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NIPBL cDNA ORF clone, Homo sapiens(Human)

Gene Symbol NIPBL
Entrez Gene ID 25836
Full Name NIPBL, cohesin loading factor
Synonyms CDLS,CDLS1,IDN3,IDN3-B,Scc2
General protein information
Preferred Names

NIPBL, cohesin loading factor

Names

nipped-B-like protein
Nipped-B homolog
SCC2 homolog
delangin
sister chromatid cohesion 2 homolog

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

5

5p13.2

Summary This gene encodes the homolog of the Drosophila melanogaster Nipped-B gene product and fungal Scc2-type sister chromatid cohesion proteins. The Drosophila protein facilitates enhancer-promoter communication of remote enhancers and plays a role in developmental regulation. It is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair. The human protein has a bipartite nuclear targeting sequence and a putative HEAT repeat. Condensins, cohesins and other complexes with chromosome-related functions also contain HEAT repeats. Mutations in this gene result in Cornelia de Lange syndrome, a disorder characterized by dysmorphic facial features, growth delay, limb reduction defects, and mental retardation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:

608667

Disorder Html: Cornelia de Lange syndrome 1, 122470 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_006714468.2 XP_006714531.1 nipped-B-like protein isoform X2
XM_005248282.4 XP_005248339.3 nipped-B-like protein isoform X5
XM_006714467.2 XP_006714530.1 nipped-B-like protein isoform X1
NM_133433.3 NP_597677.2 nipped-B-like protein isoform A
XM_017009329.1 XP_016864818.1 nipped-B-like protein isoform X4
XM_011514015.1 XP_011512317.1 nipped-B-like protein isoform X6
NM_015384.4 NP_056199.2 nipped-B-like protein isoform B
XM_005248280.3 XP_005248337.1 nipped-B-like protein isoform X3
XM_017009330.1 XP_016864819.1 nipped-B-like protein isoform X7
XM_017009331.1 XP_016864820.1 nipped-B-like protein isoform X8



Rattus norvegicus (Norway rat) Nipbl XP_003749266.1
Drosophila melanogaster (fruit fly) Nipped-B NP_001163052.1
Macaca mulatta (rhesus monkey) NIPBL XP_001096575.1
Homo sapiens (human) NIPBL NP_597677.2
Canis lupus familiaris (dog) NIPBL XP_005619439.1
Bos taurus (cattle) NIPBL NP_001193513.1
Mus musculus (house mouse) Nipbl NP_081983.2
Gallus gallus (chicken) LOC427025 NP_001244277.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP009290 XP_320088.4
Pan troglodytes (chimpanzee) NIPBL XP_001146090.1
Gallus gallus (chicken) LOC427439 XP_425012.3
Danio rerio (zebrafish) nipblb NP_001154919.2
Xenopus tropicalis (tropical clawed frog) nipbl XP_002938229.2


Related articles in PubMed

mRNA Quantification of NIPBL Isoforms A and B in Adult and Fetal Human Tissues, and a Potentially Pathological Variant Affecting Only Isoform A in Two Patients with Cornelia de Lange Syndrome.
Puisac B, Teresa-Rodrigo ME, Hernández-Marcos M, Baquero-Montoya C, Gil-Rodríguez MC, Visnes T, Bot C, Gómez-Puertas P, Kaiser FJ, Ramos FJ, Ström L, Pié J
International journal of molecular sciences18(3)(2017 Feb)

A series of 38 novel germline and somatic mutations of NIPBL in Cornelia de Lange syndrome.
Nizon M, Henry M, Michot C, Baumann C, Bazin A, Bessières B, Blesson S, Cordier-Alex MP, David A, Delahaye-Duriez A, Delezoïde AL, Dieux-Coeslier A, Doco-Fenzy M, Faivre L, Goldenberg A, Layet V, Loget P, Marlin S, Martinovic J, Odent S, Pasquier L, Plessis G, Prieur F, Putoux A, Rio M, Testard H, Bonnefont JP, Cormier-Daire V
Clinical genetics89(5)584-9(2016 May)

NIPBL Controls RNA Biogenesis to Prevent Activation of the Stress Kinase PKR.
Yuen KC, Xu B, Krantz ID, Gerton JL
Cell reports14(1)93-102(2016 Jan)

Identification and Functional Characterization of Two Intronic NIPBL Mutations in Two Patients with Cornelia de Lange Syndrome.
Teresa-Rodrigo ME, Eckhold J, Puisac B, Pozojevic J, Parenti I, Baquero-Montoya C, Gil-Rodríguez MC, Braunholz D, Dalski A, Hernández-Marcos M, Ayerza A, Bernal ML, Ramos FJ, Wieczorek D, Gillessen-Kaesbach G, Pié J, Kaiser FJ
BioMed research international20168742939(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study identifies four likely Tourette disorder risk genes with multiple de novo damaging variants in unrelated probands: WWC1 (WW and C2 domain containing 1), CELSR3 (Cadherin EGF LAG seven-pass G-type receptor 3), NIPBL (Nipped-B-like), and FN1 (fibronectin 1).
Title: De Novo Coding Variants Are Strongly Associated with Tourette Disorder.

NIPBL has evolved a sophisticated response to damaged DNA that is influenced by the form of damage, suggesting a highly dynamic role for NIPBL in maintaining genomic stability.
Title: Independent mechanisms recruit the cohesin loader protein NIPBL to sites of DNA damage.

Pathological variant specific of the isoform A of NIPBL was identified in two patients with Cornelia de Lange Syndrome.
Title: mRNA Quantification of NIPBL Isoforms A and B in Adult and Fetal Human Tissues, and a Potentially Pathological Variant Affecting Only Isoform A in Two Patients with Cornelia de Lange Syndrome.

This study provides insight into the molecular pathology of Cornelia de Lange syndrome by establishing a relationship between NIPBL and HDAC8 mutations and PKR activation.
Title: NIPBL Controls RNA Biogenesis to Prevent Activation of the Stress Kinase PKR.

Subsequent quantitative PCR analysis demonstrated a 30% decrease of the total NIPBL mRNA levels associated with the frameshift transcript
Title: Identification and Functional Characterization of Two Intronic NIPBL Mutations in Two Patients with Cornelia de Lange Syndrome.

