EEF1AKMT3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Gene Symbol	EEF1AKMT3
Entrez Gene ID	25895
Full Name	EEF1A lysine methyltransferase 3
Synonyms	FAM119B,METTL21B
General protein information	Preferred Names EEF1A lysine methyltransferase 3 Names EEF1A lysine methyltransferase 3 family with sequence similarity 119, member B hepatocellular carcinoma-associated antigen 557a hepatocellularcarcinoma-associated antigen HCA557a methyltransferase like 21B methyltransferase-like protein 21B protein-lysine methyltransferase METTL21B
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q14.1
Disorder MIM:	615258

mRNA and Protein(s)

mRNA	Protein	Name
NM_015433.2	NP_056248.2	EEF1A lysine methyltransferase 3 isoform a
NM_206914.1	NP_996797.1	EEF1A lysine methyltransferase 3 isoform b
NM_206914.2	NP_996797.1	EEF1A lysine methyltransferase 3 isoform b
NM_015433.3	NP_056248.2	EEF1A lysine methyltransferase 3 isoform a

Pathways from BioSystems

Homologies

Homo sapiens (human)	METTL21B	NP_056248.2
	METTL21B	XP_002798696.1
Mus musculus (house mouse)	LOC100504608	NP_001191965.1
Pan troglodytes (chimpanzee)	METTL21B	XP_003313593.1
Canis lupus familiaris (dog)	METTL21B	XP_003431507.1
Rattus norvegicus (Norway rat)	LOC679247	XP_003750376.2
Bos taurus (cattle)	METTL21B	NP_001096810.1
Xenopus tropicalis (tropical clawed frog)	mettl21b	NP_001016660.1

Gene Ontology
Bibliography

Related articles in PubMed

METTL21B Is a Novel Human Lysine Methyltransferase of Translation Elongation Factor 1A: Discovery by CRISPR/Cas9 Knockout.
Hamey JJ, Wienert B, Quinlan KGR, Wilkins MR
Molecular & cellular proteomics : MCP16(12)2229-2242(2017 Dec)

The novel lysine specific methyltransferase METTL21B affects mRNA translation through inducible and dynamic methylation of Lys-165 in human eukaryotic elongation factor 1 alpha (eEF1A).
Malecki J, Aileni VK, Ho AYY, Schwarz J, Moen A, S?rensen V, Nilges BS, Jakobsson ME, Leidel SA, Falnes P?
Nucleic acids research45(8)4370-4389(2017 May)

A newly uncovered group of distantly related lysine methyltransferases preferentially interact with molecular chaperones to regulate their activity.
Cloutier P, Lavall?e-Adam M, Faubert D, Blanchette M, Coulombe B
PLoS genetics9(1)e1003210(2013)

Lysine methylation of VCP by a member of a novel human protein methyltransferase family.
Kernstock S, Davydova E, Jakobsson M, Moen A, Pettersen S, M?landsmo GM, Egge-Jacobsen W, Falnes P?
Nature communications31038(2012)

The multiple sclerosis whole blood mRNA transcriptome and genetic associations indicate dysregulation of specific T cell pathways in pathogenesis.
Gandhi KS, McKay FC, Cox M, Riveros C, Armstrong N, Heard RN, Vucic S, Williams DW, Stankovich J, Brown M, Danoy P, Stewart GJ, Broadley S, Moscato P, Lechner-Scott J, Scott RJ, Booth DR,
Human molecular genetics19(11)2134-43(2010 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The present study identifies METTL21B as the enzyme responsible for methylation of eEF1A on Lys-165 and shows that this modification is dynamic, inducible and likely of regulatory importance.
Title: The novel lysine specific methyltransferase METTL21B affects mRNA translation through inducible and dynamic methylation of Lys-165 in human eukaryotic elongation factor 1 alpha (eEF1A).

The METTL21B protein is here suggested to be a protein methyltransferase, as it is shown to belong to a family of 10 human methyltransferases, some of which are shown to have lysine specific protein methyltransferase activity.
Title: Lysine methylation of VCP by a member of a novel human protein methyltransferase family.

Observational study and genome-wide association study of gene-disease association. (HuGE Navigator)
Title: The multiple sclerosis whole blood mRNA transcriptome and genetic associations indicate dysregulation of specific T cell pathways in pathogenesis.

