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MFSD8 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol MFSD8
Entrez Gene ID 256471
Full Name major facilitator superfamily domain containing 8
Synonyms CCMD,CLN7
General protein information
Preferred Names

major facilitator superfamily domain containing 8

Names

major facilitator superfamily domain-containing protein 8
ceroid-lipofuscinosis, neuronal 7, late infantile

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

4

4q28.2

Summary This gene encodes a ubiquitous integral membrane protein that contains a transporter domain and a major facilitator superfamily (MFS) domain. Other members of the major facilitator superfamily transport small solutes through chemiosmotic ion gradients. The substrate transported by this protein is unknown. The protein likely localizes to lysosomal membranes. Mutations in this gene are correlated with a variant form of late infantile-onset neuronal ceroid lipofuscinoses (vLINCL). [provided by RefSeq, Oct 2008].
Disorder MIM:

611124

Disorder Html: Ceroid lipofuscinosis, neuronal, 7, 610951 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_152778.2 NP_689991.1 major facilitator superfamily domain-containing protein 8
XM_005262896.1 XP_005262953.1 major facilitator superfamily domain-containing protein 8 isoform X3
XM_005262897.2 XP_005262954.1 major facilitator superfamily domain-containing protein 8 isoform X4
XM_011531832.1 XP_011530134.1 major facilitator superfamily domain-containing protein 8 isoform X7
XM_011531830.1 XP_011530132.1 major facilitator superfamily domain-containing protein 8 isoform X2
XM_005262893.1 XP_005262950.1 major facilitator superfamily domain-containing protein 8 isoform X1
XM_011531831.1 XP_011530133.1 major facilitator superfamily domain-containing protein 8 isoform X5
XM_005262898.2 XP_005262955.1 major facilitator superfamily domain-containing protein 8 isoform X6
XM_017007989.1 XP_016863478.1 major facilitator superfamily domain-containing protein 8 isoform X8



Homo sapiens (human) MFSD8 NP_689991.1
Pan troglodytes (chimpanzee) MFSD8 XP_526685.2
Danio rerio (zebrafish) mfsd8 NP_001038513.1
Caenorhabditis elegans (roundworm) F27D9.2 NP_509296.1
Xenopus tropicalis (tropical clawed frog) mfsd8 XP_002932088.1
Bos taurus (cattle) MFSD8 NP_001192752.1
Mus musculus (house mouse) Mfsd8 NP_082416.2
Caenorhabditis elegans (roundworm) F52F10.2 NP_503443.1
Caenorhabditis elegans (roundworm) srsx-34 NP_505340.1
Caenorhabditis elegans (roundworm) C25E10.5 NP_505338.1
Macaca mulatta (rhesus monkey) LOC713336 XP_002804236.1
Caenorhabditis elegans (roundworm) CELE_ZK550.2 NP_503058.1
Caenorhabditis elegans (roundworm) W05E10.1 NP_505957.1
Caenorhabditis elegans (roundworm) C25E10.4 NP_505339.2
Canis lupus familiaris (dog) MFSD8 XP_533294.3
Gallus gallus (chicken) MFSD8 XP_004941064.1
Drosophila melanogaster (fruit fly) CG8596 NP_648107.3
Caenorhabditis elegans (roundworm) T28C12.1 NP_504617.2
Caenorhabditis elegans (roundworm) F57A8.7 NP_505586.1
Caenorhabditis elegans (roundworm) F58G11.4 NP_001256536.1
Caenorhabditis elegans (roundworm) W03C9.6 NP_496546.2
Anopheles gambiae (African malaria mosquito) AgaP_AGAP012381 XP_320177.4
Caenorhabditis elegans (roundworm) Y53G8AR.7 NP_001022903.1
Caenorhabditis elegans (roundworm) CELE_W01B6.3 NP_501823.2
Caenorhabditis elegans (roundworm) T28C12.2 NP_504616.1
Caenorhabditis elegans (roundworm) CELE_F14D7.6 NP_001256575.1


Related articles in PubMed

Gene disruption of Mfsd8 in mice provides the first animal model for CLN7 disease.
Damme M, Brandenstein L, Fehr S, Jankowiak W, Bartsch U, Schweizer M, Hermans-Borgmeyer I, Storch S
Neurobiology of disease6512-24(2014 May)

Expression and lysosomal targeting of CLN7, a major facilitator superfamily transporter associated with variant late-infantile neuronal ceroid lipofuscinosis.
Sharifi A, Kousi M, Sagné C, Bellenchi GC, Morel L, Darmon M, Hulková H, Ruivo R, Debacker C, El Mestikawy S, Elleder M, Lehesjoki AE, Jalanko A, Gasnier B, Kyttälä A
Human molecular genetics19(22)4497-514(2010 Nov)

Neuronal ceroid lipofuscinosis caused by MFSD8 mutations: a common theme emerging.
Aldahmesh MA, Al-Hassnan ZN, Aldosari M, Alkuraya FS
Neurogenetics10(4)307-11(2009 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study highlights a hierarchy of MFSD8 variant severity, predicting three consequences of mutation: (1) nonsyndromic localized maculopathy, (2) nonsyndromic widespread retinopathy, or (3) syndromic neurological disease.
Title: Specific Alleles of CLN7/MFSD8, a Protein That Localizes to Photoreceptor Synaptic Terminals, Cause a Spectrum of Nonsyndromic Retinal Dystrophy.

In this study, we identified variants in MFSD8 as a novel cause of nonsyndromic autosomal recessive macular dystrophy with central cone involvement.
Title: Mutations in MFSD8, encoding a lysosomal membrane protein, are associated with nonsyndromic autosomal recessive macular dystrophy.

MFSD8 genetic testing should also be considered in patients with Rett like phenotype at onset and negative MECP2 mutation
Title: Rett-like onset in late-infantile neuronal ceroid lipofuscinosis (CLN7) caused by compound heterozygous mutation in the MFSD8 gene and review of the literature data on clinical onset signs.

A mutation in MFSD8, c.472G>A (p.Gly158Ser), segregates with the disease phenotype in variant late infantile neuronal ceroid lipofuscinosis.
Title: Clinico-pathological manifestations of variant late infantile neuronal ceroid lipofuscinosis (vLINCL) caused by a novel mutation in MFSD8 gene.