The following NIPBL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NIPBL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu95814 XM_017009330.1
Latest version!
Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 22-24 $1091.30
$1559.00
OHu95812 XM_017009329.1
Latest version!
Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 29-31 $1301.30
$1859.00
OHu95813 XM_005248282.4
Latest version!
Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 29-31 $1301.30
$1859.00
OHu95815 XM_017009331.1
Latest version!
Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 29-31 $1091.30
$1559.00
OHu26171 NM_133433.3
Latest version!
Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant A, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $923.30
$1319.00
OHu26399 NM_015384.4
Latest version!
Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant B, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $923.30
$1319.00
OHu39306 XM_006714467.2
Latest version!
Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1511.30
$2159.00
OHu39307 XM_006714468.2
Latest version!
Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1511.30
$2159.00
OHu39308 XM_005248280.3
Latest version!
Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1511.30
$2159.00
OHu66530 XM_011514015.1
Latest version!
Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1301.30
$1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
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All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu95814
Clone ID Related Accession (Same CDS sequence) XM_017009330.1
Accession Version XM_017009330.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6798bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product nipped-B-like protein isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
ATGGTTAGCA TTGATCTTCA TCAGGCAGGA AGAGTGGACT CTCAGGCTTC TATAACTCAG 
GATTCAGACT CCATAAAAAA GCCTGAAGAA ATCAAACAAT GTAATGATGC ACCTGTTTCT
GTTCTTCAGG AAGATATTGT TGGAAGTCTT AAATCTACAC CAGAAAACCA TCCTGAGACA
CCTAAAAAAA AGTCTGATCC TGAGCTTTCA AAGAGTGAAA TGAAACAAAG TGAAAGTAGA
TTAGCAGAAT CTAAACCAAA TGAAAACCGA TTGGTGGAGA CAAAATCAAG TGAAAATAAG
TTAGAAACTA AAGTTGAGAC CCAAACAGAA GAACTTAAAC AGAATGAGAG CAGAACAACT
GAATGCAAAC AAAACGAGAG CACCATAGTT GAGCCTAAAC AAAATGAAAA TAGACTGTCT
GACACAAAAC CAAATGACAA CAAACAAAAT AATGGCAGAT CAGAAACAAC AAAATCAAGG
CCTGAAACCC CAAAGCAAAA GGGTGAAAGC CGGCCTGAGA CTCCAAAACA AAAGAGTGAT
GGGCATCCTG AAACCCCAAA ACAGAAGGGT GATGGAAGGC CTGAAACTCC AAAGCAAAAA
GGTGAGAGCC GCCCTGAAAC TCCAAAGCAA AAAAATGAAG GGCGACCTGA AACACCAAAA
CACAGGCATG ACAATAGGAG GGATTCTGGA AAGCCATCTA CAGAGAAAAA ACCTGAAGTG
TCTAAACATA AACAAGATAC TAAATCTGAC TCACCTCGGT TAAAATCAGA ACGAGCTGAA
GCCTTAAAGC AGAGACCTGA TGGGCGATCT GTTTCTGAGT CACTAAGACG TGACCATGAT
AATAAACAAA AATCAGATGA CAGGGGTGAA TCAGAGCGAC ATCGAGGGGA TCAGTCTAGG
GTTCGAAGAC CAGAAACATT GAGATCCTCT AGTAGAAATG AACATGGCAT TAAATCTGAT
AGTTCAAAAA CTGATAAACT AGAACGAAAA CACAGGCATG AATCAGGGGA CTCAAGGGAA
AGACCATCTT CTGGGGAACA AAAATCAAGA CCTGACAGTC CTCGTGTTAA ACAAGGAGAT
TCTAATAAAT CAAGATCTGA TAAACTTGGT TTTAAATCAC CAACTAGTAA AGATGACAAA
AGGACAGAGG GTAACAAGAG TAAAGTAGAC ACTAATAAAG CACACCCTGA CAATAAGGCA
GAATTTCCAA GTTATTTGTT GGGGGGCAGG TCTGGTGCGT TGAAAAATTT TGTCATTCCG
AAAATCAAGA GGGATAAAGA TGGCAATGTT ACTCAGGAGA CAAAGAAAAT GGAAATGAAA
GGAGAGCCGA AAGACAAAGT AGAAAAAATA GGATTAGTTG AAGATCTAAA TAAAGGAGCT
AAGCCTGTAG TTGTGCTACA AAAACTGTCT TTGGATGATG TTCAGAAACT TATTAAAGAT
AGAGAGGACA AATCAAGAAG TTCCCTTAAA CCTATCAAGA ATAAACCATC AAAGTCAAAT
AAAGGTAGTA TAGATCAATC AGTGTTAAAA GAATTACCCC CTGAACTCCT GGCAGAAATT
GAGTCCACCA TGCCACTTTG TGAACGTGTG AAAATGAACA AACGCAAGCG TAGCACAGTT
AATGAAAAGC CAAAATATGC TGAAATCAGT TCAGATGAAG ATAATGATAG TGATGAAGCT
TTTGAATCCT CTAGGAAACG ACATAAAAAA GATGATGATA AAGCTTGGGA ATATGAAGAG
CGTGACAGAA GAAGCTCTGG GGATCATAGG AGAAGTGGCC ACTCTCATGA AGGAAGAAGG
AGTTCAGGTG GTGGTCGTTA TCGAAACCGA AGTCCGTCAG ATTCTGACAT GGAAGATTAT
TCTCCTCCTC CCAGCCTTAG TGAGGTTGCT AGGAAAATGA AGAAAAAAGA AAAACAGAAG
AAAAGGAAAG CATATGAACC AAAACTAACA CCTGAAGAAA TGATGGACTC TTCAACTTTT
AAGAGATTCA CAGCCTCAAT AGAGAATATT TTGGATAATT TGGAAGATAT GGATTTTACT
GCGTTTGGTG ATGATGATGA AATTCCTCAG GAACTGCTCT TAGGAAAACA TCAGCTTAAT
GAACTTGGCA GTGAATCTGC TAAAATAAAA GCAATGGGTA TAATGGATAA GCTTTCAACT
GACAAAACTG TGAAAGTCTT AAATATCTTG GAGAAGAATA TTCAGGATGG GTCAAAGCTT
TCCACTTTGT TAAATCATAA TAACGATACT GAAGAAGAAG AAAGGTTATG GAGAGACCTT
ATTATGGAGA GAGTTACAAA ATCAGCGGAT GCTTGTCTTA CAACTATCAA CATTATGACA
TCCCCTAACA TGCCAAAAGC TGTGTACATT GAGGATGTAA TTGAAAGAGT TATACAGTAC
ACTAAATTTC ATTTGCAGAA TACACTTTAT CCTCAGTATG ATCCTGTTTA CAGATTAGAT
CCTCATGGAG GAGGCTTATT AAGTTCAAAA GCAAAACGGG CTAAATGTTC TACCCATAAG
CAGAGAGTAA TAGTAATGCT TTATAACAAA GTTTGTGACA TTGTTAGCAG CTTATCAGAA
TTGCTAGAGA TACAACTTCT TACAGACACA ACAATTCTTC AGGTTTCATC TATGGGAATA
ACACCATTTT TTGTGGAAAA TGTCAGTGAA CTACAGTTGT GTGCCATTAA GTTAGTCACT
GCAGTATTCT CAAGATATGA AAAACATAGG CAGTTAATTT TGGAAGAAAT TTTTACTTCA
CTTGCAAGAT TACCAACCAG CAAGAGGAGT TTAAGGAACT TCAGGTTAAA CAGTAGTGAT
ATGGATGGAG AACCTATGTA TATTCAGATG GTTACAGCAC TGGTTTTACA ACTTATTCAG
TGTGTGGTAC ACTTACCATC ATCAGAGAAG GACTCTAATG CAGAAGAAGA TTCAAATAAA
AAAATTGACC AGGATGTTGT CATTACTAAC TCTTATGAAA CAGCTATGCG AACAGCCCAA
AACTTCCTCT CCATCTTCCT TAAAAAATGT GGTAGTAAGC AAGGTGAAGA AGATTACAGA
CCACTGTTTG AAAATTTTGT TCAAGACCTT CTTTCAACAG TCAATAAGCC TGAATGGCCA
GCTGCTGAAC TACTCCTTAG TTTGTTAGGG AGACTGTTGG TTCATCAGTT CAGTAACAAG
TCAACAGAGA TGGCTTTAAG AGTGGCATCT CTTGATTACC TTGGAACTGT TGCTGCACGG
CTAAGAAAAG ATGCTGTTAC AAGCAAAATG GATCAAGGAT CTATAGAACG CATTTTAAAA
CAGGTTTCAG GAGGGGAAGA TGAAATCCAA CAATTACAAA AAGCATTGCT TGATTACTTG
GATGAAAACA CTGAGACTGA TCCTTCACTA GTGTTTTCTC GTAAATTCTA TATAGCCCAG
TGGTTTCGAG ACACAACTCT GGAAACAGAA AAAGCAATGA AATCACAAAA AGATGAAGAA
TCATCTGAAG GAACACATCA TGCAAAGGAA ATTGAGACAA CTGGCCAAAT TATGCATCGA
GCTGAAAACC GAAAAAAGTT TCTTAGAAGC ATTATCAAAA CCACACCTTC TCAGTTTAGC
ACATTAAAGA TGAACTCTGA TACTGTGGAC TATGATGATG CTTGCTTGAT TGTTCGATAC
TTGGCCTCCA TGAGGCCGTT TGCCCAGAGC TTTGATATTT ATTTGACACA GATCCTACGA
GTTCTTGGTG AAAATGCAAT TGCTGTTCGA ACAAAAGCCA TGAAGTGTTT GTCTGAGGTT
GTTGCTGTAG ACCCCAGTAT TCTAGCAAGG CTTGATATGC AACGAGGTGT TCATGGACGA
TTGATGGATA ATTCGACTAG TGTCCGAGAA GCAGCAGTAG AATTACTAGG TCGATTTGTC
CTTTGTCGAC CTCAGCTTGC TGAACAGTAT TATGATATGC TGATTGAAAG AATATTGGAT
ACTGGTATCA GTGTCAGGAA AAGAGTAATA AAGATTCTCA GAGACATTTG TATTGAACAA
CCAACATTTC CAAAAATCAC AGAAATGTGT GTAAAAATGA TTCGCAGAGT CAATGATGAA
GAGGGCATTA AGAAATTAGT AAATGAAACA TTCCAGAAAC TCTGGTTTAC TCCAACTCCA
CACAATGACA AAGAAGCAAT GACAAGGAAA ATTTTAAACA TTACCGATGT GGTTGCAGCA
TGCAGAGATA CTGGATATGA CTGGTTTGAG CAACTGCTTC AAAACTTGTT GAAGTCCGAA
GAGGATTCCT CATATAAACC TGTGAAGAAA GCTTGTACTC AACTTGTTGA TAACCTAGTT
GAGCACATTC TTAAATATGA GGAATCTCTA GCTGACTCTG ACAATAAAGG TGTGAATTCT
GGAAGATTGG TAGCTTGCAT AACCACTTTG TTCTTATTCA GCAAAATAAG ACCCCAGCTC
ATGGTTAAAC ATGCAATGAC TATGCAACCA TACCTTACCA CTAAATGTAG TACGCAAAAT
GATTTCATGG TTATCTGCAA TGTTGCAAAA ATCCTAGAGC TAGTTGTACC ACTGATGGAG
CATCCAAGTG AAACTTTTCT TGCCACTATT GAGGAAGATC TAATGAAGCT CATCATCAAA
TATGGCATGA CTGTAGTGCA ACATTGTGTG AGCTGTCTTG GAGCTGTTGT AAATAAAGTG
ACACAAAATT TTAAATTTGT GTGGGCTTGT TTCAATAGAT ACTATGGTGC CATTTCAAAA
TTAAAAAGTC AACACCAAGA GGACCCAAAT AACACTTCAC TTCTAACAAA CAAACCAGCA
CTTCTTAGAT CCCTTTTCAC CGTTGGAGCA CTATGTCGGC ATTTTGATTT TGATCTGGAA
GATTTTAAAG GCAACAGCAA GGTTAACATA AAAGATAAAG TACTTGAACT ATTGATGTAT
TTTACAAAAC ACTCAGATGA AGAAGTACAA ACAAAAGCTA TCATTGGTCT AGGATTTGCC
TTTATTCAGC ATCCAAGTCT AATGTTCGAG CAAGAAGTGA AGAATCTATA TAATAATATT
TTATCTGATA AGAACTCCTC AGTCAATTTA AAAATACAAG TGTTAAAAAA CCTCCAGACC
TACCTACAAG AAGAAGATAC ACGTATGCAG CAGGCAGATA GAGACTGGAA GAAAGTTGCA
AAACAGGAAG ACTTAAAAGA AATGGGTGAT GTTTCCTCAG GGATGAGTAG TTCCATCATG
CAGCTTTATC TCAAACAGGT GCTTGAGGCA TTTTTTCACA CCCAGTCAAG TGTACGCCAC
TTTGCCCTAA ATGTCATTGC ATTGACTCTA AATCAAGGTC TTATTCATCC AGTTCAGTGT
GTGCCATATT TAATTGCTAT GGGCACAGAC CCAGAACCTG CTATGCGGAA CAAGGCTGAT
CAGCAACTTG TGGAAATAGA CAAAAAATAT GCTGGATTCA TTCATATGAA AGCAGTGGCT
GGTATGAAGA TGTCTTACCA GGTACAACAG GCAATCAACA CATGCCTAAA AGATCCTGTA
AGGGGTTTCA GACAAGACGA GTCCTCTAGC GCTTTGTGTT CACACCTTTA CTCCATGATC
CGTGGAAACC GCCAACACAG ACGAGCCTTT CTTATTTCTT TACTCAACCT CTTTGATGAC
ACAGCAAAAA CAGACGTGAC TATGCTCTTG TATATAGCAG ACAATCTAGC CTGTTTTCCA
TACCAGACAC AGGAAGAGCC GTTGTTTATA ATGCATCATA TAGACATTAC ACTCTCAGTT
TCTGGTAGTA ACCTACTGCA GTCATTCAAG GAGTCTATGG TAAAGGACAA AAGGAAAGAG
AGAAAATCAT CACCTAGTAA GGAAAATGAG TCAAGCGACA GTGAAGAAGA AGTTTCCAGG
CCTCGGAAGT CACGGAAACG TGTAGATTCA GATTCAGATT CAGATTCAGA AGACGATATA
AATTCAGTGA TGAAATGTTT GCCAGAAAAT TCAGCTCCTT TAATCGAATT TGCAAATGTG
TCCCAGGGTA TTTTATTACT TCTCATGTTA AAACAACATT TGAAGAATCT TTGTGGATTT
TCTGATAGTA AAATTCAGAA GTACTCTCCA TCTGAATCTG CAAAAGTATA TGATAAAGCG
ATAAACCGAA AAACAGGAGT TCATTTTCAT CCAAAACAAA CACTGGACTT CCTGCGGAGT
GACATGGCTA ATTCCAAAAT CACAGAAGAG GTGAAAAGGA GTATAGTAAA ACAGTATCTA
GATTTCAAAC TTCTCATGGA ACATCTGGAC CCTGATGAAG AAGAAGAAGA AGGGGAGGTT
TCAGCTAGCA CAAATGCTCG GAACAAAGCA ATTACCTCAC TGCTTGGAGG AGGCAGCCCT
AAAAATAATA CAGCAGCAGA GACAGAAGAT GATGAAAGTG ATGGGGAGGA TAGAGGAGGA
GGCACTTCAG GGTCATTGAG AAGGTCAAAA CGAAATTCAG ACTCTACGGA GTTGGCAGCA
CAGATGAATG AAAGTGTTGA CGTCATGGAT GTCATCGCTA TTTGCTGTCC AAAGTACAAA
GATCGACCAC AAATTGCAAG AGTAGTGCAG AAAACCAGCA GTGGCTTCAG TGTTCAGTGG
ATGGCAGGCT CCTACAGTGG CTCCTGGACT GAGGCTAAGC GCCGTGATGG CCGCAAACTG
GTGCCTTGGG TAGACACTAT TAAAGAGTCA GACATTATTT ACAAAAAAAT TGCTCTAACG
AGTGCTAATA AGCTGACTAA TAAAGTTGTT CAGACTTTAC GATCCCTGTA TGCCGCCAAG
GATGGGACTT CCAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016864819.1
CDS4380..11177
Misc Feature(1)6069..>6242
Misc Feature(2)8142..8267
Misc Feature(3)8193..8279
Misc Feature(4)8304..8384
Misc Feature(5)9588..10124
Translation

Target ORF information:

RefSeq Version XM_017009330.1
Organism Homo sapiens(Human)
Definition Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017009330.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
ATGGTTAGCA TTGATCTTCA TCAGGCAGGA AGAGTGGACT CTCAGGCTTC TATAACTCAG 
GATTCAGACT CCATAAAAAA GCCTGAAGAA ATCAAACAAT GTAATGATGC ACCTGTTTCT
GTTCTTCAGG AAGATATTGT TGGAAGTCTT AAATCTACAC CAGAAAACCA TCCTGAGACA
CCTAAAAAAA AGTCTGATCC TGAGCTTTCA AAGAGTGAAA TGAAACAAAG TGAAAGTAGA
TTAGCAGAAT CTAAACCAAA TGAAAACCGA TTGGTGGAGA CAAAATCAAG TGAAAATAAG
TTAGAAACTA AAGTTGAGAC CCAAACAGAA GAACTTAAAC AGAATGAGAG CAGAACAACT
GAATGCAAAC AAAACGAGAG CACCATAGTT GAGCCTAAAC AAAATGAAAA TAGACTGTCT
GACACAAAAC CAAATGACAA CAAACAAAAT AATGGCAGAT CAGAAACAAC AAAATCAAGG
CCTGAAACCC CAAAGCAAAA GGGTGAAAGC CGGCCTGAGA CTCCAAAACA AAAGAGTGAT
GGGCATCCTG AAACCCCAAA ACAGAAGGGT GATGGAAGGC CTGAAACTCC AAAGCAAAAA
GGTGAGAGCC GCCCTGAAAC TCCAAAGCAA AAAAATGAAG GGCGACCTGA AACACCAAAA
CACAGGCATG ACAATAGGAG GGATTCTGGA AAGCCATCTA CAGAGAAAAA ACCTGAAGTG
TCTAAACATA AACAAGATAC TAAATCTGAC TCACCTCGGT TAAAATCAGA ACGAGCTGAA
GCCTTAAAGC AGAGACCTGA TGGGCGATCT GTTTCTGAGT CACTAAGACG TGACCATGAT
AATAAACAAA AATCAGATGA CAGGGGTGAA TCAGAGCGAC ATCGAGGGGA TCAGTCTAGG
GTTCGAAGAC CAGAAACATT GAGATCCTCT AGTAGAAATG AACATGGCAT TAAATCTGAT
AGTTCAAAAA CTGATAAACT AGAACGAAAA CACAGGCATG AATCAGGGGA CTCAAGGGAA
AGACCATCTT CTGGGGAACA AAAATCAAGA CCTGACAGTC CTCGTGTTAA ACAAGGAGAT
TCTAATAAAT CAAGATCTGA TAAACTTGGT TTTAAATCAC CAACTAGTAA AGATGACAAA
AGGACAGAGG GTAACAAGAG TAAAGTAGAC ACTAATAAAG CACACCCTGA CAATAAGGCA
GAATTTCCAA GTTATTTGTT GGGGGGCAGG TCTGGTGCGT TGAAAAATTT TGTCATTCCG
AAAATCAAGA GGGATAAAGA TGGCAATGTT ACTCAGGAGA CAAAGAAAAT GGAAATGAAA
GGAGAGCCGA AAGACAAAGT AGAAAAAATA GGATTAGTTG AAGATCTAAA TAAAGGAGCT
AAGCCTGTAG TTGTGCTACA AAAACTGTCT TTGGATGATG TTCAGAAACT TATTAAAGAT
AGAGAGGACA AATCAAGAAG TTCCCTTAAA CCTATCAAGA ATAAACCATC AAAGTCAAAT
AAAGGTAGTA TAGATCAATC AGTGTTAAAA GAATTACCCC CTGAACTCCT GGCAGAAATT
GAGTCCACCA TGCCACTTTG TGAACGTGTG AAAATGAACA AACGCAAGCG TAGCACAGTT
AATGAAAAGC CAAAATATGC TGAAATCAGT TCAGATGAAG ATAATGATAG TGATGAAGCT
TTTGAATCCT CTAGGAAACG ACATAAAAAA GATGATGATA AAGCTTGGGA ATATGAAGAG
CGTGACAGAA GAAGCTCTGG GGATCATAGG AGAAGTGGCC ACTCTCATGA AGGAAGAAGG
AGTTCAGGTG GTGGTCGTTA TCGAAACCGA AGTCCGTCAG ATTCTGACAT GGAAGATTAT
TCTCCTCCTC CCAGCCTTAG TGAGGTTGCT AGGAAAATGA AGAAAAAAGA AAAACAGAAG
AAAAGGAAAG CATATGAACC AAAACTAACA CCTGAAGAAA TGATGGACTC TTCAACTTTT
AAGAGATTCA CAGCCTCAAT AGAGAATATT TTGGATAATT TGGAAGATAT GGATTTTACT
GCGTTTGGTG ATGATGATGA AATTCCTCAG GAACTGCTCT TAGGAAAACA TCAGCTTAAT
GAACTTGGCA GTGAATCTGC TAAAATAAAA GCAATGGGTA TAATGGATAA GCTTTCAACT
GACAAAACTG TGAAAGTCTT AAATATCTTG GAGAAGAATA TTCAGGATGG GTCAAAGCTT
TCCACTTTGT TAAATCATAA TAACGATACT GAAGAAGAAG AAAGGTTATG GAGAGACCTT
ATTATGGAGA GAGTTACAAA ATCAGCGGAT GCTTGTCTTA CAACTATCAA CATTATGACA
TCCCCTAACA TGCCAAAAGC TGTGTACATT GAGGATGTAA TTGAAAGAGT TATACAGTAC
ACTAAATTTC ATTTGCAGAA TACACTTTAT CCTCAGTATG ATCCTGTTTA CAGATTAGAT
CCTCATGGAG GAGGCTTATT AAGTTCAAAA GCAAAACGGG CTAAATGTTC TACCCATAAG
CAGAGAGTAA TAGTAATGCT TTATAACAAA GTTTGTGACA TTGTTAGCAG CTTATCAGAA
TTGCTAGAGA TACAACTTCT TACAGACACA ACAATTCTTC AGGTTTCATC TATGGGAATA
ACACCATTTT TTGTGGAAAA TGTCAGTGAA CTACAGTTGT GTGCCATTAA GTTAGTCACT
GCAGTATTCT CAAGATATGA AAAACATAGG CAGTTAATTT TGGAAGAAAT TTTTACTTCA
CTTGCAAGAT TACCAACCAG CAAGAGGAGT TTAAGGAACT TCAGGTTAAA CAGTAGTGAT
ATGGATGGAG AACCTATGTA TATTCAGATG GTTACAGCAC TGGTTTTACA ACTTATTCAG
TGTGTGGTAC ACTTACCATC ATCAGAGAAG GACTCTAATG CAGAAGAAGA TTCAAATAAA
AAAATTGACC AGGATGTTGT CATTACTAAC TCTTATGAAA CAGCTATGCG AACAGCCCAA
AACTTCCTCT CCATCTTCCT TAAAAAATGT GGTAGTAAGC AAGGTGAAGA AGATTACAGA
CCACTGTTTG AAAATTTTGT TCAAGACCTT CTTTCAACAG TCAATAAGCC TGAATGGCCA
GCTGCTGAAC TACTCCTTAG TTTGTTAGGG AGACTGTTGG TTCATCAGTT CAGTAACAAG
TCAACAGAGA TGGCTTTAAG AGTGGCATCT CTTGATTACC TTGGAACTGT TGCTGCACGG
CTAAGAAAAG ATGCTGTTAC AAGCAAAATG GATCAAGGAT CTATAGAACG CATTTTAAAA
CAGGTTTCAG GAGGGGAAGA TGAAATCCAA CAATTACAAA AAGCATTGCT TGATTACTTG
GATGAAAACA CTGAGACTGA TCCTTCACTA GTGTTTTCTC GTAAATTCTA TATAGCCCAG
TGGTTTCGAG ACACAACTCT GGAAACAGAA AAAGCAATGA AATCACAAAA AGATGAAGAA
TCATCTGAAG GAACACATCA TGCAAAGGAA ATTGAGACAA CTGGCCAAAT TATGCATCGA
GCTGAAAACC GAAAAAAGTT TCTTAGAAGC ATTATCAAAA CCACACCTTC TCAGTTTAGC
ACATTAAAGA TGAACTCTGA TACTGTGGAC TATGATGATG CTTGCTTGAT TGTTCGATAC
TTGGCCTCCA TGAGGCCGTT TGCCCAGAGC TTTGATATTT ATTTGACACA GATCCTACGA
GTTCTTGGTG AAAATGCAAT TGCTGTTCGA ACAAAAGCCA TGAAGTGTTT GTCTGAGGTT
GTTGCTGTAG ACCCCAGTAT TCTAGCAAGG CTTGATATGC AACGAGGTGT TCATGGACGA
TTGATGGATA ATTCGACTAG TGTCCGAGAA GCAGCAGTAG AATTACTAGG TCGATTTGTC
CTTTGTCGAC CTCAGCTTGC TGAACAGTAT TATGATATGC TGATTGAAAG AATATTGGAT
ACTGGTATCA GTGTCAGGAA AAGAGTAATA AAGATTCTCA GAGACATTTG TATTGAACAA
CCAACATTTC CAAAAATCAC AGAAATGTGT GTAAAAATGA TTCGCAGAGT CAATGATGAA
GAGGGCATTA AGAAATTAGT AAATGAAACA TTCCAGAAAC TCTGGTTTAC TCCAACTCCA
CACAATGACA AAGAAGCAAT GACAAGGAAA ATTTTAAACA TTACCGATGT GGTTGCAGCA
TGCAGAGATA CTGGATATGA CTGGTTTGAG CAACTGCTTC AAAACTTGTT GAAGTCCGAA
GAGGATTCCT CATATAAACC TGTGAAGAAA GCTTGTACTC AACTTGTTGA TAACCTAGTT
GAGCACATTC TTAAATATGA GGAATCTCTA GCTGACTCTG ACAATAAAGG TGTGAATTCT
GGAAGATTGG TAGCTTGCAT AACCACTTTG TTCTTATTCA GCAAAATAAG ACCCCAGCTC
ATGGTTAAAC ATGCAATGAC TATGCAACCA TACCTTACCA CTAAATGTAG TACGCAAAAT
GATTTCATGG TTATCTGCAA TGTTGCAAAA ATCCTAGAGC TAGTTGTACC ACTGATGGAG
CATCCAAGTG AAACTTTTCT TGCCACTATT GAGGAAGATC TAATGAAGCT CATCATCAAA
TATGGCATGA CTGTAGTGCA ACATTGTGTG AGCTGTCTTG GAGCTGTTGT AAATAAAGTG
ACACAAAATT TTAAATTTGT GTGGGCTTGT TTCAATAGAT ACTATGGTGC CATTTCAAAA
TTAAAAAGTC AACACCAAGA GGACCCAAAT AACACTTCAC TTCTAACAAA CAAACCAGCA
CTTCTTAGAT CCCTTTTCAC CGTTGGAGCA CTATGTCGGC ATTTTGATTT TGATCTGGAA
GATTTTAAAG GCAACAGCAA GGTTAACATA AAAGATAAAG TACTTGAACT ATTGATGTAT
TTTACAAAAC ACTCAGATGA AGAAGTACAA ACAAAAGCTA TCATTGGTCT AGGATTTGCC
TTTATTCAGC ATCCAAGTCT AATGTTCGAG CAAGAAGTGA AGAATCTATA TAATAATATT
TTATCTGATA AGAACTCCTC AGTCAATTTA AAAATACAAG TGTTAAAAAA CCTCCAGACC
TACCTACAAG AAGAAGATAC ACGTATGCAG CAGGCAGATA GAGACTGGAA GAAAGTTGCA
AAACAGGAAG ACTTAAAAGA AATGGGTGAT GTTTCCTCAG GGATGAGTAG TTCCATCATG
CAGCTTTATC TCAAACAGGT GCTTGAGGCA TTTTTTCACA CCCAGTCAAG TGTACGCCAC
TTTGCCCTAA ATGTCATTGC ATTGACTCTA AATCAAGGTC TTATTCATCC AGTTCAGTGT
GTGCCATATT TAATTGCTAT GGGCACAGAC CCAGAACCTG CTATGCGGAA CAAGGCTGAT
CAGCAACTTG TGGAAATAGA CAAAAAATAT GCTGGATTCA TTCATATGAA AGCAGTGGCT
GGTATGAAGA TGTCTTACCA GGTACAACAG GCAATCAACA CATGCCTAAA AGATCCTGTA
AGGGGTTTCA GACAAGACGA GTCCTCTAGC GCTTTGTGTT CACACCTTTA CTCCATGATC
CGTGGAAACC GCCAACACAG ACGAGCCTTT CTTATTTCTT TACTCAACCT CTTTGATGAC
ACAGCAAAAA CAGACGTGAC TATGCTCTTG TATATAGCAG ACAATCTAGC CTGTTTTCCA
TACCAGACAC AGGAAGAGCC GTTGTTTATA ATGCATCATA TAGACATTAC ACTCTCAGTT
TCTGGTAGTA ACCTACTGCA GTCATTCAAG GAGTCTATGG TAAAGGACAA AAGGAAAGAG
AGAAAATCAT CACCTAGTAA GGAAAATGAG TCAAGCGACA GTGAAGAAGA AGTTTCCAGG
CCTCGGAAGT CACGGAAACG TGTAGATTCA GATTCAGATT CAGATTCAGA AGACGATATA
AATTCAGTGA TGAAATGTTT GCCAGAAAAT TCAGCTCCTT TAATCGAATT TGCAAATGTG
TCCCAGGGTA TTTTATTACT TCTCATGTTA AAACAACATT TGAAGAATCT TTGTGGATTT
TCTGATAGTA AAATTCAGAA GTACTCTCCA TCTGAATCTG CAAAAGTATA TGATAAAGCG
ATAAACCGAA AAACAGGAGT TCATTTTCAT CCAAAACAAA CACTGGACTT CCTGCGGAGT
GACATGGCTA ATTCCAAAAT CACAGAAGAG GTGAAAAGGA GTATAGTAAA ACAGTATCTA
GATTTCAAAC TTCTCATGGA ACATCTGGAC CCTGATGAAG AAGAAGAAGA AGGGGAGGTT
TCAGCTAGCA CAAATGCTCG GAACAAAGCA ATTACCTCAC TGCTTGGAGG AGGCAGCCCT
AAAAATAATA CAGCAGCAGA GACAGAAGAT GATGAAAGTG ATGGGGAGGA TAGAGGAGGA
GGCACTTCAG GGTCATTGAG AAGGTCAAAA CGAAATTCAG ACTCTACGGA GTTGGCAGCA
CAGATGAATG AAAGTGTTGA CGTCATGGAT GTCATCGCTA TTTGCTGTCC AAAGTACAAA
GATCGACCAC AAATTGCAAG AGTAGTGCAG AAAACCAGCA GTGGCTTCAG TGTTCAGTGG
ATGGCAGGCT CCTACAGTGG CTCCTGGACT GAGGCTAAGC GCCGTGATGG CCGCAAACTG
GTGCCTTGGG TAGACACTAT TAAAGAGTCA GACATTATTT ACAAAAAAAT TGCTCTAACG
AGTGCTAATA AGCTGACTAA TAAAGTTGTT CAGACTTTAC GATCCCTGTA TGCCGCCAAG
GATGGGACTT CCAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu95812
Clone ID Related Accession (Same CDS sequence) XM_017009329.1
Accession Version XM_017009329.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 7950bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product nipped-B-like protein isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
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121
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481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA
GCATCTATGG TAAAGGACAA AAGGAAAGAG AGAAAATCAT CACCTAGTAA GGAAAATGAG
TCAAGCGACA GTGAAGAAGA AGTTTCCAGG CCTCGGAAGT CACGGAAACG TGTAGATTCA
GATTCAGATT CAGATTCAGA AGACGATATA AATTCAGTGA TGAAATGTTT GCCAGAAAAT
TCAGCTCCTT TAATCGAATT TGCAAATGTG TCCCAGGGTA TTTTATTACT TCTCATGTTA
AAACAACATT TGAAGAATCT TTGTGGATTT TCTGATAGTA AAATTCAGAA GTACTCTCCA
TCTGAATCTG CAAAAGTATA TGATAAAGCG ATAAACCGAA AAACAGGAGT TCATTTTCAT
CCAAAACAAA CACTGGACTT CCTGCGGAGT GACATGGCTA ATTCCAAAAT CACAGAAGAG
GTGAAAAGGA GTATAGTAAA ACAGTATCTA GATTTCAAAC TTCTCATGGA ACATCTGGAC
CCTGATGAAG AAGAAGAAGA AGGGGAGGTT TCAGCTAGCA CAAATGCTCG GAACAAAGCA
ATTACCTCAC TGCTTGGAGG AGGCAGCCCT AAAAATAATA CAGCAGCAGA GACAGAAGAT
GATGAAAGTG ATGGGGAGGA TAGAGGAGGA GGCACTTCAG GGGTGAGGCG GAGGAGGAGT
CAACGTATTT CGCAGCGTAT TACTCATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016864818.1
CDS489..8438
Translation