The following EEF1AKMT3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EEF1AKMT3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17106	NM_015433.3 Latest version!	Homo sapiens EEF1A lysine methyltransferase 3 (EEF1AKMT3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu17069	NM_206914.2 Latest version!	Homo sapiens EEF1A lysine methyltransferase 3 (EEF1AKMT3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu17069	NM_206914.1	Homo sapiens EEF1A lysine methyltransferase 3 (EEF1AKMT3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu17106	NM_015433.2	Homo sapiens EEF1A lysine methyltransferase 3 (EEF1AKMT3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EEF1AKMT3 ( NM_015433.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_056248.2 Homo sapiens EEF1A lysine methyltransferase 3 (EEF1AKMT3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17106
Clone ID Related Accession (Same CDS sequence)	NM_015433.2 , NM_015433.3
Accession Version	NM_015433.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-17
Organism	Homo sapiens(human)
Product	EEF1A lysine methyltransferase 3 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC016395.1 and AL050100.1. On Mar 30, 2004 this sequence version replaced NM_015433.1. Transcript Variant: This variant (1) encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: BC016395.1, SRR1803611.110587.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACC CCGGCCCAGA TCCCGAATCT GAGTCGGAAT CGGTGTTCCC GCGGGAGGTC 
GGGCTCTTTG CAGACTCTTA CTCGGAGAAG AGCCAGTTCT GTTTCTGTGG GCATGTGCTG 
ACCATCACGC AGAACTTTGG GTCCCGCCTC GGGGTGGCGG CGCGCGTGTG GGACGCGGCC 
CTGAGCCTGT GCAATTATTT CGAGAGTCAA AATGTGGATT TCCGAGGCAA GAAGGTGATC 
GAACTGGGTG CGGGGACAGG CATCGTGGGG ATCTTGGCAG CGCTGCAGGG GGGGGATGTT 
ACCATCACTG ACCTGCCCCT GGCCCTAGAA CAGATCCAGG GCAACGTCCA GGCCAATGTG 
CCAGCTGGAG GCCAGGCCCA GGTGCGTGCC TTGTCCTGGG GGATTGACCA TCATGTCTTC 
CCTGCAAACT ATGACCTGGT GCTGGGGGCT GATATCGTGT ACCTGGAACC CACCTTCCCT 
CTGCTGCTGG GGACCCTCCA ACACCTGTGC AGGCCCCATG GCACCATCTA TCTGGCCTCC 
AAGATGAGAA AGGAGCATGG GACAGAGAGC TTCTTTCAGC ACCTCCTGCC CCAGCATTTC 
CAACTGGAGC TGGCTCAGCG GGATGAGGAT GAAAATGTCA ACATCTATAG GGCCAGGCAC 
AGGGAACCAA GACCTGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056248.2
CDS	126..806
Translation	MADPGPDPESESESVFPREVGLFADSYSEKSQFCFCGHVLTITQNFGSRLGVAARVWDAALSLCNYFESQNVDFRGKKVIELGAGTGIVGILAALQGGDVTITDLPLALEQIQGNVQANVPAGGQAQVRALSWGIDHHVFPANYDLVLGADIVYLEPTFPLLLGTLQHLCRPHGTIYLASKMRKEHGTESFFQHLLPQHFQLELAQRDEDENVNIYRARHREPRPA

Target ORF information:

RefSeq Version	NM_015433.2
Organism	Homo sapiens(human)
Definition	Homo sapiens EEF1A lysine methyltransferase 3 (EEF1AKMT3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015433.2

ORF Insert Sequence:

