HECTD1 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following HECTD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECTD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu84855 XM_017021149.2
Latest version!
Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OHu84856 XM_017021153.1
Latest version!
Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHu84856 XM_017021152.2
Latest version!
Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHu16051 NM_015382.4
Latest version!
Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $923.30
$1319.00
OHu16051 XM_005267502.3
Latest version!
Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $923.30
$1319.00
OHu47641 XM_017021148.2
Latest version!
Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu66519 XM_011536619.2
Latest version!
Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu66519 XM_024449526.1
Latest version!
Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu66520 XM_017021150.1
Latest version!
Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu66520 XM_024449527.1
Latest version!
Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu66521 XM_017021151.2
Latest version!
Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu66521 XM_011536621.2
Latest version!
Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu66522 XM_011536622.1
Latest version!
Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
View Old Accession Versions >>
OHu16051 XM_005267502.2 Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $923.30
$1319.00
OHu84856 XM_017021152.1 Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHu84855 XM_017021149.1 Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OHu47641 XM_017021148.1 Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu66521 XM_017021151.1 Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu16051 NM_015382.3 Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $923.30
$1319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu84855
    Clone ID Related Accession (Same CDS sequence) XM_017021149.2 , XM_017021149.1
    Accession Version XM_017021149.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017021149.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGC
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGGAAGTT AGGCCCTGAT
    AGTTCTGTTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT CAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAATTA ACTAATAAAG AAGCAGCTTC ACAACGACCT
    CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTGAGTTCTT TGTTGGCTGC TGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CGAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA
    GTGACTATGT CAACATCCAG TGTTACTAGC AGCAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTTG GTCAATCTTT AAGTAACACT TTAACCACCA GCCTCACATC AACTTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TATTGGCTGA GCTCGATGAT GATGAGGACT TACCTGAGCC AGATGAAGAA
    GATGATGAGA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGAT TCTGAGACGC
    CCATCCCTGC AACGTCGAGC TGGCTCCCGC TCTGATGTAA CGCATCATGC TGTTACCTCG
    CAGCTACCAC AGGTACCTGC TGGAGCAGGG AGCCGACCTA TTGGGGAGCA GGAAGAAGAA
    GAGTACGAAA CTAAAGGAGG ACGCCGGAGA ACATGGGATG ATGATTATGT GCTAAAGAGA
    CAGTTTTCTG CATTGGTTCC TGCTTTTGAT CCTAGACCTG GTCGTACTAA TGTCCAGCAG
    ACAACTGATC TAGAAATACC ACCCCCAGGG ACCCCTCATT CAGAGCTCTT GGAAGAAGTC
    GAATGTACTC CGTCACCTCG ATTAGCTCTC ACTTTGAAAG TAACAGGTCT TGGAACGACT
    CGTGAAGTTG AATTACCACT CACCAATTTC AGATCAACCA TCTTTTACTA TGTACAAAAA
    TTGCTTCAAT TGTCCTGTAA TGGCAATGTG AAATCAGATA AACTTAGGCG TATTTGGGAG
    CCCACATACA CAATCATGTA CAGAGAAATG AAGGATTCTG ATAAAGAAAA GGAAAATGGA
    AAAATGGGTT GCTGGTCTAT AGAGCATGTG GAGCAGTACC TTGGCACTGA TGAATTACCA
    AAGAATGACT TGATAACCTA CCTGCAGAAG AATGCAGACG CTGCTTTCCT GCGCCACTGG
    AAATTAACTG GCACTAATAA AAGTATTAGG AAAAACAGAA ATTGTTCTCA GCTCATAGCT
    GCATATAAGG ATTTTTGTGA GCATGGAACA AAGTCTGGGT TAAACCAGGG GGCCATTTCT
    ACTCTTCAAA GTAGTGATAT TCTTAATTTA ACAAAAGAAC AACCTCAGGC CAAAGCAGGC
    AATGGACAGA ACTCTTGTGG AGTAGAAGAT GTCCTTCAGC TTCTGCGTAT TCTATATATA
    GTTGCAAGTG ACCCTTATTC AAGAATATCC CAGGAAGATG GTGATGAACA GCCTCAGTTT
    ACTTTTCCAC CAGATGAATT CACTAGCAAA AAAATTACAA CAAAAATATT ACAGCAGATT
    GAGGAACCAT TGGCACTGGC AAGTGGGGCT CTGCCAGACT GGTGTGAACA ATTAACCAGC
    AAATGTCCTT TTCTAATACC ATTTGAAACT AGACAGCTTT ATTTCACATG TACAGCATTT
    GGCGCCTCAA GAGCAATAGT ATGGTTACAG AACCGACGTG AAGCCACTGT GGAGCGAACG
    AGAACCACAA GCAGTGTTAG GCGAGATGAC CCTGGAGAGT TTCGAGTTGG TCGTCTCAAG
    CATGAAAGAG TAAAAGTTCC ACGTGGCGAG TCACTGATGG AATGGGCTGA GAATGTCATG
    CAAATACATG CAGATCGGAA ATCAGTTCTT GAGGTTGAAT TTTTAGGAGA AGAAGGAACT
    GGCTTGGGAC CCACATTAGA GTTTTATGCT CTGGTGGCAG CAGAATTCCA GAGAACTGAC
    TTGGGAGCTT GGCTTTGTGA TGATAATTTT CCAGATGATG AATCTCGTCA CGTTGATCTT
    GGAGGTGGAT TGAAACCTCC TGGATATTAT GTGCAGAGGT CATGTGGACT GTTCACAGCA
    CCATTTCCAC AGGATAGTGA TGAGCTTGAA AGGATCACGA AACTGTTTCA TTTCCTTGGA
    ATTTTCTTGG CCAAATGCAT TCAAGACAAT AGACTTGTGG ACTTACCTAT TTCTAAACCT
    TTTTTTAAAC TTATGTGTAT GGGTGACATT AAAAGCAATA TGAGTAAACT GATTTATGAG
    TCACGAGGTG ATAGAGACTT ACACTGTACT GAAAGTCAGT CTGAAGCTTC TACAGAAGAA
    GGTCATGATT CACTCTCGGT AGGAAGCTTT GAAGAGGATT CAAAATCAGA ATTTATTCTT
    GATCCCCCTA AACCAAAACC CCCAGCTTGG TTTAATGGAA TTTTGACTTG GGAAGACTTT
    GAATTAGTAA ACCCACACAG AGCCAGATTT TTAAAAGAAA TTAAAGACCT TGCTATCAAG
    AGGCGCCAAA TTTTAAGCAA CAAAGGTCTT TCTGAAGATG AGAAGAACAC AAAATTACAG
    GAACTAGTGC TGAAGAATCC ATCAGGTTCT GGGCCTCCAC TTAGCATAGA GGATTTAGGT
    TTAAATTTCC AGTTTTGCCC TTCCTCAAGA ATATATGGTT TTACAGCTGT GGATCTCAAG
    CCAAGTGGTG AAGATGAGAT GATAACAATG GATAATGCAG AAGAATATGT GGATTTGATG
    TTTGACTTTT GTATGCATAC GGGTATTCAG AAACAAATGG AAGCCTTTAG AGATGGGTTT
    AATAAAGTTT TTCCAATGGA GAAATTAAGT TCCTTCAGCC ATGAAGAAGT CCAAATGATT
    CTTTGTGGAA ACCAGTCACC ATCCTGGGCA GCAGAGGATA TTATCAATTA CACTGAACCT
    AAGCTGGGTT ATACACGTGA CAGCCCTGGT TTCCTGAGGT TTGTGAGGGT TTTATGTGGC
    ATGTCTTCTG ATGAAAGGAA AGCATTCTTG CAGTTTACCA CTGGTTGTTC AACTCTACCC
    CCAGGTGGAC TGGCTAACCT GCATCCCAGG CTCACGGTTG TACGCAAGGT TGATGCTACT
    GATGCAAGCT ATCCATCAGT CAATACATGT GTGCATTACC TTAAGTTGCC TGAATATTCT
    TCCGAGGAGA TCATGAGAGA GCGCCTGCTA GCTGCTACAA TGGAGAAAGG CTTTCATCTC
    AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876638.1
    CDS490..8175
    Translation

    Target ORF information:

    RefSeq Version XM_017021149.2
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017021149.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGC
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGGAAGTT AGGCCCTGAT
    AGTTCTGTTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT CAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAATTA ACTAATAAAG AAGCAGCTTC ACAACGACCT
    CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTGAGTTCTT TGTTGGCTGC TGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CGAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA
    GTGACTATGT CAACATCCAG TGTTACTAGC AGCAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTTG GTCAATCTTT AAGTAACACT TTAACCACCA GCCTCACATC AACTTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TATTGGCTGA GCTCGATGAT GATGAGGACT TACCTGAGCC AGATGAAGAA
    GATGATGAGA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGAT TCTGAGACGC
    CCATCCCTGC AACGTCGAGC TGGCTCCCGC TCTGATGTAA CGCATCATGC TGTTACCTCG
    CAGCTACCAC AGGTACCTGC TGGAGCAGGG AGCCGACCTA TTGGGGAGCA GGAAGAAGAA
    GAGTACGAAA CTAAAGGAGG ACGCCGGAGA ACATGGGATG ATGATTATGT GCTAAAGAGA
    CAGTTTTCTG CATTGGTTCC TGCTTTTGAT CCTAGACCTG GTCGTACTAA TGTCCAGCAG
    ACAACTGATC TAGAAATACC ACCCCCAGGG ACCCCTCATT CAGAGCTCTT GGAAGAAGTC
    GAATGTACTC CGTCACCTCG ATTAGCTCTC ACTTTGAAAG TAACAGGTCT TGGAACGACT
    CGTGAAGTTG AATTACCACT CACCAATTTC AGATCAACCA TCTTTTACTA TGTACAAAAA
    TTGCTTCAAT TGTCCTGTAA TGGCAATGTG AAATCAGATA AACTTAGGCG TATTTGGGAG
    CCCACATACA CAATCATGTA CAGAGAAATG AAGGATTCTG ATAAAGAAAA GGAAAATGGA
    AAAATGGGTT GCTGGTCTAT AGAGCATGTG GAGCAGTACC TTGGCACTGA TGAATTACCA
    AAGAATGACT TGATAACCTA CCTGCAGAAG AATGCAGACG CTGCTTTCCT GCGCCACTGG
    AAATTAACTG GCACTAATAA AAGTATTAGG AAAAACAGAA ATTGTTCTCA GCTCATAGCT
    GCATATAAGG ATTTTTGTGA GCATGGAACA AAGTCTGGGT TAAACCAGGG GGCCATTTCT
    ACTCTTCAAA GTAGTGATAT TCTTAATTTA ACAAAAGAAC AACCTCAGGC CAAAGCAGGC
    AATGGACAGA ACTCTTGTGG AGTAGAAGAT GTCCTTCAGC TTCTGCGTAT TCTATATATA
    GTTGCAAGTG ACCCTTATTC AAGAATATCC CAGGAAGATG GTGATGAACA GCCTCAGTTT
    ACTTTTCCAC CAGATGAATT CACTAGCAAA AAAATTACAA CAAAAATATT ACAGCAGATT
    GAGGAACCAT TGGCACTGGC AAGTGGGGCT CTGCCAGACT GGTGTGAACA ATTAACCAGC
    AAATGTCCTT TTCTAATACC ATTTGAAACT AGACAGCTTT ATTTCACATG TACAGCATTT
    GGCGCCTCAA GAGCAATAGT ATGGTTACAG AACCGACGTG AAGCCACTGT GGAGCGAACG
    AGAACCACAA GCAGTGTTAG GCGAGATGAC CCTGGAGAGT TTCGAGTTGG TCGTCTCAAG
    CATGAAAGAG TAAAAGTTCC ACGTGGCGAG TCACTGATGG AATGGGCTGA GAATGTCATG
    CAAATACATG CAGATCGGAA ATCAGTTCTT GAGGTTGAAT TTTTAGGAGA AGAAGGAACT
    GGCTTGGGAC CCACATTAGA GTTTTATGCT CTGGTGGCAG CAGAATTCCA GAGAACTGAC
    TTGGGAGCTT GGCTTTGTGA TGATAATTTT CCAGATGATG AATCTCGTCA CGTTGATCTT
    GGAGGTGGAT TGAAACCTCC TGGATATTAT GTGCAGAGGT CATGTGGACT GTTCACAGCA
    CCATTTCCAC AGGATAGTGA TGAGCTTGAA AGGATCACGA AACTGTTTCA TTTCCTTGGA
    ATTTTCTTGG CCAAATGCAT TCAAGACAAT AGACTTGTGG ACTTACCTAT TTCTAAACCT
    TTTTTTAAAC TTATGTGTAT GGGTGACATT AAAAGCAATA TGAGTAAACT GATTTATGAG
    TCACGAGGTG ATAGAGACTT ACACTGTACT GAAAGTCAGT CTGAAGCTTC TACAGAAGAA
    GGTCATGATT CACTCTCGGT AGGAAGCTTT GAAGAGGATT CAAAATCAGA ATTTATTCTT
    GATCCCCCTA AACCAAAACC CCCAGCTTGG TTTAATGGAA TTTTGACTTG GGAAGACTTT
    GAATTAGTAA ACCCACACAG AGCCAGATTT TTAAAAGAAA TTAAAGACCT TGCTATCAAG
    AGGCGCCAAA TTTTAAGCAA CAAAGGTCTT TCTGAAGATG AGAAGAACAC AAAATTACAG
    GAACTAGTGC TGAAGAATCC ATCAGGTTCT GGGCCTCCAC TTAGCATAGA GGATTTAGGT
    TTAAATTTCC AGTTTTGCCC TTCCTCAAGA ATATATGGTT TTACAGCTGT GGATCTCAAG
    CCAAGTGGTG AAGATGAGAT GATAACAATG GATAATGCAG AAGAATATGT GGATTTGATG
    TTTGACTTTT GTATGCATAC GGGTATTCAG AAACAAATGG AAGCCTTTAG AGATGGGTTT
    AATAAAGTTT TTCCAATGGA GAAATTAAGT TCCTTCAGCC ATGAAGAAGT CCAAATGATT
    CTTTGTGGAA ACCAGTCACC ATCCTGGGCA GCAGAGGATA TTATCAATTA CACTGAACCT
    AAGCTGGGTT ATACACGTGA CAGCCCTGGT TTCCTGAGGT TTGTGAGGGT TTTATGTGGC
    ATGTCTTCTG ATGAAAGGAA AGCATTCTTG CAGTTTACCA CTGGTTGTTC AACTCTACCC
    CCAGGTGGAC TGGCTAACCT GCATCCCAGG CTCACGGTTG TACGCAAGGT TGATGCTACT
    GATGCAAGCT ATCCATCAGT CAATACATGT GTGCATTACC TTAAGTTGCC TGAATATTCT
    TCCGAGGAGA TCATGAGAGA GCGCCTGCTA GCTGCTACAA TGGAGAAAGG CTTTCATCTC
    AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu84855
    Clone ID Related Accession (Same CDS sequence) XM_017021149.2 , XM_017021149.1
    Accession Version XM_017021149.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGC
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGGAAGTT AGGCCCTGAT
    AGTTCTGTTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT CAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAATTA ACTAATAAAG AAGCAGCTTC ACAACGACCT
    CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTGAGTTCTT TGTTGGCTGC TGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CGAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA
    GTGACTATGT CAACATCCAG TGTTACTAGC AGCAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTTG GTCAATCTTT AAGTAACACT TTAACCACCA GCCTCACATC AACTTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TATTGGCTGA GCTCGATGAT GATGAGGACT TACCTGAGCC AGATGAAGAA
    GATGATGAGA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGAT TCTGAGACGC
    CCATCCCTGC AACGTCGAGC TGGCTCCCGC TCTGATGTAA CGCATCATGC TGTTACCTCG
    CAGCTACCAC AGGTACCTGC TGGAGCAGGG AGCCGACCTA TTGGGGAGCA GGAAGAAGAA
    GAGTACGAAA CTAAAGGAGG ACGCCGGAGA ACATGGGATG ATGATTATGT GCTAAAGAGA
    CAGTTTTCTG CATTGGTTCC TGCTTTTGAT CCTAGACCTG GTCGTACTAA TGTCCAGCAG
    ACAACTGATC TAGAAATACC ACCCCCAGGG ACCCCTCATT CAGAGCTCTT GGAAGAAGTC
    GAATGTACTC CGTCACCTCG ATTAGCTCTC ACTTTGAAAG TAACAGGTCT TGGAACGACT
    CGTGAAGTTG AATTACCACT CACCAATTTC AGATCAACCA TCTTTTACTA TGTACAAAAA
    TTGCTTCAAT TGTCCTGTAA TGGCAATGTG AAATCAGATA AACTTAGGCG TATTTGGGAG
    CCCACATACA CAATCATGTA CAGAGAAATG AAGGATTCTG ATAAAGAAAA GGAAAATGGA
    AAAATGGGTT GCTGGTCTAT AGAGCATGTG GAGCAGTACC TTGGCACTGA TGAATTACCA
    AAGAATGACT TGATAACCTA CCTGCAGAAG AATGCAGACG CTGCTTTCCT GCGCCACTGG
    AAATTAACTG GCACTAATAA AAGTATTAGG AAAAACAGAA ATTGTTCTCA GCTCATAGCT
    GCATATAAGG ATTTTTGTGA GCATGGAACA AAGTCTGGGT TAAACCAGGG GGCCATTTCT
    ACTCTTCAAA GTAGTGATAT TCTTAATTTA ACAAAAGAAC AACCTCAGGC CAAAGCAGGC
    AATGGACAGA ACTCTTGTGG AGTAGAAGAT GTCCTTCAGC TTCTGCGTAT TCTATATATA
    GTTGCAAGTG ACCCTTATTC AAGAATATCC CAGGAAGATG GTGATGAACA GCCTCAGTTT
    ACTTTTCCAC CAGATGAATT CACTAGCAAA AAAATTACAA CAAAAATATT ACAGCAGATT
    GAGGAACCAT TGGCACTGGC AAGTGGGGCT CTGCCAGACT GGTGTGAACA ATTAACCAGC
    AAATGTCCTT TTCTAATACC ATTTGAAACT AGACAGCTTT ATTTCACATG TACAGCATTT
    GGCGCCTCAA GAGCAATAGT ATGGTTACAG AACCGACGTG AAGCCACTGT GGAGCGAACG
    AGAACCACAA GCAGTGTTAG GCGAGATGAC CCTGGAGAGT TTCGAGTTGG TCGTCTCAAG
    CATGAAAGAG TAAAAGTTCC ACGTGGCGAG TCACTGATGG AATGGGCTGA GAATGTCATG
    CAAATACATG CAGATCGGAA ATCAGTTCTT GAGGTTGAAT TTTTAGGAGA AGAAGGAACT
    GGCTTGGGAC CCACATTAGA GTTTTATGCT CTGGTGGCAG CAGAATTCCA GAGAACTGAC
    TTGGGAGCTT GGCTTTGTGA TGATAATTTT CCAGATGATG AATCTCGTCA CGTTGATCTT
    GGAGGTGGAT TGAAACCTCC TGGATATTAT GTGCAGAGGT CATGTGGACT GTTCACAGCA
    CCATTTCCAC AGGATAGTGA TGAGCTTGAA AGGATCACGA AACTGTTTCA TTTCCTTGGA
    ATTTTCTTGG CCAAATGCAT TCAAGACAAT AGACTTGTGG ACTTACCTAT TTCTAAACCT
    TTTTTTAAAC TTATGTGTAT GGGTGACATT AAAAGCAATA TGAGTAAACT GATTTATGAG
    TCACGAGGTG ATAGAGACTT ACACTGTACT GAAAGTCAGT CTGAAGCTTC TACAGAAGAA
    GGTCATGATT CACTCTCGGT AGGAAGCTTT GAAGAGGATT CAAAATCAGA ATTTATTCTT
    GATCCCCCTA AACCAAAACC CCCAGCTTGG TTTAATGGAA TTTTGACTTG GGAAGACTTT
    GAATTAGTAA ACCCACACAG AGCCAGATTT TTAAAAGAAA TTAAAGACCT TGCTATCAAG
    AGGCGCCAAA TTTTAAGCAA CAAAGGTCTT TCTGAAGATG AGAAGAACAC AAAATTACAG
    GAACTAGTGC TGAAGAATCC ATCAGGTTCT GGGCCTCCAC TTAGCATAGA GGATTTAGGT
    TTAAATTTCC AGTTTTGCCC TTCCTCAAGA ATATATGGTT TTACAGCTGT GGATCTCAAG
    CCAAGTGGTG AAGATGAGAT GATAACAATG GATAATGCAG AAGAATATGT GGATTTGATG
    TTTGACTTTT GTATGCATAC GGGTATTCAG AAACAAATGG AAGCCTTTAG AGATGGGTTT
    AATAAAGTTT TTCCAATGGA GAAATTAAGT TCCTTCAGCC ATGAAGAAGT CCAAATGATT
    CTTTGTGGAA ACCAGTCACC ATCCTGGGCA GCAGAGGATA TTATCAATTA CACTGAACCT
    AAGCTGGGTT ATACACGTGA CAGCCCTGGT TTCCTGAGGT TTGTGAGGGT TTTATGTGGC
    ATGTCTTCTG ATGAAAGGAA AGCATTCTTG CAGTTTACCA CTGGTTGTTC AACTCTACCC
    CCAGGTGGAC TGGCTAACCT GCATCCCAGG CTCACGGTTG TACGCAAGGT TGATGCTACT
    GATGCAAGCT ATCCATCAGT CAATACATGT GTGCATTACC TTAAGTTGCC TGAATATTCT
    TCCGAGGAGA TCATGAGAGA GCGCCTGCTA GCTGCTACAA TGGAGAAAGG CTTTCATCTC
    AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876638.1
    CDS22..7707
    Translation

    Target ORF information:

    RefSeq Version XM_017021149.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017021149.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGC
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGGAAGTT AGGCCCTGAT
    AGTTCTGTTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT CAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAATTA ACTAATAAAG AAGCAGCTTC ACAACGACCT
    CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTGAGTTCTT TGTTGGCTGC TGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CGAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA
    GTGACTATGT CAACATCCAG TGTTACTAGC AGCAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTTG GTCAATCTTT AAGTAACACT TTAACCACCA GCCTCACATC AACTTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TATTGGCTGA GCTCGATGAT GATGAGGACT TACCTGAGCC AGATGAAGAA
    GATGATGAGA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGAT TCTGAGACGC
    CCATCCCTGC AACGTCGAGC TGGCTCCCGC TCTGATGTAA CGCATCATGC TGTTACCTCG
    CAGCTACCAC AGGTACCTGC TGGAGCAGGG AGCCGACCTA TTGGGGAGCA GGAAGAAGAA
    GAGTACGAAA CTAAAGGAGG ACGCCGGAGA ACATGGGATG ATGATTATGT GCTAAAGAGA
    CAGTTTTCTG CATTGGTTCC TGCTTTTGAT CCTAGACCTG GTCGTACTAA TGTCCAGCAG
    ACAACTGATC TAGAAATACC ACCCCCAGGG ACCCCTCATT CAGAGCTCTT GGAAGAAGTC
    GAATGTACTC CGTCACCTCG ATTAGCTCTC ACTTTGAAAG TAACAGGTCT TGGAACGACT
    CGTGAAGTTG AATTACCACT CACCAATTTC AGATCAACCA TCTTTTACTA TGTACAAAAA
    TTGCTTCAAT TGTCCTGTAA TGGCAATGTG AAATCAGATA AACTTAGGCG TATTTGGGAG
    CCCACATACA CAATCATGTA CAGAGAAATG AAGGATTCTG ATAAAGAAAA GGAAAATGGA
    AAAATGGGTT GCTGGTCTAT AGAGCATGTG GAGCAGTACC TTGGCACTGA TGAATTACCA
    AAGAATGACT TGATAACCTA CCTGCAGAAG AATGCAGACG CTGCTTTCCT GCGCCACTGG
    AAATTAACTG GCACTAATAA AAGTATTAGG AAAAACAGAA ATTGTTCTCA GCTCATAGCT
    GCATATAAGG ATTTTTGTGA GCATGGAACA AAGTCTGGGT TAAACCAGGG GGCCATTTCT
    ACTCTTCAAA GTAGTGATAT TCTTAATTTA ACAAAAGAAC AACCTCAGGC CAAAGCAGGC
    AATGGACAGA ACTCTTGTGG AGTAGAAGAT GTCCTTCAGC TTCTGCGTAT TCTATATATA
    GTTGCAAGTG ACCCTTATTC AAGAATATCC CAGGAAGATG GTGATGAACA GCCTCAGTTT
    ACTTTTCCAC CAGATGAATT CACTAGCAAA AAAATTACAA CAAAAATATT ACAGCAGATT
    GAGGAACCAT TGGCACTGGC AAGTGGGGCT CTGCCAGACT GGTGTGAACA ATTAACCAGC
    AAATGTCCTT TTCTAATACC ATTTGAAACT AGACAGCTTT ATTTCACATG TACAGCATTT
    GGCGCCTCAA GAGCAATAGT ATGGTTACAG AACCGACGTG AAGCCACTGT GGAGCGAACG
    AGAACCACAA GCAGTGTTAG GCGAGATGAC CCTGGAGAGT TTCGAGTTGG TCGTCTCAAG
    CATGAAAGAG TAAAAGTTCC ACGTGGCGAG TCACTGATGG AATGGGCTGA GAATGTCATG
    CAAATACATG CAGATCGGAA ATCAGTTCTT GAGGTTGAAT TTTTAGGAGA AGAAGGAACT
    GGCTTGGGAC CCACATTAGA GTTTTATGCT CTGGTGGCAG CAGAATTCCA GAGAACTGAC
    TTGGGAGCTT GGCTTTGTGA TGATAATTTT CCAGATGATG AATCTCGTCA CGTTGATCTT
    GGAGGTGGAT TGAAACCTCC TGGATATTAT GTGCAGAGGT CATGTGGACT GTTCACAGCA
    CCATTTCCAC AGGATAGTGA TGAGCTTGAA AGGATCACGA AACTGTTTCA TTTCCTTGGA
    ATTTTCTTGG CCAAATGCAT TCAAGACAAT AGACTTGTGG ACTTACCTAT TTCTAAACCT
    TTTTTTAAAC TTATGTGTAT GGGTGACATT AAAAGCAATA TGAGTAAACT GATTTATGAG
    TCACGAGGTG ATAGAGACTT ACACTGTACT GAAAGTCAGT CTGAAGCTTC TACAGAAGAA
    GGTCATGATT CACTCTCGGT AGGAAGCTTT GAAGAGGATT CAAAATCAGA ATTTATTCTT
    GATCCCCCTA AACCAAAACC CCCAGCTTGG TTTAATGGAA TTTTGACTTG GGAAGACTTT
    GAATTAGTAA ACCCACACAG AGCCAGATTT TTAAAAGAAA TTAAAGACCT TGCTATCAAG
    AGGCGCCAAA TTTTAAGCAA CAAAGGTCTT TCTGAAGATG AGAAGAACAC AAAATTACAG
    GAACTAGTGC TGAAGAATCC ATCAGGTTCT GGGCCTCCAC TTAGCATAGA GGATTTAGGT
    TTAAATTTCC AGTTTTGCCC TTCCTCAAGA ATATATGGTT TTACAGCTGT GGATCTCAAG
    CCAAGTGGTG AAGATGAGAT GATAACAATG GATAATGCAG AAGAATATGT GGATTTGATG
    TTTGACTTTT GTATGCATAC GGGTATTCAG AAACAAATGG AAGCCTTTAG AGATGGGTTT
    AATAAAGTTT TTCCAATGGA GAAATTAAGT TCCTTCAGCC ATGAAGAAGT CCAAATGATT
    CTTTGTGGAA ACCAGTCACC ATCCTGGGCA GCAGAGGATA TTATCAATTA CACTGAACCT
    AAGCTGGGTT ATACACGTGA CAGCCCTGGT TTCCTGAGGT TTGTGAGGGT TTTATGTGGC
    ATGTCTTCTG ATGAAAGGAA AGCATTCTTG CAGTTTACCA CTGGTTGTTC AACTCTACCC
    CCAGGTGGAC TGGCTAACCT GCATCCCAGG CTCACGGTTG TACGCAAGGT TGATGCTACT
    GATGCAAGCT ATCCATCAGT CAATACATGT GTGCATTACC TTAAGTTGCC TGAATATTCT
    TCCGAGGAGA TCATGAGAGA GCGCCTGCTA GCTGCTACAA TGGAGAAAGG CTTTCATCTC
    AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu84856
    Clone ID Related Accession (Same CDS sequence) XM_017021153.1 , XM_017021152.1 , XM_017021152.2
    Accession Version XM_017021153.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6990bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCTG GTACTACATC AACAGTGACT ATGTCAACAT CCAGTGTTAC TAGCAGCAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTTGGTCAAT CTTTAAGTAA CACTTTAACC
    ACCAGCCTCA CATCAACTTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTATTGG CTGAGCTCGA TGATGATGAG
    GACTTACCTG AGCCAGATGA AGAAGATGAT GAGAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGATTCTGAG ACGCCCATCC CTGCAACGTC GAGCTGGCTC CCGCTCTGAT
    GTAACGCATC ATGCTGTTAC CTCGCAGCTA CCACAGGTAC CTGCTGGAGC AGGGAGCCGA
    CCTATTGGGG AGCAGGAAGA AGAAGAGTAC GAAACTAAAG GAGGACGCCG GAGAACATGG
    GATGATGATT ATGTGCTAAA GAGACAGTTT TCTGCATTGG TTCCTGCTTT TGATCCTAGA
    CCTGGTCGTA CTAATGTCCA GCAGACAACT GATCTAGAAA TACCACCCCC AGGGACCCCT
    CATTCAGAGC TCTTGGAAGA AGTCGAATGT ACTCCGTCAC CTCGATTAGC TCTCACTTTG
    AAAGTAACAG GTCTTGGAAC GACTCGTGAA GTTGAATTAC CACTCACCAA TTTCAGATCA
    ACCATCTTTT ACTATGTACA AAAATTGCTT CAATTGTCCT GTAATGGCAA TGTGAAATCA
    GATAAACTTA GGCGTATTTG GGAGCCCACA TACACAATCA TGTACAGAGA AATGAAGGAT
    TCTGATAAAG AAAAGGAAAA TGGAAAAATG GGTTGCTGGT CTATAGAGCA TGTGGAGCAG
    TACCTTGGCA CTGATGAATT ACCAAAGAAT GACTTGATAA CCTACCTGCA GAAGAATGCA
    GACGCTGCTT TCCTGCGCCA CTGGAAATTA ACTGGCACTA ATAAAAGTAT TAGGAAAAAC
    AGAAATTGTT CTCAGCTCAT AGCTGCATAT AAGGATTTTT GTGAGCATGG AACAAAGTCT
    GGGTTAAACC AGGGGGCCAT TTCTACTCTT CAAAGTAGTG ATATTCTTAA TTTAACAAAA
    GAACAACCTC AGGCCAAAGC AGGCAATGGA CAGAACTCTT GTGGAGTAGA AGATGTCCTT
    CAGCTTCTGC GTATTCTATA TATAGTTGCA AGTGACCCTT ATTCAAGAAT ATCCCAGGAA
    GATGGTGATG AACAGCCTCA GTTTACTTTT CCACCAGATG AATTCACTAG CAAAAAAATT
    ACAACAAAAA TATTACAGCA GATTGAGGAA CCATTGGCAC TGGCAAGTGG GGCTCTGCCA
    GACTGGTGTG AACAATTAAC CAGCAAATGT CCTTTTCTAA TACCATTTGA AACTAGACAG
    CTTTATTTCA CATGTACAGC ATTTGGCGCC TCAAGAGCAA TAGTATGGTT ACAGAACCGA
    CGTGAAGCCA CTGTGGAGCG AACGAGAACC ACAAGCAGTG TTAGGCGAGA TGACCCTGGA
    GAGTTTCGAG TTGGTCGTCT CAAGCATGAA AGAGTAAAAG TTCCACGTGG CGAGTCACTG
    ATGGAATGGG CTGAGAATGT CATGCAAATA CATGCAGATC GGAAATCAGT TCTTGAGGTT
    GAATTTTTAG GAGAAGAAGG AACTGGCTTG GGACCCACAT TAGAGTTTTA TGCTCTGGTG
    GCAGCAGAAT TCCAGAGAAC TGACTTGGGA GCTTGGCTTT GTGATGATAA TTTTCCAGAT
    GATGAATCTC GTCACGTTGA TCTTGGAGGT GGATTGAAAC CTCCTGGATA TTATGTGCAG
    AGGTCATGTG GACTGTTCAC AGCACCATTT CCACAGGATA GTGATGAGCT TGAAAGGATC
    ACGAAACTGT TTCATTTCCT TGGAATTTTC TTGGCCAAAT GCATTCAAGA CAATAGACTT
    GTGGACTTAC CTATTTCTAA ACCTTTTTTT AAACTTATGT GTATGGGTGA CATTAAAAGC
    AATATGAGTA AACTGATTTA TGAGTCACGA GGTGATAGAG ACTTACACTG TACTGAAAGT
    CAGTCTGAAG CTTCTACAGA AGAAGGTCAT GATTCACTCT CGGTAGGAAG CTTTGAAGAG
    GATTCAAAAT CAGAATTTAT TCTTGATCCC CCTAAACCAA AACCCCCAGC TTGGTTTAAT
    GGAATTTTGA CTTGGGAAGA CTTTGAATTA GTAAACCCAC ACAGAGCCAG ATTTTTAAAA
    GAAATTAAAG ACCTTGCTAT CAAGAGGCGC CAAATTTTAA GCAACAAAGG TCTTTCTGAA
    GATGAGAAGA ACACAAAATT ACAGGAACTA GTGCTGAAGA ATCCATCAGG TTCTGGGCCT
    CCACTTAGCA TAGAGGATTT AGGTTTAAAT TTCCAGTTTT GCCCTTCCTC AAGAATATAT
    GGTTTTACAG CTGTGGATCT CAAGCCAAGT GGTGAAGATG AGATGATAAC AATGGATAAT
    GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTTTGTATGC ATACGGGTAT TCAGAAACAA
    ATGGAAGCCT TTAGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC
    AGCCATGAAG AAGTCCAAAT GATTCTTTGT GGAAACCAGT CACCATCCTG GGCAGCAGAG
    GATATTATCA ATTACACTGA ACCTAAGCTG GGTTATACAC GTGACAGCCC TGGTTTCCTG
    AGGTTTGTGA GGGTTTTATG TGGCATGTCT TCTGATGAAA GGAAAGCATT CTTGCAGTTT
    ACCACTGGTT GTTCAACTCT ACCCCCAGGT GGACTGGCTA ACCTGCATCC CAGGCTCACG
    GTTGTACGCA AGGTTGATGC TACTGATGCA AGCTATCCAT CAGTCAATAC ATGTGTGCAT
    TACCTTAAGT TGCCTGAATA TTCTTCCGAG GAGATCATGA GAGAGCGCCT GCTAGCTGCT
    ACAATGGAGA AAGGCTTTCA TCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876642.1
    CDS128..7117
    Translation