Target ORF information:

RefSeq Version XM_017009329.1
Organism Homo sapiens(Human)
Definition Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017009329.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA
GCATCTATGG TAAAGGACAA AAGGAAAGAG AGAAAATCAT CACCTAGTAA GGAAAATGAG
TCAAGCGACA GTGAAGAAGA AGTTTCCAGG CCTCGGAAGT CACGGAAACG TGTAGATTCA
GATTCAGATT CAGATTCAGA AGACGATATA AATTCAGTGA TGAAATGTTT GCCAGAAAAT
TCAGCTCCTT TAATCGAATT TGCAAATGTG TCCCAGGGTA TTTTATTACT TCTCATGTTA
AAACAACATT TGAAGAATCT TTGTGGATTT TCTGATAGTA AAATTCAGAA GTACTCTCCA
TCTGAATCTG CAAAAGTATA TGATAAAGCG ATAAACCGAA AAACAGGAGT TCATTTTCAT
CCAAAACAAA CACTGGACTT CCTGCGGAGT GACATGGCTA ATTCCAAAAT CACAGAAGAG
GTGAAAAGGA GTATAGTAAA ACAGTATCTA GATTTCAAAC TTCTCATGGA ACATCTGGAC
CCTGATGAAG AAGAAGAAGA AGGGGAGGTT TCAGCTAGCA CAAATGCTCG GAACAAAGCA
ATTACCTCAC TGCTTGGAGG AGGCAGCCCT AAAAATAATA CAGCAGCAGA GACAGAAGAT
GATGAAAGTG ATGGGGAGGA TAGAGGAGGA GGCACTTCAG GGGTGAGGCG GAGGAGGAGT
CAACGTATTT CGCAGCGTAT TACTCATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu95813
Clone ID Related Accession (Same CDS sequence) XM_005248282.4
Accession Version XM_005248282.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 7755bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product nipped-B-like protein isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005248282.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
ATGCTTAGGC TGTTGATTCT TTTGGATACA ATTTCAATTC TTCTAAGATA TACCAAGAAG 
AAAACAGGAA AGTGCAGAAG AATTATGCTT TTGAATTCCA ACACTTTACC TGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG
ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAT
CGACCACAAA TTGCAAGAGT AGTGCAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG
GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG
CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT
GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT
GGGACTTCCA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005248339.3
CDS651..8405
Translation

Target ORF information:

RefSeq Version XM_005248282.4
Organism Homo sapiens(Human)
Definition Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005248282.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
ATGCTTAGGC TGTTGATTCT TTTGGATACA ATTTCAATTC TTCTAAGATA TACCAAGAAG 
AAAACAGGAA AGTGCAGAAG AATTATGCTT TTGAATTCCA ACACTTTACC TGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG
ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAT
CGACCACAAA TTGCAAGAGT AGTGCAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG
GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG
CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT
GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT
GGGACTTCCA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu95815
Clone ID Related Accession (Same CDS sequence) XM_017009331.1
Accession Version XM_017009331.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6789bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product nipped-B-like protein isoform X8
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGGTAGT
ATAGATCAAT CAGTGTTAAA AGAATTACCC CCTGAACTCC TGGCAGAAAT TGAGTCCACC
ATGCCACTTT GTGAACGTGT GAAAATGAAC AAACGCAAGC GTAGCACAGT TAATGAAAAG
CCAAAATATG CTGAAATCAG TTCAGATGAA GATAATGATA GTGATGAAGC TTTTGAATCC
TCTAGGAAAC GACATAAAAA AGATGATGAT AAAGCTTGGG AATATGAAGA GCGTGACAGA
AGAAGCTCTG GGGATCATAG GAGAAGTGGC CACTCTCATG AAGGAAGAAG GAGTTCAGGT
GGTGGTCGTT ATCGAAACCG AAGTCCGTCA GATTCTGACA TGGAAGATTA TTCTCCTCCT
CCCAGCCTTA GTGAGGTTGC TAGGAAAATG AAGAAAAAAG AAAAACAGAA GAAAAGGAAA
GCATATGAAC CAAAACTAAC ACCTGAAGAA ATGATGGACT CTTCAACTTT TAAGAGATTC
ACAGCCTCAA TAGAGAATAT TTTGGATAAT TTGGAAGATA TGGATTTTAC TGCGTTTGGT
GATGATGATG AAATTCCTCA GGAACTGCTC TTAGGAAAAC ATCAGCTTAA TGAACTTGGC
AGTGAATCTG CTAAAATAAA AGCAATGGGT ATAATGGATA AGCTTTCAAC TGACAAAACT
GTGAAAGTCT TAAATATCTT GGAGAAGAAT ATTCAGGATG GGTCAAAGCT TTCCACTTTG
TTAAATCATA ATAACGATAC TGAAGAAGAA GAAAGGTTAT GGAGAGACCT TATTATGGAG
AGAGTTACAA AATCAGCGGA TGCTTGTCTT ACAACTATCA ACATTATGAC ATCCCCTAAC
ATGCCAAAAG CTGTGTACAT TGAGGATGTA ATTGAAAGAG TTATACAGTA CACTAAATTT
CATTTGCAGA ATACACTTTA TCCTCAGTAT GATCCTGTTT ACAGATTAGA TCCTCATGGA
GGAGGCTTAT TAAGTTCAAA AGCAAAACGG GCTAAATGTT CTACCCATAA GCAGAGAGTA
ATAGTAATGC TTTATAACAA AGTTTGTGAC ATTGTTAGCA GCTTATCAGA ATTGCTAGAG
ATACAACTTC TTACAGACAC AACAATTCTT CAGGTTTCAT CTATGGGAAT AACACCATTT
TTTGTGGAAA ATGTCAGTGA ACTACAGTTG TGTGCCATTA AGTTAGTCAC TGCAGTATTC
TCAAGATATG AAAAACATAG GCAGTTAATT TTGGAAGAAA TTTTTACTTC ACTTGCAAGA
TTACCAACCA GCAAGAGGAG TTTAAGGAAC TTCAGGTTAA ACAGTAGTGA TATGGATGGA
GAACCTATGT ATATTCAGAT GGTTACAGCA CTGGTTTTAC AACTTATTCA GTGTGTGGTA
CACTTACCAT CATCAGAGAA GGACTCTAAT GCAGAAGAAG ATTCAAATAA AAAAATTGAC
CAGGATGTTG TCATTACTAA CTCTTATGAA ACAGCTATGC GAACAGCCCA AAACTTCCTC
TCCATCTTCC TTAAAAAATG TGGTAGTAAG CAAGGTGAAG AAGATTACAG ACCACTGTTT
GAAAATTTTG TTCAAGACCT TCTTTCAACA GTCAATAAGC CTGAATGGCC AGCTGCTGAA
CTACTCCTTA GTTTGTTAGG GAGACTGTTG GTTCATCAGT TCAGTAACAA GTCAACAGAG
ATGGCTTTAA GAGTGGCATC TCTTGATTAC CTTGGAACTG TTGCTGCACG GCTAAGAAAA
GATGCTGTTA CAAGCAAAAT GGATCAAGGA TCTATAGAAC GCATTTTAAA ACAGGTTTCA
GGAGGGGAAG ATGAAATCCA ACAATTACAA AAAGCATTGC TTGATTACTT GGATGAAAAC
ACTGAGACTG ATCCTTCACT AGTGTTTTCT CGTAAATTCT ATATAGCCCA GTGGTTTCGA
GACACAACTC TGGAAACAGA AAAAGCAATG AAATCACAAA AAGATGAAGA ATCATCTGAA
GGAACACATC ATGCAAAGGA AATTGAGACA ACTGGCCAAA TTATGCATCG AGCTGAAAAC
CGAAAAAAGT TTCTTAGAAG CATTATCAAA ACCACACCTT CTCAGTTTAG CACATTAAAG
ATGAACTCTG ATACTGTGGA CTATGATGAT GCTTGCTTGA TTGTTCGATA CTTGGCCTCC
ATGAGGCCGT TTGCCCAGAG CTTTGATATT TATTTGACAC AGATCCTACG AGTTCTTGGT
GAAAATGCAA TTGCTGTTCG AACAAAAGCC ATGAAGTGTT TGTCTGAGGT TGTTGCTGTA
GACCCCAGTA TTCTAGCAAG GCTTGATATG CAACGAGGTG TTCATGGACG ATTGATGGAT
AATTCGACTA GTGTCCGAGA AGCAGCAGTA GAATTACTAG GTCGATTTGT CCTTTGTCGA
CCTCAGCTTG CTGAACAGTA TTATGATATG CTGATTGAAA GAATATTGGA TACTGGTATC
AGTGTCAGGA AAAGAGTAAT AAAGATTCTC AGAGACATTT GTATTGAACA ACCAACATTT
CCAAAAATCA CAGAAATGTG TGTAAAAATG ATTCGCAGAG TCAATGATGA AGAGGGCATT
AAGAAATTAG TAAATGAAAC ATTCCAGAAA CTCTGGTTTA CTCCAACTCC ACACAATGAC
AAAGAAGCAA TGACAAGGAA AATTTTAAAC ATTACCGATG TGGTTGCAGC ATGCAGAGAT
ACTGGATATG ACTGGTTTGA GCAACTGCTT CAAAACTTGT TGAAGTCCGA AGAGGATTCC
TCATATAAAC CTGTGAAGAA AGCTTGTACT CAACTTGTTG ATAACCTAGT TGAGCACATT
CTTAAATATG AGGAATCTCT AGCTGACTCT GACAATAAAG GTGTGAATTC TGGAAGATTG
GTAGCTTGCA TAACCACTTT GTTCTTATTC AGCAAAATAA GACCCCAGCT CATGGTTAAA
CATGCAATGA CTATGCAACC ATACCTTACC ACTAAATGTA GTACGCAAAA TGATTTCATG
GTTATCTGCA ATGTTGCAAA AATCCTAGAG CTAGTTGTAC CACTGATGGA GCATCCAAGT
GAAACTTTTC TTGCCACTAT TGAGGAAGAT CTAATGAAGC TCATCATCAA ATATGGCATG
ACTGTAGTGC AACATTGTGT GAGCTGTCTT GGAGCTGTTG TAAATAAAGT GACACAAAAT
TTTAAATTTG TGTGGGCTTG TTTCAATAGA TACTATGGTG CCATTTCAAA ATTAAAAAGT
CAACACCAAG AGGACCCAAA TAACACTTCA CTTCTAACAA ACAAACCAGC ACTTCTTAGA
TCCCTTTTCA CCGTTGGAGC ACTATGTCGG CATTTTGATT TTGATCTGGA AGATTTTAAA
GGCAACAGCA AGGTTAACAT AAAAGATAAA GTACTTGAAC TATTGATGTA TTTTACAAAA
CACTCAGATG AAGAAGTACA AACAAAAGCT ATCATTGGTC TAGGATTTGC CTTTATTCAG
CATCCAAGTC TAATGTTCGA GCAAGAAGTG AAGAATCTAT ATAATAATAT TTTATCTGAT
AAGAACTCCT CAGTCAATTT AAAAATACAA GTGTTAAAAA ACCTCCAGAC CTACCTACAA
GAAGAAGATA CACGTATGCA GCAGGCAGAT AGAGACTGGA AGAAAGTTGC AAAACAGGAA
GACTTAAAAG AAATGGGTGA TGTTTCCTCA GGGATGAGTA GTTCCATCAT GCAGCTTTAT
CTCAAACAGG TGCTTGAGGC ATTTTTTCAC ACCCAGTCAA GTGTACGCCA CTTTGCCCTA
AATGTCATTG CATTGACTCT AAATCAAGGT CTTATTCATC CAGTTCAGTG TGTGCCATAT
TTAATTGCTA TGGGCACAGA CCCAGAACCT GCTATGCGGA ACAAGGCTGA TCAGCAACTT
GTGGAAATAG ACAAAAAATA TGCTGGATTC ATTCATATGA AAGCAGTGGC TGGTATGAAG
ATGTCTTACC AGGTACAACA GGCAATCAAC ACATGCCTAA AAGATCCTGT AAGGGGTTTC
AGACAAGACG AGTCCTCTAG CGCTTTGTGT TCACACCTTT ACTCCATGAT CCGTGGAAAC
CGCCAACACA GACGAGCCTT TCTTATTTCT TTACTCAACC TCTTTGATGA CACAGCAAAA
ACAGACGTGA CTATGCTCTT GTATATAGCA GACAATCTAG CCTGTTTTCC ATACCAGACA
CAGGAAGAGC CGTTGTTTAT AATGCATCAT ATAGACATTA CACTCTCAGT TTCTGGTAGT
AACCTACTGC AGTCATTCAA GGAGTCTATG GTAAAGGACA AAAGGAAAGA GAGAAAATCA
TCACCTAGTA AGGAAAATGA GTCAAGCGAC AGTGAAGAAG AAGTTTCCAG GCCTCGGAAG
TCACGGAAAC GTGTAGATTC AGATTCAGAT TCAGATTCAG AAGACGATAT AAATTCAGTG
ATGAAATGTT TGCCAGAAAA TTCAGCTCCT TTAATCGAAT TTGCAAATGT GTCCCAGGGT
ATTTTATTAC TTCTCATGTT AAAACAACAT TTGAAGAATC TTTGTGGATT TTCTGATAGT
AAAATTCAGA AGTACTCTCC ATCTGAATCT GCAAAAGTAT ATGATAAAGC GATAAACCGA
AAAACAGGAG TTCATTTTCA TCCAAAACAA ACACTGGACT TCCTGCGGAG TGACATGGCT
AATTCCAAAA TCACAGAAGA GGTGAAAAGG AGTATAGTAA AACAGTATCT AGATTTCAAA
CTTCTCATGG AACATCTGGA CCCTGATGAA GAAGAAGAAG AAGGGGAGGT TTCAGCTAGC
ACAAATGCTC GGAACAAAGC AATTACCTCA CTGCTTGGAG GAGGCAGCCC TAAAAATAAT
ACAGCAGCAG AGACAGAAGA TGATGAAAGT GATGGGGAGG ATAGAGGAGG AGGCACTTCA
GGGTCATTGA GAAGGTCAAA ACGAAATTCA GACTCTACGG AGTTGGCAGC ACAGATGAAT
GAAAGTGTTG ACGTCATGGA TGTCATCGCT ATTTGCTGTC CAAAGTACAA AGATCGACCA
CAAATTGCAA GAGTAGTGCA GAAAACCAGC AGTGGCTTCA GTGTTCAGTG GATGGCAGGC
TCCTACAGTG GCTCCTGGAC TGAGGCTAAG CGCCGTGATG GCCGCAAACT GGTGCCTTGG
GTAGACACTA TTAAAGAGTC AGACATTATT TACAAAAAAA TTGCTCTAAC GAGTGCTAAT
AAGCTGACTA ATAAAGTTGT TCAGACTTTA CGATCCCTGT ATGCCGCCAA GGATGGGACT
TCCAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016864820.1
CDS489..7277
Translation

