EEF1AKMT3 cDNA ORF clone, Homo sapiens(human)
| Gene Symbol | EEF1AKMT3 |
| Entrez Gene ID | 25895 |
| Full Name | EEF1A lysine methyltransferase 3 |
| Synonyms | FAM119B,METTL21B |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Homo sapiens(human) |
| Genome | |
| Disorder MIM: |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_206914.1 | NP_996797.1 | EEF1A lysine methyltransferase 3 isoform b |
| NM_015433.2 | NP_056248.2 | EEF1A lysine methyltransferase 3 isoform a |
| Homo sapiens (human) | METTL21B | NP_056248.2 |
| METTL21B | XP_002798696.1 | |
| Mus musculus (house mouse) | LOC100504608 | NP_001191965.1 |
| Pan troglodytes (chimpanzee) | METTL21B | XP_003313593.1 |
| Canis lupus familiaris (dog) | METTL21B | XP_003431507.1 |
| Rattus norvegicus (Norway rat) | LOC679247 | XP_003750376.2 |
| Bos taurus (cattle) | METTL21B | NP_001096810.1 |
| Xenopus tropicalis (tropical clawed frog) | mettl21b | NP_001016660.1 |
Related articles in PubMed
The novel lysine specific methyltransferase METTL21B affects mRNA translation through inducible and dynamic methylation of Lys-165 in human eukaryotic elongation factor 1 alpha (eEF1A).
Malecki J, Aileni VK, Ho AYY, Schwarz J, Moen A, S?rensen V, Nilges BS, Jakobsson ME, Leidel SA, Falnes P?
Nucleic acids research45(8)4370-4389(2017 May)
A newly uncovered group of distantly related lysine methyltransferases preferentially interact with molecular chaperones to regulate their activity.
Cloutier P, Lavall?e-Adam M, Faubert D, Blanchette M, Coulombe B
PLoS genetics9(1)e1003210(2013)
Lysine methylation of VCP by a member of a novel human protein methyltransferase family.
Kernstock S, Davydova E, Jakobsson M, Moen A, Pettersen S, M?landsmo GM, Egge-Jacobsen W, Falnes P?
Nature communications31038(2012)
The multiple sclerosis whole blood mRNA transcriptome and genetic associations indicate dysregulation of specific T cell pathways in pathogenesis.
Gandhi KS, McKay FC, Cox M, Riveros C, Armstrong N, Heard RN, Vucic S, Williams DW, Stankovich J, Brown M, Danoy P, Stewart GJ, Broadley S, Moscato P, Lechner-Scott J, Scott RJ, Booth DR,
Human molecular genetics19(11)2134-43(2010 Jun)
Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing.
Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S
EMBO reports1(3)287-92(2000 Sep)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
The present study identifies METTL21B as the enzyme responsible for methylation of eEF1A on Lys-165 and shows that this modification is dynamic, inducible and likely of regulatory importance.
Title: The novel lysine specific methyltransferase METTL21B affects mRNA translation through inducible and dynamic methylation of Lys-165 in human eukaryotic elongation factor 1 alpha (eEF1A).
The METTL21B protein is here suggested to be a protein methyltransferase, as it is shown to belong to a family of 10 human methyltransferases, some of which are shown to have lysine specific protein methyltransferase activity.
Title: Lysine methylation of VCP by a member of a novel human protein methyltransferase family.
Observational study and genome-wide association study of gene-disease association. (HuGE Navigator)
Title: The multiple sclerosis whole blood mRNA transcriptome and genetic associations indicate dysregulation of specific T cell pathways in pathogenesis.
The following EEF1AKMT3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EEF1AKMT3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OHu17106 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_015433.2 | |
| Accession Version | NM_015433.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | COA |
| ORF Insert Method | CloneEZ® Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-09-18 | |
| Organism | Homo sapiens(human) | |
| Product | EEF1A lysine methyltransferase 3 isoform a | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC016395.1 and AL050100.1. On Mar 30, 2004 this sequence version replaced NM_015433.1. Transcript Variant: This variant (1) encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: BC016395.1, SRR1803611.110587.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGCGGACC CCGGCCCAGA TCCCGAATCT GAGTCGGAAT CGGTGTTCCC GCGGGAGGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_056248.2 |
| CDS | 126..806 |
| Misc Feature(1) | 234..710 |
| Misc Feature(2) | 372..380 |
| Exon (1) | 1..302 |
| Exon (2) | 303..414 |
| Exon (3) | 415..2701 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_015433.2 |
ORF Insert Sequence:
1 | ATGGCGGACC CCGGCCCAGA TCCCGAATCT GAGTCGGAAT CGGTGTTCCC GCGGGAGGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu17069 | |
| Clone ID Related Accession (Same CDS sequence) | NM_206914.1 | |
| Accession Version | NM_206914.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 450bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ® Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-09-18 | |
| Organism | Homo sapiens(human) | |
| Product | EEF1A lysine methyltransferase 3 isoform b | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC016395.1 and AL050100.1. Transcript Variant: This variant (2) contains an alternate coding exon compared to variant 1, that causes a frameshift. The resulting isoform (b) is shorter and has a distinct C-terminus compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: AL050100.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGCGGACC CCGGCCCAGA TCCCGAATCT GAGTCGGAAT CGGTGTTCCC GCGGGAGGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_996797.1 |
| CDS | 126..575 |
| Misc Feature(1) | 234..>458 |
| Misc Feature(2) | 372..380 |
| Exon (1) | 1..302 |
| Exon (2) | 303..414 |
| Exon (3) | 415..553 |
| Exon (4) | 554..2840 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_206914.1 |
ORF Insert Sequence:
1 | ATGGCGGACC CCGGCCCAGA TCCCGAATCT GAGTCGGAAT CGGTGTTCCC GCGGGAGGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
The novel lysine specific methyltransferase METTL21B affects mRNA translation through inducible and dynamic methylation of Lys-165 in human eukaryotic elongation factor 1 alpha (eEF1A). |
|
A newly uncovered group of distantly related lysine methyltransferases preferentially interact with molecular chaperones to regulate their activity. |
|
Lysine methylation of VCP by a member of a novel human protein methyltransferase family. |
|
The multiple sclerosis whole blood mRNA transcriptome and genetic associations indicate dysregulation of specific T cell pathways in pathogenesis. |
|
Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing. |