    Target ORF information:

    RefSeq Version XM_017021153.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017021153.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCTG GTACTACATC AACAGTGACT ATGTCAACAT CCAGTGTTAC TAGCAGCAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTTGGTCAAT CTTTAAGTAA CACTTTAACC
    ACCAGCCTCA CATCAACTTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTATTGG CTGAGCTCGA TGATGATGAG
    GACTTACCTG AGCCAGATGA AGAAGATGAT GAGAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGATTCTGAG ACGCCCATCC CTGCAACGTC GAGCTGGCTC CCGCTCTGAT
    GTAACGCATC ATGCTGTTAC CTCGCAGCTA CCACAGGTAC CTGCTGGAGC AGGGAGCCGA
    CCTATTGGGG AGCAGGAAGA AGAAGAGTAC GAAACTAAAG GAGGACGCCG GAGAACATGG
    GATGATGATT ATGTGCTAAA GAGACAGTTT TCTGCATTGG TTCCTGCTTT TGATCCTAGA
    CCTGGTCGTA CTAATGTCCA GCAGACAACT GATCTAGAAA TACCACCCCC AGGGACCCCT
    CATTCAGAGC TCTTGGAAGA AGTCGAATGT ACTCCGTCAC CTCGATTAGC TCTCACTTTG
    AAAGTAACAG GTCTTGGAAC GACTCGTGAA GTTGAATTAC CACTCACCAA TTTCAGATCA
    ACCATCTTTT ACTATGTACA AAAATTGCTT CAATTGTCCT GTAATGGCAA TGTGAAATCA
    GATAAACTTA GGCGTATTTG GGAGCCCACA TACACAATCA TGTACAGAGA AATGAAGGAT
    TCTGATAAAG AAAAGGAAAA TGGAAAAATG GGTTGCTGGT CTATAGAGCA TGTGGAGCAG
    TACCTTGGCA CTGATGAATT ACCAAAGAAT GACTTGATAA CCTACCTGCA GAAGAATGCA
    GACGCTGCTT TCCTGCGCCA CTGGAAATTA ACTGGCACTA ATAAAAGTAT TAGGAAAAAC
    AGAAATTGTT CTCAGCTCAT AGCTGCATAT AAGGATTTTT GTGAGCATGG AACAAAGTCT
    GGGTTAAACC AGGGGGCCAT TTCTACTCTT CAAAGTAGTG ATATTCTTAA TTTAACAAAA
    GAACAACCTC AGGCCAAAGC AGGCAATGGA CAGAACTCTT GTGGAGTAGA AGATGTCCTT
    CAGCTTCTGC GTATTCTATA TATAGTTGCA AGTGACCCTT ATTCAAGAAT ATCCCAGGAA
    GATGGTGATG AACAGCCTCA GTTTACTTTT CCACCAGATG AATTCACTAG CAAAAAAATT
    ACAACAAAAA TATTACAGCA GATTGAGGAA CCATTGGCAC TGGCAAGTGG GGCTCTGCCA
    GACTGGTGTG AACAATTAAC CAGCAAATGT CCTTTTCTAA TACCATTTGA AACTAGACAG
    CTTTATTTCA CATGTACAGC ATTTGGCGCC TCAAGAGCAA TAGTATGGTT ACAGAACCGA
    CGTGAAGCCA CTGTGGAGCG AACGAGAACC ACAAGCAGTG TTAGGCGAGA TGACCCTGGA
    GAGTTTCGAG TTGGTCGTCT CAAGCATGAA AGAGTAAAAG TTCCACGTGG CGAGTCACTG
    ATGGAATGGG CTGAGAATGT CATGCAAATA CATGCAGATC GGAAATCAGT TCTTGAGGTT
    GAATTTTTAG GAGAAGAAGG AACTGGCTTG GGACCCACAT TAGAGTTTTA TGCTCTGGTG
    GCAGCAGAAT TCCAGAGAAC TGACTTGGGA GCTTGGCTTT GTGATGATAA TTTTCCAGAT
    GATGAATCTC GTCACGTTGA TCTTGGAGGT GGATTGAAAC CTCCTGGATA TTATGTGCAG
    AGGTCATGTG GACTGTTCAC AGCACCATTT CCACAGGATA GTGATGAGCT TGAAAGGATC
    ACGAAACTGT TTCATTTCCT TGGAATTTTC TTGGCCAAAT GCATTCAAGA CAATAGACTT
    GTGGACTTAC CTATTTCTAA ACCTTTTTTT AAACTTATGT GTATGGGTGA CATTAAAAGC
    AATATGAGTA AACTGATTTA TGAGTCACGA GGTGATAGAG ACTTACACTG TACTGAAAGT
    CAGTCTGAAG CTTCTACAGA AGAAGGTCAT GATTCACTCT CGGTAGGAAG CTTTGAAGAG
    GATTCAAAAT CAGAATTTAT TCTTGATCCC CCTAAACCAA AACCCCCAGC TTGGTTTAAT
    GGAATTTTGA CTTGGGAAGA CTTTGAATTA GTAAACCCAC ACAGAGCCAG ATTTTTAAAA
    GAAATTAAAG ACCTTGCTAT CAAGAGGCGC CAAATTTTAA GCAACAAAGG TCTTTCTGAA
    GATGAGAAGA ACACAAAATT ACAGGAACTA GTGCTGAAGA ATCCATCAGG TTCTGGGCCT
    CCACTTAGCA TAGAGGATTT AGGTTTAAAT TTCCAGTTTT GCCCTTCCTC AAGAATATAT
    GGTTTTACAG CTGTGGATCT CAAGCCAAGT GGTGAAGATG AGATGATAAC AATGGATAAT
    GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTTTGTATGC ATACGGGTAT TCAGAAACAA
    ATGGAAGCCT TTAGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC
    AGCCATGAAG AAGTCCAAAT GATTCTTTGT GGAAACCAGT CACCATCCTG GGCAGCAGAG
    GATATTATCA ATTACACTGA ACCTAAGCTG GGTTATACAC GTGACAGCCC TGGTTTCCTG
    AGGTTTGTGA GGGTTTTATG TGGCATGTCT TCTGATGAAA GGAAAGCATT CTTGCAGTTT
    ACCACTGGTT GTTCAACTCT ACCCCCAGGT GGACTGGCTA ACCTGCATCC CAGGCTCACG
    GTTGTACGCA AGGTTGATGC TACTGATGCA AGCTATCCAT CAGTCAATAC ATGTGTGCAT
    TACCTTAAGT TGCCTGAATA TTCTTCCGAG GAGATCATGA GAGAGCGCCT GCTAGCTGCT
    ACAATGGAGA AAGGCTTTCA TCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu84856
    Clone ID Related Accession (Same CDS sequence) XM_017021153.1 , XM_017021152.1 , XM_017021152.2
    Accession Version XM_017021152.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6990bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCTG GTACTACATC AACAGTGACT ATGTCAACAT CCAGTGTTAC TAGCAGCAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTTGGTCAAT CTTTAAGTAA CACTTTAACC
    ACCAGCCTCA CATCAACTTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTATTGG CTGAGCTCGA TGATGATGAG
    GACTTACCTG AGCCAGATGA AGAAGATGAT GAGAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGATTCTGAG ACGCCCATCC CTGCAACGTC GAGCTGGCTC CCGCTCTGAT
    GTAACGCATC ATGCTGTTAC CTCGCAGCTA CCACAGGTAC CTGCTGGAGC AGGGAGCCGA
    CCTATTGGGG AGCAGGAAGA AGAAGAGTAC GAAACTAAAG GAGGACGCCG GAGAACATGG
    GATGATGATT ATGTGCTAAA GAGACAGTTT TCTGCATTGG TTCCTGCTTT TGATCCTAGA
    CCTGGTCGTA CTAATGTCCA GCAGACAACT GATCTAGAAA TACCACCCCC AGGGACCCCT
    CATTCAGAGC TCTTGGAAGA AGTCGAATGT ACTCCGTCAC CTCGATTAGC TCTCACTTTG
    AAAGTAACAG GTCTTGGAAC GACTCGTGAA GTTGAATTAC CACTCACCAA TTTCAGATCA
    ACCATCTTTT ACTATGTACA AAAATTGCTT CAATTGTCCT GTAATGGCAA TGTGAAATCA
    GATAAACTTA GGCGTATTTG GGAGCCCACA TACACAATCA TGTACAGAGA AATGAAGGAT
    TCTGATAAAG AAAAGGAAAA TGGAAAAATG GGTTGCTGGT CTATAGAGCA TGTGGAGCAG
    TACCTTGGCA CTGATGAATT ACCAAAGAAT GACTTGATAA CCTACCTGCA GAAGAATGCA
    GACGCTGCTT TCCTGCGCCA CTGGAAATTA ACTGGCACTA ATAAAAGTAT TAGGAAAAAC
    AGAAATTGTT CTCAGCTCAT AGCTGCATAT AAGGATTTTT GTGAGCATGG AACAAAGTCT
    GGGTTAAACC AGGGGGCCAT TTCTACTCTT CAAAGTAGTG ATATTCTTAA TTTAACAAAA
    GAACAACCTC AGGCCAAAGC AGGCAATGGA CAGAACTCTT GTGGAGTAGA AGATGTCCTT
    CAGCTTCTGC GTATTCTATA TATAGTTGCA AGTGACCCTT ATTCAAGAAT ATCCCAGGAA
    GATGGTGATG AACAGCCTCA GTTTACTTTT CCACCAGATG AATTCACTAG CAAAAAAATT
    ACAACAAAAA TATTACAGCA GATTGAGGAA CCATTGGCAC TGGCAAGTGG GGCTCTGCCA
    GACTGGTGTG AACAATTAAC CAGCAAATGT CCTTTTCTAA TACCATTTGA AACTAGACAG
    CTTTATTTCA CATGTACAGC ATTTGGCGCC TCAAGAGCAA TAGTATGGTT ACAGAACCGA
    CGTGAAGCCA CTGTGGAGCG AACGAGAACC ACAAGCAGTG TTAGGCGAGA TGACCCTGGA
    GAGTTTCGAG TTGGTCGTCT CAAGCATGAA AGAGTAAAAG TTCCACGTGG CGAGTCACTG
    ATGGAATGGG CTGAGAATGT CATGCAAATA CATGCAGATC GGAAATCAGT TCTTGAGGTT
    GAATTTTTAG GAGAAGAAGG AACTGGCTTG GGACCCACAT TAGAGTTTTA TGCTCTGGTG
    GCAGCAGAAT TCCAGAGAAC TGACTTGGGA GCTTGGCTTT GTGATGATAA TTTTCCAGAT
    GATGAATCTC GTCACGTTGA TCTTGGAGGT GGATTGAAAC CTCCTGGATA TTATGTGCAG
    AGGTCATGTG GACTGTTCAC AGCACCATTT CCACAGGATA GTGATGAGCT TGAAAGGATC
    ACGAAACTGT TTCATTTCCT TGGAATTTTC TTGGCCAAAT GCATTCAAGA CAATAGACTT
    GTGGACTTAC CTATTTCTAA ACCTTTTTTT AAACTTATGT GTATGGGTGA CATTAAAAGC
    AATATGAGTA AACTGATTTA TGAGTCACGA GGTGATAGAG ACTTACACTG TACTGAAAGT
    CAGTCTGAAG CTTCTACAGA AGAAGGTCAT GATTCACTCT CGGTAGGAAG CTTTGAAGAG
    GATTCAAAAT CAGAATTTAT TCTTGATCCC CCTAAACCAA AACCCCCAGC TTGGTTTAAT
    GGAATTTTGA CTTGGGAAGA CTTTGAATTA GTAAACCCAC ACAGAGCCAG ATTTTTAAAA
    GAAATTAAAG ACCTTGCTAT CAAGAGGCGC CAAATTTTAA GCAACAAAGG TCTTTCTGAA
    GATGAGAAGA ACACAAAATT ACAGGAACTA GTGCTGAAGA ATCCATCAGG TTCTGGGCCT
    CCACTTAGCA TAGAGGATTT AGGTTTAAAT TTCCAGTTTT GCCCTTCCTC AAGAATATAT
    GGTTTTACAG CTGTGGATCT CAAGCCAAGT GGTGAAGATG AGATGATAAC AATGGATAAT
    GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTTTGTATGC ATACGGGTAT TCAGAAACAA
    ATGGAAGCCT TTAGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC
    AGCCATGAAG AAGTCCAAAT GATTCTTTGT GGAAACCAGT CACCATCCTG GGCAGCAGAG
    GATATTATCA ATTACACTGA ACCTAAGCTG GGTTATACAC GTGACAGCCC TGGTTTCCTG
    AGGTTTGTGA GGGTTTTATG TGGCATGTCT TCTGATGAAA GGAAAGCATT CTTGCAGTTT
    ACCACTGGTT GTTCAACTCT ACCCCCAGGT GGACTGGCTA ACCTGCATCC CAGGCTCACG
    GTTGTACGCA AGGTTGATGC TACTGATGCA AGCTATCCAT CAGTCAATAC ATGTGTGCAT
    TACCTTAAGT TGCCTGAATA TTCTTCCGAG GAGATCATGA GAGAGCGCCT GCTAGCTGCT
    ACAATGGAGA AAGGCTTTCA TCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876641.1
    CDS724..7713
    Translation

    Target ORF information:

    RefSeq Version XM_017021152.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017021152.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCTG GTACTACATC AACAGTGACT ATGTCAACAT CCAGTGTTAC TAGCAGCAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTTGGTCAAT CTTTAAGTAA CACTTTAACC
    ACCAGCCTCA CATCAACTTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTATTGG CTGAGCTCGA TGATGATGAG
    GACTTACCTG AGCCAGATGA AGAAGATGAT GAGAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGATTCTGAG ACGCCCATCC CTGCAACGTC GAGCTGGCTC CCGCTCTGAT
    GTAACGCATC ATGCTGTTAC CTCGCAGCTA CCACAGGTAC CTGCTGGAGC AGGGAGCCGA
    CCTATTGGGG AGCAGGAAGA AGAAGAGTAC GAAACTAAAG GAGGACGCCG GAGAACATGG
    GATGATGATT ATGTGCTAAA GAGACAGTTT TCTGCATTGG TTCCTGCTTT TGATCCTAGA
    CCTGGTCGTA CTAATGTCCA GCAGACAACT GATCTAGAAA TACCACCCCC AGGGACCCCT
    CATTCAGAGC TCTTGGAAGA AGTCGAATGT ACTCCGTCAC CTCGATTAGC TCTCACTTTG
    AAAGTAACAG GTCTTGGAAC GACTCGTGAA GTTGAATTAC CACTCACCAA TTTCAGATCA
    ACCATCTTTT ACTATGTACA AAAATTGCTT CAATTGTCCT GTAATGGCAA TGTGAAATCA
    GATAAACTTA GGCGTATTTG GGAGCCCACA TACACAATCA TGTACAGAGA AATGAAGGAT
    TCTGATAAAG AAAAGGAAAA TGGAAAAATG GGTTGCTGGT CTATAGAGCA TGTGGAGCAG
    TACCTTGGCA CTGATGAATT ACCAAAGAAT GACTTGATAA CCTACCTGCA GAAGAATGCA
    GACGCTGCTT TCCTGCGCCA CTGGAAATTA ACTGGCACTA ATAAAAGTAT TAGGAAAAAC
    AGAAATTGTT CTCAGCTCAT AGCTGCATAT AAGGATTTTT GTGAGCATGG AACAAAGTCT
    GGGTTAAACC AGGGGGCCAT TTCTACTCTT CAAAGTAGTG ATATTCTTAA TTTAACAAAA
    GAACAACCTC AGGCCAAAGC AGGCAATGGA CAGAACTCTT GTGGAGTAGA AGATGTCCTT
    CAGCTTCTGC GTATTCTATA TATAGTTGCA AGTGACCCTT ATTCAAGAAT ATCCCAGGAA
    GATGGTGATG AACAGCCTCA GTTTACTTTT CCACCAGATG AATTCACTAG CAAAAAAATT
    ACAACAAAAA TATTACAGCA GATTGAGGAA CCATTGGCAC TGGCAAGTGG GGCTCTGCCA
    GACTGGTGTG AACAATTAAC CAGCAAATGT CCTTTTCTAA TACCATTTGA AACTAGACAG
    CTTTATTTCA CATGTACAGC ATTTGGCGCC TCAAGAGCAA TAGTATGGTT ACAGAACCGA
    CGTGAAGCCA CTGTGGAGCG AACGAGAACC ACAAGCAGTG TTAGGCGAGA TGACCCTGGA
    GAGTTTCGAG TTGGTCGTCT CAAGCATGAA AGAGTAAAAG TTCCACGTGG CGAGTCACTG
    ATGGAATGGG CTGAGAATGT CATGCAAATA CATGCAGATC GGAAATCAGT TCTTGAGGTT
    GAATTTTTAG GAGAAGAAGG AACTGGCTTG GGACCCACAT TAGAGTTTTA TGCTCTGGTG
    GCAGCAGAAT TCCAGAGAAC TGACTTGGGA GCTTGGCTTT GTGATGATAA TTTTCCAGAT
    GATGAATCTC GTCACGTTGA TCTTGGAGGT GGATTGAAAC CTCCTGGATA TTATGTGCAG
    AGGTCATGTG GACTGTTCAC AGCACCATTT CCACAGGATA GTGATGAGCT TGAAAGGATC
    ACGAAACTGT TTCATTTCCT TGGAATTTTC TTGGCCAAAT GCATTCAAGA CAATAGACTT
    GTGGACTTAC CTATTTCTAA ACCTTTTTTT AAACTTATGT GTATGGGTGA CATTAAAAGC
    AATATGAGTA AACTGATTTA TGAGTCACGA GGTGATAGAG ACTTACACTG TACTGAAAGT
    CAGTCTGAAG CTTCTACAGA AGAAGGTCAT GATTCACTCT CGGTAGGAAG CTTTGAAGAG
    GATTCAAAAT CAGAATTTAT TCTTGATCCC CCTAAACCAA AACCCCCAGC TTGGTTTAAT
    GGAATTTTGA CTTGGGAAGA CTTTGAATTA GTAAACCCAC ACAGAGCCAG ATTTTTAAAA
    GAAATTAAAG ACCTTGCTAT CAAGAGGCGC CAAATTTTAA GCAACAAAGG TCTTTCTGAA
    GATGAGAAGA ACACAAAATT ACAGGAACTA GTGCTGAAGA ATCCATCAGG TTCTGGGCCT
    CCACTTAGCA TAGAGGATTT AGGTTTAAAT TTCCAGTTTT GCCCTTCCTC AAGAATATAT
    GGTTTTACAG CTGTGGATCT CAAGCCAAGT GGTGAAGATG AGATGATAAC AATGGATAAT
    GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTTTGTATGC ATACGGGTAT TCAGAAACAA
    ATGGAAGCCT TTAGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC
    AGCCATGAAG AAGTCCAAAT GATTCTTTGT GGAAACCAGT CACCATCCTG GGCAGCAGAG
    GATATTATCA ATTACACTGA ACCTAAGCTG GGTTATACAC GTGACAGCCC TGGTTTCCTG
    AGGTTTGTGA GGGTTTTATG TGGCATGTCT TCTGATGAAA GGAAAGCATT CTTGCAGTTT
    ACCACTGGTT GTTCAACTCT ACCCCCAGGT GGACTGGCTA ACCTGCATCC CAGGCTCACG
    GTTGTACGCA AGGTTGATGC TACTGATGCA AGCTATCCAT CAGTCAATAC ATGTGTGCAT
    TACCTTAAGT TGCCTGAATA TTCTTCCGAG GAGATCATGA GAGAGCGCCT GCTAGCTGCT
    ACAATGGAGA AAGGCTTTCA TCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu84856
    Clone ID Related Accession (Same CDS sequence) XM_017021153.1 , XM_017021152.1 , XM_017021152.2
    Accession Version XM_017021152.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6990bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017021152.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCTG GTACTACATC AACAGTGACT ATGTCAACAT CCAGTGTTAC TAGCAGCAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTTGGTCAAT CTTTAAGTAA CACTTTAACC
    ACCAGCCTCA CATCAACTTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTATTGG CTGAGCTCGA TGATGATGAG
    GACTTACCTG AGCCAGATGA AGAAGATGAT GAGAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGATTCTGAG ACGCCCATCC CTGCAACGTC GAGCTGGCTC CCGCTCTGAT
    GTAACGCATC ATGCTGTTAC CTCGCAGCTA CCACAGGTAC CTGCTGGAGC AGGGAGCCGA
    CCTATTGGGG AGCAGGAAGA AGAAGAGTAC GAAACTAAAG GAGGACGCCG GAGAACATGG
    GATGATGATT ATGTGCTAAA GAGACAGTTT TCTGCATTGG TTCCTGCTTT TGATCCTAGA
    CCTGGTCGTA CTAATGTCCA GCAGACAACT GATCTAGAAA TACCACCCCC AGGGACCCCT
    CATTCAGAGC TCTTGGAAGA AGTCGAATGT ACTCCGTCAC CTCGATTAGC TCTCACTTTG
    AAAGTAACAG GTCTTGGAAC GACTCGTGAA GTTGAATTAC CACTCACCAA TTTCAGATCA
    ACCATCTTTT ACTATGTACA AAAATTGCTT CAATTGTCCT GTAATGGCAA TGTGAAATCA
    GATAAACTTA GGCGTATTTG GGAGCCCACA TACACAATCA TGTACAGAGA AATGAAGGAT
    TCTGATAAAG AAAAGGAAAA TGGAAAAATG GGTTGCTGGT CTATAGAGCA TGTGGAGCAG
    TACCTTGGCA CTGATGAATT ACCAAAGAAT GACTTGATAA CCTACCTGCA GAAGAATGCA
    GACGCTGCTT TCCTGCGCCA CTGGAAATTA ACTGGCACTA ATAAAAGTAT TAGGAAAAAC
    AGAAATTGTT CTCAGCTCAT AGCTGCATAT AAGGATTTTT GTGAGCATGG AACAAAGTCT
    GGGTTAAACC AGGGGGCCAT TTCTACTCTT CAAAGTAGTG ATATTCTTAA TTTAACAAAA
    GAACAACCTC AGGCCAAAGC AGGCAATGGA CAGAACTCTT GTGGAGTAGA AGATGTCCTT
    CAGCTTCTGC GTATTCTATA TATAGTTGCA AGTGACCCTT ATTCAAGAAT ATCCCAGGAA
    GATGGTGATG AACAGCCTCA GTTTACTTTT CCACCAGATG AATTCACTAG CAAAAAAATT
    ACAACAAAAA TATTACAGCA GATTGAGGAA CCATTGGCAC TGGCAAGTGG GGCTCTGCCA
    GACTGGTGTG AACAATTAAC CAGCAAATGT CCTTTTCTAA TACCATTTGA AACTAGACAG
    CTTTATTTCA CATGTACAGC ATTTGGCGCC TCAAGAGCAA TAGTATGGTT ACAGAACCGA
    CGTGAAGCCA CTGTGGAGCG AACGAGAACC ACAAGCAGTG TTAGGCGAGA TGACCCTGGA
    GAGTTTCGAG TTGGTCGTCT CAAGCATGAA AGAGTAAAAG TTCCACGTGG CGAGTCACTG
    ATGGAATGGG CTGAGAATGT CATGCAAATA CATGCAGATC GGAAATCAGT TCTTGAGGTT
    GAATTTTTAG GAGAAGAAGG AACTGGCTTG GGACCCACAT TAGAGTTTTA TGCTCTGGTG
    GCAGCAGAAT TCCAGAGAAC TGACTTGGGA GCTTGGCTTT GTGATGATAA TTTTCCAGAT
    GATGAATCTC GTCACGTTGA TCTTGGAGGT GGATTGAAAC CTCCTGGATA TTATGTGCAG
    AGGTCATGTG GACTGTTCAC AGCACCATTT CCACAGGATA GTGATGAGCT TGAAAGGATC
    ACGAAACTGT TTCATTTCCT TGGAATTTTC TTGGCCAAAT GCATTCAAGA CAATAGACTT
    GTGGACTTAC CTATTTCTAA ACCTTTTTTT AAACTTATGT GTATGGGTGA CATTAAAAGC
    AATATGAGTA AACTGATTTA TGAGTCACGA GGTGATAGAG ACTTACACTG TACTGAAAGT
    CAGTCTGAAG CTTCTACAGA AGAAGGTCAT GATTCACTCT CGGTAGGAAG CTTTGAAGAG
    GATTCAAAAT CAGAATTTAT TCTTGATCCC CCTAAACCAA AACCCCCAGC TTGGTTTAAT
    GGAATTTTGA CTTGGGAAGA CTTTGAATTA GTAAACCCAC ACAGAGCCAG ATTTTTAAAA
    GAAATTAAAG ACCTTGCTAT CAAGAGGCGC CAAATTTTAA GCAACAAAGG TCTTTCTGAA
    GATGAGAAGA ACACAAAATT ACAGGAACTA GTGCTGAAGA ATCCATCAGG TTCTGGGCCT
    CCACTTAGCA TAGAGGATTT AGGTTTAAAT TTCCAGTTTT GCCCTTCCTC AAGAATATAT
    GGTTTTACAG CTGTGGATCT CAAGCCAAGT GGTGAAGATG AGATGATAAC AATGGATAAT
    GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTTTGTATGC ATACGGGTAT TCAGAAACAA
    ATGGAAGCCT TTAGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC
    AGCCATGAAG AAGTCCAAAT GATTCTTTGT GGAAACCAGT CACCATCCTG GGCAGCAGAG
    GATATTATCA ATTACACTGA ACCTAAGCTG GGTTATACAC GTGACAGCCC TGGTTTCCTG
    AGGTTTGTGA GGGTTTTATG TGGCATGTCT TCTGATGAAA GGAAAGCATT CTTGCAGTTT
    ACCACTGGTT GTTCAACTCT ACCCCCAGGT GGACTGGCTA ACCTGCATCC CAGGCTCACG
    GTTGTACGCA AGGTTGATGC TACTGATGCA AGCTATCCAT CAGTCAATAC ATGTGTGCAT
    TACCTTAAGT TGCCTGAATA TTCTTCCGAG GAGATCATGA GAGAGCGCCT GCTAGCTGCT
    ACAATGGAGA AAGGCTTTCA TCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876641.1
    CDS530..7519
    Translation

    Target ORF information:

    RefSeq Version XM_017021152.2
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017021152.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCTG GTACTACATC AACAGTGACT ATGTCAACAT CCAGTGTTAC TAGCAGCAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTTGGTCAAT CTTTAAGTAA CACTTTAACC
    ACCAGCCTCA CATCAACTTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTATTGG CTGAGCTCGA TGATGATGAG
    GACTTACCTG AGCCAGATGA AGAAGATGAT GAGAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGATTCTGAG ACGCCCATCC CTGCAACGTC GAGCTGGCTC CCGCTCTGAT
    GTAACGCATC ATGCTGTTAC CTCGCAGCTA CCACAGGTAC CTGCTGGAGC AGGGAGCCGA
    CCTATTGGGG AGCAGGAAGA AGAAGAGTAC GAAACTAAAG GAGGACGCCG GAGAACATGG
    GATGATGATT ATGTGCTAAA GAGACAGTTT TCTGCATTGG TTCCTGCTTT TGATCCTAGA
    CCTGGTCGTA CTAATGTCCA GCAGACAACT GATCTAGAAA TACCACCCCC AGGGACCCCT
    CATTCAGAGC TCTTGGAAGA AGTCGAATGT ACTCCGTCAC CTCGATTAGC TCTCACTTTG
    AAAGTAACAG GTCTTGGAAC GACTCGTGAA GTTGAATTAC CACTCACCAA TTTCAGATCA
    ACCATCTTTT ACTATGTACA AAAATTGCTT CAATTGTCCT GTAATGGCAA TGTGAAATCA
    GATAAACTTA GGCGTATTTG GGAGCCCACA TACACAATCA TGTACAGAGA AATGAAGGAT
    TCTGATAAAG AAAAGGAAAA TGGAAAAATG GGTTGCTGGT CTATAGAGCA TGTGGAGCAG
    TACCTTGGCA CTGATGAATT ACCAAAGAAT GACTTGATAA CCTACCTGCA GAAGAATGCA
    GACGCTGCTT TCCTGCGCCA CTGGAAATTA ACTGGCACTA ATAAAAGTAT TAGGAAAAAC
    AGAAATTGTT CTCAGCTCAT AGCTGCATAT AAGGATTTTT GTGAGCATGG AACAAAGTCT
    GGGTTAAACC AGGGGGCCAT TTCTACTCTT CAAAGTAGTG ATATTCTTAA TTTAACAAAA
    GAACAACCTC AGGCCAAAGC AGGCAATGGA CAGAACTCTT GTGGAGTAGA AGATGTCCTT
    CAGCTTCTGC GTATTCTATA TATAGTTGCA AGTGACCCTT ATTCAAGAAT ATCCCAGGAA
    GATGGTGATG AACAGCCTCA GTTTACTTTT CCACCAGATG AATTCACTAG CAAAAAAATT
    ACAACAAAAA TATTACAGCA GATTGAGGAA CCATTGGCAC TGGCAAGTGG GGCTCTGCCA
    GACTGGTGTG AACAATTAAC CAGCAAATGT CCTTTTCTAA TACCATTTGA AACTAGACAG
    CTTTATTTCA CATGTACAGC ATTTGGCGCC TCAAGAGCAA TAGTATGGTT ACAGAACCGA
    CGTGAAGCCA CTGTGGAGCG AACGAGAACC ACAAGCAGTG TTAGGCGAGA TGACCCTGGA
    GAGTTTCGAG TTGGTCGTCT CAAGCATGAA AGAGTAAAAG TTCCACGTGG CGAGTCACTG
    ATGGAATGGG CTGAGAATGT CATGCAAATA CATGCAGATC GGAAATCAGT TCTTGAGGTT
    GAATTTTTAG GAGAAGAAGG AACTGGCTTG GGACCCACAT TAGAGTTTTA TGCTCTGGTG
    GCAGCAGAAT TCCAGAGAAC TGACTTGGGA GCTTGGCTTT GTGATGATAA TTTTCCAGAT
    GATGAATCTC GTCACGTTGA TCTTGGAGGT GGATTGAAAC CTCCTGGATA TTATGTGCAG
    AGGTCATGTG GACTGTTCAC AGCACCATTT CCACAGGATA GTGATGAGCT TGAAAGGATC
    ACGAAACTGT TTCATTTCCT TGGAATTTTC TTGGCCAAAT GCATTCAAGA CAATAGACTT
    GTGGACTTAC CTATTTCTAA ACCTTTTTTT AAACTTATGT GTATGGGTGA CATTAAAAGC
    AATATGAGTA AACTGATTTA TGAGTCACGA GGTGATAGAG ACTTACACTG TACTGAAAGT
    CAGTCTGAAG CTTCTACAGA AGAAGGTCAT GATTCACTCT CGGTAGGAAG CTTTGAAGAG
    GATTCAAAAT CAGAATTTAT TCTTGATCCC CCTAAACCAA AACCCCCAGC TTGGTTTAAT
    GGAATTTTGA CTTGGGAAGA CTTTGAATTA GTAAACCCAC ACAGAGCCAG ATTTTTAAAA
    GAAATTAAAG ACCTTGCTAT CAAGAGGCGC CAAATTTTAA GCAACAAAGG TCTTTCTGAA
    GATGAGAAGA ACACAAAATT ACAGGAACTA GTGCTGAAGA ATCCATCAGG TTCTGGGCCT
    CCACTTAGCA TAGAGGATTT AGGTTTAAAT TTCCAGTTTT GCCCTTCCTC AAGAATATAT
    GGTTTTACAG CTGTGGATCT CAAGCCAAGT GGTGAAGATG AGATGATAAC AATGGATAAT
    GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTTTGTATGC ATACGGGTAT TCAGAAACAA
    ATGGAAGCCT TTAGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC
    AGCCATGAAG AAGTCCAAAT GATTCTTTGT GGAAACCAGT CACCATCCTG GGCAGCAGAG
    GATATTATCA ATTACACTGA ACCTAAGCTG GGTTATACAC GTGACAGCCC TGGTTTCCTG
    AGGTTTGTGA GGGTTTTATG TGGCATGTCT TCTGATGAAA GGAAAGCATT CTTGCAGTTT
    ACCACTGGTT GTTCAACTCT ACCCCCAGGT GGACTGGCTA ACCTGCATCC CAGGCTCACG
    GTTGTACGCA AGGTTGATGC TACTGATGCA AGCTATCCAT CAGTCAATAC ATGTGTGCAT
    TACCTTAAGT TGCCTGAATA TTCTTCCGAG GAGATCATGA GAGAGCGCCT GCTAGCTGCT
    ACAATGGAGA AAGGCTTTCA TCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16051
    Clone ID Related Accession (Same CDS sequence) XM_005267502.2 , XM_005267502.3 , NM_015382.3 , NM_015382.4
    Accession Version NM_015382.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-02
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HECTD1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA509926.1, DA818448.1, DA440215.1, AY254380.1, AB032957.2 and BM977042.1. On Apr 16, 2014 this sequence version replaced NM_015382.2. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGGAAGT TAGGCCCTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TCAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAA
    GAAGCAGCTT CACAACGACC TCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAATCTT TAAGTAACAC TTTAACCACC
    AGCCTCACAT CAACTTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTATTGGCTG AGCTCGATGA TGATGAGGAC
    TTACCTGAGC CAGATGAAGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGCCCA TCCCTGCAAC GTCGAGCTGG CTCCCGCTCT
    GATGTAACGC ATCATGCTGT TACCTCGCAG CTACCACAGG TACCTGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAAGAAGAG TACGAAACTA AAGGAGGACG CCGGAGAACA
    TGGGATGATG ATTATGTGCT AAAGAGACAG TTTTCTGCAT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GTACTAATGT CCAGCAGACA ACTGATCTAG AAATACCACC CCCAGGGACC
    CCTCATTCAG AGCTCTTGGA AGAAGTCGAA TGTACTCCGT CACCTCGATT AGCTCTCACT
    TTGAAAGTAA CAGGTCTTGG AACGACTCGT GAAGTTGAAT TACCACTCAC CAATTTCAGA
    TCAACCATCT TTTACTATGT ACAAAAATTG CTTCAATTGT CCTGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCC ACATACACAA TCATGTACAG AGAAATGAAG
    GATTCTGATA AAGAAAAGGA AAATGGAAAA ATGGGTTGCT GGTCTATAGA GCATGTGGAG
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCTG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT TAATTTAACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGACAGAACT CTTGTGGAGT AGAAGATGTC
    CTTCAGCTTC TGCGTATTCT ATATATAGTT GCAAGTGACC CTTATTCAAG AATATCCCAG
    GAAGATGGTG ATGAACAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATTACAACAA AAATATTACA GCAGATTGAG GAACCATTGG CACTGGCAAG TGGGGCTCTG
    CCAGACTGGT GTGAACAATT AACCAGCAAA TGTCCTTTTC TAATACCATT TGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGC GCCTCAAGAG CAATAGTATG GTTACAGAAC
    CGACGTGAAG CCACTGTGGA GCGAACGAGA ACCACAAGCA GTGTTAGGCG AGATGACCCT
    GGAGAGTTTC GAGTTGGTCG TCTCAAGCAT GAAAGAGTAA AAGTTCCACG TGGCGAGTCA
    CTGATGGAAT GGGCTGAGAA TGTCATGCAA ATACATGCAG ATCGGAAATC AGTTCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGC TTGGGACCCA CATTAGAGTT TTATGCTCTG
    GTGGCAGCAG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA TAATTTTCCA
    GATGATGAAT CTCGTCACGT TGATCTTGGA GGTGGATTGA AACCTCCTGG ATATTATGTG
    CAGAGGTCAT GTGGACTGTT CACAGCACCA TTTCCACAGG ATAGTGATGA GCTTGAAAGG
    ATCACGAAAC TGTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC TAAACCTTTT TTTAAACTTA TGTGTATGGG TGACATTAAA
    AGCAATATGA GTAAACTGAT TTATGAGTCA CGAGGTGATA GAGACTTACA CTGTACTGAA
    AGTCAGTCTG AAGCTTCTAC AGAAGAAGGT CATGATTCAC TCTCGGTAGG AAGCTTTGAA
    GAGGATTCAA AATCAGAATT TATTCTTGAT CCCCCTAAAC CAAAACCCCC AGCTTGGTTT
    AATGGAATTT TGACTTGGGA AGACTTTGAA TTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC TATCAAGAGG CGCCAAATTT TAAGCAACAA AGGTCTTTCT
    GAAGATGAGA AGAACACAAA ATTACAGGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGTA TGCATACGGG TATTCAGAAA
    CAAATGGAAG CCTTTAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAAGTCCA AATGATTCTT TGTGGAAACC AGTCACCATC CTGGGCAGCA
    GAGGATATTA TCAATTACAC TGAACCTAAG CTGGGTTATA CACGTGACAG CCCTGGTTTC
    CTGAGGTTTG TGAGGGTTTT ATGTGGCATG TCTTCTGATG AAAGGAAAGC ATTCTTGCAG
    TTTACCACTG GTTGTTCAAC TCTACCCCCA GGTGGACTGG CTAACCTGCA TCCCAGGCTC
    ACGGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTATC CATCAGTCAA TACATGTGTG
    CATTACCTTA AGTTGCCTGA ATATTCTTCC GAGGAGATCA TGAGAGAGCG CCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_056197.3
    CDS530..8362
    Translation

    Target ORF information:

    RefSeq Version NM_015382.3
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_015382.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGGAAGT TAGGCCCTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TCAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAA
    GAAGCAGCTT CACAACGACC TCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAATCTT TAAGTAACAC TTTAACCACC
    AGCCTCACAT CAACTTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTATTGGCTG AGCTCGATGA TGATGAGGAC
    TTACCTGAGC CAGATGAAGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGCCCA TCCCTGCAAC GTCGAGCTGG CTCCCGCTCT
    GATGTAACGC ATCATGCTGT TACCTCGCAG CTACCACAGG TACCTGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAAGAAGAG TACGAAACTA AAGGAGGACG CCGGAGAACA
    TGGGATGATG ATTATGTGCT AAAGAGACAG TTTTCTGCAT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GTACTAATGT CCAGCAGACA ACTGATCTAG AAATACCACC CCCAGGGACC
    CCTCATTCAG AGCTCTTGGA AGAAGTCGAA TGTACTCCGT CACCTCGATT AGCTCTCACT
    TTGAAAGTAA CAGGTCTTGG AACGACTCGT GAAGTTGAAT TACCACTCAC CAATTTCAGA
    TCAACCATCT TTTACTATGT ACAAAAATTG CTTCAATTGT CCTGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCC ACATACACAA TCATGTACAG AGAAATGAAG
    GATTCTGATA AAGAAAAGGA AAATGGAAAA ATGGGTTGCT GGTCTATAGA GCATGTGGAG
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCTG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT TAATTTAACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGACAGAACT CTTGTGGAGT AGAAGATGTC
    CTTCAGCTTC TGCGTATTCT ATATATAGTT GCAAGTGACC CTTATTCAAG AATATCCCAG
    GAAGATGGTG ATGAACAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATTACAACAA AAATATTACA GCAGATTGAG GAACCATTGG CACTGGCAAG TGGGGCTCTG
    CCAGACTGGT GTGAACAATT AACCAGCAAA TGTCCTTTTC TAATACCATT TGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGC GCCTCAAGAG CAATAGTATG GTTACAGAAC
    CGACGTGAAG CCACTGTGGA GCGAACGAGA ACCACAAGCA GTGTTAGGCG AGATGACCCT
    GGAGAGTTTC GAGTTGGTCG TCTCAAGCAT GAAAGAGTAA AAGTTCCACG TGGCGAGTCA
    CTGATGGAAT GGGCTGAGAA TGTCATGCAA ATACATGCAG ATCGGAAATC AGTTCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGC TTGGGACCCA CATTAGAGTT TTATGCTCTG
    GTGGCAGCAG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA TAATTTTCCA
    GATGATGAAT CTCGTCACGT TGATCTTGGA GGTGGATTGA AACCTCCTGG ATATTATGTG
    CAGAGGTCAT GTGGACTGTT CACAGCACCA TTTCCACAGG ATAGTGATGA GCTTGAAAGG
    ATCACGAAAC TGTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC TAAACCTTTT TTTAAACTTA TGTGTATGGG TGACATTAAA
    AGCAATATGA GTAAACTGAT TTATGAGTCA CGAGGTGATA GAGACTTACA CTGTACTGAA
    AGTCAGTCTG AAGCTTCTAC AGAAGAAGGT CATGATTCAC TCTCGGTAGG AAGCTTTGAA
    GAGGATTCAA AATCAGAATT TATTCTTGAT CCCCCTAAAC CAAAACCCCC AGCTTGGTTT
    AATGGAATTT TGACTTGGGA AGACTTTGAA TTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC TATCAAGAGG CGCCAAATTT TAAGCAACAA AGGTCTTTCT
    GAAGATGAGA AGAACACAAA ATTACAGGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGTA TGCATACGGG TATTCAGAAA
    CAAATGGAAG CCTTTAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAAGTCCA AATGATTCTT TGTGGAAACC AGTCACCATC CTGGGCAGCA
    GAGGATATTA TCAATTACAC TGAACCTAAG CTGGGTTATA CACGTGACAG CCCTGGTTTC
    CTGAGGTTTG TGAGGGTTTT ATGTGGCATG TCTTCTGATG AAAGGAAAGC ATTCTTGCAG
    TTTACCACTG GTTGTTCAAC TCTACCCCCA GGTGGACTGG CTAACCTGCA TCCCAGGCTC
    ACGGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTATC CATCAGTCAA TACATGTGTG
    CATTACCTTA AGTTGCCTGA ATATTCTTCC GAGGAGATCA TGAGAGAGCG CCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16051
    Clone ID Related Accession (Same CDS sequence) XM_005267502.2 , XM_005267502.3 , NM_015382.3 , NM_015382.4
    Accession Version NM_015382.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HECTD1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL136418.4, DA509926.1, DA818448.1, DA440215.1, AY254380.1, AB032957.2 and BM977042.1. On Nov 22, 2018 this sequence version replaced NM_015382.3. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.155868.1, SRR1803612.230324.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000399332.6/ ENSP00000382269.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGGAAGT TAGGCCCTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TCAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAA
    GAAGCAGCTT CACAACGACC TCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAATCTT TAAGTAACAC TTTAACCACC
    AGCCTCACAT CAACTTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTATTGGCTG AGCTCGATGA TGATGAGGAC
    TTACCTGAGC CAGATGAAGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGCCCA TCCCTGCAAC GTCGAGCTGG CTCCCGCTCT
    GATGTAACGC ATCATGCTGT TACCTCGCAG CTACCACAGG TACCTGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAAGAAGAG TACGAAACTA AAGGAGGACG CCGGAGAACA
    TGGGATGATG ATTATGTGCT AAAGAGACAG TTTTCTGCAT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GTACTAATGT CCAGCAGACA ACTGATCTAG AAATACCACC CCCAGGGACC
    CCTCATTCAG AGCTCTTGGA AGAAGTCGAA TGTACTCCGT CACCTCGATT AGCTCTCACT
    TTGAAAGTAA CAGGTCTTGG AACGACTCGT GAAGTTGAAT TACCACTCAC CAATTTCAGA
    TCAACCATCT TTTACTATGT ACAAAAATTG CTTCAATTGT CCTGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCC ACATACACAA TCATGTACAG AGAAATGAAG
    GATTCTGATA AAGAAAAGGA AAATGGAAAA ATGGGTTGCT GGTCTATAGA GCATGTGGAG
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCTG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT TAATTTAACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGACAGAACT CTTGTGGAGT AGAAGATGTC
    CTTCAGCTTC TGCGTATTCT ATATATAGTT GCAAGTGACC CTTATTCAAG AATATCCCAG
    GAAGATGGTG ATGAACAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATTACAACAA AAATATTACA GCAGATTGAG GAACCATTGG CACTGGCAAG TGGGGCTCTG
    CCAGACTGGT GTGAACAATT AACCAGCAAA TGTCCTTTTC TAATACCATT TGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGC GCCTCAAGAG CAATAGTATG GTTACAGAAC
    CGACGTGAAG CCACTGTGGA GCGAACGAGA ACCACAAGCA GTGTTAGGCG AGATGACCCT
    GGAGAGTTTC GAGTTGGTCG TCTCAAGCAT GAAAGAGTAA AAGTTCCACG TGGCGAGTCA
    CTGATGGAAT GGGCTGAGAA TGTCATGCAA ATACATGCAG ATCGGAAATC AGTTCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGC TTGGGACCCA CATTAGAGTT TTATGCTCTG
    GTGGCAGCAG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA TAATTTTCCA
    GATGATGAAT CTCGTCACGT TGATCTTGGA GGTGGATTGA AACCTCCTGG ATATTATGTG
    CAGAGGTCAT GTGGACTGTT CACAGCACCA TTTCCACAGG ATAGTGATGA GCTTGAAAGG
    ATCACGAAAC TGTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC TAAACCTTTT TTTAAACTTA TGTGTATGGG TGACATTAAA
    AGCAATATGA GTAAACTGAT TTATGAGTCA CGAGGTGATA GAGACTTACA CTGTACTGAA
    AGTCAGTCTG AAGCTTCTAC AGAAGAAGGT CATGATTCAC TCTCGGTAGG AAGCTTTGAA
    GAGGATTCAA AATCAGAATT TATTCTTGAT CCCCCTAAAC CAAAACCCCC AGCTTGGTTT
    AATGGAATTT TGACTTGGGA AGACTTTGAA TTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC TATCAAGAGG CGCCAAATTT TAAGCAACAA AGGTCTTTCT
    GAAGATGAGA AGAACACAAA ATTACAGGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGTA TGCATACGGG TATTCAGAAA
    CAAATGGAAG CCTTTAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAAGTCCA AATGATTCTT TGTGGAAACC AGTCACCATC CTGGGCAGCA
    GAGGATATTA TCAATTACAC TGAACCTAAG CTGGGTTATA CACGTGACAG CCCTGGTTTC
    CTGAGGTTTG TGAGGGTTTT ATGTGGCATG TCTTCTGATG AAAGGAAAGC ATTCTTGCAG
    TTTACCACTG GTTGTTCAAC TCTACCCCCA GGTGGACTGG CTAACCTGCA TCCCAGGCTC
    ACGGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTATC CATCAGTCAA TACATGTGTG
    CATTACCTTA AGTTGCCTGA ATATTCTTCC GAGGAGATCA TGAGAGAGCG CCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_056197.3
    CDS800..8632
    Translation

    Target ORF information:

    RefSeq Version NM_015382.4
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_015382.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGGAAGT TAGGCCCTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TCAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAA
    GAAGCAGCTT CACAACGACC TCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAATCTT TAAGTAACAC TTTAACCACC
    AGCCTCACAT CAACTTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTATTGGCTG AGCTCGATGA TGATGAGGAC
    TTACCTGAGC CAGATGAAGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGCCCA TCCCTGCAAC GTCGAGCTGG CTCCCGCTCT
    GATGTAACGC ATCATGCTGT TACCTCGCAG CTACCACAGG TACCTGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAAGAAGAG TACGAAACTA AAGGAGGACG CCGGAGAACA
    TGGGATGATG ATTATGTGCT AAAGAGACAG TTTTCTGCAT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GTACTAATGT CCAGCAGACA ACTGATCTAG AAATACCACC CCCAGGGACC
    CCTCATTCAG AGCTCTTGGA AGAAGTCGAA TGTACTCCGT CACCTCGATT AGCTCTCACT
    TTGAAAGTAA CAGGTCTTGG AACGACTCGT GAAGTTGAAT TACCACTCAC CAATTTCAGA
    TCAACCATCT TTTACTATGT ACAAAAATTG CTTCAATTGT CCTGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCC ACATACACAA TCATGTACAG AGAAATGAAG
    GATTCTGATA AAGAAAAGGA AAATGGAAAA ATGGGTTGCT GGTCTATAGA GCATGTGGAG
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCTG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT TAATTTAACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGACAGAACT CTTGTGGAGT AGAAGATGTC
    CTTCAGCTTC TGCGTATTCT ATATATAGTT GCAAGTGACC CTTATTCAAG AATATCCCAG
    GAAGATGGTG ATGAACAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATTACAACAA AAATATTACA GCAGATTGAG GAACCATTGG CACTGGCAAG TGGGGCTCTG
    CCAGACTGGT GTGAACAATT AACCAGCAAA TGTCCTTTTC TAATACCATT TGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGC GCCTCAAGAG CAATAGTATG GTTACAGAAC
    CGACGTGAAG CCACTGTGGA GCGAACGAGA ACCACAAGCA GTGTTAGGCG AGATGACCCT
    GGAGAGTTTC GAGTTGGTCG TCTCAAGCAT GAAAGAGTAA AAGTTCCACG TGGCGAGTCA
    CTGATGGAAT GGGCTGAGAA TGTCATGCAA ATACATGCAG ATCGGAAATC AGTTCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGC TTGGGACCCA CATTAGAGTT TTATGCTCTG
    GTGGCAGCAG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA TAATTTTCCA
    GATGATGAAT CTCGTCACGT TGATCTTGGA GGTGGATTGA AACCTCCTGG ATATTATGTG
    CAGAGGTCAT GTGGACTGTT CACAGCACCA TTTCCACAGG ATAGTGATGA GCTTGAAAGG
    ATCACGAAAC TGTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC TAAACCTTTT TTTAAACTTA TGTGTATGGG TGACATTAAA
    AGCAATATGA GTAAACTGAT TTATGAGTCA CGAGGTGATA GAGACTTACA CTGTACTGAA
    AGTCAGTCTG AAGCTTCTAC AGAAGAAGGT CATGATTCAC TCTCGGTAGG AAGCTTTGAA
    GAGGATTCAA AATCAGAATT TATTCTTGAT CCCCCTAAAC CAAAACCCCC AGCTTGGTTT
    AATGGAATTT TGACTTGGGA AGACTTTGAA TTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC TATCAAGAGG CGCCAAATTT TAAGCAACAA AGGTCTTTCT
    GAAGATGAGA AGAACACAAA ATTACAGGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGTA TGCATACGGG TATTCAGAAA
    CAAATGGAAG CCTTTAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAAGTCCA AATGATTCTT TGTGGAAACC AGTCACCATC CTGGGCAGCA
    GAGGATATTA TCAATTACAC TGAACCTAAG CTGGGTTATA CACGTGACAG CCCTGGTTTC
    CTGAGGTTTG TGAGGGTTTT ATGTGGCATG TCTTCTGATG AAAGGAAAGC ATTCTTGCAG
    TTTACCACTG GTTGTTCAAC TCTACCCCCA GGTGGACTGG CTAACCTGCA TCCCAGGCTC
    ACGGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTATC CATCAGTCAA TACATGTGTG
    CATTACCTTA AGTTGCCTGA ATATTCTTCC GAGGAGATCA TGAGAGAGCG CCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16051
    Clone ID Related Accession (Same CDS sequence) XM_005267502.2 , XM_005267502.3 , NM_015382.3 , NM_015382.4
    Accession Version XM_005267502.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 3, 2014 this sequence version replaced XM_005267502.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGGAAGT TAGGCCCTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TCAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAA
    GAAGCAGCTT CACAACGACC TCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAATCTT TAAGTAACAC TTTAACCACC
    AGCCTCACAT CAACTTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTATTGGCTG AGCTCGATGA TGATGAGGAC
    TTACCTGAGC CAGATGAAGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGCCCA TCCCTGCAAC GTCGAGCTGG CTCCCGCTCT
    GATGTAACGC ATCATGCTGT TACCTCGCAG CTACCACAGG TACCTGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAAGAAGAG TACGAAACTA AAGGAGGACG CCGGAGAACA
    TGGGATGATG ATTATGTGCT AAAGAGACAG TTTTCTGCAT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GTACTAATGT CCAGCAGACA ACTGATCTAG AAATACCACC CCCAGGGACC
    CCTCATTCAG AGCTCTTGGA AGAAGTCGAA TGTACTCCGT CACCTCGATT AGCTCTCACT
    TTGAAAGTAA CAGGTCTTGG AACGACTCGT GAAGTTGAAT TACCACTCAC CAATTTCAGA
    TCAACCATCT TTTACTATGT ACAAAAATTG CTTCAATTGT CCTGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCC ACATACACAA TCATGTACAG AGAAATGAAG
    GATTCTGATA AAGAAAAGGA AAATGGAAAA ATGGGTTGCT GGTCTATAGA GCATGTGGAG
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCTG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT TAATTTAACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGACAGAACT CTTGTGGAGT AGAAGATGTC
    CTTCAGCTTC TGCGTATTCT ATATATAGTT GCAAGTGACC CTTATTCAAG AATATCCCAG
    GAAGATGGTG ATGAACAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATTACAACAA AAATATTACA GCAGATTGAG GAACCATTGG CACTGGCAAG TGGGGCTCTG
    CCAGACTGGT GTGAACAATT AACCAGCAAA TGTCCTTTTC TAATACCATT TGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGC GCCTCAAGAG CAATAGTATG GTTACAGAAC
    CGACGTGAAG CCACTGTGGA GCGAACGAGA ACCACAAGCA GTGTTAGGCG AGATGACCCT
    GGAGAGTTTC GAGTTGGTCG TCTCAAGCAT GAAAGAGTAA AAGTTCCACG TGGCGAGTCA
    CTGATGGAAT GGGCTGAGAA TGTCATGCAA ATACATGCAG ATCGGAAATC AGTTCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGC TTGGGACCCA CATTAGAGTT TTATGCTCTG
    GTGGCAGCAG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA TAATTTTCCA
    GATGATGAAT CTCGTCACGT TGATCTTGGA GGTGGATTGA AACCTCCTGG ATATTATGTG
    CAGAGGTCAT GTGGACTGTT CACAGCACCA TTTCCACAGG ATAGTGATGA GCTTGAAAGG
    ATCACGAAAC TGTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC TAAACCTTTT TTTAAACTTA TGTGTATGGG TGACATTAAA
    AGCAATATGA GTAAACTGAT TTATGAGTCA CGAGGTGATA GAGACTTACA CTGTACTGAA
    AGTCAGTCTG AAGCTTCTAC AGAAGAAGGT CATGATTCAC TCTCGGTAGG AAGCTTTGAA
    GAGGATTCAA AATCAGAATT TATTCTTGAT CCCCCTAAAC CAAAACCCCC AGCTTGGTTT
    AATGGAATTT TGACTTGGGA AGACTTTGAA TTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC TATCAAGAGG CGCCAAATTT TAAGCAACAA AGGTCTTTCT
    GAAGATGAGA AGAACACAAA ATTACAGGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGTA TGCATACGGG TATTCAGAAA
    CAAATGGAAG CCTTTAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAAGTCCA AATGATTCTT TGTGGAAACC AGTCACCATC CTGGGCAGCA
    GAGGATATTA TCAATTACAC TGAACCTAAG CTGGGTTATA CACGTGACAG CCCTGGTTTC
    CTGAGGTTTG TGAGGGTTTT ATGTGGCATG TCTTCTGATG AAAGGAAAGC ATTCTTGCAG
    TTTACCACTG GTTGTTCAAC TCTACCCCCA GGTGGACTGG CTAACCTGCA TCCCAGGCTC
    ACGGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTATC CATCAGTCAA TACATGTGTG
    CATTACCTTA AGTTGCCTGA ATATTCTTCC GAGGAGATCA TGAGAGAGCG CCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005267559.2
    CDS692..8524
    Translation

    Target ORF information:

    RefSeq Version XM_005267502.2
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005267502.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGGAAGT TAGGCCCTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TCAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAA
    GAAGCAGCTT CACAACGACC TCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAATCTT TAAGTAACAC TTTAACCACC
    AGCCTCACAT CAACTTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTATTGGCTG AGCTCGATGA TGATGAGGAC
    TTACCTGAGC CAGATGAAGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGCCCA TCCCTGCAAC GTCGAGCTGG CTCCCGCTCT
    GATGTAACGC ATCATGCTGT TACCTCGCAG CTACCACAGG TACCTGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAAGAAGAG TACGAAACTA AAGGAGGACG CCGGAGAACA
    TGGGATGATG ATTATGTGCT AAAGAGACAG TTTTCTGCAT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GTACTAATGT CCAGCAGACA ACTGATCTAG AAATACCACC CCCAGGGACC
    CCTCATTCAG AGCTCTTGGA AGAAGTCGAA TGTACTCCGT CACCTCGATT AGCTCTCACT
    TTGAAAGTAA CAGGTCTTGG AACGACTCGT GAAGTTGAAT TACCACTCAC CAATTTCAGA
    TCAACCATCT TTTACTATGT ACAAAAATTG CTTCAATTGT CCTGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCC ACATACACAA TCATGTACAG AGAAATGAAG
    GATTCTGATA AAGAAAAGGA AAATGGAAAA ATGGGTTGCT GGTCTATAGA GCATGTGGAG
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCTG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT TAATTTAACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGACAGAACT CTTGTGGAGT AGAAGATGTC
    CTTCAGCTTC TGCGTATTCT ATATATAGTT GCAAGTGACC CTTATTCAAG AATATCCCAG
    GAAGATGGTG ATGAACAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATTACAACAA AAATATTACA GCAGATTGAG GAACCATTGG CACTGGCAAG TGGGGCTCTG
    CCAGACTGGT GTGAACAATT AACCAGCAAA TGTCCTTTTC TAATACCATT TGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGC GCCTCAAGAG CAATAGTATG GTTACAGAAC
    CGACGTGAAG CCACTGTGGA GCGAACGAGA ACCACAAGCA GTGTTAGGCG AGATGACCCT
    GGAGAGTTTC GAGTTGGTCG TCTCAAGCAT GAAAGAGTAA AAGTTCCACG TGGCGAGTCA
    CTGATGGAAT GGGCTGAGAA TGTCATGCAA ATACATGCAG ATCGGAAATC AGTTCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGC TTGGGACCCA CATTAGAGTT TTATGCTCTG
    GTGGCAGCAG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA TAATTTTCCA
    GATGATGAAT CTCGTCACGT TGATCTTGGA GGTGGATTGA AACCTCCTGG ATATTATGTG
    CAGAGGTCAT GTGGACTGTT CACAGCACCA TTTCCACAGG ATAGTGATGA GCTTGAAAGG
    ATCACGAAAC TGTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC TAAACCTTTT TTTAAACTTA TGTGTATGGG TGACATTAAA
    AGCAATATGA GTAAACTGAT TTATGAGTCA CGAGGTGATA GAGACTTACA CTGTACTGAA
    AGTCAGTCTG AAGCTTCTAC AGAAGAAGGT CATGATTCAC TCTCGGTAGG AAGCTTTGAA
    GAGGATTCAA AATCAGAATT TATTCTTGAT CCCCCTAAAC CAAAACCCCC AGCTTGGTTT
    AATGGAATTT TGACTTGGGA AGACTTTGAA TTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC TATCAAGAGG CGCCAAATTT TAAGCAACAA AGGTCTTTCT
    GAAGATGAGA AGAACACAAA ATTACAGGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGTA TGCATACGGG TATTCAGAAA
    CAAATGGAAG CCTTTAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAAGTCCA AATGATTCTT TGTGGAAACC AGTCACCATC CTGGGCAGCA
    GAGGATATTA TCAATTACAC TGAACCTAAG CTGGGTTATA CACGTGACAG CCCTGGTTTC
    CTGAGGTTTG TGAGGGTTTT ATGTGGCATG TCTTCTGATG AAAGGAAAGC ATTCTTGCAG
    TTTACCACTG GTTGTTCAAC TCTACCCCCA GGTGGACTGG CTAACCTGCA TCCCAGGCTC
    ACGGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTATC CATCAGTCAA TACATGTGTG
    CATTACCTTA AGTTGCCTGA ATATTCTTCC GAGGAGATCA TGAGAGAGCG CCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16051
    Clone ID Related Accession (Same CDS sequence) XM_005267502.2 , XM_005267502.3 , NM_015382.3 , NM_015382.4
    Accession Version XM_005267502.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005267502.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGGAAGT TAGGCCCTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TCAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAA
    GAAGCAGCTT CACAACGACC TCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAATCTT TAAGTAACAC TTTAACCACC
    AGCCTCACAT CAACTTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTATTGGCTG AGCTCGATGA TGATGAGGAC
    TTACCTGAGC CAGATGAAGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGCCCA TCCCTGCAAC GTCGAGCTGG CTCCCGCTCT
    GATGTAACGC ATCATGCTGT TACCTCGCAG CTACCACAGG TACCTGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAAGAAGAG TACGAAACTA AAGGAGGACG CCGGAGAACA
    TGGGATGATG ATTATGTGCT AAAGAGACAG TTTTCTGCAT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GTACTAATGT CCAGCAGACA ACTGATCTAG AAATACCACC CCCAGGGACC
    CCTCATTCAG AGCTCTTGGA AGAAGTCGAA TGTACTCCGT CACCTCGATT AGCTCTCACT
    TTGAAAGTAA CAGGTCTTGG AACGACTCGT GAAGTTGAAT TACCACTCAC CAATTTCAGA
    TCAACCATCT TTTACTATGT ACAAAAATTG CTTCAATTGT CCTGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCC ACATACACAA TCATGTACAG AGAAATGAAG
    GATTCTGATA AAGAAAAGGA AAATGGAAAA ATGGGTTGCT GGTCTATAGA GCATGTGGAG
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCTG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT TAATTTAACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGACAGAACT CTTGTGGAGT AGAAGATGTC
    CTTCAGCTTC TGCGTATTCT ATATATAGTT GCAAGTGACC CTTATTCAAG AATATCCCAG
    GAAGATGGTG ATGAACAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATTACAACAA AAATATTACA GCAGATTGAG GAACCATTGG CACTGGCAAG TGGGGCTCTG
    CCAGACTGGT GTGAACAATT AACCAGCAAA TGTCCTTTTC TAATACCATT TGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGC GCCTCAAGAG CAATAGTATG GTTACAGAAC
    CGACGTGAAG CCACTGTGGA GCGAACGAGA ACCACAAGCA GTGTTAGGCG AGATGACCCT
    GGAGAGTTTC GAGTTGGTCG TCTCAAGCAT GAAAGAGTAA AAGTTCCACG TGGCGAGTCA
    CTGATGGAAT GGGCTGAGAA TGTCATGCAA ATACATGCAG ATCGGAAATC AGTTCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGC TTGGGACCCA CATTAGAGTT TTATGCTCTG
    GTGGCAGCAG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA TAATTTTCCA
    GATGATGAAT CTCGTCACGT TGATCTTGGA GGTGGATTGA AACCTCCTGG ATATTATGTG
    CAGAGGTCAT GTGGACTGTT CACAGCACCA TTTCCACAGG ATAGTGATGA GCTTGAAAGG
    ATCACGAAAC TGTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC TAAACCTTTT TTTAAACTTA TGTGTATGGG TGACATTAAA
    AGCAATATGA GTAAACTGAT TTATGAGTCA CGAGGTGATA GAGACTTACA CTGTACTGAA
    AGTCAGTCTG AAGCTTCTAC AGAAGAAGGT CATGATTCAC TCTCGGTAGG AAGCTTTGAA
    GAGGATTCAA AATCAGAATT TATTCTTGAT CCCCCTAAAC CAAAACCCCC AGCTTGGTTT
    AATGGAATTT TGACTTGGGA AGACTTTGAA TTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC TATCAAGAGG CGCCAAATTT TAAGCAACAA AGGTCTTTCT
    GAAGATGAGA AGAACACAAA ATTACAGGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGTA TGCATACGGG TATTCAGAAA
    CAAATGGAAG CCTTTAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAAGTCCA AATGATTCTT TGTGGAAACC AGTCACCATC CTGGGCAGCA
    GAGGATATTA TCAATTACAC TGAACCTAAG CTGGGTTATA CACGTGACAG CCCTGGTTTC
    CTGAGGTTTG TGAGGGTTTT ATGTGGCATG TCTTCTGATG AAAGGAAAGC ATTCTTGCAG
    TTTACCACTG GTTGTTCAAC TCTACCCCCA GGTGGACTGG CTAACCTGCA TCCCAGGCTC
    ACGGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTATC CATCAGTCAA TACATGTGTG
    CATTACCTTA AGTTGCCTGA ATATTCTTCC GAGGAGATCA TGAGAGAGCG CCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005267559.2
    CDS376..8208
    Translation

    Target ORF information:

    RefSeq Version XM_005267502.3
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005267502.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGGAAGT TAGGCCCTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TCAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAA
    GAAGCAGCTT CACAACGACC TCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAATCTT TAAGTAACAC TTTAACCACC
    AGCCTCACAT CAACTTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTATTGGCTG AGCTCGATGA TGATGAGGAC
    TTACCTGAGC CAGATGAAGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGCCCA TCCCTGCAAC GTCGAGCTGG CTCCCGCTCT
    GATGTAACGC ATCATGCTGT TACCTCGCAG CTACCACAGG TACCTGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAAGAAGAG TACGAAACTA AAGGAGGACG CCGGAGAACA
    TGGGATGATG ATTATGTGCT AAAGAGACAG TTTTCTGCAT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GTACTAATGT CCAGCAGACA ACTGATCTAG AAATACCACC CCCAGGGACC
    CCTCATTCAG AGCTCTTGGA AGAAGTCGAA TGTACTCCGT CACCTCGATT AGCTCTCACT
    TTGAAAGTAA CAGGTCTTGG AACGACTCGT GAAGTTGAAT TACCACTCAC CAATTTCAGA
    TCAACCATCT TTTACTATGT ACAAAAATTG CTTCAATTGT CCTGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCC ACATACACAA TCATGTACAG AGAAATGAAG
    GATTCTGATA AAGAAAAGGA AAATGGAAAA ATGGGTTGCT GGTCTATAGA GCATGTGGAG
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCTG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT TAATTTAACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGACAGAACT CTTGTGGAGT AGAAGATGTC
    CTTCAGCTTC TGCGTATTCT ATATATAGTT GCAAGTGACC CTTATTCAAG AATATCCCAG
    GAAGATGGTG ATGAACAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATTACAACAA AAATATTACA GCAGATTGAG GAACCATTGG CACTGGCAAG TGGGGCTCTG
    CCAGACTGGT GTGAACAATT AACCAGCAAA TGTCCTTTTC TAATACCATT TGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGC GCCTCAAGAG CAATAGTATG GTTACAGAAC
    CGACGTGAAG CCACTGTGGA GCGAACGAGA ACCACAAGCA GTGTTAGGCG AGATGACCCT
    GGAGAGTTTC GAGTTGGTCG TCTCAAGCAT GAAAGAGTAA AAGTTCCACG TGGCGAGTCA
    CTGATGGAAT GGGCTGAGAA TGTCATGCAA ATACATGCAG ATCGGAAATC AGTTCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGC TTGGGACCCA CATTAGAGTT TTATGCTCTG
    GTGGCAGCAG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA TAATTTTCCA
    GATGATGAAT CTCGTCACGT TGATCTTGGA GGTGGATTGA AACCTCCTGG ATATTATGTG
    CAGAGGTCAT GTGGACTGTT CACAGCACCA TTTCCACAGG ATAGTGATGA GCTTGAAAGG
    ATCACGAAAC TGTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC TAAACCTTTT TTTAAACTTA TGTGTATGGG TGACATTAAA
    AGCAATATGA GTAAACTGAT TTATGAGTCA CGAGGTGATA GAGACTTACA CTGTACTGAA
    AGTCAGTCTG AAGCTTCTAC AGAAGAAGGT CATGATTCAC TCTCGGTAGG AAGCTTTGAA
    GAGGATTCAA AATCAGAATT TATTCTTGAT CCCCCTAAAC CAAAACCCCC AGCTTGGTTT
    AATGGAATTT TGACTTGGGA AGACTTTGAA TTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC TATCAAGAGG CGCCAAATTT TAAGCAACAA AGGTCTTTCT
    GAAGATGAGA AGAACACAAA ATTACAGGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGTA TGCATACGGG TATTCAGAAA
    CAAATGGAAG CCTTTAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAAGTCCA AATGATTCTT TGTGGAAACC AGTCACCATC CTGGGCAGCA
    GAGGATATTA TCAATTACAC TGAACCTAAG CTGGGTTATA CACGTGACAG CCCTGGTTTC
    CTGAGGTTTG TGAGGGTTTT ATGTGGCATG TCTTCTGATG AAAGGAAAGC ATTCTTGCAG
    TTTACCACTG GTTGTTCAAC TCTACCCCCA GGTGGACTGG CTAACCTGCA TCCCAGGCTC
    ACGGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTATC CATCAGTCAA TACATGTGTG
    CATTACCTTA AGTTGCCTGA ATATTCTTCC GAGGAGATCA TGAGAGAGCG CCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu47641
    Clone ID Related Accession (Same CDS sequence) XM_017021148.1 , XM_017021148.2
    Accession Version XM_017021148.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7692bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGC
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGGAAGTT AGGCCCTGAT
    AGTTCTGTTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT CAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAATTA ACTAATAAAG AAGCAGCTTC ACAACGACCT
    CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTGAGTTCTT TGTTGGCTGC TGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CGAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA
    GTGACTATGT CAACATCCAG TGTTACTAGC AGCAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTTG GTCAATCTTT AAGTAACACT TTAACCACCA GCCTCACATC AACTTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TATTGGCTGA GCTCGATGAT GATGAGGACT TACCTGAGCC AGATGAAGAA
    GATGATGAGA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGCCCAT CCCTGCAACG TCGAGCTGGC TCCCGCTCTG ATGTAACGCA TCATGCTGTT
    ACCTCGCAGC TACCACAGGT ACCTGCTGGA GCAGGGAGCC GACCTATTGG GGAGCAGGAA
    GAAGAAGAGT ACGAAACTAA AGGAGGACGC CGGAGAACAT GGGATGATGA TTATGTGCTA
    AAGAGACAGT TTTCTGCATT GGTTCCTGCT TTTGATCCTA GACCTGGTCG TACTAATGTC
    CAGCAGACAA CTGATCTAGA AATACCACCC CCAGGGACCC CTCATTCAGA GCTCTTGGAA
    GAAGTCGAAT GTACTCCGTC ACCTCGATTA GCTCTCACTT TGAAAGTAAC AGGTCTTGGA
    ACGACTCGTG AAGTTGAATT ACCACTCACC AATTTCAGAT CAACCATCTT TTACTATGTA
    CAAAAATTGC TTCAATTGTC CTGTAATGGC AATGTGAAAT CAGATAAACT TAGGCGTATT
    TGGGAGCCCA CATACACAAT CATGTACAGA GAAATGAAGG ATTCTGATAA AGAAAAGGAA
    AATGGAAAAA TGGGTTGCTG GTCTATAGAG CATGTGGAGC AGTACCTTGG CACTGATGAA
    TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CAGACGCTGC TTTCCTGCGC
    CACTGGAAAT TAACTGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTC
    ATAGCTGCAT ATAAGGATTT TTGTGAGCAT GGAACAAAGT CTGGGTTAAA CCAGGGGGCC
    ATTTCTACTC TTCAAAGTAG TGATATTCTT AATTTAACAA AAGAACAACC TCAGGCCAAA
    GCAGGCAATG GACAGAACTC TTGTGGAGTA GAAGATGTCC TTCAGCTTCT GCGTATTCTA
    TATATAGTTG CAAGTGACCC TTATTCAAGA ATATCCCAGG AAGATGGTGA TGAACAGCCT
    CAGTTTACTT TTCCACCAGA TGAATTCACT AGCAAAAAAA TTACAACAAA AATATTACAG
    CAGATTGAGG AACCATTGGC ACTGGCAAGT GGGGCTCTGC CAGACTGGTG TGAACAATTA
    ACCAGCAAAT GTCCTTTTCT AATACCATTT GAAACTAGAC AGCTTTATTT CACATGTACA
    GCATTTGGCG CCTCAAGAGC AATAGTATGG TTACAGAACC GACGTGAAGC CACTGTGGAG
    CGAACGAGAA CCACAAGCAG TGTTAGGCGA GATGACCCTG GAGAGTTTCG AGTTGGTCGT
    CTCAAGCATG AAAGAGTAAA AGTTCCACGT GGCGAGTCAC TGATGGAATG GGCTGAGAAT
    GTCATGCAAA TACATGCAGA TCGGAAATCA GTTCTTGAGG TTGAATTTTT AGGAGAAGAA
    GGAACTGGCT TGGGACCCAC ATTAGAGTTT TATGCTCTGG TGGCAGCAGA ATTCCAGAGA
    ACTGACTTGG GAGCTTGGCT TTGTGATGAT AATTTTCCAG ATGATGAATC TCGTCACGTT
    GATCTTGGAG GTGGATTGAA ACCTCCTGGA TATTATGTGC AGAGGTCATG TGGACTGTTC
    ACAGCACCAT TTCCACAGGA TAGTGATGAG CTTGAAAGGA TCACGAAACT GTTTCATTTC
    CTTGGAATTT TCTTGGCCAA ATGCATTCAA GACAATAGAC TTGTGGACTT ACCTATTTCT
    AAACCTTTTT TTAAACTTAT GTGTATGGGT GACATTAAAA GCAATATGAG TAAACTGATT
    TATGAGTCAC GAGGTGATAG AGACTTACAC TGTACTGAAA GTCAGTCTGA AGCTTCTACA
    GAAGAAGGTC ATGATTCACT CTCGGTAGGA AGCTTTGAAG AGGATTCAAA ATCAGAATTT
    ATTCTTGATC CCCCTAAACC AAAACCCCCA GCTTGGTTTA ATGGAATTTT GACTTGGGAA
    GACTTTGAAT TAGTAAACCC ACACAGAGCC AGATTTTTAA AAGAAATTAA AGACCTTGCT
    ATCAAGAGGC GCCAAATTTT AAGCAACAAA GGTCTTTCTG AAGATGAGAA GAACACAAAA
    TTACAGGAAC TAGTGCTGAA GAATCCATCA GGTTCTGGGC CTCCACTTAG CATAGAGGAT
    TTAGGTTTAA ATTTCCAGTT TTGCCCTTCC TCAAGAATAT ATGGTTTTAC AGCTGTGGAT
    CTCAAGCCAA GTGGTGAAGA TGAGATGATA ACAATGGATA ATGCAGAAGA ATATGTGGAT
    TTGATGTTTG ACTTTTGTAT GCATACGGGT ATTCAGAAAC AAATGGAAGC CTTTAGAGAT
    GGGTTTAATA AAGTTTTTCC AATGGAGAAA TTAAGTTCCT TCAGCCATGA AGAAGTCCAA
    ATGATTCTTT GTGGAAACCA GTCACCATCC TGGGCAGCAG AGGATATTAT CAATTACACT
    GAACCTAAGC TGGGTTATAC ACGTGACAGC CCTGGTTTCC TGAGGTTTGT GAGGGTTTTA
    TGTGGCATGT CTTCTGATGA AAGGAAAGCA TTCTTGCAGT TTACCACTGG TTGTTCAACT
    CTACCCCCAG GTGGACTGGC TAACCTGCAT CCCAGGCTCA CGGTTGTACG CAAGGTTGAT
    GCTACTGATG CAAGCTATCC ATCAGTCAAT ACATGTGTGC ATTACCTTAA GTTGCCTGAA
    TATTCTTCCG AGGAGATCAT GAGAGAGCGC CTGCTAGCTG CTACAATGGA GAAAGGCTTT
    CATCTCAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876637.1
    CDS22..7713
    Translation

    Target ORF information:

    RefSeq Version XM_017021148.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017021148.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGC
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGGAAGTT AGGCCCTGAT
    AGTTCTGTTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT CAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAATTA ACTAATAAAG AAGCAGCTTC ACAACGACCT
    CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTGAGTTCTT TGTTGGCTGC TGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CGAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA
    GTGACTATGT CAACATCCAG TGTTACTAGC AGCAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTTG GTCAATCTTT AAGTAACACT TTAACCACCA GCCTCACATC AACTTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TATTGGCTGA GCTCGATGAT GATGAGGACT TACCTGAGCC AGATGAAGAA
    GATGATGAGA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGCCCAT CCCTGCAACG TCGAGCTGGC TCCCGCTCTG ATGTAACGCA TCATGCTGTT
    ACCTCGCAGC TACCACAGGT ACCTGCTGGA GCAGGGAGCC GACCTATTGG GGAGCAGGAA
    GAAGAAGAGT ACGAAACTAA AGGAGGACGC CGGAGAACAT GGGATGATGA TTATGTGCTA
    AAGAGACAGT TTTCTGCATT GGTTCCTGCT TTTGATCCTA GACCTGGTCG TACTAATGTC
    CAGCAGACAA CTGATCTAGA AATACCACCC CCAGGGACCC CTCATTCAGA GCTCTTGGAA
    GAAGTCGAAT GTACTCCGTC ACCTCGATTA GCTCTCACTT TGAAAGTAAC AGGTCTTGGA
    ACGACTCGTG AAGTTGAATT ACCACTCACC AATTTCAGAT CAACCATCTT TTACTATGTA
    CAAAAATTGC TTCAATTGTC CTGTAATGGC AATGTGAAAT CAGATAAACT TAGGCGTATT
    TGGGAGCCCA CATACACAAT CATGTACAGA GAAATGAAGG ATTCTGATAA AGAAAAGGAA
    AATGGAAAAA TGGGTTGCTG GTCTATAGAG CATGTGGAGC AGTACCTTGG CACTGATGAA
    TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CAGACGCTGC TTTCCTGCGC
    CACTGGAAAT TAACTGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTC
    ATAGCTGCAT ATAAGGATTT TTGTGAGCAT GGAACAAAGT CTGGGTTAAA CCAGGGGGCC
    ATTTCTACTC TTCAAAGTAG TGATATTCTT AATTTAACAA AAGAACAACC TCAGGCCAAA
    GCAGGCAATG GACAGAACTC TTGTGGAGTA GAAGATGTCC TTCAGCTTCT GCGTATTCTA
    TATATAGTTG CAAGTGACCC TTATTCAAGA ATATCCCAGG AAGATGGTGA TGAACAGCCT
    CAGTTTACTT TTCCACCAGA TGAATTCACT AGCAAAAAAA TTACAACAAA AATATTACAG
    CAGATTGAGG AACCATTGGC ACTGGCAAGT GGGGCTCTGC CAGACTGGTG TGAACAATTA
    ACCAGCAAAT GTCCTTTTCT AATACCATTT GAAACTAGAC AGCTTTATTT CACATGTACA
    GCATTTGGCG CCTCAAGAGC AATAGTATGG TTACAGAACC GACGTGAAGC CACTGTGGAG
    CGAACGAGAA CCACAAGCAG TGTTAGGCGA GATGACCCTG GAGAGTTTCG AGTTGGTCGT
    CTCAAGCATG AAAGAGTAAA AGTTCCACGT GGCGAGTCAC TGATGGAATG GGCTGAGAAT
    GTCATGCAAA TACATGCAGA TCGGAAATCA GTTCTTGAGG TTGAATTTTT AGGAGAAGAA
    GGAACTGGCT TGGGACCCAC ATTAGAGTTT TATGCTCTGG TGGCAGCAGA ATTCCAGAGA
    ACTGACTTGG GAGCTTGGCT TTGTGATGAT AATTTTCCAG ATGATGAATC TCGTCACGTT
    GATCTTGGAG GTGGATTGAA ACCTCCTGGA TATTATGTGC AGAGGTCATG TGGACTGTTC
    ACAGCACCAT TTCCACAGGA TAGTGATGAG CTTGAAAGGA TCACGAAACT GTTTCATTTC
    CTTGGAATTT TCTTGGCCAA ATGCATTCAA GACAATAGAC TTGTGGACTT ACCTATTTCT
    AAACCTTTTT TTAAACTTAT GTGTATGGGT GACATTAAAA GCAATATGAG TAAACTGATT
    TATGAGTCAC GAGGTGATAG AGACTTACAC TGTACTGAAA GTCAGTCTGA AGCTTCTACA
    GAAGAAGGTC ATGATTCACT CTCGGTAGGA AGCTTTGAAG AGGATTCAAA ATCAGAATTT
    ATTCTTGATC CCCCTAAACC AAAACCCCCA GCTTGGTTTA ATGGAATTTT GACTTGGGAA
    GACTTTGAAT TAGTAAACCC ACACAGAGCC AGATTTTTAA AAGAAATTAA AGACCTTGCT
    ATCAAGAGGC GCCAAATTTT AAGCAACAAA GGTCTTTCTG AAGATGAGAA GAACACAAAA
    TTACAGGAAC TAGTGCTGAA GAATCCATCA GGTTCTGGGC CTCCACTTAG CATAGAGGAT
    TTAGGTTTAA ATTTCCAGTT TTGCCCTTCC TCAAGAATAT ATGGTTTTAC AGCTGTGGAT
    CTCAAGCCAA GTGGTGAAGA TGAGATGATA ACAATGGATA ATGCAGAAGA ATATGTGGAT
    TTGATGTTTG ACTTTTGTAT GCATACGGGT ATTCAGAAAC AAATGGAAGC CTTTAGAGAT
    GGGTTTAATA AAGTTTTTCC AATGGAGAAA TTAAGTTCCT TCAGCCATGA AGAAGTCCAA
    ATGATTCTTT GTGGAAACCA GTCACCATCC TGGGCAGCAG AGGATATTAT CAATTACACT
    GAACCTAAGC TGGGTTATAC ACGTGACAGC CCTGGTTTCC TGAGGTTTGT GAGGGTTTTA
    TGTGGCATGT CTTCTGATGA AAGGAAAGCA TTCTTGCAGT TTACCACTGG TTGTTCAACT
    CTACCCCCAG GTGGACTGGC TAACCTGCAT CCCAGGCTCA CGGTTGTACG CAAGGTTGAT
    GCTACTGATG CAAGCTATCC ATCAGTCAAT ACATGTGTGC ATTACCTTAA GTTGCCTGAA
    TATTCTTCCG AGGAGATCAT GAGAGAGCGC CTGCTAGCTG CTACAATGGA GAAAGGCTTT
    CATCTCAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu47641
    Clone ID Related Accession (Same CDS sequence) XM_017021148.1 , XM_017021148.2
    Accession Version XM_017021148.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7692bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017021148.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGC
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGGAAGTT AGGCCCTGAT
    AGTTCTGTTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT CAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAATTA ACTAATAAAG AAGCAGCTTC ACAACGACCT
    CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTGAGTTCTT TGTTGGCTGC TGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CGAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA
    GTGACTATGT CAACATCCAG TGTTACTAGC AGCAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTTG GTCAATCTTT AAGTAACACT TTAACCACCA GCCTCACATC AACTTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TATTGGCTGA GCTCGATGAT GATGAGGACT TACCTGAGCC AGATGAAGAA
    GATGATGAGA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGCCCAT CCCTGCAACG TCGAGCTGGC TCCCGCTCTG ATGTAACGCA TCATGCTGTT
    ACCTCGCAGC TACCACAGGT ACCTGCTGGA GCAGGGAGCC GACCTATTGG GGAGCAGGAA
    GAAGAAGAGT ACGAAACTAA AGGAGGACGC CGGAGAACAT GGGATGATGA TTATGTGCTA
    AAGAGACAGT TTTCTGCATT GGTTCCTGCT TTTGATCCTA GACCTGGTCG TACTAATGTC
    CAGCAGACAA CTGATCTAGA AATACCACCC CCAGGGACCC CTCATTCAGA GCTCTTGGAA
    GAAGTCGAAT GTACTCCGTC ACCTCGATTA GCTCTCACTT TGAAAGTAAC AGGTCTTGGA
    ACGACTCGTG AAGTTGAATT ACCACTCACC AATTTCAGAT CAACCATCTT TTACTATGTA
    CAAAAATTGC TTCAATTGTC CTGTAATGGC AATGTGAAAT CAGATAAACT TAGGCGTATT
    TGGGAGCCCA CATACACAAT CATGTACAGA GAAATGAAGG ATTCTGATAA AGAAAAGGAA
    AATGGAAAAA TGGGTTGCTG GTCTATAGAG CATGTGGAGC AGTACCTTGG CACTGATGAA
    TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CAGACGCTGC TTTCCTGCGC
    CACTGGAAAT TAACTGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTC
    ATAGCTGCAT ATAAGGATTT TTGTGAGCAT GGAACAAAGT CTGGGTTAAA CCAGGGGGCC
    ATTTCTACTC TTCAAAGTAG TGATATTCTT AATTTAACAA AAGAACAACC TCAGGCCAAA
    GCAGGCAATG GACAGAACTC TTGTGGAGTA GAAGATGTCC TTCAGCTTCT GCGTATTCTA
    TATATAGTTG CAAGTGACCC TTATTCAAGA ATATCCCAGG AAGATGGTGA TGAACAGCCT
    CAGTTTACTT TTCCACCAGA TGAATTCACT AGCAAAAAAA TTACAACAAA AATATTACAG
    CAGATTGAGG AACCATTGGC ACTGGCAAGT GGGGCTCTGC CAGACTGGTG TGAACAATTA
    ACCAGCAAAT GTCCTTTTCT AATACCATTT GAAACTAGAC AGCTTTATTT CACATGTACA
    GCATTTGGCG CCTCAAGAGC AATAGTATGG TTACAGAACC GACGTGAAGC CACTGTGGAG
    CGAACGAGAA CCACAAGCAG TGTTAGGCGA GATGACCCTG GAGAGTTTCG AGTTGGTCGT
    CTCAAGCATG AAAGAGTAAA AGTTCCACGT GGCGAGTCAC TGATGGAATG GGCTGAGAAT
    GTCATGCAAA TACATGCAGA TCGGAAATCA GTTCTTGAGG TTGAATTTTT AGGAGAAGAA
    GGAACTGGCT TGGGACCCAC ATTAGAGTTT TATGCTCTGG TGGCAGCAGA ATTCCAGAGA
    ACTGACTTGG GAGCTTGGCT TTGTGATGAT AATTTTCCAG ATGATGAATC TCGTCACGTT
    GATCTTGGAG GTGGATTGAA ACCTCCTGGA TATTATGTGC AGAGGTCATG TGGACTGTTC
    ACAGCACCAT TTCCACAGGA TAGTGATGAG CTTGAAAGGA TCACGAAACT GTTTCATTTC
    CTTGGAATTT TCTTGGCCAA ATGCATTCAA GACAATAGAC TTGTGGACTT ACCTATTTCT
    AAACCTTTTT TTAAACTTAT GTGTATGGGT GACATTAAAA GCAATATGAG TAAACTGATT
    TATGAGTCAC GAGGTGATAG AGACTTACAC TGTACTGAAA GTCAGTCTGA AGCTTCTACA
    GAAGAAGGTC ATGATTCACT CTCGGTAGGA AGCTTTGAAG AGGATTCAAA ATCAGAATTT
    ATTCTTGATC CCCCTAAACC AAAACCCCCA GCTTGGTTTA ATGGAATTTT GACTTGGGAA
    GACTTTGAAT TAGTAAACCC ACACAGAGCC AGATTTTTAA AAGAAATTAA AGACCTTGCT
    ATCAAGAGGC GCCAAATTTT AAGCAACAAA GGTCTTTCTG AAGATGAGAA GAACACAAAA
    TTACAGGAAC TAGTGCTGAA GAATCCATCA GGTTCTGGGC CTCCACTTAG CATAGAGGAT
    TTAGGTTTAA ATTTCCAGTT TTGCCCTTCC TCAAGAATAT ATGGTTTTAC AGCTGTGGAT
    CTCAAGCCAA GTGGTGAAGA TGAGATGATA ACAATGGATA ATGCAGAAGA ATATGTGGAT
    TTGATGTTTG ACTTTTGTAT GCATACGGGT ATTCAGAAAC AAATGGAAGC CTTTAGAGAT
    GGGTTTAATA AAGTTTTTCC AATGGAGAAA TTAAGTTCCT TCAGCCATGA AGAAGTCCAA
    ATGATTCTTT GTGGAAACCA GTCACCATCC TGGGCAGCAG AGGATATTAT CAATTACACT
    GAACCTAAGC TGGGTTATAC ACGTGACAGC CCTGGTTTCC TGAGGTTTGT GAGGGTTTTA
    TGTGGCATGT CTTCTGATGA AAGGAAAGCA TTCTTGCAGT TTACCACTGG TTGTTCAACT
    CTACCCCCAG GTGGACTGGC TAACCTGCAT CCCAGGCTCA CGGTTGTACG CAAGGTTGAT
    GCTACTGATG CAAGCTATCC ATCAGTCAAT ACATGTGTGC ATTACCTTAA GTTGCCTGAA
    TATTCTTCCG AGGAGATCAT GAGAGAGCGC CTGCTAGCTG CTACAATGGA GAAAGGCTTT
    CATCTCAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876637.1
    CDS490..8181
    Translation

    Target ORF information:

    RefSeq Version XM_017021148.2
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017021148.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGC
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGGAAGTT AGGCCCTGAT
    AGTTCTGTTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT CAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAATTA ACTAATAAAG AAGCAGCTTC ACAACGACCT
    CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTGAGTTCTT TGTTGGCTGC TGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CGAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA
    GTGACTATGT CAACATCCAG TGTTACTAGC AGCAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTTG GTCAATCTTT AAGTAACACT TTAACCACCA GCCTCACATC AACTTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TATTGGCTGA GCTCGATGAT GATGAGGACT TACCTGAGCC AGATGAAGAA
    GATGATGAGA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGCCCAT CCCTGCAACG TCGAGCTGGC TCCCGCTCTG ATGTAACGCA TCATGCTGTT
    ACCTCGCAGC TACCACAGGT ACCTGCTGGA GCAGGGAGCC GACCTATTGG GGAGCAGGAA
    GAAGAAGAGT ACGAAACTAA AGGAGGACGC CGGAGAACAT GGGATGATGA TTATGTGCTA
    AAGAGACAGT TTTCTGCATT GGTTCCTGCT TTTGATCCTA GACCTGGTCG TACTAATGTC
    CAGCAGACAA CTGATCTAGA AATACCACCC CCAGGGACCC CTCATTCAGA GCTCTTGGAA
    GAAGTCGAAT GTACTCCGTC ACCTCGATTA GCTCTCACTT TGAAAGTAAC AGGTCTTGGA
    ACGACTCGTG AAGTTGAATT ACCACTCACC AATTTCAGAT CAACCATCTT TTACTATGTA
    CAAAAATTGC TTCAATTGTC CTGTAATGGC AATGTGAAAT CAGATAAACT TAGGCGTATT
    TGGGAGCCCA CATACACAAT CATGTACAGA GAAATGAAGG ATTCTGATAA AGAAAAGGAA
    AATGGAAAAA TGGGTTGCTG GTCTATAGAG CATGTGGAGC AGTACCTTGG CACTGATGAA
    TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CAGACGCTGC TTTCCTGCGC
    CACTGGAAAT TAACTGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTC
    ATAGCTGCAT ATAAGGATTT TTGTGAGCAT GGAACAAAGT CTGGGTTAAA CCAGGGGGCC
    ATTTCTACTC TTCAAAGTAG TGATATTCTT AATTTAACAA AAGAACAACC TCAGGCCAAA
    GCAGGCAATG GACAGAACTC TTGTGGAGTA GAAGATGTCC TTCAGCTTCT GCGTATTCTA
    TATATAGTTG CAAGTGACCC TTATTCAAGA ATATCCCAGG AAGATGGTGA TGAACAGCCT
    CAGTTTACTT TTCCACCAGA TGAATTCACT AGCAAAAAAA TTACAACAAA AATATTACAG
    CAGATTGAGG AACCATTGGC ACTGGCAAGT GGGGCTCTGC CAGACTGGTG TGAACAATTA
    ACCAGCAAAT GTCCTTTTCT AATACCATTT GAAACTAGAC AGCTTTATTT CACATGTACA
    GCATTTGGCG CCTCAAGAGC AATAGTATGG TTACAGAACC GACGTGAAGC CACTGTGGAG
    CGAACGAGAA CCACAAGCAG TGTTAGGCGA GATGACCCTG GAGAGTTTCG AGTTGGTCGT
    CTCAAGCATG AAAGAGTAAA AGTTCCACGT GGCGAGTCAC TGATGGAATG GGCTGAGAAT
    GTCATGCAAA TACATGCAGA TCGGAAATCA GTTCTTGAGG TTGAATTTTT AGGAGAAGAA
    GGAACTGGCT TGGGACCCAC ATTAGAGTTT TATGCTCTGG TGGCAGCAGA ATTCCAGAGA
    ACTGACTTGG GAGCTTGGCT TTGTGATGAT AATTTTCCAG ATGATGAATC TCGTCACGTT
    GATCTTGGAG GTGGATTGAA ACCTCCTGGA TATTATGTGC AGAGGTCATG TGGACTGTTC
    ACAGCACCAT TTCCACAGGA TAGTGATGAG CTTGAAAGGA TCACGAAACT GTTTCATTTC
    CTTGGAATTT TCTTGGCCAA ATGCATTCAA GACAATAGAC TTGTGGACTT ACCTATTTCT
    AAACCTTTTT TTAAACTTAT GTGTATGGGT GACATTAAAA GCAATATGAG TAAACTGATT
    TATGAGTCAC GAGGTGATAG AGACTTACAC TGTACTGAAA GTCAGTCTGA AGCTTCTACA
    GAAGAAGGTC ATGATTCACT CTCGGTAGGA AGCTTTGAAG AGGATTCAAA ATCAGAATTT
    ATTCTTGATC CCCCTAAACC AAAACCCCCA GCTTGGTTTA ATGGAATTTT GACTTGGGAA
    GACTTTGAAT TAGTAAACCC ACACAGAGCC AGATTTTTAA AAGAAATTAA AGACCTTGCT
    ATCAAGAGGC GCCAAATTTT AAGCAACAAA GGTCTTTCTG AAGATGAGAA GAACACAAAA
    TTACAGGAAC TAGTGCTGAA GAATCCATCA GGTTCTGGGC CTCCACTTAG CATAGAGGAT
    TTAGGTTTAA ATTTCCAGTT TTGCCCTTCC TCAAGAATAT ATGGTTTTAC AGCTGTGGAT
    CTCAAGCCAA GTGGTGAAGA TGAGATGATA ACAATGGATA ATGCAGAAGA ATATGTGGAT
    TTGATGTTTG ACTTTTGTAT GCATACGGGT ATTCAGAAAC AAATGGAAGC CTTTAGAGAT
    GGGTTTAATA AAGTTTTTCC AATGGAGAAA TTAAGTTCCT TCAGCCATGA AGAAGTCCAA
    ATGATTCTTT GTGGAAACCA GTCACCATCC TGGGCAGCAG AGGATATTAT CAATTACACT
    GAACCTAAGC TGGGTTATAC ACGTGACAGC CCTGGTTTCC TGAGGTTTGT GAGGGTTTTA
    TGTGGCATGT CTTCTGATGA AAGGAAAGCA TTCTTGCAGT TTACCACTGG TTGTTCAACT
    CTACCCCCAG GTGGACTGGC TAACCTGCAT CCCAGGCTCA CGGTTGTACG CAAGGTTGAT
    GCTACTGATG CAAGCTATCC ATCAGTCAAT ACATGTGTGC ATTACCTTAA GTTGCCTGAA
    TATTCTTCCG AGGAGATCAT GAGAGAGCGC CTGCTAGCTG CTACAATGGA GAAAGGCTTT
    CATCTCAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu66519
    Clone ID Related Accession (Same CDS sequence) XM_011536619.2 , XM_024449526.1
    Accession Version XM_011536619.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011536619.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGGAAGT TAGGCCCTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TCAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAA
    GAAGCAGCTT CACAACGACC TCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAATCTT TAAGTAACAC TTTAACCACC
    AGCCTCACAT CAACTTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTATTGGCTG AGCTCGATGA TGATGAGGAC
    TTACCTGAGC CAGATGAAGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGA TTCTGAGACG CCCATCCCTG CAACGTCGAG CTGGCTCCCG CTCTGATGTA
    ACGCATCATG CTGTTACCTC GCAGCTACCA CAGGTACCTG CTGGAGCAGG GAGCCGACCT
    ATTGGGGAGC AGGAAGAAGA AGAGTACGAA ACTAAAGGAG GACGCCGGAG AACATGGGAT
    GATGATTATG TGCTAAAGAG ACAGTTTTCT GCATTGGTTC CTGCTTTTGA TCCTAGACCT
    GGTCGTACTA ATGTCCAGCA GACAACTGAT CTAGAAATAC CACCCCCAGG GACCCCTCAT
    TCAGAGCTCT TGGAAGAAGT CGAATGTACT CCGTCACCTC GATTAGCTCT CACTTTGAAA
    GTAACAGGTC TTGGAACGAC TCGTGAAGTT GAATTACCAC TCACCAATTT CAGATCAACC
    ATCTTTTACT ATGTACAAAA ATTGCTTCAA TTGTCCTGTA ATGGCAATGT GAAATCAGAT
    AAACTTAGGC GTATTTGGGA GCCCACATAC ACAATCATGT ACAGAGAAAT GAAGGATTCT
    GATAAAGAAA AGGAAAATGG AAAAATGGGT TGCTGGTCTA TAGAGCATGT GGAGCAGTAC
    CTTGGCACTG ATGAATTACC AAAGAATGAC TTGATAACCT ACCTGCAGAA GAATGCAGAC
    GCTGCTTTCC TGCGCCACTG GAAATTAACT GGCACTAATA AAAGTATTAG GAAAAACAGA
    AATTGTTCTC AGCTCATAGC TGCATATAAG GATTTTTGTG AGCATGGAAC AAAGTCTGGG
    TTAAACCAGG GGGCCATTTC TACTCTTCAA AGTAGTGATA TTCTTAATTT AACAAAAGAA
    CAACCTCAGG CCAAAGCAGG CAATGGACAG AACTCTTGTG GAGTAGAAGA TGTCCTTCAG
    CTTCTGCGTA TTCTATATAT AGTTGCAAGT GACCCTTATT CAAGAATATC CCAGGAAGAT
    GGTGATGAAC AGCCTCAGTT TACTTTTCCA CCAGATGAAT TCACTAGCAA AAAAATTACA
    ACAAAAATAT TACAGCAGAT TGAGGAACCA TTGGCACTGG CAAGTGGGGC TCTGCCAGAC
    TGGTGTGAAC AATTAACCAG CAAATGTCCT TTTCTAATAC CATTTGAAAC TAGACAGCTT
    TATTTCACAT GTACAGCATT TGGCGCCTCA AGAGCAATAG TATGGTTACA GAACCGACGT
    GAAGCCACTG TGGAGCGAAC GAGAACCACA AGCAGTGTTA GGCGAGATGA CCCTGGAGAG
    TTTCGAGTTG GTCGTCTCAA GCATGAAAGA GTAAAAGTTC CACGTGGCGA GTCACTGATG
    GAATGGGCTG AGAATGTCAT GCAAATACAT GCAGATCGGA AATCAGTTCT TGAGGTTGAA
    TTTTTAGGAG AAGAAGGAAC TGGCTTGGGA CCCACATTAG AGTTTTATGC TCTGGTGGCA
    GCAGAATTCC AGAGAACTGA CTTGGGAGCT TGGCTTTGTG ATGATAATTT TCCAGATGAT
    GAATCTCGTC ACGTTGATCT TGGAGGTGGA TTGAAACCTC CTGGATATTA TGTGCAGAGG
    TCATGTGGAC TGTTCACAGC ACCATTTCCA CAGGATAGTG ATGAGCTTGA AAGGATCACG
    AAACTGTTTC ATTTCCTTGG AATTTTCTTG GCCAAATGCA TTCAAGACAA TAGACTTGTG
    GACTTACCTA TTTCTAAACC TTTTTTTAAA CTTATGTGTA TGGGTGACAT TAAAAGCAAT
    ATGAGTAAAC TGATTTATGA GTCACGAGGT GATAGAGACT TACACTGTAC TGAAAGTCAG
    TCTGAAGCTT CTACAGAAGA AGGTCATGAT TCACTCTCGG TAGGAAGCTT TGAAGAGGAT
    TCAAAATCAG AATTTATTCT TGATCCCCCT AAACCAAAAC CCCCAGCTTG GTTTAATGGA
    ATTTTGACTT GGGAAGACTT TGAATTAGTA AACCCACACA GAGCCAGATT TTTAAAAGAA
    ATTAAAGACC TTGCTATCAA GAGGCGCCAA ATTTTAAGCA ACAAAGGTCT TTCTGAAGAT
    GAGAAGAACA CAAAATTACA GGAACTAGTG CTGAAGAATC CATCAGGTTC TGGGCCTCCA
    CTTAGCATAG AGGATTTAGG TTTAAATTTC CAGTTTTGCC CTTCCTCAAG AATATATGGT
    TTTACAGCTG TGGATCTCAA GCCAAGTGGT GAAGATGAGA TGATAACAAT GGATAATGCA
    GAAGAATATG TGGATTTGAT GTTTGACTTT TGTATGCATA CGGGTATTCA GAAACAAATG
    GAAGCCTTTA GAGATGGGTT TAATAAAGTT TTTCCAATGG AGAAATTAAG TTCCTTCAGC
    CATGAAGAAG TCCAAATGAT TCTTTGTGGA AACCAGTCAC CATCCTGGGC AGCAGAGGAT
    ATTATCAATT ACACTGAACC TAAGCTGGGT TATACACGTG ACAGCCCTGG TTTCCTGAGG
    TTTGTGAGGG TTTTATGTGG CATGTCTTCT GATGAAAGGA AAGCATTCTT GCAGTTTACC
    ACTGGTTGTT CAACTCTACC CCCAGGTGGA CTGGCTAACC TGCATCCCAG GCTCACGGTT
    GTACGCAAGG TTGATGCTAC TGATGCAAGC TATCCATCAG TCAATACATG TGTGCATTAC
    CTTAAGTTGC CTGAATATTC TTCCGAGGAG ATCATGAGAG AGCGCCTGCT AGCTGCTACA
    ATGGAGAAAG GCTTTCATCT CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011534921.1
    CDS128..7954
    Translation

    Target ORF information:

    RefSeq Version XM_011536619.2
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011536619.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGGAAGT TAGGCCCTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TCAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAA
    GAAGCAGCTT CACAACGACC TCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAATCTT TAAGTAACAC TTTAACCACC
    AGCCTCACAT CAACTTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTATTGGCTG AGCTCGATGA TGATGAGGAC
    TTACCTGAGC CAGATGAAGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGA TTCTGAGACG CCCATCCCTG CAACGTCGAG CTGGCTCCCG CTCTGATGTA
    ACGCATCATG CTGTTACCTC GCAGCTACCA CAGGTACCTG CTGGAGCAGG GAGCCGACCT
    ATTGGGGAGC AGGAAGAAGA AGAGTACGAA ACTAAAGGAG GACGCCGGAG AACATGGGAT
    GATGATTATG TGCTAAAGAG ACAGTTTTCT GCATTGGTTC CTGCTTTTGA TCCTAGACCT
    GGTCGTACTA ATGTCCAGCA GACAACTGAT CTAGAAATAC CACCCCCAGG GACCCCTCAT
    TCAGAGCTCT TGGAAGAAGT CGAATGTACT CCGTCACCTC GATTAGCTCT CACTTTGAAA
    GTAACAGGTC TTGGAACGAC TCGTGAAGTT GAATTACCAC TCACCAATTT CAGATCAACC
    ATCTTTTACT ATGTACAAAA ATTGCTTCAA TTGTCCTGTA ATGGCAATGT GAAATCAGAT
    AAACTTAGGC GTATTTGGGA GCCCACATAC ACAATCATGT ACAGAGAAAT GAAGGATTCT
    GATAAAGAAA AGGAAAATGG AAAAATGGGT TGCTGGTCTA TAGAGCATGT GGAGCAGTAC
    CTTGGCACTG ATGAATTACC AAAGAATGAC TTGATAACCT ACCTGCAGAA GAATGCAGAC
    GCTGCTTTCC TGCGCCACTG GAAATTAACT GGCACTAATA AAAGTATTAG GAAAAACAGA
    AATTGTTCTC AGCTCATAGC TGCATATAAG GATTTTTGTG AGCATGGAAC AAAGTCTGGG
    TTAAACCAGG GGGCCATTTC TACTCTTCAA AGTAGTGATA TTCTTAATTT AACAAAAGAA
    CAACCTCAGG CCAAAGCAGG CAATGGACAG AACTCTTGTG GAGTAGAAGA TGTCCTTCAG
    CTTCTGCGTA TTCTATATAT AGTTGCAAGT GACCCTTATT CAAGAATATC CCAGGAAGAT
    GGTGATGAAC AGCCTCAGTT TACTTTTCCA CCAGATGAAT TCACTAGCAA AAAAATTACA
    ACAAAAATAT TACAGCAGAT TGAGGAACCA TTGGCACTGG CAAGTGGGGC TCTGCCAGAC
    TGGTGTGAAC AATTAACCAG CAAATGTCCT TTTCTAATAC CATTTGAAAC TAGACAGCTT
    TATTTCACAT GTACAGCATT TGGCGCCTCA AGAGCAATAG TATGGTTACA GAACCGACGT
    GAAGCCACTG TGGAGCGAAC GAGAACCACA AGCAGTGTTA GGCGAGATGA CCCTGGAGAG
    TTTCGAGTTG GTCGTCTCAA GCATGAAAGA GTAAAAGTTC CACGTGGCGA GTCACTGATG
    GAATGGGCTG AGAATGTCAT GCAAATACAT GCAGATCGGA AATCAGTTCT TGAGGTTGAA
    TTTTTAGGAG AAGAAGGAAC TGGCTTGGGA CCCACATTAG AGTTTTATGC TCTGGTGGCA
    GCAGAATTCC AGAGAACTGA CTTGGGAGCT TGGCTTTGTG ATGATAATTT TCCAGATGAT
    GAATCTCGTC ACGTTGATCT TGGAGGTGGA TTGAAACCTC CTGGATATTA TGTGCAGAGG
    TCATGTGGAC TGTTCACAGC ACCATTTCCA CAGGATAGTG ATGAGCTTGA AAGGATCACG
    AAACTGTTTC ATTTCCTTGG AATTTTCTTG GCCAAATGCA TTCAAGACAA TAGACTTGTG
    GACTTACCTA TTTCTAAACC TTTTTTTAAA CTTATGTGTA TGGGTGACAT TAAAAGCAAT
    ATGAGTAAAC TGATTTATGA GTCACGAGGT GATAGAGACT TACACTGTAC TGAAAGTCAG
    TCTGAAGCTT CTACAGAAGA AGGTCATGAT TCACTCTCGG TAGGAAGCTT TGAAGAGGAT
    TCAAAATCAG AATTTATTCT TGATCCCCCT AAACCAAAAC CCCCAGCTTG GTTTAATGGA
    ATTTTGACTT GGGAAGACTT TGAATTAGTA AACCCACACA GAGCCAGATT TTTAAAAGAA
    ATTAAAGACC TTGCTATCAA GAGGCGCCAA ATTTTAAGCA ACAAAGGTCT TTCTGAAGAT
    GAGAAGAACA CAAAATTACA GGAACTAGTG CTGAAGAATC CATCAGGTTC TGGGCCTCCA
    CTTAGCATAG AGGATTTAGG TTTAAATTTC CAGTTTTGCC CTTCCTCAAG AATATATGGT
    TTTACAGCTG TGGATCTCAA GCCAAGTGGT GAAGATGAGA TGATAACAAT GGATAATGCA
    GAAGAATATG TGGATTTGAT GTTTGACTTT TGTATGCATA CGGGTATTCA GAAACAAATG
    GAAGCCTTTA GAGATGGGTT TAATAAAGTT TTTCCAATGG AGAAATTAAG TTCCTTCAGC
    CATGAAGAAG TCCAAATGAT TCTTTGTGGA AACCAGTCAC CATCCTGGGC AGCAGAGGAT
    ATTATCAATT ACACTGAACC TAAGCTGGGT TATACACGTG ACAGCCCTGG TTTCCTGAGG
    TTTGTGAGGG TTTTATGTGG CATGTCTTCT GATGAAAGGA AAGCATTCTT GCAGTTTACC
    ACTGGTTGTT CAACTCTACC CCCAGGTGGA CTGGCTAACC TGCATCCCAG GCTCACGGTT
    GTACGCAAGG TTGATGCTAC TGATGCAAGC TATCCATCAG TCAATACATG TGTGCATTAC
    CTTAAGTTGC CTGAATATTC TTCCGAGGAG ATCATGAGAG AGCGCCTGCT AGCTGCTACA
    ATGGAGAAAG GCTTTCATCT CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu66519
    Clone ID Related Accession (Same CDS sequence) XM_011536619.2 , XM_024449526.1
    Accession Version XM_024449526.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGGAAGT TAGGCCCTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TCAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAA
    GAAGCAGCTT CACAACGACC TCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAATCTT TAAGTAACAC TTTAACCACC
    AGCCTCACAT CAACTTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTATTGGCTG AGCTCGATGA TGATGAGGAC
    TTACCTGAGC CAGATGAAGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGA TTCTGAGACG CCCATCCCTG CAACGTCGAG CTGGCTCCCG CTCTGATGTA
    ACGCATCATG CTGTTACCTC GCAGCTACCA CAGGTACCTG CTGGAGCAGG GAGCCGACCT
    ATTGGGGAGC AGGAAGAAGA AGAGTACGAA ACTAAAGGAG GACGCCGGAG AACATGGGAT
    GATGATTATG TGCTAAAGAG ACAGTTTTCT GCATTGGTTC CTGCTTTTGA TCCTAGACCT
    GGTCGTACTA ATGTCCAGCA GACAACTGAT CTAGAAATAC CACCCCCAGG GACCCCTCAT
    TCAGAGCTCT TGGAAGAAGT CGAATGTACT CCGTCACCTC GATTAGCTCT CACTTTGAAA
    GTAACAGGTC TTGGAACGAC TCGTGAAGTT GAATTACCAC TCACCAATTT CAGATCAACC
    ATCTTTTACT ATGTACAAAA ATTGCTTCAA TTGTCCTGTA ATGGCAATGT GAAATCAGAT
    AAACTTAGGC GTATTTGGGA GCCCACATAC ACAATCATGT ACAGAGAAAT GAAGGATTCT
    GATAAAGAAA AGGAAAATGG AAAAATGGGT TGCTGGTCTA TAGAGCATGT GGAGCAGTAC
    CTTGGCACTG ATGAATTACC AAAGAATGAC TTGATAACCT ACCTGCAGAA GAATGCAGAC
    GCTGCTTTCC TGCGCCACTG GAAATTAACT GGCACTAATA AAAGTATTAG GAAAAACAGA
    AATTGTTCTC AGCTCATAGC TGCATATAAG GATTTTTGTG AGCATGGAAC AAAGTCTGGG
    TTAAACCAGG GGGCCATTTC TACTCTTCAA AGTAGTGATA TTCTTAATTT AACAAAAGAA
    CAACCTCAGG CCAAAGCAGG CAATGGACAG AACTCTTGTG GAGTAGAAGA TGTCCTTCAG
    CTTCTGCGTA TTCTATATAT AGTTGCAAGT GACCCTTATT CAAGAATATC CCAGGAAGAT
    GGTGATGAAC AGCCTCAGTT TACTTTTCCA CCAGATGAAT TCACTAGCAA AAAAATTACA
    ACAAAAATAT TACAGCAGAT TGAGGAACCA TTGGCACTGG CAAGTGGGGC TCTGCCAGAC
    TGGTGTGAAC AATTAACCAG CAAATGTCCT TTTCTAATAC CATTTGAAAC TAGACAGCTT
    TATTTCACAT GTACAGCATT TGGCGCCTCA AGAGCAATAG TATGGTTACA GAACCGACGT
    GAAGCCACTG TGGAGCGAAC GAGAACCACA AGCAGTGTTA GGCGAGATGA CCCTGGAGAG
    TTTCGAGTTG GTCGTCTCAA GCATGAAAGA GTAAAAGTTC CACGTGGCGA GTCACTGATG
    GAATGGGCTG AGAATGTCAT GCAAATACAT GCAGATCGGA AATCAGTTCT TGAGGTTGAA
    TTTTTAGGAG AAGAAGGAAC TGGCTTGGGA CCCACATTAG AGTTTTATGC TCTGGTGGCA
    GCAGAATTCC AGAGAACTGA CTTGGGAGCT TGGCTTTGTG ATGATAATTT TCCAGATGAT
    GAATCTCGTC ACGTTGATCT TGGAGGTGGA TTGAAACCTC CTGGATATTA TGTGCAGAGG
    TCATGTGGAC TGTTCACAGC ACCATTTCCA CAGGATAGTG ATGAGCTTGA AAGGATCACG
    AAACTGTTTC ATTTCCTTGG AATTTTCTTG GCCAAATGCA TTCAAGACAA TAGACTTGTG
    GACTTACCTA TTTCTAAACC TTTTTTTAAA CTTATGTGTA TGGGTGACAT TAAAAGCAAT
    ATGAGTAAAC TGATTTATGA GTCACGAGGT GATAGAGACT TACACTGTAC TGAAAGTCAG
    TCTGAAGCTT CTACAGAAGA AGGTCATGAT TCACTCTCGG TAGGAAGCTT TGAAGAGGAT
    TCAAAATCAG AATTTATTCT TGATCCCCCT AAACCAAAAC CCCCAGCTTG GTTTAATGGA
    ATTTTGACTT GGGAAGACTT TGAATTAGTA AACCCACACA GAGCCAGATT TTTAAAAGAA
    ATTAAAGACC TTGCTATCAA GAGGCGCCAA ATTTTAAGCA ACAAAGGTCT TTCTGAAGAT
    GAGAAGAACA CAAAATTACA GGAACTAGTG CTGAAGAATC CATCAGGTTC TGGGCCTCCA
    CTTAGCATAG AGGATTTAGG TTTAAATTTC CAGTTTTGCC CTTCCTCAAG AATATATGGT
    TTTACAGCTG TGGATCTCAA GCCAAGTGGT GAAGATGAGA TGATAACAAT GGATAATGCA
    GAAGAATATG TGGATTTGAT GTTTGACTTT TGTATGCATA CGGGTATTCA GAAACAAATG
    GAAGCCTTTA GAGATGGGTT TAATAAAGTT TTTCCAATGG AGAAATTAAG TTCCTTCAGC
    CATGAAGAAG TCCAAATGAT TCTTTGTGGA AACCAGTCAC CATCCTGGGC AGCAGAGGAT
    ATTATCAATT ACACTGAACC TAAGCTGGGT TATACACGTG ACAGCCCTGG TTTCCTGAGG
    TTTGTGAGGG TTTTATGTGG CATGTCTTCT GATGAAAGGA AAGCATTCTT GCAGTTTACC
    ACTGGTTGTT CAACTCTACC CCCAGGTGGA CTGGCTAACC TGCATCCCAG GCTCACGGTT
    GTACGCAAGG TTGATGCTAC TGATGCAAGC TATCCATCAG TCAATACATG TGTGCATTAC
    CTTAAGTTGC CTGAATATTC TTCCGAGGAG ATCATGAGAG AGCGCCTGCT AGCTGCTACA
    ATGGAGAAAG GCTTTCATCT CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024305294.1
    CDS530..8356
    Translation

    Target ORF information:

    RefSeq Version XM_024449526.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024449526.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGGAAGT TAGGCCCTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TCAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAA
    GAAGCAGCTT CACAACGACC TCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAATCTT TAAGTAACAC TTTAACCACC
    AGCCTCACAT CAACTTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTATTGGCTG AGCTCGATGA TGATGAGGAC
    TTACCTGAGC CAGATGAAGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGA TTCTGAGACG CCCATCCCTG CAACGTCGAG CTGGCTCCCG CTCTGATGTA
    ACGCATCATG CTGTTACCTC GCAGCTACCA CAGGTACCTG CTGGAGCAGG GAGCCGACCT
    ATTGGGGAGC AGGAAGAAGA AGAGTACGAA ACTAAAGGAG GACGCCGGAG AACATGGGAT
    GATGATTATG TGCTAAAGAG ACAGTTTTCT GCATTGGTTC CTGCTTTTGA TCCTAGACCT
    GGTCGTACTA ATGTCCAGCA GACAACTGAT CTAGAAATAC CACCCCCAGG GACCCCTCAT
    TCAGAGCTCT TGGAAGAAGT CGAATGTACT CCGTCACCTC GATTAGCTCT CACTTTGAAA
    GTAACAGGTC TTGGAACGAC TCGTGAAGTT GAATTACCAC TCACCAATTT CAGATCAACC
    ATCTTTTACT ATGTACAAAA ATTGCTTCAA TTGTCCTGTA ATGGCAATGT GAAATCAGAT
    AAACTTAGGC GTATTTGGGA GCCCACATAC ACAATCATGT ACAGAGAAAT GAAGGATTCT
    GATAAAGAAA AGGAAAATGG AAAAATGGGT TGCTGGTCTA TAGAGCATGT GGAGCAGTAC
    CTTGGCACTG ATGAATTACC AAAGAATGAC TTGATAACCT ACCTGCAGAA GAATGCAGAC
    GCTGCTTTCC TGCGCCACTG GAAATTAACT GGCACTAATA AAAGTATTAG GAAAAACAGA
    AATTGTTCTC AGCTCATAGC TGCATATAAG GATTTTTGTG AGCATGGAAC AAAGTCTGGG
    TTAAACCAGG GGGCCATTTC TACTCTTCAA AGTAGTGATA TTCTTAATTT AACAAAAGAA
    CAACCTCAGG CCAAAGCAGG CAATGGACAG AACTCTTGTG GAGTAGAAGA TGTCCTTCAG
    CTTCTGCGTA TTCTATATAT AGTTGCAAGT GACCCTTATT CAAGAATATC CCAGGAAGAT
    GGTGATGAAC AGCCTCAGTT TACTTTTCCA CCAGATGAAT TCACTAGCAA AAAAATTACA
    ACAAAAATAT TACAGCAGAT TGAGGAACCA TTGGCACTGG CAAGTGGGGC TCTGCCAGAC
    TGGTGTGAAC AATTAACCAG CAAATGTCCT TTTCTAATAC CATTTGAAAC TAGACAGCTT
    TATTTCACAT GTACAGCATT TGGCGCCTCA AGAGCAATAG TATGGTTACA GAACCGACGT
    GAAGCCACTG TGGAGCGAAC GAGAACCACA AGCAGTGTTA GGCGAGATGA CCCTGGAGAG
    TTTCGAGTTG GTCGTCTCAA GCATGAAAGA GTAAAAGTTC CACGTGGCGA GTCACTGATG
    GAATGGGCTG AGAATGTCAT GCAAATACAT GCAGATCGGA AATCAGTTCT TGAGGTTGAA
    TTTTTAGGAG AAGAAGGAAC TGGCTTGGGA CCCACATTAG AGTTTTATGC TCTGGTGGCA
    GCAGAATTCC AGAGAACTGA CTTGGGAGCT TGGCTTTGTG ATGATAATTT TCCAGATGAT
    GAATCTCGTC ACGTTGATCT TGGAGGTGGA TTGAAACCTC CTGGATATTA TGTGCAGAGG
    TCATGTGGAC TGTTCACAGC ACCATTTCCA CAGGATAGTG ATGAGCTTGA AAGGATCACG
    AAACTGTTTC ATTTCCTTGG AATTTTCTTG GCCAAATGCA TTCAAGACAA TAGACTTGTG
    GACTTACCTA TTTCTAAACC TTTTTTTAAA CTTATGTGTA TGGGTGACAT TAAAAGCAAT
    ATGAGTAAAC TGATTTATGA GTCACGAGGT GATAGAGACT TACACTGTAC TGAAAGTCAG
    TCTGAAGCTT CTACAGAAGA AGGTCATGAT TCACTCTCGG TAGGAAGCTT TGAAGAGGAT
    TCAAAATCAG AATTTATTCT TGATCCCCCT AAACCAAAAC CCCCAGCTTG GTTTAATGGA
    ATTTTGACTT GGGAAGACTT TGAATTAGTA AACCCACACA GAGCCAGATT TTTAAAAGAA
    ATTAAAGACC TTGCTATCAA GAGGCGCCAA ATTTTAAGCA ACAAAGGTCT TTCTGAAGAT
    GAGAAGAACA CAAAATTACA GGAACTAGTG CTGAAGAATC CATCAGGTTC TGGGCCTCCA
    CTTAGCATAG AGGATTTAGG TTTAAATTTC CAGTTTTGCC CTTCCTCAAG AATATATGGT
    TTTACAGCTG TGGATCTCAA GCCAAGTGGT GAAGATGAGA TGATAACAAT GGATAATGCA
    GAAGAATATG TGGATTTGAT GTTTGACTTT TGTATGCATA CGGGTATTCA GAAACAAATG
    GAAGCCTTTA GAGATGGGTT TAATAAAGTT TTTCCAATGG AGAAATTAAG TTCCTTCAGC
    CATGAAGAAG TCCAAATGAT TCTTTGTGGA AACCAGTCAC CATCCTGGGC AGCAGAGGAT
    ATTATCAATT ACACTGAACC TAAGCTGGGT TATACACGTG ACAGCCCTGG TTTCCTGAGG
    TTTGTGAGGG TTTTATGTGG CATGTCTTCT GATGAAAGGA AAGCATTCTT GCAGTTTACC
    ACTGGTTGTT CAACTCTACC CCCAGGTGGA CTGGCTAACC TGCATCCCAG GCTCACGGTT
    GTACGCAAGG TTGATGCTAC TGATGCAAGC TATCCATCAG TCAATACATG TGTGCATTAC
    CTTAAGTTGC CTGAATATTC TTCCGAGGAG ATCATGAGAG AGCGCCTGCT AGCTGCTACA
    ATGGAGAAAG GCTTTCATCT CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu66520
    Clone ID Related Accession (Same CDS sequence) XM_017021150.1 , XM_024449527.1
    Accession Version XM_017021150.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7650bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
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    3421
    3481
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    3601
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    3721
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    4141
    4201
    4261
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    4381
    4441
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    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGGAAGT TAGGCCCTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TCAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAA
    GAAGCAGCTT CACAACGACC TCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAATCTT TAAGTAACAC TTTAACCACC
    AGCCTCACAT CAACTTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTATTGGCTG AGCTCGATGA TGATGAGGAC
    TTACCTGAGC CAGATGAAGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGCCCA TCCCTGCAAC GTCGAGCTGG CTCCCGCTCT
    GATGTAACGC ATCATGCTGT TACCTCGCAG CTACCACAGG TACCTGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAAGAAGAG TACGAAACTA AAGGAGGACG CCGGAGAACA
    TGGGATGATG ATTATGTGCT AAAGAGACAG TTTTCTGCAT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GTACTAATGT CCAGCAGACA ACTGATCTAG AAATACCACC CCCAGGGACC
    CCTCATTCAG AGCTCTTGGA AGAAGTCGAA TGTACTCCGT CACCTCGATT AGCTCTCACT
    TTGAAAGTAA CAGGTCTTGG AACGACTCGT GAAGTTGAAT TACCACTCAC CAATTTCAGA
    TCAACCATCT TTTACTATGT ACAAAAATTG CTTCAATTGT CCTGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCC ACATACACAA TCATGTACAG AGAAATGAAG
    GATTCTGATA AAGAAAAGGA AAATGGAAAA ATGGATTTTT GTGAGCATGG AACAAAGTCT
    GGGTTAAACC AGGGGGCCAT TTCTACTCTT CAAAGTAGTG ATATTCTTAA TTTAACAAAA
    GAACAACCTC AGGCCAAAGC AGGCAATGGA CAGAACTCTT GTGGAGTAGA AGATGTCCTT
    CAGCTTCTGC GTATTCTATA TATAGTTGCA AGTGACCCTT ATTCAAGAAT ATCCCAGGAA
    GATGGTGATG AACAGCCTCA GTTTACTTTT CCACCAGATG AATTCACTAG CAAAAAAATT
    ACAACAAAAA TATTACAGCA GATTGAGGAA CCATTGGCAC TGGCAAGTGG GGCTCTGCCA
    GACTGGTGTG AACAATTAAC CAGCAAATGT CCTTTTCTAA TACCATTTGA AACTAGACAG
    CTTTATTTCA CATGTACAGC ATTTGGCGCC TCAAGAGCAA TAGTATGGTT ACAGAACCGA
    CGTGAAGCCA CTGTGGAGCG AACGAGAACC ACAAGCAGTG TTAGGCGAGA TGACCCTGGA
    GAGTTTCGAG TTGGTCGTCT CAAGCATGAA AGAGTAAAAG TTCCACGTGG CGAGTCACTG
    ATGGAATGGG CTGAGAATGT CATGCAAATA CATGCAGATC GGAAATCAGT TCTTGAGGTT
    GAATTTTTAG GAGAAGAAGG AACTGGCTTG GGACCCACAT TAGAGTTTTA TGCTCTGGTG
    GCAGCAGAAT TCCAGAGAAC TGACTTGGGA GCTTGGCTTT GTGATGATAA TTTTCCAGAT
    GATGAATCTC GTCACGTTGA TCTTGGAGGT GGATTGAAAC CTCCTGGATA TTATGTGCAG
    AGGTCATGTG GACTGTTCAC AGCACCATTT CCACAGGATA GTGATGAGCT TGAAAGGATC
    ACGAAACTGT TTCATTTCCT TGGAATTTTC TTGGCCAAAT GCATTCAAGA CAATAGACTT
    GTGGACTTAC CTATTTCTAA ACCTTTTTTT AAACTTATGT GTATGGGTGA CATTAAAAGC
    AATATGAGTA AACTGATTTA TGAGTCACGA GGTGATAGAG ACTTACACTG TACTGAAAGT
    CAGTCTGAAG CTTCTACAGA AGAAGGTCAT GATTCACTCT CGGTAGGAAG CTTTGAAGAG
    GATTCAAAAT CAGAATTTAT TCTTGATCCC CCTAAACCAA AACCCCCAGC TTGGTTTAAT
    GGAATTTTGA CTTGGGAAGA CTTTGAATTA GTAAACCCAC ACAGAGCCAG ATTTTTAAAA
    GAAATTAAAG ACCTTGCTAT CAAGAGGCGC CAAATTTTAA GCAACAAAGG TCTTTCTGAA
    GATGAGAAGA ACACAAAATT ACAGGAACTA GTGCTGAAGA ATCCATCAGG TTCTGGGCCT
    CCACTTAGCA TAGAGGATTT AGGTTTAAAT TTCCAGTTTT GCCCTTCCTC AAGAATATAT
    GGTTTTACAG CTGTGGATCT CAAGCCAAGT GGTGAAGATG AGATGATAAC AATGGATAAT
    GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTTTGTATGC ATACGGGTAT TCAGAAACAA
    ATGGAAGCCT TTAGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC
    AGCCATGAAG AAGTCCAAAT GATTCTTTGT GGAAACCAGT CACCATCCTG GGCAGCAGAG
    GATATTATCA ATTACACTGA ACCTAAGCTG GGTTATACAC GTGACAGCCC TGGTTTCCTG
    AGGTTTGTGA GGGTTTTATG TGGCATGTCT TCTGATGAAA GGAAAGCATT CTTGCAGTTT
    ACCACTGGTT GTTCAACTCT ACCCCCAGGT GGACTGGCTA ACCTGCATCC CAGGCTCACG
    GTTGTACGCA AGGTTGATGC TACTGATGCA AGCTATCCAT CAGTCAATAC ATGTGTGCAT
    TACCTTAAGT TGCCTGAATA TTCTTCCGAG GAGATCATGA GAGAGCGCCT GCTAGCTGCT
    ACAATGGAGA AAGGCTTTCA TCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876639.1
    CDS22..7671
    Translation

    Target ORF information:

    RefSeq Version XM_017021150.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017021150.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGGAAGT TAGGCCCTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TCAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAA
    GAAGCAGCTT CACAACGACC TCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAATCTT TAAGTAACAC TTTAACCACC
    AGCCTCACAT CAACTTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTATTGGCTG AGCTCGATGA TGATGAGGAC
    TTACCTGAGC CAGATGAAGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGCCCA TCCCTGCAAC GTCGAGCTGG CTCCCGCTCT
    GATGTAACGC ATCATGCTGT TACCTCGCAG CTACCACAGG TACCTGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAAGAAGAG TACGAAACTA AAGGAGGACG CCGGAGAACA
    TGGGATGATG ATTATGTGCT AAAGAGACAG TTTTCTGCAT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GTACTAATGT CCAGCAGACA ACTGATCTAG AAATACCACC CCCAGGGACC
    CCTCATTCAG AGCTCTTGGA AGAAGTCGAA TGTACTCCGT CACCTCGATT AGCTCTCACT
    TTGAAAGTAA CAGGTCTTGG AACGACTCGT GAAGTTGAAT TACCACTCAC CAATTTCAGA
    TCAACCATCT TTTACTATGT ACAAAAATTG CTTCAATTGT CCTGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCC ACATACACAA TCATGTACAG AGAAATGAAG
    GATTCTGATA AAGAAAAGGA AAATGGAAAA ATGGATTTTT GTGAGCATGG AACAAAGTCT
    GGGTTAAACC AGGGGGCCAT TTCTACTCTT CAAAGTAGTG ATATTCTTAA TTTAACAAAA
    GAACAACCTC AGGCCAAAGC AGGCAATGGA CAGAACTCTT GTGGAGTAGA AGATGTCCTT
    CAGCTTCTGC GTATTCTATA TATAGTTGCA AGTGACCCTT ATTCAAGAAT ATCCCAGGAA
    GATGGTGATG AACAGCCTCA GTTTACTTTT CCACCAGATG AATTCACTAG CAAAAAAATT
    ACAACAAAAA TATTACAGCA GATTGAGGAA CCATTGGCAC TGGCAAGTGG GGCTCTGCCA
    GACTGGTGTG AACAATTAAC CAGCAAATGT CCTTTTCTAA TACCATTTGA AACTAGACAG
    CTTTATTTCA CATGTACAGC ATTTGGCGCC TCAAGAGCAA TAGTATGGTT ACAGAACCGA
    CGTGAAGCCA CTGTGGAGCG AACGAGAACC ACAAGCAGTG TTAGGCGAGA TGACCCTGGA
    GAGTTTCGAG TTGGTCGTCT CAAGCATGAA AGAGTAAAAG TTCCACGTGG CGAGTCACTG
    ATGGAATGGG CTGAGAATGT CATGCAAATA CATGCAGATC GGAAATCAGT TCTTGAGGTT
    GAATTTTTAG GAGAAGAAGG AACTGGCTTG GGACCCACAT TAGAGTTTTA TGCTCTGGTG
    GCAGCAGAAT TCCAGAGAAC TGACTTGGGA GCTTGGCTTT GTGATGATAA TTTTCCAGAT
    GATGAATCTC GTCACGTTGA TCTTGGAGGT GGATTGAAAC CTCCTGGATA TTATGTGCAG
    AGGTCATGTG GACTGTTCAC AGCACCATTT CCACAGGATA GTGATGAGCT TGAAAGGATC
    ACGAAACTGT TTCATTTCCT TGGAATTTTC TTGGCCAAAT GCATTCAAGA CAATAGACTT
    GTGGACTTAC CTATTTCTAA ACCTTTTTTT AAACTTATGT GTATGGGTGA CATTAAAAGC
    AATATGAGTA AACTGATTTA TGAGTCACGA GGTGATAGAG ACTTACACTG TACTGAAAGT
    CAGTCTGAAG CTTCTACAGA AGAAGGTCAT GATTCACTCT CGGTAGGAAG CTTTGAAGAG
    GATTCAAAAT CAGAATTTAT TCTTGATCCC CCTAAACCAA AACCCCCAGC TTGGTTTAAT
    GGAATTTTGA CTTGGGAAGA CTTTGAATTA GTAAACCCAC ACAGAGCCAG ATTTTTAAAA
    GAAATTAAAG ACCTTGCTAT CAAGAGGCGC CAAATTTTAA GCAACAAAGG TCTTTCTGAA
    GATGAGAAGA ACACAAAATT ACAGGAACTA GTGCTGAAGA ATCCATCAGG TTCTGGGCCT
    CCACTTAGCA TAGAGGATTT AGGTTTAAAT TTCCAGTTTT GCCCTTCCTC AAGAATATAT
    GGTTTTACAG CTGTGGATCT CAAGCCAAGT GGTGAAGATG AGATGATAAC AATGGATAAT
    GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTTTGTATGC ATACGGGTAT TCAGAAACAA
    ATGGAAGCCT TTAGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC
    AGCCATGAAG AAGTCCAAAT GATTCTTTGT GGAAACCAGT CACCATCCTG GGCAGCAGAG
    GATATTATCA ATTACACTGA ACCTAAGCTG GGTTATACAC GTGACAGCCC TGGTTTCCTG
    AGGTTTGTGA GGGTTTTATG TGGCATGTCT TCTGATGAAA GGAAAGCATT CTTGCAGTTT
    ACCACTGGTT GTTCAACTCT ACCCCCAGGT GGACTGGCTA ACCTGCATCC CAGGCTCACG
    GTTGTACGCA AGGTTGATGC TACTGATGCA AGCTATCCAT CAGTCAATAC ATGTGTGCAT
    TACCTTAAGT TGCCTGAATA TTCTTCCGAG GAGATCATGA GAGAGCGCCT GCTAGCTGCT
    ACAATGGAGA AAGGCTTTCA TCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu66520
    Clone ID Related Accession (Same CDS sequence) XM_017021150.1 , XM_024449527.1
    Accession Version XM_024449527.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7650bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGGAAGT TAGGCCCTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TCAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAA
    GAAGCAGCTT CACAACGACC TCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAATCTT TAAGTAACAC TTTAACCACC
    AGCCTCACAT CAACTTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTATTGGCTG AGCTCGATGA TGATGAGGAC
    TTACCTGAGC CAGATGAAGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGCCCA TCCCTGCAAC GTCGAGCTGG CTCCCGCTCT
    GATGTAACGC ATCATGCTGT TACCTCGCAG CTACCACAGG TACCTGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAAGAAGAG TACGAAACTA AAGGAGGACG CCGGAGAACA
    TGGGATGATG ATTATGTGCT AAAGAGACAG TTTTCTGCAT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GTACTAATGT CCAGCAGACA ACTGATCTAG AAATACCACC CCCAGGGACC
    CCTCATTCAG AGCTCTTGGA AGAAGTCGAA TGTACTCCGT CACCTCGATT AGCTCTCACT
    TTGAAAGTAA CAGGTCTTGG AACGACTCGT GAAGTTGAAT TACCACTCAC CAATTTCAGA
    TCAACCATCT TTTACTATGT ACAAAAATTG CTTCAATTGT CCTGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCC ACATACACAA TCATGTACAG AGAAATGAAG
    GATTCTGATA AAGAAAAGGA AAATGGAAAA ATGGATTTTT GTGAGCATGG AACAAAGTCT
    GGGTTAAACC AGGGGGCCAT TTCTACTCTT CAAAGTAGTG ATATTCTTAA TTTAACAAAA
    GAACAACCTC AGGCCAAAGC AGGCAATGGA CAGAACTCTT GTGGAGTAGA AGATGTCCTT
    CAGCTTCTGC GTATTCTATA TATAGTTGCA AGTGACCCTT ATTCAAGAAT ATCCCAGGAA
    GATGGTGATG AACAGCCTCA GTTTACTTTT CCACCAGATG AATTCACTAG CAAAAAAATT
    ACAACAAAAA TATTACAGCA GATTGAGGAA CCATTGGCAC TGGCAAGTGG GGCTCTGCCA
    GACTGGTGTG AACAATTAAC CAGCAAATGT CCTTTTCTAA TACCATTTGA AACTAGACAG
    CTTTATTTCA CATGTACAGC ATTTGGCGCC TCAAGAGCAA TAGTATGGTT ACAGAACCGA
    CGTGAAGCCA CTGTGGAGCG AACGAGAACC ACAAGCAGTG TTAGGCGAGA TGACCCTGGA
    GAGTTTCGAG TTGGTCGTCT CAAGCATGAA AGAGTAAAAG TTCCACGTGG CGAGTCACTG
    ATGGAATGGG CTGAGAATGT CATGCAAATA CATGCAGATC GGAAATCAGT TCTTGAGGTT
    GAATTTTTAG GAGAAGAAGG AACTGGCTTG GGACCCACAT TAGAGTTTTA TGCTCTGGTG
    GCAGCAGAAT TCCAGAGAAC TGACTTGGGA GCTTGGCTTT GTGATGATAA TTTTCCAGAT
    GATGAATCTC GTCACGTTGA TCTTGGAGGT GGATTGAAAC CTCCTGGATA TTATGTGCAG
    AGGTCATGTG GACTGTTCAC AGCACCATTT CCACAGGATA GTGATGAGCT TGAAAGGATC
    ACGAAACTGT TTCATTTCCT TGGAATTTTC TTGGCCAAAT GCATTCAAGA CAATAGACTT
    GTGGACTTAC CTATTTCTAA ACCTTTTTTT AAACTTATGT GTATGGGTGA CATTAAAAGC
    AATATGAGTA AACTGATTTA TGAGTCACGA GGTGATAGAG ACTTACACTG TACTGAAAGT
    CAGTCTGAAG CTTCTACAGA AGAAGGTCAT GATTCACTCT CGGTAGGAAG CTTTGAAGAG
    GATTCAAAAT CAGAATTTAT TCTTGATCCC CCTAAACCAA AACCCCCAGC TTGGTTTAAT
    GGAATTTTGA CTTGGGAAGA CTTTGAATTA GTAAACCCAC ACAGAGCCAG ATTTTTAAAA
    GAAATTAAAG ACCTTGCTAT CAAGAGGCGC CAAATTTTAA GCAACAAAGG TCTTTCTGAA
    GATGAGAAGA ACACAAAATT ACAGGAACTA GTGCTGAAGA ATCCATCAGG TTCTGGGCCT
    CCACTTAGCA TAGAGGATTT AGGTTTAAAT TTCCAGTTTT GCCCTTCCTC AAGAATATAT
    GGTTTTACAG CTGTGGATCT CAAGCCAAGT GGTGAAGATG AGATGATAAC AATGGATAAT
    GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTTTGTATGC ATACGGGTAT TCAGAAACAA
    ATGGAAGCCT TTAGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC
    AGCCATGAAG AAGTCCAAAT GATTCTTTGT GGAAACCAGT CACCATCCTG GGCAGCAGAG
    GATATTATCA ATTACACTGA ACCTAAGCTG GGTTATACAC GTGACAGCCC TGGTTTCCTG
    AGGTTTGTGA GGGTTTTATG TGGCATGTCT TCTGATGAAA GGAAAGCATT CTTGCAGTTT
    ACCACTGGTT GTTCAACTCT ACCCCCAGGT GGACTGGCTA ACCTGCATCC CAGGCTCACG
    GTTGTACGCA AGGTTGATGC TACTGATGCA AGCTATCCAT CAGTCAATAC ATGTGTGCAT
    TACCTTAAGT TGCCTGAATA TTCTTCCGAG GAGATCATGA GAGAGCGCCT GCTAGCTGCT
    ACAATGGAGA AAGGCTTTCA TCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024305295.1
    CDS490..8139
    Translation

    Target ORF information:

    RefSeq Version XM_024449527.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024449527.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGGAAGT TAGGCCCTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TCAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAA
    GAAGCAGCTT CACAACGACC TCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAATCTT TAAGTAACAC TTTAACCACC
    AGCCTCACAT CAACTTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTATTGGCTG AGCTCGATGA TGATGAGGAC
    TTACCTGAGC CAGATGAAGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGCCCA TCCCTGCAAC GTCGAGCTGG CTCCCGCTCT
    GATGTAACGC ATCATGCTGT TACCTCGCAG CTACCACAGG TACCTGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAAGAAGAG TACGAAACTA AAGGAGGACG CCGGAGAACA
    TGGGATGATG ATTATGTGCT AAAGAGACAG TTTTCTGCAT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GTACTAATGT CCAGCAGACA ACTGATCTAG AAATACCACC CCCAGGGACC
    CCTCATTCAG AGCTCTTGGA AGAAGTCGAA TGTACTCCGT CACCTCGATT AGCTCTCACT
    TTGAAAGTAA CAGGTCTTGG AACGACTCGT GAAGTTGAAT TACCACTCAC CAATTTCAGA
    TCAACCATCT TTTACTATGT ACAAAAATTG CTTCAATTGT CCTGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCC ACATACACAA TCATGTACAG AGAAATGAAG
    GATTCTGATA AAGAAAAGGA AAATGGAAAA ATGGATTTTT GTGAGCATGG AACAAAGTCT
    GGGTTAAACC AGGGGGCCAT TTCTACTCTT CAAAGTAGTG ATATTCTTAA TTTAACAAAA
    GAACAACCTC AGGCCAAAGC AGGCAATGGA CAGAACTCTT GTGGAGTAGA AGATGTCCTT
    CAGCTTCTGC GTATTCTATA TATAGTTGCA AGTGACCCTT ATTCAAGAAT ATCCCAGGAA
    GATGGTGATG AACAGCCTCA GTTTACTTTT CCACCAGATG AATTCACTAG CAAAAAAATT
    ACAACAAAAA TATTACAGCA GATTGAGGAA CCATTGGCAC TGGCAAGTGG GGCTCTGCCA
    GACTGGTGTG AACAATTAAC CAGCAAATGT CCTTTTCTAA TACCATTTGA AACTAGACAG
    CTTTATTTCA CATGTACAGC ATTTGGCGCC TCAAGAGCAA TAGTATGGTT ACAGAACCGA
    CGTGAAGCCA CTGTGGAGCG AACGAGAACC ACAAGCAGTG TTAGGCGAGA TGACCCTGGA
    GAGTTTCGAG TTGGTCGTCT CAAGCATGAA AGAGTAAAAG TTCCACGTGG CGAGTCACTG
    ATGGAATGGG CTGAGAATGT CATGCAAATA CATGCAGATC GGAAATCAGT TCTTGAGGTT
    GAATTTTTAG GAGAAGAAGG AACTGGCTTG GGACCCACAT TAGAGTTTTA TGCTCTGGTG
    GCAGCAGAAT TCCAGAGAAC TGACTTGGGA GCTTGGCTTT GTGATGATAA TTTTCCAGAT
    GATGAATCTC GTCACGTTGA TCTTGGAGGT GGATTGAAAC CTCCTGGATA TTATGTGCAG
    AGGTCATGTG GACTGTTCAC AGCACCATTT CCACAGGATA GTGATGAGCT TGAAAGGATC
    ACGAAACTGT TTCATTTCCT TGGAATTTTC TTGGCCAAAT GCATTCAAGA CAATAGACTT
    GTGGACTTAC CTATTTCTAA ACCTTTTTTT AAACTTATGT GTATGGGTGA CATTAAAAGC
    AATATGAGTA AACTGATTTA TGAGTCACGA GGTGATAGAG ACTTACACTG TACTGAAAGT
    CAGTCTGAAG CTTCTACAGA AGAAGGTCAT GATTCACTCT CGGTAGGAAG CTTTGAAGAG
    GATTCAAAAT CAGAATTTAT TCTTGATCCC CCTAAACCAA AACCCCCAGC TTGGTTTAAT
    GGAATTTTGA CTTGGGAAGA CTTTGAATTA GTAAACCCAC ACAGAGCCAG ATTTTTAAAA
    GAAATTAAAG ACCTTGCTAT CAAGAGGCGC CAAATTTTAA GCAACAAAGG TCTTTCTGAA
    GATGAGAAGA ACACAAAATT ACAGGAACTA GTGCTGAAGA ATCCATCAGG TTCTGGGCCT
    CCACTTAGCA TAGAGGATTT AGGTTTAAAT TTCCAGTTTT GCCCTTCCTC AAGAATATAT
    GGTTTTACAG CTGTGGATCT CAAGCCAAGT GGTGAAGATG AGATGATAAC AATGGATAAT
    GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTTTGTATGC ATACGGGTAT TCAGAAACAA
    ATGGAAGCCT TTAGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC
    AGCCATGAAG AAGTCCAAAT GATTCTTTGT GGAAACCAGT CACCATCCTG GGCAGCAGAG
    GATATTATCA ATTACACTGA ACCTAAGCTG GGTTATACAC GTGACAGCCC TGGTTTCCTG
    AGGTTTGTGA GGGTTTTATG TGGCATGTCT TCTGATGAAA GGAAAGCATT CTTGCAGTTT
    ACCACTGGTT GTTCAACTCT ACCCCCAGGT GGACTGGCTA ACCTGCATCC CAGGCTCACG
    GTTGTACGCA AGGTTGATGC TACTGATGCA AGCTATCCAT CAGTCAATAC ATGTGTGCAT
    TACCTTAAGT TGCCTGAATA TTCTTCCGAG GAGATCATGA GAGAGCGCCT GCTAGCTGCT
    ACAATGGAGA AAGGCTTTCA TCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu66521
    Clone ID Related Accession (Same CDS sequence) XM_017021151.1 , XM_011536621.2 , XM_017021151.2
    Accession Version XM_017021151.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCTG GTACTACATC AACAGTGACT ATGTCAACAT CCAGTGTTAC TAGCAGCAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTTGGTCAAT CTTTAAGTAA CACTTTAACC
    ACCAGCCTCA CATCAACTTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTATTGG CTGAGCTCGA TGATGATGAG
    GACTTACCTG AGCCAGATGA AGAAGATGAT GAGAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGGTTATGAT TCTGAGACGC CCATCCCTGC AACGTCGAGC TGGCTCCCGC
    TCTGATGTAA CGCATCATGC TGTTACCTCG CAGCTACCAC AGGTACCTGC TGGAGCAGGG
    AGCCGACCTA TTGGGGAGCA GGAAGAAGAA GAGTACGAAA CTAAAGGAGG ACGCCGGAGA
    ACATGGGATG ATGATTATGT GCTAAAGAGA CAGTTTTCTG CATTGGTTCC TGCTTTTGAT
    CCTAGACCTG GTCGTACTAA TGTCCAGCAG ACAACTGATC TAGAAATACC ACCCCCAGGG
    ACCCCTCATT CAGAGCTCTT GGAAGAAGTC GAATGTACTC CGTCACCTCG ATTAGCTCTC
    ACTTTGAAAG TAACAGGTCT TGGAACGACT CGTGAAGTTG AATTACCACT CACCAATTTC
    AGATCAACCA TCTTTTACTA TGTACAAAAA TTGCTTCAAT TGTCCTGTAA TGGCAATGTG
    AAATCAGATA AACTTAGGCG TATTTGGGAG CCCACATACA CAATCATGTA CAGAGAAATG
    AAGGATTCTG ATAAAGAAAA GGAAAATGGA AAAATGGGTT GCTGGTCTAT AGAGCATGTG
    GAGCAGTACC TTGGCACTGA TGAATTACCA AAGAATGACT TGATAACCTA CCTGCAGAAG
    AATGCAGACG CTGCTTTCCT GCGCCACTGG AAATTAACTG GCACTAATAA AAGTATTAGG
    AAAAACAGAA ATTGTTCTCA GCTCATAGCT GCATATAAGG ATTTTTGTGA GCATGGAACA
    AAGTCTGGGT TAAACCAGGG GGCCATTTCT ACTCTTCAAA GTAGTGATAT TCTTAATTTA
    ACAAAAGAAC AACCTCAGGC CAAAGCAGGC AATGGACAGA ACTCTTGTGG AGTAGAAGAT
    GTCCTTCAGC TTCTGCGTAT TCTATATATA GTTGCAAGTG ACCCTTATTC AAGAATATCC
    CAGGAAGATG GTGATGAACA GCCTCAGTTT ACTTTTCCAC CAGATGAATT CACTAGCAAA
    AAAATTACAA CAAAAATATT ACAGCAGATT GAGGAACCAT TGGCACTGGC AAGTGGGGCT
    CTGCCAGACT GGTGTGAACA ATTAACCAGC AAATGTCCTT TTCTAATACC ATTTGAAACT
    AGACAGCTTT ATTTCACATG TACAGCATTT GGCGCCTCAA GAGCAATAGT ATGGTTACAG
    AACCGACGTG AAGCCACTGT GGAGCGAACG AGAACCACAA GCAGTGTTAG GCGAGATGAC
    CCTGGAGAGT TTCGAGTTGG TCGTCTCAAG CATGAAAGAG TAAAAGTTCC ACGTGGCGAG
    TCACTGATGG AATGGGCTGA GAATGTCATG CAAATACATG CAGATCGGAA ATCAGTTCTT
    GAGGTTGAAT TTTTAGGAGA AGAAGGAACT GGCTTGGGAC CCACATTAGA GTTTTATGCT
    CTGGTGGCAG CAGAATTCCA GAGAACTGAC TTGGGAGCTT GGCTTTGTGA TGATAATTTT
    CCAGATGATG AATCTCGTCA CGTTGATCTT GGAGGTGGAT TGAAACCTCC TGGATATTAT
    GTGCAGAGGT CATGTGGACT GTTCACAGCA CCATTTCCAC AGGATAGTGA TGAGCTTGAA
    AGGATCACGA AACTGTTTCA TTTCCTTGGA ATTTTCTTGG CCAAATGCAT TCAAGACAAT
    AGACTTGTGG ACTTACCTAT TTCTAAACCT TTTTTTAAAC TTATGTGTAT GGGTGACATT
    AAAAGCAATA TGAGTAAACT GATTTATGAG TCACGAGGTG ATAGAGACTT ACACTGTACT
    GAAAGTCAGT CTGAAGCTTC TACAGAAGAA GGTCATGATT CACTCTCGGT AGGAAGCTTT
    GAAGAGGATT CAAAATCAGA ATTTATTCTT GATCCCCCTA AACCAAAACC CCCAGCTTGG
    TTTAATGGAA TTTTGACTTG GGAAGACTTT GAATTAGTAA ACCCACACAG AGCCAGATTT
    TTAAAAGAAA TTAAAGACCT TGCTATCAAG AGGCGCCAAA TTTTAAGCAA CAAAGGTCTT
    TCTGAAGATG AGAAGAACAC AAAATTACAG GAACTAGTGC TGAAGAATCC ATCAGGTTCT
    GGGCCTCCAC TTAGCATAGA GGATTTAGGT TTAAATTTCC AGTTTTGCCC TTCCTCAAGA
    ATATATGGTT TTACAGCTGT GGATCTCAAG CCAAGTGGTG AAGATGAGAT GATAACAATG
    GATAATGCAG AAGAATATGT GGATTTGATG TTTGACTTTT GTATGCATAC GGGTATTCAG
    AAACAAATGG AAGCCTTTAG AGATGGGTTT AATAAAGTTT TTCCAATGGA GAAATTAAGT
    TCCTTCAGCC ATGAAGAAGT CCAAATGATT CTTTGTGGAA ACCAGTCACC ATCCTGGGCA
    GCAGAGGATA TTATCAATTA CACTGAACCT AAGCTGGGTT ATACACGTGA CAGCCCTGGT
    TTCCTGAGGT TTGTGAGGGT TTTATGTGGC ATGTCTTCTG ATGAAAGGAA AGCATTCTTG
    CAGTTTACCA CTGGTTGTTC AACTCTACCC CCAGGTGGAC TGGCTAACCT GCATCCCAGG
    CTCACGGTTG TACGCAAGGT TGATGCTACT GATGCAAGCT ATCCATCAGT CAATACATGT
    GTGCATTACC TTAAGTTGCC TGAATATTCT TCCGAGGAGA TCATGAGAGA GCGCCTGCTA
    GCTGCTACAA TGGAGAAAGG CTTTCATCTC AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876640.1
    CDS724..7719
    Translation

