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GAK cDNA ORF clone, Homo sapiens(Human)

Gene Symbol GAK
Entrez Gene ID 2580
Full Name cyclin G associated kinase
Synonyms DNAJ26,DNAJC26
General protein information
Preferred Names

cyclin G associated kinase

Names

cyclin-G-associated kinase
auxilin-2

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

4

4p16.3

Summary In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015].
Disorder MIM:

602052


mRNA and Protein(s)

mRNA Protein Name
NM_001318134.1 NP_001305063.1 cyclin-G-associated kinase isoform 3
XM_017007991.1 XP_016863480.1 cyclin-G-associated kinase isoform X7
XM_011513430.1 XP_011511732.1 cyclin-G-associated kinase isoform X8
NM_005255.3 NP_005246.2 cyclin-G-associated kinase isoform 1
XM_011513427.2 XP_011511729.1 cyclin-G-associated kinase isoform X3
XM_011513428.2 XP_011511730.1 cyclin-G-associated kinase isoform X4
XM_017007992.1 XP_016863481.1 cyclin-G-associated kinase isoform X11
XM_017007993.1 XP_016863482.1 cyclin-G-associated kinase isoform X13
XM_011513425.2 XP_011511727.1 cyclin-G-associated kinase isoform X1
XM_011513426.2 XP_011511728.1 cyclin-G-associated kinase isoform X2
XM_011513429.2 XP_011511731.1 cyclin-G-associated kinase isoform X6
XM_017007994.1 XP_016863483.1 cyclin-G-associated kinase isoform X14
XM_017007995.1 XP_016863484.1 cyclin-G-associated kinase isoform X15
XM_005272268.2 XP_005272325.1 cyclin-G-associated kinase isoform X5
XM_011513432.2 XP_011511734.1 cyclin-G-associated kinase isoform X10
XM_005272270.2 XP_005272327.1 cyclin-G-associated kinase isoform X12
XM_011513431.2 XP_011511733.1 cyclin-G-associated kinase isoform X9
XM_011513434.2 XP_011511736.1 cyclin-G-associated kinase isoform X13



Rattus norvegicus (Norway rat) Gak NP_112292.1
Homo sapiens (human) GAK NP_005246.2
Bos taurus (cattle) GAK NP_001039549.1
Danio rerio (zebrafish) gak XP_003198642.2
Pan troglodytes (chimpanzee) GAK XP_001140835.1
Mus musculus (house mouse) Gak NP_705797.1
Gallus gallus (chicken) GAK XP_424873.4
Anopheles gambiae (African malaria mosquito) AgaP_AGAP003715 XP_310244.7
Canis lupus familiaris (dog) GAK XP_536279.4
Drosophila melanogaster (fruit fly) aux NP_001262243.1
Macaca mulatta (rhesus monkey) GAK XP_001094120.2
Danio rerio (zebrafish) LOC100151158 XP_001919224.1
Xenopus tropicalis (tropical clawed frog) gak NP_001016350.2


Related articles in PubMed

Genome-wide haplotype association analysis identifies SERPINB9, SERPINE2, GAK, and HSP90B1 as novel risk genes for oral squamous cell carcinoma.
Sun W, Lv W, Lv H, Zhang R, Jiang Y
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(2)1845-51(2016 Feb)

The clathrin-binding and J-domains of GAK support the uncoating and chaperoning of clathrin by Hsc70 in the brain.
Park BC, Yim YI, Zhao X, Olszewski MB, Eisenberg E, Greene LE
Journal of cell science128(20)3811-21(2015 Oct)

Quantitative assessment of the association between GAK rs1564282 C/T polymorphism and the risk of Parkinson's disease.
Ma ZG, He F, Xu J
Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia22(7)1077-80(2015 Jul)

Impact of Parkinson's disease risk loci on age at onset.
Lill CM, Hansen J, Olsen JH, Binder H, Ritz B, Bertram L
Movement disorders : official journal of the Movement Disorder Society30(6)847-50(2015 May)

AP-2-associated protein kinase 1 and cyclin G-associated kinase regulate hepatitis C virus entry and are potential drug targets.
Neveu G, Ziv-Av A, Barouch-Bentov R, Berkerman E, Mulholland J, Einav S
Journal of virology89(8)4387-404(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that the c-Src_GAK_MCM axis plays an important role in cell cycle progression through control of the DNA replication licensing system.
Title: GAK is phosphorylated by c-Src and translocated from the centrosome to chromatin at the end of telophase.

We filtered four OSCC genes including SERPINB9, SERPINE2, GAK, and HSP90B1 through the gene global prioritization score (P < 0.005).
Title: Genome-wide haplotype association analysis identifies SERPINB9, SERPINE2, GAK, and HSP90B1 as novel risk genes for oral squamous cell carcinoma.

GAK gene expression is increased in Parkinson disease
Title: The 4p16.3 Parkinson Disease Risk Locus Is Associated with GAK Expression and Genes Involved with the Synaptic Vesicle Membrane.

This meta-analysis suggests that GAK rs1564282 C/T polymorphism is associated with increased susceptibility to Parkinson's disease--{REVIEW}
Title: Quantitative assessment of the association between GAK rs1564282 C/T polymorphism and the risk of Parkinson's disease.

Data indicated that GBA and TMEM175/GAK significantly alter age at onset in PD.
Title: Impact of Parkinson's disease risk loci on age at onset.

The following GAK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GAK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu95041 XM_017007991.1
Latest version!
Homo sapiens cyclin G associated kinase (GAK), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OHu95042 XM_017007992.1
Latest version!
Homo sapiens cyclin G associated kinase (GAK), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OHu95043 XM_017007994.1
Latest version!
Homo sapiens cyclin G associated kinase (GAK), transcript variant X15, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OHu95044 XM_017007995.1
Latest version!
Homo sapiens cyclin G associated kinase (GAK), transcript variant X16, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $559.30
$799.00
OHu24069 NM_005255.3
Latest version!
Homo sapiens cyclin G associated kinase (GAK), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $923.30
$1319.00
OHu57911 NM_001318134.1
Latest version!
Homo sapiens cyclin G associated kinase (GAK), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu38320 XM_005272268.2
Latest version!
Homo sapiens cyclin G associated kinase (GAK), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu38323 XM_005272270.2
Latest version!
Homo sapiens cyclin G associated kinase (GAK), transcript variant X12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu57905 XM_011513427.2
Latest version!
Homo sapiens cyclin G associated kinase (GAK), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu57906 XM_011513428.2
Latest version!
Homo sapiens cyclin G associated kinase (GAK), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu57907 XM_011513429.2
Latest version!
Homo sapiens cyclin G associated kinase (GAK), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu57908 XM_011513430.1
Latest version!
Homo sapiens cyclin G associated kinase (GAK), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu57909 XM_011513431.2
Latest version!
Homo sapiens cyclin G associated kinase (GAK), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu57910 XM_011513432.2
Latest version!
Homo sapiens cyclin G associated kinase (GAK), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu57912 XM_017007993.1
Latest version!
Homo sapiens cyclin G associated kinase (GAK), transcript variant X14, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu57912 XM_011513434.2
Latest version!
Homo sapiens cyclin G associated kinase (GAK), transcript variant X13, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu57903 XM_011513425.2
Latest version!
Homo sapiens cyclin G associated kinase (GAK), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu57904 XM_011513426.2
Latest version!
Homo sapiens cyclin G associated kinase (GAK), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu95041
Clone ID Related Accession (Same CDS sequence) XM_017007991.1
Accession Version XM_017007991.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3846bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product cyclin-G-associated kinase isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATGTG
GGGAGTGGCA GAGAGTATGC ATTAAAGAGG CTATTATCCA ATGAAGAGGA AAAGAACAGA
GCCATCATTC AAGAAGTTTG CTTCATGAAA AAGCTTTCCG GCCACCCGAA CATTGTCCAG
TTTTGTTCTG CAGCGTCTAT AGGAAAAGAG GAGTCAGACA CGGGGCAGGC TGAGTTCCTC
TTGCTCACAG AGCTCTGTAA AGGGCAGCTG GTGGAATTTT TGAAGAAAAT GGAATCTCGA
GGCCCCCTTT CGTGCGACAC GGTTCTGAAG ATCTTCTACC AGACGTGCCG CGCCGTGCAG
CACATGCACC GGCAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA GAACTTGTTG
CTTAGTAACC AAGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACGAC CATCTCGCAC
TACCCTGACT ACAGCTGGAG CGCCCAGAGG CGAGCCCTGG TGGAGGAAGA GGCCCTGGGC
TGCATCTTGT ACCTGCTGTG CTTCCGGCAG CACCCTTTTG AGGATGGAGC GAAACTTCGA
ATAGTCAATG GGAAGTACTC GATCCCCCCG CACGACACGC AGTACACGGT CTTCCACAGC
CTCATCCGCG CCATGCTGCA GGTGAACCCG GAGGAGCGGC TGTCCATCGC CGAGGTGGTG
CACCAGCTGC AGGAGATCGC GGCCGCCCGC AACGTGAACC CCAAGTCTCC CATCACAGAG
CTCCTGGAGC AGAATGGAGG CTACGGGAGC GCCACACTGT CCCGAGGGCC ACCCCCTCCC
GTGGGCCCCG CTGGCAGTGG CTACAGTGGA GGCCTGGCGC TGGCGGAGTA CGACCAGCCG
TATGGCGGCT TCCTGGACAT TCTGCGGGGT GGGACAGAGC GGCTCTTCAC CAACCTCAAG
GACACCTCCT CCAAGGTCAT CCAGTCCGTC GCTAATTATG CAAAGGGTGA CCTGGACATA
TCTTACATCA CATCCAGAAT TGCAGTGATG TCATTCCCAG CAGAAGGTGT GGAGTCAGCG
CTCAAAAACA ACATCGAAGA TGTGCGGTTG TTCCTGGACT CCAAGCACCC AGGGCACTAT
GCCGTCTACA ACCTGTCCCC GAGGACCTAC CGGCCCTCCA GGTTCCACAA CCGGGTCTCC
GAGTGTGGCT GGGCAGCACG GCGGGCCCCA CACCTGCACA CCCTGTACAA CATCTGCAGG
AACATGCACG CCTGGCTGCG GCAGGACCAC AAGAACGTCT GCGTCGTGCA CTGCATGGAC
GGGAGAGCCG CGTCTGCTGT GGCCGTCTGC TCCTTCCTGT GCTTCTGCCG TCTCTTCAGC
ACCGCGGAGG CCGCCGTGTA CATGTTCAGC ATGAAGCGCT GCCCACCAGG CATCTGGCCA
TCCCACAAAA GGTACATCGA GTACATGTGT GACATGGTGG CGGAGGAGCC CATCACACCC
CACAGCAAGC CCATCCTGGT GAGGGCCGTG GTCATGACAC CCGTGCCGCT GTTCAGCAAG
CAGAGGAGCG GCTGCAGGCC CTTCTGCGAG GTCTACGTGG GGGACGAGCG TGTGGCCAGC
ACCTCCCAGG AGTACGACAA GATGCGGGAC TTTAAGATTG AAGATGGCAA AGCGGTGATT
CCCCTGGGCG TCACGGTGCA AGGAGACGTG CTCATCGTCA TCTATCACGC CCGGTCCACT
CTGGGCGGCC GGCTGCAGGC CAAGATGGCA TCCATGAAGA TGTTCCAGAT TCAGTTCCAC
ACGGGGTTTG TGCCTCGGAA CGCCACCACT GTGAAATTTG CCAAGTATGA CCTGGACGCG
TGTGACATTC AAGAAAAATA CCCGGATTTA TTTCAAGTGA ACCTGGAAGT GGAGGTGGAG
CCCAGGGACA GGCCGAGCCG GGAAGCCCCA CCATGGGAGA ACTCGAGCAT GAGGGGGCTG
AACCCCAAAA TCCTGTTTTC CAGCCGGGAG GAGCAGCAAG ACATTCTGTC TAAGTTTGGG
AAGCCGGAGC TTCCCCGGCA GCCTGGCTCC ACGGCTCAGT ATGATGCTGG GGCAGGGTCC
CCGGAAGCCG AACCCACAGA CTCTGACTCA CCGCCAAGCA GCAGCGCGGA CGCCAGTCGC
TTCCTGCACA CGCTGGACTG GCAGGAAGAG AAGGAGGCAG AGACTGGTGC AGAAAATGCC
TCTTCCAAGG AGAGCGAGTC TGCCCTGATG GAGGACAGAG ACGAGAGTGA GGTGTCAGAT
GAAGGGGGAT CCCCGATCTC CAGCGAGGGC CAGGAACCCA GGGCCGACCC AGAGCCCCCC
GGCCTGGCAG CAGGGCTGGT GCAGCAGGAC TTGGTTTTTG AGGTGGAGAC ACCGGCTGTG
CTGCCAGAGC CTGTGCCACA GGAAGACGGG GTCGACCTCC TGGGCCTGCA CTCCGAGGTG
GGCGCAGGGC CAGCTGTACC CCCGCAGGCC TGCAAGGCCC CCTCCAGCAA CACCGACCTG
CTCAGCTGCC TCCTTGGGCC CCCTGAGGCC GCCTCCCAGG GGCCCCCGGA GGATCTGCTC
AGCGAGGACC CGCTGCTCCT GGCAAGCCCG GCCCCTCCCC TGAGCGTGCA GAGCACCCCA
AGAGGAGGGC CCCCTGCCGC TGCTGACCCC TTTGGCCCGC TTCTGCCGTC TTCAGGCAAC
AACTCCCAGC CCTGCTCCAA TCCTGATCTC TTCGGCGAAT TTCTCAATTC GGACTCTGTG
ACCGTCCCAC CATCCTTCCC GTCTGCCCAC AGTGCTCCGC CCCCATCCTG CAGCGCCGAC
TTCCTGCACC TGGGGGATCT GCCAGGAGAG CCCAGCAAGA TGACAGCCTC GTCCAGCAAC
CCAGACCTGC TGGGAGGATG GGCTGCCTGG ACCGAGACTG CAGCATCGGC AGTGGCCCCC
ACGCCAGCCA CAGAAGGCCC CCTCTTCTCT CCTGGAGGTC AGCCGGCCCC TTGTGGCTCT
CAGGCCAGCT GGACCAAGTC TCAGAACCCG GACCCATTTG CTGACCTTGG CGACCTCAGC
TCCGGCCTCC AAGGCTCACC AGCTGGATTC CCTCCTGGGG GCTTCATTCC CAAAACGGCC
ACCACGCCCA AAGGCAGCAG CTCCTGGCAG ACAAGTCGGC CGCCAGCCCA GGGCGCCTCA
TGGCCCCCTC AGGCCAAGCC GCCCCCCAAA GCCTGCACAC AGCCAAGGCC TAACTATGCC
TCGAACTTCA GTGTGATCGG GGCGCGGGAG GAGCGGGGGG TCCGCGCACC CAGCTTTGCT
CAAAAGCCAA AAGTCTCTGA GAACGACTTT GAAGATCTGT TGTCCAATCA AGGCTTCTCC
TCCAGGTCTG ACAAGAAAGG GCCAAAGACC ATTGCAGAGA TGAGGAAGCA GGACCTGGCT
AAAGACACGG ACCCACTCAA GCTGAAGCTC CTGGACTGGA TTGAGGGCAA GGAGCGGAAC
ATCCGGGCCC TGCTGTCCAC GCTGCACACA GTGCTGTGGG ACGGGGAGAG CCGCTGGACG
CCCGTGGGCA TGGCCGACCT GGTGGCTCCG GAGCAAGTGA AGAAGCACTA TCGCCGCGCG
GTGCTGGCTG TGCACCCCGA CAAGGCTGCG GGGCAGCCGT ACGAGCAGCA CGCCAAGATG
ATCTTCATGG AGCTGAATGA CGCCTGGTCG GAGTTTGAGA ACCAGGGCTC CCGGCCCCTC
TTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016863480.1
CDS144..3989
Translation

Target ORF information:

RefSeq Version XM_017007991.1
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin G associated kinase (GAK), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017007991.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATGTG
GGGAGTGGCA GAGAGTATGC ATTAAAGAGG CTATTATCCA ATGAAGAGGA AAAGAACAGA
GCCATCATTC AAGAAGTTTG CTTCATGAAA AAGCTTTCCG GCCACCCGAA CATTGTCCAG
TTTTGTTCTG CAGCGTCTAT AGGAAAAGAG GAGTCAGACA CGGGGCAGGC TGAGTTCCTC
TTGCTCACAG AGCTCTGTAA AGGGCAGCTG GTGGAATTTT TGAAGAAAAT GGAATCTCGA
GGCCCCCTTT CGTGCGACAC GGTTCTGAAG ATCTTCTACC AGACGTGCCG CGCCGTGCAG
CACATGCACC GGCAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA GAACTTGTTG
CTTAGTAACC AAGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACGAC CATCTCGCAC
TACCCTGACT ACAGCTGGAG CGCCCAGAGG CGAGCCCTGG TGGAGGAAGA GGCCCTGGGC
TGCATCTTGT ACCTGCTGTG CTTCCGGCAG CACCCTTTTG AGGATGGAGC GAAACTTCGA
ATAGTCAATG GGAAGTACTC GATCCCCCCG CACGACACGC AGTACACGGT CTTCCACAGC
CTCATCCGCG CCATGCTGCA GGTGAACCCG GAGGAGCGGC TGTCCATCGC CGAGGTGGTG
CACCAGCTGC AGGAGATCGC GGCCGCCCGC AACGTGAACC CCAAGTCTCC CATCACAGAG
CTCCTGGAGC AGAATGGAGG CTACGGGAGC GCCACACTGT CCCGAGGGCC ACCCCCTCCC
GTGGGCCCCG CTGGCAGTGG CTACAGTGGA GGCCTGGCGC TGGCGGAGTA CGACCAGCCG
TATGGCGGCT TCCTGGACAT TCTGCGGGGT GGGACAGAGC GGCTCTTCAC CAACCTCAAG
GACACCTCCT CCAAGGTCAT CCAGTCCGTC GCTAATTATG CAAAGGGTGA CCTGGACATA
TCTTACATCA CATCCAGAAT TGCAGTGATG TCATTCCCAG CAGAAGGTGT GGAGTCAGCG
CTCAAAAACA ACATCGAAGA TGTGCGGTTG TTCCTGGACT CCAAGCACCC AGGGCACTAT
GCCGTCTACA ACCTGTCCCC GAGGACCTAC CGGCCCTCCA GGTTCCACAA CCGGGTCTCC
GAGTGTGGCT GGGCAGCACG GCGGGCCCCA CACCTGCACA CCCTGTACAA CATCTGCAGG
AACATGCACG CCTGGCTGCG GCAGGACCAC AAGAACGTCT GCGTCGTGCA CTGCATGGAC
GGGAGAGCCG CGTCTGCTGT GGCCGTCTGC TCCTTCCTGT GCTTCTGCCG TCTCTTCAGC
ACCGCGGAGG CCGCCGTGTA CATGTTCAGC ATGAAGCGCT GCCCACCAGG CATCTGGCCA
TCCCACAAAA GGTACATCGA GTACATGTGT GACATGGTGG CGGAGGAGCC CATCACACCC
CACAGCAAGC CCATCCTGGT GAGGGCCGTG GTCATGACAC CCGTGCCGCT GTTCAGCAAG
CAGAGGAGCG GCTGCAGGCC CTTCTGCGAG GTCTACGTGG GGGACGAGCG TGTGGCCAGC
ACCTCCCAGG AGTACGACAA GATGCGGGAC TTTAAGATTG AAGATGGCAA AGCGGTGATT
CCCCTGGGCG TCACGGTGCA AGGAGACGTG CTCATCGTCA TCTATCACGC CCGGTCCACT
CTGGGCGGCC GGCTGCAGGC CAAGATGGCA TCCATGAAGA TGTTCCAGAT TCAGTTCCAC
ACGGGGTTTG TGCCTCGGAA CGCCACCACT GTGAAATTTG CCAAGTATGA CCTGGACGCG
TGTGACATTC AAGAAAAATA CCCGGATTTA TTTCAAGTGA ACCTGGAAGT GGAGGTGGAG
CCCAGGGACA GGCCGAGCCG GGAAGCCCCA CCATGGGAGA ACTCGAGCAT GAGGGGGCTG
AACCCCAAAA TCCTGTTTTC CAGCCGGGAG GAGCAGCAAG ACATTCTGTC TAAGTTTGGG
AAGCCGGAGC TTCCCCGGCA GCCTGGCTCC ACGGCTCAGT ATGATGCTGG GGCAGGGTCC
CCGGAAGCCG AACCCACAGA CTCTGACTCA CCGCCAAGCA GCAGCGCGGA CGCCAGTCGC
TTCCTGCACA CGCTGGACTG GCAGGAAGAG AAGGAGGCAG AGACTGGTGC AGAAAATGCC
TCTTCCAAGG AGAGCGAGTC TGCCCTGATG GAGGACAGAG ACGAGAGTGA GGTGTCAGAT
GAAGGGGGAT CCCCGATCTC CAGCGAGGGC CAGGAACCCA GGGCCGACCC AGAGCCCCCC
GGCCTGGCAG CAGGGCTGGT GCAGCAGGAC TTGGTTTTTG AGGTGGAGAC ACCGGCTGTG
CTGCCAGAGC CTGTGCCACA GGAAGACGGG GTCGACCTCC TGGGCCTGCA CTCCGAGGTG
GGCGCAGGGC CAGCTGTACC CCCGCAGGCC TGCAAGGCCC CCTCCAGCAA CACCGACCTG
CTCAGCTGCC TCCTTGGGCC CCCTGAGGCC GCCTCCCAGG GGCCCCCGGA GGATCTGCTC
AGCGAGGACC CGCTGCTCCT GGCAAGCCCG GCCCCTCCCC TGAGCGTGCA GAGCACCCCA
AGAGGAGGGC CCCCTGCCGC TGCTGACCCC TTTGGCCCGC TTCTGCCGTC TTCAGGCAAC
AACTCCCAGC CCTGCTCCAA TCCTGATCTC TTCGGCGAAT TTCTCAATTC GGACTCTGTG
ACCGTCCCAC CATCCTTCCC GTCTGCCCAC AGTGCTCCGC CCCCATCCTG CAGCGCCGAC
TTCCTGCACC TGGGGGATCT GCCAGGAGAG CCCAGCAAGA TGACAGCCTC GTCCAGCAAC
CCAGACCTGC TGGGAGGATG GGCTGCCTGG ACCGAGACTG CAGCATCGGC AGTGGCCCCC
ACGCCAGCCA CAGAAGGCCC CCTCTTCTCT CCTGGAGGTC AGCCGGCCCC TTGTGGCTCT
CAGGCCAGCT GGACCAAGTC TCAGAACCCG GACCCATTTG CTGACCTTGG CGACCTCAGC
TCCGGCCTCC AAGGCTCACC AGCTGGATTC CCTCCTGGGG GCTTCATTCC CAAAACGGCC
ACCACGCCCA AAGGCAGCAG CTCCTGGCAG ACAAGTCGGC CGCCAGCCCA GGGCGCCTCA
TGGCCCCCTC AGGCCAAGCC GCCCCCCAAA GCCTGCACAC AGCCAAGGCC TAACTATGCC
TCGAACTTCA GTGTGATCGG GGCGCGGGAG GAGCGGGGGG TCCGCGCACC CAGCTTTGCT
CAAAAGCCAA AAGTCTCTGA GAACGACTTT GAAGATCTGT TGTCCAATCA AGGCTTCTCC
TCCAGGTCTG ACAAGAAAGG GCCAAAGACC ATTGCAGAGA TGAGGAAGCA GGACCTGGCT
AAAGACACGG ACCCACTCAA GCTGAAGCTC CTGGACTGGA TTGAGGGCAA GGAGCGGAAC
ATCCGGGCCC TGCTGTCCAC GCTGCACACA GTGCTGTGGG ACGGGGAGAG CCGCTGGACG
CCCGTGGGCA TGGCCGACCT GGTGGCTCCG GAGCAAGTGA AGAAGCACTA TCGCCGCGCG
GTGCTGGCTG TGCACCCCGA CAAGGCTGCG GGGCAGCCGT ACGAGCAGCA CGCCAAGATG
ATCTTCATGG AGCTGAATGA CGCCTGGTCG GAGTTTGAGA ACCAGGGCTC CCGGCCCCTC
TTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu95042
Clone ID Related Accession (Same CDS sequence) XM_017007992.1
Accession Version XM_017007992.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3741bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product cyclin-G-associated kinase isoform X11
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGGCAG CTGGTGGAAT TTTTGAAGAA AATGGAATCT
CGAGGCCCCC TTTCGTGCGA CACGGTTCTG AAGATCTTCT ACCAGACGTG CCGCGCCGTG
CAGCACATGC ACCGGCAGAA GCCGCCCATC ATCCACAGGG ACCTCAAGGT TGAGAACTTG
TTGCTTAGTA ACCAAGGGAC CATTAAGCTG TGTGACTTTG GCAGTGCCAC GACCATCTCG
CACTACCCTG ACTACAGCTG GAGCGCCCAG AGGCGAGCCC TGGTGGAGGA AGAGGCCCTG
GGCTGCATCT TGTACCTGCT GTGCTTCCGG CAGCACCCTT TTGAGGATGG AGCGAAACTT
CGAATAGTCA ATGGGAAGTA CTCGATCCCC CCGCACGACA CGCAGTACAC GGTCTTCCAC
AGCCTCATCC GCGCCATGCT GCAGGTGAAC CCGGAGGAGC GGCTGTCCAT CGCCGAGGTG
GTGCACCAGC TGCAGGAGAT CGCGGCCGCC CGCAACGTGA ACCCCAAGTC TCCCATCACA
GAGCTCCTGG AGCAGAATGG AGGCTACGGG AGCGCCACAC TGTCCCGAGG GCCACCCCCT
CCCGTGGGCC CCGCTGGCAG TGGCTACAGT GGAGGCCTGG CGCTGGCGGA GTACGACCAG
CCGTATGGCG GCTTCCTGGA CATTCTGCGG GGTGGGACAG AGCGGCTCTT CACCAACCTC
AAGGACACCT CCTCCAAGGT CATCCAGTCC GTCGCTAATT ATGCAAAGGG TGACCTGGAC
ATATCTTACA TCACATCCAG AATTGCAGTG ATGTCATTCC CAGCAGAAGG TGTGGAGTCA
GCGCTCAAAA ACAACATCGA AGATGTGCGG TTGTTCCTGG ACTCCAAGCA CCCAGGGCAC
TATGCCGTCT ACAACCTGTC CCCGAGGACC TACCGGCCCT CCAGGTTCCA CAACCGGGTC
TCCGAGTGTG GCTGGGCAGC ACGGCGGGCC CCACACCTGC ACACCCTGTA CAACATCTGC
AGGAACATGC ACGCCTGGCT GCGGCAGGAC CACAAGAACG TCTGCGTCGT GCACTGCATG
GACGGGAGAG CCGCGTCTGC TGTGGCCGTC TGCTCCTTCC TGTGCTTCTG CCGTCTCTTC
AGCACCGCGG AGGCCGCCGT GTACATGTTC AGCATGAAGC GCTGCCCACC AGGCATCTGG
CCATCCCACA AAAGGTACAT CGAGTACATG TGTGACATGG TGGCGGAGGA GCCCATCACA
CCCCACAGCA AGCCCATCCT GGTGAGGGCC GTGGTCATGA CACCCGTGCC GCTGTTCAGC
AAGCAGAGGA GCGGCTGCAG GCCCTTCTGC GAGGTCTACG TGGGGGACGA GCGTGTGGCC
AGCACCTCCC AGGAGTACGA CAAGATGCGG GACTTTAAGA TTGAAGATGG CAAAGCGGTG
ATTCCCCTGG GCGTCACGGT GCAAGGAGAC GTGCTCATCG TCATCTATCA CGCCCGGTCC
ACTCTGGGCG GCCGGCTGCA GGCCAAGATG GCATCCATGA AGATGTTCCA GATTCAGTTC
CACACGGGGT TTGTGCCTCG GAACGCCACC ACTGTGAAAT TTGCCAAGTA TGACCTGGAC
GCGTGTGACA TTCAAGAAAA ATACCCGGAT TTATTTCAAG TGAACCTGGA AGTGGAGGTG
GAGCCCAGGG ACAGGCCGAG CCGGGAAGCC CCACCATGGG AGAACTCGAG CATGAGGGGG
CTGAACCCCA AAATCCTGTT TTCCAGCCGG GAGGAGCAGC AAGACATTCT GTCTAAGTTT
GGGAAGCCGG AGCTTCCCCG GCAGCCTGGC TCCACGGCTC AGTATGATGC TGGGGCAGGG
TCCCCGGAAG CCGAACCCAC AGACTCTGAC TCACCGCCAA GCAGCAGCGC GGACGCCAGT
CGCTTCCTGC ACACGCTGGA CTGGCAGGAA GAGAAGGAGG CAGAGACTGG TGCAGAAAAT
GCCTCTTCCA AGGAGAGCGA GTCTGCCCTG ATGGAGGACA GAGACGAGAG TGAGGTGTCA
GATGAAGGGG GATCCCCGAT CTCCAGCGAG GGCCAGGAAC CCAGGGCCGA CCCAGAGCCC
CCCGGCCTGG CAGCAGGGCT GGTGCAGCAG GACTTGGTTT TTGAGGTGGA GACACCGGCT
GTGCTGCCAG AGCCTGTGCC ACAGGAAGAC GGGGTCGACC TCCTGGGCCT GCACTCCGAG
GTGGGCGCAG GGCCAGCTGT ACCCCCGCAG GCCTGCAAGG CCCCCTCCAG CAACACCGAC
CTGCTCAGCT GCCTCCTTGG GCCCCCTGAG GCCGCCTCCC AGGGGCCCCC GGAGGATCTG
CTCAGCGAGG ACCCGCTGCT CCTGGCAAGC CCGGCCCCTC CCCTGAGCGT GCAGAGCACC
CCAAGAGGAG GGCCCCCTGC CGCTGCTGAC CCCTTTGGCC CGCTTCTGCC GTCTTCAGGC
AACAACTCCC AGCCCTGCTC CAATCCTGAT CTCTTCGGCG AATTTCTCAA TTCGGACTCT
GTGACCGTCC CACCATCCTT CCCGTCTGCC CACAGTGCTC CGCCCCCATC CTGCAGCGCC
GACTTCCTGC ACCTGGGGGA TCTGCCAGGA GAGCCCAGCA AGATGACAGC CTCGTCCAGC
AACCCAGACC TGCTGGGAGG ATGGGCTGCC TGGACCGAGA CTGCAGCATC GGCAGTGGCC
CCCACGCCAG CCACAGAAGG CCCCCTCTTC TCTCCTGGAG GTCAGCCGGC CCCTTGTGGC
TCTCAGGCCA GCTGGACCAA GTCTCAGAAC CCGGACCCAT TTGCTGACCT TGGCGACCTC
AGCTCCGGCC TCCAAGACCC CCAAGCCCAG AGCACAGTGA GCCCAAGGGG ACAGCGTGTC
TGCACCTGTT CCAGGCGACT GCCAACTGGC AAGCTAAAAC CGGGAGTTGC TGACACTGGC
ACTGCTGCCA GCCCCCACCG GCATTGTGGC TCACCAGCTG GATTCCCTCC TGGGGGCTTC
ATTCCCAAAA CGGCCACCAC GCCCAAAGGC AGCAGCTCCT GGCAGACAAG TCGGCCGCCA
GCCCAGGGCG CCTCATGGCC CCCTCAGGCC AAGCCGCCCC CCAAAGCCTG CACACAGCCA
AGGCCTAACT ATGCCTCGAA CTTCAGTGTG ATCGGGGCGC GGGAGGAGCG GGGGGTCCGC
GCACCCAGCT TTGCTCAAAA GCCAAAAGTC TCTGAGAACG ACTTTGAAGA TCTGTTGTCC
AATCAAGGCT TCTCCTCCAG GTCTGACAAG AAAGGGCCAA AGACCATTGC AGAGATGAGG
AAGCAGGACC TGGCTAAAGA CACGGACCCA CTCAAGCTGA AGCTCCTGGA CTGGATTGAG
GGCAAGGAGC GGAACATCCG GGCCCTGCTG TCCACGCTGC ACACAGTGCT GTGGGACGGG
GAGAGCCGCT GGACGCCCGT GGGCATGGCC GACCTGGTGG CTCCGGAGCA AGTGAAGAAG
CACTATCGCC GCGCGGTGCT GGCTGTGCAC CCCGACAAGG CTGCGGGGCA GCCGTACGAG
CAGCACGCCA AGATGATCTT CATGGAGCTG AATGACGCCT GGTCGGAGTT TGAGAACCAG
GGCTCCCGGC CCCTCTTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016863481.1
CDS128..3868
Translation

Target ORF information:

RefSeq Version XM_017007992.1
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin G associated kinase (GAK), transcript variant X11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017007992.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGGCAG CTGGTGGAAT TTTTGAAGAA AATGGAATCT
CGAGGCCCCC TTTCGTGCGA CACGGTTCTG AAGATCTTCT ACCAGACGTG CCGCGCCGTG
CAGCACATGC ACCGGCAGAA GCCGCCCATC ATCCACAGGG ACCTCAAGGT TGAGAACTTG
TTGCTTAGTA ACCAAGGGAC CATTAAGCTG TGTGACTTTG GCAGTGCCAC GACCATCTCG
CACTACCCTG ACTACAGCTG GAGCGCCCAG AGGCGAGCCC TGGTGGAGGA AGAGGCCCTG
GGCTGCATCT TGTACCTGCT GTGCTTCCGG CAGCACCCTT TTGAGGATGG AGCGAAACTT
CGAATAGTCA ATGGGAAGTA CTCGATCCCC CCGCACGACA CGCAGTACAC GGTCTTCCAC
AGCCTCATCC GCGCCATGCT GCAGGTGAAC CCGGAGGAGC GGCTGTCCAT CGCCGAGGTG
GTGCACCAGC TGCAGGAGAT CGCGGCCGCC CGCAACGTGA ACCCCAAGTC TCCCATCACA
GAGCTCCTGG AGCAGAATGG AGGCTACGGG AGCGCCACAC TGTCCCGAGG GCCACCCCCT
CCCGTGGGCC CCGCTGGCAG TGGCTACAGT GGAGGCCTGG CGCTGGCGGA GTACGACCAG
CCGTATGGCG GCTTCCTGGA CATTCTGCGG GGTGGGACAG AGCGGCTCTT CACCAACCTC
AAGGACACCT CCTCCAAGGT CATCCAGTCC GTCGCTAATT ATGCAAAGGG TGACCTGGAC
ATATCTTACA TCACATCCAG AATTGCAGTG ATGTCATTCC CAGCAGAAGG TGTGGAGTCA
GCGCTCAAAA ACAACATCGA AGATGTGCGG TTGTTCCTGG ACTCCAAGCA CCCAGGGCAC
TATGCCGTCT ACAACCTGTC CCCGAGGACC TACCGGCCCT CCAGGTTCCA CAACCGGGTC
TCCGAGTGTG GCTGGGCAGC ACGGCGGGCC CCACACCTGC ACACCCTGTA CAACATCTGC
AGGAACATGC ACGCCTGGCT GCGGCAGGAC CACAAGAACG TCTGCGTCGT GCACTGCATG
GACGGGAGAG CCGCGTCTGC TGTGGCCGTC TGCTCCTTCC TGTGCTTCTG CCGTCTCTTC
AGCACCGCGG AGGCCGCCGT GTACATGTTC AGCATGAAGC GCTGCCCACC AGGCATCTGG
CCATCCCACA AAAGGTACAT CGAGTACATG TGTGACATGG TGGCGGAGGA GCCCATCACA
CCCCACAGCA AGCCCATCCT GGTGAGGGCC GTGGTCATGA CACCCGTGCC GCTGTTCAGC
AAGCAGAGGA GCGGCTGCAG GCCCTTCTGC GAGGTCTACG TGGGGGACGA GCGTGTGGCC
AGCACCTCCC AGGAGTACGA CAAGATGCGG GACTTTAAGA TTGAAGATGG CAAAGCGGTG
ATTCCCCTGG GCGTCACGGT GCAAGGAGAC GTGCTCATCG TCATCTATCA CGCCCGGTCC
ACTCTGGGCG GCCGGCTGCA GGCCAAGATG GCATCCATGA AGATGTTCCA GATTCAGTTC
CACACGGGGT TTGTGCCTCG GAACGCCACC ACTGTGAAAT TTGCCAAGTA TGACCTGGAC
GCGTGTGACA TTCAAGAAAA ATACCCGGAT TTATTTCAAG TGAACCTGGA AGTGGAGGTG
GAGCCCAGGG ACAGGCCGAG CCGGGAAGCC CCACCATGGG AGAACTCGAG CATGAGGGGG
CTGAACCCCA AAATCCTGTT TTCCAGCCGG GAGGAGCAGC AAGACATTCT GTCTAAGTTT
GGGAAGCCGG AGCTTCCCCG GCAGCCTGGC TCCACGGCTC AGTATGATGC TGGGGCAGGG
TCCCCGGAAG CCGAACCCAC AGACTCTGAC TCACCGCCAA GCAGCAGCGC GGACGCCAGT
CGCTTCCTGC ACACGCTGGA CTGGCAGGAA GAGAAGGAGG CAGAGACTGG TGCAGAAAAT
GCCTCTTCCA AGGAGAGCGA GTCTGCCCTG ATGGAGGACA GAGACGAGAG TGAGGTGTCA
GATGAAGGGG GATCCCCGAT CTCCAGCGAG GGCCAGGAAC CCAGGGCCGA CCCAGAGCCC
CCCGGCCTGG CAGCAGGGCT GGTGCAGCAG GACTTGGTTT TTGAGGTGGA GACACCGGCT
GTGCTGCCAG AGCCTGTGCC ACAGGAAGAC GGGGTCGACC TCCTGGGCCT GCACTCCGAG
GTGGGCGCAG GGCCAGCTGT ACCCCCGCAG GCCTGCAAGG CCCCCTCCAG CAACACCGAC
CTGCTCAGCT GCCTCCTTGG GCCCCCTGAG GCCGCCTCCC AGGGGCCCCC GGAGGATCTG
CTCAGCGAGG ACCCGCTGCT CCTGGCAAGC CCGGCCCCTC CCCTGAGCGT GCAGAGCACC
CCAAGAGGAG GGCCCCCTGC CGCTGCTGAC CCCTTTGGCC CGCTTCTGCC GTCTTCAGGC
AACAACTCCC AGCCCTGCTC CAATCCTGAT CTCTTCGGCG AATTTCTCAA TTCGGACTCT
GTGACCGTCC CACCATCCTT CCCGTCTGCC CACAGTGCTC CGCCCCCATC CTGCAGCGCC
GACTTCCTGC ACCTGGGGGA TCTGCCAGGA GAGCCCAGCA AGATGACAGC CTCGTCCAGC
AACCCAGACC TGCTGGGAGG ATGGGCTGCC TGGACCGAGA CTGCAGCATC GGCAGTGGCC
CCCACGCCAG CCACAGAAGG CCCCCTCTTC TCTCCTGGAG GTCAGCCGGC CCCTTGTGGC
TCTCAGGCCA GCTGGACCAA GTCTCAGAAC CCGGACCCAT TTGCTGACCT TGGCGACCTC
AGCTCCGGCC TCCAAGACCC CCAAGCCCAG AGCACAGTGA GCCCAAGGGG ACAGCGTGTC
TGCACCTGTT CCAGGCGACT GCCAACTGGC AAGCTAAAAC CGGGAGTTGC TGACACTGGC
ACTGCTGCCA GCCCCCACCG GCATTGTGGC TCACCAGCTG GATTCCCTCC TGGGGGCTTC
ATTCCCAAAA CGGCCACCAC GCCCAAAGGC AGCAGCTCCT GGCAGACAAG TCGGCCGCCA
GCCCAGGGCG CCTCATGGCC CCCTCAGGCC AAGCCGCCCC CCAAAGCCTG CACACAGCCA
AGGCCTAACT ATGCCTCGAA CTTCAGTGTG ATCGGGGCGC GGGAGGAGCG GGGGGTCCGC
GCACCCAGCT TTGCTCAAAA GCCAAAAGTC TCTGAGAACG ACTTTGAAGA TCTGTTGTCC
AATCAAGGCT TCTCCTCCAG GTCTGACAAG AAAGGGCCAA AGACCATTGC AGAGATGAGG
AAGCAGGACC TGGCTAAAGA CACGGACCCA CTCAAGCTGA AGCTCCTGGA CTGGATTGAG
GGCAAGGAGC GGAACATCCG GGCCCTGCTG TCCACGCTGC ACACAGTGCT GTGGGACGGG
GAGAGCCGCT GGACGCCCGT GGGCATGGCC GACCTGGTGG CTCCGGAGCA AGTGAAGAAG
CACTATCGCC GCGCGGTGCT GGCTGTGCAC CCCGACAAGG CTGCGGGGCA GCCGTACGAG
CAGCACGCCA AGATGATCTT CATGGAGCTG AATGACGCCT GGTCGGAGTT TGAGAACCAG
GGCTCCCGGC CCCTCTTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu95043
Clone ID Related Accession (Same CDS sequence) XM_017007994.1
Accession Version XM_017007994.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3594bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product cyclin-G-associated kinase isoform X14
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATGTG
GGGAGTGGCA GAGAGTATGC ATTAAAGAGG CTATTATCCA ATGAAGAGGA AAAGAACAGA
GCCATCATTC AAGAAGTTTG CTTCATGAAA AAGCTTTCCG GCCACCCGAA CATTGTCCAG
TTTTGTTCTG CAGCGTCTAT AGGAAAAGAG GAGTCAGACA CGGGGCAGGC TGAGTTCCTC
TTGCTCACAG AGCTCTGTAA AGGGCAGCTG GTGGAATTTT TGAAGAAAAT GGAATCTCGA
GGCCCCCTTT CGTGCGACAC GGTTCTGAAG ATCTTCTACC AGACGTGCCG CGCCGTGCAG
CACATGCACC GGCAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA GAACTTGTTG
CTTAGTAACC AAGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACGAC CATCTCGCAC
TACCCTGACT ACAGCTGGAG CGCCCAGAGG CGAGCCCTGG TGGAGGAAGA GATCACGAGG
AATACAACAC CAATGTATAG AACACCAGAA ATCATAGACT TGTATTCCAA CTTCCCGATC
GGCGAGAAGC AGGATATCTG GGCCCTGGGC TGCATCTTGT ACCTGCTGTG CTTCCGGCAG
CACCCTTTTG AGGATGGAGC GAAACTTCGA ATAGTCAATG GGAAGTACTC GATCCCCCCG
CACGACACGC AGTACACGGT CTTCCACAGC CTCATCCGCG CCATGCTGCA GGTGAACCCG
GAGGAGCGGC TGTCCATCGC CGAGGTGGTG CACCAGCTGC AGGAGATCGC GGCCGCCCGC
AACGTGAACC CCAAGTCTCC CATCACAGAG CTCCTGGAGC AGAATGGAGG CTACGGGAGC
GCCACACTGT CCCGAGGGCC ACCCCCTCCC GTGGGCCCCG CTGGCAGTGG CTACAGTGGA
GGCCTGGCGC TGGCGGAGTA CGACCAGCCG TATGGCGGCT TCCTGGACAT TCTGCGGGGT
GGGACAGAGC GGCTCTTCAC CAACCTCAAG GACACCTCCT CCAAGGTCAT CCAGTCCGTC
GCTAATTATG CAAAGGGTGA CCTGGACATA TCTTACATCA CATCCAGAAT TGCAGTGATG
TCATTCCCAG CAGAAGGTGT GGAGTCAGCG CTCAAAAACA ACATCGAAGA TGTGCGGTTG
TTCCTGGACT CCAAGCACCC AGGGCACTAT GCCGTCTACA ACCTGTCCCC GAGGACCTAC
CGGCCCTCCA GGTTCCACAA CCGGGTCTCC GAGTGTGGCT GGGCAGCACG GCGGGCCCCA
CACCTGCACA CCCTGTACAA CATCTGCAGG AACATGCACG CCTGGCTGCG GCAGGACCAC
AAGAACGTCT GCGTCGTGCA CTGCATGGAC GGGAGAGCCG CGTCTGCTGT GGCCGTCTGC
TCCTTCCTGT GCTTCTGCCG TCTCTTCAGC ACCGCGGAGG CCGCCGTGTA CATGTTCAGC
ATGAAGCGCT GCCCACCAGG CATCTGGCCA TCCCACAAAA GGTACATCGA GTACATGTGT
GACATGGTGG CGGAGGAGCC CATCACACCC CACAGCAAGC CCATCCTGGT GAGGGCCGTG
GTCATGACAC CCGTGCCGCT GTTCAGCAAG CAGAGGAGCG GCTGCAGGCC CTTCTGCGAG
GTCTACGTGG GGGACGAGCG TGTGGCCAGC ACCTCCCAGG AGTACGACAA GATGCGGGAC
TTTAAGATTG AAGATGGCAA AGCGGTGATT CCCCTGGGCG TCACGGTGCA AGGAGACGTG
CTCATCGTCA TCTATCACGC CCGGTCCACT CTGGGCGGCC GGCTGCAGGC CAAGATGGCA
TCCATGAAGA TGTTCCAGAT TCAGTTCCAC ACGGGGTTTG TGCCTCGGAA CGCCACCACT
GTGAAATTTG CCAAGTATGA CCTGGACGCG TGTGACATTC AAGAAAAATA CCCGGATTTA
TTTCAAGTGA ACCTGGAAGT GGAGGTGGAG CCCAGGGACA GGCCGAGCCG GGAAGCCCCA
CCATGGGAGA ACTCGAGCAT GAGGGGGCTG AACCCCAAAA TCCTGTTTTC CAGCCGGGAG
GAGCAGCAAG ACATTCTGTC TAAGTTTGGG AAGCCGGAGC TTCCCCGGCA GCCTGGCTCC
ACGGCTCAGT ATGATGCTGG GGCAGGGTCC CCGGAAGCCG AACCCACAGA CTCTGACTCA
CCGCCAAGCA GCAGCGCGGA CGCCAGTCGC TTCCTGCACA CGCTGGACTG GCAGGAAGAG
AAGGAGGCAG AGACTGGTGC AGAAAATGCC TCTTCCAAGG AGAGCGAGTC TGCCCTGATG
GAGGACAGAG ACGAGAGTGA GGTGTCAGAT GAAGGGGGAT CCCCGATCTC CAGCGAGGGC
CAGGAACCCA GGGCCGACCC AGAGCCCCCC GGCCTGGCAG CAGGGCTGGT GCAGCAGGAC
TTGGTTTTTG AGGTGGAGAC ACCGGCTGTG CTGCCAGAGC CTGTGCCACA GGAAGACGGG
GTCGACCTCC TGGGCCTGCA CTCCGAGGTG GGCGCAGGGC CAGCTGTACC CCCGCAGGCC
TGCAAGGCCC CCTCCAGCAA CACCGACCTG CTCAGCTGCC TCCTTGGGCC CCCTGAGGCC
GCCTCCCAGG GGCCCCCGGA GGATCTGCTC AGCGAGGACC CGCTGCTCCT GGCAAGCCCG
GCCCCTCCCC TGAGCGTGCA GAGCACCCCA AGAGGAGGGC CCCCTGCCGC TGCTGACCCC
TTTGGCCCGC TTCTGCCGTC TTCAGGCAAC AACTCCCAGC CCTGCTCCAA TCCTGATCTC
TTCGGCGAAT TTCTCAATTC GGACTCTGTG ACCGTCCCAC CATCCTTCCC GTCTGCCCAC
AGTGCTCCGC CCCCATCCTG CAGCGCCGAC TTCCTGCACC TGGGGGATCT GCCAGGAGAG
CCCAGCAAGA TGACAGCCTC GTCCAGCAAC CCAGACCTGC TGGGAGGATG GGCTGCCTGG
ACCGAGACTG CAGCATCGGC AGTGGCCCCC ACGCCAGCCA CAGAAGGCTC ACCAGCTGGA
TTCCCTCCTG GGGGCTTCAT TCCCAAAACG GCCACCACGC CCAAAGGCAG CAGCTCCTGG
CAGACAAGTC GGCCGCCAGC CCAGGGCGCC TCATGGCCCC CTCAGGCCAA GCCGCCCCCC
AAAGCCTGCA CACAGCCAAG GCCTAACTAT GCCTCGAACT TCAGTGTGAT CGGGGCGCGG
GAGGAGCGGG GGGTCCGCGC ACCCAGCTTT GGTGAGTCCC CCACTCTCTG TTGCTGTGGA
GTTTGCAGAG ACCTCCCTGA ATTGGCTAAA GGTAGAACCT ATGTTGTTTT CTTCATCTCA
GAGCCCAGTC CTGGGACAGG CACGGCCAAG CCACTCAGAG AACGACGCGG TTTCCGATGT
GTGTTCACTG GTCTCTCTCT GGGTCAGGCC TCTGGGTCCC TCTTGTTCCA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016863483.1
CDS144..3737
Translation

Target ORF information:

RefSeq Version XM_017007994.1
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin G associated kinase (GAK), transcript variant X15, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017007994.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATGTG
GGGAGTGGCA GAGAGTATGC ATTAAAGAGG CTATTATCCA ATGAAGAGGA AAAGAACAGA
GCCATCATTC AAGAAGTTTG CTTCATGAAA AAGCTTTCCG GCCACCCGAA CATTGTCCAG
TTTTGTTCTG CAGCGTCTAT AGGAAAAGAG GAGTCAGACA CGGGGCAGGC TGAGTTCCTC
TTGCTCACAG AGCTCTGTAA AGGGCAGCTG GTGGAATTTT TGAAGAAAAT GGAATCTCGA
GGCCCCCTTT CGTGCGACAC GGTTCTGAAG ATCTTCTACC AGACGTGCCG CGCCGTGCAG
CACATGCACC GGCAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA GAACTTGTTG
CTTAGTAACC AAGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACGAC CATCTCGCAC
TACCCTGACT ACAGCTGGAG CGCCCAGAGG CGAGCCCTGG TGGAGGAAGA GATCACGAGG
AATACAACAC CAATGTATAG AACACCAGAA ATCATAGACT TGTATTCCAA CTTCCCGATC
GGCGAGAAGC AGGATATCTG GGCCCTGGGC TGCATCTTGT ACCTGCTGTG CTTCCGGCAG
CACCCTTTTG AGGATGGAGC GAAACTTCGA ATAGTCAATG GGAAGTACTC GATCCCCCCG
CACGACACGC AGTACACGGT CTTCCACAGC CTCATCCGCG CCATGCTGCA GGTGAACCCG
GAGGAGCGGC TGTCCATCGC CGAGGTGGTG CACCAGCTGC AGGAGATCGC GGCCGCCCGC
AACGTGAACC CCAAGTCTCC CATCACAGAG CTCCTGGAGC AGAATGGAGG CTACGGGAGC
GCCACACTGT CCCGAGGGCC ACCCCCTCCC GTGGGCCCCG CTGGCAGTGG CTACAGTGGA
GGCCTGGCGC TGGCGGAGTA CGACCAGCCG TATGGCGGCT TCCTGGACAT TCTGCGGGGT
GGGACAGAGC GGCTCTTCAC CAACCTCAAG GACACCTCCT CCAAGGTCAT CCAGTCCGTC
GCTAATTATG CAAAGGGTGA CCTGGACATA TCTTACATCA CATCCAGAAT TGCAGTGATG
TCATTCCCAG CAGAAGGTGT GGAGTCAGCG CTCAAAAACA ACATCGAAGA TGTGCGGTTG
TTCCTGGACT CCAAGCACCC AGGGCACTAT GCCGTCTACA ACCTGTCCCC GAGGACCTAC
CGGCCCTCCA GGTTCCACAA CCGGGTCTCC GAGTGTGGCT GGGCAGCACG GCGGGCCCCA
CACCTGCACA CCCTGTACAA CATCTGCAGG AACATGCACG CCTGGCTGCG GCAGGACCAC
AAGAACGTCT GCGTCGTGCA CTGCATGGAC GGGAGAGCCG CGTCTGCTGT GGCCGTCTGC
TCCTTCCTGT GCTTCTGCCG TCTCTTCAGC ACCGCGGAGG CCGCCGTGTA CATGTTCAGC
ATGAAGCGCT GCCCACCAGG CATCTGGCCA TCCCACAAAA GGTACATCGA GTACATGTGT
GACATGGTGG CGGAGGAGCC CATCACACCC CACAGCAAGC CCATCCTGGT GAGGGCCGTG
GTCATGACAC CCGTGCCGCT GTTCAGCAAG CAGAGGAGCG GCTGCAGGCC CTTCTGCGAG
GTCTACGTGG GGGACGAGCG TGTGGCCAGC ACCTCCCAGG AGTACGACAA GATGCGGGAC
TTTAAGATTG AAGATGGCAA AGCGGTGATT CCCCTGGGCG TCACGGTGCA AGGAGACGTG
CTCATCGTCA TCTATCACGC CCGGTCCACT CTGGGCGGCC GGCTGCAGGC CAAGATGGCA
TCCATGAAGA TGTTCCAGAT TCAGTTCCAC ACGGGGTTTG TGCCTCGGAA CGCCACCACT
GTGAAATTTG CCAAGTATGA CCTGGACGCG TGTGACATTC AAGAAAAATA CCCGGATTTA
TTTCAAGTGA ACCTGGAAGT GGAGGTGGAG CCCAGGGACA GGCCGAGCCG GGAAGCCCCA
CCATGGGAGA ACTCGAGCAT GAGGGGGCTG AACCCCAAAA TCCTGTTTTC CAGCCGGGAG
GAGCAGCAAG ACATTCTGTC TAAGTTTGGG AAGCCGGAGC TTCCCCGGCA GCCTGGCTCC
ACGGCTCAGT ATGATGCTGG GGCAGGGTCC CCGGAAGCCG AACCCACAGA CTCTGACTCA
CCGCCAAGCA GCAGCGCGGA CGCCAGTCGC TTCCTGCACA CGCTGGACTG GCAGGAAGAG
AAGGAGGCAG AGACTGGTGC AGAAAATGCC TCTTCCAAGG AGAGCGAGTC TGCCCTGATG
GAGGACAGAG ACGAGAGTGA GGTGTCAGAT GAAGGGGGAT CCCCGATCTC CAGCGAGGGC
CAGGAACCCA GGGCCGACCC AGAGCCCCCC GGCCTGGCAG CAGGGCTGGT GCAGCAGGAC
TTGGTTTTTG AGGTGGAGAC ACCGGCTGTG CTGCCAGAGC CTGTGCCACA GGAAGACGGG
GTCGACCTCC TGGGCCTGCA CTCCGAGGTG GGCGCAGGGC CAGCTGTACC CCCGCAGGCC
TGCAAGGCCC CCTCCAGCAA CACCGACCTG CTCAGCTGCC TCCTTGGGCC CCCTGAGGCC
GCCTCCCAGG GGCCCCCGGA GGATCTGCTC AGCGAGGACC CGCTGCTCCT GGCAAGCCCG
GCCCCTCCCC TGAGCGTGCA GAGCACCCCA AGAGGAGGGC CCCCTGCCGC TGCTGACCCC
TTTGGCCCGC TTCTGCCGTC TTCAGGCAAC AACTCCCAGC CCTGCTCCAA TCCTGATCTC
TTCGGCGAAT TTCTCAATTC GGACTCTGTG ACCGTCCCAC CATCCTTCCC GTCTGCCCAC
AGTGCTCCGC CCCCATCCTG CAGCGCCGAC TTCCTGCACC TGGGGGATCT GCCAGGAGAG
CCCAGCAAGA TGACAGCCTC GTCCAGCAAC CCAGACCTGC TGGGAGGATG GGCTGCCTGG
ACCGAGACTG CAGCATCGGC AGTGGCCCCC ACGCCAGCCA CAGAAGGCTC ACCAGCTGGA
TTCCCTCCTG GGGGCTTCAT TCCCAAAACG GCCACCACGC CCAAAGGCAG CAGCTCCTGG
CAGACAAGTC GGCCGCCAGC CCAGGGCGCC TCATGGCCCC CTCAGGCCAA GCCGCCCCCC
AAAGCCTGCA CACAGCCAAG GCCTAACTAT GCCTCGAACT TCAGTGTGAT CGGGGCGCGG
GAGGAGCGGG GGGTCCGCGC ACCCAGCTTT GGTGAGTCCC CCACTCTCTG TTGCTGTGGA
GTTTGCAGAG ACCTCCCTGA ATTGGCTAAA GGTAGAACCT ATGTTGTTTT CTTCATCTCA
GAGCCCAGTC CTGGGACAGG CACGGCCAAG CCACTCAGAG AACGACGCGG TTTCCGATGT
GTGTTCACTG GTCTCTCTCT GGGTCAGGCC TCTGGGTCCC TCTTGTTCCA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu95044
Clone ID Related Accession (Same CDS sequence) XM_017007995.1
Accession Version XM_017007995.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3396bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product cyclin-G-associated kinase isoform X15
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGTATAGAA CACCAGAAAT CATAGACTTG TATTCCAACT TCCCGATCGG CGAGAAGCAG 
GATATCTGGG CCCTGGGCTG CATCTTGTAC CTGCTGTGCT TCCGGCAGCA CCCTTTTGAG
GATGGAGCGA AACTTCGAAT AGTCAATGGG AAGTACTCGA TCCCCCCGCA CGACACGCAG
TACACGGTCT TCCACAGCCT CATCCGCGCC ATGCTGCAGG TGAACCCGGA GGAGCGGCTG
TCCATCGCCG AGGTGGTGCA CCAGCTGCAG GAGATCGCGG CCGCCCGCAA CGTGAACCCC
AAGTCTCCCA TCACAGAGCT CCTGGAGCAG AATGGAGGCT ACGGGAGCGC CACACTGTCC
CGAGGGCCAC CCCCTCCCGT GGGCCCCGCT GGCAGTGGCT ACAGTGGAGG CCTGGCGCTG
GCGGAGTACG ACCAGCCGTA TGGCGGCTTC CTGGACATTC TGCGGGGTGG GACAGAGCGG
CTCTTCACCA ACCTCAAGGA CACCTCCTCC AAGGTCATCC AGTCCGTCGC TAATTATGCA
AAGGGTGACC TGGACATATC TTACATCACA TCCAGAATTG CAGTGATGTC ATTCCCAGCA
GAAGGTGTGG AGTCAGCGCT CAAAAACAAC ATCGAAGATG TGCGGTTGTT CCTGGACTCC
AAGCACCCAG GGCACTATGC CGTCTACAAC CTGTCCCCGA GGACCTACCG GCCCTCCAGG
TTCCACAACC GGGTCTCCGA GTGTGGCTGG GCAGCACGGC GGGCCCCACA CCTGCACACC
CTGTACAACA TCTGCAGGAA CATGCACGCC TGGCTGCGGC AGGACCACAA GAACGTCTGC
GTCGTGCACT GCATGGACGG GAGAGCCGCG TCTGCTGTGG CCGTCTGCTC CTTCCTGTGC
TTCTGCCGTC TCTTCAGCAC CGCGGAGGCC GCCGTGTACA TGTTCAGCAT GAAGCGCTGC
CCACCAGGCA TCTGGCCATC CCACAAAAGG TACATCGAGT ACATGTGTGA CATGGTGGCG
GAGGAGCCCA TCACACCCCA CAGCAAGCCC ATCCTGGTGA GGGCCGTGGT CATGACACCC
GTGCCGCTGT TCAGCAAGCA GAGGAGCGGC TGCAGGCCCT TCTGCGAGGT CTACGTGGGG
GACGAGCGTG TGGCCAGCAC CTCCCAGGAG TACGACAAGA TGCGGGACTT TAAGATTGAA
GATGGCAAAG CGGTGATTCC CCTGGGCGTC ACGGTGCAAG GAGACGTGCT CATCGTCATC
TATCACGCCC GGTCCACTCT GGGCGGCCGG CTGCAGGCCA AGATGGCATC CATGAAGATG
TTCCAGATTC AGTTCCACAC GGGGTTTGTG CCTCGGAACG CCACCACTGT GAAATTTGCC
AAGTATGACC TGGACGCGTG TGACATTCAA GAAAAATACC CGGATTTATT TCAAGTGAAC
CTGGAAGTGG AGGTGGAGCC CAGGGACAGG CCGAGCCGGG AAGCCCCACC ATGGGAGAAC
TCGAGCATGA GGGGGCTGAA CCCCAAAATC CTGTTTTCCA GCCGGGAGGA GCAGCAAGAC
ATTCTGTCTA AGTTTGGGAA GCCGGAGCTT CCCCGGCAGC CTGGCTCCAC GGCTCAGTAT
GATGCTGGGG CAGGGTCCCC GGAAGCCGAA CCCACAGACT CTGACTCACC GCCAAGCAGC
AGCGCGGACG CCAGTCGCTT CCTGCACACG CTGGACTGGC AGGAAGAGAA GGAGGCAGAG
ACTGGTGCAG AAAATGCCTC TTCCAAGGAG AGCGAGTCTG CCCTGATGGA GGACAGAGAC
GAGAGTGAGG TGTCAGATGA AGGGGGATCC CCGATCTCCA GCGAGGGCCA GGAACCCAGG
GCCGACCCAG AGCCCCCCGG CCTGGCAGCA GGGCTGGTGC AGCAGGACTT GGTTTTTGAG
GTGGAGACAC CGGCTGTGCT GCCAGAGCCT GTGCCACAGG AAGACGGGGT CGACCTCCTG
GGCCTGCACT CCGAGGTGGG CGCAGGGCCA GCTGTACCCC CGCAGGCCTG CAAGGCCCCC
TCCAGCAACA CCGACCTGCT CAGCTGCCTC CTTGGGCCCC CTGAGGCCGC CTCCCAGGGG
CCCCCGGAGG ATCTGCTCAG CGAGGACCCG CTGCTCCTGG CAAGCCCGGC CCCTCCCCTG
AGCGTGCAGA GCACCCCAAG AGGAGGGCCC CCTGCCGCTG CTGACCCCTT TGGCCCGCTT
CTGCCGTCTT CAGGCAACAA CTCCCAGCCC TGCTCCAATC CTGATCTCTT CGGCGAATTT
CTCAATTCGG ACTCTGTGAC CGTCCCACCA TCCTTCCCGT CTGCCCACAG TGCTCCGCCC
CCATCCTGCA GCGCCGACTT CCTGCACCTG GGGGATCTGC CAGGAGAGCC CAGCAAGATG
ACAGCCTCGT CCAGCAACCC AGACCTGCTG GGAGGATGGG CTGCCTGGAC CGAGACTGCA
GCATCGGCAG TGGCCCCCAC GCCAGCCACA GAAGGCCCCC TCTTCTCTCC TGGAGGTCAG
CCGGCCCCTT GTGGCTCTCA GGCCAGCTGG ACCAAGTCTC AGAACCCGGA CCCATTTGCT
GACCTTGGCG ACCTCAGCTC CGGCCTCCAA GACCCCCAAG CCCAGAGCAC AGTGAGCCCA
AGGGGACAGC GTGTCTGCAC CTGTTCCAGG CGACTGCCAA CTGGCAAGCT AAAACCGGGA
GTTGCTGACA CTGGCACTGC TGCCAGCCCC CACCGGCATT GTGGCTCACC AGCTGGATTC
CCTCCTGGGG GCTTCATTCC CAAAACGGCC ACCACGCCCA AAGGCAGCAG CTCCTGGCAG
ACAAGTCGGC CGCCAGCCCA GGGCGCCTCA TGGCCCCCTC AGGCCAAGCC GCCCCCCAAA
GCCTGCACAC AGCCAAGGCC TAACTATGCC TCGAACTTCA GTGTGATCGG GGCGCGGGAG
GAGCGGGGGG TCCGCGCACC CAGCTTTGCT CAAAAGCCAA AAGTCTCTGA GAACGACTTT
GAAGATCTGT TGTCCAATCA AGGCTTCTCC TCCAGGTCTG ACAAGAAAGG GCCAAAGACC
ATTGCAGAGA TGAGGAAGCA GGACCTGGCT AAAGACACGG ACCCACTCAA GCTGAAGCTC
CTGGACTGGA TTGAGGGCAA GGAGCGGAAC ATCCGGGCCC TGCTGTCCAC GCTGCACACA
GTGCTGTGGG ACGGGGAGAG CCGCTGGACG CCCGTGGGCA TGGCCGACCT GGTGGCTCCG
GAGCAAGTGA AGAAGCACTA TCGCCGCGCG GTGCTGGCTG TGCACCCCGA CAAGGCTGCG
GGGCAGCCGT ACGAGCAGCA CGCCAAGATG ATCTTCATGG AGCTGAATGA CGCCTGGTCG
GAGTTTGAGA ACCAGGGCTC CCGGCCCCTC TTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016863484.1
CDS635..4030
Translation

Target ORF information:

RefSeq Version XM_017007995.1
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin G associated kinase (GAK), transcript variant X16, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017007995.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGTATAGAA CACCAGAAAT CATAGACTTG TATTCCAACT TCCCGATCGG CGAGAAGCAG 
GATATCTGGG CCCTGGGCTG CATCTTGTAC CTGCTGTGCT TCCGGCAGCA CCCTTTTGAG
GATGGAGCGA AACTTCGAAT AGTCAATGGG AAGTACTCGA TCCCCCCGCA CGACACGCAG
TACACGGTCT TCCACAGCCT CATCCGCGCC ATGCTGCAGG TGAACCCGGA GGAGCGGCTG
TCCATCGCCG AGGTGGTGCA CCAGCTGCAG GAGATCGCGG CCGCCCGCAA CGTGAACCCC
AAGTCTCCCA TCACAGAGCT CCTGGAGCAG AATGGAGGCT ACGGGAGCGC CACACTGTCC
CGAGGGCCAC CCCCTCCCGT GGGCCCCGCT GGCAGTGGCT ACAGTGGAGG CCTGGCGCTG
GCGGAGTACG ACCAGCCGTA TGGCGGCTTC CTGGACATTC TGCGGGGTGG GACAGAGCGG
CTCTTCACCA ACCTCAAGGA CACCTCCTCC AAGGTCATCC AGTCCGTCGC TAATTATGCA
AAGGGTGACC TGGACATATC TTACATCACA TCCAGAATTG CAGTGATGTC ATTCCCAGCA
GAAGGTGTGG AGTCAGCGCT CAAAAACAAC ATCGAAGATG TGCGGTTGTT CCTGGACTCC
AAGCACCCAG GGCACTATGC CGTCTACAAC CTGTCCCCGA GGACCTACCG GCCCTCCAGG
TTCCACAACC GGGTCTCCGA GTGTGGCTGG GCAGCACGGC GGGCCCCACA CCTGCACACC
CTGTACAACA TCTGCAGGAA CATGCACGCC TGGCTGCGGC AGGACCACAA GAACGTCTGC
GTCGTGCACT GCATGGACGG GAGAGCCGCG TCTGCTGTGG CCGTCTGCTC CTTCCTGTGC
TTCTGCCGTC TCTTCAGCAC CGCGGAGGCC GCCGTGTACA TGTTCAGCAT GAAGCGCTGC
CCACCAGGCA TCTGGCCATC CCACAAAAGG TACATCGAGT ACATGTGTGA CATGGTGGCG
GAGGAGCCCA TCACACCCCA CAGCAAGCCC ATCCTGGTGA GGGCCGTGGT CATGACACCC
GTGCCGCTGT TCAGCAAGCA GAGGAGCGGC TGCAGGCCCT TCTGCGAGGT CTACGTGGGG
GACGAGCGTG TGGCCAGCAC CTCCCAGGAG TACGACAAGA TGCGGGACTT TAAGATTGAA
GATGGCAAAG CGGTGATTCC CCTGGGCGTC ACGGTGCAAG GAGACGTGCT CATCGTCATC
TATCACGCCC GGTCCACTCT GGGCGGCCGG CTGCAGGCCA AGATGGCATC CATGAAGATG
TTCCAGATTC AGTTCCACAC GGGGTTTGTG CCTCGGAACG CCACCACTGT GAAATTTGCC
AAGTATGACC TGGACGCGTG TGACATTCAA GAAAAATACC CGGATTTATT TCAAGTGAAC
CTGGAAGTGG AGGTGGAGCC CAGGGACAGG CCGAGCCGGG AAGCCCCACC ATGGGAGAAC
TCGAGCATGA GGGGGCTGAA CCCCAAAATC CTGTTTTCCA GCCGGGAGGA GCAGCAAGAC
ATTCTGTCTA AGTTTGGGAA GCCGGAGCTT CCCCGGCAGC CTGGCTCCAC GGCTCAGTAT
GATGCTGGGG CAGGGTCCCC GGAAGCCGAA CCCACAGACT CTGACTCACC GCCAAGCAGC
AGCGCGGACG CCAGTCGCTT CCTGCACACG CTGGACTGGC AGGAAGAGAA GGAGGCAGAG
ACTGGTGCAG AAAATGCCTC TTCCAAGGAG AGCGAGTCTG CCCTGATGGA GGACAGAGAC
GAGAGTGAGG TGTCAGATGA AGGGGGATCC CCGATCTCCA GCGAGGGCCA GGAACCCAGG
GCCGACCCAG AGCCCCCCGG CCTGGCAGCA GGGCTGGTGC AGCAGGACTT GGTTTTTGAG
GTGGAGACAC CGGCTGTGCT GCCAGAGCCT GTGCCACAGG AAGACGGGGT CGACCTCCTG
GGCCTGCACT CCGAGGTGGG CGCAGGGCCA GCTGTACCCC CGCAGGCCTG CAAGGCCCCC
TCCAGCAACA CCGACCTGCT CAGCTGCCTC CTTGGGCCCC CTGAGGCCGC CTCCCAGGGG
CCCCCGGAGG ATCTGCTCAG CGAGGACCCG CTGCTCCTGG CAAGCCCGGC CCCTCCCCTG
AGCGTGCAGA GCACCCCAAG AGGAGGGCCC CCTGCCGCTG CTGACCCCTT TGGCCCGCTT
CTGCCGTCTT CAGGCAACAA CTCCCAGCCC TGCTCCAATC CTGATCTCTT CGGCGAATTT
CTCAATTCGG ACTCTGTGAC CGTCCCACCA TCCTTCCCGT CTGCCCACAG TGCTCCGCCC
CCATCCTGCA GCGCCGACTT CCTGCACCTG GGGGATCTGC CAGGAGAGCC CAGCAAGATG
ACAGCCTCGT CCAGCAACCC AGACCTGCTG GGAGGATGGG CTGCCTGGAC CGAGACTGCA
GCATCGGCAG TGGCCCCCAC GCCAGCCACA GAAGGCCCCC TCTTCTCTCC TGGAGGTCAG
CCGGCCCCTT GTGGCTCTCA GGCCAGCTGG ACCAAGTCTC AGAACCCGGA CCCATTTGCT
GACCTTGGCG ACCTCAGCTC CGGCCTCCAA GACCCCCAAG CCCAGAGCAC AGTGAGCCCA
AGGGGACAGC GTGTCTGCAC CTGTTCCAGG CGACTGCCAA CTGGCAAGCT AAAACCGGGA
GTTGCTGACA CTGGCACTGC TGCCAGCCCC CACCGGCATT GTGGCTCACC AGCTGGATTC
CCTCCTGGGG GCTTCATTCC CAAAACGGCC ACCACGCCCA AAGGCAGCAG CTCCTGGCAG
ACAAGTCGGC CGCCAGCCCA GGGCGCCTCA TGGCCCCCTC AGGCCAAGCC GCCCCCCAAA
GCCTGCACAC AGCCAAGGCC TAACTATGCC TCGAACTTCA GTGTGATCGG GGCGCGGGAG
GAGCGGGGGG TCCGCGCACC CAGCTTTGCT CAAAAGCCAA AAGTCTCTGA GAACGACTTT
GAAGATCTGT TGTCCAATCA AGGCTTCTCC TCCAGGTCTG ACAAGAAAGG GCCAAAGACC
ATTGCAGAGA TGAGGAAGCA GGACCTGGCT AAAGACACGG ACCCACTCAA GCTGAAGCTC
CTGGACTGGA TTGAGGGCAA GGAGCGGAAC ATCCGGGCCC TGCTGTCCAC GCTGCACACA
GTGCTGTGGG ACGGGGAGAG CCGCTGGACG CCCGTGGGCA TGGCCGACCT GGTGGCTCCG
GAGCAAGTGA AGAAGCACTA TCGCCGCGCG GTGCTGGCTG TGCACCCCGA CAAGGCTGCG
GGGCAGCCGT ACGAGCAGCA CGCCAAGATG ATCTTCATGG AGCTGAATGA CGCCTGGTCG
GAGTTTGAGA ACCAGGGCTC CCGGCCCCTC TTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24069
Clone ID Related Accession (Same CDS sequence) NM_005255.3
Accession Version NM_005255.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3936bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-15
Organism Homo sapiens(Human)
Product cyclin-G-associated kinase isoform 1
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA680073.1, D88435.1, BC000816.1, AA287110.1, AI138676.1 and AW050946.1. On Dec 25, 2015 this sequence version replaced NM_005255.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D88435.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATGTG
GGGAGTGGCA GAGAGTATGC ATTAAAGAGG CTATTATCCA ATGAAGAGGA AAAGAACAGA
GCCATCATTC AAGAAGTTTG CTTCATGAAA AAGCTTTCCG GCCACCCGAA CATTGTCCAG
TTTTGTTCTG CAGCGTCTAT AGGAAAAGAG GAGTCAGACA CGGGGCAGGC TGAGTTCCTC
TTGCTCACAG AGCTCTGTAA AGGGCAGCTG GTGGAATTTT TGAAGAAAAT GGAATCTCGA
GGCCCCCTTT CGTGCGACAC GGTTCTGAAG ATCTTCTACC AGACGTGCCG CGCCGTGCAG
CACATGCACC GGCAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA GAACTTGTTG
CTTAGTAACC AAGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACGAC CATCTCGCAC
TACCCTGACT ACAGCTGGAG CGCCCAGAGG CGAGCCCTGG TGGAGGAAGA GATCACGAGG
AATACAACAC CAATGTATAG AACACCAGAA ATCATAGACT TGTATTCCAA CTTCCCGATC
GGCGAGAAGC AGGATATCTG GGCCCTGGGC TGCATCTTGT ACCTGCTGTG CTTCCGGCAG
CACCCTTTTG AGGATGGAGC GAAACTTCGA ATAGTCAATG GGAAGTACTC GATCCCCCCG
CACGACACGC AGTACACGGT CTTCCACAGC CTCATCCGCG CCATGCTGCA GGTGAACCCG
GAGGAGCGGC TGTCCATCGC CGAGGTGGTG CACCAGCTGC AGGAGATCGC GGCCGCCCGC
AACGTGAACC CCAAGTCTCC CATCACAGAG CTCCTGGAGC AGAATGGAGG CTACGGGAGC
GCCACACTGT CCCGAGGGCC ACCCCCTCCC GTGGGCCCCG CTGGCAGTGG CTACAGTGGA
GGCCTGGCGC TGGCGGAGTA CGACCAGCCG TATGGCGGCT TCCTGGACAT TCTGCGGGGT
GGGACAGAGC GGCTCTTCAC CAACCTCAAG GACACCTCCT CCAAGGTCAT CCAGTCCGTC
GCTAATTATG CAAAGGGTGA CCTGGACATA TCTTACATCA CATCCAGAAT TGCAGTGATG
TCATTCCCAG CAGAAGGTGT GGAGTCAGCG CTCAAAAACA ACATCGAAGA TGTGCGGTTG
TTCCTGGACT CCAAGCACCC AGGGCACTAT GCCGTCTACA ACCTGTCCCC GAGGACCTAC
CGGCCCTCCA GGTTCCACAA CCGGGTCTCC GAGTGTGGCT GGGCAGCACG GCGGGCCCCA
CACCTGCACA CCCTGTACAA CATCTGCAGG AACATGCACG CCTGGCTGCG GCAGGACCAC
AAGAACGTCT GCGTCGTGCA CTGCATGGAC GGGAGAGCCG CGTCTGCTGT GGCCGTCTGC
TCCTTCCTGT GCTTCTGCCG TCTCTTCAGC ACCGCGGAGG CCGCCGTGTA CATGTTCAGC
ATGAAGCGCT GCCCACCAGG CATCTGGCCA TCCCACAAAA GGTACATCGA GTACATGTGT
GACATGGTGG CGGAGGAGCC CATCACACCC CACAGCAAGC CCATCCTGGT GAGGGCCGTG
GTCATGACAC CCGTGCCGCT GTTCAGCAAG CAGAGGAGCG GCTGCAGGCC CTTCTGCGAG
GTCTACGTGG GGGACGAGCG TGTGGCCAGC ACCTCCCAGG AGTACGACAA GATGCGGGAC
TTTAAGATTG AAGATGGCAA AGCGGTGATT CCCCTGGGCG TCACGGTGCA AGGAGACGTG
CTCATCGTCA TCTATCACGC CCGGTCCACT CTGGGCGGCC GGCTGCAGGC CAAGATGGCA
TCCATGAAGA TGTTCCAGAT TCAGTTCCAC ACGGGGTTTG TGCCTCGGAA CGCCACCACT
GTGAAATTTG CCAAGTATGA CCTGGACGCG TGTGACATTC AAGAAAAATA CCCGGATTTA
TTTCAAGTGA ACCTGGAAGT GGAGGTGGAG CCCAGGGACA GGCCGAGCCG GGAAGCCCCA
CCATGGGAGA ACTCGAGCAT GAGGGGGCTG AACCCCAAAA TCCTGTTTTC CAGCCGGGAG
GAGCAGCAAG ACATTCTGTC TAAGTTTGGG AAGCCGGAGC TTCCCCGGCA GCCTGGCTCC
ACGGCTCAGT ATGATGCTGG GGCAGGGTCC CCGGAAGCCG AACCCACAGA CTCTGACTCA
CCGCCAAGCA GCAGCGCGGA CGCCAGTCGC TTCCTGCACA CGCTGGACTG GCAGGAAGAG
AAGGAGGCAG AGACTGGTGC AGAAAATGCC TCTTCCAAGG AGAGCGAGTC TGCCCTGATG
GAGGACAGAG ACGAGAGTGA GGTGTCAGAT GAAGGGGGAT CCCCGATCTC CAGCGAGGGC
CAGGAACCCA GGGCCGACCC AGAGCCCCCC GGCCTGGCAG CAGGGCTGGT GCAGCAGGAC
TTGGTTTTTG AGGTGGAGAC ACCGGCTGTG CTGCCAGAGC CTGTGCCACA GGAAGACGGG
GTCGACCTCC TGGGCCTGCA CTCCGAGGTG GGCGCAGGGC CAGCTGTACC CCCGCAGGCC
TGCAAGGCCC CCTCCAGCAA CACCGACCTG CTCAGCTGCC TCCTTGGGCC CCCTGAGGCC
GCCTCCCAGG GGCCCCCGGA GGATCTGCTC AGCGAGGACC CGCTGCTCCT GGCAAGCCCG
GCCCCTCCCC TGAGCGTGCA GAGCACCCCA AGAGGAGGGC CCCCTGCCGC TGCTGACCCC
TTTGGCCCGC TTCTGCCGTC TTCAGGCAAC AACTCCCAGC CCTGCTCCAA TCCTGATCTC
TTCGGCGAAT TTCTCAATTC GGACTCTGTG ACCGTCCCAC CATCCTTCCC GTCTGCCCAC
AGTGCTCCGC CCCCATCCTG CAGCGCCGAC TTCCTGCACC TGGGGGATCT GCCAGGAGAG
CCCAGCAAGA TGACAGCCTC GTCCAGCAAC CCAGACCTGC TGGGAGGATG GGCTGCCTGG
ACCGAGACTG CAGCATCGGC AGTGGCCCCC ACGCCAGCCA CAGAAGGCCC CCTCTTCTCT
CCTGGAGGTC AGCCGGCCCC TTGTGGCTCT CAGGCCAGCT GGACCAAGTC TCAGAACCCG
GACCCATTTG CTGACCTTGG CGACCTCAGC TCCGGCCTCC AAGGCTCACC AGCTGGATTC
CCTCCTGGGG GCTTCATTCC CAAAACGGCC ACCACGCCCA AAGGCAGCAG CTCCTGGCAG
ACAAGTCGGC CGCCAGCCCA GGGCGCCTCA TGGCCCCCTC AGGCCAAGCC GCCCCCCAAA
GCCTGCACAC AGCCAAGGCC TAACTATGCC TCGAACTTCA GTGTGATCGG GGCGCGGGAG
GAGCGGGGGG TCCGCGCACC CAGCTTTGCT CAAAAGCCAA AAGTCTCTGA GAACGACTTT
GAAGATCTGT TGTCCAATCA AGGCTTCTCC TCCAGGTCTG ACAAGAAAGG GCCAAAGACC
ATTGCAGAGA TGAGGAAGCA GGACCTGGCT AAAGACACGG ACCCACTCAA GCTGAAGCTC
CTGGACTGGA TTGAGGGCAA GGAGCGGAAC ATCCGGGCCC TGCTGTCCAC GCTGCACACA
GTGCTGTGGG ACGGGGAGAG CCGCTGGACG CCCGTGGGCA TGGCCGACCT GGTGGCTCCG
GAGCAAGTGA AGAAGCACTA TCGCCGCGCG GTGCTGGCTG TGCACCCCGA CAAGGCTGCG
GGGCAGCCGT ACGAGCAGCA CGCCAAGATG ATCTTCATGG AGCTGAATGA CGCCTGGTCG
GAGTTTGAGA ACCAGGGCTC CCGGCCCCTC TTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005246.2
CDS204..4139
Misc Feature(1)129..131
Misc Feature(2)207..209
Misc Feature(3)207..209
Misc Feature(4)249..251
Misc Feature(5)318..1163
Misc Feature(6)324..1130
Misc Feature(7)order(339..353,363..365,402..404,408..410,498..500,570..581,588..590,594..596,720..722,726..728,732..737,741..743,774..776,783..785,867..878)
Misc Feature(8)order(339..353,363..365,402..404,408..410,498..500,570..581,588..590,720..722,726..728,732..737,741..743,774..776)
Misc Feature(9)order(351..353,588..590,594..596,720..722,726..728,732..734,783..785,867..878)
Misc Feature(10)order(771..863,867..878)
Misc Feature(11)1569..1571
Misc Feature(12)1911..2327
Misc Feature(13)2511..2513
Misc Feature(14)2529..2531
Misc Feature(15)2550..2552
Misc Feature(16)2583..2585
Misc Feature(17)2634..2636
Misc Feature(18)2679..2681
Misc Feature(19)2688..2690
Misc Feature(20)2703..2705
Misc Feature(21)3018..3020
Misc Feature(22)3489..3491
Misc Feature(23)3570..3572
Misc Feature(24)3729..3731
Misc Feature(25)3756..3758
Misc Feature(26)<3981..4115
Misc Feature(27)order(4026..4034,4068..4070,4077..4082,4089..4094)
Exon (1)1..348
Exon (2)349..410
Exon (3)411..470
Exon (4)471..585
Exon (5)586..728
Exon (6)729..854
Exon (7)855..944
Exon (8)945..1080
Exon (9)1081..1193
Exon (10)1194..1284
Exon (11)1285..1408
Exon (12)1409..1458
Exon (13)1459..1607
Exon (14)1608..1730
Exon (15)1731..1864
Exon (16)1865..2059
Exon (17)2060..2177
Exon (18)2178..2257
Exon (19)2258..2451
Exon (20)2452..2598
Exon (21)2599..3075
Exon (22)3076..3246
Exon (23)3247..3369
Exon (24)3370..3486
Exon (25)3487..3711
Exon (26)3712..3860
Exon (27)3861..4037
Exon (28)4038..4537
Translation