This study showed that Gene disruption of Mfsd8 provides animal model for CLN7 disease.
Title: Gene disruption of Mfsd8 in mice provides the first animal model for CLN7 disease.

The following MFSD8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MFSD8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu26686
NM_152778.2
Latest version!
Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu26686
XM_005262893.1
Latest version!
Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu38845 XM_005262896.1
Latest version!
Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu38846 XM_005262897.2
Latest version!
Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu38847 XM_005262898.2
Latest version!
Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu78398 XM_011531830.1
Latest version!
Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu78399 XM_011531831.1
Latest version!
Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu78400 XM_011531832.1
Latest version!
Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu95039 XM_017007989.1
Latest version!
Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu26686
Clone ID Related Accession (Same CDS sequence) NM_152778.2 , XM_005262893.1
Accession Version NM_152778.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1557bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-15
Organism Homo sapiens(Human)
Product major facilitator superfamily domain-containing protein 8
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI553701.1, BC029503.1, AA242881.1, CB956202.1, DA481070.1, CX873070.1, CN310788.1 and BQ015234.1. This sequence is a reference standard in the RefSeqGene project. On Sep 11, 2008 this sequence version replaced NM_152778.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC029503.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
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361
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481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGG AAATGTAGCA GTTGTTAGAT CATATACTGC TGGTGCTACT
TCCCTTCAGG AAAGAACAAG TTCCATGGCA AACATAAGCA TGTGTCAAGC ATTAGGTTTT
ATTCTAGGTC CAGTTTTTCA GACTTGTTTT ACATTCCTTG GAGAAAAAGG TGTGACATGG
GATGTGATTA AACTGCAGAT AAACATGTAT ACAACACCAG TTTTACTTAG CGCCTTCCTG
GGAATTTTAA ATATTATTCT GATCCTTGCC ATACTAAGAG AACATCGTGT GGATGACTCA
GGAAGACAGT GTAAAAGTAT TAATTTTGAA GAAGCAAGTA CAGATGAAGC TCAGGTTCCC
CAAGGAAATA TTGACCAGGT TGCTGTTGTG GCCATCAATG TTCTGTTTTT TGTGACTCTA
TTTATCTTTG CCCTTTTTGA AACCATCATT ACTCCATTAA CAATGGATAT GTATGCCTGG
ACTCAAGAAC AAGCTGTGTT ATATAATGGC ATAATACTTG CTGCTCTTGG GGTTGAAGCC
GTTGTTATTT TCTTAGGAGT TAAGTTGCTT TCCAAAAAGA TTGGCGAGCG TGCTATTCTA
CTGGGAGGAC TCATCGTTGT ATGGGTTGGC TTCTTTATCT TGTTACCTTG GGGAAATCAA
TTTCCCAAAA TACAGTGGGA AGATTTGCAC AATAATTCAA TCCCTAATAC CACATTTGGG
GAAATTATTA TTGGTCTTTG GAAGTCTCCA ATGGAAGATG ACAATGAAAG ACCAACTGGT
TGCTCGATTG AACAAGCCTG GTGCCTCTAC ACCCCGGTGA TTCATCTGGC CCAGTTCCTT
ACATCAGCTG TGCTAATAGG ATTAGGCTAT CCAGTCTGCA ATCTTATGTC CTATACTCTA
TATTCAAAAA TTCTAGGACC AAAACCTCAG GGTGTATACA TGGGCTGGTT AACAGCATCT
GGAAGTGGAG CCCGGATTCT TGGGCCTATG TTCATCAGCC AAGTGTATGC TCACTGGGGA
CCACGATGGG CATTCAGCCT GGTGTGTGGA ATAATAGTGC TCACCATCAC CCTCCTGGGA
GTGGTTTACA AAAGACTCAT TGCTCTTTCT GTAAGATATG GGAGGATTCA GGAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_689991.1
CDS164..1720
Misc Feature(1)74..76
Misc Feature(2)200..205
Misc Feature(3)278..1672
Misc Feature(4)284..346
Misc Feature(5)287..1570
Misc Feature(6)order(320..322,329..337,341..346,401..403,410..415,422..424,434..439,443..448,599..604,611..616,623..625,632..634,668..673,680..685,701..703,1001..1003,1010..1015,1022..1027,1034..1036,1076..1078,1100..1102,1112..1114,1121..1123,1133..1135,1442..1444,1451..1456,1463..1465,1475..1480,1487..1489,1520..1525,1532..1537,1544..1549,1556..1558)
Misc Feature(7)386..448
Misc Feature(8)479..541
Misc Feature(9)557..619
Misc Feature(10)683..745
Misc Feature(11)797..859
Misc Feature(12)962..1024
Misc Feature(13)1076..1138
Misc Feature(14)1175..1237
Misc Feature(15)1400..1462
Misc Feature(16)1517..1579
Misc Feature(17)1610..1672
Exon (1)1..88
Exon (2)89..225
Exon (3)226..317
Exon (4)318..361
Exon (5)362..602
Exon (6)603..716
Exon (7)717..861
Exon (8)862..917
Exon (9)918..1026
Exon (10)1027..1161
Exon (11)1162..1265
Exon (12)1266..1513
Exon (13)1514..4545
Translation

Target ORF information:

RefSeq Version NM_152778.2
Organism Homo sapiens(Human)
Definition Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_152778.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGG AAATGTAGCA GTTGTTAGAT CATATACTGC TGGTGCTACT
TCCCTTCAGG AAAGAACAAG TTCCATGGCA AACATAAGCA TGTGTCAAGC ATTAGGTTTT
ATTCTAGGTC CAGTTTTTCA GACTTGTTTT ACATTCCTTG GAGAAAAAGG TGTGACATGG
GATGTGATTA AACTGCAGAT AAACATGTAT ACAACACCAG TTTTACTTAG CGCCTTCCTG
GGAATTTTAA ATATTATTCT GATCCTTGCC ATACTAAGAG AACATCGTGT GGATGACTCA
GGAAGACAGT GTAAAAGTAT TAATTTTGAA GAAGCAAGTA CAGATGAAGC TCAGGTTCCC
CAAGGAAATA TTGACCAGGT TGCTGTTGTG GCCATCAATG TTCTGTTTTT TGTGACTCTA
TTTATCTTTG CCCTTTTTGA AACCATCATT ACTCCATTAA CAATGGATAT GTATGCCTGG
ACTCAAGAAC AAGCTGTGTT ATATAATGGC ATAATACTTG CTGCTCTTGG GGTTGAAGCC
GTTGTTATTT TCTTAGGAGT TAAGTTGCTT TCCAAAAAGA TTGGCGAGCG TGCTATTCTA
CTGGGAGGAC TCATCGTTGT ATGGGTTGGC TTCTTTATCT TGTTACCTTG GGGAAATCAA
TTTCCCAAAA TACAGTGGGA AGATTTGCAC AATAATTCAA TCCCTAATAC CACATTTGGG
GAAATTATTA TTGGTCTTTG GAAGTCTCCA ATGGAAGATG ACAATGAAAG ACCAACTGGT
TGCTCGATTG AACAAGCCTG GTGCCTCTAC ACCCCGGTGA TTCATCTGGC CCAGTTCCTT
ACATCAGCTG TGCTAATAGG ATTAGGCTAT CCAGTCTGCA ATCTTATGTC CTATACTCTA
TATTCAAAAA TTCTAGGACC AAAACCTCAG GGTGTATACA TGGGCTGGTT AACAGCATCT
GGAAGTGGAG CCCGGATTCT TGGGCCTATG TTCATCAGCC AAGTGTATGC TCACTGGGGA
CCACGATGGG CATTCAGCCT GGTGTGTGGA ATAATAGTGC TCACCATCAC CCTCCTGGGA
GTGGTTTACA AAAGACTCAT TGCTCTTTCT GTAAGATATG GGAGGATTCA GGAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26686
Clone ID Related Accession (Same CDS sequence) NM_152778.2 , XM_005262893.1
Accession Version XM_005262893.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1557bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product major facilitator superfamily domain-containing protein 8 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGG AAATGTAGCA GTTGTTAGAT CATATACTGC TGGTGCTACT
TCCCTTCAGG AAAGAACAAG TTCCATGGCA AACATAAGCA TGTGTCAAGC ATTAGGTTTT
ATTCTAGGTC CAGTTTTTCA GACTTGTTTT ACATTCCTTG GAGAAAAAGG TGTGACATGG
GATGTGATTA AACTGCAGAT AAACATGTAT ACAACACCAG TTTTACTTAG CGCCTTCCTG
GGAATTTTAA ATATTATTCT GATCCTTGCC ATACTAAGAG AACATCGTGT GGATGACTCA
GGAAGACAGT GTAAAAGTAT TAATTTTGAA GAAGCAAGTA CAGATGAAGC TCAGGTTCCC
CAAGGAAATA TTGACCAGGT TGCTGTTGTG GCCATCAATG TTCTGTTTTT TGTGACTCTA
TTTATCTTTG CCCTTTTTGA AACCATCATT ACTCCATTAA CAATGGATAT GTATGCCTGG
ACTCAAGAAC AAGCTGTGTT ATATAATGGC ATAATACTTG CTGCTCTTGG GGTTGAAGCC
GTTGTTATTT TCTTAGGAGT TAAGTTGCTT TCCAAAAAGA TTGGCGAGCG TGCTATTCTA
CTGGGAGGAC TCATCGTTGT ATGGGTTGGC TTCTTTATCT TGTTACCTTG GGGAAATCAA
TTTCCCAAAA TACAGTGGGA AGATTTGCAC AATAATTCAA TCCCTAATAC CACATTTGGG
GAAATTATTA TTGGTCTTTG GAAGTCTCCA ATGGAAGATG ACAATGAAAG ACCAACTGGT
TGCTCGATTG AACAAGCCTG GTGCCTCTAC ACCCCGGTGA TTCATCTGGC CCAGTTCCTT
ACATCAGCTG TGCTAATAGG ATTAGGCTAT CCAGTCTGCA ATCTTATGTC CTATACTCTA
TATTCAAAAA TTCTAGGACC AAAACCTCAG GGTGTATACA TGGGCTGGTT AACAGCATCT
GGAAGTGGAG CCCGGATTCT TGGGCCTATG TTCATCAGCC AAGTGTATGC TCACTGGGGA
CCACGATGGG CATTCAGCCT GGTGTGTGGA ATAATAGTGC TCACCATCAC CCTCCTGGGA
GTGGTTTACA AAAGACTCAT TGCTCTTTCT GTAAGATATG GGAGGATTCA GGAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005262950.1
CDS78..1634
Misc Feature(1)192..1586
Misc Feature(2)201..1484
Misc Feature(3)order(234..236,243..251,255..260,315..317,324..329,336..338,348..353,357..362,513..518,525..530,537..539,546..548,582..587,594..599,615..617,915..917,924..929,936..941,948..950,990..992,1014..1016,1026..1028,1035..1037,1047..1049,1356..1358,1365..1370,1377..1379,1389..1394,1401..1403,1434..1439,1446..1451,1458..1463,1470..1472)
Translation

Target ORF information:

RefSeq Version XM_005262893.1
Organism Homo sapiens(Human)
Definition Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005262893.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGG AAATGTAGCA GTTGTTAGAT CATATACTGC TGGTGCTACT
TCCCTTCAGG AAAGAACAAG TTCCATGGCA AACATAAGCA TGTGTCAAGC ATTAGGTTTT
ATTCTAGGTC CAGTTTTTCA GACTTGTTTT ACATTCCTTG GAGAAAAAGG TGTGACATGG
GATGTGATTA AACTGCAGAT AAACATGTAT ACAACACCAG TTTTACTTAG CGCCTTCCTG
GGAATTTTAA ATATTATTCT GATCCTTGCC ATACTAAGAG AACATCGTGT GGATGACTCA
GGAAGACAGT GTAAAAGTAT TAATTTTGAA GAAGCAAGTA CAGATGAAGC TCAGGTTCCC
CAAGGAAATA TTGACCAGGT TGCTGTTGTG GCCATCAATG TTCTGTTTTT TGTGACTCTA
TTTATCTTTG CCCTTTTTGA AACCATCATT ACTCCATTAA CAATGGATAT GTATGCCTGG
ACTCAAGAAC AAGCTGTGTT ATATAATGGC ATAATACTTG CTGCTCTTGG GGTTGAAGCC
GTTGTTATTT TCTTAGGAGT TAAGTTGCTT TCCAAAAAGA TTGGCGAGCG TGCTATTCTA
CTGGGAGGAC TCATCGTTGT ATGGGTTGGC TTCTTTATCT TGTTACCTTG GGGAAATCAA
TTTCCCAAAA TACAGTGGGA AGATTTGCAC AATAATTCAA TCCCTAATAC CACATTTGGG
GAAATTATTA TTGGTCTTTG GAAGTCTCCA ATGGAAGATG ACAATGAAAG ACCAACTGGT
TGCTCGATTG AACAAGCCTG GTGCCTCTAC ACCCCGGTGA TTCATCTGGC CCAGTTCCTT
ACATCAGCTG TGCTAATAGG ATTAGGCTAT CCAGTCTGCA ATCTTATGTC CTATACTCTA
TATTCAAAAA TTCTAGGACC AAAACCTCAG GGTGTATACA TGGGCTGGTT AACAGCATCT
GGAAGTGGAG CCCGGATTCT TGGGCCTATG TTCATCAGCC AAGTGTATGC TCACTGGGGA
CCACGATGGG CATTCAGCCT GGTGTGTGGA ATAATAGTGC TCACCATCAC CCTCCTGGGA
GTGGTTTACA AAAGACTCAT TGCTCTTTCT GTAAGATATG GGAGGATTCA GGAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu38845
Clone ID Related Accession (Same CDS sequence) XM_005262896.1
Accession Version XM_005262896.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1410bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product major facilitator superfamily domain-containing protein 8 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGG AAATGTAGCA GTTGTTAGAT CATATACTGC TGGTGCTACT
TCCCTTCAGG AAAGAACAAG TTCCATGGCA AACATAAGCA TGTGTCAAGC ATTAGGTTTT
ATTCTAGGTC CAGAACATCG TGTGGATGAC TCAGGAAGAC AGTGTAAAAG TATTAATTTT
GAAGAAGCAA GTACAGATGA AGCTCAGGTT CCCCAAGGAA ATATTGACCA GGTTGCTGTT
GTGGCCATCA ATGTTCTGTT TTTTGTGACT CTATTTATCT TTGCCCTTTT TGAAACCATC
ATTACTCCAT TAACAATGGA TATGTATGCC TGGACTCAAG AACAAGCTGT GTTATATAAT
GGCATAATAC TTGCTGCTCT TGGGGTTGAA GCCGTTGTTA TTTTCTTAGG AGTTAAGTTG
CTTTCCAAAA AGATTGGCGA GCGTGCTATT CTACTGGGAG GACTCATCGT TGTATGGGTT
GGCTTCTTTA TCTTGTTACC TTGGGGAAAT CAATTTCCCA AAATACAGTG GGAAGATTTG
CACAATAATT CAATCCCTAA TACCACATTT GGGGAAATTA TTATTGGTCT TTGGAAGTCT
CCAATGGAAG ATGACAATGA AAGACCAACT GGTTGCTCGA TTGAACAAGC CTGGTGCCTC
TACACCCCGG TGATTCATCT GGCCCAGTTC CTTACATCAG CTGTGCTAAT AGGATTAGGC
TATCCAGTCT GCAATCTTAT GTCCTATACT CTATATTCAA AAATTCTAGG ACCAAAACCT
CAGGGTGTAT ACATGGGCTG GTTAACAGCA TCTGGAAGTG GAGCCCGGAT TCTTGGGCCT
ATGTTCATCA GCCAAGTGTA TGCTCACTGG GGACCACGAT GGGCATTCAG CCTGGTGTGT
GGAATAATAG TGCTCACCAT CACCCTCCTG GGAGTGGTTT ACAAAAGACT CATTGCTCTT
TCTGTAAGAT ATGGGAGGAT TCAGGAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005262953.1
CDS78..1487
Misc Feature(1)192..1439
Misc Feature(2)201..1337
Misc Feature(3)order(234..236,243..251,255..260,315..317,324..329,336..338,348..353,357..362,513..518,525..530,537..539,546..548,582..587,594..599,615..617,768..770,777..782,789..794,801..803,843..845,867..869,879..881,888..890,900..902,1209..1211,1218..1223,1230..1232,1242..1247,1254..1256,1287..1292,1299..1304,1311..1316,1323..1325)
Translation

Target ORF information:

RefSeq Version XM_005262896.1
Organism Homo sapiens(Human)
Definition Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005262896.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGG AAATGTAGCA GTTGTTAGAT CATATACTGC TGGTGCTACT
TCCCTTCAGG AAAGAACAAG TTCCATGGCA AACATAAGCA TGTGTCAAGC ATTAGGTTTT
ATTCTAGGTC CAGAACATCG TGTGGATGAC TCAGGAAGAC AGTGTAAAAG TATTAATTTT
GAAGAAGCAA GTACAGATGA AGCTCAGGTT CCCCAAGGAA ATATTGACCA GGTTGCTGTT
GTGGCCATCA ATGTTCTGTT TTTTGTGACT CTATTTATCT TTGCCCTTTT TGAAACCATC
ATTACTCCAT TAACAATGGA TATGTATGCC TGGACTCAAG AACAAGCTGT GTTATATAAT
GGCATAATAC TTGCTGCTCT TGGGGTTGAA GCCGTTGTTA TTTTCTTAGG AGTTAAGTTG
CTTTCCAAAA AGATTGGCGA GCGTGCTATT CTACTGGGAG GACTCATCGT TGTATGGGTT
GGCTTCTTTA TCTTGTTACC TTGGGGAAAT CAATTTCCCA AAATACAGTG GGAAGATTTG
CACAATAATT CAATCCCTAA TACCACATTT GGGGAAATTA TTATTGGTCT TTGGAAGTCT
CCAATGGAAG ATGACAATGA AAGACCAACT GGTTGCTCGA TTGAACAAGC CTGGTGCCTC
TACACCCCGG TGATTCATCT GGCCCAGTTC CTTACATCAG CTGTGCTAAT AGGATTAGGC
TATCCAGTCT GCAATCTTAT GTCCTATACT CTATATTCAA AAATTCTAGG ACCAAAACCT
CAGGGTGTAT ACATGGGCTG GTTAACAGCA TCTGGAAGTG GAGCCCGGAT TCTTGGGCCT
ATGTTCATCA GCCAAGTGTA TGCTCACTGG GGACCACGAT GGGCATTCAG CCTGGTGTGT
GGAATAATAG TGCTCACCAT CACCCTCCTG GGAGTGGTTT ACAAAAGACT CATTGCTCTT
TCTGTAAGAT ATGGGAGGAT TCAGGAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu38846
Clone ID Related Accession (Same CDS sequence) XM_005262897.2
Accession Version XM_005262897.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1356bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product major facilitator superfamily domain-containing protein 8 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005262897.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGG AAATGTAGCA GTTGTTAGAT CATATACTGC TGGTGCTACT
TCCCTTCAGG AAAGAACAAG TTCCATGGCA AACATAAGCA TGTGTCAAGC ATTAGGTTTT
ATTCTAGGTC CAGCAAGTAC AGATGAAGCT CAGGTTCCCC AAGGAAATAT TGACCAGGTT
GCTGTTGTGG CCATCAATGT TCTGTTTTTT GTGACTCTAT TTATCTTTGC CCTTTTTGAA
ACCATCATTA CTCCATTAAC AATGGATATG TATGCCTGGA CTCAAGAACA AGCTGTGTTA
TATAATGGCA TAATACTTGC TGCTCTTGGG GTTGAAGCCG TTGTTATTTT CTTAGGAGTT
AAGTTGCTTT CCAAAAAGAT TGGCGAGCGT GCTATTCTAC TGGGAGGACT CATCGTTGTA
TGGGTTGGCT TCTTTATCTT GTTACCTTGG GGAAATCAAT TTCCCAAAAT ACAGTGGGAA
GATTTGCACA ATAATTCAAT CCCTAATACC ACATTTGGGG AAATTATTAT TGGTCTTTGG
AAGTCTCCAA TGGAAGATGA CAATGAAAGA CCAACTGGTT GCTCGATTGA ACAAGCCTGG
TGCCTCTACA CCCCGGTGAT TCATCTGGCC CAGTTCCTTA CATCAGCTGT GCTAATAGGA
TTAGGCTATC CAGTCTGCAA TCTTATGTCC TATACTCTAT ATTCAAAAAT TCTAGGACCA
AAACCTCAGG GTGTATACAT GGGCTGGTTA ACAGCATCTG GAAGTGGAGC CCGGATTCTT
GGGCCTATGT TCATCAGCCA AGTGTATGCT CACTGGGGAC CACGATGGGC ATTCAGCCTG
GTGTGTGGAA TAATAGTGCT CACCATCACC CTCCTGGGAG TGGTTTACAA AAGACTCATT
GCTCTTTCTG TAAGATATGG GAGGATTCAG GAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005262954.1
CDS78..1433
Misc Feature(1)192..1385
Misc Feature(2)201..1283
Misc Feature(3)order(234..236,243..251,255..260,315..317,324..329,336..338,348..353,357..362,513..518,525..530,537..539,546..548,582..587,594..599,615..617,714..716,723..728,735..740,747..749,789..791,813..815,825..827,834..836,846..848,1155..1157,1164..1169,1176..1178,1188..1193,1200..1202,1233..1238,1245..1250,1257..1262,1269..1271)
Translation

Target ORF information:

RefSeq Version XM_005262897.2
Organism Homo sapiens(Human)
Definition Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005262897.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGG AAATGTAGCA GTTGTTAGAT CATATACTGC TGGTGCTACT
TCCCTTCAGG AAAGAACAAG TTCCATGGCA AACATAAGCA TGTGTCAAGC ATTAGGTTTT
ATTCTAGGTC CAGCAAGTAC AGATGAAGCT CAGGTTCCCC AAGGAAATAT TGACCAGGTT
GCTGTTGTGG CCATCAATGT TCTGTTTTTT GTGACTCTAT TTATCTTTGC CCTTTTTGAA
ACCATCATTA CTCCATTAAC AATGGATATG TATGCCTGGA CTCAAGAACA AGCTGTGTTA
TATAATGGCA TAATACTTGC TGCTCTTGGG GTTGAAGCCG TTGTTATTTT CTTAGGAGTT
AAGTTGCTTT CCAAAAAGAT TGGCGAGCGT GCTATTCTAC TGGGAGGACT CATCGTTGTA
TGGGTTGGCT TCTTTATCTT GTTACCTTGG GGAAATCAAT TTCCCAAAAT ACAGTGGGAA
GATTTGCACA ATAATTCAAT CCCTAATACC ACATTTGGGG AAATTATTAT TGGTCTTTGG
AAGTCTCCAA TGGAAGATGA CAATGAAAGA CCAACTGGTT GCTCGATTGA ACAAGCCTGG
TGCCTCTACA CCCCGGTGAT TCATCTGGCC CAGTTCCTTA CATCAGCTGT GCTAATAGGA
TTAGGCTATC CAGTCTGCAA TCTTATGTCC TATACTCTAT ATTCAAAAAT TCTAGGACCA
AAACCTCAGG GTGTATACAT GGGCTGGTTA ACAGCATCTG GAAGTGGAGC CCGGATTCTT
GGGCCTATGT TCATCAGCCA AGTGTATGCT CACTGGGGAC CACGATGGGC ATTCAGCCTG
GTGTGTGGAA TAATAGTGCT CACCATCACC CTCCTGGGAG TGGTTTACAA AAGACTCATT
GCTCTTTCTG TAAGATATGG GAGGATTCAG GAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu38847
Clone ID Related Accession (Same CDS sequence) XM_005262898.2
Accession Version XM_005262898.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1011bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product major facilitator superfamily domain-containing protein 8 isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 3, 2014 this sequence version replaced XM_005262898.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGG AAATGTAGCA GTTGTTAGAT CATATACTGC TGGTGCTACT
TCCCTTCAGG AAAGAACAAG TTCCATGGCA AACATAAGCA TGTGTCAAGC ATTAGGTTTT
ATTCTAGGTC CAGTTTTTCA GACTTGTTTT ACATTCCTTG GAGAAAAAGG TGTGACATGG
GATGTGATTA AACTGCAGAT AAACATGTAT ACAACACCAG TTTTACTTAG CGCCTTCCTG
GGAATTTTAA ATATTATTCT GATCCTTGCC ATACTAAGAG AACATCGTGT GGATGACTCA
GGAAGACAGT GTAAAAGTAT TAATTTTGAA GAAGCAAGTA CAGATGAAGC TCAGGTTCCC
CAAGGAAATA TTGACCAGGT TGCTGTTGTG GCCATCAATG TTCTGTTTTT TGTGACTCTA
TTTATCTTTG CCCTTTTTGA AACCATCATT ACTCCATTAA CAATGGATAT GTATGCCTGG
ACTCAAGAAC AAGCTGTGTT ATATAATGGC ATAATACTTG CTGCTCTTGG GGTTGAAGCC
GTTGTTATTT TCTTAGGAGT TAAGTTGCTT TCCAAAAAAT TTGCACAATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005262955.1
CDS78..1088
Misc Feature(1)192..>635
Misc Feature(2)201..>635
Translation