ATGGCGGACC CCGGCCCAGA TCCCGAATCT GAGTCGGAAT CGGTGTTCCC GCGGGAGGTC 
GGGCTCTTTG CAGACTCTTA CTCGGAGAAG AGCCAGTTCT GTTTCTGTGG GCATGTGCTG 
ACCATCACGC AGAACTTTGG GTCCCGCCTC GGGGTGGCGG CGCGCGTGTG GGACGCGGCC 
CTGAGCCTGT GCAATTATTT CGAGAGTCAA AATGTGGATT TCCGAGGCAA GAAGGTGATC 
GAACTGGGTG CGGGGACAGG CATCGTGGGG ATCTTGGCAG CGCTGCAGGG GGGGGATGTT 
ACCATCACTG ACCTGCCCCT GGCCCTAGAA CAGATCCAGG GCAACGTCCA GGCCAATGTG 
CCAGCTGGAG GCCAGGCCCA GGTGCGTGCC TTGTCCTGGG GGATTGACCA TCATGTCTTC 
CCTGCAAACT ATGACCTGGT GCTGGGGGCT GATATCGTGT ACCTGGAACC CACCTTCCCT 
CTGCTGCTGG GGACCCTCCA ACACCTGTGC AGGCCCCATG GCACCATCTA TCTGGCCTCC 
AAGATGAGAA AGGAGCATGG GACAGAGAGC TTCTTTCAGC ACCTCCTGCC CCAGCATTTC 
CAACTGGAGC TGGCTCAGCG GGATGAGGAT GAAAATGTCA ACATCTATAG GGCCAGGCAC 
AGGGAACCAA GACCTGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEF1AKMT3 ( NM_015433.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_056248.2 Homo sapiens EEF1A lysine methyltransferase 3 (EEF1AKMT3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17106
Clone ID Related Accession (Same CDS sequence)	NM_015433.2 , NM_015433.3
Accession Version	NM_015433.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	EEF1A lysine methyltransferase 3 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC016395.1 and AL050100.1. On Nov 23, 2018 this sequence version replaced NM_015433.2. Transcript Variant: This variant (1) encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: DA938041.1, SRR1803611.110587.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000300209.13/ ENSP00000300209.8 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGACC CCGGCCCAGA TCCCGAATCT GAGTCGGAAT CGGTGTTCCC GCGGGAGGTC 
GGGCTCTTTG CAGACTCTTA CTCGGAGAAG AGCCAGTTCT GTTTCTGTGG GCATGTGCTG 
ACCATCACGC AGAACTTTGG GTCCCGCCTC GGGGTGGCGG CGCGCGTGTG GGACGCGGCC 
CTGAGCCTGT GCAATTATTT CGAGAGTCAA AATGTGGATT TCCGAGGCAA GAAGGTGATC 
GAACTGGGTG CGGGGACAGG CATCGTGGGG ATCTTGGCAG CGCTGCAGGG GGGGGATGTT 
ACCATCACTG ACCTGCCCCT GGCCCTAGAA CAGATCCAGG GCAACGTCCA GGCCAATGTG 
CCAGCTGGAG GCCAGGCCCA GGTGCGTGCC TTGTCCTGGG GGATTGACCA TCATGTCTTC 
CCTGCAAACT ATGACCTGGT GCTGGGGGCT GATATCGTGT ACCTGGAACC CACCTTCCCT 
CTGCTGCTGG GGACCCTCCA ACACCTGTGC AGGCCCCATG GCACCATCTA TCTGGCCTCC 
AAGATGAGAA AGGAGCATGG GACAGAGAGC TTCTTTCAGC ACCTCCTGCC CCAGCATTTC 
CAACTGGAGC TGGCTCAGCG GGATGAGGAT GAAAATGTCA ACATCTATAG GGCCAGGCAC 
AGGGAACCAA GACCTGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056248.2
CDS	112..792
Translation	MADPGPDPESESESVFPREVGLFADSYSEKSQFCFCGHVLTITQNFGSRLGVAARVWDAALSLCNYFESQNVDFRGKKVIELGAGTGIVGILAALQGGDVTITDLPLALEQIQGNVQANVPAGGQAQVRALSWGIDHHVFPANYDLVLGADIVYLEPTFPLLLGTLQHLCRPHGTIYLASKMRKEHGTESFFQHLLPQHFQLELAQRDEDENVNIYRARHREPRPA

Target ORF information:

RefSeq Version	NM_015433.3
Organism	Homo sapiens(human)
Definition	Homo sapiens EEF1A lysine methyltransferase 3 (EEF1AKMT3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015433.3

ORF Insert Sequence:

ATGGCGGACC CCGGCCCAGA TCCCGAATCT GAGTCGGAAT CGGTGTTCCC GCGGGAGGTC 
GGGCTCTTTG CAGACTCTTA CTCGGAGAAG AGCCAGTTCT GTTTCTGTGG GCATGTGCTG 
ACCATCACGC AGAACTTTGG GTCCCGCCTC GGGGTGGCGG CGCGCGTGTG GGACGCGGCC 
CTGAGCCTGT GCAATTATTT CGAGAGTCAA AATGTGGATT TCCGAGGCAA GAAGGTGATC 
GAACTGGGTG CGGGGACAGG CATCGTGGGG ATCTTGGCAG CGCTGCAGGG GGGGGATGTT 
ACCATCACTG ACCTGCCCCT GGCCCTAGAA CAGATCCAGG GCAACGTCCA GGCCAATGTG 
CCAGCTGGAG GCCAGGCCCA GGTGCGTGCC TTGTCCTGGG GGATTGACCA TCATGTCTTC 
CCTGCAAACT ATGACCTGGT GCTGGGGGCT GATATCGTGT ACCTGGAACC CACCTTCCCT 
CTGCTGCTGG GGACCCTCCA ACACCTGTGC AGGCCCCATG GCACCATCTA TCTGGCCTCC 
AAGATGAGAA AGGAGCATGG GACAGAGAGC TTCTTTCAGC ACCTCCTGCC CCAGCATTTC 
CAACTGGAGC TGGCTCAGCG GGATGAGGAT GAAAATGTCA ACATCTATAG GGCCAGGCAC 
AGGGAACCAA GACCTGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEF1AKMT3 ( NM_206914.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_996797.1 Homo sapiens EEF1A lysine methyltransferase 3 (EEF1AKMT3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu17069
Clone ID Related Accession (Same CDS sequence)	NM_206914.1 , NM_206914.2
Accession Version	NM_206914.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Homo sapiens(human)
Product	EEF1A lysine methyltransferase 3 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC016395.1 and AL050100.1. Transcript Variant: This variant (2) contains an alternate coding exon compared to variant 1, that causes a frameshift. The resulting isoform (b) is shorter and has a distinct C-terminus compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: AL050100.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACC CCGGCCCAGA TCCCGAATCT GAGTCGGAAT CGGTGTTCCC GCGGGAGGTC 
GGGCTCTTTG CAGACTCTTA CTCGGAGAAG AGCCAGTTCT GTTTCTGTGG GCATGTGCTG 
ACCATCACGC AGAACTTTGG GTCCCGCCTC GGGGTGGCGG CGCGCGTGTG GGACGCGGCC 
CTGAGCCTGT GCAATTATTT CGAGAGTCAA AATGTGGATT TCCGAGGCAA GAAGGTGATC 
GAACTGGGTG CGGGGACAGG CATCGTGGGG ATCTTGGCAG CGCTGCAGGG AGCTTATGGT 
CTAGTGAGGG AGACAGAGGA CGATGTCATA GAACAAGAAC TCTGGCGAGG TATGCGTGGA 
GCCTGTGGAC ATGCTCTATC CATGTCTACC ATGACTCCCT GGGAATCCAT CAAGGGAAGC 
TCTGTCAGGG GGGGATGTTA CCATCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996797.1
CDS	126..575
Translation	MADPGPDPESESESVFPREVGLFADSYSEKSQFCFCGHVLTITQNFGSRLGVAARVWDAALSLCNYFESQNVDFRGKKVIELGAGTGIVGILAALQGAYGLVRETEDDVIEQELWRGMRGACGHALSMSTMTPWESIKGSSVRGGCYHH

Target ORF information:

RefSeq Version	NM_206914.1
Organism	Homo sapiens(human)
Definition	Homo sapiens EEF1A lysine methyltransferase 3 (EEF1AKMT3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206914.1

ORF Insert Sequence:

ATGGCGGACC CCGGCCCAGA TCCCGAATCT GAGTCGGAAT CGGTGTTCCC GCGGGAGGTC 
GGGCTCTTTG CAGACTCTTA CTCGGAGAAG AGCCAGTTCT GTTTCTGTGG GCATGTGCTG 
ACCATCACGC AGAACTTTGG GTCCCGCCTC GGGGTGGCGG CGCGCGTGTG GGACGCGGCC 
CTGAGCCTGT GCAATTATTT CGAGAGTCAA AATGTGGATT TCCGAGGCAA GAAGGTGATC 
GAACTGGGTG CGGGGACAGG CATCGTGGGG ATCTTGGCAG CGCTGCAGGG AGCTTATGGT 
CTAGTGAGGG AGACAGAGGA CGATGTCATA GAACAAGAAC TCTGGCGAGG TATGCGTGGA 
GCCTGTGGAC ATGCTCTATC CATGTCTACC ATGACTCCCT GGGAATCCAT CAAGGGAAGC 
TCTGTCAGGG GGGGATGTTA CCATCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEF1AKMT3 ( NM_206914.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_996797.1 Homo sapiens EEF1A lysine methyltransferase 3 (EEF1AKMT3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu17069
Clone ID Related Accession (Same CDS sequence)	NM_206914.1 , NM_206914.2
Accession Version	NM_206914.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	EEF1A lysine methyltransferase 3 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC016395.1 and AL050100.1. On May 31, 2019 this sequence version replaced NM_206914.1. Transcript Variant: This variant (2) contains an alternate coding exon compared to variant 1, that causes a frameshift. The resulting isoform (b) is shorter and has a distinct C-terminus compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: AL050100.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACC CCGGCCCAGA TCCCGAATCT GAGTCGGAAT CGGTGTTCCC GCGGGAGGTC 
GGGCTCTTTG CAGACTCTTA CTCGGAGAAG AGCCAGTTCT GTTTCTGTGG GCATGTGCTG 
ACCATCACGC AGAACTTTGG GTCCCGCCTC GGGGTGGCGG CGCGCGTGTG GGACGCGGCC 
CTGAGCCTGT GCAATTATTT CGAGAGTCAA AATGTGGATT TCCGAGGCAA GAAGGTGATC 
GAACTGGGTG CGGGGACAGG CATCGTGGGG ATCTTGGCAG CGCTGCAGGG AGCTTATGGT 
CTAGTGAGGG AGACAGAGGA CGATGTCATA GAACAAGAAC TCTGGCGAGG TATGCGTGGA 
GCCTGTGGAC ATGCTCTATC CATGTCTACC ATGACTCCCT GGGAATCCAT CAAGGGAAGC 
TCTGTCAGGG GGGGATGTTA CCATCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996797.1
CDS	112..561
Translation	MADPGPDPESESESVFPREVGLFADSYSEKSQFCFCGHVLTITQNFGSRLGVAARVWDAALSLCNYFESQNVDFRGKKVIELGAGTGIVGILAALQGAYGLVRETEDDVIEQELWRGMRGACGHALSMSTMTPWESIKGSSVRGGCYHH