Target ORF information:

RefSeq Version NM_005255.3
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin G associated kinase (GAK), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005255.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATGTG
GGGAGTGGCA GAGAGTATGC ATTAAAGAGG CTATTATCCA ATGAAGAGGA AAAGAACAGA
GCCATCATTC AAGAAGTTTG CTTCATGAAA AAGCTTTCCG GCCACCCGAA CATTGTCCAG
TTTTGTTCTG CAGCGTCTAT AGGAAAAGAG GAGTCAGACA CGGGGCAGGC TGAGTTCCTC
TTGCTCACAG AGCTCTGTAA AGGGCAGCTG GTGGAATTTT TGAAGAAAAT GGAATCTCGA
GGCCCCCTTT CGTGCGACAC GGTTCTGAAG ATCTTCTACC AGACGTGCCG CGCCGTGCAG
CACATGCACC GGCAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA GAACTTGTTG
CTTAGTAACC AAGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACGAC CATCTCGCAC
TACCCTGACT ACAGCTGGAG CGCCCAGAGG CGAGCCCTGG TGGAGGAAGA GATCACGAGG
AATACAACAC CAATGTATAG AACACCAGAA ATCATAGACT TGTATTCCAA CTTCCCGATC
GGCGAGAAGC AGGATATCTG GGCCCTGGGC TGCATCTTGT ACCTGCTGTG CTTCCGGCAG
CACCCTTTTG AGGATGGAGC GAAACTTCGA ATAGTCAATG GGAAGTACTC GATCCCCCCG
CACGACACGC AGTACACGGT CTTCCACAGC CTCATCCGCG CCATGCTGCA GGTGAACCCG
GAGGAGCGGC TGTCCATCGC CGAGGTGGTG CACCAGCTGC AGGAGATCGC GGCCGCCCGC
AACGTGAACC CCAAGTCTCC CATCACAGAG CTCCTGGAGC AGAATGGAGG CTACGGGAGC
GCCACACTGT CCCGAGGGCC ACCCCCTCCC GTGGGCCCCG CTGGCAGTGG CTACAGTGGA
GGCCTGGCGC TGGCGGAGTA CGACCAGCCG TATGGCGGCT TCCTGGACAT TCTGCGGGGT
GGGACAGAGC GGCTCTTCAC CAACCTCAAG GACACCTCCT CCAAGGTCAT CCAGTCCGTC
GCTAATTATG CAAAGGGTGA CCTGGACATA TCTTACATCA CATCCAGAAT TGCAGTGATG
TCATTCCCAG CAGAAGGTGT GGAGTCAGCG CTCAAAAACA ACATCGAAGA TGTGCGGTTG
TTCCTGGACT CCAAGCACCC AGGGCACTAT GCCGTCTACA ACCTGTCCCC GAGGACCTAC
CGGCCCTCCA GGTTCCACAA CCGGGTCTCC GAGTGTGGCT GGGCAGCACG GCGGGCCCCA
CACCTGCACA CCCTGTACAA CATCTGCAGG AACATGCACG CCTGGCTGCG GCAGGACCAC
AAGAACGTCT GCGTCGTGCA CTGCATGGAC GGGAGAGCCG CGTCTGCTGT GGCCGTCTGC
TCCTTCCTGT GCTTCTGCCG TCTCTTCAGC ACCGCGGAGG CCGCCGTGTA CATGTTCAGC
ATGAAGCGCT GCCCACCAGG CATCTGGCCA TCCCACAAAA GGTACATCGA GTACATGTGT
GACATGGTGG CGGAGGAGCC CATCACACCC CACAGCAAGC CCATCCTGGT GAGGGCCGTG
GTCATGACAC CCGTGCCGCT GTTCAGCAAG CAGAGGAGCG GCTGCAGGCC CTTCTGCGAG
GTCTACGTGG GGGACGAGCG TGTGGCCAGC ACCTCCCAGG AGTACGACAA GATGCGGGAC
TTTAAGATTG AAGATGGCAA AGCGGTGATT CCCCTGGGCG TCACGGTGCA AGGAGACGTG
CTCATCGTCA TCTATCACGC CCGGTCCACT CTGGGCGGCC GGCTGCAGGC CAAGATGGCA
TCCATGAAGA TGTTCCAGAT TCAGTTCCAC ACGGGGTTTG TGCCTCGGAA CGCCACCACT
GTGAAATTTG CCAAGTATGA CCTGGACGCG TGTGACATTC AAGAAAAATA CCCGGATTTA
TTTCAAGTGA ACCTGGAAGT GGAGGTGGAG CCCAGGGACA GGCCGAGCCG GGAAGCCCCA
CCATGGGAGA ACTCGAGCAT GAGGGGGCTG AACCCCAAAA TCCTGTTTTC CAGCCGGGAG
GAGCAGCAAG ACATTCTGTC TAAGTTTGGG AAGCCGGAGC TTCCCCGGCA GCCTGGCTCC
ACGGCTCAGT ATGATGCTGG GGCAGGGTCC CCGGAAGCCG AACCCACAGA CTCTGACTCA
CCGCCAAGCA GCAGCGCGGA CGCCAGTCGC TTCCTGCACA CGCTGGACTG GCAGGAAGAG
AAGGAGGCAG AGACTGGTGC AGAAAATGCC TCTTCCAAGG AGAGCGAGTC TGCCCTGATG
GAGGACAGAG ACGAGAGTGA GGTGTCAGAT GAAGGGGGAT CCCCGATCTC CAGCGAGGGC
CAGGAACCCA GGGCCGACCC AGAGCCCCCC GGCCTGGCAG CAGGGCTGGT GCAGCAGGAC
TTGGTTTTTG AGGTGGAGAC ACCGGCTGTG CTGCCAGAGC CTGTGCCACA GGAAGACGGG
GTCGACCTCC TGGGCCTGCA CTCCGAGGTG GGCGCAGGGC CAGCTGTACC CCCGCAGGCC
TGCAAGGCCC CCTCCAGCAA CACCGACCTG CTCAGCTGCC TCCTTGGGCC CCCTGAGGCC
GCCTCCCAGG GGCCCCCGGA GGATCTGCTC AGCGAGGACC CGCTGCTCCT GGCAAGCCCG
GCCCCTCCCC TGAGCGTGCA GAGCACCCCA AGAGGAGGGC CCCCTGCCGC TGCTGACCCC
TTTGGCCCGC TTCTGCCGTC TTCAGGCAAC AACTCCCAGC CCTGCTCCAA TCCTGATCTC
TTCGGCGAAT TTCTCAATTC GGACTCTGTG ACCGTCCCAC CATCCTTCCC GTCTGCCCAC
AGTGCTCCGC CCCCATCCTG CAGCGCCGAC TTCCTGCACC TGGGGGATCT GCCAGGAGAG
CCCAGCAAGA TGACAGCCTC GTCCAGCAAC CCAGACCTGC TGGGAGGATG GGCTGCCTGG
ACCGAGACTG CAGCATCGGC AGTGGCCCCC ACGCCAGCCA CAGAAGGCCC CCTCTTCTCT
CCTGGAGGTC AGCCGGCCCC TTGTGGCTCT CAGGCCAGCT GGACCAAGTC TCAGAACCCG
GACCCATTTG CTGACCTTGG CGACCTCAGC TCCGGCCTCC AAGGCTCACC AGCTGGATTC
CCTCCTGGGG GCTTCATTCC CAAAACGGCC ACCACGCCCA AAGGCAGCAG CTCCTGGCAG
ACAAGTCGGC CGCCAGCCCA GGGCGCCTCA TGGCCCCCTC AGGCCAAGCC GCCCCCCAAA
GCCTGCACAC AGCCAAGGCC TAACTATGCC TCGAACTTCA GTGTGATCGG GGCGCGGGAG
GAGCGGGGGG TCCGCGCACC CAGCTTTGCT CAAAAGCCAA AAGTCTCTGA GAACGACTTT
GAAGATCTGT TGTCCAATCA AGGCTTCTCC TCCAGGTCTG ACAAGAAAGG GCCAAAGACC
ATTGCAGAGA TGAGGAAGCA GGACCTGGCT AAAGACACGG ACCCACTCAA GCTGAAGCTC
CTGGACTGGA TTGAGGGCAA GGAGCGGAAC ATCCGGGCCC TGCTGTCCAC GCTGCACACA
GTGCTGTGGG ACGGGGAGAG CCGCTGGACG CCCGTGGGCA TGGCCGACCT GGTGGCTCCG
GAGCAAGTGA AGAAGCACTA TCGCCGCGCG GTGCTGGCTG TGCACCCCGA CAAGGCTGCG
GGGCAGCCGT ACGAGCAGCA CGCCAAGATG ATCTTCATGG AGCTGAATGA CGCCTGGTCG
GAGTTTGAGA ACCAGGGCTC CCGGCCCCTC TTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57911
Clone ID Related Accession (Same CDS sequence) NM_001318134.1
Accession Version NM_001318134.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3699bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-15
Organism Homo sapiens(Human)
Product cyclin-G-associated kinase isoform 3
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA680073.1, BC085005.1, AK299554.1, BC000816.1, AA287110.1, AI138676.1 and AW050946.1. On Dec 25, 2015 this sequence version replaced XM_011513433.1. Transcript Variant: This variant (3) lacks three consecutive exons in the 5' coding region but maintains the same reading frame as variant 1. The encoded isoform (3) has the same N- and C-termini, but is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC085005.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGGCAG CTGGTGGAAT TTTTGAAGAA AATGGAATCT
CGAGGCCCCC TTTCGTGCGA CACGGTTCTG AAGATCTTCT ACCAGACGTG CCGCGCCGTG
CAGCACATGC ACCGGCAGAA GCCGCCCATC ATCCACAGGG ACCTCAAGGT TGAGAACTTG
TTGCTTAGTA ACCAAGGGAC CATTAAGCTG TGTGACTTTG GCAGTGCCAC GACCATCTCG
CACTACCCTG ACTACAGCTG GAGCGCCCAG AGGCGAGCCC TGGTGGAGGA AGAGATCACG
AGGAATACAA CACCAATGTA TAGAACACCA GAAATCATAG ACTTGTATTC CAACTTCCCG
ATCGGCGAGA AGCAGGATAT CTGGGCCCTG GGCTGCATCT TGTACCTGCT GTGCTTCCGG
CAGCACCCTT TTGAGGATGG AGCGAAACTT CGAATAGTCA ATGGGAAGTA CTCGATCCCC
CCGCACGACA CGCAGTACAC GGTCTTCCAC AGCCTCATCC GCGCCATGCT GCAGGTGAAC
CCGGAGGAGC GGCTGTCCAT CGCCGAGGTG GTGCACCAGC TGCAGGAGAT CGCGGCCGCC
CGCAACGTGA ACCCCAAGTC TCCCATCACA GAGCTCCTGG AGCAGAATGG AGGCTACGGG
AGCGCCACAC TGTCCCGAGG GCCACCCCCT CCCGTGGGCC CCGCTGGCAG TGGCTACAGT
GGAGGCCTGG CGCTGGCGGA GTACGACCAG CCGTATGGCG GCTTCCTGGA CATTCTGCGG
GGTGGGACAG AGCGGCTCTT CACCAACCTC AAGGACACCT CCTCCAAGGT CATCCAGTCC
GTCGCTAATT ATGCAAAGGG TGACCTGGAC ATATCTTACA TCACATCCAG AATTGCAGTG
ATGTCATTCC CAGCAGAAGG TGTGGAGTCA GCGCTCAAAA ACAACATCGA AGATGTGCGG
TTGTTCCTGG ACTCCAAGCA CCCAGGGCAC TATGCCGTCT ACAACCTGTC CCCGAGGACC
TACCGGCCCT CCAGGTTCCA CAACCGGGTC TCCGAGTGTG GCTGGGCAGC ACGGCGGGCC
CCACACCTGC ACACCCTGTA CAACATCTGC AGGAACATGC ACGCCTGGCT GCGGCAGGAC
CACAAGAACG TCTGCGTCGT GCACTGCATG GACGGGAGAG CCGCGTCTGC TGTGGCCGTC
TGCTCCTTCC TGTGCTTCTG CCGTCTCTTC AGCACCGCGG AGGCCGCCGT GTACATGTTC
AGCATGAAGC GCTGCCCACC AGGCATCTGG CCATCCCACA AAAGGTACAT CGAGTACATG
TGTGACATGG TGGCGGAGGA GCCCATCACA CCCCACAGCA AGCCCATCCT GGTGAGGGCC
GTGGTCATGA CACCCGTGCC GCTGTTCAGC AAGCAGAGGA GCGGCTGCAG GCCCTTCTGC
GAGGTCTACG TGGGGGACGA GCGTGTGGCC AGCACCTCCC AGGAGTACGA CAAGATGCGG
GACTTTAAGA TTGAAGATGG CAAAGCGGTG ATTCCCCTGG GCGTCACGGT GCAAGGAGAC
GTGCTCATCG TCATCTATCA CGCCCGGTCC ACTCTGGGCG GCCGGCTGCA GGCCAAGATG
GCATCCATGA AGATGTTCCA GATTCAGTTC CACACGGGGT TTGTGCCTCG GAACGCCACC
ACTGTGAAAT TTGCCAAGTA TGACCTGGAC GCGTGTGACA TTCAAGAAAA ATACCCGGAT
TTATTTCAAG TGAACCTGGA AGTGGAGGTG GAGCCCAGGG ACAGGCCGAG CCGGGAAGCC
CCACCATGGG AGAACTCGAG CATGAGGGGG CTGAACCCCA AAATCCTGTT TTCCAGCCGG
GAGGAGCAGC AAGACATTCT GTCTAAGTTT GGGAAGCCGG AGCTTCCCCG GCAGCCTGGC
TCCACGGCTC AGTATGATGC TGGGGCAGGG TCCCCGGAAG CCGAACCCAC AGACTCTGAC
TCACCGCCAA GCAGCAGCGC GGACGCCAGT CGCTTCCTGC ACACGCTGGA CTGGCAGGAA
GAGAAGGAGG CAGAGACTGG TGCAGAAAAT GCCTCTTCCA AGGAGAGCGA GTCTGCCCTG
ATGGAGGACA GAGACGAGAG TGAGGTGTCA GATGAAGGGG GATCCCCGAT CTCCAGCGAG
GGCCAGGAAC CCAGGGCCGA CCCAGAGCCC CCCGGCCTGG CAGCAGGGCT GGTGCAGCAG
GACTTGGTTT TTGAGGTGGA GACACCGGCT GTGCTGCCAG AGCCTGTGCC ACAGGAAGAC
GGGGTCGACC TCCTGGGCCT GCACTCCGAG GTGGGCGCAG GGCCAGCTGT ACCCCCGCAG
GCCTGCAAGG CCCCCTCCAG CAACACCGAC CTGCTCAGCT GCCTCCTTGG GCCCCCTGAG
GCCGCCTCCC AGGGGCCCCC GGAGGATCTG CTCAGCGAGG ACCCGCTGCT CCTGGCAAGC
CCGGCCCCTC CCCTGAGCGT GCAGAGCACC CCAAGAGGAG GGCCCCCTGC CGCTGCTGAC
CCCTTTGGCC CGCTTCTGCC GTCTTCAGGC AACAACTCCC AGCCCTGCTC CAATCCTGAT
CTCTTCGGCG AATTTCTCAA TTCGGACTCT GTGACCGTCC CACCATCCTT CCCGTCTGCC
CACAGTGCTC CGCCCCCATC CTGCAGCGCC GACTTCCTGC ACCTGGGGGA TCTGCCAGGA
GAGCCCAGCA AGATGACAGC CTCGTCCAGC AACCCAGACC TGCTGGGAGG ATGGGCTGCC
TGGACCGAGA CTGCAGCATC GGCAGTGGCC CCCACGCCAG CCACAGAAGG CCCCCTCTTC
TCTCCTGGAG GTCAGCCGGC CCCTTGTGGC TCTCAGGCCA GCTGGACCAA GTCTCAGAAC
CCGGACCCAT TTGCTGACCT TGGCGACCTC AGCTCCGGCC TCCAAGGCTC ACCAGCTGGA
TTCCCTCCTG GGGGCTTCAT TCCCAAAACG GCCACCACGC CCAAAGGCAG CAGCTCCTGG
CAGACAAGTC GGCCGCCAGC CCAGGGCGCC TCATGGCCCC CTCAGGCCAA GCCGCCCCCC
AAAGCCTGCA CACAGCCAAG GCCTAACTAT GCCTCGAACT TCAGTGTGAT CGGGGCGCGG
GAGGAGCGGG GGGTCCGCGC ACCCAGCTTT GCTCAAAAGC CAAAAGTCTC TGAGAACGAC
TTTGAAGATC TGTTGTCCAA TCAAGGCTTC TCCTCCAGGT CTGACAAGAA AGGGCCAAAG
ACCATTGCAG AGATGAGGAA GCAGGACCTG GCTAAAGACA CGGACCCACT CAAGCTGAAG
CTCCTGGACT GGATTGAGGG CAAGGAGCGG AACATCCGGG CCCTGCTGTC CACGCTGCAC
ACAGTGCTGT GGGACGGGGA GAGCCGCTGG ACGCCCGTGG GCATGGCCGA CCTGGTGGCT
CCGGAGCAAG TGAAGAAGCA CTATCGCCGC GCGGTGCTGG CTGTGCACCC CGACAAGGCT
GCGGGGCAGC CGTACGAGCA GCACGCCAAG ATGATCTTCA TGGAGCTGAA TGACGCCTGG
TCGGAGTTTG AGAACCAGGG CTCCCGGCCC CTCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001305063.1
CDS204..3902
Misc Feature(1)129..131
Misc Feature(2)207..209
Misc Feature(3)207..209
Misc Feature(4)249..251
Misc Feature(5)<339..926
Misc Feature(6)<360..893
Misc Feature(7)1674..2090
Misc Feature(8)<3744..3878
Misc Feature(9)order(3789..3797,3831..3833,3840..3845,3852..3857)
Exon (1)1..348
Exon (2)349..491
Exon (3)492..617
Exon (4)618..707
Exon (5)708..843
Exon (6)844..956
Exon (7)957..1047
Exon (8)1048..1171
Exon (9)1172..1221
Exon (10)1222..1370
Exon (11)1371..1493
Exon (12)1494..1627
Exon (13)1628..1822
Exon (14)1823..1940
Exon (15)1941..2020
Exon (16)2021..2214
Exon (17)2215..2361
Exon (18)2362..2838
Exon (19)2839..3009
Exon (20)3010..3132
Exon (21)3133..3249
Exon (22)3250..3474
Exon (23)3475..3623
Exon (24)3624..3800
Exon (25)3801..4300
Translation

Target ORF information:

RefSeq Version NM_001318134.1
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin G associated kinase (GAK), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001318134.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGGCAG CTGGTGGAAT TTTTGAAGAA AATGGAATCT
CGAGGCCCCC TTTCGTGCGA CACGGTTCTG AAGATCTTCT ACCAGACGTG CCGCGCCGTG
CAGCACATGC ACCGGCAGAA GCCGCCCATC ATCCACAGGG ACCTCAAGGT TGAGAACTTG
TTGCTTAGTA ACCAAGGGAC CATTAAGCTG TGTGACTTTG GCAGTGCCAC GACCATCTCG
CACTACCCTG ACTACAGCTG GAGCGCCCAG AGGCGAGCCC TGGTGGAGGA AGAGATCACG
AGGAATACAA CACCAATGTA TAGAACACCA GAAATCATAG ACTTGTATTC CAACTTCCCG
ATCGGCGAGA AGCAGGATAT CTGGGCCCTG GGCTGCATCT TGTACCTGCT GTGCTTCCGG
CAGCACCCTT TTGAGGATGG AGCGAAACTT CGAATAGTCA ATGGGAAGTA CTCGATCCCC
CCGCACGACA CGCAGTACAC GGTCTTCCAC AGCCTCATCC GCGCCATGCT GCAGGTGAAC
CCGGAGGAGC GGCTGTCCAT CGCCGAGGTG GTGCACCAGC TGCAGGAGAT CGCGGCCGCC
CGCAACGTGA ACCCCAAGTC TCCCATCACA GAGCTCCTGG AGCAGAATGG AGGCTACGGG
AGCGCCACAC TGTCCCGAGG GCCACCCCCT CCCGTGGGCC CCGCTGGCAG TGGCTACAGT
GGAGGCCTGG CGCTGGCGGA GTACGACCAG CCGTATGGCG GCTTCCTGGA CATTCTGCGG
GGTGGGACAG AGCGGCTCTT CACCAACCTC AAGGACACCT CCTCCAAGGT CATCCAGTCC
GTCGCTAATT ATGCAAAGGG TGACCTGGAC ATATCTTACA TCACATCCAG AATTGCAGTG
ATGTCATTCC CAGCAGAAGG TGTGGAGTCA GCGCTCAAAA ACAACATCGA AGATGTGCGG
TTGTTCCTGG ACTCCAAGCA CCCAGGGCAC TATGCCGTCT ACAACCTGTC CCCGAGGACC
TACCGGCCCT CCAGGTTCCA CAACCGGGTC TCCGAGTGTG GCTGGGCAGC ACGGCGGGCC
CCACACCTGC ACACCCTGTA CAACATCTGC AGGAACATGC ACGCCTGGCT GCGGCAGGAC
CACAAGAACG TCTGCGTCGT GCACTGCATG GACGGGAGAG CCGCGTCTGC TGTGGCCGTC
TGCTCCTTCC TGTGCTTCTG CCGTCTCTTC AGCACCGCGG AGGCCGCCGT GTACATGTTC
AGCATGAAGC GCTGCCCACC AGGCATCTGG CCATCCCACA AAAGGTACAT CGAGTACATG
TGTGACATGG TGGCGGAGGA GCCCATCACA CCCCACAGCA AGCCCATCCT GGTGAGGGCC
GTGGTCATGA CACCCGTGCC GCTGTTCAGC AAGCAGAGGA GCGGCTGCAG GCCCTTCTGC
GAGGTCTACG TGGGGGACGA GCGTGTGGCC AGCACCTCCC AGGAGTACGA CAAGATGCGG
GACTTTAAGA TTGAAGATGG CAAAGCGGTG ATTCCCCTGG GCGTCACGGT GCAAGGAGAC
GTGCTCATCG TCATCTATCA CGCCCGGTCC ACTCTGGGCG GCCGGCTGCA GGCCAAGATG
GCATCCATGA AGATGTTCCA GATTCAGTTC CACACGGGGT TTGTGCCTCG GAACGCCACC
ACTGTGAAAT TTGCCAAGTA TGACCTGGAC GCGTGTGACA TTCAAGAAAA ATACCCGGAT
TTATTTCAAG TGAACCTGGA AGTGGAGGTG GAGCCCAGGG ACAGGCCGAG CCGGGAAGCC
CCACCATGGG AGAACTCGAG CATGAGGGGG CTGAACCCCA AAATCCTGTT TTCCAGCCGG
GAGGAGCAGC AAGACATTCT GTCTAAGTTT GGGAAGCCGG AGCTTCCCCG GCAGCCTGGC
TCCACGGCTC AGTATGATGC TGGGGCAGGG TCCCCGGAAG CCGAACCCAC AGACTCTGAC
TCACCGCCAA GCAGCAGCGC GGACGCCAGT CGCTTCCTGC ACACGCTGGA CTGGCAGGAA
GAGAAGGAGG CAGAGACTGG TGCAGAAAAT GCCTCTTCCA AGGAGAGCGA GTCTGCCCTG
ATGGAGGACA GAGACGAGAG TGAGGTGTCA GATGAAGGGG GATCCCCGAT CTCCAGCGAG
GGCCAGGAAC CCAGGGCCGA CCCAGAGCCC CCCGGCCTGG CAGCAGGGCT GGTGCAGCAG
GACTTGGTTT TTGAGGTGGA GACACCGGCT GTGCTGCCAG AGCCTGTGCC ACAGGAAGAC
GGGGTCGACC TCCTGGGCCT GCACTCCGAG GTGGGCGCAG GGCCAGCTGT ACCCCCGCAG
GCCTGCAAGG CCCCCTCCAG CAACACCGAC CTGCTCAGCT GCCTCCTTGG GCCCCCTGAG
GCCGCCTCCC AGGGGCCCCC GGAGGATCTG CTCAGCGAGG ACCCGCTGCT CCTGGCAAGC
CCGGCCCCTC CCCTGAGCGT GCAGAGCACC CCAAGAGGAG GGCCCCCTGC CGCTGCTGAC
CCCTTTGGCC CGCTTCTGCC GTCTTCAGGC AACAACTCCC AGCCCTGCTC CAATCCTGAT
CTCTTCGGCG AATTTCTCAA TTCGGACTCT GTGACCGTCC CACCATCCTT CCCGTCTGCC
CACAGTGCTC CGCCCCCATC CTGCAGCGCC GACTTCCTGC ACCTGGGGGA TCTGCCAGGA
GAGCCCAGCA AGATGACAGC CTCGTCCAGC AACCCAGACC TGCTGGGAGG ATGGGCTGCC
TGGACCGAGA CTGCAGCATC GGCAGTGGCC CCCACGCCAG CCACAGAAGG CCCCCTCTTC
TCTCCTGGAG GTCAGCCGGC CCCTTGTGGC TCTCAGGCCA GCTGGACCAA GTCTCAGAAC
CCGGACCCAT TTGCTGACCT TGGCGACCTC AGCTCCGGCC TCCAAGGCTC ACCAGCTGGA
TTCCCTCCTG GGGGCTTCAT TCCCAAAACG GCCACCACGC CCAAAGGCAG CAGCTCCTGG
CAGACAAGTC GGCCGCCAGC CCAGGGCGCC TCATGGCCCC CTCAGGCCAA GCCGCCCCCC
AAAGCCTGCA CACAGCCAAG GCCTAACTAT GCCTCGAACT TCAGTGTGAT CGGGGCGCGG
GAGGAGCGGG GGGTCCGCGC ACCCAGCTTT GCTCAAAAGC CAAAAGTCTC TGAGAACGAC
TTTGAAGATC TGTTGTCCAA TCAAGGCTTC TCCTCCAGGT CTGACAAGAA AGGGCCAAAG
ACCATTGCAG AGATGAGGAA GCAGGACCTG GCTAAAGACA CGGACCCACT CAAGCTGAAG
CTCCTGGACT GGATTGAGGG CAAGGAGCGG AACATCCGGG CCCTGCTGTC CACGCTGCAC
ACAGTGCTGT GGGACGGGGA GAGCCGCTGG ACGCCCGTGG GCATGGCCGA CCTGGTGGCT
CCGGAGCAAG TGAAGAAGCA CTATCGCCGC GCGGTGCTGG CTGTGCACCC CGACAAGGCT
GCGGGGCAGC CGTACGAGCA GCACGCCAAG ATGATCTTCA TGGAGCTGAA TGACGCCTGG
TCGGAGTTTG AGAACCAGGG CTCCCGGCCC CTCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu38320
Clone ID Related Accession (Same CDS sequence) XM_005272268.2
Accession Version XM_005272268.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3903bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product cyclin-G-associated kinase isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005272268.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATGTG
GGGAGTGGCA GAGAGTATGC ATTAAAGAGG CTATTATCCA ATGAAGAGGA AAAGAACAGA
GCCATCATTC AAGAAGTTTG CTTCATGAAA AAGCTTTCCG GCCACCCGAA CATTGTCCAG
TTTTGTTCTG CAGCGTCTAT AGGAAAAGAG GAGTCAGACA CGGGGCAGGC TGAGTTCCTC
TTGCTCACAG AGCTCTGTAA AGGGCAGCTG GTGGAATTTT TGAAGAAAAT GGAATCTCGA
GGCCCCCTTT CGTGCGACAC GGTTCTGAAG ATCTTCTACC AGACGTGCCG CGCCGTGCAG
CACATGCACC GGCAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA GAACTTGTTG
CTTAGTAACC AAGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACGAC CATCTCGCAC
TACCCTGACT ACAGCTGGAG CGCCCAGAGG CGAGCCCTGG TGGAGGAAGA GATCACGAGG
AATACAACAC CAATGTATAG AACACCAGAA ATCATAGACT TGTATTCCAA CTTCCCGATC
GGCGAGAAGC AGGATATCTG GGCCCTGGGC TGCATCTTGT ACCTGCTGTG CTTCCGGCAG
CACCCTTTTG AGGATGGAGC GAAACTTCGA ATAGTCAATG GGAAGTACTC GATCCCCCCG
CACGACACGC AGTACACGGT CTTCCACAGC CTCATCCGCG CCATGCTGCA GGTGAACCCG
GAGGAGCGGC TGTCCATCGC CGAGGTGGTG CACCAGCTGC AGGAGATCGC GGCCGCCCGC
AACGTGAACC CCAAGTCTCC CATCACAGAG CTCCTGGAGC AGAATGGAGG CTACGGGAGC
GCCACACTGT CCCGAGGGCC ACCCCCTCCC GTGGGCCCCG CTGGCAGTGG CTACAGTGGA
GGCCTGGCGC TGGCGGAGTA CGACCAGCCG TATGGCGGCT TCCTGGACAT TCTGCGGGGT
GGGACAGAGC GGCTCTTCAC CAACCTCAAG GACACCTCCT CCAAGGTCAT CCAGTCCGTC
GCTAATTATG CAAAGGGTGA CCTGGACATA TCTTACATCA CATCCAGAAT TGCAGTGATG
TCATTCCCAG CAGAAGGTGT GGAGTCAGCG CTCAAAAACA ACATCGAAGA TGTGCGGTTG
TTCCTGGACT CCAAGCACCC AGGGCACTAT GCCGTCTACA ACCTGTCCCC GAGGACCTAC
CGGCCCTCCA GGTTCCACAA CCGGGTCTCC GAGTGTGGCT GGGCAGCACG GCGGGCCCCA
CACCTGCACA CCCTGTACAA CATCTGCAGG AACATGCACG CCTGGCTGCG GCAGGACCAC
AAGAACGTCT GCGTCGTGCA CTGCATGGAC GGGAGAGCCG CGTCTGCTGT GGCCGTCTGC
TCCTTCCTGT GCTTCTGCCG TCTCTTCAGC ACCGCGGAGG CCGCCGTGTA CATGTTCAGC
ATGAAGCGCT GCCCACCAGG CATCTGGCCA TCCCACAAAA GGTACATCGA GTACATGTGT
GACATGGTGG CGGAGGAGCC CATCACACCC CACAGCAAGC CCATCCTGGT GAGGGCCGTG
GTCATGACAC CCGTGCCGCT GTTCAGCAAG CAGAGGAGCG GCTGCAGGCC CTTCTGCGAG
GTCTACGTGG GGGACGAGCG TGTGGCCAGC ACCTCCCAGG AGTACGACAA GATGCGGGAC
TTTAAGATTG AAGATGGCAA AGCGGTGATT CCCCTGGGCG TCACGGTGCA AGGAGACGTG
CTCATCGTCA TCTATCACGC CCGGTCCACT CTGGGCGGCC GGCTGCAGGC CAAGATGGCA
TCCATGAAGA TGTTCCAGAT TCAGTTCCAC ACGGGGTTTG TGCCTCGGAA CGCCACCACT
GTGAAATTTG CCAAGTATGA CCTGGACGCG TGTGACATTC AAGAAAAATA CCCGGATTTA
TTTCAAGTGA ACCTGGAAGT GGAGGTGGAG CCCAGGGACA GGCCGAGCCG GGAAGCCCCA
CCATGGGAGA ACTCGAGCAT GAGGGGGCTG AACCCCAAAA TCCTGTTTTC CAGCCGGGAG
GAGCAGCAAG ACATTCTGTC TAAGTTTGGG AAGCCGGAGC TTCCCCGGCA GCCTGGCTCC
ACGGCTCAGT ATGATGCTGG GGCAGGGTCC CCGGAAGCCG AACCCACAGA CTCTGACTCA
CCGCCAAGCA GCAGCGCGGA CGCCAGTCGC TTCCTGCACA CGCTGGACTG GCAGGAAGAG
AAGGAGGCAG AGACTGGTGC AGAAAATGCC TCTTCCAAGG AGAGCGAGTC TGCCCTGATG
GAGGACAGAG ACGAGAGTGA GGTGTCAGAT GAAGGGGGAT CCCCGATCTC CAGCGAGGGC
CAGGAACCCA GGGCCGACCC AGAGCCCCCC GGCCTGGCAG CAGGGCTGGT GCAGCAGGAC
TTGGTTTTTG AGGTGGAGAC ACCGGCTGTG CTGCCAGAGC CTGTGCCACA GGAAGACGGG
GTCGACCTCC TGGGCCTGCA CTCCGAGGTG GGCGCAGGGC CAGCTGTACC CCCGCAGGCC
TGCAAGGCCC CCTCCAGCAA CACCGACCTG CTCAGCTGCC TCCTTGGGCC CCCTGAGGCC
GCCTCCCAGG GGCCCCCGGA GGATCTGCTC AGCGAGGACC CGCTGCTCCT GGCAAGCCCG
GCCCCTCCCC TGAGCGTGCA GAGCACCCCA AGAGGAGGGC CCCCTGCCGC TGGCAACAAC
TCCCAGCCCT GCTCCAATCC TGATCTCTTC GGCGAATTTC TCAATTCGGA CTCTGTGACC
GTCCCACCAT CCTTCCCGTC TGCCCACAGT GCTCCGCCCC CATCCTGCAG CGCCGACTTC
CTGCACCTGG GGGATCTGCC AGGAGAGCCC AGCAAGATGA CAGCCTCGTC CAGCAACCCA
GACCTGCTGG GAGGATGGGC TGCCTGGACC GAGACTGCAG CATCGGCAGT GGCCCCCACG
CCAGCCACAG AAGGCCCCCT CTTCTCTCCT GGAGGTCAGC CGGCCCCTTG TGGCTCTCAG
GCCAGCTGGA CCAAGTCTCA GAACCCGGAC CCATTTGCTG ACCTTGGCGA CCTCAGCTCC
GGCCTCCAAG GCTCACCAGC TGGATTCCCT CCTGGGGGCT TCATTCCCAA AACGGCCACC
ACGCCCAAAG GCAGCAGCTC CTGGCAGACA AGTCGGCCGC CAGCCCAGGG CGCCTCATGG
CCCCCTCAGG CCAAGCCGCC CCCCAAAGCC TGCACACAGC CAAGGCCTAA CTATGCCTCG
AACTTCAGTG TGATCGGGGC GCGGGAGGAG CGGGGGGTCC GCGCACCCAG CTTTGCTCAA
AAGCCAAAAG TCTCTGAGAA CGACTTTGAA GATCTGTTGT CCAATCAAGG CTTCTCCTCC
AGGTCTGACA AGAAAGGGCC AAAGACCATT GCAGAGATGA GGAAGCAGGA CCTGGCTAAA
GACACGGACC CACTCAAGCT GAAGCTCCTG GACTGGATTG AGGGCAAGGA GCGGAACATC
CGGGCCCTGC TGTCCACGCT GCACACAGTG CTGTGGGACG GGGAGAGCCG CTGGACGCCC
GTGGGCATGG CCGACCTGGT GGCTCCGGAG CAAGTGAAGA AGCACTATCG CCGCGCGGTG
CTGGCTGTGC ACCCCGACAA GGCTGCGGGG CAGCCGTACG AGCAGCACGC CAAGATGATC
TTCATGGAGC TGAATGACGC CTGGTCGGAG TTTGAGAACC AGGGCTCCCG GCCCCTCTTC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005272325.1
CDS144..4046
Misc Feature(1)258..1103
Misc Feature(2)264..1070
Misc Feature(3)order(279..293,303..305,342..344,348..350,438..440,510..521,528..530,534..536,660..662,666..668,672..677,681..683,714..716,723..725,807..818)
Misc Feature(4)order(279..293,303..305,342..344,348..350,438..440,510..521,528..530,660..662,666..668,672..677,681..683,714..716)
Misc Feature(5)order(291..293,528..530,534..536,660..662,666..668,672..674,723..725,807..818)
Misc Feature(6)order(711..803,807..818)
Misc Feature(7)1851..2267
Misc Feature(8)<3888..4022
Misc Feature(9)order(3933..3941,3975..3977,3984..3989,3996..4001)
Translation

Target ORF information:

RefSeq Version XM_005272268.2
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin G associated kinase (GAK), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005272268.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATGTG
GGGAGTGGCA GAGAGTATGC ATTAAAGAGG CTATTATCCA ATGAAGAGGA AAAGAACAGA
GCCATCATTC AAGAAGTTTG CTTCATGAAA AAGCTTTCCG GCCACCCGAA CATTGTCCAG
TTTTGTTCTG CAGCGTCTAT AGGAAAAGAG GAGTCAGACA CGGGGCAGGC TGAGTTCCTC
TTGCTCACAG AGCTCTGTAA AGGGCAGCTG GTGGAATTTT TGAAGAAAAT GGAATCTCGA
GGCCCCCTTT CGTGCGACAC GGTTCTGAAG ATCTTCTACC AGACGTGCCG CGCCGTGCAG
CACATGCACC GGCAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA GAACTTGTTG
CTTAGTAACC AAGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACGAC CATCTCGCAC
TACCCTGACT ACAGCTGGAG CGCCCAGAGG CGAGCCCTGG TGGAGGAAGA GATCACGAGG
AATACAACAC CAATGTATAG AACACCAGAA ATCATAGACT TGTATTCCAA CTTCCCGATC
GGCGAGAAGC AGGATATCTG GGCCCTGGGC TGCATCTTGT ACCTGCTGTG CTTCCGGCAG
CACCCTTTTG AGGATGGAGC GAAACTTCGA ATAGTCAATG GGAAGTACTC GATCCCCCCG
CACGACACGC AGTACACGGT CTTCCACAGC CTCATCCGCG CCATGCTGCA GGTGAACCCG
GAGGAGCGGC TGTCCATCGC CGAGGTGGTG CACCAGCTGC AGGAGATCGC GGCCGCCCGC
AACGTGAACC CCAAGTCTCC CATCACAGAG CTCCTGGAGC AGAATGGAGG CTACGGGAGC
GCCACACTGT CCCGAGGGCC ACCCCCTCCC GTGGGCCCCG CTGGCAGTGG CTACAGTGGA
GGCCTGGCGC TGGCGGAGTA CGACCAGCCG TATGGCGGCT TCCTGGACAT TCTGCGGGGT
GGGACAGAGC GGCTCTTCAC CAACCTCAAG GACACCTCCT CCAAGGTCAT CCAGTCCGTC
GCTAATTATG CAAAGGGTGA CCTGGACATA TCTTACATCA CATCCAGAAT TGCAGTGATG
TCATTCCCAG CAGAAGGTGT GGAGTCAGCG CTCAAAAACA ACATCGAAGA TGTGCGGTTG
TTCCTGGACT CCAAGCACCC AGGGCACTAT GCCGTCTACA ACCTGTCCCC GAGGACCTAC
CGGCCCTCCA GGTTCCACAA CCGGGTCTCC GAGTGTGGCT GGGCAGCACG GCGGGCCCCA
CACCTGCACA CCCTGTACAA CATCTGCAGG AACATGCACG CCTGGCTGCG GCAGGACCAC
AAGAACGTCT GCGTCGTGCA CTGCATGGAC GGGAGAGCCG CGTCTGCTGT GGCCGTCTGC
TCCTTCCTGT GCTTCTGCCG TCTCTTCAGC ACCGCGGAGG CCGCCGTGTA CATGTTCAGC
ATGAAGCGCT GCCCACCAGG CATCTGGCCA TCCCACAAAA GGTACATCGA GTACATGTGT
GACATGGTGG CGGAGGAGCC CATCACACCC CACAGCAAGC CCATCCTGGT GAGGGCCGTG
GTCATGACAC CCGTGCCGCT GTTCAGCAAG CAGAGGAGCG GCTGCAGGCC CTTCTGCGAG
GTCTACGTGG GGGACGAGCG TGTGGCCAGC ACCTCCCAGG AGTACGACAA GATGCGGGAC
TTTAAGATTG AAGATGGCAA AGCGGTGATT CCCCTGGGCG TCACGGTGCA AGGAGACGTG
CTCATCGTCA TCTATCACGC CCGGTCCACT CTGGGCGGCC GGCTGCAGGC CAAGATGGCA
TCCATGAAGA TGTTCCAGAT TCAGTTCCAC ACGGGGTTTG TGCCTCGGAA CGCCACCACT
GTGAAATTTG CCAAGTATGA CCTGGACGCG TGTGACATTC AAGAAAAATA CCCGGATTTA
TTTCAAGTGA ACCTGGAAGT GGAGGTGGAG CCCAGGGACA GGCCGAGCCG GGAAGCCCCA
CCATGGGAGA ACTCGAGCAT GAGGGGGCTG AACCCCAAAA TCCTGTTTTC CAGCCGGGAG
GAGCAGCAAG ACATTCTGTC TAAGTTTGGG AAGCCGGAGC TTCCCCGGCA GCCTGGCTCC
ACGGCTCAGT ATGATGCTGG GGCAGGGTCC CCGGAAGCCG AACCCACAGA CTCTGACTCA
CCGCCAAGCA GCAGCGCGGA CGCCAGTCGC TTCCTGCACA CGCTGGACTG GCAGGAAGAG
AAGGAGGCAG AGACTGGTGC AGAAAATGCC TCTTCCAAGG AGAGCGAGTC TGCCCTGATG
GAGGACAGAG ACGAGAGTGA GGTGTCAGAT GAAGGGGGAT CCCCGATCTC CAGCGAGGGC
CAGGAACCCA GGGCCGACCC AGAGCCCCCC GGCCTGGCAG CAGGGCTGGT GCAGCAGGAC
TTGGTTTTTG AGGTGGAGAC ACCGGCTGTG CTGCCAGAGC CTGTGCCACA GGAAGACGGG
GTCGACCTCC TGGGCCTGCA CTCCGAGGTG GGCGCAGGGC CAGCTGTACC CCCGCAGGCC
TGCAAGGCCC CCTCCAGCAA CACCGACCTG CTCAGCTGCC TCCTTGGGCC CCCTGAGGCC
GCCTCCCAGG GGCCCCCGGA GGATCTGCTC AGCGAGGACC CGCTGCTCCT GGCAAGCCCG
GCCCCTCCCC TGAGCGTGCA GAGCACCCCA AGAGGAGGGC CCCCTGCCGC TGGCAACAAC
TCCCAGCCCT GCTCCAATCC TGATCTCTTC GGCGAATTTC TCAATTCGGA CTCTGTGACC
GTCCCACCAT CCTTCCCGTC TGCCCACAGT GCTCCGCCCC CATCCTGCAG CGCCGACTTC
CTGCACCTGG GGGATCTGCC AGGAGAGCCC AGCAAGATGA CAGCCTCGTC CAGCAACCCA
GACCTGCTGG GAGGATGGGC TGCCTGGACC GAGACTGCAG CATCGGCAGT GGCCCCCACG
CCAGCCACAG AAGGCCCCCT CTTCTCTCCT GGAGGTCAGC CGGCCCCTTG TGGCTCTCAG
GCCAGCTGGA CCAAGTCTCA GAACCCGGAC CCATTTGCTG ACCTTGGCGA CCTCAGCTCC
GGCCTCCAAG GCTCACCAGC TGGATTCCCT CCTGGGGGCT TCATTCCCAA AACGGCCACC
ACGCCCAAAG GCAGCAGCTC CTGGCAGACA AGTCGGCCGC CAGCCCAGGG CGCCTCATGG
CCCCCTCAGG CCAAGCCGCC CCCCAAAGCC TGCACACAGC CAAGGCCTAA CTATGCCTCG
AACTTCAGTG TGATCGGGGC GCGGGAGGAG CGGGGGGTCC GCGCACCCAG CTTTGCTCAA
AAGCCAAAAG TCTCTGAGAA CGACTTTGAA GATCTGTTGT CCAATCAAGG CTTCTCCTCC
AGGTCTGACA AGAAAGGGCC AAAGACCATT GCAGAGATGA GGAAGCAGGA CCTGGCTAAA
GACACGGACC CACTCAAGCT GAAGCTCCTG GACTGGATTG AGGGCAAGGA GCGGAACATC
CGGGCCCTGC TGTCCACGCT GCACACAGTG CTGTGGGACG GGGAGAGCCG CTGGACGCCC
GTGGGCATGG CCGACCTGGT GGCTCCGGAG CAAGTGAAGA AGCACTATCG CCGCGCGGTG
CTGGCTGTGC ACCCCGACAA GGCTGCGGGG CAGCCGTACG AGCAGCACGC CAAGATGATC
TTCATGGAGC TGAATGACGC CTGGTCGGAG TTTGAGAACC AGGGCTCCCG GCCCCTCTTC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu38323
Clone ID Related Accession (Same CDS sequence) XM_005272270.2
Accession Version XM_005272270.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product cyclin-G-associated kinase isoform X12
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005272270.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATGTG
GGGAGTGGCA GAGAGTATGC ATTAAAGAGG CTATTATCCA ATGAAGAGGA AAAGAACAGA
GCCATCATTC AAGAAGTTTG CTTCATGAAA AAGCTTTCCG GCCACCCGAA CATTGTCCAG
TTTTGTTCTG CAGCGTCTAT AGGAAAAGAG GAGTCAGACA CGGGGCAGGC TGAGTTCCTC
TTGCTCACAG AGCTCTGTAA AGGGCAGCTG GTGGAATTTT TGAAGAAAAT GGAATCTCGA
GGCCCCCTTT CGTGCGACAC GGTTCTGAAG ATCTTCTACC AGACGTGCCG CGCCGTGCAG
CACATGCACC GGCAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA GAACTTGTTG
CTTAGTAACC AAGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACGAC CATCTCGCAC
TACCCTGACT ACAGCTGGAG CGCCCAGAGG CGAGCCCTGG TGGAGGAAGA GATCACGAGG
AATACAACAC CAATGTATAG AACACCAGAA ATCATAGACT TGTATTCCAA CTTCCCGATC
GGCGAGAAGC AGGATATCTG GGCCCTGGGC TGCATCTTGT ACCTGCTGTG CTTCCGGCAG
CACCCTTTTG AGGATGGAGC GAAACTTCGA ATAGTCAATG GGAAGTACTC GATCCCCCCG
CACGACACGC AGTACACGGT CTTCCACAGC CTCATCCGCG CCATGCTGCA GGTGAACCCG
GAGGAGCGGC TGTCCATCGC CGAGGTGGTG CACCAGCTGC AGGAGATCGC GGCCGCCCGC
AACGTGAACC CCAAGTCTCC CATCACAGAG CTCCTGGAGC AGAATGGAGG CTACGGGAGC
GCCACACTGT CCCGAGGGCC ACCCCCTCCC GTGGGCCCCG CTGGCAGTGG CTACAGTGGA
GGCCTGGCGC TGGCGGAGTA CGACCAGCCG TATGGCGGCT TCCTGGACAT TCTGCGGGGT
GGGACAGAGC GGCTCTTCAC CAACCTCAAG GACACCTCCT CCAAGGTCAT CCAGTCCGTC
GCTAATTATG CAAAGGGTGA CCTGGACATA TCTTACATCA CATCCAGAAT TGCAGTGATG
TCATTCCCAG CAGAAGGTGT GGAGTCAGCG CTCAAAAACA ACATCGAAGA TGTGCGGTTG
TTCCTGGACT CCAAGCACCC AGGGCACTAT GCCGTCTACA ACCTGTCCCC GAGGACCTAC
CGGCCCTCCA GGTTCCACAA CCGGGTCTCC GAGTGTGGCT GGGCAGCACG GCGGGCCCCA
CACCTGCACA CCCTGTACAA CATCTGCAGG AACATGCACG CCTGGCTGCG GCAGGACCAC
AAGAACGTCT GCGTCGTGCA CTGCATGGAC GGGAGAGCCG CGTCTGCTGT GGCCGTCTGC
TCCTTCCTGT GCTTCTGCCG TCTCTTCAGC ACCGCGGAGG CCGCCGTGTA CATGTTCAGC
ATGAAGCGCT GCCCACCAGG CATCTGGCCA TCCCACAAAA GGTACATCGA GTACATGTGT
GACATGGTGG CGGAGGAGCC CATCACACCC CACAGCAAGC CCATCCTGGT GAGGGCCGTG
GTCATGACAC CCGTGCCGCT GTTCAGCAAG CAGAGGAGCG GCTGCAGGCC CTTCTGCGAG
GTCTACGTGG GGGACGAGCG TGTGGCCAGC ACCTCCCAGG AGTACGACAA GATGCGGGAC
TTTAAGATTG AAGATGGCAA AGCGGTGATT CCCCTGGGCG TCACGGTGCA AGGAGACGTG
CTCATCGTCA TCTATCACGC CCGGTCCACT CTGGGCGGCC GGCTGCAGGC CAAGATGGCA
TCCATGAAGA TGTTCCAGAT TCAGTTCCAC ACGGGGTTTG TGCCTCGGAA CGCCACCACT
GTGAAATTTG CCAAGTATGA CCTGGACGCG TGTGACATTC AAGAAAAATA CCCGGATTTA
TTTCAAGTGA ACCTGGAAGT GGAGGTGGAG CCCAGGGACA GGCCGAGCCG GGAAGCCCCA
CCATGGGAGA ACTCGAGCAT GAGGGGGCTG AACCCCAAAA TCCTGTTTTC CAGCCGGGAG
GAGCAGCAAG ACATTCTGTC TAAGTTTGGG AAGCCGGAGC TTCCCCGGCA GCCTGGCTCC
ACGGCTCAGT ATGATGCTGG GGCAGGGTCC CCGGAAGCCG AACCCACAGA CTCTGACTCA
CCGCCAAGCA GCAGCGCGGA CGCCAGTCGC TTCCTGCACA CGCTGGACTG GCAGGAAGAG
AAGGAGGCAG AGACTGGTGC AGAAAATGCC TCTTCCAAGG AGAGCGAGTC TGCCCTGATG
GAGGACAGAG ACGAGAGTGA GGTGTCAGAT GAAGGGGGAT CCCCGATCTC CAGCGAGGGC
CAGGAACCCA GGGCCGACCC AGAGCCCCCC GGCCTGGCAG CAGGGCTGGT GCAGCAGGAC
TTGGTTTTTG AGGTGGAGAC ACCGGCTGTG CTGCCAGAGC CTGTGCCACA GGAAGACGGG
GTCGACCTCC TGGGCCTGCA CTCCGAGGTG GGCGCAGGGC CAGCTGTACC CCCGCAGGCC
TGCAAGGCCC CCTCCAGCAA CACCGACCTG CTCAGCTGCC TCCTTGGGCC CCCTGAGGCC
GCCTCCCAGG GGCCCCCGGA GGATCTGCTC AGCGAGGACC CGCTGCTCCT GGCAAGCCCG
GCCCCTCCCC TGAGCGTGCA GAGCACCCCA AGAGGAGGGC CCCCTGCCGC TGCTGACCCC
TTTGGCCCGC TTCTGCCGTC TTCAGGCAAC AACTCCCAGC CCTGCTCCAA TCCTGATCTC
TTCGGCGAAT TTCTCAATTC GGACTCTGTG ACCGTCCCAC CATCCTTCCC GTCTGCCCAC
AGTGCTCCGC CCCCATCCTG CAGCGCCGAC TTCCTGCACC TGGGGGATCT GCCAGGAGAG
CCCAGCAAGA TGACAGCCTC GTCCAGCAAC CCAGACCTGC TGGGAGGATG GGCTGCCTGG
ACCGAGACTG CAGCATCGGC AGTGGCCCCC ACGCCAGCCA CAGAAGGCCC CCTCTTCTCT
CCTGGAGGTC AGCCGGCCCC TTGTGGCTCT CAGGCCAGCT GGACCAAGTC TCAGAACCCG
GACCCATTTG CTGACCTTGG CGACCTCAGC TCCGGCCTCC AAGCTCAAAA GCCAAAAGTC
TCTGAGAACG ACTTTGAAGA TCTGTTGTCC AATCAAGGCT TCTCCTCCAG GTCTGACAAG
AAAGGGCCAA AGACCATTGC AGAGATGAGG AAGCAGGACC TGGCTAAAGA CACGGACCCA
CTCAAGCTGA AGCTCCTGGA CTGGATTGAG GGCAAGGAGC GGAACATCCG GGCCCTGCTG
TCCACGCTGC ACACAGTGCT GTGGGACGGG GAGAGCCGCT GGACGCCCGT GGGCATGGCC
GACCTGGTGG CTCCGGAGCA AGTGAAGAAG CACTATCGCC GCGCGGTGCT GGCTGTGCAC
CCCGACAAGG CTGCGGGGCA GCCGTACGAG CAGCACGCCA AGATGATCTT CATGGAGCTG
AATGACGCCT GGTCGGAGTT TGAGAACCAG GGCTCCCGGC CCCTCTTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005272327.1
CDS144..3854
Misc Feature(1)258..1103
Misc Feature(2)264..1070
Misc Feature(3)order(279..293,303..305,342..344,348..350,438..440,510..521,528..530,534..536,660..662,666..668,672..677,681..683,714..716,723..725,807..818)
Misc Feature(4)order(279..293,303..305,342..344,348..350,438..440,510..521,528..530,660..662,666..668,672..677,681..683,714..716)
Misc Feature(5)order(291..293,528..530,534..536,660..662,666..668,672..674,723..725,807..818)
Misc Feature(6)order(711..803,807..818)
Misc Feature(7)1851..2267
Misc Feature(8)<3696..3830
Misc Feature(9)order(3741..3749,3783..3785,3792..3797,3804..3809)
Translation

Target ORF information:

RefSeq Version XM_005272270.2
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin G associated kinase (GAK), transcript variant X12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005272270.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATGTG
GGGAGTGGCA GAGAGTATGC ATTAAAGAGG CTATTATCCA ATGAAGAGGA AAAGAACAGA
GCCATCATTC AAGAAGTTTG CTTCATGAAA AAGCTTTCCG GCCACCCGAA CATTGTCCAG
TTTTGTTCTG CAGCGTCTAT AGGAAAAGAG GAGTCAGACA CGGGGCAGGC TGAGTTCCTC
TTGCTCACAG AGCTCTGTAA AGGGCAGCTG GTGGAATTTT TGAAGAAAAT GGAATCTCGA
GGCCCCCTTT CGTGCGACAC GGTTCTGAAG ATCTTCTACC AGACGTGCCG CGCCGTGCAG
CACATGCACC GGCAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA GAACTTGTTG
CTTAGTAACC AAGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACGAC CATCTCGCAC
TACCCTGACT ACAGCTGGAG CGCCCAGAGG CGAGCCCTGG TGGAGGAAGA GATCACGAGG
AATACAACAC CAATGTATAG AACACCAGAA ATCATAGACT TGTATTCCAA CTTCCCGATC
GGCGAGAAGC AGGATATCTG GGCCCTGGGC TGCATCTTGT ACCTGCTGTG CTTCCGGCAG
CACCCTTTTG AGGATGGAGC GAAACTTCGA ATAGTCAATG GGAAGTACTC GATCCCCCCG
CACGACACGC AGTACACGGT CTTCCACAGC CTCATCCGCG CCATGCTGCA GGTGAACCCG
GAGGAGCGGC TGTCCATCGC CGAGGTGGTG CACCAGCTGC AGGAGATCGC GGCCGCCCGC
AACGTGAACC CCAAGTCTCC CATCACAGAG CTCCTGGAGC AGAATGGAGG CTACGGGAGC
GCCACACTGT CCCGAGGGCC ACCCCCTCCC GTGGGCCCCG CTGGCAGTGG CTACAGTGGA
GGCCTGGCGC TGGCGGAGTA CGACCAGCCG TATGGCGGCT TCCTGGACAT TCTGCGGGGT
GGGACAGAGC GGCTCTTCAC CAACCTCAAG GACACCTCCT CCAAGGTCAT CCAGTCCGTC
GCTAATTATG CAAAGGGTGA CCTGGACATA TCTTACATCA CATCCAGAAT TGCAGTGATG
TCATTCCCAG CAGAAGGTGT GGAGTCAGCG CTCAAAAACA ACATCGAAGA TGTGCGGTTG
TTCCTGGACT CCAAGCACCC AGGGCACTAT GCCGTCTACA ACCTGTCCCC GAGGACCTAC
CGGCCCTCCA GGTTCCACAA CCGGGTCTCC GAGTGTGGCT GGGCAGCACG GCGGGCCCCA
CACCTGCACA CCCTGTACAA CATCTGCAGG AACATGCACG CCTGGCTGCG GCAGGACCAC
AAGAACGTCT GCGTCGTGCA CTGCATGGAC GGGAGAGCCG CGTCTGCTGT GGCCGTCTGC
TCCTTCCTGT GCTTCTGCCG TCTCTTCAGC ACCGCGGAGG CCGCCGTGTA CATGTTCAGC
ATGAAGCGCT GCCCACCAGG CATCTGGCCA TCCCACAAAA GGTACATCGA GTACATGTGT
GACATGGTGG CGGAGGAGCC CATCACACCC CACAGCAAGC CCATCCTGGT GAGGGCCGTG
GTCATGACAC CCGTGCCGCT GTTCAGCAAG CAGAGGAGCG GCTGCAGGCC CTTCTGCGAG
GTCTACGTGG GGGACGAGCG TGTGGCCAGC ACCTCCCAGG AGTACGACAA GATGCGGGAC
TTTAAGATTG AAGATGGCAA AGCGGTGATT CCCCTGGGCG TCACGGTGCA AGGAGACGTG
CTCATCGTCA TCTATCACGC CCGGTCCACT CTGGGCGGCC GGCTGCAGGC CAAGATGGCA
TCCATGAAGA TGTTCCAGAT TCAGTTCCAC ACGGGGTTTG TGCCTCGGAA CGCCACCACT
GTGAAATTTG CCAAGTATGA CCTGGACGCG TGTGACATTC AAGAAAAATA CCCGGATTTA
TTTCAAGTGA ACCTGGAAGT GGAGGTGGAG CCCAGGGACA GGCCGAGCCG GGAAGCCCCA
CCATGGGAGA ACTCGAGCAT GAGGGGGCTG AACCCCAAAA TCCTGTTTTC CAGCCGGGAG
GAGCAGCAAG ACATTCTGTC TAAGTTTGGG AAGCCGGAGC TTCCCCGGCA GCCTGGCTCC
ACGGCTCAGT ATGATGCTGG GGCAGGGTCC CCGGAAGCCG AACCCACAGA CTCTGACTCA
CCGCCAAGCA GCAGCGCGGA CGCCAGTCGC TTCCTGCACA CGCTGGACTG GCAGGAAGAG
AAGGAGGCAG AGACTGGTGC AGAAAATGCC TCTTCCAAGG AGAGCGAGTC TGCCCTGATG
GAGGACAGAG ACGAGAGTGA GGTGTCAGAT GAAGGGGGAT CCCCGATCTC CAGCGAGGGC
CAGGAACCCA GGGCCGACCC AGAGCCCCCC GGCCTGGCAG CAGGGCTGGT GCAGCAGGAC
TTGGTTTTTG AGGTGGAGAC ACCGGCTGTG CTGCCAGAGC CTGTGCCACA GGAAGACGGG
GTCGACCTCC TGGGCCTGCA CTCCGAGGTG GGCGCAGGGC CAGCTGTACC CCCGCAGGCC
TGCAAGGCCC CCTCCAGCAA CACCGACCTG CTCAGCTGCC TCCTTGGGCC CCCTGAGGCC
GCCTCCCAGG GGCCCCCGGA GGATCTGCTC AGCGAGGACC CGCTGCTCCT GGCAAGCCCG
GCCCCTCCCC TGAGCGTGCA GAGCACCCCA AGAGGAGGGC CCCCTGCCGC TGCTGACCCC
TTTGGCCCGC TTCTGCCGTC TTCAGGCAAC AACTCCCAGC CCTGCTCCAA TCCTGATCTC
TTCGGCGAAT TTCTCAATTC GGACTCTGTG ACCGTCCCAC CATCCTTCCC GTCTGCCCAC
AGTGCTCCGC CCCCATCCTG CAGCGCCGAC TTCCTGCACC TGGGGGATCT GCCAGGAGAG
CCCAGCAAGA TGACAGCCTC GTCCAGCAAC CCAGACCTGC TGGGAGGATG GGCTGCCTGG
ACCGAGACTG CAGCATCGGC AGTGGCCCCC ACGCCAGCCA CAGAAGGCCC CCTCTTCTCT
CCTGGAGGTC AGCCGGCCCC TTGTGGCTCT CAGGCCAGCT GGACCAAGTC TCAGAACCCG
GACCCATTTG CTGACCTTGG CGACCTCAGC TCCGGCCTCC AAGCTCAAAA GCCAAAAGTC
TCTGAGAACG ACTTTGAAGA TCTGTTGTCC AATCAAGGCT TCTCCTCCAG GTCTGACAAG
AAAGGGCCAA AGACCATTGC AGAGATGAGG AAGCAGGACC TGGCTAAAGA CACGGACCCA
CTCAAGCTGA AGCTCCTGGA CTGGATTGAG GGCAAGGAGC GGAACATCCG GGCCCTGCTG
TCCACGCTGC ACACAGTGCT GTGGGACGGG GAGAGCCGCT GGACGCCCGT GGGCATGGCC
GACCTGGTGG CTCCGGAGCA AGTGAAGAAG CACTATCGCC GCGCGGTGCT GGCTGTGCAC
CCCGACAAGG CTGCGGGGCA GCCGTACGAG CAGCACGCCA AGATGATCTT CATGGAGCTG
AATGACGCCT GGTCGGAGTT TGAGAACCAG GGCTCCCGGC CCCTCTTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57905
Clone ID Related Accession (Same CDS sequence) XM_011513427.2
Accession Version XM_011513427.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3978bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product cyclin-G-associated kinase isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011513427.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATGTG
GGGAGTGGCA GAGAGTATGC ATTAAAGAGG CTATTATCCA ATGAAGAGGA AAAGAACAGA
GCCATCATTC AAGAAGTTTG CTTCATGAAA AAGCTTTCCG GCCACCCGAA CATTGTCCAG
TTTTGTTCTG CAGCGTCTAT AGGAAAAGAG GAGTCAGACA CGGGGCAGGC TGAGTTCCTC
TTGCTCACAG AGCTCTGTAA AGGGCAGCTG GTGGAATTTT TGAAGAAAAT GGAATCTCGA
GGCCCCCTTT CGTGCGACAC GGTTCTGAAG ATCTTCTACC AGACGTGCCG CGCCGTGCAG
CACATGCACC GGCAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA GAACTTGTTG
CTTAGTAACC AAGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACGAC CATCTCGCAC
TACCCTGACT ACAGCTGGAG CGCCCAGAGG CGAGCCCTGG TGGAGGAAGA GGCCCTGGGC
TGCATCTTGT ACCTGCTGTG CTTCCGGCAG CACCCTTTTG AGGATGGAGC GAAACTTCGA
ATAGTCAATG GGAAGTACTC GATCCCCCCG CACGACACGC AGTACACGGT CTTCCACAGC
CTCATCCGCG CCATGCTGCA GGTGAACCCG GAGGAGCGGC TGTCCATCGC CGAGGTGGTG
CACCAGCTGC AGGAGATCGC GGCCGCCCGC AACGTGAACC CCAAGTCTCC CATCACAGAG
CTCCTGGAGC AGAATGGAGG CTACGGGAGC GCCACACTGT CCCGAGGGCC ACCCCCTCCC
GTGGGCCCCG CTGGCAGTGG CTACAGTGGA GGCCTGGCGC TGGCGGAGTA CGACCAGCCG
TATGGCGGCT TCCTGGACAT TCTGCGGGGT GGGACAGAGC GGCTCTTCAC CAACCTCAAG
GACACCTCCT CCAAGGTCAT CCAGTCCGTC GCTAATTATG CAAAGGGTGA CCTGGACATA
TCTTACATCA CATCCAGAAT TGCAGTGATG TCATTCCCAG CAGAAGGTGT GGAGTCAGCG
CTCAAAAACA ACATCGAAGA TGTGCGGTTG TTCCTGGACT CCAAGCACCC AGGGCACTAT
GCCGTCTACA ACCTGTCCCC GAGGACCTAC CGGCCCTCCA GGTTCCACAA CCGGGTCTCC
GAGTGTGGCT GGGCAGCACG GCGGGCCCCA CACCTGCACA CCCTGTACAA CATCTGCAGG
AACATGCACG CCTGGCTGCG GCAGGACCAC AAGAACGTCT GCGTCGTGCA CTGCATGGAC
GGGAGAGCCG CGTCTGCTGT GGCCGTCTGC TCCTTCCTGT GCTTCTGCCG TCTCTTCAGC
ACCGCGGAGG CCGCCGTGTA CATGTTCAGC ATGAAGCGCT GCCCACCAGG CATCTGGCCA
TCCCACAAAA GGTACATCGA GTACATGTGT GACATGGTGG CGGAGGAGCC CATCACACCC
CACAGCAAGC CCATCCTGGT GAGGGCCGTG GTCATGACAC CCGTGCCGCT GTTCAGCAAG
CAGAGGAGCG GCTGCAGGCC CTTCTGCGAG GTCTACGTGG GGGACGAGCG TGTGGCCAGC
ACCTCCCAGG AGTACGACAA GATGCGGGAC TTTAAGATTG AAGATGGCAA AGCGGTGATT
CCCCTGGGCG TCACGGTGCA AGGAGACGTG CTCATCGTCA TCTATCACGC CCGGTCCACT
CTGGGCGGCC GGCTGCAGGC CAAGATGGCA TCCATGAAGA TGTTCCAGAT TCAGTTCCAC
ACGGGGTTTG TGCCTCGGAA CGCCACCACT GTGAAATTTG CCAAGTATGA CCTGGACGCG
TGTGACATTC AAGAAAAATA CCCGGATTTA TTTCAAGTGA ACCTGGAAGT GGAGGTGGAG
CCCAGGGACA GGCCGAGCCG GGAAGCCCCA CCATGGGAGA ACTCGAGCAT GAGGGGGCTG
AACCCCAAAA TCCTGTTTTC CAGCCGGGAG GAGCAGCAAG ACATTCTGTC TAAGTTTGGG
AAGCCGGAGC TTCCCCGGCA GCCTGGCTCC ACGGCTCAGT ATGATGCTGG GGCAGGGTCC
CCGGAAGCCG AACCCACAGA CTCTGACTCA CCGCCAAGCA GCAGCGCGGA CGCCAGTCGC
TTCCTGCACA CGCTGGACTG GCAGGAAGAG AAGGAGGCAG AGACTGGTGC AGAAAATGCC
TCTTCCAAGG AGAGCGAGTC TGCCCTGATG GAGGACAGAG ACGAGAGTGA GGTGTCAGAT
GAAGGGGGAT CCCCGATCTC CAGCGAGGGC CAGGAACCCA GGGCCGACCC AGAGCCCCCC
GGCCTGGCAG CAGGGCTGGT GCAGCAGGAC TTGGTTTTTG AGGTGGAGAC ACCGGCTGTG
CTGCCAGAGC CTGTGCCACA GGAAGACGGG GTCGACCTCC TGGGCCTGCA CTCCGAGGTG
GGCGCAGGGC CAGCTGTACC CCCGCAGGCC TGCAAGGCCC CCTCCAGCAA CACCGACCTG
CTCAGCTGCC TCCTTGGGCC CCCTGAGGCC GCCTCCCAGG GGCCCCCGGA GGATCTGCTC
AGCGAGGACC CGCTGCTCCT GGCAAGCCCG GCCCCTCCCC TGAGCGTGCA GAGCACCCCA
AGAGGAGGGC CCCCTGCCGC TGCTGACCCC TTTGGCCCGC TTCTGCCGTC TTCAGGCAAC
AACTCCCAGC CCTGCTCCAA TCCTGATCTC TTCGGCGAAT TTCTCAATTC GGACTCTGTG
ACCGTCCCAC CATCCTTCCC GTCTGCCCAC AGTGCTCCGC CCCCATCCTG CAGCGCCGAC
TTCCTGCACC TGGGGGATCT GCCAGGAGAG CCCAGCAAGA TGACAGCCTC GTCCAGCAAC
CCAGACCTGC TGGGAGGATG GGCTGCCTGG ACCGAGACTG CAGCATCGGC AGTGGCCCCC
ACGCCAGCCA CAGAAGGCCC CCTCTTCTCT CCTGGAGGTC AGCCGGCCCC TTGTGGCTCT
CAGGCCAGCT GGACCAAGTC TCAGAACCCG GACCCATTTG CTGACCTTGG CGACCTCAGC
TCCGGCCTCC AAGACCCCCA AGCCCAGAGC ACAGTGAGCC CAAGGGGACA GCGTGTCTGC
ACCTGTTCCA GGCGACTGCC AACTGGCAAG CTAAAACCGG GAGTTGCTGA CACTGGCACT
GCTGCCAGCC CCCACCGGCA TTGTGGCTCA CCAGCTGGAT TCCCTCCTGG GGGCTTCATT
CCCAAAACGG CCACCACGCC CAAAGGCAGC AGCTCCTGGC AGACAAGTCG GCCGCCAGCC
CAGGGCGCCT CATGGCCCCC TCAGGCCAAG CCGCCCCCCA AAGCCTGCAC ACAGCCAAGG
CCTAACTATG CCTCGAACTT CAGTGTGATC GGGGCGCGGG AGGAGCGGGG GGTCCGCGCA
CCCAGCTTTG CTCAAAAGCC AAAAGTCTCT GAGAACGACT TTGAAGATCT GTTGTCCAAT
CAAGGCTTCT CCTCCAGGTC TGACAAGAAA GGGCCAAAGA CCATTGCAGA GATGAGGAAG
CAGGACCTGG CTAAAGACAC GGACCCACTC AAGCTGAAGC TCCTGGACTG GATTGAGGGC
AAGGAGCGGA ACATCCGGGC CCTGCTGTCC ACGCTGCACA CAGTGCTGTG GGACGGGGAG
AGCCGCTGGA CGCCCGTGGG CATGGCCGAC CTGGTGGCTC CGGAGCAAGT GAAGAAGCAC
TATCGCCGCG CGGTGCTGGC TGTGCACCCC GACAAGGCTG CGGGGCAGCC GTACGAGCAG
CACGCCAAGA TGATCTTCAT GGAGCTGAAT GACGCCTGGT CGGAGTTTGA GAACCAGGGC
TCCCGGCCCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011511729.1
CDS144..4121
Misc Feature(1)258..1013
Misc Feature(2)264..980
Misc Feature(3)order(279..290,297..299,303..305,342..344,348..350,438..440,510..521,660..662,672..677,681..683,711..716)
Misc Feature(4)1761..2177
Misc Feature(5)<3963..4097
Misc Feature(6)order(4008..4016,4050..4052,4059..4064,4071..4076)
Translation

Target ORF information:

RefSeq Version XM_011513427.2
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin G associated kinase (GAK), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011513427.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATGTG
GGGAGTGGCA GAGAGTATGC ATTAAAGAGG CTATTATCCA ATGAAGAGGA AAAGAACAGA
GCCATCATTC AAGAAGTTTG CTTCATGAAA AAGCTTTCCG GCCACCCGAA CATTGTCCAG
TTTTGTTCTG CAGCGTCTAT AGGAAAAGAG GAGTCAGACA CGGGGCAGGC TGAGTTCCTC
TTGCTCACAG AGCTCTGTAA AGGGCAGCTG GTGGAATTTT TGAAGAAAAT GGAATCTCGA
GGCCCCCTTT CGTGCGACAC GGTTCTGAAG ATCTTCTACC AGACGTGCCG CGCCGTGCAG
CACATGCACC GGCAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA GAACTTGTTG
CTTAGTAACC AAGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACGAC CATCTCGCAC
TACCCTGACT ACAGCTGGAG CGCCCAGAGG CGAGCCCTGG TGGAGGAAGA GGCCCTGGGC
TGCATCTTGT ACCTGCTGTG CTTCCGGCAG CACCCTTTTG AGGATGGAGC GAAACTTCGA
ATAGTCAATG GGAAGTACTC GATCCCCCCG CACGACACGC AGTACACGGT CTTCCACAGC
CTCATCCGCG CCATGCTGCA GGTGAACCCG GAGGAGCGGC TGTCCATCGC CGAGGTGGTG
CACCAGCTGC AGGAGATCGC GGCCGCCCGC AACGTGAACC CCAAGTCTCC CATCACAGAG
CTCCTGGAGC AGAATGGAGG CTACGGGAGC GCCACACTGT CCCGAGGGCC ACCCCCTCCC
GTGGGCCCCG CTGGCAGTGG CTACAGTGGA GGCCTGGCGC TGGCGGAGTA CGACCAGCCG
TATGGCGGCT TCCTGGACAT TCTGCGGGGT GGGACAGAGC GGCTCTTCAC CAACCTCAAG
GACACCTCCT CCAAGGTCAT CCAGTCCGTC GCTAATTATG CAAAGGGTGA CCTGGACATA
TCTTACATCA CATCCAGAAT TGCAGTGATG TCATTCCCAG CAGAAGGTGT GGAGTCAGCG
CTCAAAAACA ACATCGAAGA TGTGCGGTTG TTCCTGGACT CCAAGCACCC AGGGCACTAT
GCCGTCTACA ACCTGTCCCC GAGGACCTAC CGGCCCTCCA GGTTCCACAA CCGGGTCTCC
GAGTGTGGCT GGGCAGCACG GCGGGCCCCA CACCTGCACA CCCTGTACAA CATCTGCAGG
AACATGCACG CCTGGCTGCG GCAGGACCAC AAGAACGTCT GCGTCGTGCA CTGCATGGAC
GGGAGAGCCG CGTCTGCTGT GGCCGTCTGC TCCTTCCTGT GCTTCTGCCG TCTCTTCAGC
ACCGCGGAGG CCGCCGTGTA CATGTTCAGC ATGAAGCGCT GCCCACCAGG CATCTGGCCA
TCCCACAAAA GGTACATCGA GTACATGTGT GACATGGTGG CGGAGGAGCC CATCACACCC
CACAGCAAGC CCATCCTGGT GAGGGCCGTG GTCATGACAC CCGTGCCGCT GTTCAGCAAG
CAGAGGAGCG GCTGCAGGCC CTTCTGCGAG GTCTACGTGG GGGACGAGCG TGTGGCCAGC
ACCTCCCAGG AGTACGACAA GATGCGGGAC TTTAAGATTG AAGATGGCAA AGCGGTGATT
CCCCTGGGCG TCACGGTGCA AGGAGACGTG CTCATCGTCA TCTATCACGC CCGGTCCACT
CTGGGCGGCC GGCTGCAGGC CAAGATGGCA TCCATGAAGA TGTTCCAGAT TCAGTTCCAC
ACGGGGTTTG TGCCTCGGAA CGCCACCACT GTGAAATTTG CCAAGTATGA CCTGGACGCG
TGTGACATTC AAGAAAAATA CCCGGATTTA TTTCAAGTGA ACCTGGAAGT GGAGGTGGAG
CCCAGGGACA GGCCGAGCCG GGAAGCCCCA CCATGGGAGA ACTCGAGCAT GAGGGGGCTG
AACCCCAAAA TCCTGTTTTC CAGCCGGGAG GAGCAGCAAG ACATTCTGTC TAAGTTTGGG
AAGCCGGAGC TTCCCCGGCA GCCTGGCTCC ACGGCTCAGT ATGATGCTGG GGCAGGGTCC
CCGGAAGCCG AACCCACAGA CTCTGACTCA CCGCCAAGCA GCAGCGCGGA CGCCAGTCGC
TTCCTGCACA CGCTGGACTG GCAGGAAGAG AAGGAGGCAG AGACTGGTGC AGAAAATGCC
TCTTCCAAGG AGAGCGAGTC TGCCCTGATG GAGGACAGAG ACGAGAGTGA GGTGTCAGAT
GAAGGGGGAT CCCCGATCTC CAGCGAGGGC CAGGAACCCA GGGCCGACCC AGAGCCCCCC
GGCCTGGCAG CAGGGCTGGT GCAGCAGGAC TTGGTTTTTG AGGTGGAGAC ACCGGCTGTG
CTGCCAGAGC CTGTGCCACA GGAAGACGGG GTCGACCTCC TGGGCCTGCA CTCCGAGGTG
GGCGCAGGGC CAGCTGTACC CCCGCAGGCC TGCAAGGCCC CCTCCAGCAA CACCGACCTG
CTCAGCTGCC TCCTTGGGCC CCCTGAGGCC GCCTCCCAGG GGCCCCCGGA GGATCTGCTC
AGCGAGGACC CGCTGCTCCT GGCAAGCCCG GCCCCTCCCC TGAGCGTGCA GAGCACCCCA
AGAGGAGGGC CCCCTGCCGC TGCTGACCCC TTTGGCCCGC TTCTGCCGTC TTCAGGCAAC
AACTCCCAGC CCTGCTCCAA TCCTGATCTC TTCGGCGAAT TTCTCAATTC GGACTCTGTG
ACCGTCCCAC CATCCTTCCC GTCTGCCCAC AGTGCTCCGC CCCCATCCTG CAGCGCCGAC
TTCCTGCACC TGGGGGATCT GCCAGGAGAG CCCAGCAAGA TGACAGCCTC GTCCAGCAAC
CCAGACCTGC TGGGAGGATG GGCTGCCTGG ACCGAGACTG CAGCATCGGC AGTGGCCCCC
ACGCCAGCCA CAGAAGGCCC CCTCTTCTCT CCTGGAGGTC AGCCGGCCCC TTGTGGCTCT
CAGGCCAGCT GGACCAAGTC TCAGAACCCG GACCCATTTG CTGACCTTGG CGACCTCAGC
TCCGGCCTCC AAGACCCCCA AGCCCAGAGC ACAGTGAGCC CAAGGGGACA GCGTGTCTGC
ACCTGTTCCA GGCGACTGCC AACTGGCAAG CTAAAACCGG GAGTTGCTGA CACTGGCACT
GCTGCCAGCC CCCACCGGCA TTGTGGCTCA CCAGCTGGAT TCCCTCCTGG GGGCTTCATT
CCCAAAACGG CCACCACGCC CAAAGGCAGC AGCTCCTGGC AGACAAGTCG GCCGCCAGCC
CAGGGCGCCT CATGGCCCCC TCAGGCCAAG CCGCCCCCCA AAGCCTGCAC ACAGCCAAGG
CCTAACTATG CCTCGAACTT CAGTGTGATC GGGGCGCGGG AGGAGCGGGG GGTCCGCGCA
CCCAGCTTTG CTCAAAAGCC AAAAGTCTCT GAGAACGACT TTGAAGATCT GTTGTCCAAT
CAAGGCTTCT CCTCCAGGTC TGACAAGAAA GGGCCAAAGA CCATTGCAGA GATGAGGAAG
CAGGACCTGG CTAAAGACAC GGACCCACTC AAGCTGAAGC TCCTGGACTG GATTGAGGGC
AAGGAGCGGA ACATCCGGGC CCTGCTGTCC ACGCTGCACA CAGTGCTGTG GGACGGGGAG
AGCCGCTGGA CGCCCGTGGG CATGGCCGAC CTGGTGGCTC CGGAGCAAGT GAAGAAGCAC
TATCGCCGCG CGGTGCTGGC TGTGCACCCC GACAAGGCTG CGGGGCAGCC GTACGAGCAG
CACGCCAAGA TGATCTTCAT GGAGCTGAAT GACGCCTGGT CGGAGTTTGA GAACCAGGGC
TCCCGGCCCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57906
Clone ID Related Accession (Same CDS sequence) XM_011513428.2
Accession Version XM_011513428.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3921bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product cyclin-G-associated kinase isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011513428.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATGTG
GGGAGTGGCA GAGAGTATGC ATTAAAGAGG CTATTATCCA ATGAAGAGGA AAAGAACAGA
GCCATCATTC AAGAAGTTTG CTTCATGAAA AAGCTTTCCG GCCACCCGAA CATTGTCCAG
TTTTGTTCTG CAGCGTCTAT AGGAAAAGAG GAGTCAGACA CGGGGCAGGC TGAGTTCCTC
TTGCTCACAG AGCTCTGTAA AGGGCAGCTG GTGGAATTTT TGAAGAAAAT GGAATCTCGA
GGCCCCCTTT CGTGCGACAC GGTTCTGAAG ATCTTCTACC AGACGTGCCG CGCCGTGCAG
CACATGCACC GGCAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA GAACTTGTTG
CTTAGTAACC AAGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACGAC CATCTCGCAC
TACCCTGACT ACAGCTGGAG CGCCCAGAGG CGAGCCCTGG TGGAGGAAGA GATCACGAGG
AATACAACAC CAATGTATAG AACACCAGAA ATCATAGACT TGTATTCCAA CTTCCCGATC
GGCGAGAAGC AGGATATCTG GGCCCTGGGC TGCATCTTGT ACCTGCTGTG CTTCCGGCAG
CACCCTTTTG AGGATGGAGC GAAACTTCGA ATAGTCAATG GGAAGTACTC GATCCCCCCG
CACGACACGC AGTACACGGT CTTCCACAGC CTCATCCGCG CCATGCTGCA GGTGAACCCG
GAGGAGCGGC TGTCCATCGC CGAGGTGGTG CACCAGCTGC AGGAGATCGC GGCCGCCCGC
AACGTGAACC CCAAGTCTCC CATCACAGAG CTCCTGGAGC AGAATGGAGG CTACGGGAGC
GCCACACTGT CCCGAGGGCC ACCCCCTCCC GTGGGCCCCG CTGGCAGTGG CTACAGTGGA
GGCCTGGCGC TGGCGGAGTA CGACCAGCCG TATGGCGGCT TCCTGGACAT TCTGCGGGGT
GGGACAGAGC GGCTCTTCAC CAACCTCAAG GACACCTCCT CCAAGGTCAT CCAGTCCGTC
GCTAATTATG CAAAGGGTGA CCTGGACATA TCTTACATCA CATCCAGAAT TGCAGTGATG
TCATTCCCAG CAGAAGGTGT GGAGTCAGCG CTCAAAAACA ACATCGAAGA TGTGCGGTTG
TTCCTGGACT CCAAGCACCC AGGGCACTAT GCCGTCTACA ACCTGTCCCC GAGGACCTAC
CGGCCCTCCA GGTTCCACAA CCGGGTCTCC GAGTGTGGCT GGGCAGCACG GCGGGCCCCA
CACCTGCACA CCCTGTACAA CATCTGCAGG AACATGCACG CCTGGCTGCG GCAGGACCAC
AAGAACGTCT GCGTCGTGCA CTGCATGGAC GGGAGAGCCG CGTCTGCTGT GGCCGTCTGC
TCCTTCCTGT GCTTCTGCCG TCTCTTCAGC ACCGCGGAGG CCGCCGTGTA CATGTTCAGC
ATGAAGCGCT GCCCACCAGG CATCTGGCCA TCCCACAAAA GGTACATCGA GTACATGTGT
GACATGGTGG CGGAGGAGCC CATCACACCC CACAGCAAGC CCATCCTGGT GAGGGCCGTG
GTCATGACAC CCGTGCCGCT GTTCAGCAAG CAGAGGAGCG GCTGCAGGCC CTTCTGCGAG
GTCTACGTGG GGGACGAGCG TGTGGCCAGC ACCTCCCAGG AGTACGACAA GATGCGGGAC
TTTAAGATTG AAGATGGCAA AGCGGTGATT CCCCTGGGCG TCACGGTGCA AGGAGACGTG
CTCATCGTCA TCTATCACGC CCGGTCCACT CTGGGCGGCC GGCTGCAGGC CAAGATGGCA
TCCATGAAGA TGTTCCAGAT TCAGTTCCAC ACGGGGTTTG TGCCTCGGAA CGCCACCACT
GTGAAATTTG CCAAGTATGA CCTGGACGCG TGTGACATTC AAGAAAAATA CCCGGATTTA
TTTCAAGTGA ACCTGGAAGT GGAGGTGGAG CCCAGGGACA GGCCGAGCCG GGAAGCCCCA
CCATGGGAGA ACTCGAGCAT GAGGGGGCTG AACCCCAAAA TCCTGTTTTC CAGCCGGGAG
GAGCAGCAAG ACATTCTGTC TAAGTTTGAA GAGAAGGAGG CAGAGACTGG TGCAGAAAAT
GCCTCTTCCA AGGAGAGCGA GTCTGCCCTG ATGGAGGACA GAGACGAGAG TGAGGTGTCA
GATGAAGGGG GATCCCCGAT CTCCAGCGAG GGCCAGGAAC CCAGGGCCGA CCCAGAGCCC
CCCGGCCTGG CAGCAGGGCT GGTGCAGCAG GACTTGGTTT TTGAGGTGGA GACACCGGCT
GTGCTGCCAG AGCCTGTGCC ACAGGAAGAC GGGGTCGACC TCCTGGGCCT GCACTCCGAG
GTGGGCGCAG GGCCAGCTGT ACCCCCGCAG GCCTGCAAGG CCCCCTCCAG CAACACCGAC
CTGCTCAGCT GCCTCCTTGG GCCCCCTGAG GCCGCCTCCC AGGGGCCCCC GGAGGATCTG
CTCAGCGAGG ACCCGCTGCT CCTGGCAAGC CCGGCCCCTC CCCTGAGCGT GCAGAGCACC
CCAAGAGGAG GGCCCCCTGC CGCTGCTGAC CCCTTTGGCC CGCTTCTGCC GTCTTCAGGC
AACAACTCCC AGCCCTGCTC CAATCCTGAT CTCTTCGGCG AATTTCTCAA TTCGGACTCT
GTGACCGTCC CACCATCCTT CCCGTCTGCC CACAGTGCTC CGCCCCCATC CTGCAGCGCC
GACTTCCTGC ACCTGGGGGA TCTGCCAGGA GAGCCCAGCA AGATGACAGC CTCGTCCAGC
AACCCAGACC TGCTGGGAGG ATGGGCTGCC TGGACCGAGA CTGCAGCATC GGCAGTGGCC
CCCACGCCAG CCACAGAAGG CCCCCTCTTC TCTCCTGGAG GTCAGCCGGC CCCTTGTGGC
TCTCAGGCCA GCTGGACCAA GTCTCAGAAC CCGGACCCAT TTGCTGACCT TGGCGACCTC
AGCTCCGGCC TCCAAGACCC CCAAGCCCAG AGCACAGTGA GCCCAAGGGG ACAGCGTGTC
TGCACCTGTT CCAGGCGACT GCCAACTGGC AAGCTAAAAC CGGGAGTTGC TGACACTGGC
ACTGCTGCCA GCCCCCACCG GCATTGTGGC TCACCAGCTG GATTCCCTCC TGGGGGCTTC
ATTCCCAAAA CGGCCACCAC GCCCAAAGGC AGCAGCTCCT GGCAGACAAG TCGGCCGCCA
GCCCAGGGCG CCTCATGGCC CCCTCAGGCC AAGCCGCCCC CCAAAGCCTG CACACAGCCA
AGGCCTAACT ATGCCTCGAA CTTCAGTGTG ATCGGGGCGC GGGAGGAGCG GGGGGTCCGC
GCACCCAGCT TTGCTCAAAA GCCAAAAGTC TCTGAGAACG ACTTTGAAGA TCTGTTGTCC
AATCAAGGCT TCTCCTCCAG GTCTGACAAG AAAGGGCCAA AGACCATTGC AGAGATGAGG
AAGCAGGACC TGGCTAAAGA CACGGACCCA CTCAAGCTGA AGCTCCTGGA CTGGATTGAG
GGCAAGGAGC GGAACATCCG GGCCCTGCTG TCCACGCTGC ACACAGTGCT GTGGGACGGG
GAGAGCCGCT GGACGCCCGT GGGCATGGCC GACCTGGTGG CTCCGGAGCA AGTGAAGAAG
CACTATCGCC GCGCGGTGCT GGCTGTGCAC CCCGACAAGG CTGCGGGGCA GCCGTACGAG
CAGCACGCCA AGATGATCTT CATGGAGCTG AATGACGCCT GGTCGGAGTT TGAGAACCAG
GGCTCCCGGC CCCTCTTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011511730.1
CDS144..4064
Misc Feature(1)258..1103
Misc Feature(2)264..1070
Misc Feature(3)order(279..293,303..305,342..344,348..350,438..440,510..521,528..530,534..536,660..662,666..668,672..677,681..683,714..716,723..725,807..818)
Misc Feature(4)order(279..293,303..305,342..344,348..350,438..440,510..521,528..530,660..662,666..668,672..677,681..683,714..716)
Misc Feature(5)order(291..293,528..530,534..536,660..662,666..668,672..674,723..725,807..818)
Misc Feature(6)order(711..803,807..818)
Misc Feature(7)1851..2267
Misc Feature(8)<3906..4040
Misc Feature(9)order(3951..3959,3993..3995,4002..4007,4014..4019)
Translation

Target ORF information:

RefSeq Version XM_011513428.2
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin G associated kinase (GAK), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011513428.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATGTG
GGGAGTGGCA GAGAGTATGC ATTAAAGAGG CTATTATCCA ATGAAGAGGA AAAGAACAGA
GCCATCATTC AAGAAGTTTG CTTCATGAAA AAGCTTTCCG GCCACCCGAA CATTGTCCAG
TTTTGTTCTG CAGCGTCTAT AGGAAAAGAG GAGTCAGACA CGGGGCAGGC TGAGTTCCTC
TTGCTCACAG AGCTCTGTAA AGGGCAGCTG GTGGAATTTT TGAAGAAAAT GGAATCTCGA
GGCCCCCTTT CGTGCGACAC GGTTCTGAAG ATCTTCTACC AGACGTGCCG CGCCGTGCAG
CACATGCACC GGCAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA GAACTTGTTG
CTTAGTAACC AAGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACGAC CATCTCGCAC
TACCCTGACT ACAGCTGGAG CGCCCAGAGG CGAGCCCTGG TGGAGGAAGA GATCACGAGG
AATACAACAC CAATGTATAG AACACCAGAA ATCATAGACT TGTATTCCAA CTTCCCGATC
GGCGAGAAGC AGGATATCTG GGCCCTGGGC TGCATCTTGT ACCTGCTGTG CTTCCGGCAG
CACCCTTTTG AGGATGGAGC GAAACTTCGA ATAGTCAATG GGAAGTACTC GATCCCCCCG
CACGACACGC AGTACACGGT CTTCCACAGC CTCATCCGCG CCATGCTGCA GGTGAACCCG
GAGGAGCGGC TGTCCATCGC CGAGGTGGTG CACCAGCTGC AGGAGATCGC GGCCGCCCGC
AACGTGAACC CCAAGTCTCC CATCACAGAG CTCCTGGAGC AGAATGGAGG CTACGGGAGC
GCCACACTGT CCCGAGGGCC ACCCCCTCCC GTGGGCCCCG CTGGCAGTGG CTACAGTGGA
GGCCTGGCGC TGGCGGAGTA CGACCAGCCG TATGGCGGCT TCCTGGACAT TCTGCGGGGT
GGGACAGAGC GGCTCTTCAC CAACCTCAAG GACACCTCCT CCAAGGTCAT CCAGTCCGTC
GCTAATTATG CAAAGGGTGA CCTGGACATA TCTTACATCA CATCCAGAAT TGCAGTGATG
TCATTCCCAG CAGAAGGTGT GGAGTCAGCG CTCAAAAACA ACATCGAAGA TGTGCGGTTG
TTCCTGGACT CCAAGCACCC AGGGCACTAT GCCGTCTACA ACCTGTCCCC GAGGACCTAC
CGGCCCTCCA GGTTCCACAA CCGGGTCTCC GAGTGTGGCT GGGCAGCACG GCGGGCCCCA
CACCTGCACA CCCTGTACAA CATCTGCAGG AACATGCACG CCTGGCTGCG GCAGGACCAC
AAGAACGTCT GCGTCGTGCA CTGCATGGAC GGGAGAGCCG CGTCTGCTGT GGCCGTCTGC
TCCTTCCTGT GCTTCTGCCG TCTCTTCAGC ACCGCGGAGG CCGCCGTGTA CATGTTCAGC
ATGAAGCGCT GCCCACCAGG CATCTGGCCA TCCCACAAAA GGTACATCGA GTACATGTGT
GACATGGTGG CGGAGGAGCC CATCACACCC CACAGCAAGC CCATCCTGGT GAGGGCCGTG
GTCATGACAC CCGTGCCGCT GTTCAGCAAG CAGAGGAGCG GCTGCAGGCC CTTCTGCGAG
GTCTACGTGG GGGACGAGCG TGTGGCCAGC ACCTCCCAGG AGTACGACAA GATGCGGGAC
TTTAAGATTG AAGATGGCAA AGCGGTGATT CCCCTGGGCG TCACGGTGCA AGGAGACGTG
CTCATCGTCA TCTATCACGC CCGGTCCACT CTGGGCGGCC GGCTGCAGGC CAAGATGGCA
TCCATGAAGA TGTTCCAGAT TCAGTTCCAC ACGGGGTTTG TGCCTCGGAA CGCCACCACT
GTGAAATTTG CCAAGTATGA CCTGGACGCG TGTGACATTC AAGAAAAATA CCCGGATTTA
TTTCAAGTGA ACCTGGAAGT GGAGGTGGAG CCCAGGGACA GGCCGAGCCG GGAAGCCCCA
CCATGGGAGA ACTCGAGCAT GAGGGGGCTG AACCCCAAAA TCCTGTTTTC CAGCCGGGAG
GAGCAGCAAG ACATTCTGTC TAAGTTTGAA GAGAAGGAGG CAGAGACTGG TGCAGAAAAT
GCCTCTTCCA AGGAGAGCGA GTCTGCCCTG ATGGAGGACA GAGACGAGAG TGAGGTGTCA
GATGAAGGGG GATCCCCGAT CTCCAGCGAG GGCCAGGAAC CCAGGGCCGA CCCAGAGCCC
CCCGGCCTGG CAGCAGGGCT GGTGCAGCAG GACTTGGTTT TTGAGGTGGA GACACCGGCT
GTGCTGCCAG AGCCTGTGCC ACAGGAAGAC GGGGTCGACC TCCTGGGCCT GCACTCCGAG
GTGGGCGCAG GGCCAGCTGT ACCCCCGCAG GCCTGCAAGG CCCCCTCCAG CAACACCGAC
CTGCTCAGCT GCCTCCTTGG GCCCCCTGAG GCCGCCTCCC AGGGGCCCCC GGAGGATCTG
CTCAGCGAGG ACCCGCTGCT CCTGGCAAGC CCGGCCCCTC CCCTGAGCGT GCAGAGCACC
CCAAGAGGAG GGCCCCCTGC CGCTGCTGAC CCCTTTGGCC CGCTTCTGCC GTCTTCAGGC
AACAACTCCC AGCCCTGCTC CAATCCTGAT CTCTTCGGCG AATTTCTCAA TTCGGACTCT
GTGACCGTCC CACCATCCTT CCCGTCTGCC CACAGTGCTC CGCCCCCATC CTGCAGCGCC
GACTTCCTGC ACCTGGGGGA TCTGCCAGGA GAGCCCAGCA AGATGACAGC CTCGTCCAGC
AACCCAGACC TGCTGGGAGG ATGGGCTGCC TGGACCGAGA CTGCAGCATC GGCAGTGGCC
CCCACGCCAG CCACAGAAGG CCCCCTCTTC TCTCCTGGAG GTCAGCCGGC CCCTTGTGGC
TCTCAGGCCA GCTGGACCAA GTCTCAGAAC CCGGACCCAT TTGCTGACCT TGGCGACCTC
AGCTCCGGCC TCCAAGACCC CCAAGCCCAG AGCACAGTGA GCCCAAGGGG ACAGCGTGTC
TGCACCTGTT CCAGGCGACT GCCAACTGGC AAGCTAAAAC CGGGAGTTGC TGACACTGGC
ACTGCTGCCA GCCCCCACCG GCATTGTGGC TCACCAGCTG GATTCCCTCC TGGGGGCTTC
ATTCCCAAAA CGGCCACCAC GCCCAAAGGC AGCAGCTCCT GGCAGACAAG TCGGCCGCCA
GCCCAGGGCG CCTCATGGCC CCCTCAGGCC AAGCCGCCCC CCAAAGCCTG CACACAGCCA
AGGCCTAACT ATGCCTCGAA CTTCAGTGTG ATCGGGGCGC GGGAGGAGCG GGGGGTCCGC
GCACCCAGCT TTGCTCAAAA GCCAAAAGTC TCTGAGAACG ACTTTGAAGA TCTGTTGTCC
AATCAAGGCT TCTCCTCCAG GTCTGACAAG AAAGGGCCAA AGACCATTGC AGAGATGAGG
AAGCAGGACC TGGCTAAAGA CACGGACCCA CTCAAGCTGA AGCTCCTGGA CTGGATTGAG
GGCAAGGAGC GGAACATCCG GGCCCTGCTG TCCACGCTGC ACACAGTGCT GTGGGACGGG
GAGAGCCGCT GGACGCCCGT GGGCATGGCC GACCTGGTGG CTCCGGAGCA AGTGAAGAAG
CACTATCGCC GCGCGGTGCT GGCTGTGCAC CCCGACAAGG CTGCGGGGCA GCCGTACGAG
CAGCACGCCA AGATGATCTT CATGGAGCTG AATGACGCCT GGTCGGAGTT TGAGAACCAG
GGCTCCCGGC CCCTCTTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57907
Clone ID Related Accession (Same CDS sequence) XM_011513429.2
Accession Version XM_011513429.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3882bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product cyclin-G-associated kinase isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011513429.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGGACTTTG GTGTCGCTGT GGTCAGTGCA CAGAACTGTG GACATGGTTA TGTACGTTCT 
CCTTTAACAA GACAACTGCA GAAAAAGCTT TCCGGCCACC CGAACATTGT CCAGTTTTGT
TCTGCAGCGT CTATAGGAAA AGAGGAGTCA GACACGGGGC AGGCTGAGTT CCTCTTGCTC
ACAGAGCTCT GTAAAGGGCA GCTGGTGGAA TTTTTGAAGA AAATGGAATC TCGAGGCCCC
CTTTCGTGCG ACACGGTTCT GAAGATCTTC TACCAGACGT GCCGCGCCGT GCAGCACATG
CACCGGCAGA AGCCGCCCAT CATCCACAGG GACCTCAAGG TTGAGAACTT GTTGCTTAGT
AACCAAGGGA CCATTAAGCT GTGTGACTTT GGCAGTGCCA CGACCATCTC GCACTACCCT
GACTACAGCT GGAGCGCCCA GAGGCGAGCC CTGGTGGAGG AAGAGATCAC GAGGAATACA
ACACCAATGT ATAGAACACC AGAAATCATA GACTTGTATT CCAACTTCCC GATCGGCGAG
AAGCAGGATA TCTGGGCCCT GGGCTGCATC TTGTACCTGC TGTGCTTCCG GCAGCACCCT
TTTGAGGATG GAGCGAAACT TCGAATAGTC AATGGGAAGT ACTCGATCCC CCCGCACGAC
ACGCAGTACA CGGTCTTCCA CAGCCTCATC CGCGCCATGC TGCAGGTGAA CCCGGAGGAG
CGGCTGTCCA TCGCCGAGGT GGTGCACCAG CTGCAGGAGA TCGCGGCCGC CCGCAACGTG
AACCCCAAGT CTCCCATCAC AGAGCTCCTG GAGCAGAATG GAGGCTACGG GAGCGCCACA
CTGTCCCGAG GGCCACCCCC TCCCGTGGGC CCCGCTGGCA GTGGCTACAG TGGAGGCCTG
GCGCTGGCGG AGTACGACCA GCCGTATGGC GGCTTCCTGG ACATTCTGCG GGGTGGGACA
GAGCGGCTCT TCACCAACCT CAAGGACACC TCCTCCAAGG TCATCCAGTC CGTCGCTAAT
TATGCAAAGG GTGACCTGGA CATATCTTAC ATCACATCCA GAATTGCAGT GATGTCATTC
CCAGCAGAAG GTGTGGAGTC AGCGCTCAAA AACAACATCG AAGATGTGCG GTTGTTCCTG
GACTCCAAGC ACCCAGGGCA CTATGCCGTC TACAACCTGT CCCCGAGGAC CTACCGGCCC
TCCAGGTTCC ACAACCGGGT CTCCGAGTGT GGCTGGGCAG CACGGCGGGC CCCACACCTG
CACACCCTGT ACAACATCTG CAGGAACATG CACGCCTGGC TGCGGCAGGA CCACAAGAAC
GTCTGCGTCG TGCACTGCAT GGACGGGAGA GCCGCGTCTG CTGTGGCCGT CTGCTCCTTC
CTGTGCTTCT GCCGTCTCTT CAGCACCGCG GAGGCCGCCG TGTACATGTT CAGCATGAAG
CGCTGCCCAC CAGGCATCTG GCCATCCCAC AAAAGGTACA TCGAGTACAT GTGTGACATG
GTGGCGGAGG AGCCCATCAC ACCCCACAGC AAGCCCATCC TGGTGAGGGC CGTGGTCATG
ACACCCGTGC CGCTGTTCAG CAAGCAGAGG AGCGGCTGCA GGCCCTTCTG CGAGGTCTAC
GTGGGGGACG AGCGTGTGGC CAGCACCTCC CAGGAGTACG ACAAGATGCG GGACTTTAAG
ATTGAAGATG GCAAAGCGGT GATTCCCCTG GGCGTCACGG TGCAAGGAGA CGTGCTCATC
GTCATCTATC ACGCCCGGTC CACTCTGGGC GGCCGGCTGC AGGCCAAGAT GGCATCCATG
AAGATGTTCC AGATTCAGTT CCACACGGGG TTTGTGCCTC GGAACGCCAC CACTGTGAAA
TTTGCCAAGT ATGACCTGGA CGCGTGTGAC ATTCAAGAAA AATACCCGGA TTTATTTCAA
GTGAACCTGG AAGTGGAGGT GGAGCCCAGG GACAGGCCGA GCCGGGAAGC CCCACCATGG
GAGAACTCGA GCATGAGGGG GCTGAACCCC AAAATCCTGT TTTCCAGCCG GGAGGAGCAG
CAAGACATTC TGTCTAAGTT TGGGAAGCCG GAGCTTCCCC GGCAGCCTGG CTCCACGGCT
CAGTATGATG CTGGGGCAGG GTCCCCGGAA GCCGAACCCA CAGACTCTGA CTCACCGCCA
AGCAGCAGCG CGGACGCCAG TCGCTTCCTG CACACGCTGG ACTGGCAGGA AGAGAAGGAG
GCAGAGACTG GTGCAGAAAA TGCCTCTTCC AAGGAGAGCG AGTCTGCCCT GATGGAGGAC
AGAGACGAGA GTGAGGTGTC AGATGAAGGG GGATCCCCGA TCTCCAGCGA GGGCCAGGAA
CCCAGGGCCG ACCCAGAGCC CCCCGGCCTG GCAGCAGGGC TGGTGCAGCA GGACTTGGTT
TTTGAGGTGG AGACACCGGC TGTGCTGCCA GAGCCTGTGC CACAGGAAGA CGGGGTCGAC
CTCCTGGGCC TGCACTCCGA GGTGGGCGCA GGGCCAGCTG TACCCCCGCA GGCCTGCAAG
GCCCCCTCCA GCAACACCGA CCTGCTCAGC TGCCTCCTTG GGCCCCCTGA GGCCGCCTCC
CAGGGGCCCC CGGAGGATCT GCTCAGCGAG GACCCGCTGC TCCTGGCAAG CCCGGCCCCT
CCCCTGAGCG TGCAGAGCAC CCCAAGAGGA GGGCCCCCTG CCGCTGCTGA CCCCTTTGGC
CCGCTTCTGC CGTCTTCAGG CAACAACTCC CAGCCCTGCT CCAATCCTGA TCTCTTCGGC
GAATTTCTCA ATTCGGACTC TGTGACCGTC CCACCATCCT TCCCGTCTGC CCACAGTGCT
CCGCCCCCAT CCTGCAGCGC CGACTTCCTG CACCTGGGGG ATCTGCCAGG AGAGCCCAGC
AAGATGACAG CCTCGTCCAG CAACCCAGAC CTGCTGGGAG GATGGGCTGC CTGGACCGAG
ACTGCAGCAT CGGCAGTGGC CCCCACGCCA GCCACAGAAG GCCCCCTCTT CTCTCCTGGA
GGTCAGCCGG CCCCTTGTGG CTCTCAGGCC AGCTGGACCA AGTCTCAGAA CCCGGACCCA
TTTGCTGACC TTGGCGACCT CAGCTCCGGC CTCCAAGACC CCCAAGCCCA GAGCACAGTG
AGCCCAAGGG GACAGCGTGT CTGCACCTGT TCCAGGCGAC TGCCAACTGG CAAGCTAAAA
CCGGGAGTTG CTGACACTGG CACTGCTGCC AGCCCCCACC GGCATTGTGG CTCACCAGCT
GGATTCCCTC CTGGGGGCTT CATTCCCAAA ACGGCCACCA CGCCCAAAGG CAGCAGCTCC
TGGCAGACAA GTCGGCCGCC AGCCCAGGGC GCCTCATGGC CCCCTCAGGC CAAGCCGCCC
CCCAAAGCCT GCACACAGCC AAGGCCTAAC TATGCCTCGA ACTTCAGTGT GATCGGGGCG
CGGGAGGAGC GGGGGGTCCG CGCACCCAGC TTTGCTCAAA AGCCAAAAGT CTCTGAGAAC
GACTTTGAAG ATCTGTTGTC CAATCAAGGC TTCTCCTCCA GGTCTGACAA GAAAGGGCCA
AAGACCATTG CAGAGATGAG GAAGCAGGAC CTGGCTAAAG ACACGGACCC ACTCAAGCTG
AAGCTCCTGG ACTGGATTGA GGGCAAGGAG CGGAACATCC GGGCCCTGCT GTCCACGCTG
CACACAGTGC TGTGGGACGG GGAGAGCCGC TGGACGCCCG TGGGCATGGC CGACCTGGTG
GCTCCGGAGC AAGTGAAGAA GCACTATCGC CGCGCGGTGC TGGCTGTGCA CCCCGACAAG
GCTGCGGGGC AGCCGTACGA GCAGCACGCC AAGATGATCT TCATGGAGCT GAATGACGCC
TGGTCGGAGT TTGAGAACCA GGGCTCCCGG CCCCTCTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011511731.1
CDS652..4533
Misc Feature(1)721..1392
Misc Feature(2)724..1425
Misc Feature(3)2173..2589
Misc Feature(4)<4375..4509
Misc Feature(5)order(4420..4428,4462..4464,4471..4476,4483..4488)
Translation

Target ORF information:

RefSeq Version XM_011513429.2
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin G associated kinase (GAK), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011513429.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGGACTTTG GTGTCGCTGT GGTCAGTGCA CAGAACTGTG GACATGGTTA TGTACGTTCT 
CCTTTAACAA GACAACTGCA GAAAAAGCTT TCCGGCCACC CGAACATTGT CCAGTTTTGT
TCTGCAGCGT CTATAGGAAA AGAGGAGTCA GACACGGGGC AGGCTGAGTT CCTCTTGCTC
ACAGAGCTCT GTAAAGGGCA GCTGGTGGAA TTTTTGAAGA AAATGGAATC TCGAGGCCCC
CTTTCGTGCG ACACGGTTCT GAAGATCTTC TACCAGACGT GCCGCGCCGT GCAGCACATG
CACCGGCAGA AGCCGCCCAT CATCCACAGG GACCTCAAGG TTGAGAACTT GTTGCTTAGT
AACCAAGGGA CCATTAAGCT GTGTGACTTT GGCAGTGCCA CGACCATCTC GCACTACCCT
GACTACAGCT GGAGCGCCCA GAGGCGAGCC CTGGTGGAGG AAGAGATCAC GAGGAATACA
ACACCAATGT ATAGAACACC AGAAATCATA GACTTGTATT CCAACTTCCC GATCGGCGAG
AAGCAGGATA TCTGGGCCCT GGGCTGCATC TTGTACCTGC TGTGCTTCCG GCAGCACCCT
TTTGAGGATG GAGCGAAACT TCGAATAGTC AATGGGAAGT ACTCGATCCC CCCGCACGAC
ACGCAGTACA CGGTCTTCCA CAGCCTCATC CGCGCCATGC TGCAGGTGAA CCCGGAGGAG
CGGCTGTCCA TCGCCGAGGT GGTGCACCAG CTGCAGGAGA TCGCGGCCGC CCGCAACGTG
AACCCCAAGT CTCCCATCAC AGAGCTCCTG GAGCAGAATG GAGGCTACGG GAGCGCCACA
CTGTCCCGAG GGCCACCCCC TCCCGTGGGC CCCGCTGGCA GTGGCTACAG TGGAGGCCTG
GCGCTGGCGG AGTACGACCA GCCGTATGGC GGCTTCCTGG ACATTCTGCG GGGTGGGACA
GAGCGGCTCT TCACCAACCT CAAGGACACC TCCTCCAAGG TCATCCAGTC CGTCGCTAAT
TATGCAAAGG GTGACCTGGA CATATCTTAC ATCACATCCA GAATTGCAGT GATGTCATTC
CCAGCAGAAG GTGTGGAGTC AGCGCTCAAA AACAACATCG AAGATGTGCG GTTGTTCCTG
GACTCCAAGC ACCCAGGGCA CTATGCCGTC TACAACCTGT CCCCGAGGAC CTACCGGCCC
TCCAGGTTCC ACAACCGGGT CTCCGAGTGT GGCTGGGCAG CACGGCGGGC CCCACACCTG
CACACCCTGT ACAACATCTG CAGGAACATG CACGCCTGGC TGCGGCAGGA CCACAAGAAC
GTCTGCGTCG TGCACTGCAT GGACGGGAGA GCCGCGTCTG CTGTGGCCGT CTGCTCCTTC
CTGTGCTTCT GCCGTCTCTT CAGCACCGCG GAGGCCGCCG TGTACATGTT CAGCATGAAG
CGCTGCCCAC CAGGCATCTG GCCATCCCAC AAAAGGTACA TCGAGTACAT GTGTGACATG
GTGGCGGAGG AGCCCATCAC ACCCCACAGC AAGCCCATCC TGGTGAGGGC CGTGGTCATG
ACACCCGTGC CGCTGTTCAG CAAGCAGAGG AGCGGCTGCA GGCCCTTCTG CGAGGTCTAC
GTGGGGGACG AGCGTGTGGC CAGCACCTCC CAGGAGTACG ACAAGATGCG GGACTTTAAG
ATTGAAGATG GCAAAGCGGT GATTCCCCTG GGCGTCACGG TGCAAGGAGA CGTGCTCATC
GTCATCTATC ACGCCCGGTC CACTCTGGGC GGCCGGCTGC AGGCCAAGAT GGCATCCATG
AAGATGTTCC AGATTCAGTT CCACACGGGG TTTGTGCCTC GGAACGCCAC CACTGTGAAA
TTTGCCAAGT ATGACCTGGA CGCGTGTGAC ATTCAAGAAA AATACCCGGA TTTATTTCAA
GTGAACCTGG AAGTGGAGGT GGAGCCCAGG GACAGGCCGA GCCGGGAAGC CCCACCATGG
GAGAACTCGA GCATGAGGGG GCTGAACCCC AAAATCCTGT TTTCCAGCCG GGAGGAGCAG
CAAGACATTC TGTCTAAGTT TGGGAAGCCG GAGCTTCCCC GGCAGCCTGG CTCCACGGCT
CAGTATGATG CTGGGGCAGG GTCCCCGGAA GCCGAACCCA CAGACTCTGA CTCACCGCCA
AGCAGCAGCG CGGACGCCAG TCGCTTCCTG CACACGCTGG ACTGGCAGGA AGAGAAGGAG
GCAGAGACTG GTGCAGAAAA TGCCTCTTCC AAGGAGAGCG AGTCTGCCCT GATGGAGGAC
AGAGACGAGA GTGAGGTGTC AGATGAAGGG GGATCCCCGA TCTCCAGCGA GGGCCAGGAA
CCCAGGGCCG ACCCAGAGCC CCCCGGCCTG GCAGCAGGGC TGGTGCAGCA GGACTTGGTT
TTTGAGGTGG AGACACCGGC TGTGCTGCCA GAGCCTGTGC CACAGGAAGA CGGGGTCGAC
CTCCTGGGCC TGCACTCCGA GGTGGGCGCA GGGCCAGCTG TACCCCCGCA GGCCTGCAAG
GCCCCCTCCA GCAACACCGA CCTGCTCAGC TGCCTCCTTG GGCCCCCTGA GGCCGCCTCC
CAGGGGCCCC CGGAGGATCT GCTCAGCGAG GACCCGCTGC TCCTGGCAAG CCCGGCCCCT
CCCCTGAGCG TGCAGAGCAC CCCAAGAGGA GGGCCCCCTG CCGCTGCTGA CCCCTTTGGC
CCGCTTCTGC CGTCTTCAGG CAACAACTCC CAGCCCTGCT CCAATCCTGA TCTCTTCGGC
GAATTTCTCA ATTCGGACTC TGTGACCGTC CCACCATCCT TCCCGTCTGC CCACAGTGCT
CCGCCCCCAT CCTGCAGCGC CGACTTCCTG CACCTGGGGG ATCTGCCAGG AGAGCCCAGC
AAGATGACAG CCTCGTCCAG CAACCCAGAC CTGCTGGGAG GATGGGCTGC CTGGACCGAG
ACTGCAGCAT CGGCAGTGGC CCCCACGCCA GCCACAGAAG GCCCCCTCTT CTCTCCTGGA
GGTCAGCCGG CCCCTTGTGG CTCTCAGGCC AGCTGGACCA AGTCTCAGAA CCCGGACCCA
TTTGCTGACC TTGGCGACCT CAGCTCCGGC CTCCAAGACC CCCAAGCCCA GAGCACAGTG
AGCCCAAGGG GACAGCGTGT CTGCACCTGT TCCAGGCGAC TGCCAACTGG CAAGCTAAAA
CCGGGAGTTG CTGACACTGG CACTGCTGCC AGCCCCCACC GGCATTGTGG CTCACCAGCT
GGATTCCCTC CTGGGGGCTT CATTCCCAAA ACGGCCACCA CGCCCAAAGG CAGCAGCTCC
TGGCAGACAA GTCGGCCGCC AGCCCAGGGC GCCTCATGGC CCCCTCAGGC CAAGCCGCCC
CCCAAAGCCT GCACACAGCC AAGGCCTAAC TATGCCTCGA ACTTCAGTGT GATCGGGGCG
CGGGAGGAGC GGGGGGTCCG CGCACCCAGC TTTGCTCAAA AGCCAAAAGT CTCTGAGAAC
GACTTTGAAG ATCTGTTGTC CAATCAAGGC TTCTCCTCCA GGTCTGACAA GAAAGGGCCA
AAGACCATTG CAGAGATGAG GAAGCAGGAC CTGGCTAAAG ACACGGACCC ACTCAAGCTG
AAGCTCCTGG ACTGGATTGA GGGCAAGGAG CGGAACATCC GGGCCCTGCT GTCCACGCTG
CACACAGTGC TGTGGGACGG GGAGAGCCGC TGGACGCCCG TGGGCATGGC CGACCTGGTG
GCTCCGGAGC AAGTGAAGAA GCACTATCGC CGCGCGGTGC TGGCTGTGCA CCCCGACAAG
GCTGCGGGGC AGCCGTACGA GCAGCACGCC AAGATGATCT TCATGGAGCT GAATGACGCC
TGGTCGGAGT TTGAGAACCA GGGCTCCCGG CCCCTCTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57908
Clone ID Related Accession (Same CDS sequence) XM_011513430.1
Accession Version XM_011513430.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3831bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product cyclin-G-associated kinase isoform X8
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGGCAG CTGGTGGAAT TTTTGAAGAA AATGGAATCT
CGAGGCCCCC TTTCGTGCGA CACGGTTCTG AAGATCTTCT ACCAGACGTG CCGCGCCGTG
CAGCACATGC ACCGGCAGAA GCCGCCCATC ATCCACAGGG ACCTCAAGGT TGAGAACTTG
TTGCTTAGTA ACCAAGGGAC CATTAAGCTG TGTGACTTTG GCAGTGCCAC GACCATCTCG
CACTACCCTG ACTACAGCTG GAGCGCCCAG AGGCGAGCCC TGGTGGAGGA AGAGATCACG
AGGAATACAA CACCAATGTA TAGAACACCA GAAATCATAG ACTTGTATTC CAACTTCCCG
ATCGGCGAGA AGCAGGATAT CTGGGCCCTG GGCTGCATCT TGTACCTGCT GTGCTTCCGG
CAGCACCCTT TTGAGGATGG AGCGAAACTT CGAATAGTCA ATGGGAAGTA CTCGATCCCC
CCGCACGACA CGCAGTACAC GGTCTTCCAC AGCCTCATCC GCGCCATGCT GCAGGTGAAC
CCGGAGGAGC GGCTGTCCAT CGCCGAGGTG GTGCACCAGC TGCAGGAGAT CGCGGCCGCC
CGCAACGTGA ACCCCAAGTC TCCCATCACA GAGCTCCTGG AGCAGAATGG AGGCTACGGG
AGCGCCACAC TGTCCCGAGG GCCACCCCCT CCCGTGGGCC CCGCTGGCAG TGGCTACAGT
GGAGGCCTGG CGCTGGCGGA GTACGACCAG CCGTATGGCG GCTTCCTGGA CATTCTGCGG
GGTGGGACAG AGCGGCTCTT CACCAACCTC AAGGACACCT CCTCCAAGGT CATCCAGTCC
GTCGCTAATT ATGCAAAGGG TGACCTGGAC ATATCTTACA TCACATCCAG AATTGCAGTG
ATGTCATTCC CAGCAGAAGG TGTGGAGTCA GCGCTCAAAA ACAACATCGA AGATGTGCGG
TTGTTCCTGG ACTCCAAGCA CCCAGGGCAC TATGCCGTCT ACAACCTGTC CCCGAGGACC
TACCGGCCCT CCAGGTTCCA CAACCGGGTC TCCGAGTGTG GCTGGGCAGC ACGGCGGGCC
CCACACCTGC ACACCCTGTA CAACATCTGC AGGAACATGC ACGCCTGGCT GCGGCAGGAC
CACAAGAACG TCTGCGTCGT GCACTGCATG GACGGGAGAG CCGCGTCTGC TGTGGCCGTC
TGCTCCTTCC TGTGCTTCTG CCGTCTCTTC AGCACCGCGG AGGCCGCCGT GTACATGTTC
AGCATGAAGC GCTGCCCACC AGGCATCTGG CCATCCCACA AAAGGTACAT CGAGTACATG
TGTGACATGG TGGCGGAGGA GCCCATCACA CCCCACAGCA AGCCCATCCT GGTGAGGGCC
GTGGTCATGA CACCCGTGCC GCTGTTCAGC AAGCAGAGGA GCGGCTGCAG GCCCTTCTGC
GAGGTCTACG TGGGGGACGA GCGTGTGGCC AGCACCTCCC AGGAGTACGA CAAGATGCGG
GACTTTAAGA TTGAAGATGG CAAAGCGGTG ATTCCCCTGG GCGTCACGGT GCAAGGAGAC
GTGCTCATCG TCATCTATCA CGCCCGGTCC ACTCTGGGCG GCCGGCTGCA GGCCAAGATG
GCATCCATGA AGATGTTCCA GATTCAGTTC CACACGGGGT TTGTGCCTCG GAACGCCACC
ACTGTGAAAT TTGCCAAGTA TGACCTGGAC GCGTGTGACA TTCAAGAAAA ATACCCGGAT
TTATTTCAAG TGAACCTGGA AGTGGAGGTG GAGCCCAGGG ACAGGCCGAG CCGGGAAGCC
CCACCATGGG AGAACTCGAG CATGAGGGGG CTGAACCCCA AAATCCTGTT TTCCAGCCGG
GAGGAGCAGC AAGACATTCT GTCTAAGTTT GGGAAGCCGG AGCTTCCCCG GCAGCCTGGC
TCCACGGCTC AGTATGATGC TGGGGCAGGG TCCCCGGAAG CCGAACCCAC AGACTCTGAC
TCACCGCCAA GCAGCAGCGC GGACGCCAGT CGCTTCCTGC ACACGCTGGA CTGGCAGGAA
GAGAAGGAGG CAGAGACTGG TGCAGAAAAT GCCTCTTCCA AGGAGAGCGA GTCTGCCCTG
ATGGAGGACA GAGACGAGAG TGAGGTGTCA GATGAAGGGG GATCCCCGAT CTCCAGCGAG
GGCCAGGAAC CCAGGGCCGA CCCAGAGCCC CCCGGCCTGG CAGCAGGGCT GGTGCAGCAG
GACTTGGTTT TTGAGGTGGA GACACCGGCT GTGCTGCCAG AGCCTGTGCC ACAGGAAGAC
GGGGTCGACC TCCTGGGCCT GCACTCCGAG GTGGGCGCAG GGCCAGCTGT ACCCCCGCAG
GCCTGCAAGG CCCCCTCCAG CAACACCGAC CTGCTCAGCT GCCTCCTTGG GCCCCCTGAG
GCCGCCTCCC AGGGGCCCCC GGAGGATCTG CTCAGCGAGG ACCCGCTGCT CCTGGCAAGC
CCGGCCCCTC CCCTGAGCGT GCAGAGCACC CCAAGAGGAG GGCCCCCTGC CGCTGCTGAC
CCCTTTGGCC CGCTTCTGCC GTCTTCAGGC AACAACTCCC AGCCCTGCTC CAATCCTGAT
CTCTTCGGCG AATTTCTCAA TTCGGACTCT GTGACCGTCC CACCATCCTT CCCGTCTGCC
CACAGTGCTC CGCCCCCATC CTGCAGCGCC GACTTCCTGC ACCTGGGGGA TCTGCCAGGA
GAGCCCAGCA AGATGACAGC CTCGTCCAGC AACCCAGACC TGCTGGGAGG ATGGGCTGCC
TGGACCGAGA CTGCAGCATC GGCAGTGGCC CCCACGCCAG CCACAGAAGG CCCCCTCTTC
TCTCCTGGAG GTCAGCCGGC CCCTTGTGGC TCTCAGGCCA GCTGGACCAA GTCTCAGAAC
CCGGACCCAT TTGCTGACCT TGGCGACCTC AGCTCCGGCC TCCAAGACCC CCAAGCCCAG
AGCACAGTGA GCCCAAGGGG ACAGCGTGTC TGCACCTGTT CCAGGCGACT GCCAACTGGC
AAGCTAAAAC CGGGAGTTGC TGACACTGGC ACTGCTGCCA GCCCCCACCG GCATTGTGGC
TCACCAGCTG GATTCCCTCC TGGGGGCTTC ATTCCCAAAA CGGCCACCAC GCCCAAAGGC
AGCAGCTCCT GGCAGACAAG TCGGCCGCCA GCCCAGGGCG CCTCATGGCC CCCTCAGGCC
AAGCCGCCCC CCAAAGCCTG CACACAGCCA AGGCCTAACT ATGCCTCGAA CTTCAGTGTG
ATCGGGGCGC GGGAGGAGCG GGGGGTCCGC GCACCCAGCT TTGCTCAAAA GCCAAAAGTC
TCTGAGAACG ACTTTGAAGA TCTGTTGTCC AATCAAGGCT TCTCCTCCAG GTCTGACAAG
AAAGGGCCAA AGACCATTGC AGAGATGAGG AAGCAGGACC TGGCTAAAGA CACGGACCCA
CTCAAGCTGA AGCTCCTGGA CTGGATTGAG GGCAAGGAGC GGAACATCCG GGCCCTGCTG
TCCACGCTGC ACACAGTGCT GTGGGACGGG GAGAGCCGCT GGACGCCCGT GGGCATGGCC
GACCTGGTGG CTCCGGAGCA AGTGAAGAAG CACTATCGCC GCGCGGTGCT GGCTGTGCAC
CCCGACAAGG CTGCGGGGCA GCCGTACGAG CAGCACGCCA AGATGATCTT CATGGAGCTG
AATGACGCCT GGTCGGAGTT TGAGAACCAG GGCTCCCGGC CCCTCTTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011511732.1
CDS128..3958
Misc Feature(1)<263..850
Misc Feature(2)<284..817
Misc Feature(3)1598..2014
Misc Feature(4)<3800..3934
Misc Feature(5)order(3845..3853,3887..3889,3896..3901,3908..3913)
Translation