Target ORF information:

RefSeq Version XM_005262898.2
Organism Homo sapiens(Human)
Definition Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005262898.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGG AAATGTAGCA GTTGTTAGAT CATATACTGC TGGTGCTACT
TCCCTTCAGG AAAGAACAAG TTCCATGGCA AACATAAGCA TGTGTCAAGC ATTAGGTTTT
ATTCTAGGTC CAGTTTTTCA GACTTGTTTT ACATTCCTTG GAGAAAAAGG TGTGACATGG
GATGTGATTA AACTGCAGAT AAACATGTAT ACAACACCAG TTTTACTTAG CGCCTTCCTG
GGAATTTTAA ATATTATTCT GATCCTTGCC ATACTAAGAG AACATCGTGT GGATGACTCA
GGAAGACAGT GTAAAAGTAT TAATTTTGAA GAAGCAAGTA CAGATGAAGC TCAGGTTCCC
CAAGGAAATA TTGACCAGGT TGCTGTTGTG GCCATCAATG TTCTGTTTTT TGTGACTCTA
TTTATCTTTG CCCTTTTTGA AACCATCATT ACTCCATTAA CAATGGATAT GTATGCCTGG
ACTCAAGAAC AAGCTGTGTT ATATAATGGC ATAATACTTG CTGCTCTTGG GGTTGAAGCC
GTTGTTATTT TCTTAGGAGT TAAGTTGCTT TCCAAAAAAT TTGCACAATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu78398
Clone ID Related Accession (Same CDS sequence) XM_011531830.1
Accession Version XM_011531830.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1443bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product major facilitator superfamily domain-containing protein 8 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGT TTTTCAGACT TGTTTTACAT TCCTTGGAGA AAAAGGTGTG
ACATGGGATG TGATTAAACT GCAGATAAAC ATGTATACAA CACCAGTTTT ACTTAGCGCC
TTCCTGGGAA TTTTAAATAT TATTCTGATC CTTGCCATAC TAAGAGAACA TCGTGTGGAT
GACTCAGGAA GACAGTGTAA AAGTATTAAT TTTGAAGAAG CAAGTACAGA TGAAGCTCAG
GTTCCCCAAG GAAATATTGA CCAGGTTGCT GTTGTGGCCA TCAATGTTCT GTTTTTTGTG
ACTCTATTTA TCTTTGCCCT TTTTGAAACC ATCATTACTC CATTAACAAT GGATATGTAT
GCCTGGACTC AAGAACAAGC TGTGTTATAT AATGGCATAA TACTTGCTGC TCTTGGGGTT
GAAGCCGTTG TTATTTTCTT AGGAGTTAAG TTGCTTTCCA AAAAGATTGG CGAGCGTGCT
ATTCTACTGG GAGGACTCAT CGTTGTATGG GTTGGCTTCT TTATCTTGTT ACCTTGGGGA
AATCAATTTC CCAAAATACA GTGGGAAGAT TTGCACAATA ATTCAATCCC TAATACCACA
TTTGGGGAAA TTATTATTGG TCTTTGGAAG TCTCCAATGG AAGATGACAA TGAAAGACCA
ACTGGTTGCT CGATTGAACA AGCCTGGTGC CTCTACACCC CGGTGATTCA TCTGGCCCAG
TTCCTTACAT CAGCTGTGCT AATAGGATTA GGCTATCCAG TCTGCAATCT TATGTCCTAT
ACTCTATATT CAAAAATTCT AGGACCAAAA CCTCAGGGTG TATACATGGG CTGGTTAACA
GCATCTGGAA GTGGAGCCCG GATTCTTGGG CCTATGTTCA TCAGCCAAGT GTATGCTCAC
TGGGGACCAC GATGGGCATT CAGCCTGGTG TGTGGAATAA TAGTGCTCAC CATCACCCTC
CTGGGAGTGG TTTACAAAAG ACTCATTGCT CTTTCTGTAA GATATGGGAG GATTCAGGAA
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011530132.1
CDS78..1520
Misc Feature(1)192..1472
Misc Feature(2)201..>515
Misc Feature(3)order(234..236,243..251,255..260,315..317,324..329,336..338,348..353,357..362,519..524,531..536,543..545,801..803,810..815,822..827,834..836,876..878,900..902,912..914,921..923,933..935,1242..1244,1251..1256,1263..1265,1275..1280,1287..1289,1320..1325,1332..1337,1344..1349,1356..1358)
Translation

Target ORF information:

RefSeq Version XM_011531830.1
Organism Homo sapiens(Human)
Definition Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011531830.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGT TTTTCAGACT TGTTTTACAT TCCTTGGAGA AAAAGGTGTG
ACATGGGATG TGATTAAACT GCAGATAAAC ATGTATACAA CACCAGTTTT ACTTAGCGCC
TTCCTGGGAA TTTTAAATAT TATTCTGATC CTTGCCATAC TAAGAGAACA TCGTGTGGAT
GACTCAGGAA GACAGTGTAA AAGTATTAAT TTTGAAGAAG CAAGTACAGA TGAAGCTCAG
GTTCCCCAAG GAAATATTGA CCAGGTTGCT GTTGTGGCCA TCAATGTTCT GTTTTTTGTG
ACTCTATTTA TCTTTGCCCT TTTTGAAACC ATCATTACTC CATTAACAAT GGATATGTAT
GCCTGGACTC AAGAACAAGC TGTGTTATAT AATGGCATAA TACTTGCTGC TCTTGGGGTT
GAAGCCGTTG TTATTTTCTT AGGAGTTAAG TTGCTTTCCA AAAAGATTGG CGAGCGTGCT
ATTCTACTGG GAGGACTCAT CGTTGTATGG GTTGGCTTCT TTATCTTGTT ACCTTGGGGA
AATCAATTTC CCAAAATACA GTGGGAAGAT TTGCACAATA ATTCAATCCC TAATACCACA
TTTGGGGAAA TTATTATTGG TCTTTGGAAG TCTCCAATGG AAGATGACAA TGAAAGACCA
ACTGGTTGCT CGATTGAACA AGCCTGGTGC CTCTACACCC CGGTGATTCA TCTGGCCCAG
TTCCTTACAT CAGCTGTGCT AATAGGATTA GGCTATCCAG TCTGCAATCT TATGTCCTAT
ACTCTATATT CAAAAATTCT AGGACCAAAA CCTCAGGGTG TATACATGGG CTGGTTAACA
GCATCTGGAA GTGGAGCCCG GATTCTTGGG CCTATGTTCA TCAGCCAAGT GTATGCTCAC
TGGGGACCAC GATGGGCATT CAGCCTGGTG TGTGGAATAA TAGTGCTCAC CATCACCCTC
CTGGGAGTGG TTTACAAAAG ACTCATTGCT CTTTCTGTAA GATATGGGAG GATTCAGGAA
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu78399
Clone ID Related Accession (Same CDS sequence) XM_011531831.1
Accession Version XM_011531831.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1242bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product major facilitator superfamily domain-containing protein 8 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGC AAGTACAGAT GAAGCTCAGG TTCCCCAAGG AAATATTGAC
CAGGTTGCTG TTGTGGCCAT CAATGTTCTG TTTTTTGTGA CTCTATTTAT CTTTGCCCTT
TTTGAAACCA TCATTACTCC ATTAACAATG GATATGTATG CCTGGACTCA AGAACAAGCT
GTGTTATATA ATGGCATAAT ACTTGCTGCT CTTGGGGTTG AAGCCGTTGT TATTTTCTTA
GGAGTTAAGT TGCTTTCCAA AAAGATTGGC GAGCGTGCTA TTCTACTGGG AGGACTCATC
GTTGTATGGG TTGGCTTCTT TATCTTGTTA CCTTGGGGAA ATCAATTTCC CAAAATACAG
TGGGAAGATT TGCACAATAA TTCAATCCCT AATACCACAT TTGGGGAAAT TATTATTGGT
CTTTGGAAGT CTCCAATGGA AGATGACAAT GAAAGACCAA CTGGTTGCTC GATTGAACAA
GCCTGGTGCC TCTACACCCC GGTGATTCAT CTGGCCCAGT TCCTTACATC AGCTGTGCTA
ATAGGATTAG GCTATCCAGT CTGCAATCTT ATGTCCTATA CTCTATATTC AAAAATTCTA
GGACCAAAAC CTCAGGGTGT ATACATGGGC TGGTTAACAG CATCTGGAAG TGGAGCCCGG
ATTCTTGGGC CTATGTTCAT CAGCCAAGTG TATGCTCACT GGGGACCACG ATGGGCATTC
AGCCTGGTGT GTGGAATAAT AGTGCTCACC ATCACCCTCC TGGGAGTGGT TTACAAAAGA
CTCATTGCTC TTTCTGTAAG ATATGGGAGG ATTCAGGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011530133.1
CDS78..1319
Misc Feature(1)192..1271
Misc Feature(2)201..1169
Misc Feature(3)order(234..236,243..251,255..260,315..317,324..329,336..338,348..353,357..362,513..518,531..536,543..548,555..557,579..584,591..596,612..614,804..806,813..818,825..830,837..839,879..881,891..893,903..905,918..920,1041..1043,1050..1055,1062..1064,1074..1079,1086..1088,1119..1124,1131..1136,1143..1148,1155..1157)
Translation

Target ORF information:

RefSeq Version XM_011531831.1
Organism Homo sapiens(Human)
Definition Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011531831.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGC AAGTACAGAT GAAGCTCAGG TTCCCCAAGG AAATATTGAC
CAGGTTGCTG TTGTGGCCAT CAATGTTCTG TTTTTTGTGA CTCTATTTAT CTTTGCCCTT
TTTGAAACCA TCATTACTCC ATTAACAATG GATATGTATG CCTGGACTCA AGAACAAGCT
GTGTTATATA ATGGCATAAT ACTTGCTGCT CTTGGGGTTG AAGCCGTTGT TATTTTCTTA
GGAGTTAAGT TGCTTTCCAA AAAGATTGGC GAGCGTGCTA TTCTACTGGG AGGACTCATC
GTTGTATGGG TTGGCTTCTT TATCTTGTTA CCTTGGGGAA ATCAATTTCC CAAAATACAG
TGGGAAGATT TGCACAATAA TTCAATCCCT AATACCACAT TTGGGGAAAT TATTATTGGT
CTTTGGAAGT CTCCAATGGA AGATGACAAT GAAAGACCAA CTGGTTGCTC GATTGAACAA
GCCTGGTGCC TCTACACCCC GGTGATTCAT CTGGCCCAGT TCCTTACATC AGCTGTGCTA
ATAGGATTAG GCTATCCAGT CTGCAATCTT ATGTCCTATA CTCTATATTC AAAAATTCTA
GGACCAAAAC CTCAGGGTGT ATACATGGGC TGGTTAACAG CATCTGGAAG TGGAGCCCGG
ATTCTTGGGC CTATGTTCAT CAGCCAAGTG TATGCTCACT GGGGACCACG ATGGGCATTC
AGCCTGGTGT GTGGAATAAT AGTGCTCACC ATCACCCTCC TGGGAGTGGT TTACAAAAGA
CTCATTGCTC TTTCTGTAAG ATATGGGAGG ATTCAGGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu78400
Clone ID Related Accession (Same CDS sequence) XM_011531832.1
Accession Version XM_011531832.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 897bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product major facilitator superfamily domain-containing protein 8 isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGT TTTTCAGACT TGTTTTACAT TCCTTGGAGA AAAAGGTGTG
ACATGGGATG TGATTAAACT GCAGATAAAC ATGTATACAA CACCAGTTTT ACTTAGCGCC
TTCCTGGGAA TTTTAAATAT TATTCTGATC CTTGCCATAC TAAGAGAACA TCGTGTGGAT
GACTCAGGAA GACAGTGTAA AAGTATTAAT TTTGAAGAAG CAAGTACAGA TGAAGCTCAG
GTTCCCCAAG GAAATATTGA CCAGGTTGCT GTTGTGGCCA TCAATGTTCT GTTTTTTGTG
ACTCTATTTA TCTTTGCCCT TTTTGAAACC ATCATTACTC CATTAACAAT GGATATGTAT
GCCTGGACTC AAGAACAAGC TGTGTTATAT AATGGCATAA TACTTGCTGC TCTTGGGGTT
GAAGCCGTTG TTATTTTCTT AGGAGTTAAG TTGCTTTCCA AAAAATTTGC ACAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011530134.1
CDS78..974
Misc Feature(1)192..>548
Misc Feature(2)201..>515
Translation

Target ORF information:

RefSeq Version XM_011531832.1
Organism Homo sapiens(Human)
Definition Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011531832.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGT TTTTCAGACT TGTTTTACAT TCCTTGGAGA AAAAGGTGTG
ACATGGGATG TGATTAAACT GCAGATAAAC ATGTATACAA CACCAGTTTT ACTTAGCGCC
TTCCTGGGAA TTTTAAATAT TATTCTGATC CTTGCCATAC TAAGAGAACA TCGTGTGGAT
GACTCAGGAA GACAGTGTAA AAGTATTAAT TTTGAAGAAG CAAGTACAGA TGAAGCTCAG
GTTCCCCAAG GAAATATTGA CCAGGTTGCT GTTGTGGCCA TCAATGTTCT GTTTTTTGTG
ACTCTATTTA TCTTTGCCCT TTTTGAAACC ATCATTACTC CATTAACAAT GGATATGTAT
GCCTGGACTC AAGAACAAGC TGTGTTATAT AATGGCATAA TACTTGCTGC TCTTGGGGTT
GAAGCCGTTG TTATTTTCTT AGGAGTTAAG TTGCTTTCCA AAAAATTTGC ACAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu95039
Clone ID Related Accession (Same CDS sequence) XM_017007989.1
Accession Version XM_017007989.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 810bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product major facilitator superfamily domain-containing protein 8 isoform X8
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGG AAATGTAGCA GTTGTTAGAT CATATACTGC TGGTGCTACT
TCCCTTCAGG AAAGAACAAG TTCCATGGCA AACATAAGCA TGTGTCAAGC ATTAGGTTTT
ATTCTAGGTC CAGCAAGTAC AGATGAAGCT CAGGTTCCCC AAGGAAATAT TGACCAGGTT
GCTGTTGTGG CCATCAATGT TCTGTTTTTT GTGACTCTAT TTATCTTTGC CCTTTTTGAA
ACCATCATTA CTCCATTAAC AATGGATATG TATGCCTGGA CTCAAGAACA AGCTGTGTTA
TATAATGGCA TAATACTTGC TGCTCTTGGG GTTGAAGCCG TTGTTATTTT CTTAGGAGTT
AAGTTGCTTT CCAAAAAATT TGCACAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016863478.1
CDS78..887
Translation

Target ORF information:

RefSeq Version XM_017007989.1
Organism Homo sapiens(Human)
Definition Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017007989.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGG AAATGTAGCA GTTGTTAGAT CATATACTGC TGGTGCTACT
TCCCTTCAGG AAAGAACAAG TTCCATGGCA AACATAAGCA TGTGTCAAGC ATTAGGTTTT
ATTCTAGGTC CAGCAAGTAC AGATGAAGCT CAGGTTCCCC AAGGAAATAT TGACCAGGTT
GCTGTTGTGG CCATCAATGT TCTGTTTTTT GTGACTCTAT TTATCTTTGC CCTTTTTGAA
ACCATCATTA CTCCATTAAC AATGGATATG TATGCCTGGA CTCAAGAACA AGCTGTGTTA
TATAATGGCA TAATACTTGC TGCTCTTGGG GTTGAAGCCG TTGTTATTTT CTTAGGAGTT
AAGTTGCTTT CCAAAAAATT TGCACAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Gene disruption of Mfsd8 in mice provides the first animal model for CLN7 disease.
Neurobiology of disease6512-24(2014 May)
Damme M,Brandenstein L,Fehr S,Jankowiak W,Bartsch U,Schweizer M,Hermans-Borgmeyer I,Storch S


book

Expression and lysosomal targeting of CLN7, a major facilitator superfamily transporter associated with variant late-infantile neuronal ceroid lipofuscinosis.
Human molecular genetics19(22)4497-514(2010 Nov)
Sharifi A,Kousi M,Sagné C,Bellenchi GC,Morel L,Darmon M,Hulková H,Ruivo R,Debacker C,El Mestikawy S,Elleder M,Lehesjoki AE,Jalanko A,Gasnier B,Kyttälä A


book

Neuronal ceroid lipofuscinosis caused by MFSD8 mutations: a common theme emerging.
Neurogenetics10(4)307-11(2009 Oct)
Aldahmesh MA,Al-Hassnan ZN,Aldosari M,Alkuraya FS


 
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