Target ORF information:

RefSeq Version	NM_206914.2
Organism	Homo sapiens(human)
Definition	Homo sapiens EEF1A lysine methyltransferase 3 (EEF1AKMT3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206914.2

ORF Insert Sequence:

ATGGCGGACC CCGGCCCAGA TCCCGAATCT GAGTCGGAAT CGGTGTTCCC GCGGGAGGTC 
GGGCTCTTTG CAGACTCTTA CTCGGAGAAG AGCCAGTTCT GTTTCTGTGG GCATGTGCTG 
ACCATCACGC AGAACTTTGG GTCCCGCCTC GGGGTGGCGG CGCGCGTGTG GGACGCGGCC 
CTGAGCCTGT GCAATTATTT CGAGAGTCAA AATGTGGATT TCCGAGGCAA GAAGGTGATC 
GAACTGGGTG CGGGGACAGG CATCGTGGGG ATCTTGGCAG CGCTGCAGGG AGCTTATGGT 
CTAGTGAGGG AGACAGAGGA CGATGTCATA GAACAAGAAC TCTGGCGAGG TATGCGTGGA 
GCCTGTGGAC ATGCTCTATC CATGTCTACC ATGACTCCCT GGGAATCCAT CAAGGGAAGC 
TCTGTCAGGG GGGGATGTTA CCATCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

METTL21B Is a Novel Human Lysine Methyltransferase of Translation Elongation Factor 1A: Discovery by CRISPR/Cas9 Knockout.
Molecular & cellular proteomics : MCP16(12)2229-2242(2017 Dec)
Hamey JJ,Wienert B,Quinlan KGR,Wilkins MR

The novel lysine specific methyltransferase METTL21B affects mRNA translation through inducible and dynamic methylation of Lys-165 in human eukaryotic elongation factor 1 alpha (eEF1A).
Nucleic acids research45(8)4370-4389(2017 May)
Malecki J,Aileni VK,Ho AYY,Schwarz J,Moen A,S?rensen V,Nilges BS,Jakobsson ME,Leidel SA,Falnes P?

A newly uncovered group of distantly related lysine methyltransferases preferentially interact with molecular chaperones to regulate their activity.
PLoS genetics9(1)e1003210(2013)
Cloutier P,Lavall?e-Adam M,Faubert D,Blanchette M,Coulombe B

Lysine methylation of VCP by a member of a novel human protein methyltransferase family.
Nature communications31038(2012)
Kernstock S,Davydova E,Jakobsson M,Moen A,Pettersen S,M?landsmo GM,Egge-Jacobsen W,Falnes P?

The multiple sclerosis whole blood mRNA transcriptome and genetic associations indicate dysregulation of specific T cell pathways in pathogenesis.
Human molecular genetics19(11)2134-43(2010 Jun)
Gandhi KS,McKay FC,Cox M,Riveros C,Armstrong N,Heard RN,Vucic S,Williams DW,Stankovich J,Brown M,Danoy P,Stewart GJ,Broadley S,Moscato P,Lechner-Scott J,Scott RJ,Booth DR,

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EEF1AKMT3 cDNA ORF clone, Homo sapiens(human)

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

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