    Target ORF information:

    RefSeq Version XM_017021151.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017021151.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCTG GTACTACATC AACAGTGACT ATGTCAACAT CCAGTGTTAC TAGCAGCAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTTGGTCAAT CTTTAAGTAA CACTTTAACC
    ACCAGCCTCA CATCAACTTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTATTGG CTGAGCTCGA TGATGATGAG
    GACTTACCTG AGCCAGATGA AGAAGATGAT GAGAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGGTTATGAT TCTGAGACGC CCATCCCTGC AACGTCGAGC TGGCTCCCGC
    TCTGATGTAA CGCATCATGC TGTTACCTCG CAGCTACCAC AGGTACCTGC TGGAGCAGGG
    AGCCGACCTA TTGGGGAGCA GGAAGAAGAA GAGTACGAAA CTAAAGGAGG ACGCCGGAGA
    ACATGGGATG ATGATTATGT GCTAAAGAGA CAGTTTTCTG CATTGGTTCC TGCTTTTGAT
    CCTAGACCTG GTCGTACTAA TGTCCAGCAG ACAACTGATC TAGAAATACC ACCCCCAGGG
    ACCCCTCATT CAGAGCTCTT GGAAGAAGTC GAATGTACTC CGTCACCTCG ATTAGCTCTC
    ACTTTGAAAG TAACAGGTCT TGGAACGACT CGTGAAGTTG AATTACCACT CACCAATTTC
    AGATCAACCA TCTTTTACTA TGTACAAAAA TTGCTTCAAT TGTCCTGTAA TGGCAATGTG
    AAATCAGATA AACTTAGGCG TATTTGGGAG CCCACATACA CAATCATGTA CAGAGAAATG
    AAGGATTCTG ATAAAGAAAA GGAAAATGGA AAAATGGGTT GCTGGTCTAT AGAGCATGTG
    GAGCAGTACC TTGGCACTGA TGAATTACCA AAGAATGACT TGATAACCTA CCTGCAGAAG
    AATGCAGACG CTGCTTTCCT GCGCCACTGG AAATTAACTG GCACTAATAA AAGTATTAGG
    AAAAACAGAA ATTGTTCTCA GCTCATAGCT GCATATAAGG ATTTTTGTGA GCATGGAACA
    AAGTCTGGGT TAAACCAGGG GGCCATTTCT ACTCTTCAAA GTAGTGATAT TCTTAATTTA
    ACAAAAGAAC AACCTCAGGC CAAAGCAGGC AATGGACAGA ACTCTTGTGG AGTAGAAGAT
    GTCCTTCAGC TTCTGCGTAT TCTATATATA GTTGCAAGTG ACCCTTATTC AAGAATATCC
    CAGGAAGATG GTGATGAACA GCCTCAGTTT ACTTTTCCAC CAGATGAATT CACTAGCAAA
    AAAATTACAA CAAAAATATT ACAGCAGATT GAGGAACCAT TGGCACTGGC AAGTGGGGCT
    CTGCCAGACT GGTGTGAACA ATTAACCAGC AAATGTCCTT TTCTAATACC ATTTGAAACT
    AGACAGCTTT ATTTCACATG TACAGCATTT GGCGCCTCAA GAGCAATAGT ATGGTTACAG
    AACCGACGTG AAGCCACTGT GGAGCGAACG AGAACCACAA GCAGTGTTAG GCGAGATGAC
    CCTGGAGAGT TTCGAGTTGG TCGTCTCAAG CATGAAAGAG TAAAAGTTCC ACGTGGCGAG
    TCACTGATGG AATGGGCTGA GAATGTCATG CAAATACATG CAGATCGGAA ATCAGTTCTT
    GAGGTTGAAT TTTTAGGAGA AGAAGGAACT GGCTTGGGAC CCACATTAGA GTTTTATGCT
    CTGGTGGCAG CAGAATTCCA GAGAACTGAC TTGGGAGCTT GGCTTTGTGA TGATAATTTT
    CCAGATGATG AATCTCGTCA CGTTGATCTT GGAGGTGGAT TGAAACCTCC TGGATATTAT
    GTGCAGAGGT CATGTGGACT GTTCACAGCA CCATTTCCAC AGGATAGTGA TGAGCTTGAA
    AGGATCACGA AACTGTTTCA TTTCCTTGGA ATTTTCTTGG CCAAATGCAT TCAAGACAAT
    AGACTTGTGG ACTTACCTAT TTCTAAACCT TTTTTTAAAC TTATGTGTAT GGGTGACATT
    AAAAGCAATA TGAGTAAACT GATTTATGAG TCACGAGGTG ATAGAGACTT ACACTGTACT
    GAAAGTCAGT CTGAAGCTTC TACAGAAGAA GGTCATGATT CACTCTCGGT AGGAAGCTTT
    GAAGAGGATT CAAAATCAGA ATTTATTCTT GATCCCCCTA AACCAAAACC CCCAGCTTGG
    TTTAATGGAA TTTTGACTTG GGAAGACTTT GAATTAGTAA ACCCACACAG AGCCAGATTT
    TTAAAAGAAA TTAAAGACCT TGCTATCAAG AGGCGCCAAA TTTTAAGCAA CAAAGGTCTT
    TCTGAAGATG AGAAGAACAC AAAATTACAG GAACTAGTGC TGAAGAATCC ATCAGGTTCT
    GGGCCTCCAC TTAGCATAGA GGATTTAGGT TTAAATTTCC AGTTTTGCCC TTCCTCAAGA
    ATATATGGTT TTACAGCTGT GGATCTCAAG CCAAGTGGTG AAGATGAGAT GATAACAATG
    GATAATGCAG AAGAATATGT GGATTTGATG TTTGACTTTT GTATGCATAC GGGTATTCAG
    AAACAAATGG AAGCCTTTAG AGATGGGTTT AATAAAGTTT TTCCAATGGA GAAATTAAGT
    TCCTTCAGCC ATGAAGAAGT CCAAATGATT CTTTGTGGAA ACCAGTCACC ATCCTGGGCA
    GCAGAGGATA TTATCAATTA CACTGAACCT AAGCTGGGTT ATACACGTGA CAGCCCTGGT
    TTCCTGAGGT TTGTGAGGGT TTTATGTGGC ATGTCTTCTG ATGAAAGGAA AGCATTCTTG
    CAGTTTACCA CTGGTTGTTC AACTCTACCC CCAGGTGGAC TGGCTAACCT GCATCCCAGG
    CTCACGGTTG TACGCAAGGT TGATGCTACT GATGCAAGCT ATCCATCAGT CAATACATGT
    GTGCATTACC TTAAGTTGCC TGAATATTCT TCCGAGGAGA TCATGAGAGA GCGCCTGCTA
    GCTGCTACAA TGGAGAAAGG CTTTCATCTC AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu66521
    Clone ID Related Accession (Same CDS sequence) XM_017021151.1 , XM_011536621.2 , XM_017021151.2
    Accession Version XM_011536621.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011536621.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCTG GTACTACATC AACAGTGACT ATGTCAACAT CCAGTGTTAC TAGCAGCAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTTGGTCAAT CTTTAAGTAA CACTTTAACC
    ACCAGCCTCA CATCAACTTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTATTGG CTGAGCTCGA TGATGATGAG
    GACTTACCTG AGCCAGATGA AGAAGATGAT GAGAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGGTTATGAT TCTGAGACGC CCATCCCTGC AACGTCGAGC TGGCTCCCGC
    TCTGATGTAA CGCATCATGC TGTTACCTCG CAGCTACCAC AGGTACCTGC TGGAGCAGGG
    AGCCGACCTA TTGGGGAGCA GGAAGAAGAA GAGTACGAAA CTAAAGGAGG ACGCCGGAGA
    ACATGGGATG ATGATTATGT GCTAAAGAGA CAGTTTTCTG CATTGGTTCC TGCTTTTGAT
    CCTAGACCTG GTCGTACTAA TGTCCAGCAG ACAACTGATC TAGAAATACC ACCCCCAGGG
    ACCCCTCATT CAGAGCTCTT GGAAGAAGTC GAATGTACTC CGTCACCTCG ATTAGCTCTC
    ACTTTGAAAG TAACAGGTCT TGGAACGACT CGTGAAGTTG AATTACCACT CACCAATTTC
    AGATCAACCA TCTTTTACTA TGTACAAAAA TTGCTTCAAT TGTCCTGTAA TGGCAATGTG
    AAATCAGATA AACTTAGGCG TATTTGGGAG CCCACATACA CAATCATGTA CAGAGAAATG
    AAGGATTCTG ATAAAGAAAA GGAAAATGGA AAAATGGGTT GCTGGTCTAT AGAGCATGTG
    GAGCAGTACC TTGGCACTGA TGAATTACCA AAGAATGACT TGATAACCTA CCTGCAGAAG
    AATGCAGACG CTGCTTTCCT GCGCCACTGG AAATTAACTG GCACTAATAA AAGTATTAGG
    AAAAACAGAA ATTGTTCTCA GCTCATAGCT GCATATAAGG ATTTTTGTGA GCATGGAACA
    AAGTCTGGGT TAAACCAGGG GGCCATTTCT ACTCTTCAAA GTAGTGATAT TCTTAATTTA
    ACAAAAGAAC AACCTCAGGC CAAAGCAGGC AATGGACAGA ACTCTTGTGG AGTAGAAGAT
    GTCCTTCAGC TTCTGCGTAT TCTATATATA GTTGCAAGTG ACCCTTATTC AAGAATATCC
    CAGGAAGATG GTGATGAACA GCCTCAGTTT ACTTTTCCAC CAGATGAATT CACTAGCAAA
    AAAATTACAA CAAAAATATT ACAGCAGATT GAGGAACCAT TGGCACTGGC AAGTGGGGCT
    CTGCCAGACT GGTGTGAACA ATTAACCAGC AAATGTCCTT TTCTAATACC ATTTGAAACT
    AGACAGCTTT ATTTCACATG TACAGCATTT GGCGCCTCAA GAGCAATAGT ATGGTTACAG
    AACCGACGTG AAGCCACTGT GGAGCGAACG AGAACCACAA GCAGTGTTAG GCGAGATGAC
    CCTGGAGAGT TTCGAGTTGG TCGTCTCAAG CATGAAAGAG TAAAAGTTCC ACGTGGCGAG
    TCACTGATGG AATGGGCTGA GAATGTCATG CAAATACATG CAGATCGGAA ATCAGTTCTT
    GAGGTTGAAT TTTTAGGAGA AGAAGGAACT GGCTTGGGAC CCACATTAGA GTTTTATGCT
    CTGGTGGCAG CAGAATTCCA GAGAACTGAC TTGGGAGCTT GGCTTTGTGA TGATAATTTT
    CCAGATGATG AATCTCGTCA CGTTGATCTT GGAGGTGGAT TGAAACCTCC TGGATATTAT
    GTGCAGAGGT CATGTGGACT GTTCACAGCA CCATTTCCAC AGGATAGTGA TGAGCTTGAA
    AGGATCACGA AACTGTTTCA TTTCCTTGGA ATTTTCTTGG CCAAATGCAT TCAAGACAAT
    AGACTTGTGG ACTTACCTAT TTCTAAACCT TTTTTTAAAC TTATGTGTAT GGGTGACATT
    AAAAGCAATA TGAGTAAACT GATTTATGAG TCACGAGGTG ATAGAGACTT ACACTGTACT
    GAAAGTCAGT CTGAAGCTTC TACAGAAGAA GGTCATGATT CACTCTCGGT AGGAAGCTTT
    GAAGAGGATT CAAAATCAGA ATTTATTCTT GATCCCCCTA AACCAAAACC CCCAGCTTGG
    TTTAATGGAA TTTTGACTTG GGAAGACTTT GAATTAGTAA ACCCACACAG AGCCAGATTT
    TTAAAAGAAA TTAAAGACCT TGCTATCAAG AGGCGCCAAA TTTTAAGCAA CAAAGGTCTT
    TCTGAAGATG AGAAGAACAC AAAATTACAG GAACTAGTGC TGAAGAATCC ATCAGGTTCT
    GGGCCTCCAC TTAGCATAGA GGATTTAGGT TTAAATTTCC AGTTTTGCCC TTCCTCAAGA
    ATATATGGTT TTACAGCTGT GGATCTCAAG CCAAGTGGTG AAGATGAGAT GATAACAATG
    GATAATGCAG AAGAATATGT GGATTTGATG TTTGACTTTT GTATGCATAC GGGTATTCAG
    AAACAAATGG AAGCCTTTAG AGATGGGTTT AATAAAGTTT TTCCAATGGA GAAATTAAGT
    TCCTTCAGCC ATGAAGAAGT CCAAATGATT CTTTGTGGAA ACCAGTCACC ATCCTGGGCA
    GCAGAGGATA TTATCAATTA CACTGAACCT AAGCTGGGTT ATACACGTGA CAGCCCTGGT
    TTCCTGAGGT TTGTGAGGGT TTTATGTGGC ATGTCTTCTG ATGAAAGGAA AGCATTCTTG
    CAGTTTACCA CTGGTTGTTC AACTCTACCC CCAGGTGGAC TGGCTAACCT GCATCCCAGG
    CTCACGGTTG TACGCAAGGT TGATGCTACT GATGCAAGCT ATCCATCAGT CAATACATGT
    GTGCATTACC TTAAGTTGCC TGAATATTCT TCCGAGGAGA TCATGAGAGA GCGCCTGCTA
    GCTGCTACAA TGGAGAAAGG CTTTCATCTC AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011534923.1
    CDS128..7123
    Translation

    Target ORF information:

    RefSeq Version XM_011536621.2
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011536621.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCTG GTACTACATC AACAGTGACT ATGTCAACAT CCAGTGTTAC TAGCAGCAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTTGGTCAAT CTTTAAGTAA CACTTTAACC
    ACCAGCCTCA CATCAACTTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTATTGG CTGAGCTCGA TGATGATGAG
    GACTTACCTG AGCCAGATGA AGAAGATGAT GAGAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGGTTATGAT TCTGAGACGC CCATCCCTGC AACGTCGAGC TGGCTCCCGC
    TCTGATGTAA CGCATCATGC TGTTACCTCG CAGCTACCAC AGGTACCTGC TGGAGCAGGG
    AGCCGACCTA TTGGGGAGCA GGAAGAAGAA GAGTACGAAA CTAAAGGAGG ACGCCGGAGA
    ACATGGGATG ATGATTATGT GCTAAAGAGA CAGTTTTCTG CATTGGTTCC TGCTTTTGAT
    CCTAGACCTG GTCGTACTAA TGTCCAGCAG ACAACTGATC TAGAAATACC ACCCCCAGGG
    ACCCCTCATT CAGAGCTCTT GGAAGAAGTC GAATGTACTC CGTCACCTCG ATTAGCTCTC
    ACTTTGAAAG TAACAGGTCT TGGAACGACT CGTGAAGTTG AATTACCACT CACCAATTTC
    AGATCAACCA TCTTTTACTA TGTACAAAAA TTGCTTCAAT TGTCCTGTAA TGGCAATGTG
    AAATCAGATA AACTTAGGCG TATTTGGGAG CCCACATACA CAATCATGTA CAGAGAAATG
    AAGGATTCTG ATAAAGAAAA GGAAAATGGA AAAATGGGTT GCTGGTCTAT AGAGCATGTG
    GAGCAGTACC TTGGCACTGA TGAATTACCA AAGAATGACT TGATAACCTA CCTGCAGAAG
    AATGCAGACG CTGCTTTCCT GCGCCACTGG AAATTAACTG GCACTAATAA AAGTATTAGG
    AAAAACAGAA ATTGTTCTCA GCTCATAGCT GCATATAAGG ATTTTTGTGA GCATGGAACA
    AAGTCTGGGT TAAACCAGGG GGCCATTTCT ACTCTTCAAA GTAGTGATAT TCTTAATTTA
    ACAAAAGAAC AACCTCAGGC CAAAGCAGGC AATGGACAGA ACTCTTGTGG AGTAGAAGAT
    GTCCTTCAGC TTCTGCGTAT TCTATATATA GTTGCAAGTG ACCCTTATTC AAGAATATCC
    CAGGAAGATG GTGATGAACA GCCTCAGTTT ACTTTTCCAC CAGATGAATT CACTAGCAAA
    AAAATTACAA CAAAAATATT ACAGCAGATT GAGGAACCAT TGGCACTGGC AAGTGGGGCT
    CTGCCAGACT GGTGTGAACA ATTAACCAGC AAATGTCCTT TTCTAATACC ATTTGAAACT
    AGACAGCTTT ATTTCACATG TACAGCATTT GGCGCCTCAA GAGCAATAGT ATGGTTACAG
    AACCGACGTG AAGCCACTGT GGAGCGAACG AGAACCACAA GCAGTGTTAG GCGAGATGAC
    CCTGGAGAGT TTCGAGTTGG TCGTCTCAAG CATGAAAGAG TAAAAGTTCC ACGTGGCGAG
    TCACTGATGG AATGGGCTGA GAATGTCATG CAAATACATG CAGATCGGAA ATCAGTTCTT
    GAGGTTGAAT TTTTAGGAGA AGAAGGAACT GGCTTGGGAC CCACATTAGA GTTTTATGCT
    CTGGTGGCAG CAGAATTCCA GAGAACTGAC TTGGGAGCTT GGCTTTGTGA TGATAATTTT
    CCAGATGATG AATCTCGTCA CGTTGATCTT GGAGGTGGAT TGAAACCTCC TGGATATTAT
    GTGCAGAGGT CATGTGGACT GTTCACAGCA CCATTTCCAC AGGATAGTGA TGAGCTTGAA
    AGGATCACGA AACTGTTTCA TTTCCTTGGA ATTTTCTTGG CCAAATGCAT TCAAGACAAT
    AGACTTGTGG ACTTACCTAT TTCTAAACCT TTTTTTAAAC TTATGTGTAT GGGTGACATT
    AAAAGCAATA TGAGTAAACT GATTTATGAG TCACGAGGTG ATAGAGACTT ACACTGTACT
    GAAAGTCAGT CTGAAGCTTC TACAGAAGAA GGTCATGATT CACTCTCGGT AGGAAGCTTT
    GAAGAGGATT CAAAATCAGA ATTTATTCTT GATCCCCCTA AACCAAAACC CCCAGCTTGG
    TTTAATGGAA TTTTGACTTG GGAAGACTTT GAATTAGTAA ACCCACACAG AGCCAGATTT
    TTAAAAGAAA TTAAAGACCT TGCTATCAAG AGGCGCCAAA TTTTAAGCAA CAAAGGTCTT
    TCTGAAGATG AGAAGAACAC AAAATTACAG GAACTAGTGC TGAAGAATCC ATCAGGTTCT
    GGGCCTCCAC TTAGCATAGA GGATTTAGGT TTAAATTTCC AGTTTTGCCC TTCCTCAAGA
    ATATATGGTT TTACAGCTGT GGATCTCAAG CCAAGTGGTG AAGATGAGAT GATAACAATG
    GATAATGCAG AAGAATATGT GGATTTGATG TTTGACTTTT GTATGCATAC GGGTATTCAG
    AAACAAATGG AAGCCTTTAG AGATGGGTTT AATAAAGTTT TTCCAATGGA GAAATTAAGT
    TCCTTCAGCC ATGAAGAAGT CCAAATGATT CTTTGTGGAA ACCAGTCACC ATCCTGGGCA
    GCAGAGGATA TTATCAATTA CACTGAACCT AAGCTGGGTT ATACACGTGA CAGCCCTGGT
    TTCCTGAGGT TTGTGAGGGT TTTATGTGGC ATGTCTTCTG ATGAAAGGAA AGCATTCTTG
    CAGTTTACCA CTGGTTGTTC AACTCTACCC CCAGGTGGAC TGGCTAACCT GCATCCCAGG
    CTCACGGTTG TACGCAAGGT TGATGCTACT GATGCAAGCT ATCCATCAGT CAATACATGT
    GTGCATTACC TTAAGTTGCC TGAATATTCT TCCGAGGAGA TCATGAGAGA GCGCCTGCTA
    GCTGCTACAA TGGAGAAAGG CTTTCATCTC AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu66521
    Clone ID Related Accession (Same CDS sequence) XM_017021151.1 , XM_011536621.2 , XM_017021151.2
    Accession Version XM_017021151.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017021151.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCTG GTACTACATC AACAGTGACT ATGTCAACAT CCAGTGTTAC TAGCAGCAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTTGGTCAAT CTTTAAGTAA CACTTTAACC
    ACCAGCCTCA CATCAACTTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTATTGG CTGAGCTCGA TGATGATGAG
    GACTTACCTG AGCCAGATGA AGAAGATGAT GAGAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGGTTATGAT TCTGAGACGC CCATCCCTGC AACGTCGAGC TGGCTCCCGC
    TCTGATGTAA CGCATCATGC TGTTACCTCG CAGCTACCAC AGGTACCTGC TGGAGCAGGG
    AGCCGACCTA TTGGGGAGCA GGAAGAAGAA GAGTACGAAA CTAAAGGAGG ACGCCGGAGA
    ACATGGGATG ATGATTATGT GCTAAAGAGA CAGTTTTCTG CATTGGTTCC TGCTTTTGAT
    CCTAGACCTG GTCGTACTAA TGTCCAGCAG ACAACTGATC TAGAAATACC ACCCCCAGGG
    ACCCCTCATT CAGAGCTCTT GGAAGAAGTC GAATGTACTC CGTCACCTCG ATTAGCTCTC
    ACTTTGAAAG TAACAGGTCT TGGAACGACT CGTGAAGTTG AATTACCACT CACCAATTTC
    AGATCAACCA TCTTTTACTA TGTACAAAAA TTGCTTCAAT TGTCCTGTAA TGGCAATGTG
    AAATCAGATA AACTTAGGCG TATTTGGGAG CCCACATACA CAATCATGTA CAGAGAAATG
    AAGGATTCTG ATAAAGAAAA GGAAAATGGA AAAATGGGTT GCTGGTCTAT AGAGCATGTG
    GAGCAGTACC TTGGCACTGA TGAATTACCA AAGAATGACT TGATAACCTA CCTGCAGAAG
    AATGCAGACG CTGCTTTCCT GCGCCACTGG AAATTAACTG GCACTAATAA AAGTATTAGG
    AAAAACAGAA ATTGTTCTCA GCTCATAGCT GCATATAAGG ATTTTTGTGA GCATGGAACA
    AAGTCTGGGT TAAACCAGGG GGCCATTTCT ACTCTTCAAA GTAGTGATAT TCTTAATTTA
    ACAAAAGAAC AACCTCAGGC CAAAGCAGGC AATGGACAGA ACTCTTGTGG AGTAGAAGAT
    GTCCTTCAGC TTCTGCGTAT TCTATATATA GTTGCAAGTG ACCCTTATTC AAGAATATCC
    CAGGAAGATG GTGATGAACA GCCTCAGTTT ACTTTTCCAC CAGATGAATT CACTAGCAAA
    AAAATTACAA CAAAAATATT ACAGCAGATT GAGGAACCAT TGGCACTGGC AAGTGGGGCT
    CTGCCAGACT GGTGTGAACA ATTAACCAGC AAATGTCCTT TTCTAATACC ATTTGAAACT
    AGACAGCTTT ATTTCACATG TACAGCATTT GGCGCCTCAA GAGCAATAGT ATGGTTACAG
    AACCGACGTG AAGCCACTGT GGAGCGAACG AGAACCACAA GCAGTGTTAG GCGAGATGAC
    CCTGGAGAGT TTCGAGTTGG TCGTCTCAAG CATGAAAGAG TAAAAGTTCC ACGTGGCGAG
    TCACTGATGG AATGGGCTGA GAATGTCATG CAAATACATG CAGATCGGAA ATCAGTTCTT
    GAGGTTGAAT TTTTAGGAGA AGAAGGAACT GGCTTGGGAC CCACATTAGA GTTTTATGCT
    CTGGTGGCAG CAGAATTCCA GAGAACTGAC TTGGGAGCTT GGCTTTGTGA TGATAATTTT
    CCAGATGATG AATCTCGTCA CGTTGATCTT GGAGGTGGAT TGAAACCTCC TGGATATTAT
    GTGCAGAGGT CATGTGGACT GTTCACAGCA CCATTTCCAC AGGATAGTGA TGAGCTTGAA
    AGGATCACGA AACTGTTTCA TTTCCTTGGA ATTTTCTTGG CCAAATGCAT TCAAGACAAT
    AGACTTGTGG ACTTACCTAT TTCTAAACCT TTTTTTAAAC TTATGTGTAT GGGTGACATT
    AAAAGCAATA TGAGTAAACT GATTTATGAG TCACGAGGTG ATAGAGACTT ACACTGTACT
    GAAAGTCAGT CTGAAGCTTC TACAGAAGAA GGTCATGATT CACTCTCGGT AGGAAGCTTT
    GAAGAGGATT CAAAATCAGA ATTTATTCTT GATCCCCCTA AACCAAAACC CCCAGCTTGG
    TTTAATGGAA TTTTGACTTG GGAAGACTTT GAATTAGTAA ACCCACACAG AGCCAGATTT
    TTAAAAGAAA TTAAAGACCT TGCTATCAAG AGGCGCCAAA TTTTAAGCAA CAAAGGTCTT
    TCTGAAGATG AGAAGAACAC AAAATTACAG GAACTAGTGC TGAAGAATCC ATCAGGTTCT
    GGGCCTCCAC TTAGCATAGA GGATTTAGGT TTAAATTTCC AGTTTTGCCC TTCCTCAAGA
    ATATATGGTT TTACAGCTGT GGATCTCAAG CCAAGTGGTG AAGATGAGAT GATAACAATG
    GATAATGCAG AAGAATATGT GGATTTGATG TTTGACTTTT GTATGCATAC GGGTATTCAG
    AAACAAATGG AAGCCTTTAG AGATGGGTTT AATAAAGTTT TTCCAATGGA GAAATTAAGT
    TCCTTCAGCC ATGAAGAAGT CCAAATGATT CTTTGTGGAA ACCAGTCACC ATCCTGGGCA
    GCAGAGGATA TTATCAATTA CACTGAACCT AAGCTGGGTT ATACACGTGA CAGCCCTGGT
    TTCCTGAGGT TTGTGAGGGT TTTATGTGGC ATGTCTTCTG ATGAAAGGAA AGCATTCTTG
    CAGTTTACCA CTGGTTGTTC AACTCTACCC CCAGGTGGAC TGGCTAACCT GCATCCCAGG
    CTCACGGTTG TACGCAAGGT TGATGCTACT GATGCAAGCT ATCCATCAGT CAATACATGT
    GTGCATTACC TTAAGTTGCC TGAATATTCT TCCGAGGAGA TCATGAGAGA GCGCCTGCTA
    GCTGCTACAA TGGAGAAAGG CTTTCATCTC AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876640.1
    CDS530..7525
    Translation

    Target ORF information:

    RefSeq Version XM_017021151.2
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017021151.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCGGA ATGTACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCC
    GAACAGTGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA
    GACACCTTGC ACTCTGCTAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT TTCAGGACCA TCATCAGCAT GCAAACCAGG TCGCAGCACC
    ACAGGAGCTC CATCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACT
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGCCATAGTG AAGTGGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC
    TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT
    GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA
    AGGGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAACA AGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCGGAAGGTG GATCTGACAG TTCAGAAAGC
    CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TACGATGACC ATTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAACCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAAGCACTT
    CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCGT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA
    AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCAG CTGCCTATGG ACTTGTAGTA
    GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA
    AATGCCAGTG GACAAACACA CTACCTCTCA TTATCTGGAT TCGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    CGGCAGAGAC GTCTAGTACG TTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT
    ATCAGAGGCC TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT
    GTCACAGGAG AACTACACAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCTG GTACTACATC AACAGTGACT ATGTCAACAT CCAGTGTTAC TAGCAGCAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTTGGTCAAT CTTTAAGTAA CACTTTAACC
    ACCAGCCTCA CATCAACTTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTATTGG CTGAGCTCGA TGATGATGAG
    GACTTACCTG AGCCAGATGA AGAAGATGAT GAGAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGGTTATGAT TCTGAGACGC CCATCCCTGC AACGTCGAGC TGGCTCCCGC
    TCTGATGTAA CGCATCATGC TGTTACCTCG CAGCTACCAC AGGTACCTGC TGGAGCAGGG
    AGCCGACCTA TTGGGGAGCA GGAAGAAGAA GAGTACGAAA CTAAAGGAGG ACGCCGGAGA
    ACATGGGATG ATGATTATGT GCTAAAGAGA CAGTTTTCTG CATTGGTTCC TGCTTTTGAT
    CCTAGACCTG GTCGTACTAA TGTCCAGCAG ACAACTGATC TAGAAATACC ACCCCCAGGG
    ACCCCTCATT CAGAGCTCTT GGAAGAAGTC GAATGTACTC CGTCACCTCG ATTAGCTCTC
    ACTTTGAAAG TAACAGGTCT TGGAACGACT CGTGAAGTTG AATTACCACT CACCAATTTC
    AGATCAACCA TCTTTTACTA TGTACAAAAA TTGCTTCAAT TGTCCTGTAA TGGCAATGTG
    AAATCAGATA AACTTAGGCG TATTTGGGAG CCCACATACA CAATCATGTA CAGAGAAATG
    AAGGATTCTG ATAAAGAAAA GGAAAATGGA AAAATGGGTT GCTGGTCTAT AGAGCATGTG
    GAGCAGTACC TTGGCACTGA TGAATTACCA AAGAATGACT TGATAACCTA CCTGCAGAAG
    AATGCAGACG CTGCTTTCCT GCGCCACTGG AAATTAACTG GCACTAATAA AAGTATTAGG
    AAAAACAGAA ATTGTTCTCA GCTCATAGCT GCATATAAGG ATTTTTGTGA GCATGGAACA
    AAGTCTGGGT TAAACCAGGG GGCCATTTCT ACTCTTCAAA GTAGTGATAT TCTTAATTTA
    ACAAAAGAAC AACCTCAGGC CAAAGCAGGC AATGGACAGA ACTCTTGTGG AGTAGAAGAT
    GTCCTTCAGC TTCTGCGTAT TCTATATATA GTTGCAAGTG ACCCTTATTC AAGAATATCC
    CAGGAAGATG GTGATGAACA GCCTCAGTTT ACTTTTCCAC CAGATGAATT CACTAGCAAA
    AAAATTACAA CAAAAATATT ACAGCAGATT GAGGAACCAT TGGCACTGGC AAGTGGGGCT
    CTGCCAGACT GGTGTGAACA ATTAACCAGC AAATGTCCTT TTCTAATACC ATTTGAAACT
    AGACAGCTTT ATTTCACATG TACAGCATTT GGCGCCTCAA GAGCAATAGT ATGGTTACAG
    AACCGACGTG AAGCCACTGT GGAGCGAACG AGAACCACAA GCAGTGTTAG GCGAGATGAC
    CCTGGAGAGT TTCGAGTTGG TCGTCTCAAG CATGAAAGAG TAAAAGTTCC ACGTGGCGAG
    TCACTGATGG AATGGGCTGA GAATGTCATG CAAATACATG CAGATCGGAA ATCAGTTCTT
    GAGGTTGAAT TTTTAGGAGA AGAAGGAACT GGCTTGGGAC CCACATTAGA GTTTTATGCT
    CTGGTGGCAG CAGAATTCCA GAGAACTGAC TTGGGAGCTT GGCTTTGTGA TGATAATTTT
    CCAGATGATG AATCTCGTCA CGTTGATCTT GGAGGTGGAT TGAAACCTCC TGGATATTAT
    GTGCAGAGGT CATGTGGACT GTTCACAGCA CCATTTCCAC AGGATAGTGA TGAGCTTGAA
    AGGATCACGA AACTGTTTCA TTTCCTTGGA ATTTTCTTGG CCAAATGCAT TCAAGACAAT
    AGACTTGTGG ACTTACCTAT TTCTAAACCT TTTTTTAAAC TTATGTGTAT GGGTGACATT
    AAAAGCAATA TGAGTAAACT GATTTATGAG TCACGAGGTG ATAGAGACTT ACACTGTACT
    GAAAGTCAGT CTGAAGCTTC TACAGAAGAA GGTCATGATT CACTCTCGGT AGGAAGCTTT
    GAAGAGGATT CAAAATCAGA ATTTATTCTT GATCCCCCTA AACCAAAACC CCCAGCTTGG
    TTTAATGGAA TTTTGACTTG GGAAGACTTT GAATTAGTAA ACCCACACAG AGCCAGATTT
    TTAAAAGAAA TTAAAGACCT TGCTATCAAG AGGCGCCAAA TTTTAAGCAA CAAAGGTCTT
    TCTGAAGATG AGAAGAACAC AAAATTACAG GAACTAGTGC TGAAGAATCC ATCAGGTTCT
    GGGCCTCCAC TTAGCATAGA GGATTTAGGT TTAAATTTCC AGTTTTGCCC TTCCTCAAGA
    ATATATGGTT TTACAGCTGT GGATCTCAAG CCAAGTGGTG AAGATGAGAT GATAACAATG
    GATAATGCAG AAGAATATGT GGATTTGATG TTTGACTTTT GTATGCATAC GGGTATTCAG
    AAACAAATGG AAGCCTTTAG AGATGGGTTT AATAAAGTTT TTCCAATGGA GAAATTAAGT
    TCCTTCAGCC ATGAAGAAGT CCAAATGATT CTTTGTGGAA ACCAGTCACC ATCCTGGGCA
    GCAGAGGATA TTATCAATTA CACTGAACCT AAGCTGGGTT ATACACGTGA CAGCCCTGGT
    TTCCTGAGGT TTGTGAGGGT TTTATGTGGC ATGTCTTCTG ATGAAAGGAA AGCATTCTTG
    CAGTTTACCA CTGGTTGTTC AACTCTACCC CCAGGTGGAC TGGCTAACCT GCATCCCAGG
    CTCACGGTTG TACGCAAGGT TGATGCTACT GATGCAAGCT ATCCATCAGT CAATACATGT
    GTGCATTACC TTAAGTTGCC TGAATATTCT TCCGAGGAGA TCATGAGAGA GCGCCTGCTA
    GCTGCTACAA TGGAGAAAGG CTTTCATCTC AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu66522
    Clone ID Related Accession (Same CDS sequence) XM_011536622.1
    Accession Version XM_011536622.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6183bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGATTCATT TTTGCTCTGA AGCACTGTTA AAAGAAGTTT GTGATTCTGA TGTTGGTCAC 
    AATTTGCCTA CAATACTAGT GGAAATCACT GCAACTGTCC TGGATCAAGA GGATGATGAT
    GATGGCCACT TGCTGGCTTT GCAGATCATA AGGGATTTAG TAGATAAAGG TGGTGATATA
    TTTTTGGATC AGCTAGCCAG ACTTGGTGTA ATTAGCAAAG TGTCAACGTT GGCAGGTCCT
    TCCTCTGATG ATGAGAATGA AGAGGAATCA AAACCAGAAA AAGAAGATGA ACCACAGGAA
    GATGCTAAAG AATTGCAACA AGGTAAACCA TATCATTGGA GAGACTGGTC AATCATTAGG
    GGAAGGGACT GCTTATATAT TTGGAGTGAT GCAGCAGCCT TGGAATTATC TAATGGCAGT
    AATGGATGGT TCAGATTTAT CTTGGATGGA AAACTTGCCA CCATGTATTC AAGTGGTAGT
    CCGGAAGGTG GATCTGACAG TTCAGAAAGC CGAAGTGAAT TCTTAGAGAA GTTACAAAGA
    GCTCGAGGCC AAGTAAAGCC ATCTACTTCA AGTCAACCTA TACTGTCAGC ACCAGGACCC
    ACTAAACTTA CTGTAGGAAA TTGGTCACTG ACATGTTTGA AAGAAGGAGA AATTGCTATT
    CATAATTCAG ATGGTCAGCA AGCTACAATA TTGAAAGAAG ATTTACCTGG TTTTGTATTT
    GAATCTAATA GAGGAACCAA ACATTCATTT ACTGCAGAAA CTTCCCTGGG TTCAGAATTT
    GTGACTGGCT GGACTGGCAA AAGAGGCAGA AAACTGAAAT CTAAGTTAGA AAAAACAAAG
    CAAAAGGTAC GAACTATGGC TCGAGATTTA TACGATGACC ATTTTAAAGC TGTTGAAAGC
    ATGCCTCGTG GAGTAGTGGT AACACTCAGA AACATAGCAA CTCAGTTAGA GTCATCTTGG
    GAACTTCATA CAAATAGACA ATGTATTGAG AGTGAGAACA CTTGGAGAGA TTTAATGAAG
    ACAGCTTTAG AAAACCTAAT TGTACTTTTG AAGGATGAAA ACACAATTTC ACCATATGAA
    ATGTGTAGCA GTGGCTTGGT ACAAGCACTT CTTACTGTGT TAAACAATAG CATGGATTTG
    GATATGAAAC AAGATTGTAG TCAACTGGTA GAAAGAATAA ATGTTTTTAA AACTGCCTTT
    AGTGAAAATG AAGATGATGA AAGTCGACCA GCAGTTGCGT TAATTCGAAA GTTAATAGCT
    GTACTAGAAT CTATTGAACG TCTACCTCTC CATTTGTATG ATACACCAGG ATCCACATAT
    AACCTCCAGA TACTTACAAG GAGATTACGA TTTCGGTTGG AACGTGCACC TGGTGAAACT
    GCATTGATTG ACAGGACTGG CAGAATGTTG AAGATGGAAC CTTTGGCTAC AGTTGAATCT
    CTGGAACAGT ACCTTTTGAA AATGGTAGCA AAACAGTGGT ATGATTTTGA CCGATCTTCA
    TTTGTTTTTG TTCGAAAATT AAGAGAAGGA CAAAATTTTA TATTTCGGCA CCAGCATGAT
    TTTGATGAAA ATGGAATCAT TTACTGGATT GGAACAAATG CAAAAACTGC TTATGAATGG
    GTAAATCCAG CTGCCTATGG ACTTGTAGTA GTAACGTCAT CAGAAGGAAG AAATCTACCT
    TATGGCCGCT TAGAAGACAT ACTAAGTCGT GATAATTCAG CTTTAAATTG TCATAGCAAT
    GATGATAAGA ATGCCTGGTT TGCCATAGAT CTGGGTCTCT GGGTGATACC ATCAGCATAT
    ACACTTCGTC ATGCTCGTGG TTATGGAAGG TCTGCACTGA GAAATTGGGT TTTCCAGGTA
    TCCAAAGATG GACAGAACTG GACTTCTTTG TATACCCATG TTGATGACTG CAGTCTCAAT
    GAACCAGGGT CAACTGCAAC TTGGCCTCTT GATCCACCAA AGGATGAGAA ACAAGGGTGG
    AGACATGTGA GAATTAAACA GATGGGGAAA AATGCCAGTG GACAAACACA CTACCTCTCA
    TTATCTGGAT TCGAACTTTA TGGCACTGTA AATGGAGTAT GTGAAGATCA GCTAGGGAAA
    GCAGCTAAAG AAGCAGAAGC TAATCTTAGA CGGCAGAGAC GTCTAGTACG TTCCCAGGTT
    CTGAAATACA TGGTTCCAGG AGCTCGTGTT ATCAGAGGCC TGGATTGGAA ATGGCGAGAT
    CAGGATGGCA GCCCACAGGG AGAAGGCACT GTCACAGGAG AACTACACAA TGGCTGGATT
    GATGTCACCT GGGATGCTGG TGGCTCAAAC TCTTACCGTA TGGGCGCAGA AGGAAAATTT
    GACCTCAAGC TTGCACCAGG GTACGACCCT GATACAGTGG CATCACCCAA ACCTGTTTCA
    TCCACTGTTT CAGGCACAAC GCAATCATGG AGCAGCTTGG TGAAAAACAA CTGTCCAGAC
    AAGACATCTG CTGCTGCAGG CTCCTCAAGT AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC
    GTGGCCAGTA GCAGCGACAT CAGCTTGGGT TCGACCAAAA CGGAACGGAG ATCAGAAATT
    GTAATGGAAC ACAGTATAGT TTCAGGAGCT GATGTCCATG AACCAATTGT TGTTCTTTCA
    TCTGCTGAAA ACGTCCCTCA AACAGAAGTA GGGTCATCTT CCAGTGCAAG CACCAGCACC
    TTAACAGCGG AAACGGGAAG TGAAAATGCT GAAAGGAAGT TAGGCCCTGA TAGTTCTGTT
    CGTACTCCTG GGGAGTCTAG TGCAATATCC ATGGGAATTG TCAGTGTTAG TTCTCCTGAT
    GTTAGTTCAG TATCTGAATT AACTAATAAA GAAGCAGCTT CACAACGACC TCTTAGCTCT
    TCAGCAAGTA ACAGACTGTC AGTGAGTTCT TTGTTGGCTG CTGGGGCCCC TATGAGCTCT
    AGTGCAAGTG TACCTAACCT GTCCTCAAGA GAAACATCTA GCTTGGAGAG TTTTGTAAGG
    AGAGTGGCAA ACATAGCACG GACTAATGCC ACGAACAACA TGAATCTAAG CCGAAGCAGC
    AGTGATAACA ACACTAATAC TTTGGGGAGG AATGTGATGA GCACAGCAAC TTCTCCTCTT
    ATGGGTGCTC AGAGTTTCCC TAATTTGACC ACACCTGGTA CTACATCAAC AGTGACTATG
    TCAACATCCA GTGTTACTAG CAGCAGCAAT GTAGCTACAG CAACAACAGT TTTATCAGTT
    GGTCAATCTT TAAGTAACAC TTTAACCACC AGCCTCACAT CAACTTCCAG TGAGAGTGAC
    ACAGGTCAGG AAGCAGAATA TTCCTTATAT GATTTCCTTG ATAGCTGCCG TGCCAGTACT
    CTATTGGCTG AGCTCGATGA TGATGAGGAC TTACCTGAGC CAGATGAAGA AGATGATGAG
    AATGAAGATG ACAATCAGGA GGACCAAGAA TACGAGGAGG TTATGATTCT GAGACGCCCA
    TCCCTGCAAC GTCGAGCTGG CTCCCGCTCT GATGTAACGC ATCATGCTGT TACCTCGCAG
    CTACCACAGG TACCTGCTGG AGCAGGGAGC CGACCTATTG GGGAGCAGGA AGAAGAAGAG
    TACGAAACTA AAGGAGGACG CCGGAGAACA TGGGATGATG ATTATGTGCT AAAGAGACAG
    TTTTCTGCAT TGGTTCCTGC TTTTGATCCT AGACCTGGTC GTACTAATGT CCAGCAGACA
    ACTGATCTAG AAATACCACC CCCAGGGACC CCTCATTCAG AGCTCTTGGA AGAAGTCGAA
    TGTACTCCGT CACCTCGATT AGCTCTCACT TTGAAAGTAA CAGGTCTTGG AACGACTCGT
    GAAGTTGAAT TACCACTCAC CAATTTCAGA TCAACCATCT TTTACTATGT ACAAAAATTG
    CTTCAATTGT CCTGTAATGG CAATGTGAAA TCAGATAAAC TTAGGCGTAT TTGGGAGCCC
    ACATACACAA TCATGTACAG AGAAATGAAG GATTCTGATA AAGAAAAGGA AAATGGAAAA
    ATGGGTTGCT GGTCTATAGA GCATGTGGAG CAGTACCTTG GCACTGATGA ATTACCAAAG
    AATGACTTGA TAACCTACCT GCAGAAGAAT GCAGACGCTG CTTTCCTGCG CCACTGGAAA
    TTAACTGGCA CTAATAAAAG TATTAGGAAA AACAGAAATT GTTCTCAGCT CATAGCTGCA
    TATAAGGATT TTTGTGAGCA TGGAACAAAG TCTGGGTTAA ACCAGGGGGC CATTTCTACT
    CTTCAAAGTA GTGATATTCT TAATTTAACA AAAGAACAAC CTCAGGCCAA AGCAGGCAAT
    GGACAGAACT CTTGTGGAGT AGAAGATGTC CTTCAGCTTC TGCGTATTCT ATATATAGTT
    GCAAGTGACC CTTATTCAAG AATATCCCAG GAAGATGGTG ATGAACAGCC TCAGTTTACT
    TTTCCACCAG ATGAATTCAC TAGCAAAAAA ATTACAACAA AAATATTACA GCAGATTGAG
    GAACCATTGG CACTGGCAAG TGGGGCTCTG CCAGACTGGT GTGAACAATT AACCAGCAAA
    TGTCCTTTTC TAATACCATT TGAAACTAGA CAGCTTTATT TCACATGTAC AGCATTTGGC
    GCCTCAAGAG CAATAGTATG GTTACAGAAC CGACGTGAAG CCACTGTGGA GCGAACGAGA
    ACCACAAGCA GTGTTAGGCG AGATGACCCT GGAGAGTTTC GAGTTGGTCG TCTCAAGCAT
    GAAAGAGTAA AAGTTCCACG TGGCGAGTCA CTGATGGAAT GGGCTGAGAA TGTCATGCAA
    ATACATGCAG ATCGGAAATC AGTTCTTGAG GTTGAATTTT TAGGAGAAGA AGGAACTGGC
    TTGGGACCCA CATTAGAGTT TTATGCTCTG GTGGCAGCAG AATTCCAGAG AACTGACTTG
    GGAGCTTGGC TTTGTGATGA TAATTTTCCA GATGATGAAT CTCGTCACGT TGATCTTGGA
    GGTGGATTGA AACCTCCTGG ATATTATGTG CAGAGGTCAT GTGGACTGTT CACAGCACCA
    TTTCCACAGG ATAGTGATGA GCTTGAAAGG ATCACGAAAC TGTTTCATTT CCTTGGAATT
    TTCTTGGCCA AATGCATTCA AGACAATAGA CTTGTGGACT TACCTATTTC TAAACCTTTT
    TTTAAACTTA TGTGTATGGG TGACATTAAA AGCAATATGA GTAAACTGAT TTATGAGTCA
    CGAGGTGATA GAGACTTACA CTGTACTGAA AGTCAGTCTG AAGCTTCTAC AGAAGAAGGT
    CATGATTCAC TCTCGGTAGG AAGCTTTGAA GAGGATTCAA AATCAGAATT TATTCTTGAT
    CCCCCTAAAC CAAAACCCCC AGCTTGGTTT AATGGAATTT TGACTTGGGA AGACTTTGAA
    TTAGTAAACC CACACAGAGC CAGATTTTTA AAAGAAATTA AAGACCTTGC TATCAAGAGG
    CGCCAAATTT TAAGCAACAA AGGTCTTTCT GAAGATGAGA AGAACACAAA ATTACAGGAA
    CTAGTGCTGA AGAATCCATC AGGTTCTGGG CCTCCACTTA GCATAGAGGA TTTAGGTTTA
    AATTTCCAGT TTTGCCCTTC CTCAAGAATA TATGGTTTTA CAGCTGTGGA TCTCAAGCCA
    AGTGGTGAAG ATGAGATGAT AACAATGGAT AATGCAGAAG AATATGTGGA TTTGATGTTT
    GACTTTTGTA TGCATACGGG TATTCAGAAA CAAATGGAAG CCTTTAGAGA TGGGTTTAAT
    AAAGTTTTTC CAATGGAGAA ATTAAGTTCC TTCAGCCATG AAGAAGTCCA AATGATTCTT
    TGTGGAAACC AGTCACCATC CTGGGCAGCA GAGGATATTA TCAATTACAC TGAACCTAAG
    CTGGGTTATA CACGTGACAG CCCTGGTTTC CTGAGGTTTG TGAGGGTTTT ATGTGGCATG
    TCTTCTGATG AAAGGAAAGC ATTCTTGCAG TTTACCACTG GTTGTTCAAC TCTACCCCCA
    GGTGGACTGG CTAACCTGCA TCCCAGGCTC ACGGTTGTAC GCAAGGTTGA TGCTACTGAT
    GCAAGCTATC CATCAGTCAA TACATGTGTG CATTACCTTA AGTTGCCTGA ATATTCTTCC
    GAGGAGATCA TGAGAGAGCG CCTGCTAGCT GCTACAATGG AGAAAGGCTT TCATCTCAAT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011534924.1
    CDS105..6287
    Translation

    Target ORF information:

    RefSeq Version XM_011536622.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011536622.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGATTCATT TTTGCTCTGA AGCACTGTTA AAAGAAGTTT GTGATTCTGA TGTTGGTCAC 
    AATTTGCCTA CAATACTAGT GGAAATCACT GCAACTGTCC TGGATCAAGA GGATGATGAT
    GATGGCCACT TGCTGGCTTT GCAGATCATA AGGGATTTAG TAGATAAAGG TGGTGATATA
    TTTTTGGATC AGCTAGCCAG ACTTGGTGTA ATTAGCAAAG TGTCAACGTT GGCAGGTCCT
    TCCTCTGATG ATGAGAATGA AGAGGAATCA AAACCAGAAA AAGAAGATGA ACCACAGGAA
    GATGCTAAAG AATTGCAACA AGGTAAACCA TATCATTGGA GAGACTGGTC AATCATTAGG
    GGAAGGGACT GCTTATATAT TTGGAGTGAT GCAGCAGCCT TGGAATTATC TAATGGCAGT
    AATGGATGGT TCAGATTTAT CTTGGATGGA AAACTTGCCA CCATGTATTC AAGTGGTAGT
    CCGGAAGGTG GATCTGACAG TTCAGAAAGC CGAAGTGAAT TCTTAGAGAA GTTACAAAGA
    GCTCGAGGCC AAGTAAAGCC ATCTACTTCA AGTCAACCTA TACTGTCAGC ACCAGGACCC
    ACTAAACTTA CTGTAGGAAA TTGGTCACTG ACATGTTTGA AAGAAGGAGA AATTGCTATT
    CATAATTCAG ATGGTCAGCA AGCTACAATA TTGAAAGAAG ATTTACCTGG TTTTGTATTT
    GAATCTAATA GAGGAACCAA ACATTCATTT ACTGCAGAAA CTTCCCTGGG TTCAGAATTT
    GTGACTGGCT GGACTGGCAA AAGAGGCAGA AAACTGAAAT CTAAGTTAGA AAAAACAAAG
    CAAAAGGTAC GAACTATGGC TCGAGATTTA TACGATGACC ATTTTAAAGC TGTTGAAAGC
    ATGCCTCGTG GAGTAGTGGT AACACTCAGA AACATAGCAA CTCAGTTAGA GTCATCTTGG
    GAACTTCATA CAAATAGACA ATGTATTGAG AGTGAGAACA CTTGGAGAGA TTTAATGAAG
    ACAGCTTTAG AAAACCTAAT TGTACTTTTG AAGGATGAAA ACACAATTTC ACCATATGAA
    ATGTGTAGCA GTGGCTTGGT ACAAGCACTT CTTACTGTGT TAAACAATAG CATGGATTTG
    GATATGAAAC AAGATTGTAG TCAACTGGTA GAAAGAATAA ATGTTTTTAA AACTGCCTTT
    AGTGAAAATG AAGATGATGA AAGTCGACCA GCAGTTGCGT TAATTCGAAA GTTAATAGCT
    GTACTAGAAT CTATTGAACG TCTACCTCTC CATTTGTATG ATACACCAGG ATCCACATAT
    AACCTCCAGA TACTTACAAG GAGATTACGA TTTCGGTTGG AACGTGCACC TGGTGAAACT
    GCATTGATTG ACAGGACTGG CAGAATGTTG AAGATGGAAC CTTTGGCTAC AGTTGAATCT
    CTGGAACAGT ACCTTTTGAA AATGGTAGCA AAACAGTGGT ATGATTTTGA CCGATCTTCA
    TTTGTTTTTG TTCGAAAATT AAGAGAAGGA CAAAATTTTA TATTTCGGCA CCAGCATGAT
    TTTGATGAAA ATGGAATCAT TTACTGGATT GGAACAAATG CAAAAACTGC TTATGAATGG
    GTAAATCCAG CTGCCTATGG ACTTGTAGTA GTAACGTCAT CAGAAGGAAG AAATCTACCT
    TATGGCCGCT TAGAAGACAT ACTAAGTCGT GATAATTCAG CTTTAAATTG TCATAGCAAT
    GATGATAAGA ATGCCTGGTT TGCCATAGAT CTGGGTCTCT GGGTGATACC ATCAGCATAT
    ACACTTCGTC ATGCTCGTGG TTATGGAAGG TCTGCACTGA GAAATTGGGT TTTCCAGGTA
    TCCAAAGATG GACAGAACTG GACTTCTTTG TATACCCATG TTGATGACTG CAGTCTCAAT
    GAACCAGGGT CAACTGCAAC TTGGCCTCTT GATCCACCAA AGGATGAGAA ACAAGGGTGG
    AGACATGTGA GAATTAAACA GATGGGGAAA AATGCCAGTG GACAAACACA CTACCTCTCA
    TTATCTGGAT TCGAACTTTA TGGCACTGTA AATGGAGTAT GTGAAGATCA GCTAGGGAAA
    GCAGCTAAAG AAGCAGAAGC TAATCTTAGA CGGCAGAGAC GTCTAGTACG TTCCCAGGTT
    CTGAAATACA TGGTTCCAGG AGCTCGTGTT ATCAGAGGCC TGGATTGGAA ATGGCGAGAT
    CAGGATGGCA GCCCACAGGG AGAAGGCACT GTCACAGGAG AACTACACAA TGGCTGGATT
    GATGTCACCT GGGATGCTGG TGGCTCAAAC TCTTACCGTA TGGGCGCAGA AGGAAAATTT
    GACCTCAAGC TTGCACCAGG GTACGACCCT GATACAGTGG CATCACCCAA ACCTGTTTCA
    TCCACTGTTT CAGGCACAAC GCAATCATGG AGCAGCTTGG TGAAAAACAA CTGTCCAGAC
    AAGACATCTG CTGCTGCAGG CTCCTCAAGT AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC
    GTGGCCAGTA GCAGCGACAT CAGCTTGGGT TCGACCAAAA CGGAACGGAG ATCAGAAATT
    GTAATGGAAC ACAGTATAGT TTCAGGAGCT GATGTCCATG AACCAATTGT TGTTCTTTCA
    TCTGCTGAAA ACGTCCCTCA AACAGAAGTA GGGTCATCTT CCAGTGCAAG CACCAGCACC
    TTAACAGCGG AAACGGGAAG TGAAAATGCT GAAAGGAAGT TAGGCCCTGA TAGTTCTGTT
    CGTACTCCTG GGGAGTCTAG TGCAATATCC ATGGGAATTG TCAGTGTTAG TTCTCCTGAT
    GTTAGTTCAG TATCTGAATT AACTAATAAA GAAGCAGCTT CACAACGACC TCTTAGCTCT
    TCAGCAAGTA ACAGACTGTC AGTGAGTTCT TTGTTGGCTG CTGGGGCCCC TATGAGCTCT
    AGTGCAAGTG TACCTAACCT GTCCTCAAGA GAAACATCTA GCTTGGAGAG TTTTGTAAGG
    AGAGTGGCAA ACATAGCACG GACTAATGCC ACGAACAACA TGAATCTAAG CCGAAGCAGC
    AGTGATAACA ACACTAATAC TTTGGGGAGG AATGTGATGA GCACAGCAAC TTCTCCTCTT
    ATGGGTGCTC AGAGTTTCCC TAATTTGACC ACACCTGGTA CTACATCAAC AGTGACTATG
    TCAACATCCA GTGTTACTAG CAGCAGCAAT GTAGCTACAG CAACAACAGT TTTATCAGTT
    GGTCAATCTT TAAGTAACAC TTTAACCACC AGCCTCACAT CAACTTCCAG TGAGAGTGAC
    ACAGGTCAGG AAGCAGAATA TTCCTTATAT GATTTCCTTG ATAGCTGCCG TGCCAGTACT
    CTATTGGCTG AGCTCGATGA TGATGAGGAC TTACCTGAGC CAGATGAAGA AGATGATGAG
    AATGAAGATG ACAATCAGGA GGACCAAGAA TACGAGGAGG TTATGATTCT GAGACGCCCA
    TCCCTGCAAC GTCGAGCTGG CTCCCGCTCT GATGTAACGC ATCATGCTGT TACCTCGCAG
    CTACCACAGG TACCTGCTGG AGCAGGGAGC CGACCTATTG GGGAGCAGGA AGAAGAAGAG
    TACGAAACTA AAGGAGGACG CCGGAGAACA TGGGATGATG ATTATGTGCT AAAGAGACAG
    TTTTCTGCAT TGGTTCCTGC TTTTGATCCT AGACCTGGTC GTACTAATGT CCAGCAGACA
    ACTGATCTAG AAATACCACC CCCAGGGACC CCTCATTCAG AGCTCTTGGA AGAAGTCGAA
    TGTACTCCGT CACCTCGATT AGCTCTCACT TTGAAAGTAA CAGGTCTTGG AACGACTCGT
    GAAGTTGAAT TACCACTCAC CAATTTCAGA TCAACCATCT TTTACTATGT ACAAAAATTG
    CTTCAATTGT CCTGTAATGG CAATGTGAAA TCAGATAAAC TTAGGCGTAT TTGGGAGCCC
    ACATACACAA TCATGTACAG AGAAATGAAG GATTCTGATA AAGAAAAGGA AAATGGAAAA
    ATGGGTTGCT GGTCTATAGA GCATGTGGAG CAGTACCTTG GCACTGATGA ATTACCAAAG
    AATGACTTGA TAACCTACCT GCAGAAGAAT GCAGACGCTG CTTTCCTGCG CCACTGGAAA
    TTAACTGGCA CTAATAAAAG TATTAGGAAA AACAGAAATT GTTCTCAGCT CATAGCTGCA
    TATAAGGATT TTTGTGAGCA TGGAACAAAG TCTGGGTTAA ACCAGGGGGC CATTTCTACT
    CTTCAAAGTA GTGATATTCT TAATTTAACA AAAGAACAAC CTCAGGCCAA AGCAGGCAAT
    GGACAGAACT CTTGTGGAGT AGAAGATGTC CTTCAGCTTC TGCGTATTCT ATATATAGTT
    GCAAGTGACC CTTATTCAAG AATATCCCAG GAAGATGGTG ATGAACAGCC TCAGTTTACT
    TTTCCACCAG ATGAATTCAC TAGCAAAAAA ATTACAACAA AAATATTACA GCAGATTGAG
    GAACCATTGG CACTGGCAAG TGGGGCTCTG CCAGACTGGT GTGAACAATT AACCAGCAAA
    TGTCCTTTTC TAATACCATT TGAAACTAGA CAGCTTTATT TCACATGTAC AGCATTTGGC
    GCCTCAAGAG CAATAGTATG GTTACAGAAC CGACGTGAAG CCACTGTGGA GCGAACGAGA
    ACCACAAGCA GTGTTAGGCG AGATGACCCT GGAGAGTTTC GAGTTGGTCG TCTCAAGCAT
    GAAAGAGTAA AAGTTCCACG TGGCGAGTCA CTGATGGAAT GGGCTGAGAA TGTCATGCAA
    ATACATGCAG ATCGGAAATC AGTTCTTGAG GTTGAATTTT TAGGAGAAGA AGGAACTGGC
    TTGGGACCCA CATTAGAGTT TTATGCTCTG GTGGCAGCAG AATTCCAGAG AACTGACTTG
    GGAGCTTGGC TTTGTGATGA TAATTTTCCA GATGATGAAT CTCGTCACGT TGATCTTGGA
    GGTGGATTGA AACCTCCTGG ATATTATGTG CAGAGGTCAT GTGGACTGTT CACAGCACCA
    TTTCCACAGG ATAGTGATGA GCTTGAAAGG ATCACGAAAC TGTTTCATTT CCTTGGAATT
    TTCTTGGCCA AATGCATTCA AGACAATAGA CTTGTGGACT TACCTATTTC TAAACCTTTT
    TTTAAACTTA TGTGTATGGG TGACATTAAA AGCAATATGA GTAAACTGAT TTATGAGTCA
    CGAGGTGATA GAGACTTACA CTGTACTGAA AGTCAGTCTG AAGCTTCTAC AGAAGAAGGT
    CATGATTCAC TCTCGGTAGG AAGCTTTGAA GAGGATTCAA AATCAGAATT TATTCTTGAT
    CCCCCTAAAC CAAAACCCCC AGCTTGGTTT AATGGAATTT TGACTTGGGA AGACTTTGAA
    TTAGTAAACC CACACAGAGC CAGATTTTTA AAAGAAATTA AAGACCTTGC TATCAAGAGG
    CGCCAAATTT TAAGCAACAA AGGTCTTTCT GAAGATGAGA AGAACACAAA ATTACAGGAA
    CTAGTGCTGA AGAATCCATC AGGTTCTGGG CCTCCACTTA GCATAGAGGA TTTAGGTTTA
    AATTTCCAGT TTTGCCCTTC CTCAAGAATA TATGGTTTTA CAGCTGTGGA TCTCAAGCCA
    AGTGGTGAAG ATGAGATGAT AACAATGGAT AATGCAGAAG AATATGTGGA TTTGATGTTT
    GACTTTTGTA TGCATACGGG TATTCAGAAA CAAATGGAAG CCTTTAGAGA TGGGTTTAAT
    AAAGTTTTTC CAATGGAGAA ATTAAGTTCC TTCAGCCATG AAGAAGTCCA AATGATTCTT
    TGTGGAAACC AGTCACCATC CTGGGCAGCA GAGGATATTA TCAATTACAC TGAACCTAAG
    CTGGGTTATA CACGTGACAG CCCTGGTTTC CTGAGGTTTG TGAGGGTTTT ATGTGGCATG
    TCTTCTGATG AAAGGAAAGC ATTCTTGCAG TTTACCACTG GTTGTTCAAC TCTACCCCCA
    GGTGGACTGG CTAACCTGCA TCCCAGGCTC ACGGTTGTAC GCAAGGTTGA TGCTACTGAT
    GCAAGCTATC CATCAGTCAA TACATGTGTG CATTACCTTA AGTTGCCTGA ATATTCTTCC
    GAGGAGATCA TGAGAGAGCG CCTGCTAGCT GCTACAATGG AGAAAGGCTT TCATCTCAAT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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