Target ORF information:

RefSeq Version XM_011513430.1
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin G associated kinase (GAK), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011513430.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGGCAG CTGGTGGAAT TTTTGAAGAA AATGGAATCT
CGAGGCCCCC TTTCGTGCGA CACGGTTCTG AAGATCTTCT ACCAGACGTG CCGCGCCGTG
CAGCACATGC ACCGGCAGAA GCCGCCCATC ATCCACAGGG ACCTCAAGGT TGAGAACTTG
TTGCTTAGTA ACCAAGGGAC CATTAAGCTG TGTGACTTTG GCAGTGCCAC GACCATCTCG
CACTACCCTG ACTACAGCTG GAGCGCCCAG AGGCGAGCCC TGGTGGAGGA AGAGATCACG
AGGAATACAA CACCAATGTA TAGAACACCA GAAATCATAG ACTTGTATTC CAACTTCCCG
ATCGGCGAGA AGCAGGATAT CTGGGCCCTG GGCTGCATCT TGTACCTGCT GTGCTTCCGG
CAGCACCCTT TTGAGGATGG AGCGAAACTT CGAATAGTCA ATGGGAAGTA CTCGATCCCC
CCGCACGACA CGCAGTACAC GGTCTTCCAC AGCCTCATCC GCGCCATGCT GCAGGTGAAC
CCGGAGGAGC GGCTGTCCAT CGCCGAGGTG GTGCACCAGC TGCAGGAGAT CGCGGCCGCC
CGCAACGTGA ACCCCAAGTC TCCCATCACA GAGCTCCTGG AGCAGAATGG AGGCTACGGG
AGCGCCACAC TGTCCCGAGG GCCACCCCCT CCCGTGGGCC CCGCTGGCAG TGGCTACAGT
GGAGGCCTGG CGCTGGCGGA GTACGACCAG CCGTATGGCG GCTTCCTGGA CATTCTGCGG
GGTGGGACAG AGCGGCTCTT CACCAACCTC AAGGACACCT CCTCCAAGGT CATCCAGTCC
GTCGCTAATT ATGCAAAGGG TGACCTGGAC ATATCTTACA TCACATCCAG AATTGCAGTG
ATGTCATTCC CAGCAGAAGG TGTGGAGTCA GCGCTCAAAA ACAACATCGA AGATGTGCGG
TTGTTCCTGG ACTCCAAGCA CCCAGGGCAC TATGCCGTCT ACAACCTGTC CCCGAGGACC
TACCGGCCCT CCAGGTTCCA CAACCGGGTC TCCGAGTGTG GCTGGGCAGC ACGGCGGGCC
CCACACCTGC ACACCCTGTA CAACATCTGC AGGAACATGC ACGCCTGGCT GCGGCAGGAC
CACAAGAACG TCTGCGTCGT GCACTGCATG GACGGGAGAG CCGCGTCTGC TGTGGCCGTC
TGCTCCTTCC TGTGCTTCTG CCGTCTCTTC AGCACCGCGG AGGCCGCCGT GTACATGTTC
AGCATGAAGC GCTGCCCACC AGGCATCTGG CCATCCCACA AAAGGTACAT CGAGTACATG
TGTGACATGG TGGCGGAGGA GCCCATCACA CCCCACAGCA AGCCCATCCT GGTGAGGGCC
GTGGTCATGA CACCCGTGCC GCTGTTCAGC AAGCAGAGGA GCGGCTGCAG GCCCTTCTGC
GAGGTCTACG TGGGGGACGA GCGTGTGGCC AGCACCTCCC AGGAGTACGA CAAGATGCGG
GACTTTAAGA TTGAAGATGG CAAAGCGGTG ATTCCCCTGG GCGTCACGGT GCAAGGAGAC
GTGCTCATCG TCATCTATCA CGCCCGGTCC ACTCTGGGCG GCCGGCTGCA GGCCAAGATG
GCATCCATGA AGATGTTCCA GATTCAGTTC CACACGGGGT TTGTGCCTCG GAACGCCACC
ACTGTGAAAT TTGCCAAGTA TGACCTGGAC GCGTGTGACA TTCAAGAAAA ATACCCGGAT
TTATTTCAAG TGAACCTGGA AGTGGAGGTG GAGCCCAGGG ACAGGCCGAG CCGGGAAGCC
CCACCATGGG AGAACTCGAG CATGAGGGGG CTGAACCCCA AAATCCTGTT TTCCAGCCGG
GAGGAGCAGC AAGACATTCT GTCTAAGTTT GGGAAGCCGG AGCTTCCCCG GCAGCCTGGC
TCCACGGCTC AGTATGATGC TGGGGCAGGG TCCCCGGAAG CCGAACCCAC AGACTCTGAC
TCACCGCCAA GCAGCAGCGC GGACGCCAGT CGCTTCCTGC ACACGCTGGA CTGGCAGGAA
GAGAAGGAGG CAGAGACTGG TGCAGAAAAT GCCTCTTCCA AGGAGAGCGA GTCTGCCCTG
ATGGAGGACA GAGACGAGAG TGAGGTGTCA GATGAAGGGG GATCCCCGAT CTCCAGCGAG
GGCCAGGAAC CCAGGGCCGA CCCAGAGCCC CCCGGCCTGG CAGCAGGGCT GGTGCAGCAG
GACTTGGTTT TTGAGGTGGA GACACCGGCT GTGCTGCCAG AGCCTGTGCC ACAGGAAGAC
GGGGTCGACC TCCTGGGCCT GCACTCCGAG GTGGGCGCAG GGCCAGCTGT ACCCCCGCAG
GCCTGCAAGG CCCCCTCCAG CAACACCGAC CTGCTCAGCT GCCTCCTTGG GCCCCCTGAG
GCCGCCTCCC AGGGGCCCCC GGAGGATCTG CTCAGCGAGG ACCCGCTGCT CCTGGCAAGC
CCGGCCCCTC CCCTGAGCGT GCAGAGCACC CCAAGAGGAG GGCCCCCTGC CGCTGCTGAC
CCCTTTGGCC CGCTTCTGCC GTCTTCAGGC AACAACTCCC AGCCCTGCTC CAATCCTGAT
CTCTTCGGCG AATTTCTCAA TTCGGACTCT GTGACCGTCC CACCATCCTT CCCGTCTGCC
CACAGTGCTC CGCCCCCATC CTGCAGCGCC GACTTCCTGC ACCTGGGGGA TCTGCCAGGA
GAGCCCAGCA AGATGACAGC CTCGTCCAGC AACCCAGACC TGCTGGGAGG ATGGGCTGCC
TGGACCGAGA CTGCAGCATC GGCAGTGGCC CCCACGCCAG CCACAGAAGG CCCCCTCTTC
TCTCCTGGAG GTCAGCCGGC CCCTTGTGGC TCTCAGGCCA GCTGGACCAA GTCTCAGAAC
CCGGACCCAT TTGCTGACCT TGGCGACCTC AGCTCCGGCC TCCAAGACCC CCAAGCCCAG
AGCACAGTGA GCCCAAGGGG ACAGCGTGTC TGCACCTGTT CCAGGCGACT GCCAACTGGC
AAGCTAAAAC CGGGAGTTGC TGACACTGGC ACTGCTGCCA GCCCCCACCG GCATTGTGGC
TCACCAGCTG GATTCCCTCC TGGGGGCTTC ATTCCCAAAA CGGCCACCAC GCCCAAAGGC
AGCAGCTCCT GGCAGACAAG TCGGCCGCCA GCCCAGGGCG CCTCATGGCC CCCTCAGGCC
AAGCCGCCCC CCAAAGCCTG CACACAGCCA AGGCCTAACT ATGCCTCGAA CTTCAGTGTG
ATCGGGGCGC GGGAGGAGCG GGGGGTCCGC GCACCCAGCT TTGCTCAAAA GCCAAAAGTC
TCTGAGAACG ACTTTGAAGA TCTGTTGTCC AATCAAGGCT TCTCCTCCAG GTCTGACAAG
AAAGGGCCAA AGACCATTGC AGAGATGAGG AAGCAGGACC TGGCTAAAGA CACGGACCCA
CTCAAGCTGA AGCTCCTGGA CTGGATTGAG GGCAAGGAGC GGAACATCCG GGCCCTGCTG
TCCACGCTGC ACACAGTGCT GTGGGACGGG GAGAGCCGCT GGACGCCCGT GGGCATGGCC
GACCTGGTGG CTCCGGAGCA AGTGAAGAAG CACTATCGCC GCGCGGTGCT GGCTGTGCAC
CCCGACAAGG CTGCGGGGCA GCCGTACGAG CAGCACGCCA AGATGATCTT CATGGAGCTG
AATGACGCCT GGTCGGAGTT TGAGAACCAG GGCTCCCGGC CCCTCTTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57909
Clone ID Related Accession (Same CDS sequence) XM_011513431.2
Accession Version XM_011513431.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3819bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product cyclin-G-associated kinase isoform X9
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011513431.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATGTG
GGGAGTGGCA GAGAGTATGC ATTAAAGAGG CTATTATCCA ATGAAGAGGA AAAGAACAGA
GCCATCATTC AAGAAGTTTG CTTCATGAAA AAGCTTTCCG GCCACCCGAA CATTGTCCAG
TTTTGTTCTG CAGCGTCTAT AGGAAAAGAG GAGTCAGACA CGGGGCAGGC TGAGTTCCTC
TTGCTCACAG AGCTCTGTAA AGGGCAGCTG GTGGAATTTT TGAAGAAAAT GGAATCTCGA
GGCCCCCTTT CGTGCGACAC GGTTCTGAAG ATCTTCTACC AGACGTGCCG CGCCGTGCAG
CACATGCACC GGCAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA GAACTTGTTG
CTTAGTAACC AAGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACGAC CATCTCGCAC
TACCCTGACT ACAGCTGGAG CGCCCAGAGG CGAGCCCTGG TGGAGGAAGA GATCACGAGG
AATACAACAC CAATGTATAG AACACCAGAA ATCATAGACT TGTATTCCAA CTTCCCGATC
GGCGAGAAGC AGGATATCTG GGCCCTGGGC TGCATCTTGT ACCTGCTGTG CTTCCGGCAG
CACCCTTTTG AGGATGGAGC GAAACTTCGA ATAGTCAATG GGAAGTACTC GATCCCCCCG
CACGACACGC AGTACACGGT CTTCCACAGC CTCATCCGCG CCATGCTGCA GGTGAACCCG
GAGGAGCGGC TGTCCATCGC CGAGGTGGTG CACCAGCTGC AGGAGATCGC GGCCGCCCGC
AACGTGAACC CCAAGTCTCC CATCACAGAG CTCCTGGAGC AGAATGGAGG CTACGGGAGC
GCCACACTGT CCCGAGGGCC ACCCCCTCCC GTGGGCCCCG CTGGCAGTGG CTACAGTGGA
GGCCTGGCGC TGGCGGAGTA CGACCAGCCG TATGGCGGCT TCCTGGACAT TCTGCGGGGT
GGGACAGAGC GGCTCTTCAC CAACCTCAAG GACACCTCCT CCAAGGTCAT CCAGTCCGTC
GCTAATTATG CAAAGGGTGA CCTGGACATA TCTTACATCA CATCCAGAAT TGCAGTGATG
TCATTCCCAG CAGAAGGTGT GGAGTCAGCG CTCAAAAACA ACATCGAAGA TGTGCGGTTG
TTCCTGGACT CCAAGCACCC AGGGCACTAT GCCGTCTACA ACCTGTCCCC GAGGACCTAC
CGGCCCTCCA GGTTCCACAA CCGGGTCTCC GAGTGTGGCT GGGCAGCACG GCGGGCCCCA
CACCTGCACA CCCTGTACAA CATCTGCAGG AACATGCACG CCTGGCTGCG GCAGGACCAC
AAGAACGTCT GCGTCGTGCA CTGCATGGAC GGGAGAGCCG CGTCTGCTGT GGCCGTCTGC
TCCTTCCTGT GCTTCTGCCG TCTCTTCAGC ACCGCGGAGG CCGCCGTGTA CATGTTCAGC
ATGAAGCGCT GCCCACCAGG CATCTGGCCA TCCCACAAAA GGTACATCGA GTACATGTGT
GACATGGTGG CGGAGGAGCC CATCACACCC CACAGCAAGC CCATCCTGGT GAGGGCCGTG
GTCATGACAC CCGTGCCGCT GTTCAGCAAG CAGAGGAGCG GCTGCAGGCC CTTCTGCGAG
GTCTACGTGG GGGACGAGCG TGTGGCCAGC ACCTCCCAGG AGTACGACAA GATGCGGGAC
TTTAAGATTG AAGATGGCAA AGCGGTGATT CCCCTGGGCG TCACGGTGCA AGGAGACGTG
CTCATCGTCA TCTATCACGC CCGGTCCACT CTGGGCGGCC GGCTGCAGGC CAAGATGGCA
TCCATGAAGA TGTTCCAGAT TCAGTTCCAC ACGGGGTTTG TGCCTCGGAA CGCCACCACT
GTGAAATTTG CCAAGTATGA CCTGGACGCG TGTGACATTC AAGAAAAATA CCCGGATTTA
TTTCAAGTGA ACCTGGAAGT GGAGGTGGAG CCCAGGGACA GGCCGAGCCG GGAAGCCCCA
CCATGGGAGA ACTCGAGCAT GAGGGGGCTG AACCCCAAAA TCCTGTTTTC CAGCCGGGAG
GAGCAGCAAG ACATTCTGTC TAAGTTTGGG AAGCCGGAGC TTCCCCGGCA GCCTGGCTCC
ACGGCTCAGT ATGATGCTGG GGCAGGGTCC CCGGAAGCCG AACCCACAGA CTCTGACTCA
CCGCCAAGCA GCAGCGCGGA CGCCAGTCGC TTCCTGCACA CGCTGGACTG GCAGGAAGAG
AAGGAGGCAG AGACTGGTGC AGAAAATGCC TCTTCCAAGG AGAGCGAGTC TGCCCTGATG
GAGGACAGAG ACGAGAGTGA GGTGTCAGAT GAAGGGGGAT CCCCGATCTC CAGCGAGGGC
CAGGAACCCA GGGCCGACCC AGAGCCCCCC GGCCTGGCAG CAGGGCTGGT GCAGCAGGAC
TTGGTTTTTG AGGTGGAGAC ACCGGCTGTG CTGCCAGAGC CTGTGCCACA GGAAGACGGG
GTCGACCTCC TGGGCCTGCA CTCCGAGGTG GGCGCAGGGC CAGCTGTACC CCCGCAGGCC
TGCAAGGCCC CCTCCAGCAA CACCGACCTG CTCAGCTGCC TCCTTGGGCC CCCTGAGGCC
GCCTCCCAGG GGCCCCCGGA GGATCTGCTC AGCGAGGACC CGCTGCTCCT GGCAAGCCCG
GCCCCTCCCC TGAGCGTGCA GAGCACCCCA AGAGGAGGGC CCCCTGCCGC TGCTGACCCC
TTTGGCCCGC TTCTGCCGTC TTCAGGCAAC AACTCCCAGC CCTGCTCCAA TCCTGATCTC
TTCGGCGAAT TTCTCAATTC GGACTCTGTG ACCGTCCCAC CATCCTTCCC GTCTGCCCAC
AGTGCTCCGC CCCCATCCTG CAGCGCCGAC TTCCTGCACC TGGGGGATCT GCCAGGAGAG
CCCAGCAAGA TGACAGCCTC GTCCAGCAAC CCAGACCTGC TGGGAGGATG GGCTGCCTGG
ACCGAGACTG CAGCATCGGC AGTGGCCCCC ACGCCAGCCA CAGAAGGCTC ACCAGCTGGA
TTCCCTCCTG GGGGCTTCAT TCCCAAAACG GCCACCACGC CCAAAGGCAG CAGCTCCTGG
CAGACAAGTC GGCCGCCAGC CCAGGGCGCC TCATGGCCCC CTCAGGCCAA GCCGCCCCCC
AAAGCCTGCA CACAGCCAAG GCCTAACTAT GCCTCGAACT TCAGTGTGAT CGGGGCGCGG
GAGGAGCGGG GGGTCCGCGC ACCCAGCTTT GCTCAAAAGC CAAAAGTCTC TGAGAACGAC
TTTGAAGATC TGTTGTCCAA TCAAGGCTTC TCCTCCAGGT CTGACAAGAA AGGGCCAAAG
ACCATTGCAG AGATGAGGAA GCAGGACCTG GCTAAAGACA CGGACCCACT CAAGCTGAAG
CTCCTGGACT GGATTGAGGG CAAGGAGCGG AACATCCGGG CCCTGCTGTC CACGCTGCAC
ACAGTGCTGT GGGACGGGGA GAGCCGCTGG ACGCCCGTGG GCATGGCCGA CCTGGTGGCT
CCGGAGCAAG TGAAGAAGCA CTATCGCCGC GCGGTGCTGG CTGTGCACCC CGACAAGGCT
GCGGGGCAGC CGTACGAGCA GCACGCCAAG ATGATCTTCA TGGAGCTGAA TGACGCCTGG
TCGGAGTTTG AGAACCAGGG CTCCCGGCCC CTCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011511733.1
CDS144..3962
Misc Feature(1)258..1103
Misc Feature(2)264..1070
Misc Feature(3)order(279..293,303..305,342..344,348..350,438..440,510..521,528..530,534..536,660..662,666..668,672..677,681..683,714..716,723..725,807..818)
Misc Feature(4)order(279..293,303..305,342..344,348..350,438..440,510..521,528..530,660..662,666..668,672..677,681..683,714..716)
Misc Feature(5)order(291..293,528..530,534..536,660..662,666..668,672..674,723..725,807..818)
Misc Feature(6)order(711..803,807..818)
Misc Feature(7)1851..2267
Misc Feature(8)<3804..3938
Misc Feature(9)order(3849..3857,3891..3893,3900..3905,3912..3917)
Translation

Target ORF information:

RefSeq Version XM_011513431.2
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin G associated kinase (GAK), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011513431.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATGTG
GGGAGTGGCA GAGAGTATGC ATTAAAGAGG CTATTATCCA ATGAAGAGGA AAAGAACAGA
GCCATCATTC AAGAAGTTTG CTTCATGAAA AAGCTTTCCG GCCACCCGAA CATTGTCCAG
TTTTGTTCTG CAGCGTCTAT AGGAAAAGAG GAGTCAGACA CGGGGCAGGC TGAGTTCCTC
TTGCTCACAG AGCTCTGTAA AGGGCAGCTG GTGGAATTTT TGAAGAAAAT GGAATCTCGA
GGCCCCCTTT CGTGCGACAC GGTTCTGAAG ATCTTCTACC AGACGTGCCG CGCCGTGCAG
CACATGCACC GGCAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA GAACTTGTTG
CTTAGTAACC AAGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACGAC CATCTCGCAC
TACCCTGACT ACAGCTGGAG CGCCCAGAGG CGAGCCCTGG TGGAGGAAGA GATCACGAGG
AATACAACAC CAATGTATAG AACACCAGAA ATCATAGACT TGTATTCCAA CTTCCCGATC
GGCGAGAAGC AGGATATCTG GGCCCTGGGC TGCATCTTGT ACCTGCTGTG CTTCCGGCAG
CACCCTTTTG AGGATGGAGC GAAACTTCGA ATAGTCAATG GGAAGTACTC GATCCCCCCG
CACGACACGC AGTACACGGT CTTCCACAGC CTCATCCGCG CCATGCTGCA GGTGAACCCG
GAGGAGCGGC TGTCCATCGC CGAGGTGGTG CACCAGCTGC AGGAGATCGC GGCCGCCCGC
AACGTGAACC CCAAGTCTCC CATCACAGAG CTCCTGGAGC AGAATGGAGG CTACGGGAGC
GCCACACTGT CCCGAGGGCC ACCCCCTCCC GTGGGCCCCG CTGGCAGTGG CTACAGTGGA
GGCCTGGCGC TGGCGGAGTA CGACCAGCCG TATGGCGGCT TCCTGGACAT TCTGCGGGGT
GGGACAGAGC GGCTCTTCAC CAACCTCAAG GACACCTCCT CCAAGGTCAT CCAGTCCGTC
GCTAATTATG CAAAGGGTGA CCTGGACATA TCTTACATCA CATCCAGAAT TGCAGTGATG
TCATTCCCAG CAGAAGGTGT GGAGTCAGCG CTCAAAAACA ACATCGAAGA TGTGCGGTTG
TTCCTGGACT CCAAGCACCC AGGGCACTAT GCCGTCTACA ACCTGTCCCC GAGGACCTAC
CGGCCCTCCA GGTTCCACAA CCGGGTCTCC GAGTGTGGCT GGGCAGCACG GCGGGCCCCA
CACCTGCACA CCCTGTACAA CATCTGCAGG AACATGCACG CCTGGCTGCG GCAGGACCAC
AAGAACGTCT GCGTCGTGCA CTGCATGGAC GGGAGAGCCG CGTCTGCTGT GGCCGTCTGC
TCCTTCCTGT GCTTCTGCCG TCTCTTCAGC ACCGCGGAGG CCGCCGTGTA CATGTTCAGC
ATGAAGCGCT GCCCACCAGG CATCTGGCCA TCCCACAAAA GGTACATCGA GTACATGTGT
GACATGGTGG CGGAGGAGCC CATCACACCC CACAGCAAGC CCATCCTGGT GAGGGCCGTG
GTCATGACAC CCGTGCCGCT GTTCAGCAAG CAGAGGAGCG GCTGCAGGCC CTTCTGCGAG
GTCTACGTGG GGGACGAGCG TGTGGCCAGC ACCTCCCAGG AGTACGACAA GATGCGGGAC
TTTAAGATTG AAGATGGCAA AGCGGTGATT CCCCTGGGCG TCACGGTGCA AGGAGACGTG
CTCATCGTCA TCTATCACGC CCGGTCCACT CTGGGCGGCC GGCTGCAGGC CAAGATGGCA
TCCATGAAGA TGTTCCAGAT TCAGTTCCAC ACGGGGTTTG TGCCTCGGAA CGCCACCACT
GTGAAATTTG CCAAGTATGA CCTGGACGCG TGTGACATTC AAGAAAAATA CCCGGATTTA
TTTCAAGTGA ACCTGGAAGT GGAGGTGGAG CCCAGGGACA GGCCGAGCCG GGAAGCCCCA
CCATGGGAGA ACTCGAGCAT GAGGGGGCTG AACCCCAAAA TCCTGTTTTC CAGCCGGGAG
GAGCAGCAAG ACATTCTGTC TAAGTTTGGG AAGCCGGAGC TTCCCCGGCA GCCTGGCTCC
ACGGCTCAGT ATGATGCTGG GGCAGGGTCC CCGGAAGCCG AACCCACAGA CTCTGACTCA
CCGCCAAGCA GCAGCGCGGA CGCCAGTCGC TTCCTGCACA CGCTGGACTG GCAGGAAGAG
AAGGAGGCAG AGACTGGTGC AGAAAATGCC TCTTCCAAGG AGAGCGAGTC TGCCCTGATG
GAGGACAGAG ACGAGAGTGA GGTGTCAGAT GAAGGGGGAT CCCCGATCTC CAGCGAGGGC
CAGGAACCCA GGGCCGACCC AGAGCCCCCC GGCCTGGCAG CAGGGCTGGT GCAGCAGGAC
TTGGTTTTTG AGGTGGAGAC ACCGGCTGTG CTGCCAGAGC CTGTGCCACA GGAAGACGGG
GTCGACCTCC TGGGCCTGCA CTCCGAGGTG GGCGCAGGGC CAGCTGTACC CCCGCAGGCC
TGCAAGGCCC CCTCCAGCAA CACCGACCTG CTCAGCTGCC TCCTTGGGCC CCCTGAGGCC
GCCTCCCAGG GGCCCCCGGA GGATCTGCTC AGCGAGGACC CGCTGCTCCT GGCAAGCCCG
GCCCCTCCCC TGAGCGTGCA GAGCACCCCA AGAGGAGGGC CCCCTGCCGC TGCTGACCCC
TTTGGCCCGC TTCTGCCGTC TTCAGGCAAC AACTCCCAGC CCTGCTCCAA TCCTGATCTC
TTCGGCGAAT TTCTCAATTC GGACTCTGTG ACCGTCCCAC CATCCTTCCC GTCTGCCCAC
AGTGCTCCGC CCCCATCCTG CAGCGCCGAC TTCCTGCACC TGGGGGATCT GCCAGGAGAG
CCCAGCAAGA TGACAGCCTC GTCCAGCAAC CCAGACCTGC TGGGAGGATG GGCTGCCTGG
ACCGAGACTG CAGCATCGGC AGTGGCCCCC ACGCCAGCCA CAGAAGGCTC ACCAGCTGGA
TTCCCTCCTG GGGGCTTCAT TCCCAAAACG GCCACCACGC CCAAAGGCAG CAGCTCCTGG
CAGACAAGTC GGCCGCCAGC CCAGGGCGCC TCATGGCCCC CTCAGGCCAA GCCGCCCCCC
AAAGCCTGCA CACAGCCAAG GCCTAACTAT GCCTCGAACT TCAGTGTGAT CGGGGCGCGG
GAGGAGCGGG GGGTCCGCGC ACCCAGCTTT GCTCAAAAGC CAAAAGTCTC TGAGAACGAC
TTTGAAGATC TGTTGTCCAA TCAAGGCTTC TCCTCCAGGT CTGACAAGAA AGGGCCAAAG
ACCATTGCAG AGATGAGGAA GCAGGACCTG GCTAAAGACA CGGACCCACT CAAGCTGAAG
CTCCTGGACT GGATTGAGGG CAAGGAGCGG AACATCCGGG CCCTGCTGTC CACGCTGCAC
ACAGTGCTGT GGGACGGGGA GAGCCGCTGG ACGCCCGTGG GCATGGCCGA CCTGGTGGCT
CCGGAGCAAG TGAAGAAGCA CTATCGCCGC GCGGTGCTGG CTGTGCACCC CGACAAGGCT
GCGGGGCAGC CGTACGAGCA GCACGCCAAG ATGATCTTCA TGGAGCTGAA TGACGCCTGG
TCGGAGTTTG AGAACCAGGG CTCCCGGCCC CTCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57910
Clone ID Related Accession (Same CDS sequence) XM_011513432.2
Accession Version XM_011513432.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3804bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product cyclin-G-associated kinase isoform X10
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011513432.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGAAAAAGC TTTCCGGCCA CCCGAACATT GTCCAGTTTT GTTCTGCAGC GTCTATAGGA 
AAAGAGGAGT CAGACACGGG GCAGGCTGAG TTCCTCTTGC TCACAGAGCT CTGTAAAGGG
CAGCTGGTGG AATTTTTGAA GAAAATGGAA TCTCGAGGCC CCCTTTCGTG CGACACGGTT
CTGAAGATCT TCTACCAGAC GTGCCGCGCC GTGCAGCACA TGCACCGGCA GAAGCCGCCC
ATCATCCACA GGGACCTCAA GGTTGAGAAC TTGTTGCTTA GTAACCAAGG GACCATTAAG
CTGTGTGACT TTGGCAGTGC CACGACCATC TCGCACTACC CTGACTACAG CTGGAGCGCC
CAGAGGCGAG CCCTGGTGGA GGAAGAGATC ACGAGGAATA CAACACCAAT GTATAGAACA
CCAGAAATCA TAGACTTGTA TTCCAACTTC CCGATCGGCG AGAAGCAGGA TATCTGGGCC
CTGGGCTGCA TCTTGTACCT GCTGTGCTTC CGGCAGCACC CTTTTGAGGA TGGAGCGAAA
CTTCGAATAG TCAATGGGAA GTACTCGATC CCCCCGCACG ACACGCAGTA CACGGTCTTC
CACAGCCTCA TCCGCGCCAT GCTGCAGGTG AACCCGGAGG AGCGGCTGTC CATCGCCGAG
GTGGTGCACC AGCTGCAGGA GATCGCGGCC GCCCGCAACG TGAACCCCAA GTCTCCCATC
ACAGAGCTCC TGGAGCAGAA TGGAGGCTAC GGGAGCGCCA CACTGTCCCG AGGGCCACCC
CCTCCCGTGG GCCCCGCTGG CAGTGGCTAC AGTGGAGGCC TGGCGCTGGC GGAGTACGAC
CAGCCGTATG GCGGCTTCCT GGACATTCTG CGGGGTGGGA CAGAGCGGCT CTTCACCAAC
CTCAAGGACA CCTCCTCCAA GGTCATCCAG TCCGTCGCTA ATTATGCAAA GGGTGACCTG
GACATATCTT ACATCACATC CAGAATTGCA GTGATGTCAT TCCCAGCAGA AGGTGTGGAG
TCAGCGCTCA AAAACAACAT CGAAGATGTG CGGTTGTTCC TGGACTCCAA GCACCCAGGG
CACTATGCCG TCTACAACCT GTCCCCGAGG ACCTACCGGC CCTCCAGGTT CCACAACCGG
GTCTCCGAGT GTGGCTGGGC AGCACGGCGG GCCCCACACC TGCACACCCT GTACAACATC
TGCAGGAACA TGCACGCCTG GCTGCGGCAG GACCACAAGA ACGTCTGCGT CGTGCACTGC
ATGGACGGGA GAGCCGCGTC TGCTGTGGCC GTCTGCTCCT TCCTGTGCTT CTGCCGTCTC
TTCAGCACCG CGGAGGCCGC CGTGTACATG TTCAGCATGA AGCGCTGCCC ACCAGGCATC
TGGCCATCCC ACAAAAGGTA CATCGAGTAC ATGTGTGACA TGGTGGCGGA GGAGCCCATC
ACACCCCACA GCAAGCCCAT CCTGGTGAGG GCCGTGGTCA TGACACCCGT GCCGCTGTTC
AGCAAGCAGA GGAGCGGCTG CAGGCCCTTC TGCGAGGTCT ACGTGGGGGA CGAGCGTGTG
GCCAGCACCT CCCAGGAGTA CGACAAGATG CGGGACTTTA AGATTGAAGA TGGCAAAGCG
GTGATTCCCC TGGGCGTCAC GGTGCAAGGA GACGTGCTCA TCGTCATCTA TCACGCCCGG
TCCACTCTGG GCGGCCGGCT GCAGGCCAAG ATGGCATCCA TGAAGATGTT CCAGATTCAG
TTCCACACGG GGTTTGTGCC TCGGAACGCC ACCACTGTGA AATTTGCCAA GTATGACCTG
GACGCGTGTG ACATTCAAGA AAAATACCCG GATTTATTTC AAGTGAACCT GGAAGTGGAG
GTGGAGCCCA GGGACAGGCC GAGCCGGGAA GCCCCACCAT GGGAGAACTC GAGCATGAGG
GGGCTGAACC CCAAAATCCT GTTTTCCAGC CGGGAGGAGC AGCAAGACAT TCTGTCTAAG
TTTGGGAAGC CGGAGCTTCC CCGGCAGCCT GGCTCCACGG CTCAGTATGA TGCTGGGGCA
GGGTCCCCGG AAGCCGAACC CACAGACTCT GACTCACCGC CAAGCAGCAG CGCGGACGCC
AGTCGCTTCC TGCACACGCT GGACTGGCAG GAAGAGAAGG AGGCAGAGAC TGGTGCAGAA
AATGCCTCTT CCAAGGAGAG CGAGTCTGCC CTGATGGAGG ACAGAGACGA GAGTGAGGTG
TCAGATGAAG GGGGATCCCC GATCTCCAGC GAGGGCCAGG AACCCAGGGC CGACCCAGAG
CCCCCCGGCC TGGCAGCAGG GCTGGTGCAG CAGGACTTGG TTTTTGAGGT GGAGACACCG
GCTGTGCTGC CAGAGCCTGT GCCACAGGAA GACGGGGTCG ACCTCCTGGG CCTGCACTCC
GAGGTGGGCG CAGGGCCAGC TGTACCCCCG CAGGCCTGCA AGGCCCCCTC CAGCAACACC
GACCTGCTCA GCTGCCTCCT TGGGCCCCCT GAGGCCGCCT CCCAGGGGCC CCCGGAGGAT
CTGCTCAGCG AGGACCCGCT GCTCCTGGCA AGCCCGGCCC CTCCCCTGAG CGTGCAGAGC
ACCCCAAGAG GAGGGCCCCC TGCCGCTGCT GACCCCTTTG GCCCGCTTCT GCCGTCTTCA
GGCAACAACT CCCAGCCCTG CTCCAATCCT GATCTCTTCG GCGAATTTCT CAATTCGGAC
TCTGTGACCG TCCCACCATC CTTCCCGTCT GCCCACAGTG CTCCGCCCCC ATCCTGCAGC
GCCGACTTCC TGCACCTGGG GGATCTGCCA GGAGAGCCCA GCAAGATGAC AGCCTCGTCC
AGCAACCCAG ACCTGCTGGG AGGATGGGCT GCCTGGACCG AGACTGCAGC ATCGGCAGTG
GCCCCCACGC CAGCCACAGA AGGCCCCCTC TTCTCTCCTG GAGGTCAGCC GGCCCCTTGT
GGCTCTCAGG CCAGCTGGAC CAAGTCTCAG AACCCGGACC CATTTGCTGA CCTTGGCGAC
CTCAGCTCCG GCCTCCAAGA CCCCCAAGCC CAGAGCACAG TGAGCCCAAG GGGACAGCGT
GTCTGCACCT GTTCCAGGCG ACTGCCAACT GGCAAGCTAA AACCGGGAGT TGCTGACACT
GGCACTGCTG CCAGCCCCCA CCGGCATTGT GGCTCACCAG CTGGATTCCC TCCTGGGGGC
TTCATTCCCA AAACGGCCAC CACGCCCAAA GGCAGCAGCT CCTGGCAGAC AAGTCGGCCG
CCAGCCCAGG GCGCCTCATG GCCCCCTCAG GCCAAGCCGC CCCCCAAAGC CTGCACACAG
CCAAGGCCTA ACTATGCCTC GAACTTCAGT GTGATCGGGG CGCGGGAGGA GCGGGGGGTC
CGCGCACCCA GCTTTGCTCA AAAGCCAAAA GTCTCTGAGA ACGACTTTGA AGATCTGTTG
TCCAATCAAG GCTTCTCCTC CAGGTCTGAC AAGAAAGGGC CAAAGACCAT TGCAGAGATG
AGGAAGCAGG ACCTGGCTAA AGACACGGAC CCACTCAAGC TGAAGCTCCT GGACTGGATT
GAGGGCAAGG AGCGGAACAT CCGGGCCCTG CTGTCCACGC TGCACACAGT GCTGTGGGAC
GGGGAGAGCC GCTGGACGCC CGTGGGCATG GCCGACCTGG TGGCTCCGGA GCAAGTGAAG
AAGCACTATC GCCGCGCGGT GCTGGCTGTG CACCCCGACA AGGCTGCGGG GCAGCCGTAC
GAGCAGCACG CCAAGATGAT CTTCATGGAG CTGAATGACG CCTGGTCGGA GTTTGAGAAC
CAGGGCTCCC GGCCCCTCTT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011511734.1
CDS379..4182
Misc Feature(1)379..1074
Misc Feature(2)379..1041
Misc Feature(3)1822..2238
Misc Feature(4)<4024..4158
Misc Feature(5)order(4069..4077,4111..4113,4120..4125,4132..4137)
Translation

Target ORF information:

RefSeq Version XM_011513432.2
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin G associated kinase (GAK), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011513432.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGAAAAAGC TTTCCGGCCA CCCGAACATT GTCCAGTTTT GTTCTGCAGC GTCTATAGGA 
AAAGAGGAGT CAGACACGGG GCAGGCTGAG TTCCTCTTGC TCACAGAGCT CTGTAAAGGG
CAGCTGGTGG AATTTTTGAA GAAAATGGAA TCTCGAGGCC CCCTTTCGTG CGACACGGTT
CTGAAGATCT TCTACCAGAC GTGCCGCGCC GTGCAGCACA TGCACCGGCA GAAGCCGCCC
ATCATCCACA GGGACCTCAA GGTTGAGAAC TTGTTGCTTA GTAACCAAGG GACCATTAAG
CTGTGTGACT TTGGCAGTGC CACGACCATC TCGCACTACC CTGACTACAG CTGGAGCGCC
CAGAGGCGAG CCCTGGTGGA GGAAGAGATC ACGAGGAATA CAACACCAAT GTATAGAACA
CCAGAAATCA TAGACTTGTA TTCCAACTTC CCGATCGGCG AGAAGCAGGA TATCTGGGCC
CTGGGCTGCA TCTTGTACCT GCTGTGCTTC CGGCAGCACC CTTTTGAGGA TGGAGCGAAA
CTTCGAATAG TCAATGGGAA GTACTCGATC CCCCCGCACG ACACGCAGTA CACGGTCTTC
CACAGCCTCA TCCGCGCCAT GCTGCAGGTG AACCCGGAGG AGCGGCTGTC CATCGCCGAG
GTGGTGCACC AGCTGCAGGA GATCGCGGCC GCCCGCAACG TGAACCCCAA GTCTCCCATC
ACAGAGCTCC TGGAGCAGAA TGGAGGCTAC GGGAGCGCCA CACTGTCCCG AGGGCCACCC
CCTCCCGTGG GCCCCGCTGG CAGTGGCTAC AGTGGAGGCC TGGCGCTGGC GGAGTACGAC
CAGCCGTATG GCGGCTTCCT GGACATTCTG CGGGGTGGGA CAGAGCGGCT CTTCACCAAC
CTCAAGGACA CCTCCTCCAA GGTCATCCAG TCCGTCGCTA ATTATGCAAA GGGTGACCTG
GACATATCTT ACATCACATC CAGAATTGCA GTGATGTCAT TCCCAGCAGA AGGTGTGGAG
TCAGCGCTCA AAAACAACAT CGAAGATGTG CGGTTGTTCC TGGACTCCAA GCACCCAGGG
CACTATGCCG TCTACAACCT GTCCCCGAGG ACCTACCGGC CCTCCAGGTT CCACAACCGG
GTCTCCGAGT GTGGCTGGGC AGCACGGCGG GCCCCACACC TGCACACCCT GTACAACATC
TGCAGGAACA TGCACGCCTG GCTGCGGCAG GACCACAAGA ACGTCTGCGT CGTGCACTGC
ATGGACGGGA GAGCCGCGTC TGCTGTGGCC GTCTGCTCCT TCCTGTGCTT CTGCCGTCTC
TTCAGCACCG CGGAGGCCGC CGTGTACATG TTCAGCATGA AGCGCTGCCC ACCAGGCATC
TGGCCATCCC ACAAAAGGTA CATCGAGTAC ATGTGTGACA TGGTGGCGGA GGAGCCCATC
ACACCCCACA GCAAGCCCAT CCTGGTGAGG GCCGTGGTCA TGACACCCGT GCCGCTGTTC
AGCAAGCAGA GGAGCGGCTG CAGGCCCTTC TGCGAGGTCT ACGTGGGGGA CGAGCGTGTG
GCCAGCACCT CCCAGGAGTA CGACAAGATG CGGGACTTTA AGATTGAAGA TGGCAAAGCG
GTGATTCCCC TGGGCGTCAC GGTGCAAGGA GACGTGCTCA TCGTCATCTA TCACGCCCGG
TCCACTCTGG GCGGCCGGCT GCAGGCCAAG ATGGCATCCA TGAAGATGTT CCAGATTCAG
TTCCACACGG GGTTTGTGCC TCGGAACGCC ACCACTGTGA AATTTGCCAA GTATGACCTG
GACGCGTGTG ACATTCAAGA AAAATACCCG GATTTATTTC AAGTGAACCT GGAAGTGGAG
GTGGAGCCCA GGGACAGGCC GAGCCGGGAA GCCCCACCAT GGGAGAACTC GAGCATGAGG
GGGCTGAACC CCAAAATCCT GTTTTCCAGC CGGGAGGAGC AGCAAGACAT TCTGTCTAAG
TTTGGGAAGC CGGAGCTTCC CCGGCAGCCT GGCTCCACGG CTCAGTATGA TGCTGGGGCA
GGGTCCCCGG AAGCCGAACC CACAGACTCT GACTCACCGC CAAGCAGCAG CGCGGACGCC
AGTCGCTTCC TGCACACGCT GGACTGGCAG GAAGAGAAGG AGGCAGAGAC TGGTGCAGAA
AATGCCTCTT CCAAGGAGAG CGAGTCTGCC CTGATGGAGG ACAGAGACGA GAGTGAGGTG
TCAGATGAAG GGGGATCCCC GATCTCCAGC GAGGGCCAGG AACCCAGGGC CGACCCAGAG
CCCCCCGGCC TGGCAGCAGG GCTGGTGCAG CAGGACTTGG TTTTTGAGGT GGAGACACCG
GCTGTGCTGC CAGAGCCTGT GCCACAGGAA GACGGGGTCG ACCTCCTGGG CCTGCACTCC
GAGGTGGGCG CAGGGCCAGC TGTACCCCCG CAGGCCTGCA AGGCCCCCTC CAGCAACACC
GACCTGCTCA GCTGCCTCCT TGGGCCCCCT GAGGCCGCCT CCCAGGGGCC CCCGGAGGAT
CTGCTCAGCG AGGACCCGCT GCTCCTGGCA AGCCCGGCCC CTCCCCTGAG CGTGCAGAGC
ACCCCAAGAG GAGGGCCCCC TGCCGCTGCT GACCCCTTTG GCCCGCTTCT GCCGTCTTCA
GGCAACAACT CCCAGCCCTG CTCCAATCCT GATCTCTTCG GCGAATTTCT CAATTCGGAC
TCTGTGACCG TCCCACCATC CTTCCCGTCT GCCCACAGTG CTCCGCCCCC ATCCTGCAGC
GCCGACTTCC TGCACCTGGG GGATCTGCCA GGAGAGCCCA GCAAGATGAC AGCCTCGTCC
AGCAACCCAG ACCTGCTGGG AGGATGGGCT GCCTGGACCG AGACTGCAGC ATCGGCAGTG
GCCCCCACGC CAGCCACAGA AGGCCCCCTC TTCTCTCCTG GAGGTCAGCC GGCCCCTTGT
GGCTCTCAGG CCAGCTGGAC CAAGTCTCAG AACCCGGACC CATTTGCTGA CCTTGGCGAC
CTCAGCTCCG GCCTCCAAGA CCCCCAAGCC CAGAGCACAG TGAGCCCAAG GGGACAGCGT
GTCTGCACCT GTTCCAGGCG ACTGCCAACT GGCAAGCTAA AACCGGGAGT TGCTGACACT
GGCACTGCTG CCAGCCCCCA CCGGCATTGT GGCTCACCAG CTGGATTCCC TCCTGGGGGC
TTCATTCCCA AAACGGCCAC CACGCCCAAA GGCAGCAGCT CCTGGCAGAC AAGTCGGCCG
CCAGCCCAGG GCGCCTCATG GCCCCCTCAG GCCAAGCCGC CCCCCAAAGC CTGCACACAG
CCAAGGCCTA ACTATGCCTC GAACTTCAGT GTGATCGGGG CGCGGGAGGA GCGGGGGGTC
CGCGCACCCA GCTTTGCTCA AAAGCCAAAA GTCTCTGAGA ACGACTTTGA AGATCTGTTG
TCCAATCAAG GCTTCTCCTC CAGGTCTGAC AAGAAAGGGC CAAAGACCAT TGCAGAGATG
AGGAAGCAGG ACCTGGCTAA AGACACGGAC CCACTCAAGC TGAAGCTCCT GGACTGGATT
GAGGGCAAGG AGCGGAACAT CCGGGCCCTG CTGTCCACGC TGCACACAGT GCTGTGGGAC
GGGGAGAGCC GCTGGACGCC CGTGGGCATG GCCGACCTGG TGGCTCCGGA GCAAGTGAAG
AAGCACTATC GCCGCGCGGT GCTGGCTGTG CACCCCGACA AGGCTGCGGG GCAGCCGTAC
GAGCAGCACG CCAAGATGAT CTTCATGGAG CTGAATGACG CCTGGTCGGA GTTTGAGAAC
CAGGGCTCCC GGCCCCTCTT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57912
Clone ID Related Accession (Same CDS sequence) XM_017007993.1 , XM_011513434.2
Accession Version XM_017007993.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3660bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product cyclin-G-associated kinase isoform X13
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGAATCTC GAGGCCCCCT TTCGTGCGAC ACGGTTCTGA AGATCTTCTA CCAGACGTGC 
CGCGCCGTGC AGCACATGCA CCGGCAGAAG CCGCCCATCA TCCACAGGGA CCTCAAGGTT
GAGAACTTGT TGCTTAGTAA CCAAGGGACC ATTAAGCTGT GTGACTTTGG CAGTGCCACG
ACCATCTCGC ACTACCCTGA CTACAGCTGG AGCGCCCAGA GGCGAGCCCT GGTGGAGGAA
GAGATCACGA GGAATACAAC ACCAATGTAT AGAACACCAG AAATCATAGA CTTGTATTCC
AACTTCCCGA TCGGCGAGAA GCAGGATATC TGGGCCCTGG GCTGCATCTT GTACCTGCTG
TGCTTCCGGC AGCACCCTTT TGAGGATGGA GCGAAACTTC GAATAGTCAA TGGGAAGTAC
TCGATCCCCC CGCACGACAC GCAGTACACG GTCTTCCACA GCCTCATCCG CGCCATGCTG
CAGGTGAACC CGGAGGAGCG GCTGTCCATC GCCGAGGTGG TGCACCAGCT GCAGGAGATC
GCGGCCGCCC GCAACGTGAA CCCCAAGTCT CCCATCACAG AGCTCCTGGA GCAGAATGGA
GGCTACGGGA GCGCCACACT GTCCCGAGGG CCACCCCCTC CCGTGGGCCC CGCTGGCAGT
GGCTACAGTG GAGGCCTGGC GCTGGCGGAG TACGACCAGC CGTATGGCGG CTTCCTGGAC
ATTCTGCGGG GTGGGACAGA GCGGCTCTTC ACCAACCTCA AGGACACCTC CTCCAAGGTC
ATCCAGTCCG TCGCTAATTA TGCAAAGGGT GACCTGGACA TATCTTACAT CACATCCAGA
ATTGCAGTGA TGTCATTCCC AGCAGAAGGT GTGGAGTCAG CGCTCAAAAA CAACATCGAA
GATGTGCGGT TGTTCCTGGA CTCCAAGCAC CCAGGGCACT ATGCCGTCTA CAACCTGTCC
CCGAGGACCT ACCGGCCCTC CAGGTTCCAC AACCGGGTCT CCGAGTGTGG CTGGGCAGCA
CGGCGGGCCC CACACCTGCA CACCCTGTAC AACATCTGCA GGAACATGCA CGCCTGGCTG
CGGCAGGACC ACAAGAACGT CTGCGTCGTG CACTGCATGG ACGGGAGAGC CGCGTCTGCT
GTGGCCGTCT GCTCCTTCCT GTGCTTCTGC CGTCTCTTCA GCACCGCGGA GGCCGCCGTG
TACATGTTCA GCATGAAGCG CTGCCCACCA GGCATCTGGC CATCCCACAA AAGGTACATC
GAGTACATGT GTGACATGGT GGCGGAGGAG CCCATCACAC CCCACAGCAA GCCCATCCTG
GTGAGGGCCG TGGTCATGAC ACCCGTGCCG CTGTTCAGCA AGCAGAGGAG CGGCTGCAGG
CCCTTCTGCG AGGTCTACGT GGGGGACGAG CGTGTGGCCA GCACCTCCCA GGAGTACGAC
AAGATGCGGG ACTTTAAGAT TGAAGATGGC AAAGCGGTGA TTCCCCTGGG CGTCACGGTG
CAAGGAGACG TGCTCATCGT CATCTATCAC GCCCGGTCCA CTCTGGGCGG CCGGCTGCAG
GCCAAGATGG CATCCATGAA GATGTTCCAG ATTCAGTTCC ACACGGGGTT TGTGCCTCGG
AACGCCACCA CTGTGAAATT TGCCAAGTAT GACCTGGACG CGTGTGACAT TCAAGAAAAA
TACCCGGATT TATTTCAAGT GAACCTGGAA GTGGAGGTGG AGCCCAGGGA CAGGCCGAGC
CGGGAAGCCC CACCATGGGA GAACTCGAGC ATGAGGGGGC TGAACCCCAA AATCCTGTTT
TCCAGCCGGG AGGAGCAGCA AGACATTCTG TCTAAGTTTG GGAAGCCGGA GCTTCCCCGG
CAGCCTGGCT CCACGGCTCA GTATGATGCT GGGGCAGGGT CCCCGGAAGC CGAACCCACA
GACTCTGACT CACCGCCAAG CAGCAGCGCG GACGCCAGTC GCTTCCTGCA CACGCTGGAC
TGGCAGGAAG AGAAGGAGGC AGAGACTGGT GCAGAAAATG CCTCTTCCAA GGAGAGCGAG
TCTGCCCTGA TGGAGGACAG AGACGAGAGT GAGGTGTCAG ATGAAGGGGG ATCCCCGATC
TCCAGCGAGG GCCAGGAACC CAGGGCCGAC CCAGAGCCCC CCGGCCTGGC AGCAGGGCTG
GTGCAGCAGG ACTTGGTTTT TGAGGTGGAG ACACCGGCTG TGCTGCCAGA GCCTGTGCCA
CAGGAAGACG GGGTCGACCT CCTGGGCCTG CACTCCGAGG TGGGCGCAGG GCCAGCTGTA
CCCCCGCAGG CCTGCAAGGC CCCCTCCAGC AACACCGACC TGCTCAGCTG CCTCCTTGGG
CCCCCTGAGG CCGCCTCCCA GGGGCCCCCG GAGGATCTGC TCAGCGAGGA CCCGCTGCTC
CTGGCAAGCC CGGCCCCTCC CCTGAGCGTG CAGAGCACCC CAAGAGGAGG GCCCCCTGCC
GCTGCTGACC CCTTTGGCCC GCTTCTGCCG TCTTCAGGCA ACAACTCCCA GCCCTGCTCC
AATCCTGATC TCTTCGGCGA ATTTCTCAAT TCGGACTCTG TGACCGTCCC ACCATCCTTC
CCGTCTGCCC ACAGTGCTCC GCCCCCATCC TGCAGCGCCG ACTTCCTGCA CCTGGGGGAT
CTGCCAGGAG AGCCCAGCAA GATGACAGCC TCGTCCAGCA ACCCAGACCT GCTGGGAGGA
TGGGCTGCCT GGACCGAGAC TGCAGCATCG GCAGTGGCCC CCACGCCAGC CACAGAAGGC
CCCCTCTTCT CTCCTGGAGG TCAGCCGGCC CCTTGTGGCT CTCAGGCCAG CTGGACCAAG
TCTCAGAACC CGGACCCATT TGCTGACCTT GGCGACCTCA GCTCCGGCCT CCAAGACCCC
CAAGCCCAGA GCACAGTGAG CCCAAGGGGA CAGCGTGTCT GCACCTGTTC CAGGCGACTG
CCAACTGGCA AGCTAAAACC GGGAGTTGCT GACACTGGCA CTGCTGCCAG CCCCCACCGG
CATTGTGGCT CACCAGCTGG ATTCCCTCCT GGGGGCTTCA TTCCCAAAAC GGCCACCACG
CCCAAAGGCA GCAGCTCCTG GCAGACAAGT CGGCCGCCAG CCCAGGGCGC CTCATGGCCC
CCTCAGGCCA AGCCGCCCCC CAAAGCCTGC ACACAGCCAA GGCCTAACTA TGCCTCGAAC
TTCAGTGTGA TCGGGGCGCG GGAGGAGCGG GGGGTCCGCG CACCCAGCTT TGCTCAAAAG
CCAAAAGTCT CTGAGAACGA CTTTGAAGAT CTGTTGTCCA ATCAAGGCTT CTCCTCCAGG
TCTGACAAGA AAGGGCCAAA GACCATTGCA GAGATGAGGA AGCAGGACCT GGCTAAAGAC
ACGGACCCAC TCAAGCTGAA GCTCCTGGAC TGGATTGAGG GCAAGGAGCG GAACATCCGG
GCCCTGCTGT CCACGCTGCA CACAGTGCTG TGGGACGGGG AGAGCCGCTG GACGCCCGTG
GGCATGGCCG ACCTGGTGGC TCCGGAGCAA GTGAAGAAGC ACTATCGCCG CGCGGTGCTG
GCTGTGCACC CCGACAAGGC TGCGGGGCAG CCGTACGAGC AGCACGCCAA GATGATCTTC
ATGGAGCTGA ATGACGCCTG GTCGGAGTTT GAGAACCAGG GCTCCCGGCC CCTCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016863482.1
CDS301..3960
Misc Feature(1)<301..852
Misc Feature(2)<301..819
Misc Feature(3)1600..2016
Misc Feature(4)<3802..3936
Misc Feature(5)order(3847..3855,3889..3891,3898..3903,3910..3915)
Translation

Target ORF information:

RefSeq Version XM_017007993.1
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin G associated kinase (GAK), transcript variant X14, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017007993.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGAATCTC GAGGCCCCCT TTCGTGCGAC ACGGTTCTGA AGATCTTCTA CCAGACGTGC 
CGCGCCGTGC AGCACATGCA CCGGCAGAAG CCGCCCATCA TCCACAGGGA CCTCAAGGTT
GAGAACTTGT TGCTTAGTAA CCAAGGGACC ATTAAGCTGT GTGACTTTGG CAGTGCCACG
ACCATCTCGC ACTACCCTGA CTACAGCTGG AGCGCCCAGA GGCGAGCCCT GGTGGAGGAA
GAGATCACGA GGAATACAAC ACCAATGTAT AGAACACCAG AAATCATAGA CTTGTATTCC
AACTTCCCGA TCGGCGAGAA GCAGGATATC TGGGCCCTGG GCTGCATCTT GTACCTGCTG
TGCTTCCGGC AGCACCCTTT TGAGGATGGA GCGAAACTTC GAATAGTCAA TGGGAAGTAC
TCGATCCCCC CGCACGACAC GCAGTACACG GTCTTCCACA GCCTCATCCG CGCCATGCTG
CAGGTGAACC CGGAGGAGCG GCTGTCCATC GCCGAGGTGG TGCACCAGCT GCAGGAGATC
GCGGCCGCCC GCAACGTGAA CCCCAAGTCT CCCATCACAG AGCTCCTGGA GCAGAATGGA
GGCTACGGGA GCGCCACACT GTCCCGAGGG CCACCCCCTC CCGTGGGCCC CGCTGGCAGT
GGCTACAGTG GAGGCCTGGC GCTGGCGGAG TACGACCAGC CGTATGGCGG CTTCCTGGAC
ATTCTGCGGG GTGGGACAGA GCGGCTCTTC ACCAACCTCA AGGACACCTC CTCCAAGGTC
ATCCAGTCCG TCGCTAATTA TGCAAAGGGT GACCTGGACA TATCTTACAT CACATCCAGA
ATTGCAGTGA TGTCATTCCC AGCAGAAGGT GTGGAGTCAG CGCTCAAAAA CAACATCGAA
GATGTGCGGT TGTTCCTGGA CTCCAAGCAC CCAGGGCACT ATGCCGTCTA CAACCTGTCC
CCGAGGACCT ACCGGCCCTC CAGGTTCCAC AACCGGGTCT CCGAGTGTGG CTGGGCAGCA
CGGCGGGCCC CACACCTGCA CACCCTGTAC AACATCTGCA GGAACATGCA CGCCTGGCTG
CGGCAGGACC ACAAGAACGT CTGCGTCGTG CACTGCATGG ACGGGAGAGC CGCGTCTGCT
GTGGCCGTCT GCTCCTTCCT GTGCTTCTGC CGTCTCTTCA GCACCGCGGA GGCCGCCGTG
TACATGTTCA GCATGAAGCG CTGCCCACCA GGCATCTGGC CATCCCACAA AAGGTACATC
GAGTACATGT GTGACATGGT GGCGGAGGAG CCCATCACAC CCCACAGCAA GCCCATCCTG
GTGAGGGCCG TGGTCATGAC ACCCGTGCCG CTGTTCAGCA AGCAGAGGAG CGGCTGCAGG
CCCTTCTGCG AGGTCTACGT GGGGGACGAG CGTGTGGCCA GCACCTCCCA GGAGTACGAC
AAGATGCGGG ACTTTAAGAT TGAAGATGGC AAAGCGGTGA TTCCCCTGGG CGTCACGGTG
CAAGGAGACG TGCTCATCGT CATCTATCAC GCCCGGTCCA CTCTGGGCGG CCGGCTGCAG
GCCAAGATGG CATCCATGAA GATGTTCCAG ATTCAGTTCC ACACGGGGTT TGTGCCTCGG
AACGCCACCA CTGTGAAATT TGCCAAGTAT GACCTGGACG CGTGTGACAT TCAAGAAAAA
TACCCGGATT TATTTCAAGT GAACCTGGAA GTGGAGGTGG AGCCCAGGGA CAGGCCGAGC
CGGGAAGCCC CACCATGGGA GAACTCGAGC ATGAGGGGGC TGAACCCCAA AATCCTGTTT
TCCAGCCGGG AGGAGCAGCA AGACATTCTG TCTAAGTTTG GGAAGCCGGA GCTTCCCCGG
CAGCCTGGCT CCACGGCTCA GTATGATGCT GGGGCAGGGT CCCCGGAAGC CGAACCCACA
GACTCTGACT CACCGCCAAG CAGCAGCGCG GACGCCAGTC GCTTCCTGCA CACGCTGGAC
TGGCAGGAAG AGAAGGAGGC AGAGACTGGT GCAGAAAATG CCTCTTCCAA GGAGAGCGAG
TCTGCCCTGA TGGAGGACAG AGACGAGAGT GAGGTGTCAG ATGAAGGGGG ATCCCCGATC
TCCAGCGAGG GCCAGGAACC CAGGGCCGAC CCAGAGCCCC CCGGCCTGGC AGCAGGGCTG
GTGCAGCAGG ACTTGGTTTT TGAGGTGGAG ACACCGGCTG TGCTGCCAGA GCCTGTGCCA
CAGGAAGACG GGGTCGACCT CCTGGGCCTG CACTCCGAGG TGGGCGCAGG GCCAGCTGTA
CCCCCGCAGG CCTGCAAGGC CCCCTCCAGC AACACCGACC TGCTCAGCTG CCTCCTTGGG
CCCCCTGAGG CCGCCTCCCA GGGGCCCCCG GAGGATCTGC TCAGCGAGGA CCCGCTGCTC
CTGGCAAGCC CGGCCCCTCC CCTGAGCGTG CAGAGCACCC CAAGAGGAGG GCCCCCTGCC
GCTGCTGACC CCTTTGGCCC GCTTCTGCCG TCTTCAGGCA ACAACTCCCA GCCCTGCTCC
AATCCTGATC TCTTCGGCGA ATTTCTCAAT TCGGACTCTG TGACCGTCCC ACCATCCTTC
CCGTCTGCCC ACAGTGCTCC GCCCCCATCC TGCAGCGCCG ACTTCCTGCA CCTGGGGGAT
CTGCCAGGAG AGCCCAGCAA GATGACAGCC TCGTCCAGCA ACCCAGACCT GCTGGGAGGA
TGGGCTGCCT GGACCGAGAC TGCAGCATCG GCAGTGGCCC CCACGCCAGC CACAGAAGGC
CCCCTCTTCT CTCCTGGAGG TCAGCCGGCC CCTTGTGGCT CTCAGGCCAG CTGGACCAAG
TCTCAGAACC CGGACCCATT TGCTGACCTT GGCGACCTCA GCTCCGGCCT CCAAGACCCC
CAAGCCCAGA GCACAGTGAG CCCAAGGGGA CAGCGTGTCT GCACCTGTTC CAGGCGACTG
CCAACTGGCA AGCTAAAACC GGGAGTTGCT GACACTGGCA CTGCTGCCAG CCCCCACCGG
CATTGTGGCT CACCAGCTGG ATTCCCTCCT GGGGGCTTCA TTCCCAAAAC GGCCACCACG
CCCAAAGGCA GCAGCTCCTG GCAGACAAGT CGGCCGCCAG CCCAGGGCGC CTCATGGCCC
CCTCAGGCCA AGCCGCCCCC CAAAGCCTGC ACACAGCCAA GGCCTAACTA TGCCTCGAAC
TTCAGTGTGA TCGGGGCGCG GGAGGAGCGG GGGGTCCGCG CACCCAGCTT TGCTCAAAAG
CCAAAAGTCT CTGAGAACGA CTTTGAAGAT CTGTTGTCCA ATCAAGGCTT CTCCTCCAGG
TCTGACAAGA AAGGGCCAAA GACCATTGCA GAGATGAGGA AGCAGGACCT GGCTAAAGAC
ACGGACCCAC TCAAGCTGAA GCTCCTGGAC TGGATTGAGG GCAAGGAGCG GAACATCCGG
GCCCTGCTGT CCACGCTGCA CACAGTGCTG TGGGACGGGG AGAGCCGCTG GACGCCCGTG
GGCATGGCCG ACCTGGTGGC TCCGGAGCAA GTGAAGAAGC ACTATCGCCG CGCGGTGCTG
GCTGTGCACC CCGACAAGGC TGCGGGGCAG CCGTACGAGC AGCACGCCAA GATGATCTTC
ATGGAGCTGA ATGACGCCTG GTCGGAGTTT GAGAACCAGG GCTCCCGGCC CCTCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57912
Clone ID Related Accession (Same CDS sequence) XM_017007993.1 , XM_011513434.2
Accession Version XM_011513434.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3660bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product cyclin-G-associated kinase isoform X13
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011513434.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGAATCTC GAGGCCCCCT TTCGTGCGAC ACGGTTCTGA AGATCTTCTA CCAGACGTGC 
CGCGCCGTGC AGCACATGCA CCGGCAGAAG CCGCCCATCA TCCACAGGGA CCTCAAGGTT
GAGAACTTGT TGCTTAGTAA CCAAGGGACC ATTAAGCTGT GTGACTTTGG CAGTGCCACG
ACCATCTCGC ACTACCCTGA CTACAGCTGG AGCGCCCAGA GGCGAGCCCT GGTGGAGGAA
GAGATCACGA GGAATACAAC ACCAATGTAT AGAACACCAG AAATCATAGA CTTGTATTCC
AACTTCCCGA TCGGCGAGAA GCAGGATATC TGGGCCCTGG GCTGCATCTT GTACCTGCTG
TGCTTCCGGC AGCACCCTTT TGAGGATGGA GCGAAACTTC GAATAGTCAA TGGGAAGTAC
TCGATCCCCC CGCACGACAC GCAGTACACG GTCTTCCACA GCCTCATCCG CGCCATGCTG
CAGGTGAACC CGGAGGAGCG GCTGTCCATC GCCGAGGTGG TGCACCAGCT GCAGGAGATC
GCGGCCGCCC GCAACGTGAA CCCCAAGTCT CCCATCACAG AGCTCCTGGA GCAGAATGGA
GGCTACGGGA GCGCCACACT GTCCCGAGGG CCACCCCCTC CCGTGGGCCC CGCTGGCAGT
GGCTACAGTG GAGGCCTGGC GCTGGCGGAG TACGACCAGC CGTATGGCGG CTTCCTGGAC
ATTCTGCGGG GTGGGACAGA GCGGCTCTTC ACCAACCTCA AGGACACCTC CTCCAAGGTC
ATCCAGTCCG TCGCTAATTA TGCAAAGGGT GACCTGGACA TATCTTACAT CACATCCAGA
ATTGCAGTGA TGTCATTCCC AGCAGAAGGT GTGGAGTCAG CGCTCAAAAA CAACATCGAA
GATGTGCGGT TGTTCCTGGA CTCCAAGCAC CCAGGGCACT ATGCCGTCTA CAACCTGTCC
CCGAGGACCT ACCGGCCCTC CAGGTTCCAC AACCGGGTCT CCGAGTGTGG CTGGGCAGCA
CGGCGGGCCC CACACCTGCA CACCCTGTAC AACATCTGCA GGAACATGCA CGCCTGGCTG
CGGCAGGACC ACAAGAACGT CTGCGTCGTG CACTGCATGG ACGGGAGAGC CGCGTCTGCT
GTGGCCGTCT GCTCCTTCCT GTGCTTCTGC CGTCTCTTCA GCACCGCGGA GGCCGCCGTG
TACATGTTCA GCATGAAGCG CTGCCCACCA GGCATCTGGC CATCCCACAA AAGGTACATC
GAGTACATGT GTGACATGGT GGCGGAGGAG CCCATCACAC CCCACAGCAA GCCCATCCTG
GTGAGGGCCG TGGTCATGAC ACCCGTGCCG CTGTTCAGCA AGCAGAGGAG CGGCTGCAGG
CCCTTCTGCG AGGTCTACGT GGGGGACGAG CGTGTGGCCA GCACCTCCCA GGAGTACGAC
AAGATGCGGG ACTTTAAGAT TGAAGATGGC AAAGCGGTGA TTCCCCTGGG CGTCACGGTG
CAAGGAGACG TGCTCATCGT CATCTATCAC GCCCGGTCCA CTCTGGGCGG CCGGCTGCAG
GCCAAGATGG CATCCATGAA GATGTTCCAG ATTCAGTTCC ACACGGGGTT TGTGCCTCGG
AACGCCACCA CTGTGAAATT TGCCAAGTAT GACCTGGACG CGTGTGACAT TCAAGAAAAA
TACCCGGATT TATTTCAAGT GAACCTGGAA GTGGAGGTGG AGCCCAGGGA CAGGCCGAGC
CGGGAAGCCC CACCATGGGA GAACTCGAGC ATGAGGGGGC TGAACCCCAA AATCCTGTTT
TCCAGCCGGG AGGAGCAGCA AGACATTCTG TCTAAGTTTG GGAAGCCGGA GCTTCCCCGG
CAGCCTGGCT CCACGGCTCA GTATGATGCT GGGGCAGGGT CCCCGGAAGC CGAACCCACA
GACTCTGACT CACCGCCAAG CAGCAGCGCG GACGCCAGTC GCTTCCTGCA CACGCTGGAC
TGGCAGGAAG AGAAGGAGGC AGAGACTGGT GCAGAAAATG CCTCTTCCAA GGAGAGCGAG
TCTGCCCTGA TGGAGGACAG AGACGAGAGT GAGGTGTCAG ATGAAGGGGG ATCCCCGATC
TCCAGCGAGG GCCAGGAACC CAGGGCCGAC CCAGAGCCCC CCGGCCTGGC AGCAGGGCTG
GTGCAGCAGG ACTTGGTTTT TGAGGTGGAG ACACCGGCTG TGCTGCCAGA GCCTGTGCCA
CAGGAAGACG GGGTCGACCT CCTGGGCCTG CACTCCGAGG TGGGCGCAGG GCCAGCTGTA
CCCCCGCAGG CCTGCAAGGC CCCCTCCAGC AACACCGACC TGCTCAGCTG CCTCCTTGGG
CCCCCTGAGG CCGCCTCCCA GGGGCCCCCG GAGGATCTGC TCAGCGAGGA CCCGCTGCTC
CTGGCAAGCC CGGCCCCTCC CCTGAGCGTG CAGAGCACCC CAAGAGGAGG GCCCCCTGCC
GCTGCTGACC CCTTTGGCCC GCTTCTGCCG TCTTCAGGCA ACAACTCCCA GCCCTGCTCC
AATCCTGATC TCTTCGGCGA ATTTCTCAAT TCGGACTCTG TGACCGTCCC ACCATCCTTC
CCGTCTGCCC ACAGTGCTCC GCCCCCATCC TGCAGCGCCG ACTTCCTGCA CCTGGGGGAT
CTGCCAGGAG AGCCCAGCAA GATGACAGCC TCGTCCAGCA ACCCAGACCT GCTGGGAGGA
TGGGCTGCCT GGACCGAGAC TGCAGCATCG GCAGTGGCCC CCACGCCAGC CACAGAAGGC
CCCCTCTTCT CTCCTGGAGG TCAGCCGGCC CCTTGTGGCT CTCAGGCCAG CTGGACCAAG
TCTCAGAACC CGGACCCATT TGCTGACCTT GGCGACCTCA GCTCCGGCCT CCAAGACCCC
CAAGCCCAGA GCACAGTGAG CCCAAGGGGA CAGCGTGTCT GCACCTGTTC CAGGCGACTG
CCAACTGGCA AGCTAAAACC GGGAGTTGCT GACACTGGCA CTGCTGCCAG CCCCCACCGG
CATTGTGGCT CACCAGCTGG ATTCCCTCCT GGGGGCTTCA TTCCCAAAAC GGCCACCACG
CCCAAAGGCA GCAGCTCCTG GCAGACAAGT CGGCCGCCAG CCCAGGGCGC CTCATGGCCC
CCTCAGGCCA AGCCGCCCCC CAAAGCCTGC ACACAGCCAA GGCCTAACTA TGCCTCGAAC
TTCAGTGTGA TCGGGGCGCG GGAGGAGCGG GGGGTCCGCG CACCCAGCTT TGCTCAAAAG
CCAAAAGTCT CTGAGAACGA CTTTGAAGAT CTGTTGTCCA ATCAAGGCTT CTCCTCCAGG
TCTGACAAGA AAGGGCCAAA GACCATTGCA GAGATGAGGA AGCAGGACCT GGCTAAAGAC
ACGGACCCAC TCAAGCTGAA GCTCCTGGAC TGGATTGAGG GCAAGGAGCG GAACATCCGG
GCCCTGCTGT CCACGCTGCA CACAGTGCTG TGGGACGGGG AGAGCCGCTG GACGCCCGTG
GGCATGGCCG ACCTGGTGGC TCCGGAGCAA GTGAAGAAGC ACTATCGCCG CGCGGTGCTG
GCTGTGCACC CCGACAAGGC TGCGGGGCAG CCGTACGAGC AGCACGCCAA GATGATCTTC
ATGGAGCTGA ATGACGCCTG GTCGGAGTTT GAGAACCAGG GCTCCCGGCC CCTCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011511736.1
CDS364..4023
Misc Feature(1)<364..915
Misc Feature(2)<364..882
Misc Feature(3)1663..2079
Misc Feature(4)<3865..3999
Misc Feature(5)order(3910..3918,3952..3954,3961..3966,3973..3978)
Translation

Target ORF information:

RefSeq Version XM_011513434.2
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin G associated kinase (GAK), transcript variant X13, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011513434.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGAATCTC GAGGCCCCCT TTCGTGCGAC ACGGTTCTGA AGATCTTCTA CCAGACGTGC 
CGCGCCGTGC AGCACATGCA CCGGCAGAAG CCGCCCATCA TCCACAGGGA CCTCAAGGTT
GAGAACTTGT TGCTTAGTAA CCAAGGGACC ATTAAGCTGT GTGACTTTGG CAGTGCCACG
ACCATCTCGC ACTACCCTGA CTACAGCTGG AGCGCCCAGA GGCGAGCCCT GGTGGAGGAA
GAGATCACGA GGAATACAAC ACCAATGTAT AGAACACCAG AAATCATAGA CTTGTATTCC
AACTTCCCGA TCGGCGAGAA GCAGGATATC TGGGCCCTGG GCTGCATCTT GTACCTGCTG
TGCTTCCGGC AGCACCCTTT TGAGGATGGA GCGAAACTTC GAATAGTCAA TGGGAAGTAC
TCGATCCCCC CGCACGACAC GCAGTACACG GTCTTCCACA GCCTCATCCG CGCCATGCTG
CAGGTGAACC CGGAGGAGCG GCTGTCCATC GCCGAGGTGG TGCACCAGCT GCAGGAGATC
GCGGCCGCCC GCAACGTGAA CCCCAAGTCT CCCATCACAG AGCTCCTGGA GCAGAATGGA
GGCTACGGGA GCGCCACACT GTCCCGAGGG CCACCCCCTC CCGTGGGCCC CGCTGGCAGT
GGCTACAGTG GAGGCCTGGC GCTGGCGGAG TACGACCAGC CGTATGGCGG CTTCCTGGAC
ATTCTGCGGG GTGGGACAGA GCGGCTCTTC ACCAACCTCA AGGACACCTC CTCCAAGGTC
ATCCAGTCCG TCGCTAATTA TGCAAAGGGT GACCTGGACA TATCTTACAT CACATCCAGA
ATTGCAGTGA TGTCATTCCC AGCAGAAGGT GTGGAGTCAG CGCTCAAAAA CAACATCGAA
GATGTGCGGT TGTTCCTGGA CTCCAAGCAC CCAGGGCACT ATGCCGTCTA CAACCTGTCC
CCGAGGACCT ACCGGCCCTC CAGGTTCCAC AACCGGGTCT CCGAGTGTGG CTGGGCAGCA
CGGCGGGCCC CACACCTGCA CACCCTGTAC AACATCTGCA GGAACATGCA CGCCTGGCTG
CGGCAGGACC ACAAGAACGT CTGCGTCGTG CACTGCATGG ACGGGAGAGC CGCGTCTGCT
GTGGCCGTCT GCTCCTTCCT GTGCTTCTGC CGTCTCTTCA GCACCGCGGA GGCCGCCGTG
TACATGTTCA GCATGAAGCG CTGCCCACCA GGCATCTGGC CATCCCACAA AAGGTACATC
GAGTACATGT GTGACATGGT GGCGGAGGAG CCCATCACAC CCCACAGCAA GCCCATCCTG
GTGAGGGCCG TGGTCATGAC ACCCGTGCCG CTGTTCAGCA AGCAGAGGAG CGGCTGCAGG
CCCTTCTGCG AGGTCTACGT GGGGGACGAG CGTGTGGCCA GCACCTCCCA GGAGTACGAC
AAGATGCGGG ACTTTAAGAT TGAAGATGGC AAAGCGGTGA TTCCCCTGGG CGTCACGGTG
CAAGGAGACG TGCTCATCGT CATCTATCAC GCCCGGTCCA CTCTGGGCGG CCGGCTGCAG
GCCAAGATGG CATCCATGAA GATGTTCCAG ATTCAGTTCC ACACGGGGTT TGTGCCTCGG
AACGCCACCA CTGTGAAATT TGCCAAGTAT GACCTGGACG CGTGTGACAT TCAAGAAAAA
TACCCGGATT TATTTCAAGT GAACCTGGAA GTGGAGGTGG AGCCCAGGGA CAGGCCGAGC
CGGGAAGCCC CACCATGGGA GAACTCGAGC ATGAGGGGGC TGAACCCCAA AATCCTGTTT
TCCAGCCGGG AGGAGCAGCA AGACATTCTG TCTAAGTTTG GGAAGCCGGA GCTTCCCCGG
CAGCCTGGCT CCACGGCTCA GTATGATGCT GGGGCAGGGT CCCCGGAAGC CGAACCCACA
GACTCTGACT CACCGCCAAG CAGCAGCGCG GACGCCAGTC GCTTCCTGCA CACGCTGGAC
TGGCAGGAAG AGAAGGAGGC AGAGACTGGT GCAGAAAATG CCTCTTCCAA GGAGAGCGAG
TCTGCCCTGA TGGAGGACAG AGACGAGAGT GAGGTGTCAG ATGAAGGGGG ATCCCCGATC
TCCAGCGAGG GCCAGGAACC CAGGGCCGAC CCAGAGCCCC CCGGCCTGGC AGCAGGGCTG
GTGCAGCAGG ACTTGGTTTT TGAGGTGGAG ACACCGGCTG TGCTGCCAGA GCCTGTGCCA
CAGGAAGACG GGGTCGACCT CCTGGGCCTG CACTCCGAGG TGGGCGCAGG GCCAGCTGTA
CCCCCGCAGG CCTGCAAGGC CCCCTCCAGC AACACCGACC TGCTCAGCTG CCTCCTTGGG
CCCCCTGAGG CCGCCTCCCA GGGGCCCCCG GAGGATCTGC TCAGCGAGGA CCCGCTGCTC
CTGGCAAGCC CGGCCCCTCC CCTGAGCGTG CAGAGCACCC CAAGAGGAGG GCCCCCTGCC
GCTGCTGACC CCTTTGGCCC GCTTCTGCCG TCTTCAGGCA ACAACTCCCA GCCCTGCTCC
AATCCTGATC TCTTCGGCGA ATTTCTCAAT TCGGACTCTG TGACCGTCCC ACCATCCTTC
CCGTCTGCCC ACAGTGCTCC GCCCCCATCC TGCAGCGCCG ACTTCCTGCA CCTGGGGGAT
CTGCCAGGAG AGCCCAGCAA GATGACAGCC TCGTCCAGCA ACCCAGACCT GCTGGGAGGA
TGGGCTGCCT GGACCGAGAC TGCAGCATCG GCAGTGGCCC CCACGCCAGC CACAGAAGGC
CCCCTCTTCT CTCCTGGAGG TCAGCCGGCC CCTTGTGGCT CTCAGGCCAG CTGGACCAAG
TCTCAGAACC CGGACCCATT TGCTGACCTT GGCGACCTCA GCTCCGGCCT CCAAGACCCC
CAAGCCCAGA GCACAGTGAG CCCAAGGGGA CAGCGTGTCT GCACCTGTTC CAGGCGACTG
CCAACTGGCA AGCTAAAACC GGGAGTTGCT GACACTGGCA CTGCTGCCAG CCCCCACCGG
CATTGTGGCT CACCAGCTGG ATTCCCTCCT GGGGGCTTCA TTCCCAAAAC GGCCACCACG
CCCAAAGGCA GCAGCTCCTG GCAGACAAGT CGGCCGCCAG CCCAGGGCGC CTCATGGCCC
CCTCAGGCCA AGCCGCCCCC CAAAGCCTGC ACACAGCCAA GGCCTAACTA TGCCTCGAAC
TTCAGTGTGA TCGGGGCGCG GGAGGAGCGG GGGGTCCGCG CACCCAGCTT TGCTCAAAAG
CCAAAAGTCT CTGAGAACGA CTTTGAAGAT CTGTTGTCCA ATCAAGGCTT CTCCTCCAGG
TCTGACAAGA AAGGGCCAAA GACCATTGCA GAGATGAGGA AGCAGGACCT GGCTAAAGAC
ACGGACCCAC TCAAGCTGAA GCTCCTGGAC TGGATTGAGG GCAAGGAGCG GAACATCCGG
GCCCTGCTGT CCACGCTGCA CACAGTGCTG TGGGACGGGG AGAGCCGCTG GACGCCCGTG
GGCATGGCCG ACCTGGTGGC TCCGGAGCAA GTGAAGAAGC ACTATCGCCG CGCGGTGCTG
GCTGTGCACC CCGACAAGGC TGCGGGGCAG CCGTACGAGC AGCACGCCAA GATGATCTTC
ATGGAGCTGA ATGACGCCTG GTCGGAGTTT GAGAACCAGG GCTCCCGGCC CCTCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57903
Clone ID Related Accession (Same CDS sequence) XM_011513425.2
Accession Version XM_011513425.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4068bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product cyclin-G-associated kinase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011513425.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATGTG
GGGAGTGGCA GAGAGTATGC ATTAAAGAGG CTATTATCCA ATGAAGAGGA AAAGAACAGA
GCCATCATTC AAGAAGTTTG CTTCATGAAA AAGCTTTCCG GCCACCCGAA CATTGTCCAG
TTTTGTTCTG CAGCGTCTAT AGGAAAAGAG GAGTCAGACA CGGGGCAGGC TGAGTTCCTC
TTGCTCACAG AGCTCTGTAA AGGGCAGCTG GTGGAATTTT TGAAGAAAAT GGAATCTCGA
GGCCCCCTTT CGTGCGACAC GGTTCTGAAG ATCTTCTACC AGACGTGCCG CGCCGTGCAG
CACATGCACC GGCAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA GAACTTGTTG
CTTAGTAACC AAGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACGAC CATCTCGCAC
TACCCTGACT ACAGCTGGAG CGCCCAGAGG CGAGCCCTGG TGGAGGAAGA GATCACGAGG
AATACAACAC CAATGTATAG AACACCAGAA ATCATAGACT TGTATTCCAA CTTCCCGATC
GGCGAGAAGC AGGATATCTG GGCCCTGGGC TGCATCTTGT ACCTGCTGTG CTTCCGGCAG
CACCCTTTTG AGGATGGAGC GAAACTTCGA ATAGTCAATG GGAAGTACTC GATCCCCCCG
CACGACACGC AGTACACGGT CTTCCACAGC CTCATCCGCG CCATGCTGCA GGTGAACCCG
GAGGAGCGGC TGTCCATCGC CGAGGTGGTG CACCAGCTGC AGGAGATCGC GGCCGCCCGC
AACGTGAACC CCAAGTCTCC CATCACAGAG CTCCTGGAGC AGAATGGAGG CTACGGGAGC
GCCACACTGT CCCGAGGGCC ACCCCCTCCC GTGGGCCCCG CTGGCAGTGG CTACAGTGGA
GGCCTGGCGC TGGCGGAGTA CGACCAGCCG TATGGCGGCT TCCTGGACAT TCTGCGGGGT
GGGACAGAGC GGCTCTTCAC CAACCTCAAG GACACCTCCT CCAAGGTCAT CCAGTCCGTC
GCTAATTATG CAAAGGGTGA CCTGGACATA TCTTACATCA CATCCAGAAT TGCAGTGATG
TCATTCCCAG CAGAAGGTGT GGAGTCAGCG CTCAAAAACA ACATCGAAGA TGTGCGGTTG
TTCCTGGACT CCAAGCACCC AGGGCACTAT GCCGTCTACA ACCTGTCCCC GAGGACCTAC
CGGCCCTCCA GGTTCCACAA CCGGGTCTCC GAGTGTGGCT GGGCAGCACG GCGGGCCCCA
CACCTGCACA CCCTGTACAA CATCTGCAGG AACATGCACG CCTGGCTGCG GCAGGACCAC
AAGAACGTCT GCGTCGTGCA CTGCATGGAC GGGAGAGCCG CGTCTGCTGT GGCCGTCTGC
TCCTTCCTGT GCTTCTGCCG TCTCTTCAGC ACCGCGGAGG CCGCCGTGTA CATGTTCAGC
ATGAAGCGCT GCCCACCAGG CATCTGGCCA TCCCACAAAA GGTACATCGA GTACATGTGT
GACATGGTGG CGGAGGAGCC CATCACACCC CACAGCAAGC CCATCCTGGT GAGGGCCGTG
GTCATGACAC CCGTGCCGCT GTTCAGCAAG CAGAGGAGCG GCTGCAGGCC CTTCTGCGAG
GTCTACGTGG GGGACGAGCG TGTGGCCAGC ACCTCCCAGG AGTACGACAA GATGCGGGAC
TTTAAGATTG AAGATGGCAA AGCGGTGATT CCCCTGGGCG TCACGGTGCA AGGAGACGTG
CTCATCGTCA TCTATCACGC CCGGTCCACT CTGGGCGGCC GGCTGCAGGC CAAGATGGCA
TCCATGAAGA TGTTCCAGAT TCAGTTCCAC ACGGGGTTTG TGCCTCGGAA CGCCACCACT
GTGAAATTTG CCAAGTATGA CCTGGACGCG TGTGACATTC AAGAAAAATA CCCGGATTTA
TTTCAAGTGA ACCTGGAAGT GGAGGTGGAG CCCAGGGACA GGCCGAGCCG GGAAGCCCCA
CCATGGGAGA ACTCGAGCAT GAGGGGGCTG AACCCCAAAA TCCTGTTTTC CAGCCGGGAG
GAGCAGCAAG ACATTCTGTC TAAGTTTGGG AAGCCGGAGC TTCCCCGGCA GCCTGGCTCC
ACGGCTCAGT ATGATGCTGG GGCAGGGTCC CCGGAAGCCG AACCCACAGA CTCTGACTCA
CCGCCAAGCA GCAGCGCGGA CGCCAGTCGC TTCCTGCACA CGCTGGACTG GCAGGAAGAG
AAGGAGGCAG AGACTGGTGC AGAAAATGCC TCTTCCAAGG AGAGCGAGTC TGCCCTGATG
GAGGACAGAG ACGAGAGTGA GGTGTCAGAT GAAGGGGGAT CCCCGATCTC CAGCGAGGGC
CAGGAACCCA GGGCCGACCC AGAGCCCCCC GGCCTGGCAG CAGGGCTGGT GCAGCAGGAC
TTGGTTTTTG AGGTGGAGAC ACCGGCTGTG CTGCCAGAGC CTGTGCCACA GGAAGACGGG
GTCGACCTCC TGGGCCTGCA CTCCGAGGTG GGCGCAGGGC CAGCTGTACC CCCGCAGGCC
TGCAAGGCCC CCTCCAGCAA CACCGACCTG CTCAGCTGCC TCCTTGGGCC CCCTGAGGCC
GCCTCCCAGG GGCCCCCGGA GGATCTGCTC AGCGAGGACC CGCTGCTCCT GGCAAGCCCG
GCCCCTCCCC TGAGCGTGCA GAGCACCCCA AGAGGAGGGC CCCCTGCCGC TGCTGACCCC
TTTGGCCCGC TTCTGCCGTC TTCAGGCAAC AACTCCCAGC CCTGCTCCAA TCCTGATCTC
TTCGGCGAAT TTCTCAATTC GGACTCTGTG ACCGTCCCAC CATCCTTCCC GTCTGCCCAC
AGTGCTCCGC CCCCATCCTG CAGCGCCGAC TTCCTGCACC TGGGGGATCT GCCAGGAGAG
CCCAGCAAGA TGACAGCCTC GTCCAGCAAC CCAGACCTGC TGGGAGGATG GGCTGCCTGG
ACCGAGACTG CAGCATCGGC AGTGGCCCCC ACGCCAGCCA CAGAAGGCCC CCTCTTCTCT
CCTGGAGGTC AGCCGGCCCC TTGTGGCTCT CAGGCCAGCT GGACCAAGTC TCAGAACCCG
GACCCATTTG CTGACCTTGG CGACCTCAGC TCCGGCCTCC AAGACCCCCA AGCCCAGAGC
ACAGTGAGCC CAAGGGGACA GCGTGTCTGC ACCTGTTCCA GGCGACTGCC AACTGGCAAG
CTAAAACCGG GAGTTGCTGA CACTGGCACT GCTGCCAGCC CCCACCGGCA TTGTGGCTCA
CCAGCTGGAT TCCCTCCTGG GGGCTTCATT CCCAAAACGG CCACCACGCC CAAAGGCAGC
AGCTCCTGGC AGACAAGTCG GCCGCCAGCC CAGGGCGCCT CATGGCCCCC TCAGGCCAAG
CCGCCCCCCA AAGCCTGCAC ACAGCCAAGG CCTAACTATG CCTCGAACTT CAGTGTGATC
GGGGCGCGGG AGGAGCGGGG GGTCCGCGCA CCCAGCTTTG CTCAAAAGCC AAAAGTCTCT
GAGAACGACT TTGAAGATCT GTTGTCCAAT CAAGGCTTCT CCTCCAGGTC TGACAAGAAA
GGGCCAAAGA CCATTGCAGA GATGAGGAAG CAGGACCTGG CTAAAGACAC GGACCCACTC
AAGCTGAAGC TCCTGGACTG GATTGAGGGC AAGGAGCGGA ACATCCGGGC CCTGCTGTCC
ACGCTGCACA CAGTGCTGTG GGACGGGGAG AGCCGCTGGA CGCCCGTGGG CATGGCCGAC
CTGGTGGCTC CGGAGCAAGT GAAGAAGCAC TATCGCCGCG CGGTGCTGGC TGTGCACCCC
GACAAGGCTG CGGGGCAGCC GTACGAGCAG CACGCCAAGA TGATCTTCAT GGAGCTGAAT
GACGCCTGGT CGGAGTTTGA GAACCAGGGC TCCCGGCCCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011511727.1
CDS144..4211
Misc Feature(1)258..1103
Misc Feature(2)264..1070
Misc Feature(3)order(279..293,303..305,342..344,348..350,438..440,510..521,528..530,534..536,660..662,666..668,672..677,681..683,714..716,723..725,807..818)
Misc Feature(4)order(279..293,303..305,342..344,348..350,438..440,510..521,528..530,660..662,666..668,672..677,681..683,714..716)
Misc Feature(5)order(291..293,528..530,534..536,660..662,666..668,672..674,723..725,807..818)
Misc Feature(6)order(711..803,807..818)
Misc Feature(7)1851..2267
Misc Feature(8)<4053..4187
Misc Feature(9)order(4098..4106,4140..4142,4149..4154,4161..4166)
Translation

Target ORF information:

RefSeq Version XM_011513425.2
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin G associated kinase (GAK), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011513425.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATGTG
GGGAGTGGCA GAGAGTATGC ATTAAAGAGG CTATTATCCA ATGAAGAGGA AAAGAACAGA
GCCATCATTC AAGAAGTTTG CTTCATGAAA AAGCTTTCCG GCCACCCGAA CATTGTCCAG
TTTTGTTCTG CAGCGTCTAT AGGAAAAGAG GAGTCAGACA CGGGGCAGGC TGAGTTCCTC
TTGCTCACAG AGCTCTGTAA AGGGCAGCTG GTGGAATTTT TGAAGAAAAT GGAATCTCGA
GGCCCCCTTT CGTGCGACAC GGTTCTGAAG ATCTTCTACC AGACGTGCCG CGCCGTGCAG
CACATGCACC GGCAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA GAACTTGTTG
CTTAGTAACC AAGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACGAC CATCTCGCAC
TACCCTGACT ACAGCTGGAG CGCCCAGAGG CGAGCCCTGG TGGAGGAAGA GATCACGAGG
AATACAACAC CAATGTATAG AACACCAGAA ATCATAGACT TGTATTCCAA CTTCCCGATC
GGCGAGAAGC AGGATATCTG GGCCCTGGGC TGCATCTTGT ACCTGCTGTG CTTCCGGCAG
CACCCTTTTG AGGATGGAGC GAAACTTCGA ATAGTCAATG GGAAGTACTC GATCCCCCCG
CACGACACGC AGTACACGGT CTTCCACAGC CTCATCCGCG CCATGCTGCA GGTGAACCCG
GAGGAGCGGC TGTCCATCGC CGAGGTGGTG CACCAGCTGC AGGAGATCGC GGCCGCCCGC
AACGTGAACC CCAAGTCTCC CATCACAGAG CTCCTGGAGC AGAATGGAGG CTACGGGAGC
GCCACACTGT CCCGAGGGCC ACCCCCTCCC GTGGGCCCCG CTGGCAGTGG CTACAGTGGA
GGCCTGGCGC TGGCGGAGTA CGACCAGCCG TATGGCGGCT TCCTGGACAT TCTGCGGGGT
GGGACAGAGC GGCTCTTCAC CAACCTCAAG GACACCTCCT CCAAGGTCAT CCAGTCCGTC
GCTAATTATG CAAAGGGTGA CCTGGACATA TCTTACATCA CATCCAGAAT TGCAGTGATG
TCATTCCCAG CAGAAGGTGT GGAGTCAGCG CTCAAAAACA ACATCGAAGA TGTGCGGTTG
TTCCTGGACT CCAAGCACCC AGGGCACTAT GCCGTCTACA ACCTGTCCCC GAGGACCTAC
CGGCCCTCCA GGTTCCACAA CCGGGTCTCC GAGTGTGGCT GGGCAGCACG GCGGGCCCCA
CACCTGCACA CCCTGTACAA CATCTGCAGG AACATGCACG CCTGGCTGCG GCAGGACCAC
AAGAACGTCT GCGTCGTGCA CTGCATGGAC GGGAGAGCCG CGTCTGCTGT GGCCGTCTGC
TCCTTCCTGT GCTTCTGCCG TCTCTTCAGC ACCGCGGAGG CCGCCGTGTA CATGTTCAGC
ATGAAGCGCT GCCCACCAGG CATCTGGCCA TCCCACAAAA GGTACATCGA GTACATGTGT
GACATGGTGG CGGAGGAGCC CATCACACCC CACAGCAAGC CCATCCTGGT GAGGGCCGTG
GTCATGACAC CCGTGCCGCT GTTCAGCAAG CAGAGGAGCG GCTGCAGGCC CTTCTGCGAG
GTCTACGTGG GGGACGAGCG TGTGGCCAGC ACCTCCCAGG AGTACGACAA GATGCGGGAC
TTTAAGATTG AAGATGGCAA AGCGGTGATT CCCCTGGGCG TCACGGTGCA AGGAGACGTG
CTCATCGTCA TCTATCACGC CCGGTCCACT CTGGGCGGCC GGCTGCAGGC CAAGATGGCA
TCCATGAAGA TGTTCCAGAT TCAGTTCCAC ACGGGGTTTG TGCCTCGGAA CGCCACCACT
GTGAAATTTG CCAAGTATGA CCTGGACGCG TGTGACATTC AAGAAAAATA CCCGGATTTA
TTTCAAGTGA ACCTGGAAGT GGAGGTGGAG CCCAGGGACA GGCCGAGCCG GGAAGCCCCA
CCATGGGAGA ACTCGAGCAT GAGGGGGCTG AACCCCAAAA TCCTGTTTTC CAGCCGGGAG
GAGCAGCAAG ACATTCTGTC TAAGTTTGGG AAGCCGGAGC TTCCCCGGCA GCCTGGCTCC
ACGGCTCAGT ATGATGCTGG GGCAGGGTCC CCGGAAGCCG AACCCACAGA CTCTGACTCA
CCGCCAAGCA GCAGCGCGGA CGCCAGTCGC TTCCTGCACA CGCTGGACTG GCAGGAAGAG
AAGGAGGCAG AGACTGGTGC AGAAAATGCC TCTTCCAAGG AGAGCGAGTC TGCCCTGATG
GAGGACAGAG ACGAGAGTGA GGTGTCAGAT GAAGGGGGAT CCCCGATCTC CAGCGAGGGC
CAGGAACCCA GGGCCGACCC AGAGCCCCCC GGCCTGGCAG CAGGGCTGGT GCAGCAGGAC
TTGGTTTTTG AGGTGGAGAC ACCGGCTGTG CTGCCAGAGC CTGTGCCACA GGAAGACGGG
GTCGACCTCC TGGGCCTGCA CTCCGAGGTG GGCGCAGGGC CAGCTGTACC CCCGCAGGCC
TGCAAGGCCC CCTCCAGCAA CACCGACCTG CTCAGCTGCC TCCTTGGGCC CCCTGAGGCC
GCCTCCCAGG GGCCCCCGGA GGATCTGCTC AGCGAGGACC CGCTGCTCCT GGCAAGCCCG
GCCCCTCCCC TGAGCGTGCA GAGCACCCCA AGAGGAGGGC CCCCTGCCGC TGCTGACCCC
TTTGGCCCGC TTCTGCCGTC TTCAGGCAAC AACTCCCAGC CCTGCTCCAA TCCTGATCTC
TTCGGCGAAT TTCTCAATTC GGACTCTGTG ACCGTCCCAC CATCCTTCCC GTCTGCCCAC
AGTGCTCCGC CCCCATCCTG CAGCGCCGAC TTCCTGCACC TGGGGGATCT GCCAGGAGAG
CCCAGCAAGA TGACAGCCTC GTCCAGCAAC CCAGACCTGC TGGGAGGATG GGCTGCCTGG
ACCGAGACTG CAGCATCGGC AGTGGCCCCC ACGCCAGCCA CAGAAGGCCC CCTCTTCTCT
CCTGGAGGTC AGCCGGCCCC TTGTGGCTCT CAGGCCAGCT GGACCAAGTC TCAGAACCCG
GACCCATTTG CTGACCTTGG CGACCTCAGC TCCGGCCTCC AAGACCCCCA AGCCCAGAGC
ACAGTGAGCC CAAGGGGACA GCGTGTCTGC ACCTGTTCCA GGCGACTGCC AACTGGCAAG
CTAAAACCGG GAGTTGCTGA CACTGGCACT GCTGCCAGCC CCCACCGGCA TTGTGGCTCA
CCAGCTGGAT TCCCTCCTGG GGGCTTCATT CCCAAAACGG CCACCACGCC CAAAGGCAGC
AGCTCCTGGC AGACAAGTCG GCCGCCAGCC CAGGGCGCCT CATGGCCCCC TCAGGCCAAG
CCGCCCCCCA AAGCCTGCAC ACAGCCAAGG CCTAACTATG CCTCGAACTT CAGTGTGATC
GGGGCGCGGG AGGAGCGGGG GGTCCGCGCA CCCAGCTTTG CTCAAAAGCC AAAAGTCTCT
GAGAACGACT TTGAAGATCT GTTGTCCAAT CAAGGCTTCT CCTCCAGGTC TGACAAGAAA
GGGCCAAAGA CCATTGCAGA GATGAGGAAG CAGGACCTGG CTAAAGACAC GGACCCACTC
AAGCTGAAGC TCCTGGACTG GATTGAGGGC AAGGAGCGGA ACATCCGGGC CCTGCTGTCC
ACGCTGCACA CAGTGCTGTG GGACGGGGAG AGCCGCTGGA CGCCCGTGGG CATGGCCGAC
CTGGTGGCTC CGGAGCAAGT GAAGAAGCAC TATCGCCGCG CGGTGCTGGC TGTGCACCCC
GACAAGGCTG CGGGGCAGCC GTACGAGCAG CACGCCAAGA TGATCTTCAT GGAGCTGAAT
GACGCCTGGT CGGAGTTTGA GAACCAGGGC TCCCGGCCCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57904
Clone ID Related Accession (Same CDS sequence) XM_011513426.2
Accession Version XM_011513426.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4035bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product cyclin-G-associated kinase isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011513426.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATGTG
GGGAGTGGCA GAGAGTATGC ATTAAAGAGG CTATTATCCA ATGAAGAGGA AAAGAACAGA
GCCATCATTC AAGAAGTTTG CTTCATGAAA AAGCTTTCCG GCCACCCGAA CATTGTCCAG
TTTTGTTCTG CAGCGTCTAT AGGAAAAGAG GAGTCAGACA CGGGGCAGGC TGAGTTCCTC
TTGCTCACAG AGCTCTGTAA AGGGCAGCTG GTGGAATTTT TGAAGAAAAT GGAATCTCGA
GGCCCCCTTT CGTGCGACAC GGTTCTGAAG ATCTTCTACC AGACGTGCCG CGCCGTGCAG
CACATGCACC GGCAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA GAACTTGTTG
CTTAGTAACC AAGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACGAC CATCTCGCAC
TACCCTGACT ACAGCTGGAG CGCCCAGAGG CGAGCCCTGG TGGAGGAAGA GATCACGAGG
AATACAACAC CAATGTATAG AACACCAGAA ATCATAGACT TGTATTCCAA CTTCCCGATC
GGCGAGAAGC AGGATATCTG GGCCCTGGGC TGCATCTTGT ACCTGCTGTG CTTCCGGCAG
CACCCTTTTG AGGATGGAGC GAAACTTCGA ATAGTCAATG GGAAGTACTC GATCCCCCCG
CACGACACGC AGTACACGGT CTTCCACAGC CTCATCCGCG CCATGCTGCA GGTGAACCCG
GAGGAGCGGC TGTCCATCGC CGAGGTGGTG CACCAGCTGC AGGAGATCGC GGCCGCCCGC
AACGTGAACC CCAAGTCTCC CATCACAGAG CTCCTGGAGC AGAATGGAGG CTACGGGAGC
GCCACACTGT CCCGAGGGCC ACCCCCTCCC GTGGGCCCCG CTGGCAGTGG CTACAGTGGA
GGCCTGGCGC TGGCGGAGTA CGACCAGCCG TATGGCGGCT TCCTGGACAT TCTGCGGGGT
GGGACAGAGC GGCTCTTCAC CAACCTCAAG GACACCTCCT CCAAGGTCAT CCAGTCCGTC
GCTAATTATG CAAAGGGTGA CCTGGACATA TCTTACATCA CATCCAGAAT TGCAGTGATG
TCATTCCCAG CAGAAGGTGT GGAGTCAGCG CTCAAAAACA ACATCGAAGA TGTGCGGTTG
TTCCTGGACT CCAAGCACCC AGGGCACTAT GCCGTCTACA ACCTGTCCCC GAGGACCTAC
CGGCCCTCCA GGTTCCACAA CCGGGTCTCC GAGTGTGGCT GGGCAGCACG GCGGGCCCCA
CACCTGCACA CCCTGTACAA CATCTGCAGG AACATGCACG CCTGGCTGCG GCAGGACCAC
AAGAACGTCT GCGTCGTGCA CTGCATGGAC GGGAGAGCCG CGTCTGCTGT GGCCGTCTGC
TCCTTCCTGT GCTTCTGCCG TCTCTTCAGC ACCGCGGAGG CCGCCGTGTA CATGTTCAGC
ATGAAGCGCT GCCCACCAGG CATCTGGCCA TCCCACAAAA GGTACATCGA GTACATGTGT
GACATGGTGG CGGAGGAGCC CATCACACCC CACAGCAAGC CCATCCTGGT GAGGGCCGTG
GTCATGACAC CCGTGCCGCT GTTCAGCAAG CAGAGGAGCG GCTGCAGGCC CTTCTGCGAG
GTCTACGTGG GGGACGAGCG TGTGGCCAGC ACCTCCCAGG AGTACGACAA GATGCGGGAC
TTTAAGATTG AAGATGGCAA AGCGGTGATT CCCCTGGGCG TCACGGTGCA AGGAGACGTG
CTCATCGTCA TCTATCACGC CCGGTCCACT CTGGGCGGCC GGCTGCAGGC CAAGATGGCA
TCCATGAAGA TGTTCCAGAT TCAGTTCCAC ACGGGGTTTG TGCCTCGGAA CGCCACCACT
GTGAAATTTG CCAAGTATGA CCTGGACGCG TGTGACATTC AAGAAAAATA CCCGGATTTA
TTTCAAGTGA ACCTGGAAGT GGAGGTGGAG CCCAGGGACA GGCCGAGCCG GGAAGCCCCA
CCATGGGAGA ACTCGAGCAT GAGGGGGCTG AACCCCAAAA TCCTGTTTTC CAGCCGGGAG
GAGCAGCAAG ACATTCTGTC TAAGTTTGGG AAGCCGGAGC TTCCCCGGCA GCCTGGCTCC
ACGGCTCAGT ATGATGCTGG GGCAGGGTCC CCGGAAGCCG AACCCACAGA CTCTGACTCA
CCGCCAAGCA GCAGCGCGGA CGCCAGTCGC TTCCTGCACA CGCTGGACTG GCAGGAAGAG
AAGGAGGCAG AGACTGGTGC AGAAAATGCC TCTTCCAAGG AGAGCGAGTC TGCCCTGATG
GAGGACAGAG ACGAGAGTGA GGTGTCAGAT GAAGGGGGAT CCCCGATCTC CAGCGAGGGC
CAGGAACCCA GGGCCGACCC AGAGCCCCCC GGCCTGGCAG CAGGGCTGGT GCAGCAGGAC
TTGGTTTTTG AGGTGGAGAC ACCGGCTGTG CTGCCAGAGC CTGTGCCACA GGAAGACGGG
GTCGACCTCC TGGGCCTGCA CTCCGAGGTG GGCGCAGGGC CAGCTGTACC CCCGCAGGCC
TGCAAGGCCC CCTCCAGCAA CACCGACCTG CTCAGCTGCC TCCTTGGGCC CCCTGAGGCC
GCCTCCCAGG GGCCCCCGGA GGATCTGCTC AGCGAGGACC CGCTGCTCCT GGCAAGCCCG
GCCCCTCCCC TGAGCGTGCA GAGCACCCCA AGAGGAGGGC CCCCTGCCGC TGGCAACAAC
TCCCAGCCCT GCTCCAATCC TGATCTCTTC GGCGAATTTC TCAATTCGGA CTCTGTGACC
GTCCCACCAT CCTTCCCGTC TGCCCACAGT GCTCCGCCCC CATCCTGCAG CGCCGACTTC
CTGCACCTGG GGGATCTGCC AGGAGAGCCC AGCAAGATGA CAGCCTCGTC CAGCAACCCA
GACCTGCTGG GAGGATGGGC TGCCTGGACC GAGACTGCAG CATCGGCAGT GGCCCCCACG
CCAGCCACAG AAGGCCCCCT CTTCTCTCCT GGAGGTCAGC CGGCCCCTTG TGGCTCTCAG
GCCAGCTGGA CCAAGTCTCA GAACCCGGAC CCATTTGCTG ACCTTGGCGA CCTCAGCTCC
GGCCTCCAAG ACCCCCAAGC CCAGAGCACA GTGAGCCCAA GGGGACAGCG TGTCTGCACC
TGTTCCAGGC GACTGCCAAC TGGCAAGCTA AAACCGGGAG TTGCTGACAC TGGCACTGCT
GCCAGCCCCC ACCGGCATTG TGGCTCACCA GCTGGATTCC CTCCTGGGGG CTTCATTCCC
AAAACGGCCA CCACGCCCAA AGGCAGCAGC TCCTGGCAGA CAAGTCGGCC GCCAGCCCAG
GGCGCCTCAT GGCCCCCTCA GGCCAAGCCG CCCCCCAAAG CCTGCACACA GCCAAGGCCT
AACTATGCCT CGAACTTCAG TGTGATCGGG GCGCGGGAGG AGCGGGGGGT CCGCGCACCC
AGCTTTGCTC AAAAGCCAAA AGTCTCTGAG AACGACTTTG AAGATCTGTT GTCCAATCAA
GGCTTCTCCT CCAGGTCTGA CAAGAAAGGG CCAAAGACCA TTGCAGAGAT GAGGAAGCAG
GACCTGGCTA AAGACACGGA CCCACTCAAG CTGAAGCTCC TGGACTGGAT TGAGGGCAAG
GAGCGGAACA TCCGGGCCCT GCTGTCCACG CTGCACACAG TGCTGTGGGA CGGGGAGAGC
CGCTGGACGC CCGTGGGCAT GGCCGACCTG GTGGCTCCGG AGCAAGTGAA GAAGCACTAT
CGCCGCGCGG TGCTGGCTGT GCACCCCGAC AAGGCTGCGG GGCAGCCGTA CGAGCAGCAC
GCCAAGATGA TCTTCATGGA GCTGAATGAC GCCTGGTCGG AGTTTGAGAA CCAGGGCTCC
CGGCCCCTCT TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011511728.1
CDS144..4178
Misc Feature(1)258..1103
Misc Feature(2)264..1070
Misc Feature(3)order(279..293,303..305,342..344,348..350,438..440,510..521,528..530,534..536,660..662,666..668,672..677,681..683,714..716,723..725,807..818)
Misc Feature(4)order(279..293,303..305,342..344,348..350,438..440,510..521,528..530,660..662,666..668,672..677,681..683,714..716)
Misc Feature(5)order(291..293,528..530,534..536,660..662,666..668,672..674,723..725,807..818)
Misc Feature(6)order(711..803,807..818)
Misc Feature(7)1851..2267
Misc Feature(8)<4020..4154
Misc Feature(9)order(4065..4073,4107..4109,4116..4121,4128..4133)
Translation

Target ORF information:

RefSeq Version XM_011513426.2
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin G associated kinase (GAK), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011513426.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 
TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG
CGGGTGCGGC GGGTCCTGGC CGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATGTG
GGGAGTGGCA GAGAGTATGC ATTAAAGAGG CTATTATCCA ATGAAGAGGA AAAGAACAGA
GCCATCATTC AAGAAGTTTG CTTCATGAAA AAGCTTTCCG GCCACCCGAA CATTGTCCAG
TTTTGTTCTG CAGCGTCTAT AGGAAAAGAG GAGTCAGACA CGGGGCAGGC TGAGTTCCTC
TTGCTCACAG AGCTCTGTAA AGGGCAGCTG GTGGAATTTT TGAAGAAAAT GGAATCTCGA
GGCCCCCTTT CGTGCGACAC GGTTCTGAAG ATCTTCTACC AGACGTGCCG CGCCGTGCAG
CACATGCACC GGCAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA GAACTTGTTG
CTTAGTAACC AAGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACGAC CATCTCGCAC
TACCCTGACT ACAGCTGGAG CGCCCAGAGG CGAGCCCTGG TGGAGGAAGA GATCACGAGG
AATACAACAC CAATGTATAG AACACCAGAA ATCATAGACT TGTATTCCAA CTTCCCGATC
GGCGAGAAGC AGGATATCTG GGCCCTGGGC TGCATCTTGT ACCTGCTGTG CTTCCGGCAG
CACCCTTTTG AGGATGGAGC GAAACTTCGA ATAGTCAATG GGAAGTACTC GATCCCCCCG
CACGACACGC AGTACACGGT CTTCCACAGC CTCATCCGCG CCATGCTGCA GGTGAACCCG
GAGGAGCGGC TGTCCATCGC CGAGGTGGTG CACCAGCTGC AGGAGATCGC GGCCGCCCGC
AACGTGAACC CCAAGTCTCC CATCACAGAG CTCCTGGAGC AGAATGGAGG CTACGGGAGC
GCCACACTGT CCCGAGGGCC ACCCCCTCCC GTGGGCCCCG CTGGCAGTGG CTACAGTGGA
GGCCTGGCGC TGGCGGAGTA CGACCAGCCG TATGGCGGCT TCCTGGACAT TCTGCGGGGT
GGGACAGAGC GGCTCTTCAC CAACCTCAAG GACACCTCCT CCAAGGTCAT CCAGTCCGTC
GCTAATTATG CAAAGGGTGA CCTGGACATA TCTTACATCA CATCCAGAAT TGCAGTGATG
TCATTCCCAG CAGAAGGTGT GGAGTCAGCG CTCAAAAACA ACATCGAAGA TGTGCGGTTG
TTCCTGGACT CCAAGCACCC AGGGCACTAT GCCGTCTACA ACCTGTCCCC GAGGACCTAC
CGGCCCTCCA GGTTCCACAA CCGGGTCTCC GAGTGTGGCT GGGCAGCACG GCGGGCCCCA
CACCTGCACA CCCTGTACAA CATCTGCAGG AACATGCACG CCTGGCTGCG GCAGGACCAC
AAGAACGTCT GCGTCGTGCA CTGCATGGAC GGGAGAGCCG CGTCTGCTGT GGCCGTCTGC
TCCTTCCTGT GCTTCTGCCG TCTCTTCAGC ACCGCGGAGG CCGCCGTGTA CATGTTCAGC
ATGAAGCGCT GCCCACCAGG CATCTGGCCA TCCCACAAAA GGTACATCGA GTACATGTGT
GACATGGTGG CGGAGGAGCC CATCACACCC CACAGCAAGC CCATCCTGGT GAGGGCCGTG
GTCATGACAC CCGTGCCGCT GTTCAGCAAG CAGAGGAGCG GCTGCAGGCC CTTCTGCGAG
GTCTACGTGG GGGACGAGCG TGTGGCCAGC ACCTCCCAGG AGTACGACAA GATGCGGGAC
TTTAAGATTG AAGATGGCAA AGCGGTGATT CCCCTGGGCG TCACGGTGCA AGGAGACGTG
CTCATCGTCA TCTATCACGC CCGGTCCACT CTGGGCGGCC GGCTGCAGGC CAAGATGGCA
TCCATGAAGA TGTTCCAGAT TCAGTTCCAC ACGGGGTTTG TGCCTCGGAA CGCCACCACT
GTGAAATTTG CCAAGTATGA CCTGGACGCG TGTGACATTC AAGAAAAATA CCCGGATTTA
TTTCAAGTGA ACCTGGAAGT GGAGGTGGAG CCCAGGGACA GGCCGAGCCG GGAAGCCCCA
CCATGGGAGA ACTCGAGCAT GAGGGGGCTG AACCCCAAAA TCCTGTTTTC CAGCCGGGAG
GAGCAGCAAG ACATTCTGTC TAAGTTTGGG AAGCCGGAGC TTCCCCGGCA GCCTGGCTCC
ACGGCTCAGT ATGATGCTGG GGCAGGGTCC CCGGAAGCCG AACCCACAGA CTCTGACTCA
CCGCCAAGCA GCAGCGCGGA CGCCAGTCGC TTCCTGCACA CGCTGGACTG GCAGGAAGAG
AAGGAGGCAG AGACTGGTGC AGAAAATGCC TCTTCCAAGG AGAGCGAGTC TGCCCTGATG
GAGGACAGAG ACGAGAGTGA GGTGTCAGAT GAAGGGGGAT CCCCGATCTC CAGCGAGGGC
CAGGAACCCA GGGCCGACCC AGAGCCCCCC GGCCTGGCAG CAGGGCTGGT GCAGCAGGAC
TTGGTTTTTG AGGTGGAGAC ACCGGCTGTG CTGCCAGAGC CTGTGCCACA GGAAGACGGG
GTCGACCTCC TGGGCCTGCA CTCCGAGGTG GGCGCAGGGC CAGCTGTACC CCCGCAGGCC
TGCAAGGCCC CCTCCAGCAA CACCGACCTG CTCAGCTGCC TCCTTGGGCC CCCTGAGGCC
GCCTCCCAGG GGCCCCCGGA GGATCTGCTC AGCGAGGACC CGCTGCTCCT GGCAAGCCCG
GCCCCTCCCC TGAGCGTGCA GAGCACCCCA AGAGGAGGGC CCCCTGCCGC TGGCAACAAC
TCCCAGCCCT GCTCCAATCC TGATCTCTTC GGCGAATTTC TCAATTCGGA CTCTGTGACC
GTCCCACCAT CCTTCCCGTC TGCCCACAGT GCTCCGCCCC CATCCTGCAG CGCCGACTTC
CTGCACCTGG GGGATCTGCC AGGAGAGCCC AGCAAGATGA CAGCCTCGTC CAGCAACCCA
GACCTGCTGG GAGGATGGGC TGCCTGGACC GAGACTGCAG CATCGGCAGT GGCCCCCACG
CCAGCCACAG AAGGCCCCCT CTTCTCTCCT GGAGGTCAGC CGGCCCCTTG TGGCTCTCAG
GCCAGCTGGA CCAAGTCTCA GAACCCGGAC CCATTTGCTG ACCTTGGCGA CCTCAGCTCC
GGCCTCCAAG ACCCCCAAGC CCAGAGCACA GTGAGCCCAA GGGGACAGCG TGTCTGCACC
TGTTCCAGGC GACTGCCAAC TGGCAAGCTA AAACCGGGAG TTGCTGACAC TGGCACTGCT
GCCAGCCCCC ACCGGCATTG TGGCTCACCA GCTGGATTCC CTCCTGGGGG CTTCATTCCC
AAAACGGCCA CCACGCCCAA AGGCAGCAGC TCCTGGCAGA CAAGTCGGCC GCCAGCCCAG
GGCGCCTCAT GGCCCCCTCA GGCCAAGCCG CCCCCCAAAG CCTGCACACA GCCAAGGCCT
AACTATGCCT CGAACTTCAG TGTGATCGGG GCGCGGGAGG AGCGGGGGGT CCGCGCACCC
AGCTTTGCTC AAAAGCCAAA AGTCTCTGAG AACGACTTTG AAGATCTGTT GTCCAATCAA
GGCTTCTCCT CCAGGTCTGA CAAGAAAGGG CCAAAGACCA TTGCAGAGAT GAGGAAGCAG
GACCTGGCTA AAGACACGGA CCCACTCAAG CTGAAGCTCC TGGACTGGAT TGAGGGCAAG
GAGCGGAACA TCCGGGCCCT GCTGTCCACG CTGCACACAG TGCTGTGGGA CGGGGAGAGC
CGCTGGACGC CCGTGGGCAT GGCCGACCTG GTGGCTCCGG AGCAAGTGAA GAAGCACTAT
CGCCGCGCGG TGCTGGCTGT GCACCCCGAC AAGGCTGCGG GGCAGCCGTA CGAGCAGCAC
GCCAAGATGA TCTTCATGGA GCTGAATGAC GCCTGGTCGG AGTTTGAGAA CCAGGGCTCC
CGGCCCCTCT TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Genome-wide haplotype association analysis identifies SERPINB9, SERPINE2, GAK, and HSP90B1 as novel risk genes for oral squamous cell carcinoma.
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Sun W,Lv W,Lv H,Zhang R,Jiang Y


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Quantitative assessment of the association between GAK rs1564282 C/T polymorphism and the risk of Parkinson's disease.
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Journal of virology89(8)4387-404(2015 Apr)
Neveu G,Ziv-Av A,Barouch-Bentov R,Berkerman E,Mulholland J,Einav S


 
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