Target ORF information:

RefSeq Version XM_017009331.1
Organism Homo sapiens(Human)
Definition Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017009331.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGGTAGT
ATAGATCAAT CAGTGTTAAA AGAATTACCC CCTGAACTCC TGGCAGAAAT TGAGTCCACC
ATGCCACTTT GTGAACGTGT GAAAATGAAC AAACGCAAGC GTAGCACAGT TAATGAAAAG
CCAAAATATG CTGAAATCAG TTCAGATGAA GATAATGATA GTGATGAAGC TTTTGAATCC
TCTAGGAAAC GACATAAAAA AGATGATGAT AAAGCTTGGG AATATGAAGA GCGTGACAGA
AGAAGCTCTG GGGATCATAG GAGAAGTGGC CACTCTCATG AAGGAAGAAG GAGTTCAGGT
GGTGGTCGTT ATCGAAACCG AAGTCCGTCA GATTCTGACA TGGAAGATTA TTCTCCTCCT
CCCAGCCTTA GTGAGGTTGC TAGGAAAATG AAGAAAAAAG AAAAACAGAA GAAAAGGAAA
GCATATGAAC CAAAACTAAC ACCTGAAGAA ATGATGGACT CTTCAACTTT TAAGAGATTC
ACAGCCTCAA TAGAGAATAT TTTGGATAAT TTGGAAGATA TGGATTTTAC TGCGTTTGGT
GATGATGATG AAATTCCTCA GGAACTGCTC TTAGGAAAAC ATCAGCTTAA TGAACTTGGC
AGTGAATCTG CTAAAATAAA AGCAATGGGT ATAATGGATA AGCTTTCAAC TGACAAAACT
GTGAAAGTCT TAAATATCTT GGAGAAGAAT ATTCAGGATG GGTCAAAGCT TTCCACTTTG
TTAAATCATA ATAACGATAC TGAAGAAGAA GAAAGGTTAT GGAGAGACCT TATTATGGAG
AGAGTTACAA AATCAGCGGA TGCTTGTCTT ACAACTATCA ACATTATGAC ATCCCCTAAC
ATGCCAAAAG CTGTGTACAT TGAGGATGTA ATTGAAAGAG TTATACAGTA CACTAAATTT
CATTTGCAGA ATACACTTTA TCCTCAGTAT GATCCTGTTT ACAGATTAGA TCCTCATGGA
GGAGGCTTAT TAAGTTCAAA AGCAAAACGG GCTAAATGTT CTACCCATAA GCAGAGAGTA
ATAGTAATGC TTTATAACAA AGTTTGTGAC ATTGTTAGCA GCTTATCAGA ATTGCTAGAG
ATACAACTTC TTACAGACAC AACAATTCTT CAGGTTTCAT CTATGGGAAT AACACCATTT
TTTGTGGAAA ATGTCAGTGA ACTACAGTTG TGTGCCATTA AGTTAGTCAC TGCAGTATTC
TCAAGATATG AAAAACATAG GCAGTTAATT TTGGAAGAAA TTTTTACTTC ACTTGCAAGA
TTACCAACCA GCAAGAGGAG TTTAAGGAAC TTCAGGTTAA ACAGTAGTGA TATGGATGGA
GAACCTATGT ATATTCAGAT GGTTACAGCA CTGGTTTTAC AACTTATTCA GTGTGTGGTA
CACTTACCAT CATCAGAGAA GGACTCTAAT GCAGAAGAAG ATTCAAATAA AAAAATTGAC
CAGGATGTTG TCATTACTAA CTCTTATGAA ACAGCTATGC GAACAGCCCA AAACTTCCTC
TCCATCTTCC TTAAAAAATG TGGTAGTAAG CAAGGTGAAG AAGATTACAG ACCACTGTTT
GAAAATTTTG TTCAAGACCT TCTTTCAACA GTCAATAAGC CTGAATGGCC AGCTGCTGAA
CTACTCCTTA GTTTGTTAGG GAGACTGTTG GTTCATCAGT TCAGTAACAA GTCAACAGAG
ATGGCTTTAA GAGTGGCATC TCTTGATTAC CTTGGAACTG TTGCTGCACG GCTAAGAAAA
GATGCTGTTA CAAGCAAAAT GGATCAAGGA TCTATAGAAC GCATTTTAAA ACAGGTTTCA
GGAGGGGAAG ATGAAATCCA ACAATTACAA AAAGCATTGC TTGATTACTT GGATGAAAAC
ACTGAGACTG ATCCTTCACT AGTGTTTTCT CGTAAATTCT ATATAGCCCA GTGGTTTCGA
GACACAACTC TGGAAACAGA AAAAGCAATG AAATCACAAA AAGATGAAGA ATCATCTGAA
GGAACACATC ATGCAAAGGA AATTGAGACA ACTGGCCAAA TTATGCATCG AGCTGAAAAC
CGAAAAAAGT TTCTTAGAAG CATTATCAAA ACCACACCTT CTCAGTTTAG CACATTAAAG
ATGAACTCTG ATACTGTGGA CTATGATGAT GCTTGCTTGA TTGTTCGATA CTTGGCCTCC
ATGAGGCCGT TTGCCCAGAG CTTTGATATT TATTTGACAC AGATCCTACG AGTTCTTGGT
GAAAATGCAA TTGCTGTTCG AACAAAAGCC ATGAAGTGTT TGTCTGAGGT TGTTGCTGTA
GACCCCAGTA TTCTAGCAAG GCTTGATATG CAACGAGGTG TTCATGGACG ATTGATGGAT
AATTCGACTA GTGTCCGAGA AGCAGCAGTA GAATTACTAG GTCGATTTGT CCTTTGTCGA
CCTCAGCTTG CTGAACAGTA TTATGATATG CTGATTGAAA GAATATTGGA TACTGGTATC
AGTGTCAGGA AAAGAGTAAT AAAGATTCTC AGAGACATTT GTATTGAACA ACCAACATTT
CCAAAAATCA CAGAAATGTG TGTAAAAATG ATTCGCAGAG TCAATGATGA AGAGGGCATT
AAGAAATTAG TAAATGAAAC ATTCCAGAAA CTCTGGTTTA CTCCAACTCC ACACAATGAC
AAAGAAGCAA TGACAAGGAA AATTTTAAAC ATTACCGATG TGGTTGCAGC ATGCAGAGAT
ACTGGATATG ACTGGTTTGA GCAACTGCTT CAAAACTTGT TGAAGTCCGA AGAGGATTCC
TCATATAAAC CTGTGAAGAA AGCTTGTACT CAACTTGTTG ATAACCTAGT TGAGCACATT
CTTAAATATG AGGAATCTCT AGCTGACTCT GACAATAAAG GTGTGAATTC TGGAAGATTG
GTAGCTTGCA TAACCACTTT GTTCTTATTC AGCAAAATAA GACCCCAGCT CATGGTTAAA
CATGCAATGA CTATGCAACC ATACCTTACC ACTAAATGTA GTACGCAAAA TGATTTCATG
GTTATCTGCA ATGTTGCAAA AATCCTAGAG CTAGTTGTAC CACTGATGGA GCATCCAAGT
GAAACTTTTC TTGCCACTAT TGAGGAAGAT CTAATGAAGC TCATCATCAA ATATGGCATG
ACTGTAGTGC AACATTGTGT GAGCTGTCTT GGAGCTGTTG TAAATAAAGT GACACAAAAT
TTTAAATTTG TGTGGGCTTG TTTCAATAGA TACTATGGTG CCATTTCAAA ATTAAAAAGT
CAACACCAAG AGGACCCAAA TAACACTTCA CTTCTAACAA ACAAACCAGC ACTTCTTAGA
TCCCTTTTCA CCGTTGGAGC ACTATGTCGG CATTTTGATT TTGATCTGGA AGATTTTAAA
GGCAACAGCA AGGTTAACAT AAAAGATAAA GTACTTGAAC TATTGATGTA TTTTACAAAA
CACTCAGATG AAGAAGTACA AACAAAAGCT ATCATTGGTC TAGGATTTGC CTTTATTCAG
CATCCAAGTC TAATGTTCGA GCAAGAAGTG AAGAATCTAT ATAATAATAT TTTATCTGAT
AAGAACTCCT CAGTCAATTT AAAAATACAA GTGTTAAAAA ACCTCCAGAC CTACCTACAA
GAAGAAGATA CACGTATGCA GCAGGCAGAT AGAGACTGGA AGAAAGTTGC AAAACAGGAA
GACTTAAAAG AAATGGGTGA TGTTTCCTCA GGGATGAGTA GTTCCATCAT GCAGCTTTAT
CTCAAACAGG TGCTTGAGGC ATTTTTTCAC ACCCAGTCAA GTGTACGCCA CTTTGCCCTA
AATGTCATTG CATTGACTCT AAATCAAGGT CTTATTCATC CAGTTCAGTG TGTGCCATAT
TTAATTGCTA TGGGCACAGA CCCAGAACCT GCTATGCGGA ACAAGGCTGA TCAGCAACTT
GTGGAAATAG ACAAAAAATA TGCTGGATTC ATTCATATGA AAGCAGTGGC TGGTATGAAG
ATGTCTTACC AGGTACAACA GGCAATCAAC ACATGCCTAA AAGATCCTGT AAGGGGTTTC
AGACAAGACG AGTCCTCTAG CGCTTTGTGT TCACACCTTT ACTCCATGAT CCGTGGAAAC
CGCCAACACA GACGAGCCTT TCTTATTTCT TTACTCAACC TCTTTGATGA CACAGCAAAA
ACAGACGTGA CTATGCTCTT GTATATAGCA GACAATCTAG CCTGTTTTCC ATACCAGACA
CAGGAAGAGC CGTTGTTTAT AATGCATCAT ATAGACATTA CACTCTCAGT TTCTGGTAGT
AACCTACTGC AGTCATTCAA GGAGTCTATG GTAAAGGACA AAAGGAAAGA GAGAAAATCA
TCACCTAGTA AGGAAAATGA GTCAAGCGAC AGTGAAGAAG AAGTTTCCAG GCCTCGGAAG
TCACGGAAAC GTGTAGATTC AGATTCAGAT TCAGATTCAG AAGACGATAT AAATTCAGTG
ATGAAATGTT TGCCAGAAAA TTCAGCTCCT TTAATCGAAT TTGCAAATGT GTCCCAGGGT
ATTTTATTAC TTCTCATGTT AAAACAACAT TTGAAGAATC TTTGTGGATT TTCTGATAGT
AAAATTCAGA AGTACTCTCC ATCTGAATCT GCAAAAGTAT ATGATAAAGC GATAAACCGA
AAAACAGGAG TTCATTTTCA TCCAAAACAA ACACTGGACT TCCTGCGGAG TGACATGGCT
AATTCCAAAA TCACAGAAGA GGTGAAAAGG AGTATAGTAA AACAGTATCT AGATTTCAAA
CTTCTCATGG AACATCTGGA CCCTGATGAA GAAGAAGAAG AAGGGGAGGT TTCAGCTAGC
ACAAATGCTC GGAACAAAGC AATTACCTCA CTGCTTGGAG GAGGCAGCCC TAAAAATAAT
ACAGCAGCAG AGACAGAAGA TGATGAAAGT GATGGGGAGG ATAGAGGAGG AGGCACTTCA
GGGTCATTGA GAAGGTCAAA ACGAAATTCA GACTCTACGG AGTTGGCAGC ACAGATGAAT
GAAAGTGTTG ACGTCATGGA TGTCATCGCT ATTTGCTGTC CAAAGTACAA AGATCGACCA
CAAATTGCAA GAGTAGTGCA GAAAACCAGC AGTGGCTTCA GTGTTCAGTG GATGGCAGGC
TCCTACAGTG GCTCCTGGAC TGAGGCTAAG CGCCGTGATG GCCGCAAACT GGTGCCTTGG
GTAGACACTA TTAAAGAGTC AGACATTATT TACAAAAAAA TTGCTCTAAC GAGTGCTAAT
AAGCTGACTA ATAAAGTTGT TCAGACTTTA CGATCCCTGT ATGCCGCCAA GGATGGGACT
TCCAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26171
Clone ID Related Accession (Same CDS sequence) NM_133433.3
Accession Version NM_133433.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8415bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product nipped-B-like protein isoform A
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN369580.1 and AJ627032.1. This sequence is a reference standard in the RefSeqGene project. On May 29, 2008 this sequence version replaced NM_133433.2. Transcript Variant: This variant (A) encodes the longer isoform (A). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ627032.1, BK005151.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG
ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAT
CGACCACAAA TTGCAAGAGT AGTGCAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG
GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG
CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT
GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT
GGGACTTCCA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_597677.2
CDS500..8914
Misc Feature(1)473..475
Misc Feature(2)<836..1420
Misc Feature(3)947..949
Misc Feature(4)983..985
Misc Feature(5)1226..1228
Misc Feature(6)1265..1267
Misc Feature(7)1319..1321
Misc Feature(8)1337..1339
Misc Feature(9)1349..1351
Misc Feature(10)1400..1402
Misc Feature(11)1412..1414
Misc Feature(12)1415..1417
Misc Feature(13)1451..1453
Misc Feature(14)1451..1453
Misc Feature(15)1547..1549
Misc Feature(16)1547..1549
Misc Feature(17)1631..1633
Misc Feature(18)2435..2437
Misc Feature(19)2636..2638
Misc Feature(20)2636..2638
Misc Feature(21)2735..2737
Misc Feature(22)3044..3046
Misc Feature(23)3047..3049
Misc Feature(24)3119..3121
Misc Feature(25)3128..3130
Misc Feature(26)3233..3235
Misc Feature(27)3485..3526
Misc Feature(28)3743..3745
Misc Feature(29)3752..3754
Misc Feature(30)3764..3766
Misc Feature(31)3764..3766
Misc Feature(32)3767..3769
Misc Feature(33)3767..3769
Misc Feature(34)3785..3787
Misc Feature(35)3785..3787
Misc Feature(36)3806..>3979
Misc Feature(37)3947..3949
Misc Feature(38)3953..3955
Misc Feature(39)3959..3961
Misc Feature(40)3974..3976
Misc Feature(41)3977..3979
Misc Feature(42)4064..4066
Misc Feature(43)4088..4090
Misc Feature(44)5798..5914
Misc Feature(45)5879..6004
Misc Feature(46)5930..6016
Misc Feature(47)6026..6142
Misc Feature(48)6041..6121
Misc Feature(49)6332..6451
Misc Feature(50)7178..7300
Misc Feature(51)7325..7861
Misc Feature(52)7436..7552
Misc Feature(53)7976..7978
Misc Feature(54)8024..8026
Misc Feature(55)8030..8032
Misc Feature(56)8036..8038
Misc Feature(57)8042..8044
Misc Feature(58)8453..8455
Misc Feature(59)8471..8473
Misc Feature(60)8471..8473
Misc Feature(61)8498..8500
Misc Feature(62)8513..8515
Exon (1)1..420
Exon (2)421..563
Exon (3)564..729
Exon (4)730..857
Exon (5)858..957
Exon (6)958..1109
Exon (7)1110..1270
Exon (8)1271..1367
Exon (9)1368..1994
Exon (10)1995..3620
Exon (11)3621..3803
Exon (12)3804..4001
Exon (13)4002..4073
Exon (14)4074..4163
Exon (15)4164..4267
Exon (16)4268..4354
Exon (17)4355..4586
Exon (18)4587..4738
Exon (19)4739..4819
Exon (20)4820..4920
Exon (21)4921..5059
Exon (22)5060..5142
Exon (23)5143..5275
Exon (24)5276..5419
Exon (25)5420..5509
Exon (26)5510..5724
Exon (27)5725..5827
Exon (28)5828..5926
Exon (29)5927..6073
Exon (30)6074..6208
Exon (31)6209..6307
Exon (32)6308..6361
Exon (33)6362..6470
Exon (34)6471..6607
Exon (35)6608..6748
Exon (36)6749..6842
Exon (37)6843..6997
Exon (38)6998..7088
Exon (39)7089..7262
Exon (40)7263..7453
Exon (41)7454..7561
Exon (42)7562..7762
Exon (43)7763..7909
Exon (44)7910..8184
Exon (45)8185..8359
Exon (46)8360..8548
Exon (47)8549..9841
Translation

