a Mutant of Tissue Non-Specific Alkaline Phosphatase Lacking the Glysosylphosphatidylinositol Anchor.
Journal of Nippon Medical School = Nippon Ika Daigaku zasshi83(4)140-1(2016)
Matsumura T,Saito S,Orimo H


book

The role of phosphatases in the initiation of skeletal mineralization.
Calcified tissue international93(4)299-306(2013 Oct)
Millán JL


 
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