Target ORF information:

RefSeq Version NM_133433.3
Organism Homo sapiens(Human)
Definition Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant A, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_133433.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG
ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAT
CGACCACAAA TTGCAAGAGT AGTGCAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG
GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG
CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT
GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT
GGGACTTCCA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26399
Clone ID Related Accession (Same CDS sequence) NM_015384.4
Accession Version NM_015384.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8094bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product nipped-B-like protein isoform B
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN369580.1, AJ640137.1, AA508213.1, BQ723324.1 and AJ627032.1. This sequence is a reference standard in the RefSeqGene project. On May 29, 2008 this sequence version replaced NM_015384.3. Transcript Variant: This variant (B) is alternatively spliced at the 3' end compared to transcript variant A. It encodes an isoform (B) with a shorter, distinct C-terminus, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ640137.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_056199.2
CDS500..8593
Misc Feature(1)473..475
Misc Feature(2)<836..1420
Misc Feature(3)947..949
Misc Feature(4)983..985
Misc Feature(5)1226..1228
Misc Feature(6)1265..1267
Misc Feature(7)1319..1321
Misc Feature(8)1337..1339
Misc Feature(9)1349..1351
Misc Feature(10)1400..1402
Misc Feature(11)1412..1414
Misc Feature(12)1415..1417
Misc Feature(13)1451..1453
Misc Feature(14)1451..1453
Misc Feature(15)1547..1549
Misc Feature(16)1547..1549
Misc Feature(17)1631..1633
Misc Feature(18)2435..2437
Misc Feature(19)2636..2638
Misc Feature(20)2636..2638
Misc Feature(21)2735..2737
Misc Feature(22)3044..3046
Misc Feature(23)3047..3049
Misc Feature(24)3119..3121
Misc Feature(25)3128..3130
Misc Feature(26)3233..3235
Misc Feature(27)3485..3526
Misc Feature(28)3743..3745
Misc Feature(29)3752..3754
Misc Feature(30)3764..3766
Misc Feature(31)3764..3766
Misc Feature(32)3767..3769
Misc Feature(33)3767..3769
Misc Feature(34)3785..3787
Misc Feature(35)3785..3787
Misc Feature(36)3806..>3979
Misc Feature(37)3947..3949
Misc Feature(38)3953..3955
Misc Feature(39)3959..3961
Misc Feature(40)3974..3976
Misc Feature(41)3977..3979
Misc Feature(42)4064..4066
Misc Feature(43)4088..4090
Misc Feature(44)5798..5914
Misc Feature(45)5879..6004
Misc Feature(46)5930..6016
Misc Feature(47)6026..6142
Misc Feature(48)6041..6121
Misc Feature(49)6332..6451
Misc Feature(50)7178..7300
Misc Feature(51)7325..7861
Misc Feature(52)7436..7552
Misc Feature(53)7976..7978
Misc Feature(54)8024..8026
Misc Feature(55)8030..8032
Misc Feature(56)8036..8038
Misc Feature(57)8042..8044
Misc Feature(58)8453..8455
Misc Feature(59)8471..8473
Misc Feature(60)8471..8473
Misc Feature(61)8498..8500
Misc Feature(62)8513..8515
Exon (1)1..420
Exon (2)421..563
Exon (3)564..729
Exon (4)730..857
Exon (5)858..957
Exon (6)958..1109
Exon (7)1110..1270
Exon (8)1271..1367
Exon (9)1368..1994
Exon (10)1995..3620
Exon (11)3621..3803
Exon (12)3804..4001
Exon (13)4002..4073
Exon (14)4074..4163
Exon (15)4164..4267
Exon (16)4268..4354
Exon (17)4355..4586
Exon (18)4587..4738
Exon (19)4739..4819
Exon (20)4820..4920
Exon (21)4921..5059
Exon (22)5060..5142
Exon (23)5143..5275
Exon (24)5276..5419
Exon (25)5420..5509
Exon (26)5510..5724
Exon (27)5725..5827
Exon (28)5828..5926
Exon (29)5927..6073
Exon (30)6074..6208
Exon (31)6209..6307
Exon (32)6308..6361
Exon (33)6362..6470
Exon (34)6471..6607
Exon (35)6608..6748
Exon (36)6749..6842
Exon (37)6843..6997
Exon (38)6998..7088
Exon (39)7089..7262
Exon (40)7263..7453
Exon (41)7454..7561
Exon (42)7562..7762
Exon (43)7763..7909
Exon (44)7910..8184
Exon (45)8185..8359
Exon (46)8360..10389
Translation

Target ORF information:

RefSeq Version NM_015384.4
Organism Homo sapiens(Human)
Definition Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant B, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_015384.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu39306
Clone ID Related Accession (Same CDS sequence) XM_006714467.2
Accession Version XM_006714467.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8268bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product nipped-B-like protein isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006714467.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA
GCATCTATGG TAAAGGACAA AAGGAAAGAG AGAAAATCAT CACCTAGTAA GGAAAATGAG
TCAAGCGACA GTGAAGAAGA AGTTTCCAGG CCTCGGAAGT CACGGAAACG TGTAGATTCA
GATTCAGATT CAGATTCAGA AGACGATATA AATTCAGTGA TGAAATGTTT GCCAGAAAAT
TCAGCTCCTT TAATCGAATT TGCAAATGTG TCCCAGGGTA TTTTATTACT TCTCATGTTA
AAACAACATT TGAAGAATCT TTGTGGATTT TCTGATAGTA AAATTCAGAA GTACTCTCCA
TCTGAATCTG CAAAAGTATA TGATAAAGCG ATAAACCGAA AAACAGGAGT TCATTTTCAT
CCAAAACAAA CACTGGACTT CCTGCGGAGT GACATGGCTA ATTCCAAAAT CACAGAAGAG
GTGAAAAGGA GTATAGTAAA ACAGTATCTA GATTTCAAAC TTCTCATGGA ACATCTGGAC
CCTGATGAAG AAGAAGAAGA AGGGGAGGTT TCAGCTAGCA CAAATGCTCG GAACAAAGCA
ATTACCTCAC TGCTTGGAGG AGGCAGCCCT AAAAATAATA CAGCAGCAGA GACAGAAGAT
GATGAAAGTG ATGGGGAGGA TAGAGGAGGA GGCACTTCAG GGTCATTGAG AAGGTCAAAA
CGAAATTCAG ACTCTACGGA GTTGGCAGCA CAGATGAATG AAAGTGTTGA CGTCATGGAT
GTCATCGCTA TTTGCTGTCC AAAGTACAAA GATCGACCAC AAATTGCAAG AGTAGTGCAG
AAAACCAGCA GTGGCTTCAG TGTTCAGTGG ATGGCAGGCT CCTACAGTGG CTCCTGGACT
GAGGCTAAGC GCCGTGATGG CCGCAAACTG GTGCCTTGGG TAGACACTAT TAAAGAGTCA
GACATTATTT ACAAAAAAAT TGCTCTAACG AGTGCTAATA AGCTGACTAA TAAAGTTGTT
CAGACTTTAC GATCCCTGTA TGCCGCCAAG GATGGGACTT CCAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006714530.1
CDS489..8756
Misc Feature(1)<825..1409
Misc Feature(2)3795..>3968
Misc Feature(3)5868..5993
Misc Feature(4)5919..6005
Misc Feature(5)6030..6110
Misc Feature(6)7314..>7742
Translation

Target ORF information:

RefSeq Version XM_006714467.2
Organism Homo sapiens(Human)
Definition Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006714467.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA
GCATCTATGG TAAAGGACAA AAGGAAAGAG AGAAAATCAT CACCTAGTAA GGAAAATGAG
TCAAGCGACA GTGAAGAAGA AGTTTCCAGG CCTCGGAAGT CACGGAAACG TGTAGATTCA
GATTCAGATT CAGATTCAGA AGACGATATA AATTCAGTGA TGAAATGTTT GCCAGAAAAT
TCAGCTCCTT TAATCGAATT TGCAAATGTG TCCCAGGGTA TTTTATTACT TCTCATGTTA
AAACAACATT TGAAGAATCT TTGTGGATTT TCTGATAGTA AAATTCAGAA GTACTCTCCA
TCTGAATCTG CAAAAGTATA TGATAAAGCG ATAAACCGAA AAACAGGAGT TCATTTTCAT
CCAAAACAAA CACTGGACTT CCTGCGGAGT GACATGGCTA ATTCCAAAAT CACAGAAGAG
GTGAAAAGGA GTATAGTAAA ACAGTATCTA GATTTCAAAC TTCTCATGGA ACATCTGGAC
CCTGATGAAG AAGAAGAAGA AGGGGAGGTT TCAGCTAGCA CAAATGCTCG GAACAAAGCA
ATTACCTCAC TGCTTGGAGG AGGCAGCCCT AAAAATAATA CAGCAGCAGA GACAGAAGAT
GATGAAAGTG ATGGGGAGGA TAGAGGAGGA GGCACTTCAG GGTCATTGAG AAGGTCAAAA
CGAAATTCAG ACTCTACGGA GTTGGCAGCA CAGATGAATG AAAGTGTTGA CGTCATGGAT
GTCATCGCTA TTTGCTGTCC AAAGTACAAA GATCGACCAC AAATTGCAAG AGTAGTGCAG
AAAACCAGCA GTGGCTTCAG TGTTCAGTGG ATGGCAGGCT CCTACAGTGG CTCCTGGACT
GAGGCTAAGC GCCGTGATGG CCGCAAACTG GTGCCTTGGG TAGACACTAT TAAAGAGTCA
GACATTATTT ACAAAAAAAT TGCTCTAACG AGTGCTAATA AGCTGACTAA TAAAGTTGTT
CAGACTTTAC GATCCCTGTA TGCCGCCAAG GATGGGACTT CCAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu39307
Clone ID Related Accession (Same CDS sequence) XM_006714468.2
Accession Version XM_006714468.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8217bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product nipped-B-like protein isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714468.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATTTGCTA GGAAAATGAA GAAAAAAGAA AAACAGAAGA AAAGGAAAGC ATATGAACCA
AAACTAACAC CTGAAGAAAT GATGGACTCT TCAACTTTTA AGAGATTCAC AGCCTCAATA
GAGAATATTT TGGATAATTT GGAAGATATG GATTTTACTG CGTTTGGTGA TGATGATGAA
ATTCCTCAGG AACTGCTCTT AGGAAAACAT CAGCTTAATG AACTTGGCAG TGAATCTGCT
AAAATAAAAG CAATGGGTAT AATGGATAAG CTTTCAACTG ACAAAACTGT GAAAGTCTTA
AATATCTTGG AGAAGAATAT TCAGGATGGG TCAAAGCTTT CCACTTTGTT AAATCATAAT
AACGATACTG AAGAAGAAGA AAGGTTATGG AGAGACCTTA TTATGGAGAG AGTTACAAAA
TCAGCGGATG CTTGTCTTAC AACTATCAAC ATTATGACAT CCCCTAACAT GCCAAAAGCT
GTGTACATTG AGGATGTAAT TGAAAGAGTT ATACAGTACA CTAAATTTCA TTTGCAGAAT
ACACTTTATC CTCAGTATGA TCCTGTTTAC AGATTAGATC CTCATGGAGG AGGCTTATTA
AGTTCAAAAG CAAAACGGGC TAAATGTTCT ACCCATAAGC AGAGAGTAAT AGTAATGCTT
TATAACAAAG TTTGTGACAT TGTTAGCAGC TTATCAGAAT TGCTAGAGAT ACAACTTCTT
ACAGACACAA CAATTCTTCA GGTTTCATCT ATGGGAATAA CACCATTTTT TGTGGAAAAT
GTCAGTGAAC TACAGTTGTG TGCCATTAAG TTAGTCACTG CAGTATTCTC AAGATATGAA
AAACATAGGC AGTTAATTTT GGAAGAAATT TTTACTTCAC TTGCAAGATT ACCAACCAGC
AAGAGGAGTT TAAGGAACTT CAGGTTAAAC AGTAGTGATA TGGATGGAGA ACCTATGTAT
ATTCAGATGG TTACAGCACT GGTTTTACAA CTTATTCAGT GTGTGGTACA CTTACCATCA
TCAGAGAAGG ACTCTAATGC AGAAGAAGAT TCAAATAAAA AAATTGACCA GGATGTTGTC
ATTACTAACT CTTATGAAAC AGCTATGCGA ACAGCCCAAA ACTTCCTCTC CATCTTCCTT
AAAAAATGTG GTAGTAAGCA AGGTGAAGAA GATTACAGAC CACTGTTTGA AAATTTTGTT
CAAGACCTTC TTTCAACAGT CAATAAGCCT GAATGGCCAG CTGCTGAACT ACTCCTTAGT
TTGTTAGGGA GACTGTTGGT TCATCAGTTC AGTAACAAGT CAACAGAGAT GGCTTTAAGA
GTGGCATCTC TTGATTACCT TGGAACTGTT GCTGCACGGC TAAGAAAAGA TGCTGTTACA
AGCAAAATGG ATCAAGGATC TATAGAACGC ATTTTAAAAC AGGTTTCAGG AGGGGAAGAT
GAAATCCAAC AATTACAAAA AGCATTGCTT GATTACTTGG ATGAAAACAC TGAGACTGAT
CCTTCACTAG TGTTTTCTCG TAAATTCTAT ATAGCCCAGT GGTTTCGAGA CACAACTCTG
GAAACAGAAA AAGCAATGAA ATCACAAAAA GATGAAGAAT CATCTGAAGG AACACATCAT
GCAAAGGAAA TTGAGACAAC TGGCCAAATT ATGCATCGAG CTGAAAACCG AAAAAAGTTT
CTTAGAAGCA TTATCAAAAC CACACCTTCT CAGTTTAGCA CATTAAAGAT GAACTCTGAT
ACTGTGGACT ATGATGATGC TTGCTTGATT GTTCGATACT TGGCCTCCAT GAGGCCGTTT
GCCCAGAGCT TTGATATTTA TTTGACACAG ATCCTACGAG TTCTTGGTGA AAATGCAATT
GCTGTTCGAA CAAAAGCCAT GAAGTGTTTG TCTGAGGTTG TTGCTGTAGA CCCCAGTATT
CTAGCAAGGC TTGATATGCA ACGAGGTGTT CATGGACGAT TGATGGATAA TTCGACTAGT
GTCCGAGAAG CAGCAGTAGA ATTACTAGGT CGATTTGTCC TTTGTCGACC TCAGCTTGCT
GAACAGTATT ATGATATGCT GATTGAAAGA ATATTGGATA CTGGTATCAG TGTCAGGAAA
AGAGTAATAA AGATTCTCAG AGACATTTGT ATTGAACAAC CAACATTTCC AAAAATCACA
GAAATGTGTG TAAAAATGAT TCGCAGAGTC AATGATGAAG AGGGCATTAA GAAATTAGTA
AATGAAACAT TCCAGAAACT CTGGTTTACT CCAACTCCAC ACAATGACAA AGAAGCAATG
ACAAGGAAAA TTTTAAACAT TACCGATGTG GTTGCAGCAT GCAGAGATAC TGGATATGAC
TGGTTTGAGC AACTGCTTCA AAACTTGTTG AAGTCCGAAG AGGATTCCTC ATATAAACCT
GTGAAGAAAG CTTGTACTCA ACTTGTTGAT AACCTAGTTG AGCACATTCT TAAATATGAG
GAATCTCTAG CTGACTCTGA CAATAAAGGT GTGAATTCTG GAAGATTGGT AGCTTGCATA
ACCACTTTGT TCTTATTCAG CAAAATAAGA CCCCAGCTCA TGGTTAAACA TGCAATGACT
ATGCAACCAT ACCTTACCAC TAAATGTAGT ACGCAAAATG ATTTCATGGT TATCTGCAAT
GTTGCAAAAA TCCTAGAGCT AGTTGTACCA CTGATGGAGC ATCCAAGTGA AACTTTTCTT
GCCACTATTG AGGAAGATCT AATGAAGCTC ATCATCAAAT ATGGCATGAC TGTAGTGCAA
CATTGTGTGA GCTGTCTTGG AGCTGTTGTA AATAAAGTGA CACAAAATTT TAAATTTGTG
TGGGCTTGTT TCAATAGATA CTATGGTGCC ATTTCAAAAT TAAAAAGTCA ACACCAAGAG
GACCCAAATA ACACTTCACT TCTAACAAAC AAACCAGCAC TTCTTAGATC CCTTTTCACC
GTTGGAGCAC TATGTCGGCA TTTTGATTTT GATCTGGAAG ATTTTAAAGG CAACAGCAAG
GTTAACATAA AAGATAAAGT ACTTGAACTA TTGATGTATT TTACAAAACA CTCAGATGAA
GAAGTACAAA CAAAAGCTAT CATTGGTCTA GGATTTGCCT TTATTCAGCA TCCAAGTCTA
ATGTTCGAGC AAGAAGTGAA GAATCTATAT AATAATATTT TATCTGATAA GAACTCCTCA
GTCAATTTAA AAATACAAGT GTTAAAAAAC CTCCAGACCT ACCTACAAGA AGAAGATACA
CGTATGCAGC AGGCAGATAG AGACTGGAAG AAAGTTGCAA AACAGGAAGA CTTAAAAGAA
ATGGGTGATG TTTCCTCAGG GATGAGTAGT TCCATCATGC AGCTTTATCT CAAACAGGTG
CTTGAGGCAT TTTTTCACAC CCAGTCAAGT GTACGCCACT TTGCCCTAAA TGTCATTGCA
TTGACTCTAA ATCAAGGTCT TATTCATCCA GTTCAGTGTG TGCCATATTT AATTGCTATG
GGCACAGACC CAGAACCTGC TATGCGGAAC AAGGCTGATC AGCAACTTGT GGAAATAGAC
AAAAAATATG CTGGATTCAT TCATATGAAA GCAGTGGCTG GTATGAAGAT GTCTTACCAG
GTACAACAGG CAATCAACAC ATGCCTAAAA GATCCTGTAA GGGGTTTCAG ACAAGACGAG
TCCTCTAGCG CTTTGTGTTC ACACCTTTAC TCCATGATCC GTGGAAACCG CCAACACAGA
CGAGCCTTTC TTATTTCTTT ACTCAACCTC TTTGATGACA CAGCAAAAAC AGACGTGACT
ATGCTCTTGT ATATAGCAGA CAATCTAGCC TGTTTTCCAT ACCAGACACA GGAAGAGCCG
TTGTTTATAA TGCATCATAT AGACATTACA CTCTCAGTTT CTGGTAGTAA CCTACTGCAG
TCATTCAAGG AGTCTATGGT AAAGGACAAA AGGAAAGAGA GAAAATCATC ACCTAGTAAG
GAAAATGAGT CAAGCGACAG TGAAGAAGAA GTTTCCAGGC CTCGGAAGTC ACGGAAACGT
GTAGATTCAG ATTCAGATTC AGATTCAGAA GACGATATAA ATTCAGTGAT GAAATGTTTG
CCAGAAAATT CAGCTCCTTT AATCGAATTT GCAAATGTGT CCCAGGGTAT TTTATTACTT
CTCATGTTAA AACAACATTT GAAGAATCTT TGTGGATTTT CTGATAGTAA AATTCAGAAG
TACTCTCCAT CTGAATCTGC AAAAGTATAT GATAAAGCGA TAAACCGAAA AACAGGAGTT
CATTTTCATC CAAAACAAAC ACTGGACTTC CTGCGGAGTG ACATGGCTAA TTCCAAAATC
ACAGAAGAGG TGAAAAGGAG TATAGTAAAA CAGTATCTAG ATTTCAAACT TCTCATGGAA
CATCTGGACC CTGATGAAGA AGAAGAAGAA GGGGAGGTTT CAGCTAGCAC AAATGCTCGG
AACAAAGCAA TTACCTCACT GCTTGGAGGA GGCAGCCCTA AAAATAATAC AGCAGCAGAG
ACAGAAGATG ATGAAAGTGA TGGGGAGGAT AGAGGAGGAG GCACTTCAGG GTCATTGAGA
AGGTCAAAAC GAAATTCAGA CTCTACGGAG TTGGCAGCAC AGATGAATGA AAGTGTTGAC
GTCATGGATG TCATCGCTAT TTGCTGTCCA AAGTACAAAG ATCGACCACA AATTGCAAGA
GTAGTGCAGA AAACCAGCAG TGGCTTCAGT GTTCAGTGGA TGGCAGGCTC CTACAGTGGC
TCCTGGACTG AGGCTAAGCG CCGTGATGGC CGCAAACTGG TGCCTTGGGT AGACACTATT
AAAGAGTCAG ACATTATTTA CAAAAAAATT GCTCTAACGA GTGCTAATAA GCTGACTAAT
AAAGTTGTTC AGACTTTACG ATCCCTGTAT GCCGCCAAGG ATGGGACTTC CAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006714531.1
CDS489..8705
Misc Feature(1)<825..1409
Misc Feature(2)5670..5795
Misc Feature(3)5721..5807
Misc Feature(4)5832..5912
Misc Feature(5)7116..7652
Translation

Target ORF information:

RefSeq Version XM_006714468.2
Organism Homo sapiens(Human)
Definition Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006714468.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATTTGCTA GGAAAATGAA GAAAAAAGAA AAACAGAAGA AAAGGAAAGC ATATGAACCA
AAACTAACAC CTGAAGAAAT GATGGACTCT TCAACTTTTA AGAGATTCAC AGCCTCAATA
GAGAATATTT TGGATAATTT GGAAGATATG GATTTTACTG CGTTTGGTGA TGATGATGAA
ATTCCTCAGG AACTGCTCTT AGGAAAACAT CAGCTTAATG AACTTGGCAG TGAATCTGCT
AAAATAAAAG CAATGGGTAT AATGGATAAG CTTTCAACTG ACAAAACTGT GAAAGTCTTA
AATATCTTGG AGAAGAATAT TCAGGATGGG TCAAAGCTTT CCACTTTGTT AAATCATAAT
AACGATACTG AAGAAGAAGA AAGGTTATGG AGAGACCTTA TTATGGAGAG AGTTACAAAA
TCAGCGGATG CTTGTCTTAC AACTATCAAC ATTATGACAT CCCCTAACAT GCCAAAAGCT
GTGTACATTG AGGATGTAAT TGAAAGAGTT ATACAGTACA CTAAATTTCA TTTGCAGAAT
ACACTTTATC CTCAGTATGA TCCTGTTTAC AGATTAGATC CTCATGGAGG AGGCTTATTA
AGTTCAAAAG CAAAACGGGC TAAATGTTCT ACCCATAAGC AGAGAGTAAT AGTAATGCTT
TATAACAAAG TTTGTGACAT TGTTAGCAGC TTATCAGAAT TGCTAGAGAT ACAACTTCTT
ACAGACACAA CAATTCTTCA GGTTTCATCT ATGGGAATAA CACCATTTTT TGTGGAAAAT
GTCAGTGAAC TACAGTTGTG TGCCATTAAG TTAGTCACTG CAGTATTCTC AAGATATGAA
AAACATAGGC AGTTAATTTT GGAAGAAATT TTTACTTCAC TTGCAAGATT ACCAACCAGC
AAGAGGAGTT TAAGGAACTT CAGGTTAAAC AGTAGTGATA TGGATGGAGA ACCTATGTAT
ATTCAGATGG TTACAGCACT GGTTTTACAA CTTATTCAGT GTGTGGTACA CTTACCATCA
TCAGAGAAGG ACTCTAATGC AGAAGAAGAT TCAAATAAAA AAATTGACCA GGATGTTGTC
ATTACTAACT CTTATGAAAC AGCTATGCGA ACAGCCCAAA ACTTCCTCTC CATCTTCCTT
AAAAAATGTG GTAGTAAGCA AGGTGAAGAA GATTACAGAC CACTGTTTGA AAATTTTGTT
CAAGACCTTC TTTCAACAGT CAATAAGCCT GAATGGCCAG CTGCTGAACT ACTCCTTAGT
TTGTTAGGGA GACTGTTGGT TCATCAGTTC AGTAACAAGT CAACAGAGAT GGCTTTAAGA
GTGGCATCTC TTGATTACCT TGGAACTGTT GCTGCACGGC TAAGAAAAGA TGCTGTTACA
AGCAAAATGG ATCAAGGATC TATAGAACGC ATTTTAAAAC AGGTTTCAGG AGGGGAAGAT
GAAATCCAAC AATTACAAAA AGCATTGCTT GATTACTTGG ATGAAAACAC TGAGACTGAT
CCTTCACTAG TGTTTTCTCG TAAATTCTAT ATAGCCCAGT GGTTTCGAGA CACAACTCTG
GAAACAGAAA AAGCAATGAA ATCACAAAAA GATGAAGAAT CATCTGAAGG AACACATCAT
GCAAAGGAAA TTGAGACAAC TGGCCAAATT ATGCATCGAG CTGAAAACCG AAAAAAGTTT
CTTAGAAGCA TTATCAAAAC CACACCTTCT CAGTTTAGCA CATTAAAGAT GAACTCTGAT
ACTGTGGACT ATGATGATGC TTGCTTGATT GTTCGATACT TGGCCTCCAT GAGGCCGTTT
GCCCAGAGCT TTGATATTTA TTTGACACAG ATCCTACGAG TTCTTGGTGA AAATGCAATT
GCTGTTCGAA CAAAAGCCAT GAAGTGTTTG TCTGAGGTTG TTGCTGTAGA CCCCAGTATT
CTAGCAAGGC TTGATATGCA ACGAGGTGTT CATGGACGAT TGATGGATAA TTCGACTAGT
GTCCGAGAAG CAGCAGTAGA ATTACTAGGT CGATTTGTCC TTTGTCGACC TCAGCTTGCT
GAACAGTATT ATGATATGCT GATTGAAAGA ATATTGGATA CTGGTATCAG TGTCAGGAAA
AGAGTAATAA AGATTCTCAG AGACATTTGT ATTGAACAAC CAACATTTCC AAAAATCACA
GAAATGTGTG TAAAAATGAT TCGCAGAGTC AATGATGAAG AGGGCATTAA GAAATTAGTA
AATGAAACAT TCCAGAAACT CTGGTTTACT CCAACTCCAC ACAATGACAA AGAAGCAATG
ACAAGGAAAA TTTTAAACAT TACCGATGTG GTTGCAGCAT GCAGAGATAC TGGATATGAC
TGGTTTGAGC AACTGCTTCA AAACTTGTTG AAGTCCGAAG AGGATTCCTC ATATAAACCT
GTGAAGAAAG CTTGTACTCA ACTTGTTGAT AACCTAGTTG AGCACATTCT TAAATATGAG
GAATCTCTAG CTGACTCTGA CAATAAAGGT GTGAATTCTG GAAGATTGGT AGCTTGCATA
ACCACTTTGT TCTTATTCAG CAAAATAAGA CCCCAGCTCA TGGTTAAACA TGCAATGACT
ATGCAACCAT ACCTTACCAC TAAATGTAGT ACGCAAAATG ATTTCATGGT TATCTGCAAT
GTTGCAAAAA TCCTAGAGCT AGTTGTACCA CTGATGGAGC ATCCAAGTGA AACTTTTCTT
GCCACTATTG AGGAAGATCT AATGAAGCTC ATCATCAAAT ATGGCATGAC TGTAGTGCAA
CATTGTGTGA GCTGTCTTGG AGCTGTTGTA AATAAAGTGA CACAAAATTT TAAATTTGTG
TGGGCTTGTT TCAATAGATA CTATGGTGCC ATTTCAAAAT TAAAAAGTCA ACACCAAGAG
GACCCAAATA ACACTTCACT TCTAACAAAC AAACCAGCAC TTCTTAGATC CCTTTTCACC
GTTGGAGCAC TATGTCGGCA TTTTGATTTT GATCTGGAAG ATTTTAAAGG CAACAGCAAG
GTTAACATAA AAGATAAAGT ACTTGAACTA TTGATGTATT TTACAAAACA CTCAGATGAA
GAAGTACAAA CAAAAGCTAT CATTGGTCTA GGATTTGCCT TTATTCAGCA TCCAAGTCTA
ATGTTCGAGC AAGAAGTGAA GAATCTATAT AATAATATTT TATCTGATAA GAACTCCTCA
GTCAATTTAA AAATACAAGT GTTAAAAAAC CTCCAGACCT ACCTACAAGA AGAAGATACA
CGTATGCAGC AGGCAGATAG AGACTGGAAG AAAGTTGCAA AACAGGAAGA CTTAAAAGAA
ATGGGTGATG TTTCCTCAGG GATGAGTAGT TCCATCATGC AGCTTTATCT CAAACAGGTG
CTTGAGGCAT TTTTTCACAC CCAGTCAAGT GTACGCCACT TTGCCCTAAA TGTCATTGCA
TTGACTCTAA ATCAAGGTCT TATTCATCCA GTTCAGTGTG TGCCATATTT AATTGCTATG
GGCACAGACC CAGAACCTGC TATGCGGAAC AAGGCTGATC AGCAACTTGT GGAAATAGAC
AAAAAATATG CTGGATTCAT TCATATGAAA GCAGTGGCTG GTATGAAGAT GTCTTACCAG
GTACAACAGG CAATCAACAC ATGCCTAAAA GATCCTGTAA GGGGTTTCAG ACAAGACGAG
TCCTCTAGCG CTTTGTGTTC ACACCTTTAC TCCATGATCC GTGGAAACCG CCAACACAGA
CGAGCCTTTC TTATTTCTTT ACTCAACCTC TTTGATGACA CAGCAAAAAC AGACGTGACT
ATGCTCTTGT ATATAGCAGA CAATCTAGCC TGTTTTCCAT ACCAGACACA GGAAGAGCCG
TTGTTTATAA TGCATCATAT AGACATTACA CTCTCAGTTT CTGGTAGTAA CCTACTGCAG
TCATTCAAGG AGTCTATGGT AAAGGACAAA AGGAAAGAGA GAAAATCATC ACCTAGTAAG
GAAAATGAGT CAAGCGACAG TGAAGAAGAA GTTTCCAGGC CTCGGAAGTC ACGGAAACGT
GTAGATTCAG ATTCAGATTC AGATTCAGAA GACGATATAA ATTCAGTGAT GAAATGTTTG
CCAGAAAATT CAGCTCCTTT AATCGAATTT GCAAATGTGT CCCAGGGTAT TTTATTACTT
CTCATGTTAA AACAACATTT GAAGAATCTT TGTGGATTTT CTGATAGTAA AATTCAGAAG
TACTCTCCAT CTGAATCTGC AAAAGTATAT GATAAAGCGA TAAACCGAAA AACAGGAGTT
CATTTTCATC CAAAACAAAC ACTGGACTTC CTGCGGAGTG ACATGGCTAA TTCCAAAATC
ACAGAAGAGG TGAAAAGGAG TATAGTAAAA CAGTATCTAG ATTTCAAACT TCTCATGGAA
CATCTGGACC CTGATGAAGA AGAAGAAGAA GGGGAGGTTT CAGCTAGCAC AAATGCTCGG
AACAAAGCAA TTACCTCACT GCTTGGAGGA GGCAGCCCTA AAAATAATAC AGCAGCAGAG
ACAGAAGATG ATGAAAGTGA TGGGGAGGAT AGAGGAGGAG GCACTTCAGG GTCATTGAGA
AGGTCAAAAC GAAATTCAGA CTCTACGGAG TTGGCAGCAC AGATGAATGA AAGTGTTGAC
GTCATGGATG TCATCGCTAT TTGCTGTCCA AAGTACAAAG ATCGACCACA AATTGCAAGA
GTAGTGCAGA AAACCAGCAG TGGCTTCAGT GTTCAGTGGA TGGCAGGCTC CTACAGTGGC
TCCTGGACTG AGGCTAAGCG CCGTGATGGC CGCAAACTGG TGCCTTGGGT AGACACTATT
AAAGAGTCAG ACATTATTTA CAAAAAAATT GCTCTAACGA GTGCTAATAA GCTGACTAAT
AAAGTTGTTC AGACTTTACG ATCCCTGTAT GCCGCCAAGG ATGGGACTTC CAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu39308
Clone ID Related Accession (Same CDS sequence) XM_005248280.3
Accession Version XM_005248280.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8097bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product nipped-B-like protein isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005248280.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC TCATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005248337.1
CDS489..8585
Misc Feature(1)<825..1409
Misc Feature(2)3795..>3968
Misc Feature(3)5868..5993
Misc Feature(4)5919..6005
Misc Feature(5)6030..6110
Misc Feature(6)7314..7850
Translation

Target ORF information:

RefSeq Version XM_005248280.3
Organism Homo sapiens(Human)
Definition Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005248280.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC TCATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu66530
Clone ID Related Accession (Same CDS sequence) XM_011514015.1
Accession Version XM_011514015.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 7266bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product nipped-B-like protein isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA
GCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011512317.1
CDS489..7754
Misc Feature(1)<825..1409
Misc Feature(2)3795..>3968
Misc Feature(3)5868..5993
Misc Feature(4)5919..6005
Misc Feature(5)6030..6110
Misc Feature(6)7314..>7742
Translation

Target ORF information:

RefSeq Version XM_011514015.1
Organism Homo sapiens(Human)
Definition Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011514015.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA
GCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

mRNA Quantification of NIPBL Isoforms A and B in Adult and Fetal Human Tissues, and a Potentially Pathological Variant Affecting Only Isoform A in Two Patients with Cornelia de Lange Syndrome.
International journal of molecular sciences18(3)(2017 Feb)
Puisac B,Teresa-Rodrigo ME,Hernández-Marcos M,Baquero-Montoya C,Gil-Rodríguez MC,Visnes T,Bot C,Gómez-Puertas P,Kaiser FJ,Ramos FJ,Ström L,Pié J


book

A series of 38 novel germline and somatic mutations of NIPBL in Cornelia de Lange syndrome.
Clinical genetics89(5)584-9(2016 May)
Nizon M,Henry M,Michot C,Baumann C,Bazin A,Bessières B,Blesson S,Cordier-Alex MP,David A,Delahaye-Duriez A,Delezoïde AL,Dieux-Coeslier A,Doco-Fenzy M,Faivre L,Goldenberg A,Layet V,Loget P,Marlin S,Martinovic J,Odent S,Pasquier L,Plessis G,Prieur F,Putoux A,Rio M,Testard H,Bonnefont JP,Cormier-Daire V


book

NIPBL Controls RNA Biogenesis to Prevent Activation of the Stress Kinase PKR.
Cell reports14(1)93-102(2016 Jan)
Yuen KC,Xu B,Krantz ID,Gerton JL


book

Identification and Functional Characterization of Two Intronic NIPBL Mutations in Two Patients with Cornelia de Lange Syndrome.
BioMed research international20168742939(2016)
Teresa-Rodrigo ME,Eckhold J,Puisac B,Pozojevic J,Parenti I,Baquero-Montoya C,Gil-Rodríguez MC,Braunholz D,Dalski A,Hernández-Marcos M,Ayerza A,Bernal ML,Ramos FJ,Wieczorek D,Gillessen-Kaesbach G,Pié J,Kaiser FJ


 
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